title: Evaluation of A newly developed DOT-ELISA kit for the diagnosis of human brucellosis.
abstract: A new dot-ELISA kit for detection of Brucella antibodies in human sera was developed and compared with that of serum agglutination test, Rose Bengal plate test, rapid slide agglutination and Coomb's antiglobulin test. Following testing of 120 human sera from suspected patients of occupational risk, 25 gave positive reaction in Rose Bengal plate test, 25 in rapid slide agglutination test, 26 in serum agglutination test, 27 in Coomb's antiglobulin test and 28 in dot-ELISA kit. Dot-ELISA kit picked up more positive than any other Serological test, indicating its superiority over the other laboratory tests for the diagnosis of brucellosis.
authors.affiliation: Microbiology Dept, Defence Research and Development Establishment, Gwalior-474 002.
authors.forename: H V
authors.initials: HV
authors.lastname: Batra
authors.forename: G S
authors.initials: GS
authors.lastname: Agarwal
authors.forename: P V Ramchandran
authors.initials: PV
authors.lastname: Rao
mesh_terms: D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis*; D002002:Brucella / Q000276:immunology* / Q000302:isolation & purification; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis*; D003298:Coombs Test; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic
chemical_list: D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic