title: Effect of the solvent on recognition properties of molecularly imprinted polymer specific for ochratoxin A.
abstract: A molecularly imprinted polymer specific for the mycotoxin ochratoxin A has been synthesised using a non-covalent approach. The polymer has shown an excellent affinity and specificity for the target template in aqueous solutions. The binding experiments, NMR study and molecular modelling have proven that the template recognition by polymer originates from the shape complementarity of binding sites. The binding mechanism is critically depended on factors that affect the polymer conformation. Thus the variation in buffer concentration, pH and presence of organic solvent, which affect the polymer swelling or shrinking, had a profound effect on the polymer recognition properties.
authors.affiliation: Institute of BioScience and Technology, Cranfield University, Silsoe, Bedfordshire MK45 4DT, UK. n.turner.1999@cranfield.ac.uk
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Turner
authors.forename: Elena V
authors.initials: EV
authors.lastname: Piletska
authors.forename: Khalku
authors.initials: K
authors.lastname: Karim
authors.forename: Michael
authors.initials: M
authors.lastname: Whitcombe
authors.forename: Michael
authors.initials: M
authors.lastname: Malecha
authors.forename: Naresh
authors.initials: N
authors.lastname: Magan
authors.forename: Claudio
authors.initials: C
authors.lastname: Baggiani
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Piletsky
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D003198:Computer Simulation; D008956:Models, Chemical; D008958:Models, Molecular; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D012997:Solvents / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009793:Ochratoxins; D011108:Polymers; D012997:Solvents; C025589:ochratoxin A