Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case.
Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.
Dreesen
Laurent
L
Laboratoire de Spectroscopie Moléculaire de Surface, University of Namur, rue de Bruxelles, 61, 5000 Namur, Belgium. laurent.dreesen@fundp.ac.be
Sartenaer
Yannick
Y
Humbert
Christophe
C
Mani
Alaa A
AA
Méthivier
Christophe
C
Pradier
Claire-Marie
CM
Thiry
Paul A
PA
Peremans
André
A
D001360:Avidin / Q000737:chemistry*; D001665:Binding Sites; D002124:Calcium Fluoride / Q000737:chemistry; D018360:Crystallography, X-Ray; D008024:Ligands; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding / Q000502:physiology; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
D008024:Ligands; D001360:Avidin; C013411:biocytin; D008239:Lysine; D002124:Calcium Fluoride