title: [Effect of ginsenoside Rg1 on the influence of neprilysin expression induced by LPS in BT325 cell line].
abstract: OBJECTIVE
  To investigate the effect of ginsenoside Rg1 on the influence of neprilysin expression induced by LPS in BT325 cell line.
  
  
  METHODS
  MTT was used to measure the levels of the survival rate of BT325 cultured with ginsenoside Rg1 and LPS in different concentrations. The expression of NEP was measured by RT-PCR.
  
  
  RESULTS
  The survival rate of BT325 was obviously inhibited by LPS, and the expression of NEP was decreased. The survival rate of BT325 was obviously raised by ginsenoside Rg1, and the expression of NEP was increased.
  
  
  CONCLUSIONS
  LPS can cause cell damage, and decrease the expression of NEP. Ginsenoside Rg1 can exert neuroprotective properties which protects BT325 cell line from the cell toxicity of LPS.
authors.affiliation: Neuropharmacological Research Laboratory, College of Pharmacy, Jinan University, Guangzhou 510632, China. tlhm@jnu.edu.cn
authors.forename: Huan-min
authors.initials: HM
authors.lastname: Luo
authors.forename: Hui
authors.initials: H
authors.lastname: Deng
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Li
authors.forename: Pei-fen
authors.initials: PF
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.forename: Wen
authors.initials: W
authors.lastname: Weng
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008070:Lipopolysaccharides; D015260:Neprilysin / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D015260:Neprilysin; C035054:ginsenoside Rg1