title: Post-transcriptional effects of phorbol 12-myristate 13-acetate on transcriptome of U937 cells.
abstract: To identify post-transcriptionally modulated genes at the translational level by phorbol 12-myristate 13-acetate (PMA), we investigated mRNA profiles in the polysomal and the cytoplasmic fractions of U937 cells before and after PMA stimulation using microarrays with 15017 oligonucleotide probes. Global comparison of the profiles showed that the cytoplasmic distribution of mRNAs was considerably modulated upon PMA stimulation. The results also indicate that PMA post-transcriptionally regulated at least 0.7% of detectable genes in U937 cells. Thus, besides transcriptional modulation by PMA, changes in the translational state of transcripts seem to play a critical role in PMA-induced differentiation of U937 cells.
authors.affiliation: Laboratory for Immunogenomics, Research Center for Allergy and Immunology, The Institute of Physical and Chemical Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitamura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Michiyo
authors.initials: M
authors.lastname: Takayama
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Kimura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hijikata
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hijika
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohara
mesh_terms: D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011132:Polyribosomes / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D013347:Subcellular Fractions; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic; D020298:U937 Cells
chemical_list: D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate