title: The effect of methylenetetrahydrofolate reductase polymorphism C677T on cervical cancer in Korean women.
abstract: OBJECTIVE
  Methylenetetrahydrofolate reductase (MTHFR) gene is associated with DNA hypomethylation, an established hallmark of human cancer cells. The aim of this study was to examine the effect of MTHFR polymorphism C677T on cervical carcinogenesis in the context of other environmental factors, such as smoking, parity, and age at the first intercourse.
  
  
  METHODS
  The study subjects were patients who were pathologically diagnosed with cervical neoplasia and who had a positive result for human papillomavirus (N = 462), and they were compared to normal healthy women as normal controls (N = 454). Genotypes of the patients and control samples were assayed by single base primer extension assay using SNapShot assay kit.
  
  
  RESULTS
  Compared with MTHFR C/C, the odds ratio (95% confidence interval) for MTHFR T/T was 1.4 (0.9-2.3) for invasive cervical cancer and 1.3 (0.8-2.3) for cervical intraepithelial neoplasia (CIN) II or III. The risks for invasive cervical cancer were higher with less than 40 years old at diagnosis (2.1, 1.0-4.3), than with over 40 years old at diagnosis (1.2, 0.7-2.2). Current smoking women with early onset with MTHFR T/T had a 4.7 (0.6-36.2) times higher risk of cervical cancer. The risks of MTHFR T/T or C/T also increased for women with an early age of first intercourse or for women with two or more children, as compared with MTHFR C/C.
  
  
  CONCLUSIONS
  Polymorphisms of MTHFR are associated with a higher risk of developing cervical cancer, and in particular for an early onset of cervical carcinogenesis.
authors.affiliation: Department of Preventive Medicine and Public Health, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Sull
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Jee
authors.forename: Sangwook
authors.initials: S
authors.lastname: Yi
authors.forename: Jong Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Jong Sup
authors.initials: JS
authors.lastname: Park
authors.forename: Sook
authors.initials: S
authors.lastname: Kim
authors.forename: Heechoul
authors.initials: H
authors.lastname: Ohrr
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000150:complications / Q000201:enzymology; D011110:Polymorphism, Genetic; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000201:enzymology / Q000235:genetics / Q000821:virology
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)