Efficient cleavage of RNA, enhanced cellular uptake, and controlled intracellular localization of conjugate DNAzymes.
Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency (k(cat)/K(m)) and enhanced tolerance against DNase 1digestion. To be pointed out, intracellular localization of DNAzymes could be controlled by conjugated with naturally occurring signal peptides responsible for nuclear cytoplasmic transport of proteins.
Kubo
Takanori
T
Department of Biological and Environmental Chemistry, School of Science and Technology, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
Takamori
Kengo
K
Kanno
Ken-ichi
K
Bakalova
Rumiana
R
Rumiana
Bakalova
B
Ohba
Hideki
H
Matsukisono
Miho
M
Akebiyama
Yasuo
Y
Fujii
Masayuki
M
D001483:Base Sequence; D021881:DNA, Catalytic / Q000378:metabolism*; D015683:Gene Products, gag / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D006868:Hydrolysis; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*
D021881:DNA, Catalytic; D015683:Gene Products, gag; D012367:RNA, Viral