Simple and rapid procedure suitable for quantitative isolation of low and high molecular weight extracellular nucleic acids.
The procedure based on binding of nucleic acids with glass surface in presence of chaotropic salts was adapted for efficient isolation of 100-10000 b.p. DNA fragments and 50-10,000 b. RNA fragments. The method provide 90% and 85% efficacy of isolation of 100 b.p. DNA and 100 b. RNA fragments respectively. High molecular weight nucleic acids are isolated with 98% efficacy. Isolated nucleic acids are free from contaminations, influencing nucleic acids modifying enzymes and fluorochromes. The method is rapid, simple and cost-effective.
Tamkovich
Svetlana N
SN
Institute of Bioorganic Chemistry of Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Laktionov
Pavel P
PP
Rykova
Elena Yu
EY
Vlassov
Valentin V
VV
D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*
D009696:Nucleic Acids