title: Micro patterning of cell and protein non-adhesive plasma polymerized coatings for biochip applications.
abstract: Micro scale patterning of bioactive surfaces is desirable for numerous biochip applications. Polyethyleneoxide-like (PEO-like) coating with non-fouling functionality has been deposited using low frequency AC plasma polymerization. The non-fouling properties of the coating were tested with human cells (HeLa) and fluorescence labeled proteins (isothiocyanate-labeled bovine serum albumin, i.e. FITC-BSA). The PEO-like coatings were fabricated by plasma polymerization of 12-crown-4 (ppCrown) with plasma polymerized hexene (ppHexene) as adhesion layer. The coatings were micro patterned using conventional cleanroom photolithography and lift-off. Single cell arrays showed sharp contrast in cell adhesion between the untreated glass surface and the ppCrown layer. Similarly, proteins adsorbed selectively to untreated glass but not to ppCrown. The simplicity of the lift-off technique and the sturdiness and versatility of the plasma-polymerized coatings, make this technology highly suitable for bio-MEMS and biochip applications, where patterned high contrast non-fouling surfaces are needed.
authors.affiliation: Scandinavian Micro Biodevices ApS, Gammelgårdsvej 87C, DK-3520 Farum, Denmark. sab@smb.dk
authors.forename: S
authors.initials: S
authors.lastname: Bouaidat
authors.forename: C
authors.initials: C
authors.lastname: Berendsen
authors.forename: P
authors.initials: P
authors.lastname: Thomsen
authors.forename: S Guldager
authors.initials: SG
authors.lastname: Petersen
authors.forename: A
authors.initials: A
authors.lastname: Wolff
authors.forename: J
authors.initials: J
authors.lastname: Jonsmann
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D047248:Crown Compounds / Q000737:chemistry*; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D047248:Crown Compounds; D012710:Serum Albumin, Bovine