title: Polyelectrolyte multilayers functionalized by a synthetic analogue of an anti-inflammatory peptide, alpha-MSH, for coating a tracheal prosthesis.
abstract: Polyelectrolyte multilayer films made of poly (L-lysine) (PLL) and poly (L-glutamic acid) (PGA) have been functionalized by covalent binding of a synthetic analogue of the anti-inflammatory peptide, alpha-melanocyte-stimulating hormone (alpha-MSH) to PGA to create biologically active coatings for tracheal prostheses. The morphology and in vivo stability of the films were investigated by atomic force microscopy and confocal laser scanning microscopy, respectively. For the in vivo evaluation, 87 rats were implanted and examined for a period superior to 3 months. Histological analysis, performed 1 month after implantation, showed a fibroblast colonization of the periprosthetic side and a respiratory epithelium type on the endoluminal side of the implant for all the polyelectrolyte coatings tested. However, for prostheses modified by PGA ending multilayer films, a more regular and less obstructive cell layer was observed on the endoluminal side compared to those modified by PLL ending films. Systemic anti-inflammatory IL-10 production was only detected in rats implanted with prostheses functionalized by alpha-MSH, demonstrating, in vivo, the anti-inflammatory activity of the embedded peptide into multilayer architectures.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Hautepierre Hospital, Strasbourg, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Schultz
authors.forename: Dominique
authors.initials: D
authors.lastname: Vautier
authors.forename: Ludovic
authors.initials: L
authors.lastname: Richert
authors.forename: Nadia
authors.initials: N
authors.lastname: Jessel
authors.forename: Youssef
authors.initials: Y
authors.lastname: Haikel
authors.forename: Pierre
authors.initials: P
authors.lastname: Schaaf
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Voegel
authors.forename: Joelle
authors.initials: J
authors.lastname: Ogier
authors.forename: Christian
authors.initials: C
authors.lastname: Debry
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry; D004343:Drug Implants / Q000008:administration & dosage / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry; D019544:Equipment Failure Analysis; D019344:Lactic Acid / Q000737:chemistry*; D007825:Laryngectomy; D007831:Larynx, Artificial / Q000009:adverse effects*; D008297:Male; D008422:Materials Testing; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011474:Prosthesis Design; D011475:Prosthesis Failure; D016459:Prosthesis-Related Infections / Q000276:immunology / Q000473:pathology* / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000187:drug effects* / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D016896:Treatment Outcome; D000521:alpha-MSH / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D020099:Coated Materials, Biocompatible; D004343:Drug Implants; D004573:Electrolytes; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000521:alpha-MSH