Preparative isolation and purification of psoralen and isopsoralen from Psoralea corylifolia by high-speed counter-current chromatography.
Psoralen and isopsoralen were separated from Psoralea corylifolia by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:5:4.5:5.5, v/v) was used for HSCCC separation, and yielded, from 100 mg of crude extract, 39.6 mg of psoralen and 50.8 mg of isopsoralen each at over 99% purity as determined by high performance liquid chromatography (HPLC). The identification of psoralen and isopsoralen were performed with 1H NMR and 13C NMR.
Liu
Renmin
R
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. renminliu@lctu.edu.cn
Li
Aifeng
A
Sun
Ailing
A
Kong
Lingyi
L
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005363:Ficusin / Q000302:isolation & purification*; D011564:Furocoumarins / Q000302:isolation & purification*; D029907:Psoralea / Q000737:chemistry*
D011564:Furocoumarins; C011659:angelicin; D005363:Ficusin