Crystallization and preliminary X-ray diffraction analysis of the unliganded human growth hormone receptor.
The crystal structure of the extracellular domain of growth hormone receptor complexed to its ligand, growth hormone, has been known since 1992. However, no information exists for the unliganded form of the receptor. The human growth hormone receptor's extracellular ligand-binding domain, encompassing amino-acid residues 1-238, has been expressed in Escherichia coli, purified by anion ion-exchange chromatography and crystallized in its unliganded state by the hanging-drop vapour-diffusion method in 100 mM HEPES pH 7.0 containing 27.5%(w/v) PEG 5000 monomethyl ether and 200 mM ammonium sulfate as the co-precipitants. The crystals belong to the othorhombic space group C222(1), have unit-cell parameters a = 99.7, b = 112.2, c = 93.2 A and diffract to 2.5 A resolution using synchrotron radiation. The crystal structure will shed light on the nature of any conformation changes that occur upon ligand binding and will provide information to develop potential low-molecular-weight agonists/antagonists to treat clinical diseases in which the growth hormone receptor is implicated.
McKinstry
William J
WJ
Biota Structural Biology Laboratory, St Vincent's Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria 3065, Australia. wmckinstry@svi.edu.au
Wan
Yu
Y
Adams
Julian J
JJ
Brown
Richard J
RJ
Waters
Michael J
MJ
Parker
Michael W
MW
D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003062:Codon; D003460:Crystallization; D018360:Crystallography, X-Ray; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
D003062:Codon; D008024:Ligands; D008565:Membrane Proteins; C405010:delta-hGHR