Neosynthesis of cardiolipin in Rhodobacter sphaeroides under osmotic stress.
The phospholipid composition of Rhodobacter sphaeroides cells resuspended in various hypertonic solutions has been examined by thin-layer chromatography and ESI mass spectrometry. R. sphaeroides responds to hyperosmotic stress by increasing the amount of cardiolipin in the membranes; this phenomenon occurs in spheroplasts also. Cardiolipin increases quickly and continuously during the time when the cells are resuspended in hypertonic medium. The optimum of stimulation of the neosynthesis of cardiolipin during osmotic stress was found to be at external 1 osm. ESI-MS analyses allowed the identification of two different cardiolipins in R. sphaeroides: the tetravaccenylcardiolipin ([M - H](-), m/z 1456.9) and the trivaccenylmonopalmitoylcardiolipin ([M - H](-), m/z 1430.0).
Catucci
Lucia
L
Dipartimento di Chimica, Università degli Studi di Bari, Bari, Italy.
Depalo
Nicoletta
N
Lattanzio
Veronica M T
VM
Agostiano
Angela
A
Corcelli
Angela
A
D002308:Cardiolipins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D003720:Densitometry; D006982:Hypertonic Solutions / Q000494:pharmacology; D007700:Kinetics; D008563:Membrane Lipids / Q000378:metabolism; D009997:Osmotic Pressure; D012242:Rhodobacter sphaeroides / Q000254:growth & development / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013104:Spheroplasts / Q000737:chemistry*
D002308:Cardiolipins; D006982:Hypertonic Solutions; D008563:Membrane Lipids