title: Colonic inflammation increases Na+ currents in bladder sensory neurons.
abstract: The purpose of this study was to determine whether sensitization of pre-spinal afferents induced by colonic inflammation can affect the physiology of the urinary bladder. Tetrodotoxin-resistant (TTX-R) Na+ currents were examined in bladder sensory neurons after experimental colitis. Cell bodies of bladder dorsal root ganglia were retrogradely labeled and TTX-R Na+ currents were recorded in capsaicin-sensitive neurons. Colitis significantly enhanced response of bladder neurons to capsaicin by approximately 60% and the peak amplitude of TTX-R Na+ current by 51%. These results suggest that colonic inflammation leads to increased excitability of nociceptive bladder neurons.
authors.affiliation: Department of Physiology, University of Oklahoma Health Sciences Center, 940 Stanton L.Young Blvd, Oklahoma City, OK 73104, USA.
authors.forename: Anna P
authors.initials: AP
authors.lastname: Malykhina
authors.forename: Chao
authors.initials: C
authors.lastname: Qin
authors.forename: Robert D
authors.initials: RD
authors.lastname: Foreman
authors.forename: Hamid I
authors.initials: HI
authors.lastname: Akbarali
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D002232:Carbocyanines / Q000378:metabolism; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D016264:Dextran Sulfate; D004347:Drug Interactions; D004558:Electric Stimulation / Q000379:methods; D005727:Ganglia, Spinal / Q000166:cytology*; D015640:Ion Channel Gating / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D001743:Urinary Bladder / Q000166:cytology* / Q000473:pathology / Q000503:physiopathology
chemical_list: C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin; D016264:Dextran Sulfate; D002211:Capsaicin