Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients.
In a panel of 18 colon cancer cell lines we found that the thymidylate synthase (TS) genotype was related to TS enzyme activity, but not to TS protein and mRNA levels. In addition, no relation with drug sensitivity was observed. TS genotyping of different tissues from 78 colorectal cancer patients revealed a high level of homology in polymorphic status between normal and malignant tissues and the heterozygous genotype to be the most frequent.
Mauritz
R
R
Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands.
Beumer
I J
IJ
Marsh
S
S
McLeod
H L
HL
Smid
K
K
van Groeningen
C J
CJ
Pinedo
H M
HM
Peters
G J
GJ
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics*; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000494:pharmacology; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D013940:Thymidylate Synthase / Q000096:biosynthesis* / Q000235:genetics*; D014018:Tissue Distribution
D000964:Antimetabolites, Antineoplastic; D012333:RNA, Messenger; D013940:Thymidylate Synthase; D005472:Fluorouracil