title: Theoretical investigations of prostatic acid phosphatase.
abstract: The phosphotyrosyl protein phosphatase activity of prostatic acid phosphatase (PAP) has been well established. It has also been suggested that PAP partly regulates the activity of growth factor receptors by dephosphorylating the autophosphorylysable tyrosines in them. We studied the binding of the peptides from epidermal growth factor receptor (EGFR) and its homolog (ErbB-2), corresponding to their autophosphorylation sites, to PAP using theoretical modeling and molecular dynamics (MD) simulation methods. Nine different peptides, each with a phosphotyrosine residue, were docked on human PAP. The binding energies of these peptide-PAP complexes were calculated theoretically and compared to experimentally obtained affinities. The peptide Ace--DNLpYYWD--NH2 from ErbB-2(1197-1203) showed the most favorable free energy of binding when estimated theoretically. The results demonstrate that the presence of another tyrosine residue proximate to C-terminal of autophosphorylysable Tyr enhances the binding affinity considerably. The presence of a bulky group instead prevents the binding, as is observed in case of peptide Ace--NLYpYWDQ--NH2 which failed to bind, both in theoretical calculations and experiments. Thus we demonstarted that PAP could potentially bind to EGFR and Erbb-2 and dephosphorylate them. Thus it could be involved in the regulation of the function of such receptors. In addition, complexes of a peptide from AngiotensinII and phosphotyrosine(pY) with human PAP were also modeled. The effects of different protonation states of the titratable active site residues on ligand (pY) binding have also been investigated. For a favorable binding His12 and Asp258 should be neutral, His257 should be positively charged and the phosphate group of the ligand should be in PO(4) (3-) state. Furthermore, the analysis of protein motion as observed during simulations suggests the loop-loop contact in the PAP dimer to be of importance in cooperativity.
authors.affiliation: Biocenter Oulu, University of Oulu, Department of Biochemistry, University of Oulu, Oulu, Finland.
authors.forename: Satyan
authors.initials: S
authors.lastname: Sharma
authors.forename: Päivi
authors.initials: P
authors.lastname: Pirilä
authors.forename: Helena
authors.initials: H
authors.lastname: Kaija
authors.forename: Katja
authors.initials: K
authors.lastname: Porvari
authors.forename: Pirkko
authors.initials: P
authors.lastname: Vihko
authors.forename: André H
authors.initials: AH
authors.lastname: Juffer
mesh_terms: D000135:Acid Phosphatase; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D001665:Binding Sites; D003198:Computer Simulation; D019281:Dimerization; D066246:ErbB Receptors / Q000737:chemistry / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008956:Models, Chemical; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D051381:Rats; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry; D055672:Static Electricity; D013816:Thermodynamics; D014443:Tyrosine / Q000737:chemistry
chemical_list: D000135:Acid Phosphatase; D001224:Aspartic Acid; D066246:ErbB Receptors; D006639:Histidine; D008024:Ligands; D010455:Peptides; D019000:Phosphotyrosine; D017027:Protein Tyrosine Phosphatases; D011506:Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D014443:Tyrosine; C428439:prostatic acid phosphatase