Synergistic cytotoxicity of acidity and glucose degradation products in peritoneal dialysis fluid.
Of the nonphysiological compounds in glucose-rich peritoneal dialysate, we investigated the cytotoxicity and synergistic cytotoxicity of acidity and glucose degradation products (GDPs) using human peritoneal mesothelial cells (HPMC). The effect of pH on cell viability was examined by adding 1N HCl to a phosphate-buffered solution (pH > or = 5.5). We also examined the cytotoxic effects of various GDPs [glyoxal (GO), methylglyoxal (MGO), and 3-deoxyglucosone (3DG), alone or in combination] and pH (5.5 or 6.7). The cells were exposed to these solutions for 2 or 4 h. Cell viability was determined by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. Although the MTT viability of HPMC was not decreased by GDP or acidity alone, the combination of acidity and GDP markedly decreased MTT viability, strongly suggesting the synergistic cytotoxicity of GDP and acidity.
Okabe
Eiji
E
Second Department of Internal Medicine, Department of Infectious Diseases, Faculty of Medicine, Oita University, 1-1 Hasama, Oita 879-5593, Japan. okabey@med.oita-u.ac.jp
Tomo
Tadashi
T
Tezono
Kazuhito
K
Kikuchi
Hiroshi
H
Kadota
Jyun-ichi
J
Nasu
Masaru
M
D002470:Cell Survival; D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000737:chemistry; D004847:Epithelial Cells; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000166:cytology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D015314:Dialysis Solutions; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue