title: Genome assembly and particle maturation of the birnavirus infectious pancreatic necrosis virus.
abstract: In this study, we have analyzed the morphogenesis of the birnavirus infectious pancreatic necrosis virus throughout the infective cycle in CHSE-214 cells by using a native agarose electrophoresis system. Two types of viral particles (designated A and B) were identified, isolated, and characterized both molecularly and biologically. Together, our results are consistent with a model of morphogenesis in which the genomic double-stranded RNA is immediately assembled, after synthesis, into a large (66-nm diameter) and uninfectious particle A, where the capsid is composed of both mature and immature viral polypeptides. Upon maturation, particles A yield particles B through the proteolytic cleavage of most of the remaining viral precursors within the capsid, the compaction of the particle (60-nm diameter), and the acquisition of infectivity. These studies will provide the foundation for further analyses of birnavirus particle assembly and RNA replication.
authors.affiliation: Laboratorio de Virología, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile. rodrigo.villanueva@ibl.fr.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Villanueva
authors.forename: José L
authors.initials: JL
authors.lastname: Galaz
authors.forename: Juan A
authors.initials: JA
authors.lastname: Valdés
authors.forename: Matilde M
authors.initials: MM
authors.lastname: Jashés
authors.forename: Ana María
authors.initials: AM
authors.lastname: Sandino
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016679:Genome, Viral; D018061:Infectious pancreatic necrosis virus / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity*; D009024:Morphogenesis; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012474:Salmon / Q000821:virology; D014771:Virion / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral