Selective regulation of 14-3-3eta in primary culture of cerebral cortical neurons and astrocytes during development.
The 14-3-3 proteins exist predominantly in the brain and may play regulatory roles in cellular processes of growth, differentiation, survival, and apoptosis. The biological functions, however, of the various 14-3-3 isoforms (beta, epsilon, eta, gamma, and zeta) in the brain remain unclear. We have reported previously upregulation of 14-3-3gamma in ischemic astrocytes. In the present study, we report selective regulation of 14-3-3eta in cultured cerebral cortical neurons and astrocytes during in vitro development. In cultured neurons, gene expression levels of 14-3-3eta increase with culture age (0-10 days). Brain-derived neurotrophic factor and neurotrophin-3 upregulate 14-3-3eta gene expression. In cultured astrocytes, 14-3-3eta is downregulated with culture age (1-5 weeks). The gene expression level of 14-3-3eta is not affected by scratch injury in astrocytes or by ischemia in neurons. These data suggest a possible role of 14-3-3eta in growth and differentiation of neurons and astrocytes, indicating an intricate mechanism governing coordinated and well-controlled developmental events in the brain to ensure normal neural functions.
Chen
Xiao Qian
XQ
Neuroscience Research Institute, Peking University, 38 Xue Yuan Road, Beijing 100083, China.
Liu
Shuang
S
Qin
Lu Ye
LY
Wang
Chen Ran
CR
Fung
Yin-Wan Wendy
YW
Yu
Albert Cheung-Hoi
AC
D048948:14-3-3 Proteins / Q000145:classification / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000494:pharmacology; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors; D014947:Wounds and Injuries / Q000503:physiopathology
D048948:14-3-3 Proteins; D019208:Brain-Derived Neurotrophic Factor; D020933:Neurotrophin 3; D020033:Protein Isoforms; D012333:RNA, Messenger; D020932:Nerve Growth Factor