title: Phorbol ester-induced differentiation of L6 myogenic cells involves phospholipase D activation.
abstract: TPA, a potent PKC activator, inhibits myogenic differentiation and activates phospholipase D (PLD). We evaluated the involvement of PLD in the TPA effects on L6 myoblasts differentiation. TPA, at concentrations inhibiting differentiation of L6 cells, induced a strong, though transient, PLD activation. Surprisingly, at nanomolar concentration, TPA induced both myogenic differentiation and sustained activation of PLD. Differential effect of TPA can be ascribed to PKC downregulation induced by highest TPA concentrations. TPA-induced differentiation was inhibited by 1-butanol, confirming the involvement of PLD in this effect. These data suggest that prolonged elevation of PLD activity is required for myogenic differentiation.
authors.affiliation: Laboratoire de Physiopathologie des Lipides et Membranes, Institut National de la Santé et de la Recherche Médicale Unité 585, Institut National des Sciences Appliquées de Lyon, 69621 Villeurbanne, France.
authors.forename: Hiba
authors.initials: H
authors.lastname: Komati
authors.forename: Alessandra
authors.initials: A
authors.lastname: Minasi
authors.forename: Fabio
authors.initials: F
authors.lastname: Naro
authors.forename: Michel
authors.initials: M
authors.lastname: Lagarde
authors.forename: Annie-France
authors.initials: AF
authors.lastname: Prigent
authors.forename: Sergio
authors.initials: S
authors.lastname: Adamo
authors.forename: Georges
authors.initials: G
authors.lastname: Némoz
mesh_terms: D020001:1-Butanol / Q000494:pharmacology; D000199:Actins / Q000378:metabolism; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004789:Enzyme Activation; D007700:Kinetics; D032446:Myoblasts / Q000187:drug effects*; D018008:Myogenin / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology*; D010739:Phospholipase D / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000199:Actins; D002273:Carcinogens; C503803:Myog protein, rat; D018008:Myogenin; D010703:Phorbol Esters; D020001:1-Butanol; D011493:Protein Kinase C; D010739:Phospholipase D; D009218:Myosins; D013755:Tetradecanoylphorbol Acetate