High permissivity of human HepG2 hepatoma cells for influenza viruses.
Human HepG2 hepatoma cells are highly permissive for influenza virus type A and type B, even without the addition of trypsin, and they exhibit a marked cytopathic effect. This property greatly facilitates the primary isolation of influenza viruses. Virus replication was significantly reduced by the plasmin(ogen)-specific inhibitor tranexamic acid, and this suggests a potential role played by the plasminogen/tissue plasminogen activator complex at the surface of HepG2 cells. This might represent a new approach for study of the interrelations of this complex with influenza viruses.
Ollier
Laurence
L
INSERM U526-Laboratoire de Virologie, Faculté de Médecine, avenue de Valombrose, 06107 Nice cedex 2, France. lefebvre@unice.fr.
Caramella
Anne
A
Giordanengo
Valérie
V
Lefebvre
Jean-Claude
JC
D000818:Animals; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D009980:Influenza A virus / Q000472:pathogenicity* / Q000502:physiology; D009981:Influenza B virus / Q000472:pathogenicity* / Q000502:physiology; D008113:Liver Neoplasms; D010959:Tissue Plasminogen Activator / Q000494:pharmacology; D014779:Virus Replication
D010959:Tissue Plasminogen Activator