title: p-Iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias.
abstract: BACKGROUND
  In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).
  
  
  METHODS
  The frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.
  
  
  RESULTS
  All cases of AML-M7 and AML-M I exhibited integrated light emission greater than 73(10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-MO showed results above the cutoff point.
  
  
  CONCLUSIONS
  This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-MI cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.
authors.affiliation: Department of Clinical Analysis (LAC), School of Pharmaceutical Sciences, State University of São Paulo (UNESP), Araraquara, SP, Brazil.
authors.forename: Miriane
authors.initials: M
authors.lastname: da Costa
authors.forename: Valdecir Farias
authors.initials: VF
authors.lastname: Ximenes
authors.forename: Iguatemy Lourenço
authors.initials: IL
authors.lastname: Brunetti
authors.forename: Roberto Passetto
authors.initials: RP
authors.lastname: Falcão
authors.forename: Luiz Marcos
authors.initials: LM
authors.lastname: da Fonseca
mesh_terms: D001854:Bone Marrow Cells / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007462:Iodobenzenes / Q000737:chemistry*; D007947:Leukemia, Megakaryoblastic, Acute / Q000175:diagnosis* / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000473:pathology; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry* / Q000378:metabolism; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000473:pathology
chemical_list: D007462:Iodobenzenes; C050181:4-iodophenol; D008165:Luminol; D009195:Peroxidase