Nuclear CD38 in retinoic acid-induced HL-60 cells.
The cell surface antigen, CD38, is a 45-kDa transmembrane protein which is predominantly expressed on hematopoietic cells during differentiation. As a bifunctional ectoenzyme, it catalyzes the synthesis of cyclic ADP-ribose (cADPR) from NAD(+) and hydrolysis of either NAD(+) or cADPR to ADP-ribose. All-trans-retinoic acid (RA) is a potent and specific inducer of CD38 in myeloid cells. In this report, we demonstrate that the nuclei of RA-treated human HL-60 myeloblastic cells reveal enzymatic activities inherent to CD38. Thus, GDP-ribosyl cyclase and NAD(+) glycohydrolase activities in the nuclear fraction increased very significantly in response to incubation with RA. With Western blotting, we detected in the nuclear protein fraction from RA-treated cells a approximately 43-kDa protein band which was reactive with the CD38-specific monoclonal antibody OKT10. The expression of CD38 in HL-60 nuclei was also shown with FACScan analysis. RA treatment gave rise to an increase in in vitro ADP ribosylation of the approximately 43-kDa nuclear protein. Moreover, nuclei isolated from RA-treated HL-60 cells revealed calcium release in response to cADPR, whereas a similar response was not observed in control nuclei. These results suggest that CD38 is expressed in HL-60 cell nuclei during RA-induced differentiation.
Yalcintepe
Leman
L
Department of Biophysics, Istanbul University, Istanbul Faculty of Medicine, 34390 Capa-Istanbul, Turkey. lemany@istanbul.edu.tr
Albeniz
Isil
I
Adin-Cinar
Suzan
S
Tiryaki
Demir
D
Bermek
Engin
E
Graeff
Richard M
RM
Lee
Hon Cheung
HC
D036541:ADP-ribosyl Cyclase / Q000276:immunology*; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000276:immunology*; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism*; D036563:Cyclic ADP-Ribose / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D008562:Membrane Glycoproteins; D014212:Tretinoin / Q000494:pharmacology*
D015703:Antigens, CD; D008562:Membrane Glycoproteins; D036563:Cyclic ADP-Ribose; D014212:Tretinoin; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1; D002118:Calcium