title: Simple and rapid procedure suitable for quantitative isolation of low and high molecular weight extracellular nucleic acids.
abstract: The procedure based on binding of nucleic acids with glass surface in presence of chaotropic salts was adapted for efficient isolation of 100-10000 b.p. DNA fragments and 50-10,000 b. RNA fragments. The method provide 90% and 85% efficacy of isolation of 100 b.p. DNA and 100 b. RNA fragments respectively. High molecular weight nucleic acids are isolated with 98% efficacy. Isolated nucleic acids are free from contaminations, influencing nucleic acids modifying enzymes and fluorochromes. The method is rapid, simple and cost-effective.
authors.affiliation: Institute of Bioorganic Chemistry of Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Tamkovich
authors.forename: Pavel P
authors.initials: PP
authors.lastname: Laktionov
authors.forename: Elena Yu
authors.initials: EY
authors.lastname: Rykova
authors.forename: Valentin V
authors.initials: VV
authors.lastname: Vlassov
mesh_terms: D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D009696:Nucleic Acids