Steroid epimers studied by different mass spectrometric methods.
The combined use of different mass spectrometric ionization methods and MS/MS techniques provide the possibility to differentiate between stereoisomers or epimers. In this paper the mass spectral decomposition of 11alpha- and 11beta-substituted estrans was studied. Distinctive stereochemical effects have been observed in their fast atom bombardment product ion spectra. In the electron ionisation (EI) mode, the 5alpha- and 5beta-hydroxylated compounds showed significant differences in the abundance of water elimination. Mass spectrometry has proved to be an effective tool when stereoisomer steroids are compared.
Mák
Marianna
M
Gedeon Richter Ltd., Budapest, P.O. Box 27, H-1475, Hungary. m.mak@richter.hu
Francsics-Czinege
Erzsébet
E
Tuba
Zoltán
Z
D004962:Estranes / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D013237:Stereoisomerism
D004962:Estranes