title: Identification of the putative collagen X gene from the pufferfish Fugu rubripes.
abstract: The type X collagen gene is the classical marker of hypertrophic chondrocytes. Mutations in the human collagen X gene cause metaphyseal chondrodysplasia type Schmid (MCDS). In order to gain insight into the evolution of collagen X genes, we identified a putative collagen X gene from the pufferfish Fugu rubripes. We demonstrated expression of the putative Fugu collagen X gene by reverse transcription-polymerase chain reaction (RT-PCR) with primers spanning intron 1. The Fugu collagen X gene shares a common gene structure and high amino acid identity with mammalian and chicken collagen X genes. Interestingly, we have found that most of the residues mutated in human MCDS are highly conserved in the Fugu gene. The availability of the Fugu collagen X gene sequence will be of value in the identification of functionally important residues within the protein and for delineating regulatory elements that control collagen X gene expression in chondrocytes.
authors.affiliation: Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada.
authors.forename: Anita
authors.initials: A
authors.lastname: Woods
authors.forename: Claudine
authors.initials: C
authors.lastname: James
authors.forename: T Michael
authors.initials: TM
authors.lastname: Underhill
authors.forename: Frank
authors.initials: F
authors.lastname: Beier
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D024121:Collagen Type X / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D023741:Takifugu / Q000235:genetics*; D024363:Transcription Initiation Site
chemical_list: D024121:Collagen Type X; D012313:RNA