title: Subsecond chiral separations on a microchip.
abstract: Fast chiral separation of DNS-amino acids could be realized using microchip electrophoresis with fluorescence detection. For this purpose, highly sulfated cyclodextrins (HS-gamma-CD) were used as chiral selectors enabling high selectivity. Even subsecond separation of DNS-tryptophan, DNS-norleucine, DNS-phenylalanine, DNS-methionine, and DNS-aspartic acid could be achieved. Baseline separation could be accomplished within 720 ms, which is the fastest separation of enantiomers reported to date. A more complex mixture consisting of three chiral DNS-amino acids could be separated within 3.3 s utilizing a separation length of only 7 mm and an electrical field strength of 2012 V/cm.
authors.affiliation: Abteilung für Chromatographie, Max-Planck-Institut für Kohlenforschung, Mülheim an der Ruhr, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Piehl
authors.forename: Martin
authors.initials: M
authors.lastname: Ludwig
authors.forename: Detlev
authors.initials: D
authors.lastname: Belder
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification; D003505:Cyclodextrins; D003619:Dansyl Compounds; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D009904:Optical Rotation; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D003505:Cyclodextrins; D003619:Dansyl Compounds