Subnucleolar distribution and organization of Vicia faba L. rDNA in situ.
The distribution and organization of nucleolar DNA in Vicia faba L. was analyzed by specific cytochemical staining using NAMA-Ur. The results showed that nucleolar DNA was distributed in the FCs and at the FC/DFC junctions. Statistical analysis showed that the rRNA genes occupied about one-third of the total dense fibrillar component region. The rDNA was condensed in some regions and uncondensed in others. Nucleolus-associated chromatin extended from outside the nucleolus to the periphery of the FCs via nucleolar channels, suggesting a possible origin for nucleolar DNA.
Long
Hong
H
Institute of Genetics and Cell Biology, Northeast Normal University, Changchun 130024, China.
Sun
Haijing
H
Zeng
Xianlu
X
Hao
Shui
S
Jiao
Mingda
M
D002466:Cell Nucleolus / Q000235:genetics* / Q000648:ultrastructure*; D002843:Chromatin / Q000235:genetics / Q000648:ultrastructure; D004396:Coloring Agents; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D006651:Histocytochemistry / Q000379:methods; D046529:Microscopy, Electron, Transmission; D009942:Organometallic Compounds; D018517:Plant Roots / Q000235:genetics / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods*; D014158:Transcription, Genetic / Q000235:genetics; D031307:Vicia faba / Q000235:genetics* / Q000648:ultrastructure
D002843:Chromatin; D004396:Coloring Agents; D004275:DNA, Ribosomal; D009942:Organometallic Compounds; C005460:uranyl acetate