Elaboration of a crude antigen ELISA for serodiagnosis of caprine neosporosis: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri Lanka.
The protozoan parasite N. caninum is a major pathogen in cattle and dogs. However, clinical symptoms are occasionally described for other potential hosts. Natural abortion in goats due to N. caninum has been rarely reported and only little data is available on the seroprevalence of N. caninum in this species. In the present study, 486 goats from Sri Lanka were tested in a crude antigen ELISA for the presence of serum antibodies against N. caninum. Additionally, the sera were analysed by N. caninum-Western blot and indirect fluorescence antibody test (IFAT). In all three tests applied, only three sera (0.7%) were scored clearly positive for anti-N. caninum antibodies. The optimal correlation between ELISA, IFAT, and Western blot confirms the suitability of the ELISA for large-scale seroepidemiologic studies, not only in cattle but also in goats.
Naguleswaran
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A
Institute of Parasitology, University of Bern, Laenggass-Str. 122, CH-3001 Bern, Switzerland.
Hemphill
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Rajapakse
R P V J
RP
Sager
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H
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D015153:Blotting, Western / Q000662:veterinary; D003048:Coccidiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D015511:Goat Diseases / Q000175:diagnosis / Q000453:epidemiology*; D006041:Goats; D018688:Neospora / Q000276:immunology*; D016036:Seroepidemiologic Studies; D013188:Sri Lanka / Q000453:epidemiology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan