title: Effect of NADH dehydrogenase-disruption and over-expression on respiration-related metabolism in Corynebacterium glutamicum KY9714.
abstract: The function of type II NADH dehydrogenase (NDH-2) in Gram-positive Corynebacterium glutamicum was investigated by preparing strains with ndh, the NDH-2 gene, disrupted and over-expressed. Although disruption showed no growth defects on glucose minimum medium, the growth rate of the over-expressed strain was lower compared with its parent, C. glutamicum KY9714. Ndh-disruption and over-expression did not lead to a large change in the respiratory chain and energetics, including the cytochrome components and the H(+)/O ratio. However, in the strain that lacked NDH-2, membrane L-lactate oxidase activity increased, while NDH-2 over-expression led to decreased L-lactate and malate oxidase activities. In addition, relatively high cytoplasmic lactate dehydrogenase (LDH) activity was always present as was malate dehydrogenase, irrespective of NDH-2 level. Furthermore, L-lactate or malate-dependent NADH oxidase activity could be reproduced by reconstitution with the membranes and the cytoplasmic fraction isolated from the disruptant. These results suggest that coupling of LDH and the membrane L-lactate oxidase system, together with the malate-dependent NADH oxidase system, operates to oxidize NADH when the NDH-2 function is defective in C. glutamicum.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8511, Japan.
authors.forename: Nawarat
authors.initials: N
authors.lastname: Nantapong
authors.forename: Youko
authors.initials: Y
authors.lastname: Kugimiya
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
mesh_terms: D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D009154:Mutation; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D010101:Oxygen Consumption
chemical_list: D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D009245:NADH Dehydrogenase