Chronic stress and social housing differentially affect neurogenesis in male and female rats.
Stress plays an important role in the development of affective disorders. Women show a higher prevalence for these disorders than men. The course of a depression is thought to be positively influenced by social support. We have used a chronic stress model in which rats received foot-shocks daily for 3 weeks. Since rats are social animals we hypothesised that 'social support' might reduce the adverse effects of chronic stress. To test this hypothesis, male and female rats were housed individually or socially in unisex groups of four rats. The proliferation marker bromodeoxyuridine (BrdU) was injected 2 weeks before the sacrifice to investigate if stress and social housing influenced the survival of proliferating cells in the dentate gyrus (DG). To investigate changes in proliferation, another group of rats was sacrificed the day after the last BrdU injection. Stress significantly decreased BrdU labelling in individually housed males and not significantly in socially housed males. In individually housed females stress increased BrdU labelling, which was prevented by social housing. The increase found in females is most likely caused by differences in survival rate, since cell proliferation was not affected by stress or housing conditions. These results indicate that social support can affect neurogenesis in both female and male rats, however in a different way.
Westenbroek
Christel
C
Department of Psychiatry, Graduate School of Behavioral and Cognitive Neurosciences, University of Groningen, Hanzeplein 1, P.O. Box 30.001, 9700 RB Groningen, The Netherlands. c.westenbroek@med.rug.nl
Den Boer
Johan A
JA
Veenhuis
Maarten
M
Ter Horst
Gert J
GJ
D000818:Animals; D001522:Behavior, Animal; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002908:Chronic Disease; D018891:Dentate Gyrus / Q000166:cytology* / Q000378:metabolism; D004597:Electroshock / Q000009:adverse effects; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006799:Housing, Animal; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009474:Neurons / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D012934:Social Isolation; D012944:Social Support; D013312:Stress, Physiological / Q000378:metabolism / Q000503:physiopathology*
D005904:Glial Fibrillary Acidic Protein; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine