Dystroglycan is involved in laminin-1-stimulated motility of Müller glial cells: combined velocity and directionality analysis.
We investigate the role of dystroglycan, a major laminin-1 receptor and central member of the dystrophin-glycoprotein complex, in the laminin-1 induced motility of cultured Muller glial cells. Binding of laminin-1 to dystroglycan was prevented by IIH6, a function-blocking monoclonal antibody against alpha-dystroglycan. As an alternative means of inhibition, we used heparin to mask the dystroglycan binding site of the laminin-1, known to overlap with heparin binding sites. Cell motility was characterized in a two-dimensional motility assay based on computer-controlled videomicroscopy and statistical analysis of cellular trajectories. We obtained data on both the cell velocity and the diffusion index, a measure of direction-changing frequency. Both means of inhibition of dystroglycan function led to a significant decrease in the ability of laminin-1 to stimulate cell migration. At the same time, dystroglycan function does not appear to be involved in laminin-1-dependent increase in process dynamism and direction-changing activity.
Méhes
Elöd
E
Department of Anatomy and Histology, Faculty of Veterinary Science, Szent István University, Budapest, Hungary.
Czirók
András
A
Hegedüs
Balázs
B
Szabó
Bálint
B
Vicsek
Tamás
T
Satz
Jakob
J
Campbell
Kevin
K
Jancsik
Veronika
V
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D049030:Dystroglycans / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D007797:Laminin / Q000378:metabolism* / Q000494:pharmacology; D018715:Microscopy, Video; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D000906:Antibodies; D007797:Laminin; C121346:laminin 1; D049030:Dystroglycans; D006493:Heparin