title: Analysis of the carbohydrate binding specificity of the mushroom Pleurotus ostreatus lectin by surface plasmon resonance.
abstract: The sugar binding specificity of the mushroom Pleurotus ostreatus lectin (POL) was analyzed by surface plasmon resonance. The lectin was immobilized to a sensor chip, and asialo-bovine submaxillary mucin (asialo-BSM), one of the most potent inhibitors in the hemagglutination inhibition assay, tightly bound to the lectin. The binding specificity of various mono- or oligosaccharides to the lectin was evaluated by the coinjection method. The dissociation of asialo-BSM was promoted by injection of some haptenic saccharides. For the most part, the order of acceleration ability of the sugars to the dissociation in the coinjection experiment agreed with that of the inhibitory potency of each sugar evaluated by the hemagglutination inhibition assay. In conclusion, POL recognized a galactosyl residue, and the specificity was increased by substitution at the C-2 position of the galactosyl residue with a fucosyl or acetylamino group. This method using the coinjection method proved useful in analysis of carbohydrate-lectin binding specificity.
authors.affiliation: Science of Biological Resource, The United Graduate School of Agricultural Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakamura
authors.forename: Takehiko
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: Ryo
authors.initials: R
authors.lastname: Takanami
authors.forename: Takeomi
authors.initials: T
authors.lastname: Murata
authors.forename: Taichi
authors.initials: T
authors.lastname: Usui
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
mesh_terms: D001212:Asialoglycoproteins / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry*; D006385:Hemagglutination Inhibition Tests; D007700:Kinetics; D037102:Lectins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D009077:Mucins / Q000737:chemistry; D020076:Pleurotus / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D001212:Asialoglycoproteins; D002241:Carbohydrates; D037102:Lectins; D009077:Mucins