[Establishment of immortalized cell line BLTR-4 and primary identification of its biological character].
OBJECTIVE
To establish immortalized cell line from the urothelium of the urinary bladder and identify the characteristics of the cell line.


METHODS
Human papillomavirus 16 (HPV-16) plasmid was used to transfect urothelium of infant urinary bladder in vitro with the help of Fugene-6, and this plasmid contained E6 and E7 genes of HPV-16. We also identified the existence of HPV-16 E6 and E7 genes and the biological characteristics of the cell line by PCR, immunohistochemistry, and the biology identification.


RESULTS
BLTR-4 cell line, produced from the transfection of HPV-16K plasmid, was a cell line from urothelium with the expression of HPV-16 E6 and E7 genes. It had been cultured more than 70 passages, and the characteristics of growth was similar to the immortalized cell line as reported.


CONCLUSIONS
BLTR-4 cell line is an immortalized cell line from urothelium of the urinary bladder, which contains HPV-16 E6 and E7 genes. BLTR-4 cell line is a good experimental model to investigate the relationship of the infection of high risk HPV and transitional cell carcinoma (TCC) in vitro.
Zheng
Shan
S
Department of Pathology, Cancer Hospital, CAMS and PUMC, Beijing 100021, China.
Guo
Su-ping
SP
He
Zu-gen
ZG
Cheng
Shu-jun
SJ
Gao
Yan-ning
YN
D002461:Cell Line, Transformed; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections / Q000821:virology; D010957:Plasmids / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014412:Tumor Virus Infections / Q000821:virology; D001743:Urinary Bladder / Q000166:cytology*; D001749:Urinary Bladder Neoplasms / Q000821:virology
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16