Good and poor mobilizing patients differ in mobilized CD34+ cell adhesion molecule profiles.
BACKGROUND
Alterations in expression of adhesion molecules are important in the trafficking of hematopoietic progenitors and probably in the mobilization process. Relatively little and conflicting data are currently available on the differences in expression between good and poor mobilizing patients.


METHODS
In this study, the expression of eight adhesion molecules on the collected CD34+ cells from 36 patients undergoing mobilization was determined.


RESULTS
Good mobilizing patients, defined as those who collected their target in one apheresis procedure, had significantly fewer cells that expressed CD11a (LFA-1) and CD54 (ICAM-1) and borderline fewer that expressed CD11c, CD49d (VLA-4), and CD49d (VLA-5). No differences were detected in CD11b (Mac-1), CD15s (sLe(x)), or CD62L (L-selectin). Linear regression analysis identified number of prior chemotherapy courses and expression of CD11a (LFA-1) as independent predictive factors for mobilization efficiency. Good and poor mobilizing patients had approximately the same number of total CD34+ cells collected and little difference in times to engraftment.


CONCLUSIONS
CD11a (LFA-1) expression inversely correlates with mobilization efficiency. Elucidation of the mechanism(s) underlying these observations will require further study.
Ford
Clyde D
CD
Utah Blood and Marrow Transplant Program and the Department of Pathology, LDS Hospital and the University of Utah, Salt Lake City, Utah 84143, USA. ldcford@ihc.com
Greenwood
Jay
J
Anderson
Jeffrey
J
Handrahan
Diana
D
Petersen
Finn Bo
FB
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D039041:Integrin alpha4beta1 / Q000032:analysis; D039081:Integrin alpha5beta1 / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008297:Male; D008875:Middle Aged
D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D039041:Integrin alpha4beta1; D039081:Integrin alpha5beta1; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1