title: In vitro cultured islet-derived progenitor cells of human origin express human albumin in severe combined immunodeficiency mouse liver in vivo.
abstract: Studies in rodents suggest the presence of a hepatopancreatic stem cell in adult pancreas that may give rise to liver cells in vivo. The aim of the present study was to determine the ability of human islet-derived cells to adopt a hepatic phenotype in vivo. Cultured human islet-derived progenitor cells that did not express albumin in vitro were stained with the red fluorescent dye PKH26 and injected into the liver of severe combined immunodeficiency mice. After 3 or 12 weeks, red fluorescent cells were detected in 11 of 15 livers and were mostly single cells that were well integrated into the liver tissue. Human albumin was found in 8 of 11 animals by immunohistochemistry, and human albumin mRNA was detected in 4 of 10 host livers. The mechanism underlying this phenomenon seems to be transdifferentiation, because human and mouse albumin were found to be expressed in distinct cells in the host liver.
authors.affiliation: II. Medical Department, University of Mainz, Mainz, Germany. marcm@giftinfo.uni-mainz.de
authors.forename: Marc-Alexander
authors.initials: MA
authors.lastname: von Mach
authors.forename: Jan Georg
authors.initials: JG
authors.lastname: Hengstler
authors.forename: Marc
authors.initials: M
authors.lastname: Brulport
authors.forename: Michael
authors.initials: M
authors.lastname: Eberhardt
authors.forename: Wiebke
authors.initials: W
authors.lastname: Schormann
authors.forename: Matthias
authors.initials: M
authors.lastname: Hermes
authors.forename: Dirk
authors.initials: D
authors.lastname: Prawitt
authors.forename: Bernhard
authors.initials: B
authors.lastname: Zabel
authors.forename: Jens
authors.initials: J
authors.lastname: Grosche
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.forename: Beat
authors.initials: B
authors.lastname: Müller
authors.forename: Ludwig Sacha
authors.initials: LS
authors.lastname: Weilemann
authors.forename: Henryk
authors.initials: H
authors.lastname: Zulewski
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002454:Cell Differentiation; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D002871:Chromosome Banding; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000166:cytology*; D007621:Karyotyping; D008099:Liver / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals / Q000494:pharmacology; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors; D014183:Transplantation, Heterologous
chemical_list: D000418:Albumins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26; D012333:RNA, Messenger