A role for beta-actin in RNA polymerase III transcription.
When transcription from the human U6 snRNA gene is reconstituted with recombinant factors and purified RNA polymerase III (pol III), pol III must be treated with CK2 to be active. We show that highly purified pol III contains associated beta-actin, and beta-actin localizes to an active U6 promoter in vivo. Pol III immunoprecipitated from IMR90 cells treated with a genotoxic agent lacks associated beta-actin and is inactive in the reconstituted assay. Transcription is regained upon treatment of pol III with CK2 and addition of beta-actin. This suggests that beta-actin associated with pol III is essential for basal pol III transcription.
Hu
Ping
P
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Wu
Si
S
Hernandez
Nouria
N
D000199:Actins / Q000378:metabolism*; D047390:Casein Kinase II / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D011401:Promoter Regions, Genetic / Q000235:genetics; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D000199:Actins; D012342:RNA, Small Nuclear; C405486:U6 small nuclear RNA; D047390:Casein Kinase II; D012320:RNA Polymerase III