[Polymorphisms and species specificity of D2S2944 and D1S2134 loci in Chinese Han population in Chengdu].
OBJECTIVE
To obtain the data of polymorphism distribution of the two STR loci D2S2944, D1S2134 in Chinese Han population in Chengdu and evaluate their usefulness in the field of forensic science.


METHODS
PCR, polyacrylamide gel electrophoresis (PAGE) and silver staining were used to analyze 120 unrelated individuals of Chinese Han ethnic group in Chengdu. Fourteen different animals: monkey, pig, dog, bull, goat, chicken, duck, fish, cat, rabbit, Guinea pig, mouse, eel and frog were selected as controls in this study for evaluating the species specificity of the two STR loci.


RESULTS
Eight alleles and twenty-two genotypes were found in D2S2944. The observed heterozygosity (h), discrimination power (DP), polymorphism information content (PIC), chance of paternity exclusion power (EP) were 0.824, 0.925, 0.78, 0.644 respectively. Ten alleles and twenty-six genotypes were observed in D1S2134. The h, DP, PIC and EP were 0.769, 0.920, 0.79, 0.543 respectively. The genotype distributions of the two loci were analyzed by some related software and no deviation from the Hardy-Weinberg equilibrium was observed. Evaluated by way of using different animals as controls, the two STR loci of human beings were found to have good specificity.


CONCLUSIONS
These data indicate that D1S2134 and D2S2944 are of good polymorphism, high EP and DP, and can be applied as the candidate genetic marks to forensic parentage testing and identification. The methods to analyze them are simple, dependable and repeatable.
Zhou
Xue-ping
XP
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Ying-bi
YB
Zhang
Wei-juan
WJ
Jia
Zheng-jun
ZJ
Hou
Yi-ping
YP
Wu
Jin
J
D000483:Alleles; D000818:Animals; D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005554:Forensic Medicine; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D013045:Species Specificity; D020080:Tandem Repeat Sequences