Effects of subcutaneous administration of daidzein on blastocyst implantation in rats.
The study was conducted to investigate the effects of phytoestrogen daidzein on blastocyst implantation in rats. Following successful mating, female rats were given daidzein by subcutaneous administration at the dose of 0 (vehicle control, n=15), 50 mg/kg body weight (n=15) and 150 mg/kg body weight (n=15) daily on day 1-7 of pregnancy and were sacrificed on day 8 of gestation. The results revealed that high-dose treatment (150 mg/kg body weight) significantly diminished the rate of blastocyst implantation and serum levels of gonadotropin-releasing hormone (GnRH), progesterone, and gonadotropins (FSH and LH), meanwhile the serum level of beta endorphin increased significantly. These effects were not observed in the low-dose treatment group (50 mg/kg body weight). The results of this study suggested that the anti-implantation effects of daidzein are probably caused by the interference of the hypothalamus-pituitary-gonadal axis which is involved in the implantation process.
Wu
Zhenlong
Z
Department of Biological Science and Biotechnology, Tsinghua University, Haidian District, Beijing 100084, PR China.
Yang
Ying
Y
Chen
Yue
Y
Xia
Guoliang
G
Zhang
Rongqing
R
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation / Q000187:drug effects*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007987:Gonadotropin-Releasing Hormone / Q000097:blood / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood; D048789:Phytoestrogens / Q000494:pharmacology*; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D007529:Isoflavones; D048789:Phytoestrogens; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; C004742:daidzein; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone