Phorbol ester-induced differentiation of L6 myogenic cells involves phospholipase D activation.
TPA, a potent PKC activator, inhibits myogenic differentiation and activates phospholipase D (PLD). We evaluated the involvement of PLD in the TPA effects on L6 myoblasts differentiation. TPA, at concentrations inhibiting differentiation of L6 cells, induced a strong, though transient, PLD activation. Surprisingly, at nanomolar concentration, TPA induced both myogenic differentiation and sustained activation of PLD. Differential effect of TPA can be ascribed to PKC downregulation induced by highest TPA concentrations. TPA-induced differentiation was inhibited by 1-butanol, confirming the involvement of PLD in this effect. These data suggest that prolonged elevation of PLD activity is required for myogenic differentiation.
Komati
Hiba
H
Laboratoire de Physiopathologie des Lipides et Membranes, Institut National de la Santé et de la Recherche Médicale Unité 585, Institut National des Sciences Appliquées de Lyon, 69621 Villeurbanne, France.
Minasi
Alessandra
A
Naro
Fabio
F
Lagarde
Michel
M
Prigent
Annie-France
AF
Adamo
Sergio
S
Némoz
Georges
G
D020001:1-Butanol / Q000494:pharmacology; D000199:Actins / Q000378:metabolism; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004789:Enzyme Activation; D007700:Kinetics; D032446:Myoblasts / Q000187:drug effects*; D018008:Myogenin / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology*; D010739:Phospholipase D / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000199:Actins; D002273:Carcinogens; C503803:Myog protein, rat; D018008:Myogenin; D010703:Phorbol Esters; D020001:1-Butanol; D011493:Protein Kinase C; D010739:Phospholipase D; D009218:Myosins; D013755:Tetradecanoylphorbol Acetate