title: [Expressions of early growth response 1 and tissue factor in caerulein-induced acute pancreatitis tissues in rats].
abstract: OBJECTIVE
  To observe the expressions of early growth response 1 (Egr-1) and tissue factor (TF) in rat tissues of acute pancreatitis induced by caerulein and to explore their significance.
  
  
  METHODS
  Pancreatitis was induced in rats by high-dose intraabdominal caerulein injection. The changes of Egr-1 mRNA and protein in pancreas were measured by quantitative PCR and Western blotting, and the localization of Egr-1 protein in acinar cells was visualized by immunohistochemistry. TF mRNA levels were also measured by quantitative PCR. High-dose bombesin-stimulated rats served as the negative control.
  
  
  RESULTS
  Egr-1 mRNA was rapidly increased in the pancreas of rats stimulated by high-dose cearulein, and reached the peak level 30 min after the stimulation, whereas band for peak Egr-1 protein level was visualized by Western blotting till 2 h after stimulation. Immunohistochemistry showed that almost every acinar cell in the pancreas was Egr-1-positive, especially in the nucleus. In line with Egr-1 activation, TF mRNA was detected 1 h after the stimulation and increased steadily within the initial 4 h. Only a small quantity of Egr-1 mRNA expression was observed in bombesin-stimulated rats, in which no Egr-1 protein or TF mRNA were detected.
  
  
  CONCLUSIONS
  Egr-1 mRNA and protein were up-regulated in the early stage of pancreatitis. Egr-1, as a pro-inflammatory transcriptional factor, probably plays an important role in the initiation of acute pancreatitis, and its action might be partially mediated through the up-regulation of TF expression.
authors.affiliation: Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. chenxq@bfbt.com
authors.forename: Xue-qing
authors.initials: XQ
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Yu-gang
authors.initials: YG
authors.lastname: Song
authors.forename: Fa-chao
authors.initials: FC
authors.lastname: Zhi
authors.forename: Lan-bo
authors.initials: LB
authors.lastname: Gong
mesh_terms: D000818:Animals; D002108:Ceruletide; D051766:Early Growth Response Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051766:Early Growth Response Protein 1; D012333:RNA, Messenger; D002108:Ceruletide; D013925:Thromboplastin