[Effects of cyclosporine A on NIT-1 beta cell proliferation and pol alpha1 gene expression in vitro].
OBJECTIVE
To investigate the effects of cyclosporine A on the proliferation and pol alpha1 mRNA expression of cultured NIT-1 beta cells.


METHODS
After exposure to cyclosporine A at various concentrations (0.05 to 10 micromol/L) for 48 h and 72 h, NIT-1 cell proliferation was analyzed by MTT assay and the gene expression determined by reverse transcriptional PCR (RT-PCR).


RESULTS
Forty-eight-hour and 72-hour cyclosporine A exposure inhibited the cell proliferation in a concentration- dependent manner, and at the concentration of 10 micromol/L, cyclosporine A also decreased pol alpha1 mRNA expression after a 48-hour exposure.


CONCLUSIONS
Cyclosporine A can effectively inhibit the proliferation of NIT-1 cells possibly through down-regulating the expression of pol alpha1 mRNA.
Yu
Le
L
Institute of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China. yule@fimmu.com
Lei
Lin-sheng
LS
Wu
Shu-guang
SG
D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D016572:Cyclosporine / Q000494:pharmacology*; D004256:DNA Polymerase I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D012333:RNA, Messenger; D016572:Cyclosporine; D004256:DNA Polymerase I