Assessing problems of organic hydroperoxide-induced chemiluminescence related to biological/medical samples.
The supernatants obtained from mammalian tissue homogenates, blood serum or other body fluids, and supplemented with organic hydroperoxide show a multiplicity of light-emitting reactions. The low-level chemiluminescence of biological samples denotes that after hydroperoxide-induction electronically excited species are produced. The light-emission measured by photomultipliers is related to (i) the geometrical location of the induced sample, (ii) light gathering capacity of the phototubes, and (iii) both the quantity and concentration of samples and reactants. The fewest blood contamination of biological samples leads to an increased photoemission. The very high sensitivity of light detectors requires stable measuring and sampling conditions and peculiarly cautious evaluation of the reactions.
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Bioanalytical Institute, Medical Faculty, University of Pécs, Szigeti út 12, 7624, Pécs, Hungary. bela.torok@aok.pte.hu
D016477:Artifacts; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000494:pharmacology*; D008163:Luminescent Measurements / Q000379:methods*; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide