Crystallization and preliminary X-ray crystallographic analysis of a non-specific lipid-transfer protein with antipathogenic activity from Phaseolus mungo.
A 9 kDa non-specific lipid-transfer protein (nsLTP) from mung bean (Phaseolus mungo) seeds, displaying antifungal activity, antibacterial activity and lipid-transfer activity, was crystallized at 297 K using ammonium sulfate as a precipitant by means of the hanging-drop vapour-diffusion method. Native X-ray diffraction data were collected to a resolution of 2.4 A. The crystals are rhombohedral, belonging to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.671, b = 51.785, c = 55.925 A. Assuming the presence of one molecule in the crystallographic asymmetric unit results in a Matthews coefficient (V(M)) of approximately 3.0 A(3) Da(-1), corresponding to a solvent content of about 58%.
Wang
Shao Yun
SY
Institute of Biotechnology, Fuzhou University, Fujian 350002, People's Republic of China.
Zhou
Kang Jing
KJ
Ye
Xiu Yun
XY
Xu
Zhi Bin
ZB
Wu
Jin Hong
JH
Rao
Ping Fan
PF
D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013045:Species Specificity; D013696:Temperature; D014961:X-Ray Diffraction
D000890:Anti-Infective Agents; D049294:Phospholipid Transfer Proteins; D010940:Plant Proteins; D000645:Ammonium Sulfate