title: Molecular bases for sensitivity to acetyl-coenzyme A carboxylase inhibitors in black-grass.
abstract: In grasses, residues homologous to residues Ile-1,781 and Ile-2,041 in the carboxyl-transferase (CT) domain of the chloroplastic acetyl-coenzyme A (CoA) carboxylase (ACCase) from the grass weed black-grass (Alopecurus myosuroides [Huds.]) are critical determinants for sensitivity to two classes of ACCase inhibitors, aryloxyphenoxypropionates (APPs) and cyclohexanediones. Using natural mutants of black-grass, we demonstrated through a molecular, biological, and biochemical approach that residues Trp-2,027, Asp-2,078, and Gly-2,096 are also involved in sensitivity to ACCase inhibitors. In addition, residues Trp-2,027 and Asp-2,078 are very likely involved in CT activity. Using three-dimensional modeling, we found that the side chains of the five residues are adjacent, located at the surface of the inside of the cavity of the CT active site, in the vicinity of the binding site for APPs. Residues 1,781 and 2,078 are involved in sensitivity to both APPs and cyclohexanediones, whereas residues 2,027, 2,041, and 2,096 are involved in sensitivity to APPs only. This suggests that the binding sites for these two classes of compounds are overlapping, although distinct. Comparison of three-dimensional models for black-grass wild-type and mutant CTs and for CTs from organisms with contrasted sensitivity to ACCase inhibitors suggested that inhibitors fitting into the cavity of the CT active site of the chloroplastic ACCase from grasses to reach their active sites may be tight. The three-dimensional shape of this cavity is thus likely of high importance for the efficacy of ACCase inhibitors.
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche, Biologie et Gestion des Adventices, F-21065 Dijon cedex, France. delye@dijon.inra.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Délye
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Séverine
authors.initials: S
authors.lastname: Michel
authors.forename: Annick
authors.initials: A
authors.lastname: Matéjicek
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Powles
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D000595:Amino Acid Sequence; D001665:Binding Sites; D004305:Dose-Response Relationship, Drug; D006540:Herbicides / Q000494:pharmacology*; D008958:Models, Molecular; D006109:Poaceae / Q000201:enzymology*; D017434:Protein Structure, Tertiary
chemical_list: D006540:Herbicides; D000103:Acetyl-CoA Carboxylase