title: The Ste20-like kinase misshapen functions together with Bicaudal-D and dynein in driving nuclear migration in the developing drosophila eye.
abstract: Nuclear translocation, driven by the motility apparatus consisting of the cytoplasmic dynein motor and microtubules, is essential for cell migration during embryonic development. Bicaudal-D (Bic-D), an evolutionarily conserved dynein-interacting protein, is required for developmental control of nuclear migration in Drosophila. Nothing is known about the signaling events that coordinate the function of Bic-D and dynein during development. Here, we show that Misshapen (Msn), the fly homolog of the vertebrate Nck-interacting kinase is a component of a novel signaling pathway that regulates photoreceptor (R-cell) nuclear migration in the developing Drosophila compound eye. Msn, like Bic-D, is required for the apical migration of differentiating R-cell precursor nuclei. msn displays strong genetic interaction with Bic-D. Biochemical studies demonstrate that Msn increases the phosphorylation of Bic-D, which appears to be necessary for the apical accumulation of both Bic-D and dynein in developing R-cell precursor cells. We propose that Msn functions together with Bic-D to regulate the apical localization of dynein in generating directed nuclear migration within differentiating R-cell precursor cells.
authors.affiliation: McGill Centre for Research in Neuroscience, and Department of Neurology and Neurosurgery, McGill University Health Centre, 1650 Cedar Avenue, Montreal, Que., Canada.
authors.forename: Tarek
authors.initials: T
authors.lastname: Houalla
authors.forename: Dac
authors.initials: D
authors.lastname: Hien Vuong
authors.forename: Wenjing
authors.initials: W
authors.lastname: Ruan
authors.forename: Beat
authors.initials: B
authors.lastname: Suter
authors.forename: Yong
authors.initials: Y
authors.lastname: Rao
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D002467:Cell Nucleus; D004330:Drosophila / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004398:Dyneins / Q000235:genetics* / Q000378:metabolism; D005123:Eye / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C061998:BicD protein, Drosophila; D029721:Drosophila Proteins; C113875:msn protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D004398:Dyneins