[In vitro and ex vivo EPR investigation of metabolic changes in blood under the action of radiotoxins obtained from irradiated potato tubers].
The effect of radiotoxin (RT) obtained from y-irradiated potato tubes on blood of sheep and mice has been investigated by using in vitro and ex vivo EPR. In experiments in vitro, the action of different preparations (RT, extract from unirradiated potato tubers, 1%-HCl or 30%-hydrogen peroxide) on sheep blood has been compared. It has been established that RT is an effective oxidant (like 1%-HCl) of haem iron that leads to an increase of the methemoglobin concentration. The specific peculiarity of RT effect on blood in vitro is an appearance of two well-resolved lines from methemoglobin belonging, probably, to different paramagnetic centers. The signal from nonspecific complexes of Fe3+ has been also observed. Ex vivo EPR spectra markedly differ from these obtained in experiments in vitro. An additional line with g approximately 2.005 and width 6 G in 30 minutes after intraperitoneal RT injection in the lethal dose (0.2 ml of preparation containing of 2 mg RT) has been revealed. Subsequent intoxication of mice is accompanied by the appearance of the signal from nitrosyl complexes in EPR spectra. These differences in experimental results of in vitro and ex vivo EPR can be explained by launch of compensatory adaptive response of organism on the action of highly toxic preparation.
Ibragimova
M I
MI
Petukhov
V Iu
VIu
Zheglov
E P
EP
Koniukhov
G V
GV
Nizamov
R N
RN
D000818:Animals; D001769:Blood; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005720:Gamma Rays; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000633:toxicity*; D011868:Radioisotopes / Q000633:toxicity*; D012756:Sheep; D011198:Solanum tuberosum / Q000528:radiation effects*
D010936:Plant Extracts; D011868:Radioisotopes