title: Reevaluation and reduction of a PCR bias caused by reannealing of templates.
abstract: We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template reannealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealing step would cause the bias.
authors.affiliation: Kankyo Engineering Co., Ltd., Kisarazu, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kurata
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kanagawa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Magariyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takatsu
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Yamada
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Yokomaku
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamagata
mesh_terms: D000192:Actinomycetales / Q000235:genetics; D015982:Bias; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D011551:Pseudomonas fluorescens / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D013698:Templates, Genetic
chemical_list: D012336:RNA, Ribosomal, 16S