Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.
Gene ytkD of Bacillus subtilis, a member of the Nudix hydrolase superfamily, has been cloned and expressed in Escherichia coli. The purified protein has been characterized as a nucleoside triphosphatase active on all of the canonical ribo- and deoxyribonucleoside triphosphates. Whereas all other nucleoside triphosphatase members of the superfamily release inorganic pyrophosphate and the cognate nucleoside monophosphate, YtkD hydrolyses nucleoside triphosphates in a stepwise fashion through the diphosphate to the monophosphate, releasing two molecules of inorganic orthophosphate. Contrary to a previous report, our enzymological and genetic studies indicate that ytkD is not an orthologue of E. coli mutT.
Xu
Wenlian
W
Department of Biology, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA.
Jones
Candice R
CR
Dunn
Christopher A
CA
Bessman
Maurice J
MJ
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D043583:Nucleoside-Triphosphatase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D011755:Pyrophosphatases / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D000096964:Nudix Hydrolases
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D043583:Nucleoside-Triphosphatase; D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; D000096964:Nudix Hydrolases; C052211:mutT protein, E coli