Silencing of MDR 1 gene in cancer cells by siRNA.
Inhibition of p-glycoprotein (PGP) expression and reverse of multidrug resistance (MDR) phenotype in KB-8-5 cells by synthetic 21-bp double-stranded oligoribonucleotides were investigated. siRNA constructs for the efficient down regulation of MDR1 that are active in nanomolar concentrations and cause reversal of MDR phenotype in cells were developed.
Logashenko
E B
EB
Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. evg_log@niboch.nsc.ru
Vladimirova
A V
AV
Repkova
M N
MN
Venyaminova
A G
AG
Chernolovskaya
E L
EL
Vlassov
V V
VV
D001483:Base Sequence; D045744:Cell Line, Tumor; D020868:Gene Silencing / Q000502:physiology*; D019450:Genes, MDR; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000502:physiology*; D020112:Rhodamine 123 / Q000378:metabolism
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D020112:Rhodamine 123