[Effect of EBV encoded BARF1 gene on malignant transformation of human epithelial cell line HBE].
OBJECTIVE
The BARF(1) (BamHI A right frame1)gene,encoded by Epstein-Barr virus(EBV),may have oncogenic properties,which leaded to immortalization of a monkey kidney epithelial cell line and malignant transformation of human B lymphocytes and murine fibroblasts. This study was designed to evaluate effect of BARF(1) on cell transformation and tumorgenesis on human epithelia and its mechanisms.


METHODS
BARF(1) was amplified from B95-8 cell line by polymerase chain reaction (PCR). Eukaryotic recombinant vector pcDNA(3)-BARF(1) was constructed,and transfected into human bronchial epithelial cell line HBE,and expression of BARF(1) in the transfected cells was detected by Western blot. Growth status,proliferation rate,ability of colony formation in soft agar,and tumorigenicity of HBE cells transfected with BARF(1) were studied.


RESULTS
BARF(1) gene promoted proliferation and transformation of HBE cells. After injected with 1 x 10(7) HBE cells transfected with BARF(1),tumor nodes were observed in 2 of 6 mice at the 10th day.


CONCLUSIONS
BARF(1),as a virus oncogene,may play an important role in malignant transformation of HBE cells.
Song
Li-Bing
LB
Research Department, Cancer Center, Sun Yat-sen University, Guangzhou,Guangdong,510060, P.R.China.
Zen
Mu-Sheng
MS
Zhang
Ling
L
Li
Man-Zhi
MZ
Liao
Wen-Ting
WT
Zheng
Mei-Lian
ML
Wang
Hui-Min
HM
Zeng
Yi-Xin
YX
D000818:Animals; D002051:Burkitt Lymphoma / Q000473:pathology / Q000821:virology; D015197:Carcinogenicity Tests; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D017690:Cell Transplantation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005814:Genes, Viral; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D051379:Mice; D008819:Mice, Nude; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014132:Trachea / Q000166:cytology; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology
C086495:BARF1 protein, Human herpesvirus 4; D011994:Recombinant Proteins; D014764:Viral Proteins