Modulation of swarming and virulence by fatty acids through the RsbA protein in Proteus mirabilis.
After sensing external signals, Proteus mirabilis undergoes a multicellular behavior called swarming which is coordinately regulated with the expression of virulence factors. Here we report that exogenously added fatty acids could act as signals to regulate swarming in P. mirabilis. Specifically, while oleic acid enhanced swarming, some saturated fatty acids, such as lauric acid, myristic acid, palmitic acid, and stearic acid, inhibited swarming. We also found that expression of hemolysin, which has been shown to be coordinately regulated with swarming, was also inhibited by the above saturated fatty acids. Previously we identified a gene, rsbA, which may encode a histidine-containing phosphotransmitter of the bacterial two-component signaling system and act as a repressor of swarming and virulence factor expression in P. mirabilis. We found that while myristic acid, lauric acid, and palmitic acid exerted their inhibitory effect on swarming and hemolysin expression through an RsbA-dependent pathway, the inhibition by stearic acid was mediated through an RsbA-independent pathway. Biofilm formation and extracellular polysaccharide (EPS) production play an important role in P. mirabilis infection. We found that RsbA may act as a positive regulator of biofilm formation and EPS production. Myristic acid was found to slightly stimulate biofilm formation and EPS production, and this stimulation was mediated through an RsbA-dependent pathway. Together, these data suggest that fatty acids may act as environmental cues to regulate swarming and virulence in P. mirabilis and that RsbA may play an important role in this process.
Liaw
Shwu-Jen
SJ
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, 1st Section, Taipei, Taiwan, Republic of China.
Lai
Hsin-Chih
HC
Wang
Won-Bo
WB
D001426:Bacterial Proteins / Q000502:physiology*; D018441:Biofilms; D005227:Fatty Acids / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D011513:Proteus mirabilis / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D037521:Virulence Factors / Q000032:analysis
D001426:Bacterial Proteins; D005227:Fatty Acids; D011135:Polysaccharides, Bacterial; C116143:RsbA protein, Proteus mirabilis; D037521:Virulence Factors