[Relationship between GATA-3 and IL-4 and IL-5 in patients with allergic rhinitis].
OBJECTIVE
To evaluate the relationship between GATA-3 and IL-4, IL-5 in patients with allergic rhinitis.


METHODS
The expression of GATA-3 was detected by immunochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) in 23 patients with allergic rhinitis. IL-4, IL-5 were detected by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The positive rate of GATA-3 was 86.9% (20/23) and the relative density ratio of GATA-3 and glyceraldehyde phosphate dehydrogenase (GAPDH) were 0.602 +/- 0.11 in patients with allergic rhinitis. The concentrations of IL-4, IL-5 were (135.5 +/- 66.4) pg/mg, (77.5 +/- 29.4) pg/mg, respectively. The expressions of GATA-3 and IL-4, IL-5 had positive relationship (r = 0.45 and 0.62, P < 0.05).


CONCLUSIONS
GATA-3 contributed to the production of IL-4, IL-5 in patients with allergic rhinitis.
Li
Hua-bin
HB
Department of Otorhinolaryngology, Jiangsu Province Hospital, Nanjjing 210029, China. noseli@tom.com
Han
Den-min
DM
Zhou
Bing
B
Liu
Zhong-yan
ZY
D000328:Adult; D005260:Female; D050990:GATA3 Transcription Factor / Q000378:metabolism*; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology*; D015848:Interleukin-5 / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*
D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C508598:IL4 protein, human; C508599:IL5 protein, human; D015848:Interleukin-5; D015847:Interleukin-4