Effects of gibberellin mutations on tolerance to apical meristem damage in Arabidopsis thaliana.
To examine the role of gibberellin hormones (GAs) in tolerance to apical meristem damage (AMD), we characterized the reaction norms of several GA-deficient and insensitive mutants of Arabidopsis thaliana in response to AMD and compared them to those of the wild type, Landsberg, from which they were derived. We included 'natural' genotypes of A. thaliana--accessions with shorter lab histories--in order to evaluate how representative Landsberg is of other genotypes. The GA mutations did not alter the level of tolerance to AMD, which was consistent with equal compensation for all genotypes. Generally, the reaction norms to AMD did not differ among the GA mutants themselves, or between the GA mutants and Landsberg. The GA mutations did affect the overall phenotypes of the plants, but these effects were not simply related to whether the mutation was early or late in the biochemical pathways. The GA-insensitive mutant was phenotypically different from the GA-deficient mutants and from Landsberg. The natural populations differed significantly from Landsberg, particularly in attributes related to size and inflorescence production, one more example of the need for researchers to be careful when generalizing the results of studies based upon laboratory strains. Our results indicate that early-flowering genotypes of A. thaliana can be remarkably tolerant to AMD, and that GA deficiency/insensitivity does not hinder tolerance to AMD, at least in this genetic background. Moreover, we confirm that mutations at regulatory loci can have noncatastrophic effects on fitness, as recently found by other investigators.
Banta
J A
JA
Department of Ecology & Evolution, 650 Life Sciences Bldg, SUNY-Stony Brook, Stony Brook, NY 11794, USA. jbanta@life.bio.sunysb.edu
Pigliucci
M
M
D000704:Analysis of Variance; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D035264:Flowers / Q000254:growth & development*; D018525:Germination / Q000235:genetics; D005875:Gibberellins / Q000235:genetics*; D018519:Meristem / Q000502:physiology*; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D025341:Principal Component Analysis
D005875:Gibberellins