title: The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase.
abstract: The pur6 gene of the puromycin biosynthetic gene (pur) cluster from Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking L-tyrosine to both 3'-amino-3'-deoxyadenosine and N6,N6-dimethyl-3'-amino-3'-deoxyadenosine with a peptide-like bond. Identical reactions were performed by cell lysates from Streptomyces lividans or Escherichia coli transformants that expressed pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and O-demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl-aminonucleoside synthetase of the puromycin biosynthetic pathway.
authors.affiliation: Centro de Biología Molecular "Severo Ochoa", CSIC/UAM, Universidad Autónoma, 28049 Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Angel Rubio
authors.forename: Patricia
authors.initials: P
authors.lastname: Barrado
authors.forename: Juan
authors.initials: J
authors.lastname: Carlos Espinosa
authors.forename: Antonio
authors.initials: A
authors.lastname: Jiménez
authors.forename: María
authors.initials: M
authors.lastname: Fernández Lobato
mesh_terms: D002627:Chemistry, Physical / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression; D005798:Genes, Bacterial; D007700:Kinetics; D015394:Molecular Structure; D005810:Multigene Family; D010957:Plasmids; D011691:Puromycin / Q000096:biosynthesis*; D011692:Puromycin Aminonucleoside / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D048170:Streptomyces lividans / Q000235:genetics
chemical_list: D011691:Puromycin; D011692:Puromycin Aminonucleoside