title: Etest for assessing the susceptibility of filamentous fungi.
abstract: The purpose of this study was to evaluate the Etest as an in vitro antifungal susceptibility test method for different moulds originating from human samples and from the environment. A total of 50 isolates (1 Acremonium, 18 Aspergillus, 2 Cladosporium, 1 Epicoccum, 15 Penicillium, 2 Scopulariopsis and 11 Trichoderma strains) were tested by the Etest. Forty-six of the tested moulds (92%) were resistant to fluconazole with minimal inhibitory concentrations (MICs) > or = 256 microg ml(-1). There were strains resistant to ketoconazole among Aspergillus niger, A. ochraceus and Cladosporium spp. with MICs > 32 microg ml(-1). For fluconazole, no differences were observed using two different inocula, while for itraconazole, ketoconazole and amphotericin B, a 1 or less step 2-fold dilution difference in MIC was seen for the most of 10 selected strains. The MICs of fluconazole and amphotericin B obtained for Trichoderma strains by the Etest and the agar dilution method were also compared. MICs for fluconazole were in agreement, while MICs for amphotericin B were higher with 1 or 2 steps of 2-fold dilutions for most of Trichoderma strains in the case of the agar dilution method.
authors.affiliation: Department of Clinical Microbiology, Faculty of Medicine, University of Szeged, Somogyi Béla tér 1, H-6725 Szeged, Hungary.
authors.forename: Ilona
authors.initials: I
authors.lastname: Dóczi
authors.forename: Erika
authors.initials: E
authors.lastname: Dósa
authors.forename: J
authors.initials: J
authors.lastname: Varga
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Antal
authors.forename: L
authors.initials: L
authors.lastname: Kredics
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Nagy
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003470:Culture Media; D015725:Fluconazole / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects*; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D009181:Mycoses / Q000382:microbiology; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D017964:Itraconazole; D015725:Fluconazole; D007654:Ketoconazole