Artocarpin is a polyspecific jacalin-related lectin with a monosaccharide preference for mannose.
A reinvestigation of the carbohydrate-binding properties revealed that artocarpin, a previously described mannose-specific lectin from jackfruit (Artocarpus integrifolia) seeds, behaves as a polyspecific lectin. Surface plasmon resonance hapten inhibition experiments demonstrated that artocarpin readily interacted with a wide range of monosaccharides covering galactose, N-acetylgalactosamine, mannose, glucose, sialic acid and N-acetylmuramic acid. Molecular docking confirmed this unexpected ability of artocarpin to interact with structurally different sugars. The biological significance of the polyspecificity of the lectin is discussed in terms of the broadening of the range of potential target glycans present on the surface of plant phytopathogens or predators.
Barre
Annick
A
Surfaces Cellulaires et Signalisation chez les Végétaux, UMR-CNRS 5546, Pôle de Biotechnologie Végétale, 24 chemin de Borde-Rouge, BP 17, Auzeville, 31326 Castanet-Tolosan, France.
Peumans
Willy J
WJ
Rossignol
Michel
M
Borderies
Gisèle
G
Culerrier
Raphaël
R
Van Damme
Els J M
EJ
Rougé
Pierre
P
D031622:Artocarpus / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D037241:Mannose-Binding Lectins / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013379:Substrate Specificity
D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C090683:artocarpin lectin; C043696:jacalin; D008358:Mannose