title: Pivotal participation of nitrogen dioxide in L-arginine induced acute necrotizing pancreatitis: protective role of superoxide scavenger 4-OH-TEMPO.
abstract: For the first time, a direct sensitive method of *NO(2) detection and measurement in biological material has been established. It is based on the interaction of this radical with the coordination compound of Cr(III) with aminodeoxysugar as biosensor. Our new method makes it possible to precisely assess *NO(2) level in experimental acute necrotizing pancreatitis induced by L-arginine, where oxidative and nitrosative stresses are supposed to play a key role in the pathomechanism of the disease. As much as 20 nmol of *NO(2)/mg protein was detected which correlated with severe deterioration of pancreatic acinar cell ultrastructure. Protective effect of superoxide radical scavenger 4-OH-TEMPO expressed as *NO(2) level decrease confirmed by preserved acinar cell ultrastructure and decreased pancreatic amylase release to blood serum is demonstrated. This study reveals a possible pathomechanism of L-arginine induced acute pancreatitis.
authors.affiliation: Department of Chemistry, University of Gdańsk, Sobieskiego 18/19, 80-952 Gdańsk, Poland. alex@chemik.chem.univ.gda.pl <alex@chemik.chem.univ.gda.pl>
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Dabrowska
authors.forename: Dagmara
authors.initials: D
authors.lastname: Jacewicz
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Łapińska
authors.forename: Bogdan
authors.initials: B
authors.lastname: Banecki
authors.forename: Adam
authors.initials: A
authors.lastname: Figarski
authors.forename: Michał
authors.initials: M
authors.lastname: Szkatuła
authors.forename: Jedrzej
authors.initials: J
authors.lastname: Lehman
authors.forename: Jacek
authors.initials: J
authors.lastname: Krajewski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kubasik-Juraniec
authors.forename: Michał
authors.initials: M
authors.lastname: Woźniak
authors.forename: Lech
authors.initials: L
authors.lastname: Chmurzyński
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008854:Microscopy, Electron; D015394:Molecular Structure; D009585:Nitrogen Dioxide / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D013057:Spectrum Analysis; D013113:Spin Labels; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
chemical_list: D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D013113:Spin Labels; D013438:Sulfhydryl Compounds; D013481:Superoxides; D001120:Arginine; D000681:Amylases; D009585:Nitrogen Dioxide; C001803:tempol