A phage display system with unnatural amino acids.
We report a general method to display peptide-containing unnatural amino acids on filamentous M13 phage. Five distinct unnatural amino acids were site-specifically incorporated at the N-terminal of the M13 phage minor coat protein pIII. Phages that contain p-azidophenylalanine can undergo a highly specific azide-alkyne [3 + 2] cycloaddition reaction with an alkyne-derivatized fluorophore. The generalization of phage display to include unnatural amino acids should significantly increase the scope of phage display technology.
Tian
Feng
F
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Tsao
Meng-Lin
ML
Schultz
Peter G
PG
D000596:Amino Acids / Q000235:genetics* / Q000378:metabolism*; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D017017:Methanococcus / Q000235:genetics / Q000378:metabolism; D010452:Peptide Biosynthesis / Q000235:genetics*; D019151:Peptide Library; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism
D000596:Amino Acids; D036022:Capsid Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D019151:Peptide Library; D014760:Viral Fusion Proteins; C452700:streptavidin-binding peptide