Mitochondrial DNA mutators.
In this article we review our current knowledge of the mechanisms by which point mutations arise in the mitochondrial DNA (mtDNA) of Saccharomyces cerevisiae and discuss to what extent these mechanisms operate in human mtDNA mutagenesis. The 3'-5' exonuclease proofreading activity of Pol gamma ensures accuracy of mtDNA replication in both yeast and humans, while the role of base excision repair in mtDNA error avoidance remains debated. The mitochondrial mismatch repair Msh1 protein, which removes transitions in yeast, is absent in humans, a particularity that might cause accumulation of transitions, while the most frequent substitution in yeast mtDNA is A:T to T:A transversion. Proofreading-deficient mutator human cell lines and knockin mice have been created. They will be useful for studying the mechanisms by which mtDNA mutations accumulate in diseases, ageing, malignancy and drug therapy.
Foury
F
F
Unité de Biochimie physiologique, Croix du Sud 2-20, 1348, Louvain-la-Neuve, Belgium. foury@fysa.ucl.ac.be
Hu
J
J
Vanderstraeten
S
S
D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000235:genetics; D016681:Genome, Fungal; D015894:Genome, Human; D006801:Humans; D016296:Mutagenesis; D017354:Point Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D004272:DNA, Mitochondrial; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases