title: Observations on the reproductive effects of once or twice weekly injections for 6 weeks of the GnRH agonist deslorelin in the cow.
abstract: This study investigated the reproductive effects in Holstein-Friesian cows of once or twice weekly intramuscular injection for 6 weeks of 100 microg of the GnRH agonist deslorelin at intervals. Oestrus was synchronized in non-lactating Holstein-Friesian cows before they were allocated randomly to receive either 100 microg deslorelin once weekly (D1; N=10) or twice weekly (D2; N=8) or acted as untreated controls (CON; N=8). The first injection was given on day 6 post-oestrus and the last injection on day 48 post-oestrus. Blood samples were collected twice weekly from each cow until day 76 after the synchronized oestrus to profile plasma P4. A single injection of prostaglandin was administered to all cows on day 20 post-oestrus to ensure luteolysis occurred. Ovaries were examined twice weekly by transrectal ultrasonography and then subsequently at weekly intervals to monitor ovarian structures. Progesterone profiles observed over two complete cycles for CON cows were typical of those expected for cows displaying regular oestrous cycles. Injection of deslorelin on day 6 post-oestrus induced ovulation in 100% (18/18; D1 and D2) of deslorelin-treated cows. Three categories of responses based on plasma P4 profiles were defined amongst the deslorelin-treated cows. Complete anoestrus was observed in 20% (2/10) of D1 and 63% (5/8) of D2 cows. A partial response characterised by intermittently low concentrations of P4 was observed in 50% (5/10) of D1 and 25% (2/8) of D2. A complete lack of response to deslorelin, with P4 profiles indistinguishable from CON cows, was seen in 30% (3/10) of D1 and 13% (1/8) of D2 cows. When results from D1 and D2 were pooled, a greater proportion of deslorelin-treated cows had abnormal ovarian cycles during the treatment period (56% versus 0%; D1 and D2 versus CON, P <0.001). In conclusion, the repeated injection of deslorelin either once or twice weekly for 6 weeks significantly altered the ovarian cycles of some cows; individual cow responses observed varied widely and ranged from complete anoestrus to a cycle indistinguishable from normal.
authors.affiliation: Veterinary Clinical Centre, University of Melbourne, Princes Hwy, Werribee 3030, Australia. andrew.padula@bristol.ac.uk
authors.forename: A M
authors.initials: AM
authors.lastname: Padula
authors.forename: K L
authors.initials: KL
authors.lastname: Macmillan
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D003008:Cloprostenol / Q000008:administration & dosage; D030762:Estrous Cycle / Q000187:drug effects; D004971:Estrus; D004973:Estrus Synchronization; D005260:Female; D007273:Injections, Intramuscular; D010062:Ovulation Induction / Q000662:veterinary; D011374:Progesterone / Q000097:blood; D012098:Reproduction / Q000187:drug effects*; D013997:Time Factors; D017329:Triptorelin Pamoate / Q000008:administration & dosage* / Q000031:analogs & derivatives*
chemical_list: D017329:Triptorelin Pamoate; D003008:Cloprostenol; D011374:Progesterone; C066438:deslorelin