title: Different staphylococcal strains elicit different levels of production of T-helper 1-inducing cytokines.
abstract: Cytokine production has been implicated in the pathogenic mechanisms of infections caused by the staphylococci, since these bacteria may act as strong cytokine inducers. To gain deeper insight into the Th1 immune response activated by these bacteria, we have analyzed the interferon (IFN), interleukin-12 (IL-12) and IL-18-inducing activities of different Staphylococcus aureus (S. aureus), S. epidermidis and S. saprophyticus strains in human monocytes and murine bone marrow macrophages. A large majority of the S. aureus strains elicited the simultaneous production of IL-12 p70 and IFN-alpha in the human monocytes, while the S. epidermidis and S. saprophyticus strains induced only a low level of production, if any, of these cytokines. Furthermore, a majority of the S. aureus strains induced significantly higher IL-12 p70 and IL-18 titers in the murine bone marrow macrophages than did the S. epidermidis and S. saprophyticus strains. As IL-12, IL-18 and IFN-alpha stimulate Th1 differentiation synergistically, we suggest that S. aureus strains bias the immune response toward a Th1 phenotype, whereas S. epidermidis and S. saprophyticus strains provide a weaker stimulus for the production of Th1-inducing cytokines, and accordingly possibly elicit a less extensive Th1-associated adaptive immunity.
authors.affiliation: Department of Medical Microbiology, University of Szeged, Dóm tér 10, H-6720 Szeged, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Buzás
authors.forename: Klára
authors.initials: K
authors.lastname: Megyeri
authors.forename: A
authors.initials: A
authors.lastname: Miczák
authors.forename: Andrea
authors.initials: A
authors.lastname: Fekete
authors.forename: M
authors.initials: M
authors.lastname: Degré
authors.forename: Yvette
authors.initials: Y
authors.lastname: Mándi
authors.forename: I
authors.initials: I
authors.lastname: Rosztóczy
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D007372:Interferons / Q000096:biosynthesis / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013210:Staphylococcus / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology*
chemical_list: D016207:Cytokines; D007372:Interferons