Binding of xenoestrogens to the sex steroid-binding protein in plasma from Arctic charr (Salvelinus alpinus L.).
A specific sex steroid-binding protein (SBP) is believed to be involved in regulation of circulating sex steroids, steroid delivery to target cells and intracellular signalling in sex steroid-sensitive tissues. In the present work, interactions between xenoestrogens and the plasma SBP in Arctic charr (Salvelinus alpinus L.) were determined using ligand-protein binding studies. The test compounds were all able to displace tritiated 17 beta-estradiol (E2) from the Arctic charr SBP (acSBP) in a competitive and dose-dependent manner. The acSBP affinities for the xenoestrogens ranged over several orders of magnitude (17 beta-estradiol>>ethynylestradiol (EE2)>zearalenone (ZEA)>diethylstilbestrol (DES)>genistein (GEN)>bisphenol A (BPA), 4-t-octylphenol (OP)>>o,p'-DDT, and dieldrin (DIN)), but were consistently lower than that of 17 beta-estradiol (about 4 x 10(2) -10(6)-fold less potent). The relative binding affinity (RBA) for selected chemicals were independent of both gender, age and maturation status, as well as variations of acSBP binding affinity. The affinity of endogenous steroids and estrogen mimics for the acSBP shows a high correlation to the affinity for the rainbow trout SBP, thus suggesting a phylogenetically conserved ligand-binding site between closely related species. Furthermore, it is argued that interaction with the acSBP- and SBP-mediated processes may introduce novel pathways for endocrine disruption, which may work in concert with the classical receptor-mediated effects.
Tollefsen
K-E
KE
Department of Biology, University of Oslo, P.O. Box. 1050 Blindern, N-0316 Oslo, Norway. knut.erik.tollefsen@niva.no
Ovrevik
J
J
Stenersen
J
J
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D045166:Estradiol Congeners / Q000378:metabolism; D004967:Estrogens / Q000097:blood / Q000378:metabolism*; D005260:Female; D008024:Ligands; D008297:Male; D011485:Protein Binding; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*; D014316:Tritium; D014337:Trout / Q000097:blood / Q000378:metabolism*; D015262:Xenobiotics / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology
D045166:Estradiol Congeners; D004967:Estrogens; D008024:Ligands; D012738:Sex Hormone-Binding Globulin; D015262:Xenobiotics; D014316:Tritium; D004958:Estradiol