Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells.
This chapter describes the use of microscope-based fluorescence resonance energy transfer to follow dynamic interaction of molecules localized at focal adhesions. We first outline the significance of studying dynamic interactions in focal adhesions of living cells, and second provide an overview of the method itself. This is followed by a protocol for fluorescence resonance energy transfer measurements in live cells.
Ballestrem
Christoph
C
Department of Moelcular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel.
Geiger
Benjamin
B
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002468:Cell Physiological Phenomena; D002477:Cells / Q000166:cytology*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics
D008164:Luminescent Proteins