[Influence of pyridoxal phosphate in measuring aminotransferases activities in patients with viral hepatitis].
Effect of a pyridoxal phosphate (PP) supplementation of reagents used for ALT and AST measurement was studied in serum of 20 patients suffering from viral hepatitis. Measurements of enzyme activities were carried out at 37 degrees C, using an automate (AU 600, Olympus). Significant differences (p < 0.0001) were observed both for ALT and AST, meanwhile they were more marked for ALT than for AST. This difference was associated with a strong interindividual variability regarding PP activation effect on ALT. In conclusion, aminotransferase measurements should be carried out with a reagent supplemented with PP, when the enzyme activity is used to evaluate a cytolysis. The same recommendation applies when ALT results are integrated into various combinations developed for the evaluation of liver status.
Férard
G
G
Laboratoire de biochimie appliquée de la faculté de pharmacie et Laboratoire du centre de traumatologie et d'orthopédie, Université Louis Pasteur de Strasbourg, Illkirch. georgesferard@evc.net
Imbert-Bismut
F
F
Messous
D
D
Piton
A
A
Abella
A
A
Burnat
P
P
Hainque
B
B
Glasser
N
N
Lessinger
J M
JM
D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood* / Q000187:drug effects*; D001219:Aspartate Aminotransferases / Q000097:blood* / Q000187:drug effects*; D005260:Female; D006525:Hepatitis, Viral, Human / Q000097:blood* / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011732:Pyridoxal Phosphate / Q000494:pharmacology*
D011732:Pyridoxal Phosphate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase