Thermal stability of triple helical DNAs containing 2'-deoxyinosine and 2'-deoxyxanthosine.
In this paper, we describe the synthesis and thermal stabilities of the triplexes containing either 2'-deoxyinosine (1) or 2'-deoxyxanthosine (3) in their second strands. It was found that the triplexes with the 2'-deoxy-5-methylcytidine(dM)*1:dC and dM*1:dA base triplets are thermally stable, but those containing the dM*1:T and dM*1:dG base triplets are unstable under both neutral and slightly acidic conditions. On the other hand, it was found that the oligonucleotide containing 3 could form thermally stable triplexes with the oligonucleotides that involve four natural bases opposite the sites of 3. The rank of the thermal stabilities of the triplexes was as follows: the triplex containing the dM*3:dC base triplet > that containing the dM*3:dA base triplet > that containing the dM*3:T base triplet > that containing the dM*3:dG base triplet.
Ueno
Yoshihito
Y
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Laboratory of Molecular Biochemistry, Yanagido 501-1193, Gifu, Japan.
Shibata
Aya
A
Matsuda
Akira
A
Kitade
Yukio
Y
D001482:Base Composition; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D003853:Deoxyribonucleosides / Q000737:chemistry*; D006358:Hot Temperature; D007293:Inosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013329:Structure-Activity Relationship
D003853:Deoxyribonucleosides; D007293:Inosine Triphosphate; C065360:2'-deoxyinosine triphosphate; C077801:2'-deoxyxanthosine; D004247:DNA