title: Temporal patterns of the cerebral inflammatory response in the rat lithium-pilocarpine model of temporal lobe epilepsy.
abstract: To better understand the role of inflammatory responses in temporal lobe epilepsy, we characterized Interleukin1-beta (IL1-beta), Nuclear Factor-kappaB (NF-kappaB), and Cyclooxygenase-2 (COX-2) expression together with neurodegeneration in the rat lithium-pilocarpine model. The immunohistochemical expression of IL1-beta, NF-kappaB, and COX-2 started by 12 h post-injection, persisted for 24 h (status epilepticus period), and returned to basal levels by 3 and 6 days (latent period). The regional distribution of IL1-beta, NF-kappaB, and COX-2 occurred mainly in structures prone to develop neuronal damage. Using double-staining protocols, we detected IL1-beta expression in glial cells, COX-2 expression in neurons, and NF-kappaB in both cell types. The presence of Fluoro-Jade-B-positive degenerating neurons was associated with IL1-beta, NF-kappaB, and COX-2 proteins expression during status epilepticus but not during the latent period while neurons were still degenerating. These data suggest that seizure-related IL1-beta, NF-kappaB, and COX-2 expression may contribute to the pathophysiology of epilepsy by inducing neuronal death and astrocytic activation.
authors.affiliation: INSERM U405, Faculty of Medicine, Strasbourg, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Voutsinos-Porche
authors.forename: Estelle
authors.initials: E
authors.lastname: Koning
authors.forename: Hervé
authors.initials: H
authors.lastname: Kaplan
authors.forename: Arielle
authors.initials: A
authors.lastname: Ferrandon
authors.forename: Moncef
authors.initials: M
authors.lastname: Guenounou
authors.forename: Astrid
authors.initials: A
authors.lastname: Nehlig
authors.forename: Jacques
authors.initials: J
authors.lastname: Motte
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D007249:Inflammation; D007375:Interleukin-1 / Q000378:metabolism; D007700:Kinetics; D008094:Lithium; D016328:NF-kappa B / Q000378:metabolism; D010862:Pilocarpine; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000503:physiopathology
chemical_list: D007375:Interleukin-1; D016328:NF-kappa B; D010862:Pilocarpine; D008094:Lithium; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases