Measurement of the carbohydrate-binding specificity of lectins by a multiplexed bead-based flow cytometric assay.
Carbohydrate binding underlies many cell recognition events. Here, we describe a multiplexed glyco-bead array method for determining the carbohydrate-binding specificities of plant lectins using a bead-based flow cytometric analysis. N-glycans including high mannose, hybrid, and complex types and O-glycans from glycoproteins were immobilized on multiplexed beads, and the specificities of 13 kinds of sugar chains were monitored within 2 h in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples and allows the reliable and simultaneous elucidation of sugar-binding properties under identical conditions.
Yamamoto
Kazuo
K
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8562 Chiba, Japan. yamamoto@k.u-tokyo.ac.jp
Ito
Seiichiro
S
Yasukawa
Fumiko
F
Konami
Yukiko
Y
Matsumoto
Naoki
N
D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006020:Glycopeptides / Q000378:metabolism*; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000032:analysis / Q000378:metabolism; D008863:Microspheres; D012680:Sensitivity and Specificity
D006020:Glycopeptides; D037102:Lectins; D037241:Mannose-Binding Lectins