title: Isolation and characterization of two positional isomers of novel heterogeneous branched cyclomaltohexaoses (alpha-cyclodextrins) having a D-galactobiosyl residue on the side chain.
abstract: Transgalactosylated products, 2-O-alpha-D-galactobiosyl-cyclomaltohexaoses (alpha-cyclodextrins, alphaCDs), were synthesized by alpha-galactosidase from coffee bean using melibiose and alphaCD as a donor substrate and an acceptor, respectively. Two positional isomers of 2-O-alpha-galactobiosyl-alphaCDs were isolated and purified by HPLC, and their structures were elucidated by FABMS and NMR spectroscopies, as well as by an enzymatic degradation method. The chromatographic behavior of these novel galactosylated alphaCDs was compared on three HPLC columns with different separation modes.
authors.affiliation: School of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya 663-8179, Japan. okada@mwu.mukogawa-u.ac.jp
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Okada
authors.forename: Masanori
authors.initials: M
authors.lastname: Semma
authors.forename: Sumio
authors.initials: S
authors.lastname: Kitahata
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ichikawa
mesh_terms: D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D040503:Coffea / Q000737:chemistry*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D008553:Melibiose / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D047391:alpha-Cyclodextrins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D047391:alpha-Cyclodextrins; D008553:Melibiose; D000519:alpha-Galactosidase