title: Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices.
abstract: Synthetic polymer vascular patches used in cardiovascular surgery have shortcomings such as thrombosis, intimal hyperplasia, calcification, infection, and no growth potential. Tissue-engineered vascular patches using autologous vascular cells may solve these problems. In this study, we developed a tissue-engineered vascular patch using autologous bone marrow-derived cells (BMCs) and decellularized tissue matrices. Vascular smooth muscle cells and endothelial cells were differentiated from bone marrow mononuclear cells in vitro. Tissue-engineered vascular patches were fabricated by seeding these cells onto decellularized canine inferior vena cava matrices and implanted into the inferior vena cava of dogs. Three weeks after implantation, the tissue-engineered vascular patches were patent with no sign of thrombus formation. Histological, immunohistochemical, and electron microscopic analyses of the vascular patches retrieved 3 weeks after implantation revealed regeneration of endothelium and smooth muscle and the presence of collagen and elastin. BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular patches, indicating that the BMCs survived after implantation and contributed to the vascular tissue regeneration. This study demonstrates that vascular patches can be tissue-engineered with autologous BMCs and decellularized tissue matrices.
authors.affiliation: Department of Chemical Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Ryu
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: Cha Yong
authors.initials: CY
authors.lastname: Choi
authors.forename: Min-Jae
authors.initials: MJ
authors.lastname: Lee
authors.forename: Jong-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: In-Sung
authors.initials: IS
authors.lastname: Jang
authors.forename: Dong-Ik
authors.initials: DI
authors.lastname: Kim
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D002474:Cell-Free System / Q000637:transplantation / Q000648:ultrastructure; D002478:Cells, Cultured; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology; D005109:Extracellular Matrix / Q000637:transplantation* / Q000648:ultrastructure*; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D012038:Regeneration / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D014182:Transplantation, Autologous; D019737:Transplants; D014682:Vena Cava, Inferior / Q000254:growth & development* / Q000473:pathology / Q000601:surgery*