Functional analysis of the NPDC-1 gene.
Mouse NPDC-1 (Neural Proliferation Differentiation and Control-1) is specifically expressed in neural cells when they stop dividing and start to differentiate. The NPDC-1 protein has been shown to interact with the E2F1 transcription factor, D-type cyclins and Cdk2. Immunocytochemical studies and subcellular fractionation of rat brains disclosed a partial colocalization of NPDC-1 with synaptic vesicle proteins, suggesting additional functional interactions. Here, we report the characterization of the mouse and human genes that were found to display very similar structures. We mapped the human gene to chromosome 9q34.3. No obvious pathological defect has been previously linked to this region. In order to gain further insights into its function(s), we generated null mice for the NPDC-1 gene. We did not detect any macroscopic phenotypical defect. Analysis of the upstream sequence of the mouse NPDC-1 gene delineated two regions involved in its negative and positive transcriptional regulation. Evidence for the regulation of NPDC-1 by Krox family transcription factors is presented.
Evrard
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Laboratoire Biologie Moléculaire et Différenciation, Unité de Génétique Oncologique, CNRS-UMR 8125, Institut Gustave Roussy, PR-1, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France. clevrard@free.fr
Caron
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Rouget
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D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D002454:Cell Differentiation; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000502:physiology; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology; D013572:Synaptic Vesicles / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D009419:Nerve Tissue Proteins; C092313:Npdc1 protein, mouse; D014157:Transcription Factors