title: Hydride generation interface for speciation analysis coupling capillary electrophoresis to inductively coupled plasma mass spectrometry.
abstract: A novel hydride generation (HG) interface for coupling capillary electrophoresis (CE) with inductively coupled plasma mass spectrometry (ICPMS) is presented in this work. The CE-HG-ICPMS interface was applied to the separation and quantitation of common arsenic species. Lack of a commercially available HG interface for CE-ICPMS led to a three concentric tube design allowing alleviation of back pressure commonly observed in CE-HG-ICPMS. Due to the high sensitivity and element-specific detection of ICPMS, quantitative analysis of As(III), As(V), monomethylarsonic acid, and dimethylarsinic acid was achieved. Optimization of CE separation conditions resulted in the use of 20 mmol L(-1) sodium borate with 2% osmotic flow modifier (pH 9.0) and -20 kV applied potential for baseline resolution of each arsenic species in the shortest time. Hydride generation conditions were optimized through multiple electrophoretic separation analyses with 5% HCl and 3% NaBH(4) (in 0.2% NaOH) determined to be the optimum conditions. After completion of system optimization, detection limits obtained for the arsenic species were less than 40 ng L(-1) with electromigration time precision less than 1% within a total analysis time of 9.0 min. Finally, the interface was used for speciation analysis of arsenic in river and tap water samples.
authors.affiliation: Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221-0172, USA.
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Richardson
authors.forename: Sasi S
authors.initials: SS
authors.lastname: Kannamkumarath
authors.forename: Rodolfo G
authors.initials: RG
authors.lastname: Wuilloud
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Caruso
mesh_terms: D001151:Arsenic / Q000032:analysis*; D001152:Arsenicals / Q000032:analysis*; D001894:Borohydrides / Q000737:chemistry*; D002101:Cacodylic Acid / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006851:Hydrochloric Acid / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D001152:Arsenicals; D001894:Borohydrides; C025364:sodium borohydride; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; D001151:Arsenic; D006851:Hydrochloric Acid