Induction of a homeodomain-leucine zipper gene by auxin is inhibited by cytokinin in Arabidopsis roots.
Homeobox genes are essential regulators of the development of plants as well as other organisms. We chose eight putative Arabidopsis homeobox genes not previously characterized and examined their expression in response to treatment with auxin/cytokinin. One of them, ATHB53, was further studied because it was auxin-inducible and its induction was inhibited by cytokinin. Its full-length cDNA was cloned and found to encode a protein of the HD-Zip superfamily. Whole-mount in situ hybridization and RT-PCR showed that it was expressed in the root meristem, and auxin treatment increased its expression, especially in a region from 0.3 to 0.6mm from the root tip. These results suggest that ATHB53 plays a regulatory role in auxin/cytokinin signaling during root development.
Son
Ora
O
Department of Biological Science, Sookmyung Women's University, Seoul, Republic of Korea.
Cho
Hee-Yeon
HY
Kim
Mi-Ran
MR
Lee
Hyosoo
H
Lee
Myeong-Sok
MS
Song
Eunsook
E
Park
Jong Hoon
JH
Nam
Kyung Hee
KH
Chun
Jong-Yoon
JY
Kim
Ho-Jung
HJ
Hong
Soon-Kwan
SK
Chung
Yong-Yoon
YY
Hur
Cheol-Goo
CG
Cho
Hyung-Taeg
HT
Cheon
Choong-Ill
CI
D017360:Arabidopsis / Q000187:drug effects* / Q000378:metabolism*; D001483:Base Sequence; D003583:Cytokinins / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018398:Homeodomain Proteins / Q000737:chemistry / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D016350:Leucine Zippers / Q000502:physiology; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism*
D003583:Cytokinins; D018398:Homeodomain Proteins; D007210:Indoleacetic Acids