Proteasome inhibitors induce changes in chromatin structure characteristic of senescent human fibroblasts.
Inhibitors of proteasome induced premature senescence in normal human fibroblasts. Besides morphological alteration and expression of senescence marker genes, these cells manifested senescence-associated heterochromatic foci under staining of the nuclei with DAPI similar to normally senescent cells. These results suggest that declining ability in protein degradation may be involved in the formation of heterochromatic foci in senescent fibroblasts.
Ukekawa
Ryo
R
Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Japan.
Maegawa
Naoyuki
N
Mizutani
Emi
E
Fujii
Michihiko
M
Ayusawa
Dai
D
D000375:Aging / Q000502:physiology; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002843:Chromatin / Q000737:chemistry* / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure*; D006801:Humans; D061988:Proteasome Inhibitors
D002843:Chromatin; D061988:Proteasome Inhibitors