Functional interaction in establishment of ribosomal integrity between small subunit protein rpS6 and translational regulator rpL10/Grc5p.
Functional ribosomes synthesize proteins in all living cells and are composed of two labile associated subunits, which are made of rRNA and ribosomal proteins. The rRNA of the small 40S subunit (SSU) of the functional eukaryotic 80S ribosome decodes the mRNA molecule and the large 60S subunit (LSU) rRNA catalyzes protein synthesis. Recent fine structure determinations of the ribosome renewed interest in the role of ribosomal proteins in modulation of the core ribosomal functions. RpL10/Grc5p is a component of the LSU and is a multifunctional translational regulator, operating in 60S subunit biogenesis, 60S subunit export and 60S subunit joining with the 40S subunit. Here, we report that rpL10/Grc5p functionally interacts with the nuclear export factor Nmd3p in modulation of the cellular polysome complement and with the small subunit protein rpS6 in subunit joining and differential protein expression.
Pachler
Karin
K
Department of Cell Biology, Paris Lodron University Salzburg, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria.
Karl
Thomas
T
Kolmann
Kerstin
K
Mehlmer
Norbert
N
Eder
Michaela
M
Loeffler
Michael
M
Oender
Kamil
K
Hochleitner
Elisabeth O
EO
Lottspeich
Friedrich
F
Bresgen
Nikolaus
N
Richter
Klaus
K
Breitenbach
Michael
M
Koller
Lore
L
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D000080882:Ribosomal Protein L10; D038601:Ribosomal Protein S6 / Q000235:genetics / Q000502:physiology*; D012269:Ribosomal Proteins / Q000235:genetics / Q000502:physiology*; D012270:Ribosomes / Q000235:genetics / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015003:Yeasts / Q000235:genetics / Q000502:physiology*
D004271:DNA, Fungal; D005656:Fungal Proteins; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins