Purification and characterization of a new reovirus from the Chinese mitten crab, Eriocheir sinensis.
A new reovirus was recently isolated from a freshwater crab, the Chinese mitten crab, Eriocheir sinensis, in China. The complete viral particles are 55 nm in diameter, icosahedral, non-enveloped and have a mean buoyant density of 1.39 g cm(-3) in CsCl gradient. The viral genome is composed of 12 pieces of dsRNA with an electrophoretic pattern of 3/4/2/3. This virus infects connective tissue of the gills, gut and hepatopancreas. Partial cDNA cloning and sequence analysis showed that the RNA-dependent RNA polymerase is located in the first RNA segment. From its biochemical, ultrastructural and physicochemical properties, this virus is quite different from the genus Aquareovirus (Reoviridae). It may represent a new genus of Reoviridae, different from the other crab reoviruses, P and W(2).
Zhang
S
S
Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
Shi
Z
Z
Zhang
J
J
Bonami
J-R
JR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000821:virology*; D002586:Cesium; D002681:China; D002712:Chlorides; D018076:DNA, Complementary / Q000235:genetics; D004064:Digestive System / Q000648:ultrastructure / Q000821:virology; D004586:Electrophoresis; D016679:Genome, Viral; D005880:Gills / Q000648:ultrastructure / Q000821:virology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012087:Reoviridae / Q000235:genetics* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA
D002712:Chlorides; D018076:DNA, Complementary; D002586:Cesium; D012324:RNA-Dependent RNA Polymerase; C028019:cesium chloride