Improved contact lens electrode for corneal ERG recordings in mice.
The electroretinogram (ERG) is routinely used to study retinal physiology in the clinic and in research. Due to their outstanding properties, contact lens electrodes (CLEs) are widely used for ERG recordings. Though the procedures for ERG recordings in mice are similar to those used in humans and larger vertebrates, use of CLEs in the mouse has been limited because of difficulties involved with the manufacturing of small contact lenses. We describe a simple instrument and method for manufacturing contact lenses and CLEs for stable ERG recordings in mice. The instrument operates like a hole-punch and is based on slip joint pliers incorporating a ball bearing on one jaw and forming plate on the other. These CLEs are simple to manufacture, inexpensive and provide stable, long-term recordings of corneal ERGs in mice. With minor modifications, these CLEs could be made for other small animals such as rats or fish.
Sagdullaev
Botir T
BT
Department of Psychological and Brain Sciences, University of Louisville, Louisville, KY 40292, USA. sagdullaev@vision.wustl.edu
DeMarco
Paul J
PJ
McCall
Maureen A
MA
D000818:Animals; D003261:Contact Lenses; D003315:Cornea / Q000502:physiology*; D004596:Electroretinography / Q000295:instrumentation*; D051379:Mice; D008839:Microelectrodes