[Study on mimotopes of E.coli lipopolysaccharide 2630].
OBJECTIVE
To screen mimotopes of E.coli lipopolysaccharide(LPS) 2630 from c7c phage display peptide library.


METHODS
The LPS mimotopes were screened from c7c phage display peptide library by using affinity chromatograph-purified polyclonal antibody against E.coli LPS 2630(L2630), and the antigenicity of selected clones was identified by ELISA.


RESULTS
After 3 rounds of biopanning, 12 out of 20 phage clones were identified as positive clones which could bind to polyclonal anti-L2630 antibody, and 5 of these 12 clones could bind to polyclonal anti-S.typhi LPS 7261(L7261) antibody. The deduced amino acid sequence analysis showed that 8 of 12 clones had the conservative sequence: X-DGLL-XX or X-EDGLL-X.


CONCLUSIONS
The peptides displayed on these phage clones can mimic the epitopes of L2630, and 5 of these phage clones mimic the common epitopes of L2630 and L7261.
Luo
Hai-bo
HB
Department of Immunology, First Military Medical University, Guangzhou 510515, China. seaman@fimmu.com
Zheng
Shan-gen
SG
Zhu
Ping
P
Fu
Ning
N
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D000939:Epitopes / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D018716:Molecular Mimicry; D019151:Peptide Library; D012485:Salmonella typhi / Q000737:chemistry / Q000276:immunology; D017422:Sequence Analysis, DNA
D000907:Antibodies, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D019151:Peptide Library