In vivo DNA damaging potential of sanguinarine alkaloid, isolated from argemone oil, using alkaline Comet assay in mice.
Consumption of mustard oil contaminated with argemone oil is well known to cause clinical manifestation referred to as "Epidemic Dropsy". Our prior studies have shown that argemone oil produces genotoxic effects in mice [Ansari, K.M., et al., 2004. Int. J. Cancer 112, 890]. Since, sanguinarine alkaloid is the major component of argemone oil, the in vivo DNA damaging potential of the isolated alkaloid was investigated in blood and bone marrow cells of mice using alkaline Comet assay. Swiss albino male mice were given single intraperitoneal administration of 1.35, 2.70, 5.40, 10.80 and 21.60 mg sanguinarine alkaloid/kg b wt., while controls were treated with saline in the same manner. The results revealed a dose dependent increase in DNA damage in blood and bone marrow cells following 24 h treatment of sanguinarine alkaloid. All the three parameters of Comet assay including olive tail moment (OTM), tail length and tail DNA showed significant (p<0.05) increases in blood and bone marrow cells at respective doses of 10.80 and 5.40 mg alkaloid/kg b wt. However, some of the parameters were significantly increased even at lower doses of sanguinarine alkaloid (2.70 mg/kg b wt.). The frequency of cells exhibiting greater DNA damage were found to be increased by sanguinarine alkaloid in a concentration dependent manner. These results indicate that single exposure of sanguinarine alkaloid causes DNA damage in blood and bone marrow cells of mice, which could be responsible for the genotoxicity of argemone oil.
Ansari
Kausar M
KM
Food Toxicology Division, Industrial Toxicology Research Centre, Mahatma Gandhi Marg, P.O. Box No. 80, Lucknow 226001, India.
Dhawan
Alok
A
Khanna
Subhash K
SK
Das
Mukul
M
D000470:Alkaloids / Q000633:toxicity*; D000818:Animals; D053119:Benzophenanthridines; D001854:Bone Marrow Cells / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D007546:Isoquinolines; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D010938:Plant Oils / Q000737:chemistry*; D011897:Random Allocation
D000470:Alkaloids; D053119:Benzophenanthridines; D007546:Isoquinolines; D010938:Plant Oils; C030610:argemone oil; C005705:sanguinarine