Isolation and characterization of neural stem cells from human fetal striatum.
This paper described that neural stem cells (hsNSCs) were isolated and expanded rapidly from human fetal striatum in adherent culture. The population was serum- and growth factor-dependent and expressed neural stem cell markers. They were capable of multi-differentiation into neurons, astrocytes, and oligodendrocytes. When plated in the dopaminergic neuron inducing medium, human striatum neural stem cells could differentiate into tyrosine hydroxylase positive neurons. hsNSCs were morphologically homogeneous and possessed high proliferation ability. The population doubled every 44.28h and until now it has divided for more than 82 generations in vitro. Normal human diploid karyotype was unchanged throughout the in vitro culture period. Together, this study has exploited a method for continuous and rapid expansion of human neural stem cells as pure population, which maintained the capacity to generate almost fifty percent neurons. The availability of such cells may hold great interest for basic and applied neuroscience.
Li
Xiaoxia
X
Department of Cell Biology, Peking University Health Science Center, Peking University Stem Cell Research Center, Beijing 100083, China.
Xu
Jinchong
J
Bai
Yun
Y
Wang
Xuan
X
Dai
Xin
X
Liu
Yinan
Y
Zhang
Jun
J
Zou
Junhua
J
Shen
Li
L
Li
Lingsong
L
D001253:Astrocytes / Q000166:cytology; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D005333:Fetus; D006801:Humans; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D017072:Neostriatum / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
D015415:Biomarkers; D012333:RNA, Messenger