Roles of Toxoplasma gondii-derived heat shock protein 70 in host defense against T. gondii infection.
C57BL/6 mice receiving intraperitoneal injection of Toxoplasma gondii -derived heat shock protein 70 (T.g. HSP70) on day 3 post T. gondii infection succumbed by day 9 post infection, while vector protein-injected control mice survived more than 6 months. The deteriorating effect of T.g. HSP70 on host immune responses was dose-dependent. By T.g. HSP70 injection, T. gondii loads increased in various organs of T. gondii-infected mice. Th2 cytokines such as IL-4 and IL-10 were continuously produced from spleen and peritoneal exudate cells of T. gondii -infected mice by injection of T.g. HSP70. Furthermore, nitric oxide production from peritoneal macrophages in T. gondii-infected mice was reduced by T.g. HSP70.
Ahmed
Azza Kamal
AK
Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, Japan.
Mun
Hye-Seong
HS
Aosai
Fumie
F
Piao
Lian-Xun
LX
Fang
Hao
H
Norose
Kazumi
K
Yano
Akihiko
A
D000818:Animals; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D018840:HSP70 Heat-Shock Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D018418:Th2 Cells / Q000276:immunology; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D014123:Toxoplasmosis / Q000276:immunology* / Q000469:parasitology
D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D009569:Nitric Oxide