The frequency of alleles of the Pro12Ala polymorphism in PPARgamma2 is different between healthy controls and patients with type 2 diabetes.
The aim of this initial case-control study was to determine the association between common Pro12Ala polymorphism in the PPARgamma2 gene and type 2 diabetes in the Czech Republic. Furthermore, the effect of this polymorphism on phenotypic characteristics and on levels of lipids (total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides) was studied. One hundred thirty-three patients with type 2 diabetes and 97 control subjects were investigated. PCR and RFLP analysis were used for identification of individual genotypes. In the group of patients, three samples (2.26%) were identified as homozygous for the Ala/Ala genotype and 99 samples (74.44%) were homozygotes for the Pro/Pro genotype. Thirty-one samples (23.31%) were identified as Pro12Ala heterozygous. In the control group, six samples (6.19%) were homozygous for the Ala/Ala genotype and 61 samples (62.89%) were homozygotes for the Pro/Pro genotype. Thirty samples (30.93%) were identified as Pro12Ala heterozygous. The allele frequency for the Ala allele was lower in the type 2 diabetic group than in the control group (13.91% vs. 21.43%, P = 0.022). There was no difference (at P < 0.05) between the phenotypic characteristics (BMI, sex) studied in the group of patients according to the Pro12Ala genotype. There was no significant effect of the Pro12Ala polymorphism on lipid levels.
Pintérová
D
D
Division of Cell and Molecular Biology, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic. pinterod@seznam.cz
Cerná
M
M
Kolostová
K
K
Novota
P
P
Cimburová
M
M
Romzová
M
M
Kubena
A
A
Andel
M
M
D000328:Adult; D000368:Aged; D000409:Alanine / Q000235:genetics; D000483:Alleles; D016022:Case-Control Studies; D018153:Czech Republic; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D047495:PPAR gamma / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011392:Proline / Q000235:genetics
D008055:Lipids; D047495:PPAR gamma; D011392:Proline; D000409:Alanine