Gemcitabine does not prevent acute rejection of the transplanted liver in rats.
Our study was designed to determine effect of gemcitabine on acute rejection of liver in rats. Liver transplantation was performed in rats of the Dark Agouti (DA) and Lewis (LEW) strains. Recipients were divided into three groups: A, DA-to-LEW without immunosuppression; B, DA-to-LEW, treated with cyclosporine A; C, DA-to-LEW, treated with gemcitabine. Immunosuppressants were subcutaneously injected for seven consecutive days after transplantation. On day 7, blood samples and liver graft tissue specimens were harvested. Group A showed severe rejection changes (RAI 8/9); in group B no rejection changes were present (RAI 0/9), and in group C moderate rejection changes were observed (RAI 6/9). Differences were significant between B vs C and A vs C groups; P<0.05. Serum creatinine and urea levels in the gemcitabine group were significantly lower than those in the cyclosporine A group. We did not confirm gemcitabine ability to prevent liver allograft rejection.
Mergental
Hynek
H
Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic. hynek.mergental@seznam.cz
Kriz
Jan
J
Honsova
Eva
E
Kudla
Michal
M
Pantoflicek
Tomas
T
Tcherentsova
Katerina
K
Kocik
Matej
M
Saudek
Frantisek
F
Ryska
Miroslav
M
D000208:Acute Disease; D000818:Animals; D003404:Creatinine / Q000097:blood; D016572:Cyclosporine / Q000494:pharmacology; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006084:Graft Rejection / Q000473:pathology / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D017211:Treatment Failure; D014508:Urea / Q000097:blood; D000093542:Gemcitabine
D007166:Immunosuppressive Agents; D003841:Deoxycytidine; D016572:Cyclosporine; D014508:Urea; D003404:Creatinine; D000093542:Gemcitabine