[Value of EBNA1-IgA and EA-IgG in serological diagnosis of nasopharyngeal carcinoma].
OBJECTIVE
To evaluate the value of EBNA1-IgA and EA-IgG in serological diagnosis of nasopharyngeal carcinoma (NPC).


METHODS
The serum EBNA1-IgA and EA-IgG of 56 patients with NPC and 58 healthy adults were detected by ELISA. The sensitivity, specificity, positive predictive value, accuracy rate and odds ratio of the two tests used singly or in combination were compared with each other.


RESULTS
The sensitivity of EBNA1-IgA (91.07%) was higher than that of EA-IgG (87.50%), while the specificity of EA-IgG (87.93%) was higher than that of EBNA1-IgA (84.48%). The combination of EBNA1-IgA and EA-IgG could enhance the specificity (94.83%), positive predictive value (0.9375), likelihood ratio (15.5435) and odds ratio (75.0000) for serological diagnosis of NPC. Forty-five patients showed both positive EBNA1-IgA and positive EA-IgG. A positive EA-IgG was detected in 4 out of 5 patients with negative EBNA1-IgA and a positive EBNA1-IgA was founded in 6 out of 7 patients with negative EA-IgG.


CONCLUSIONS
Although relatively high sensitivity and specificity could be obtained by either EBNA1-IgA or EA-IgG test alone, the combination of these two tests with a complementary effect is able to enhance the reliability of serological diagnosis of NPC as most patients have positive ENBA1-IgA and EA-IgG concurrently.
Zhang
Chang-Qing
CQ
Department of Experimental Research, Tumour Hospital, Sun Yat-sen University, Guangzhou 510060, China.
Zong
Yong-Sheng
YS
Sun
Yun
Y
Zhang
Yu
Y
Lin
Su-Xia
SX
Ye
Yong-Zhao
YZ
Feng
Kai-Tao
KT
Liang
Ying-Jie
YJ
D000328:Adult; D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; C033305:Epstein-Barr virus early antigen; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C417029:EBV-encoded nuclear antigen 1