title: Rapid discrimination between human immunodeficiency virus type 2 groups A and B by real-time PCR.
abstract: We studied the feasibility of genotyping human immunodeficiency virus (HIV) type 2 groups A and B by real-time PCR. Two group-specific PCRs were developed. Real-time genotyping of 22 samples of genotype A, 10 samples of genotype B, and the isolate of new group H were compared to genotyping by sequencing and phylogeny. The group-specific PCRs specifically identified 84.3% of group A or B samples; isolate H was not detected. This method allowed rapid and specific discrimination between HIV-2 groups A and B and could be a useful tool for molecular epidemiological studies.
authors.affiliation: Unité de Virologie, laboratoire associé au Centre National de Référence du VIH, Equipe d'Accueil 2656, CHU Charles Nicolle, 1 rue de Germont, 76031 Rouen, France. Jean-Christophe.Plantier@univ-rouen.fr
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Plantier
authors.forename: Marie
authors.initials: M
authors.lastname: Gueudin
authors.forename: Fabienne
authors.initials: F
authors.lastname: de Oliveira
authors.forename: Florence
authors.initials: F
authors.lastname: Damond
authors.forename: Véronique
authors.initials: V
authors.lastname: Lemée
authors.forename: Françoise
authors.initials: F
authors.lastname: Brun-Vézinet
authors.forename: François
authors.initials: F
authors.lastname: Simon
mesh_terms: D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015498:HIV-2 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors