DNA interaction with biologically active divalent metal ions: binding constants calculation.
In our previous work we have shown that under the action of Cu2+, Mn2+ and Ca2+ ions DNA is able to transit into a compact state in aqueous solution. In the present work we carried out calculations of binding constants for divalent metal ions interacting with DNA in terms of the macromolecule statistical sum. The formula for calculation of the binding constants and cooperativity parameters was proposed. It was shown that on the "coil state"-"compact (globule) state" transition a single DNA molecule may undergo the first-order phase transition while the transition of the assembly of average DNA chains is of sigmoidal character typical of the cooperative and continuous transition.
Hackl
Elene V
EV
BI Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, 61164 Kharkov, Ukraine. o.hackl@umist.ac.uk
Galkin
Vladimir L
VL
Blagoi
Yurij P
YP
D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005163:Factor Analysis, Statistical; D007477:Ions; D008345:Manganese / Q000737:chemistry / Q000378:metabolism; D008670:Metals / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical
D007477:Ions; D008670:Metals; D008345:Manganese; D003300:Copper; D004247:DNA; D002118:Calcium