Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test.
A novel three-dimensional cell culture system was constructed with an array of cell panels (4 x 5) in a silicon chip, together with multi-channel drug containers. Human breast cancer (MCF-7) cells were embedded in a collagen-gel matrix and entrapped in a pyramidal-shaped silicon hole. Each cell panel can be isolated by a channel composed of a microfluid part and a reservoir. A cell panel was exposed to 200 mm KCN for 2 days to demonstrate that each cell panel could be independently evaluated under various stimulation conditions. Based on the cellular respiration activity, the proliferation behavior was continuously monitored on the silicon-based cell array for 5 days using scanning electrochemical microscopy (SECM). The cells entrapped in the device (3-D culture) proliferated normally, and the proliferation rate was lower than that of cells grown in a monolayer cell culture (2-D culture). The effects of three anticancer drugs measured simultaneously on the cell chip were in good agreement with those obtained by a conventional colorimetric assay. Our results suggest that the silicon-based device for 3D culture is appropriate for a chemosensitivity assay involving multi-chemical stimulation.
Torisawa
Y-S Yu-Suke
YS
Graduate School of Environmental Studies, Tohoku University, Aramaki Aoba 07, Sendai 980-8579, Miyagi, Japan.
Shiku
Hitoshi
H
Yasukawa
Tomoyuki
T
Nishizawa
Matsuhiko
M
Matsue
Tomokazu
T
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001943:Breast Neoplasms / Q000188:drug therapy*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D020527:Microscopy, Scanning Probe / Q000295:instrumentation* / Q000379:methods; D012825:Silicon / Q000737:chemistry
D000970:Antineoplastic Agents; D012825:Silicon