DNA attachment chemistry at the flexible silicone elastomer surface: toward disposable microarrays.
This paper describes the preparation and surface characterization of maleimide-activated silicone elastomer (PDMS(MCC)) followed by covalent functionalization using thiol-terminated DNA sequences (primary oligo). The stability of this attachment chemistry was demonstrated by the retention of the primary oligo through the process of hybridization with a labeled complementary DNA sequence. In these studies, the hybridized labeled DNA oligomers were detected using confocal fluorescence microscopy. We have employed a vapor deposition technique in which a plasma-treated silicone elastomer (PDMS(OH)) was exposed to vapors of 3-(aminopropyl)triethoxysilane (APTS) under vacuum, to yield the amine-functionalized silicone elastomer (PDMS(NH)(2)). PDMS(NH)(2) was further coupled with a heterofunctional cross-linker, sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate to obtain PDMS(MCC). The surface functionalities of the elastomers were characterized using contact angle measurements and X-ray photoelectron spectroscopy. Surface-modified silicone elastomers appear to be promising substrates for use as substrates for disposable microarrays.
Vaidya
Ashish A
AA
Department of Chemistry, Marshall University, Huntington, West Virginia 25755, USA. vaidya@marshall.edu
Norton
Michael L
ML
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012826:Silicone Elastomers / Q000737:chemistry*; D013499:Surface Properties
D012826:Silicone Elastomers; D004247:DNA