Serum concentration of the growth medium markedly affects monocyte-derived dendritic cells' phenotype, cytokine production profile and capacities to stimulate in MLR.
We have investigated how the maturation of monocyte-derived dendritic cells (Mo-DC) is affected by the serum concentration of the culture medium. Day 6 DC cultured in 1% human serum were a heterogeneous population of CD1a(-) and CD1a(+) DC that were separated by flow sorting. In contrast, Mo-DC generated in 10% human serum formed a homogenous population of CD1a(-) cells. Other phenotypically immature characteristics also varied, and three subsets were still distinguishable upon maturation in LPS. Furthermore, CD1a(-) DC and CD1a(+) DC from 1% culture conditions were excellent stimulators in MLR, while DC cultured in 10% serum were poor stimulators. Similarly, different cytokine profiles of the three subsets were identified. DC cultured in 1% serum had low expression of interleukin-12 (IL-12) p40 and IL-10 mRNA at day 6. Upon maturation, expression of IL-12 p40 mRNA was upregulated in CD1a(+) DC, whereas the level remained relatively low in CD1a(-) DC. In contrast, DC cultured in 10% had high levels of IL-10 mRNA at day 6 that was downregulated upon maturation. We conclude that the differentiation of monocytes into DC is significantly influenced by the serum concentration of the growth medium with effects on phenotype, cytokine profile and stimulatory activity.
Jakobsen
M A
MA
Department of Clinical Immunology, Odense University Hospital, Odense C, Denmark. marianne.jakobsen@ouh.fyns-amt.dk
Møller
B K
BK
Lillevang
S T
ST
D003470:Culture Media; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D044967:Serum / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology
D003470:Culture Media; D016207:Cytokines; D012333:RNA, Messenger