title: Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
abstract: Plasmacytoid dendritic cells (pDCs) recognize microbes, viruses in particular, and provide unique means of innate defense against them. The mechanism of pDC tissue recruitment remained enigmatic because the ligands of CXCR3, the cardinal chemokine receptor on pDCs, have failed to induce in vitro chemotaxis of pDCs in the absence of additional chemokines. In this study, we demonstrate that CXCR3 is sufficient to induce pDC migration, however, by a migratory mechanism that amalgamates the features of haptotaxis and chemorepulsion. To mediate "haptorepulsion" of pDCs, CXCR3 requires the encounter of its cognate ligands immobilized, optimally by heparan sulfate, in a form of a negative gradient. This is the first report of the absolute requirement of chemokine immobilization and presentation for its in vitro promigratory activity. The paradigmatic example of pDC haptorepulsion described here may represent a new pathophysiologically relevant migratory mechanism potentially used by other cells in response to other chemokines.
authors.affiliation: Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
authors.forename: Norbert
authors.initials: N
authors.lastname: Kohrgruber
authors.forename: Marion
authors.initials: M
authors.lastname: Gröger
authors.forename: Paul
authors.initials: P
authors.lastname: Meraner
authors.forename: Ernst
authors.initials: E
authors.lastname: Kriehuber
authors.forename: Peter
authors.initials: P
authors.lastname: Petzelbauer
authors.forename: Sabine
authors.initials: S
authors.lastname: Brandt
authors.forename: Georg
authors.initials: G
authors.lastname: Stingl
authors.forename: Antal
authors.initials: A
authors.lastname: Rot
authors.forename: Dieter
authors.initials: D
authors.lastname: Maurer
mesh_terms: D017951:Antigen Presentation; D002448:Cell Adhesion / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000276:immunology*; D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D018925:Chemokines / Q000494:pharmacology; D019743:Chemokines, CXC / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D006497:Heparan Sulfate / Q000276:immunology / Q000378:metabolism; D006562:Herpes Zoster / Q000276:immunology / Q000473:pathology; D014645:Herpesvirus 3, Human / Q000276:immunology; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D008024:Ligands; D037342:Pertussis Toxin / Q000494:pharmacology; D010766:Phosphorylation; D011485:Protein Binding / Q000276:immunology; D054400:Receptors, CCR7; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000502:physiology*; D018139:Simplexvirus / Q000276:immunology; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014443:Tyrosine / Q000378:metabolism
chemical_list: D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D018925:Chemokines; D019743:Chemokines, CXC; D006497:Heparan Sulfate; D016898:Interferon-alpha; D008024:Ligands; D037342:Pertussis Toxin; D054400:Receptors, CCR7; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine; D014443:Tyrosine; C515370:CCR7 protein, human; C515255:CXCL12 protein, human; C515187:CXCR3 protein, human