title: [Application of PSD-MALDI-TOF-MS to protein identification].
abstract: OBJECTIVE
  To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.
  
  
  METHODS
  The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.
  
  
  RESULTS
  Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.
  
  
  CONCLUSIONS
  PSD technique has greater application prospects in peoteomics.
authors.affiliation: National Center of Biomedical Analysis, Beijing 100850, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Song-cheng
authors.initials: SC
authors.lastname: Yang
authors.forename: Sheng-ming
authors.initials: SM
authors.lastname: Wu
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Kai-hua
authors.initials: KH
authors.lastname: Wei
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000737:chemistry*; D000595:Amino Acid Sequence; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D012269:Ribosomal Proteins / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D010959:Tissue Plasminogen Activator / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D000324:Adrenocorticotropic Hormone; D010959:Tissue Plasminogen Activator