title: Crucial role for ecto-5'-nucleotidase (CD73) in vascular leakage during hypoxia.
abstract: Extracellular adenosine has been widely implicated in adaptive responses to hypoxia. The generation of extracellular adenosine involves phosphohydrolysis of adenine nucleotide intermediates, and is regulated by the terminal enzymatic step catalyzed by ecto-5'-nucleotidase (CD73). Guided by previous work indicating that hypoxia-induced vascular leakage is, at least in part, controlled by adenosine, we generated mice with a targeted disruption of the third coding exon of Cd73 to test the hypothesis that CD73-generated extracellular adenosine functions in an innate protective pathway for hypoxia-induced vascular leakage. Cd73(-/-) mice bred and gained weight normally, and appeared to have an intact immune system. However, vascular leakage was significantly increased in multiple organs, and after subjection to normobaric hypoxia (8% O(2)), Cd73(-/-) mice manifested fulminant vascular leakage, particularly prevalent in the lung. Histological examination of lungs from hypoxic Cd73(-/-) mice revealed perivascular interstitial edema associated with inflammatory infiltrates surrounding larger pulmonary vessels. Vascular leakage secondary to hypoxia was reversed in part by adenosine receptor agonists or reconstitution with soluble 5'-nucleotidase. Together, our studies identify CD73 as a critical mediator of vascular leakage in vivo.
authors.affiliation: Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 NE 13th St., Oklahoma City, OK 73104, USA. Linda-Thompson@omrf.ouhsc.edu
authors.forename: Linda F
authors.initials: LF
authors.lastname: Thompson
authors.forename: Holger K
authors.initials: HK
authors.lastname: Eltzschig
authors.forename: Juan C
authors.initials: JC
authors.lastname: Ibla
authors.forename: C Justin
authors.initials: CJ
authors.lastname: Van De Wiele
authors.forename: Regina
authors.initials: R
authors.lastname: Resta
authors.forename: Julio C
authors.initials: JC
authors.lastname: Morote-Garcia
authors.forename: Sean P
authors.initials: SP
authors.lastname: Colgan
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D000241:Adenosine / Q000502:physiology; D000818:Animals; D002199:Capillary Permeability; D000860:Hypoxia / Q000378:metabolism*; D008168:Lung / Q000473:pathology; D051379:Mice; D014227:Triazines / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D014227:Triazines; D014230:Triazoles; C097270:ZM 241385; D015720:5'-Nucleotidase; D000241:Adenosine