Polo-like kinase-1: activity measurement and RNAi-mediated knockdown.
Polo-like kinase-1 (Plk-1) is an important cell cycle regulatory kinase that has been implicated in a multitude of cell cycle events. In this chapter we review those multiple functions of Plk-1 and describe the methods routinely used in our laboratory to purify Plk-1 from cellular lysates and measure Plk-1 kinase activity in vitro. In addition, we describe a method to analyze cell cycle progression after depletion of Plk-1 by RNA-interference in tissue culture cells.
van Vugt
Marcel A T M
MA
Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam, The Netherlands.
Medema
René H
RH
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D002460:Cell Line; D005434:Flow Cytometry; D005822:Genetic Vectors; D006801:Humans; D047468:Immunoprecipitation; D010766:Phosphorylation; D011494:Protein Kinases / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000097869:Polo-Like Kinase 1