title: Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries.
abstract: Degenerate oligonucleotide primers, designed based on conserved regions of Nucleotide Binding Site (NBS) domains from previously cloned plant resistance genes, were used to isolate Resistance Gene Analogues (RGAs) from wild and cultivated strawberries. Seven distinct families of RGAs of the NBS-LRR type were identified from two related wild species, Fragaria vesca and F. chiloensis, and six different Fragaria x ananassa cultivars. With one exception (GAV-3), the deduced amino acid sequences of strawberry RGAs showed strong similarity to TIR (Toll Interleukin I Receptor)-type R genes from Arabidopsis, tobacco and flax, suggesting the existence of common ancestors. GAV-3 seemed to be more closely related to the non-TIR type. Further studies showed that the recombination level and the ratio of non-synonymous to synonymous substitutions within families were low. These data suggest that NBS-encoding sequences of RGAs in strawberry are subject to a gradual accumulation of mutations leading to purifying selection, rather than to a diversifying process. The present paper is the first report on RGAs in strawberry.
authors.affiliation: Dpto. de Bioquímica de la Nutrición, Instituto de Química Biológica "Dr. Bernabé Bloj" (UNT), INSIBIO (CONICET-UNT), Chacabuco 461, 4000 Tucumán, Argentina.
authors.forename: M G
authors.initials: MG
authors.lastname: Martínez Zamora
authors.forename: A P
authors.initials: AP
authors.lastname: Castagnaro
authors.forename: J C
authors.initials: JC
authors.lastname: Díaz Ricci
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003433:Crosses, Genetic; D017931:DNA Primers; D031985:Fragaria / Q000235:genetics* / Q000276:immunology; D017343:Genes, Plant; D014644:Genetic Variation; D007113:Immunity, Innate / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers