Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes.
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.
Thibault
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FM
Centre de Recherches du Service de Santé des Armées, 24 Avenue des maquis du Grésivaudan, B.P. 87, F-38702 La Tronche, France. fthibault@crssa.net
Valade
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Vidal
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DR
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D005896:Glanders / Q000382:microbiology; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D006801:Humans; D006868:Hydrolysis; D008554:Melioidosis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D001426:Bacterial Proteins; D015342:DNA Probes