title: Evaluation of decontamination methods and growth media for primary isolation of Mycobacterium ulcerans from surgical specimens.
abstract: We evaluated four decontamination methods and one nondecontamination procedure in combination with four egg-based media for the primary isolation of Mycobacterium ulcerans from tissue specimens. With mycobacterial recovery and contamination rates of 75.6 and 2.4%, respectively, the combination of the oxalic acid decontamination method with Lowenstein-Jensen medium supplemented with glycerol yielded the best results.
authors.affiliation: Noguchi Memorial Institute for Medical Research, Legon, Switzerland.
authors.forename: Dorothy
authors.initials: D
authors.lastname: Yeboah-Manu
authors.forename: Thomas
authors.initials: T
authors.lastname: Bodmer
authors.forename: Ernestina
authors.initials: E
authors.lastname: Mensah-Quainoo
authors.forename: Samuel
authors.initials: S
authors.lastname: Owusu
authors.forename: David
authors.initials: D
authors.lastname: Ofori-Adjei
authors.forename: Gerd
authors.initials: G
authors.lastname: Pluschke
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D003666:Decontamination / Q000379:methods*; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology* / Q000601:surgery; D019911:Mycobacterium ulcerans / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification*; D019815:Oxalic Acid / Q000494:pharmacology; D014456:Ulcer / Q000382:microbiology* / Q000601:surgery
chemical_list: D003470:Culture Media; D019815:Oxalic Acid; D005990:Glycerol