A maternal blood-borne factor promotes survival of the developing thalamus.
In this report, we describe the identification of a polypeptide survival-promoting factor that is produced by maternal and early postnatal peripheral blood mononuclear cells (PBMCs) of the immune system in Long-Evans rats and humans. The factor, termed Y-P30, most likely arises from proteolytic processing of a larger precursor protein and accumulates mainly in pyramidal neurons of the developing cortex and hippocampus but not in astrocytes. It was released from neurons grown in culture and substantially promotes survival of cells in explant monocultures of perinatal thalamus from the offspring. Y-P30 mRNA was not detectable in infant or adult brain and was present only in blood cells of pregnant rats and humans but not in nonpregnant controls. However, Y-P30 transcription could be induced in PBMCs of adult animals by a central nervous system lesion (i.e., optic nerve crush), which points to a potential role of the factor not only in neuronal development but also in neuroinflammation after white matter injury.
Landgraf
Peter
P
Institute of Physiology, Otto-von-Guericke University, Magdeburg, Germany.
Sieg
Frank
F
Wahle
Petra
P
Meyer
Gundela
G
Kreutz
Michael R
MR
Pape
Hans-Christian
HC
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000737:chemistry* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology; D006801:Humans; D007112:Immunity, Maternally-Acquired / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry / Q000378:metabolism; D009479:Neuropeptides / Q000096:biosynthesis / Q000097:blood / Q000276:immunology / Q000502:physiology; D009924:Organ Culture Techniques; D010455:Peptides / Q000097:blood* / Q000276:immunology / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D013534:Survival; D013787:Thalamic Nuclei / Q000737:chemistry / Q000166:cytology; D013788:Thalamus / Q000737:chemistry / Q000166:cytology / Q000196:embryology*; D014158:Transcription, Genetic / Q000502:physiology
D000906:Antibodies; D017077:Culture Media, Conditioned; D009479:Neuropeptides; D010455:Peptides; D011994:Recombinant Proteins; C442243:dermcidin