title: Regulation of RELM/FIZZ isoform expression by Cdx2 in response to innate and adaptive immune stimulation in the intestine.
abstract: Host immune responses to commensal flora and enteric pathogens are known to influence gene expression in the intestinal epithelium. Although the Cdx family of caudal-related transcription factors represents critical regulators of gene expression in the intestinal epithelium, the effect of intestinal immune responses on Cdx expression and function has not been determined. We have shown that bacterial colonization and Th2 immune stimulation by intestinal nematode infection induce expression of the intestinal goblet cell-specific gene RELM beta. In this study, we investigated the transcriptional regulation of resistin-like molecule/found in inflammatory zone (RELM/FIZZ, RELM beta) and its isoforms RELM alpha and RELM gamma to ascertain the role of Cdx in modifying intestinal gene expression associated with innate and adaptive immune responses. Analysis of the RELM beta promoter showed that Cdx2 plays a critical role in basal gene activation in vitro. This was confirmed in vivo using transgenic mice, where ectopic gastric and hepatic expression of Cdx2 induces expression of RELM beta, but not RELM alpha or RELM gamma, exclusively in the stomach. Although there was no quantitative change in colonic Cdx2 mRNA expression, protein distribution, or phosphorylation of Cdx2, bacterial colonization induced expression of RELM beta, but not RELM alpha or RELM gamma. In contrast, parasitic nematode infections activated colonic expression of all three RELM isoforms without alteration in Cdx2 expression. These results demonstrated that Cdx2 participates in directing intestine-specific expression of RELM beta in the presence of commensal bacteria and that adaptive Th2 immune responses to intestinal nematode infections can activate intestinal goblet cell-specific gene expression independent of Cdx2.
authors.affiliation: Division of Gastroenterology and Nutrition, The Children's Hospital of Philadelphia, Pennsylvania 19104-6144, USA.
authors.forename: Mei-Lun
authors.initials: ML
authors.lastname: Wang
authors.forename: Marcus E
authors.initials: ME
authors.lastname: Shin
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Knight
authors.forename: David
authors.initials: D
authors.lastname: Artis
authors.forename: Debra G
authors.initials: DG
authors.lastname: Silberg
authors.forename: Eunran
authors.initials: E
authors.lastname: Suh
authors.forename: Gary D
authors.initials: GD
authors.lastname: Wu
mesh_terms: D000818:Animals; D000071616:CDX2 Transcription Factor; D045744:Cell Line, Tumor; D005753:Gastric Mucosa / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D018398:Homeodomain Proteins / Q000502:physiology*; D006729:Hormones, Ectopic / Q000096:biosynthesis*; D006801:Humans; D018928:Immunity, Mucosal / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009349:Nematode Infections; D020932:Nerve Growth Factor / Q000096:biosynthesis; D020033:Protein Isoforms; D011506:Proteins; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: C491273:CDX1 protein, human; D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; C491272:Cdx1 protein, mouse; C487514:Cdx2 protein, mouse; D018398:Homeodomain Proteins; D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; D020033:Protein Isoforms; D011506:Proteins; C486574:RETNLB protein, human; C413576:Retnla protein, mouse; C486522:Retnlb protein, mouse; D014157:Transcription Factors; C482058:resistin-like gamma, mouse; D020932:Nerve Growth Factor