title: Bioeffect of ultrasound on endothelial cells in vitro.
abstract: The effects of low-intensity ultrasound (US) on biological systems have been investigated extensively; however, the effects of ultrasound stimulation on endothelial cells were rarely studied. In this study, 1 MHz, pulsed 1:4, and four different spatial-average temporal-peak intensities (0.5, 1.0, 1.6, and 2W/cm2) of ultrasound were used to stimulate endothelial cells for 10 min per day. The results showed that ultrasound (intensity 1.6-2.0W/cm2) treatment after 6 days enhanced the nitric oxide (NO) and Ca2+ release from the endothelial cells but did not promote cell growth. In addition, ultrasound stimulation changed the cellular morphology and orientation, and increased extracellular matrix secretion from endothelial cells.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 40227, Taiwan ROC. shhsu@nchu.edu.tw
authors.forename: Shan-hui
authors.initials: SH
authors.lastname: Hsu
authors.forename: Tsung-bin
authors.initials: TB
authors.lastname: Huang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000502:physiology / Q000528:radiation effects* / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D042783:Endothelial Cells / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology* / Q000528:radiation effects*; D005109:Extracellular Matrix / Q000502:physiology / Q000528:radiation effects* / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism*; D011829:Radiation Dosage; D014465:Ultrasonics; D014463:Ultrasonography
chemical_list: D009569:Nitric Oxide; D002118:Calcium