[Screening, cloning, and analyzing for hSNF5 binding proteins in human fetal brain].
OBJECTIVE
To identify novel binding proteins of hSNF5, a subunit of chromatin remodeling complex in human fetal brain.


METHODS
The yeast two-hybrid system was used for this study. Positive cDNA clones were sequenced. Sequence homology and putative functional domains were analyzed and compared with databank.


RESULTS
Nine positive clones obtained were analyzed, among which the sequence of one clone was 97% homologous to the 3' mRNA of a hypothetical protein FLJ20643, while other four clones were related to protein coding sequences existed in the GenBank. The rest four clones were not in frame with any known protein coding sequence.


CONCLUSIONS
Clones encoding for hSNF5 binding protein exists in cDNA library of human brain. These proteins may recruit chromatin remodeling complex via hSNF5 to modulate the transcription of their target gene and the related cell functions.
Zhang
Yi
Y
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Yang
Jun
J
Wu
Ning-hua
NH
Shen
Yu-fei
YF
D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D004622:Embryo, Mammalian; D006801:Humans; D000071796:SMARCB1 Protein; D015534:Trans-Activators; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D015534:Trans-Activators; D014157:Transcription Factors