title: Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway.
abstract: 3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.
authors.affiliation: Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Clingenpeel
authors.forename: William K
authors.initials: WK
authors.lastname: Keener
authors.forename: Caron R
authors.initials: CR
authors.lastname: Keller
authors.forename: Karl
authors.initials: K
authors.lastname: De Jesus
authors.forename: M Hope
authors.initials: MH
authors.lastname: Howard
authors.forename: Mary E
authors.initials: ME
authors.lastname: Watwood
mesh_terms: D001565:Benzoates / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010105:Oxygenases / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
chemical_list: D001565:Benzoates; D005456:Fluorescent Dyes; D014050:Toluene; D010105:Oxygenases; C067624:xylene monooxygenase