Engineering switches, genetically.
Ostermeier, Guntas, and Mitchel describe a new approach to design enzymes that are allosterically regulated by an unrelated ligand . The resulting protein, constructed by nonhomologous recombination and genetic screens, displays switch-like behavior.
Roberts
Richard W
RW
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
D001665:Binding Sites; D005812:Genes, Switch / Q000235:genetics; D008320:Maltose / Q000378:metabolism*; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011995:Recombination, Genetic; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D008320:Maltose; D001618:beta-Lactamases