[Expression and DNA binding activity of NF-kappaB in mucosal tissue of chronic rhinosinusitis].
OBJECTIVE
To study the expression and activation of nuclear factor-kappa B (NF-kappaB) in mucosa of rhinosinusitis.


METHODS
The expressions of NF-kappaB p50 and p65 in the mucosa from 18 patients and 10 norms were detected with the method of immunohistochemistry. The activation of DNA-binding proteins which was labelled with 32P-radiolabeled oligonucleotide probe for NF-kappaB was detected with electrophoretic mobility shift assays (EMSA) in mucosa.


RESULTS
Plasma of epithelial and glandular cells displayed a strongly positive staining reaction to p65, and nucleus displayed a strongly positive staining reaction to p50 (7.8%-52.1%). There was significantly difference (chi2 = 22.917, P < 0.01). The DNA-binding proteins activity of 18 samples from patients with chronic rhinosinusitis (28.14 +/- 16.71) was stronger than that (9.28 +/- 2.84) in normal subjects (t = 4.56, P < 0.05).


CONCLUSIONS
The expression and DNA-binding proteins activity of NF-kappaB was enhanced. It indicated that NF-kappaB was activated in mucosal inflammation of chronic rhinosinusitis.
Li
Tong-li
TL
Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital, Sun Yet-sen University, Guangzhou 510630, China.
Li
Yuan
Y
Zhang
Ge-hua
GH
Sun
Ya-nan
YN
Li
Yong-qi
YQ
D016022:Case-Control Studies; D002908:Chronic Disease; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*
D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C498143:RELA protein, human; D051996:Transcription Factor RelA; D004247:DNA