Glutathione S-transferase in the insect Apis mellifera macedonica kinetic characteristics and effect of stress on the expression of GST isoenzymes in the adult worker bee.
The glutathione S-transferase present in the adult worker bee Apis mellifera macedonica was purified and analyzed for its physicochemical and kinetic properties. The enzyme is heterodimeric with subunit molecular masses of 29 and 25 kDa, respectively. Two main isoenzymes with distinct kinetic properties are present, with isoelectric points of 7.40 for the alkaline and 4.58 for the acidic forms, respectively. The two enzymes are induced independently by factors such as insecticide treatments and environmental conditions, including low temperatures or starvation.
Papadopoulos
Athanasios I
AI
Laboratory of Animal Physiology, Department of Zoology, Faculty of Science, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece. thanpap@bio.auth.gr
Polemitou
Irene
I
Laifi
Pshychoula
P
Yiangou
Astero
A
Tananaki
Chrysoula
C
D000818:Animals; D001516:Bees / Q000201:enzymology*; D003080:Cold Temperature; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000494:pharmacology; D007526:Isoelectric Point; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009570:Nitriles; D010261:Paraoxon / Q000031:analogs & derivatives* / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D007306:Insecticides; D007527:Isoenzymes; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D005982:Glutathione Transferase; D010261:Paraoxon; C008480:methylparaoxon