Intra-nuclear microtubules and a mitotic spindle orientation checkpoint.
Cells of the fission yeast Schizosaccharomyces pombe have a checkpoint mechanism that reportedly monitors the orientation of the mitotic spindle. Astral microtubules in pre-anaphase spindles are thought to contact the contractile actin ring at the plasma membrane in order to rotate the spindle and to sense spindle orientation. Here, we show that these microtubules are actually inside the nuclear envelope.
Zimmerman
Sabina
S
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA.
Daga
Rafael R
RR
Chang
Fred
F
D000199:Actins / Q000378:metabolism; D000705:Anaphase / Q000502:physiology; D015415:Biomarkers; D022003:Cell Nucleus Structures / Q000378:metabolism* / Q000648:ultrastructure; D016764:Cell Polarity / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D018816:Genes, cdc / Q000502:physiology; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008938:Mitosis / Q000502:physiology*; D009685:Nuclear Envelope / Q000378:metabolism / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins; D012568:Schizosaccharomyces / Q000378:metabolism* / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
D000199:Actins; D015415:Biomarkers; D011993:Recombinant Fusion Proteins; D014404:Tubulin