[Overproduction of agouti-protein did not affect cAMP level but increased steroidogenesis in adrenal glands under forskolin stimulation].
Mutation Agouti yellow (Ay) in mice Ay/a results in overproduction of agouti protein (AP), adult onset of obesity, increased corticosterone responses to restrain stress as compared with a/a mice (absence of AP). The enhanced corticosterone response in restrained Ay/a-mice compared with restrained a/a-mice occurred in result of increased adrenal reactivity to ACTH. The purpose of this work was to investigate the influence of AP overproduction on adenylate cyclase (AC) activity and steroidogenesis in forskolin stimulated adrenal cells. To estimate obesity influence, these parameters were measured in young (3 weeks) and adult (15 weeks) animals. The data obtained demonstrated that AP overproduction and the obesity did not affect the AC activity. However, forskolin stimulated corticosterone production in Ay/a-mice was higher than in a/a-mice (in young--during 0.5 h, in adult--during 3 hrs of incubation). So AP overproduction and obesity affect the corticosterone production. We hypothesize that AP overproduction affects steroidogenesis gene expression: accelerates gene activation in ontogenesis and increases enzyme de novo synthesis during long-term stimulation in adults.
Iakovlev
T V
TV
Karkaeva
N R
NR
Shevchenko
A Iu
AIu
Makarova
E N
EN
Bazhan
N M
NM
D000262:Adenylyl Cyclases / Q000378:metabolism; D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D054366:Agouti Signaling Protein; D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D003345:Corticosterone / Q000032:analysis / Q000096:biosynthesis; D000242:Cyclic AMP / Q000032:analysis / Q000096:biosynthesis; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation / Q000235:genetics; D013256:Steroids / Q000032:analysis / Q000096:biosynthesis*; D015533:Transcriptional Activation
D054366:Agouti Signaling Protein; D036341:Intercellular Signaling Peptides and Proteins; D013256:Steroids; C515183:a protein, mouse; D005576:Colforsin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D003345:Corticosterone