[The protective effect of dachuanxiongwan on the rats with focal cerebral ischemia and reperfusion].
OBJECTIVE
Studying the protective effect of dachuanxiongwan (DCXW) on the rats with focal cerebral ischemia and reperfusion.


METHODS
The ischemia and reperfusion model was established by blocking the rat's middle cerebral artery with a pre-set nylon thread and removing it from the internal carotid artery 2 hours after occlusion. 20 healthy male Sprague-Dawley rats following middle cerebral artery occlusion (MCAO) were randomly divided into two groups: Group A, transient ischemia, for control; Group B, transient ischemia treated with DCXW via gavage, 4.13 g/kg x d, twice a day for 3 days, while group A being given the same volume of saline. Seventy-two hours after reperfusion, the neurological function of all rats was evaluated according to Longa, and the rats were sacrificed. All the samples of cerebrum were processed and embedded in paraffin and cut into sections. The sections were stained with Hematoxylin & Eosin to measure the infarction volume and evaluate the effects of DCXW.


RESULTS
The grade of neurological function of the treatment group (1.5+/-0.71) was lower than that of the control group (2.3+/-0.82), there was a statistically significant difference between the two groups (P=0.032); the infarction volume of the treatment group was smaller than that of the control group, there was a statistically significant difference between the two groups (P=0.000).


CONCLUSIONS
The findings suggest that DCXW has a protective effect on the cerebrum undergoing ischemia and reperfusion.
He
Li
L
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Zhi-jian
ZJ
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents