title: A comparative evaluation of parasitological, serological and DNA amplification methods for diagnosis of natural Trypanosoma evansi infection in camels.
abstract: A representative number of 217 camels (Camelus dromedarius) from different areas of western Rajasthan State, India, were examined from July 2002 to May 2003 for Trypanosoma evansi infection. The tests used were parasitological (wet blood film, WBF; stained thin blood smear, TBS), immunodiagnostic (double antibody sandwich enzyme linked immunosorbent assay for antigen detection, Ag-ELISA), and DNA amplification by polymerase chain reaction (PCR). These techniques were compared and the best efficiency was found for the last named (PCR). A prevalence of T. evansi infection was detected in 17.05, 9.67, 4.60 and 4.14% by PCR, Ag-ELISA, TBS and WBF with a sensitivity of 100, 56.75, 27.02 and 24.32%, respectively. PCR revealed a specific 227bp band in positive samples. The intensity of PCR bands was variable in different test samples depending upon the level of infection in the test samples. The history of intermittent fever, emaciation, oedema, poor body condition significantly correlated with positive serological status in ELISA as well as trypanosome DNA detection by PCR.
authors.affiliation: Department of Veterinary Parasitology, College of Veterinary and Animal Science, Bikaner 334-001, Rajasthan, India.
authors.forename: Narender
authors.initials: N
authors.lastname: Singh
authors.forename: K M L
authors.initials: KM
authors.lastname: Pathak
authors.forename: Rajender
authors.initials: R
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D002162:Camelus / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D005784:Gene Amplification; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D007194:India / Q000453:epidemiology; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015995:Prevalence; D011817:Rabbits; D012680:Sensitivity and Specificity; D014345:Trypanosoma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D007106:Immune Sera