title: Y chromosome micro-deletions in idiopathic infertility from Northern India.
abstract: Azoospermia factor locus (AZF) is assumed to contain the genes responsible for spermatogenesis. Deletions in these genes are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An attempt was made to establish the prevalence of micro-deletions on the Y chromosome in 79 infertile North Indians with azoospermia and oligozoospermia. Detail clinical examinations as well as endocrinological parameters were also done. Polymerase chain reaction (PCR) micro-deletion analysis was done in 79 infertile men. For this, genomic DNA was extracted from the peripheral blood. Seven sets of primers were used encompassing AZFa, AZFb and AZFc regions. Micro-deletions in five of the 79 cases (6.3%) showed deletions of at least one of the STS markers. Deletions were detected with known and unknown aetiology and at least in one of the infertile male with varicocele. AZF micro-deletions seen in idiopathic infertile males suggest the need for molecular screening in non-idiopathic cases.
authors.affiliation: Department of Urology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, India. rmittal@sgpgi.ac.in <rmittal@sgpgi.ac.in>
authors.forename: Rama Devi
authors.initials: RD
authors.lastname: Mittal
authors.forename: Gunjana
authors.initials: G
authors.lastname: Singh
authors.forename: Aneesh
authors.initials: A
authors.lastname: Srivastava
authors.forename: Mandakini
authors.initials: M
authors.lastname: Pradhan
authors.forename: Akanchha
authors.initials: A
authors.lastname: Kesari
authors.forename: Annu
authors.initials: A
authors.lastname: Makker
authors.forename: Balraj
authors.initials: B
authors.lastname: Mittal
mesh_terms: D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004247:DNA / Q000235:genetics; D056426:Genetic Loci; D006801:Humans; D007194:India; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D029589:Seminal Plasma Proteins / Q000235:genetics; D012729:Sex Chromosome Aberrations
chemical_list: D029589:Seminal Plasma Proteins; D004247:DNA