Histidine supplementation suppresses food intake and fat accumulation in rats.
OBJECTIVE
Histamine, a derivative of histidine, decreases food intake and body fat by activation of histamine neurons. Our objective was to clarify the effect of dietary histidine, in particular, on food intake and/or body fat accumulation in rats.


METHODS
Male Wistar rats were assigned to one of four groups after acclimation and allowed free access to diets containing 20% casein (0% histidine), 20% casein plus 1.0% histidine, 20% casein plus 2.5% histidine, or 20% casein plus 5% histidine for 8 d.


RESULTS
Food intake and body weight were recorded daily and compared between groups. During the experimental period, food intake decreased according to the increases in dietary histidine. There was a negative and significant (P < 0.01) correlation between dietary histidine (grams per 8 d) and retroperitoneal fat pad (grams per 100 g of body weight). Uncoupling protein-1 mRNA in brown adipose tissue increased with increases in dietary histidine.


CONCLUSIONS
Our results indicate that dietary histidine suppresses food intake and fat accumulation in rats.
Kasaoka
Seiichi
S
Department of Health and Nutrition, Bunkyo University Women's College, Kanagawa, Japan. kasaoka@shonan.bunkyo.ac.jp
Tsuboyama-Kasaoka
Nobuyo
N
Kawahara
Yumi
Y
Inoue
Setsuko
S
Tsuji
Makiko
M
Ezaki
Osamu
O
Kato
Hideo
H
Tsuchiya
Takahide
T
Okuda
Hiromichi
H
Nakajima
Shigeru
S
D000273:Adipose Tissue / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000187:drug effects / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects*; D006639:Histidine / Q000008:administration & dosage*; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D024101:Mitochondrial Proteins; D009765:Obesity / Q000517:prevention & control; D012333:RNA, Messenger / Q000378:metabolism; D000077278:RNA, Mitochondrial; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D004044:Dietary Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; C029305:mitochondrial messenger RNA; D006639:Histidine