title: [Genetic regulation of pathogenicity and virulence factors in bacteria Erwinia carotovora subsp. atroseptica: phenotypic characteristic of bacteria with the mutant kduD gene].
abstract: In contrast to the closely related bacteria Erwinia chrysanthemi, bacteria Erwinia carotovora subsp. atroseptica produce lower levels of main pathogenicity and virulence factors (pectate lyases, cellulases, and proteases) in the presence of pectins. This effect was shown to be connected with the accumulation of the intermediate product of intracellular degradation of these substances, 2,5-diketo-3-deoxygluconate (DK2). The presence of DK2 in the culture broth of mutant bacteria, connected to its export in the environment, was established. The production of pectate lyases, cellulases, and proteases is repressed by DK2 only at its high concentrations in the cultivation medium, whereas low concentrations of DK2 induce the production of virulence factors. Genes involved in the intracellular catabolism of pectin substances and induced by both low and high DK2 concentrations in the cultivation medium are not repressed by this metabolite.
authors.forename: V E
authors.initials: VE
authors.lastname: Miamin
authors.forename: A G
authors.initials: AG
authors.lastname: Pesniakevich
authors.forename: V A
authors.initials: VA
authors.lastname: Prokulevich
mesh_terms: D002480:Cellulase / Q000096:biosynthesis / Q000172:deficiency; D003001:Cloning, Molecular; D016974:Dickeya chrysanthemi / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005942:Gluconates / Q000378:metabolism*; D009154:Mutation; D010368:Pectins / Q000378:metabolism; D016973:Pectobacterium carotovorum / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000172:deficiency; D010641:Phenotype; D011133:Polysaccharide-Lyases / Q000096:biosynthesis / Q000172:deficiency; D014774:Virulence
chemical_list: C499105:2,5-diketo-3-deoxygluconate; D005942:Gluconates; D010368:Pectins; D002480:Cellulase; D010447:Peptide Hydrolases; D011133:Polysaccharide-Lyases; C023911:pectate lyase