title: Chemiluminescent analysis of the antioxidant and immunomodulation effects of several psychotropic drugs on peritoneal macrophages.
abstract: The present study describes the application of several chemiluminescent (CL) methods for evaluation of antioxidant and immunomodulation effects of psychotropic drugs upon phagocytes: KO2-induced luminal-dependent CL for detection of superoxide anion radicals in a pure chemical system; PMA- and A23187-induced CL of peritoneal macrophages for detection of free radicals in cell suspension; and CL, produced by the luciferase-catalyzed luciferin + ATP reaction, for evaluation of cell viability before and after drug application. These methods provide also a way to investigate the location of drug action. It was found that the psychotropic drugs in fluence the 'oxidative burst' of macrophages through two mechanisms: by expression of drug antioxidant properties and/or by a direct immunomodulation effect.
authors.affiliation: Department of Medical Physics and Biophysics, Medical University, 2 Zdrave Str., Sofia 1431, Bulgaria. verah@mail.bg
authors.forename: V
authors.initials: V
authors.lastname: Hadjimitova
authors.forename: T
authors.initials: T
authors.lastname: Traykov
authors.forename: R
authors.initials: R
authors.lastname: Bakalova
authors.forename: V
authors.initials: V
authors.lastname: Petrova
authors.forename: I
authors.initials: I
authors.lastname: Lambev
authors.forename: H
authors.initials: H
authors.lastname: Ohba
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Baba
mesh_terms: D000639:Amitriptyline / Q000494:pharmacology; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D014150:Antipsychotic Agents / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology; D002749:Chlorprothixene / Q000494:pharmacology; D007099:Imipramine / Q000494:pharmacology; D007155:Immunologic Factors / Q000494:pharmacology*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007476:Ionophores / Q000494:pharmacology; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D011619:Psychotropic Drugs / Q000494:pharmacology*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013892:Thioxanthenes / Q000494:pharmacology
chemical_list: D000929:Antidepressive Agents, Tricyclic; D000975:Antioxidants; D014150:Antipsychotic Agents; D002147:Calmodulin; D007155:Immunologic Factors; D007202:Indicators and Reagents; D007476:Ionophores; D011619:Psychotropic Drugs; D013892:Thioxanthenes; D000639:Amitriptyline; D000001:Calcimycin; D008165:Luminol; D002749:Chlorprothixene; D013755:Tetradecanoylphorbol Acetate; D007099:Imipramine; D002746:Chlorpromazine