Chiral separation of natural and unnatural amino acid derivatives by micro-HPLC on a Ristocetin A stationary phase.
This paper deals with the chiral separation of Fmoc- and Z-derivatives of natural and unnatural sulfur containing amino acids by micro-HPLC. The separations were carried out in microbore columns packed with a new material based on Ristocetin A bonded to 3.5 microm silica gel. The columns were run in the normal phase, polar organic mode as well as in the reversed phase mode, whereby best results were obtained with the reversed-phase mode using mixtures of triethylamine acetate (TEAA) buffer and methanol as mobile phases.
Piccinini
Anna-Maria
AM
Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Universitätsplatz 1, A-8010 Graz, Austria.
Schmid
Martin G
MG
Pajpanova
Tamara
T
Pancheva
Svetlana
S
Grueva
Ekatarina
E
Gübitz
Gerald
G
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification; D000603:Amino Acids, Sulfur / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry / Q000302:isolation & purification; D007536:Isomerism; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D012310:Ristocetin / Q000737:chemistry*
D000596:Amino Acids; D000603:Amino Acids, Sulfur; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D012310:Ristocetin; C016719:ristocetin A