Characterization of an antifungal glycolipid secreted by the yeast Sympodiomycopsis paphiopedili.
An antifungal glycolipid was purified from the culture liquid of the ustilaginomycetous yeast Sympodiomycopsis paphiopedili by column and thin-layer chromatography. According to nuclear magnetic resonance and mass-spectroscopy experiments it was a cellobioside containing 2,15,16-trihydroxypalmitic acid as an aglycon. The minimal effective concentrations leading to ATP leakage and growth inhibition were 45 and 160 microg ml(-1) for Cryptococcus terreus and Candida albicans, respectively.
Kulakovskaya
Tatiana V
TV
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow region, Russia. alla@ibpm.pushchino.ru
Shashkov
Alexander S
AS
Kulakovskaya
Ekaterina V
EV
Golubev
Wladyslav I
WI
D000255:Adenosine Triphosphate / Q000378:metabolism; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D002176:Candida albicans / Q000254:growth & development; D002855:Chromatography, Thin Layer; D003454:Cryptococcus / Q000254:growth & development; D006017:Glycolipids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D021241:Spectrometry, Mass, Electrospray Ionization
D000935:Antifungal Agents; D006017:Glycolipids; D000255:Adenosine Triphosphate