Evaluation of the presence of a thapsigargin-sensitive calcium store in trypanosomatids using Trypanosoma evansi as a model.
Ca2+ plays an important role in the regulation of several important activities in different trypanosomatids. These parasites possess a Ca2+ transport system in the endoplasmic reticulum (ER) involved in Ca2+ homeostasis, which has been reported to be insensitive to thapsigargin, a classical inhibitor of the sarcoplasmic-ER Ca2+ adenosine triphosphatase (ATPase) (SERCA) in most eukaryotic cells. However, currently there is a controversy regarding the existence of a thapsigargin-sensitive ER Ca2+ store in these parasites. Therefore, we decided to explore the effect of this inhibitor using different methodological approaches. First, we selected Trypanosoma evansi as a parasite model to warrant the homogeneity of the population because this parasite has only a single life cycle, i.e., bloodstream-form trypomastigotes. Second, we compared the thapsigargin effect on Ca2+ homeostasis by spectrophotometrical Ca2+ measurements using 3 different approaches: whole-cell populations, cells that have been permeabilized by treatment with digitonin, and intact single cells. Our results demonstrate that a low concentration of thapsigargin induces Ca2+ release from intracellular Ca2+ stores in this parasite, which can be observed independently of the method used. Furthermore, the addition of thapsigargin before or after nigericin did not abolish its effect, showing that thapsigargin acts specifically on the ER. In conclusion, our results indicate the presence of a nonmitochondrial thapsigargin-sensitive Ca2+ store in T. evansi.
Mendoza
M
M
Universidad Nacional Experimental Simón Rodríguez, Instituto de Estudios Científicas y Tecnológicos, Centro de Estudios Biomédicas y Veterinarias, Caracas 1041A, Venezuela. memendoza@cantv.net
Mijares
A
A
Rojas
H
H
Colina
C
C
Cervino
V
V
DiPolo
R
R
Benaim
G
G
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects; D007476:Ionophores / Q000494:pharmacology; D009550:Nigericin / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology*; D014345:Trypanosoma / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D004791:Enzyme Inhibitors; D007476:Ionophores; D019284:Thapsigargin; D000252:Calcium-Transporting ATPases; D009550:Nigericin; D002118:Calcium