title: The DNA methylation events in normal and cloned rabbit embryos.
abstract: To study the DNA methylation events in normal and cloned rabbit embryos, we investigated the methylation status of a satellite sequence and the promoter region of a single-copy gene using bisulfite-sequencing technology. During normal rabbit embryo development, both sequences maintained hypermethylation status until the 8- to 16-cell stage when progressive demethylation took place. In cloned embryos, the single-copy gene promoter sequence was rapidly demethylated and precociously de novo methylated, while the satellite sequence maintained the donor-type methylation status in all examined stages. Our results indicate that unique sequences as well as satellite sequences may have aberrant methylation patterns in cloned embryos.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Graduate School, The Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Liu
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Chen
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D001483:Base Sequence; D019976:Cloning, Organism / Q000009:adverse effects; D019175:DNA Methylation; D004276:DNA, Satellite / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics / Q000378:metabolism; D011817:Rabbits / Q000196:embryology* / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D013447:Sulfites / Q000378:metabolism
chemical_list: D004276:DNA, Satellite; D037662:Pulmonary Surfactant-Associated Protein A; D013447:Sulfites; C042345:hydrogen sulfite