title: High-resolution NMR spectroscopy of membrane proteins in aligned bicelles.
abstract: High-resolution solid-state NMR spectra can be obtained from uniformly (15)N-labeled membrane proteins in magnetically aligned bicelles. Fast uniaxial diffusion about the axis of the bilayer normal results in single-line spectra that contain the orientational information necessary for protein structure determination.
authors.affiliation: Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307, USA.
authors.forename: Anna A
authors.initials: AA
authors.lastname: De Angelis
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Nevzorov
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Stanley C
authors.initials: SC
authors.lastname: Howell
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Mrse
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D010767:Phosphorylcholine