Micromechanics of tumor cell adhesion and migration under dynamic flow conditions.
Attachment of tumor cells to endothelial cells is critical for migration of tumor cells out of the vascular system to establish metastases. We have studied human melanoma cell (C8161) adhesion and migration in response to stimulation by soluble collagen IV (CIV) using a modified Boyden chamber. In this modified chamber, shear flow can be introduced over the cell-substrate interface affecting tumor cell chemotactic migration through a microporous filter. Our results suggest that transmigration of C8161 cells under flow conditions can be influenced by PMNs, mediated by Mac-1 and ICAM-1 adhesive interactions and enhanced by altered cytokine production.
Dong
Cheng
C
Department of Bioengineering, The Pennsylvania State University, University Park, PA 16802-6804, USA. cxd23@psu.edu
Slattery
Margaret
M
Liang
Shile
S
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000473:pathology*; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D013314:Stress, Mechanical
D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1