In vitro effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles.
OBJECTIVE
To test the effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles during prolonged culture period.


METHODS
Donated ovarian biopsies from 16 women undergoing gynecological laparoscopy were cut into slices and cultured in parallel for 1, 2, or 3 weeks in the presence and the absence of 0.5 mM 8-bromo-cAMP. The developmental stages, sizes, and viability of the follicles were recorded from histological sections of all samples.


RESULTS
On day 14, cortical slices cultured with 8-bromo-cAMP showed a significantly higher proportion of secondary follicles (50.0% vs. 20.0%) and a lower proportion of primordial follicles (9.7% vs. 26.7%) when compared with those cultured without 8-bromo-cAMP. On day 21, the proportion of viable follicles in cortical slices with 8-bromo-cAMP treatment was significantly higher than that without 8-bromo-cAMP treatment (79.6% vs. 55.2%).


CONCLUSIONS
CyclicAMP promoted folliculogenesis and follicle survival during 14-21 days' culture of human ovarian cortical slices.
Zhang
Pu
P
Department of Clinical Science, Division of Obstetrics and Gynecology, Karolinska Institute, Huddinge University Hospital, S-141 86 Stockholm, Sweden.
Louhio
Henna
H
Tuuri
Timo
T
Sjöberg
Jari
J
Hreinsson
Julius
J
Telfer
Evelyn E
EE
Hovatta
Outi
O
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
D015124:8-Bromo Cyclic Adenosine Monophosphate