title: Stability of coagulation factors in thawed, solvent/detergent-treated plasma during storage at 4 degrees C for 6 days.
abstract: OBJECTIVE
  Transfusion of fresh-frozen plasma is still a pillar in emergency medicine for using to prevent dilutional coagulopathy or disseminated intravascular coagulation after severe blood loss, but thawing procedures can delay its availability. On the other hand, the wastage of plasma, once thawed and not transfused within a defined time-period, represents an inefficient handling of economic resources and is contradictory to blood donor intentions. In this study we investigated the stability of coagulation factor activities and plasma protein levels during 6 days of storage of thawed solvent/detergent (S/D)-treated plasma at +4 degrees C. Our results may form the basis for reconsideration of expiry times of thawed S/D-treated plasma.
  
  
  METHODS
  Five units of S/D-treated plasma (Octaplas) were thawed and warmed to 20 degrees C, then recooled and stored at +4 degrees C for 6 days. The activities of coagulation factors II, V, VII, VIII, IX, X, XI and XII, fibrinogen, antithrombin (AT), protein C, protein S and von Willebrand factor antigen (vWF:Ag) were measured on days 0, 1, 2, 3 and 6.
  
  
  RESULTS
  Except for protein S, the activities of all coagulation factors and inhibitors were at least 0.5 U/ml during storage at 4 degrees C for 6 days. The mean levels, during storage, of factors IX, X, XI and XII, vWF:Ag, fibrinogen and protein C were at least 94%, and of factors II, V and VIII, and AT at least 78%, of the levels immediately after thawing; the activity of factor VII decreased to 83% and of protein S to 43% of the baseline values.
  
  
  CONCLUSIONS
  Thawed S/D-treated plasma stored at +4 degrees C for up to 6 days still contains sufficient coagulation activities and plasma proteins to be regarded as suitable for transfusion in the established indications.
authors.affiliation: Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria. christoph.buchta@meduniwien.ac.at
authors.forename: C
authors.initials: C
authors.lastname: Buchta
authors.forename: M
authors.initials: M
authors.lastname: Felfernig
authors.forename: P
authors.initials: P
authors.lastname: Höcker
authors.forename: M
authors.initials: M
authors.lastname: Macher
authors.forename: G F
authors.initials: GF
authors.lastname: Körmöczi
authors.forename: P
authors.initials: P
authors.lastname: Quehenberger
authors.forename: H
authors.initials: H
authors.lastname: Heinzl
authors.forename: P
authors.initials: P
authors.lastname: Knöbl
mesh_terms: D001779:Blood Coagulation Factors / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D001798:Blood Proteins / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D003902:Detergents; D006801:Humans; D066298:In Vitro Techniques; D010949:Plasma / Q000378:metabolism*; D012997:Solvents; D013997:Time Factors; D065227:Transfusion Reaction
chemical_list: D001779:Blood Coagulation Factors; D001798:Blood Proteins; D003902:Detergents; D012997:Solvents