Antifungal activity of amphotericin B and itraconazole against filamentous fungi: comparison of the Sensititre Yeast One and NCCLS M38--a reference methods.
The susceptibilities of 81 clinical isolates of Aspergillus spp., Fusarium spp., and Scedosporium spp., to amphotericin B and itraconazole were determined by the colorimetric microdilution method Sensititre and the reference microdilution method of NCCLS standard M38-A for filamentous fungi. No major discrepancies were found and agreement ranged between 86.4% to 84% and 69.1% to 86.4% for amphotericin B and itraconazole respectively at 48 h and 72 h of incubation by using the recommended endpoints. Within two two-fold dilutions, high levels of agreement were found in general for amphotericin B at 48 or 72 h (86.4 to 87.7%) and itraconazole (91.4 to 93.8%). Relatively better agreement was found for itraconazole at 72 h of incubation and 48 for amphotericin B.
Carrillo-Muñoz
A J
AJ
ACIA, Barcelona, Spain acarrillo@ya.com
Quindós
G
G
Del Valle
O
O
Hernández-Molina
J M
JM
Santos
P
P
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D003904:Mitosporic Fungi / Q000187:drug effects*; D011237:Predictive Value of Tests; D021681:Scedosporium / Q000187:drug effects; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D017964:Itraconazole; D000666:Amphotericin B