Oxidative DNA cleavage by free and oligonucleotide-conjugated O-bromobenzoic acid in the presence of copper(II) ions.
o-Bromobenzoic acid was found to promote copper-dependent reactive oxygen species formation from molecular oxygen, resulting in DNA base modification and backbone cleavage. The oligonucleotide conjugate bearing 5-(4'-aminopropyl-sulfomoyl)-2-bromobenzoic acid as a reactive group was synthesized and DNA cleavage activity of this oligonucleotide conjugate was tested on a model deoxyoligonucleotide.
Koval
O A
OA
Laboratory of Nucleic Acid Biochemistry, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. o.koval@niboch.nsc.ru
Oleinikova
S B
SB
Litvak
V V
VV
Vlassov
V V
VV
Chernolovskaya
E L
EL
D001483:Base Sequence; D001970:Bromobenzoates / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry*; D010084:Oxidation-Reduction
D001970:Bromobenzoates; D009841:Oligonucleotides; D003300:Copper; D004247:DNA