[Inhibitory effect of quercetin, rutin and puerarin on HDL oxidation induced by Cu2+].
OBJECTIVE
To evaluate the inhibitory effect of quercetin, rutin and puerarin on the HDL oxidation induced by Cu2+ and to investigate their action on the prevention and cure of atherosclerosis.


METHODS
The serum HDL of healthy human was isolated by the one step density gradient ultracentrifugation. The HDL oxidation was induced by Cu2+ in vitro for different time, quercetin and rutin at 5 micromol/L were added ahead, respectively. HDL was oxidized, then identified by A234, REM, TBARS and protein carbonyls content, and was compared with control group. Inhibition of puerarin was weak.


RESULTS
(1) A234, REM, TBARS and protein carbonyls formation increased gradually during HDL oxidation induced by Cu2+ in vitro. (2) During HDL oxidation induced by Cu2+ in vitro and incubation with quercetin and rutin, the kinetic changes of A234, REM, TBARS and protein carbonyls formation showed lag phases of 2 h, 2-4 h, 2-4 h and 4 h respectively, and showed reductions by 24.2%-49.0% (P<0.001) and 9.4%-42.0% (P<0.01); 3.0%-25.0% and 4.0%-20.7% (P<0.05); 7.9%-34.1% and 1.2%-26.8% (P<0.05); 28.3%-50.4% and 18.6%-64.6% (P<0.001) respectively. In the puerarin group, only protein carbonyls formation showed a lag phase of 4 h and a reduction by 14.1%-52.1% (P<0.001) while its A234, REM, TBARS reduced to a small extent.


CONCLUSIONS
The above findings suggested that quercetin and rutin inhibit oxidation of HDL significantly, but puerarin has less antioxidative function.
Meng
Fang
F
Apolipoprotein Research Unit, Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Liu
Rui
R
Bai
Huai
H
Liu
Bing-wen
BW
Liu
Yu
Y
D000975:Antioxidants / Q000494:pharmacology*; D001161:Arteriosclerosis / Q000097:blood; D003300:Copper / Q000494:pharmacology; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D012431:Rutin / Q000494:pharmacology*
D000975:Antioxidants; D007529:Isoflavones; D008075:Lipoproteins, HDL; D012431:Rutin; D003300:Copper; D011794:Quercetin; C033607:puerarin