title: Expression of a beta-defensin mRNA, lingual antimicrobial peptide, in bovine mammary epithelial tissue is induced by mastitis.
abstract: The expression of a beta-defensin, the lingual antimicrobial peptide (LAP), in response to mastitis was investigated by real-time PCR of RNA from mastitic and control udder quarters. There was a positive relationship between somatic cell count in milk and LAP expression. In situ hybridization showed that LAP mRNA was expressed in epithelial cells of mastitic tissue. These results suggest that LAP plays a role in the innate immune response to mastitis.
authors.affiliation: Dairy Biotechnology, AgResearch, Hamilton, New Zealand. kara.swanson@agresearch.co.nz
authors.forename: Kara
authors.initials: K
authors.lastname: Swanson
authors.forename: Stas
authors.initials: S
authors.lastname: Gorodetsky
authors.forename: Laura
authors.initials: L
authors.lastname: Good
authors.forename: Stephen
authors.initials: S
authors.lastname: Davis
authors.forename: David
authors.initials: D
authors.lastname: Musgrave
authors.forename: Kerst
authors.initials: K
authors.lastname: Stelwagen
authors.forename: Vicki
authors.initials: V
authors.lastname: Farr
authors.forename: Adrian
authors.initials: A
authors.lastname: Molenaar
mesh_terms: D000818:Animals; D002417:Cattle; D002452:Cell Count; D005260:Female; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D023083:beta-Defensins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D023083:beta-Defensins