title: BRE enhances in vivo growth of tumor cells.
abstract: Human BRE, a death receptor-associating intracellular protein, attenuates apoptotic response of human and mouse tumor cell lines to death receptor stimuli in vitro. In this report, we addressed whether the in vitro antiapoptotic effect of BRE could impact on tumor growth in vivo. We have shown that the mouse Lewis lung carcinoma D122 stable transfectants of human BRE expression vector developed into local tumor significantly faster than the stable transfectants of empty vector and parental D122, in both the syngeneic C57BL/6 host and nude mice. In vitro growth of the BRE stable transfectants was, however, not accelerated. No significant difference in metastasis between the transfectants and the parental D122 was detected. Thus, overexpression of BRE promotes local tumor growth but not metastasis. We conclude that the enhanced tumor growth is more likely due to the antiapoptotic activity of BRE than any direct effect of the protein on cell proliferation.
authors.affiliation: Clinical Immunology Unit and Sir Y.K. Pao Centre for Cancer, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China.
authors.forename: Ben Chung-Lap
authors.initials: BC
authors.lastname: Chan
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Stephanie Ka-Yee
authors.initials: SK
authors.lastname: Chow
authors.forename: Arthur Kar-Keung
authors.initials: AK
authors.lastname: Ching
authors.forename: Choong Tsek
authors.initials: CT
authors.lastname: Liew
authors.forename: Pak-Leong
authors.initials: PL
authors.lastname: Lim
authors.forename: Kenneth Ka-Ho
authors.initials: KK
authors.lastname: Lee
authors.forename: John Yeuk-Hon
authors.initials: JY
authors.lastname: Chan
authors.forename: Yiu-Loon
authors.initials: YL
authors.lastname: Chui
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C091514:BABAM2 protein, human; D009419:Nerve Tissue Proteins