Detection of prothrombin and osteopontin in a renal stone found in a hyperuricemic patient using 2D-PAGE and LC-MS analysis.
The liquid chromatography-mass spectrometry (LC-MS) following on from the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was applied for the analysis of proteins in a renal stone found in a hyperuricemic patient. This technique was sensitive enough to detect small quantities of proteins even in a renal stone.
Kaneko
K
K
Central Laboratory of Analytical Biochemistry, Faculty of Medicine, Teikyo University, Tokyo, Japan.
Yamanobe
T
T
Nakagomi
K
K
Mawatari
K
K
Onoda
M
M
Fujimori
S
S
D000042:Absorption; D000595:Amino Acid Sequence; D002853:Chromatography, Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D033461:Hyperuricemia / Q000473:pathology*; D007669:Kidney Calculi / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D008969:Molecular Sequence Data; D053495:Osteopontin; D011516:Prothrombin / Q000032:analysis / Q000096:biosynthesis*; D012795:Sialoglycoproteins / Q000032:analysis / Q000096:biosynthesis*; D021241:Spectrometry, Mass, Electrospray Ionization
C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D011516:Prothrombin