title: Allozyme genotypes and tolerance to copper stress in Hediste diversicolor (Polychaeta: Nereididae).
abstract: This study analysed the occurrence of genotypic shifts in laboratory populations of Hediste diversicolor (Polychaeta: Nereididae) exposed to copper stress. Specimens of H. diversicolor were collected at three sites, up to 10 km apart, in the estuarine area of the Pialassa lagoons (North Adriatic Sea, Italy) and were used in acute toxicity tests. Specimens were assigned to copper exposure (0.34 mg/l Cu2+) or control conditions. Each combination of Treatment and Site was replicated in two tanks containing 35 specimens of H. diversicolor. The genotypic structure of both dead and survived specimens was analysed by allozyme electrophoresis at six loci (ALD, FH, HBDH LDH, PGI, SDH). Under copper exposure, specimens with the genotypes ALD100/100 and PGI102/102 had significantly lower mortalities than other genotypes Results were consistent across the three sites, suggesting that, under laboratory conditions, effects of copper stress on H. diversicolor is related to individual genotypes at ALD and PGI loci.
authors.affiliation: Centro Interdipartimentale di Ricerca per le Scienze Ambientali in Ravenna and Dipartimento di Biologia Evoluzionistica e Sperimentale-University of Bologna, Via S. Alberto 163, I-48100, Ravenna, Italy. mvirgilio@ambra.unibo.it
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Virgilio
authors.forename: Marco
authors.initials: M
authors.lastname: Abbiati
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000704:Analysis of Variance; D000818:Animals; D003300:Copper / Q000633:toxicity*; D005838:Genotype; D007527:Isoenzymes; D008522:Mediterranean Sea; D009026:Mortality; D011077:Polychaeta / Q000187:drug effects* / Q000235:genetics*; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D007527:Isoenzymes; D014874:Water Pollutants, Chemical; D003300:Copper