title: Oriented attachment and membrane reconstitution of His-tagged cytochrome c oxidase to a gold electrode: in situ monitoring by surface-enhanced infrared absorption spectroscopy.
abstract: A novel concept is introduced for the oriented incorporation of membrane proteins into solid supported lipid bilayers. Recombinant cytochrome c oxidase solubilized in detergent was immobilized on a chemically modified gold surface via the affinity of its histidine-tag to a nickel-chelating nitrilo-triacetic acid (NTA) surface. The oriented protein monolayer was reconstituted into the lipid environment by detergent substitution. The individual steps of the surface modification, including (1) chemical modification of the gold support, (2) adsorption of the protein, and (3) reconstitution of the lipid bilayer, were followed in situ by means of surface-enhanced infrared absorption spectroscopy (SEIRAS) and accompanied by normal-mode analysis. The high surface sensitivity of SEIRAS allows for the identification of each chemical reaction process within the monolayer at the molecular level. Finally, full functionality of the surface-tethered cytochrome c oxidase was demonstrated by cyclic voltammetry after binding of the natural electron donor cytochrome c.
authors.affiliation: Forschungszentrum Jülich, IBI-2: Structural Biology, Jülich, Germany.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ataka
authors.forename: Frank
authors.initials: F
authors.lastname: Giess
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Knoll
authors.forename: Renate
authors.initials: R
authors.lastname: Naumann
authors.forename: Sabina
authors.initials: S
authors.lastname: Haber-Pohlmeier
authors.forename: Björn
authors.initials: B
authors.lastname: Richter
authors.forename: Joachim
authors.initials: J
authors.lastname: Heberle
mesh_terms: D004563:Electrochemistry; D003576:Electron Transport Complex IV / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D008958:Models, Molecular; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D008051:Lipid Bilayers; D011994:Recombinant Proteins; D006639:Histidine; D006046:Gold; D003576:Electron Transport Complex IV; D009571:Nitrilotriacetic Acid