[A study on expression of hsp70 mRNA in the early stage of diffuse brain injury in rats].
OBJECTIVE
To evaluate the changes of hsp70 mRNA in neuron and glia of rat suffering early diffuse brain injury.


METHODS
SD rats were used in the replication of the animal pattern of diffuse brain injury. In situ hybridization technique and image analysis technique were applied in detecting the hsp70 mRNA in the rat's cerebral cortex, thalamus and brain stem.


RESULTS
The signal intensity of hsp70 mRNA could be detected in 5 minutes after brain injury; subsequently, it attained to the summit at 6 hours and declined at 12 hours after brain injury.


CONCLUSIONS
The above data suggest that hsp70 mRNA is useful for the diagnosis of early diffuse brain injury and for distinguishing the postmortem injury from antemortem injury.
Wang
Ye
Y
Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Pan
Hong-fu
HF
Zhang
Kui-ming
KM
Chen
Xiao-gang
XG
Peng
Qi-yi
QY
Wang
Wen-dong
WD
Liu
Min
M
D000818:Animals; D015415:Biomarkers; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger