Effects of hypoxanthine on adenosine transport in human lymphocytes. Implications in the pathogenesis of Lesch-Nyhan syndrome.
We have examined the effect of hypoxanthine on adenosine transport and [3H] NBTI binding in peripheral blood lymphocytes (PBL) cultures. Pre-incubation with hypoxanthine originates a dose dependent decrease of adenosine transport and [3H] NBTI binding sites in PBL.
Torres
R J
RJ
Biochemistry Laboratory, La Paz University Hospital, Madrid, Spain.
DeAntonio
I
I
Prior
C
C
Puig
J G
JG
D000241:Adenosine / Q000097:blood* / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019271:Hypoxanthine / Q000494:pharmacology*; D007926:Lesch-Nyhan Syndrome / Q000097:blood* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D019271:Hypoxanthine; D000242:Cyclic AMP; D000241:Adenosine