Comparison of mRNA expression levels determined with TaqMan and competitive template RT-PCR.
Two methods for measurement of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) mRNA expression were compared. Although the relative mRNA levels compared to beta-actin measured with competitive template RT-PCR were different from the data obtained with a TaqMan based PCR, a significant correlation between the two assays was found.
Mauritz
R
R
Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands.
Schwabe
W
W
Haeusler
P
P
Noordhuis
P
P
Smid
K
K
McLeod
H L
HL
Behnke
D
D
van Groeningen
C J
CJ
Peters
G J
GJ
D000199:Actins / Q000378:metabolism; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D015179:Colorectal Neoplasms / Q000378:metabolism; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013940:Thymidylate Synthase / Q000378:metabolism
D000199:Actins; D000964:Antimetabolites, Antineoplastic; D012333:RNA, Messenger; D042943:Dihydrouracil Dehydrogenase (NADP); D013940:Thymidylate Synthase; D005472:Fluorouracil