title: Characterization of the promoter of the mouse c-Jun NH2-terminal/stress-activated protein kinase alpha gene.
abstract: We report the isolation of the mouse JNK/SAPKalpha gene, the determination of its exon/intron organization and the characterization of its promoter region. The mouse JNK/SAPKalpha gene spans a region of 36 kbp and contains 13 exons, which represent about 8% of the gene sequence. Major JNK/SAPKalpha splice variants (I and II) are generated by alternative splicing of exons 7 and 8, respectively, whereas minor variants (III and IV) are generated using cryptic sites located inside exon 9. The regulatory elements of the JNK/SAPKalpha gene are located in a 400-bp region placed upstream of the first exon. The gene lacks a TATA element and the initiation of transcription is located inside a 1-kbp CG island. Two regulatory regions located at -98/-69 and -69/-30 were defined by deletion analysis of the promoter.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n. 24007 León, Spain.
authors.forename: Ainhoa I
authors.initials: AI
authors.lastname: Callejo
authors.forename: Emilio
authors.initials: E
authors.lastname: Casanova
authors.forename: Pedro
authors.initials: P
authors.lastname: Calvo
authors.forename: Román
authors.initials: R
authors.lastname: Galetto
authors.forename: José C
authors.initials: JC
authors.lastname: Rodríguez-Rey
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Chinchetru
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D016385:TATA Box; D014158:Transcription, Genetic
chemical_list: D048031:JNK Mitogen-Activated Protein Kinases