title: Chiral separation of natural and unnatural amino acid derivatives by micro-HPLC on a Ristocetin A stationary phase.
abstract: This paper deals with the chiral separation of Fmoc- and Z-derivatives of natural and unnatural sulfur containing amino acids by micro-HPLC. The separations were carried out in microbore columns packed with a new material based on Ristocetin A bonded to 3.5 microm silica gel. The columns were run in the normal phase, polar organic mode as well as in the reversed phase mode, whereby best results were obtained with the reversed-phase mode using mixtures of triethylamine acetate (TEAA) buffer and methanol as mobile phases.
authors.affiliation: Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Universitätsplatz 1, A-8010 Graz, Austria.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Piccinini
authors.forename: Martin G
authors.initials: MG
authors.lastname: Schmid
authors.forename: Tamara
authors.initials: T
authors.lastname: Pajpanova
authors.forename: Svetlana
authors.initials: S
authors.lastname: Pancheva
authors.forename: Ekatarina
authors.initials: E
authors.lastname: Grueva
authors.forename: Gerald
authors.initials: G
authors.lastname: Gübitz
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification; D000603:Amino Acids, Sulfur / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry / Q000302:isolation & purification; D007536:Isomerism; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D012310:Ristocetin / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000603:Amino Acids, Sulfur; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D012310:Ristocetin; C016719:ristocetin A