title: Efficient cleavage of RNA, enhanced cellular uptake, and controlled intracellular localization of conjugate DNAzymes.
abstract: Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency (k(cat)/K(m)) and enhanced tolerance against DNase 1digestion. To be pointed out, intracellular localization of DNAzymes could be controlled by conjugated with naturally occurring signal peptides responsible for nuclear cytoplasmic transport of proteins.
authors.affiliation: Department of Biological and Environmental Chemistry, School of Science and Technology, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Kubo
authors.forename: Kengo
authors.initials: K
authors.lastname: Takamori
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kanno
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
authors.forename: Bakalova
authors.initials: B
authors.lastname: Rumiana
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohba
authors.forename: Miho
authors.initials: M
authors.lastname: Matsukisono
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Akebiyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
mesh_terms: D001483:Base Sequence; D021881:DNA, Catalytic / Q000378:metabolism*; D015683:Gene Products, gag / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D006868:Hydrolysis; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*
chemical_list: D021881:DNA, Catalytic; D015683:Gene Products, gag; D012367:RNA, Viral