title: [Studies on sex identification and variation of endogenous hormones in female and male plants of Gynostemma pentaphyllum].
abstract: OBJECTIVE
  To distinguish female and male plants of Gynostemma pentaphyllum quickly and accurately in the stage of seedling and to understand the relation between sex differentiation and endogenous plant hormone.
  
  
  METHODS
  Stained methods of BTB and enzyme linked immuno-sorbent Assay were used.
  
  
  RESULTS
  10 Hours after being dyed, the liquid extract of female plants turned into yellow, while extract of male ones turned green. There were obvious differences on iPAs and GA(1 + 3) contents but no differences on IAA and ABA contents between male and female plants during the stages of seedling and vegetative growth.
  
  
  CONCLUSIONS
  BTB can be used for distinguishing female and male plants and there were a certain relation between sex and endogenous plant hormones.
authors.affiliation: College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. qingyawang@yahoo.com.cn
authors.forename: Qing-ya
authors.initials: QY
authors.lastname: Wang
authors.forename: Qiao-sheng
authors.initials: QS
authors.lastname: Guo
authors.forename: Jian-yun
authors.initials: JY
authors.lastname: Sun
authors.forename: Shou-dong
authors.initials: SD
authors.lastname: Zhang
authors.forename: Yang-han
authors.initials: YH
authors.lastname: Li
mesh_terms: D005875:Gibberellins / Q000378:metabolism*; D029774:Gynostemma / Q000254:growth & development / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism*; D012737:Sex Factors
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators