Identification and analysis of cis-regulatory elements in development using comparative genomics with the pufferfish, Fugu rubripes.
The control of vertebrate development is facilitated by cis-regulatory sequences hardwired into the genome. Given that many developmental processes are strikingly similar across all backboned animals, it is reasonable to expect these sequences to be conserved at the nucleotide level, their potential for mutation being constrained by their function. Comparison between the genomes of highly divergent organisms allows such sequences to be identified and some of the most successful approaches have compared regions from the pufferfish, Fugu rubripes, with its distant mammalian relatives, rodents and humans. This review describes progress made in this kind of comparison, from small regions of individual genes, to whole genome alignments.
Elgar
Greg
G
MRC Rosalind Franklin Centre for Genomics Research, Genome Campus, Hinxton, Cambridgeshire CB10 1SB, UK. gelgar@rfcgr.mrc.ac.uk
D000818:Animals; D019295:Computational Biology / Q000379:methods*; D005136:Eye Proteins / Q000235:genetics; D005809:Genes, Regulator; D016678:Genome; D023281:Genomics / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D005810:Multigene Family; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D012984:Software; D023721:Tetraodontiformes / Q000235:genetics*; D051153:Wnt Proteins
D005136:Eye Proteins; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D051153:Wnt Proteins