title: Biallelic expression of the Gsalpha gene in human bone and adipose tissue.
abstract: Mutations of the Gsalpha gene inherited from the mother lead to pseudohypoparathyroidism (PHP) type Ia (PHP Ia), in which Albright's hereditary osteodistrophy is associated to resistance to the action of different hormones, whereas the same mutations inherited from the father lead to isolated Albright's hereditary osteodistrophy [pseudo-PHP (PPHP)]. Accordingly, it has been suggested that Gsalpha is under tissue-specific imprinting control, and recent studies provided evidence for a predominant maternal origin of Gsalpha transcripts in different endocrine organs involved in the PHP Ia phenotype. To establish whether Gsalpha is imprinted also in tissues that are site of alteration both in PHP Ia and PPHP, we selected 20 bone and 10 adipose tissue samples, which were heterozygous for a known polymorphism in exon 5. Expression from both parental alleles was evaluated by RT-PCR and enzymatic digestion of the resulting fragments. By this approach, the great majority of the samples analyzed showed an equal expression of the two alleles. Our results provide evidence for the absence of Gsalpha imprinting in human bone and fat and suggest that the clinical finding of osteodystrophy and obesity in PHP Ia and PPHP patients despite the presence of a normal Gsalpha allele is likely due to Gsalpha haploinsufficiency in these tissues.
authors.affiliation: Institute of Endocrine Sciences, University of Milano, Ospedale Maggiore Istituto di Ricovero e Cura a Carattere Scientifico, 20122 Milan, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mantovani
authors.forename: Sara
authors.initials: S
authors.lastname: Bondioni
authors.forename: Marco
authors.initials: M
authors.lastname: Locatelli
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pedroni
authors.forename: Andrea G
authors.initials: AG
authors.lastname: Lania
authors.forename: Emanuele
authors.initials: E
authors.lastname: Ferrante
authors.forename: Marcello
authors.initials: M
authors.lastname: Filopanti
authors.forename: Paolo
authors.initials: P
authors.lastname: Beck-Peccoz
authors.forename: Anna
authors.initials: A
authors.lastname: Spada
mesh_terms: D000273:Adipose Tissue / Q000196:embryology / Q000378:metabolism*; D000483:Alleles; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D005333:Fetus / Q000378:metabolism; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006579:Heterozygote; D006801:Humans; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019205:GTP-Binding Protein alpha Subunits, Gs