Effect of cyclodextrin on the activity and secondary structure of horseradish peroxidase.
The activity and secondary structure of horseradish peroxidase (HRP) was studied in aqueous solution containing alpha-, beta- and gamma-cyclodextrin (CD). The results showed that the activity of HRP was enhanced to different extents by the three kinds of CD. A Fourier Transform infrared (FTIR) spectroscopy study indicated that the amount of alpha-helical structure was important for the activity of HRP. This phenomenon is discussed.
Chen
Jian Bo
JB
College of Life and Environment Science, Shanghai Teachers University, Shanghai 200234, P.R. China.
Xu
Yi
Y
Xia
Chun Gu
CG
Li
Shu Ben
SB
D002942:Circular Dichroism; D003505:Cyclodextrins / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
D003505:Cyclodextrins; D006735:Horseradish Peroxidase; D010544:Peroxidases