Highly sensitive and selective determination of 9, 10-phenanthrenequinone in airborne particulates using high-performance liquid chromatography with pre-column derivatization and fluorescence detection.
9,10-Phenanthrenequinone (PQ), is one of the components of diesel exhaust particulates, has potent harmful effects on human health. For the determination of PQ in airborne particulates, a highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed with fluorescence detection following the pre-column fluorescent derivatization. PQ was derivatized with benzaldehyde as a derivatizing reagent in the presence of ammonium acetate to give fluorescent 2-phenyl-1H-phenanthro [9,10-d] imidazole. The maximum fluorescence intensity of the derivative was observed by the reaction with 0.2M benzaldehyde and 0.5 M ammonium acetate at 100 degrees C for 30 min. The HPLC separation of fluorescent derivative of PQ was performed within 20 min on an ODS column with a mixture of acetonitrile-water (55:45, v/v) as a mobile phase. Highly sensitive and selective determination of PQ was attained with the detection limit of 5 fmol (S/N = 3). By the proposed method, PQ in airborne particulates was successfully determined in the range 0.26-0.30 ng/m3 (n = 3).
Kishikawa
Naoya
N
Department of Analytical Chemistry, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852-8521, Japan.
Wada
Mitsuhiro
M
Ohba
Yoshihito
Y
Nakashima
Kenichiro
K
Kuroda
Naotaka
N
D000393:Air Pollutants / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D010616:Phenanthrenes / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000393:Air Pollutants; D005456:Fluorescent Dyes; D010616:Phenanthrenes; C005399:9,10-phenanthrenequinone