title: [Genetic association between interleukins gene polymorphisms with primary biliary cirrhosis in Chinese population].
abstract: OBJECTIVE
  To determine the relationship between polymorphisms in the genes encoding IL-1, IL-6, and IL-10 with primary biliary cirrhosis (PBC) in Chinese population.
  
  
  METHODS
  Whole-blood samples were taken from 77 patients with PBC and 160 healthy controls. DNA was extracted and the polymorphisms at positions IL-1 +3953, IL-1RN intron 2, IL-6 -174, and IL-10 -1082, -819, and -592 were determined by using sequence-specific polymerase chain reaction (SSP) or polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  The frequency of IL-1RN1,1 allele in PBC group was significantly higher than in control group (90.9% vs 79.4%, P=0.026), and the frequency of IL-1RN1,2 in PBC group was significantly lower than in control group (6.5% vs 18.8%, P=0.013). There was no significant difference in the frequence of IL-1RN*2 allele between PBC group and control group (P=0.06). Of the 77 patients with PBC, 4 patients were IL-6 -174GC, 73 were IL-6 174GG. All the 160 health controls are IL-6 -174GG (P=0.0036). The frequence of IL-6 -174C allele in PBC group was significantly higher than that in control group (P=0.0038). No significant differences of polymorphisms for IL-1 +3953 and IL-10 (-1082, -819 and -592) were found between PBC group and control group.
  
  
  CONCLUSIONS
  The polymorphisms of IL-1RN and IL-6 -174G/C appear to be associated with PBC, and the polymorphisms of IL-1 +3953 and IL-10 promoter gene are not associated with PBC in a Chinese population.
authors.affiliation: Department of Experimental Diagnosis, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. flieying@hotmail.com
authors.forename: Lie-ying
authors.initials: LY
authors.lastname: Fan
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ren-qian
authors.initials: RQ
authors.lastname: Zhong
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Tu
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Ye
authors.forename: Qu-bo
authors.initials: QB
authors.lastname: Chen
authors.forename: Wan-jie
authors.initials: WJ
authors.lastname: Zeng
authors.forename: Xian-tao
authors.initials: XT
authors.lastname: Kong
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics*; D008105:Liver Cirrhosis, Biliary / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D016753:Interleukin-10