Expression of a beta-defensin mRNA, lingual antimicrobial peptide, in bovine mammary epithelial tissue is induced by mastitis.
The expression of a beta-defensin, the lingual antimicrobial peptide (LAP), in response to mastitis was investigated by real-time PCR of RNA from mastitic and control udder quarters. There was a positive relationship between somatic cell count in milk and LAP expression. In situ hybridization showed that LAP mRNA was expressed in epithelial cells of mastitic tissue. These results suggest that LAP plays a role in the innate immune response to mastitis.
Swanson
Kara
K
Dairy Biotechnology, AgResearch, Hamilton, New Zealand. kara.swanson@agresearch.co.nz
Gorodetsky
Stas
S
Good
Laura
L
Davis
Stephen
S
Musgrave
David
D
Stelwagen
Kerst
K
Farr
Vicki
V
Molenaar
Adrian
A
D000818:Animals; D002417:Cattle; D002452:Cell Count; D005260:Female; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D023083:beta-Defensins / Q000235:genetics*
D012333:RNA, Messenger; D023083:beta-Defensins