Prothymosin alpha associates with the oncoprotein SET and is involved in chromatin decondensation.
Prothymosin alpha (ProTalpha) is a histone H1-binding protein that interacts with the transcription coactivator CREB-binding protein and potentiates transcription. Based on coimmunoprecipitation and mammalian two-hybrid assays, we show here that ProTalpha forms a complex with the oncoprotein SET. ProTalpha efficiently decondenses human sperm chromatin, while overexpression of GFP-ProTalpha in mammalian cells results in global chromatin decondensation. These results indicate that decondensation of compacted chromatin fibers is an important step in the mechanism of ProTalpha function.
Karetsou
Zoe
Z
Laboratory of Biological Chemistry, Medical School, University of Ioannina, 451 10 Ioannina, Greece.
Martic
Goran
G
Tavoulari
Sotiria
S
Christoforidis
Savvas
S
Wilm
Matthias
M
Gruss
Claudia
C
Papamarcaki
Thomais
T
D000595:Amino Acid Sequence; D015153:Blotting, Western; D050882:CREB-Binding Protein; D002457:Cell Extracts; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D056488:Histone Chaperones; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D020539:Sequence Analysis, Protein; D016622:Silver Staining; D013094:Spermatozoa / Q000378:metabolism; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques
D002457:Cell Extracts; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D009687:Nuclear Proteins; D011498:Protein Precursors; D011506:Proteins; D011994:Recombinant Proteins; C513473:SET protein, human; D015534:Trans-Activators; D014157:Transcription Factors; C040782:prothymosin alpha; D049452:Green Fluorescent Proteins; D013947:Thymosin; D008156:Luciferases; D050882:CREB-Binding Protein; C494363:CREBBP protein, human