title: Development and evaluation of the essential oil from Magnolia fargesii for enhancing the transdermal absorption of theophylline and cianidanol.
abstract: To improve the skin permeation of theophylline and cianidanol ((+)-catechin), the essential oil of Magnolia fargesii was evaluated using in-vitro and in-vivo permeation techniques. Oxygenated monoterpenes and sesquiterpenes are the major components of M. fargesii essential oil. The in-vitro permeation of theophylline and cianidanol was significantly enhanced after treatment with M. fargesii essential oil. The essential oil increased the in-vivo skin deposition of cianidanol but not theophylline. On the other hand, in-vivo microdialysis showed a higher subcutaneous theophylline amount after essential oil treatment. In-vitro cell viability and prostaglandin E(2) release by skin keratinocytes indicated that there was low or negligible cytotoxicity by M. fargesii essential oil. The in-vivo skin tolerance study determined by transepidermal water loss and colorimetry confirmed that no irritation of the skin was detected when using M. fargesii essential oil.
authors.affiliation: Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 333, Taiwan. fajy@mail.cgu.edu.tw
authors.forename: Jia-You
authors.initials: JY
authors.lastname: Fang
authors.forename: Tung-Hu
authors.initials: TH
authors.lastname: Tsai
authors.forename: Chi-Feng
authors.initials: CF
authors.lastname: Hung
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Wong
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002392:Catechin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D018929:Cell Culture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004357:Drug Synergism; D005260:Female; D006801:Humans; D031566:Magnolia; D009822:Oils, Volatile / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology*; D013806:Theophylline / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D009822:Oils, Volatile; D002392:Catechin; D013806:Theophylline