Impact of CD34 subsets on engraftment kinetics in allogeneic peripheral blood stem cell transplantation.
Our objective was to evaluate, probably for the first time, the impact of CD34 subsets on engraftment kinetics in allogeneic PBSC transplantation (PBSCT). PBSC graft components were analyzed in 62 cases for the absolute count/kg of total CD34+ and the following subsets: DR- and +, CD71+/-, CD38+/-, CD33+/- and CD61+/-. Time to ANC >0.5 and >1 x 10(9)/l and platelets >20 and >50 x 10(9)/l was reported. The median value for each parameter was used to discriminate rapid from slow engraftment. Four parameters showed significant predictive power of early neutrophil engraftment, namely CD34+ /DR- (P = 0.002), CD34+/38- (P = 0.02), CD34+/CD61- (P = 0.04) and total CD34+ cell dose (P = 0.04). Four parameters showed significant predictive power of early platelet engraftment, namely CD34+/CD61+ (P = 0.02), CD34+ /CD38- and total CD34+ cell dose (P = 0.04) and CD34+ /CD71- (P = 0.05). Comparing patients who received > to those who received < the threshold dose(s), only CD34+ /CD38- lost its significance for neutrophil engraftment; and only CD34+ /CD61+ retained its significance for platelet engraftment (P = 0.03); furthermore, the former group required significantly fewer platelet transfusions (P = 0.018). We concluded that in allogeneic PBSCT, the best predictor of early neutrophil engraftment is the absolute CD34+ /DR- and for early platelet engraftment is the absolute CD34+ /CD61+ cell dose.
Kamel
A M
AM
NCI, Cairo University, Fom El-Khalig, Cairo, Egypt. amkamel@hotmail.com
El-Sharkawy
N
N
Mahmoud
H K
HK
Khalaf
M-R
MR
El Haddad
A
A
Fahmy
O
O
El Fattah
R Abd
RA
Sayed
D
D
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D001792:Blood Platelets / Q000502:physiology; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D005260:Female; D006085:Graft Survival; D006684:HLA-DR Antigens; D006801:Humans; D016130:Immunophenotyping; D039661:Integrin beta3; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D036102:Peripheral Blood Stem Cell Transplantation; D011237:Predictive Value of Tests; D012372:ROC Curve; D014184:Transplantation, Homologous
D015703:Antigens, CD; D018952:Antigens, CD34; D006684:HLA-DR Antigens; D039661:Integrin beta3