[Biological effect of serum of kidney-tonifying traditional Chinese drug-fed rats on cultured osteoblasts].
OBJECTIVE
To investigate the biological effects of the serum of rats fed with kidney-tonifying traditional Chinese drugs on cultured osteoblasts in vitro.


METHODS
The culture media containing the serum from rats fed with the drugs at different doses (high, mediate and low doses) were used to treat the second passage of the osteoblasts from the skull of newborn SD rats, and the cell proliferation, differentiation and mineralization were observed.


RESULTS
The serum stimulated the cell proliferation, enhanced alkaline phosphatase activity and increased the number of mineralized nodules in the cultured osteoblasts.


CONCLUSIONS
Kidney-tonifying traditional Chinese drugs can stimulate the proliferation, differentiation and maturation of cultured osteoblasts in vitro.
Tang
Jing-gang
JG
Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Li
Juan
J
Wu
He-yong
HY
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D012886:Skull / Q000166:cytology
D004365:Drugs, Chinese Herbal; D000469:Alkaline Phosphatase