A novel method for simultaneous purification of albumin and immunoglobulin G.
A novel method was developed to obtain both highly purified bovine serum albumin (BSA) and immunoglobulin G (IgG), at the same time, on a pilot scale. Heat-isopropyl alcohol was used to denature and precipitate the other plasma proteins, except for BSA and IgG; then, CM-Trisacryl was applied to further purify and isolate BSA and IgG. The new procedure produced highly purified BSA and IgG, 98% and 96.8%, respectively, and yielded ideal output, 2.18% and 0.54%, from starting plasma, respectively. The new technique is a rapid and is an available pilot process to prepare the plasma fractions devoid of cellular components.
Xu
Youqing
Y
Science Center, Shantou University, Guangdong, P.R. China.
Ding
Zhaokun
Z
D019840:2-Propanol / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005590:Fractional Precipitation; D006358:Hot Temperature; D007074:Immunoglobulin G / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D010865:Pilot Projects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification*
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D019840:2-Propanol