title: Discrepancy between sperm acrosin activity and sperm morphology: significance for fertilization in vitro.
abstract: BACKGROUND
  In routine semen analysis, discrepancies may occur between sperm acrosin activity test results and sperm acrosomal morphology.
  
  
  METHODS
  Discrepant test results of sperm acrosin activity (spectrophotometric assay) vs. sperm morphology (strict criteria) in the initial diagnostic investigation of 107 infertile couples were evaluated with respect to fertilization rate (% oocytes with 2 pronuclei) further obtained in IVF treatment.
  
  
  RESULTS
  Acrosin activity positively correlated with sperm morphology (% normal forms) (r=0.537) and fertilization rate (r=0.526). ROC curves for the prediction of > or =50% fertilization rate were comparable for acrosin activity and sperm morphology, with optimal cutoff values at 25 microIU/10(6) sperm and 10%, respectively. In multiple regression analysis, sperm acrosin activity (P=0.002) predicted fertilization rate independently of sperm morphology (P<0.001) and sperm vitality (eosin-nigrosin stain) (P=0.03). Acrosin activities > or =25 microIU/10(6) sperm were observed in 36% of severe teratozoospermic samples (< or =4% normal spermatozoa) associated with low fertilization rate. Twenty percent of the morphologically normal ejaculates showed a low acrosin activity (<25 microIU/10(6) sperm) and low hypoosmotic swelling test (HOST) scores (31.4+/-7.6%) and were associated with low fertilization rate.
  
  
  CONCLUSIONS
  The sperm acrosin assay can help to predict sperm fertilizing capacity in IVF independently of sperm morphology.
authors.affiliation: Andrology Unit, Department of Clinical Chemistry, AZ St-Jan Hospital, Ruddershove 10, B-8000 Bruges, Belgium. michel.langlois@azbrugge.be
authors.forename: Michel R
authors.initials: MR
authors.lastname: Langlois
authors.forename: Luc
authors.initials: L
authors.lastname: Oorlynck
authors.forename: Frank
authors.initials: F
authors.lastname: Vandekerckhove
authors.forename: Arnold
authors.initials: A
authors.lastname: Criel
authors.forename: Dirk
authors.initials: D
authors.lastname: Bernard
authors.forename: Victor
authors.initials: V
authors.lastname: Blaton
mesh_terms: D000176:Acrosin / Q000378:metabolism*; D000328:Adult; D048429:Cell Size; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D009994:Osmolar Concentration; D012189:Retrospective Studies; D013094:Spermatozoa / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure*
chemical_list: D000176:Acrosin