title: Proteomic analysis of in vivo phosphorylated synaptic proteins.
abstract: In the nervous system, protein phosphorylation is an essential feature of synaptic function. Although protein phosphorylation is known to be important for many synaptic processes and in disease, little is known about global phosphorylation of synaptic proteins. Heterogeneity and low abundance make protein phosphorylation analysis difficult, particularly for mammalian tissue samples. Using a new approach, combining both protein and peptide immobilized metal affinity chromatography and mass spectrometry data acquisition strategies, we have produced the first large scale map of the mouse synapse phosphoproteome. We report over 650 phosphorylation events corresponding to 331 sites (289 have been unambiguously assigned), 92% of which are novel. These represent 79 proteins, half of which are novel phosphoproteins, and include several highly phosphorylated proteins such as MAP1B (33 sites) and Bassoon (30 sites). An additional 149 candidate phosphoproteins were identified by profiling the composition of the protein immobilized metal affinity chromatography enrichment. All major synaptic protein classes were observed, including components of important pre- and postsynaptic complexes as well as low abundance signaling proteins. Bioinformatic and in vitro phosphorylation assays of peptide arrays suggest that a small number of kinases phosphorylate many proteins and that each substrate is phosphorylated by many kinases. These data substantially increase existing knowledge of synapse protein phosphorylation and support a model where the synapse phosphoproteome is functionally organized into a highly interconnected signaling network.
authors.affiliation: Division of Neuroscience, University of Edinburgh, Edinburgh EH8 9JZ, UK.
authors.forename: Mark O
authors.initials: MO
authors.lastname: Collins
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.forename: Marcelo P
authors.initials: MP
authors.lastname: Coba
authors.forename: Holger
authors.initials: H
authors.lastname: Husi
authors.forename: Iain
authors.initials: I
authors.lastname: Campuzano
authors.forename: Walter P
authors.initials: WP
authors.lastname: Blackstock
authors.forename: Jyoti S
authors.initials: JS
authors.lastname: Choudhary
authors.forename: Seth G N
authors.initials: SG
authors.lastname: Grant
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D019295:Computational Biology; D051379:Mice; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D040081:Protein Array Analysis; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D040901:Proteomics; D012995:Solubility / Q000187:drug effects; D013569:Synapses / Q000737:chemistry* / Q000378:metabolism; D013574:Synaptosomes / Q000737:chemistry; D014508:Urea / Q000494:pharmacology
chemical_list: D010750:Phosphoproteins; D014508:Urea; D011494:Protein Kinases