title: Prostaglandin E2 activates channel-mediated calcium entry in human erythrocytes: an indication for a blood clot formation supporting process.
abstract: Prostaglandin E(2) (PGE(2)) is released from platelets when they are activated. Using fluorescence imaging and the patch-clamp technique, we provide evidence that PGE(2) at physiological concentrations (10(-10) M) activates calcium rises mediated by calcium influx through a non-selective cation-channel in human red blood cells. The extent of calcium increase varied between cells with a total of 45% of the cells responding. It is well known that calcium increases elicited the calcium-activated potassium channel (Gardos channel) in the red cell membrane. Previously, it was shown that the Gardos channel activation results in potassium efflux and shrinkage of the cells. Therefore, we conclude that the PGE(2) responses of red blood cells described here reveal a direct and active participation of erythrocytes in blood clot formation.
authors.affiliation: Institute for Molecular Cell Biology, Faculty of Medicine, Building 61, Saarland University, 66421 Homburg/Saar, Germany. biophysics@web.de
authors.forename: Lars
authors.initials: L
authors.lastname: Kaestner
authors.forename: Wiebke
authors.initials: W
authors.lastname: Tabellion
authors.forename: Peter
authors.initials: P
authors.lastname: Lipp
authors.forename: Ingolf
authors.initials: I
authors.lastname: Bernhardt
mesh_terms: D000814:Aniline Compounds / Q000494:pharmacology; D001777:Blood Coagulation; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002412:Cations; D015232:Dinoprostone / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007477:Ions; D008564:Membrane Potentials; D009203:Myocardial Infarction / Q000378:metabolism; D018408:Patch-Clamp Techniques; D013050:Spectrometry, Fluorescence; D020521:Stroke; D013997:Time Factors; D014966:Xanthenes / Q000494:pharmacology
chemical_list: D000814:Aniline Compounds; D015220:Calcium Channels; D002412:Cations; C409648:Fluo 4; D005456:Fluorescent Dyes; D007477:Ions; D014966:Xanthenes; D015232:Dinoprostone; D002118:Calcium