title: [Establishment of immortalized cell line BLTR-4 and primary identification of its biological character].
abstract: OBJECTIVE
  To establish immortalized cell line from the urothelium of the urinary bladder and identify the characteristics of the cell line.
  
  
  METHODS
  Human papillomavirus 16 (HPV-16) plasmid was used to transfect urothelium of infant urinary bladder in vitro with the help of Fugene-6, and this plasmid contained E6 and E7 genes of HPV-16. We also identified the existence of HPV-16 E6 and E7 genes and the biological characteristics of the cell line by PCR, immunohistochemistry, and the biology identification.
  
  
  RESULTS
  BLTR-4 cell line, produced from the transfection of HPV-16K plasmid, was a cell line from urothelium with the expression of HPV-16 E6 and E7 genes. It had been cultured more than 70 passages, and the characteristics of growth was similar to the immortalized cell line as reported.
  
  
  CONCLUSIONS
  BLTR-4 cell line is an immortalized cell line from urothelium of the urinary bladder, which contains HPV-16 E6 and E7 genes. BLTR-4 cell line is a good experimental model to investigate the relationship of the infection of high risk HPV and transitional cell carcinoma (TCC) in vitro.
authors.affiliation: Department of Pathology, Cancer Hospital, CAMS and PUMC, Beijing 100021, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zheng
authors.forename: Su-ping
authors.initials: SP
authors.lastname: Guo
authors.forename: Zu-gen
authors.initials: ZG
authors.lastname: He
authors.forename: Shu-jun
authors.initials: SJ
authors.lastname: Cheng
authors.forename: Yan-ning
authors.initials: YN
authors.lastname: Gao
mesh_terms: D002461:Cell Line, Transformed; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections / Q000821:virology; D010957:Plasmids / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014412:Tumor Virus Infections / Q000821:virology; D001743:Urinary Bladder / Q000166:cytology*; D001749:Urinary Bladder Neoplasms / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16