Adenosine transport in HPRT deficient lymphocytes from Lesch-Nyhan disease patients.
We have analysed adenosine transport and [3H] NBTI binding in peripheral blood lymphocytes obtained from Lesch-Nyhan patients, in basal conditions and following 24 h incubation with hypoxanthine. We found that adenosine transport and [3H] NBTI binding were significantly decreased in PBL-LN with respect to PBL-C in basal conditions. Following 25 microM hypoxanthine incubation, adenosine transport is decreased in PBL-LN with respect to basal transport, however, [3H] NBTI binding in PBL-LN was not decreased following hypoxanthine incubation.
Torres
R J
RJ
Biochemistry Laboratory, La Paz Hospital, Madrid, Spain.
DeAntonio
I
I
Prior
C
C
Puig
J G
JG
D000241:Adenosine / Q000378:metabolism* / Q000494:pharmacology; D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019271:Hypoxanthine / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency* / Q000235:genetics*; D007700:Kinetics; D007926:Lesch-Nyhan Syndrome / Q000097:blood* / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology*; D009705:Nucleosides / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D013997:Time Factors
D009705:Nucleosides; D009711:Nucleotides; D019271:Hypoxanthine; D007041:Hypoxanthine Phosphoribosyltransferase; D000241:Adenosine