Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis.
The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an alpha(1,2/3)-glucosyltransferase whereas HP1416 encodes an alpha(1,2/4)-glucosyltransferase.
Moran
Anthony P
AP
Department of Microbiology, National University of Ireland, Galway, Ireland.
Shiberu
Bethlehem
B
Ferris
John A
JA
Knirel
Yuriy A
YA
Senchenkova
Sof'ya N
SN
Perepelov
Andreij V
AV
Jansson
Per-Erik
PE
Goldberg
Joanna B
JB
D005798:Genes, Bacterial / Q000502:physiology*; D005964:Glucosyltransferases / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry
D008070:Lipopolysaccharides; D005964:Glucosyltransferases