Protein patterning based on electrochemical activation of bioinactive surfaces with hydroquinone-caged biotin.
We report a protein attachment and patterning method based on a hydroquinone-caged biotin surface that generates bioactive biotin by mild electrochemical perturbation. The electrochemical activation proceeds under the buffered aqueous environment at neutral pH. It also allows site-selective generation of bioactive biotin for the immobilization of target protein by using prepatterned electrode arrays.
Kim
Kyuwon
K
Korea Research Institute of Standards and Science, Daejeon 305-600, Korea.
Yang
Haesik
H
Jon
Sangyong
S
Kim
Eunkyung
E
Kwak
Juhyoun
J
D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D004563:Electrochemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006873:Hydroquinones / Q000737:chemistry*; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D001426:Bacterial Proteins; D006873:Hydroquinones; D011506:Proteins; C058865:Strep-avidin conjugated horseradish peroxidase; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase; C031927:hydroquinone