Crystallization and preliminary crystallographic analysis of the 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10.
CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 A, alpha = beta = gamma = 90 degrees . The crystals diffracted to a maximum resolution of 1.9 A and gave a data set with an overall R(merge) of 5.7% and a completeness of 98.6%. The V(M) value was 2.52 A(3) Da(-1), which indicated a solvent content of 51.2%.
Iwata
Kenichi
K
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Noguchi
Haruko
H
Usami
Yusuke
Y
Nam
Jeong Won
JW
Fujimoto
Zui
Z
Mizuno
Hiroshi
H
Habe
Hiroshi
H
Yamane
Hisakazu
H
Omori
Toshio
T
Nojiri
Hideaki
H
D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D049308:Dioxygenases / Q000737:chemistry*; D006860:Hydrogen Bonding; D008956:Models, Chemical; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D011549:Pseudomonas / Q000201:enzymology*; D012997:Solvents; D017356:Synchrotrons; D014965:X-Rays
D012997:Solvents; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase