title: Down-regulation of human deoxyuridine triphosphate nucleotidohydrolase (dUTPase) using small interfering RNA (siRNA).
abstract: A small interfering double stranded RNA molecule (siRNA, 21 bp) corresponding to a portion (nucleotides 337 to 357) of domain 3 of the human dUTPase was synthesized and used to determine whether it could down-regulate dUTPase activity in human cells. Transfection of the siRNA into HeLa and HT29 cells resulted in a 56 +/- 3.6% decrease in dUTPase activity, while transfection of SW620 cells resulted in a 27 +/- 6% decrease in dUTPase activity when compared to non-treated controls.
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Williams
authors.forename: A W
authors.initials: AW
authors.lastname: Studebaker
mesh_terms: D020816:Amino Acid Motifs; D015536:Down-Regulation; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D011755:Pyrophosphatases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D013997:Time Factors; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase