title: [Transient expression and characterization of intracellular single chain Fv against the nucleocapsid protein of Hantavirus].
abstract: OBJECTIVE
  To transiently express an intracellular single chain Fv of monoclonal antibody 1A8 against nucleocapsid protein of Hantavirus and characterize the immunological activities of the expressed products.
  
  
  METHODS
  COS-7 cells were transfected with mammalian expression vector 1A8-scFv-Ckappa/pCI-neo via lipofectin. The expressed product was identified by indirect immunofluorescence and immunoprecipitation.
  
  
  RESULTS
  A diffuse pattern fluorescence was observed in less than 1% cytoplasm of transfected COS-7 cells. The binding of intracellular antibody fragments to NP antigen was confirmed by immunoprecipitation analysis.
  
  
  CONCLUSIONS
  Transiently expressed single chain intrabodies can effectively target NP antigen in the cytoplasm. The present study may provide a new approach for treatment of Hantavirus.
authors.affiliation: Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Bai
authors.forename: Zhi-kai
authors.initials: ZK
authors.lastname: Xu
authors.forename: Fang-lin
authors.initials: FL
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Luo
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Xing-an
authors.initials: XA
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D019556:COS Cells; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism; D005822:Genetic Vectors; D006476:Orthohantavirus / Q000235:genetics / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D019590:Nucleocapsid Proteins