title: Mass spectrometric and high performance liquid chromatography profiling of the venom of the Brazilian vermivorous mollusk Conus regius: feeding behavior and identification of one novel conotoxin.
abstract: Carnivorous mollusks belonging to the genus Conus paralyze their prey by injecting a rich mixture of biologically active peptides. Conus regius is a vermivorous member of this genus that inhabits Brazilian tropical waters. Inter-, intra-species and individual variations of cone snail venom have been previously reported. In order to investigate intra-specific differences in C. regius venom, its feeding behavior and the correlation between these two factors, animals were pooled according to gender, size and season of collection, and their venom composition was compared by high performance liquid chromatography (HPLC). Both the whole venom and one specific peak were monitored by HPLC. Chromatographic profiles revealed no significant differences in their peak areas, indicating that the venom composition, based solely in the presence or absence of the major peaks, is stable regardless of season, gender and size. Therefore, analysis of one given toxin, eluting in one of the major peaks, is representative among the population. Moreover, this work presents the identification of one novel conotoxin (rg11a), which amino acid sequence was deduced by mass spectrometry.
authors.affiliation: Department of Physiology, Bioscience Institute, University of São Paulo, São Paulo, SP, Brazil. mcbraga@usp.br
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Vianna Braga
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Konno
authors.forename: Fernanda C V
authors.initials: FC
authors.lastname: Portaro
authors.forename: José Carlos
authors.initials: JC
authors.lastname: de Freitas
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yamane
authors.forename: Baldomero M
authors.initials: BM
authors.lastname: Olivera
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Pimenta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049628:Body Size; D002851:Chromatography, High Pressure Liquid; D020916:Conotoxins / Q000737:chemistry / Q000302:isolation & purification*; D005247:Feeding Behavior / Q000502:physiology*; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D012908:Snails / Q000737:chemistry* / Q000502:physiology
chemical_list: D020916:Conotoxins