title: New evidences for a regulation of deoxycytidine kinase activity by reversible phosphorylation.
abstract: Recent studies indicate that deoxycytidine kinase (dCK), which activates various nucleoside analogues used in antileukemic therapy, can be regulated by post-translational modification, most probably through reversible phosphorylation. To further unravel its regulation, dCK was overexpressed in HEK-293 cells as a His-tag fusion protein. Western blot analysis showed that purified overexpressed dCK appears as doublet protein bands. The slower band disappeared after treatment with protein phosphatase lambda (PP lambda) in parallel with a decrease of dCK activity, providing additional arguments in favor of both phosphorylated and unphosphorylated forms of dCK.
authors.affiliation: Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology, Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Smal
authors.forename: L
authors.initials: L
authors.lastname: Bertrand
authors.forename: E
authors.initials: E
authors.lastname: Van den Neste
authors.forename: S
authors.initials: S
authors.lastname: Cardoen
authors.forename: M
authors.initials: M
authors.lastname: Veiga-da-Cunha
authors.forename: S
authors.initials: S
authors.lastname: Marie
authors.forename: V
authors.initials: V
authors.lastname: Race
authors.forename: A
authors.initials: A
authors.lastname: Ferrant
authors.forename: G
authors.initials: G
authors.lastname: Van den Berghe
authors.forename: F
authors.initials: F
authors.lastname: Bontemps
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D018076:DNA, Complementary / Q000378:metabolism; D003842:Deoxycytidine Kinase / Q000096:biosynthesis*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D003842:Deoxycytidine Kinase