title: Role of endogenous macrophage inflammatory protein-2 in regulating fever induced by bacterial endotoxin in normal and immunosuppressed rats.
abstract: During myelosuppressive chemotherapy, Gram-negative bacterial infection with consequent exposure to lipopolysaccharide (LPS) is one of the most important causes of persistent fever. The classical model of the pathogenesis of fever suggests that pro-inflammatory cytokines, produced by leucocytes in the bloodstream in response to exogenous pyrogens such as bacterial LPS, represent the distal mediators of the febrile response. Neutrophils are the first effectors cells and the most prominent leucocyte population involved in acute bacterial infection. Macrophage inflammatory protein (MIP)-2 plays a crucial role in influencing early cell trafficking and neutrophil activation during pathophysiological processes and serves the same chemotactic function as human interleukin-8. In the present study, we investigated the role of MIP-2 in the development of a febrile response induced by LPS in immunocompetent and leukopenic rats. Intraperitoneal injection of LPS in leukopenic rats elicited a biphasic febrile response of rapid onset, the magnitude and duration of which were significantly greater than in immunocompetent animals. The febrile responses to LPS were accompanied by a pronounced induction of serum MIP-2 levels at 1, 2 and 4 h compared with their respective controls. In both normal and leukopenic rats, neutralization of endogenous MIP-2 bioactivity by systemic administration of antirat MIP-2 antibody caused a significant attenuation of the early phase of LPS fever. However, in contrast with normal rats, the second phase of fever was unimpaired by anti-MIP-2 in leukopenic rats. These findings suggest that circulating MIP-2 is involved in the generation of the early phase of LPS fever that contributes to the maintenance of the later phase of fever in immunocompetent, but not leukopenic, rats. Our data support a regulatory role for endogenous MIP-2 in initiating the fever responses to LPS. Furthermore, these results provide evidence that different cellular and humoral mechanisms are implicated in the development of a febrile response triggered by Gram-negative bacterial infections in leukopenic hosts.
authors.affiliation: Research Unit, Laboratory for Clinical and Experimental Pharmacology, Valme University Hospital, Seville, Spain. jminano@us.es
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Miñano
authors.forename: Eva
authors.initials: E
authors.lastname: Tavares
authors.forename: Rosario
authors.initials: R
authors.lastname: Maldonado
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D054426:Chemokine CXCL2; D005334:Fever / Q000139:chemically induced / Q000503:physiopathology*; D007114:Immunization; D007165:Immunosuppression Therapy; D007700:Kinetics; D007958:Leukocyte Count; D007970:Leukopenia / Q000503:physiopathology; D008070:Lipopolysaccharides; D008297:Male; D015846:Monokines / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D054426:Chemokine CXCL2; D008070:Lipopolysaccharides; D015846:Monokines