Characterisation, asymmetry and reproducibility of on- and off-transient pulmonary oxygen uptake kinetics in endurance-trained runners.
The purpose of this study was three-fold: (1) to characterise both the on- and off-transient oxygen uptake (V(.)O(2)) kinetics in endurance runners during moderate-intensity treadmill running; (2) to determine the degree of symmetry between on- and off-transients; and (3) to determine the reproducibility of V(.)O(2) kinetic parameters in endurance runners. Twelve endurance-trained runners [mean (SD) age 25.2 (4.7) years, body mass 70.1 (9.7) kg, height 179.5 (7.5) cm, ventilatory threshold (V(T)), 3,429 (389) ml.min(-1), maximal V(.)O(2) (V(.)O(2max)) 4,138 (625) ml.min(-1)] performed two multiple square-wave transition protocols on separate days. The protocol consisted of six (three transitions, 15 min rest, three transitions) square-wave transitions from walking at 4 km.h(-1) to running at a speed equivalent to 80% of the V(.)O(2) at the V(T) (80%V(T)). To determine the reproducibility, the protocol was repeated on a separate day (i.e. a test-retest design). Pulmonary gas-exchange was measured breath-by-breath. The V(.)O(2) data were modelled [from 20 s post-onset (or offset) of exercise] using non-linear least squares regression by a mono-exponential model, incorporating a time delay. The on- and off-transient time constants (tau(on) and tau(off)), mean response times (MRT(on) and MRT(off)) and amplitudes (A(on) and A(off)) were obtained from the model fit. On- and off transient kinetics were compared using paired t-tests. The reproducibility of each kinetic parameter was explored using statistical (paired t-tests) and non-statistical techniques [95% limits of agreement (LOA, including measurement error and systematic bias) and coefficient of variation (CV)]. It was found that the tau(on) [12.4 (1.9)] was significantly (P<0.001) shorter than tau(off) [24.5 (2.3) s]. Similarly, MRT(on) [27.1 (1.9) s] was shorter than MRT(off) [33.4 (2.2) s]. With respect to the reproducibility of the parameters, paired t-tests did not reveal significant differences between test 1 and test 2 for any on- or off-transient V(.)O(2) kinetic parameter (P>0.05). The LOA for tau(on) (1.9 s), tau(off) (2.3 s), MRT(on) (1.2 s), MRT(off) (3.2 s), A(on) (204 ml.min(-1)) and A(off) (198 ml.min(-1)) were narrow and acceptable. Furthermore, the measurement error (range, 4.3 to 15.1%) and CV (1.3 to 4.8%) all indicated good reproducibility. There was a tendency for tau(off) to be more reproducible than tau(on). However, MRT(on) was the most reproducible kinetic parameter. Overall, the results suggest that: (1) a multiple square-wave transition protocol can be used to characterise, reproducibly, both on- and off-transient V(.)O(2) kinetic parameters during treadmill running in runners; (2) the phase II time constant is independent of V(.)O(2) (max), and (3) asymmetry exists between on- and off transient V(.)O(2) kinetic parameters.
Kilding
A E
AE
Division of Sport and Recreation, Auckland University of Technology, Private Bag 92006, 1020 Auckland , New Zealand. andrew.kilding@aut.ac.nz
Challis
N V
NV
Winter
E M
EM
Fysh
M
M
D000328:Adult; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000502:physiology; D008297:Male; D008962:Models, Theoretical; D010101:Oxygen Consumption; D010807:Physical Endurance / Q000502:physiology*; D015203:Reproducibility of Results; D012420:Running / Q000502:physiology*