Comparison of QTL controlling seedling vigour under different temperature conditions using recombinant inbred lines in rice (Oryza sativa).
OBJECTIVE
Seedling vigour is one of the major determinants for stable stand establishment in rice (Oryza sativa), especially in a direct seeding cropping system. The objectives of this study were to identify superior alleles with consistent effects on seedling vigour across different temperature conditions and to investigate genotype x environmental temperature interactions for seedling vigour QTL.


METHODS
A set of 282 F13 recombinant inbred lines (RILs) derived from a rice cross were assessed for four seedling vigour traits at three temperatures (25 degrees C, 20 degrees C and 15 degrees C). Using a linkage map with 198 marker loci, the main-effect QTL for the traits were mapped by composite interval mapping.


RESULTS
A total of 34 QTL for the four seedling vigour traits were identified. Of these QTL, the majority (82%) were clustered within five genomic regions, designated as QTL qSV-3-1, qSV-3-2, qSV-5, qSV-8-1 and qSV-8-2. All of these five QTL had small individual effects on the traits, explaining 3.1-15.8 % of the phenotypic variation with a mean of 7.3 %. QTL qSV-3-1, qSV-3-2 and qSV-8-1 showed almost consistent effects on the traits across all three temperatures while qSV-5 and qSV-8-2 had effects mainly at the 'normal' temperatures of 20 degrees C and 25 degrees C. Among the five QTL identified, all and four showed additive effects on shoot length and germination rate, respectively. The contributions of these five QTL to shoot length and germination rate were also much larger than those to the other two traits.


CONCLUSIONS
A few of genomic regions (or QTL) were identified as showing effects on seedling vigour. For these QTL, significant genotype x environmental temperature interactions were found and these interactions appeared to be QTL-specific. Among the four seedling vigour traits measured, shoot length and germination rate could be used as relatively good indicators to evaluate the level of seedling vigour in rice.
Zhang
Zhi-Hong
ZH
Key Laboratory of the Education Ministry of China for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Qu
Xin-Shun
XS
Wan
Shan
S
Chen
Li-Hua
LH
Zhu
Ying-Guo
YG
D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018525:Germination; D012275:Oryza / Q000235:genetics* / Q000502:physiology*; D010641:Phenotype; D030821:Plants, Genetically Modified; D040641:Quantitative Trait Loci / Q000502:physiology*; D036226:Seedlings / Q000502:physiology*; D012639:Seeds / Q000502:physiology; D013696:Temperature