Chlorine inhalation produces nasal airflow limitation in allergic rhinitic subjects without evidence of neuropeptide release.
BACKGROUND
Seasonal allergic rhinitic (SAR) subjects are more physiologically reactive to airborne irritants than non-rhinitic (NR) subjects; however the mechanism underlying this difference is unclear.


OBJECTIVE
We sought to determine whether irritant-induced nasal airflow limitation involves neuropeptide release into nasal lining fluid, and if so, whether such release occurs differentially by rhinitic status.


METHODS
Eight SAR and 8 NR subjects were exposed to 1.0 ppm chlorine and filtered air in random order during separate visits; exposures were via nasal mask and lasted 15 min. Rhinomanometry was performed before, immediately post-, and 15 min post-exposure. Following a minimum of 2 weeks' time, exposures and symptom reporting were repeated with nasal lavage pre- and post-exposure. Neuropeptides (substance P, cacitonin gene-related protein, vasoactive intestinal peptide, and neuropeptide Y) as well as markers of plasma leakage (albumin and urea) and glandular secretion (lysozyme and 7F10-mucin) were measured using standard methods.


RESULTS
Cl(2) provocation significantly increased nasal airway resistance in SAR but not NR subjects (p<0.05). Neuropeptide levels in nasal lavage fluid, on the other hand, were unaffected, with the exception of a paradoxical increase in vasoactive intestinal peptide in non-rhinitic controls post-Cl(2) provocation.


CONCLUSIONS
Irritant-induced nasal airflow limitation is more pronounced among SAR than NR subjects. We could not, however, demonstrate a role for neuropeptide release in the nasal congestive response of SAR subjects.
Shusterman
Dennis
D
Upper Airway Biology Laboratory, University of California, San Francisco, Box 0843, San Francisco, CA 94143, USA. dennis3@u.washington.edu
Balmes
John
J
Murphy
Mary Alice
MA
Tai
Chih-Feng
CF
Baraniuk
James
J
D000280:Administration, Inhalation; D000328:Adult; D000403:Airway Resistance; D015415:Biomarkers; D002713:Chlorine / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007509:Irritants / Q000008:administration & dosage*; D008297:Male; D008875:Middle Aged; D017817:Nasal Lavage Fluid; D009479:Neuropeptides / Q000378:metabolism*; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism* / Q000503:physiopathology; D025363:Rhinomanometry
D015415:Biomarkers; D007509:Irritants; D009479:Neuropeptides; D002713:Chlorine