title: Initiation of peripheral blood progenitor cell harvest based on peripheral blood hematopoietic progenitor cell counts enumerated by the Sysmex SE9000.
abstract: BACKGROUND
  The most reliable index for timing peripheral blood progenitor cell (PBPC) collection following mobilization is still to be determined. The techniques to enumerate peripheral blood (PB) CD34+ cells are expensive and time-consuming. The SE9000 (Sysmex) provides an estimate of immature cells, called hematopoietic progenitor cells (HPCs). The aim of this study was to prospectively evaluate the efficacy of PB HPC levels for timing PBPC harvest.
  
  
  METHODS
  Thirty-five patients (15 non-Hodgkin's lymphoma and 20 multiple myeloma) were enrolled. PB HPCs and harvested CD34+ cells were counted with the SE9000 and flow cytometry, respectively. Circulating HPCs were monitored daily. PBPC harvest was initiated when HPC levels reached at least 5 per mm(3).
  
  
  RESULTS
  HPC levels reached 5 per mm(3) or more on Median Day 12 (range, days 9 to 16) of mobilizing chemotherapy. The median number of CD34+ cells collected per patient was 19.40 x 10(6) per kg (range, 1.94 x 10(6)-52.55 x 10(6) per kg). Both successful and optimal harvest was achieved in 97 percent of patients. PBPCs were successfully harvested in 25 patients (71%) in one session. An optimal harvest in a single session was attained in 16 patients (46%).
  
  
  CONCLUSIONS
  This might be the first prospective study showing the PB HPC level for timing PBPC harvest.
authors.affiliation: Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. csuh@amc.seoul.kr
authors.forename: Cheolwon
authors.initials: C
authors.lastname: Suh
authors.forename: Shin
authors.initials: S
authors.lastname: Kim
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Jae-Lyun
authors.initials: JL
authors.lastname: Lee
authors.forename: Keon-Uk
authors.initials: KU
authors.lastname: Park
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Jungsin
authors.initials: J
authors.lastname: Lee
authors.forename: Michael W
authors.initials: MW
authors.lastname: Kim
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Chi
authors.forename: Chan Jeong
authors.initials: CJ
authors.lastname: Park
authors.forename: Sang We
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D001772:Blood Cell Count / Q000295:instrumentation*; D002469:Cell Separation / Q000379:methods*; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D011446:Prospective Studies; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34