title: The stability of eukaryotic initiation factor 2-associated glycoprotein, p67, increases during skeletal muscle differentiation and that inhibits the phosphorylation of extracellular signal-regulated kinases 1 and 2.
abstract: Eukaryotic initiation factor 2-associated glycoprotein, p67, protects eIF2 from phosphorylation by its kinases. To understand the roles of p67 during skeletal muscle differentiation of mouse C2C12 myoblasts, we measured the level of p67 during myotube formation. We noticed that the level of p67 increases during myoblast differentiation and this increased level is controlled at the translational stage. The stability of p67 in the myotubes is due to its low turnover rate. The phosphorylation of the extracellular signal-regulated kinases (ERKs 1 and 2) is high in growth-factor-mediated cycling of C2C12 myoblasts and this phosphorylation decreases at 96 h when these myoblasts are grown in differentiation medium. At this time of differentiation, the level of p67 is higher compared to 0 h of differentiation. p67 binds to ERK2 and inhibits its activity in vitro. Taken together, these results suggest that the stability of p67 increases during myotube formation while inhibiting the phosphorylation of ERKs 1 and 2.
authors.affiliation: Department of Chemistry, Kent State University, Kent, OH 44242, United States. bdatta@kent.edu
authors.forename: Bansidhar
authors.initials: B
authors.lastname: Datta
authors.forename: Rekha
authors.initials: R
authors.lastname: Datta
authors.forename: Avijit
authors.initials: A
authors.lastname: Majumdar
authors.forename: Arnab
authors.initials: A
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D015852:Eukaryotic Initiation Factor-2; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D048049:Extracellular Signal-Regulated MAP Kinases