The macrophage-activating lipopeptide-2 accelerates wound healing in diabetic mice.
Wound healing in healthy individuals proceeds at an optimal rate. However, in patients, with -- e.g.-- locally impaired blood flow or diabetes, chronic wounds develop and often become infected. Chronic wounds mean a low quality of life for the afflicted patients, not to mention enormous costs. Rather than using recombinant growth factors to accelerate wound healing, we employed the toll-like receptor agonist macrophage-activating lipopeptide-2 (MALP-2) to improve the healing of full-thickness excision skin wounds in an animal model with obese, diabetic mice. A gene array experiment suggested that MALP-2 stimulates the release of various mediators involved in wound healing. Further data to be presented in this study will show (i) that MALP-2 is capable of stimulating the appearance of the monocyte chemoattractant protein-1 at the wound site, (ii) that this leads to increased leucocyte and, in particular, macrophage infiltration and (iii) that MALP-2-treated wounds closed 2 weeks earlier than vehicle-treated controls. MALP-2, thus, appears to stimulate the early inflammatory process needed to set in motion the ensuing consecutive natural steps of wound healing resulting in wound closure.
Deiters
U
U
Wound Healing Research Group, BioTec-Gründerzentrum, Braunschweig, Germany.
Barsig
J
J
Tawil
B
B
Mühlradt
P F
PF
D000818:Animals; D003713:Dendritic Cells / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology*; D005786:Gene Expression Regulation; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D006909:Hydroxyproline / Q000737:chemistry; D007962:Leukocytes / Q000378:metabolism; D055666:Lipopeptides; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides / Q000378:metabolism / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D012867:Skin / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing
D018960:Hyaluronan Receptors; D055666:Lipopeptides; D009842:Oligopeptides; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C106335:macrophage stimulatory lipopeptide 2; D006909:Hydroxyproline