title: An unusually high level of quinolone resistance associated with type II topoisomerase mutations in quinolone resistance-determining regions of Aeromonas caviae isolated from diarrhoeal patients.
abstract: We examined 158 strains belonging to different Aeromonas species isolated from hospitalized acute diarrhoea cases for susceptibility to quinolones. Compared to other species, a high percentage of the A. caviae strains were resistant to ciprofloxacin and norfloxacin. Based on MIC values, 6 A. caviae strains were selected and the nucleotide sequences for the quinolone-resistant-determining regions (QRDRs) of gyrA, gyrB and parC genes were analysed. In resistant strains, double mutations (Ser(83)-->Ile and Asp(87)-->Asn) and a single mutation (Ser(80)-->Ile) were detected in the QRDR of gyrA and parC, respectively.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata 700 010, India.
authors.forename: Sutapa
authors.initials: S
authors.lastname: Sinha
authors.forename: S
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: S K
authors.initials: SK
authors.lastname: Bhattacharya
authors.forename: G B
authors.initials: GB
authors.lastname: Nair
authors.forename: T
authors.initials: T
authors.lastname: Ramamurthy
mesh_terms: D000333:Aeromonas / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009643:Norfloxacin / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D002939:Ciprofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase; D009643:Norfloxacin