[Application of random amplification polymorphic DNA in the genotyping of Neisseria gonorrhoeae].
OBJECTIVE
To set up random amplified polymorphic DNAs (RAPD) method in genotyping Neisseria gonorrhoeae on DNA level, and to explore its use to trace the source of infection.


METHODS
Four different pretreatments were used to extract the Neisseria gonorrhoeae genomic DNA with its advantages and disadvantages compared. Arbitrary sequence was then used to amplify the genomic DNA of Neisseria gonorrhoeae and RAPD fingerprint maps was applied to distinct the Neisseria gonorrhoeae strains. Finally, RAPD fingerprint of Neisseria gonorrhoeae strain between patient and his/her sexual partner was compared.


RESULTS
Cetyltrimethylammonium bromide method was classical in extracting genomic DNA, and could get integrated genomic DNA and good fingerprint maps, since main segments were common to all the Neisseria gonorrhoeae but some were different among strains so that the fingerprint of different Neisseria gonorrhoeae were distinctive. However, fingerprint maps of Neisseria gonorrhoeae collected from sex partners were quite similar.


CONCLUSIONS
Based on genomic levels, effective fingerprint maps could be identified and to classify the Neisseria gonorrhoeae into different genotypes. RAPD fingerprint maps could be used to trace the source of infection.
Zhang
Tie-jun
TJ
School of Public Health, Fudan University, Shanghai 200032, China.
Ren
Yan-hua
YH
Zhang
Ying-hua
YH
Zhou
Xiao-ming
XM
Yu
Shun-zhang
SZ
Jiang
Qing-wu
QW
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial