[In vitro effect of interferon alpha on proliferation of human marrow fibroblast].
OBJECTIVE
To explore the effect of interferon alpha (IFN-alpha) on proliferation of human marrow fibroblasts.


METHODS
Marrow fibroblasts were isolated from patients with iron deficiency anemia. Platelet derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1) or INF-alpha2b was added in the cell cultures each alone or in combinations. Cell proliferation and cell cycle of fibroblasts were detected by MTT and flow cytometry, respectively.


RESULTS
PDGF-ABqa (1 approximately 50 ng/ml) or TGF-beta1 (0.01 approximately 1 ng/ml) could stimulate the proliferation of the fibroblasts, while PDGF-AB was more effective (P < 0.01). IFN-alpha2b partially suppressed PDGF-AB induced proliferation of fibroblasts,the inhibition rate was 7.62%. IFN-alpha2b and TGF-beta1 combination suppressed synergistically not only the proliferation of the fibroblasts (P < 0.01) but also the PDGF-AB induced-proliferation (P < 0.01) at S phase.


CONCLUSIONS
IFN-alpha2b combined with TGF-beta1 could synergistically suppress the PDGF-AB induced proliferation of fibroblasts at S phase in vitro.
Huang
Jin-wen
JW
Department of Hematology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, China.
Xu
Jing
J
D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta