The enzymology of cystathionine biosynthesis: strategies for the control of substrate and reaction specificity.
The ability of enzymes to catalyze specific reactions, while excluding others, is central to cellular metabolism. Control of reaction specificity is of particular importance for enzymes that employ catalytically versatile cofactors, of which pyridoxal 5'-phosphate is a prime example. Cystathionine gamma-synthase and cystathionine beta-synthase are the first enzymes in the transsulfuration and reverse transsulfuration pathways, respectively. Each of them occupies branch-point positions in amino acid metabolism and as such are subject to transcriptional and post-translational regulation. Both enzymes catalyze the pyridoxal 5'-phosphate-dependent formation of l-cystathionine; however, their substrate and reaction specificities are distinct. The mechanisms whereby these enzymes control the chemistry of the cofactor are the subject of this review.
Aitken
Susan M
SM
Department of Biology, Carleton University, Ottawa, Ontario, Canada K1S 5B6. susan_aitken@carleton.ca
Kirsch
Jack F
JF
D000494:Allosteric Regulation; D019943:Amino Acid Substitution; D001665:Binding Sites; D019757:Carbon-Oxygen Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002384:Catalysis; D020134:Catalytic Domain; D003540:Cystathionine / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D003541:Cystathionine beta-Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019281:Dimerization; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic; D006710:Homocysteine / Q000378:metabolism; D007700:Kinetics; D010084:Oxidation-Reduction; D017434:Protein Structure, Tertiary; D011732:Pyridoxal Phosphate / Q000378:metabolism; D013237:Stereoisomerism; D013379:Substrate Specificity
D006710:Homocysteine; D003540:Cystathionine; D011732:Pyridoxal Phosphate; C022696:O-succinylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases; D003541:Cystathionine beta-Synthase