title: Proteasome inhibitors induce changes in chromatin structure characteristic of senescent human fibroblasts.
abstract: Inhibitors of proteasome induced premature senescence in normal human fibroblasts. Besides morphological alteration and expression of senescence marker genes, these cells manifested senescence-associated heterochromatic foci under staining of the nuclei with DAPI similar to normally senescent cells. These results suggest that declining ability in protein degradation may be involved in the formation of heterochromatic foci in senescent fibroblasts.
authors.affiliation: Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ukekawa
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Maegawa
authors.forename: Emi
authors.initials: E
authors.lastname: Mizutani
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D000375:Aging / Q000502:physiology; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002843:Chromatin / Q000737:chemistry* / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure*; D006801:Humans; D061988:Proteasome Inhibitors
chemical_list: D002843:Chromatin; D061988:Proteasome Inhibitors