[Changes and significance of TNF, sIL-2R, IL-6, IL-4 and IFN-gamma levels in plasma from the patients with hemorrhagic fever with renal syndrome].
OBJECTIVE
To explore the changes of TNF, sIL-2R, IL-6, IL-4 and IFN-gamma levels in plasma from patients with hemorrhagic fever with renal syndrome(HFRS) and analyze the correlation between these cytokine levels and alanine aminotransferase(ALT) level in sera from the patients.


METHODS
The cytokine levels were measured by double mAb sandwich ELISA. An automatic biochemical detector(RA-1000) was employed to determine the ALT level.


RESULTS
Levels of TNF, IL-6, IL-4, IFN-gamma and sIL-2R in plasma from the patients were(95.82+/-12.04), (362.46+/-141.26), (17.76+/-3.52), (116.18+/-19.80) ng/L and (898 820+/-127 200) U/L, respectively. These cytokines in plasma from healthy donors were (17.89+/-1.68), (43.81+/-18.08), (4.86+/-1.14), (7.57+/-2.41) ng/L and (66 730+/-29 690) U/L, respectively. The cytokine levels of the patients were notably higher than those of the healthy donors.(P<0.01). At the same time, the average ALT level in sera from the patients was 4.4 fold of that from the healthy donors. Statistical analysis showed that levels of TNF, sIL-2R, IL-6 and IFN-gamma were positively correlated with ALT level, respectively.


CONCLUSIONS
The HFRS patients have a significant increase in both cytokines (TNF, sIL-2R, IL-6 and IFN-gamma) and ALT levels. And there is a positive correlation between those cytokine and ALT levels, suggesting that the liver injury during HTNV infection may be related to the elevation of above cytokine levels.
Liu
Jing-mei
JM
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China. immu_jin@fmmu.edu.cn
Zhu
Yong
Y
Wang
Jiu-ping
JP
Ouyang
Wei-ming
WM
Li
Qi
Q
Zhuang
Ran
R
Jin
Bo-quan
BQ
D000328:Adult; D000410:Alanine Transaminase / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D005260:Female; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D015375:Receptors, Interleukin-2 / Q000097:blood; D048069:Tumor Necrosis Factors / Q000097:blood
D016207:Cytokines; D015850:Interleukin-6; D015375:Receptors, Interleukin-2; D048069:Tumor Necrosis Factors; D015847:Interleukin-4; D007371:Interferon-gamma; D000410:Alanine Transaminase