Modulation of corneal epithelial cell functions by the neutrophil-derived inflammatory mediator CAP37.
OBJECTIVE
To investigate the effect of CAP37, an inflammatory mediator in neutrophils, on three important events in corneal wound healing: proliferation, migration, and adhesion.


METHODS
Immortalized human corneal epithelial cells (HCEC) were treated with CAP37, and its effects on migration and proliferation were measured using the modified Boyden chemotaxis chamber and the proliferation assays (CyQUANT; Molecular Probes, Eugene, OR), respectively. Effects on adhesion were determined by measuring upregulation of adhesion molecules belonging to the selectin, integrin, and immunoglobulin superfamily using RT-PCR and flow cytometry.


RESULTS
CAP37 promoted proliferation of HCEC in a time- and dose-dependent fashion. CAP37 was maximally chemotactic for HCEC over a range of 1.3 x 10(-8) to 5.2 x 10(-8) M. CAP37 upregulated intercellular adhesion molecule (ICAM)-1, platelet endothelial cell adhesion molecule (PECAM)-1, and integrin molecules alpha3 (CD49c) and beta1 (CD29). Data on migration and ICAM-1 and PECAM-1 upregulation were corroborated using primary human corneal epithelial cells.


CONCLUSIONS
CAP37 modulated corneal epithelial cell proliferation and migration and upregulated adhesion molecules involved in leukocyte-epithelial and epithelial-extracellular matrix interactions.
Pereira
H Anne
HA
Department of Pathology, BNSB 463, P.O. Box 26901, Oklahoma City, OK 73190, USA. anne-pereira@ouhsc.edu
Ruan
Xin
X
Gonzalez
Melva L
ML
Tsyshevskaya-Hoover
Irina
I
Chodosh
James
J
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000494:pharmacology*; D002352:Carrier Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D016023:Integrins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015854:Up-Regulation
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D018836:Inflammation Mediators; D016023:Integrins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1