Analysis of Cell Migration in Caenorhabditis elegans.
This chapter is concerned with a method of analysis and quantification of cell migration defects in mutants of the nematode worm Caenorhabditis elegans. The method takes advantage of transgenic expression of the green fluorescent protein to visualize migrating cells. By following these protocols, one will be able to analyze cell migration defects in new mutant strains for comparison to wild-type and to other mutants. Techniques described include obtaining wild-type and mutant worm strains as well as strains harboring green fluorescent protein transgenes; maintenance and manipulation of C. elegans in the laboratory; introducing transgenes into different genetic backgrounds; mounting worms for fluorescence microscopy; and scoring and analysis of cell migration defects.
Shakir
M Afaq
MA
Department of Molecular Biosciences, University of Kansas, Lawrence, KS, USA.
Lundquist
Erik A
EA
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000166:cytology / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D003433:Crosses, Genetic; D005260:Female; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014162:Transfection / Q000379:methods
D049452:Green Fluorescent Proteins