Screening flavonoid metabolites of naringin and narirutin in urine after human consumption of grapefruit juice by LC-MS and LC-MS/MS.
The main flavonoids in grapefruit juice, naringin and narirutin, were quantified by LC-MS with structural differentiation by LC-MS/MS. After human consumption of grapefruit juice, urine samples were collected for 24 hours and screened for flavonoid metabolites by LC-MS. The metabolite structures (glucuronides, sulfates, and glucuronide sulfates) were then confirmed via their unique fragmentation patterns by LC-MS/MS. To further verify the identity of the common aglycon (naringenin) shared by the metabolites, enzymatic hydrolysis was performed and the resulting products were analyzed. This work demonstrates that LC-MS and LC-MS/MS techniques can be used for fast metabolite screening without extensive sample preparation.
Zhang
Junmei
J
Department of Chemistry and Biochemistry, University of Texas, 1 University Station A5300, Austin, TX 78712, USA.
Brodbelt
Jennifer S
JS
D000328:Adult; D001628:Beverages; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032083:Citrus paradisi; D004187:Disaccharides / Q000378:metabolism; D005260:Female; D044950:Flavanones / Q000378:metabolism*; D005419:Flavonoids / Q000652:urine*; D006027:Glycosides / Q000652:urine*; D006801:Humans; D008297:Male; D013057:Spectrum Analysis / Q000379:methods
D004187:Disaccharides; D044950:Flavanones; D005419:Flavonoids; D006027:Glycosides; C500601:narirutin; C005274:naringin