title: Host-specific encapsidation of a defective RNA 3 of Cucumber mosaic virus.
abstract: Defective (D) RNAs were generated in tobacco upon passage of two isolates of Cucumber mosaic virus (CMV) initially derived from RNA transcripts of cDNA clones. In both cases, the D RNA was derived by a single in-frame deletion of either 339 or 411 nt within the 3a gene of Fny-CMV RNA 3 or M-CMV RNA 3, respectively. The generation of D RNAs was rare and occurred with two CMV isolates, the virions of which were known to differ in physico-chemical properties. The Fny-CMV D RNA 3, designated D RNA 3-1, was maintained by passage together with Fny-CMV in tobacco, but was lost by passage in squash. D RNA 3-1 accumulated in the inoculated squash cotyledons but not in upper, systemically infected leaves. Virions purified from infected squash cotyledons or leaf mesophyll protoplasts did not contain D RNA 3-1. Therefore, the failure of D RNA 3-1 to accumulate in squash leaves systemically infected by CMV was due to a lack of encapsidation of the D RNA 3-1 and movement out of the inoculated leaves.
authors.affiliation: Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Igor B
authors.initials: IB
authors.lastname: Kaplan
authors.affiliation: Department of Biological Sciences, The National University of Singapore, Kent Ridge, Singapore 117543, Singapore.
authors.forename: Kian-Chung
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Tomas
authors.initials: T
authors.lastname: Canto
authors.affiliation: Department of Biological Sciences, The National University of Singapore, Kent Ridge, Singapore 117543, Singapore.|Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Sek-Man
authors.initials: SM
authors.lastname: Wong
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.|Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Palukaitis
mesh_terms: D017799:Cucumovirus / Q000235:genetics*; D028464:Cucurbita / Q000821:virology; D003673:Defective Viruses / Q000235:genetics*; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral / Q000502:physiology*; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*; D019065:Virus Assembly
chemical_list: D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014764:Viral Proteins