A systemic iridoviral disease in mullet, Mugil cephalus L., and tiger grouper, Epinephelus fuscoguttatus Forsskal: a first report and study.
A systemic iridoviral disease associated with high mortality was initially recognized in cultured mullet, Mugil cephalus L., and tiger grouper, Epinephelus fuscoguttatus Forsskal, by histopathology and transmission electron microscopy. Polymerase chain reaction was performed on tissues and viral isolates, using four published primer sets developed for the Red Sea bream iridovirus (RSIV). An indirect fluorescent antibody test was also performed on virus-infected ATCC gruntfin (GF) and seabass, Lates calcarifer Bloch, (SB) cells using a monoclonal antibody, RSIV M10. Our results suggested that the mullet and tiger grouper iridovirus bears genetic and antigenic similarities to RSIV.
Gibson-Kueh
S
S
Aquatic Animal Health Branch, AgriFood and Veterinary Authority of Singapore, 6 Perahu Road, Singapore 718827. susan_kueh@ava.gov.sg
Ngoh-Lim
G H
GH
Netto
P
P
Kurita
J
J
Nakajima
K
K
Ng
M L
ML
D000818:Animals; D000911:Antibodies, Monoclonal; D017756:Aquaculture; D004266:DNA Virus Infections / Q000235:genetics / Q000662:veterinary*; D005393:Fish Diseases / Q000821:virology*; D019084:Fluorescent Antibody Technique, Indirect; D007497:Iridoviridae / Q000235:genetics* / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure / Q000821:virology; D046529:Microscopy, Electron, Transmission; D010473:Perciformes; D016133:Polymerase Chain Reaction; D023701:Smegmamorpha
D000911:Antibodies, Monoclonal