Expression levels of protein kinase C-alpha in non-small-cell lung cancer.
Current treatments of non-small-cell lung cancer (NSCLC) are inadequate and new therapies are being developed that target specific cellular signaling proteins associated with tumor growth. One potential target is protein kinase C (PKC)-alpha, a signaling molecule with an important role in cell regulation and proliferation. The present study examines the expression levels of PKC-alpha in NSCLC to better understand the distribution of PKC-alpha in NSCLC. We analyzed tumor specimens from an independent tumor tissue bank to determine PKC-alpha protein and messenger RNA gene expression in NSCLC. In addition, we used publicly available gene expression array data to further understand PKC-a-associated gene expression profiles in NSCLC. We found that PKC-alpha is highly expressed in < or = 20% of patients with NSCLC. We also found that PKC-alpha was preferentially expressed in adenocarcinoma compared with squamous cell carcinoma of the lung.
Lahn
Michael
M
Oncology Product Development, Eli Lilly and Company, Indianapolis, IN 46285, USA. mlahn@lilly.com
Su
Chen
C
Li
Shuyu
S
Chedid
Marcio
M
Hanna
Kimberly R
KR
Graff
Jeremy R
JR
Sandusky
George E
GE
Ma
Doreen
D
Niyikiza
Clet
C
Sundell
Karen L
KL
John
William J
WJ
Giordano
Thomas J
TJ
Beer
David G
DG
Paterson
Blake M
BM
Su
Eric Wen
EW
Bumol
Thomas F
TF
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011493:Protein Kinase C / Q000235:genetics*; D051571:Protein Kinase C-alpha; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha