Alcohol-mediated error-prone PCR.
The effect of urea, isopropanol, propan-1-ol, and butan-1-ol on PCR using three different DNA polymerases was investigated. In the presence of these agents, polymerases were active as expected up to a critical concentration where they became progressively inhibited. Critical concentrations of alcohols generally increased with thermoresistance of the polymerases and decreased with the hydrophobicity of the alcohols. These results indicate that an important aspect of the inhibition involved conformational loosening due to a decrease in the hydrophobic effect. A mutagenic effect occurred with Vent(r) (exo-) DNA polymerase in the presence of 7.0 to 8.0% v/v propan-1-ol, affording mutation frequencies of up to 9.8 x 10(-3) mutation/bp/PCR. Under these conditions the preferential replacement of Gs and Cs was observed, in opposition to standard error-prone PCR that favors replacement of As and Ts. Comparison of various PCR conditions indicates that propanol and MnCl2 have different modes of action, and that the decrease in fidelity promoted by propanol is due to a finely tuned partial destabilization of the polymerase. The PCR conditions developed in this study provide a useful alternative for targeting different sequence space for directed evolution experiments.
Claveau
S
S
Groupe de Recherche en Energie et Information Biomoléculaires, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Canada.
Sasseville
M
M
Beauregard
M
M
D000433:1-Propanol / Q000737:chemistry*; D019840:2-Propanol / Q000737:chemistry*; D001483:Base Sequence; D000440:Butanols / Q000737:chemistry*; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D014508:Urea / Q000737:chemistry
D000440:Butanols; D017931:DNA Primers; D014508:Urea; D000433:1-Propanol; D019840:2-Propanol