Effect of single wall carbon nanotubes on human HEK293 cells.
The influence of single-walled carbon nanotubes (SWCNTs) on human HEK293 cells is investigated with the aim of exploring SWCNTs biocompatibility. Results showed that SWCNTs can inhibit HEK293 cell proliferation, decrease cell adhesive ability in a dose- and time-dependent manner. HEK293 cells exhibit active responses to SWCNTs such as secretion of some 20-30 kd proteins to wrap SWCNTs, aggregation of cells attached by SWCNTs and formation of nodular structures. Cell cycle analysis showed that 25 microg/ml SWCNTs in medium induced G1 arrest and cell apoptosis in HEK293 cells. Biochip analysis showed that SWCNTs can induce up-regulation expression of cell cycle-associated genes such as p16, bax, p57, hrk, cdc42 and cdc37, down-regulation expression of cell cycle genes such as cdk2, cdk4, cdk6 and cyclin D3, and down-regulation expression of signal transduction-associated genes such as mad2, jak1, ttk, pcdha9 and erk. Western blot analysis showed that SWCNTs can induce down-regulation expression of adhesion-associated proteins such as laminin, fibronectin, cadherin, FAK and collagen IV. These results suggest that down-regulation of G1-associated cdks and cyclins and upregulation of apoptosis-associated genes may contribute to SWCNTs induced G1 phase arrest and cell apoptosis. In conclusion, SWCNTs can inhibit HEK293 cells growth by inducing cell apoptosis and decreasing cellular adhesion ability.
Cui
Daxiang
D
Max Planck Institute for Metals Research, Heisenbergstrasse 3, D70569 Stuttgart, Germany. daxiangcui@yahoo.com
Tian
Furong
F
Ozkan
Cengiz S
CS
Wang
Mao
M
Gao
Huajian
H
D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry; D015153:Blotting, Western; D002244:Carbon / Q000633:toxicity*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056743:Cyclin D3; D016213:Cyclins / Q000096:biosynthesis / Q000235:genetics; D053938:DNA Fragmentation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000032:analysis / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; C534696:CCND3 protein, human; D015815:Cell Adhesion Molecules; D056743:Cyclin D3; D016213:Cyclins; D012313:RNA; D002244:Carbon