Host-specific encapsidation of a defective RNA 3 of Cucumber mosaic virus.
Defective (D) RNAs were generated in tobacco upon passage of two isolates of Cucumber mosaic virus (CMV) initially derived from RNA transcripts of cDNA clones. In both cases, the D RNA was derived by a single in-frame deletion of either 339 or 411 nt within the 3a gene of Fny-CMV RNA 3 or M-CMV RNA 3, respectively. The generation of D RNAs was rare and occurred with two CMV isolates, the virions of which were known to differ in physico-chemical properties. The Fny-CMV D RNA 3, designated D RNA 3-1, was maintained by passage together with Fny-CMV in tobacco, but was lost by passage in squash. D RNA 3-1 accumulated in the inoculated squash cotyledons but not in upper, systemically infected leaves. Virions purified from infected squash cotyledons or leaf mesophyll protoplasts did not contain D RNA 3-1. Therefore, the failure of D RNA 3-1 to accumulate in squash leaves systemically infected by CMV was due to a lack of encapsidation of the D RNA 3-1 and movement out of the inoculated leaves.
Kaplan
Igor B
IB
Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
Lee
Kian-Chung
KC
Department of Biological Sciences, The National University of Singapore, Kent Ridge, Singapore 117543, Singapore.
Canto
Tomas
T
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Wong
Sek-Man
SM
Department of Biological Sciences, The National University of Singapore, Kent Ridge, Singapore 117543, Singapore.|Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
Palukaitis
Peter
P
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.|Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
D017799:Cucumovirus / Q000235:genetics*; D028464:Cucurbita / Q000821:virology; D003673:Defective Viruses / Q000235:genetics*; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral / Q000502:physiology*; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*; D019065:Virus Assembly
D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014764:Viral Proteins