title: A strong positive dendritic effect in a peptide dendrimer-catalyzed ester hydrolysis reaction.
abstract: The contribution of the dendritic structure in catalysis of ester hydrolysis was investigated with a systematic peptide dendrimer series of increasing generation number (G1-G4) containing a catalytic consensus sequence His-Ser in all branches. A strong positive dendritic effect was observed with up to 100-fold increased histidine reactivity between G1 and G4. Kinetic studies and isothermal calorimetric titration experiments showed that the strong positive dendritic effect resulted from cooperativity between binding and catalysis.
authors.affiliation: Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland.
authors.forename: Estelle
authors.initials: E
authors.lastname: Delort
authors.forename: Tamis
authors.initials: T
authors.lastname: Darbre
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Reymond
mesh_terms: D002151:Calorimetry / Q000379:methods; D002384:Catalysis; D004952:Esters / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D007093:Imidazoles / Q000737:chemistry; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004952:Esters; D007093:Imidazoles; D010455:Peptides; D006639:Histidine; C023439:4-methylimidazole