Biolistic inoculation of gladiolus with cucumber mosaic cucumovirus.
A new method of inoculation of gladiolus with cucumber mosaic virus (CMV) was developed using the Bio-Rad Helios Gene Gun System. This method circumvents the traditional use of aphids to transmit CMV, a virus that is mechanically transmissible to many plant species but only with difficulty to gladiolus. Cartridges containing virus-coated gold microcarriers were prepared and the virus shot into Nicotianabenthamiana leaves and gladiolus corms and cormels. The biolistic procedure successfully transmitted three CMV isolates, two from serogroup I and one from serogroup II. Survival rates of two cultivars of gladiolus cormels and corms in sterile and non-sterile environments were compared. Infection rates of 100% were obtained when as little as 2 microg of virus was used in cartridge preparation. CMV remained viable after the cartridges were stored for many months at 4 degrees C.
Aebig
Joan A
JA
Floral and Nursery Plants Research Unit, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705-2350, USA.
Kamo
Kathryn
K
Hsu
Hei-Ti
HT
D019470:Biolistics / Q000379:methods*; D017799:Cucumovirus / Q000502:physiology*; D027621:Iridaceae / Q000235:genetics / Q000821:virology*; D010316:Particle Size; D010935:Plant Diseases / Q000821:virology*; D014026:Nicotiana / Q000821:virology