title: Ultrastructure of right ventricular myocardium subjected to acute pressure load.
abstract: BACKGROUND
  Ultrastructural data on acute right ventricular pressure load in pigs are rare.
  
  
  METHODS
  In control (n = 7) and banding groups (n = 6), right ventricular pressure (micromanometry) and function (sonomicrometry) were measured. Right ventricular pressure was increased 2.5-fold in the banding group by pulmonary artery constriction. Right ventricular biopsies were taken at baseline and after 6 h and processed for electron microscopy. Parameters of cellular injury were determined stereologically. Three perfusion -fixed hearts were investigated qualitatively in each group.
  
  
  RESULTS
  Stereology revealed an increase in the sarcoplasmic volume fraction and the cellular edema index in the banding group ( p < 0.05). Mitochondrial surface-to-volume ratio and volume fraction did not show significant alterations. Subendocardial edema and small amounts of severely injured myocytes were observed in the perfusion-fixed hearts after banding. Ultrastructure was normal in controls. After an initial increase, the right ventricular work index declined progressively in the banding group but remained unchanged in controls.
  
  
  CONCLUSIONS
  Ultrastructural alterations resulting from acute right ventricular pressure load were characterized by edema of subendocardial myocytes and single cell necrosis. Focal adrenergic overstimulation and mechanical stress are probably more relevant in the pathogenesis of these lesions than ischemia.
authors.affiliation: Department of Anatomy, Division Electron Microscopy, Kreuzbergring 36, 37075 Göttingen, Germany. christian.muehlfeld@medizin.uni-goettingen.de
authors.forename: C
authors.initials: C
authors.lastname: Mühlfeld
authors.forename: M
authors.initials: M
authors.lastname: Coulibaly
authors.forename: H
authors.initials: H
authors.lastname: Dörge
authors.forename: C
authors.initials: C
authors.lastname: Sellin
authors.forename: O
authors.initials: O
authors.lastname: Liakopoulos
authors.forename: C
authors.initials: C
authors.lastname: Ballat
authors.forename: J
authors.initials: J
authors.lastname: Richter
authors.forename: F
authors.initials: F
authors.lastname: Schoendube
mesh_terms: D000208:Acute Disease; D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D006003:Glycogen / Q000378:metabolism; D006321:Heart / Q000503:physiopathology; D006352:Heart Ventricles / Q000648:ultrastructure; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000378:metabolism / Q000648:ultrastructure; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D010477:Perfusion; D011651:Pulmonary Artery / Q000503:physiopathology; D013552:Swine; D014661:Vasoconstriction
chemical_list: D006003:Glycogen