Interactions of lipid monolayers with the natural biopolymer hyaluronic acid.
The interaction of the natural mucopolysaccharide hyaluronic acid with different lipids, present in the natural membranes, was studied at the lipid/water interface using thermodynamic methods and X-ray diffraction. The results show that this biopolymer modifies the properties and the structure of the lipid monolayer. The two-dimensional crystalline lattice and domain structure of the charged octadecylamine monolayer are strongly disturbed by the hyaluronic acid, the monolayer compressibility increases and the monolayer collapse pressure drops down. In addition, the presence of charged lipid interfaces influences the structural organisation of the hyaluronic acid at the membrane/water interfaces. The impacts of these results on the structural organisation at the membrane interface are discussed.
Ionov
R
R
LNPC, Université René Descartes, 45, rue des Saints Pères, 75270 Paris Cedex 06, France. r_ionov@hotmail.com
El-Abed
A
A
Goldmann
M
M
Peretti
P
P
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D002021:Buffers; D002417:Cattle; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D015394:Molecular Structure; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D000588:Amines; D001704:Biopolymers; D002021:Buffers; D008055:Lipids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D012997:Solvents; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; C038694:dipalmitoylphosphatidylserine; D006820:Hyaluronic Acid; C009317:stearylamine; C030345:1,2-dipalmitoylphosphatidylglycerol; C018525:1,2-dimyristoylphosphatidylethanolamine