title: Plasma proteome of severe acute respiratory syndrome analyzed by two-dimensional gel electrophoresis and mass spectrometry.
abstract: We have investigated the plasma proteome by using 2D gel electrophoresis and MS from patients with severe acute respiratory syndrome (SARS). A complete proteomic analysis was performed on four patients with SARS in different time courses, and a total of 38 differential spots were selected for protein identification. Most of the proteins identified are acute phase proteins, and their presence represents the consequence of serial cascades initiated by SARS-coronavirus infection. There are several proteins that have never been identified in plasma before using 2D gel electrophoresis, among which peroxiredoxin II was chosen for further study by analyzing additional 20 plasma samples from patients with probable and suspected SARS and patients with fever, respectively. The results showed that the level of plasma peroxiredoxin II in patients with SARS is significantly high and could be secreted by T cells. Taken together, our findings indicate that active innate immune responses, along with the oxidation-associated injuries, may play a major role in the pathogenesis of SARS.
authors.affiliation: School of Dentistry, Tri-Service General Hospital, National Defense Medical Center, National Defense University, Taipei 114, Taiwan. jjhc.ndmc@msa.hinet.net
authors.forename: Jenn-Han
authors.initials: JH
authors.lastname: Chen
authors.forename: Yu-Wang
authors.initials: YW
authors.lastname: Chang
authors.forename: Chen-Wen
authors.initials: CW
authors.lastname: Yao
authors.forename: Tzong-Shi
authors.initials: TS
authors.lastname: Chiueh
authors.forename: Su-Chin
authors.initials: SC
authors.lastname: Huang
authors.forename: Ko-Yi
authors.initials: KY
authors.lastname: Chien
authors.forename: An
authors.initials: A
authors.lastname: Chen
authors.forename: Feng-Yee
authors.initials: FY
authors.lastname: Chang
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Chen
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007113:Immunity, Innate; D013058:Mass Spectrometry; D010544:Peroxidases / Q000097:blood / Q000378:metabolism; D054464:Peroxiredoxins; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D045169:Severe Acute Respiratory Syndrome / Q000097:blood*; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D000209:Acute-Phase Proteins; D001798:Blood Proteins; D020543:Proteome; D010544:Peroxidases; D054464:Peroxiredoxins