Developmental variability during early embryonic development of zebra fish, Danio rerio.
Early vertebrate embryos pass through a period of remarkable morphological similarity. Possible causes for such similarity of early embryos include modularity, developmental constraints, stabilizing selection, canalization, and exhausted genetic variability. Supposedly, each process creates different patterns of variation and covariation of embryonic traits. We study the patterns of variation of the embryonic phenotype to test ideas about possible evolutionary mechanisms shaping the early embryonic development. We use the zebra fish, Danio rerio, as a model organism and apply repeated measures of individual embryos to study temporal changes of phenotypic variability during development. In particular, we are looking at the embryonic development from 12 hours post fertilization until 27 hours post fertilization. During this time period, the development of individual embryos is documented at hourly intervals. We measured maximum diameter of the eye, length of embryo, number of somites, inclination of somites, and the yolk size (as a maternal effect). The coefficient of variation (CV) was used as a measure of variability that was independent of size. We used a principal component analysis for analysis of morphological integration. The experimental setup kept environment x genotype interactions constant. Nongenetic parental contributions had no significant effects on interindividual variability. Thus all observed phenotypic variation was based on additive genetic variance and error variance. The average CV declined from 14% to 7.7%. The decline of the CV was in particular expressed during 15-19 h post fertilization and occurred in association with multiple correlations among embryonic traits and a relatively high degree of morphological integration. We suggest that internal constraints determine the patterns of variability during early embryonic development of zebra fish.
Irmler
Ingo
I
Department of Biology II, University of Munich (LMU), D-82152 Planegg-Martinsried, Germany.
Schmidt
Kai
K
Starck
J Matthias
JM
D000704:Analysis of Variance; D000818:Animals; D005075:Biological Evolution; D001699:Biometry; D001837:Body Weights and Measures; D004625:Embryo, Nonmammalian / Q000196:embryology; D005123:Eye / Q000033:anatomy & histology; D014644:Genetic Variation; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D025341:Principal Component Analysis; D019170:Somites / Q000166:cytology; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics