Purification of the isoflavonoid puerarin by adsorption chromatography on cross-linked 12% agarose.
The isoflavonoid puerarin in extracts of the well-known traditional Chinese drug Radix puerariae (root of the plant Pueraria lobata) can be separated from other isoflavonoids by adsorption chromatography on the cross-linked 12% agarose gel Superose 12 equilibrated in distilled water. The adsorption is totally quenched by the addition of 50% acetic acid. The separation of the isoflavonoids is tentatively ascribed to interaction with the residues of the cross-linking reagents used in the manufacturing process of Superose 12. Thus, no useful separation can be achieved with non-cross-linked 12% agarose gel media. Symmetric elution profiles at high sample loadings (16 mg on a 24 ml column) suggest linear adsorption isotherms for the isoflavonoids.
He
Xiangling
X
Department of Biotechnology, School of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Tan
Tianwei
T
Janson
Jan-Christer
JC
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007529:Isoflavones / Q000302:isolation & purification*; D012685:Sepharose / Q000737:chemistry*
D007529:Isoflavones; D012685:Sepharose; C033607:puerarin