title: Comparison of mRNA expression levels determined with TaqMan and competitive template RT-PCR.
abstract: Two methods for measurement of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) mRNA expression were compared. Although the relative mRNA levels compared to beta-actin measured with competitive template RT-PCR were different from the data obtained with a TaqMan based PCR, a significant correlation between the two assays was found.
authors.affiliation: Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Mauritz
authors.forename: W
authors.initials: W
authors.lastname: Schwabe
authors.forename: P
authors.initials: P
authors.lastname: Haeusler
authors.forename: P
authors.initials: P
authors.lastname: Noordhuis
authors.forename: K
authors.initials: K
authors.lastname: Smid
authors.forename: H L
authors.initials: HL
authors.lastname: McLeod
authors.forename: D
authors.initials: D
authors.lastname: Behnke
authors.forename: C J
authors.initials: CJ
authors.lastname: van Groeningen
authors.forename: G J
authors.initials: GJ
authors.lastname: Peters
mesh_terms: D000199:Actins / Q000378:metabolism; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D015179:Colorectal Neoplasms / Q000378:metabolism; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013940:Thymidylate Synthase / Q000378:metabolism
chemical_list: D000199:Actins; D000964:Antimetabolites, Antineoplastic; D012333:RNA, Messenger; D042943:Dihydrouracil Dehydrogenase (NADP); D013940:Thymidylate Synthase; D005472:Fluorouracil