title: Fez1 is layer-specifically expressed in the adult mouse neocortex.
abstract: The mammalian cerebral neocortex occupies the largest area of the cerebral cortex and is cytoarchitectually composed of six layers (I-VI). Recent molecular analysis has begun to reveal the existence of various developmental programs, including the genetic regulation of arealization of the neocortex. Although an increasing number of molecular determinants of the developmental stages of the neocortex have been identified, no genes specifically expressed in the adult neocortex have been identified to date. By global screening using microarrays, combined with systematic in situ hybridization, we identified a zinc-finger type transcription factor, Fez1, which is expressed predominantly in the mouse adult neocortex. No other genes in the neocortex have been shown to date to have their expression with such high specificity. Using two-color in situ hybridization, we show that Fez1 is mainly expressed in cortical layers V and VI, not in gamma-aminobutyric acid neurons but in pyramidal neurons, the projection neurons of the cerebral cortex. Immunohistochemistry also shows that Fez1 is expressed in deep layers of the neocortex. Fez1 will be invaluable not only for the molecular understanding of corticogenesis but also for understanding the physiological functions of the adult neocortex, as well as for the use of its promoter in gene-manipulated animals and in conditional expression systems.
authors.affiliation: Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Inoue
authors.forename: Toshio
authors.initials: T
authors.lastname: Terashima
authors.forename: Toru
authors.initials: T
authors.lastname: Nishikawa
authors.forename: Toru
authors.initials: T
authors.lastname: Takumi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008818:Mice, Neurologic Mutants; D019579:Neocortex / Q000033:anatomy & histology / Q000378:metabolism*; D050816:Octamer Transcription Factor-6; D051857:Otx Transcription Factors; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers; D000099260:Empty Spiracles Homeobox Proteins
chemical_list: D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase; D005968:Glutamate Decarboxylase; D018398:Homeodomain Proteins; D007527:Isoenzymes; D050816:Octamer Transcription Factor-6; D051857:Otx Transcription Factors; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D014157:Transcription Factors; D000099260:Empty Spiracles Homeobox Proteins; C494180:Pou3f1 protein, mouse; C402565:glutamate decarboxylase 1