Interleukin-8 in whole blood and clinical status in cystic fibrosis.
Cytokines and polymorphonuclear leukocytes play a key role in immune mediated inflammation in progressive pulmonary damage due to cystic fibrosis. The aim of this study is to establish a simple measure of the host's propensity to secrete inflammatory cytokines and to correlate this with clinical status. Patients (n=44, median age 16 years) with the DeltaF 508 mutation (homozygous) were grouped according to their Shwachman score: Patients with mild disease (Shwachman score 71-100 points, group A, n=22, median FEV(1) 79%) were compared with those with more severe disease (Shwachman score 41-55 points, group B, n=22, median FEV(1) 55%) and age-matched controls (group C, n=22, median FEV(1) 102%). Whole blood was stimulated with 5 ng of lipopolysaccharide (LPS). Interleukin-8 (IL-8) was measured by chemiluminescent immunometric assay (DPC, Bad Nauheim, Germany). Though there was a significant difference at baseline for IL-8 (median group A/B/C 6.1/30.5/5.8 pg/ml; p<0.001), there was no significant difference after stimulation. Moreover, in Pseudomonas aeruginosa positive (Psa+) patients (n=26) there was a significant negative correlation (r=-0.539; p<0.004) between baseline IL-8 and FEV(1) (%). Clinical course and lung function (in Psa+) correlate with IL-8 levels.
Schmitt-Grohé
Sabina
S
Children's Hospital Medical Center, University of Bonn, Adenauerallee 119, 53113 Bonn, Germany. s.schmitt.grohe@uni-bonn.de
Naujoks
Christian
C
Bargon
Joachim
J
Wagner
Thomas O
TO
Schubert
Ralf
R
Hippe
Valerie
V
Zielen
Stefan
S
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000097:blood* / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006720:Homozygote; D006801:Humans; D007118:Immunoassay; D007223:Infant; D007249:Inflammation; D016209:Interleukin-8 / Q000097:blood* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009504:Neutrophils / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D013147:Spirometry
D016207:Cytokines; D016209:Interleukin-8; D008070:Lipopolysaccharides