Identification of mutations in proviral long terminal repeats of HIV type 1-infected subjects naive to drug therapy.
Human immunodeficiency virus type 1 (HIV-1) proviral DNA sequences in the 5' long terminal repeat (LTR) were examined among 28 drug-naive individuals. Twenty-four subjects had highly conserved LTR sequences, however, more significant changes were observed in the remaining four LTR sequences. These included a 9-bp deletion preceding the NF kappa B elements and a duplication of the RBF-2 motif. A higher overall frequency of mutations within the LTR occurred within NFAT-1 and Sp-1 sequences. Importantly, a novel 16-bp deletion was found in the distal NFAT-1 site.
Copeland
Karen F T
KF
Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada K1H 8L6. kcopeland@ohri.ca
Chen
Zhaoxia
Z
Fiebig
Marnie
M
Ni
Lisa
L
Savoy
Sherie
S
Smaill
Fiona M
FM
Rosenthal
Kenneth L
KL
Kim
John E
JE
D001483:Base Sequence; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011533:Proviruses / Q000235:genetics*; D017422:Sequence Analysis, DNA