The structure and function of Xenopus NO38-core, a histone chaperone in the nucleolus.
Xenopus NO38 is an abundant nucleolar chaperone and a member of the nucleoplasmin (Np) family. Here, we report high-resolution crystal structures of the N-terminal domain of NO38, as a pentamer and a decamer. As expected, NO38 shares the Np family fold. In addition, NO38- and Np-core pentamers each use highly conserved residues and numerous waters to form their respective decamers. Further studies show that NO38 and Np each bind equal amounts of the four core histones. However, NO38 prefers the (H3-H4)(2) tetramer, while Np probably prefers H2A-H2B dimers. We also show that NO38 and Np will each bind noncognate histones when the preferred partner is absent. We suggest that these chaperones must form decamers in order to bind histones and differentiate between histone tetramers and dimers. When taken together, these data imply that NO38 may function as a histone chaperone in the nucleolus.
Namboodiri
V M Haridasan
VM
Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118, USA.
Akey
Ildikó V
IV
Schmidt-Zachmann
Marion S
MS
Head
James F
JF
Akey
Christopher W
CW
D000595:Amino Acid Sequence; D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D000090243:Nucleophosmin; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D014981:Xenopus
D006657:Histones; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D000090243:Nucleophosmin