Genome assembly and particle maturation of the birnavirus infectious pancreatic necrosis virus.
In this study, we have analyzed the morphogenesis of the birnavirus infectious pancreatic necrosis virus throughout the infective cycle in CHSE-214 cells by using a native agarose electrophoresis system. Two types of viral particles (designated A and B) were identified, isolated, and characterized both molecularly and biologically. Together, our results are consistent with a model of morphogenesis in which the genomic double-stranded RNA is immediately assembled, after synthesis, into a large (66-nm diameter) and uninfectious particle A, where the capsid is composed of both mature and immature viral polypeptides. Upon maturation, particles A yield particles B through the proteolytic cleavage of most of the remaining viral precursors within the capsid, the compaction of the particle (60-nm diameter), and the acquisition of infectivity. These studies will provide the foundation for further analyses of birnavirus particle assembly and RNA replication.
Villanueva
Rodrigo A
RA
Laboratorio de Virología, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile. rodrigo.villanueva@ibl.fr.
Galaz
José L
JL
Valdés
Juan A
JA
Jashés
Matilde M
MM
Sandino
Ana María
AM
D000818:Animals; D002478:Cells, Cultured; D016679:Genome, Viral; D018061:Infectious pancreatic necrosis virus / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity*; D009024:Morphogenesis; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012474:Salmon / Q000821:virology; D014771:Virion / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012367:RNA, Viral