title: A shortened dengue IgM capture ELISA using simultaneous incubation of antigen and peroxidase-labeled monoclonal antibody.
abstract: A shortened IgM capture ELISA for the detection of dengue IgM antibodies using simultaneous incubation of antigen and peroxidase-labeled monoclonal antibody was described. The shortened two-step assay was compared with the four-step IgM capture ELISA on sera from dengue patients confirmed by the hemagglutination inhibition (HI) test. When paired acute and convalescent sera were tested, the shortened ELISA showed 100% agreement with HI results. It detected dengue IgM antibodies in the acute sera of 66% of patients with a primary dengue infection, 60% of patients with a secondary infection, and 98% of patients with a presumptive secondary infection. When the results of 151 dengue patients were combined, 75% of the acute sera were positive by the shortened IgM capture ELISA.
authors.affiliation: Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore City, Singapore 0511.
authors.forename: C F
authors.initials: CF
authors.lastname: Chong
authors.forename: B L
authors.initials: BL
authors.lastname: Ngoh
authors.forename: H C
authors.initials: HC
authors.lastname: Tan
authors.forename: E H
authors.initials: EH
authors.lastname: Yap
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: L
authors.initials: L
authors.lastname: Chan
authors.forename: Y C
authors.initials: YC
authors.lastname: Chan