Bioeffect of ultrasound on endothelial cells in vitro.
The effects of low-intensity ultrasound (US) on biological systems have been investigated extensively; however, the effects of ultrasound stimulation on endothelial cells were rarely studied. In this study, 1 MHz, pulsed 1:4, and four different spatial-average temporal-peak intensities (0.5, 1.0, 1.6, and 2W/cm2) of ultrasound were used to stimulate endothelial cells for 10 min per day. The results showed that ultrasound (intensity 1.6-2.0W/cm2) treatment after 6 days enhanced the nitric oxide (NO) and Ca2+ release from the endothelial cells but did not promote cell growth. In addition, ultrasound stimulation changed the cellular morphology and orientation, and increased extracellular matrix secretion from endothelial cells.
Hsu
Shan-hui
SH
Department of Chemical Engineering, National Chung Hsing University, Taichung 40227, Taiwan ROC. shhsu@nchu.edu.tw
Huang
Tsung-bin
TB
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000502:physiology / Q000528:radiation effects* / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D042783:Endothelial Cells / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology* / Q000528:radiation effects*; D005109:Extracellular Matrix / Q000502:physiology / Q000528:radiation effects* / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism*; D011829:Radiation Dosage; D014465:Ultrasonics; D014463:Ultrasonography
D009569:Nitric Oxide; D002118:Calcium