HCO3- secretion in the esophageal submucosal glands.
The mammalian esophagus has the capacity to secrete a HCO(3)(-) and mucin-rich fluid in the esophageal lumen. These secretions originate from the submucosal glands (SMG) and can contribute to esophageal protection against refluxed gastric acid. The cellular mechanisms by which glandular cells achieve these secretions are largely unknown. To study this phenomenon, we used the pH-stat technique to measure luminal alkali secretion in an isolated, perfused pig esophagus preparation. Immunohistochemistry was used to localize receptors and transporters involved in HCO(3)(-) transport. The SMG-bearing esophagus was found to have significant basal alkali secretion, predominantly HCO(3)(-), which averaged 0.21 +/- 0.04 microeq.h(-1).cm(-2). This basal secretion was doubled when stimulated by carbachol but abolished by HCO(3)(-) or Cl(-) removal. Basal- and carbachol-stimulated secretions were also blocked by serosal application of atropine, pirenzipine, DIDS, methazolamide, and ethoxzolamide. The membrane-impermeable carbonic anhydrase inhibitor benzolamide, applied to the serosal bath, partially inhibited basal HCO(3)(-) secretion and blocked the stimulation by carbachol. Immunohistochemistry using antibodies to M(1) cholinergic receptor or carbonic anhydrase-II enzyme showed intense labeling of duct cells and serous demilunes but no labeling of mucous cells. Labeling with an antibody to Na(+)-(HCO(3)(-))(n) (rat kidney NBC) was positive in ducts and serous cells, whereas labeling for Cl(-)/HCO(3)(-) exchanger (AE2) was positive in duct cells but less pronounced in serous cells. These data indicate that duct cells and serous demilunes of SMG play a role in HCO(3)(-) secretion, a process that involves M(1) cholinergic receptor stimulation. HCO(3)(-) transport in these cells is dependent on cytosolic and serosal membrane-bound carbonic anhydrase. HCO(3)(-) secretion is also dependent on serosal Cl(-) and is mediated by DIDS-sensitive transporters, possibly NBC and AE2.
Abdulnour-Nakhoul
Solange
S
The VA Medical Center, Attn: Research, Rm. 5F151, 1601 Perdido St., New Orleans, LA 70112-2699, USA. solange@tulane.edu
Nakhoul
Nazih L
NL
Wheeler
Scott A
SA
Wang
Paul
P
Swenson
Eric R
ER
Orlando
Roy C
RC
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D027321:Anion Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism*; D001692:Biological Transport; D002256:Carbonic Anhydrases / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D018679:Cholinergic Agonists / Q000494:pharmacology; D004947:Esophagus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D009092:Mucous Membrane; D043643:Receptor, Muscarinic M1 / Q000378:metabolism; D012704:Serous Membrane / Q000378:metabolism; D013552:Swine
D027321:Anion Transport Proteins; D001639:Bicarbonates; D002712:Chlorides; D018679:Cholinergic Agonists; D043643:Receptor, Muscarinic M1; D002256:Carbonic Anhydrases; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid