title: Experimental generation of a tissue-engineered functional and vascularized trachea.
abstract: OBJECTIVE
  We sought to grow in vitro functional smooth muscle cells, chondrocytes, and respiratory epithelium on a biologic, directly vascularized matrix as a scaffold for tracheal tissue engineering.
  
  
  METHODS
  Ten- to 15-cm-long free jejunal segments with their own vascular pedicle were harvested and acellularized from donor pigs (n = 10) and used as a vascular matrix. Autologous costal chondrocytes, smooth muscle cells, and respiratory epithelium and endothelial progenitor cells were first cultured in vitro and then disseminated on the previously acellularized vascular matrix. Histologic, immunohistologic, molecular imaging, and Western blotting studies were then performed to assess cell viability.
  
  
  RESULTS
  The endothelial progenitor cells re-endothelialized the matrix to such an extent that endothelial cell viability was uniformly documented through 2-(18F)-fluoro-2'-deoxyglucose positron emission tomography. This vascularized scaffold was seeded with functional (according to Western blot analysis) smooth muscle cells and successfully reseeded with viable ciliated respiratory epithelium. Chondrocyte growth and production of extracellular cartilaginous matrix was observed as soon as 2 weeks after their culture.
  
  
  CONCLUSIONS
  The fundamental elements for a bioartificial trachea were successfully engineered in vitro in a direct vascularized 10- to 15-cm-long bioartificial matrix. Future experimental work will be directed to give them a 3-dimensional aspect and a biomechanical profile of a functioning trachea.
authors.affiliation: General Thoracic Surgery Biological Laboratory, Heidehaus Hospital, Hannover Medical School, Am Leineufer 70, D-30419 Hannover, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Walles
authors.forename: Bettina
authors.initials: B
authors.lastname: Giere
authors.forename: Michael
authors.initials: M
authors.lastname: Hofmann
authors.forename: Johanna
authors.initials: J
authors.lastname: Schanz
authors.forename: Fred
authors.initials: F
authors.lastname: Hofmann
authors.forename: Heike
authors.initials: H
authors.lastname: Mertsching
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchiarini
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D002478:Cells, Cultured; D007150:Immunohistochemistry; D032389:Myocytes, Smooth Muscle / Q000502:physiology; D013234:Stem Cells / Q000378:metabolism; D013552:Swine; D023822:Tissue Engineering; D014132:Trachea