The use of multiparametric monitoring during islet cell isolation and culture: a potential tool for in-process corrections of critical physiological factors.
Variables such as pH, pCO2, and PO2 have been established in the literature as critical factors that could affect the outcome of the islet cell processing and, therefore, the quality of the cells that could be transplanted. This report describes a highly accurate continuous multiparametric monitoring system and its evaluation for continuous monitoring of physiological variables during critical steps of the islet isolation procedure as well as during in vitro culture of the insulin-producing cells. Close monitoring of these variables could be of assistance to improve the outcome of islet cell processing, allowing to identify as soon as possible problems that could be corrected during the procedure, as well as during in vitro preservation, or shipment to remote sites.
Fraker
Chris
C
Diabetes Research Institute, Cell Transplant Center, University of Miami School of Medicine, Miami, FL 33136, USA.
Montelongo
Jorge
J
Szust
Joel
J
Khan
Aisha
A
Ricordi
Camillo
C
D002138:Calibration; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D017690:Cell Transplantation; D003470:Culture Media / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008991:Monitoring, Physiologic / Q000379:methods; D010100:Oxygen / Q000378:metabolism; D010179:Pancreas / Q000166:cytology; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D007328:Insulin; D002245:Carbon Dioxide; D010100:Oxygen