title: Expression, purification and preliminary crystallographic characterization of a novel segment from the neurofibromatosis type 1 protein.
abstract: Neurofibromin (MW 320 kDa) is the protein responsible for the pathogenesis of neurofibromatosis type 1 (NF1), one of the most common genetic diseases worldwide. The neurofibromin GAP-related domain (GRD, MW 38 kDa) possess a Ras-specific GTPase-activating protein property, which is at present its only clear biochemical function. This article describes the study of the bacterial production and preliminary X-ray crystallographic analysis of a neurofibromin fragment located at the C-terminal end of the GRD, which contains a region reported to be homologous to the yeast Sec14p lipid exchange protein. Of the three crystal variants obtained, a tetragonal form diffracted to a resolution of at least 2.3 A.
authors.affiliation: European Molecular Biology Laboratory, Structural and Computational Biology Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: Fabien
authors.initials: F
authors.lastname: Bonneau
authors.forename: Igor
authors.initials: I
authors.lastname: D'Angelo
authors.forename: Stefan
authors.initials: S
authors.lastname: Welti
authors.forename: Gunter
authors.initials: G
authors.lastname: Stier
authors.forename: Jari
authors.initials: J
authors.lastname: Ylänne
authors.forename: Klaus
authors.initials: K
authors.lastname: Scheffzek
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004330:Drosophila / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D008055:Lipids / Q000737:chemistry; D025542:Neurofibromin 1 / Q000737:chemistry*; D049294:Phospholipid Transfer Proteins / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry
chemical_list: D008055:Lipids; D025542:Neurofibromin 1; D049294:Phospholipid Transfer Proteins; C483438:SEC14 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins