Peripheral blood fibrocytes contribute to the formation of the avian spleen.
Chick-quail chimeric studies were made to determine the origin of the cells of splenic ellipsoid. The ellipsoid is formed by supporting and phagocytic cells, which are embedded in a well-organized extracellular matrix. Splenic and bursal anlage of 6- to 6.5-day-old quail embryos were transplanted into the coelomic cavity of 3-day-old chick embryos and further incubated for 17 days. CD45+ chicken hemopoietic cells colonized both organs. They formed the cells of the ellipsoid and the periellipsoidal white pulp of the transplanted quail spleen. Chicken-specific collagen III was produced only in the donor quail spleen, but not in the bursa of Fabricius. The CD45+/collagen I+/collagen III+ cells are probably identical with the mammalian peripheral blood fibrocytes and contribute to the formation of supporting cells, whereas the CD45+/74.2+ ellipsoid-associated macrophages are of monocytic origin. We provide, for the first time, experimental evidence that peripheral blood fibrocytes exist in the avian species; they are present in the circulation of the chicken embryo and contribute to the organogenesis of the spleen.
Nagy
Nándor
N
Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
Bíró
Eva
E
Takács
Agnes
A
Pólos
Miklós
M
Magyar
Attila
A
Oláh
Imre
I
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis; D001773:Blood Cells / Q000166:cytology*; D002060:Bursa of Fabricius / Q000166:cytology*; D002454:Cell Differentiation; D017690:Cell Transplantation; D002642:Chick Embryo / Q000166:cytology; D002678:Chimera; D003094:Collagen / Q000378:metabolism; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis / Q000378:metabolism; D003239:Connective Tissue Cells / Q000166:cytology; D003370:Coturnix; D047108:Embryonic Development; D005109:Extracellular Matrix / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008953:Models, Anatomic; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology; D010587:Phagocytosis; D011784:Quail; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000196:embryology*; D013997:Time Factors
D000944:Antigens, Differentiation, B-Lymphocyte; D024042:Collagen Type I; D024061:Collagen Type III; D000949:Histocompatibility Antigens Class II; D011993:Recombinant Fusion Proteins; C045084:invariant chain; D003094:Collagen; D017493:Leukocyte Common Antigens