[Allele frequencies and species specificity of six short tandem repeat loci in Chinese population].
OBJECTIVE
To develop a set of new markers for forensic application, the authors have chosen 6 short tandem repeat(STR) loci to study the allele frequencies and species specificity in Chinese Han population in Chengdu.


METHODS
One hundred and ten EDTA-blood samples were collected from the unrelated individuals in Chengdu city, Sichuan province. DNA was extracted by Chelex-100 and amplified by the polymerase chain reaction(PCR). Polyacrylamide gel electrophoresis (PAGE) and silver staining were used to analyze the PCR products.


RESULTS
The polymorphisms of all 6 STR loci have been obtained in Chinese Han population in Chengdu, the alleles of D4S2366, D4S2367, D6S474, D6S1281, D2S1396 and D20S601 being 7, 7, 6, 7, 5, 7, the observed heterozygosity of them being 0.802, 0.708, 0.770, 0.627, 0.542, 0.672, the discrimination power of them being 0.887, 0.828, 0.849, 0.848, 0.794, 0.865; and the power of exclusion of them being 0.602, 0.441, 0.544, 0.325, 0.227, 0.386. Evaluated by comparison with the data from 14 different animals as controls, the 6 STR loci contain good specificity of human beings.


CONCLUSIONS
The 6 STR loci are highly polymorphic and can play a key role in species identification. They are new candidate markers for forensic personal identification and paternity testing.
Zhang
Wei-juan
WJ
Department of Forensic Biology, College of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P. R. China.
Hou
Yi-ping
YP
Wu
Jin
J
Jia
Zhen-jun
ZJ
Zhou
Xue-ping
XP
Li
Ying-bi
YB
Tian
Jing
J
Yan
Jing
J
D000818:Animals; D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005554:Forensic Medicine; D005787:Gene Frequency; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D011110:Polymorphism, Genetic; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics*