Electrophoresis microchip fabricated by a direct-printing process with end-channel amperometric detection.
We describe the development of an electrophoresis microchip fabricated by a direct-printing process, based on lamination of printed polyester films with end-channel amperometric detection. The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester-toner devices presented an electroosmotic flow (EOF) magnitude of approximately 10(-5) cm2 V(-1) s(-1), which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser-printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol.L(-1) (135 amol) and 1.8 micromol.L(-1) (486 amol) for iodide and ascorbate, respectively.
Coltro
Wendell K Tomazelli
WK
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos-SP, Brazil.
da Silva
José A Fracassi
JA
da Silva
Heron D Torres
HD
Richter
Eduardo M
EM
Furlan
Rogério
R
Angnes
Lúcio
L
do Lago
Claudimir L
CL
Mazo
Luiz H
LH
Carrilho
Emanuel
E
D001205:Ascorbic Acid / Q000032:analysis; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D007454:Iodides / Q000032:analysis; D008420:Manufactured Materials; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011091:Polyesters
D007454:Iodides; D011091:Polyesters; D001205:Ascorbic Acid