title: Microarray analysis of temperature-induced transcriptome of Yersinia pestis.
abstract: Yersinia pestis, the etiologic agent of plague, must acclimatize itself to temperature shifts between the temperature (26 C) for flea blockage and the body temperature (37 C) of warm-blooded hosts during its life cycle. Here a whole-genome DNA microarray was used to investigate transcriptional regulation upon the upshift of growth temperature from 26 to 37 C in a chemically defined medium. Four hundred and one genes were regulated differentially under the two temperatures. About 39% of these genes were up-regulated at 37 C, whereas 61% were down-regulated. Temperature-induced changes occurred at the level of transcription of genes encoding proven or predicted virulence factors, regulators, metabolism-associated proteins, prophages, and hypothetical proteins. Strikingly, many gene clusters displayed a co-transcription pattern in response to temperature upshift. Our data provided a genome-wide profile of gene transcription induced by temperature shift and should shed light on the pathogenicity and host-microbe interaction of this deadly pathogen.
authors.affiliation: Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Pang
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Jingyue
authors.initials: J
authors.lastname: Bao
authors.forename: Zongzhong
authors.initials: Z
authors.lastname: Tong
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhai
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Peitang
authors.initials: P
authors.lastname: Huang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020543:Proteome; D005423:Siphonaptera; D013696:Temperature; D014158:Transcription, Genetic; D015010:Yersinia pestis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome