title: Photoanalogues of the initiation substrates of the RNA polymerase II, 5-azido-2-nitrobenzoyl derivatives of the ATP gamma-amidophosphate: the possible photoinduced degradation of the functional group to an N-arylhydroxylamine.
abstract: Photoanlogues of the initiation substrates of the RNA polymerase II, N3ArNH(CH2)(n)NHpppA where N3Ar is 5-azido-2-nitrobenzoyl group (n = 2 or 4) were synthesized, allowing the preparation of photoreactive oligonucleotides in situ by RNA polymerase II for application as photolabels. Photolysis of p-nitro-substituted aromatic azide in aqueous medium was investigated. Using the azoxy-coupling reaction it was possible to determine whether a nitrene or p-nitrophenyl hydroxylamine azoxy compound is the trappable intermediate that is generated at ambient temperature in aqueous solution.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Popova
authors.forename: V S
authors.initials: VS
authors.lastname: Mal'shakova
authors.forename: P V
authors.initials: PV
authors.lastname: Alekseyev
authors.forename: N V
authors.initials: NV
authors.lastname: Kudryashova
authors.forename: M M
authors.initials: MM
authors.lastname: Shakirov
authors.forename: L L
authors.initials: LL
authors.lastname: Savinkova
authors.forename: I A
authors.initials: IA
authors.lastname: Drachkova
authors.forename: T S
authors.initials: TS
authors.lastname: Godovikova
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry*; D001345:Autoradiography; D001386:Azides / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006898:Hydroxylamines / Q000737:chemistry*; D010777:Photochemistry; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001386:Azides; D006898:Hydroxylamines; D000255:Adenosine Triphosphate; D012319:RNA Polymerase II