title: UW is superior to Celsior and HTK in the protection of human liver endothelial cells against preservation injury.
abstract: Celsior solution (CS), a new preservation solution in thoracic organ transplantation, was evaluated for its efficacy in cold preservation of human liver endothelial cells (HLEC) and was compared to University of Wisconsin solution (UW) and histidine-tryptophan-ketoglutarate solution (HTK, Custodiol). HLEC cultures were preserved at 4 degrees C in CS, UW, and HTK, for 2, 6, 12, 24, and 48 hours, with 6 hours of reperfusion. Levels of lactate dehydrogenase (LDH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and adenosine 5'-triphosphate (ATP) were measured after each interval of ischemia and the respective phase of reperfusion. Preservation injury of HLEC as measured by LDH release, intracellular ATP level, and MTT reduction were overall significantly (P <or= .01, P <or= .01, P < .05, respectively) lower in UW than in CS and HTK. CS demonstrates a modest superiority to HTK in HLEC preservation. Furthermore, cold preservation remains the main cause of preservation injury of HLEC regardless of the preservation solution used. Additionally, the maintenance of a high intracellular ATP level of HLEC after ischemia and reperfusion, as shown by UW, could be taken as a beneficial effect, particularly in long-term ischemia. In conclusion, our cell culture model reveals the order of efficacy to protect HLEC against preservation injury as: UW >> CS > HTK.
authors.affiliation: Klinik für Allgemein- und Transplantationschirurgie, Universitätsklinikum Essen, Essen, Germany. hermann.janszen@t-online.de <hermann.janszen@t-online.de>
authors.forename: Hermann
authors.initials: H
authors.lastname: Janssen
authors.forename: Petra H E
authors.initials: PH
authors.lastname: Janssen
authors.forename: Christoph E
authors.initials: CE
authors.lastname: Broelsch
mesh_terms: D000241:Adenosine / Q000494:pharmacology* / Q000592:standards; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol / Q000494:pharmacology* / Q000592:standards; D002478:Cells, Cultured; D004396:Coloring Agents / Q000378:metabolism; D015925:Cryopreservation; D019610:Cytoprotection; D004187:Disaccharides / Q000494:pharmacology* / Q000592:standards; D004573:Electrolytes / Q000494:pharmacology* / Q000592:standards; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology* / Q000592:standards; D005971:Glutamates / Q000494:pharmacology* / Q000592:standards; D005978:Glutathione / Q000494:pharmacology* / Q000592:standards; D006639:Histidine / Q000494:pharmacology* / Q000592:standards; D006801:Humans; D007328:Insulin / Q000494:pharmacology* / Q000592:standards; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology* / Q000592:standards; D019188:Organ Preservation Solutions / Q000494:pharmacology* / Q000592:standards; D011189:Potassium Chloride / Q000494:pharmacology* / Q000592:standards; D011309:Preservation, Biological / Q000009:adverse effects; D011343:Procaine / Q000494:pharmacology* / Q000592:standards; D011887:Raffinose / Q000494:pharmacology* / Q000592:standards; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: C039984:Bretschneider cardioplegic solution; C088453:Celsior; D004396:Coloring Agents; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D007328:Insulin; D019188:Organ Preservation Solutions; D013778:Tetrazolium Salts; D013844:Thiazoles; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose