High expression during neurogenesis but not mammogenesis of a murine homologue of the Deleted in Breast Cancer2/Rhobtb2 tumor suppressor.
Deleted in Breast Cancer-2, Dbc2, identified as a candidate tumor suppressor gene for breast cancer and other human malignancies, is a member of a novel gene family encoding putative small GTPases designated as Rhobtb. In this report, we describe the spatio-temporal expression of mDbc2/Rhobtb2 mRNA during mammary gland development and embryogenesis in the mouse. We have first determined that mDbc2/Rhobtb2 transcripts are expressed at low, but seemingly constant levels during mammopoiesis. Secondly, we have found that mDbc2/Rhobtb2 is expressed highly and specifically in the central and peripheral nervous systems during mouse embryogenesis. While our results do not reveal any connection between elevated expression of mDbc2/Rhobtb2 and a specific stage of mammary gland development, they strongly support a role for this gene during development of the nervous system.
St-Pierre
Benoit
B
Division of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, Toronto, Ont., Canada M5G 2M1.
Jiang
Zhe
Z
Egan
Sean E
SE
Zacksenhaus
Eldad
E
D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D020558:GTP Phosphohydrolases / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008321:Mammary Glands, Animal / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D009024:Morphogenesis; D017933:Peripheral Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
D025521:Tumor Suppressor Proteins; D020558:GTP Phosphohydrolases