title: [The T cells activated by dendritic cells derived from AML cells: a study of their cytotoxic effect on autologous AML cells].
abstract: OBJECTIVE
  To culture dendritic cells (DCs) from human acute myeloid leukemia (AML) cells, and to observe the cytotoxic effect of the T lymphocytes that are activated by DCs pulsed with leukemic antigen peptides (LAP) or LAP-heat shock protein 90 (HSP90) on autologous AML cells.
  
  
  METHODS
  Mononuclear cells (MNC) were isolated from peripheral blood (PB) and bone marrow (BM) of patients with AML by density gradient centrifugation; DCs were induced from MNC of PB in a special culture system including recombinant human granulocyte-monocyte colony stimulating factor (rhGM-CSF), interleukin-4 (rhIL-4) and tumor necrosis factor-alpha (TNF-alpha) for 7-14 days. These DCs were identified by morphology and immunophenotype studies; LAP were obtained by repeatedly freezing and thawing AML cells respectively. DCs were pulsed with LAP and LAP-HSP90 compounds respectively. The cytotoxity of T lymphocytes activated by antigen pulsed DCs on autologous AML cells was measured in vitro by the lactate dehydrogenase-release assay.
  
  
  RESULTS
  After 7-14 days' culture, (6-11) x 10(4) DCs were obtained from 1 x 10(6) MNC of PB in 3 AML cases. The cytotoxity rates of T lymphocytes, from 3 AML cases, activated by DCs pulsed with LAP and LAP-HSP90 compounds were (35.33+/-9.04)% and (62.07+/-8.29)% respectively.
  
  
  CONCLUSIONS
  Effective cytotoxity of T lymphocytes to the autologous leukemia cells can be induced by DCs pulsed with LAP and LAP-HSP90 compounds respectively, but the effectiveness induced by the latter is more powerful than that induced by the former.
authors.affiliation: Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xiang
authors.forename: Sheng-fu
authors.initials: SF
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Yong-qiu
authors.initials: YQ
authors.lastname: Mao
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Yang
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm