Elemental maps in human allantochorial placental vessels cells. 4. Isoproterenol and sodium nitroprusside effects.
The ionic channels (particularly, K+ and Ca2+ channels) regulate, via the membrane potential, the ionic distribution into the vascular cells. Micro-particule induced X-ray emission (PIXE) analysis was applied to determine the ionic composition of vascular smooth muscle cells (VSMCs) and of vascular endothelial cells (VECs) in the placental human allantochorial vessels in a physiological medium (Hanks' solution) modified by the addition of a NO donor (sodium nitroprusside, SNP) and of a beta-adrenergic stimulator (isoproterenol, ISO). The addition of SNP or ISO induced no modification of the Na, K, Cl, P, S, Mg and Ca concentrations in VSMCs. In VECs, a same effect was observed except an increase of the Mg concentration with ISO. Theses results indicated a retroactive control (active feedback) of the internal ionic distribution by endothelial factors, ionic channels and exchangers.
Guiet-Bara
A
A
Laboratoire de Physiologie et Physiopathologie, Université P et M Curie, 75252 Paris Cedex 05, France.
Michelet-Habchi
C
C
Barberet
Ph
P
Dutta
R K
RK
Moretto
Ph
P
Bara
M
M
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D049033:Chorioallantoic Membrane / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D007477:Ions / Q000032:analysis* / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology*; D010920:Placenta / Q000098:blood supply* / Q000187:drug effects; D011247:Pregnancy
D000318:Adrenergic beta-Agonists; D007473:Ion Channels; D007477:Ions; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D007545:Isoproterenol