Differentiation status dependent function of FOG-1.
The molecular interactions between transcription factors and cofactors play crucial roles in various biological processes, including haematopoiesis. FOG-1 is a cognate cofactor of GATA-1, and the FOG-1/GATA-1 complex is essential for the haematopoietic differentiation of erythroid cells and megakaryocytes. In order to elucidate the biological functions of FOG-1 in the different contexts of cell differentiation, we analysed the effects of FOG-1 expression on haematopoietic cell differentiation, using a combination of in vitro differentiation of mouse embryonic stem (ES) cells and conditional gene expression. FOG-1 suppressed the proliferation of primitive and definitive erythroid cells in all stages of differentiation. However, FOG-1 inhibited and enhanced megakaryopoiesis in the early and late differentiation stages, respectively, through different molecular mechanisms. In addition, FOG-1 inhibited the proliferation of ES cells, the molecular mechanism of which differs from those of erythroid and megakaryocytic cells. These results suggest that FOG-1 functions in a cell differentiation context-dependent manner.
Tanaka
Makoto
M
Department of Pathology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Zheng
Jie
J
Kitajima
Kenji
K
Kita
Keisuke
K
Yoshikawa
Hideki
H
Nakano
Toru
T
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D002454:Cell Differentiation; D015672:Erythroid Precursor Cells / Q000166:cytology*; D015870:Gene Expression; D006410:Hematopoiesis; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D014157:Transcription Factors
D002352:Carrier Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C107310:Zfpm1 protein, mouse