title: [Studies on the transfection of umbilical endothelia with catalytic subunit of telomerase].
abstract: OBJECTIVE
  To investigate the variety of proliferating ability of umbilical endothelia (UE) transfected by plasmid pBABE-HYGR-hTERT.
  
  
  METHODS
  UE was identified from two aspects: morphology and CD34 labeling technique. The plasmid was obtained and identified by alkali splitting and gel electrophoresis. Liposomes were used to transfect UE. RT-PCR based telomeric repeat amplification protocol (TRAP) assay was used to measure the telomerase activity of endothelia.
  
  
  RESULTS
  UE arranged as "cobblestone" and were positive of CD34 labeling. Endothelia transfected by pBABE-HYGR-hTERT(HC) had an raised absorbance of 0.889. The shape of growth curve of HC was similar to UE. But the absorbance of MTT test and the amount of HC were prior to UE at every measuring time and the amount of HC increased four times within 8 days (P < 0.05).
  
  
  CONCLUSIONS
  The transfection of pBABE-HYGRO-hTERT had greatly improved the proliferating abilities and activated the telomerase of UE.
authors.affiliation: Key Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Dai
authors.forename: Long-jiang
authors.initials: LJ
authors.lastname: Li
authors.forename: Yu-ming
authors.initials: YM
authors.lastname: Wen
authors.forename: Chang-mei
authors.initials: CM
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Sheng-fu
authors.initials: SF
authors.lastname: Li
mesh_terms: D020134:Catalytic Domain / Q000235:genetics*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D019098:Telomerase / Q000235:genetics*; D014162:Transfection; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: D019098:Telomerase