Uncovering novel cell cycle players through the inactivation of securin in budding yeast.
Budding yeast securin/Pds1p, an inhibitor of the anaphase activator separase/Esp1p, is involved in several checkpoint pathways and in promoting Esp1p's nuclear localization. Using a modified synthetic genetic array (SGA) screen for genes that become essential in the absence of Pds1p, we uncovered roles for uncharacterized genes in cell cycle processes, including Esp1p activation.
Sarin
Sumeet
S
The Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0840, USA.
Ross
Karen E
KE
Boucher
Lorrie
L
Green
Yvette
Y
Tyers
Mike
M
Cohen-Fix
Orna
O
D002453:Cell Cycle / Q000235:genetics* / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D005804:Genes, Lethal; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D004718:Saccharomycetales / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D064249:Securin
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C578004:PDS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D064249:Securin