The symbiotic defect in a Sinorhizobium meliloti lipopolysaccharide mutant can be overcome by expression of other surface polysaccharides.
In this work we have examined the extent of functional complementation in symbiosis among different Sinorhizobium meliloti surface polysaccharides including lipopolysaccharide (LPS). We show that a symbiotic deficiency associated with an LPS defect can be reversed by appropriate expression of other surface polysaccharides such as galactoglucan (EPSII) and a particular form of capsular polysaccharide (KdoPS). It is noteworthy that, while succinoglycan EPSI and LPS cannot functionally substitute for each other, they can both be replaced by the same common set of polysaccharides (i.e., EPSII/KdoPS). The complex pattern of functional complementation in symbiosis among S. meliloti surface polysaccharides was shown to be different in Medicago truncatula compared to that previously reported for M. sativa.
Hozbor
Daniela F
DF
Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115, 1900 La Plata, Argentina. hozbor@biol.unlp.edu.ar
Pich Otero
Augusto J L
AJ
Lodeiro
Aníbal R
AR
Del Papa
María Florencia
MF
Pistorio
Mariano
M
Lagares
Antonio
A
D015139:Blotting, Southern; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D008070:Lipopolysaccharides / Q000378:metabolism*; D000455:Medicago sativa / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D009586:Nitrogen Fixation / Q000502:physiology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013559:Symbiosis / Q000502:physiology
D004271:DNA, Fungal; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011994:Recombinant Proteins