Expression changes of the mRNA of Alzheimer's disease related factors in the permanent ischemic rat brain.
The rat with permanent occlusion of the bilateral common carotid arteries (2VO) is useful model for the study of dementia. The expression changes of amyloid precursor protein (APP), secretase, alpha7 nicotinic acetylcholine receptor (alpha7NicR) and acetylcholine esterase (AChE), which are involved in Alzheimer's disease, were examined by quantitative RT/PCR in this model rat brain. The expression of APP, alpha7NicR and secretase were increased 4 d after 2VO. The alpha7NicR level at 2 d after operation already tended to increase. These result suggest that alpha7NicR expression was enhanced at early stage of brain ischemia. Using this model to find drugs which regulate the alpha7NicR expression will be useful to assay the materials with anti-dementive effect.
Tohda
Michihisa
M
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan. tohdam@ms.toyama-mpu.ac.jp
Suwanakitch
Prawpan
P
Jeenapongsa
Rattima
R
Hayashi
Hisae
H
Watanabe
Hiroshi
H
Matsumoto
Kinzo
K
D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger