Evaluation of the acidity constants of the 4-hidroxy-5-[salicylideneamino]-2-7-naphthalenedisulfonic acid (Azomethine-H) using UV-vis spectrophotometry.
The time stability of the azomethine-H species was determined not to be better than 10 min in the absence of oxygen and light, however under phosphate buffered conditions the azomethine-H species remained stable for longer periods, as indicated by the spectrophotometric behaviour. Nevertheless, the analysis time still exceeded the stability allowance. Therefore, the determination of the acidity constants of the Azomethine-H species was studied by means of UV-vis spectrophotometry in buffered media by means of the point-by-point analysis and data processing with SQUAD to refine the resulting constants, which were: pK(a1) = 3.39, pK(a2) 7.36 and pK(a3) 8.73. The latter were associated to the corresponding acid-base equilibria of the amine and hydroxy groups constituting the molecule.
Alarcón-Angeles
G
G
Departamento de Química, Universidad Autónoma Metropolitana (Iztapalapa), Av. San Rafael Atlixco #186, Col. Vicentina, C.P. 09340, Mexico D.F., Mexico.
Corona-Avendaño
S
S
Rojas-Hernández
A
A
Romero-Romo
M A
MA
Ramírez-Silva
M T
MT
D002021:Buffers; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009282:Naphthalenesulfonates / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D013053:Spectrophotometry; D013882:Thiosemicarbazones / Q000737:chemistry*; D017186:Titrimetry
D002021:Buffers; D009282:Naphthalenesulfonates; D010710:Phosphates; D013882:Thiosemicarbazones; C097966:azomethine H