[The effects of growth hormone on rabbit's mandibular condylar chondrocytes proliferation and secretion in vitro].
OBJECTIVE
To study the effect of growth hormone (GH) on the proliferation and type II collagen secretion of chondrocytes of mandibular condyle in rabbit in vitro.


METHODS
Flow cytometry (FCM) and immunohistochemical technique were employed to observe the possible changes.


RESULTS
(1) The exogenic GH can enhance the proliferation and synthesis of DNA of the chondrocytes of mandibular condyle in rabbit in vitro. The suitable concentration of GH is 10 microg/ml. The synthesis of DNA reaches the highest level after 12 hours, while the proliferation index (PI) hits the highest after 24 hours. (2) GH (10 microg/ml) can stimulate the secretion of type II collagen of the chondrocytes.


CONCLUSIONS
The exogenic GH can enhance the proliferation, the synthesis of DNA and the secretion of type II collagen of the chondrocytes of mandibular condyle in rabbit in vitro.
Huang
Ning
N
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Luo
Song-jiao
SJ
Yang
Hong-mei
HM
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism*; D024043:Collagen Type II / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D008335:Mandibular Condyle / Q000166:cytology*; D011817:Rabbits
D024043:Collagen Type II; D013006:Growth Hormone