[Effects of hypertonic saline on erythrocyte adherence function and bacterial infection of hemorrhagic shock rabbits].
OBJECTIVE
To explore the effect of hypertonic saline on the erythrocyte adherence function and bacterial infection of hemorrhagic shock rabbits.


METHODS
60 Japanese rabbits were randomly divided into 6 groups, 10 for each group. Artery catheterization and heparin were given to the rabbits in group 1 (sham shock group). Hemorrhagic shock model was set up by bleeding resulting from carotid artery catheter in group 2 (normal saline group )and group 3 (hypertonic saline group). 30 minutes after shock, the rabbits in group 1 and group 2 were treated with normal saline and balanced salt solution containing 1 x 10(9)/kg E.coli, respectively. And the rabbits in group 3 were treated with 75 g/L NaCl solution and balanced salt solution containing 1 x 10(9)/kg E.coli. Then the survival rates of the rabbits in group 1-3 were observed. Rabbits in group 4-6 were same treatment as received, group 1-3, respectively, except that there was no E.coli in balanced salt solution. The erythrocyte immune adherence function of rabbits in group 4-6 were detected 5 hours after shock by RBC-C3bR and RBC-IC rosette forming assays.


RESULTS
The survival rate of rabbits in hypertonic saline group was significantly higher than that in normal saline group. The RBC-C3bR rosette forming rate of the normal saline treated rabbits were pronouncedly decreased, while RBC-IC rosette forming rate was notably elevated, as compared with those of either sham shock group or hypertonic saline group(P<0.01). Hypertonic saline markedly increased RBC C3bR rosette forming rate.


CONCLUSIONS
The above findings suggest that hypertonic salt solution can remarkably improve the depressed erythrocyte immune adherence function and enhance the rabbit's resistance to E.coli challenge after hemorrhagic shock.
Li
Han-chen
HC
Department of General Surgery, The 2nd Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. hanchenl@hotmail.com
Qin
Zhao-yin
ZY
Ji
Zong-zheng
ZZ
Li
Yi-ming
YM
Liu
Xian-zhen
XZ
Wang
Xi-qing
XQ
Jin
Shao-qi
SQ
D000818:Animals; D004912:Erythrocytes / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control*; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D017463:Receptors, Complement 3b / Q000276:immunology* / Q000378:metabolism; D012397:Rosette Formation; D012462:Saline Solution, Hypertonic / Q000494:pharmacology / Q000627:therapeutic use*; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000382:microbiology
D017463:Receptors, Complement 3b; D012462:Saline Solution, Hypertonic