In vitro selective suppression of feline myeloid colony formation is attributable to molecularly cloned strain of feline leukemia virus with unique long terminal repeat.
Molecularly cloned feline leukemia virus (FeLV)-clone 33 (C-33), derived from a cat with acute myelocytic leukemia (AML), was examined to assess its relation to the pathogenesis of AML and myelodysplastic syndrome (MDS). To evaluate in vitro pathogenicity of FeLV C-33, bone marrow colony-forming assay was performed on marrow cells infected with FeLV C-33 or an FeLV subgroup A strain (61E, a molecularly cloned strain with minimal pathogenicity). The myeloid colony-forming activity of feline bone marrow mononuclear cells infected with FeLV C-33 was significantly lower than that of cells infected with 61E. This suggests that FeLV C-33 has myeloid lineage-specific pathogenicity for cats, and that FeLV C-33 infection is useful as an experimental model for investigating pathogenesis of MDS and AML.
Nagashima
N
N
Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Hisasue
M
M
Nishigaki
K
K
Miyazawa
T
T
Kano
R
R
Hasegawa
A
A
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000821:virology; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005256:Leukemia Virus, Feline / Q000235:genetics* / Q000472:pathogenicity*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D009190:Myelodysplastic Syndromes / Q000662:veterinary / Q000821:virology; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012192:Retroviridae Infections / Q000662:veterinary* / Q000821:virology; D020079:Terminal Repeat Sequences; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral