Cryopreservation with hydroxyethylstarch (HES) + dimethylsulfoxide (DMSO) gives better results than DMSO alone.
This study compares the cryopreservation of PBSC by means of HES 3% + DMSO 5% ina mechanical freezer with the cryopreservation of PBSC by means of DMSO 10% in an automated rate controlled freezer. The cells harvested from the same patient were divided in two equal parts and frozen according to both methods. Cryopreservation with HES +DMSO is better than DMSO alone in terms of viability (p<0.001) and Granulocyte Macrophage Colony Forming Unit (GM-CFU) activity (p<0.05). The freezing curves differ essentially in the slope down after the surfusion peak. However, surfusion peaks are similar in both conditions. The relevance of mechanical freezing is the saving of time and the safety of such a procedure.
Clapisson
Gilles
G
Centre Leon Berard, Lyon Cedex 08, France.
Salinas
Cecile
C
Malacher
Philippe
P
Michallet
Mauricette
M
Philip
Irene
I
Philip
Thierry
T
D000971:Antineoplastic Combined Chemotherapy Protocols; D015169:Colony Count, Microbial; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D006412:Hematopoietic Stem Cells; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives
D003451:Cryoprotective Agents; D006895:Hydroxyethyl Starch Derivatives; D004121:Dimethyl Sulfoxide