title: Reduction in adhesiveness to extracellular matrix components, modulation of adhesion molecules and in vivo migration of murine macrophages infected with Toxoplasma gondii.
abstract: Toxoplasma gondii is an obligate intracellular parasite, able to disseminate into deep tissues and cross biological barriers, reaching immunoprivileged sites such as the brain and retina. In order to investigate whether the parasite uses leukocyte trafficking to disseminate throughout the host, the adhesive potential to extracellular matrix components, the expression of adhesion molecules and the in vivo migration of murine macrophages infected with RH strain of T. gondii were investigated. Cellular adhesion to fibronectin, laminin and collagen IV decreased after 24 h of T. gondii infection. However, the decrease in adhesion of infected macrophages observed at early infection was reversed after 48 h. Moreover, decreased adhesion was dependent on active penetration, since heat-killed parasites were unable to reproduce it. Expression of integrins alphaL, alpha4 and alpha5 chains was downmodulated early postinfection, but a progressive regain of expression was observed after 12 h of infection. Expression of beta2, alphav and alpha4 integrins by peritoneal macrophages at late infection was also gradually reestablished. The assessment of in vivo migration of infected macrophages labeled with the fluorescent dye 5-chloromethylfluorescein diacetate showed a 48-h delay in migration to cervical lymph nodes when compared to LPS pre-stimulated macrophages. Furthermore, cells that migrate to distal lymph nodes were loaded with live parasites. Taken together, these results provide insights about T. gondii escape from the host immune response, placing the macrophage as a "Trojan horse", contributing to parasite dissemination and access to immunoprivileged sites.
authors.affiliation: Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Avenue Alberto Lamego, 2000, Rio de Janeiro CEP 28015-620, Brazil.
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Da Gama
authors.forename: Flávia Lima
authors.initials: FL
authors.lastname: Ribeiro-Gomes
authors.forename: Ubirajara
authors.initials: U
authors.lastname: Guimarães
authors.forename: Andrea C Vetö
authors.initials: AC
authors.lastname: Arnholdt
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000096:biosynthesis / Q000235:genetics; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology*; D024141:Collagen Type IV / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D039001:Integrin alpha Chains / Q000096:biosynthesis / Q000235:genetics; D007797:Laminin / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology; D008264:Macrophages / Q000469:parasitology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D014122:Toxoplasma / Q000472:pathogenicity*
chemical_list: D018821:CD18 Antigens; D024141:Collagen Type IV; D005353:Fibronectins; D039001:Integrin alpha Chains; D007797:Laminin