title: Moraxella catarrhalis--infected alveolar epithelium induced monocyte recruitment and oxidative burst.
abstract: The recruitment of monocytes appears to be a crucial factor for inflammatory lung disease. Alveolar epithelial cells contribute to monocyte influx into the lung, but their impact on monocyte inflammatory capacity is not entirely clear. We thus analyzed the modulation of monocyte oxidative burst by A549 and isolated human alveolar epithelial cells. Epithelial infection with Moraxella catarrhalis induced monocyte adhesion, transepithelial migration, and superoxide generation, whereas stimulation with lipopolysaccharide, tumor necrosis factor-alpha, interleukin-1beta, or interferon-gamma induced adhesion or transmigration, but failed to initiate monocyte burst. The effect of microbial challenge was mimicked by phorbol myristate acetate and inhibited by the protein kinase C inhibitor bisindoylmaleimide. Furthermore, evidence for a role of platelet-activating factor-signaling in monocytes is presented. Monocyte burst was neither induced by supernatant nor affected by fixation of A549 cells, excluding the contribution of epithelium-derived soluble factors but emphasizing the mandatory role of intercellular contact. The employment of blocking antibodies, however, denied a role for the adhesion molecules intercellular adhesion molecule-1 and vascular cell adhesion molecule-1, or CD11b/CD18 and CD49d/CD29. In essence, infection of alveolar epithelial cells with M. catarrhalis might amplify the inflammatory capacity of invading monocytes eliciting their superoxide production. The epithelial response to this microbial challenge thus clearly differed from that to proinflammatory cytokines.
authors.affiliation: Department of Internal Medicine and Infectious Diseases, Charité-Campus Mitte, Schumannstrasse 20/21, 10117 Berlin, Germany. simone.rosseau@charite.de
authors.forename: Simone
authors.initials: S
authors.lastname: Rosseau
authors.forename: Kristina
authors.initials: K
authors.lastname: Wiechmann
authors.forename: Stefanie
authors.initials: S
authors.lastname: Moderer
authors.forename: Jochen
authors.initials: J
authors.lastname: Selhorst
authors.forename: Konstantin
authors.initials: K
authors.lastname: Mayer
authors.forename: Matthias
authors.initials: M
authors.lastname: Krüll
authors.forename: Andreas
authors.initials: A
authors.lastname: Hocke
authors.forename: Hortense
authors.initials: H
authors.lastname: Slevogt
authors.forename: Werner
authors.initials: W
authors.lastname: Seeger
authors.forename: Norbert
authors.initials: N
authors.lastname: Suttorp
authors.forename: Joachim
authors.initials: J
authors.lastname: Seybold
authors.forename: Jürgen
authors.initials: J
authors.lastname: Lohmeyer
mesh_terms: D002273:Carcinogens / Q000494:pharmacology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007249:Inflammation / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008171:Lung Diseases / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008262:Macrophage Activation; D008301:Maleimides / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D001936:Moraxella catarrhalis; D045828:Moraxellaceae Infections / Q000378:metabolism* / Q000473:pathology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016897:Respiratory Burst / Q000502:physiology; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D015815:Cell Adhesion Molecules; D016207:Cytokines; D007211:Indoles; D008070:Lipopolysaccharides; D008301:Maleimides; D013481:Superoxides; D011493:Protein Kinase C; C088060:bisindolylmaleimide; D013755:Tetradecanoylphorbol Acetate