title: On-line sample cleanup and chiral separation of gemifloxacin in a urinary solution using chiral crown ether as a chiral selector in microchip electrophoresis.
abstract: In chiral capillary electrophoresis of primary amine enantiomers using (+)-18-crown-6-tetracarboxylic acid (18C6H4) as a chiral selector, the presence of alkaline metal ions in the sample solution as well as in the run buffer is undesirable due to their strong competitive binding with 18C6H4. A channel-coupled microchip electrophoresis device was designed to clean up alkaline metal ions from a sample matrix for the chiral analysis of amine. In the first channel, the metal ions in the sample were monitored by indirect detection using quinine as a chromophore and drained to the waste. In the second separation channel, gemifloxacin enantiomers, free of the alkaline metal ions, were successfully separated using only a small amount of the chiral selector (50 microM 18C6H4).
authors.affiliation: School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, South Korea.
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Cho
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Shim
authors.forename: Min-Su
authors.initials: MS
authors.lastname: Kim
authors.forename: Yong-Kweon
authors.initials: YK
authors.lastname: Kim
authors.forename: Doo Soo
authors.initials: DS
authors.lastname: Chung
mesh_terms: D000900:Anti-Bacterial Agents / Q000652:urine*; D043844:Crown Ethers / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D024841:Fluoroquinolones / Q000652:urine*; D000077735:Gemifloxacin; D008904:Miniaturization; D009287:Naphthyridines / Q000652:urine*; D013237:Stereoisomerism
chemical_list: D000900:Anti-Bacterial Agents; D043844:Crown Ethers; D024841:Fluoroquinolones; D009287:Naphthyridines; D000077735:Gemifloxacin