Activation of human coagulation system by liver-derived clotting factors of Banna minipig inbred line.
The liver synthesizes most of the coagulation factors that play a major role in arresting hemorrhage. Matching hepatic coagulation factors is an important premise in successful xenotransplantation. As a unique inbred pig, the Banna minipig inbred (BMI) animals have a huge potential value for pig-to-human xenotransplantation, due to its clear genetic background and tiny interindividual differences. Whether the coagulation factors synthesized by porcine liver can trigger human clotting pathways has not been reported. This study focused on the activities of BMI coagulant factors synthesized exclusively by the liver to activate human clotting pathways. In these experiments we prepared coagulant factors II, V, VII, X, and XII synthesized exclusively by liver from BMI and humans. The factors were used in common correction tests, added to the corresponding factor-deficient human plasma to determine prothrombin times or activated partial thromboplastin time, thereby calculating BMI and human coagulant factor activities. BMI clotting factors XII, VII, and X triggered human intrinsic, extrinsic, and common pathways, respectively. BMI clotting factors II, V, VII, X, and XII activities were 3.2-, 3.7-, 4.7-, 2.9-, and 4.5-fold as potent as those from humans.
Zhang
L
L
Lab of Transplant Engineering and Immunology, West China Hospital, Sichuan University, People's Republic of China.
Li
Y
Y
Liu
J
J
Zeng
Y
Y
Zeng
R
R
Cheng
J
J
D000328:Adult; D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001779:Blood Coagulation Factors / Q000502:physiology*; D006801:Humans; D007178:Inbreeding; D008099:Liver / Q000502:physiology*; D008297:Male; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature
D001779:Blood Coagulation Factors