The ubiquitin ligase XBAT32 regulates lateral root development in Arabidopsis.
Ubiquitin-mediated protein modification plays a key role in many cellular signal transduction pathways. The Arabidopsis gene XBAT32 encodes a protein containing an ankyrin repeat domain at the N-terminal half and a RING finger motif. The XBAT32 protein is capable of ubiquitinating itself. Mutation in XBAT32 causes a number of phenotypes including severe defects in lateral root production and in the expression of the cell division marker CYCB1;1::GUS. The XBAT32 gene is expressed abundantly in the vascular system of the primary root, but not in newly formed lateral root primordia. Treatment with auxin increases the expression of XBAT32 in the primary root and partially rescues the lateral root defect in xbat32-1 mutant plants. Thus, XBAT32 is a novel ubiquitin ligase required for lateral root initiation.
Nodzon
Lisa A
LA
Department of Plant Pathology, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA.
Xu
Wei-Hui
WH
Wang
Yongsheng
Y
Pi
Li-Ya
LY
Chakrabarty
Pranjib K
PK
Song
Wen-Yuan
WY
D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D007210:Indoleacetic Acids / Q000378:metabolism; D008025:Ligases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism*
D029681:Arabidopsis Proteins; C462528:CycB1 protein, Arabidopsis; D019926:Cyclin B; D007210:Indoleacetic Acids; D011993:Recombinant Fusion Proteins; D044767:Ubiquitin-Protein Ligases; C499439:XBAT32 protein, Arabidopsis; D008025:Ligases