title: Chemical characterization of sialyl oligosaccharides in milk of the Japanese black bear, Ursus thibetanus japonicus.
abstract: Sialyl oligosaccharides were separated from two samples of Japanese black bear milk by extraction with chloroform/methanol, gel filtration on Bio Gel P-2, ion exchange chromatography on DEAE-Sephadex A-50 and high-performance liquid chromatography (HPLC) on a TSK gel Amido-80 column. They were characterized by (1)H-NMR spectroscopy. The structures of four sialyl oligosaccharides separated from the milk were the following: Neu5Ac(alpha2-3)Gal(beta1-4)Glc. Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-3)[Gal(alpha1-3)Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-6)]Gal(beta1-4)Glc. Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-3)[Gal(alpha1-3)[Fuc(alpha1-2)]Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-6)]Gal(beta1-4)Glc. Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-3)[Neu5Ac(alpha2-6)Gal(beta1-4)GlcNAc(beta1-6)Gal(beta1-4)Glc.
authors.affiliation: Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080-8555, Japan. urashima@obihiro.ac.jp
authors.forename: Tadasu
authors.initials: T
authors.lastname: Urashima
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kumi
authors.initials: K
authors.lastname: Teramoto
authors.forename: Ikichi
authors.initials: I
authors.lastname: Arai
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Komatsu
authors.forename: Toshio
authors.initials: T
authors.lastname: Tsubota
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005260:Female; D007774:Lactation / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry*; D001503:Ursidae / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D012794:Sialic Acids