Transcriptional profiling of the early stages of germination in Candida albicans by real-time RT-PCR.
By using real-time RT-PCR, we profiled the expression of CGR1, CaMSI3, EFG1, NRG1, and TUP1 in Candida albicans strains JCM9061 and CAI4 under several conditions, including induction of morphological transition, heat shock, and treatment with calcium inhibitors. Expression of CaMSI3 changed under these growth conditions except during heat shock. CGR1 expression increased during the early stages of hyphal growth in JCM9061, while expression was strain-dependent during heat shock. Both EFG1 and NRG1 were similarly expressed under hypha-inducing conditions and heat shock. Expression of TUP1 was slightly different from the expression of EFG1 or NRG1.
Toyoda
Mika
M
Section of Infection Biology, Department of Functional Bioscience, Fukuoka Dental College, Fukuoka 814-0193, Japan.
Cho
Tamaki
T
Kaminishi
Hidenori
H
Sudoh
Masayuki
M
Chibana
Hiroji
H
D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D019319:Okadaic Acid / Q000494:pharmacology; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019284:Thapsigargin / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*
C426046:CGR1 protein, Candida albicans; D004268:DNA-Binding Proteins; C121438:EFG1 protein, Candida albicans; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D012331:RNA, Fungal; D012097:Repressor Proteins; D014157:Transcription Factors; D019319:Okadaic Acid; D000001:Calcimycin; D019284:Thapsigargin; D002118:Calcium