BRE enhances in vivo growth of tumor cells.
Human BRE, a death receptor-associating intracellular protein, attenuates apoptotic response of human and mouse tumor cell lines to death receptor stimuli in vitro. In this report, we addressed whether the in vitro antiapoptotic effect of BRE could impact on tumor growth in vivo. We have shown that the mouse Lewis lung carcinoma D122 stable transfectants of human BRE expression vector developed into local tumor significantly faster than the stable transfectants of empty vector and parental D122, in both the syngeneic C57BL/6 host and nude mice. In vitro growth of the BRE stable transfectants was, however, not accelerated. No significant difference in metastasis between the transfectants and the parental D122 was detected. Thus, overexpression of BRE promotes local tumor growth but not metastasis. We conclude that the enhanced tumor growth is more likely due to the antiapoptotic activity of BRE than any direct effect of the protein on cell proliferation.
Chan
Ben Chung-Lap
BC
Clinical Immunology Unit and Sir Y.K. Pao Centre for Cancer, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China.
Li
Qing
Q
Chow
Stephanie Ka-Yee
SK
Ching
Arthur Kar-Keung
AK
Liew
Choong Tsek
CT
Lim
Pak-Leong
PL
Lee
Kenneth Ka-Ho
KK
Chan
John Yeuk-Hon
JY
Chui
Yiu-Loon
YL
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*
C091514:BABAM2 protein, human; D009419:Nerve Tissue Proteins