Phenotypic characteristics of mouse lung adenoma induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone.
The expression profile of adenoma induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in A/J mice was compared with that of normal lung tissue by suppression subtractive hybridization (SSH). The mRNAs of surfactant-associated protein A (SP-A) and lysozyme showed characteristically higher transcription in the adenoma tissue than in normal lung. High expression of both SP-A and lysozyme in tumor cells was confirmed by in situ hybridization (ISH). In normal lung, alveolar type II pneumocytes were positive for both SP-A and lysozyme, indicating that tumor cells retained the phenotypic characteristics of the murine alveolar type II pneumocytes. Previous studies of human adenocarcinomas have shown that the two proteins are expressed reciprocally; SP-A and lysozyme are differential markers of atypical adenomatous hyperplasia (AAH) and non-goblet cell type adenocarcinoma, and of goblet cell type adenocarcinoma, respectively. Thus, the present results indicate that the phenotype of NNK-induced A/J mouse adenoma differs from that of AAH, which is thought to be a preinvasive lesion of human adenocarcinoma.
Okubo
Chigusa
C
Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki 3058575, Japan.
Morishita
Yukio
Y
Minami
Yuko
Y
Ishiyama
Tadashi
T
Kano
Junko
J
Iijima
Tatsuo
T
Noguchi
Masayuki
M
D000236:Adenoma / Q000139:chemically induced* / Q000473:pathology*; D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D002273:Carcinogens; D018076:DNA, Complementary / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005260:Female; D017403:In Situ Hybridization; D007834:Lasers; D008168:Lung / Q000166:cytology / Q000473:pathology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D009602:Nitrosamines; D010641:Phenotype; D011401:Promoter Regions, Genetic; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism; D013997:Time Factors; D014764:Viral Proteins
D001059:Apoproteins; D002273:Carcinogens; D018076:DNA, Complementary; D009602:Nitrosamines; D037662:Pulmonary Surfactant-Associated Protein A; D012333:RNA, Messenger; D013501:Surface-Active Agents; D014764:Viral Proteins; D012313:RNA; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D009113:Muramidase