title: Resistance artery vasodilation to magnesium sulfate during pregnancy and the postpartum state.
abstract: This study compared the vasodilatory responses to magnesium sulfate (MgSO(4)) of cerebral and mesenteric resistance arteries and determined whether the responses varied between different gestational groups. Third-order branches (<200 microm) of the posterior cerebral (PCA) and mesenteric arteries (MA) were dissected from nonpregnant (NP; n = 6), late pregnant (LP; day 19, n = 6), and postpartum (PP; day 3, n = 6) Sprague-Dawley rats. A concentration-response curve was performed by replacing the low-MgSO(4) (1.2 mM) HEPES buffer solution with increasing concentrations of MgSO(4) (4, 6, 8, 16, and 32 mM) and measuring lumen diameter at each concentration. All groups exhibited concentration-dependent dilation to MgSO(4), decreasing the amount of tone in the vessels. However, MA were significantly more sensitive to MgSO(4) than PCA. Whereas there was no difference in the response between different gestational groups in MA, the PCA from the LP and PP groups showed a significantly diminished response to MgSO(4). The percent dilation at 32 mM MgSO(4) for PCA versus MA in NP, LP, and PP animals was 36 +/- 2 vs. 51 +/- 7% (P < 0.05), 19 +/- 9 vs. 54 +/- 6% (P < 0.01 vs. PCA and NP), and 12 +/- 5 vs. 52 +/- 11% (P < 0.01 vs. PCA and NP). These results demonstrate that MgSO(4) is a vasodilator of small resistance arteries in the cerebral and mesenteric vascular beds. The refractory responses of the PCA in LP and PP groups demonstrate changes in the cerebrovascular vasodilatory mechanisms with gestation. The greater sensitivity of the MA to MgSO(4)-induced vasodilation suggests that the prophylactic effect of MgSO(4) on eclamptic seizures may be more closely related to the lowering of systemic blood pressure than to an effect on cerebral blood flow.
authors.affiliation: Dept. of Neurology, University of Vermont, 89 Beaumont Ave., Given Bldg., Rm. C454, Burlington, VT 05405, USA. Marilyn.Cipolla@uvm.edu
authors.forename: Anna G
authors.initials: AG
authors.lastname: Euser
authors.forename: Marilyn J
authors.initials: MJ
authors.lastname: Cipolla
mesh_terms: D000818:Animals; D002536:Cerebral Arteries / Q000187:drug effects / Q000502:physiology; D005260:Female; D008278:Magnesium Sulfate / Q000494:pharmacology*; D008638:Mesenteric Arteries / Q000187:drug effects / Q000502:physiology; D049590:Postpartum Period; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015149:Tocolytic Agents / Q000494:pharmacology*; D014655:Vascular Resistance; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D015149:Tocolytic Agents; D014665:Vasodilator Agents; D008278:Magnesium Sulfate