Cell migration analyses within fibroblast-derived 3-D matrices.
Research in cell biology often is based in tissue culturing cells on artificial substrates, such as plastic or glass. These artificial conditions are prone to distorting findings by persuading cells to adjust to artificial flat rigid surfaces. In contrast, the natural substrate for most cells in living organisms is the extracellular matrix (ECM), which is three-dimensional, complex, and dynamic in its molecular composition, and variable in pliability. Here we show how to measure the rates and directionality of fibroblasts motility within physiologically relevant in vivo-like cell-derived three-dimensional matrices.
Cukierman
Edna
E
Tumor Cell Biology, Basic Science, Fox Chase Cancer Center, Philadelphia, PA, USA.
D016475:3T3 Cells; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002465:Cell Movement / Q000502:physiology*; D003470:Culture Media; D005109:Extracellular Matrix / Q000502:physiology* / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D007202:Indicators and Reagents; D051379:Mice
D003470:Culture Media; D007202:Indicators and Reagents