Freezing of human immature oocytes using cryoloops with Taxol in the vitrification solution.
OBJECTIVE
In human frozen immature oocytes, there has been little successful delivery. We examined the feasibility of vitrification solution including Taxol, cytoskeltal stabilizer.


METHODS
We set four experimental groups that immature oocytes has cumulus cells or not, or including Taxol or not in the vitrification solution. Frozen-thawed oocytes have been performed IVM, ICSI, and IVC.


RESULTS
There were no significant differences in survival, maturation, and fertilization rate, respectively. However, in the group enveloped by cumulus cells and including Taxol in the vitrification solution, one embryo was developed to blastocyst.


CONCLUSIONS
Our results showed that using vitrification solution with Taxol proved so effective.
Fuchinoue
Kohei
K
Department of Gynecology and Urology, Ladies Clinic Kyono, Japan. lckyono-3@yacht.ocn.ne.jp
Fukunaga
Noritaka
N
Chiba
Setsuyo
S
Nakajo
Yukiko
Y
Yagi
Akiko
A
Kyono
Koichi
K
D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology*; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted
D003451:Cryoprotective Agents; D017239:Paclitaxel