Differential effects of CD28 costimulation upon cytokine production by CD4+ and CD8+ T cells.
The impact of CD28 ligation upon CD4+ and CD8+ T lymphocyte proliferation and cytokine production was assessed. Although costimulation increased the proliferative response of both T cell subsets, cytokine production was most markedly increased in the CD4+ subset, as evidenced by a 40-fold increase in interleukin-2 (IL-2), a 14-fold increase in interleukin-3 (IL-3) and 5-fold increases in interferon gamma and GM-colony-stimulating factor (CSF) production. The CD8+ T cell response to CD28 ligation was less marked, maxima being a 5-fold increase in IL-2 production and 2-fold increases in IL-3 and GM-CSF production. Resolution of CD4+ and CD8+ T cells into their CD44lo (naïve) and CD44hi (memory/effector) subsets revealed that naive CD4+ T cells were the most CD28-responsive subsets. CD28-mediated costimulation promotes distinct differentiation programs in CD4+ versus CD8+ T cells.
Rochford
Rosemary
R
Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse University, Syracuse, NY 13210, USA.
Riggs
James E
JE
Clavo
Anaira
A
Ernst
David N
DN
Hobbs
Monte V
MV
D000818:Animals; D018106:CD28 Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D018106:CD28 Antigens; D016207:Cytokines; D018960:Hyaluronan Receptors; D012333:RNA, Messenger