title: Synapsin IIb interacts with the C-terminal SH2 and SH3 domains of PLCgamma1 and inhibits its enzymatic activity.
abstract: To elucidate the function of PLCgamma1, we have investigated the proteins that bind to its SH (Src homology) domain. Immunoscreening was performed with purified antisera specific for SH223 (two SH2 and one SH3)-binding proteins. Several immunoreactive clones were identified as putative binding proteins and one of them was identified as synapsin IIb. We demonstrate a stable association between PLCgamma1 and synapsin IIb, which binds the carboxyl terminal SH2 and SH3 domains of the enzyme and inhibits it.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Han
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Hong
authors.forename: Chul Geun
authors.initials: CG
authors.lastname: Kim
authors.forename: Jung Bin
authors.initials: JB
authors.lastname: Lee
authors.forename: Dong Kug
authors.initials: DK
authors.lastname: Choi
authors.forename: Kyong-Rae
authors.initials: KR
authors.lastname: Kim
authors.forename: Chan Gil
authors.initials: CG
authors.lastname: Kim
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000276:immunology / Q000502:physiology; D004791:Enzyme Inhibitors / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D007120:Immunochemistry; D051966:Phospholipase C gamma; D011485:Protein Binding / Q000276:immunology / Q000502:physiology; D011817:Rabbits; D051381:Rats; D016704:Synapsins / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D018909:src Homology Domains / Q000276:immunology*
chemical_list: D004791:Enzyme Inhibitors; D016704:Synapsins; D010738:Type C Phospholipases; D051966:Phospholipase C gamma