Cloning and characterization of a human cDNA ACAD10 mapped to chromosome 12q24.1.
Mitochondrial fatty acid beta-oxidation is an important energy resource for many mammal tissues. Acyl-CoA dehydrogenases (ACADs) are a family of flavoproteins that are involved in the beta-oxidation of the fatty acyl-CoA derivatives. Deficiency of these ACADs can cause metabolic disorders including muscle fatigue, hypoglycaemia, hepatic lipidosis and so on. By large scale sequencing, we identified a cDNA sequence of 3960 base pairs with a typical acyl-CoA dehydrogenase function domain. RT-PCR result shows that it is widely expressed in human tissues, especially high in liver, kidney, pancreas and spleen. It is hypothesized that this is a novel member of ACADs family.
Ye
Xin
X
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Ji
Chaoneng
C
Zhou
Chun
C
Zeng
Li
L
Gu
Shaohua
S
Ying
Kang
K
Xie
Yi
Y
Mao
Yumin
Y
D042964:Acyl-CoA Dehydrogenase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D044944:Acyl-CoA Dehydrogenases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D002881:Chromosomes, Human, Pair 12; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D015723:Gene Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D044944:Acyl-CoA Dehydrogenases; C499845:ACAD10 protein, human; D042964:Acyl-CoA Dehydrogenase; D010100:Oxygen