title: [Establishment and evaluation of the method for removing albumin and IgG in serum proteomics].
abstract: OBJECTIVE
  To establish and evaluate the method for removing albumin and IgG in serum proteomics.
  
  
  METHODS
  Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE).
  
  
  RESULTS
  The method for removing serum albumin and IgG was established, which enabled visualization of low-abundance proteins better in 2-DE map.
  
  
  CONCLUSIONS
  The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. snow110@fimmu.com
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Shou-jun
authors.initials: SJ
authors.lastname: Xu
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Ding
mesh_terms: D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012709:Serum Albumin / Q000302:isolation & purification*
chemical_list: D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin