title: Properties of antigenomic hepatitis delta virus ribozyme cis- and trans- analogs.
abstract: A series of permuted variants of antigenomic HDV ribozyme and trans-acting variants were constructed. The catalytic activity study of the ribozymes has shown that all the variants were capable of self-cleaving with equally biphasic kinetics. Ribonuclease and Fe(II)-EDTA cleavage have provided evidence that all designed ribozymes fold according to the pseudoknot model and the conformations of the initial and cleaved ribozyme are different. A scheme of HDV ribozyme self-cleavage reaction was suggested. The role of hydrogen bonds in the reaction was evaluated by substitution of ribose in the ribozyme for deoxyribose. It was found that the 2'-OH group of U23 and C27 is critical for the reaction to occur; the 2'-OH group of U32 and U39 is important, while 2'-OH groups of other nucleotides of loop 3, stem 4 and stem 1 are unimportant for the cleavage activity.
authors.affiliation: Russian Cardiology Research and Development Center, Moscow, Russia. lasav@cardio.ru
authors.forename: L
authors.initials: L
authors.lastname: Savochkina
authors.forename: V
authors.initials: V
authors.lastname: Alekseenkova
authors.forename: T
authors.initials: T
authors.lastname: Belyanko
authors.forename: M
authors.initials: M
authors.lastname: Lukin
authors.forename: R
authors.initials: R
authors.lastname: Beabealashvilli
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D016679:Genome, Viral; D003698:Hepatitis Delta Virus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000737:chemistry*
chemical_list: D016337:RNA, Catalytic