Ameliorative effects of black tea extract on aflatoxin-induced lipid peroxidation in the liver of mice.
We have evaluated the ameliorative effect of black tea extract on aflatoxin-induced lipid peroxidation in the liver of mice. Adult male albino mice were orally administered with 25 and 50 microg of aflatoxin in 0.2 ml olive oil/animal/day for 30 days. Results revealed dose-dependent and significantly (p<0.05) higher lipid peroxidation in the liver of aflatoxin-treated mice than that of vehicle control. As compared with vehicle control, the levels of non-enzymatic antioxidants such as glutathione and ascorbic acid, as well as the enzymatic antioxidants such as superoxide dismutase, glutathione peroxidase and catalase were significantly (p<0.05) lowered in the liver of aflatoxin-treated mice. Oral administration of two percent aqueous black tea extract along with aflatoxin for 30 days (groups 6 and 7) caused significant (p<0.05) amelioration in aflatoxin-induced lipid peroxidation by increasing significantly (p<0.05) the activities of enzymatic (superoxide dismutase, glutathione peroxidase, catalase) and contents of non-enzymatic (glutathione and ascorbic acid) antioxidants in the liver of mice as compared with those given aflatoxin alone (groups 4 and 5). Thus, oral administration of black tea along with aflatoxin significantly (p<0.05) ameliorates aflatoxin-induced lipid peroxidation in the liver of mice.
Choudhary
Anamika
A
Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380 009, India.
Verma
R J
RJ
D000284:Administration, Oral; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
D000348:Aflatoxins; D000975:Antioxidants; D010936:Plant Extracts; D013662:Tea; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid