Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway.
3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.
Clingenpeel
Scott R
SR
Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA.
Keener
William K
WK
Keller
Caron R
CR
De Jesus
Karl
K
Howard
M Hope
MH
Watwood
Mary E
ME
D001565:Benzoates / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010105:Oxygenases / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
D001565:Benzoates; D005456:Fluorescent Dyes; D014050:Toluene; D010105:Oxygenases; C067624:xylene monooxygenase