High-performance liquid chromatography of di- and trisubstituted aromatic positional isomers on 1,3-alternate 25,27-dipropoxy-26,28-bis-[3-propyloxy]-calix[4]arene-bonded silica gel stationary phase.
A new 1,3-alternate 25,27-dipropoxy-26,28-bis-[3-propyloxy]-calix[4]arene-bonded silica gel stationary phase (1,3-Alt CalixPr) has been prepared and used for the separation of di- and trisubstituted aromatic positional isomers by HPLC. The effect of organic modifier content, pH and column temperature on retention and selectivity of the benzene derivatives were studied. The retention mechanism was also discussed. The results indicated that the stationary phase behaves like a reversed-phase packing. However inclusion, hydrophobic, hydrogen bonding and pi-pi interactions seem to be involved in separation process.
Sliwka-Kaszyńska
Magdalena
M
Department of Organic Chemistry, Gdansk University of Technology, Narutowicza 11/12, 80-952 Gdańsk, Poland. pestka@chem.pg.gda.pl
Jaszczołt
Katarzyna
K
Witt
Dariusz
D
Rachoń
Janusz
J
D047250:Calixarenes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D013816:Thermodynamics
C498313:1,3-alternate 25,27-dipropoxy-26,28-bis-(3-propyloxy)-calix(4)arene; D047250:Calixarenes; D058428:Silica Gel; D012822:Silicon Dioxide