[Experimental study on the fabrication of bioactive membrane for inducing bone regeneration].
The aim of this study was to develop a bioactive membrane for inducing bone regeneration. The membrane was composed of polylactic acid, collagen, recombinant human bone morphogenetic protein-2 (rhBMP-2). The PLA + collagen + rhBMP-2 membrane was fabricated by solvent-casting and cool-drying. The mechanic properties of this compound membrane were tested. The two surfaces of membrane were observed by SEM. Degradability of PLA was evaluated by SEM observation and molecular weight measure in vitro and in vivo. The compound membranes were implanted in rabbit muscles. The samples were obtained when animals were sacrificed at different periods: 2 weeks, 1, 2, 3, 6 months after surgery. The biodegradability and biocompatibility of the membrane were evaluated. The heterotopic bone inducing activity of BMP was identified. The results indicated that the strength at extension to failure of the compound membrane was 36.4MPa at 2.3% strain. The compound membrane was found bearing active factor on its coarse side, which can induce bone regeneration. After implantation in vivo, the membrane maintained the structure for three months and degraded in 6 months. Based on histological analysis, there was no obvious inflammation. Heterotopic bone was induced. We could conclude that the PLA + collagen + rhBMP-2 membrane is an absorbable compound membrane that possesses good biocompatibility, adequate mechanic properties and excellent property of bone induction. It could be applied as an ideal membrane for inducing bone regeneration.
Tian
Weidong
W
West China College of Stomatology, Sichuan University, Chengdu 610041, China. doubean1@sina.com
Bao
Chongyun
C
Liu
Lei
L
Tang
Wei
W
Zheng
Xiaohui
X
Li
Shengwei
S
Xiong
Chengdong
C
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage* / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000008:administration & dosage* / Q000494:pharmacology; D048091:Guided Tissue Regeneration; D019344:Lactic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage* / Q000494:pharmacology; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000008:administration & dosage* / Q000494:pharmacology
C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D016212:Transforming Growth Factor beta; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen