title: [Study on serum proteome of rat endotoxemia treated by figwort root].
abstract: OBJECTIVE
  To study the serum proteome of rat endotoxemia treated by figwort root (FR).
  
  
  METHODS
  The differences of serum proteome among rats treated with lipopolysaccharide (LPS), FR, LPS + FR and saline respectively were analyzed by two-dimensional electrophoresis (2DE) assay.
  
  
  RESULTS
  The volumes of sixteen serum proteins (xPr) in LPS induced-endotoxemia group were greatly changed compared with those of the control group. Among them, the volumes of xPr 16, 19 were significantly decreased, and the volumes of xPr 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 18, 23 were significantly increased. When treated with FR, the volumes of xPr 1, 6, 7, 8, 9, 11, 12, 14, 18, 23 were significantly decreased, and the volumes of xPr 8, 9, 11, 12, 23, 14 were back to normal level. Two factors statistic analysis showed that FR had interaction with LPS for xPr 1, 5, 8, 10, 11, 12, 18, 19, 20, 21, 22, and FR might be the functional antagonist of LPS. We also observed that the volumes of xPr 10, 13, 15, 20, 21, 22 were found to change significantly only in FR treated group but not in LPS treated group or control group. Interestingly, the volume of xPr 13, 20, 21, 22 were increased and the volume of xPr 10, 15 were decreased.
  
  
  CONCLUSIONS
  The molecular basis of therapeutic effect of FR on endotoxemia might be through the regulation of xPr 1, 6, 7, 8, 9, 11, 12, 14, 18, 23. We can use proteomic techniques to study the molecular mechanisms of diseases treated by functional Chinese herbs and the combination of different herbs is necessary for the treatment of endotoxemia, as FR can not regulated all the changed proteins induced by LPS.
authors.affiliation: Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China. xiewenguang@hotmail.com
authors.forename: Wen-guang
authors.initials: WG
authors.lastname: Xie
authors.forename: Ning-sheng
authors.initials: NS
authors.lastname: Shao
authors.forename: Xiao-chang
authors.initials: XC
authors.lastname: Ma
authors.forename: Qin-xue
authors.initials: QX
authors.lastname: Ding
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Nong-le
authors.initials: NL
authors.lastname: Liu
authors.forename: Yu-shu
authors.initials: YS
authors.lastname: Wei
authors.forename: Hui-xin
authors.initials: HX
authors.lastname: Wang
authors.forename: Ke-ji
authors.initials: KJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D019446:Endotoxemia / Q000097:blood* / Q000139:chemically induced; D007275:Injections, Intravenous; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D032266:Scrophularia / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D020543:Proteome