title: Effects of hypervaccination with bovine herpesvirus type 1 gE-deleted marker vaccines on the serological response and virological status of calves challenged with wild-type virus.
abstract: Twenty-four calves were immunised four times with gE-deleted infectious bovine rhinotracheitis marker vaccines before being challenged with small doses of wild-type bovine herpesvirus type 1 (BHV-1). The repeated vaccinations induced strong immunity that prevented detectable virus replication and gE-seroconversion after the challenge infection in most of the calves. The hypervaccinated calves that shed virus after the challenge infection showed no delay in gE-seroconversion compared with unvaccinated control calves. Using a sensitive nested PCR, BHV-1 gE sequences could be detected in the trigeminal ganglia of several of the gE-seronegative, challenge-infected calves, possibly indicating the presence of wild-type BHV-1 DNA.
authors.affiliation: Department of Virology, Veterinary and Agrochemical Research Centre, Groeselenberg 90, B-1180 Brussels, Belgium.
authors.forename: J F
authors.initials: JF
authors.lastname: Toussaint
authors.forename: H J
authors.initials: HJ
authors.lastname: Rziha
authors.forename: B
authors.initials: B
authors.lastname: Bauer
authors.forename: C
authors.initials: C
authors.lastname: Letellier
authors.forename: P
authors.initials: P
authors.lastname: Kerkhofs
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011897:Random Allocation; D012668:Trigeminal Ganglion / Q000821:virology; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014779:Virus Replication; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins