title: [Genetic analysis of a heading-delayed mutant by T-DNA insertion in rice (Oryza sativa L.)].
abstract: Transposon tagging was used to isolate genes in higher plant. In this study, a delayed heading mutant caused by T-DNA insertion in rice was identified. Genetic analysis of the mutant showed that the three types of phenotype, normal heading, delayed heading and overly delayed heading in the segregating populations derived from the T-DNA heterozygotes fit the ratio of 1:2:1. Test for Basta resistance showed the delayed heading plants were all resistant while the normal heading plants were susceptible, and the ratio of resistant and susceptible plants was 3:1, which indicated that the delayed heading mutant was co-segregated with Basta resistance. The delayed heading mutant caused by T-DNA insertion was confirmed by T-DNA detection using PCR method. This delayed heading mutant will be used for isolation of the tagged gene in rice.
authors.affiliation: Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhao-Gui
authors.initials: ZG
authors.lastname: Chen
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.forename: Ze-Min
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Zhu
authors.forename: Xin-Shan
authors.initials: XS
authors.lastname: Wan
authors.forename: Jing-Liu
authors.initials: JL
authors.lastname: Zhang
authors.forename: Gui-Quan
authors.initials: GQ
authors.lastname: Zhang
mesh_terms: D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA