Integrated selective enrichment target--a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases.
The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples.
Ekström
Simon
S
Department of Electrical Measurements, Lund Institute of Technology, Lund, Sweden. simon.ekstrom@elmat.lth.se
Wallman
Lars
L
Malm
Johan
J
Becker
Charlotte
C
Lilja
Hans
H
Laurell
Thomas
T
Marko-Varga
György
G
D015415:Biomarkers / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D017430:Prostate-Specific Antigen / Q000032:analysis; D011469:Prostatic Diseases / Q000175:diagnosis*; D011471:Prostatic Neoplasms / Q000175:diagnosis; D011506:Proteins / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
D015415:Biomarkers; D002352:Carrier Proteins; D006023:Glycoproteins; D026901:Membrane Transport Proteins; C488892:PIP protein, human; D011506:Proteins; D017430:Prostate-Specific Antigen