title: Artocarpin is a polyspecific jacalin-related lectin with a monosaccharide preference for mannose.
abstract: A reinvestigation of the carbohydrate-binding properties revealed that artocarpin, a previously described mannose-specific lectin from jackfruit (Artocarpus integrifolia) seeds, behaves as a polyspecific lectin. Surface plasmon resonance hapten inhibition experiments demonstrated that artocarpin readily interacted with a wide range of monosaccharides covering galactose, N-acetylgalactosamine, mannose, glucose, sialic acid and N-acetylmuramic acid. Molecular docking confirmed this unexpected ability of artocarpin to interact with structurally different sugars. The biological significance of the polyspecificity of the lectin is discussed in terms of the broadening of the range of potential target glycans present on the surface of plant phytopathogens or predators.
authors.affiliation: Surfaces Cellulaires et Signalisation chez les Végétaux, UMR-CNRS 5546, Pôle de Biotechnologie Végétale, 24 chemin de Borde-Rouge, BP 17, Auzeville, 31326 Castanet-Tolosan, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Barre
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Peumans
authors.forename: Michel
authors.initials: M
authors.lastname: Rossignol
authors.forename: Gisèle
authors.initials: G
authors.lastname: Borderies
authors.forename: Raphaël
authors.initials: R
authors.lastname: Culerrier
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: Pierre
authors.initials: P
authors.lastname: Rougé
mesh_terms: D031622:Artocarpus / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D037241:Mannose-Binding Lectins / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C090683:artocarpin lectin; C043696:jacalin; D008358:Mannose