title: Effect of mash maceration on the polyphenolic content and visual quality attributes of cloudy apple juice.
abstract: The effects of enzymatic mash treatments on yield, turbidity, color, and polyphenolic content of cloudy apple juice were studied. Using HPLC-ESI-MS, cryptochlorogenic acid was identified in cv. Brettacher cloudy apple juice for the first time. Commercial pectolytic enzyme preparations with different levels of secondary protease activity were tested under both oxidative and nonoxidative conditions. Without the addition of ascorbic acid, oxidation substantially decreased chlorogenic acid, epicatechin, and procyanidin B2 contents due to enzymatic browning. The content of chlorogenic acid as the major polyphenolic compound was also influenced by the composition of pectolytic enzyme preparations because the presence of secondary protease activity resulted in a rise of chlorogenic acid. The latter effect was probably due to the inhibited protein-polyphenol interactions, which prevented binding of polyphenolic compounds to the matrix, thus increasing their antioxidative potential. The results obtained clearly demonstrate the advantage of the nonoxidative mash maceration for the production of cloud-stable apple juice with a high polyphenolic content, particularly in a premature processing campaign.
authors.affiliation: Institute of Food Technology, Section Plant Foodstuff Technology, Hohenheim University, August-von-Hartmann-Strasse 3, D-70599 Stuttgart, Germany.
authors.forename: Kiril
authors.initials: K
authors.lastname: Mihalev
authors.forename: Andreas
authors.initials: A
authors.lastname: Schieber
authors.forename: Plamen
authors.initials: P
authors.lastname: Mollov
authors.forename: Reinhold
authors.initials: R
authors.lastname: Carle
mesh_terms: D001628:Beverages; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003116:Color; D005419:Flavonoids / Q000032:analysis*; D005511:Food Handling / Q000379:methods*; D027845:Malus; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D002726:Chlorogenic Acid