Effects of phenylbutazone, indomethacin, prostaglandin E2, butyrate, and glutamine on restitution of oxidant-injured right dorsal colon of horses in vitro.
OBJECTIVE
To study the effects of phenylbutazone, indomethacin, prostaglandin E2 (PGE2), glutamine, and butyrate on restitution of oxidant-injured right dorsal colon of horses in vitro.


METHODS
Right dorsal colon from 9 adult horses euthanatized for reasons other than gastrointestinal tract disease.


METHODS
Mucosal segments from the right dorsal colon were injured via exposure to HOCl and incubated in Ussing chambers in solutions containing phenylbutazone, indomethacin, indomethacin and PGE2, glutamine, and butyrate. Transepithelial resistance and mucosal permeability to mannitol were measured, and all mucosal segments were examined histologically.


RESULTS
The HOCl-injured mucosa had lower resistance and higher permeability to mannitol, compared with control tissue. Histologic changes were also evident. Resistance of HOCl-injured mucosa recovered partially during the incubation period, and glutamine improved recovery. Phenylbutazone and indomethacin increased resistance, but these increases were not significant. Butyrate and PGE2 had no effects, compared with nontreated HOCl-injured tissues. Mucosal permeability to mannitol was lower in glutamine-treated tissue, compared with nontreated tissue. Histologic changes reflected the resistance and permeability changes.


CONCLUSIONS
According to our findings, phenylbutazone and indomethacin do not seem to interfere with restitution of oxidant-injured mucosa of equine colon in vitro, and glutamine could facilitate mucosal restitution.
Rötting
Anna K
AK
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
Freeman
David E
DE
Constable
Peter D
PD
Eurell
Jo Ann C
JA
Wallig
Matthew A
MA
D000704:Analysis of Variance; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001837:Body Weights and Measures; D002087:Butyrates / Q000494:pharmacology; D003106:Colon; D015232:Dinoprostone / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D006652:Histological Techniques; D006736:Horses / Q000378:metabolism*; D006997:Hypochlorous Acid / Q000633:toxicity; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000293:injuries*; D008353:Mannitol / Q000493:pharmacokinetics*; D010539:Permeability; D010653:Phenylbutazone / Q000494:pharmacology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002087:Butyrates; D005973:Glutamine; D008353:Mannitol; D006997:Hypochlorous Acid; D010653:Phenylbutazone; D015232:Dinoprostone; D007213:Indomethacin