title: Decreased cellular permeability to H2O2 protects Saccharomyces cerevisiae cells in stationary phase against oxidative stress.
abstract: The higher resistance of stationary-phase Saccharomyces cerevisiae to H2O2 when compared with exponential phase is well characterized, but the molecular mechanisms underlying it remain mostly unknown. By applying the steady-state H2O2-delivery model, we show that (a) cellular permeability to H2O2 is five times lower in stationary--than in exponential phase; (b) cell survival to H2O2 correlates with H2O2 cellular gradients for a variety of cells; and, (c) cells in stationary phase are predicted to be more susceptible to intracellular H2O2 than in exponential phase. In conclusion, limiting H2O2 diffusion into cells is a key protective mechanism against extracellular H2O2.
authors.affiliation: Grupo de Bioquímica dos Oxidantes e Antioxidantes, Centro de Química e Bioquímica, P-1749-016 Lisboa, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Sousa-Lopes
authors.forename: F
authors.initials: F
authors.lastname: Antunes
authors.forename: L
authors.initials: L
authors.lastname: Cyrne
authors.forename: H S
authors.initials: HS
authors.lastname: Marinho
mesh_terms: D002453:Cell Cycle / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology*; D002470:Cell Survival; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D016877:Oxidants; D006861:Hydrogen Peroxide