Effect of all trans-retinoic acid on the secretion of IV collagenase.
BACKGROUND
Invasion and metastasis cause death of patients with liver cancer. Increased expression of matrix metalloproteinases (MMPs) is closely associated with tumor progression. Type IV collagen is the main structure protein of basilar membrane which is a natural barrier for inhibiting the metastasis of liver cancer cells. In this experiment, we used all trans-retinoic acid (ATRA) to inhibit collagenase type IV in order to protect the type IV collagen and basilar membrane, further to suppress the metastasis of hepatocellular carcinomas.


METHODS
By the use of cell culture and experimental animal models, the influence of all trans-retinoic acid (ATRA) on the invasion of Hca-F liver cancer cells was studied in terms of adhesion capacity to artificial basilar membrane and production of collagenase type IV.


RESULTS
ATRA could inhibit the adhesion capacity and collagenase IV secretion of Hca-F cells in a dose-dependent manner.


CONCLUSIONS
ATRA can exert multiple effects on the invasion of Hca-F cells.
Li
Bing
B
Department of General Surgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China. wilmawf@sina.com
Tian
Bo
B
D000818:Animals; D001489:Basilar Membrane; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D008113:Liver Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D008815:Mice, Inbred Strains; D009994:Osmolar Concentration; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*
D061965:Matrix Metalloproteinase Inhibitors; D018809:Proliferating Cell Nuclear Antigen; D014212:Tretinoin