title: Determination of linezolid in plasma and bronchoalveolar lavage by high-performance liquid chromatography with ultraviolet detection using a fully automated extraction method.
abstract: The aim of this study was to develop a specific and sensitive high-performance liquid chromatographic assay for the determination of linezolid in human plasma, and bronchoalveolar lavage. The sample extraction was based on a fully automated solid-phase extraction with an OASIS HLB cartridge. The method used ultraviolet detection set at a wavelength of 254 nm and a separation with a Zorbax Eclipse XDB C8 column. The assay has been found linear over the concentration range 0.02-30 microg/ml and 0.04-30 microg/ml for linezolid, respectively, in plasma and bronchoalveolar lavage. It provided good validation data for accuracy and precision (CV <4.64% and 5.08%, accuracy in the range 96.93-102.67% and 97.33-105.67%, respectively, for intra- and inter-day). The assay will be applied to determine the penetration of linezolid in human bronchoalveolar lavage during pharmacokinetic steady-state.
authors.affiliation: Clinical Pharmacokinetic Department, Victor Segalen Bordeaux 2 University, Bordeaux, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Toutain
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Boselli
authors.forename: Sarah
authors.initials: S
authors.lastname: Djabarouti
authors.forename: Bernard
authors.initials: B
authors.lastname: Allaouchiche
authors.forename: Fabien
authors.initials: F
authors.lastname: Xuereb
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Bernadou
authors.forename: Boubakar
authors.initials: B
authors.lastname: Ba
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Saux
authors.forename: Dominique
authors.initials: D
authors.lastname: Breilh
mesh_terms: D000081:Acetamides / Q000097:blood* / Q000493:pharmacokinetics; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D001331:Automation; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D000069349:Linezolid; D023303:Oxazolidinones / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D000069349:Linezolid