title: Genotyping SNPs with the LightCycler.
abstract: Here, a single nucleotide polymorphism typing methodology is described based on polymerase chain reaction monitoring, in real time, of fluorescently labeled amplified products using the LightCycler. The main advantages of the system are the time required for the analysis (about 20 min), combined with the robustness, accuracy, and the sensitivity of the method.
authors.affiliation: Institute of Legal Medicine, Faculty of Medicine, University of Santiago de Compostela, Santiago de Compostela, Galicia, Spain.
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Lareu
authors.forename: Clara
authors.initials: C
authors.lastname: Ruiz-Ponte
mesh_terms: D001483:Base Sequence; D041322:Chromosomes, Human, Y; D017931:DNA Primers; D005456:Fluorescent Dyes; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes