High-risk HPV detection by Hybrid Capture and ploidy determination by a computer-assisted system in cervical biopsies.
OBJECTIVE
To correlate high-risk HPV (hrHPV) detection by Hybrid Capture II (HC2) (Digene, Gaithersburg, Maryland, U.S.A.) with DNA content (ploidy) of cervical biopsies analyzed by a computer-assisted system.


METHODS
Cervical biopsies from 54 women examined at Leonor Mendes de Barros Hospital, São Paulo, as part of the Latin American Screening study during 2002--2003, were tested for hrHPV with HC2. All patients had been referred for colposcopic examination due to an abnormal cervical cytology. The final diagnosis included 30 cervicitis, 14 cervical intraepithelial neoplasia (CIN) 1, 5 CIN 2, 4 CIN 3 and 1 squamous cell carcinoma (SCC). Five-micrometer sections of each biopsy were stained with Feulgen-tionine and evaluated with the CAS 200 System (Becton Dickinson, U.S.A.), using the 3.0 software (version 8.1) of the DNA Quantitative Measurement Program (Becton Dickinson). Ploidy was evaluated from histograms obtained by analyzing atypical nuclei.


RESULTS
Of the 30 cervicitis cases, 28 (93.3%) were diploid, and hrHPV was detected in 8 (28.5%) of the cases. Two tetraploid cervicitis lesions were observed, 1 positive and 1 negative for hrHPV. Among the CIN 1 lesions, 8 (57.1%) were diploid and 6 (42.8%) aneuploid. Of the latter, 4 (66.6%) were negative and 2 (33.3%) positive for hrHPV. Of the 5 CIN 2 lesions, 2 were diploid, 2 aneuploid and 1 tetraploid; all were positive for hrHPV. All CIN 3 lesions and the SCC proved to be aneuploid and positive for hrHPV.


CONCLUSIONS
The data suggest that the majority of cervicitis and CIN 1 lesions are diploid and negativef or hrHPV. This is in sharp contrast to high grade CIN 2-3 lesions, all of which were positive for hrHPV in this study and also aneuploid, consistent with their progressive potential.
Shirata
Neuza Kasumi
NK
Instituto Adolfo Lutz, São Paulo, Brazil. shirata86@hotmail.com
Espoladore
Luiz Marcelo Warneck
LM
Filho
Adhemar Longatto
AL
Pittoli
Janaína Erica
JE
di Loreto
Celso
C
Pereira
Sonia Maria Miranda
SM
Maeda
Marina Yoshie Sakamoto
MY
Utagawa
Maria Lúcia
ML
da Silva Aguiar
Luciana
L
Roteli-Martins
Cecília
C
Martinez
Edson G
EG
Syrjänen
Kari
K
D002294:Carcinoma, Squamous Cell; D002467:Cell Nucleus / Q000473:pathology; D002584:Cervix Uteri / Q000473:pathology*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D011003:Ploidies; D012306:Risk; D002575:Uterine Cervicitis / Q000175:diagnosis; D002578:Uterine Cervical Dysplasia