Induction of PR-1 accumulation accompanied by runaway cell death in the lsd1 mutant of Arabidopsis is dependent on glutathione levels but independent of the redox state of glutathione.
The lesions simulating disease (lsd) mutants of Arabidopsis spontaneously develop hypersensitive-response-like lesions in the absence of pathogens. To address the function of the redox regulator glutathione in disease resistance, we examined the relationship between endogenous glutathione and PR-1 accumulation using one of these mutants, lsd1, as a disease resistance model. Lesion formation on lsd1 was suppressed by weak light and initiated by the subsequent transition to normal light. The application of buthionine sulfoximine, a specific inhibitor of glutathione biosynthesis, suppressed conditionally induced runaway cell death and expression of the PR-1 gene, suggesting that glutathione regulates the conditional cell death and PR-1 gene expression. The application of reduced (GSH) or oxidized (GSSG) glutathione to lsd1 upregulated the level of total glutathione ([GSH]+[GSSG]) accompanied by hastened accumulation of PR-1, and the basal level of total glutathione in lsd1 was higher than that in wild-type plants. The glutathione redox state defined as [GSH]/([GSH]+[GSSG]) decreased following the conditional transition, but the suppression of this decrease by the application of GSH did not inhibit the accumulation of PR-1. Taken together, conditional PR-1 accumulation in lsd1 is regulated not by the redox state but by the endogenous level of glutathione.
Senda
Kaori
K
Research Institute for Biological Sciences, Okayama (RIBS Okayama), 7549-1 Yoshikawa, Kayou-cho, Okayama, 716-1241 Japan.
Ogawa
Ken'ichi
K
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D016923:Cell Death; D004268:DNA-Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000494:pharmacology; D010084:Oxidation-Reduction; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C104920:LSD1 protein, Arabidopsis; D014157:Transcription Factors; C077262:PR-1 protein, Arabidopsis; D019328:Buthionine Sulfoximine; D005978:Glutathione; D019803:Glutathione Disulfide