title: Gemcitabine does not prevent acute rejection of the transplanted liver in rats.
abstract: Our study was designed to determine effect of gemcitabine on acute rejection of liver in rats. Liver transplantation was performed in rats of the Dark Agouti (DA) and Lewis (LEW) strains. Recipients were divided into three groups: A, DA-to-LEW without immunosuppression; B, DA-to-LEW, treated with cyclosporine A; C, DA-to-LEW, treated with gemcitabine. Immunosuppressants were subcutaneously injected for seven consecutive days after transplantation. On day 7, blood samples and liver graft tissue specimens were harvested. Group A showed severe rejection changes (RAI 8/9); in group B no rejection changes were present (RAI 0/9), and in group C moderate rejection changes were observed (RAI 6/9). Differences were significant between B vs C and A vs C groups; P<0.05. Serum creatinine and urea levels in the gemcitabine group were significantly lower than those in the cyclosporine A group. We did not confirm gemcitabine ability to prevent liver allograft rejection.
authors.affiliation: Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic. hynek.mergental@seznam.cz
authors.forename: Hynek
authors.initials: H
authors.lastname: Mergental
authors.forename: Jan
authors.initials: J
authors.lastname: Kriz
authors.forename: Eva
authors.initials: E
authors.lastname: Honsova
authors.forename: Michal
authors.initials: M
authors.lastname: Kudla
authors.forename: Tomas
authors.initials: T
authors.lastname: Pantoflicek
authors.forename: Katerina
authors.initials: K
authors.lastname: Tcherentsova
authors.forename: Matej
authors.initials: M
authors.lastname: Kocik
authors.forename: Frantisek
authors.initials: F
authors.lastname: Saudek
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ryska
mesh_terms: D000208:Acute Disease; D000818:Animals; D003404:Creatinine / Q000097:blood; D016572:Cyclosporine / Q000494:pharmacology; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006084:Graft Rejection / Q000473:pathology / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D017211:Treatment Failure; D014508:Urea / Q000097:blood; D000093542:Gemcitabine
chemical_list: D007166:Immunosuppressive Agents; D003841:Deoxycytidine; D016572:Cyclosporine; D014508:Urea; D003404:Creatinine; D000093542:Gemcitabine