title: PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity.
abstract: Pst1p was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. ECM33 encodes a protein homologous to Pst1p, and both of them display typical features of GPI-anchored proteins and a characteristic receptor L-domain. Pst1p and Ecm33p are both localized to the cell surface, Pst1p being at the cell membrane and possibly also in the periplasmic space. Here, the characterization of pst1Delta, ecm33Delta and pst1Delta ecm33Delta mutants is described. Deletion of ECM33 leads to a weakened cell wall, and this defect is further aggravated by simultaneous deletion of PST1. As a result, the ecm33Delta mutant displays increased levels of activated Slt2p, the MAP kinase of the cell integrity pathway, and relies on a functional Slt2-mediated cell integrity pathway to ensure viability. Analyses of model glycosylated proteins show glycosylation defects in the ecm33Delta mutant. Ecm33p is also important for proper cell wall ultrastructure organization and, furthermore, for the correct assembly of the mannoprotein outer layer of the cell wall. Pst1p seems to act in the compensatory mechanism activated upon cell wall damage and, in these conditions, may partially substitute for Ecm33p.
authors.affiliation: Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Pardo
authors.forename: Lucía
authors.initials: L
authors.lastname: Monteoliva
authors.forename: Paloma
authors.initials: P
authors.lastname: Vázquez
authors.forename: Raquel
authors.initials: R
authors.lastname: Martínez
authors.forename: Gloria
authors.initials: G
authors.lastname: Molero
authors.forename: César
authors.initials: C
authors.lastname: Nombela
authors.forename: Concha
authors.initials: C
authors.lastname: Gil
mesh_terms: D002473:Cell Wall / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C497117:ECM33 protein, S cerevisiae; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; C499369:PST1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors