Contribution of cholesteryl ester transfer protein and lecithin:cholesterol acyltransferase to HDL size distribution.
High-density lipoproteins (HDL) includes a heterogeneous class of lipoproteins grouped into various subclasses that seem to have different antiatherogenic function. Cholesteryl ester transfer protein (CETP) and lecithin cholesterol acyltransferase (LCAT) play an active role in HDL remodeling. This study was designed to define the role of CETP and LCAT activities on HDL-cholesterol (HDL-C) plasma levels and HDL size distribution, as determined by nondenaturating polyacrylamide gradient gel electrophoresis in 47 clinically healthy Mexican individuals without personal and family history of coronary heart disease. Surprisingly, plasma activities of CETP (29+/-4.1% of transfer) and LCAT (4.8+/-2.2% of esterification) did not correlate either with HDL-C plasma levels or with any other lipid parameter, indicating the poor contribution of these proteins to the lipid profile. The CETP activity showed a negative correlation with small HDL3b (r = -0476, P < 0.05), whereas LCAT was positively associated with this HDL subclass (r = 0.466, P < 0.05). The LCAT showed a negative correlation with large HDL2a (r = - 0.674, P < 0.005). Nevertheless, when the LCAT/CETP ratio was calculated, we observed that the higher the ratio, the greater the relative proportion of small HDL3b (r = 0.551, P < 0.05) and HDL3c (r = 0.477, P < 0.05). These results suggest that the balance of LCAT and CETP activities have a great impact in the plasma HDL size distribution.
Huesca-Gómez
Claudia
C
Physiology Department, Instituto Nacional de Cardiología Ignacio Chávez, México DF, Mexico.
Carreón-Torres
Elizabeth
E
Nepomuceno-Mejía
Tomás
T
Sánchez-Solorio
Marisela
M
Galicia-Hidalgo
Miguel
M
Mejía
Ana María
AM
Montaño
Luis-Felipe
LF
Franco
Martha
M
Posadas-Romero
Carlos
C
Pérez-Méndez
Oscar
O
D000293:Adolescent; D000328:Adult; D002352:Carrier Proteins / Q000097:blood* / Q000737:chemistry; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters / Q000097:blood* / Q000737:chemistry; D008076:Cholesterol, HDL / Q000032:analysis / Q000097:blood*; D002850:Chromatography, Gel; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood* / Q000737:chemistry; D012016:Reference Values
C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters; D008076:Cholesterol, HDL; D006023:Glycoproteins; D007862:Phosphatidylcholine-Sterol O-Acyltransferase