[Stabilization of G x C-containing DNA duplexes by polyamides with parallel orientation in the minor groove].
The polyamides based on 4-amino-1-methylpyrrol-2-carboxylic acid, 4-amino-1-methylimidazole-2-carboxylic acid, and beta-alanine that stabilize oligonucleotide duplexes consisting of G x C pairs through parallel packing in the minor groove were studied. The initial duplex TTGCGCp x GCGCAA melts at 28 degrees C; the TTGCGCp[NH(CH2)3COPyIm betaImNH(CH2)3NH(CH3)2][NH(CH2)3COIm betaImPyNH(CH2)3N(CH3)2] x GCGCAA duplex (bisphosphoramidate with parallel orientation of ligands, where Py, Im, and beta are the residues of 1-methyl-4-aminopyrrol-2-carboxylic and 1-methyl-4-aminoimidazole-2-carboxylic acids and beta-alanine, respectively), at 48 degrees C; and the TTGCGCp[NH(CH2)3COIm betaImPyNH(CH2)3COIm betaImPyNH(CH2)3N(CH3)2] x GCGCAA duplex (a hairpin structure with antiparallel orientation), at 56 degrees C. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // www.maik.ru.
Siniakov
A N
AN
Butorin
A S
AS
Héléne
C
C
Riabinin
V A
VA
D000409:Alanine / Q000737:chemistry; D001482:Base Composition; D001665:Binding Sites; D001671:Biochemistry / Q000379:methods; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D004214:Distamycins / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D009757:Nylons / Q000737:chemistry*
D004214:Distamycins; D008024:Ligands; D009692:Nucleic Acid Heteroduplexes; D009757:Nylons; C030000:stallimycin; D004247:DNA; D000409:Alanine