[Relationship between acute dipterex poisoning and oxidative stress and free radical damage].
OBJECTIVE
To study relationship between acute dipterex poisoning and oxidative stress and free radical damage.


METHODS
Eighty-two patients with acute dipterex poisoning (ADPP) and ninety-two healthy adult volunteers (HAV) were enrolled in the study with randomized controlled trial design. Plasma levels of vitamin C (VC) and vitamin E (VE), as well as level of lipoperoxide (LPO) and activities of superoxide dismutase (SOD) and acetylcholinesterase (AChE) in the red blood cells (RBC), were determined by spectrophotometry.


RESULTS
Levels of VC and VE, and activities of SOD and AChE were (37.35 +/- 9.98) micromol/L, (16.57 +/- 4.54) micromol/L, (1 785 +/- 154) U/g Hb and (213.1 +/- 57.6) U/g Hb, respectively, in the ADPP group, significantly lower than those in the HAV group, (55.34 +/- 15.98) micromol/L, (25.66 +/- 7.24) micromol/L, (2 124 +/- 185) U/g Hb and (305.3 +/- 83.6) U/g Hb, respectively. Plasma level of LPO was (35.20 +/- 5.29) nmol/g Hb in the ADPP group, significantly higher than that in the HAV group, (27.87 +/- 4.66) nmol/g Hb. Partial correlation analysis suggested that there existed negative correlation between activity of AChE in the RBC and plasma level of LPO (r = -0.274, P = 0.013) and positive correlation between activity of AChE in the RBC and plasma levels of VC and VE, and activity of SOD in the RBC (r = 0.333, P = 0.002, r = 0.269, P = 0.015 and r = 0.248, P = 0.026, respectively) in the ADPP, adjusted for age. Coefficient of reliability alpha was 0.682 (P < 0.001), with a standardized alpha of 0.868 (P < 0.001).


CONCLUSIONS
There exist severe oxidative stress and free radical damage in patients with acute dipterex poisoning.
Rui
Feng
F
Department of Internal Medicine, Pujiang County People's Hospital, Zhejiang 322200, China.
Zhou
Jun-fu
JF
Shao
Jian-min
JM
Zhang
Xue-ying
XY
He
Juan-fei
JF
D000110:Acetylcholinesterase / Q000097:blood; D000293:Adolescent; D000328:Adult; D001205:Ascorbic Acid / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005609:Free Radicals; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D011041:Poisoning / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood*; D014236:Trichlorfon / Q000506:poisoning*; D014810:Vitamin E / Q000097:blood
D005609:Free Radicals; D014810:Vitamin E; D014236:Trichlorfon; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D001205:Ascorbic Acid; D010100:Oxygen