title: [Treatment of persistent epithelial defects using autologous serum application].
abstract: OBJECTIVE
  To study the impact of topical treatment with autologous serum on the clinical evolution of persistent corneal epithelial defects (PED) and to observe its effect on squamous metaplasia in 7 cases of dry eye.
  
  
  METHODS
  In this prospective, clinical, non-comparative case series study we evaluated a total of 17 eyes (14 patients). We analized the clinical evolution (fluorescein staining, Schirmer's I and II tests and photographic control) of PED in these patients, to whom 20% autologous serum eyedrops were applied for 28 days. Using impression cytology technique (Tseng's method) we evaluated the involution of squamous metaplasia in 7 eyes of 4 patients with ocular cicatricial pemphigoid and dry eye who were treated with autologous serum. The Wilconxon test was used for the statistical treatment of the data.
  
  
  RESULTS
  The epithelial defect healed within 2 weeks in 6 eyes (35.2%). Eight eyes (47%) healed within 2-4 weeks and 3 eyes (17.6%) did not heal after week 4. The duration of PED, before serum therapy was 36 days. Six of seven eyes treated with autologous serum presented an involution of squamous metaplasia 28 days after initiation of the serum drops.
  
  
  CONCLUSIONS
  Autologous serum treatment accelerates PED healing. Autologous serum application causes an involution of squamous metaplasia.
authors.affiliation: Hospital General Universitario de Elche, Alicante, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Alvarado Valero
authors.forename: J J
authors.initials: JJ
authors.lastname: Martínez Toldos
authors.forename: J
authors.initials: J
authors.lastname: Borras Blasco
authors.forename: A
authors.initials: A
authors.lastname: Almiñana Almiñana
authors.forename: J M
authors.initials: JM
authors.lastname: Pérez Ramos
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D003316:Corneal Diseases / Q000209:etiology / Q000628:therapy; D015352:Dry Eye Syndromes / Q000150:complications; D019573:Epithelium, Corneal; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D044967:Serum