title: Freezing of human immature oocytes using cryoloops with Taxol in the vitrification solution.
abstract: OBJECTIVE
  In human frozen immature oocytes, there has been little successful delivery. We examined the feasibility of vitrification solution including Taxol, cytoskeltal stabilizer.
  
  
  METHODS
  We set four experimental groups that immature oocytes has cumulus cells or not, or including Taxol or not in the vitrification solution. Frozen-thawed oocytes have been performed IVM, ICSI, and IVC.
  
  
  RESULTS
  There were no significant differences in survival, maturation, and fertilization rate, respectively. However, in the group enveloped by cumulus cells and including Taxol in the vitrification solution, one embryo was developed to blastocyst.
  
  
  CONCLUSIONS
  Our results showed that using vitrification solution with Taxol proved so effective.
authors.affiliation: Department of Gynecology and Urology, Ladies Clinic Kyono, Japan. lckyono-3@yacht.ocn.ne.jp
authors.forename: Kohei
authors.initials: K
authors.lastname: Fuchinoue
authors.forename: Noritaka
authors.initials: N
authors.lastname: Fukunaga
authors.forename: Setsuyo
authors.initials: S
authors.lastname: Chiba
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakajo
authors.forename: Akiko
authors.initials: A
authors.lastname: Yagi
authors.forename: Koichi
authors.initials: K
authors.lastname: Kyono
mesh_terms: D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology*; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted
chemical_list: D003451:Cryoprotective Agents; D017239:Paclitaxel