Cross-catalytic replication of an RNA ligase ribozyme.
A self-replicating RNA ligase ribozyme was converted to a cross-catalytic format whereby two ribozymes direct each other's synthesis from a total of four component substrates. Each ribozyme binds two RNA substrates and catalyzes their ligation to form the opposing ribozyme. The two ribozymes are not perfectly complementary, as is the case for replicating nucleic acid genomes in biology. Rather, the ribozymes contain both template elements, which are complementary, and catalytic elements, which are identical. The specificity of the template interactions allows the cross-catalytic pathway to dominate over all other reaction pathways. As the concentration of the two ribozymes increases, the rate of formation of additional ribozyme molecules increases, consistent with the overall autocatalytic behavior of the reaction system.
Kim
Dong-Eun
DE
Department of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Joyce
Gerald F
GF
D001483:Base Sequence; D015195:Drug Design; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011130:RNA Ligase (ATP) / Q000096:biosynthesis*; D016337:RNA, Catalytic / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics
D016337:RNA, Catalytic; D011130:RNA Ligase (ATP)