Comparison of the mouse antibody production (MAP) assay and polymerase chain reaction (PCR) assays for the detection of viral contaminants.
Mouse antibody production (MAP) tests have become the standard assay for the detection of murine viral contamination in biologic materials, such as cell lines and transplantable tumors. However, newly developed PCR assays offer the advantage of lower cost, faster turn around times, and eliminate the use of live animals. In this study, the MAP test and a panel of PCR assays were compared for the detection of 11 different viral contaminants of cell lines and transplantable tumors. The PCR assays had either better or comparable results to the MAP test for all agents tested. The results of this study confirm that PCR assays are an effective method for detection of viral contamination and can be used as an alternative to the MAP test.
Bauer
Beth A
BA
Research Animal Diagnostic Laboratory, Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA. bauerbe@missouri.edu
Besch-Williford
Cynthia L
CL
Riley
Lela K
LK
D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D002478:Cells, Cultured; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004866:Equipment Contamination; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
D004279:DNA, Viral; D012367:RNA, Viral