The application of NIR Raman spectroscopy in the assessment of serum thyroid-stimulating hormone in rats.
Serum blood samples of euthyroid and thyroidectomized rats treated with thyrotropin-releasing hormone (TRH) were analyzed on aluminum substrates using the near-infrared Raman spectroscopy (830 nm). Spectra of thyroid-stimulating hormone (TSH), TRH and prolactin standards were obtained. Differences between Raman spectra profiles of control and Tx + TRH samples groups were found. These differences were confirmed by the linear discriminant analysis (LDA), which presents a good classification between groups. It is supposed that these differences are produced by the increment of TSH in the thyroidectomized rats.
Medina-Gutiérrez
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Centro de Investigaciones en Optica A.C., Unidad Aguascalientes, Prolong., Constitución 607, Fracc. Reserva Loma Bonita, Apartado Postal 507, C.P. 20200 Aguascalientes, Mexico. cmedina@cio.mx
Quintanar
J Luis
JL
Frausto-Reyes
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Sato-Berrú
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R
D000818:Animals; D016002:Discriminant Analysis; D008297:Male; D025341:Principal Component Analysis; D011388:Prolactin / Q000032:analysis / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D019265:Spectroscopy, Near-Infrared; D013059:Spectrum Analysis, Raman; D013965:Thyroidectomy; D013972:Thyrotropin / Q000097:blood* / Q000737:chemistry*
D011388:Prolactin; D013972:Thyrotropin