title: Identification and characterization of an interspersed repetitive DNA fragment in Plasmodium vivax with potential use for specific parasite detection.
abstract: We cloned and characterized a Plasmodium vivax repeat element of 7872bp named PvRE7.8. Several internal tandem repeats were found along the sequence. The repetitive nature of the PvRE7.8 element was confirmed by hybridization of a P. vivax YAC library. Based on the data bank analysis and the presence of two contiguous putative genes that may encode proteins related to DNA metabolism, PvRE7.8 could be considered an inactivated transposon-LINE element. By using Pv79 as probe or primers derived from Pv79-flanking sequences, P. vivax DNA Could be detected from whole blood and mosquito samples. We consider that the repeat element described here has potential for P. vivax malaria diagnosis and for epidemiological analysis of P. vivax transmission areas.
authors.affiliation: Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán," Ciudad de Buenos Aires, Argentina. parasitologia@anlis.gov.ar
authors.forename: Silvana
authors.initials: S
authors.lastname: Carnevale
authors.forename: Jorge N
authors.initials: JN
authors.lastname: Velásquez
authors.forename: Hernando Del
authors.initials: HD
authors.lastname: Portillo
authors.forename: Jorge H
authors.initials: JH
authors.lastname: Labbé
authors.forename: Marta G
authors.initials: MG
authors.lastname: Cabrera
authors.forename: Marcela
authors.initials: M
authors.lastname: Ferella
authors.forename: Björn
authors.initials: B
authors.lastname: Andersson
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Guarnera
authors.forename: Sergio O
authors.initials: SO
authors.lastname: Angel
mesh_terms: D000330:Aedes / Q000469:parasitology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003465:Culex / Q000469:parasitology; D015342:DNA Probes / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015151:Immunoblotting; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D016780:Malaria, Vivax / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D015342:DNA Probes; D016054:DNA, Protozoan; D015800:Protozoan Proteins