title: Cyclic peptides selected by phage display mimic the natural epitope recognized by a monoclonal anti-colicin A antibody.
abstract: A 10-mer random peptide library displayed on filamentous bacteriophage was used to determine the molecular basis of the interaction between the monoclonal anti-colicin A antibody 1C11 and its cognate epitope. Previous studies established that the putative epitope recognized by 1C11 antibody is composed of amino acid residues 19-25 (RGSGPEP) of colicin A. Using the phage display technique it was confirmed that the epitope of 1C11 antibody was indeed restricted to residues 19-25 and the consensus motif RXXXPEP was identified. Shorter consensus sequences (RXXPEP, RXXEP, KXXEP) were also selected. It was also demonstrated that the disulfide bond found in one group of the selected peptides was crucial for 1C11 antibody recognition. It was shown that cyclization of the peptides by disulfide bond formation could result in a structure that mimics the natural epitope of colicin A.
authors.affiliation: The Scripps Research Institute, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Stephane
authors.initials: S
authors.lastname: Coulon
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Metais
authors.forename: Martine
authors.initials: M
authors.lastname: Chartier
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Briand
authors.forename: Daniel
authors.initials: D
authors.lastname: Baty
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003087:Colicins / Q000276:immunology*; D016384:Consensus Sequence; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D018716:Molecular Mimicry; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D003087:Colicins; D000939:Epitopes; D019151:Peptide Library; D010456:Peptides, Cyclic