A rapid microplate method for quantifying inhibition of bacterial adhesion to eukaryotic cells.
Adhesion of bacteria to the mucosal epithelial cell surface is the first step in infection, and studies have shown that inhibition of this step may be useful therapeutically. To test compounds that may prevent bacterial binding to a number of epithelial cell lines, we have developed a high-throughput adhesion assay using a microtitre plate system and bacteria that have been modified to express firefly luciferase. This method has proved to be a sensitive, rapid, and reproducible system for screening antiadhesive agents for their effects on bacterial adhesion.
Acord
John
J
Institute of Cell and Molecular Science, Centre for Infectious Disease, Barts and the London, Queen Mary's School of Medicine and Dentistry, London E1 2AD, UK. j.acord@qmul.ac.uk
Maskell
Jeff
J
Sefton
Armine
A
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D016956:Burkholderia cepacia / Q000235:genetics / Q000502:physiology*; D002241:Carbohydrates / Q000502:physiology; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic
D002241:Carbohydrates; D049409:Luciferases, Firefly