title: DNA biosensors based on self-assembled carbon nanotubes.
abstract: DNA biosensors based on self-assembled multi-walled carbon nanotubes (MWNTs) were described in this paper, in which the probe DNA oligonucleotides were immobilized by forming covalent amide bonds between carboxyl groups at the nanotubes and amino groups at the ends of the DNA oligonucleotides. Hybridization between the probe and target DNA oligonucleotides was confirmed by the changes in the voltammetric peak of the indicator of methylene blue. Our results demonstrate that the DNA biosensors based on self-assembled MWNTs had a higher hybridization efficiency compared to those based on random MWNTs. In addition, the developed DNA biosensors also had a high selectivity of hybridization detection.
authors.affiliation: Department of Physics, School of Electronics and Physical Sciences, University of Surrey, Guildford GU2 7XH, UK. s.wang@surrey.ac.uk
authors.forename: S G
authors.initials: SG
authors.lastname: Wang
authors.forename: Ruili
authors.initials: R
authors.lastname: Wang
authors.forename: P J
authors.initials: PJ
authors.lastname: Sellin
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017403:In Situ Hybridization / Q000295:instrumentation* / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure*; D015345:Oligonucleotide Probes / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D037742:Nanotubes, Carbon; D015345:Oligonucleotide Probes; D004247:DNA