title: Protein patterning based on electrochemical activation of bioinactive surfaces with hydroquinone-caged biotin.
abstract: We report a protein attachment and patterning method based on a hydroquinone-caged biotin surface that generates bioactive biotin by mild electrochemical perturbation. The electrochemical activation proceeds under the buffered aqueous environment at neutral pH. It also allows site-selective generation of bioactive biotin for the immobilization of target protein by using prepatterned electrode arrays.
authors.affiliation: Korea Research Institute of Standards and Science, Daejeon 305-600, Korea.
authors.forename: Kyuwon
authors.initials: K
authors.lastname: Kim
authors.forename: Haesik
authors.initials: H
authors.lastname: Yang
authors.forename: Sangyong
authors.initials: S
authors.lastname: Jon
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Kim
authors.forename: Juhyoun
authors.initials: J
authors.lastname: Kwak
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D004563:Electrochemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006873:Hydroquinones / Q000737:chemistry*; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D006873:Hydroquinones; D011506:Proteins; C058865:Strep-avidin conjugated horseradish peroxidase; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase; C031927:hydroquinone