title: Nutritional assessment and fate of DNA of soybean meal from roundup ready or conventional soybeans using rats.
abstract: This study was conducted to compare the safety of soybean meal prepared from genetically modified (GM) glyphosate-tolerant (Roundup Ready; RR) soybeans and conventional soybeans. Eighty Sprague-Dawley rats (40 males and 40 females) were randomly allotted to one of four groups according to sex and body weight for a 13-week feeding experiment. The rats were fed corn-based diets containing 60% conventional soybean meal, a mixture of 30% conventional and 30% RR soybean meal, 60% or 90% RR soybean meal. All diets were adjusted to an identical nutrient level except the 90% RR diet. The two soybean meals were similar in chemical analysis and amino acid composition. During the 13-week growth trial, body weight (P < 0.05) and feed intake (P < 0.05) decreased only in rats fed with 90% RR soybean meal at the first week. No treatment-related deaths occurred during the experiment. Gross necropsy findings, haematological or urinalysis values and clinical serum parameters showed no meaningful differences between rats fed the control and RR soybean meals. A 145 bp of cp4 epsps gene specific for the GM constructs from RR soybean meal or a 407 bp of lec gene from endogenous soybean DNA could not be detected in investigated masseter muscle samples. No adverse effects of glyphosate-tolerant soybean meal on rats were seen even at levels as high as 90% of the diet.
authors.affiliation: National Feed Engineering Technology Research Center, China Agricultural University, Beijing, China.
authors.forename: Yuanzhao
authors.initials: Y
authors.lastname: Zhu
authors.forename: Defa
authors.initials: D
authors.lastname: Li
authors.forename: Fenglai
authors.initials: F
authors.lastname: Wang
authors.forename: Jingdong
authors.initials: J
authors.lastname: Yin
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D018744:DNA, Plant / Q000032:analysis* / Q000097:blood / Q000652:urine; D004305:Dose-Response Relationship, Drug; D002149:Energy Intake / Q000187:drug effects; D005260:Female; D008297:Male; D015596:Nutrition Assessment; D030821:Plants, Genetically Modified / Q000009:adverse effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000254:growth & development*; D012449:Safety; D030262:Soybean Proteins / Q000008:administration & dosage* / Q000235:genetics; D013025:Glycine max / Q000235:genetics*; D016482:Urinalysis / Q000662:veterinary; D015430:Weight Gain / Q000187:drug effects
chemical_list: D018744:DNA, Plant; D030262:Soybean Proteins