title: Chronic systemic hypoxia causes intra-retinal angiogenesis.
abstract: Retinal hypoxia occurs in many conditions that cause vascular disease in the eye and is an important stimulus to new vessel formation. However, the adult retina can also become hypoxic when there is systemic hypoxaemia such as occurs in chronic lung diseases, congenital cardiac disease and residence at high altitude. Little is known about the adaptive responses of the retinal vasculature in such circumstances. Previous research in the retinopathy of prematurity model may not apply to the adult tissue given the different mechanisms controlling angiogenesis in developing and mature circulations. We tested the hypothesis that chronic systemic hypoxia leads to angiogenesis in the adult retinal circulation, in the absence of pre-existing vascular disease. Adult male Sprague-Dawley rats (n=9) were exposed to a fraction of inspired oxygen of 0.10 for 2 weeks while control animals (n=10) were exposed to room air. Stereological techniques were used to quantify the vascular volume, endothelial surface area and the total number of branch points of all blood vessels in the superficial retinal vascular plexus. The mean volume and endothelial surface area of these vessels were significantly greater in the hypoxic than in the control group. The mean number of blood vessel branch points was also significantly greater in the hypoxic group. Our findings demonstrate that chronic systemic hypoxia, in the absence of other pathological processes, causes angiogenesis in the adult rat retina and provide an in vivo model for investigating this important process in the adult retina, in particular pathways specific to this tissue.
authors.affiliation: Department of Human Anatomy and Physiology, University College Dublin Medical School, Ireland.
authors.forename: Alex J
authors.initials: AJ
authors.lastname: Shortt
authors.forename: Katherine
authors.initials: K
authors.lastname: Howell
authors.forename: Colm
authors.initials: C
authors.lastname: O'Brien
authors.forename: Paul
authors.initials: P
authors.lastname: McLoughlin
mesh_terms: D000818:Animals; D002908:Chronic Disease; D000860:Hypoxia / Q000473:pathology*; D008297:Male; D009389:Neovascularization, Pathologic / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012171:Retinal Vessels / Q000473:pathology*