title: [Polymorphonuclear leukocytes decrease the immunostimulatry capacity of human dendritic cells].
abstract: The aim of this work was to evaluate the ability of polymorphonuclear leukocytes (PMNL) to modulate the function of dendritic cells (DCs). Human DCs were used to analyze the effect of PMNL on DCs. The allostimulatory proliferation of T cells induced by DCs incubated with PMNL was lower (42 +/- 14%, n:8, p<0.05) compared with untreated DCs. The decreased proliferation correlated with lower IFNgamma production (DCs: 781 +/- 3 pg/ ml; DCs-CS: 343 +/- 178 pg/ml, p<0.05). The same effect was observed in an antigen specific assay. However, the effect was not observed when PMNL where incubated with mature DCs. Indeed, the effect of PMNL was partially reverted with serine proteases inhibitors. Overall, these results suggest that PMNL modulates DCs activity by serine proteases.
authors.affiliation: Laboratorio de Inmunogenética, Hospital de Clínicas José de San Martín y Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: Paulo
authors.initials: P
authors.lastname: Maffia
authors.forename: Sandra
authors.initials: S
authors.lastname: Zittermann
authors.forename: María Lucila
authors.initials: ML
authors.lastname: Scimone
authors.forename: Fernanda
authors.initials: F
authors.lastname: Buzzola
authors.forename: Nancy
authors.initials: N
authors.lastname: Tateosian
authors.forename: Viviana
authors.initials: V
authors.lastname: Lutzky
authors.forename: Carolina
authors.initials: C
authors.lastname: Jancic
authors.forename: Marcos
authors.initials: M
authors.lastname: Barbosa
authors.forename: Eduardo
authors.initials: E
authors.lastname: Chuluyan
mesh_terms: D015703:Antigens, CD / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D015703:Antigens, CD