Age-dependent suppression of SERCA2a mRNA in pediatric atrial myocardium.
Differential expression of sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2a) and phospholamban (PLB) has been shown in heart failure and atrial arrhythmias. We investigated the influence of volume overload and age on their expression in pediatric atrial myocardium. Right atrial specimens from 18 children with volume overloaded right atrium (VO) and 12 patients without overload were studied. Each group was further divided into patients less than and older than 12 months of age. Only in the younger patients SERCA2a was significantly reduced in the VO group. In younger patients PLB mRNA level tended to be lower in VO. The PLB:SERCA protein ratio was significantly reduced in the VO group. Age itself did not influence the SERCA2a and PLB expression, if the hemodynamic overload was not taken into account. This study is the first to show a combined influence of volume overload and age on atrial SERCA2a expression.
Pavlovic
Mladen
M
Division of Pediatric Cardiology, University Children's Hospital, 3010 Berne, Switzerland. mladen.pavlovic@insel.ch <mladen.pavlovic@insel.ch>
Schaller
André
A
Pfammatter
Jean-Pierre
JP
Carrel
Thierry
T
Berdat
Pascal
P
Gallati
Sabina
S
D000293:Adolescent; D000375:Aging / Q000235:genetics*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007223:Infant; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000099211:Phospholamban
D002135:Calcium-Binding Proteins; D000252:Calcium-Transporting ATPases; D012333:RNA, Messenger; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000099211:Phospholamban