Effect of the solvent on recognition properties of molecularly imprinted polymer specific for ochratoxin A.
A molecularly imprinted polymer specific for the mycotoxin ochratoxin A has been synthesised using a non-covalent approach. The polymer has shown an excellent affinity and specificity for the target template in aqueous solutions. The binding experiments, NMR study and molecular modelling have proven that the template recognition by polymer originates from the shape complementarity of binding sites. The binding mechanism is critically depended on factors that affect the polymer conformation. Thus the variation in buffer concentration, pH and presence of organic solvent, which affect the polymer swelling or shrinking, had a profound effect on the polymer recognition properties.
Turner
Nicholas W
NW
Institute of BioScience and Technology, Cranfield University, Silsoe, Bedfordshire MK45 4DT, UK. n.turner.1999@cranfield.ac.uk
Piletska
Elena V
EV
Karim
Khalku
K
Whitcombe
Michael
M
Malecha
Michael
M
Magan
Naresh
N
Baggiani
Claudio
C
Piletsky
Sergey A
SA
D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D003198:Computer Simulation; D008956:Models, Chemical; D008958:Models, Molecular; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D012997:Solvents / Q000737:chemistry*; D013499:Surface Properties
D009793:Ochratoxins; D011108:Polymers; D012997:Solvents; C025589:ochratoxin A