title: Short report: evaluation of a simple and inexpensive photometric device for the measurement of hemoglobin.
abstract: We have evaluated the accuracy of a simple and inexpensive photometric device (DHT) for the estimation of the blood concentration of hemoglobin by comparison with an automated, high-resolution, flow cytometry-based hematology analyzer (CellDyn 3000) and a centrifugal quantitative buffy coat hematology system (QBC I). We have analyzed the hemoglobin values of 163 individual blood samples. Bland-Altman analysis showed that the methods agreed only poorly: mean differences were 1.0 g/dL with limits of agreement (LOA) of -1.2 g/dL to 3.2 g/dL for the comparison of DHT and CellDyn measurements, 0.5 g/dL with LOA of -2.0 g/dL to 3.0 g/dL for the comparison of DHT with QBC measurements, and 0.5 g/dL with LOA of -1.1 g/dL to 2.1 g/dL for the comparison of QBC with CellDyn measurements. We conclude that the poor agreement of the DHT with the CellDyn does not permit the use of the DHT for critical hemoglobin measurements, particularly in transfusion services.
authors.affiliation: Medical Research Unit, Albert Schweitzer Hospital, BP 118, Lambaréné, Gabon. steffen.borman@uni-tuebingen.de
authors.forename: Steffen
authors.initials: S
authors.lastname: Borrmann
authors.forename: Sunny
authors.initials: S
authors.lastname: Oyakhirome
authors.forename: Gilbert
authors.initials: G
authors.lastname: Esser
authors.forename: Cordula
authors.initials: C
authors.lastname: Trinkle
authors.forename: Saadou
authors.initials: S
authors.lastname: Issifou
authors.forename: Martin P
authors.initials: MP
authors.lastname: Grobusch
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Krishna
authors.forename: Peter G
authors.initials: PG
authors.lastname: Kremsner
mesh_terms: D003124:Colorimetry / Q000295:instrumentation; D005434:Flow Cytometry; D006452:Hemoglobinometry / Q000295:instrumentation*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D019095:Point-of-Care Systems; D011237:Predictive Value of Tests
chemical_list: D006454:Hemoglobins