Annoglabayin, a novel dimeric kaurane diterpenoid, and apoptosis in Hep G2 cells of annomontacin from the fruits of Annona glabra.
Annoglabayin (1), a novel Annona dimeric kaurane diterpenoid, has been isolated from Annona glabra, and its structure was determined on the basis of spectroscopic analysis. Annoglabayin (1) contains a unique carbon bridge between two nor-ent-kaurane monomeric units. The dose-response of 2 in Hep G2 cells indicated that 2 increased DNA damage. In addition, our results showed that 2 induced a noticeable decrease in mitochondrial transmembrane potential during treatment. These results indicate that 2 produces apoptotic events in Hep G2 cells, through inducing changes in mitochondria.
Chen
Ching-Hsein
CH
Department of Medical Technology, Fooyin University, Ta-Liao, Kaohsiung 831, Taiwan.
Hsieh
Tian-Jye
TJ
Liu
Tsan-Zon
TZ
Chern
Chi-Liang
CL
Hsieh
Pei-Ying
PY
Chen
Chung-Yi
CY
D031041:Annona / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D013624:Taiwan; D014407:Tumor Cells, Cultured
D045786:Diterpenes, Kaurane; C495014:annoglabayin