title: Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma.
abstract: The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.
authors.affiliation: Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA.
authors.forename: B
authors.initials: B
authors.lastname: Bindukumar
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kawinski
authors.forename: Craig
authors.initials: C
authors.lastname: Cherrin
authors.forename: Leah M
authors.initials: LM
authors.lastname: Gambino
authors.forename: Madhavan P N
authors.initials: MP
authors.lastname: Nair
authors.forename: Stanley A
authors.initials: SA
authors.lastname: Schwartz
authors.forename: Kailash C
authors.initials: KC
authors.lastname: Chadha
mesh_terms: D015153:Blotting, Western; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000737:chemistry / Q000302:isolation & purification*; D012661:Semen / Q000276:immunology*
chemical_list: D017430:Prostate-Specific Antigen