title: Functional interaction in establishment of ribosomal integrity between small subunit protein rpS6 and translational regulator rpL10/Grc5p.
abstract: Functional ribosomes synthesize proteins in all living cells and are composed of two labile associated subunits, which are made of rRNA and ribosomal proteins. The rRNA of the small 40S subunit (SSU) of the functional eukaryotic 80S ribosome decodes the mRNA molecule and the large 60S subunit (LSU) rRNA catalyzes protein synthesis. Recent fine structure determinations of the ribosome renewed interest in the role of ribosomal proteins in modulation of the core ribosomal functions. RpL10/Grc5p is a component of the LSU and is a multifunctional translational regulator, operating in 60S subunit biogenesis, 60S subunit export and 60S subunit joining with the 40S subunit. Here, we report that rpL10/Grc5p functionally interacts with the nuclear export factor Nmd3p in modulation of the cellular polysome complement and with the small subunit protein rpS6 in subunit joining and differential protein expression.
authors.affiliation: Department of Cell Biology, Paris Lodron University Salzburg, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Pachler
authors.forename: Thomas
authors.initials: T
authors.lastname: Karl
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kolmann
authors.forename: Norbert
authors.initials: N
authors.lastname: Mehlmer
authors.forename: Michaela
authors.initials: M
authors.lastname: Eder
authors.forename: Michael
authors.initials: M
authors.lastname: Loeffler
authors.forename: Kamil
authors.initials: K
authors.lastname: Oender
authors.forename: Elisabeth O
authors.initials: EO
authors.lastname: Hochleitner
authors.forename: Friedrich
authors.initials: F
authors.lastname: Lottspeich
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Bresgen
authors.forename: Klaus
authors.initials: K
authors.lastname: Richter
authors.forename: Michael
authors.initials: M
authors.lastname: Breitenbach
authors.forename: Lore
authors.initials: L
authors.lastname: Koller
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D000080882:Ribosomal Protein L10; D038601:Ribosomal Protein S6 / Q000235:genetics / Q000502:physiology*; D012269:Ribosomal Proteins / Q000235:genetics / Q000502:physiology*; D012270:Ribosomes / Q000235:genetics / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015003:Yeasts / Q000235:genetics / Q000502:physiology*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins