[T-bet gene expression in acute graft versus host disease].
OBJECTIVE
To study the role of T-bet [a T helper 1 (Th1) lymphocyte transcription factor] gene expression in predicting acute graft-versus-host disease (aGVHD) and evaluate the correlation between T-bet gene and aGVHD.


METHODS
Twenty patients who underwent allogeneic stem cell transplantation (allo. HSCT) entered this study. The expression of T-bet gene was examined with reverse transcription (RT)-PCR in bone marrow samples collected from patients on the day before conditioning, and day 0, day 14, day 28, and day 42 after HSCT.


RESULTS
The expression level of T-bet in patients developed aGVHD was increased compared with that before conditioning (P = 0.043). The incidence of aGVHD was 91.7% in patients whose T-bet expression level was increased on day 14 after transplant while was 12.5% in those whose T-bet gene expression level was not increased on that day (P < 0.001).


CONCLUSIONS
Patients with increased expression levels of T-bet after allo-HSCT may have an increased possibility to develop aGVHD. T-bet expression level may serve as an advisable guide to the clinician in predicting aGVHD and monitoring treatment.
Yan
Zhang-song
ZS
Institute of Hematology and Blood Disease Hospital, CAMS and PUMC, Tianjin 300020, China.
Liu
Qing-guo
QG
Yang
Dong-lin
DL
Zhou
Shi-yong
SY
Jiang
Er-lie
EL
Huang
Yong
Y
Wang
He-hua
HH
Zhai
Wen-jing
WJ
Zhou
Zheng
Z
Wang
Mei
M
Feng
Si-zhou
SZ
Han
Ming-zhe
MZ
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000378:metabolism*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020825:T-Box Domain Proteins; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D000098815:T-bet Transcription Factor
D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014157:Transcription Factors