Cold-inducible selective degradation of peroxisomes in Hansenula polymorpha.
Exposure of Hansenula polymorpha cells, grown in batch cultures on methanol at 37 degrees C, to a cold treatment (18 degrees C) is paralleled by a rapid degradation of peroxisomes present in these cells. Remarkably, the events accompanying organelle degradation at 18 degrees C are similar to those of selective glucose-induced peroxisome degradation in wild-type cells, described before. This observation was strengthened by the finding that cold-induced peroxisome degradation was not observed in mutants impaired in selective peroxisome degradation (Atg(-) mutants). Biochemical data indicated that the onset of peroxisome degradation was not triggered by the inactivation of peroxisome function due to the fall in temperature. We show that our findings have implications in case of fluorescence microscopy studies that are generally not conducted at physiological temperatures and thus may lead to strong morphological alterations unless proper precautions are taken.
Komduur
Janet A
JA
Laboratory of Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands.
Bellu
Anna Rita
AR
Knoops
Kevin
K
van der Klei
Ida J
IJ
Veenhuis
Marten
M
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D003080:Cold Temperature; D003576:Electron Transport Complex IV / Q000378:metabolism; D005560:Formate Dehydrogenases / Q000378:metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D020675:Peroxisomes / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D010843:Pichia / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure
D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C019643:alcohol oxidase; D005560:Formate Dehydrogenases; D003576:Electron Transport Complex IV