[Experimental study on the antiviral mechanism of Ceratostigma willmattianum against herpes simplex virus type 1 in vitro].
OBJECTIVE
To study the antiviral effect and mechanisms of the liquid extract from Ceratostigma willmattianum against herpes simplex virus type 1 (HSV-1) in vitro.


METHODS
C. willmattianum in various concentration was applied to different steps of HSV-1 replication cycle. 50% Tissue culture infective dose (TCID50), cytopathic effect (CPE), MTT staining method, dot blotting and Northern blotting analysis were used to estimate index of antiviral activity.


RESULTS
50% Toxic concentration (TC50) was 1077 mg x L(-1), IC50 29.46 mg x L(-1) and therapeutic index (TI) 36.56 in C. willmattianum. TC50 330 mg x L(-1), 50% Inhibiting concentration (IC50) 9.12 mg x L(-1) and TI 36.18 in ACV by MTT staining method. The liquid extract from C. willmattianum had remarkable effect on inhibiting HSV-1 in vitro. Ceratostigma could interfere absorption of HSV-1 to Vero cells to prevent HSV-1 infectivity, inhibit HSV-1 gD DNA replication and HSV-1 gD mRNA expression.


CONCLUSIONS
C. willmattianum possesses strong anti-HSV-1 activity in vitro. The antiviral mechanisms are related to inhibiting virus absorption, HSV-1 gD gene replication and HSV-1 gD gene transcription.
Chen
Tian
T
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041,China.
Jia
Wen-xiang
WX
Yang
Fa-long
FL
Xie
Yi
Y
Yang
Wei-qing
WQ
Zeng
Wei
W
Zhang
Zai-rong
ZR
Li
Hui
H
Jiang
Si-ping
SP
Yang
Zhen
Z
Chen
Jin-rui
JR
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002448:Cell Adhesion; D002522:Chlorocebus aethiops; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology; D010946:Plants, Medicinal / Q000737:chemistry; D029623:Primulaceae / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014709:Vero Cells / Q000187:drug effects / Q000821:virology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D006023:Glycoproteins; D012333:RNA, Messenger