Insights from genomic microarrays into structural chromosome rearrangements.
Array-based comparative genomic hybridization allows high-resolution screening of copy number abnormalities in the genome, and becomes an increasingly important tool to detect deletions and duplications in tumor and post-natal cytogenetics. Here we illustrate that genomic arrays can also provide novel clues regarding the structural basis of chromosome rearrangement, including instability and mechanisms of formation of ring chromosomes. We also showed that array results might impact the recurrence risks for relatives of affected individuals. Our data indicate that chromosome rearrangements frequently involve more breaks than current cytogenetic models assume.
Knijnenburg
Jeroen
J
Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Szuhai
Károly
K
Giltay
Jacques
J
Molenaar
Lia
L
Sloos
Willem
W
Poot
Martin
M
Tanke
Hans J
HJ
Rosenberg
Carla
C
D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D019457:Chromosome Breakage; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015203:Reproducibility of Results; D012303:Ring Chromosomes