[Simultaneous Fluorescent Imaging of Mg2+ and Ca2+].
Simultaneous fluorescent imaging technique with several indicators is a powerful bio-imaging tool for a comprehensive survey about molecular functions in living cells. There are several interactions between Mg(2+) and Ca(2+) such as a counter transport or a competition at binding sites of divalent cation. To measure Mg-Ca images simultaneously, indicators with high selectivity to each divalent cation and multi-color fluorescent imaging system are essential. Here we introduce a setup for Mg-Ca fluorescent imaging we construct, and some reports about Mg-Ca imaging.
Kubota
Takeshi
T
School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University.
Oka
Kotaro
K
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002468:Cell Physiological Phenomena; D002477:Cells / Q000378:metabolism; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008274:Magnesium / Q000378:metabolism / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*
D005456:Fluorescent Dyes; D008274:Magnesium; D002118:Calcium