Effects of Shuanghuangbu on the total protein content and ultrastructure in cultured human periodontal ligament cells.
BACKGROUND
Successful periodontal regeneration depends on the migration, proliferation and differentiation of periodontal ligament cells in periodontal defects. The total protein content and the ultrastructure demonstrate the metabolizability and activity of periodontal ligament cells. This study was conducted to observe the effects of Shuanghuangbu, a mixture of medicinal herbs, on the total protein content and the ultrastructure of human periodontal ligament cells.


METHODS
Periodontal ligament cells were grown to confluence and then cultured in Dulbecco's modified eagle medium (DMEM) supplemented with Shuanghuangbu over the concentration range of 0 to 1000 microg/ml. The total protein content in cultured cells was determined by using Coommasie brilliant blue technique. Periodontal ligament cells were incubated in 0 and 100 microg/ml Shuanghuangbu decoction for 5 days, then observed through transmission electron microscope.


RESULTS
The total protein content of human periodontal ligament cells increased in each experiment group added 10 - 1000 microg/ml Shuanghuangbu respectively, and the effect of 100 microg/ml was excellent. Under the transmission electron microscope, there were more rough endoplasmic reticulums and mitochodrias in the experiment group than those in the control group.


CONCLUSIONS
Shuanghuangbu stimulates the protein synthesis of human periodontal ligament cells and improves human periodontal ligament cells' metabolizability and activity.
Xu
Yan-Zhi
YZ
Department of Stomatology, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050011, China. Xu_yanzhi@163.com
Zou
Hui-Ru
HR
Wang
Xiao-Ling
XL
Liu
Shi-Zheng
SZ
Wang
Yong-Jun
YJ
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D010513:Periodontal Ligament / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D011506:Proteins