Rice bran extract prevents the elevation of plasma peroxylipid in KKAy diabetic mice.
Oxidative stress is now considered to be a key factor in the development of diabetes and its complications. In this study, we examined the anti-oxidative effects of a crude lipophilic rice bran extract, Ricetrienol, which contains alpha-tocopherol, tocotrienol and phytosterol, in obese diabetic KKAy mice. We used KKAy mice fed a normal diet (DM group) or a diet including 0.1% Ricetrienol (RT group), and non-diabetic C57BL mice (C group). After 6 weeks, body weight, HbA1c, plasma glucose, lipids, peroxylipid (malonedialdehyde, MDA), alpha-tocopherol and glutathione peroxidase 1 (GPx) mRNA expression in the kidney were measured. At 1 week and at the end of the experimental period, urine 8-isoprostane and 8-hydroxy deoxyguanosine (8-OHdG) were also measured. Ricetrienol administration did not affect hyperglycemia, body weight or hyperlipidemia. Plasma MDA, urine 8-isoprostane and 8-OHdG in the DM group were significantly increased compared with the C group and the elevation of plasma MDA was significantly suppressed by 0.1% Ricetrienol. GPx mRNA expression was significantly increased in the RT group when compared with the C group. Plasma alpha-tocopherol in the RT group was significantly higher than that in the DM group. These findings suggest that Ricetrienol exerts a protective effect against oxidative damage in diabetes mellitus.
Kanaya
Yumi
Y
Tsuno Food Industrial Co. Ltd., Kimiidera 811-1, Wakayama, P.O. 641-8509, Japan.
Doi
Takuya
T
Sasaki
Hideyuki
H
Fujita
Atsuyo
A
Matsuno
Shohei
S
Okamoto
Kunihisa
K
Nakano
Yuuko
Y
Tsujiwaki
Satomi
S
Furuta
Hiroto
H
Nishi
Masahiro
M
Tsuno
Takuo
T
Taniguchi
Hisaji
H
Nanjo
Kishio
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000652:urine; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000235:genetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D012275:Oryza; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D012016:Reference Values; D024502:alpha-Tocopherol / Q000494:pharmacology
D000975:Antioxidants; D008055:Lipids; D010936:Plant Extracts; D012333:RNA, Messenger; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D003849:Deoxyguanosine; D024502:alpha-Tocopherol