title: Simultaneous injection of bone marrow cells and stromal cells into bone marrow accelerates hematopoiesis in vivo.
abstract: We have previously demonstrated that stromal cells can support the proliferation and differentiation of hematopoietic cells in vitro and in vivo and that a major histocompatibility complex restriction exists between hematopoietic stem cells and stromal cells. We have also found that intra-bone marrow (IBM) injection of allogeneic bone marrow cells (BMCs) leads to more rapid reconstitution of hematopoietic cells than intravenous injection. In the present study, we examine the effect of simultaneous injection of stromal cells and BMCs into the same bone marrow on the recovery of donor hematopoietic cells and demonstrate that simultaneous IBM injection of BMCs plus stromal cells is more effective in reconstituting recipients with donor hematopoietic cells than intravenous injection of BMCs plus stromal cells or IBM injection of BMCs alone.
authors.affiliation: First Department of Pathology, Kansai Medical University, Moriguchi, Japan.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Keizo
authors.initials: K
authors.lastname: Minamino
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Iwasaki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hisha
authors.forename: Chang Ye
authors.initials: CY
authors.lastname: Song
authors.forename: Kimihide
authors.initials: K
authors.lastname: Kusafuka
authors.forename: Keiji
authors.initials: K
authors.lastname: Nakano
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Koike
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Eitaku
authors.initials: E
authors.lastname: Koh
authors.forename: Yunze
authors.initials: Y
authors.lastname: Cui
authors.forename: Chunfu
authors.initials: C
authors.lastname: Li
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D004730:Endothelium, Vascular / Q000166:cytology*; D005434:Flow Cytometry; D015870:Gene Expression; D006410:Hematopoiesis; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology*; D018919:Neovascularization, Physiologic; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger