title: Metacercariae of Clinostomum attenuatum in Ambystoma tigrinum mavortium, Bufo cognatus and Spea multiplicata from west Texas.
abstract: Tissues from barred tiger salamanders (Ambystoma tigrinum mavortium), Great Plains toads (Bufo cognatus) and New Mexico spadefoots (Spea multiplicata) collected from 16 playa wetlands in Texas during 1999 and 2000 were examined by light microscopy. Digenean cysts were primarily distributed subcutaneously throughout the specimens and occasionally coelomic invasion was noted. The parasites within the cysts were 1.5-2 mm in diameter, with a thin (c. 10 microm wide) eosinophilic-staining tegument, two suckers (oral and ventral), posteriorly located primordial genitalia and paired digestive caeca. These digeneans were identified as the metacercariae of Clinostomum attenuatum. This is the first record of Clinostomum attenuatum in these amphibian species.
authors.affiliation: Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, 43 Brighton Road, Tifton, GA 31793, USA. dmiller@tifton.edu
authors.forename: D L
authors.initials: DL
authors.lastname: Miller
authors.forename: C R
authors.initials: CR
authors.lastname: Bursey
authors.forename: M J
authors.initials: MJ
authors.lastname: Gray
authors.forename: L M
authors.initials: LM
authors.lastname: Smith
mesh_terms: D000557:Ambystoma / Q000469:parasitology*; D000818:Animals; D002025:Bufonidae / Q000469:parasitology*; D008018:Life Cycle Stages; D011898:Ranidae / Q000469:parasitology*; D013781:Texas; D014200:Trematoda / Q000254:growth & development* / Q000648:ultrastructure