Bifidobacterium longum as an oral delivery system of endostatin for gene therapy on solid liver cancer.
To overcome difficulties that hampered widespread application of a specific delivery system in cancer gene therapy and to inhibit the growth of solid liver cancer, we utilized a strain of Bifidobacterium longum as a delivery system to transport an endostatin gene that can inhibit growth of tumor. The B. longum strain with the endostatin gene (B. longum-En) was taken orally by tumor-bearing nude mice through drencher preparation. The results showed that B. longum-En could strongly inhibit the growth of solid liver tumor in nude mice and prolong the survival time of tumor-bearing nude mice. Furthermore, tumor growth was inhibited more efficiently when the B. longum-En treatment included selenium. Enriching the B. longum-En treatment with selenium improves the activity of NK and T cells and stimulates the activity of IL-2 and TNF-alpha in BALB/c mice. These results suggest that B. longum may be a highly specific and efficient vector for transporting anticancer genes in cancer gene therapy.
Fu
Geng-Feng
GF
Medical School, Nanjing University, Nanjing, China.
Li
Xi
X
Hou
Ya-Yi
YY
Fan
Yan-Rong
YR
Liu
Wen-Hua
WH
Xu
Gen-Xing
GX
D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D016503:Drug Delivery Systems; D043169:Endostatins / Q000008:administration & dosage*; D015316:Genetic Therapy; D005822:Genetic Vectors; D007376:Interleukin-2 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000382:microbiology / Q000628:therapy*; D008223:Lymphoma / Q000235:genetics / Q000382:microbiology / Q000628:therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012643:Selenium / Q000627:therapeutic use; D015996:Survival Rate; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D043169:Endostatins; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D012643:Selenium