title: Identification of peptidyl mimics of bioactive gibberellin recognized by an antibody.
abstract: We screened a phage display peptide library for peptidyl mimotopes of gibberellin against anti-bioactive gibberellin antibody. The peptides obtained were grouped into two homologous sequences and their binding to the antibody was put in competition with free GA(4) but not with GA(4) methylester, suggesting that the peptides behave as mimics of GA(4). As an application, the phage display peptide was shown to work as a tracer for enzyme-linked immunosorbent assay (ELISA) analysis of GA(4).
authors.affiliation: Department of Applied Biological Chemistry, University of Tokyo, Japan.
authors.forename: Haruko
authors.initials: H
authors.lastname: Sekimoto
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Isomaro
authors.initials: I
authors.lastname: Yamaguchi
mesh_terms: D000595:Amino Acid Sequence; D019138:Antibodies, Blocking / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005875:Gibberellins / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D019138:Antibodies, Blocking; D005875:Gibberellins; D019151:Peptide Library; D010455:Peptides; D011956:Receptors, Cell Surface