Identification, distribution pattern of IS231 elements in Bacillus thuringiensis and their phylogenetic analysis.
In order to better understand the fundamental biology of Bacillus thuringiensis, a single oligonucleotide primer (5'-CATSSCCATCAASYTAAVR-3') was used to investigate the distribution pattern of IS231 elements in B. thuringiensis by PCR. The results indicated that IS231 elements appeared in 20 standard strains and 107 of 111 China isolates. Three novel IS231, IS231J, IS231O and IS231Q, five variants and a mobile insertion cassette MICBth4 were cloned from eight standard strains of B. thuringiensis, respectively. Interestingly, BLAST analysis revealed that the 5' end of novel IS231J shared 99% identity in 495-bp with a DNA segment adjacent to the 3' end of B. thuringiensis vip1Ac gene (GenBank Accession No. ). Two phylogenetic trees of IS231 elements were constructed and analyzed by neighbor-joining and UPGMA methods from PHYLIP 3.6b program, respectively.
Huang
Tianpei
T
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100094 Beijing, China.
Liu
Jingjing
J
Song
Fuping
F
Shu
Changlong
C
Qiu
Junzhi
J
Guan
Xiong
X
Huang
Dafang
D
Zhang
Jie
J
D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics*; D004251:DNA Transposable Elements; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D004251:DNA Transposable Elements