title: Inner core assembly and structure of the lipooligosaccharide of Neisseria meningitidis: capacity of strain NMB to express all known immunotype epitopes.
abstract: Neisseria meningitidis expresses a heterogeneous population of lipooligosaccharide (LOS) inner cores variously substituted with alpha1-3-linked glucose and O-3, O-6, and O-7 linked phosphoethanolamine (PEA), as well as glycine, attached to HepII. Combinations of these attachments to the LOS inner core represent immunodominant epitopes that are being exploited as future vaccine candidates. Historically, each LOS immunotype was structurally assessed and prescribed a certain unique inner core epitope. We report that a single isolate, strain NMB, possesses the capacity to produce all of the known neisserial LOS inner core immunotype structures. Analysis of the inner cores from parental LOS revealed the presence or absence of alpha1,3-linked glucose, O-6 and/or O-7 linked PEA, in addition to glycine attached at the 7 position of the HepII inner core. Identification and inactivation of lpt-6 in strain NMB resulted in the loss of both O-6 and O-7 linked PEA groups from the LOS inner core, suggesting that Lpt-6 of strain NMB may have bifunctional transferase activities or that the O-6 linked PEA groups once attached to the inner core undergo nonenzymatic transfer to the O-7 position of HepII. Although O-3 linked PEA was not detected in parental LOS inner cores devoid of alpha1-3-linked glucose residues, LOS glycoforms bearing O-3 PEA groups accumulated in a truncated mutant, NMBlgtK (Hep2Kdo2-lipid A). Because these structures disappeared upon inactivation of the lpt-3 locus, strain NMB expresses a functional O-3 PEA transferase. The LOS glycoforms expressed by NMBlgtK were also devoid of glycine attachments, indicating that glycine was added to the inner core after the completion of the gamma-chain by LgtK. In conclusion, strain NMB has the capability to express all known inner core structures, but in in vitro culture L2 and L4 immunotype structures are predominantly expressed.
authors.affiliation: Department of Microbiology, Monash University, Clayton 3800, Australia. charlene.kahler@med.monoash.edu.au
authors.forename: Charlene M
authors.initials: CM
authors.lastname: Kahler
authors.forename: Anup
authors.initials: A
authors.lastname: Datta
authors.forename: Yih-ling
authors.initials: YL
authors.lastname: Tzeng
authors.forename: Russell W
authors.initials: RW
authors.lastname: Carlson
authors.forename: David S
authors.initials: DS
authors.lastname: Stephens
mesh_terms: D002240:Carbohydrate Sequence; D000939:Epitopes / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000939:Epitopes; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides