A novel neurotrophic peptide: APP63-73.
The objective of this study was to find out which N-terminal segment/s of amyloid precursor protein (APP) has any neurotrophic properties, since soluble APP-alpha (sAPP-alpha) has neurotrophic effects. We investigated neurotrophic properties of eight peptide segments of N-terminal APP. The APP63-73 was able to enhance neuronal growth; augment axonal and cell body growth in human neuroblastoma cell line, SH-SY5Y. Neurotrophic effects of chronic APP63-73 treatment were assessed in vivo using streptozotocin-induced diabetes and ovariectomized rats. Thus, this study demonstrated that APP63-73 peptide has neurotrophic effects both in vivo and in vitro.
Wang
Rong
R
Neuro-Biochemistry Laboratory, Beijing Xuan-Wu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
Zhang
Jing-Yan
JY
Yang
Fang
F
Ji
Zhi-Juan
ZJ
Chakraborty
Goutam
G
Sheng
Shu-Li
SL
D016564:Amyloid beta-Protein Precursor / Q000138:chemical synthesis / Q000494:pharmacology* / Q000627:therapeutic use; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002452:Cell Count / Q000379:methods; D045744:Cell Line, Tumor; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009414:Nerve Growth Factors / Q000138:chemical synthesis / Q000494:pharmacology* / Q000627:therapeutic use; D009447:Neuroblastoma; D010052:Ovariectomy / Q000379:methods; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology / Q000627:therapeutic use; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
C500314:APP63-73; D016564:Amyloid beta-Protein Precursor; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D010455:Peptides; D013778:Tetrazolium Salts; D013844:Thiazoles; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue