title: The levels of cockroach allergen in relation to cockroach species and allergic diseases in Thai patients.
abstract: Recently, cockroaches have been established as the second most Important allergen, producing allergic diseases, especially in low socioeconomic populations. In Thailand, about 44-61% of atopic patients were positive to cockroach extract by a skin-prick test. This study examined cockroach allergen levels in relation to cockroach species and allergic diseases in the houses of cockroach-sensitive patients. Sixty households of allergic patients in the Bangkok metropolitan area were surveyed using open- and closed-ended questionnaires. Cockroaches were collected using commercial cockroach traps, while dust samples were obtained from the bedrooms, kitchens and living rooms of the houses using a vacuum cleaner. The cockroaches were counted and their species Identified. The levels of cockroach allergens were determined by specific monoclonal antibodies using a monoclonal antibody-polyclonal antibody based sandwich ELISA kit. Six cockroach species were Identified: Periplaneta americana (American cockroach, 72.15%), Supella longlpalpa (2.75%, found in only one house), Periplaneta brunnea (0.78%), Periplaneta australaslae (0.78%), Neostylopyga rhombifolla (0.78%), Blattella germanica (German cockroach, 0.39%) and nymphs (22.35%). Allergens of the predominant species, P. americana, were detectable in all homes studied, with the highest levels in the kitchen areas. The range of allergen levels in house dust varied from 0.40-162.00 microg per g of dust. The median and mean allergen levels in kitchen dust were 59.16 microg and 62.80 microg per g of dust, respectively, while the median allergen level in bedroom dust was only 15.90 microg per g of dust. The German cockroach allergen (Bla g 2) was undetectable in any of the houses.
  
  
  CONCLUSIONS
  P. americana was the most common cockroach and may be the species causing allergic diseases, especially asthma, in Thailand, which differs from the USA and Europe
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tungtrongchitr
authors.forename: Nitat
authors.initials: N
authors.lastname: Sookrung
authors.forename: Nantiya
authors.initials: N
authors.lastname: Munkong
authors.forename: Vanna
authors.initials: V
authors.lastname: Mahakittikun
authors.forename: Pisith
authors.initials: P
authors.lastname: Chinabut
authors.forename: Wanpen
authors.initials: W
authors.lastname: Chaicumpa
authors.forename: Chaweewan
authors.initials: C
authors.lastname: Bunnag
authors.forename: Pakit
authors.initials: P
authors.lastname: Vichyanond
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000818:Animals; D003058:Cockroaches / Q000145:classification / Q000276:immunology*; D004391:Dust / Q000276:immunology*; D004781:Environmental Exposure / Q000009:adverse effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000453:epidemiology* / Q000209:etiology; D019476:Insect Proteins / Q000032:analysis* / Q000276:immunology; D013785:Thailand
chemical_list: D000485:Allergens; D004391:Dust; D019476:Insect Proteins