title: Structure-activity relationships of aminoglycoside-arginine conjugates that bind HIV-1 RNAs as determined by fluorescence and NMR spectroscopy.
abstract: We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dependence on the number and location of arginines in the different aminoglycoside conjugates. The binding affinity of the per-arginine aminoglycosides to TAR is higher than to RRE, and hexa-arginine neomycin B is the most potent binder (KD=5 and 23 nM, respectively). The 2D TOCSY NMR spectrum of the TAR monoarginine-neomycin complex reveals binding at the bulge region of TAR.
authors.affiliation: Department of Organic Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel. aviva.lapidot@weizmann.ac.il
authors.forename: Aviva
authors.initials: A
authors.lastname: Lapidot
authors.forename: Veerappan
authors.initials: V
authors.lastname: Vijayabaskar
authors.forename: Alexander
authors.initials: A
authors.lastname: Litovchick
authors.forename: Jingua
authors.initials: J
authors.lastname: Yu
authors.forename: Thomas L
authors.initials: TL
authors.lastname: James
mesh_terms: D000617:Aminoglycosides / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001120:Arginine / Q000737:chemistry; D001665:Binding Sites; D005454:Fluorescence Polarization; D015497:HIV-1 / Q000235:genetics*; D015394:Molecular Structure; D009355:Neomycin / Q000737:chemistry / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010303:Paromomycin / Q000737:chemistry / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012367:RNA, Viral; D010303:Paromomycin; D001120:Arginine; D009355:Neomycin