title: [The influence of local changes in the temperature-dependent conformational mobility of thioredoxins on their thermostability].
abstract: For the development of a method for the prediction of single point mutations substantially affecting protein thermostability, we studied the effect of the E85R and R82E mutations on the thermostability of thioredoxins from Escherichia coli (Trx) and Bacillus acidocaldarius (BacTrx), respectively. The basic method of investigation was the molecular dynamics simulation of 3D protein models in a particular solvent at different temperatures (300 and 373 K). Some thermolabile regions in Trx, BacTrx, and their mutants were revealed by analyzing the temperature effect on the molecular dynamics of the protein molecule. The effect of single point mutations on the temperature changes of the protein conformation mobility in several thermolabile regions was found. The results of the calculations are in accord with the experimental data indicating that the mutation E85R increases Trx thermostability, whereas the mutation R82E decreases BacTrx thermostability. The thermostability of these proteins was revealed to depend on ionic interactions between the thermolabile regions. The single point mutations change the parameters of these interactions and make them more favorable in the E85R-Trx mutant and less favorable in the R82E-BacTrx mutant. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // www.maik.ru.
authors.forename: A A
authors.initials: AA
authors.lastname: Polianskiĭ
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Kosinskiĭ
authors.forename: R G
authors.initials: RG
authors.lastname: Efremov
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D006860:Hydrogen Bonding; D007477:Ions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017354:Point Mutation; D011487:Protein Conformation; D013696:Temperature; D013879:Thioredoxins / Q000737:chemistry* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D007477:Ions; C491202:trxC protein, E coli; D013879:Thioredoxins