[Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui].
OBJECTIVE
To select the sodium chloride tolerant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment.


METHODS
Calli were induced from the leaves of C. morifolium. The calli were treated with 0.2% and 0.5% EMS, respectively. After 15 days' culture, the calli were transplanted to selection media with 0.5%, 1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out.


CONCLUSIONS
After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed.
Xue
Jian-ping
JP
Department of Biology, Huaibei Coal Industry Teachers' College, Huaibei 235000, China. xuejp2000@yahoo.com.cn
Zhang
Ai-ming
AM
Gao
Xiang
X
Sheng
Wei
W
D031202:Chrysanthemum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D009154:Mutation; D018515:Plant Leaves / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D012965:Sodium Chloride / Q000494:pharmacology*
D003470:Culture Media; D012965:Sodium Chloride