Free radicals scavenging efficiency of a few naturally occurring flavonoids: a comparative study.
The interaction of antiperoxidative flavonoids artocarpin (AR), cycloartocarpin (CAR), dalspinin (DP), dalspinosin (DPO), and dalspinin-7-O-galactoside (DPG) with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(.+)) and O2(-.) was studied in phosphate buffer (pH 7.4). The ability of these compounds to inhibit lipid peroxidation and DNA scission was also investigated. The radical scavenging efficiency of flavonoids is demonstrated by the reduction of nitrogen-centered radical cation (ABTS(.+)). The reduction of ABTS(.+) follows the order quercetin > morin > Trolox > AR > DPO > CAR > DP. Inhibition of lipid peroxidation was studied by following Mb(IV) reduction, induced by lipid, arachidonic acid. These results are compared with those obtained for well-known antioxidants such as quercetin, morin, and Trolox. The structure-activity relationships between chemical structures of the flavonoids and their radical scavenging activities are anlayzed. The scavenging of O2(-.), inhibition of lipid peroxidation, and DNA damage depend on the oxidation potential of the flavonoids. The possible mechanism for radical scavenging activities of flavonoids in relation to their structure is also outlined.
Rajendran
Muthia
M
Department of Chemistry, NMSSVN College, Madurai-625 019, Tamilnadu, India, Department of Industrial Chemistry, Alagappa University, Karaikudi-630 003, Tamilnadu, India.
Manisankar
Paramasivam
P
Gandhidasan
Rathinasamy
R
Murugesan
Ramachandran
R
D000975:Antioxidants / Q000494:pharmacology; D052160:Benzothiazoles; D004249:DNA Damage / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000494:pharmacology; D037121:Plant Lectins / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013451:Sulfonic Acids / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D052160:Benzothiazoles; D005419:Flavonoids; D016166:Free Radical Scavengers; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; D013451:Sulfonic Acids; C090683:artocarpin lectin; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid