Triterpenoid from Tiarella polyphylla, regulation of type 1 procollagen and MMP-1 in ultraviolet irradiation of cultured old age human dermal fibroblasts.
Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of natural products. Ultraviolet irradiation is widely considered to be an environmental stress. Here we investigated the effect of 3,23-dihydroxy-20(29)-lupen-27-oic acid on the regulation of MMP-1 and type 1 procollagen in Ultraviolet irradiation of cultured old age human dermal fibroblasts. 3, 23-dihydroxy-20(29)-lupen-27-oic acid was isolated from Tiarella polyphylla D. Don (Saxifragaceae). Among them, 3, 23-dihydroxy-20(29)-lupen-27-oic acid induced the regulation of Type 1- procollagen and reduced the regulation of MMP-1 at the protein levels in a dose-dependent manner by ultraviolet irradiation. Taken together, our results suggest that 3, 23-dihydroxy-20(29)-lupen-27-oic acid plays an important role in the induction of Type 1-procollagen and reduction of MMP-1 by ultraviolet irradiation in old age human dermal fibroblasts.
Moon
Hyung-In
HI
Derpartment of Dermatology, Seoul National University, College of Medicine, and Clinical Research Institute, Seoul National University Hosipital, Seoul 110-744, Korea.
Lee
Joongku
J
Zee
Ok Pyo
OP
Chung
Jin Ho
JH
D000368:Aged / Q000502:physiology*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D007118:Immunoassay; D009682:Magnetic Resonance Spectroscopy; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D031995:Saxifragaceae / Q000737:chemistry*; D012867:Skin / Q000166:cytology / Q000187:drug effects; D012997:Solvents; D014315:Triterpenes / Q000494:pharmacology*; D014466:Ultraviolet Rays
D024042:Collagen Type I; D012997:Solvents; D014315:Triterpenes; D020781:Matrix Metalloproteinase 1