title: Functional properties of the alternative NADH:ubiquinone oxidoreductase from E. coli through comparative 3-D modelling.
abstract: The alternative NADH:ubiquinone oxidoreductase (NDH-2) from Escherichia coli is a membrane protein playing a prominent role in respiration by linking the reduction of NADH to the quinone pool. Remote sequence similarity reveals an evolutionary relation between alternative NADH:quinone oxidoreductases and the SCOP-family "FAD/NAD-linked reductases". We have created a structural model for NDH-2 from E. coli through comparative modelling onto a template from this family. Combined analysis of our model and sequence conservation allowed us to include the cofactor FAD and the substrate NADH in atomic detail. Furthermore, we propose the most plausible orientation of NDH-2 relative to the membrane and specify a region of the protein potentially involved in ubiquinone binding.
authors.affiliation: Biocomputing Research Unit, School of Biology, University of Edinburgh, Edinburgh, EH9 3JR, UK.
authors.forename: Ralf
authors.initials: R
authors.lastname: Schmid
authors.forename: Dietlind L
authors.initials: DL
authors.lastname: Gerloff
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D019069:Cell Respiration / Q000502:physiology; D003300:Copper / Q000378:metabolism; D042967:Electron Transport Complex I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D009243:NAD; D014451:Ubiquinone; D005182:Flavin-Adenine Dinucleotide; D003300:Copper; D042967:Electron Transport Complex I