Retinal is the essential form of retinoid for storage and transport in the adult of the ascidian Halocynthia roretzi.
Retinoids in the organs (gonad [GND], body wall muscle [BWM], hepatopancreas [HP], gill, hemolymph cells and hemolymph plasma) of the adult ascidian Halocynthia roretzi were analyzed by high performance liquid chromatography. Retinal (RAL) occurred in every organ examined, and most of RAL (>/=99%) was localized in the GND and BWM. None of the organs contained significant amounts of retinol (ROL) or retinyl ester (RE). Lipid droplets, which are characteristic of stellate cells (RE-storing cells of vertebrates), could not be found in the GND, BWM and HP by microscopic observations. These results indicate that this ascidian lacks the RE-storing mechanism, which is ubiquitous in adult vertebrates. The amount and localization of RAL showed the annual change in relation to the reproductive cycle. During summer, the growing season, RAL was present in both GND and BWM at a ratio of about 3:2. From summer to winter, RAL in the GND gradually increased, concomitant with the decrease of RAL in the BWM. In winter, the spawning season, most of RAL was present in the GND (ca. 98%). RAL appears to be accumulated first in the BWM and transported to oocytes accompanying yolk accumulation. ROL and RE were not implicated in the storage and transport of retinoids. The results in the present research strongly suggest that retinoic acid (RA) is produced by the two-step enzymatic reaction: carotenoid cleavage to RAL followed by RAL oxidation to RA and that the prevertebrate chordate lacks ROL-metabolizing systems.
Irie
Toshiaki
T
Osaka Meijo Women's College, Kumatori-cho, Sennan-gun, Osaka 590-0493, Japan. irie@med.akita-u.ac.jp
Kajiwara
Shogo
S
Kojima
Naosuke
N
Senoo
Haruki
H
Seki
Takaharu
T
D000818:Animals; D002338:Carotenoids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004952:Esters / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism / Q000502:physiology; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D010084:Oxidation-Reduction; D012098:Reproduction / Q000502:physiology; D012172:Retinaldehyde / Q000378:metabolism*; D012176:Retinoids / Q000378:metabolism*; D012621:Seasons; D014561:Urochordata / Q000378:metabolism* / Q000502:physiology
D004952:Esters; D012176:Retinoids; D002338:Carotenoids; D012172:Retinaldehyde