Characterization of the promoter of the mouse c-Jun NH2-terminal/stress-activated protein kinase alpha gene.
We report the isolation of the mouse JNK/SAPKalpha gene, the determination of its exon/intron organization and the characterization of its promoter region. The mouse JNK/SAPKalpha gene spans a region of 36 kbp and contains 13 exons, which represent about 8% of the gene sequence. Major JNK/SAPKalpha splice variants (I and II) are generated by alternative splicing of exons 7 and 8, respectively, whereas minor variants (III and IV) are generated using cryptic sites located inside exon 9. The regulatory elements of the JNK/SAPKalpha gene are located in a 400-bp region placed upstream of the first exon. The gene lacks a TATA element and the initiation of transcription is located inside a 1-kbp CG island. Two regulatory regions located at -98/-69 and -69/-30 were defined by deletion analysis of the promoter.
Callejo
Ainhoa I
AI
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n. 24007 León, Spain.
Casanova
Emilio
E
Calvo
Pedro
P
Galetto
Román
R
Rodríguez-Rey
José C
JC
Chinchetru
Miguel A
MA
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D016385:TATA Box; D014158:Transcription, Genetic
D048031:JNK Mitogen-Activated Protein Kinases