title: Membrane-proximal region of the carboxyl terminus of the gonadotropin-releasing hormone receptor (GnRHR) confers differential signal transduction between mammalian and nonmammalian GnRHRs.
abstract: Recently, we demonstrated that the mammalian type-I GnRH receptor (GnRHR) has a high preference for the phospholipase C/protein kinase C (PLC/PKC)-linked signaling pathway, whereas non-mammalian bullfrog (bf) GnRHRs couple to both adenylate cyclase/protein kinase A (AC/PKA)- and PLC/PKC-linked signaling pathways. In the pre-sent study, using AC/PKA-specific reporter (cAMP-responsive element-luciferase) and PLC/PKC-specific reporter (serum-responsive element-luciferase) systems, we attempted to identify the motif responsible for this difference. A deletion of the intracellular carboxyl-terminal tail (C tail) of bfGnRHR-1 remarkably decreased its ability to induce the AC/PKA-linked signaling pathway. Further dissection of the C tail indicated that an HFRK motif in the membrane-proximal sequence of bfGnRHR-1 C tail is a minimal requirement for the AC/PKA-linked signaling pathway as the addition of this motif to rat GnRHR or deletion of it from bfGnRHR-1 significantly affected the ability to induce the AC/PKA-linked signaling pathway. Deletion or addition of the HFRK motif, however, did not critically influence the PLC/PKC-linked signaling pathway. These results indicate that the HFRK motif in the membrane-proximal region confers the differential signal transduction pathways between mammalian and nonmammalian GnRHRs.
authors.affiliation: Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: D Y
authors.initials: DY
authors.lastname: Oh
authors.forename: Da Young
authors.initials: DY
authors.lastname: Oh
authors.forename: Jung Ah
authors.initials: JA
authors.lastname: Song
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Moon
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Moon
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Kim
authors.forename: Hyuk Bang
authors.initials: HB
authors.lastname: Kwon
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Seong
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011892:Rana catesbeiana; D051381:Rats; D011966:Receptors, LHRH / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D014162:Transfection; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D011966:Receptors, LHRH; D000242:Cyclic AMP; D008156:Luciferases; D011493:Protein Kinase C; D010738:Type C Phospholipases