Genomic deletions of the Drosophila melanogaster Hsp70 genes.
Homologous recombination can produce directed mutations in the genomes of a number of model organisms, including Drosophila melanogaster. One of the most useful applications has been to delete target genes to generate null alleles. In Drosophila, specific gene deletions have not yet been produced by this method. To test whether such deletions could be produced by homologous recombination in D. melanogaster we set out to delete the Hsp70 genes. Six nearly identical copies of this gene, encoding the major heat-shock protein in Drosophila, are found at two separate but closely linked loci. This arrangement has thwarted standard genetic approaches to generate an Hsp70-null fly, making this an ideal test of gene targeting. In this study, ends-out targeting was used to generate specific deletions of all Hsp70 genes, including one deletion that spanned approximately 47 kb. The Hsp70-null flies are viable and fertile. The results show that genomic deletions of varied sizes can be readily generated by homologous recombination in Drosophila.
Gong
Wei J
WJ
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
Golic
Kent G
KG
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D017353:Gene Deletion; D018390:Gene Targeting; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*
D018840:HSP70 Heat-Shock Proteins