Identification and characterization of a Ca2+ -sensitive interaction of the vanilloid receptor TRPV1 with tubulin.
The vanilloid receptor TRPV1 plays a well-established functional role in the detection of a range of chemical and thermal noxious stimuli, such as those associated with tissue inflammation and the resulting pain. TRPV1 activation results in membrane depolarization, but may also trigger intracellular Ca2+ -signalling events. In a proteomic screen for proteins associated with the C-terminal sequence of TRPV1, we identified beta-tubulin as a specific TRPV1-interacting protein. We demonstrate that the TRPV1 C-terminal tail is capable of binding tubulin dimers, as well as of binding polymerized microtubules. The interaction is Ca2+ -sensitive, and affects microtubule properties, such as microtubule sensitivity towards low temperatures and nocodazole. Our data thus provide compelling evidence for the interaction of TRPV1 with the cytoskeleton. The Ca2+ -sensitivity of this interaction suggests that the microtubule cytoskeleton at the cell membrane may be a downstream effector of TRPV1 activation.
Goswami
C
C
Freie Universität Berlin, Institute für Chemie-Biochemie, Berlin, Germany.
Dreger
M
M
Jahnel
R
R
Bogen
O
O
Gillen
C
C
Hucho
F
F
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D058619:Maltose-Binding Proteins; D008954:Models, Biological; D010590:Phalloidine / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013116:Spinal Cord / Q000378:metabolism; D013552:Swine; D050916:TRPV Cation Channels; D013696:Temperature; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism*
D002352:Carrier Proteins; D007473:Ion Channels; D058619:Maltose-Binding Proteins; D011994:Recombinant Proteins; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D014404:Tubulin; D010590:Phalloidine; D002118:Calcium