title: Modulation of corneal epithelial cell functions by the neutrophil-derived inflammatory mediator CAP37.
abstract: OBJECTIVE
  To investigate the effect of CAP37, an inflammatory mediator in neutrophils, on three important events in corneal wound healing: proliferation, migration, and adhesion.
  
  
  METHODS
  Immortalized human corneal epithelial cells (HCEC) were treated with CAP37, and its effects on migration and proliferation were measured using the modified Boyden chemotaxis chamber and the proliferation assays (CyQUANT; Molecular Probes, Eugene, OR), respectively. Effects on adhesion were determined by measuring upregulation of adhesion molecules belonging to the selectin, integrin, and immunoglobulin superfamily using RT-PCR and flow cytometry.
  
  
  RESULTS
  CAP37 promoted proliferation of HCEC in a time- and dose-dependent fashion. CAP37 was maximally chemotactic for HCEC over a range of 1.3 x 10(-8) to 5.2 x 10(-8) M. CAP37 upregulated intercellular adhesion molecule (ICAM)-1, platelet endothelial cell adhesion molecule (PECAM)-1, and integrin molecules alpha3 (CD49c) and beta1 (CD29). Data on migration and ICAM-1 and PECAM-1 upregulation were corroborated using primary human corneal epithelial cells.
  
  
  CONCLUSIONS
  CAP37 modulated corneal epithelial cell proliferation and migration and upregulated adhesion molecules involved in leukocyte-epithelial and epithelial-extracellular matrix interactions.
authors.affiliation: Department of Pathology, BNSB 463, P.O. Box 26901, Oklahoma City, OK 73190, USA. anne-pereira@ouhsc.edu
authors.forename: H Anne
authors.initials: HA
authors.lastname: Pereira
authors.forename: Xin
authors.initials: X
authors.lastname: Ruan
authors.forename: Melva L
authors.initials: ML
authors.lastname: Gonzalez
authors.forename: Irina
authors.initials: I
authors.lastname: Tsyshevskaya-Hoover
authors.forename: James
authors.initials: J
authors.lastname: Chodosh
mesh_terms: D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000494:pharmacology*; D002352:Carrier Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D016023:Integrins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D018836:Inflammation Mediators; D016023:Integrins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1