Comparative analysis and development of microsatellite markers on swine (Sus scrofa) chromosome 1qter.
Several quantitative trait loci (QTL) have been detected on SSC1qter (Sus scrofa chromosome 1qter), including QTL for the number of vertebrae, as reported in our previous study. To provide the tools for analysis of QTLs on SSC1qter, we constructed a comparative map of swine and human. In addition, we identified 26 swine STSs and mapped 16 of them on SSC1qter using the INRA - University of Minnesota porcine radiation hybrid (IMpRH) panel. We screened a BAC library using these swine STSs and developed 35 new polymorphic microsatellite markers from the BAC clones, of which 26 were informative in our reference family. We also mapped nine microsatellite markers we had isolated previously. Consequently a total of 44 new polymorphic microsatellite markers were located within a 60-cM region of SSC1qter, spanning from SW1092 to the telomere.
Mikawa
S
S
Department of Genome Research, National Institute of Agrobiological Sciences, Ikenodai, Tsukuba, Ibaraki 305-0901, Japan. mikawa@affrc.go.jp
Shimanuki
S
S
Morozumi
T
T
Domukai
M
M
Shinkai
H
H
Uchida
Y
Y
Mikawa
A
A
Miyake
M
M
Miyake
Y
Y
Hayashi
N
N
Kusumoto
H
H
Uenishi
H
H
Hayashi
T
T
Awata
T
T
D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D033481:Chromosomes, Mammalian / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D040641:Quantitative Trait Loci; D034421:Sus scrofa / Q000235:genetics*