title: Effect of early wound excision on changes in plasma nitric oxide and endothelin-1 level after burn injury: an experimental study in rats.
abstract: The purpose of this study was to evaluate effects of early wound excision on changes in NO and endothelin-1 (ET-1) level in the plasma after extensive burn injury. The effects on vascular permeability and hepatic blood flow (HBF) were also assessed. Male Wistar rats were used for this study. A 30% total body surface area (TBSA) third-degree burn was made on the back. Then animals were divided into four groups. Burn group (n = 13), burn alone; infusion group (n = 13), burn injury and fluid resuscitation; early excision group (n = 13), burn injury, total wound excision at 30 min after the injury followed with immediate allogenic skin graft and fluid resuscitation; and the sham group (n = 15). The sham group and the early excision group did not show significant changes in the NO and ET-1 level in plasma during experimental period, while the burn group and the infusion group showed significant increase in the NO and ET-1. The early excision group also did not show hypovolemia, and the significant decrease in the HBF. These data suggest that the increased NO and ET-1 in plasma following thermal injury were originated from burned tissue and the removal of these injured tissue has beneficial effect on the vascular permeability and the changes in HBF.
authors.affiliation: Department of Burns, Beijing Jishuitan Hospital, Beijing 100035, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Soejima
authors.forename: Motohiro
authors.initials: M
authors.lastname: Nozaki
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tanabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakurai
mesh_terms: D000818:Animals; D002056:Burns / Q000097:blood / Q000503:physiopathology / Q000601:surgery*; D002199:Capillary Permeability / Q000502:physiology; D019332:Endothelin-1 / Q000097:blood*; D005440:Fluid Therapy / Q000379:methods; D008102:Liver Circulation / Q000502:physiology; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation / Q000379:methods; D014554:Urination / Q000502:physiology
chemical_list: D019332:Endothelin-1; D009569:Nitric Oxide