Behavioural characterisation of young adult transgenic mice overexpressing galanin under the PDGF-B promoter.
The behavioural phenotype of transgenic mice (3- to 5-months old) overexpressing galanin (GalOE) under the platelet-derived growth factor B (PDGF-B) promoter was evaluated in a battery of tests, including open field, locomotor cages, light-dark exploration test, elevated plus-maze and the Porsolt forced swim test. Learning and memory were assessed in the passive avoidance and the Morris water maze tasks. No difference between genotypes was found in exploratory activity in the open field. GalOE mice showed a slight increase in spontaneous locomotor activity assessed in the locomotor cages, but the amphetamine-induced increase in locomotor activity was somewhat lower in GalOE mice. Anxiety-like behaviour in the three different tests including open field, light-dark exploration and elevated plus-maze did not differ between genotypes. In the Porsolt forced swim test, GalOE mice displayed an increased time of immobility, indicative of increased learned helplessness possibly reflecting increased stress-susceptibility and/or depression-like behaviour. GalOE mice showed normal learning and memory retention in the passive avoidance and the Morris water maze tasks. These data support the hypothesis that galanin may have a role in functions related to mood states including affective disorders.
Kuteeva
Eugenia
E
Department of Neuroscience, Karolinska Institutet, Retzius väg 8, Stockholm S-171 77, Sweden.
Hökfelt
Tomas
T
Ogren
Sven Ove
SO
D000339:Affect; D000662:Amphetamines / Q000494:pharmacology; D000818:Animals; D001007:Anxiety; D019004:Galanin / Q000096:biosynthesis* / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D007858:Learning; D008124:Locomotion; D008297:Male; D018782:Maze Learning; D008568:Memory; D051379:Mice; D008822:Mice, Transgenic; D009043:Motor Activity; D010641:Phenotype; D011401:Promoter Regions, Genetic; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics*; D044088:Receptors, Galanin / Q000378:metabolism; D013315:Stress, Psychological; D013997:Time Factors
D000662:Amphetamines; D020574:Proto-Oncogene Proteins c-sis; D044088:Receptors, Galanin; D019004:Galanin