title: Expression levels of protein kinase C-alpha in non-small-cell lung cancer.
abstract: Current treatments of non-small-cell lung cancer (NSCLC) are inadequate and new therapies are being developed that target specific cellular signaling proteins associated with tumor growth. One potential target is protein kinase C (PKC)-alpha, a signaling molecule with an important role in cell regulation and proliferation. The present study examines the expression levels of PKC-alpha in NSCLC to better understand the distribution of PKC-alpha in NSCLC. We analyzed tumor specimens from an independent tumor tissue bank to determine PKC-alpha protein and messenger RNA gene expression in NSCLC. In addition, we used publicly available gene expression array data to further understand PKC-a-associated gene expression profiles in NSCLC. We found that PKC-alpha is highly expressed in < or = 20% of patients with NSCLC. We also found that PKC-alpha was preferentially expressed in adenocarcinoma compared with squamous cell carcinoma of the lung.
authors.affiliation: Oncology Product Development, Eli Lilly and Company, Indianapolis, IN 46285, USA. mlahn@lilly.com
authors.forename: Michael
authors.initials: M
authors.lastname: Lahn
authors.forename: Chen
authors.initials: C
authors.lastname: Su
authors.forename: Shuyu
authors.initials: S
authors.lastname: Li
authors.forename: Marcio
authors.initials: M
authors.lastname: Chedid
authors.forename: Kimberly R
authors.initials: KR
authors.lastname: Hanna
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: Graff
authors.forename: George E
authors.initials: GE
authors.lastname: Sandusky
authors.forename: Doreen
authors.initials: D
authors.lastname: Ma
authors.forename: Clet
authors.initials: C
authors.lastname: Niyikiza
authors.forename: Karen L
authors.initials: KL
authors.lastname: Sundell
authors.forename: William J
authors.initials: WJ
authors.lastname: John
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Giordano
authors.forename: David G
authors.initials: DG
authors.lastname: Beer
authors.forename: Blake M
authors.initials: BM
authors.lastname: Paterson
authors.forename: Eric Wen
authors.initials: EW
authors.lastname: Su
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Bumol
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011493:Protein Kinase C / Q000235:genetics*; D051571:Protein Kinase C-alpha; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha