Neutrophils secrete MIP-1 beta after adhesion to laminin contained in basement membrane of blood vessels.
We have recently demonstrated that granulocyte colony-stimulating factor (G-CSF) stimulated the production of epithelial-cell-derived-neutrophil attractant-78 (ENA-78) by neutrophils and that ENA-78 might promote the accumulation of neutrophils that had migrated from the intravascular space into inflammatory tissues. In this study, we examined whether other chemokines could be secreted by neutrophils that had accumulated after migrating from the intravascular space into the inflammatory tissues. We demonstrated that adhesion to laminin contained in the basement membrane and Matrigel, which is an artificial basement membrane model, induced macrophage inflammatory protein-1 beta (MIP-1 beta) in neutrophils and that MIP-1 beta secreted by neutrophils induced the chemotaxis of dendritic cells. These findings suggest that neutrophils transmigrating through the basement membrane are stimulated to secrete MIP-1 beta by the basement membrane, inducing the transmigration of dendritic cells from the intravascular space into inflammatory tissues. We propose that neutrophils intervene between innate immune response and specific immune response by secreting MIP-1 beta during the transmigration through the basement membrane.
Chiba
Kouji
K
Department of Gastroenterology and Hematology, Hokkaido University Graduate School of Medicine, Japan.
Zhao
Wenli
W
Chen
Jiang
J
Wang
Jingxin
J
Cui
Hong Yan
HY
Kawakami
Hiroshi
H
Miseki
Tetsuya
T
Satoshi
Hashino
H
Tanaka
Junji
J
Asaka
Masahiro
M
Kobayashi
Masanobu
M
D001485:Basement Membrane / Q000378:metabolism*; D001808:Blood Vessels / Q000378:metabolism / Q000648:ultrastructure*; D002448:Cell Adhesion; D002478:Cells, Cultured; D054407:Chemokine CCL4; D002634:Chemotaxis, Leukocyte; D003094:Collagen; D003713:Dendritic Cells / Q000276:immunology; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D019402:Macrophage Inflammatory Proteins / Q000235:genetics / Q000378:metabolism*; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism*; D011509:Proteoglycans; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054407:Chemokine CCL4; D004338:Drug Combinations; D007797:Laminin; D019402:Macrophage Inflammatory Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; C059485:matrigel; D003094:Collagen