title: [The relationship between the intracellular redox status of immune cells and progression of hepatitis C virus related chronic liver disease].
abstract: It has been suggested that oxidative stress participates in the pathogenesis of hepatitis C virus infection. It also has been made clear that redox status in T cell and macrophage relates to the activity of virus infectious disease such as HIV infection. With such background we evaluated the relationship between the intracellular redox status of T cell and macrophage and the activity of HCV positive chronic liver disease. Intracellular GSH and GSSG levels of T cell and macrophage were determined in twenty-five HCV positive asymptomatic carriers (C-ASC), sixty-three chronic hepatitis patients (C-CH), ten HCV positive liver cirrhosis patients (C-LC) and twenty-nine healthy controls. The intracellular GSH levels of T cell (T-GSH) significantly decreased in both C-CH and C-LC compared with healthy controls. No significant differences in the T-GSH levels were found between healthy controls and C-ASC. T-GSH levels of C-CH and C-LC were significantly lower compared with C-ASC. The intracellular GSH levels of macrophage (CD14-GSH) of C-LC were significantly decreased compared with healthy controls. The CD14-GSH levels of C-CH and C-LC were significantly lower compared with C-ASC. There was no correlation between intracellular GSH, GSSG levels and the serum levels of iron-related markers, fibrogenesis markers and other clinical parameters. These results suggest that the intracellular redox status of T cell and macrophage relates to the progression of HCV related chronic liver disease.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Onoda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Masahisa
authors.initials: M
authors.lastname: Osada
authors.forename: Akira
authors.initials: A
authors.lastname: Saito
authors.forename: Mikio
authors.initials: M
authors.lastname: Zeniya
authors.forename: Gotaro
authors.initials: G
authors.lastname: Toda
mesh_terms: D000368:Aged; D018450:Disease Progression; D005260:Female; D005978:Glutathione / Q000097:blood; D019803:Glutathione Disulfide / Q000097:blood; D019698:Hepatitis C, Chronic / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
chemical_list: D005978:Glutathione; D019803:Glutathione Disulfide