title: [Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui].
abstract: OBJECTIVE
  To select the sodium chloride tolerant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment.
  
  
  METHODS
  Calli were induced from the leaves of C. morifolium. The calli were treated with 0.2% and 0.5% EMS, respectively. After 15 days' culture, the calli were transplanted to selection media with 0.5%, 1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out.
  
  
  CONCLUSIONS
  After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed.
authors.affiliation: Department of Biology, Huaibei Coal Industry Teachers' College, Huaibei 235000, China. xuejp2000@yahoo.com.cn
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Ai-ming
authors.initials: AM
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
mesh_terms: D031202:Chrysanthemum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D009154:Mutation; D018515:Plant Leaves / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D012965:Sodium Chloride