Killing the messenger: antisense DNA and siRNA.
Among the technologies available for gene knockdown RNase H-dependent antisense oligonucleotides and RNAi are very popular. Both offer specificity and efficient knockdown of the genes; both are useful tools to study gene functions. Antisense and RNAi methods share many practical problems such as site selection, toxicity at high concentration, and the difficulty of transfection in certain cell types. We will focus in this review on the most important issues in the development of both methods and their possible use in gene-silencing therapy.
Nesterova
M
M
Cellular Biochem. Section, BRL, CCR, National Cancer Institute, Head, NIH Therapeutic Oligo. Int. Group, Bldg. 10, Rm. 5B05, 9000 Rockville Pike, Bethesda, MD 20892-1750, USA.
Cho-Chung
Y S
YS
D016373:DNA, Antisense / Q000627:therapeutic use*; D020868:Gene Silencing; D006801:Humans; D034741:RNA, Small Interfering / Q000627:therapeutic use*
D016373:DNA, Antisense; D034741:RNA, Small Interfering