title: Regulation of procaspase-2 by glucocorticoid modulatory element-binding protein 1 through the interaction with caspase recruitment domain.
abstract: Caspases are the primary executioners of apoptosis. Although procaspases believe to exist as inactive forms in cells, the detailed regulatory system remains unclear. Here we show that glucocorticoid modulatory element-binding protein 1 (GMEB1) is capable of binding to the prodomain of caspase-2. We found that this molecule inhibits the autoproteolytic activation of procaspase-2 by oligomerization on a chemical compound-dependent system. These findings indicated that GMEB1 might be an endogenous inhibitory protein that selectively interacts with prodomain of caspase-2 to disrupt the autoactivation.
authors.affiliation: Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N12W6, Sapporo 060-0812, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tsuruma
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Hiromi
authors.initials: H
authors.lastname: Shirakura
authors.forename: Naoko
authors.initials: N
authors.lastname: Hayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Uehara
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nomura
mesh_terms: D017209:Apoptosis; D053143:Caspase 2; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C400298:p96 subunit, parvovirus initiation factor; D009703:Nucleoside-Phosphate Kinase; D053143:Caspase 2; D020169:Caspases