Identification of enzymes acting on alpha-glycated amino acids in Bacillus subtilis.
We have characterized the Bacillus subtilis homologs of fructoselysine 6-kinase and fructoselysine-6-phosphate deglycase, two enzymes that specifically metabolize the Amadori compound fructose-epsilon-lysine in Escherichia coli. The B. subtilis enzymes also catalyzed the phosphorylation of fructosamines to fructosamine 6-phosphates (YurL) and the conversion of the latter to glucose 6-phosphate and a free amino acid (YurP). However, their specificity was totally different from that of the E. coli enzymes, since they acted on fructoseglycine, fructosevaline (YurL) or their 6-phosphoderivatives (YurP) with more than 30-fold higher catalytic efficiencies than on fructose-alpha-lysine (6-phosphate). These enzymes are therefore involved in the metabolism of alpha-glycated amino acids.
Wiame
Elsa
E
Laboratory of Physiological Chemistry, ICP and Université Catholique de Louvain, P.O. Box 7539, Avenue Hippocrate 75, B-1200 Brussels, Belgium.
Duquenne
Armelle
A
Delpierre
Ghislain
G
Van Schaftingen
Emile
E
D000595:Amino Acid Sequence / Q000235:genetics; D000596:Amino Acids / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002384:Catalysis; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019270:Fructosamine / Q000737:chemistry / Q000378:metabolism; D005632:Fructose / Q000031:analogs & derivatives* / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding
D000596:Amino Acids; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006023:Glycoproteins; C033186:fructosyl-lysine; D005632:Fructose; D019270:Fructosamine; C033188:fructosyl-glycine; D008239:Lysine; D005998:Glycine