title: Photo-polymerized microarchitectural constructs prepared by microstereolithography (muSL) using liquid acrylate-end-capped trimethylene carbonate-based prepolymers.
abstract: Precision microarchitectural constructs made of acrylated trimethylene carbonate (TMC)-based liquid prepolymers were photo-polymerized using a custom-designed microstereolithographic apparatus. In this study, three different photo-polymerizable liquid prepolymers were prepared by the polymerization of TMC with a low molecular weight poly(ethylene glycol) (PEG) (mol. wt. 200 or 1000); designated as PEG200 or PEG1000, respectively or trimethylolpropane (TMP) as an initiator, and subsequently end-capped with an acrylate group. As a result of layer-by-layer photo-irradiation of the prepolymer with a movable ultraviolet light pen driven by computer-aided design, a three-dimensional (3D) micropillar array, a microbank array, a microcone array, and multi-microtunnels formed on a platform plate or a glass plate were precisely fabricated. The PEG-based polymers exhibited a very low cell adhesion potential, whereas the TMP-based hydrophobic polymer exhibited high cell adhesion and proliferation potentials. The microbank array, which consisted of a plate made of the TMP-based polymer and microbanks made of the PEG200-based polymer, caused cell adhesion and proliferation only on the plate. Upon the implantation of microcone arrays under the subcutis of rats, the photo-polymerized construct made of the poorly swellable PEG200-based polymer exhibited only surface erosion and limited drug loading and releasing potentials. On the other hand, the photo-polymerized construct made of the highly swellable PEG1000-based polymer exhibited not only surface erosion but also bulk erosion and high drug loading and releasing potentials. In this paper, we discuss their potential biomedical applications.
authors.affiliation: Division of Biomedical Engineering, Graduate School of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D016475:3T3 Cells; D000179:Acrylates / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D003692:Delayed-Action Preparations / Q000737:chemistry; D003907:Dexamethasone / Q000008:administration & dosage; D004146:Dioxanes / Q000737:chemistry*; D005549:Foreign-Body Reaction / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D020007:Implants, Experimental / Q000009:adverse effects; D008422:Materials Testing; D051379:Mice; D008832:Microchemistry / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D010777:Photochemistry / Q000379:methods*; D010781:Photography / Q000379:methods; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000179:Acrylates; D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004146:Dioxanes; D011108:Polymers; D012996:Solutions; C443643:trimethylene carbonate; D003907:Dexamethasone