Study on the interaction between protein and Eu(III)-chlorotetracycline complex and the determination of protein using the fluorimetric method.
Chlorotetracycline (CTC) can react with europium ions Eu3+, and the complex emits the intrinsic fluorescence of Eu3+. The intensity is greatly enhanced by proteins and this forms the basis of a new fluorimetric method for determination of protein. Further research indicates that under optimum conditions, the enhanced intensity of fluorescence is in proportion to the concentration of proteins, in the range 2.0 x 10(-7)-1.0 x 10(-5) g/mL for bovine serum albumin (BSA) (linear equation, I(f) = 34.35933 + 11.54467 x 10(6)C)(r = 0.99895) and 8.0 x 10(-7)-1.0 x 10(-5) g/mL for human serum albumin (HSA) (linear equation, I(f) = 76.58881 + 5.3569 x 10(6)C) (r = 0.99283). Detection limits (S/N = 3) were 8.9 x 10(-9) g/mL for BSA and 3.3 x 10(-8) g/mL for HSA. In an assay for BSA in calf serum, this method gave a value close to that determined by the UV spectrophotometric method.
Liu
Shufang
S
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Yang
Jinghe
J
Wu
Xia
X
Wang
Fei
F
Wang
Feng
F
Jia
Zhen
Z
Mao
Lijuan
L
D002021:Buffers; D002751:Chlortetracycline / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011506:Proteins / Q000737:chemistry*; D012015:Reference Standards; D012710:Serum Albumin, Bovine / Q000032:analysis; D012996:Solutions; D013050:Spectrometry, Fluorescence
D002021:Buffers; D007202:Indicators and Reagents; D011506:Proteins; D012996:Solutions; D012710:Serum Albumin, Bovine; D005063:Europium; D002751:Chlortetracycline