Estrogen attenuates P2X7-R-mediated apoptosis of uterine cervical cells by blocking calcium influx.
Estrogen blocks apoptosis of human ectocervical epithelial cells by modulating P2X7/Ca2+ influx. The effect involves decreased Ca2+-influx and cytosolic-calcium-increase via ATP-activated P2X7 pores. This mechanism may have physiological significance in the human cervix, in-vivo, and the results suggest a physiological role for estrogen in the cervix as an anti-apoptotic factor.
Gorodeski
George I
GI
Department of Obstetrics and Gynecology, University Hospitals of Cleveland, Cleveland, Ohio 44106, USA.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D003470:Culture Media / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D053938:DNA Fragmentation; D004967:Estrogens / Q000378:metabolism*; D004996:Ethidium / Q000494:pharmacology; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058486:Receptors, Purinergic P2X7; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology*
D003470:Culture Media; D004967:Estrogens; C546468:P2RX7 protein, human; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; C033901:3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate; D004996:Ethidium; D002118:Calcium