Ensemble hybridisation--a new method for exploring sequence dependent fluorescence of dye-nucleic acid conjugates.
The fluorescence of thiazole orange as artificial base in PNA was investigated in a nearest neighbour analysis; library-to-library hybridisation allowed the identification of probe sequences suitable for homogeneous DNA detection.
Köhler
Olaf
O
Institut für Chemie, Humboldt-Universität zu Berlin, Brook-Taylor-Strasse 2, D-12489 Berlin, Germany.
Jarikote
Dilip Venkatrao
DV
Seitz
Oliver
O
D052160:Benzothiazoles; D005456:Fluorescent Dyes / Q000737:chemistry*; D015723:Gene Library; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011804:Quinolines; D013844:Thiazoles / Q000737:chemistry*
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D011804:Quinolines; D013844:Thiazoles; C051000:thiazole orange