DNA-loaded biodegradable microparticles as vaccine delivery systems and their interaction with dendritic cells.
This paper provides a review of the role of dendritic cells (DC) in microparticle-mediated immune response and the advantages of associating DNA to microparticles in order to increase the potency of DNA vaccination in vivo. To begin with, different methods for the preparation of DNA-loaded microparticle with poly(lactide) (PLA)/poly(lactide-co-glycolide) (PLGA) polymers are presented. Further, the effects of DNA-loaded microparticles on DC in vitro are extensively examined including transfection and stimulation of DC, a key feature of the immune response. Finally, in vivo tracking of DNA-loaded microparticles and induction of immune responses upon DNA-loaded microparticle administration in different animal models and with various routes of administration are reviewed.
Jilek
Samantha
S
Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology Zurich (ETH), Winterthurerstrerstrasse 190, CH-8057 Zurich, Switzerland.
Merkle
Hans P
HP
Walter
Elke
E
D000327:Adsorption; D000818:Animals; D001673:Biodegradation, Environmental; D003713:Dendritic Cells / Q000502:physiology*; D016503:Drug Delivery Systems; D006801:Humans; D007108:Immune Tolerance; D008863:Microspheres; D010587:Phagocytosis; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage*
D019444:Vaccines, DNA