title: Evaluation of recombinant antigens for the measurement of antibodies to streptolysin O and streptococcal DNase B on the Beckman Coulter IMMAGE system and comparison with a method employing conventional antigens.
abstract: The performance of a newly devised method employing recombinant antigens for the determination of antibodies to streptolysin O and DNase B on the Beckman Coulter IMMAGE System was compared with a nephelometric assay using conventional antigens. Sera from 114 patients with or without evidence of streptococcal infection were analysed by the two techniques. The results obtained with the new method correlated well with those of the reference test. The sensitivity of a combination measurement of antibodies to streptolysin O and DNase B was higher than that obtained when measuring each parameter alone. Handling of the tests was easier on the Beckman Coulter IMMAGE System. In conclusion, the new test from Beckman Coulter for the measurement of antibodies to streptolysin O and DNase B performs well and equals measurements using conventional antigens on a nephelometer.
authors.affiliation: Institute of Medical Microbiology & National Reference Center for Streptococci, University Hospital, 52057 Aachen, Germany. BFreyaldenhoven@ukaachen.de
authors.forename: Bettina Sigrid
authors.initials: BS
authors.lastname: Freyaldenhoven
authors.forename: Gabriele
authors.initials: G
authors.lastname: Moos
authors.forename: Rolf Dieter
authors.initials: RD
authors.lastname: Hinzmann
authors.forename: Rudolf
authors.initials: R
authors.lastname: Lütticken
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D003851:Deoxyribonucleases / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D009391:Nephelometry and Turbidimetry; D012372:ROC Curve; D011994:Recombinant Proteins / Q000276:immunology; D012044:Regression Analysis; D015203:Reproducibility of Results; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000276:immunology*; D013301:Streptolysins / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D013301:Streptolysins; C025964:streptolysin O; D003851:Deoxyribonucleases; C020069:deoxyribonuclease B