title: [Expression and DNA binding activity of NF-kappaB in mucosal tissue of chronic rhinosinusitis].
abstract: OBJECTIVE
  To study the expression and activation of nuclear factor-kappa B (NF-kappaB) in mucosa of rhinosinusitis.
  
  
  METHODS
  The expressions of NF-kappaB p50 and p65 in the mucosa from 18 patients and 10 norms were detected with the method of immunohistochemistry. The activation of DNA-binding proteins which was labelled with 32P-radiolabeled oligonucleotide probe for NF-kappaB was detected with electrophoretic mobility shift assays (EMSA) in mucosa.
  
  
  RESULTS
  Plasma of epithelial and glandular cells displayed a strongly positive staining reaction to p65, and nucleus displayed a strongly positive staining reaction to p50 (7.8%-52.1%). There was significantly difference (chi2 = 22.917, P < 0.01). The DNA-binding proteins activity of 18 samples from patients with chronic rhinosinusitis (28.14 +/- 16.71) was stronger than that (9.28 +/- 2.84) in normal subjects (t = 4.56, P < 0.05).
  
  
  CONCLUSIONS
  The expression and DNA-binding proteins activity of NF-kappaB was enhanced. It indicated that NF-kappaB was activated in mucosal inflammation of chronic rhinosinusitis.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital, Sun Yet-sen University, Guangzhou 510630, China.
authors.forename: Tong-li
authors.initials: TL
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Ge-hua
authors.initials: GH
authors.lastname: Zhang
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Sun
authors.forename: Yong-qi
authors.initials: YQ
authors.lastname: Li
mesh_terms: D016022:Case-Control Studies; D002908:Chronic Disease; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*
chemical_list: D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C498143:RELA protein, human; D051996:Transcription Factor RelA; D004247:DNA