title: Transcriptional response of avian cells to infection with Newcastle disease virus.
abstract: Newcastle disease virus (NDV) causes widespread disease in poultry and wild-birds throughout the world. cDNA microarray analysis was used to examine the effect of NDV infection on host cell transcription. The results show that NDV infection causes an apparent suppression of the interferon response genes during the early stages of infection. In addition, the results reveal transcriptional silencing of cytoskeletal proteins such as the alpha, beta, and gamma types of actin, and a downregulation of the thioredoxin gene, a likely mediator of apoptosis with possible implications in NDV pathogenesis. Comparative analyses show that a majority of genes that were transcriptionally regulated during infection with another common respiratory pathogen of poultry, the avian pneumovirus, remained unaltered during NDV infection, suggesting that even phylogenetically related viruses elicit unique or "signature" patterns of host transcriptional profiles during infection of host cells.
authors.affiliation: Department of Microbiology, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: Shirin
authors.initials: S
authors.lastname: Munir
authors.forename: Jagdev M
authors.initials: JM
authors.lastname: Sharma
authors.forename: Vivek
authors.initials: V
authors.lastname: Kapur
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D020869:Gene Expression Profiling; D007370:Interferon Type I / Q000235:genetics; D029121:Metapneumovirus / Q000472:pathogenicity; D009522:Newcastle disease virus / Q000472:pathogenicity*; D020411:Oligonucleotide Array Sequence Analysis; D013879:Thioredoxins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D007370:Interferon Type I; D013879:Thioredoxins