title: [BAY11-7082 and Lactacystein in CD154-induced NF-kappaB activation].
abstract: OBJECTIVE
  To investigate the inhibition mechanisms of BAY11-7082 (IkappaB-alpha phosphorylation inhibitor) and Lactacystein (proteosome inhibitor) in CD154-induced NF-kappaB activation.
  
  
  METHODS
  We used recombinant CD154 to stimulate EBV/LMP1 negative Ramos B cell and observed the effects of BAY11-7082 and Lactacystein in CD154-induced NF-kappaB luciferase activation, phosphorylation and degradation of IkappaB-alpha, phosphorylation of p65, and nuclear translocation of NF-kappaB subunits upon CD154 stimulation.
  
  
  RESULTS
  Both BAY11-7082 and Lactacystein abrogated CD154-induced NF-kappaB luciferase activation in Ramos cells. While CD154-induced phosphorylation of p65, phosphorylation and degradation of IkappaB-alpha, and nuclear translocation of p50, p65, and c-Rel were all blocked by BAY11-7082; Lactacystein only inhibited degradation of IkappaB-alpha and p65 nuclear translocation.
  
  
  CONCLUSIONS
  BAY11-7082 and Lactacystein inhibit CD154-induced NF-kappaB activation through different mechanisms.
authors.affiliation: Department of Rheumatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. zxpumch2003@yahoo.com.cn
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Zeng
authors.forename: Feng-chun
authors.initials: FC
authors.lastname: Zhang
authors.forename: Fu-lin
authors.initials: FL
authors.lastname: Tang
authors.forename: Meng-xue
authors.initials: MX
authors.lastname: Yu
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002051:Burkitt Lymphoma / Q000473:pathology; D023201:CD40 Ligand / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology*; D013450:Sulfones / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D015853:Cysteine Proteinase Inhibitors; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones; C067713:lactacystin; D023201:CD40 Ligand; D000111:Acetylcysteine