title: Synthesis of monomers bearing at the 2'-position cyanomethoxycarbonyl group for phosphoramidite oligonucleotide synthesis.
abstract: A novel series of phosphoroamidites for the synthesis of 2-modified oligonucleotides was designed and synthesized on the base of 2'-amino uridine and 2'-amino arabinoadenosine. The amino groups in these compounds were acidified by bis-cyanomethyl esters of different dicarbonic acids. Generated reactive linker groups containing cyanomethoxycarbonyl groups are stable under conditions of oligonucleotide synthesis but could be easily functionalised in post-synthetic stage by treatment with compounds bearing primary amino groups.
authors.affiliation: Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, Russia. vasilieva_2001@ngs.ru
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Vasilyeva
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Abramova
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Silnikov
mesh_terms: D000577:Amides / Q000138:chemical synthesis* / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000138:chemical synthesis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000577:Amides; D009841:Oligonucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid