title: Coexistence of treatment-related MLL cleavage and rearrangement in a child with haemophagocytic lymphohistiocytosis.
abstract: Treatment-related acute myeloid leukaemia (t-AML) is a serious complication of topoisomerase 2 inhibitor therapy and is characterised by the presence of mixed lineage leukaemia (MLL) rearrangement. By molecular tracking, we were able to show that MLL cleavage preceded gene rearrangement by 3 months and before the clinical diagnosis of t-AML in a patient with haemophagocytic lymphohistiocytosis. This is the first report on the sequential detection of the two biomarkers in treatment-related leukaemogenesis.
authors.affiliation: Immunogenetics Laboratory, University of Manchester, Central Manchester and Manchester Children's University Hospitals Trust, Manchester, UK. antng@blueyonder.co.uk
authors.forename: A
authors.initials: A
authors.lastname: Ng
authors.forename: P F
authors.initials: PF
authors.lastname: Ravetto
authors.forename: G M
authors.initials: GM
authors.lastname: Taylor
authors.forename: R F
authors.initials: RF
authors.lastname: Wynn
authors.forename: O B
authors.initials: OB
authors.lastname: Eden
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects*; D015139:Blotting, Southern; D004268:DNA-Binding Proteins / Q000235:genetics*; D003907:Dexamethasone / Q000008:administration & dosage; D020031:Epstein-Barr Virus Infections / Q000150:complications; D005047:Etoposide / Q000009:adverse effects; D015321:Gene Rearrangement; D015616:Histiocytosis, Non-Langerhans-Cell / Q000188:drug therapy* / Q000209:etiology; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D007223:Infant; D015479:Leukemia, Myelomonocytic, Acute / Q000209:etiology*; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage; D051788:Myeloid-Lymphoid Leukemia Protein; D016609:Neoplasms, Second Primary / Q000235:genetics*; D019384:Nucleic Acid Synthesis Inhibitors / Q000009:adverse effects*; D016133:Polymerase Chain Reaction; D011519:Proto-Oncogenes / Q000235:genetics*; D013997:Time Factors; D059005:Topoisomerase II Inhibitors; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C497422:KMT2A protein, human; D019384:Nucleic Acid Synthesis Inhibitors; D059005:Topoisomerase II Inhibitors; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D005047:Etoposide; D003907:Dexamethasone; D011495:Histone-Lysine N-Methyltransferase; D008727:Methotrexate