Pharmacogenomic genotyping methodologies.
"Personalized medicine" based on an individual's genetic makeup is slowly becoming a reality as pharmacogenomics moves from the research setting to the clinical laboratory. Concordance studies between genotype and phenotype have shown that inherited mutations in several key drug-metabolizing enzymes, such as cytochrome P450 ( CYP ) 2D6 , 2C9 , and 2C19 , result in several distinct phenotypes that lead to different individual responses following drug administration. One of the major driving forces behind pharmacogenomics and its ability to be used effectively are the technologies that are available. A beneficial genotyping test must identify most or all of the mutations that have a significant impact on the expression or function of drug-metabolizing enzymes, transporter proteins, and/or drug receptors. Selection of the appropriate technology will be based on several issues, including prior knowledge of the mutation/polymorphism, sensitivity/specificity, sample requirements, and cost. Since the future volume of pharmacogenomic testing is anticipated to be large, automation of pharmacogenomics will also become increasingly important. This paper provides an overview of current technologies available for assessing polymorphisms on a small- to large-scale basis.
Jannetto
Paul J
PJ
Department of Pathology, Medical College of Wisconsin and Milwaukee County Medical Examiner's Office, Milwaukee, WI, USA.
Laleli-Sahin
Elvan
E
Wong
Steven H
SH
D000483:Alleles; D005821:Genetic Techniques; D005838:Genotype; D006801:Humans; D013058:Mass Spectrometry; D008954:Models, Biological; D009154:Mutation; D036103:Nanotechnology; D010597:Pharmacogenetics / Q000379:methods*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods