title: Differential expression of L- and T-type calcium channels between longitudinal and circular muscles of the rat myometrium during pregnancy.
abstract: The aim of this study was to evaluate the difference in the expression of mRNA of two types of calcium channels between longitudinal and circular muscle layer of rat myometrium during pregnancy. Changes in the expressions of the mRNA encoding L-type (alpha1C) and T-type (alpha1G, alpha1H, and alpha1I) calcium channels in longitudinal and circular smooth muscle cells of the rat myometrium were examined using a comparative kinetic RT/PCR method. During the course of pregnancy, alpha1C mRNA expression showed an N-shaped change in longitudinal muscle, but simply increased after mid-pregnancy in circular muscles. The mRNAs for alpha1G and alpha1H, but not that for alpha1I, were expressed in both longitudinal and circular smooth muscle. In longitudinal muscle, the change in alpha1H mRNA was similar to that in alpha1C mRNA during gestation, but the expression of alpha1G mRNA changed significantly only at term (day 22). In circular muscle, alpha1H mRNA expression was stable at any stage during pregnancy, but alpha1G mRNA significantly increased on day 15 and at term. No relationship was observed between voltage-dependent calcium-channel mRNA expressions and either proliferation or hypertrophy of circular muscle during pregnancy. These results show (a) that during pregnancy, the expression levels of L-type channels change dynamically, and it may contribute directly to the regulation of cell excitability, and (b) that the T-subtype that increases during pregnancy differs between longitudinal and circular muscle cells, although their functions remain unclear.
authors.affiliation: Department of Physiological Science & Molecular Biology, Fukuoka Dental College Fukuoka, Japan. ookut1@college.fdcnet.ac.jp
authors.forename: Tsuyako
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kawarabayashi
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Inoue
authors.forename: Kenji
authors.initials: K
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism*; D020747:Calcium Channels, T-Type / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D009215:Myometrium / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020746:Calcium Channels, L-Type; D020747:Calcium Channels, T-Type; D012333:RNA, Messenger