Simultaneous injection of bone marrow cells and stromal cells into bone marrow accelerates hematopoiesis in vivo.
We have previously demonstrated that stromal cells can support the proliferation and differentiation of hematopoietic cells in vitro and in vivo and that a major histocompatibility complex restriction exists between hematopoietic stem cells and stromal cells. We have also found that intra-bone marrow (IBM) injection of allogeneic bone marrow cells (BMCs) leads to more rapid reconstitution of hematopoietic cells than intravenous injection. In the present study, we examine the effect of simultaneous injection of stromal cells and BMCs into the same bone marrow on the recovery of donor hematopoietic cells and demonstrate that simultaneous IBM injection of BMCs plus stromal cells is more effective in reconstituting recipients with donor hematopoietic cells than intravenous injection of BMCs plus stromal cells or IBM injection of BMCs alone.
Zhang
Yuming
Y
First Department of Pathology, Kansai Medical University, Moriguchi, Japan.
Adachi
Yasushi
Y
Suzuki
Yasuhiro
Y
Minamino
Keizo
K
Iwasaki
Masayoshi
M
Hisha
Hiroko
H
Song
Chang Ye
CY
Kusafuka
Kimihide
K
Nakano
Keiji
K
Koike
Yasushi
Y
Wang
Jianfeng
J
Koh
Eitaku
E
Cui
Yunze
Y
Li
Chunfu
C
Ikehara
Susumu
S
D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D004730:Endothelium, Vascular / Q000166:cytology*; D005434:Flow Cytometry; D015870:Gene Expression; D006410:Hematopoiesis; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology*; D018919:Neovascularization, Physiologic; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology*
D017931:DNA Primers; D012333:RNA, Messenger