Optimization of 13C direct detection NMR methods.
(13)C-detected experiments are still limited by their inherently lower sensitivity, as compared to the equivalent (1)H-detected experiments. Improving the sensitivity of (13)C detection methods remains a significant area of NMR research that may provide better means for studying large macromolecular systems by NMR. In this communication, we show that (13)C-detected experiments are less sensitive to the salt concentration of the sample solution than (1)H-detected experiments. In addition, acquisition can be started with anti-phase coherence, resulting in higher sensitivity due to the elimination of the final INEPT transfer step.
Shimba
Nobuhisa
N
University of California San Francisco, San Francisco, California 94143, USA.
Kovacs
Helena
H
Stern
Alan S
AS
Nomura
Anson M
AM
Shimada
Ichio
I
Hoch
Jeffrey C
JC
Craik
Charles S
CS
Dötsch
Volker
V
D002247:Carbon Isotopes; D007930:Leucine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000494:pharmacology; D012996:Solutions / Q000737:chemistry
D002247:Carbon Isotopes; D012996:Solutions; D012965:Sodium Chloride; D007930:Leucine