[Effect of ginsenoside Rg1 on the influence of neprilysin expression induced by LPS in BT325 cell line].
OBJECTIVE
To investigate the effect of ginsenoside Rg1 on the influence of neprilysin expression induced by LPS in BT325 cell line.


METHODS
MTT was used to measure the levels of the survival rate of BT325 cultured with ginsenoside Rg1 and LPS in different concentrations. The expression of NEP was measured by RT-PCR.


RESULTS
The survival rate of BT325 was obviously inhibited by LPS, and the expression of NEP was decreased. The survival rate of BT325 was obviously raised by ginsenoside Rg1, and the expression of NEP was increased.


CONCLUSIONS
LPS can cause cell damage, and decrease the expression of NEP. Ginsenoside Rg1 can exert neuroprotective properties which protects BT325 cell line from the cell toxicity of LPS.
Luo
Huan-min
HM
Neuropharmacological Research Laboratory, College of Pharmacy, Jinan University, Guangzhou 510632, China. tlhm@jnu.edu.cn
Deng
Hui
H
Huang
Feng
F
Xiao
Fei
F
Li
Xiao-guang
XG
Zhang
Pei-fen
PF
Gao
Qin
Q
Weng
Wen
W
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008070:Lipopolysaccharides; D015260:Neprilysin / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D015260:Neprilysin; C035054:ginsenoside Rg1