Cyclase and phosphodiesterase activity on pre-T lymphoid human cells, treated with dimethyl sulfoxide (DMSO).
Our aim is to estimate the role of the DMSO on pre-T lymphoid human cells, we have searched the cyclase and phosphodiesterase activity. We have studied the GTPspecific cyclase (G-Case) and have observed an analogous course to that one of the cAMP-PDE, where, in both cases, the differences ratio is approximately 5. For the cyclase activity values it has been found that cAMP neo formed is undeterminable in these cells, for the controls and the treated samples.
Di Pietrantonio
F
F
Department of Applied Sciences of Oral and Dental Diseases, University G. D'Annunzio, Chieti, Italy.
Di Matteo
E
E
Di Nicola
M
M
Trubiani
O
O
Di Primio
R
R
Serra
E
E
Spoto
G
G
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D017209:Apoptosis; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D013997:Time Factors
D016166:Free Radical Scavengers; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine; D004121:Dimethyl Sulfoxide