Efficient cleavage of DS DNA by bleomycin conjugated via hexaethylene glycol linker to triplex-forming oligonucleotides.
New conjugates of bleomycin A5 with oligonucleotides are synthesized. The bleomycin residue was attached to the 3'- or 5'- terminus of hexadecathymidilate via a hexaethylene glycol phosphate linker. Newly designed conjugates were shown to cleave site-specifically both strands of a dsDNA fragment within a triplex. The maximum extent of cleavage for individual strand amounts to 61%.
Vorobjev
Pavel
P
Institute of Chemical Biology and Fundamental Medicine SD RAS, Novosibirsk, Russia.
Tchaika
Olessia
O
Zarytova
Valentina
V
D001345:Autoradiography; D001483:Base Sequence; D001761:Bleomycin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005026:Ethylene Glycols / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
D005026:Ethylene Glycols; D009841:Oligonucleotides; D001761:Bleomycin; C077067:hexaethylene glycol; D004247:DNA