[Effects of siwu tang on protein expression of bone marrow of blood deficiency mice induced by irradiation].
OBJECTIVE
To observe the effects of siwu tang on protein expression of bone marrow of blood deficiency mice and provide the theoretical and experimental basis for understanding the molecular mechanism of blood enriching function of siwu tang.


METHODS
Blood deficiency mice were established by using 3.5 Gy 60Co gamma-ray. With proteomic technologies including two-dimensional electrophoresis, image analysis, in-gel digestion, peptide mass fingerprinting and bioinformatics the proteins of bone marrow of blood deficiency mice were isolated, analyzed, and identified.


RESULTS
Siwu tang could reverse 10 up-regulated and 4 down-regulated proteins of blood deficiency mice bone marrow. Seven of the proteins were likely to be lymphocyte specific protein 1, proteasome 26S ATPase subunit 4, hematopoietic cell protein-tyrosine phosphatase, glyceraldehyde-3-phosphate dehydrogenase, growth factor receptor binding protein 14 and lgals12 respectively.


CONCLUSIONS
Siwu tang can regulate the protein expression of bone marrow of blood deficiency mice and thus promote the growth and differentiation of hematopoietic cells and then exert its effects on blood enriching function.
Guo
Ping
P
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Ma
Zeng-chun
ZC
Li
Ying-fei
YF
Liang
Qian-de
QD
Wang
Ji-feng
JF
Wang
Sheng-qi
SQ
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008516:Medicine, Chinese Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D010750:Phosphoproteins / Q000097:blood; D011506:Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011833:Radiation Injuries, Experimental / Q000097:blood*; D014916:Whole-Body Irradiation
D048868:Adaptor Proteins, Signal Transducing; D004365:Drugs, Chinese Herbal; C099655:GRB14 protein, human; D010750:Phosphoproteins; D011506:Proteins; D020543:Proteome; C093915:lymphocyte-specific protein p50; C403258:si-wu-tang