title: Integrated lectin affinity microfluidic chip for glycoform separation.
abstract: Lectin affinity chromatography was miniaturized into a microfluidic format, which results in improvement of performance, as compared to the conventional method. A lectin affinity monolith column was prepared in the microchannel of a microfluidic chip. The porous monolith was fabricated by UV-initiated polymerization of ethylene dimethacrylate (EDMA) and glycidyl methacrylate (GMA) in the presence of porogeneities, followed by immobilization of pisum sativum agglutinin (PSA) on the monolith matrix. Using electroosmosis as the driven force, lectin affinity chromatographies of three kinds of glycoprotein, turkey ovalbumin (TO), chicken ovalbumin (CO), and ovomucoid (OM), were carried out on the microfluidic system. All the glycoproteins were successfully separated into several fractions with different affinities toward the immobilized PSA. The integrated system reduces the time required for the lectin affinity chromatography reaction to approximately 3%, thus, the overall analysis time from 4 h to 400 s. Only 300 pg of glycoprotein is required for the whole separation process. Moreover, troublesome operations for lectin affinity chromatography are simplified.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Mao
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Dai
authors.forename: Keyi
authors.initials: K
authors.lastname: Wang
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D000327:Adsorption; D000818:Animals; D002021:Buffers; D002645:Chickens; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D004563:Electrochemistry; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D009995:Osmosis; D010047:Ovalbumin / Q000737:chemistry; D010059:Ovomucin / Q000737:chemistry; D010316:Particle Size; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014421:Turkey
chemical_list: D002021:Buffers; D006023:Glycoproteins; D037102:Lectins; D012709:Serum Albumin; D010059:Ovomucin; D010047:Ovalbumin