p-Iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias.
BACKGROUND
In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).


METHODS
The frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.


RESULTS
All cases of AML-M7 and AML-M I exhibited integrated light emission greater than 73(10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-MO showed results above the cutoff point.


CONCLUSIONS
This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-MI cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.
da Costa
Miriane
M
Department of Clinical Analysis (LAC), School of Pharmaceutical Sciences, State University of São Paulo (UNESP), Araraquara, SP, Brazil.
Ximenes
Valdecir Farias
VF
Brunetti
Iguatemy Lourenço
IL
Falcão
Roberto Passetto
RP
da Fonseca
Luiz Marcos
LM
D001854:Bone Marrow Cells / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007462:Iodobenzenes / Q000737:chemistry*; D007947:Leukemia, Megakaryoblastic, Acute / Q000175:diagnosis* / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000473:pathology; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry* / Q000378:metabolism; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000473:pathology
D007462:Iodobenzenes; C050181:4-iodophenol; D008165:Luminol; D009195:Peroxidase