Monitoring of cAMP-imprinted polymer by fluorescence spectroscopy.
Conventional functional monomers together with fluorescent monomer, trans-4-[p-(N,N-dimethylamino)styryl]-N-vinylbenzylpyridinium chloride (vb-DMASP), were copolymerised in the presence of a target molecule, nucleotide-cAMP that acted as a molecular template. The polymer was copolymerised in thin-layer films. After removal of the template the functionalised cavities that exist in the fluorescent material are able to specifically bind the template. Subsequent adsorption of the template-cAMP causes quenching of fluorescence of the polymer. The specific photochemical processes accompanying the template adsorption are discussed further. The imprinted polymers monitored by both steady-state and time-resolved fluorescence techniques show specificity and selectivity of binding of the template on the imprinted functionalised cavities.
Wandelt
Barbara
B
Department of Molecular Physics, Faculty of Chemistry, Technical University of Lodz, Zeromskiego 116, Lodz 90 924, Poland. bwandelt@p.lodz.pl
Mielniczak
Alina
A
Cywinski
Piotr
P
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D000242:Cyclic AMP / Q000032:analysis* / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
C500828:4-(4-N,N-dimethylaminostyryl)-N-vinylbenzylopyridinium chloride; D000814:Aniline Compounds; D020099:Coated Materials, Biocompatible; D011726:Pyridinium Compounds; D000242:Cyclic AMP