Use of coherent control methods through scattering biological tissue to achieve functional imaging.
We test whether coherent control methods based on ultrashort-pulse phase shaping can be applied when the laser light propagates through biological tissue. Our results demonstrate experimentally that the spectral-phase properties of shaped laser pulses optimized to achieve selective two-photon excitation survive as the laser pulses propagate through tissue. This observation is used to obtain functional images based on selective two-photon excitation of a pH-sensitive chromophore in a sample that is placed behind a slice of biological tissue. Our observation of coherent control through scattering tissue suggests possibilities in multiphoton-based imaging and photodynamic therapy.
Dela Cruz
Johanna M
JM
Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Pastirk
Igor
I
Comstock
Matthew
M
Lozovoy
Vadim V
VV
Dantus
Marcos
M
D000818:Animals; D001190:Arylsulfonates; D003952:Diagnostic Imaging; D004867:Equipment Design; D006801:Humans; D007834:Lasers; D008892:Milk; D018482:Muscle, Skeletal; D012542:Scattering, Radiation
D001190:Arylsulfonates; C005047:pyranine