title: The distribution of CD34-positive stromal cells and myofibroblasts in colorectal carcinoid tumors.
abstract: In order to understand the stromal reaction associated with colorectal neoplasms, we examined specimens from 26 patients including normal colorectal tissues (n=15), carcinoid tumors (n=12), well differentiated adenocarcinomas (n=10), and poorly differentiated adenocarcinomas (n=4), using an immunohistochemical method. Myofibroblasts and CD34-positive stromal cells were distributed in the mucosa and in the area between the submucosal and subserosal layers, respectively. However, the distribution of these cells markedly changed with the invasion of neoplasms. Namely, myofibroblasts were abundant in the invasive stroma of all colorectal neoplasms. CD34-positive stromal cells were completely absent from the invasive stroma of colorectal cancers. On the other hand, CD34-positive stromal cells were absent from four out of five carcinoid tumor cases with lesions measuring less than 2 mm in size, but were present in all seven cases of carcinoid tumors measuring more than 2 mm. Double-immunostaining identified stromal cells expressing both ASMA and CD34 in several carcinoid tumor cases. Finally, no CD34-positive stromal cells were observed in the invasive stroma of colorectal cancers. However, the distribution of these cells in carcinoid tumors may depend on the lesion size. Namely, CD34-positive stromal cells existed between neoplastic nests in large-sized carcinoid tumors. Myofibroblasts in the stroma of colorectal neoplasms may originate from CD34-positive stromal cells.
authors.affiliation: Department of Pathology, Program of Bioregulation and Genetics, Kochi Medical School, Kochi University, Kochi 783-8505, Japan. nkuroda@med.kochi-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Kuroda
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: E
authors.initials: E
authors.lastname: Miyazaki
authors.forename: M
authors.initials: M
authors.lastname: Toi
authors.forename: M
authors.initials: M
authors.lastname: Hiroi
authors.forename: H
authors.initials: H
authors.lastname: Enzan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000276:immunology*; D002276:Carcinoid Tumor / Q000276:immunology*; D003106:Colon / Q000166:cytology / Q000276:immunology; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D017154:Stromal Cells / Q000276:immunology
chemical_list: D018952:Antigens, CD34