title: RNA fingerprinting of microbial community in the rhizosphere soil of grain legumes.
abstract: Microbial structural and expression profiles of the rhizospheres of three legumes, faba beans, peas and white lupin, were compared by RNA-arbitrarily primed PCR technique. Two different primers, M13 reverse and 10-mer primers, were used in the amplification and products resolved on non-denaturing polyacrylamide gel. With both DNA and RNA profiles Lupinus and Pisum rhizospheres were more similar to each other than to Vicia rhizosphere. The RAP-PCR products were also dot blotted and probed for bacterial peptidase transcripts. Plant-dependent rhizosphere effect was evident by the marked absence of transcripts for bacterial neutral metallopeptidase in Lupinus rhizosphere. The results of dot blot were further confirmed by RT-PCR for the expression of bacterial neutral metallopeptidase in the three rhizospheres.
authors.affiliation: GSF-National Research Center for Environment and Health, Institute of Soil Ecology, Neuherberg, Germany. shilpi.sharma@gsf.de
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: M K
authors.initials: MK
authors.lastname: Aneja
authors.forename: J
authors.initials: J
authors.lastname: Mayer
authors.forename: M
authors.initials: M
authors.lastname: Schloter
authors.forename: J C
authors.initials: JC
authors.lastname: Munch
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D016172:DNA Fingerprinting; D018076:DNA, Complementary; D007887:Fabaceae / Q000382:microbiology*; D031297:Lupinus / Q000382:microbiology; D045726:Metalloproteases / Q000032:analysis / Q000235:genetics*; D018532:Pisum sativum / Q000382:microbiology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012988:Soil Microbiology; D014158:Transcription, Genetic; D031307:Vicia faba / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012313:RNA; D045726:Metalloproteases