Proteomic analysis of salt-sensitive outer membrane proteins of Vibrio parahaemolyticus.
Vibrio parahaemolyticus, a universal marine pathogen with available genome sequences, could be used as a bacterial model to clarify the various physiological phenomena of its native and host environments. In the present study, proteomic methodologies were applied to investigate the expression pattern of outer membrane proteins (OMPs) of V. parahaemolyticus at different NaCl concentrations. OmpW, OmpV, elongation factor TU and polar flagellin were determined to be osmoregulation-sensitive OMPs, among which OmpW and OmpV were reported to vary with changed NaCl concentrations in the pattern of osmolarity regulation. Therefore, our results not only expand our knowledge on osmoregulation-related proteins, but also provide a valuable strategy for the screening of salt-sensitive proteins.
Xu
Changxin
C
Center for Proteomics, Department of Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, PR China.
Ren
Haixia
H
Wang
Sanying
S
Peng
Xuanxian
X
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D007091:Image Processing, Computer-Assisted; D009997:Osmotic Pressure; D040901:Proteomics / Q000379:methods; D012965:Sodium Chloride / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014736:Vibrio parahaemolyticus / Q000737:chemistry / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D012965:Sodium Chloride