Reevaluation and reduction of a PCR bias caused by reannealing of templates.
We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template reannealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealing step would cause the bias.
Kurata
Shinya
S
Kankyo Engineering Co., Ltd., Kisarazu, Japan.
Kanagawa
Takahiro
T
Magariyama
Yukio
Y
Takatsu
Kyoko
K
Yamada
Kazutaka
K
Yokomaku
Toyokazu
T
Kamagata
Yoichi
Y
D000192:Actinomycetales / Q000235:genetics; D015982:Bias; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D011551:Pseudomonas fluorescens / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D013698:Templates, Genetic
D012336:RNA, Ribosomal, 16S