[Recombinant human bone morphogenetic protein-2 transform NIH3T3 fibroblast to osteo-like cells].
OBJECTIVE
To examine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on NIH3T3 cells.


METHODS
The NIH3T3 cells were cultured in DMEM with 50 microg/L rhBMP-2. The proliferation ability, alkaline phosphatase (ALP) activity and osteocalcin (OC) quantity of NIH3T3 cells were investigated.


RESULTS
The cells showed decreased proliferation ability but enhanced ALP activity and OC quantity.


CONCLUSIONS
50 microg/L rhBMP-2 can transform NIH3T3 fibroblasts to osteo-like cells.
Wang
Jun-lin
JL
Dept. of Oral Histology and Pathology, The Fourth Military Medical University, Xi'an 710032, China.
Liu
Yuan
Y
Jin
Yan
Y
Wang
Xin-wen
XW
Li
Yuan
Y
D000469:Alkaline Phosphatase / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D004247:DNA / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D041681:NIH 3T3 Cells; D015675:Osteocalcin / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D015675:Osteocalcin; D004247:DNA; D000469:Alkaline Phosphatase