title: Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4.
abstract: The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.
authors.affiliation: Department of Virology 1, National Institute of Infectious Disease, 1-23-1 Toyama, Shinjyuku-ku, Tokyo 162-8640, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Ito
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Yamada
authors.forename: Reiko
authors.initials: R
authors.lastname: Nerome
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tajima
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
mesh_terms: D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase
chemical_list: D005456:Fluorescent Dyes; D012367:RNA, Viral; D019914:Taq Polymerase