The influence of surface roughness of titanium on beta1- and beta3-integrin adhesion and the organization of fibronectin in human osteoblastic cells.
Mechanisms of cell adhesion and extracellular matrix formation are primary processes in the interaction with the material surface of an implant which are controlled by integrin receptors. The aim of our study was to find out whether beta1- and beta3-integrins of osteoblastic cells sense the surface topography of titanium, and if structural alterations of integrin adhesions were involved in the organization of fibronectin. Pure titanium surfaces were modified by polishing (P), machining (NT), blasting with glass spheres (GB), and blasting with corundum particles (CB) resulting in increasing roughness. Confocal microscopic investigations revealed fibrillar adhesions of beta1- and alpha5-integrins on P, NT, and GB, but on CB with its sharp edges these integrin subunits did not form fibrillar adhesions. beta3 generally appeared in focal adhesions. We observed aligned fibrillar structures of fibronectin on NT not only on the basal site but interestingly, also on the apical cell surface. In contrast, on CB, fibronectin appeared apically clustered. We suggest that this alignment of fibronectin fibrils depends on the directed actin cytoskeleton and in particular, on the capability of the beta1-integrins to form fibrillar adhesions, which is affected by the surface roughness of titanium.
Lüthen
Frank
F
Department of Internal Medicine, Clinical Research, University of Rostock, Ernst-Heydemann-Str. 6, D-18057 Rostock, Germany.
Lange
Regina
R
Becker
Petra
P
Rychly
Joachim
J
Beck
Ulrich
U
Nebe
J G Barbara
JG
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000327:Adsorption; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D048429:Cell Size; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D006244:Hardness; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D039661:Integrin beta3 / Q000378:metabolism*; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D011485:Protein Binding; D013499:Surface Properties; D014025:Titanium / Q000032:analysis / Q000737:chemistry*
D001672:Biocompatible Materials; D003598:Cytoskeletal Proteins; D005353:Fibronectins; D019012:Integrin beta1; D039661:Integrin beta3; D014025:Titanium