title: Cytoprotection by neutral fraction of tannat red wine against oxidative stress-induced cell death.
abstract: Some of the beneficial effects of the Mediterranean diet on human pathologies have been attributed to red wine polyphenols. It has been postulated that the antioxidant activity of the latter would be also responsible for the cytoprotective capacity of red wine that has been reported in a few papers. Nevertheless, red wine shows a complex composition, and the active fraction is not known yet. In this context, the protective capacity of total lyophilized extracts of red wine and anthocyanin, neutral, or acidic fractions, was explored in PC12 cells in culture after a hydrogen peroxide insult. Although all fractions showed high antioxidant activity, only the neutral fraction was cytoprotective. The analysis of this active fraction showed that it was rich in the aglycons quercetin and myricetin as well as the glycosides of kaempferol, isorhamnetin, epicatechin, and catechin, some of which are known to be cytoprotective. This is the first paper to reveal the active fraction of total wine responsible of its cytoprotection.
authors.affiliation: Neurochemistry Department, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, 11610 Montevideo, Uruguay. echever@iibce.edu.uy
authors.forename: Carolina
authors.initials: C
authors.lastname: Echeverry
authors.forename: Fernanda
authors.initials: F
authors.lastname: Blasina
authors.forename: Florencia
authors.initials: F
authors.lastname: Arredondo
authors.forename: Margot
authors.initials: M
authors.lastname: Ferreira
authors.forename: Juan Andrés
authors.initials: JA
authors.lastname: Abin-Carriquiry
authors.forename: Luis
authors.initials: L
authors.lastname: Vasquez
authors.forename: Augusto Alejandro
authors.initials: AA
authors.lastname: Aspillaga
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Diez
authors.forename: Federico
authors.initials: F
authors.lastname: Leighton
authors.forename: Federico
authors.initials: F
authors.lastname: Dajas
mesh_terms: D016923:Cell Death / Q000187:drug effects*; D019610:Cytoprotection / Q000187:drug effects*; D005419:Flavonoids / Q000032:analysis* / Q000494:pharmacology; D018384:Oxidative Stress; D014920:Wine / Q000032:analysis*
chemical_list: D005419:Flavonoids