title: Glutathione S-transferase in the insect Apis mellifera macedonica kinetic characteristics and effect of stress on the expression of GST isoenzymes in the adult worker bee.
abstract: The glutathione S-transferase present in the adult worker bee Apis mellifera macedonica was purified and analyzed for its physicochemical and kinetic properties. The enzyme is heterodimeric with subunit molecular masses of 29 and 25 kDa, respectively. Two main isoenzymes with distinct kinetic properties are present, with isoelectric points of 7.40 for the alkaline and 4.58 for the acidic forms, respectively. The two enzymes are induced independently by factors such as insecticide treatments and environmental conditions, including low temperatures or starvation.
authors.affiliation: Laboratory of Animal Physiology, Department of Zoology, Faculty of Science, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece. thanpap@bio.auth.gr
authors.forename: Athanasios I
authors.initials: AI
authors.lastname: Papadopoulos
authors.forename: Irene
authors.initials: I
authors.lastname: Polemitou
authors.forename: Pshychoula
authors.initials: P
authors.lastname: Laifi
authors.forename: Astero
authors.initials: A
authors.lastname: Yiangou
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Tananaki
mesh_terms: D000818:Animals; D001516:Bees / Q000201:enzymology*; D003080:Cold Temperature; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000494:pharmacology; D007526:Isoelectric Point; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009570:Nitriles; D010261:Paraoxon / Q000031:analogs & derivatives* / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors; D007306:Insecticides; D007527:Isoenzymes; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D005982:Glutathione Transferase; D010261:Paraoxon; C008480:methylparaoxon