title: Humoral and delayed-type hypersensitive responses against listeria monocytogenes phosphatidylinositol-specific phospholipase C in experimentally infected buffaloes.
abstract: The kinetics of antibody production against phosphatidylinositol-specific phospholipase C (PI-PLC) and the isolation pattern of Listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic L. monocytogenes. Antibodies to PI-PLC appeared by 4-8 days post infection (PI), with a peak between days 7 and 16 PI, when tested by indirect plate-ELISA. Subsequently, antibody titres in all the animals declined and became undetectable on days 26-35 PI onwards until the study concluded on day 211 PI. Dot-ELISA could detect the antibodies to PI-PLC 1-2 days earlier and at higher titres as compared to plate-ELISA. L. monocytogenes could be recovered from faeces, nasal swabs and haemocultures from days 2 to 33, days 2 to 21 and days 11 to 17 PI, respectively. Antibodies to PI-PLC were detected during the course of active infection but their titres declined sharply once animals became culturally negative. Sonicated antigen elicited the highest delayed-type hypersensitivity response, followed by PI-PLC and listeriolysin O.
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India.
authors.forename: S P
authors.initials: SP
authors.lastname: Chaudhari
authors.forename: S V S
authors.initials: SV
authors.lastname: Malik
authors.forename: S B
authors.initials: SB
authors.lastname: Barbuddhe
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology; D001831:Body Temperature; D002020:Buffaloes / Q000276:immunology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D006360:Heat-Shock Proteins / Q000276:immunology; D006460:Hemolysin Proteins; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007111:Immunity, Cellular / Q000276:immunology; D008089:Listeria monocytogenes / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification; D008088:Listeriosis / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D043265:Phosphatidylinositol Diacylglycerol-Lyase / Q000276:immunology*; D054801:Phosphoinositide Phospholipase C
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; C038705:hlyA protein, Listeria monocytogenes