[Determination of hypoxanthine and xanthine in hippocampus by HPLC method].
OBJECTIVE
A method was established for the determination of hypoxanthine and xanthine in Hippocampus by HPLC.


METHODS
The HPLC system consisted of a Kromasil C18 column (250 mm x 4.6 mm, 5 microm) with methanol-water (2:98, V/V) as the mobile phase. The wavelength of detection was 254 nm.


RESULTS
The linear range of hypoxanthine and xanthine were 29.0-464.0 ng (r = 0.99996) and 24.5-392.0 ng (r = 0.9995) respectively. The average recovery of hypoxanthine was 100.73%, RSD = 1.31% (n = 6). The average recovery of xanthine was 100.54%, RSD = 1.14% (n = 6).


CONCLUSIONS
This method is convenient and the result is accurate. It can be used for quality control of Hippocampus.
Zhao
Xiaoli
X
Nanjing University of Traditional Chinese Medicine, Nanjing 210029.
Cui
Xiaobing
X
Di
Liuqing
L
Li
Wei
W
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019271:Hypoxanthine / Q000032:analysis*; D008419:Materia Medica / Q000737:chemistry*; D010598:Pharmacognosy; D011786:Quality Control; D023701:Smegmamorpha; D019820:Xanthine / Q000032:analysis*
D008419:Materia Medica; D019820:Xanthine; D019271:Hypoxanthine