Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C epsilon*).
We have isolated a novel cDNA from the human fetal brain cDNA library with homology to the Mg2+ -dependent serine/threonine protein phosphatase 2C (PP2C) family. The cDNA is 3055 bp in length, and the predicted coding region encodes a 360-amino-acid protein, which shows 99% identity to the PP2C epsilon from rat and mouse. Then we term it human PP2C epsilon gene. The gene is mapped to chromosome 3q26.1 and contains 4 exons. RT-PCR analysis shows that the PP2C epsilon is widely expressed in human tissues and the expression levels in heart, placenta, lung, liver, kidney, and pancreas are relatively high.
Jin
Feng
F
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Ji
Chaoneng
C
Liu
Lingfeng
L
Dai
Jianfeng
J
Gu
Shaohua
S
Sun
Xianfei
X
Xie
Yi
Y
Mao
Yumin
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D015723:Gene Library; D006801:Humans; D007438:Introns; D020935:MAP Kinase Signaling System; D008274:Magnesium / Q000737:chemistry; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D000071636:Protein Phosphatase 2C; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; C000607456:PPM1A protein, human; C000607457:PPM1B protein, human; C000607460:PPM1G protein, human; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; D008274:Magnesium