[The cryopreservation technology in research and development of tissue engineered products].
In this brief review, some key issues related to cryopreservation of seeding cells, scaffolds, and engineered tissues are outlined. The importance of cryopreservation technology to the research and development of tissue engineered products is demonstrated. The biological or biochemical reaction rate must be reduced or completely shut off in order to preserve the tissue engineered products for a long period of time. Cryopreservation may be one of the possible approaches to the fulfillment of this requirement. Seeding cells are stored at low temperature. Tissue engineered scaffold products are usually lyophilized. Engineered tissues are preserved by vitreous cryopreservation technology.
Qin
Tingwu
T
Institute of Reparative and Reconstructive Surgery, West China Hospital of Sichuan University, Chengdu 610041, China.
Yang
Zhiming
Z
D002452:Cell Count; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D023822:Tissue Engineering; D014021:Tissue Preservation; D014022:Tissue Survival