A comparative binding study of BIV Tat peptide against its TAR RNA duplex, RNA-DNA heteroduplex and DNA duplex.
Association between RNA and DNA strands to form RNA-DNA heteroduplex is important in many biological processes such as transcription, DNA replication and reverse transcription. Herein, binding affinities of a 17-mer BIV Tat peptide is compared with TAR DNA duplex, TAR RNA-DNA heteroduplex and TAR RNA duplex. It was observed that binding affinities of Tat peptide is comparable against DNA-RNA heteroduplex and RNA duplex, whereas DNA duplex binding is decidedly poor.
Tok
Jeffrey B-H
JB
Department of Chemistry, York College and Graduate Center, The City University of New York (CUNY), 94-20 Guy R. Brewer Blvd., Jamaica, NY 11451, USA. tok2@llnl.gov
Bi
Lanrong
L
D001483:Base Sequence; D002942:Circular Dichroism; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015696:Gene Products, tat / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes; D011485:Protein Binding; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D015696:Gene Products, tat; D009692:Nucleic Acid Heteroduplexes; D012367:RNA, Viral