title: Expression analysis onto microarrays of randomly selected cDNA clones highlights HOXB13 as a marker of human prostate cancer.
abstract: In a strategy aimed at identifying novel markers of human prostate cancer, we performed expression analysis using microarrays of clones randomly selected from a cDNA library prepared from the LNCaP prostate cancer cell line. Comparisons of expression profiles in primary human prostate cancer, adjacent normal prostate tissue, and a selection of other (nonprostate) normal human tissues, led to the identification of a set of clones that were judged as the best candidate markers of normal and/or malignant prostate tissue. DNA sequencing of the selected clones revealed that they included 10 genes that had previously been established as prostate markers: NKX3.1, KLK2, KLK3 (PSA), FOLH1 (PSMA), STEAP2, PSGR, PRAC, RDH11, Prostein and FASN. Following analysis of the expression patterns of all selected and sequenced genes through interrogation of SAGE databases, a further three genes from our clone set, HOXB13, SPON2 and NCAM2, emerged as additional candidate markers of human prostate cancer. Quantitative RT-PCR demonstrated the specificity of expression of HOXB13 in prostate tissue and revealed its ubiquitous expression in a series of 37 primary prostate cancers and 20 normal prostates. These results demonstrate the utility of this expression-microarray approach in hunting for new markers of individual human cancer types.
authors.affiliation: Section of Molecular Carcinogenesis, Male Urological Cancer Research Centre, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK.
authors.forename: S
authors.initials: S
authors.lastname: Edwards
authors.forename: C
authors.initials: C
authors.lastname: Campbell
authors.forename: P
authors.initials: P
authors.lastname: Flohr
authors.forename: J
authors.initials: J
authors.lastname: Shipley
authors.forename: I
authors.initials: I
authors.lastname: Giddings
authors.forename: R
authors.initials: R
authors.lastname: Te-Poele
authors.forename: A
authors.initials: A
authors.lastname: Dodson
authors.forename: C
authors.initials: C
authors.lastname: Foster
authors.forename: J
authors.initials: J
authors.lastname: Clark
authors.forename: S
authors.initials: S
authors.lastname: Jhavar
authors.forename: G
authors.initials: G
authors.lastname: Kovacs
authors.forename: C S
authors.initials: CS
authors.lastname: Cooper
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000032:analysis; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; C484414:HOXB13 protein, human; D018398:Homeodomain Proteins