Physicochemical characteristics and biological activities of seasonal atmospheric particulate matter sampling in two locations of Paris.
Fine particulate matter present in urban areas seems to be incriminated in respiratory disorders. The aim of this study was to relate physicochemical characteristics of PM2.5 (particulate matter collected with a 50% efficiency for particles with an aerodynamic diameter of 2.5 microm) to their biological activities toward a bronchial epithelial cell line 16-HBE. Two seasonal sampling campaigns of particles were realized, respectively, in a kerbside and an urban background station in Paris. Sampled-PM2.5 mainly consist of particles with a size below 1 microm and are mainly composed of soot as assessed by analytical scanning electron microscopy. The different PM2.5 samples contrasted in their PAH content, which was the highest in the kerbside station in winter, as well as in their metal content. Kerbside station samples were characterized by the highest Fe and Cu content, which appears correlated to their hydroxyl radical generating properties measured by electron paramagnetic resonance. Particles were compared by their capacity to induce cytotoxicity, intracellular ROS production, and proinflammatory cytokine release (GM-CSF and TNF-alpha). At a concentration of 10 microg/cm2, all samples induced peroxide production and cytokine release to the similar extent in the absence of cytotoxicity. In conclusion, whereas the PM2.5 samples differ by their PAH and metal composition, they induce the same biological responses likely either due to components bioavailability and/ or interactions between PM components.
Baulig
Augustin
A
Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Université Paris 7, Case Courrier 7073, 75251 Paris Cédex 05, France. baulig@paris7.jussieu.fr
Poirault
Jean-Jacques
JJ
Ausset
Patrick
P
Schins
Roel
R
Shi
Tingming
T
Baralle
Delphine
D
Dorlhene
Pascal
P
Meyer
Martine
M
Lefevre
Roger
R
Baeza-Squiban
Armelle
A
Marano
Francelyne
F
D000393:Air Pollutants / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D001980:Bronchi / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010297:Paris; D010316:Particle Size; D010545:Peroxides / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012140:Respiratory Tract Diseases / Q000139:chemically induced*; D012621:Seasons; D014504:Urban Health; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity*
D000393:Air Pollutants; D016207:Cytokines; D010545:Peroxides; D017382:Reactive Oxygen Species; D001335:Vehicle Emissions