Chemiluminescent analysis of the antioxidant and immunomodulation effects of several psychotropic drugs on peritoneal macrophages.
The present study describes the application of several chemiluminescent (CL) methods for evaluation of antioxidant and immunomodulation effects of psychotropic drugs upon phagocytes: KO2-induced luminal-dependent CL for detection of superoxide anion radicals in a pure chemical system; PMA- and A23187-induced CL of peritoneal macrophages for detection of free radicals in cell suspension; and CL, produced by the luciferase-catalyzed luciferin + ATP reaction, for evaluation of cell viability before and after drug application. These methods provide also a way to investigate the location of drug action. It was found that the psychotropic drugs in fluence the 'oxidative burst' of macrophages through two mechanisms: by expression of drug antioxidant properties and/or by a direct immunomodulation effect.
Hadjimitova
V
V
Department of Medical Physics and Biophysics, Medical University, 2 Zdrave Str., Sofia 1431, Bulgaria. verah@mail.bg
Traykov
T
T
Bakalova
R
R
Petrova
V
V
Lambev
I
I
Ohba
H
H
Ishikawa
M
M
Baba
Y
Y
D000639:Amitriptyline / Q000494:pharmacology; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D014150:Antipsychotic Agents / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology; D002749:Chlorprothixene / Q000494:pharmacology; D007099:Imipramine / Q000494:pharmacology; D007155:Immunologic Factors / Q000494:pharmacology*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007476:Ionophores / Q000494:pharmacology; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D011619:Psychotropic Drugs / Q000494:pharmacology*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013892:Thioxanthenes / Q000494:pharmacology
D000929:Antidepressive Agents, Tricyclic; D000975:Antioxidants; D014150:Antipsychotic Agents; D002147:Calmodulin; D007155:Immunologic Factors; D007202:Indicators and Reagents; D007476:Ionophores; D011619:Psychotropic Drugs; D013892:Thioxanthenes; D000639:Amitriptyline; D000001:Calcimycin; D008165:Luminol; D002749:Chlorprothixene; D013755:Tetradecanoylphorbol Acetate; D007099:Imipramine; D002746:Chlorpromazine