Comparison of oxidative damage in Malaysian end-stage renal disease patients with or without non-insulin-dependent diabetes mellitus.
BACKGROUND
Comparisons of oxidative indices and total antioxidant status between end-stage renal disease (ESRD) patients with or without diabetes is scant, especially in the Asian population.


METHODS
The assays were carried out according to known established protocols.


RESULTS
The present study showed that ESRD patients with or without non-insulin-dependent diabetes mellitus (NIDDM) did not have any significant differences in antioxidant enzyme activities, advanced glycated end products (AGE), advanced oxidized protein products (AOPP) and ferric reducing ability of plasma (FRAP), indicating that hyperglycemia does not exacerbate oxidative damage in ESRD. The regulation of catalase and glutathione peroxidase is also altered in ESRD. Elevated FRAP was observed in both ESRD groups (with and without NIDDM). The dialysis process did not alter the antioxidant enzyme activities but decreased AGEs and FRAP and increased AOPP levels.


CONCLUSIONS
Oxidative stress is present in ESRD but this is not significantly exacerbated by hyperglycemia. The contribution of components in the pathology of renal failure towards oxidative stress exceeds that of hyperglycemia.
Kuppusamy
U R
UR
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. umah@um.edu.my
Indran
M
M
Ahmad
Tsara
T
Wong
S W
SW
Tan
S Y
SY
Mahmood
A A
AA
D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology*; D005290:Ferric Compounds / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications / Q000473:pathology*; D008296:Malaysia; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D006435:Renal Dialysis
D000975:Antioxidants; D005290:Ferric Compounds; D017127:Glycation End Products, Advanced; D002374:Catalase; D005979:Glutathione Peroxidase