[Study of HLA polymorphism in the 6965 Han bone marrow registry donors].
OBJECTIVE
To analyze human leukocyte antigen (HLA) polymorphism and search for new alleles in Chinese Han population bone marrow registry donors.


METHODS
DNA-based HLA genotyping methods were used including PCR-SSP, BST and molecular cloning.


RESULTS
A total of 6965 unrelated donors, 4707 from South China origin and 2258 from north, were typed for HLA-A, B, and DRB1 loci. Seventy-two specificities of HLA alleles were identified. The HLA-A25, A34, A74, B41, B42, B53, B73 and B81 that were rarely reported in previously Chinese population studies were identified in this study. Estimation of gene frequency indicated that the blank gene frequency was less than 0.2% for HLA-A, 0.25% for HLA-B and 0.70% for HLA-DRB1 loci. Three novel alleles were identified and officially assigned by the World Health Organization (WHO) Nomenclature Committee as A*0253N, A*1114 and B*5610.


CONCLUSIONS
Large-scale DNA-based HLA genotyping used in bone marrow registry donors is highly accurate and reliable for estimating gene frequency and searching for new alleles. The discrepancy of HLA gene distribution between South and North China Han population showed the necessity of setting the more regions in South and North China to screen the bone marrow registry donors for bone marrow transplant.
Wu
Guo-guang
GG
Shenzhen Institute of Transfusion Medicine, Shenzhen 518035, China.
Deng
Zhi-hui
ZH
Gao
Su-qing
SQ
Cheng
Liang-hong
LH
Jin
Shi-zheng
SZ
Zhou
Dan
D
Li
Zhen
Z
Zou
Hong-yan
HY
Zhang
Xuan
X
Wei
Tian-li
TL
Cheng
Xi
X
Wang
Da-ming
DM
D016026:Bone Marrow Transplantation / Q000706:statistics & numerical data*; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D012042:Registries; D014019:Tissue Donors
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II