title: Optimization of 13C direct detection NMR methods.
abstract: (13)C-detected experiments are still limited by their inherently lower sensitivity, as compared to the equivalent (1)H-detected experiments. Improving the sensitivity of (13)C detection methods remains a significant area of NMR research that may provide better means for studying large macromolecular systems by NMR. In this communication, we show that (13)C-detected experiments are less sensitive to the salt concentration of the sample solution than (1)H-detected experiments. In addition, acquisition can be started with anti-phase coherence, resulting in higher sensitivity due to the elimination of the final INEPT transfer step.
authors.affiliation: University of California San Francisco, San Francisco, California 94143, USA.
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Shimba
authors.forename: Helena
authors.initials: H
authors.lastname: Kovacs
authors.forename: Alan S
authors.initials: AS
authors.lastname: Stern
authors.forename: Anson M
authors.initials: AM
authors.lastname: Nomura
authors.forename: Ichio
authors.initials: I
authors.lastname: Shimada
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Hoch
authors.forename: Charles S
authors.initials: CS
authors.lastname: Craik
authors.forename: Volker
authors.initials: V
authors.lastname: Dötsch
mesh_terms: D002247:Carbon Isotopes; D007930:Leucine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000494:pharmacology; D012996:Solutions / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D012996:Solutions; D012965:Sodium Chloride; D007930:Leucine