The glycosylation of human serum IgD and IgE and the accessibility of identified oligomannose structures for interaction with mannan-binding lectin.
Analysis of the glycosylation of human serum IgD and IgE indicated that oligomannose structures are present on both Igs. The relative proportion of the oligomannose glycans is consistent with the occupation of one N-linked site on each heavy chain. We evaluated the accessibility of the oligomannose glycans on serum IgD and IgE to mannan-binding lectin (MBL). MBL is a member of the collectin family of proteins, which binds to oligomannose sugars. It has already been established that MBL binds to other members of the Ig family, such as agalactosylated glycoforms of IgG and polymeric IgA. Despite the presence of potential ligands, MBL does not bind to immobilized IgD and IgE. Molecular modeling of glycosylated human IgD Fc suggests that the oligomannose glycans located at Asn(354) are inaccessible because the complex glycans at Asn(445) block access to the site. On IgE, the additional C(H)2 hinge domain blocks access to the oligomannose glycans at Asn(394) on one H chain by adopting an asymmetrically bent conformation. IgE contains 8.3% Man(5)GlcNAc(2) glycans, which are the trimmed products of the Glc(3)Man(9)GlcNAc(2) oligomannose precursor. The presence of these structures suggests that the C(H)2 domain flips between two bent quaternary conformations so that the oligomannose glycans on each chain become accessible for limited trimming to Man(5)GlcNAc(2) during glycan biosynthesis. This is the first study of the glycosylation of human serum IgD and IgE from nonmyeloma proteins.
Arnold
James N
JN
Medical Research Council (MRC) Immunochemistry Unit and Oxford Glycobiology Institute, University of Oxford, United Kingdom. james.arnold@bioch.ox.ac.uk
Radcliffe
Catherine M
CM
Wormald
Mark R
MR
Royle
Louise
L
Harvey
David J
DJ
Crispin
Max
M
Dwek
Raymond A
RA
Sim
Robert B
RB
Rudd
Pauline M
PM
D002236:Carbohydrate Conformation; D004797:Enzyme-Linked Immunosorbent Assay; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D006834:Hydrazines / Q000378:metabolism; D007072:Immunoglobulin D / Q000097:blood* / Q000737:chemistry; D007073:Immunoglobulin E / Q000097:blood* / Q000737:chemistry; D008358:Mannose / Q000378:metabolism*; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011489:Protein Denaturation; D017510:Protein Folding
D006834:Hydrazines; D007072:Immunoglobulin D; D037601:Mannose-Binding Lectin; D009844:Oligosaccharides; D011134:Polysaccharides; C028569:oligomannoside; C029424:hydrazine; D007073:Immunoglobulin E; D006026:Glycoside Hydrolases; D008358:Mannose