title: Transglycosylation activity of Dictyoglomus thermophilum amylase A.
abstract: Amylase A from Dictyoglomus thermophilum is a thermophilic enzyme and has about 40% identity with 4-alpha-glucanotransferase (GTase) from Thermococcus litoralis, and both of these enzymes belong to family 57 glycosyl hydrolase. Since the transglycosylation activity of T. litoralis GTase has been well characterized, the substrate specificity and reaction products of amylase A from D. thermophilum were examined. alpha-1,4 Glucan was produced from maltooligosaccharides, and glucoamylase-resistant molecules (cycloamyloses) were produced from longer chain amylose (average molecular mass 200 kDa). It has been reported that amylase A from D. thermophilum hydrolyzes starch, but in this study it was found that the enzyme was also able to use maltooligosaccharides and long chain amylose as substrate and has transglycosylation activity.
authors.affiliation: Department of Biotechnology, University of Tokyo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Hiromi
authors.initials: H
authors.lastname: Imamura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
mesh_terms: D000681:Amylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000201:enzymology; D005936:Glucans / Q000378:metabolism; D006004:Glycogen Debranching Enzyme System / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D006868:Hydrolysis; D009844:Oligosaccharides / Q000378:metabolism; D013213:Starch / Q000737:chemistry; D014312:Trisaccharides / Q000378:metabolism
chemical_list: D005936:Glucans; D006004:Glycogen Debranching Enzyme System; D009844:Oligosaccharides; D014312:Trisaccharides; C021705:maltooligosaccharides; C008317:maltotriose; D013213:Starch; C020569:4 alpha-glucanotransferase; D000681:Amylases