[Establishment of a method to detect multiple antennae carcinoembryonic antigen (MA-CEA) and MA-CEA level in the sera of patients with malignant tumors].
OBJECTIVE
To establish a rapid quantitative method to detect the serum multiple antennae carcinoembryonic antigen (MA-CEA), and to measure the MA-CEA levels in the sera of patients with malignant tumors.


METHODS
Neuraminidase (NMD) was used to digest the sialic acid at terminals of sugar chains of MA-CEA, and then the datura stramonium agglutinin (DSA) and anti-CEA antibody were used to set up streptavidin-biotin complex (ABC) system EIA (ABC-EIA) for detecting serum MA-CEA levels of 239 patients with carcinomas of lung, stomach, liver, colon and ovary. The serum CEA level was determined by ELISA.


RESULTS
The level of serum MA-CEA was remarkably elevated in the CEA-positive patients with lung cancer. The positive rate of MA-CEA was 14.6% in 75 patients with lung cancers, and 47.3% in 19 CEA-positive patients with lung cancers. The MA-CEA was not detected in the sera of other tumor patients.


CONCLUSIONS
We have developed a method for detection of MA-CEA levels, which lays the foundation for the differential diagnosis of lung cancer and for research on the other multiple antennae tumor markers.
Zhang
Li-jun
LJ
Department of Clinical Laboratory Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. pathol@fmmu.edu.cn
Liu
Shu-lin
SL
Xu
Shun-jun
SJ
D014408:Biomarkers, Tumor / Q000097:blood*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D003110:Colonic Neoplasms / Q000097:blood; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood; D008175:Lung Neoplasms / Q000097:blood*; D009439:Neuraminidase; D010051:Ovarian Neoplasms / Q000097:blood; D013274:Stomach Neoplasms / Q000097:blood
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D009439:Neuraminidase