Trichinella spiralis: stimulation of mast cells by TSL-1 antigens trigger cytokine mRNA expression and release of IL-4 and TNF through an Ig-independent pathway.
Previous in vitro studies have demonstrated that mast cells (MC) can be directly activated by Trichinella spiralis larvae 1 (TSL-1) antigens. To characterize even more this activation of MC and their possible role on induction and regulation of the Type 2 response generated against T. spiralis infection, we studied the interaction between a hybrid rat MC line (HRMC), murine bone marrow MC (mBMMC), and TSL-1 antigens. Immunofluorescence staining and flow cytometry analysis showed that TSL-1 antigens bound to the surface of HRMC cells, resulting in the transcriptional induction and in the release of TNF and IL-4. Besides, an increase of IL-4 intracellular expression was also observed in mBMMC. This suggests that MC may play an important role in the early immune response against T. spiralis and may be a source of cytokines, that regulate the final onset of the immune mechanisms which determine the course of the infection.
Niborski
Violeta
V
UMR BIPAR INRA-AFSSA-ENVA-UPVM, Maisons-Alfort, France.
Vallée
Isabelle
I
Fonseca-Liñán
Rocío
R
Boireau
Pascal
P
Enciso
Antonio
A
Ortega-Pierres
Guadalupe
G
Yépez-Mulia
Lilián
L
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000276:immunology; D008407:Mast Cells / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017160:Trichinella spiralis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D000947:Antigens, Helminth; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4