title: [Application of random amplification polymorphic DNA in the genotyping of Neisseria gonorrhoeae].
abstract: OBJECTIVE
  To set up random amplified polymorphic DNAs (RAPD) method in genotyping Neisseria gonorrhoeae on DNA level, and to explore its use to trace the source of infection.
  
  
  METHODS
  Four different pretreatments were used to extract the Neisseria gonorrhoeae genomic DNA with its advantages and disadvantages compared. Arbitrary sequence was then used to amplify the genomic DNA of Neisseria gonorrhoeae and RAPD fingerprint maps was applied to distinct the Neisseria gonorrhoeae strains. Finally, RAPD fingerprint of Neisseria gonorrhoeae strain between patient and his/her sexual partner was compared.
  
  
  RESULTS
  Cetyltrimethylammonium bromide method was classical in extracting genomic DNA, and could get integrated genomic DNA and good fingerprint maps, since main segments were common to all the Neisseria gonorrhoeae but some were different among strains so that the fingerprint of different Neisseria gonorrhoeae were distinctive. However, fingerprint maps of Neisseria gonorrhoeae collected from sex partners were quite similar.
  
  
  CONCLUSIONS
  Based on genomic levels, effective fingerprint maps could be identified and to classify the Neisseria gonorrhoeae into different genotypes. RAPD fingerprint maps could be used to trace the source of infection.
authors.affiliation: School of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Zhang
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Ren
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Zhang
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Zhou
authors.forename: Shun-zhang
authors.initials: SZ
authors.lastname: Yu
authors.forename: Qing-wu
authors.initials: QW
authors.lastname: Jiang
mesh_terms: D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004269:DNA, Bacterial