Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402 involving G2-phase arrest.
OBJECTIVE
To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402).


METHODS
Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis.


RESULTS
PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 mumol/L for 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h.


CONCLUSIONS
Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest.
Chen
Hong-ying
HY
College of Life Sciences, Wuhan University, Wuhan 430072, China.
Zheng
Cong-yi
CY
Zou
Guo-lin
GL
Xie
Da-xing
DX
Gong
Jian-ping
JP
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D019925:Cyclin A / Q000378:metabolism; D019926:Cyclin B / Q000378:metabolism; D016193:G1 Phase; D016195:G2 Phase; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D017663:Peplomycin / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D019925:Cyclin A; D019926:Cyclin B; D017663:Peplomycin