title: Preparative isolation and purification of psoralen and isopsoralen from Psoralea corylifolia by high-speed counter-current chromatography.
abstract: Psoralen and isopsoralen were separated from Psoralea corylifolia by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:5:4.5:5.5, v/v) was used for HSCCC separation, and yielded, from 100 mg of crude extract, 39.6 mg of psoralen and 50.8 mg of isopsoralen each at over 99% purity as determined by high performance liquid chromatography (HPLC). The identification of psoralen and isopsoralen were performed with 1H NMR and 13C NMR.
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. renminliu@lctu.edu.cn
authors.forename: Renmin
authors.initials: R
authors.lastname: Liu
authors.forename: Aifeng
authors.initials: A
authors.lastname: Li
authors.forename: Ailing
authors.initials: A
authors.lastname: Sun
authors.forename: Lingyi
authors.initials: L
authors.lastname: Kong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005363:Ficusin / Q000302:isolation & purification*; D011564:Furocoumarins / Q000302:isolation & purification*; D029907:Psoralea / Q000737:chemistry*
chemical_list: D011564:Furocoumarins; C011659:angelicin; D005363:Ficusin