title: Stachybotrys chartarum alters surfactant-related phospholipid synthesis and CTP:cholinephosphate cytidylyltransferase activity in isolated fetal rat type II cells.
abstract: Stachybotry chartarum, a fungal contaminant of water-damaged buildings commonly grows on damp cellulose-containing materials. It produces a complex array of mycotoxins. Their mechanisms of action on the pulmonary system are not entirely clear. Previous studies suggest spore products may depress formation of disaturated phosphatidylcholine (DSPC), the major surface-active component of pulmonary surfactant (PS). If S. chartarum can indeed affect formation of this phospholipid, then mold exposure may be a significant issue for pulmonary function in both mature lung and developing fetal lung. To address this possibility, fetal rat type II cells, the principal source of DSPC, were used to assess effects of S. chartarum extract on formation of DSPC. Isolated fetal rat lung type II cells prelabeled with 3H-choline and incubated with spore extract showed decreased incorporation of 3H-choline into DSPC. The activity of CTP:cholinephosphate cytidylyltransferase (CPCT), the rate-limiting enzyme in phosphatidylcholine synthesis was reduced by approximately 50% by a 1:10 dilution of spore extract. Two different S. chartarum extracts (isolates from S. chartarum (Cleveland) and S. chartarum (Hawaiian)) were used to compare activity of CPCT in the presence of phosphatidylglycerol (PG), a known activator. PG produced an approximate two-fold increase in CPCT activity. The spore isolate from Hawaii did not alter enzyme activity. S. chartarum (Cleveland) eliminated the PG-induced activation of CPCT. These results support previous observations that mold products alter PS metabolism and may pose a risk in developing lung, inhibiting surfactant synthesis. Different isolates of the same species of fungus are not equivalent in terms of potential exposure risks.
authors.affiliation: Department of Oral Biology, Faculties of Dentistry & Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E0W2.
authors.forename: C
authors.initials: C
authors.lastname: Hastings
authors.forename: T
authors.initials: T
authors.lastname: Rand
authors.forename: H T
authors.initials: HT
authors.lastname: Bergen
authors.forename: J A
authors.initials: JA
authors.lastname: Thliveris
authors.forename: A R
authors.initials: AR
authors.lastname: Shaw
authors.forename: G A
authors.initials: GA
authors.lastname: Lombaert
authors.forename: H H
authors.initials: HH
authors.lastname: Mantsch
authors.forename: B L
authors.initials: BL
authors.lastname: Giles
authors.forename: S
authors.initials: S
authors.lastname: Dakshinamurti
authors.forename: J E
authors.initials: JE
authors.lastname: Scott
mesh_terms: D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D019916:Choline-Phosphate Cytidylyltransferase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013172:Spores, Fungal / Q000737:chemistry; D013191:Stachybotrys / Q000502:physiology*; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D010713:Phosphatidylcholines; D010743:Phospholipids; D013501:Surface-Active Agents; D003566:Cytidine Diphosphate Choline; D019916:Choline-Phosphate Cytidylyltransferase; D002794:Choline