Metalloprotease-disintegrin ADAM8: expression analysis and targeted deletion in mice.
ADAM8 (a disintegrin and metalloprotease 8, also referred to as MS2/CD156a) is a membrane-anchored metalloprotease that was first identified in a macrophage cell line and has been implicated in neurodegenerative diseases. Here, we evaluated the expression of ADAM8 during mouse development and generated mice lacking ADAM8 (Adam8-/- mice). During early mouse development, ADAM8 is expressed by maternal cells in the decidua and by trophoblast derivatives of the embryo but not in the derivatives of the inner cell mass. At later stages, prominent expression of ADAM8 is seen in the embryo proper, in the gonadal ridge, thymus, developing cartilage and bone, brain and spinal cord, and in the mesenchyme in close proximity to the branch point between the jugular vein and developing lymphatic vessels. Examination of Adam8-/- mice, however, revealed no major defects in these or other structures during development or in adult tissues and no evident pathological phenotypes.
Kelly
Kristine
K
Cell Biology Program, Sloan-Kettering Institute, New York, New York, USA.
Hutchinson
Gillian
G
Nebenius-Oosthuizen
Daniela
D
Smith
Andrew J H
AJ
Bartsch
Jörg W
JW
Horiuchi
Keisuke
K
Rittger
Andrea
A
Manova
Katia
K
Docherty
Andy J P
AJ
Blobel
Carl P
CP
D051722:ADAM Proteins; D000483:Alleles; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics*; D015153:Blotting, Western; D001846:Bone Development; D001842:Bone and Bones; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007763:Lac Operon; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008648:Mesoderm / Q000378:metabolism; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008957:Models, Genetic; D009154:Mutation; D013997:Time Factors; D014018:Tissue Distribution; D001616:beta-Galactosidase / Q000378:metabolism
D015703:Antigens, CD; D008565:Membrane Proteins; D001616:beta-Galactosidase; D051722:ADAM Proteins; C489402:Adam8 protein, mouse; D008666:Metalloendopeptidases