Ability of human CDC25B phosphatase splice variants to replace the function of the fission yeast Cdc25 cell cycle regulator.
CDC25 phosphatases are essential and evolutionary-conserved actors of the eukaryotic cell cycle control. To examine and compare the properties of three splicing variants of human CDC25B, recombinant fission yeast strains expressing the human proteins in place of the endogenous Cdc25 were generated and characterized. We report, that the three CDC25B variants: (i) efficiently replace the yeast counterpart in vegetative growth, (ii) partly restore the gamma and UV radiation DNA damage-activated checkpoint, (iii) fail to restore the DNA replication checkpoint activated by hydroxyurea. Although these yeast strains do not reveal the specific functions of the human CDC25B variants, they should provide useful screening tools for the identification of new cell cycle regulators and pharmacological inhibitors of CDC25 phosphatase.
Lemaire
Matthieu
M
Institut d'Exploration Fonctionnelle des Génomes, Université Paul Sabatier, LBCMCP-CNRS UMR5088, IFR109 118 route de Narbonne, 31077 Toulouse, France.
Mondesert
Odile
O
Bugler
Béatrix
B
Ducommun
Bernard
B
D017398:Alternative Splicing; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004249:DNA Damage / Q000502:physiology; D004261:DNA Replication / Q000502:physiology; D005656:Fungal Proteins / Q000502:physiology*; D005720:Gamma Rays; D005800:Genes, Fungal / Q000502:physiology; D018816:Genes, cdc / Q000502:physiology; D006801:Humans; D007527:Isoenzymes; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D014466:Ultraviolet Rays; D020687:cdc25 Phosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020666:ras-GRF1 / Q000502:physiology*
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D007527:Isoenzymes; D011994:Recombinant Proteins; D020666:ras-GRF1; C517008:CDC25B protein, human; D020687:cdc25 Phosphatases