title: Coupling of fully automated chip electrospray to Fourier transform ion cyclotron resonance mass spectrometry for high-performance glycoscreening and sequencing.
abstract: The NanoMate robot has been coupled to a Fourier transform ion cyclotron resonance (FTICR) mass spectrometer at 9.4 T and implemented for the first time for complex carbohydrate analysis. It was optimized in the negative ion mode to achieve automated sample delivery on the chip along with increased sensitivity, ultra-high resolution and accurate mass determination. A novel bracket has been designed to allow a reliable mounting of the NanoMate to the Apollo electrospray ionization (ESI) source of an APEX II instrument. The notably higher efficiency of ionization for compositional mapping of complex mixtures and feasibility for fragmentation analysis of components by sustained off-resonance irradiation collision-induced tandem mass spectrometry (SORI-CID MS2) has been demonstrated on a glycoconjugate mixture containing O-glycosylated sialylated peptides from urine of a patient suffering from a hereditary N-acetylhexosaminidase deficiency (Schindler's disease), previously analyzed by capillary-based nanoESI-FTICRMS, and of a healthy control person. Due to its potential to generate highly charged ionic species, reduce the in-source fragmentation, increase sensitivity, reproducibility and ionization efficiency, along with the ability to generate a sustained and constant electrospray, this method can be considered as a new platform for advanced glycomics.
authors.affiliation: Institute for Medical Physics and Biophysics, University of Münster, Robert Koch Str. 31, 48149 Münster, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Froesch
authors.forename: Laura M
authors.initials: LM
authors.lastname: Bindila
authors.forename: Gökhan
authors.initials: G
authors.lastname: Baykut
authors.forename: Mark
authors.initials: M
authors.lastname: Allen
authors.forename: Jasna
authors.initials: J
authors.lastname: Peter-Katalinić
authors.forename: Alina D
authors.initials: AD
authors.lastname: Zamfir
mesh_terms: D001331:Automation; D017357:Cyclotrons; D002549:Diffuse Cerebral Sclerosis of Schilder / Q000652:urine; D006020:Glycopeptides / Q000032:analysis / Q000737:chemistry* / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D006020:Glycopeptides