title: [Relationship between acute dipterex poisoning and oxidative stress and free radical damage].
abstract: OBJECTIVE
  To study relationship between acute dipterex poisoning and oxidative stress and free radical damage.
  
  
  METHODS
  Eighty-two patients with acute dipterex poisoning (ADPP) and ninety-two healthy adult volunteers (HAV) were enrolled in the study with randomized controlled trial design. Plasma levels of vitamin C (VC) and vitamin E (VE), as well as level of lipoperoxide (LPO) and activities of superoxide dismutase (SOD) and acetylcholinesterase (AChE) in the red blood cells (RBC), were determined by spectrophotometry.
  
  
  RESULTS
  Levels of VC and VE, and activities of SOD and AChE were (37.35 +/- 9.98) micromol/L, (16.57 +/- 4.54) micromol/L, (1 785 +/- 154) U/g Hb and (213.1 +/- 57.6) U/g Hb, respectively, in the ADPP group, significantly lower than those in the HAV group, (55.34 +/- 15.98) micromol/L, (25.66 +/- 7.24) micromol/L, (2 124 +/- 185) U/g Hb and (305.3 +/- 83.6) U/g Hb, respectively. Plasma level of LPO was (35.20 +/- 5.29) nmol/g Hb in the ADPP group, significantly higher than that in the HAV group, (27.87 +/- 4.66) nmol/g Hb. Partial correlation analysis suggested that there existed negative correlation between activity of AChE in the RBC and plasma level of LPO (r = -0.274, P = 0.013) and positive correlation between activity of AChE in the RBC and plasma levels of VC and VE, and activity of SOD in the RBC (r = 0.333, P = 0.002, r = 0.269, P = 0.015 and r = 0.248, P = 0.026, respectively) in the ADPP, adjusted for age. Coefficient of reliability alpha was 0.682 (P < 0.001), with a standardized alpha of 0.868 (P < 0.001).
  
  
  CONCLUSIONS
  There exist severe oxidative stress and free radical damage in patients with acute dipterex poisoning.
authors.affiliation: Department of Internal Medicine, Pujiang County People's Hospital, Zhejiang 322200, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Rui
authors.forename: Jun-fu
authors.initials: JF
authors.lastname: Zhou
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Shao
authors.forename: Xue-ying
authors.initials: XY
authors.lastname: Zhang
authors.forename: Juan-fei
authors.initials: JF
authors.lastname: He
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000293:Adolescent; D000328:Adult; D001205:Ascorbic Acid / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005609:Free Radicals; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D011041:Poisoning / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood*; D014236:Trichlorfon / Q000506:poisoning*; D014810:Vitamin E / Q000097:blood
chemical_list: D005609:Free Radicals; D014810:Vitamin E; D014236:Trichlorfon; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D001205:Ascorbic Acid; D010100:Oxygen