title: Investigation of the use of positron emission tomography for neuroreceptor imaging in rabbit eyes.
abstract: To determine whether positron emission tomography (PET) can be used for imaging of neuroreceptors in eyes of rabbits. PET imaging of dopamine D(2) receptor, dopamine transporter, serotonin(1A) receptor and sigma(1) receptor in the eyes and brain was performed using corresponding positron-emitting ligands in baseline, pretreatment and displacement conditions. The 4 radioligands outlined the eyes and brain in the baseline. Pretreatment resulted in a slight reduction (26-28%) in the uptake in the anterior segments of eyes. The binding of each radioligand in the iris-ciliary body and retina was confirmed by ex vivo autoradiography. However, the PET signal in the eyes was unexpectedly higher than the autoradiography signal. The identification of radioligand-neuroreceptor binding by PET in the rabbit eyes is not specific enough.
authors.affiliation: Positron Medical Center, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Wang
authors.forename: Kiichi
authors.initials: K
authors.lastname: Ishiwata
authors.forename: Motohiro
authors.initials: M
authors.lastname: Kiyosawa
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kawamura
authors.forename: Keiichi
authors.initials: K
authors.lastname: Oda
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsuno
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
mesh_terms: D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging; D002924:Ciliary Body / Q000000981:diagnostic imaging; D050483:Dopamine Plasma Membrane Transport Proteins; D005123:Eye / Q000000981:diagnostic imaging*; D007498:Iris / Q000000981:diagnostic imaging; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D026901:Membrane Transport Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D049268:Positron-Emission Tomography; D011817:Rabbits; D011869:Radioligand Assay; D044282:Receptor, Serotonin, 5-HT1A / Q000032:analysis*; D017448:Receptors, Dopamine D2 / Q000032:analysis*; D017480:Receptors, sigma / Q000032:analysis*; D012160:Retina / Q000000981:diagnostic imaging
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D017448:Receptors, Dopamine D2; D017480:Receptors, sigma; D044282:Receptor, Serotonin, 5-HT1A