title: [Effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro].
abstract: OBJECTIVE
  To study the effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro.
  
  
  METHODS
  MTT and PNPP were used separately to observe the proliferation and the activity of ALP of osteoblasts cultured in vitro with different concentration genistein for different incubation periods. Protein was measured by Lowry method.
  
  
  RESULTS
  It was found that genistein had the effects on promoting proliferation of osteoblasts cultured for 24 h and 48 h at concentration ranging from 1 x 10(-9) mol/L to 1 x 0(-5) mol/L. The activity of ALP was stimulated by genistein at concentration ranging from 1 x 10(-7) mol/L to 1 x 10(-5) mol/L after 72 h incubation.
  
  
  CONCLUSIONS
  Genistein has the effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro.
authors.affiliation: Institute of Combined Traditional Chinese and Western Medicine, Hebei Medical University, Shijiazhuang 050017.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Dejuan
authors.initials: D
authors.lastname: Kong
authors.forename: En
authors.initials: E
authors.lastname: Li
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Tong
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D007529:Isoflavones / Q000737:chemistry; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D019833:Genistein; D000469:Alkaline Phosphatase