Effects of a number of classes of 50S inhibitors on stop codon readthrough during protein synthesis.
The effect of a number of antibiotics on stop codon readthrough during protein synthesis in Escherichia coli was examined. Inhibitors which bind close to the entrance of the peptide exit tunnel on the 50S ribosomal subunit promote substantial levels of readthrough, presumably by disrupting the mechanism of peptide release.
Thompson
Jill
J
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA. Jill_Rosemary_Thompson@brown.edu
Pratt
Catherine A
CA
Dahlberg
Albert E
AE
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018388:Codon, Terminator / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018388:Codon, Terminator; D001616:beta-Galactosidase