title: [Detection of genetically modified organisms obtained from food samples ].
abstract: Genetially modified organisms (GMOs) were explored in food samples obtained from November 2000 to March 2003 in the Tokyo area by using PCR and real-time PCR techniques. The existence of Roundup Ready Soybean (RRS) was surveyed in processed foods derived from soybeans, such as tofu, boiled soybean, kinako, nama-age, abura-age, natto, miso, soymilk and yuba. RRS was detected in 3 of 37 tofu, 2 of 3 nama-age, 2 of 3 yuba and 3 of 3 abura-age samples. The CBH351 in 70 processed corn foods, NewLeaf Plus and NewLeaf Y in 50 processed potato foods, and 55-1 papaya in 16 papayas were surveyed. These GMOs were not detected among the samples. Qualitative and quantitative analyses of RRS and genetically modified (GM) corn were performed in soybean, corn and semi-processed corn products such as corn meal, corn flour and corn grits. RRS was detected in 42 of 178 soybean samples, and the amount of RRS in RRS-positive samples was determined. The content was in the range of 0.1-1.4% in identity-preserved soybeans (non-GMO), and 49.8-78.8% in non-segregated soybeans. On the other hand, GM corns were detected in 8 of 26 samples. The amount of GM corn in GM corn-positive samples was in the range of 0.1-2.0%.
authors.affiliation: Tokyo Metropolitan Institute of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Kimio
authors.initials: K
authors.lastname: Monma
authors.forename: Rie
authors.initials: R
authors.lastname: Araki
authors.forename: Hisatsugu
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Sato
authors.forename: Naomichi
authors.initials: N
authors.lastname: Uno
authors.forename: Kazue
authors.initials: K
authors.lastname: Sato
authors.forename: Takashi
authors.initials: T
authors.lastname: Tobe
authors.forename: Hideo
authors.initials: H
authors.lastname: Kuribara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsuoka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hino
authors.forename: Kazuo
authors.initials: K
authors.lastname: Saito
mesh_terms: D029441:Carica / Q000235:genetics; D005504:Food Analysis / Q000379:methods*; D030841:Food, Genetically Modified; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods; D011198:Solanum tuberosum / Q000235:genetics; D045730:Soy Foods / Q000032:analysis*; D013025:Glycine max / Q000235:genetics*; D003313:Zea mays / Q000235:genetics