Isolation of angiotensin converting enzyme (ACE) inhibitory flavonoids from Sedum sarmentosum.
Bioassay-guided fractionation of the EtOAc-soluble extract of Sedum sarmentosum afforded a new flavonoid, quercetin-3-O-alpha-(6'''-caffeoylglucosyl-beta-1,2-rhamnoside) (1), along with four known flavonoids, quercetin 3-O-alpha-(6'''-p-coumaroylglucosyl-beta-1,2-rhamnoside) (2), isorhamnetin-3-beta-glucopyranoside (3), quercetin-3-beta-glucopyranoside (4), and kaempferol-3-alpha-arabinopyranoside (5). Purification of these compounds was conducted with the application of various chromatographic methods. Compounds 1-5 inhibited angiotensin I converting enzyme (ACE) activity in a concentration-dependent manner. Compounds 1-5 had 50% inhibitory concentration values of 158.9+/-11.1 microgM, 351.6+/-3.9 microgM, 408.9+/-4.6 microgM, 708.8+/-23.1 microgM, and 392.8+/-13.4 microgM.
Oh
Hyuncheol
H
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Kang
Dae-Gill
DG
Kwon
Ji-Woong
JW
Kwon
Tae-Oh
TO
Lee
Seung-Yeob
SY
Lee
Duck-Bae
DB
Lee
Ho-Sub
HS
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D005419:Flavonoids / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D031963:Sedum
D000806:Angiotensin-Converting Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; D007703:Peptidyl-Dipeptidase A