[Detection of the Salmonella invasion gene invA using molecular beacon probe].
OBJECTIVE
To detect Salmonella invasion gene invA.


METHODS
The invA gene of Salmonella was amplified with PCR reaction system containing the molecular beacon probe labeled with 6-carboxy fluorescein (6-FAM) at its 5' end and with (4-dimethylaminophenylazo)benzoic acid (DABCYL) at its 3' end, and the fluorescence signal was read at the end of PCR.


RESULTS
The fluorescence values of S.enteritidis, S. paratyphi A, S. paratyphi B, S.typhi antigen H, S.typhi, and enteroinvasive E.coli were 161.6, 104.5, 85.9, 83.1, 94.8 and 46.1 respectively. The results of PCR of Salmonella was consistent with that of agarose gel electrophoresis.


CONCLUSIONS
PCR with fluorogenic molecular beacon probe is rapid, specific, sensitive, and convenient for detecting Salmonella with invA gene.
Wan
Cheng-song
CS
Department of Microbiology, Southern Medical University, Guangzhou 510515, China. gzwcs@fimmu.com
Li
Jun-ai
JA
Luo
Jun
J
D001426:Bacterial Proteins / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification; D012480:Salmonella Infections / Q000382:microbiology
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; C075518:invA protein, Bacteria