title: [The kinetics of hematopoietic cell chimerism in the early period after non-myeloablative transplantation and its clinical implications].
abstract: OBJECTIVE
  To analyze the kinetics of hematopoietic cell chimerism in the early period after non-myeloablative stem cell transplantation (NAST) and to investigate the correlation between molecular and hematologic assessment of engraftment or rejection.
  
  
  METHODS
  Short tandem repeat-polymerase chain reaction (STR-PCR) analysis of chimerism status was carried out in 6 patients who received NAST from HLA-matched sibling donors.
  
  
  RESULTS
  In 5/6 patients, the peripheral blood samples collected on the first day after allograft infusion displayed the presence of mixed chimerism. STR-PCR analysis revealed a gradual increase of the donor-specific allelic signal which became dominant over the recipient-specific allele by day +7. On day +14, hematologic chimerisms were completely donor origin. Their molecular engraftments (ME) were detected at a median time of 6 days, preceding hematologic engraftment by a median of 5 days (P > 0.05). But the sixth patient showed more than 50% host residual cells on day +7 and had no signs of ME on day +14.
  
  
  CONCLUSIONS
  It suggested that molecular monitoring of the early dynamics of chimerism after NAST could be useful in predicting engraftment, or rejection. If the engraftment was less than 50% on day +7 and failed to get ME on day +14, the graft rejection would occur.
authors.affiliation: Affiliated Hospital, Academy of Military Medical Science, Beijing 100039, China.
authors.forename: Xiu-bin
authors.initials: XB
authors.lastname: Xiao
authors.forename: Qi-yun
authors.initials: QY
authors.lastname: Sun
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Qiao
authors.forename: Chang-lin
authors.initials: CL
authors.lastname: Yu
authors.forename: Hui-sheng
authors.initials: HS
authors.lastname: Ai
mesh_terms: D000328:Adult; D006084:Graft Rejection; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000209:etiology; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences; D018183:Transplantation Chimera; D014184:Transplantation, Homologous