title: Simple high-performance liquid chromatographic method for determination of atropine and obidoxime in a parenteral injection device.
abstract: Atropine and obidoxime in a parenteral injection device are determined by simple HPLC method simultaneously without any pretreatment at 228 nm. The relative standard deviations (R.S.D.) were below 1.6% for the compounds. The correlation coefficient was greater than 0.999 for both compounds in the calibration range. The recoveries at 5 mg/L concentration averaged as 95% for atropine and 102% for obidoxime. The uncertainty of the measurements for atropine and obidoxime was 2.8% and 2.4%, respectively.
authors.affiliation: TUBITAK UME (National Metrology Institute), 41470 Gebze-Kocaeli, Turkey. ahmet.goren@ume.tubitak.gov.tr
authors.forename: Ahmet C
authors.initials: AC
authors.lastname: Gören
authors.forename: Gökhan
authors.initials: G
authors.lastname: Bilsel
authors.forename: Mine
authors.initials: M
authors.lastname: Bilsel
authors.forename: Serpil
authors.initials: S
authors.lastname: Yenisoy-Karaka
authors.forename: Duran
authors.initials: D
authors.lastname: Karaka
mesh_terms: D001285:Atropine / Q000008:administration & dosage / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007274:Injections, Intraperitoneal / Q000295:instrumentation*; D009768:Obidoxime Chloride / Q000008:administration & dosage / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009768:Obidoxime Chloride; D001285:Atropine