Determination of linezolid in plasma and bronchoalveolar lavage by high-performance liquid chromatography with ultraviolet detection using a fully automated extraction method.
The aim of this study was to develop a specific and sensitive high-performance liquid chromatographic assay for the determination of linezolid in human plasma, and bronchoalveolar lavage. The sample extraction was based on a fully automated solid-phase extraction with an OASIS HLB cartridge. The method used ultraviolet detection set at a wavelength of 254 nm and a separation with a Zorbax Eclipse XDB C8 column. The assay has been found linear over the concentration range 0.02-30 microg/ml and 0.04-30 microg/ml for linezolid, respectively, in plasma and bronchoalveolar lavage. It provided good validation data for accuracy and precision (CV <4.64% and 5.08%, accuracy in the range 96.93-102.67% and 97.33-105.67%, respectively, for intra- and inter-day). The assay will be applied to determine the penetration of linezolid in human bronchoalveolar lavage during pharmacokinetic steady-state.
Toutain
Jérôme
J
Clinical Pharmacokinetic Department, Victor Segalen Bordeaux 2 University, Bordeaux, France.
Boselli
Emmanuel
E
Djabarouti
Sarah
S
Allaouchiche
Bernard
B
Xuereb
Fabien
F
Bernadou
Jean-Marc
JM
Ba
Boubakar
B
Saux
Marie-Claude
MC
Breilh
Dominique
D
D000081:Acetamides / Q000097:blood* / Q000493:pharmacokinetics; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D001331:Automation; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D000069349:Linezolid; D023303:Oxazolidinones / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D000069349:Linezolid