Macrophage inflammatory protein-2, neutrophil recruitment and bacterial persistence in an experimental mouse model of urinary tract infection.
This study analyzed macrophage inflammatory protein-2 (MIP-2) production and neutrophil recruitment in urinary tract in response to Pseudomonas aeruginosa in an ascending model of urinary tract infection (UTI) in mice. Both planktonic and biofilm cells of P. aeruginosa were used for inducing UTI in mice. MIP-2 levels determined in urine, bladder and kidney showed maximum MIP-2 production 6 h postinfection, which correlated with neutrophil recruitment. Biofilm cells showed significantly more MIP-2 production and neutrophil recruitment. However, no correlation between bacterial numbers and neutrophil recruitment was observed in urine and kidney tissue. The role of MIP-2 and neutrophils in relation to the persistence of P. aeruginosa in the urinary tract of mice is discussed.
Mittal
Rahul
R
Department of Microbiology, Biomedical Sciences Block, Panjab University, Chandigarh 160014, India.
Chhibber
Sanjay
S
Sharma
Saroj
S
Harjai
Kusum
K
D000818:Animals; D054426:Chemokine CXCL2; D018925:Chemokines / Q000032:analysis / Q000096:biosynthesis* / Q000652:urine; D015169:Colony Count, Microbial; D005260:Female; D007668:Kidney / Q000276:immunology / Q000382:microbiology; D051379:Mice; D020556:Neutrophil Infiltration; D009195:Peroxidase / Q000378:metabolism; D011552:Pseudomonas Infections / Q000276:immunology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D001743:Urinary Bladder / Q000737:chemistry / Q000276:immunology / Q000382:microbiology; D014552:Urinary Tract Infections / Q000276:immunology / Q000382:microbiology*; D014556:Urine / Q000382:microbiology
D054426:Chemokine CXCL2; D018925:Chemokines; C404915:Cxcl2 protein, mouse; D009195:Peroxidase