[Experimental study on apoptosis of keloid-derived fibroblast transducted with Fas gene by recombinant adenovirus].
OBJECTIVE
To generate recombinant adenovirus with human Fas gene and transfect the Fas gene into keloid-derived fibroblasts to replace the dysfunctional Fas gene for reconstruction of the blocked Fas signal.


METHODS
Fas gene was cut down from the PcDNA3.1-Fas plasmid. The recombinant adenovirus vector was constructed using PDC 315 system. Then the expression and function of the transfected Fas gene was detected.


RESULTS
We successfully constructed the recombinant adenovirus vector with Fas gene and detected its expression and function in the infected keloid-derived fibroblasts.


CONCLUSIONS
We have successfully generated recombinant adenovirus (Ad-PDC315-Fas), which can transfect the Fas gene into keloid-derived fibroblasis to replace the dysfunctional Fas gene and reconstruct the blocked Fas signal. The correlation between the keloid and Fas gene was confirmed. The result may set up a sound foundation for gene therapy of keloid.
Lu
Feng
F
Department of Plastic Surgery, Nanfang Hospital of First Military Medical University, Guangzhou 510515, China.
Gao
Jian-Hua
JH
D000256:Adenoviridae / Q000235:genetics; D017209:Apoptosis / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007627:Keloid / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics* / Q000378:metabolism; D019014:fas Receptor
D004274:DNA, Recombinant; C491901:FAS protein, human; D018124:Receptors, Tumor Necrosis Factor; D019014:fas Receptor