Evaluation of the role of constitutive isocitrate lyase activity in Yersinia pestis infection of the flea vector and mammalian host.
Yersinia pestis, unlike the closely related Yersinia pseudotuberculosis, constitutively produces isocitrate lyase (ICL). Here we show that the Y. pestis aceA homologue encodes ICL and is required for growth on acetate but not for flea infection or virulence in mice. Thus, deregulation of the glyoxylate pathway does not underlie the recent adaptation of Y. pestis to arthropod-borne transmission.
Sebbane
Florent
F
Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases/NIH, 903 S. 4th St., Hamilton, MT 59840, USA.
Jarrett
Clayton O
CO
Linkenhoker
Jan R
JR
Hinnebusch
B Joseph
BJ
D000818:Animals; D007303:Insect Vectors / Q000382:microbiology*; D007522:Isocitrate Lyase / Q000502:physiology*; D051379:Mice; D005423:Siphonaptera / Q000382:microbiology*; D014774:Virulence; D015010:Yersinia pestis / Q000201:enzymology* / Q000472:pathogenicity*; D015011:Yersinia pseudotuberculosis / Q000472:pathogenicity
D007522:Isocitrate Lyase