Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis.
Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and gamma-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitative analysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and gamma-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum.
Goicoechea
Hector
H
Department of Chemistry, University of Central Florida, P.O. Box 25000, Orlando, FL 32816-23366, USA.
Roy
Bidhan C
BC
Santos
Marina
M
Campiglia
Andres D
AD
Mallik
Sanku
S
D002256:Carbonic Anhydrases / Q000097:blood*; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D005063:Europium / Q000737:chemistry*; D005069:Evaluation Studies as Topic; D006801:Humans; D016018:Least-Squares Analysis; D008081:Liposomes; D009942:Organometallic Compounds / Q000138:chemical synthesis*; D012709:Serum Albumin / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry*; D005719:gamma-Globulins / Q000032:analysis*
C500711:4-aminiosalicylic acid ethylenediaminetetraacetate terbium(III); C500710:5-aminosalicylic acid ethylenediaminetetraacetate europium(III); D008081:Liposomes; D009942:Organometallic Compounds; D012709:Serum Albumin; D005719:gamma-Globulins; D013725:Terbium; D005063:Europium; D004492:Edetic Acid; D002256:Carbonic Anhydrases