Coexpression of preprotachykinin A and B transcripts in the bovine corpus luteum and evidence for functional neurokinin receptor activity in luteal endothelial cells and ovarian macrophages.
Nonneuronal cell sources of tachykinins, such as substance P (SP) and neurokinin B (NKB), have been demonstrated in leukocytes, endothelial cells and endocrine cells, and may play a role in corpus luteum (CL) development. For this reason, we analyzed mRNA presence for the two tachykinin precursors together with the neurokinin-1 receptor and the neurokinin-3 receptor (NK-1R and NK-3R, preferred by SP and NKB, respectively) in bovine CL at various stages in the luteal phase. Using the RT-PCR technique, we detected coexpression for the preprotachykinin A gene (PPT-A), which encodes SP and neurokinin A (NKA), and the preprotachykinin B gene (PPT-B) for NKB in the CL at the development, secretion and regression stages. Coexpression was also noted for NK-1R and NK-3R gene transcripts. Cultures of endothelial cells (ECs) derived from bovine CL expressed NK-1R and NK-3R mRNA, as did ovarian macrophages. Agonist treatment induced a stronger intracellular calcium ([Ca2+]i) increase after activation of NK-1R compared to NK-3R, a result that we verified by calcium imaging. This is the first evidence for functional tachykinin receptor activity in luteal ECs and ovarian macrophages from bovine CL.
Brylla
Elke
E
Institute of Anatomy, University of Leipzig, Liebigstrasse 13, D-04103 Leipzig, Germany. elke.brylla@medizin.uni-leipzig.de
Aust
Gabriela
G
Geyer
Maren
M
Uckermann
Ortrud
O
Löffler
Sabine
S
Spanel-Borowski
Katharina
K
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D003338:Corpus Luteum / Q000378:metabolism*; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D018042:Receptors, Neurokinin-3 / Q000378:metabolism; D018039:Receptors, Tachykinin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015320:Tachykinins / Q000096:biosynthesis*; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology
D017931:DNA Primers; D018076:DNA, Complementary; D011498:Protein Precursors; D012333:RNA, Messenger; D018042:Receptors, Neurokinin-3; D018039:Receptors, Tachykinin; D015320:Tachykinins; C044206:preprotachykinin; D012313:RNA; D002118:Calcium