title: Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case.
abstract: Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.
authors.affiliation: Laboratoire de Spectroscopie Moléculaire de Surface, University of Namur, rue de Bruxelles, 61, 5000 Namur, Belgium. laurent.dreesen@fundp.ac.be
authors.forename: Laurent
authors.initials: L
authors.lastname: Dreesen
authors.forename: Yannick
authors.initials: Y
authors.lastname: Sartenaer
authors.forename: Christophe
authors.initials: C
authors.lastname: Humbert
authors.forename: Alaa A
authors.initials: AA
authors.lastname: Mani
authors.forename: Christophe
authors.initials: C
authors.lastname: Méthivier
authors.forename: Claire-Marie
authors.initials: CM
authors.lastname: Pradier
authors.forename: Paul A
authors.initials: PA
authors.lastname: Thiry
authors.forename: André
authors.initials: A
authors.lastname: Peremans
mesh_terms: D001360:Avidin / Q000737:chemistry*; D001665:Binding Sites; D002124:Calcium Fluoride / Q000737:chemistry; D018360:Crystallography, X-Ray; D008024:Ligands; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding / Q000502:physiology; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
chemical_list: D008024:Ligands; D001360:Avidin; C013411:biocytin; D008239:Lysine; D002124:Calcium Fluoride