[Application of PSD-MALDI-TOF-MS to protein identification].
OBJECTIVE
To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.


METHODS
The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.


RESULTS
Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.


CONCLUSIONS
PSD technique has greater application prospects in peoteomics.
Wang
Jie
J
National Center of Biomedical Analysis, Beijing 100850, China.
Yang
Song-cheng
SC
Wu
Sheng-ming
SM
Wang
Hong-xia
HX
Wei
Kai-hua
KH
Zhang
Xue-min
XM
D000324:Adrenocorticotropic Hormone / Q000737:chemistry*; D000595:Amino Acid Sequence; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D012269:Ribosomal Proteins / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D010959:Tissue Plasminogen Activator / Q000737:chemistry*
D010446:Peptide Fragments; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D000324:Adrenocorticotropic Hormone; D010959:Tissue Plasminogen Activator