title: Crystallization and preliminary X-ray crystallographic analysis of a non-specific lipid-transfer protein with antipathogenic activity from Phaseolus mungo.
abstract: A 9 kDa non-specific lipid-transfer protein (nsLTP) from mung bean (Phaseolus mungo) seeds, displaying antifungal activity, antibacterial activity and lipid-transfer activity, was crystallized at 297 K using ammonium sulfate as a precipitant by means of the hanging-drop vapour-diffusion method. Native X-ray diffraction data were collected to a resolution of 2.4 A. The crystals are rhombohedral, belonging to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.671, b = 51.785, c = 55.925 A. Assuming the presence of one molecule in the crystallographic asymmetric unit results in a Matthews coefficient (V(M)) of approximately 3.0 A(3) Da(-1), corresponding to a solvent content of about 58%.
authors.affiliation: Institute of Biotechnology, Fuzhou University, Fujian 350002, People's Republic of China.
authors.forename: Shao Yun
authors.initials: SY
authors.lastname: Wang
authors.forename: Kang Jing
authors.initials: KJ
authors.lastname: Zhou
authors.forename: Xiu Yun
authors.initials: XY
authors.lastname: Ye
authors.forename: Zhi Bin
authors.initials: ZB
authors.lastname: Xu
authors.forename: Jin Hong
authors.initials: JH
authors.lastname: Wu
authors.forename: Ping Fan
authors.initials: PF
authors.lastname: Rao
mesh_terms: D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013045:Species Specificity; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D049294:Phospholipid Transfer Proteins; D010940:Plant Proteins; D000645:Ammonium Sulfate