Optimization of a multi-channel puffer system for rapid delivery of solutions during patch-clamp experiments.
In biological experiments, especially in neuroscience research, it is important to manipulate the extracellular environment efficiently. We have developed a micro-puffing system for local drug delivery to single cells in electrophysiological experiments, and validated the kinetic properties of this instrument. Based on our results, the kinetics of the delivery of solutions and the territory controlled by this system are influenced by several factors: (1) inner diameter (I.D.) of the guide tubing; (2) I.D. of the puffing tip; (3) angle of the puffing tip; and (4) gravity or external pressure applied to the solution. The system can fully control a territory of 200 x 600 micrometer2. The minimum delay in response to drug delivery is 10-20 ms. Switching between different solutions takes less than 100 ms. The minimum volume of solution required by the system is 0.2 ml. Taken together, our results provide useful data for designing and using an efficient drug/solution delivery system in electrophysiological experiments.
Wu
Bei
B
Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Wang
Ye-Ming
YM
Xiong
Wei
W
Zheng
Liang-Hong
LH
Fu
Chong-Luo
CL
Bruce
Iain C
IC
Zhang
Chen
C
Zhou
Zhuan
Z
D000818:Animals; D016503:Drug Delivery Systems; D004594:Electrophysiology; D007700:Kinetics; D018408:Patch-Clamp Techniques; D010477:Perfusion; D013997:Time Factors