title: Crystal structure of human PNP complexed with hypoxanthine and sulfate ion.
abstract: Purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme, which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. Human PNP has been submitted to intensive structure-based design of inhibitors, most of them using low-resolution structures of human PNP. Here we report the crystal structure of human PNP in complex with hypoxanthine, refined to 2.6A resolution. The intermolecular interaction between ligand and PNP is discussed.
authors.affiliation: Programa de Pós-graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Canduri
authors.forename: Valmir
authors.initials: V
authors.lastname: Fadel
authors.forename: Marcio Vinícius Bertacine
authors.initials: MV
authors.lastname: Dias
authors.forename: Luiz Augusto
authors.initials: LA
authors.lastname: Basso
authors.forename: Mário Sérgio
authors.initials: MS
authors.lastname: Palma
authors.forename: Diógenes Santiago
authors.initials: DS
authors.lastname: Santos
authors.forename: Walter Filgueira
authors.initials: WF
authors.lastname: de Azevedo
mesh_terms: D018360:Crystallography, X-Ray; D006801:Humans; D019271:Hypoxanthine / Q000737:chemistry / Q000378:metabolism*; D007477:Ions / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D011487:Protein Conformation; D011683:Purine-Nucleoside Phosphorylase / Q000737:chemistry* / Q000378:metabolism*; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007477:Ions; D008024:Ligands; D013431:Sulfates; D019271:Hypoxanthine; D011683:Purine-Nucleoside Phosphorylase