title: Simplified method for gene targeting vector construction.
authors.affiliation: University of Freiburg Medical Center, Freiburg, Germany.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Gasperowicz
authors.forename: Mateusz
authors.initials: M
authors.lastname: Kolanczyk
authors.forename: Michael
authors.initials: M
authors.lastname: Stock
authors.forename: Florian
authors.initials: F
authors.lastname: Otto
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D056970:Co-Repressor Proteins; D018390:Gene Targeting / Q000379:methods*; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012097:Repressor Proteins / Q000235:genetics*; D013234:Stem Cells / Q000502:physiology*
chemical_list: D056970:Co-Repressor Proteins; D012097:Repressor Proteins; C434946:Tle1 protein, mouse