title: Hypotensive and toxicological study of citric acid and other constituents from Tagetes patula roots.
abstract: Study of the effects of the methanolic extract of Tagetes patula roots on blood pressure led to the isolation of well known citric (1) and malic acid (7) as hypotensive, and pyridine hydrochloride (4) as hypertensive constituents of the plant along with a new constituent, 2-hydroxy, 5-hydroxymethyl furan (9). Citric acid and malic acid caused 71% and 43% fall in Mean Arterial Blood Pressure (MABP) of rats at the doses of 15 mg/kg and 30 mg/kg respectively while pyridine hydrochloride produced 34% rise in the MABP of rats at the dose of 30 mg/kg. LD50 and LD100 of citric acid in mice have been determined as 545 mg/kg and 1000 mg/kg, respectively.
authors.affiliation: Dr. HMI Institute of Pharmacology and Herbal Sciences, Hamdard University, Karachi-74600, Pakistan. rs127pk@yahoo.com
authors.forename: Rubeena
authors.initials: R
authors.lastname: Saleem
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ahmad
authors.forename: Aneela
authors.initials: A
authors.lastname: Naz
authors.forename: Humaira
authors.initials: H
authors.lastname: Siddiqui
authors.forename: Syed Iqbal
authors.initials: SI
authors.lastname: Ahmad
authors.forename: Shaheen
authors.initials: S
authors.lastname: Faizi
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D019343:Citric Acid / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008745:Methylation; D051379:Mice; D010936:Plant Extracts / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents; D031686:Tagetes / Q000737:chemistry* / Q000633:toxicity*
chemical_list: D000959:Antihypertensive Agents; D010936:Plant Extracts; D012997:Solvents; D019343:Citric Acid