title: Purification and characterization of alpha-keto amide reductase from Saccharomyces cerevisiae.
abstract: An NADPH-dependent alpha-keto amide reductase was purified from Saccharomyces cerevisiae. The molecular mass of the native enzyme was estimated to be 33 and 36 kDa by gel filtration chromatography and SDS-polyacrylamide gel electrophoresis, respectively. The purified enzyme showed a reducing activity not only for aromatic alpha-keto amides but also for aliphatic and aromatic alpha-keto esters. The internal sequence of the enzyme was identical with that of a hypothetical protein (ORF YDL 124w) coded by yeast chromosome IV.
authors.affiliation: Department of Life Science, Okayama University of Science, Okayama, Japan. ishihara@das.ous.ac.jp
authors.forename: Kohji
authors.initials: K
authors.lastname: Ishihara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Mitsuhashi
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Sadao
authors.initials: S
authors.lastname: Tsuboi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tsuji
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Nakajima
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification; D000645:Ammonium Sulfate; D004952:Esters / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007651:Keto Acids / Q000737:chemistry; D007700:Kinetics; D008968:Molecular Conformation; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011479:Protamines; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D004952:Esters; D007202:Indicators and Reagents; D007651:Keto Acids; D011479:Protamines; D029701:Saccharomyces cerevisiae Proteins; D000429:Alcohol Oxidoreductases; C500714:YDL124W protein, S cerevisiae; D000645:Ammonium Sulfate