[Genetic analysis of 17 biallelic markers on Y chromosome in 3 Chinese ethnic group populations].
OBJECTIVE
To study the genetic polymorphism of Y chromosome in different Chinese ethnic group populations.


METHODS
Genotypes of 17 biallelic markers located in the nonrecombining portion of the Y chromosome in 76 men from 3 Chinese ethnic group populations (Han in Shandong, Bai in Yunnan, and Tu in Qinghai) were examined with polymerase chain reaction (PCR) and allelic-specific PCR (ASPCR). Their haplotypes made of these 17 binary markers were constructed. The principle component (PC) analysis was conducted based on the haplotype frequency distribution among these 3 and other 15 published Chinese ethnic group populations.


RESULTS
The diversities of M50, M110, M103, M88, M3, and M7 were not found in these 3 populations. The frequencies of YAP+ were 23.8%, 6.7%, and 4% respectively in Tu, Bai, and Shandong Han. Eleven haplotypes were found in 3 populations--7 haplotypes (H1, H3, H5, H6, H8, H9, and H11) in Shandong Han (Han.SD), 8 haplotypes (H1, H2, H3, H5, H6, H8, H11, and H16) in Tu, and 9 haplotypes (H1, H3, H4, H5, H6, H8, H9, H11, and H13) in Bai. The predominant haplotypes were H1, H3, H5, H6, H8, and H11. According to PC analysis, Bai was close to Northern Han; Shandong Han, Southern Han (Han.S), Bai and Yunnan Tibetan clustered together; and Tu was close to Yi, Hui and Manchurian.


CONCLUSIONS
Shandong Han may have had genetic exchanges with southern populations in China. It has been confirmed that some gene components of Han had flowed into Bai's gene pool. Gene flowed from Central Asia had impacted Chinese western populations.
Yu
Jian-kun
JK
Department of Medical Genetics, Institute of Medical Biology, CAMS and PUMC, Kunming 650118, China.
Sun
Hao
H
Shi
Lei
L
Qian
Ya-ping
YP
Shi
Li
L
Huang
Xiao-qin
XQ
Chu
Jia-you
JY
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics*; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D005819:Genetic Markers