[Applying competitive polymerase chain reaction to the detection of hepatitis B virus DNA].
OBJECTIVE
To reduce the rate of accidental false negative result in the HBV DNA PCR test on clinical serum samples.


METHODS
A competitive polymerase chain reaction (C-PCR) was used to decrease the false negative ratio. In the C-PCR, a constructed inner control DNA was added for co-amplification with the HBV target DNA.


RESULTS
In a 20 microl C-PCR system, about 60 to 200 copies of inner control DNA could give apparent co-amplification signal band after electrophoresis on a 2% agarose gel. Five of 120 samples of clinical serum (4.2%) could not be amplified.


CONCLUSIONS
C-PCR has the advantage of yielding information on false negative in the HBV DNA PCR assay of clinical serum samples.
Wang
Ling
L
Lab of Molecule Genetics of Eye Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang
Peng
P
Li
Shuang-qing
SQ
Xu
Shu-hui
SH
Cao
Gui-qun
GQ
Zhang
Fa-qiang
FQ
Zhang
Mei-xia
MX
Chen
Qing-ying
QY
Xia
Qing-jie
QJ
Liu
Kai
K
Tang
Fang
F
Zhang
Yuan-zheng
YZ
D004279:DNA, Viral / Q000097:blood*; D005188:False Negative Reactions; D006509:Hepatitis B / Q000175:diagnosis* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D004279:DNA, Viral