title: Comparison between ultraviolet-visible and near-infrared elastic scattering spectroscopy of chemically induced melanomas in an animal model.
abstract: The work reported compares elastic scattering spectroscopy (ESS) for diagnosis of pigmented skin lesions in two spectral regions: UV-visible and near infrared (NIR). Given the known strong absorption by melanin in the near-UV to mid-visible range of the spectrum, such a comparison can help determine the optimum wavelength range of ESS for diagnosis of pigmented skin lesions. For this purpose, four South American opossums are treated with dimethylbenz(a)anthracene on multiple dorsal sites to induce both malignant melanomas and benign pigmented lesions. Skin lesions are examined in vivo with ESS using both UV-visible and NIR, with wavelength ranges of 330 to 900 nm and 900 to 1700 nm, respectively. Both portable systems use the same fiber optic probe geometry. ESS measurements are made on the lesions, and spectral differences are grouped by diagnosis from standard histopathological procedure. Both ESS datasets show strong spectral trends with the histopathological assignments, and the data suggest a model for the underlying basis of the spectral distinction between benign and malignant pigmented nevi.
authors.affiliation: Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA. oaamar@bu.edu
authors.forename: Ousama M
authors.initials: OM
authors.lastname: A'Amar
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Ley
authors.forename: Irving J
authors.initials: IJ
authors.lastname: Bigio
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D004548:Elasticity; D008545:Melanoma / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D009893:Opossums; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D015127:9,10-Dimethyl-1,2-benzanthracene