title: Lack of association of cytomegalovirus with human brain tumors.
abstract: Cytomegalovirus (CMV) is thought to possess oncogenic properties and has been linked with a number of human malignancies. CMV infection was recently described in association with malignant gliomas. The intent of the present study was to further investigate the reported association between CMV and malignant gliomas. Tissue from 22 brain tumors of various histologic types and grades, four normal brains, six breast carcinomas, six colon carcinomas, six lung carcinomas, and six sarcomas were evaluated for the presence of CMV by polymerase chain reaction (PCR), in situ hybridization, and immunohistochemical methods. None of the brain tumors or normal brain tissue tested demonstrated evidence of CMV pp65 or early nuclear proteins by immunohistochemistry. In addition, no CMV RNA or DNA was detected in these cases by in situ hybridization and PCR. None of the carcinomas or sarcomas evaluated were positive for CMV by immunohistochemistry, in situ hybridization, or PCR. The findings of the present study suggest that CMV is not significantly associated with brain tumors in humans.
authors.affiliation: Department of Pathology, City of Hope National Medical Center, Duarte, CA 91010, USA. SLau@coh.org
authors.forename: Sean K
authors.initials: SK
authors.lastname: Lau
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Chen
authors.forename: Wen-Gang
authors.initials: WG
authors.lastname: Chen
authors.forename: Don J
authors.initials: DJ
authors.lastname: Diamond
authors.forename: Adam N
authors.initials: AN
authors.lastname: Mamelak
authors.forename: John A
authors.initials: JA
authors.lastname: Zaia
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Weiss
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology* / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D014764:Viral Proteins / Q000032:analysis
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins