Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry.
A study of the equine phase II metabolism of the anabolic agent boldenone is reported. Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix. Solid phase extraction was used to purify the analytes, which were then detected by ion trap LC/MS. Negative and positive ionisation mode MS(2) were used for the detection of sulfate and glucuronide conjugates, respectively. Boldenone sulfate and 17-epiboldenone glucuronide were detected as the major and minor phase II metabolites, respectively, in horse urine samples collected following the administration of boldenone undecylenate by intramuscular injection.
Pu
Fan
F
School of Chemistry, F11, University of Sydney, Camperdown, NSW 2006, Australia.
McKinney
Andrew R
AR
Stenhouse
Allen M
AM
Suann
Craig J
CJ
McLeod
Malcolm D
MD
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D020719:Glucuronides / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D013739:Testosterone / Q000031:analogs & derivatives* / Q000652:urine*
D020719:Glucuronides; D013739:Testosterone; C013915:boldenone