[Role of PD153035 in the induction of apoptosis of XG-1 myeloma cell line].
OBJECTIVE
To study the role of epidermal growth factor receptor (EGFR) in the proliferation and survival of human myeloma cells.


METHODS
The inhibitor of EGFR, PD153035, was used to block the signal transduction of EGFR. The proliferation and apoptosis of myeloma cell line, XG-1, were detected by 3H-TCR incorporation assay and Annexin V staining analysis, respectively. The phosphatation of STAT3, the key activate signal to the myeloma cell proliferation, was detected with Western blot.


RESULTS
PD153035 decreased the proliferation of XG-1 and induced an obvious apoptosis in XG-1. The phosphatation of STAT3 induced by HB-EGF but not by IL-6 was blocked by PD153035.


CONCLUSIONS
The proliferation and survival of myeloma cells may be suppressed by PD153035 due to the blockage of phosphatation of STAT3 induced by the activation of EGFR.
He
Xiao-yan
XY
Department of Immunology, Peking University Health Science Center, Beijing 100083, China.
Huang
Jian-an
JA
Xie
Wei
W
Jiang
Ling-yu
LY
Wang
Yue-dan
YD
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000378:metabolism; D066246:ErbB Receptors / Q000037:antagonists & inhibitors*; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D011799:Quinazolines / Q000494:pharmacology*; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism
D000970:Antineoplastic Agents; D004268:DNA-Binding Proteins; D011799:Quinazolines; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators; D066246:ErbB Receptors; C088860:4-((3-bromophenyl)amino)-6,7-dimethoxyquinazoline