Rapid discrimination between human immunodeficiency virus type 2 groups A and B by real-time PCR.
We studied the feasibility of genotyping human immunodeficiency virus (HIV) type 2 groups A and B by real-time PCR. Two group-specific PCRs were developed. Real-time genotyping of 22 samples of genotype A, 10 samples of genotype B, and the isolate of new group H were compared to genotyping by sequencing and phylogeny. The group-specific PCRs specifically identified 84.3% of group A or B samples; isolate H was not detected. This method allowed rapid and specific discrimination between HIV-2 groups A and B and could be a useful tool for molecular epidemiological studies.
Plantier
Jean-Christophe
JC
Unité de Virologie, laboratoire associé au Centre National de Référence du VIH, Equipe d'Accueil 2656, CHU Charles Nicolle, 1 rue de Germont, 76031 Rouen, France. Jean-Christophe.Plantier@univ-rouen.fr
Gueudin
Marie
M
de Oliveira
Fabienne
F
Damond
Florence
F
Lemée
Véronique
V
Brun-Vézinet
Françoise
F
Simon
François
F
D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015498:HIV-2 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors