Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair.
DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place.
Mees
Alexandra
A
Department of Chemistry and Biochemistry, Butenandt-Strasse 5-13, Ludwig Maximilians University, D-81377 Munich, Germany.
Klar
Tobias
T
Gnau
Petra
P
Hennecke
Ulrich
U
Eker
Andre P M
AP
Carell
Thomas
T
Essen
Lars-Oliver
LO
D020029:Base Pairing; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry* / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D011740:Pyrimidine Dimers / Q000737:chemistry* / Q000378:metabolism; D046940:Synechococcus / Q000201:enzymology*; D013941:Thymine / Q000737:chemistry
D004277:DNA, Single-Stranded; D011740:Pyrimidine Dimers; D005182:Flavin-Adenine Dinucleotide; D004247:DNA; D004255:Deoxyribodipyrimidine Photo-Lyase; D013941:Thymine