title: Molecular and prognostic distinction between serous ovarian carcinomas of varying grade and malignant potential.
abstract: Profiles of gene transcription have begun to delineate the molecular basis of ovarian cancer, including distinctions between carcinomas of differing histology, tumor progression and patient outcome. However, the similarities and differences among the most commonly diagnosed noninvasive borderline (low malignant potential, LMP) lesions and invasive serous carcinomas of varying grade (G1, G2 and G3) have not yet been explored. Here, we used oligonucleotide arrays to profile the expression of 12,500 genes in a series of 57 predominantly stage III serous ovarian adenocarcinomas from 52 patients, eight with borderline tumors and 44 with adenocarcinomas of varying grade. Unsupervised and supervised analyses showed that LMP lesions were distinct from high-grade serous adenocarcinomas, as might be expected; however, well-differentiated (G1) invasive adenocarcinomas showed a strikingly similar profile to LMP tumors as compared to cancers with moderate (G2) or poor (G3) cellular differentiation, which were also highly similar. Comparative genomic hybridization of an independent cohort of five LMP and 63 invasive carcinomas of varying grade demonstrated LMP and G1 were again similar, exhibiting significantly less chromosomal aberration than G2/G3 carcinomas. A majority of LMP and G1 tumors were characterized by high levels of p21/WAF1, with concomitant expression of cell growth suppressors, gadd34 and BTG-2. In contrast, G2/G3 cancers were characterized by the expression of genes associated with the cell cycle and by STAT-1-, STAT-3/JAK-1/2-induced gene expression. The distinction between the LMP-G1 and G2-G3 groups of tumors was highly correlated to patient outcome (chi(2) for equivalence of death rates=7.681189; P=0.0056, log-rank test). Our results are consistent with the recent demonstration of a poor differentiation molecular 'meta-signature' in human cancer, and underscore a number of cell-cycle- and STAT-associated targets that may prove useful as points of therapeutic intervention for those patients with aggressive disease.
authors.affiliation: Department of Gynecology and Obstetrics, University Hospital of Schleswig-Holstein, Kiel, Germany.
authors.forename: Ivo
authors.initials: I
authors.lastname: Meinhold-Heerlein
authors.forename: Dirk
authors.initials: D
authors.lastname: Bauerschlag
authors.forename: Felix
authors.initials: F
authors.lastname: Hilpert
authors.forename: Petre
authors.initials: P
authors.lastname: Dimitrov
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Sapinoso
authors.forename: Marzenna
authors.initials: M
authors.lastname: Orlowska-Volk
authors.forename: Thomas
authors.initials: T
authors.lastname: Bauknecht
authors.forename: Tjoung-Won
authors.initials: TW
authors.lastname: Park
authors.forename: Walter
authors.initials: W
authors.lastname: Jonat
authors.forename: Anja
authors.initials: A
authors.lastname: Jacobsen
authors.forename: Jalid
authors.initials: J
authors.lastname: Sehouli
authors.forename: Jutta
authors.initials: J
authors.lastname: Luttges
authors.forename: Maryla
authors.initials: M
authors.lastname: Krajewski
authors.forename: Stan
authors.initials: S
authors.lastname: Krajewski
authors.forename: John C
authors.initials: JC
authors.lastname: Reed
authors.forename: Norbert
authors.initials: N
authors.lastname: Arnold
authors.forename: Garret M
authors.initials: GM
authors.lastname: Hampton
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; D015398:Signal Transduction; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators