title: Direct proteins electrochemistry based on ionic liquid mediated carbon nanotube modified glassy carbon electrode.
abstract: A novel glassy carbon electrode modified by a gel containing multi-walled carbon nanotubes (MWNTs) and ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIPF6) is reported. The gel is formed by grinding of MWNTs and BMIPF6. Such gel is then coated on the surface of a glassy carbon electrode. We have employed scanning electron microscopy, Fourier transform infrared spectrometry (FTIR) and cyclic voltammetry to characterize the modified electrode. The direct electron transfers of hemoglobin and catalase on the modified electrode have been observed and studied in detail electrochemically. Hemoglobin is verified to be adsorbed on the modified electrode with the retention of conformation, which has been proved by microscopic FTIR. The electrochemical response of the adsorbed hemoglobin on the modified electrode is very stable, and shows repeated changes in the different pH solutions. It also has shown electrocatalysis to the reduction of oxygen and trichloroacetic acid. Catalase adsorbed on the gel modified electrode still keep activity to hydrogen peroxide. This work provides a simple and easy approach to construct biosensors based on the carbon nanotubes and ionic liquids.
authors.affiliation: College of Chemistry and Molecular Engineering, Peking University, Beijing, China 100871.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Dongping
authors.initials: D
authors.lastname: Zhan
authors.forename: Hongyang
authors.initials: H
authors.lastname: Ma
authors.forename: Meiqin
authors.initials: M
authors.lastname: Zhang
authors.forename: Yifang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Jing
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xinhua
authors.initials: X
authors.lastname: Wan
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Shao
authors.forename: Qiankun
authors.initials: Q
authors.lastname: Zhuang
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002374:Catalase / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004583:Electrons; D006454:Hemoglobins / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D007477:Ions; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014238:Trichloroacetic Acid / Q000737:chemistry
chemical_list: C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D006454:Hemoglobins; D007093:Imidazoles; D007477:Ions; D037742:Nanotubes, Carbon; D014238:Trichloroacetic Acid; D002244:Carbon; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen