title: Direct detection of boldenone sulfate and glucuronide conjugates in horse urine by ion trap liquid chromatography-mass spectrometry.
abstract: A study of the equine phase II metabolism of the anabolic agent boldenone is reported. Boldenone sulfate, boldenone glucuronide and their C17-epimers were synthesised as reference standards in our lab and a method was developed for their detection in a horse urine matrix. Solid phase extraction was used to purify the analytes, which were then detected by ion trap LC/MS. Negative and positive ionisation mode MS(2) were used for the detection of sulfate and glucuronide conjugates, respectively. Boldenone sulfate and 17-epiboldenone glucuronide were detected as the major and minor phase II metabolites, respectively, in horse urine samples collected following the administration of boldenone undecylenate by intramuscular injection.
authors.affiliation: School of Chemistry, F11, University of Sydney, Camperdown, NSW 2006, Australia.
authors.forename: Fan
authors.initials: F
authors.lastname: Pu
authors.forename: Andrew R
authors.initials: AR
authors.lastname: McKinney
authors.forename: Allen M
authors.initials: AM
authors.lastname: Stenhouse
authors.forename: Craig J
authors.initials: CJ
authors.lastname: Suann
authors.forename: Malcolm D
authors.initials: MD
authors.lastname: McLeod
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D020719:Glucuronides / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D013739:Testosterone / Q000031:analogs & derivatives* / Q000652:urine*
chemical_list: D020719:Glucuronides; D013739:Testosterone; C013915:boldenone