title: A flexible data analysis tool for chemical genetic screens.
abstract: High-throughput assays generate immense quantities of data that require sophisticated data analysis tools. We have created a freely available software tool, SLIMS (Small Laboratory Information Management System), for chemical genetics which facilitates the collection and analysis of large-scale chemical screening data. Compound structures, physical locations, and raw data can be loaded into SLIMS. Raw data from high-throughput assays are normalized using flexible analysis protocols, and systematic spatial errors are automatically identified and corrected. Various computational analyses are performed on tested compounds, and dilution-series data are processed using standard or user-defined algorithms. Finally, published literature associated with active compounds is automatically retrieved from Medline and processed to yield potential mechanisms of actions. SLIMS provides a framework for analyzing high-throughput assay data both as a laboratory information management system and as a platform for experimental analysis.
authors.affiliation: Department of Biological Sciences, Columbia University, Fairchild Center, MC 2406, 1212 Amsterdam Avenue, New York, NY 10027 USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Kelley
authors.forename: Mitchell R
authors.initials: MR
authors.lastname: Lunn
authors.forename: David E
authors.initials: DE
authors.lastname: Root
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Flaherty
authors.forename: Allison M
authors.initials: AM
authors.lastname: Martino
authors.forename: Brent R
authors.initials: BR
authors.lastname: Stockwell
mesh_terms: D017362:Cyclic AMP Response Element-Binding Protein; D030541:Databases, Genetic; D004353:Drug Evaluation, Preclinical; D047309:Flavones; D005419:Flavonoids / Q000494:pharmacology; D017930:Genes, Reporter; D006801:Humans; D009134:Muscular Atrophy, Spinal / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D055532:SMN Complex Proteins; D012984:Software
chemical_list: C485383:6,7-dihydroxyflavone; D017362:Cyclic AMP Response Element-Binding Protein; D047309:Flavones; D005419:Flavonoids; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D055532:SMN Complex Proteins