Effects of expression of hemA and hemB genes on production of porphyrin in Propionibacterium freudenreichii.
The genus Propionibacterium has a wide range of probiotic activities that are exploited in dairy and fermentation systems such as cheeses, propionic acid, and tetrapyrrole compounds. In order to improve production of tetrapyrrole compounds, we expressed the hemA gene, which encodes delta-aminolevulinic acid (ALA) synthase from Rhodobacter sphaeroides, and the hemB gene, which encodes porphobilinogen (PBG) synthase from Propionibacterium freudenreichii subsp. shermanii IFO12424, either monocistronically or polycistronically in strain IFO12426. The recombinant strains accumulated larger amounts of ALA and PBG, with resultant 28- to 33-fold-higher production of porphyrinogens, such as uroporphyrinogen and coproporphyrinogen, than those observed in strain IFO12426, which harbored the shuttle vector pPK705.
Piao
Yongzhe
Y
Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-oka 2-1, Suita, Osaka 565-0871, Japan.
Kiatpapan
Pornpimon
P
Yamashita
Mitsuo
M
Murooka
Yoshikatsu
Y
D000624:5-Aminolevulinate Synthetase / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D000623:Porphobilinogen Synthase / Q000235:genetics / Q000378:metabolism*; D011166:Porphyrins / Q000096:biosynthesis*; D011424:Propionibacterium / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016992:Rhodobacter / Q000201:enzymology / Q000235:genetics
D001426:Bacterial Proteins; D011166:Porphyrins; D011994:Recombinant Proteins; D000624:5-Aminolevulinate Synthetase; D000623:Porphobilinogen Synthase