title: Modification of physicochemical properties of actin filaments suppresses cell fragmentation in the execution phase of staurosporine-induced apoptotic processes.
abstract: Effects of jasplakinolide (JSP), a stabilizer of F-actin, and latrunculin A (LTA), a destabilizer of F-actin, on a series of events occurring in the execution phase of staurosporine (STS)-induced apoptotic processes were studied using human osteosarcoma 143B cells. Time-dependent apparent increases of the population of cells with collapsed membrane potential of mitochondria (Delta Psi(m)) caused by STS treatment were not due to actual decreases in the Delta Psi(m) per cell, but due to the fragmentation of cells resulting in decreases in the number of active mitochondria per cell. Decreases in the Delta Psi(m) in fragmented cells occurred late in the execution phase. Both JSP and LAT failed to prevent STS-induced release of cytochrome c from mitochondria followed by the activation of caspases 3 and 9, the cleavage of poly (ADP-ribose) polymerase (PARP) and apoptotic nuclear fragmentation. However, both drugs prevented STS-induced apoptotic cell fragmentation and decreases in the Delta Psi(m). These results indicate that physicochemical states of actin filaments play a certain role in the execution phase of STS-induced apoptotic processes.
authors.affiliation: Department of Cell Biology and Molecular Pathology, Medical University of Gdańsk, ul. Debinki 1, 80-210 Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Majczak
authors.forename: Mariusz
authors.initials: M
authors.lastname: Karbowski
authors.forename: Marcin
authors.initials: M
authors.lastname: Kamiński
authors.forename: Makoto
authors.initials: M
authors.lastname: Masaoka
authors.forename: Chieko
authors.initials: C
authors.lastname: Kurono
authors.forename: Edyta
authors.initials: E
authors.lastname: Niemczyk
authors.forename: Jakub
authors.initials: J
authors.lastname: Kedzior
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Soji
authors.forename: Dorota
authors.initials: D
authors.lastname: Knap
authors.forename: Anna
authors.initials: A
authors.lastname: Hallmann
authors.forename: Takashi
authors.initials: T
authors.lastname: Wakabayashi
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D047630:Depsipeptides / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D008564:Membrane Potentials / Q000187:drug effects; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D019311:Staurosporine / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines
chemical_list: D000970:Antineoplastic Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D047630:Depsipeptides; D004791:Enzyme Inhibitors; D013844:Thiazoles; D053778:Thiazolidines; C057531:jasplakinolide; D020169:Caspases; D019311:Staurosporine; C037067:latrunculin A