[Human papillomavirus detection by the hybrid capture II assay with the liquid cell preservation solution Easyfix].
The aim of this study is to validate the liquid cell preservation solution Easyfix for DNA detection of the HPV oncogene using the Hybrid Capture II method. 256 specimens were selected for the cytological study, possible biopsy and HPV oncogene search with the Easyfix fixative fluid and the Cervical Sampler transport medium. The results obtained with both mediums are comparable regardless of the cytological type. The relevance of a cytological study combined with the HPV search is stressed. To conclude, it is possible to put forward that the liquid cell preservation solution Easyfix can be used to detect the HPV oncogene using the Hybrid Capture II method.
Leduc
F
F
Centre Nordpathologie, Lille. npatho.leduc@wanadoo.fr
Bonnière
M
M
Farre
I
I
Staelen
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002584:Cervix Uteri / Q000737:chemistry*; D003470:Culture Media; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D012680:Sensitivity and Specificity; D014626:Vaginal Smears / Q000379:methods*
D003470:Culture Media; D004279:DNA, Viral