title: [NF-kappaB expression in lung tissue of acute lung injury rat model and the influence by antioxidant N-acetylcysteine].
abstract: OBJECTIVE
  To observe the NF-kappaB expression in the lung tissue of LPS-induced acute lung injury(ALI) rat model and the influence of N-acetylcysteine (NAC) on NF-kappaB expression.
  
  
  METHODS
  The expression of NF-kappaB in lung tissue in ALI rat model and the influence of NAC on NF-kappaB expression were detected by immunohistochemical (ABC) staining and Western blot.
  
  
  RESULTS
  There were a small amount of sporadic NF-kappaB cells in airway epithelium and interstitium in normal control group. In contrast, nuclear NF-kappaB expression-positive cells increased obviously in airway mucosa, lung interestium, alveolar cavity and vascular wall of ALI rats. NF-kappaB(+) cells were mainly airway mucosa epithelial cells, infiltrating inflammatory cells, alveolar epithelial cells, and vascular endothelial cells. The NF-kappaB expression-positive cells in NAC therapy group notably decreased compared with ALI group and control group(P<0.01). Western blot analysis showed that the expression of NF-kappaB was different at various time points, reaching the peak at 3 h and then decreased (P<0.01) after LPS induced lung injury.
  
  
  CONCLUSIONS
  In LPS induced acute lung injury rat model, the NF-kappaB nuclear expression increased obviously in airway mucosa, lung interestium and alveolar cavity. Most cells in lung tissue participated in the activation of NF-kappaB. NAC could alleviate inflammation by inhibiting activation of NF-kappaB.
authors.affiliation: Department of Respiratory Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. Zhangjian1970@hotmail.com
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Sheng-qing
authors.initials: SQ
authors.lastname: Li
authors.forename: Huan-zhang
authors.initials: HZ
authors.lastname: Li
authors.forename: Hao-wen
authors.initials: HW
authors.lastname: Qi
authors.forename: Chang-gui
authors.initials: CG
authors.lastname: Wu
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D000111:Acetylcysteine