Molecular signature associated with plasticity of bone marrow cell under persistent liver damage by self-organizing-map-based gene expression.
The mechanism that regulates the plasticity of bone marrow cells (BMCs) into hepatocytes is poorly understood. We developed a green fluorescent protein/carbon tetrachloride model to find that BMC transplantation recovered liver damage. Serum albumin level and liver fibrosis were recovered by BMC transplantation. To understand the mechanism, we used DNA-chip technology to profile the change of transient gene expression before and after BMC transplantation. On the basis of gene expression with self-organizing map using specific equation, genes were classified into 153 clusters. The information is useful to understand the dramatic gene activation during the process of the plasticity of BMC.
Omori
Kaoru
K
Department of Molecular Science and Applied Medicine (Gastroenterology and Hepatology), Yamaguchi University School of Medicine, Minami Kogushi 1-1-1, Ube, Yamaguchi 755 8505, Japan.
Terai
Shuji
S
Ishikawa
Tsuyoshi
T
Aoyama
Kouji
K
Sakaida
Isao
I
Nishina
Hiroshi
H
Shinoda
Koh
K
Uchimura
Shunji
S
Hamamoto
Yoshihiko
Y
Okita
Kiwamu
K
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002251:Carbon Tetrachloride / Q000494:pharmacology / Q000633:toxicity; D005260:Female; D005355:Fibrosis / Q000139:chemically induced / Q000628:therapy; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D015533:Transcriptional Activation
D049452:Green Fluorescent Proteins; D002251:Carbon Tetrachloride