Crystallization of parasporin-2, a Bacillus thuringiensis crystal protein with selective cytocidal activity against human cells.
Bacillus thuringiensis is a valuable source of protein toxins that are specifically effective against certain insects and worms but harmless to mammals. In contrast, a protein toxin obtained from B. thuringiensis strain A1547, designated parasporin-2, is not insecticidal but has a strong cytocidal activity against human cells with markedly divergent target specificity. The 37 kDa inactive protein is proteolytically activated to a 30 kDa active form. The active form of the recombinant protein toxin was crystallized in the presence of ethylene glycol and polyethylene glycol 8000 at neutral pH. The crystals belong to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 134.37, c = 121.24 A. Diffraction data from a native crystal were collected to 2.75 A resolution using a synchrotron-radiation source.
Akiba
Toshihiko
T
Biological Information Research Center, AIST, Tsukuba, Ibaraki 305-8566, Japan.
Abe
Yuichi
Y
Kitada
Sakae
S
Kusaka
Yoshitomo
Y
Ito
Akio
A
Ichimatsu
Tokio
T
Katayama
Hideki
H
Akao
Tetsuyuki
T
Higuchi
Kazuhiko
K
Mizuki
Eiichi
E
Ohba
Michio
M
Kanai
Ryuta
R
Harata
Kazuaki
K
D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D004731:Endotoxins / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008715:Methionine / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014961:X-Ray Diffraction
D001427:Bacterial Toxins; D004731:Endotoxins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011506:Proteins; D011994:Recombinant Proteins; C412914:parasporin; D011092:Polyethylene Glycols; D006639:Histidine; D008715:Methionine