title: Interaction between the TolC and AcrA proteins of a multidrug efflux system of Escherichia coli.
abstract: This paper provides the biochemical evidence for physical interactions between the outer membrane component, TolC, and the membrane fusion protein component, AcrA, of the major antibiotic efflux pump of Escherichia coli. Cross-linking between TolC and AcrA was independent of the presence of any externally added substrate of the efflux pump or of the pump protein, AcrB. The biochemical demonstration of a TolC-AcrA interaction is consistent with genetic studies in which extragenic suppressors of a mutant TolC strain were found in the acrA gene.
authors.affiliation: Microbiology Graduate Program, Arizona State University, Tempe, AZ 85287-4501, USA.
authors.forename: Fasahath
authors.initials: F
authors.lastname: Husain
authors.forename: Matthew
authors.initials: M
authors.lastname: Humbard
authors.forename: Rajeev
authors.initials: R
authors.lastname: Misra
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013388:Succinimides / Q000494:pharmacology
chemical_list: C083497:AcrA protein, E coli; D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D003432:Cross-Linking Reagents; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D013388:Succinimides; C073126:tolC protein, E coli; D004917:Erythromycin; C011240:dithiobis(succinimidylpropionate)