[EB virus encoded latent membrane protein 1 mediated expression of transcription factor Ets-1 in nasopharyngeal carcinoma cells].
OBJECTIVE
To elucidate the expression of tanscription factor Ets-1 mediated by EB virus encoded latent membrane protein 1 (LMP1) in nasopharyngeal carcinoma (NPC) cells.


METHODS
LMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2) were used. Expression of LMP1 and Ets-1 was observed after induction with Doxycycline (Dox). Expression of Ets-1 mRNA and protein was detected by RT-PCR and Western blot, respectively. The phosphorylation level of Ets-1 protein was examined by co-immunoprecipitation. The DNA binding activity of Ets-1 was detected by electrophoretic-mobility shift assay (EMSA).


RESULTS
After induction with Dox in pTet-on-LMP1 HNE2 cells, to some extent, the expression of Ets-1 mRNA and protein, its phosphorylation level and DNA binding activity were increased with enhancement of LMP1 expression.


CONCLUSIONS
LMP1 induces transcriptional activation and expression of Ets-1 which may contribute to the development of NPC.
Zeng
Liang
L
Department of Pathology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Liu
Yi-Ping
YP
Wang
Hai
H
Tao
Yong-Guang
YG
Zhao
Xiao-Rong
XR
Li
Wei
W
Cao
Ya
Y
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D004318:Doxycycline / Q000494:pharmacology; D004854:Herpesvirus 4, Human; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D010766:Phosphorylation; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014763:Viral Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
C037662:EBV-associated membrane antigen, Epstein-Barr virus; C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D014157:Transcription Factors; D014763:Viral Matrix Proteins; D004318:Doxycycline