A simple and economic preservation method for genomic bacterial DNA from clinically significant pathogens.
Bacterial culture was allowed to dry to completeness on Columbia agar base with defibrinated horse blood. Following 6 months storage at room temperature, microbial DNA was extracted and successfully amplified by PCR. This storage technique has the advantage over other methods of not requiring (i) a DNA extraction protocol prior to storage and (ii) refrigeration and/or freezing. This technique maybe useful in the transportation of bacterial genomic DNA in nonviable cells as well as reliable method for the storage of DNA in underdeveloped countries.
Moore
John E
JE
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, Northern Ireland, United Kingdom. jemoore@niphl.dnet.co.uk
Xu
Jiru
J
Millar
B Cherie
BC
D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D016972:Enterobacter cloacae / Q000235:genetics*; D016984:Enterococcus faecium / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011309:Preservation, Biological / Q000379:methods* / Q000592:standards; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial