title: [Construction and expression of the eukaryotic coexpression plasmid containing Ag85B gene of Mycobacterium tuberculosis and IL-12 gene].
abstract: OBJECTIVE
  To construct an eukaryotic coexpression plasmid containing Mycobacterium tuberculosis (MTB) Ag85B and IL-12 genes.
  
  
  METHODS
  MTB Ag85B gene and IL-12 gene were cloned into pBudCE4.1 which has multiple promoters to construct recombinant plasmid pBud85B-IL12. The recombinant plasmid was transfected into COS-7 cells and the expression of target genes was assessed by RT-PCR and ELISA.
  
  
  RESULTS
  The expressions of Ag85B and IL-12 could be detected in COS-7 cells.
  
  
  CONCLUSIONS
  The recombinant plasmid pBud85B-IL12 was constructed and expressed successfully, which lays the foundation for further development of DNA vaccine against tuberculosis.
authors.affiliation: Department of Microbiology, Chongqing University of Medical Sciences, Chongqing 400016, China. meimei0075@sina.com.cn
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Dao-yin
authors.initials: DY
authors.lastname: Zhu
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Luo
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D018664:Interleukin-12 / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D018664:Interleukin-12; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis