Printed covalent glycan array for ligand profiling of diverse glycan binding proteins.
Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.
Blixt
Ola
O
Glycan Synthesis and Protein Expression Core-D, Consortium for Functional Glycomics, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. olablixt@scripps.edu
Head
Steve
S
Mondala
Tony
T
Scanlan
Christopher
C
Huflejt
Margaret E
ME
Alvarez
Richard
R
Bryan
Marian C
MC
Fazio
Fabio
F
Calarese
Daniel
D
Stevens
James
J
Razi
Nahid
N
Stevens
David J
DJ
Skehel
John J
JJ
van Die
Irma
I
Burton
Dennis R
DR
Wilson
Ian A
IA
Cummings
Richard
R
Bovin
Nicolai
N
Wong
Chi-Huey
CH
Paulson
James C
JC
D002240:Carbohydrate Sequence; D003432:Cross-Linking Reagents; D008024:Ligands; D011134:Polysaccharides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012371:Robotics
D003432:Cross-Linking Reagents; D008024:Ligands; D011134:Polysaccharides; D011506:Proteins