The high prevalence of Helicobacter sp. in porcine pyloric mucosa and its histopathological and molecular characteristics.
This study examined the prevalence of Helicobacter infection in the pyloric mucosa of pigs and its histopathological and molecular characteristics. Forty porcine pyloric samples were examined for Helicobacter infection by silver staining and PCR assay. The PCR product (376 bp) was digested with NdeII to differentiate between Helicobacter heilmannii and Helicobacter pylori. Another PCR assay run to produce an 1157 bp fragment was performed using a primer set designed from the 16S rRNA gene of Candidatus H. suis, and its product was cloned and sequenced. Infection rates were 62.5% (25/40) and 95.0% (38/40) as determined by silver staining and the PCR assay, respectively. On histopathological examination, lymphoid follicle aggregation in the pyloric mucosa and granulocytic migration into the lumen of pyloric glands were observed in 24 (60.0%) and 33 (82.5%) gastric samples, respectively. All PCR products, except that of H. pylori, were cut into two fragments of 147 and 229 bp by enzymatic digestion with NdeII. Sequencing of the 16S rRNA gene showed that the bacterium had 99.57% (1152 bp/1157 bp) homology to the 16S rRNA gene of Candidatus H. suis.
Park
Jong-Hwan
JH
Department of Laboratory Animal Medicine, School of Agricultural Biotechnology, College of Veterinary Medicine, Seoul National University, San 56-1, Sillim-Dong, Kwanak-Gu, Seoul 151-742, South Korea.
Seok
Seung-Hyeok
SH
Cho
Sun-A
SA
Baek
Min-Won
MW
Lee
Hui-Young
HY
Kim
Dong-Jae
DJ
Park
Jae-Hak
JH
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D005784:Gene Amplification; D016998:Helicobacter / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D011706:Pyloric Antrum / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S