title: YAP4 gene expression is induced in response to several forms of stress in Saccharomyces cerevisiae.
abstract: Exposure of Saccharomyces cerevisiae to several environmental insults, including conditions of oxidative, heavy metal, metalloid and heat stress, induces the expression of the YAP4 gene, previously shown to play a role in the response to hyperosmotic stress. Expression analyses in several mutant strains under pro-oxidant conditions have determined that YAP4 is regulated by the transactivators Yap1p and Msn2p. Mutation of either the Yap1p-response element (YRE), located at - 517 bp from the ATG, or the most proximal stress response element (STRE) at -430 bp, is shown to strongly compromise YAP4 gene expression under these conditions. Furthermore, these two mutations in combination lead to a severe depletion of detectable mRNA levels, indicating interplay between the transcription factors Yap1p and Msn2p in the regulation of YAP4 transcription. Transcriptional activation of this gene reflects a concomitant increase in Yap4p protein levels that appear phosphorylated upon stress and negatively regulated by protein kinase A. Yap4p amino acid residues Ser89, Ser196 and Thr241 are shown to be required for protein phosphorylation and/or protein stability.
authors.affiliation: Stress and Genomics Laboratory, Instituto de Tecnologia Química e Biológica, Av. da República, Apt. 127, 2781-901 Oeiras, Portugal.
authors.forename: Tracy
authors.initials: T
authors.lastname: Nevitt
authors.forename: Jorge
authors.initials: J
authors.lastname: Pereira
authors.forename: Claudina
authors.initials: C
authors.lastname: Rodrigues-Pousada
mesh_terms: D004268:DNA-Binding Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D018384:Oxidative Stress; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C422266:Cin5 protein, S cerevisiae; D004268:DNA-Binding Proteins; C081935:MSN2 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae