Hypoxanthine effects on cyclic AMP levels in human lymphocytes.
We have measured hypoxanthine effect on cAMP levels in PBL in basal conditions (no agonist), and with the addition of 2-(p-[2-carboxyethyl] phenylethylamino)-5'-N-ethylcarboxamidoadenosine (CGS-21680, a specific A2 receptor agonist). We have found that hypoxanthine, at 25 microM and 50 microM concentrations, increases cAMP levels in PBL in basal and A2 agonist stimulated conditions.
Torres
R J
RJ
Biochemistry Laboratory, La Paz University Hospital, Madrid, Spain.
DeAntonio
I
I
Prior
C
C
Puig
J G
JG
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000959:Antihypertensive Agents / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019271:Hypoxanthine / Q000097:blood / Q000494:pharmacology*; D007926:Lesch-Nyhan Syndrome / Q000097:blood; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010627:Phenethylamines / Q000494:pharmacology; D043683:Receptors, Adenosine A2 / Q000378:metabolism; D018047:Receptors, Purinergic P1 / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D000959:Antihypertensive Agents; D010627:Phenethylamines; D043683:Receptors, Adenosine A2; D018047:Receptors, Purinergic P1; C061282:2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine; D019271:Hypoxanthine; D000242:Cyclic AMP; D000241:Adenosine