Expression of serotonin2A receptors in Purkinje cells of the developing rat cerebellum.
Previous physiological and pharmacological studies have shown that the serotonin2A (5-HT2A) receptor is involved in cerebellar functions. However, the expression of 5-HT2A receptors in the developing cerebellum has not been elucidated to date. In the present immunohistochemical study, we examined developmental changes of the distribution of 5-HT2A receptors in Purkinje cells of the rat cerebellum from embryonic day 18 (E18) to postnatal day 21 (P21). The weak immunoreaction to 5-HT2A receptors was found in the deep cerebellar nuclei on E19. In the cerebellar cortex of the hemisphere and the posterior vermis, somata of Purkinje cells became weakly immunoreactive on P0. With the dendritic elongation and arborization, the immunoreaction appeared in the proximal parts of Purkinje cell dendrites. Distal parts of the dendrites became immunoreactive after P12, and were strongly immunolabeled by P21. The present study may provide a structural basis to investigate the roles of 5-HT2A receptors during the cerebellar development.
Maeshima
Toru
T
Department of Physical Therapy, Tsukuba College of Technology, Tsukuba, Ibaraki 305-0821, Japan. maeshima@k.tsukuba-tech.ac.jp
Shiga
Takashi
T
Ito
Ryuzo
R
Okado
Nobuo
N
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D064026:Calbindins; D002454:Cell Differentiation / Q000502:physiology*; D002525:Cerebellar Cortex / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D002529:Cerebellar Nuclei / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D007120:Immunochemistry; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology
D064026:Calbindins; D044402:Receptor, Serotonin, 5-HT2A; D064030:S100 Calcium Binding Protein G; D012701:Serotonin