title: [Construction and identification of anti-chymopapain scFv phage display library].
abstract: OBJECTIVE
  To construct phage display library of anti-chymopapain scFv.
  
  
  METHODS
  V(H) and V(L) gene repertoires were amplified from splenocyte mRNA by RT-PCR and joined by a (Gly(4)ser)3 linker to obtain scFv genes. The scFv genes were then cloned into phagemid pFAB5C to construct phage display library. Affinity selection and ELISA were used to identify specific phage antibody to chymopapain.
  
  
  RESULTS
  After 4 rounds of panning, high affinity scFv was obtained.
  
  
  CONCLUSIONS
  Phage display library of anti-chymopapain scFv was successfully constructed, and scFv with binding ability to chymopapain was obtained.
authors.affiliation: Department of Bioengineering, South China University of Technology, Guangzhou 510640, China. bestwjp@sohu.com
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Shu-jin
authors.initials: SJ
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Tai-cheng
authors.initials: TC
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D002917:Chymopapain / Q000276:immunology* / Q000378:metabolism; D005260:Female; D050438:Genes, Immunoglobulin Heavy Chain; D050439:Genes, Immunoglobulin Light Chain; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D011485:Protein Binding
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D002917:Chymopapain