[Effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro].
OBJECTIVE
To study the effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro.


METHODS
MTT and PNPP were used separately to observe the proliferation and the activity of ALP of osteoblasts cultured in vitro with different concentration genistein for different incubation periods. Protein was measured by Lowry method.


RESULTS
It was found that genistein had the effects on promoting proliferation of osteoblasts cultured for 24 h and 48 h at concentration ranging from 1 x 10(-9) mol/L to 1 x 0(-5) mol/L. The activity of ALP was stimulated by genistein at concentration ranging from 1 x 10(-7) mol/L to 1 x 10(-5) mol/L after 72 h incubation.


CONCLUSIONS
Genistein has the effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro.
Wang
Jianhua
J
Institute of Combined Traditional Chinese and Western Medicine, Hebei Medical University, Shijiazhuang 050017.
Kong
Dejuan
D
Li
En
E
Tong
Xiaoxu
X
Chen
Yongchun
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D007529:Isoflavones / Q000737:chemistry; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D019833:Genistein; D000469:Alkaline Phosphatase