Study of mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering.
Langmuir-Blodgett (LB) technique appears to be quite suitable for generating biospecific surfaces and it has potential application for fabricating biosensors. In this work, mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile have been transferred onto hydrophobic silver surface previously modified by 1-octadecanethiol (ODT) SAMs. In order to obtain stable LB films, the influences of different parameters - type of amphiphile, surface pressure and pH - on the properties of mixed IgG/amphiphile monolayer, were investigated. Electrochemical properties of the engineered immunosensor have been measured by impedimetric spectroscopy. The immunosensor obtained exhibits a high sensitivity and a good specificity in a linear dynamic range from 200 to 1000 ng ml(-1).
Hou
Yanxia
Y
IFoS, UMR CNRS, 5621 Ecole Centrale de Lyon, BP 163, 69131 Ecully Cedex, France.
Tlili
Chaker
C
Jaffrezic-Renault
Nicole
N
Zhang
Aidong
A
Martelet
Claude
C
Ponsonnet
Laurence
L
Errachid
Abdelhamid
A
Samitier
Josep
J
Bausells
Joan
J
D000818:Animals; D000941:Antigens / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D008567:Membranes, Artificial; D011817:Rabbits
D000941:Antigens; D020099:Coated Materials, Biocompatible; D007074:Immunoglobulin G; D008567:Membranes, Artificial