[Prevalence of hemorrhagic fever with renal syndrome virus in domestic pigs: an epidemiological investigation in Shandong province].
OBJECTIVE
To investigate the epidemiological significance of hemorrhagic fever with renal syndrome virus (HFRSV) infection in domestic pigs in Shandong province, and study the role of domestic pigs in the prevalence of HFRS.


METHODS
Epidemiological investigation was performed in 4 cities of Shandong province. Reversed passive hemagglutination assay (RPHA), reversed passive hemagglutination inhibition (RPHI), HPR-SPA, immunofluorescent antibody (IFA), and reverse transcriptional PCR (RT-PCR) were used to detect antigen and antibody of HFRSV.


RESULTS
HFRSV antigen and antibody were detected in the heart, liver, lung, spleen, kidney, blood, urine, and stool of domestic pigs as well as in the sewage of the pigpen facilities. The positivity rate of HFRSV antigen ranged from 3.33% to 5.00% in the organ of pigs, and HFRSV positivity rate in the blood, urine, stool, and sewage was 3.67%, 7.04%, 2.51%, and 5.56%, respectively, with a total serum antibody positivity rate of 1.96%. The virus was isolated from the HFRSV antigen-positive samples, and could infect many organs after artificial infection of the pigs. HFRSV antigen can be detected in suckling rat brain and histopathological examination suggested transient pathological changes in such organs as the liver, lung and kidney. HFRSV may proliferate in pigs and was discharged through multiple routes.


CONCLUSIONS
Domestic pigs can be the host of HFRSV for the viral transmission.
Yang
Zhan-qing
ZQ
Department of Epidemiology, Southern Medical University, Guangzhou 510515, China.
Yu
Shou-yi
SY
Nie
Jun
J
Chen
Qing
Q
Li
Zhi-feng
ZF
Liu
Yun-xi
YX
Zhang
Jin-lan
JL
Xu
Jian-jiang
JJ
Yu
Xiao-ming
XM
Bu
Xiu-ping
XP
Su
Jing-jing
JJ
Zhang
Yun
Y
Tao
Kai-hua
KH
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000378:metabolism; D000956:Antigens, Viral / Q000378:metabolism; D002681:China / Q000453:epidemiology; D004197:Disease Reservoirs; D005260:Female; D018063:Hantaan virus / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology* / Q000635:transmission / Q000662:veterinary*; D008297:Male; D015995:Prevalence; D034421:Sus scrofa; D014018:Tissue Distribution
D000914:Antibodies, Viral; D000956:Antigens, Viral