High salinity reduces the content of a highly abundant 23-kDa protein of the mangrove Bruguiera parviflora.
A significant decrease in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 23 kDa and pI = 6.5, was observed in leaves of NaCl-treated Bruguiera parviflora (Roxb.) Wt. & Arn. ex Griff. seedlings. This particular salt-sensitive protein, designated as SSP-23, almost disappeared after 45 days of treatment in 400 mM NaCl as compared to untreated seedlings (0 mM NaCl) where the presence of the protein was significant. A polyclonal antibody raised against the 23-kDa protein was used to determine the subcellular localization of this protein in leaves by cross-reaction with proteins from isolated chloroplasts, mitochondria, peroxisomes and cytosol fractions on Western blots. SSP-23 was confirmed to be localized in the cytosol by immunoblotting. The disappearance of SSP-23 as a result of high NaCl treatment suggests that this protein is salt-sensitive and has a possible role in salt adaptation.
Parida
Asish Kumar
AK
National Institute for Plant Biodiversity Conservation and Research, Nayapalli, 751015 Bhubaneswar, Orissa, India.
Mittra
Bhabatosh
B
Das
Anath Bandhu
AB
Das
Taposh Kumar
TK
Mohanty
Prasanna
P
D015153:Blotting, Western; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009994:Osmolar Concentration; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D043982:Rhizophoraceae / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
D010940:Plant Proteins; D012965:Sodium Chloride