Measurement of total antioxidant capacity in gingival crevicular fluid and serum in dogs with periodontal disease.
OBJECTIVE
To determine whether gingival crevicular fluid (GCF) and serum total antioxidant capacities (TACs) correlate with the degree of severity of periodontal disease in dogs.


METHODS
41 Toy and Miniature Poodles.


METHODS
After assessment of the degree of severity of naturally occurring periodontitis, GCF samples from both maxillary fourth premolars and a blood sample were collected from each dog. The condition of the periodontium of the entire dentition and at each site of GCF collection was recorded. Clinical parameters assessed included plaque index, gingival index, and probing depth. Radiographic analysis of alveolar bone level was also performed. Total antioxidant capacity was measured in GCF and serum samples by use of a commercial kit.


RESULTS
Dogs with gingivitis and minimal periodontitis had significantly higher TAC in GCF than dogs with advanced periodontitis. Bivariate regression analysis revealed significant negative correlations between TAC in GCF and clinical parameters and age. The TAC in serum was significantly negatively correlated with the degree of gingival inflammation but was not significantly correlated with age.


CONCLUSIONS
TAC in GCF is related to the degree of severity of periodontal disease in dogs. This is likely the result of release of reactive oxygen species by activated phagocytes and fibroblasts in the inflamed periodontal tissues. The results of our study suggest that the local delivery of antioxidants may be a useful adjunctive treatment for periodontitis in dogs.
Pavlica
Zlatko
Z
Clinic for Small Animal Medicine and Surgery, Veterinary Faculty, University of Ljubljana, Slovenia.
Petelin
Milan
M
Nemec
Alenka
A
Erzen
Damjan
D
Skaleric
Uros
U
D000367:Age Factors; D016301:Alveolar Bone Loss / Q000662:veterinary; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003773:Dental Plaque / Q000473:pathology / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood* / Q000378:metabolism*; D004285:Dogs; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D010510:Periodontal Diseases / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D010519:Periodontium / Q000000981:diagnostic imaging / Q000473:pathology; D011859:Radiography; D012044:Regression Analysis
D000975:Antioxidants