[Effect of radix angelicae sinensis decoction for supplementing blood on inhibiting the increase of endothelial cell monolayer permeability induced by hypoosmotic solution].
OBJECTIVE
To study effect of radix Angelicae sinensis decoction for supplementing blood (RASDSB) on inhibiting the increase of endothelial cell monolayer permeability induced by hypoosmotic solution.


METHODS
The endothelial cells isolated from newborn bovine aorta were cultured on polycarbonate microporous filter membrane to develop compact endothelial monolayer. Fluid filtration coefficient (Kf), filtration volume (Jv) and osmotic reflective coefficient (sigma) to protein of the endothelial monolayer were measured treated by hypoosmotic solution (changing concentration of serum in M199 solution from 20% to 2%) for 120 min or by hypoosmotic solution containing 10(-4) g x ml(-1) RASDSB for 120 min after perfused Hanks balanced salt solution containing 5 g x L(-1) albumin.


RESULTS
Kf and Jv of the endothelial monolayer treated by hypoosmotic solution increased and sigma of that decreased. RASDSB could inhibit above-mentioned change. Morphological analysis demonstrated that RASDSB could inhibit widening of intercellular distance and enlargement of cellular area in the endothelial monolayer induced by hypoosmolality.


CONCLUSIONS
Hypoosmotic solution could increase endothelial cell monolayer permeability and RASDSB could inhibit the increase.
Fan
Y
Y
Institute of Radiological Medicine, Shanghai Medical University, Shanghai 200032.
Wu
D
D
Gong
Y
Y
Hu
Z
Z
Sheng
M
M
D029971:Angelica sinensis; D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D019587:Dietary Supplements; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000737:chemistry / Q000187:drug effects*; D009997:Osmotic Pressure / Q000187:drug effects
D004365:Drugs, Chinese Herbal; C044613:angelicae sinensis extract