[Determination of luteolin-7-O-glycoside in the herb of Dracocephalum rupestra by HPLC].
OBJECTIVE
To develop a method for determination of luteolin-7-O-glycoside in the roots, stems, leafs, flowers and aerial parts of Dracocephalum rupestra sampled in different seasons.


METHODS
The samples were analyzed on an phenomenex C18 column, with mobile phase of methanol-acetonitrile-0.4% phosphoric acid (30:8:62) at flow rate 1.0 mL x min(-1) and detection at wavelength of 350 nm.


RESULTS
The content of luteolin-7-O-glycoside in different parts of D. rupestra was different maximum in leaves, while minimum in stems. Luteolin-7-O-glycoside in D. rupestra sampled before blossoming was the highest.


CONCLUSIONS
The method simple, accurate and suitable for the quality evaluation of this plant medicine.
Ren
Dong-mei
DM
School of Pharmacy, Shandong University, Jinan 250012, China.
Lou
Hong-xiang
HX
Ma
Bin
B
Ji
Mei
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006027:Glycosides / Q000032:analysis*; D019686:Lamiaceae / Q000737:chemistry*; D047311:Luteolin / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012621:Seasons
D006027:Glycosides; D047311:Luteolin