title: Uncovering novel cell cycle players through the inactivation of securin in budding yeast.
abstract: Budding yeast securin/Pds1p, an inhibitor of the anaphase activator separase/Esp1p, is involved in several checkpoint pathways and in promoting Esp1p's nuclear localization. Using a modified synthetic genetic array (SGA) screen for genes that become essential in the absence of Pds1p, we uncovered roles for uncharacterized genes in cell cycle processes, including Esp1p activation.
authors.affiliation: The Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0840, USA.
authors.forename: Sumeet
authors.initials: S
authors.lastname: Sarin
authors.forename: Karen E
authors.initials: KE
authors.lastname: Ross
authors.forename: Lorrie
authors.initials: L
authors.lastname: Boucher
authors.forename: Yvette
authors.initials: Y
authors.lastname: Green
authors.forename: Mike
authors.initials: M
authors.lastname: Tyers
authors.forename: Orna
authors.initials: O
authors.lastname: Cohen-Fix
mesh_terms: D002453:Cell Cycle / Q000235:genetics* / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D005804:Genes, Lethal; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D004718:Saccharomycetales / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D064249:Securin
chemical_list: D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C578004:PDS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D064249:Securin