Comparison of enzymatic properties of DNA-abzymes and human DNAses.
DNA-hydrolyzing antibodies (DNA-abzymes, Abz) were shown to be good biochemical markers of some autoimmune diseases such as systemic lupus erythematosus (SLE) and multiple sclerosis (MS). To better understand mechanisms of abzyme generation, one needs to know optimal conditions for DNA hydrolysis by DNA-abzymes, as well as their enzymatic properties in comparison with those of enzymes possessing the same activity. In contrast to human urine deoxyribonucleases, DNA-hydrolyzing antibodies efficiently digested both single- and double-strand DNA. It was shown that polyclonal antibodies (Abs) in MS may contain up to several types of DNase activities, either activated by metal ions or not.
Baranovskii
A G
AG
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. baranoff@niboch.nsc.ru
Odintsova
E S
ES
Buneva
V N
VN
Doronin
B M
BM
Nevinsky
G A
GA
D017151:Antibodies, Catalytic / Q000378:metabolism*; D001483:Base Sequence; D003851:Deoxyribonucleases / Q000378:metabolism* / Q000652:urine; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006868:Hydrolysis; D008180:Lupus Erythematosus, Systemic / Q000201:enzymology / Q000652:urine; D009103:Multiple Sclerosis / Q000201:enzymology / Q000652:urine; D013379:Substrate Specificity
D017151:Antibodies, Catalytic; D003851:Deoxyribonucleases