title: The G/C915 polymorphism of transforming growth factor beta1 is associated with human longevity: a study in Italian centenarians.
abstract: Sequence variations in a variety of pro- or anti-inflammatory cytokine genes have been found to influence successful aging and longevity. Because of the role played by the transforming growth factor beta1 (TGF-beta1) cytokine in inflammation and regulation of immune responses, the variability of the TGF-beta1 gene may affect longevity by playing a role in inflamm-aging. Two polymorphisms, G/A -800 and C/T -509, located in the 5' region, and two missense polymorphisms, T/C 869 and G/C 915 which change (Leu > Pro)10 and (Arg > Pro)25, respectively, located in the signal peptide, were analysed in 419 subjects from Northern and Central Italy, including 172 centenarians and 247 younger controls. In addition, the effects of the TGF-beta1 genetic variability on plasma levels of the biologically active form (naturally processed) of this cytokine were studied in 143 randomly selected subjects, including 73 centenarians. Significant differences were found at the +915 site as far as the C allele and GC genotype were concerned, both of them being lower in centenarians than in young controls (P=0.034 and 0.028, respectively), but none of the other tested genetic variants was significantly different between centenarians and controls. Moreover, a particular haplotype combination (G -800/C -509/C 869/C 915) was notably lower in centenarians than in younger individuals (P=0.007). Finally, active TGF-beta1 plasma levels were significantly increased in the elderly group, but no relationship with TGF-beta1 genotypes was observed. These results suggest that, at least in this population, the variability of the TGF-beta1 gene influences longevity and that the age-related increase in plasma levels of active TGF-beta1 seems not to be genetically regulated.
authors.affiliation: I.N.R.C.A., Italian National Research Center on Aging, Ancona, Italy. b.molecolare@inrca.it
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Carrieri
authors.forename: Erika
authors.initials: E
authors.lastname: Marzi
authors.forename: Fabiola
authors.initials: F
authors.lastname: Olivieri
authors.forename: Francesca
authors.initials: F
authors.lastname: Marchegiani
authors.forename: Luca
authors.initials: L
authors.lastname: Cavallone
authors.forename: Maurizio
authors.initials: M
authors.lastname: Cardelli
authors.forename: Simona
authors.initials: S
authors.lastname: Giovagnetti
authors.forename: Rosalia
authors.initials: R
authors.lastname: Stecconi
authors.forename: Cinzia
authors.initials: C
authors.lastname: Molendini
authors.forename: Chiara
authors.initials: C
authors.lastname: Trapassi
authors.forename: Giovanna
authors.initials: G
authors.lastname: De Benedictis
authors.forename: Dimitri
authors.initials: D
authors.lastname: Kletsas
authors.forename: Claudio
authors.initials: C
authors.lastname: Franceschi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics / Q000276:immunology; D000483:Alleles; D016207:Cytokines / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007558:Italy; D008136:Longevity / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012727:Sex Characteristics; D016212:Transforming Growth Factor beta / Q000097:blood / Q000235:genetics*; D053773:Transforming Growth Factor beta1
chemical_list: D016207:Cytokines; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1