Distribution and in situ proliferation patterns of intravenously injected immortalized human neural stem-like cells in rats with focal cerebral ischemia.
Neural stem cells are considered as a candidate for cell replacement therapy in various neurological diseases. To investigate whether human neural stem cells can migrate into the adult ischemic rat brain, we transplanted immortalized human neural 'tem-like' cells intravenously 24 h after focal cerebral ischemia. The intravenously injected human neural stem-like cells were found around the infarcted area, differentiated into neurons and astrocytes in the lesioned areas, and survive up to 56 days after transplantation. The number of the injected cells increased between 7 and 14 days after transplantation with incorporating BrdU. Our findings show that intravenously injected human neural stem-like cells may incorporate into the ischemic brain, and undergo proliferation responding to the endogenous mitotic signal during the acute period of focal ischemia.
Chu
Kon
K
Department of Neurology, Clinical Research Institute, Seoul National University Hospital, 28 Yongon-Dong, Chongro-Gu, Seoul 110-744, South Korea.
Kim
Manho
M
Chae
Seung-Hee
SH
Jeong
Sang-Wuk
SW
Kang
Kyu-Sik
KS
Jung
Keun-Hwa
KH
Kim
Juhyun
J
Kim
Young-Ju
YJ
Kang
Lamie
L
Kim
Seung U
SU
Yoon
Byung-Woo
BW
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D015415:Biomarkers; D001921:Brain / Q000166:cytology / Q000502:physiology / Q000601:surgery; D002545:Brain Ischemia / Q000628:therapy*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002544:Cerebral Infarction / Q000628:therapy; D004195:Disease Models, Animal; D006085:Graft Survival / Q000502:physiology*; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D015415:Biomarkers