[Establishment and evaluation of the method for removing albumin and IgG in serum proteomics].
OBJECTIVE
To establish and evaluate the method for removing albumin and IgG in serum proteomics.


METHODS
Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE).


RESULTS
The method for removing serum albumin and IgG was established, which enabled visualization of low-abundance proteins better in 2-DE map.


CONCLUSIONS
The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases.
Li
Xue-hua
XH
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. snow110@fimmu.com
Li
Xin
X
Xu
Shou-jun
SJ
Ding
Yan-qing
YQ
D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012709:Serum Albumin / Q000302:isolation & purification*
D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin