Covalent capture: a natural complement to self-assembly.
The utility of peptide self-assembly can be extended by covalent capture of these supramolecular materials. Disulfide bond formation, native chemical ligation, olefin metathesis, radical capture and oxidative lysine cross-linking have been used recently to help stabilize and characterize a variety of self-assembled peptides. These include natural peptides, proteins and protein mimics such as alpha-helical coiled coils, amyloid-like beta-sheet fibres, portions of p53, glutathione S-transferase and elastin as well as unnatural peptide constructs such as cyclic peptide nanotubes and cylindrical micelles of peptide amphiphiles.
Hartgerink
Jeffrey D
JD
Rice University, Department of Chemistry and Bioengineering, 6100 Main Street, MS60, Houston, TX 77005, USA. jdh@rice.edu
D003641:Deamination; D004220:Disulfides / Q000737:chemistry; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding
D004220:Disulfides; D010455:Peptides