Technology for high-throughput screens: the present and future using zebrafish.
The zebrafish is a popular vertebrate model organism with similar organ systems and gene sequences to humans. Zebrafish embryos are optically transparent enabling organ visualisation, which can be complemented with gene expression analysis at the transcript and protein levels. Furthermore, zebrafish can be treated with small molecules and drugs in a microtitre plate format for high-throughput analysis and for the identification and validation of drugs. High-throughput methodologies for use in zebrafish include phenotype-based visualisation, transcript studies using low-density DNA microarrays and proteomic analysis. These technologies offer significant whole-organism biological value in the drug discovery and drug development pipeline.
Love
Donald R
DR
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1001, New Zealand. d.love@auckland.ac.nz
Pichler
Franz B
FB
Dodd
Andrew
A
Copp
Brent R
BR
Greenwood
David R
DR
D000818:Animals; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction; D040901:Proteomics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000502:physiology