Expression of thrombospondin-1 in prostate-derived cell lines.
Thrombospondin-1 (TSP-1) is a multifunctional matricellular glycoprotein involved in several mechanisms critical to the formation and progression of solid tumors including cell adhesion, proliferation, migration, invasion, and angiogenesis. However, work related to TSP-1 expression and functionality in prostate cancer is limited. Expression experiments in the present study demonstrated lower expression of TSP-1 in the prostate cancer cell lines DU 145 and LNCaP compared to SV40-immortalized prostatic epithelial cells PNT 1A. All three cell lines expressed the TSP-1 receptor CD36. Exogenously added TSP-1 modulated the cellular phenotype of LNCaP cells, which demonstrated decreased proliferation rate and partly entered apoptosis. Collectively, these data support the concept that partial or complete loss of TSP-1 synthesis may provide tumor cells with a proliferation advantage. In addition, TSP-1 located at the border between tumor and stroma as observed in primary prostate tumors may act as a barrier of tumor growth depending on the TSP-1 receptor repertoire of the tumor cells.
Bastian
Manuela
M
University of Rostock, Institute of Clinical Chemistry and Pathobiochemistry, D-18057 Rostock, Germany. manuela.bastian@med.uni-rostock.de
Steiner
Michael
M
Schuff-Werner
Peter
P
D000135:Acid Phosphatase / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D018955:CD36 Antigens / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission; D011467:Prostate / Q000166:cytology* / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D013917:Thrombin / Q000494:pharmacology; D019700:Thrombospondin 1 / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology
D018955:CD36 Antigens; D019700:Thrombospondin 1; D000135:Acid Phosphatase; D013917:Thrombin