Use of multiphoton imaging for studying cell migration in the mouse.
We describe a method for studying cell motility in the living mouse using multiphoton microscopy. The procedure consists of mouse anesthesia, labeling of target cells with enhanced green fluorescent protein by infection with recombinant adenovirus, implantation of beads carrying chemoattractant, preparation of the mouse for imaging, and imaging of individual cell motions via multiphoton microscopy. Two-photon fluorescence excitation of enhanced green fluorescent protein allows visualization of cells within the dermis, whereas second harmonic generation (a non-linear scattering process) allows a simultaneous detailed definition of the dermis structure.
Flesken-Nikitin
Andrea
A
Department of Biomedical Sciences, Cornell University, Ithaca, NY, USA.
Williams
Rebecca M
RM
Zipfel
Warren R
WR
Webb
Watt W
WW
Nikitin
Alexander Yu
AY
D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D018932:Chemokine CCL2 / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D014162:Transfection
D018932:Chemokine CCL2; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins