title: [Applying competitive polymerase chain reaction to the detection of hepatitis B virus DNA].
abstract: OBJECTIVE
  To reduce the rate of accidental false negative result in the HBV DNA PCR test on clinical serum samples.
  
  
  METHODS
  A competitive polymerase chain reaction (C-PCR) was used to decrease the false negative ratio. In the C-PCR, a constructed inner control DNA was added for co-amplification with the HBV target DNA.
  
  
  RESULTS
  In a 20 microl C-PCR system, about 60 to 200 copies of inner control DNA could give apparent co-amplification signal band after electrophoresis on a 2% agarose gel. Five of 120 samples of clinical serum (4.2%) could not be amplified.
  
  
  CONCLUSIONS
  C-PCR has the advantage of yielding information on false negative in the HBV DNA PCR assay of clinical serum samples.
authors.affiliation: Lab of Molecule Genetics of Eye Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.forename: Shuang-qing
authors.initials: SQ
authors.lastname: Li
authors.forename: Shu-hui
authors.initials: SH
authors.lastname: Xu
authors.forename: Gui-qun
authors.initials: GQ
authors.lastname: Cao
authors.forename: Fa-qiang
authors.initials: FQ
authors.lastname: Zhang
authors.forename: Mei-xia
authors.initials: MX
authors.lastname: Zhang
authors.forename: Qing-ying
authors.initials: QY
authors.lastname: Chen
authors.forename: Qing-jie
authors.initials: QJ
authors.lastname: Xia
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Tang
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D004279:DNA, Viral / Q000097:blood*; D005188:False Negative Reactions; D006509:Hepatitis B / Q000175:diagnosis* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral