title: [A study on expression of hsp70 mRNA in the early stage of diffuse brain injury in rats].
abstract: OBJECTIVE
  To evaluate the changes of hsp70 mRNA in neuron and glia of rat suffering early diffuse brain injury.
  
  
  METHODS
  SD rats were used in the replication of the animal pattern of diffuse brain injury. In situ hybridization technique and image analysis technique were applied in detecting the hsp70 mRNA in the rat's cerebral cortex, thalamus and brain stem.
  
  
  RESULTS
  The signal intensity of hsp70 mRNA could be detected in 5 minutes after brain injury; subsequently, it attained to the summit at 6 hours and declined at 12 hours after brain injury.
  
  
  CONCLUSIONS
  The above data suggest that hsp70 mRNA is useful for the diagnosis of early diffuse brain injury and for distinguishing the postmortem injury from antemortem injury.
authors.affiliation: Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong-fu
authors.initials: HF
authors.lastname: Pan
authors.forename: Kui-ming
authors.initials: KM
authors.lastname: Zhang
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Chen
authors.forename: Qi-yi
authors.initials: QY
authors.lastname: Peng
authors.forename: Wen-dong
authors.initials: WD
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D015415:Biomarkers; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger