title: Blending of a conventional Mycoplasma hyopneumoniae vaccine with a positive marker: tracking of immunised pigs by peptide-specific antibodies raised to the marker component.
abstract: Highly immunodominant marker antigens simply blended to existing veterinary vaccines may represent a smart approach for addressing the still open issue of vaccination compliance. This approach was evaluated by blending a widely deployed Mycoplasma hyopneumoniae vaccine with a peptide-KLH (Keyhole Limpet Hemocyanin) conjugate as marker. Piglets were vaccinated twice with: (i) a combination of the M. hyopneumoniae-specific vaccine and the marker, (ii) M. hyopneumoniae-specific vaccine, (iii) marker alone or (iv) placebo dose only. All piglets which received the M. hyopneumoniae-specific vaccine/marker formulation or, as control, the marker blended with Montanide IMS1313 adjuvant responded to the respective immunisation from day 21 to 77 post vaccination as seropositive for the appropriate peptide and KLH. However, the responder rate to M. hyopneumoniae of piglets administered with M. hyopneumoniae-specific vaccine/marker was slightly reduced at day 35 and 49 post immunisation in comparison with piglets vaccinated with M. hyopneumoniae-specific vaccine alone. Accordingly, we conclude that this marker technology could be successfully applied to label a whole set of vaccines prevented that the blending process will be optimised.
authors.affiliation: Responsif GmbH, Schallershofer Str. 84, D-91056 Erlangen, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Walders
authors.forename: Annett
authors.initials: A
authors.lastname: Raschke
authors.forename: Markus
authors.initials: M
authors.lastname: Neugebauer
authors.forename: Eugen
authors.initials: E
authors.lastname: Geuther
authors.forename: Wolf
authors.initials: W
authors.lastname: Bertling
authors.forename: Christian
authors.initials: C
authors.lastname: Reiser
authors.forename: Anja
authors.initials: A
authors.lastname: Buck
authors.forename: Sandra
authors.initials: S
authors.lastname: Strich
authors.forename: Juergen
authors.initials: J
authors.lastname: Hess
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D001428:Bacterial Vaccines / Q000276:immunology / Q000592:standards; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007114:Immunization / Q000662:veterinary*; D016056:Immunodominant Epitopes / Q000276:immunology; D008297:Male; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D015415:Biomarkers; D016056:Immunodominant Epitopes; D010455:Peptides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin