[Regulation of Fc receptor expression by immune complexes on neutrophils and U937 cells].
OBJECTIVE
To study the regulation of Fc receptor expression by immune complexes (ICs) on neutrophils and U937 cells.


METHODS
IgA ICs, IgG1 ICs, IgG2 ICs, IgG3 ICs, IgG4 ICs, and IgM ICs were incubated with neutrophils or U937 cells for 1 h. Then their surface Fc receptors were stained by anti-Fc gammaR I, anti-Fc gammaR II , anti-Fc gammaR III, and anti-Fc alphaR I monoclonal antibodies and analyzed by fluorescent activated cell sorting (FACS).


RESULTS
IgG1 ICs and IgG3 ICs up-regulated Fc gammaR II and Fc gammaR III on U937 cells, Fc gammaR I and Fc alphaR I on neutrophils. Almost all ICs down-regulated Fc gammaR II on neutrophils.


CONCLUSIONS
ICs can regulate Fc receptor expression on neutrophils and U937 cells, among which IgG1 ICs and IgG3 ICs are most effective.
Guo
Li-hua
LH
Department of Immunology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Zhang
Wei
W
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000936:Antigen-Antibody Complex / Q000276:immunology* / Q000378:metabolism; D015703:Antigens, CD / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000145:classification / Q000276:immunology; D007074:Immunoglobulin G / Q000737:chemistry / Q000145:classification / Q000276:immunology / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D011961:Receptors, Fc / Q000096:biosynthesis* / Q000235:genetics; D017452:Receptors, IgG / Q000276:immunology*; D020298:U937 Cells / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D015703:Antigens, CD; C080568:Fc gamma receptor IIA; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; C048844:anti-IgG