A modified Ce/Mg-BCIP-NBT formazan/indigoblue technique for demonstration of non-specific alkaline phosphatase activity.
The wide ranged structurally variability of formazans and their accessibility for auxiliary additives as redoxmediators or metals provide an easy tunable chromogenic visualization technique. We present here an improved nitro blue tetrazolium (NBT) 5-bromo-4-chloro-3-indolyl phosphate (BCIP) method which is superior to the classical McGadey's procedure regarding proper precipitation and localization as well as sensitivity. Different metal additives as well as the overall reaction course modifying additives (redox mediators, chelating additives, buffer) were optimized.
Halbhuber
K J
KJ
Institute of Anatomy/Anatomy II, Friedrich Schiller University Jena, Teichgraben 7, 07743 Jena, Germany. khal@mti-n.uni-jena.de
Krieg
R
R
Geidel
O
O
Dietz
W
W
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002563:Cerium / Q000737:chemistry; D005260:Female; D005562:Formazans / Q000737:chemistry*; D006651:Histocytochemistry / Q000379:methods*; D007203:Indigo Carmine / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D008297:Male; D009580:Nitroblue Tetrazolium / Q000737:chemistry*; D051381:Rats
D005562:Formazans; D007211:Indoles; D009580:Nitroblue Tetrazolium; D002563:Cerium; C035455:5-bromo-4-chloro-3-indoxyl phosphate; D007203:Indigo Carmine; D000469:Alkaline Phosphatase; D008274:Magnesium