title: New directions of miniaturization within the biomarker research area.
abstract: An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel and liquid phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low-abundant protein expressions where assay miniaturization and integrated sample processing play a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.
authors.affiliation: Department of Analytical Chemistry, Lund Institute of Technology, Lund University, Sweden. gyorgy.marko-varga@astrazeneca.com
authors.forename: György A
authors.initials: GA
authors.lastname: Marko-Varga
authors.forename: Johan
authors.initials: J
authors.lastname: Nilsson
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D020869:Gene Expression Profiling; D006801:Humans; D056656:Lab-On-A-Chip Devices; D013058:Mass Spectrometry; D046208:Microchip Analytical Procedures / Q000379:methods*; D008904:Miniaturization; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*
chemical_list: D015415:Biomarkers; D011506:Proteins