[Protective effects of phenolic alkaloids from Menispermum dauricum on inflammatory injury following focal cerebral ischemia-reperfusion in rats].
OBJECTIVE
To study the protective effects of phenolic alkaloids from Menispermum dauricum (PAMd) on inflammatory injury following focal cerebral ischemia-reperfusion in rats.


METHODS
The right middle cerebral artery of the rat was occluded by inserting a nylon suture through the internal carotid artery for 2 h, followed by reperfusion by withdrawing the suture. The expression of intercellular adhesion molecule-1 (ICAM-1) was observed by immunohistochemistry staining. The adhesiveness and infiltration of leucocytes were observed by HE staining. The activity of myeloperoxidase (MPO) and the content of nitric oxide (NO) in the cortex and hippocampus were measured.


RESULTS
PAMd was shown to markedly inhibit ICAM-1 expression, alleviate the adhesiveness and infiltration of leucocytes, and decrease the MPO activity and the NO content in ischemic cortex and hippocampus.


CONCLUSIONS
PAMd has protective effects on inflammatory injury following focal cerebral ischemia-reperfusion by inhibiting ICAM-1 expression, alleviating the adhesiveness and infiltration of leucocytes and decreasing the generation of NO.
Zhang
Xiao-juan
XJ
Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Guo
Lian-jun
LJ
Qu
Ling
L
Lü
Qing
Q
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D044182:Benzylisoquinolines / Q000302:isolation & purification / Q000494:pharmacology*; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007962:Leukocytes / Q000473:pathology; D008297:Male; D031603:Menispermum / Q000737:chemistry; D018696:Neuroprotective Agents / Q000494:pharmacology; D020556:Neutrophil Infiltration / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D044005:Tetrahydroisoquinolines / Q000302:isolation & purification / Q000494:pharmacology*
D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D044182:Benzylisoquinolines; D018696:Neuroprotective Agents; D044005:Tetrahydroisoquinolines; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide; C023968:daurisoline; C035934:dauricine; D009195:Peroxidase