title: The response of healing corneal epithelium to grooved polymer surfaces.
abstract: Corneal epithelial wounds heal rapidly by the inwards growth of tissue with a contracting wound front. A synthetic polymer lens to correct refractive error (an implantable contact lens) could be incorporated into the cornea using this wound healing process. Topographical cues on the polymer surface may facilitate epithelial tissue migration over the anterior device surface. Here, silicone discs with a defined surface geometry of parallel grooves (groove and ridge widths of 1, 2, 5 and 10 microm; groove depths of 1 and 5 microm) were implanted into corneas and maintained in organ culture. The nature and rate of epithelial tissue migration over the test surfaces was monitored for 8 days and evaluated using microscopy and histology. Irrespective of the pitch, deep groove geometries directed tissue migration laterally along the grooves but this prevented contraction of the wound front and retarded migration rates. No guidance occurred on any of the shallow groove geometries but these allowed inwards radial migration with a contracting wound front and supported migration rates equivalent to a flat surface. None of the geometries tested promoted tissue migration above a flat polymer surface and data suggested that parallel grooves may not be optimal for this application.
authors.affiliation: CSIRO Molecular Science, Riverside Corporate Park, 11 Julius Avenue, North Ryde, Sydney, NSW 1670, Australia. meg.evans@csiro.au
authors.forename: M D M
authors.initials: MD
authors.lastname: Evans
authors.forename: G A
authors.initials: GA
authors.lastname: McFarland
authors.forename: S
authors.initials: S
authors.lastname: Taylor
authors.forename: X F
authors.initials: XF
authors.lastname: Walboomers
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion; D002465:Cell Movement; D004728:Endothelium, Corneal / Q000293:injuries / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D020007:Implants, Experimental; D008422:Materials Testing; D009924:Organ Culture Techniques; D012828:Silicones / Q000737:chemistry*; D013499:Surface Properties; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D012828:Silicones