title: Leptin fluctuates in intestinal ischemia-reperfusion injury as inflammatory cytokine.
abstract: As leptin is an active mediator mainly secreted by adipose tissue and is closely related with energy metabolism, we evaluate both the changes of leptin levels in serum and adipose tissue with a concise radioimmunoassay and the changes of leptin mRNA expression in adipose tissue with RT-PCR, during the severe metabolic impediment in rat intestinal ischemia-reperfusion (I/R) injury. Results show that not only leptin levels in serum and adipose tissue but also its mRNA expression in adipose tissue undergo a fluctuation according to different injury times. Therefore, we conclude that leptin has a time-dependent response to acute inflammatory stimuli and acts as an anti-inflammatory cytokine.
authors.affiliation: Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Lin
authors.forename: Guang-Tao
authors.initials: GT
authors.lastname: Yan
authors.forename: Lu-Huan
authors.initials: LH
authors.lastname: Wang
authors.forename: Xiu-Hua
authors.initials: XH
authors.lastname: Hao
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents; D001483:Base Sequence; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism; D007249:Inflammation; D007422:Intestines / Q000098:blood supply*; D020738:Leptin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D020738:Leptin; D012333:RNA, Messenger