title: Effects of multiple dentinal lesions on the rat pulp.
abstract: The purpose of this study was to compare the effects of single and multiple dentinal lesions on the dental pulps of rats. 3H-proline was used to measure pulpal reactionary dentin deposition, and 3H-thymidine was used to measure pulpal cell proliferation by radioautography. Data were compared by factorial analysis of variance and a post-hoc Tukey test. A significant increase in radiolabeled collagenous proteins within reactionary dentin and pulpal cell labeling indices were evident coincident to single dentinal injuries, compared with controls (p < 0.001). After double injuries, the radiolabeled collagenous proteins within reactionary dentin and the pulpal labeling indices remained significantly greater than in controls (p < 0.01); however, each was significantly less than after single injuries (p < 0.05). These data suggest that pulpal cell proliferation and collagenous protein deposition into reactionary dentin are decreased subsequent to a previous dentinal injury. Thus, the healing potential of the dental pulp could be decreased by recent tooth cavity preparation.
authors.affiliation: Department of Periodontics, University of Mississippi Medical Center, School of Dentistry, Jackson, Mississippi 39216-4505, USA. rjohnson@sod.umsmed.edu
authors.forename: R B
authors.initials: RB
authors.lastname: Johnson
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D003737:Dental Cavity Preparation / Q000009:adverse effects*; D003782:Dental Pulp / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D003804:Dentin / Q000293:injuries*; D003809:Dentin, Secondary / Q000378:metabolism*; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric
chemical_list: D003094:Collagen