[Studies on sex identification and variation of endogenous hormones in female and male plants of Gynostemma pentaphyllum].
OBJECTIVE
To distinguish female and male plants of Gynostemma pentaphyllum quickly and accurately in the stage of seedling and to understand the relation between sex differentiation and endogenous plant hormone.


METHODS
Stained methods of BTB and enzyme linked immuno-sorbent Assay were used.


RESULTS
10 Hours after being dyed, the liquid extract of female plants turned into yellow, while extract of male ones turned green. There were obvious differences on iPAs and GA(1 + 3) contents but no differences on IAA and ABA contents between male and female plants during the stages of seedling and vegetative growth.


CONCLUSIONS
BTB can be used for distinguishing female and male plants and there were a certain relation between sex and endogenous plant hormones.
Wang
Qing-ya
QY
College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. qingyawang@yahoo.com.cn
Guo
Qiao-sheng
QS
Sun
Jian-yun
JY
Zhang
Shou-dong
SD
Li
Yang-han
YH
D005875:Gibberellins / Q000378:metabolism*; D029774:Gynostemma / Q000254:growth & development / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism*; D012737:Sex Factors
D005875:Gibberellins; D010937:Plant Growth Regulators