The DNA methylation events in normal and cloned rabbit embryos.
To study the DNA methylation events in normal and cloned rabbit embryos, we investigated the methylation status of a satellite sequence and the promoter region of a single-copy gene using bisulfite-sequencing technology. During normal rabbit embryo development, both sequences maintained hypermethylation status until the 8- to 16-cell stage when progressive demethylation took place. In cloned embryos, the single-copy gene promoter sequence was rapidly demethylated and precociously de novo methylated, while the satellite sequence maintained the donor-type methylation status in all examined stages. Our results indicate that unique sequences as well as satellite sequences may have aberrant methylation patterns in cloned embryos.
Chen
Tao
T
State Key Laboratory of Reproductive Biology, Institute of Zoology, Graduate School, The Chinese Academy of Sciences, Beijing 100080, China.
Zhang
Yan-Ling
YL
Jiang
Yan
Y
Liu
Shu-Zhen
SZ
Schatten
Heide
H
Chen
Da-Yuan
DY
Sun
Qing-Yuan
QY
D000818:Animals; D001483:Base Sequence; D019976:Cloning, Organism / Q000009:adverse effects; D019175:DNA Methylation; D004276:DNA, Satellite / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics / Q000378:metabolism; D011817:Rabbits / Q000196:embryology* / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D013447:Sulfites / Q000378:metabolism
D004276:DNA, Satellite; D037662:Pulmonary Surfactant-Associated Protein A; D013447:Sulfites; C042345:hydrogen sulfite