title: Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis.
abstract: The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an alpha(1,2/3)-glucosyltransferase whereas HP1416 encodes an alpha(1,2/4)-glucosyltransferase.
authors.affiliation: Department of Microbiology, National University of Ireland, Galway, Ireland.
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Moran
authors.forename: Bethlehem
authors.initials: B
authors.lastname: Shiberu
authors.forename: John A
authors.initials: JA
authors.lastname: Ferris
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Andreij V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Per-Erik
authors.initials: PE
authors.lastname: Jansson
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
mesh_terms: D005798:Genes, Bacterial / Q000502:physiology*; D005964:Glucosyltransferases / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D005964:Glucosyltransferases