Characterization of natural peptide ligands from HLA-DP2: new insights into HLA-DP peptide-binding motifs.
Although natural peptide ligands of HLA-DR and HLA-DQ molecules have been extensively studied, information about peptides naturally bound to HLA-DP is limited. Here we describe HLA-DP2 peptide ligands corresponding to 24 different source proteins that were identified by peptide pool elution and mass spectrometry sequencing from HLA-DP2 molecules expressed on EBV-LCLs. Sequencing analysis led to the identification of both promiscuous and allele-specific peptides. Moreover, the alignment of the natural ligands for HLA-DP2 described here, combined with previous results from our group and others concerning HLA-DP2 antigen presentation and HLA-DP molecular modelling, provide a better understanding of HLA-DP2 peptide-binding motifs.
Díaz
Gema
G
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, Spain.
Cañas
Benito
B
Vazquez
Jesús
J
Nombela
César
C
Arroyo
Javier
J
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D006682:HLA-DP Antigens / Q000737:chemistry* / Q000378:metabolism; D059868:HLA-DP beta-Chains; D008024:Ligands; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C083176:HLA-DPw2 antigen; D008024:Ligands