[Cellular compatibility of small intestinal submucosa in vitro].
This study was aimed to evaluate the cellular compatibility of the small intestinal submucosal(e) (SIS). Prepared by use of pig jejunum. SIS were cocultured with human embryonic periosteal osteoblasts (HEPOB), human embryonic skin fibroblasts (HESFB) and rabbit renal vascular endothelial cells (RRVEC) respectively. The cell growth, attachment, cell cycle, cell apoptosis rate were detected to evaluate the cellular compatibility of SIS. The three kinds of cells attached onto SIS and grew well. SIS accelerated the growth of RRVEC. No effects of SIS were detected on cell cycle and cell apoptosis rate in the three kinds of cells. SIS has good cellular compatibility without cytotoxicity. The porous structure of SIS is suited for the growth of HEPOB, HESFB and RRVEC in three dimensions in the scaffold. SIS is a good bio-derived material of tissue engineering.
Luo
Jingcong
J
Institute of Reparative and Reconstructive Surgery, Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang
Zhiming
Z
Li
Xiuqun
X
Deng
Li
L
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D005109:Extracellular Matrix / Q000502:physiology*; D006648:Histocompatibility; D007413:Intestinal Mucosa / Q000166:cytology*; D007583:Jejunum / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D013552:Swine; D023822:Tissue Engineering