Experimental analysis of antimicrobial action of dicyclomine hydrochloride.
Dicyclomine hydrochloride is an antispasmodic agent. The MIC of dicyclomine against standard strains of Gram positive and Gram negative bacteria were performed by NCCLS broth dilution technique. These drugs showed a rapid killing action on Gram positive bacteria, Staphylococcus aureus NCTC 6571, 8530 and several other reference strains. The killing effect against Gram negative bacteria, Shigella boydii 8 NCTC 254/66 and Salmonella typhimurium NCTC 74 showed that the drug was bacteriostatic with respect to these strains. High rate of killing was achieved for most strains of Gram positive bacteria within 2 h. When administered to Swiss strain of white mice at doses of 30 and 60 microg/g of mouse, the drug could significantly protect the animals challenged with 50 MLD of Salmonella typhimurium NCTC 74. According to chi2 test, the in vivo data were highly significant (p<0.001). Since dicyclomine showed a remarkable inhibitory action against several pathogenic bacteria, in the course of time, it may be developed as a potent antimicrobial agent for many bacterial infections.
Karaka
Prithviraj
P
Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Kumar
Kuppusamy Asok
KA
Basu
Laxmi Rani
LR
Dasgupta
Asish
A
Ray
Raja
R
Dastidar
Sujata G
SG
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004025:Dicyclomine / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D004025:Dicyclomine