title: Isolation of angiotensin converting enzyme (ACE) inhibitory flavonoids from Sedum sarmentosum.
abstract: Bioassay-guided fractionation of the EtOAc-soluble extract of Sedum sarmentosum afforded a new flavonoid, quercetin-3-O-alpha-(6'''-caffeoylglucosyl-beta-1,2-rhamnoside) (1), along with four known flavonoids, quercetin 3-O-alpha-(6'''-p-coumaroylglucosyl-beta-1,2-rhamnoside) (2), isorhamnetin-3-beta-glucopyranoside (3), quercetin-3-beta-glucopyranoside (4), and kaempferol-3-alpha-arabinopyranoside (5). Purification of these compounds was conducted with the application of various chromatographic methods. Compounds 1-5 inhibited angiotensin I converting enzyme (ACE) activity in a concentration-dependent manner. Compounds 1-5 had 50% inhibitory concentration values of 158.9+/-11.1 microgM, 351.6+/-3.9 microgM, 408.9+/-4.6 microgM, 708.8+/-23.1 microgM, and 392.8+/-13.4 microgM.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
authors.forename: Dae-Gill
authors.initials: DG
authors.lastname: Kang
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Kwon
authors.forename: Tae-Oh
authors.initials: TO
authors.lastname: Kwon
authors.forename: Seung-Yeob
authors.initials: SY
authors.lastname: Lee
authors.forename: Duck-Bae
authors.initials: DB
authors.lastname: Lee
authors.forename: Ho-Sub
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D005419:Flavonoids / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D031963:Sedum
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; D007703:Peptidyl-Dipeptidase A