title: Printed covalent glycan array for ligand profiling of diverse glycan binding proteins.
abstract: Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.
authors.affiliation: Glycan Synthesis and Protein Expression Core-D, Consortium for Functional Glycomics, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. olablixt@scripps.edu
authors.forename: Ola
authors.initials: O
authors.lastname: Blixt
authors.forename: Steve
authors.initials: S
authors.lastname: Head
authors.forename: Tony
authors.initials: T
authors.lastname: Mondala
authors.forename: Christopher
authors.initials: C
authors.lastname: Scanlan
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Huflejt
authors.forename: Richard
authors.initials: R
authors.lastname: Alvarez
authors.forename: Marian C
authors.initials: MC
authors.lastname: Bryan
authors.forename: Fabio
authors.initials: F
authors.lastname: Fazio
authors.forename: Daniel
authors.initials: D
authors.lastname: Calarese
authors.forename: James
authors.initials: J
authors.lastname: Stevens
authors.forename: Nahid
authors.initials: N
authors.lastname: Razi
authors.forename: David J
authors.initials: DJ
authors.lastname: Stevens
authors.forename: John J
authors.initials: JJ
authors.lastname: Skehel
authors.forename: Irma
authors.initials: I
authors.lastname: van Die
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Burton
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: Richard
authors.initials: R
authors.lastname: Cummings
authors.forename: Nicolai
authors.initials: N
authors.lastname: Bovin
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
mesh_terms: D002240:Carbohydrate Sequence; D003432:Cross-Linking Reagents; D008024:Ligands; D011134:Polysaccharides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012371:Robotics
chemical_list: D003432:Cross-Linking Reagents; D008024:Ligands; D011134:Polysaccharides; D011506:Proteins