Biochemical and growth regulatory activities of the HIN-200 family member and putative tumor suppressor protein, AIM2.
The human HIN-200 family member AIM2 was originally identified in a screen for suppressors of melanoma tumorigenicity following introduction of chromosome 6 into the UACC903 human melanoma cell line. Although the AIM2 protein contained many of the conserved structural motifs common to other HIN-200 proteins, the biochemical characteristics of AIM2 and the ability of overexpressed AIM2 to phenocopy the effect of introduction of chromosome 6 in the UACC903 cells had not been assessed. Herein we demonstrated that AIM2 was localised within the nucleus of transfected or interferon-treated human cells. In addition, AIM2 could homodimerise via the amino-terminal (PAAD/DAPIN) region and heterodimerise with the related IFI 16 protein. However, overexpressed AIM2 did not significantly affect the growth or survival of UACC903 cells or another human melanoma cell line. These data indicate that AIM2 has many of the biochemical and structural characteristics of HIN-200 proteins, however, its expression is not sufficient to induce a tumor-suppressor-like phenotype.
Cresswell
Kim S
KS
The Peter MacCallum Cancer Centre, St. Andrews Place, East Melbourne, 3002 Vic., Australia.
Clarke
Christopher J P
CJ
Jackson
Jacob T
JT
Darcy
Phillip K
PK
Trapani
Joseph A
JA
Johnstone
Ricky W
RW
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D019281:Dimerization; D015870:Gene Expression; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D005810:Multigene Family; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D021381:Protein Transport; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
C410167:AIM2 protein, human; D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D025521:Tumor Suppressor Proteins; D007372:Interferons