The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase.
The pur6 gene of the puromycin biosynthetic gene (pur) cluster from Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking L-tyrosine to both 3'-amino-3'-deoxyadenosine and N6,N6-dimethyl-3'-amino-3'-deoxyadenosine with a peptide-like bond. Identical reactions were performed by cell lysates from Streptomyces lividans or Escherichia coli transformants that expressed pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and O-demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl-aminonucleoside synthetase of the puromycin biosynthetic pathway.
Angel Rubio
Miguel
M
Centro de Biología Molecular "Severo Ochoa", CSIC/UAM, Universidad Autónoma, 28049 Madrid, Spain.
Barrado
Patricia
P
Carlos Espinosa
Juan
J
Jiménez
Antonio
A
Fernández Lobato
María
M
D002627:Chemistry, Physical / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression; D005798:Genes, Bacterial; D007700:Kinetics; D015394:Molecular Structure; D005810:Multigene Family; D010957:Plasmids; D011691:Puromycin / Q000096:biosynthesis*; D011692:Puromycin Aminonucleoside / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D048170:Streptomyces lividans / Q000235:genetics
D011691:Puromycin; D011692:Puromycin Aminonucleoside