Reagentless electrochemical biosensor based on the multi-wall carbon nanotubes and nanogold particles composite film.
A novel method was used to prepare the nano-composite by assembling nanogold (NG) particles on the multiwall carbon nanotubes (MWNTs) surface. The nano-composite could be immobilized on a glassy carbon (GC) electrode to get a novel modified electrode. The electrode can easily immobilize the horseradish peroxidase (HRP) molecules to construct a reagentless biosensor. The NG particles in the composite film have a good biological compatibility. And due to the existence of quinone groups on the MWNTs surface, the MWNTs can promote the electron transfer between enzymes and electrode surface. The biosensor shows a good stability and responds to H2O2 in the range from 2.0 microM to 3.5 mM with a detection limit of 1.0 microM.
Zhu
Jun-Jie
JJ
Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing, 210093, China.
Xu
Jin-Zhong
JZ
Hu
Zheng
Z
Chen
Hong-Yuan
HY
D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004583:Electrons; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
D037742:Nanotubes, Carbon; D006861:Hydrogen Peroxide