title: Preparation of DNA chips using polyamine-oligonucleotide conjugates.
abstract: A convenient and efficient method for three-dimensional immobilizing oligonucleotides on glass was developed using oligonucleotide derivatives bearing a polyamine linker (PA-oligo conjugates). Polyamine (polylysine, poly(lysine, phenylalanine), polyethyleneimine) residues stipulate durable fixation of such conjugates to the glass surface with a high yield (90-95%). A DNA fragment (414-mer) is hybridized specifically to an immobilized oligonucleotide.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. Levina@niboch.nsc.ru
authors.forename: Asya
authors.initials: A
authors.lastname: Levina
authors.forename: Elena
authors.initials: E
authors.lastname: Mikhaleva
authors.forename: Valentina
authors.initials: V
authors.lastname: Zarytova
mesh_terms: D001483:Base Sequence; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D011073:Polyamines