[Study on serum proteome of rat endotoxemia treated by figwort root].
OBJECTIVE
To study the serum proteome of rat endotoxemia treated by figwort root (FR).


METHODS
The differences of serum proteome among rats treated with lipopolysaccharide (LPS), FR, LPS + FR and saline respectively were analyzed by two-dimensional electrophoresis (2DE) assay.


RESULTS
The volumes of sixteen serum proteins (xPr) in LPS induced-endotoxemia group were greatly changed compared with those of the control group. Among them, the volumes of xPr 16, 19 were significantly decreased, and the volumes of xPr 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 18, 23 were significantly increased. When treated with FR, the volumes of xPr 1, 6, 7, 8, 9, 11, 12, 14, 18, 23 were significantly decreased, and the volumes of xPr 8, 9, 11, 12, 23, 14 were back to normal level. Two factors statistic analysis showed that FR had interaction with LPS for xPr 1, 5, 8, 10, 11, 12, 18, 19, 20, 21, 22, and FR might be the functional antagonist of LPS. We also observed that the volumes of xPr 10, 13, 15, 20, 21, 22 were found to change significantly only in FR treated group but not in LPS treated group or control group. Interestingly, the volume of xPr 13, 20, 21, 22 were increased and the volume of xPr 10, 15 were decreased.


CONCLUSIONS
The molecular basis of therapeutic effect of FR on endotoxemia might be through the regulation of xPr 1, 6, 7, 8, 9, 11, 12, 14, 18, 23. We can use proteomic techniques to study the molecular mechanisms of diseases treated by functional Chinese herbs and the combination of different herbs is necessary for the treatment of endotoxemia, as FR can not regulated all the changed proteins induced by LPS.
Xie
Wen-guang
WG
Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China. xiewenguang@hotmail.com
Shao
Ning-sheng
NS
Ma
Xiao-chang
XC
Ding
Qin-xue
QX
Zhao
Xin
X
Liu
Nong-le
NL
Wei
Yu-shu
YS
Wang
Hui-xin
HX
Chen
Ke-ji
KJ
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D019446:Endotoxemia / Q000097:blood* / Q000139:chemically induced; D007275:Injections, Intravenous; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D032266:Scrophularia / Q000737:chemistry
D001798:Blood Proteins; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D020543:Proteome