title: Molecular diversity and accelerated evolution of C-type lectin-like proteins from snake venom.
abstract: A number of C-type lectin-like proteins that affect thrombosis and hemostasis by inhibiting or activating specific platelet membrane receptors or blood coagulation factors have been isolated from the venom of various snake species and characterized and more than 80 have been sequenced. Recent data on the primary sequences and 3D structures of C-type lectins and C-type lectin-like proteins from snake venoms have enabled us to analyze their molecular evolution. Statistical analysis of their cDNA sequences shows that C-type lectin-like proteins, with some exceptions, have evolved in an accelerated manner to acquire their diverse functions. Phylogenetic analysis shows that the A and B chains of C-type lectin-like proteins are clearly separated from C-type lectins and that the A and B chains are further divided into a group of platelet receptor-binding proteins and a group of coagulation factor-binding proteins. Elucidation of the tertiary structures of several C-type lectin-like proteins led to the discovery of a unique domain-swapping interaction between heterodimeric subunits, which creates a concave surface for ligand binding.
authors.affiliation: Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University, Sendai 981-8555, Japan. ogawa@biochem.tohoku.ac.jp
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.forename: Takahito
authors.initials: T
authors.lastname: Chijiwa
authors.forename: Naoko
authors.initials: N
authors.lastname: Oda-Ueda
authors.forename: Motonori
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D019143:Evolution, Molecular; D037181:Lectins, C-Type / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010974:Platelet Aggregation / Q000187:drug effects; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D012910:Snake Venoms / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D012911:Snakes
chemical_list: D000925:Anticoagulants; D037181:Lectins, C-Type; D012910:Snake Venoms