Impact of persistent cytomegalovirus infection on human neuroblastoma cell gene expression.
In a model of human neuroblastoma (NB) cell lines persistently infected with human cytomegalovirus (HCMV) we previously showed that persistent HCMV infection is associated with an increased malignant phenotype, enhanced drug resistance, and invasive properties. To gain insights into the mechanisms of increased malignancy we analyzed the global changes in cellular gene expression induced by persistent HCMV infection of human neuroblastoma cells by use of high-density oligonucleotide microarrays (HG-U133A, Affymetrix) and RT-PCR. Comparing the gene expression of different NB cell lines with persistently infected cell sub-lines revealed 11 host cell genes regulated in a similar manner throughout all infected samples. Nine of these 11 genes may contribute to the previously observed changes in malignant phenotype of persistently HCMV infected NB cells by influencing invasive growth, apoptosis, angiogenesis, and proliferation. Thus, this work provides the basis for further functional studies.
Hoever
Gerold
G
Center of Hygiene, Institute of Medical Virology, J. W. Goethe-University Hospital, Paul-Ehrlich Str. 40, 60596 Frankfurt am Main, Germany.
Vogel
Jens-Uwe
JU
Lukashenko
Polina
P
Hofmann
Wolf-Karsten
WK
Komor
Martina
M
Doerr
Hans Wilhelm
HW
Cinatl
Jindrich
J
D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics* / Q000821:virology*; D015854:Up-Regulation / Q000235:genetics