[The effect of splenic lymphocytes from oral immune tolerance rats on activity of NF-kappaB p65 in glomerular mesangial cells].
OBJECTIVE
To explore the effect of splenic lymphocytes from oral immune tolerance rats on NF-kappaB p65 activity in glomerular mesangial cells.


METHODS
10 female Wistar rats with 6-8 weeks of age were divided randomly into two groups, 5 rats for each group. The rats of one group were given through intragastric feeding with 2 mL Fx1A antigen to induce oral immune tolerance, and the rats of the other group only with PBS (as control group). Two weeks later, the rats were sacrificed, and then their spleens were excised and lymphocytes were separated routinely for antigen-specific lymphocytes reaction. The culture supernatants of splenic lymphocytes from oral immune tolerance rats were prepared. The IL-10 level in the supernatants was detected by sandwich ELISA. After the cultured mesangial cells were stimulated with high dose of insulin, the culture supernatants of rat's splenic lymphocytes were added to insulin-treated mesangial cells. Twenty-four hours later, the NF-kappaB p65 activity in mesangial cells was detected by immunohistochemical staining and sandwich ELISA.


RESULTS
As compared with control group, the proliferation reaction of splenic lymphocytes from oral immune tolerance rats was inhibited obviously, and IL-10 level in splenic lymphocyte culture supernatants from the tolerance rats rose markedly (P<0.01). NF-kappaB p65 activity induced by insulin in mesangial cells was inhibited by culture superatants of splenic lymphocytes from oral immune tolerance rats (P<0.05).


CONCLUSIONS
NF-kappaB p65 activity in mesangial cells may be inhibited by IL-10 secreted by splenic lymphocytes from the oral immune tolerance rats.
Du
Xiao-gang
XG
Department of Nephrology, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China. cxmdxg@sina.com.cn
Gan
Hua
H
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D026541:Heymann Nephritis Antigenic Complex / Q000008:administration & dosage; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology*; D050527:Mesangial Cells / Q000166:cytology* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D051996:Transcription Factor RelA / Q000378:metabolism*
D017077:Culture Media, Conditioned; D026541:Heymann Nephritis Antigenic Complex; D051996:Transcription Factor RelA; D016753:Interleukin-10