title: Association of human leukocyte antigen DQ1 and dengue fever in a white Southern Brazilian population.
abstract: Dengue is an infectious disease of viral etiology transmitted by the mosquitoes Aedes aegypti, A. albopictus, and A. scutellaris. It can develop either as a benign form or as a severe hemorrhagic form. Previous work showed an association of the hemorrhagic form with human leukocyte antigens (HLA), suggesting a role of genetic factors in disease susceptibility. Nevertheless, data on HLA association with the classical form of the disease is scarce in literature. Sixty-four patients and 667 normal individuals, living in the state of Parana, Southern Brazil, were used as test and control group, respectively. The patients developed the disease during a virus 1 dengue outbreak either in Maringa city in 1995 (47) or in Paranavai city in 1999 (17). The diagnostic was confirmed through serology and/or viral culture. HLA class I and II typing was performed by the classical microlynfocitotoxicity test using monoclonal antisera and fluorobeads. Qui-square statistical analysis confirmed a positive association with HLA-DQ1 (76.6% vs 57.7%; p = 0.005243; pc = 0.026215). HLA-DR1 also presented an increased frequency in the test group, not statistically significant after p correction though (32.8% vs 15.9%; p = 0.005729; pc = 0.080206). In conclusion, genetic factors may play a role on the susceptibility to the classical dengue, virus 1, in the Brazilian population. Further independent studies should be performed in the Brazilian population to confirm these preliminary data.
authors.affiliation: Departamento de Análises Clínicas, Universidade Estadual de Maringá, Av. Colombo 5790, 87020-900 Maringá, PR, Brazil.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Polizel
authors.forename: Danilo
authors.initials: D
authors.lastname: Bueno
authors.forename: Jeane Eliete L
authors.initials: JE
authors.lastname: Visentainer
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Sell
authors.forename: Sueli Donizete
authors.initials: SD
authors.lastname: Borelli
authors.forename: Luiza Tamie
authors.initials: LT
authors.lastname: Tsuneto
authors.forename: Marcia Machado O
authors.initials: MM
authors.lastname: Dalalio
authors.forename: Maria Teresa M
authors.initials: MT
authors.lastname: Coimbra
authors.forename: Ricardo Alberto
authors.initials: RA
authors.lastname: Moliterno
mesh_terms: D001938:Brazil; D016022:Case-Control Studies; D003715:Dengue / Q000235:genetics* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D012307:Risk Factors
chemical_list: D006683:HLA-DQ Antigens