Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries.
A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.
Nielsen
Jeppe L
JL
Department of Civil and Environmental Engineering, University of Washington, 302 More Hall, Box 352700, Seattle, WA 98195-2700, USA.
Schramm
Andreas
A
Bernhard
Anne E
AE
van den Engh
Gerrit J
GJ
Stahl
David A
DA
D041963:Bacteroidetes / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005618:Fresh Water / Q000382:microbiology; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013997:Time Factors
D012336:RNA, Ribosomal, 16S