Effects of alkalinization and ATPase inhibition on stromal free Mg2+ concentration in spinach chloroplasts.
Our earlier studies indicate that stromal alkalinization is essential for light-induced increase in free Mg(2+) concentration ([Mg(2+)]) in chloroplast. Stromal [Mg(2+)] was increased by dark incubation of chloroplasts in the K(+)-gluconate medium (pH 8.0), or by NH(4)Cl. These results indicate that stromal alkalinization can induce an increase in stromal [Mg(2+)] without illumination. Some inhibitors of envelope proton-translocating ATPase activity involved in H(+) efflux inhibited the alkalinization-induced increase in [Mg(2+)].
Ishijima
Sumio
S
Graduate School of Agriculture, Kyoto Prefectural University, Japan. ishijima@kpu.ac.jp
Ito
Hiroshi
H
Yoshimura
Hitomi
H
Uchibori
Atsuhito
A
Ohnishi
Masatake
M
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002125:Calcium Gluconate / Q000494:pharmacology; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008027:Light; D008274:Magnesium / Q000378:metabolism*; D018749:RNA, Plant / Q000096:biosynthesis; D018724:Spinacia oleracea / Q000378:metabolism*
D003470:Culture Media; D004791:Enzyme Inhibitors; D018749:RNA, Plant; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D008274:Magnesium; D002125:Calcium Gluconate