New luminescent terbium complex for the determination of DNA.
New terbium complexes of derivatives of 2-oxo-4-hydroxy-quinoline-3-carboxylic acid are reported, which are highly luminescent, water soluble and do not require luminescence enhancers. The triplet-state energy levels of the ligands, the relative quantum yields (QYs) and the excitation maxima of the respective terbium chelates were determined. The large luminescence enhancement of one of these complexes by nucleic acids was investigated and a mechanism of its interaction with DNA is proposed. The optimal conditions for determination of DNA are equal concentrations of Tb(3+) and ligand R(1) (C = 1 x 10(-6) M), pH 9.0. Under optimal conditions the luminescence intensity (RI) is proportional to the concentration of fish sperm DNA (fsDNA) or calf thymus DNA (ctDNA), respectively, within the range of 0.05-1.5 microg ml(-1). The detection limits were 10 ng ml(-1) for fsDNA and 12 ng ml(-1) for ctDNA.
Yegorova
Alla
A
AV Bogatsky Physico-Chemical Institute of the National Academy of Sciences of Ukraine, 86, Lustdorfskaya doroga, Odessa 65080, Ukraine.
Karasyov
Alexander
A
Duerkop
Axel
A
Ukrainets
Igor
I
Antonovich
Valery
V
D000818:Animals; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004996:Ethidium / Q000032:analysis / Q000737:chemistry; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D049408:Luminescent Agents / Q000032:analysis / Q000737:chemistry*; D008297:Male; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013094:Spermatozoa; D013725:Terbium / Q000737:chemistry*
D008024:Ligands; D049408:Luminescent Agents; D013725:Terbium; D004247:DNA; D004996:Ethidium