[Study on the acute toxicity experiment of mice and anti-tumor function in vitro of the qinglongyi].
OBJECTIVE
To study the anti-tumor effect and acute toxicity in vitro of the separation compositions from qinglongyi.


METHODS
The conventional acute toxicity experiments of mice, the MTT [3-(4,5-dimethylthiazol-2 yl)-2,5-diphenylterazolium bromide, MTT] and the SRB (sulforhodamin B) were used to make preliminary selection to qinglongyi and its separation compositions.


RESULTS
The half-deadly dose (LD50, the half-lethiferous dose) of the chloroform separation composition in qinglongyi was 575.38 mg x kg(-1) (i.g.), and of the acetic ether separation compositions in qinglongyi was 1303.59 mg x kg(-1) (i.g.). From the other parts of separation compositions in qinglongyi, the LD50 were more than 5 g x kg(-1). When acetic ether separation composition of qinglongyi was at 100 microg x mL(-1) the growth inhibitory rate (GIR) was < 50% to the leukaemia cell HL-60 of human. When chloroform separation composition and the acetic ether separation compositions of qinglongyi was at 100 mg x mL(-1), GIR was 52% to the leukaemia cell HL-60, the gastric carcinoma cell BGC-823 and the cervical carcinoma cell Hela of human.


CONCLUSIONS
The separation composition of the chloroform and the acetic ether from qinglongyi have obvious, anti-tumor effect.
Liu
Wei
W
Postdotoral Research Station of Institute of Materia Medica, Harbin University of Commerce, Harbin 150076, China.
Lin
Wen-han
WH
Ji
Yu-bin
YB
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D001522:Behavior, Animal / Q000187:drug effects; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D005638:Fruit / Q000737:chemistry; D018922:HL-60 Cells / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D031324:Juglans / Q000737:chemistry; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D013274:Stomach Neoplasms / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal