Silencing of c-myc expression in tumor cells by siRNA.
Suppression of c-myc protooncogene expression in KB-3-1 cells by siRNA was investigated. The siRNA duplex targeted to the exon 3 of c-myc mRNA was prepared by in vitro transcription with T7 RNA polymerase on short dsDNA-templates. It was found that incubation of KB-3-1 cells in the presence of 75 nM siRNA results in decrease of the c-myc mRNA level down to 5% of the level in the control cells and significant decline of KB-3-1 cell proliferation rate. Using 200 nM siRNA four-fold decrease of KB-3-1 cells proliferation rate was observed and this effect was stable at least 96 h after transfection.
Kabilova
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TO
Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. pimanova@mail.ru
Chernolovskaya
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Vladimirova
A V
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Vlassov
V V
VV
D001483:Base Sequence; D002455:Cell Division; D045744:Cell Line, Tumor; D017931:DNA Primers; D020868:Gene Silencing / Q000502:physiology*; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D034741:RNA, Small Interfering / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering