A simple imaging method for biomass determination.
An inexpensive and fast method based on images taken during growth of bacterial cells on multi-well plates was developed for biomass quantification. A correlation of 85% between the results obtained by image analysis and optical density measurements was obtained. This simple method allows the assessment of growth with highly aggregated cell cultures and the rapid screening of a large number of carbon sources.
de Carvalho
Carla C C R
CC
Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. ccarvalho@ist.utl.pt
Marques
Marco P C
MP
Fernandes
Pedro
P
da Fonseca
M Manuela R
MM
D000438:Alcohols / Q000378:metabolism; D018533:Biomass; D006838:Hydrocarbons / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D010781:Photography; D012240:Rhodococcus / Q000254:growth & development* / Q000378:metabolism
D000438:Alcohols; D006838:Hydrocarbons