Urinary excretion of lignans after administration of isolated plant lignans to rats: the effect of single dose and ten-day exposures.
The difference in urinary excretion of mammalian and plant lignans in rats was determined after oral administration of equivalent doses (25 mg/kg of body weight) of 7-hydroxymatairesinol (HMR), lariciresinol (LAR), matairesinol (MR), and secoisolariciresinol (SECO). Twenty-four hours-urine samples were collected after a single dose and after administration of one dose/day for 10 days. Eight lignans were analysed in urine extracts using a high-performance liquid chromatography-tandem mass spectrometry method showing good sensitivity and repeatability. After a single dose of HMR, LAR, MR, and SECO, the main metabolites were 7-hydroxyenterolactone (HENL), cyclolariciresinol (CLAR), enterolactone (ENL), and enterodiol (END), respectively, but after 10-day exposure ENL was the main metabolite of all the tested lignans, showing a considerably higher excretion than after a single dose. Metabolic transformations of plant lignans into each other could also be observed.
Smeds
Annika I
AI
Department of Organic Chemistry, Abo Akademi University, Biskopsg. 8, FIN-20500 Turku/Abo, Finland. ansmeds@abo.fi
Saarinen
Niina M
NM
Hurmerinta
Teija T
TT
Penttinen
Pauliina E
PE
Sjöholm
Rainer E
RE
Mäkelä
Sari I
SI
D000818:Animals; D017705:Lignans / Q000008:administration & dosage / Q000652:urine*; D008297:Male; D010944:Plants / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D017705:Lignans