title: Measurement of the carbohydrate-binding specificity of lectins by a multiplexed bead-based flow cytometric assay.
abstract: Carbohydrate binding underlies many cell recognition events. Here, we describe a multiplexed glyco-bead array method for determining the carbohydrate-binding specificities of plant lectins using a bead-based flow cytometric analysis. N-glycans including high mannose, hybrid, and complex types and O-glycans from glycoproteins were immobilized on multiplexed beads, and the specificities of 13 kinds of sugar chains were monitored within 2 h in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples and allows the reliable and simultaneous elucidation of sugar-binding properties under identical conditions.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8562 Chiba, Japan. yamamoto@k.u-tokyo.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Ito
authors.forename: Fumiko
authors.initials: F
authors.lastname: Yasukawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Konami
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsumoto
mesh_terms: D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006020:Glycopeptides / Q000378:metabolism*; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000032:analysis / Q000378:metabolism; D008863:Microspheres; D012680:Sensitivity and Specificity
chemical_list: D006020:Glycopeptides; D037102:Lectins; D037241:Mannose-Binding Lectins