title: Multiple brain tumors of diffuse large B cell lymphoma in a patient with Waldenström's macroglobulinemia/ lymphoplasmacytic lymphoma: PCR and DNA sequence analysis show evidence of differences in clonality of the two B cell malignancies.
abstract: Multiple brain tumors of diffuse large B cell lymphoma (DLBL) were observed in a 75-year-old man with Waldenström's macroglobulinemia (WM). Paravertebral and multiple subcutaneous nodules occurred in succession and he died 4 months after onset. We investigated B cell monoclonality by means of polymerase chain reaction (PCR) analysis and sequencing of the immunoglobulin heavy chain of paraffin-embedded sections. The PCR product of the brain tumors showed a different rearrangement pattern from those of the other sections. The co-occurrence of DLBL with WM is rare, and some investigators have examined the clonality of the two malignancies. This case is important because DLBL brain tumors co-occurred with WM, enabling us to prove that DLBL and WM have different clonality.
authors.affiliation: Department of Neurology, Suwa Red Cross Hospital, Nagano.
authors.forename: Kana
authors.initials: K
authors.lastname: Tojo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hattori
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ito
authors.forename: Naoko
authors.initials: N
authors.lastname: Asano
authors.forename: Kenji
authors.initials: K
authors.lastname: Sano
authors.forename: Takefumi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Keiko
authors.initials: K
authors.lastname: Maruyama
mesh_terms: D000368:Aged; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000150:complications* / Q000235:genetics; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D017809:Fatal Outcome; D019337:Hematologic Neoplasms / Q000150:complications / Q000235:genetics / Q000473:pathology; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000150:complications* / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000150:complications / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000150:complications* / Q000235:genetics; D008279:Magnetic Resonance Imaging; D008297:Male; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013131:Spine / Q000473:pathology; D008258:Waldenstrom Macroglobulinemia / Q000150:complications* / Q000235:genetics