Rapid identification of Borrelia burgdorferi sensu lato by PCR-restriction fragment length polymorphism analysis of groEL gene.
Thirty-one reference strains and 23 Korean isolates of the genus Borrelia were identified through the PCR-RFLP analysis using the groEL gene. This will be useful for the rapid differentiation of B. burgdorferi sensu lato and complements one of the 5S-23S intergenic spacers.
Park
Hyo-Soon
HS
Department of Microbiology, College of Medicine, Konkuk University, Seoul, Korea.
Lee
Jung-Hee
JH
Jeong
Eun-Ju
EJ
Park
Tae-Kyu
TK
Jang
Won-Jong
WJ
Park
Kyung-Hee
KH
Kim
Bum-Joon
BJ
Kook
Yoon-Hoh
YH
Lee
Seung-Hyun
SH
D000818:Animals; D015373:Bacterial Typing Techniques; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013997:Time Factors
D018834:Chaperonin 60; D004269:DNA, Bacterial