title: Detection and molecular characterization of a gentamicin-susceptible, methicillin-resistant Staphylococcus aureus (MRSA) clone in Rio de Janeiro that resembles the New York/Japanese clone.
abstract: Staphylococcus aureus is the leading cause of hospital-acquired infections in many countries, and multiple factors contribute to the ability of these bacteria to disseminate and spread in hospitals. In Brazil it has been demonstrated that a multiresistant methicillin-resistant S. aureus clone, the so-called Brazilian epidemic clone, is widespread geographically. This clone was first detected in 1992 in Brazil, and recently from many other countries within South America, Europe and Asia. The study describes the detection of a gentamicin-susceptible heterogeneous MRSA clone that resembles another MRSA clone widely spread in US and Japanese hospitals, and supports the premise that the detection of heterogeneous MRSA isolates by some recommended methods is a challenging task that may, occasionally, result in MRSA misidentification.
authors.affiliation: Laboratório de Biologia Molecular de Bactérias, Instituto de Microbiologia Prof Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Cidade Universitária, Rio de Janeiro, RJ 21941-590, Brazil.
authors.forename: M C N
authors.initials: MC
authors.lastname: Melo
authors.forename: M C
authors.initials: MC
authors.lastname: Silva-Carvalho
authors.forename: R L
authors.initials: RL
authors.lastname: Ferreira
authors.forename: L R
authors.initials: LR
authors.lastname: Coelho
authors.forename: R R
authors.initials: RR
authors.lastname: Souza
authors.forename: C N
authors.initials: CN
authors.lastname: Gobbi
authors.forename: R
authors.initials: R
authors.lastname: Rozenbaum
authors.forename: C A
authors.initials: CA
authors.lastname: Solari
authors.forename: B T
authors.initials: BT
authors.lastname: Ferreira-Carvalho
authors.forename: A M S
authors.initials: AM
authors.lastname: Figueiredo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D016172:DNA Fingerprinting / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D018740:Genetic Heterogeneity; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D009518:New York / Q000453:epidemiology; D046915:Penicillin-Binding Proteins; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D005839:Gentamicins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus