Carbohydrate metabolism in the central nervous system of the megalobulimus oblongus snail during anoxia exposure and post-anoxia recovery.
The effects of anoxic exposure and the post-anoxia aerobic recovery period on carbohydrate metabolism in the central nervous system (CNS) of the land snail Megalobulimus oblongus, an anoxia-tolerant land gastropod, were studied. The snails were exposed to anoxia for periods of 1.5, 3, 6, 12, 18, or 24 hr. In order to study the post-anoxia recovery phase, snails exposed to a 3-hr period of anoxia were returned to aerobic conditions for 1.5, 3, 6, or 15 hr. Glycogen and glucose concentrations in the CNS, hemolymph glucose concentration, and glycogen phosphorylase (active form, GPa) activity in the CNS were analyzed. Anoxia does not significantly affect the concentration of CNS glucose but induces hyperglycemia and a reduction of CNS GPa activity. The glycogen concentration was decreased at 12 hr of anoxia; however, by 18 and 24 hr in anoxia, the glycogen content was not significantly different from basal control values. During the post-anoxia period, the reduction in GPa activity and the increased hemolymph glucose concentration induced by anoxia returned to control values. These results suggest that the CNS of M. oblongus may use hemolymph glucose to fulfill the metabolic demands during anoxia. However, the hypothesis of tissue metabolic arrest cannot be excluded.
De Fraga
Luciano S
LS
Departamento de Fisiologia, Laboratório de Neurobiologia Comparada e Laboratório de Metabolismo e Endocrinologia de Invertebrados, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 90050-170, Porto Alegre, RS, Brazil.
Da Silva
Roselis S M
RS
Achaval
Matilde
M
Zancan
Denise M
DM
D000222:Adaptation, Physiological; D000693:Anaerobiosis / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002490:Central Nervous System / Q000378:metabolism* / Q000503:physiopathology; D006003:Glycogen / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism*; D020127:Recovery of Function; D012908:Snails / Q000378:metabolism*
D001786:Blood Glucose; D006003:Glycogen