title: Silencing of MDR 1 gene in cancer cells by siRNA.
abstract: Inhibition of p-glycoprotein (PGP) expression and reverse of multidrug resistance (MDR) phenotype in KB-8-5 cells by synthetic 21-bp double-stranded oligoribonucleotides were investigated. siRNA constructs for the efficient down regulation of MDR1 that are active in nanomolar concentrations and cause reversal of MDR phenotype in cells were developed.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. evg_log@niboch.nsc.ru
authors.forename: E B
authors.initials: EB
authors.lastname: Logashenko
authors.forename: A V
authors.initials: AV
authors.lastname: Vladimirova
authors.forename: M N
authors.initials: MN
authors.lastname: Repkova
authors.forename: A G
authors.initials: AG
authors.lastname: Venyaminova
authors.forename: E L
authors.initials: EL
authors.lastname: Chernolovskaya
authors.forename: V V
authors.initials: VV
authors.lastname: Vlassov
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D020868:Gene Silencing / Q000502:physiology*; D019450:Genes, MDR; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000502:physiology*; D020112:Rhodamine 123 / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D020112:Rhodamine 123