title: [T-bet gene expression in acute graft versus host disease].
abstract: OBJECTIVE
  To study the role of T-bet [a T helper 1 (Th1) lymphocyte transcription factor] gene expression in predicting acute graft-versus-host disease (aGVHD) and evaluate the correlation between T-bet gene and aGVHD.
  
  
  METHODS
  Twenty patients who underwent allogeneic stem cell transplantation (allo. HSCT) entered this study. The expression of T-bet gene was examined with reverse transcription (RT)-PCR in bone marrow samples collected from patients on the day before conditioning, and day 0, day 14, day 28, and day 42 after HSCT.
  
  
  RESULTS
  The expression level of T-bet in patients developed aGVHD was increased compared with that before conditioning (P = 0.043). The incidence of aGVHD was 91.7% in patients whose T-bet expression level was increased on day 14 after transplant while was 12.5% in those whose T-bet gene expression level was not increased on that day (P < 0.001).
  
  
  CONCLUSIONS
  Patients with increased expression levels of T-bet after allo-HSCT may have an increased possibility to develop aGVHD. T-bet expression level may serve as an advisable guide to the clinician in predicting aGVHD and monitoring treatment.
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Zhang-song
authors.initials: ZS
authors.lastname: Yan
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Liu
authors.forename: Dong-lin
authors.initials: DL
authors.lastname: Yang
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Zhou
authors.forename: Er-lie
authors.initials: EL
authors.lastname: Jiang
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: He-hua
authors.initials: HH
authors.lastname: Wang
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Zhai
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Si-zhou
authors.initials: SZ
authors.lastname: Feng
authors.forename: Ming-zhe
authors.initials: MZ
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000378:metabolism*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020825:T-Box Domain Proteins; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D000098815:T-bet Transcription Factor
chemical_list: D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014157:Transcription Factors