Down-regulation of human deoxyuridine triphosphate nucleotidohydrolase (dUTPase) using small interfering RNA (siRNA).
A small interfering double stranded RNA molecule (siRNA, 21 bp) corresponding to a portion (nucleotides 337 to 357) of domain 3 of the human dUTPase was synthesized and used to determine whether it could down-regulate dUTPase activity in human cells. Transfection of the siRNA into HeLa and HT29 cells resulted in a 56 +/- 3.6% decrease in dUTPase activity, while transfection of SW620 cells resulted in a 27 +/- 6% decrease in dUTPase activity when compared to non-treated controls.
Williams
M V
MV
Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, Ohio 43210, USA.
Studebaker
A W
AW
D020816:Amino Acid Motifs; D015536:Down-Regulation; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D011755:Pyrophosphatases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D013997:Time Factors; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase