Leptin fluctuates in intestinal ischemia-reperfusion injury as inflammatory cytokine.
As leptin is an active mediator mainly secreted by adipose tissue and is closely related with energy metabolism, we evaluate both the changes of leptin levels in serum and adipose tissue with a concise radioimmunoassay and the changes of leptin mRNA expression in adipose tissue with RT-PCR, during the severe metabolic impediment in rat intestinal ischemia-reperfusion (I/R) injury. Results show that not only leptin levels in serum and adipose tissue but also its mRNA expression in adipose tissue undergo a fluctuation according to different injury times. Therefore, we conclude that leptin has a time-dependent response to acute inflammatory stimuli and acts as an anti-inflammatory cytokine.
Lin
Ji
J
Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, PR China.
Yan
Guang-Tao
GT
Wang
Lu-Huan
LH
Hao
Xiu-Hua
XH
Zhang
Kai
K
Xue
Hui
H
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents; D001483:Base Sequence; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism; D007249:Inflammation; D007422:Intestines / Q000098:blood supply*; D020738:Leptin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism*; D012680:Sensitivity and Specificity
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D020738:Leptin; D012333:RNA, Messenger