The Ste20-like kinase misshapen functions together with Bicaudal-D and dynein in driving nuclear migration in the developing drosophila eye.
Nuclear translocation, driven by the motility apparatus consisting of the cytoplasmic dynein motor and microtubules, is essential for cell migration during embryonic development. Bicaudal-D (Bic-D), an evolutionarily conserved dynein-interacting protein, is required for developmental control of nuclear migration in Drosophila. Nothing is known about the signaling events that coordinate the function of Bic-D and dynein during development. Here, we show that Misshapen (Msn), the fly homolog of the vertebrate Nck-interacting kinase is a component of a novel signaling pathway that regulates photoreceptor (R-cell) nuclear migration in the developing Drosophila compound eye. Msn, like Bic-D, is required for the apical migration of differentiating R-cell precursor nuclei. msn displays strong genetic interaction with Bic-D. Biochemical studies demonstrate that Msn increases the phosphorylation of Bic-D, which appears to be necessary for the apical accumulation of both Bic-D and dynein in developing R-cell precursor cells. We propose that Msn functions together with Bic-D to regulate the apical localization of dynein in generating directed nuclear migration within differentiating R-cell precursor cells.
Houalla
Tarek
T
McGill Centre for Research in Neuroscience, and Department of Neurology and Neurosurgery, McGill University Health Centre, 1650 Cedar Avenue, Montreal, Que., Canada.
Hien Vuong
Dac
D
Ruan
Wenjing
W
Suter
Beat
B
Rao
Yong
Y
D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D002467:Cell Nucleus; D004330:Drosophila / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004398:Dyneins / Q000235:genetics* / Q000378:metabolism; D005123:Eye / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
C061998:BicD protein, Drosophila; D029721:Drosophila Proteins; C113875:msn protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D004398:Dyneins