Oral and intraperitoneal immunization with rotavirus 2/6 virus-like particles stimulates a systemic and mucosal immune response in mice.
We examined the use of a virus-like particle (VLP) as an immunogen by analysing the IgA and IgG response generated in serum, intestinal (fecal), pulmonary and uterine samples. The particle comprised two rotavirus capsid proteins (simian VP2 and murine VP6) generated using recombinant baculovirus expression of the two capsid proteins, which self-assembled into particulate VLP2/6. Mice were immunized orally or intraperitoneally (i.p.) with 0 or 100 microg VLP2/6 with or without 5 microg cholera toxin adjuvant. The results showed a systemic and mucosal immune response to VLP2/6 when administered i.p. and, to a lesser extent, when delivered orally which was not dependent on adjuvant use and further proves the concept of VLP2/6 as an effective immunogen.
Shuttleworth
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Reproduction Group, AgResearch Wallaceville, Upper Hutt, New Zealand.
Eckery
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Awram
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D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology; D002772:Cholera Toxin; D005260:Female; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007274:Injections, Intraperitoneal; D008168:Lung / Q000276:immunology; D051379:Mice; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D022243:Rotavirus Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014599:Uterus / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus; D014614:Vaccines, Synthetic; D002772:Cholera Toxin