[Production of gamma-interferon by blood mononuclear cells in patients with various types of a tuberculous process].
To elucidate the diagnostic value of gamma-interferon (gamma-IFN) in the human body, the gamma-IFN production that was spontaneous and induced by BCG Mycobacterium bovis in the supernatants of short-term cultures of peripheral mononuclear cell from 31 patients with active tuberculosis was studied. The sandwich method of enzyme immunoassay was used, by employing two monoclonal antibodies in the avidin-biotin system. The studies have established the low spontaneous production of gamma-IFN in patients with the severe progressive course of a tuberculous process (22.5 +/- 2.5 pg/ml in Group 1 versus 41.5 +/- 8.6 pg/ml in patients with the favorable course of a tuberculous process (Group 2) and 34.0 +/- 3.5 pg/ml in healthy individuals (Group 3); p(1-2) < 0.05 and p(1-3) < 0.05)). Moreover, there was a reduction in the induced production of gamma-IFH in response to BGC (37.7 +/- 33.4 pg/ml in Group 1 versus 214.9 +/- 81.9 pg/ml in Group 2 and 206.0 +/- 31.1 pg/ml in healthy individuals; p < 0.05 and p(1-3) < 0.001), which is of diagnostic and prognostic values.
Salina
T Iu
TIu
Morozova
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TI
D000328:Adult; D000368:Aged; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008875:Middle Aged; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000378:metabolism / Q000382:microbiology
D007371:Interferon-gamma