title: Prognostic significance of detecting micrometastases by tyrosinase RT/PCR in sentinel lymph node biopsies: lessons from 322 consecutive melanoma patients.
abstract: This prospective study was performed to determine the prognostic value of tyrosinase mRNA detection in sentinel lymph nodes (SLN) of melanoma patients. About 847 SLNs from 322 consecutive patients were assessed by histopathology and immunohistochemistry as well as tyrosinase-reverse transcriptase-polymerase chain reaction (RT/PCR) for the presence of micrometastases. The results were correlated with the prognostic parameters employing a multivariate analysis after a median follow-up of 37 months. Histopathological analysis revealed metastases in 34/322 patients (10.6%). Among the 288 patients with histopathologically negative SLN, tyrosinase-mRNA was detected in 39 patients. A relapse of the tumour occurred in 44.1% of the patients with histopathologically positive SLN, in 25.6% with histopathologically negative, but tyrosinase-RT/PCR-positive SLN, and 8.0% with "double-negative" SLN. A multivariate analysis identified tumour thickness, the histopathological SLN status, and the ulceration of the primary tumour as independent prognostic factors. Thus, by assessing tyrosinase mRNA in the SLN of melanoma patients, we identified a subgroup with histopathologically negative, but Tyr-RT-PCR-positive SLN who have a high risk of disease relapse.
authors.affiliation: Department of Dermatology and Venereology, Otto-von-Guericke-University, Leipziger Strasse 44, D-39120 Magdeburg, Germany. jens.ulrich@medizin.uni-magdeburg.de
authors.forename: J
authors.initials: J
authors.lastname: Ulrich
authors.forename: B
authors.initials: B
authors.lastname: Bonnekoh
authors.forename: R
authors.initials: R
authors.lastname: Böckelmann
authors.forename: M
authors.initials: M
authors.lastname: Schön
authors.forename: M P
authors.initials: MP
authors.lastname: Schön
authors.forename: R
authors.initials: R
authors.lastname: Steinke
authors.forename: A
authors.initials: A
authors.lastname: Roessner
authors.forename: U
authors.initials: U
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Gollnick
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015331:Cohort Studies; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008545:Melanoma / Q000473:pathology*; D008875:Middle Aged; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D011379:Prognosis; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D021701:Sentinel Lymph Node Biopsy; D012878:Skin Neoplasms / Q000473:pathology*
chemical_list: D014442:Monophenol Monooxygenase