The budding yeast mei5 and sae3 proteins act together with dmc1 during meiotic recombination.
Here we provide evidence that the Mei5 and Sae3 proteins of budding yeast act together with Dmc1, a meiosis-specific, RecA-like recombinase. The mei5 and sae3 mutations reduce sporulation, spore viability, and crossing over to the same extent as dmc1. In all three mutants, these defects are largely suppressed by overproduction of Rad51. In addition, mei5 and sae3, like dmc1, suppress the cell-cycle arrest phenotype of the hop2 mutant. The Mei5, Sae3, and Dmc1 proteins colocalize to foci on meiotic chromosomes, and their localization is mutually dependent. The localization of Rad51 to chromosomes is not affected in either mei5 or sae3. Taken together, these observations suggest that the Mei5 and Sae3 proteins are accessory factors specific to Dmc1. We speculate that Mei5 and Sae3 are necessary for efficient formation of Dmc1-containing nucleoprotein filaments in vivo.
Tsubouchi
Hideo
H
Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06520-8103, USA.
Roeder
G Shirleen
GS
D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003434:Crossing Over, Genetic; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004706:Endodeoxyribonucleases; D004950:Esterases / Q000378:metabolism; D008540:Meiosis / Q000502:physiology*; D008969:Molecular Sequence Data; D045522:Recombinases; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D004718:Saccharomycetales / Q000235:genetics / Q000502:physiology*; D000099257:Meiotic Recombination Protein SPO11
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D004950:Esterases; D045522:Recombinases; D029701:Saccharomyces cerevisiae Proteins; D000099257:Meiotic Recombination Protein SPO11; C513033:DMC1 protein, S cerevisiae; C495980:Mei5 protein, S cerevisiae; C107851:SAE3 protein, S cerevisiae