Enzymatic syntheses and selective hydrolysis of O-beta-D-galactopyranosides using a marine mollusc beta-galactosidase.
The use of crude extract of the hepatopancreas of Aplysia fasciata, a large mollusc belonging to the order Anaspidea containing a beta-galactosidase activity, was reported for the synthesis of different galactosides. Good yields with polar acceptors and the uncommon beta-1-3 selectivity in the transgalactosylation reactions with most of the acceptors were observed. A beta-1-2 selectivity in the hydrolytic conditions was also observed and discussed.
Giordano
Assunta
A
Istituto di Chimica Biomolecolare C.N.R., Via Campi Flegrei 34, 80072 Pozzuoli, Naples, Italy.
Tramice
Annabella
A
Andreotti
Giuseppina
G
Mollo
Ernesto
E
Trincone
Antonio
A
D000818:Animals; D001048:Aplysia / Q000201:enzymology*; D005690:Galactose / Q000378:metabolism*; D006868:Hydrolysis; D001616:beta-Galactosidase / Q000378:metabolism*
D001616:beta-Galactosidase; D005690:Galactose