title: Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes.
abstract: Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.
authors.affiliation: Centre de Recherches du Service de Santé des Armées, 24 Avenue des maquis du Grésivaudan, B.P. 87, F-38702 La Tronche, France. fthibault@crssa.net
authors.forename: F M
authors.initials: FM
authors.lastname: Thibault
authors.forename: E
authors.initials: E
authors.lastname: Valade
authors.forename: D R
authors.initials: DR
authors.lastname: Vidal
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D005896:Glanders / Q000382:microbiology; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D006801:Humans; D006868:Hydrolysis; D008554:Melioidosis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D015342:DNA Probes