A neurogenomics approach to gene expression analysis in the developing brain.
Secreted and transmembrane proteins provide critical functions in the signaling networks essential for neurogenesis. We used a genetic signal sequence gene trap approach to isolate 189 genes expressed during development in e16.5 whole head, e16.5 hippocampus and e14.5 cerebellum. Gene ontology programs were used to classify the genes into respective biological processes. Four major classes of biological processes known to be important during development were identified: cell communication, cell physiology processes, metabolism and morphogenesis. We used in situ hybridization to determine the temporal and spatial patterns of gene expression in the developing brain using this set of probes. The results demonstrate that gene expression patterns can highlight potential gene functions in specific brain regions. We propose that combining bioinformatics with the gene expression pattern is an effective strategy to identify genes that may play critical roles during brain development.
Jensen
Patricia
P
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, United States.
Magdaleno
Susan
S
Lehman
Karen M
KM
Rice
Dennis S
DS
Lavallie
Edward R
ER
Collins-Racie
Lisa
L
McCoy
J M
JM
Curran
Tom
T
D000818:Animals; D001921:Brain / Q000196:embryology* / Q000502:physiology*; D018076:DNA, Complementary; D005260:Female; D018507:Gene Expression Regulation, Developmental; D023281:Genomics / Q000379:methods*; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy
D018076:DNA, Complementary