[Activation of nuclear factor-kappa B and expression of cytokine in intestinal epithelia stimulated by TNF-alpha].
OBJECTIVE
To investigate the effect of nuclear factor-kappa B activation on proinflammatory cytokine expression in intestinal epithelia when intestinal epithelia are in inflammatory condition.


METHODS
Colonic adenocarcinoma HT29 cells were cultured in wells and TNF-alpha (10 ng/ml) was added into half of the wells. The supernatants were collected and measured for IL-8 after 3 hours, nuclear factor-kappaB (NF-kappaB) P65 and IL-8 mRNA were also examined by Western blotting and RT-PCR respectively.


RESULTS
It was found that in the condition of no stimuli, there is little IL-8 mRNA expression of HT29 cells, P65 is seldom in HT29 cells' nuclei, and the concentration of IL-8 in supernatant is just 172 ng/L. However, after being stimulated by TNF-alpha, a lot of P65 expression in nuclei and a large number of IL-8 mRNA expression of HT29 cells are shown and the concentration of IL-8 in supernatant (639 ng/L) is significantly higher than that of control (P<0.01).


CONCLUSIONS
The activation of nuclear factor-kappa B modulates the expression of some proinflammatory cytokine in intestinal epithelia, and regulates epithelia function in inflammatory reaction.
Bai
Ai-ping
AP
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
Ouyang
Qin
Q
Gan
Hua-tian
HT
Wang
Chun-hui
CH
D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D019073:HT29 Cells / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha