title: The influence of the non-nucleotide insert on the hybridization properties of oligonucleotides.
abstract: The effect of different non-nucleotide inserts incorporated into oligonucleotide chains on their hybridization properties was studied by the method of thermal denaturation. Various types of alkyldiols and oligoethylene glycols were used as inserts modifying oligonucleotide backbone. Such modification of oligonucleotides caused the destabilization of their complementary complexes. It was shown that the hybridization properties of the modified oligonucleotides depend on several features of inserts: the type, number, length of insertions, and positions of interrupted dinucleotide steps in oligonucleotide chain.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SD RAS, Novosibirsk, Russia. pyshnaya@niboch.ncs.ru
authors.forename: Inna A
authors.initials: IA
authors.lastname: Pyshnaya
authors.forename: Dmitrii V
authors.initials: DV
authors.lastname: Pyshnyi
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Lomzov
authors.forename: Valentina F
authors.initials: VF
authors.lastname: Zarytova
authors.forename: Eugenia M
authors.initials: EM
authors.lastname: Ivanova
mesh_terms: D001483:Base Sequence; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009841:Oligonucleotides