title: [Detection of bacteria contaminating blood for transfusion].
abstract: When Escherichia coli is cultured in nutrient broth at 1 x 10(3) cells/ml, 7.5 h are needed to detect cell growth as turbidity. The time to detect E. coli in this medium is reduced using the alamar blue method. Alamar blue is an oxidation-reduction indicator that changes color in response to cell growth. E. coli can grow in nutrient broth containing red blood cells, but the detection of E. coli based on turbidity is difficult because the red blood cells muddy the medium. As the change in color in the alamar blue method is not affected by red blood cells, the growth of E. coli contaminating red blood cells is detectable. These results suggest that a cell growth indicator such as alamar blue is useful for the detection of bacteria contaminating blood for transfusion.
authors.affiliation: Department of Microbiology, Tohoku Pharmaceutical University, Sendai 981-8558, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Osanai
authors.forename: Miho
authors.initials: M
authors.lastname: Saito
authors.forename: Namie
authors.initials: N
authors.lastname: Tamura
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Watanabe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mikami
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004340:Drug Contamination; D004912:Erythrocytes / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification*; D006801:Humans; D007202:Indicators and Reagents; D010078:Oxazines; D065227:Transfusion Reaction; D014966:Xanthenes
chemical_list: D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin