Gas chromatographic-mass spectrometric identification of 16alpha-hydroxy-dehydroepiandrosterone in human seminal plasma.
16alpha-hydroxydehydroepiandrosterone was for the first time detected in human seminal plasma by using a combination of extraction, solvent partition, HPLC separation and final quantification by GC-MS as pentafluorobenzyloxime trimethylsilyl ether derivative in SIM mode. Three effective mass fragments m/z 266, 356 and 446 were followed. The concentration of 16alpha-hydroxydehydroepiandrosterone amounted in average 1.06 nmol/l, being lower but close to- the product of concurrent DHEA metabolism, 7-hydroxydehydroepiandrosterone isomers, found recently in seminal plasma.
Hert
Jakub
J
Institute of Endocrinology, Národní 8, 116 94 Praha 1, Czech Republic.
Hill
Martin
M
Hampl
Richard
R
D002851:Chromatography, High Pressure Liquid; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008956:Models, Chemical; D020033:Protein Isoforms; D012661:Semen / Q000378:metabolism*
D020033:Protein Isoforms; C011277:16-hydroxydehydroepiandrosterone; D003687:Dehydroepiandrosterone