Preparation of DNA chips using polyamine-oligonucleotide conjugates.
A convenient and efficient method for three-dimensional immobilizing oligonucleotides on glass was developed using oligonucleotide derivatives bearing a polyamine linker (PA-oligo conjugates). Polyamine (polylysine, poly(lysine, phenylalanine), polyethyleneimine) residues stipulate durable fixation of such conjugates to the glass surface with a high yield (90-95%). A DNA fragment (414-mer) is hybridized specifically to an immobilized oligonucleotide.
Levina
Asya
A
Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. Levina@niboch.nsc.ru
Mikhaleva
Elena
E
Zarytova
Valentina
V
D001483:Base Sequence; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*
D009841:Oligonucleotides; D011073:Polyamines