title: Supercritical fluid extraction of sapogenins from tubers of Smilax china.
abstract: Supercritical CO(2) fluid extraction (SFE-CO(2)) was used to extract the sapogenins after acid hydrolysis from Smilax china tubers. The influence of extraction variables, such as modifier, pressure, temperature and extraction time, were studied. SFE-CO(2) was found to produce higher yield than conventional solvent extraction. The highest yield (0.454%) of sapogenins, mostly containing diosgenin, was obtained using 35 MPa pressure, 65 degrees C and 95% EtOH as a modifier for 180 min, higher than that obtained with conventional extraction methods (0.385%).
authors.affiliation: Institute of Materia Medica, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Xiao-shun
authors.initials: XS
authors.lastname: Shu
authors.forename: Zhong-hong
authors.initials: ZH
authors.lastname: Gao
authors.forename: Xiang-liang
authors.initials: XL
authors.lastname: Yang
mesh_terms: D025924:Chromatography, Supercritical Fluid; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D012502:Sapogenins / Q000737:chemistry*; D032281:Smilax
chemical_list: D010936:Plant Extracts; D012502:Sapogenins