Age-related molecular polymorphism of the heterodimeric proteoglycan Bisdermican.
Bisdermican (PG760) is a large, heterodimeric, dermatan sulfate proteoglycan found in selected basement membranes, smooth muscle cell layers, and different extracellular matrices. Age-dependent and developmentally regulated alterations in glycosaminoglycan structure and quantity have been shown to be functionally relevant for a number of physiological and pathological processes. Bisdermican was purified from human skin fibroblast cultures of different age and confluency. Following beta-elimination, glycosaminoglycan chains were analyzed by Sephacryl-S-300 chromatography. Glycosaminoglycan chains of Bisdermican from infantile fibroblasts had a molecular weight of 19 kDa, whereas the glycosaminoglycan chain of the large Bisdermican subunit purified from confluent fetal fibroblast secretions was slightly larger (Mr = 24 kDa). Bisdermican derived from subconfluent cultures of fetal fibroblasts displayed the largest glycosaminoglycan chains with a molecular weight of 31.5 kDa for the large subunit, and a molecular weight of 22 kDa for the small subunit. Thus, Bisdermican displays a molecular polymorphism that is related to its chronological age and proliferative state.
Götte
Martin
M
Departments of Obstetrics and Gynecology, Münster University Hospital, Münster, Germany. mgotte@uni-muenster.de
Sofeu Feugaing
David D
DD
Kresse
Hans
H
D000375:Aging / Q000502:physiology*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005333:Fetus; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D007223:Infant; D008970:Molecular Weight; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification; D012867:Skin
D011509:Proteoglycans; C508424:bisdermican, human