title: Regulation of reactive oxygen species (ROS) production by C18 fatty acids in Jurkat and Raji cells.
abstract: In the present study, the effects of C18 fatty acids with different numbers of double bonds, SA (stearic acid; C18:0), OA (oleic acid; C18:1), LA (linoleic acid; C18:2) and gamma-LNA (gamma-linolenic acid; C18:3), on ROS (reactive oxygen species) production by Jurkat (a human T-lymphocyte-derived cell line) and Raji (a human B-lymphocyte-derived cell line) cells were investigated. ROS production was determined by NBT (Nitro Blue Tetrazolium) reduction (intracellular and extracellular ROS production) and by dihydroethidium oxidation using flow cytometry (intracellular ROS production). The effectiveness on ROS production was gamma-LNA<SA<OA<LA in Jurkat cells and SA<gamma-LNA<OA<LA in Raji cells. LA (found in corn, soya bean and sunflower oils) was more potent than OA (found in olive oil) in stimulating ROS production in both Raji and Jurkat cells. The lower ROS production by OA compared with LA may be one of the benefits of olive oil consumption. As SA and gamma-LNA acids had little or no effect, further studies on the site of ROS production in these cells were carried out with OA and LA only. Activation of NADPH oxidase via PKC (protein kinase C) was found to be the major mechanism of ROS production induced by OA and LA in Jurkat and Raji cells.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Av. Prof Lineu Prestes, 1524, CEP 05508-900, SP, Brazil. mafecury@icb.usp.br
authors.forename: Maria F
authors.initials: MF
authors.lastname: Cury-Boaventura
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
mesh_terms: D000704:Analysis of Variance; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D005227:Fatty Acids / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells; D019787:Linoleic Acid / Q000494:pharmacology; D019301:Oleic Acid / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013229:Stearic Acids / Q000494:pharmacology; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D017965:gamma-Linolenic Acid / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D017382:Reactive Oxygen Species; D013229:Stearic Acids; D019301:Oleic Acid; C031183:stearic acid; D017965:gamma-Linolenic Acid; D019787:Linoleic Acid