1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method.
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was tested for interference with the Lowry method of protein determination. The EDC interference was nearly additive at both 660 and 750 nm. Blue-colored complex developed in the case of EDC and showed maximum absorbance at 750 nm, similar to the bovine serum albumin (BSA) estimation. In time response analysis, blue-colored complex was developed after 30 min of incubation in both cases (EDC and BSA); therefore, it followed the same kinetic pattern of color development as that of BSA. Interference is believed to be caused by the reduction of the folin-ciocalteu reagent by EDC, resulting in increased blue-colored complex and apparently causing increased protein content of the sample.
Kumar
Rakesh
R
Biomolecular Electronics and Nanotechnology Division (BEND), Central Scientific Instruments Organisation (CSIO), Sector 30-C, Chandigarh 160030, India. dabasrk@rediffmail.com
Shukla
Awdhesh Kumar
AK
Bagga
Ellis
E
Kumari
Sunita
S
Bajpai
Ram Prakash
RP
Bharadwaj
Lalit M
LM
D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry*; D005189:False Positive Reactions; D007700:Kinetics; D008982:Molybdenum / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine; D013997:Time Factors; D017973:Tungsten Compounds / Q000737:chemistry
C029556:Folin's phenol reagent; D011506:Proteins; D017973:Tungsten Compounds; D012710:Serum Albumin, Bovine; D008982:Molybdenum; D005022:Ethyldimethylaminopropyl Carbodiimide