title: Ochratoxin A-induced apoptosis in rat kidney tissue.
abstract: The aim of our study was to find whether ochratoxin A (OTA) induces the apoptosis and/or necrosis of kidney tissue in rats. In the first experiment, the highest number of apoptotic cells was found in rats sacrificed one day after OTA administration (1.00 mg/kg b.w., i.p.). The number of apoptotic cells reduced gradually and they were not seen nine days after OTA administration. A possible dose-dependence of histological changes was checked in kidney tissue of rats given 0.25, 0.50 or 1.00 mg of OTA/kg b.w., i.p. three times a week for four weeks. The number of apoptotic cells showed a clear dose-dependence, but necrosis was absent even at the highest doses. The time-dependent appearance of lesions related to OTA administration was checked by administering 0.50 mg OTA/kg body weight to rats, and sacrificing them one day after 1, 3, 6, and 9 doses/administrations, or 6 and 21 day after 12 doses/administrations. Long-term administration is associated with continued and increased apoptosis without necrosis, suggestive of OTA's role in the pathogenesis of progressive renal atrophy.
authors.affiliation: Unit of Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
authors.forename: Ana-Marija
authors.initials: AM
authors.lastname: Domijan
authors.forename: Maja
authors.initials: M
authors.lastname: Peraica
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Ferencić
authors.forename: Snjezana
authors.initials: S
authors.lastname: Cuzić
authors.forename: Radovan
authors.initials: R
authors.lastname: Fuchs
authors.forename: Ana
authors.initials: A
authors.lastname: Lucić
authors.forename: Bozica
authors.initials: B
authors.lastname: Radić
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002273:Carcinogens / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D009793:Ochratoxins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002273:Carcinogens; D009793:Ochratoxins; C025589:ochratoxin A