[The effects of tissue factor/activated factor VII complex on the invasion and metastasis of human ovarian cancer].
OBJECTIVE
To explore the role of tissue factor/activated factor VII (TF/FVIIa) complex in human ovarian cancer invasion and metastasis.


METHODS
(1) Constructed an expression vector of TF, pcDNA3-TF and established a human ovarian cell line A2780/TF expressing high level TF by using molecular cloning and gene transfection techniques. (2) By Boyden chamber assay to count the numbers of A2780 and A2780/TF cells that penetrated the matrigel to the back of PVPF membrane after FVIIa stimulation. (3) BALB/c nude mice were used to establish experimental model of metastasis with A2780 or A2780/TF and the lung tissue sections were examined by microscopy for cancer metastasis.


RESULTS
(1) Compared with their parental A2780 cells, A2780/TF cells expressed high level of TF mRNA (3.99 +/- 0.15 vs 0.97 +/- 0.23, P < 0.01) and TF antigen on cell surface \[(48.56 +/- 9.53)% vs (2.73 +/- 1.15)%, P < 0.01\]. (2) After stimulation, the A2780/TF cell number on the back of PVPF membrane increased from basal level 157.3 +/- 19.2 to 447.7 +/- 39.4 (P < 0.01), which could decreased to basal level when coincubated with anti-TF antibody. (3) Cancer metastasis was found in 22.2% of nude mice transplanted with A2780 cells, while in 88.9% of those transplanted with A2780/TF cells.


CONCLUSIONS
TF could promote the invasion and metastasis of human ovarian cancer cells through TF/FVIIa pathway.
Fang
Jun
J
Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Wei
Wen-ning
WN
Xia
Ling-hui
LH
Song
Shan-jun
SJ
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D003001:Cloning, Molecular; D015942:Factor VIIa / Q000235:genetics* / Q000502:physiology; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D013925:Thromboplastin / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014183:Transplantation, Heterologous
D013925:Thromboplastin; D015942:Factor VIIa