Supercritical fluid extraction of sapogenins from tubers of Smilax china.
Supercritical CO(2) fluid extraction (SFE-CO(2)) was used to extract the sapogenins after acid hydrolysis from Smilax china tubers. The influence of extraction variables, such as modifier, pressure, temperature and extraction time, were studied. SFE-CO(2) was found to produce higher yield than conventional solvent extraction. The highest yield (0.454%) of sapogenins, mostly containing diosgenin, was obtained using 35 MPa pressure, 65 degrees C and 95% EtOH as a modifier for 180 min, higher than that obtained with conventional extraction methods (0.385%).
Shu
Xiao-shun
XS
Institute of Materia Medica, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Gao
Zhong-hong
ZH
Yang
Xiang-liang
XL
D025924:Chromatography, Supercritical Fluid; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D012502:Sapogenins / Q000737:chemistry*; D032281:Smilax
D010936:Plant Extracts; D012502:Sapogenins