title: A new approach for the assessment of acrylamide toxicity using a green paramecium.
abstract: Exposure to acrylamide induces neurotoxic effects in humans. In addition, it induces genotoxic, reproductive and carcinogenic effects in laboratory animals. However, no convenient bioassay system for assessing acrylamide toxicity to animal and plant cells has been proposed to date. The present study aims to evaluate acrylamide toxicity to a green paramecium, Paramecium bursaria, bearing many endosymbiotic algae, because some chemicals are highly toxic to paramecia or microalgae, and some protozoa are already used for evaluation of environmental contaminations. Results showed that high acrylamide concentrations (> or = 1500 mg/l) have a lethal effect on P. bursaria. Although low acrylamide concentrations (< or = 150 mg/l) induced less change on the paramecium growth, the number of endosymbiotically growing algal cells drastically decreased. The acrylamide concentration required to induce a 50% decrease in the cell number (IC(50)) was determined to be 7.8 mg/l for endosymbiotic algae, indicating that the algal sensitivity to acrylamide was 7 and 15 times higher than that of Syrian hamster embryo (SHE) cells and the host cells, respectively. Here, we propose the use of P. bursaria being a convenient and sensitive bioindicator as a new approach for the assessment of acrylamide toxicity.
authors.affiliation: Faculty of Science, Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takahashi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yoshii
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kawano
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kosaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosoya
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000826:Animal Testing Alternatives; D000818:Animals; D002452:Cell Count; D002734:Chlorophyll / Q000378:metabolism; D006224:Cricetinae; D048028:DNA, Algal / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D056890:Eukaryota / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008647:Mesocricetus; D010247:Paramecium / Q000187:drug effects* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D013559:Symbiosis; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D048028:DNA, Algal; D002734:Chlorophyll; D020106:Acrylamide