[Expression of aquaporin-1 and Bcl-2 mRNA in eosinophils of the nasal polyps and its significance].
OBJECTIVE
To study the significance of aquaporin-1 (AQP-1) expression in the eosinophils of nasal polyps. The expression and location of AQP-1 mRNA and apoptosis associated gene Bcl-2 mRNA in nasal polyps were explored.


METHODS
Sixteen nasal polyp samples were collected from 11 women and 5 men aged 20-65 years during routine endonasal surgery. Nasal mucosa specimens from the inferior turbinates of 10 patients with allergic rhinitis (7 women and 3 men, aged 16-58 years), collected during septoplasty, were used as controls. The expression of AQP-1 mRNA and Bcl-2 mRNA was detected in serial adjacent sections by in situ hybridization and eosinophils were examined by stain MGG.


RESULTS
AQP-1 mRNA expression was found in all 16 nasal polyps and in 4 of 10 inferior turbinate tissues, the mean expression rates were (93.16 +/- 13.25)% and (19.54 +/- 4.98)%, respectively. All 16 nasal polyps and 10 control nasal tissues expressed Bcl-2 mRNA, by the average rates of (84.74 +/- 12.10)% and (16.45 +/- 3.12)%, respectively. The expression of AQP-1 mRNA was positively correlated with Bcl-2 mRNA expression in nasal polyps (r = 0.875, P < 0.01).


CONCLUSIONS
AQP-1 contributes to the survival of eosinophils in nasal polyps by keeping the permeation balance of eosinophils.
Guan
Gui-mei
GM
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031, China.
Dong
Zhen
Z
Lü
Mei
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D051398:Aquaporin 1 / Q000378:metabolism*; D016022:Case-Control Studies; D004804:Eosinophils / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
C496020:AQP1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051398:Aquaporin 1