title: The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases.
abstract: ATP- and NAD(+)-dependent DNA ligases, ATP-dependent RNA ligases and GTP-dependent mRNA capping enzymes comprise a superfamily of proteins that catalyze nucleotidyl transfer to polynucleotide 5' ends via covalent enzyme-(lysyl-N)-NMP intermediates. The superfamily is defined by five peptide motifs that line the nucleotide-binding pocket and contribute amino acid sidechains essential for catalysis. Early crystal structures revealed a shared core tertiary structure for DNA ligases and capping enzymes, which are composed minimally of a nucleotidyltransferase domain fused to a distal OB-fold domain. Recent structures of viral and bacterial DNA ligases, and a fungal mRNA capping enzyme underscore how the substrate-binding and chemical steps of the ligation and capping pathways are coordinated with large rearrangements of the component protein domains and with remodeling of the atomic contacts between the enzyme and the nucleotide at the active site. The first crystal structure of an RNA ligase suggests that contemporary DNA ligases, RNA ligases and RNA capping enzymes evolved by fusion of ancillary effector domains to an ancestral catalytic module involved in RNA repair.
authors.affiliation: Molecular Biology and Structural Biology Programs, Sloan-Kettering Institute for Cancer Research, New York, New York 10021, USA.
authors.forename: Stewart
authors.initials: S
authors.lastname: Shuman
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Lima
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites; D004789:Enzyme Activation; D008954:Models, Biological; D008956:Models, Chemical; D008958:Models, Molecular; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000378:metabolism*; D011118:Polynucleotide Ligases / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012315:RNA Caps / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D012315:RNA Caps; D009713:Nucleotidyltransferases; D011118:Polynucleotide Ligases