High-resolution NMR spectroscopy of membrane proteins in aligned bicelles.
High-resolution solid-state NMR spectra can be obtained from uniformly (15)N-labeled membrane proteins in magnetically aligned bicelles. Fast uniaxial diffusion about the axis of the bilayer normal results in single-line spectra that contain the orientational information necessary for protein structure determination.
De Angelis
Anna A
AA
Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307, USA.
Nevzorov
Alexander A
AA
Park
Sang Ho
SH
Howell
Stanley C
SC
Mrse
Anthony A
AA
Opella
Stanley J
SJ
D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry
D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D010767:Phosphorylcholine