title: Simultaneous immunoaffinity column cleanup and HPLC analysis of aflatoxins and ochratoxin A in Spanish bee pollen.
abstract: Bee pollen is a major substrate for mycotoxins growth when no prompt and adequate drying is performed by the beekeeper after collection by bees. Regulatory limits for aflatoxins and ochratoxin A are currently in force in the European Union for a rising list of foodstuffs, but not for this. An immunoaffinity column cleanup process has been applied prior to the analysis of aflatoxins B(1), B(2), G(1), and G(2) and ochratoxin A (OTA). Optimization of the HPLC conditions has involved both a gradient elution and a wavelength program for the separation and fluorimetric quantitation of all five mycotoxins at their maximum excitation and emission values of wavelength in a single run. The higher limit of detection (mug/kg) was 0.49 for OTA and 0.20 for aflatoxin B(1). Repeatability (RSDr) at the lower limit tested ranged from 9.85% for OTA to 6.23% for aflatoxin G(2), and recoveries also at the lower spiked level were 73% for OTA and 81% for aflatoxin B(1). None of the 20 samples assayed showed quantifiable values for the five mycotoxins.
authors.affiliation: Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. rgvill@usal.es
authors.forename: Rafael J
authors.initials: RJ
authors.lastname: Garcia-Villanova
authors.forename: Carlos
authors.initials: C
authors.lastname: Cordón
authors.forename: Ana M
authors.initials: AM
authors.lastname: González Paramás
authors.forename: P
authors.initials: P
authors.lastname: Aparicio
authors.forename: M Eugenia
authors.initials: ME
authors.lastname: Garcia Rosales
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D001516:Bees; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D011058:Pollen / Q000737:chemistry*; D013030:Spain
chemical_list: D000348:Aflatoxins; D009793:Ochratoxins; C025589:ochratoxin A