title: The association between HLA DQ genetic polymorphism and type 1 diabetes in a case-parent study conducted in an admixed population.
abstract: Susceptibility to the type 1 diabetes is genetically controlled and there is an increased risk associated with the presence of some specific alleles of the human leukocyte antigens class II loci (DQA1 and DQB1 genes). The purpose of this study is to evaluate the association between type 1 diabetes and HLA DQ alleles using case-parents trios in the admixed population of Uruguay composed by a mixture of Caucasian, Amerindian and Negroid populations. DQA1 and DQB1 genotyping was performed by polimerase chain reaction followed by oligospecific probes hybridization in 51 case-parents trios. The transmission disequilibrium test was used for detecting differential transmission in the HLA DQ loci. DQB1*0302 was the only allele for which preferential transmission is suggested (probability of transmission = 67.56%; exact p-value TDT = 0.047 uncorrected for multiple comparisons). DQA1*0301 allele showed a trend for preferential transmission without achieving statistical significance. This result would confirm the hypothesis previously advanced in a case-control study. Therefore, DQB1*0302 allele could be considered as the most important susceptibility allele for developing type 1 diabetes in Uruguay population.
authors.affiliation: Departamento de Citogenética, Unidad Asociada, Instituto de Biología, Facultad de Ciencias, Uruguay. abmg@iibce.edu.uy
authors.forename: Adriana
authors.initials: A
authors.lastname: Mimbacas
authors.forename: Fernando
authors.initials: F
authors.lastname: Pérez-Bravo
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Santos
authors.forename: Carmen
authors.initials: C
authors.lastname: Pisciottano
authors.forename: Rosario
authors.initials: R
authors.lastname: Grignola
authors.forename: Gerardo
authors.initials: G
authors.lastname: Javiel
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Jorge
authors.forename: Horacio
authors.initials: H
authors.lastname: Cardoso
mesh_terms: D000293:Adolescent; D044383:Black People / Q000235:genetics; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000453:epidemiology* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D007199:Indians, South American / Q000235:genetics; D008297:Male; D011110:Polymorphism, Genetic; D016036:Seroepidemiologic Studies; D014583:Uruguay / Q000453:epidemiology; D044465:White People / Q000235:genetics
chemical_list: D006683:HLA-DQ Antigens