Enamel matrix derivative and guided tissue regeneration in the treatment of dehiscence-type defects: a histomorphometric study in dogs.
BACKGROUND
The goal of this investigation was to histologically and histometrically evaluate the healing process of dehiscence-type defects treated by enamel matrix derivative (EMD) and/or guided tissue regeneration (GTR).


METHODS
Seven mongrel dogs were used. Buccal osseous dehiscences were surgically created on the mesial roots of the mandibular third and fourth premolars. The defects were exposed to plaque accumulation for 3 months. After this period, the defects were randomly assigned to one of the treatments: open flap debridement (OFD), enamel matrix derivative (EMD), GTR with bioabsorbable membrane (GTR), and the combination of both procedures (EMD + GTR). After 4 months of healing, the dogs were sacrificed and the blocks were processed. The histometric parameters evaluated included gingival recession, epithelial length, connective tissue adaptation, new cementum, and new bone.


RESULTS
A superior length of new cementum was observed in the sites treated by EMD (3.7 mm) and EMD + GTR (3.8 mm) in comparison with OFD (2.4 mm) (P < 0.05). No statistically significant differences were found in the remaining histometric parameters.


CONCLUSIONS
Within the limits of this study, it can be concluded that EMD alone or in combination with GTR barriers may effectively promote new cementum formation. The combination of both therapies may not provide additional benefits.
Sallum
Enilson A
EA
Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, State University of Campinas, São Paulo, Brazil. easallum@fop.unicamp.br
Pimentel
Suzana P
SP
Saldanha
Juliana B
JB
Nogueira-Filho
Getúlio R
GR
Casati
Marcio Z
MZ
Nociti
Francisco H Júnior
FH
Sallum
Antonio Wilson
AW
D020341:Absorbable Implants; D016301:Alveolar Bone Loss / Q000473:pathology / Q000601:surgery*; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D001641:Bicuspid; D003238:Connective Tissue / Q000473:pathology; D003646:Debridement; D003739:Dental Cementum / Q000473:pathology; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D004285:Dogs; D004848:Epithelium / Q000473:pathology; D005260:Female; D005889:Gingival Recession / Q000473:pathology / Q000601:surgery; D016557:Guided Tissue Regeneration, Periodontal; D007091:Image Processing, Computer-Assisted; D008567:Membranes, Artificial; D011897:Random Allocation; D013524:Surgical Flaps; D014945:Wound Healing / Q000502:physiology
D003746:Dental Enamel Proteins; D008567:Membranes, Artificial; C109069:enamel matrix proteins