Blue light delays commitment to cell division in Chlamydomonas reinhardtii.
In this study, we describe the effect of red and blue light on the timing of commitment to cell division in Chlamydomonas reinhardtii. The time point and cell size after which cells can complete their cell cycle with one division round were determined for cultures that were exposed to various red and blue light periods. We show that the commitment point of cells grown in blue light is shifted to a later time point and a larger cell size, when compared with cells grown in red light. This shift was reduced when cultures were exposed to shorter blue light periods. Furthermore, this shift occurred only when exposure to blue light started before the cells attained a particular size. We conclude that the critical cell size for cell division, which is the cell size at which commitment to cell division is attained, is dependent on spectral composition.
Oldenhof
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Laboratory of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands. jet_oldenhof@yahoo.com
Zachleder
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van den Ende
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D000818:Animals; D002455:Cell Division / Q000528:radiation effects*; D048429:Cell Size / Q000528:radiation effects; D002478:Cells, Cultured; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000528:radiation effects*; D008027:Light; D010316:Particle Size; D013997:Time Factors