[Study on the recombinant adeno-associated virus vector carrying LacZ gene expression in the skeletal muscle].
OBJECTIVE
To look for a gene delivery route to the treatment of Duchenne muscular dystrophy(DMD).


METHODS
The recombinant adeno-associated virus vector(rAAV) carrying a LacZ reporter gene was constructed. rAAVLacZ was delivered into the skeletal muscle tissue of C57/BL6 mice by intramuscular injection. Then an intraarterial delivery route was taken to reveal whether rAAVLacZ could transduce muscle tissue.


RESULTS
(1) The LacZ gene was efficiently transduced and expressed persisting for 5 months after intramuscular injection. (2) The membrane of muscle and smooth muscle of vessel was widely transduced by intra-arterial delivery rAAVLacZ.


CONCLUSIONS
These data provide the evidence that rAAVLacZ can efficiently transduce muscle for a long period. Improving intraarterial gene delivery will be promising means for rAAV-mediated gene therapy for generalized skeletal muscle of DMD.
Li
Hong-hua
HH
Department of Neurology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, 430030 P. R. China.
Zhang
Su-ming
SM
Fang
Si-yu
SY
Cheng
Chun-lian
CL
Wang
Dao-wen
DW
Xiao
Xiao
X
D000818:Animals; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics*; D004622:Embryo, Mammalian; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D007273:Injections, Intramuscular; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D007763:Lac Operon / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods
D011994:Recombinant Proteins