title: Neutrophils secrete MIP-1 beta after adhesion to laminin contained in basement membrane of blood vessels.
abstract: We have recently demonstrated that granulocyte colony-stimulating factor (G-CSF) stimulated the production of epithelial-cell-derived-neutrophil attractant-78 (ENA-78) by neutrophils and that ENA-78 might promote the accumulation of neutrophils that had migrated from the intravascular space into inflammatory tissues. In this study, we examined whether other chemokines could be secreted by neutrophils that had accumulated after migrating from the intravascular space into the inflammatory tissues. We demonstrated that adhesion to laminin contained in the basement membrane and Matrigel, which is an artificial basement membrane model, induced macrophage inflammatory protein-1 beta (MIP-1 beta) in neutrophils and that MIP-1 beta secreted by neutrophils induced the chemotaxis of dendritic cells. These findings suggest that neutrophils transmigrating through the basement membrane are stimulated to secrete MIP-1 beta by the basement membrane, inducing the transmigration of dendritic cells from the intravascular space into inflammatory tissues. We propose that neutrophils intervene between innate immune response and specific immune response by secreting MIP-1 beta during the transmigration through the basement membrane.
authors.affiliation: Department of Gastroenterology and Hematology, Hokkaido University Graduate School of Medicine, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Chiba
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhao
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.forename: Jingxin
authors.initials: J
authors.lastname: Wang
authors.forename: Hong Yan
authors.initials: HY
authors.lastname: Cui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawakami
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Miseki
authors.forename: Hashino
authors.initials: H
authors.lastname: Satoshi
authors.forename: Junji
authors.initials: J
authors.lastname: Tanaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asaka
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D001485:Basement Membrane / Q000378:metabolism*; D001808:Blood Vessels / Q000378:metabolism / Q000648:ultrastructure*; D002448:Cell Adhesion; D002478:Cells, Cultured; D054407:Chemokine CCL4; D002634:Chemotaxis, Leukocyte; D003094:Collagen; D003713:Dendritic Cells / Q000276:immunology; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D019402:Macrophage Inflammatory Proteins / Q000235:genetics / Q000378:metabolism*; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism*; D011509:Proteoglycans; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D054407:Chemokine CCL4; D004338:Drug Combinations; D007797:Laminin; D019402:Macrophage Inflammatory Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; C059485:matrigel; D003094:Collagen