Association of human leukocyte antigen DQ1 and dengue fever in a white Southern Brazilian population.
Dengue is an infectious disease of viral etiology transmitted by the mosquitoes Aedes aegypti, A. albopictus, and A. scutellaris. It can develop either as a benign form or as a severe hemorrhagic form. Previous work showed an association of the hemorrhagic form with human leukocyte antigens (HLA), suggesting a role of genetic factors in disease susceptibility. Nevertheless, data on HLA association with the classical form of the disease is scarce in literature. Sixty-four patients and 667 normal individuals, living in the state of Parana, Southern Brazil, were used as test and control group, respectively. The patients developed the disease during a virus 1 dengue outbreak either in Maringa city in 1995 (47) or in Paranavai city in 1999 (17). The diagnostic was confirmed through serology and/or viral culture. HLA class I and II typing was performed by the classical microlynfocitotoxicity test using monoclonal antisera and fluorobeads. Qui-square statistical analysis confirmed a positive association with HLA-DQ1 (76.6% vs 57.7%; p = 0.005243; pc = 0.026215). HLA-DR1 also presented an increased frequency in the test group, not statistically significant after p correction though (32.8% vs 15.9%; p = 0.005729; pc = 0.080206). In conclusion, genetic factors may play a role on the susceptibility to the classical dengue, virus 1, in the Brazilian population. Further independent studies should be performed in the Brazilian population to confirm these preliminary data.
Polizel
José Roberto
JR
Departamento de Análises Clínicas, Universidade Estadual de Maringá, Av. Colombo 5790, 87020-900 Maringá, PR, Brazil.
Bueno
Danilo
D
Visentainer
Jeane Eliete L
JE
Sell
Ana Maria
AM
Borelli
Sueli Donizete
SD
Tsuneto
Luiza Tamie
LT
Dalalio
Marcia Machado O
MM
Coimbra
Maria Teresa M
MT
Moliterno
Ricardo Alberto
RA
D001938:Brazil; D016022:Case-Control Studies; D003715:Dengue / Q000235:genetics* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D012307:Risk Factors
D006683:HLA-DQ Antigens