Use of species-directed 16S rRNA gene PCR primers for detection of Atopobium vaginae in patients with bacterial vaginosis.
Recent studies suggest that the association between a metronidazole-resistant anaerobe, Atopobium vaginae, and bacterial vaginosis (BV) warrants further investigation. In the present study, specific primers enhanced detection of A. vaginae and provided additional evidence that this bacterium is prevalent among patients with BV but absent among patients with normal vaginal flora.
Ferris
Michael J
MJ
Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA, USA. mferris@chnola-research.org
Masztal
Alicia
A
Martin
David H
DH
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D020459:Genes, rRNA; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014621:Vagina / Q000382:microbiology; D016585:Vaginosis, Bacterial / Q000175:diagnosis / Q000382:microbiology*
D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S