[Genetic analysis of a heading-delayed mutant by T-DNA insertion in rice (Oryza sativa L.)].
Transposon tagging was used to isolate genes in higher plant. In this study, a delayed heading mutant caused by T-DNA insertion in rice was identified. Genetic analysis of the mutant showed that the three types of phenotype, normal heading, delayed heading and overly delayed heading in the segregating populations derived from the T-DNA heterozygotes fit the ratio of 1:2:1. Test for Basta resistance showed the delayed heading plants were all resistant while the normal heading plants were susceptible, and the ratio of resistant and susceptible plants was 3:1, which indicated that the delayed heading mutant was co-segregated with Basta resistance. The delayed heading mutant caused by T-DNA insertion was confirmed by T-DNA detection using PCR method. This delayed heading mutant will be used for isolation of the tagged gene in rice.
Chen
Zhao-Gui
ZG
Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
Wang
Jiang
J
Zhang
Ze-Min
ZM
Liu
Fang
F
Zhu
Hai-Tao
HT
Wan
Xin-Shan
XS
Zhang
Jing-Liu
JL
Zhang
Gui-Quan
GQ
D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA