title: Plasma prekallikrein/kallikrein processing by lysosomal cysteine proteases.
abstract: Plasma kallikrein plays a role in coagulation, fibrinolysis and inflammation. Cathepsins B and L participate in (patho)physiological processes such as peptide antigen processing, tissue remodeling events, protein turnover in cells, hormone processing and tumor invasion. The present work analyzes the processing of prekallikrein/kallikrein by lysosomal cathepsins. Prekallikrein is not hydrolyzed by catB, and catL generates an inactive fragment of prekallikrein. Both kallikrein chains are hydrolyzed by catL and the light chain is mainly hydrolyzed by catB; kallikrein activity is lower after incubation with catL compared to catB. Our data suggest that the plasma kallikrein/ kinin system can be controlled by cathepsins.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo/EPM, CEP 04044-020, São Paulo, SP, Brazil.
authors.forename: Nilana M T
authors.initials: NM
authors.lastname: Barros
authors.forename: Luciano
authors.initials: L
authors.lastname: Puzer
authors.forename: Ivarne L S
authors.initials: IL
authors.lastname: Tersariol
authors.forename: M Luiza V
authors.initials: ML
authors.lastname: Oliva
authors.forename: Claudio A M
authors.initials: CA
authors.lastname: Sampaio
authors.forename: Adriana K
authors.initials: AK
authors.lastname: Carmona
authors.forename: Guacyara da
authors.initials: G
authors.lastname: Motta
mesh_terms: D002403:Cathepsins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D007610:Kallikreins / Q000097:blood*; D008247:Lysosomes / Q000201:enzymology*; D011288:Prekallikrein / Q000378:metabolism*
chemical_list: D011288:Prekallikrein; D002403:Cathepsins; D007610:Kallikreins