Relationship between the action of reactive oxygen and nitrogen species on bilayer membranes and antioxidants.
Membrane lipid peroxidation (LPO) induced by hydroxyl (*OH) and ascorbyl (*Asc) radicals and by peroxynitrite (ONOO-) was investigated in asolectin (ASO), egg phosphatidylcholine (PC) and PC/phosphatidic acid mixtures (PC:PA) liposomes and rat liver microsomes (MC). Enthalpy variation (DeltaH) of PC:PA at different molar ratios were obtained by differential scanning calorimetry. It was also evaluated the LPO inhibition by quercetin, melatonin and Vitamin B6. The oxidant effect power follows the order *OH approximately *Asc > ONOO- on PC and MC; whilst on ASO liposomes, it follows *Asc > *OH approximately ONOO-. Increasing amounts of PA in PC liposomes resulted in lower levels of LPO. The DeltaH values indicate a more ordered membrane arrangement as a function of PA amount. The results were discussed in order to provide a complete view involving the influence of membranes, oxidants and antioxidants intrinsic behavior on the LPO dynamics.
de Lima
Vânia Rodrigues
VR
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, C.P. 476, CEP 88040-900 Florianópolis, Brazil.
Morfim
Marcos Paulo
MP
Teixeira
Adriana
A
Creczynski-Pasa
Tânia Beatriz
TB
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D004530:Egg Yolk / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D008862:Microsomes, Liver / Q000737:chemistry / Q000378:metabolism; D010712:Phosphatidic Acids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D051381:Rats; D026361:Reactive Nitrogen Species / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
D000975:Antioxidants; D005609:Free Radicals; D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; C024990:asolectin