title: Enhancing the first enzymatic step in the histidine biosynthesis pathway increases the free histidine pool and nickel tolerance in Arabidopsis thaliana.
abstract: Naturally selected nickel (Ni) tolerance in Alyssum lesbiacum has been proposed to involve constitutively high levels of endogenous free histidine. Transgenic Arabidopsis thaliana expressing a Salmonella typhimurium ATP phosphoribosyl transferase enzyme (StHisG) resistant to feedback inhibition by histidine contained approximately 2-fold higher histidine concentrations than wild type plants. Under exposure to a toxic Ni concentration, biomass production in StHisG expressing lines was between 14- and 40-fold higher than in wild-type plants. This suggested that enhancing the first step in the histidine biosynthesis pathway is sufficient to increase the endogenous free histidine pool and Ni tolerance in A. thaliana.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Wycisk
authors.forename: Eugene J
authors.initials: EJ
authors.lastname: Kim
authors.forename: Julian I
authors.initials: JI
authors.lastname: Schroeder
authors.forename: Ute
authors.initials: U
authors.lastname: Krämer
mesh_terms: D001276:ATP Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D006639:Histidine / Q000096:biosynthesis*; D009532:Nickel / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified; D012989:Soil Pollutants
chemical_list: D001426:Bacterial Proteins; D012989:Soil Pollutants; D006639:Histidine; D009532:Nickel; D001276:ATP Phosphoribosyltransferase