title: The effect of Eriobotrya japonica seed extract on oxidative stress in adriamycin-induced nephropathy in rats.
abstract: Eriobotrya japonica has been used as a medicinal plant for a long time, and its leaves are known to have many physiological actions such as anti-inflammatory, antitussive, and expectoran. In contrast, Eriobotrya japonica seeds are only known to contain amygdalin, and almost no investigations of its pharmacological action have been performed. Moreover, some anticancer agents such as adriamycin cause renal disorders as an adverse effect, and the mechanism of the adverse effect is considered to involve oxidative stress. We have reported that Eriobotrya japonica seed extract has an inhibitory effect on liver disorders. In this study, we prepared a 70% ethanol extract of Eriobotrya japonica seeds and administered the extract to rats with renal disorder induced by a single administration of 7 mg/kg body weight adriamycin, and investigated the usefulness of the extract. Increases in indices of renal function, plasma urea nitrogen, were significantly inhibited in rats treated with the Eriobotrya japonica extract compared to rats treated with tap water. In addition, the renal tissue level of reduced glutathione was significantly high in rats that ingested the extract, while the lipid peroxide levels in plasma and renal tissue were significantly low. However, no effect on renal tissue antioxidative enzymes was observed, suggesting that Eriobotrya japonica seed extract has direct antioxidative action. Based on these findings, Eriobotrya japonica seed extract may be effective in reducing the oxidative stress of adriamycin-induced renal disorder. Therefore, ingestion of Eriobotrya japonica seed extract may contribute to a reduction of the adverse effects of adriamycin.
authors.affiliation: Department of Pharmacy, Kochi Medical School Hospital, Japan. hamadaa@med.kochi-u.ac.jp
authors.forename: Atsuhide
authors.initials: A
authors.lastname: Hamada
authors.forename: Saburo
authors.initials: S
authors.lastname: Yoshioka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takuma
authors.forename: Junko
authors.initials: J
authors.lastname: Yokota
authors.forename: Tailine
authors.initials: T
authors.lastname: Cui
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kusunose
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Miyamura
authors.forename: Shojiro
authors.initials: S
authors.lastname: Kyotani
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nishioka
mesh_terms: D000818:Animals; D004317:Doxorubicin / Q000633:toxicity*; D031983:Eriobotrya; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds
chemical_list: D010936:Plant Extracts; D004317:Doxorubicin