title: Nicoletella semolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease.
abstract: Gram-negative, nonmotile bacteria that are catalase, oxidase, and urease positive are regularly isolated from the airways of horses with clinical signs of respiratory disease. On the basis of the findings by a polyphasic approach, we propose that these strains be classified as Nicoletella semolina gen. nov, sp. nov., a new member of the family Pasteurellaceae. N. semolina reduces nitrate to nitrite but is otherwise biochemically inert; this includes the lack of an ability to ferment glucose and other sugars. Growth is fastidious, and the isolates have a distinctive colony morphology, with the colonies being dry and waxy and looking like a semolina particle that can be moved around on an agar plate without losing their shape. DNA-DNA hybridization data and multilocus phylogenetic analysis, including 16S rRNA gene (rDNA), rpoB, and infB sequencing, clearly placed N. semolina as a new genus in the family Pasteurellaceae. In all the phylogenetic trees constructed, N. semolina is on a distinct branch displaying approximately 5% 16S rDNA, approximately 16% rpoB, and approximately 20% infB sequence divergence from its nearest relative within the family Pasteurellaceae. High degrees of conservation of the 16S rDNA (99.8%), rpoB (99.6%), and infB (99.7%) sequences exist within the species, indicating that N. semolina isolates not only are phenotypically homogeneous but also are genetically homogeneous. The type strain of N. semolina is CCUG43639(T) (DSM16380(T)).
authors.affiliation: Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland. peter.kuhnert@vbi.unibe.ch
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
authors.forename: Bozena
authors.initials: B
authors.lastname: Korczak
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
authors.forename: Reto
authors.initials: R
authors.lastname: Straub
authors.forename: Anneliese
authors.initials: A
authors.lastname: Hoops
authors.forename: Patrick
authors.initials: P
authors.lastname: Boerlin
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
authors.forename: Reinier
authors.initials: R
authors.lastname: Mutters
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008171:Lung Diseases / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015661:Pasteurellaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016871:Pasteurellaceae Infections / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S