title: Intranasal vaccination with streptococcal fibronectin binding protein Sfb1 fails to prevent growth and dissemination of Streptococcus pyogenes in a murine skin infection model.
abstract: Fibronectin binding protein F1 (Sfb1) of Streptococcus pyogenes (group A streptococcus [GAS]) is a well-characterized adhesin that has been shown to induce protection in mice against a lethal intranasal GAS challenge after intranasal immunization with cholera toxin B subunit (CTB) as adjuvant. With a murine skin infection model, we have shown that Sfb1/CTB vaccination neither elicits opsonizing antibodies nor prevents systemic bacterial growth and dissemination to internal organs after a subcutaneous GAS challenge. These results indicate that an Sfb1-based vaccine should be complemented with additional protective antigens in order to be used in areas such as the tropical north of Australia, where the skin is the primary route of entry for invasive streptococcal diseases.
authors.affiliation: Department of Biological Sciences, University of Wollongong, Wollongong, N.S.W. 2522, Australia. mwalker@uow.edu.au.
authors.forename: J
authors.initials: J
authors.lastname: McArthur
authors.forename: E
authors.initials: E
authors.lastname: Medina
authors.forename: A
authors.initials: A
authors.lastname: Mueller
authors.forename: J
authors.initials: J
authors.lastname: Chin
authors.forename: B J
authors.initials: BJ
authors.lastname: Currie
authors.forename: K S
authors.initials: KS
authors.lastname: Sriprakash
authors.forename: S R
authors.initials: SR
authors.lastname: Talay
authors.forename: G S
authors.initials: GS
authors.lastname: Chhatwal
authors.forename: M J
authors.initials: MJ
authors.lastname: Walker
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001770:Blood Bactericidal Activity; D004195:Disease Models, Animal; D051379:Mice; D008807:Mice, Inbred BALB C; D017192:Skin Diseases, Bacterial / Q000517:prevention & control*; D013290:Streptococcal Infections / Q000517:prevention & control*; D013297:Streptococcus pyogenes / Q000276:immunology*; D014611:Vaccination
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; C078635:fibronectin-binding proteins, bacterial