Ellipsometry analysis of the in vitro adsorption of tea polyphenols onto salivary pellicles.
The adsorption of components from black tea and of purified tea polyphenols onto a whole unstimulated salivary pellicle-like protein layer, formed in vitro on hydroxyapatite discs, was studied by in situ ellipsometry. It was found that components from black tea and the purified polyphenols epicatechin-3-gallate (ECG), epigallocatechin-3-gallate (EGCG) and theaflavin readily adsorbed onto the pellicle. Further investigations showed that under the experimental conditions of this study, no black tea- or purified polyphenol-modified pellicles were eluted by either phosphate buffer or sodium dodecyl sulphate rinses. Therefore, black tea and its polyphenol components are indicated to have a profound effect on in vitro pellicle modification. Similar effects were observed for tannic acid.
Joiner
Andrew
A
Unilever Oral Care, Port Sunlight Laboratory, Quarry Road East, Bebington, Wirral, Merseyside CH63 3JW, UK. andrew.joiner@unilever.com
Muller
Dries
D
Elofsson
Ulla M
UM
Arnebrant
Thomas
T
D000327:Adsorption; D000328:Adult; D000975:Antioxidants / Q000493:pharmacokinetics; D044946:Biflavonoids / Q000493:pharmacokinetics; D002021:Buffers; D002392:Catechin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D044622:Dental Pellicle / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D005419:Flavonoids / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D010636:Phenols / Q000493:pharmacokinetics*; D059808:Polyphenols; D012031:Refractometry; D012471:Salivary Proteins and Peptides / Q000493:pharmacokinetics; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013634:Tannins / Q000493:pharmacokinetics; D013662:Tea / Q000737:chemistry*; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors
D000975:Antioxidants; D044946:Biflavonoids; D002021:Buffers; D003902:Detergents; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D012471:Salivary Proteins and Peptides; D013634:Tannins; D013662:Tea; C056068:theaflavin; D012967:Sodium Dodecyl Sulfate; D002392:Catechin; D017886:Durapatite; C062669:epicatechin gallate; C045651:epigallocatechin gallate; D014568:Urokinase-Type Plasminogen Activator