Enhancing the first enzymatic step in the histidine biosynthesis pathway increases the free histidine pool and nickel tolerance in Arabidopsis thaliana.
Naturally selected nickel (Ni) tolerance in Alyssum lesbiacum has been proposed to involve constitutively high levels of endogenous free histidine. Transgenic Arabidopsis thaliana expressing a Salmonella typhimurium ATP phosphoribosyl transferase enzyme (StHisG) resistant to feedback inhibition by histidine contained approximately 2-fold higher histidine concentrations than wild type plants. Under exposure to a toxic Ni concentration, biomass production in StHisG expressing lines was between 14- and 40-fold higher than in wild-type plants. This suggested that enhancing the first step in the histidine biosynthesis pathway is sufficient to increase the endogenous free histidine pool and Ni tolerance in A. thaliana.
Wycisk
Katharina
K
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany.
Kim
Eugene J
EJ
Schroeder
Julian I
JI
Krämer
Ute
U
D001276:ATP Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D006639:Histidine / Q000096:biosynthesis*; D009532:Nickel / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified; D012989:Soil Pollutants
D001426:Bacterial Proteins; D012989:Soil Pollutants; D006639:Histidine; D009532:Nickel; D001276:ATP Phosphoribosyltransferase