title: LC-MS/MS analysis of peptides with methanol as organic modifier: improved limits of detection.
abstract: With the advent of soft ionization methods such as MALDI and ESI, mass spectrometry has become the most important technique for the analysis of proteins and peptides. ESI-MS is often preceded by separation of the peptide sample by reversed-phase liquid chromatography (LC). Acetonitrile (ACN) is the most commonly employed organic solvent in LC-ESI-MS analysis of peptides. In this report, we demonstrate that the use of methanol (MeOH) as the organic modifier improves the detection limits for analysis of peptide mixtures such as those found in tryptic digests of proteins. A nanoLC-ESI-quadrupole ion trap instrument (LCQ Deca, ThermoFinnigan) was used to analyze peptide standards, protein digests of known concentrations, and tryptic digests of 2-DGE-separated proteins. MeOH displayed excellent chromatographic performance (separation and sensitivity), and shorter gradient times were possible for chromatographic separation with MeOH versus ACN. Sensitivity levels of a few hundred attomoles were achieved with MeOH; those levels could not be achieved with ACN. In addition, MeOH-based nanoLC-MS/MS yielded superior results for the analysis of digests of 2-DGE-separated proteins. For the 14 protein spots analyzed, the success rate of protein identification with MeOH-based nanoLC-ESI-MS/MS was 100%, with multiple proteins identified in several of the spots. In contrast, ACN-based procedure failed to identify any proteins in 21% of the spots and overall identified 33% fewer proteins than the MeOH-based procedure. In summary, higher sensitivity and shorter gradient times make MeOH an excellent organic modifier for the use in nanoLC-ESI-MS/MS analysis of peptides.
authors.affiliation: The Charles B. Stout Neuroscience Mass Spectrometry Laboratory and the Department of Neurology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA. fgiorgianni@utmem.edu
authors.forename: Francesco
authors.initials: F
authors.lastname: Giorgianni
authors.forename: Achille
authors.initials: A
authors.lastname: Cappiello
authors.forename: Sarka
authors.initials: S
authors.lastname: Beranova-Giorgianni
authors.forename: Pierangela
authors.initials: P
authors.lastname: Palma
authors.forename: Helga
authors.initials: H
authors.lastname: Trufelli
authors.forename: Dominic M
authors.initials: DM
authors.lastname: Desiderio
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D000432:Methanol / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D010455:Peptides; D000432:Methanol