Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs.
Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.
Ambrósio
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Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Unesp, Rod. Araraquara-Jaú km 1, 14801-902 Araraquara, SP, Brazil.
Barbosa
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CF
Vianna
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VF
Cicarelli
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RM
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D005091:Exons / Q000235:genetics; D007438:Introns / Q000235:genetics; D009583:Nitrofurazone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000187:drug effects*; D016053:RNA, Protozoan / Q000187:drug effects*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D012333:RNA, Messenger; D016053:RNA, Protozoan; D014344:Trypanocidal Agents; C483719:hydroxymethylnitrofurazone; D009583:Nitrofurazone