Comparison of PCR- and HinfI restriction endonuclease-based methods for typing of Candida krusei isolates.
We compared HinfI restriction endonuclease-based analysis of genomic DNA with a PCR-based method for molecular typing of 90 Candida krusei isolates from 17 geographically related patients. Strain groupings by these methods were the same for 89 of 90 isolates. Ten of 17 patients were infected with related strains of C. krusei.
Sancak
Banu
B
Department of Clinical Microbiology and Microbiology, Hacettepe University Medical School, Ankara 06100, Turkey. banusancak@yahoo.com
Rex
John H
JH
Chen
Enuo
E
Marr
Kieren
K
D002175:Candida / Q000145:classification* / Q000235:genetics; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D003428:Cross Infection / Q000175:diagnosis / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006801:Humans; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping
D004271:DNA, Fungal; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases