A pre-tRNA carrying intron features typical of Archaea is spliced in yeast.
Archaeal pre-tRNAs are characterized by the presence of the bulge-helix-bulge (BHB) structure in the intron stem-and-loop region. A chimeric pre-tRNA was constructed bearing an intron of the archaeal type and the mature domain of the Saccharomyces cerevisiae suppressor SUP4 tRNA(Tyr). This pre-tRNA(ArchEuka) is correctly cleaved in several cell-free extracts and by purified splicing endonucleases. It is also cleaved and ligated in S. cerevisiae cells, providing efficient suppression of nonsense mutations in various genes.
Di Segni
Gianfranco
G
Istituto di Biologia Cellulare, CNR, Via E. Ramarini 32, 00016 Monterotondo Scalo (Rome) 00016, Italy.
Borghese
Lodovica
L
Sebastiani
Silvia
S
Tocchini-Valentini
Glauco P
GP
D001483:Base Sequence; D002678:Chimera / Q000235:genetics; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012326:RNA Splicing; D019642:RNA, Archaeal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012365:RNA, Transfer, Tyr / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013489:Suppression, Genetic
D012322:RNA Precursors; D019642:RNA, Archaeal; D012365:RNA, Transfer, Tyr; C030449:pre-tRNA(Tyr)