Isoprostanes: markers and mediators of oxidative stress.
Some years ago it was discovered that prostaglandin F2-like compounds are formed in vivo by nonenzymatic free radical-catalyzed peroxidation of arachidonic acid. Because these compounds are a series of isomers that contain the prostane ring of prostaglandins, they were termed F2-isoprostanes. Intermediates in the isoprostane pathway are prostaglandin H2-like compounds that become reduced to form F2-isoprostanes but also undergo rearrangement in vivo to form E2-, D2-, A2-, J2-isoprostanes, isothromboxanes, and highly reactive gamma-ketoaldehydes, termed isoketals. Analogous compounds have also been shown to be formed from free radical mediated oxidation of docosoahexaenoic acid. Because docosahexaenoic acid is highly enriched in neurons, these compounds have been termed neuroprostanes and neuroketals. An important aspect of the discovery of isoprostanes is that measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. Measurement of F4-neuroprostanes has also proved of value in exploring the role of oxidative stress in neurodegenerative diseases. Products of the isoprostane pathway have been found to exert potent biological actions and therefore may participate as physiological mediators of disease.
Montuschi
Paolo
P
Department of Pharmacology, School of Medicine, Catholic University of the Sacred Heart, Rome, Italy.
Barnes
Peter J
PJ
Roberts
L Jackson
LJ
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004194:Disease / Q000209:etiology; D028441:F2-Isoprostanes / Q000032:analysis* / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D051381:Rats; D013812:Therapeutics
D015415:Biomarkers; D028441:F2-Isoprostanes