[Mechanisms of sodium ferulate inhibition of collagen synthesis in hepatic stellate cells].
OBJECTIVE
To study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells.


METHODS
Collagen synthesis was analyzed by measuring 3H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor beta1 (TGFbeta1) level in cultured HSC-T6 cell. The effect of SFon the TGFbeta1 activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay.


RESULTS
SF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFbeta1. SF was shown to decrease TGFbeta1 level in the supernatant of HSC-T6 increased by oxidative stress. TGFbeta1 activity was intervened by SF.


CONCLUSIONS
SF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFbeta1 activity, and reduced the level of TGFbeta1 increased by oxidative stress.
Huang
Jin
J
Department of Pharmacy, Changhai Hospital, Shanghai 200433, China.
Hu
Jin-hong
JH
Qiu
Lei
L
Cai
Zhen
Z
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003373:Coumaric Acids / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008168:Lung / Q000166:cytology; D008907:Mink; D018384:Oxidative Stress; D051381:Rats; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
D003373:Coumaric Acids; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D003094:Collagen; C004999:ferulic acid