title: Production of an antiproliferative furanoheliangolide by Lychnophora ericoides cell culture.
abstract: This work reports for the first time the production a furanoheliangolide (goyazensolide) by plant cell culture. Monitoring of the goyazensolide metabolism revealed that the maximum production occurred during the lag phase of the Lychnophora ericoides callus culture. The antiproliferative activity of obtained goyazensolide was evaluated against seven cancer cell lines using MTT assay. The results revealed a potent cytotoxic activity for the furaheliangolide with IC50 values in the range of 0.06 microg/ml for CEM leukemia cells to 0.75 microg/ml for B16 melanome cells.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão, Preto-SP, Brazil.
authors.forename: Pierre Alexandre
authors.initials: PA
authors.lastname: dos Santos
authors.forename: Maria Fernanda Castro
authors.initials: MF
authors.lastname: Amarante
authors.forename: Ana Maria Soares
authors.initials: AM
authors.lastname: Pereira
authors.forename: Bianca
authors.initials: B
authors.lastname: Bertoni
authors.forename: Suzelei Castro
authors.initials: SC
authors.lastname: França
authors.forename: Cláudia
authors.initials: C
authors.lastname: Pessoa
authors.forename: Manoel Odorico
authors.initials: MO
authors.lastname: de Moraes
authors.forename: Letícia Veras
authors.initials: LV
authors.lastname: Costa-Lotufo
authors.forename: Marcio Roberto Pinho
authors.initials: MR
authors.lastname: Pereira
authors.forename: Norberto Peporine
authors.initials: NP
authors.lastname: Lopes
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000737:chemistry; D019659:Asteraceae / Q000737:chemistry*; D001952:Bridged-Ring Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005047:Etoposide / Q000494:pharmacology; D005663:Furans / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D054830:Sesterterpenes; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013256:Steroids / Q000737:chemistry
chemical_list: D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D001952:Bridged-Ring Compounds; D005663:Furans; D054830:Sesterterpenes; D013256:Steroids; C499803:furanoheliangolide; D005047:Etoposide; D004317:Doxorubicin