title: Upregulation and atypical expression of the CD1 molecules on monocytes in sickle cell disease.
abstract: Human CD1 group I molecules CD1a, b, and c are expressed on antigen-presenting cells, notably dendritic cells, and implicated in glycolipids presentation to T lymphocytes. Expression of CD1 on monocytes is a hallmark of their activation. Because monocyte activation has been reported during steady state disease in sickle cell anemia (SCA) patients, we have analyzed CD1 expression on monocytes from 45 SCA patients originating from Africa and 27 healthy control subjects. CD1 expression was detected on monocytes in the majority of SCA patients (75%), whereas it was not observed in the vast majority of the control group (70.4%). CD1b and CD1c were highly expressed in Sbeta thalassemia patients and CD1a expression was predominant in SDPunjab patients. This expression of the CD1 molecules is correlated with an increased expression of the major histocompatibility complex class II invariant chain (CD74). Finally, we have observed that the majority of SCA patients (68%) express only two or one CD1 isoforms. This study demonstrates the particular phenotype of SCA monocytes intermediate between normal resting and activated monocytes, a phenotype that could have consequences on regulation of the infection outcome.
authors.affiliation: INSERM U396, Institut d'Hématologie, Hôpital Saint-Louis, Paris, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Sloma
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Zilber
authors.forename: Dominique
authors.initials: D
authors.lastname: Charron
authors.forename: Robert
authors.initials: R
authors.lastname: Girot
authors.forename: Ryad
authors.initials: R
authors.lastname: Tamouza
authors.forename: Catherine
authors.initials: C
authors.lastname: Gelin
mesh_terms: D000349:Africa / Q000208:ethnology; D000755:Anemia, Sickle Cell / Q000276:immunology / Q000378:metabolism*; D018949:Antigens, CD1 / Q000378:metabolism*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005602:France; D006023:Glycoproteins / Q000378:metabolism; D006444:Hemoglobin C / Q000378:metabolism; D006451:Hemoglobin, Sickle / Q000378:metabolism; D006455:Hemoglobins, Abnormal / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D008297:Male; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation / Q000276:immunology; D017086:beta-Thalassemia / Q000276:immunology / Q000378:metabolism
chemical_list: D018949:Antigens, CD1; D000944:Antigens, Differentiation, B-Lymphocyte; D039481:CD11b Antigen; C437944:CD1C protein, human; C419393:CD1a antigen; C419394:CD1b antigen; D006023:Glycoproteins; D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D000949:Histocompatibility Antigens Class II; C045084:invariant chain; D006444:Hemoglobin C; C026237:hemoglobin D Punjab