Killing of periodontopathogenic bacteria by photodynamic therapy.
BACKGROUND
The aim of this study was to evaluate a new approach for killing periodontopathogenic bacteria using photodynamic therapy (PDT).


METHODS
In this study, we investigated the photosensitizers chlorin e6, BLC 1010, and BLC 1014 by three different methods for their effect in PDT on the viability of periodontopathogenic bacterial species. The methods included examination of inhibition zones on agar plates, determination of colony-forming units (CFU), and the use of a bacterial viability kit.


RESULTS
Using the CFU method, we were able to demonstrate that the anaerobic bacteria Porphyromonas gingivalis, Fusobacterium nucleatum, and Capnocytophaga gingivalis can be photoinactivated completely by illumination with an intensity of 5.3 J/cm2 in the presence of 10 microM chlorin e6 and 10 microM BLC 1010. With the photosensitizers chlorin e6 and BLC 1010, we were able to induce zones of inhibition on agar plates. BLC 1014 failed to produce a zone of inhibition. The results of the bacterial viability test also showed that the photosensitizer BLC 1014 provides the lowest photodynamic effect in comparison to the others.


CONCLUSIONS
The data collected to date suggest that photodynamic therapy with chlorin e6 and BLC 1010 is advantageous for suppressing periodontopathogenic bacteria.
Pfitzner
Anne
A
Department of Conservative Dentistry, Friedrich Schiller University of Jena, Jena, Germany.
Sigusch
Bernd W
BW
Albrecht
Volker
V
Glockmann
Eike
E
D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D002206:Capnocytophaga / Q000187:drug effects; D002735:Chlorophyllides; D015169:Colony Count, Microbial; D016124:Eikenella corrodens / Q000187:drug effects; D016967:Fusobacterium nucleatum / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006801:Humans; D007834:Lasers; D010510:Periodontal Diseases / Q000382:microbiology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology; D011166:Porphyrins / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000187:drug effects; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology
D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C062985:phytochlorin