Lentiviral vectors pseudotyped with envelope glycoproteins derived from human parainfluenza virus type 3.
We describe the generation of lentiviruses pseudotyped with human parainfluenza type 3 envelope (HPIV3) glycoproteins. Lentivirus particles, expressed in 293T/17 cells, incorporate HPIV3 hemagglutinin-neuraminidase (HN) and fusion (F) proteins into their lipid bilayers and are able to transduce human kidney epithelial cells and polarized MDCK cells. Neuraminidase, AZT, and anti-HPIV3 antisera block transduction, which is consistent with lentiviral-mediated transduction via sialated receptors for HPIV3. Our findings show that HPIV3 pseudotyped lentiviruses can be formed and may have a number of useful properties for human gene transfer.
Jung
Cindy
C
The Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, Georgia 30332-0535, USA.
Grzybowski
Bradley N
BN
Tong
Suxiang
S
Cheng
LiTeng
L
Compans
Richard W
RW
Le Doux
Joseph M
JM
D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics*; D015707:HN Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics*; D010224:Parainfluenza Virus 3, Human / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014161:Transduction, Genetic / Q000379:methods*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D015707:HN Protein; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; C072983:F protein, parainfluenza virus 3