title: [Determination of luteolin-7-O-glycoside in the herb of Dracocephalum rupestra by HPLC].
abstract: OBJECTIVE
  To develop a method for determination of luteolin-7-O-glycoside in the roots, stems, leafs, flowers and aerial parts of Dracocephalum rupestra sampled in different seasons.
  
  
  METHODS
  The samples were analyzed on an phenomenex C18 column, with mobile phase of methanol-acetonitrile-0.4% phosphoric acid (30:8:62) at flow rate 1.0 mL x min(-1) and detection at wavelength of 350 nm.
  
  
  RESULTS
  The content of luteolin-7-O-glycoside in different parts of D. rupestra was different maximum in leaves, while minimum in stems. Luteolin-7-O-glycoside in D. rupestra sampled before blossoming was the highest.
  
  
  CONCLUSIONS
  The method simple, accurate and suitable for the quality evaluation of this plant medicine.
authors.affiliation: School of Pharmacy, Shandong University, Jinan 250012, China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Ren
authors.forename: Hong-xiang
authors.initials: HX
authors.lastname: Lou
authors.forename: Bin
authors.initials: B
authors.lastname: Ma
authors.forename: Mei
authors.initials: M
authors.lastname: Ji
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006027:Glycosides / Q000032:analysis*; D019686:Lamiaceae / Q000737:chemistry*; D047311:Luteolin / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012621:Seasons
chemical_list: D006027:Glycosides; D047311:Luteolin