title: PKCdelta is involved in signal attenuation in CD3+ T cells.
abstract: PKCdelta has been implicated in the signalling events after engagement of the antigen specific receptor on B cells and the Fc-epsilon receptor on mast cells. Employing our recently established PKCdelta null mice , we here investigate the physiological function of PKCdelta in CD3+ T cells. As result, administration of anti-CD3 antibodies in vivo induced markedly increased blood plasma IL-2 levels (but not IL-4, IFN-gamma, TNF-alpha and IL-6 levels) in the PKCdelta null mice, when compared to wild-type sibling controls. Additionally, in vitro T cell proliferative responses to allogenic MHC are significantly enhanced in PKCdelta-deficient CD3+ T cells. These findings suggest that PKCdelta serves a so far unrecognized role in TCR-induced negative regulation of IL-2 cytokine production and T cell proliferation.
authors.affiliation: Department of Medical Biology and Human Genetics, Innsbruck Medical University, Schoepfstrasse 41, A-6020 Innsbruck, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gruber
authors.forename: Johannes
authors.initials: J
authors.lastname: Barsig
authors.forename: Christa
authors.initials: C
authors.lastname: Pfeifhofer
authors.forename: Nassim
authors.initials: N
authors.lastname: Ghaffari-Tabrizi
authors.forename: Inge
authors.initials: I
authors.lastname: Tinhofer
authors.forename: Michael
authors.initials: M
authors.lastname: Leitges
authors.forename: Gottfried
authors.initials: G
authors.lastname: Baier
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D017252:CD3 Complex / Q000032:analysis* / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000378:metabolism; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D011493:Protein Kinase C / Q000235:genetics / Q000502:physiology*; D051745:Protein Kinase C-delta; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology*
chemical_list: D000906:Antibodies; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4; C497259:Prkcd protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta