title: Dielectrophoretic micropatterning with microparticle monolayers covalently linked to glass surfaces.
abstract: Two-dimensional micropatterns of microparticles were fabricated on glass substrates with negative dielectrophoretic force, and the patterned microparticles were covalently bound on the substrate via cross-linking agents. The line and grid patterns of microparticles were prepared using the repulsive force of negative dielectrophoresis (n-DEP). The template interdigitated microband array (IDA) electrodes (width and gap 50 mum) were incorporated into the dielectrophoretic patterning cell with a fluidic channel. The microstructures on the glass substrates with amino or sulfhydryl groups were immobilized with the cross-linking agents disuccinimidyl suberate (DSS) and m-maleimidobenzoyl-N-hydroxy-succinimide ester (MBS). Diaphorase (Dp), a flavoenzyme, was selectively attached on the patterned microparticles using the maleimide groups of MBS. The enzyme activity on the patterned particles was electrochemically characterized with a scanning electrochemical microscope (SECM) in the presence of NADH and ferrocenylmethanol as a redox mediator. The SECM images proved that Dp was selectively immobilized onto the surface of microparticles to maintain its catalytic activity.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, 07 Aramaki-Aoba, Aoba-Ku, Sendai 980-8579, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Suzuki
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yasukawa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Mase
authors.forename: Daisuke
authors.initials: D
authors.lastname: Oyamatsu
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue