title: Effect of cyclodextrin on the activity and secondary structure of horseradish peroxidase.
abstract: The activity and secondary structure of horseradish peroxidase (HRP) was studied in aqueous solution containing alpha-, beta- and gamma-cyclodextrin (CD). The results showed that the activity of HRP was enhanced to different extents by the three kinds of CD. A Fourier Transform infrared (FTIR) spectroscopy study indicated that the amount of alpha-helical structure was important for the activity of HRP. This phenomenon is discussed.
authors.affiliation: College of Life and Environment Science, Shanghai Teachers University, Shanghai 200234, P.R. China.
authors.forename: Jian Bo
authors.initials: JB
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.forename: Chun Gu
authors.initials: CG
authors.lastname: Xia
authors.forename: Shu Ben
authors.initials: SB
authors.lastname: Li
mesh_terms: D002942:Circular Dichroism; D003505:Cyclodextrins / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003505:Cyclodextrins; D006735:Horseradish Peroxidase; D010544:Peroxidases