title: Epigenetic regulation of the binding activity of translation inhibitory proteins that bind the 3' untranslated region of beta-F1-ATPase mRNA by adenine nucleotides and the redox state.
abstract: Here, we describe the binding affinities and the regulation of the binding activities of the fetal liver proteins that interact with the 3' untranslated region of beta-F1-ATPase mRNA (beta-mRNA). These proteins (3'beta FBPs), which are involved in the repression of beta-mRNA translation during fetal development, have poly(A)-binding activity. Reducing agents do not affect the RNA-binding activity of 3'beta FBPs. In contrast, oxidizing and alkylating reagents abolished the binding activity of 3'beta FBPs to its target RNA element, an effect that is partially prevented by the presence of reducing agents. Interestingly, the availability of adenine nucleotides regulates in a concentration-dependent manner the binding activities of 3'beta FBPs. The results suggest that epigenetic changes that occur at the time of birth affecting both the redox and energy state of the liver play a relevant role in the regulation of the binding activities of 3'beta FBPs and therefore in the translation of beta-mRNA.
authors.affiliation: Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, C.S.I.C.-U.A.M., Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Izquierdo
authors.forename: José M
authors.initials: JM
authors.lastname: Cuezva
mesh_terms: D020413:3' Untranslated Regions; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D003432:Cross-Linking Reagents / Q000528:radiation effects; D044127:Epigenesis, Genetic; D005260:Female; D005333:Fetus; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology; D008954:Models, Biological; D010084:Oxidation-Reduction; D011247:Pregnancy; D011485:Protein Binding; D014176:Protein Biosynthesis; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014466:Ultraviolet Rays
chemical_list: D020413:3' Untranslated Regions; D000227:Adenine Nucleotides; D003432:Cross-Linking Reagents; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D006180:Proton-Translocating ATPases