title: Secretoneurin in the human aqueous humor and the absence of an effect of frequently used eye drops on the levels.
abstract: Secretoneurin (SN) was detected in the aqueous humor (AH) of patients treated topically with tobramycine eye drops alone or tobramycine and cyclosporine A, tobramycine and diclofenac or tobramycine and rimexolone. The levels of the peptide were found to be higher in the uninflamed human than in the rabbit aqueous humor which may be the result of species differences and/or age-related circumstances. Furthermore, they are approximately one hundred times higher than those of classical neuropeptides indicating release from nerve fibers and/or secretion from non-pigmented ciliary epithelium cells. Despite a slight tendency by rimexolone to decrease the levels, there was no significant effect seen for either of the drops. It must be considered that aminoglycosides are known to have toxic side effects and that they can influence the levels of SN which may be not diminished by low topical doses of corticosteroids or nonsteroidal antiinflammatory drugs. The high levels of the peptide are of relevance and may indicate a significant role of secretoneurin in the anterior segment of the eye. This should encourage performing functional studies.
authors.affiliation: Clinical Department of Ophthalmology, Innsbruck Medical University, Anichstrasse 35, 6020 Innsbruck, Austria.
authors.forename: Katrin
authors.initials: K
authors.lastname: Stemberger
authors.forename: Julia
authors.initials: J
authors.lastname: Pallhuber
authors.forename: Alfred
authors.initials: A
authors.lastname: Doblinger
authors.forename: Josef
authors.initials: J
authors.lastname: Troger
authors.forename: Rudolf
authors.initials: R
authors.lastname: Kirchmair
authors.forename: Martina
authors.initials: M
authors.lastname: Kralinger
authors.forename: Reiner
authors.initials: R
authors.lastname: Fischer-Colbrie
authors.forename: Gerhard
authors.initials: G
authors.lastname: Kieselbach
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000737:chemistry* / Q000187:drug effects; D016572:Cyclosporine / Q000627:therapeutic use; D004008:Diclofenac / Q000627:therapeutic use; D004338:Drug Combinations; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009479:Neuropeptides / Q000032:analysis*; D009883:Ophthalmic Solutions / Q000627:therapeutic use; D011245:Pregnadienes / Q000627:therapeutic use; D053381:Secretogranin II; D014031:Tobramycin / Q000627:therapeutic use; D014606:Uveitis, Anterior / Q000188:drug therapy*
chemical_list: D004338:Drug Combinations; D009479:Neuropeptides; D009883:Ophthalmic Solutions; D011245:Pregnadienes; D053381:Secretogranin II; D004008:Diclofenac; C081159:secretoneurin; D016572:Cyclosporine; C013769:rimexolone; D014031:Tobramycin