[The study of producing allogenic-tissue engineering cartilage].
OBJECTIVE
To explore the feasibility of producing neocartilage tissue by using allogenic chondrocytes as seeding cells, artificial absorbable scaffold-polylactic acid (PLA) as matrices.


METHODS
The cartilage tissue was obtained from the four limbs of neoborn New Zealand rabbits (born 3-5 days), digested by II collagenase, proliferated in vitro, then seeded the chondrocytes on PLA scaffolds. The chondrocyte-PLA complex were cultured 7-10 days in vitro, then implanted in dorsal subcutaneous of adult New Zealand rabbits. Specimens harvested 3 weeks, 5 weeks and 7 weeks after implantation respectively and subjected to histologic analysis.


RESULTS
Chondrocyte-PLA complex implanted in adult new Zealand rabbits for 7 weeks, produced neo-cartilage as proved by histologic analysis, along with time extending, chondrocyte matrice secretion increased, invading inflammatory cell decreased.


CONCLUSIONS
Using allogenic chondrocytes as seeding cells and PLA scaffold, it is successful to generate allogenic tissue-engineering cartilage in animals that have inherent immunity.
Lü
Wei
W
Department of Otorhinolaryngology, Peking Union Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
Zhang
Lianshan
L
Cai
Zhe
Z
Zhou
Tongliang
T
Li
Dan
D
D000818:Animals; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000166:cytology* / Q000637:transplantation; D020219:Chondrogenesis; D011100:Polyglycolic Acid; D011817:Rabbits; D023822:Tissue Engineering; D014184:Transplantation, Homologous
D011100:Polyglycolic Acid