Microvascular oxygen delivery and consumption following treatment with verapamil.
The microvascular distribution of oxygen was studied in the arterioles and venules of the awake hamster window chamber preparation to determine the contribution of vascular smooth muscle relaxation to oxygen consumption of the microvascular wall during verapamil-induced vasodilatation. Verapamil HCl delivered in a 0.1 mg/kg bolus injection followed by a continuous infusion of 0.01 mg.kg(-1).min(-1) caused significant arteriolar dilatation, increased microvascular flow and functional capillary density, and decreased arteriolar vessel wall transmural Po(2) difference. Verapamil caused tissue Po(2) to increase from 25.5 +/- 4.1 mmHg under control condition to 32.0 +/- 3.7 mmHg during verapamil treatment. Total oxygen released by the microcirculation to the tissue remained the same as at baseline. Maintenance of the same level of oxygen release to the tissue, increased tissue Po(2), and decreased wall oxygen concentration gradient are compatible if vasodilatation significantly lowers vessel wall oxygen consumption, which in this model appears to constitute an important oxygen-consuming compartment. These findings show that treatment with verapamil, which increases oxygen supply through vasodilatation, may further improve tissue oxygenation by lowering oxygen consumption of the microcirculation.
Hangai-Hoger
Nanae
N
Dept. of Bioengineering, 0412, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412, USA. nhangai@bioeng.ucsd.edu
Tsai
Amy G
AG
Friesenecker
Barbara
B
Cabrales
Pedro
P
Intaglietta
Marcos
M
D000818:Animals; D006224:Cricetinae; D008647:Mesocricetus; D008833:Microcirculation / Q000187:drug effects / Q000502:physiology; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000502:physiology*; D012867:Skin / Q000098:blood supply* / Q000378:metabolism*; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology*
D014665:Vasodilator Agents; D014700:Verapamil; D010100:Oxygen