Effects of castration, tooth resection, or tail docking on plasma metabolites and stress hormones in young pigs.
Changes in the activity of the sympathetic nervous system or in the hypothalamo-pituitary-adrenal axis have been extensively used to evaluate pain induced by castration or tail docking in numerous species. Such data are missing in pigs. Therefore, three experiments were conducted to determine the effects of castration, tail docking, or tooth resection on stress hormones. Glucose and lactate also were measured because catecholamines stimulate mobilization of glycogen, which results in glucose and lactate release. In Exp.1, 18 male pigs from seven litters (two or three pigs per litter) were catheterized surgically into one jugular vein, under general anesthesia, at 5 or 6 d of age. Two days later, they were submitted either to bilateral castration, control handling, or no handling (n = 6 per group). Blood samples were collected before (- 15 and -2 min) and after (5, 15, 30, 60, 90, 180 min) the experimental treatment. In Exp. 2, 27 female pigs from 12 litters (one to four pigs per litter) were submitted either to tooth clipping with pliers, tooth resection with a grinder apparatus, control handling, or no handling (n = 6 or 7 per group) at 1 d of age. In Exp. 3, 17 female pigs from nine litters (one to three pigs per litter) were submitted to one of the following treatments: 1) tail docking with an electric-heated scissor docking iron, 2) control handling, and 3) no handling (n = 5 or 6 per group) at 1 d of age. Castration induced significant (P < 0.05) increases in adrenocorticotropin hormone (ACTH; from 5 to 60 min), cortisol (from 15 to 90 min), and lactate (from 5 to 30 min). These variations are indicative of stress and tissue damage following castration. In contrast, neither tail docking nor tooth resection had marked effects on plasma cortisol, ACTH, glucose, and lactate. Measurements of plasma cortisol, lactate, and ACTH could be useful for validating treatments designed to relieve the distress reaction induced by castration in pigs.
Prunier
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UMR Veau et Porc, Institut National de la Recherche Agronomique, 35590 Saint-Gilles, France. armelle.prunier@rennes.inra.fr <armelle.prunier@rennes.inra.fr>
Mounier
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Hay
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D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002369:Castration / Q000662:veterinary*; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008297:Male; D013312:Stress, Physiological / Q000503:physiopathology / Q000662:veterinary*; D013552:Swine / Q000502:physiology*; D013623:Tail / Q000601:surgery*; D013997:Time Factors; D014070:Tooth / Q000601:surgery*
D001786:Blood Glucose; D019344:Lactic Acid; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone