The Bacillus subtilis DnaD protein: a putative link between DNA remodeling and initiation of DNA replication.
The Bacillus subtilis DnaD protein is an essential protein and a component of the oriC and PriA primosomal cascades, which are responsible for loading the main replicative ring helicase DnaC onto DNA. We present evidence that DnaD also has a global DNA architectural activity, assembling into large nucleoprotein complexes on a plasmid and counteracting plasmid compaction in a manner analogous to that recently seen for the histone-like Escherichia coli HU proteins. This DNA-remodeling role may be an essential function for initiation of DNA replication in the Gram +ve B. subtilis, thus highlighting DnaD as the link between bacterial nucleoid reorganization and initiation of DNA replication.
Turner
Ian J
IJ
Laboratory of Biophysics and Surface Analysis (LBSA), School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
Scott
David J
DJ
Allen
Stephanie
S
Roberts
Clive J
CJ
Soultanas
Panos
P
D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D014461:Ultracentrifugation
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C092895:DnaD protein, Bacillus subtilis; C448302:DnaI protein, Bacillus subtilis; D004265:DNA Helicases