title: Silencing of c-myc expression in tumor cells by siRNA.
abstract: Suppression of c-myc protooncogene expression in KB-3-1 cells by siRNA was investigated. The siRNA duplex targeted to the exon 3 of c-myc mRNA was prepared by in vitro transcription with T7 RNA polymerase on short dsDNA-templates. It was found that incubation of KB-3-1 cells in the presence of 75 nM siRNA results in decrease of the c-myc mRNA level down to 5% of the level in the control cells and significant decline of KB-3-1 cell proliferation rate. Using 200 nM siRNA four-fold decrease of KB-3-1 cells proliferation rate was observed and this effect was stable at least 96 h after transfection.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. pimanova@mail.ru
authors.forename: T O
authors.initials: TO
authors.lastname: Kabilova
authors.forename: E L
authors.initials: EL
authors.lastname: Chernolovskaya
authors.forename: A V
authors.initials: AV
authors.lastname: Vladimirova
authors.forename: V V
authors.initials: VV
authors.lastname: Vlassov
mesh_terms: D001483:Base Sequence; D002455:Cell Division; D045744:Cell Line, Tumor; D017931:DNA Primers; D020868:Gene Silencing / Q000502:physiology*; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D034741:RNA, Small Interfering / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering