title: In vivo diffusion tensor mapping of the brain of squirrel monkey, rat, and mouse using single-shot STEAM MRI.
abstract: The purpose was to assess the potential of half Fourier diffusion-weighted single-shot STEAM MRI for diffusion tensor mapping of animal brain in vivo. A STEAM sequence with image acquisition times of about 500 ms was implemented at 2.35 T using six gradient orientations and b values of 200, 700, and 1200 s mm(-2). The use of half Fourier phase-encoding increased the signal-to-noise ratio by 45% relative to full Fourier acquisitions. Moreover, STEAM-derived maps of the relative anisotropy and main diffusion direction were completely free of susceptibility-induced signal losses and geometric distortions. Within measuring times of 3 h, the achieved resolution varied from 600x700x1000 microm3 for squirrel monkeys to 140x280x720 microm3 for mice. While in monkeys the accessible white matter fiber connections were comparable to those reported for humans, detectable fiber structures in mice focused on the corpus callosum, anterior commissure, and hippocampal fimbria. In conclusion diffusion-weighted single-shot STEAM MRI allows for in vivo diffusion tensor mapping of the brain of squirrel monkeys, rats, and mice without motion artifacts and susceptibility distortions.
authors.affiliation: Biomedizinische NMR Forschungs GmbH am Max Planck Institut für biophysikalische Chemie, 37070, Göttingen, Germany. sboreti@gwdg.de
authors.forename: S
authors.initials: S
authors.lastname: Boretius
authors.forename: O
authors.initials: O
authors.lastname: Natt
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: R
authors.initials: R
authors.lastname: Tammer
authors.forename: L
authors.initials: L
authors.lastname: Ehrenreich
authors.forename: J
authors.initials: J
authors.lastname: Frahm
authors.forename: T
authors.initials: T
authors.lastname: Michaelis
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051379:Mice; D051381:Rats; D015203:Reproducibility of Results; D012453:Saimiri; D012680:Sensitivity and Specificity; D013045:Species Specificity