PKCdelta is involved in signal attenuation in CD3+ T cells.
PKCdelta has been implicated in the signalling events after engagement of the antigen specific receptor on B cells and the Fc-epsilon receptor on mast cells. Employing our recently established PKCdelta null mice , we here investigate the physiological function of PKCdelta in CD3+ T cells. As result, administration of anti-CD3 antibodies in vivo induced markedly increased blood plasma IL-2 levels (but not IL-4, IFN-gamma, TNF-alpha and IL-6 levels) in the PKCdelta null mice, when compared to wild-type sibling controls. Additionally, in vitro T cell proliferative responses to allogenic MHC are significantly enhanced in PKCdelta-deficient CD3+ T cells. These findings suggest that PKCdelta serves a so far unrecognized role in TCR-induced negative regulation of IL-2 cytokine production and T cell proliferation.
Gruber
Thomas
T
Department of Medical Biology and Human Genetics, Innsbruck Medical University, Schoepfstrasse 41, A-6020 Innsbruck, Austria.
Barsig
Johannes
J
Pfeifhofer
Christa
C
Ghaffari-Tabrizi
Nassim
N
Tinhofer
Inge
I
Leitges
Michael
M
Baier
Gottfried
G
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D017252:CD3 Complex / Q000032:analysis* / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000378:metabolism; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D011493:Protein Kinase C / Q000235:genetics / Q000502:physiology*; D051745:Protein Kinase C-delta; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology*
D000906:Antibodies; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4; C497259:Prkcd protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta