[Determination of gentiopicroside in different morphological types of cultivated Gentiana manshurica population].
OBJECTIVE
To study the correlation between morphological variation and gentiopicroside content in cultivated Gentiana manshurica roots and to investigate the feasibility of breeding new varieties of high effective constituent content.


METHODS
Gentiopicroside was determined in 5 morphological types of cultivated G. manshurica roots by HPLC, which are normal (or wild) type, white-flowered type, thick-rooted type, broad-leaved type I and broad-leaved type II.


RESULTS
Among different types gentiopicroside content is the highest in the roots of thick-rooted type, the contents decrease as following order: normal type, broad-leaved type I white-flowered type and broad-leaved type II, and the gentiopicroside contents in the same type root system are a positive correlation with root ages, as 3-years-age roots > 2-years-age roots > 1-year-age varied with roots.


CONCLUSIONS
The contents of effective constituent vary with the morphological variation in cultivated G. manshurica. It is feasible to breed a new variety with high effective constituent with the morphological character as a selecting index.
Wang
Chen
C
Department of Biology Harbin Normal University, Harbin 150080, China.
Xing
Xiu-fang
XF
Ma
Li-xiang
LX
Guan
Yang
Y
Dai
Shao-jun
SJ
Liu
Ming-yuan
MY
D014644:Genetic Variation; D030012:Gentiana / Q000033:anatomy & histology / Q000737:chemistry* / Q000145:classification; D005960:Glucosides / Q000032:analysis*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D018517:Plant Roots / Q000033:anatomy & histology / Q000737:chemistry; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000737:chemistry* / Q000145:classification; D011714:Pyrans / Q000032:analysis*; D013045:Species Specificity
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C012997:gentiopicroside