title: Genetic diversity in clinical isolates of Mycobacterium avium complex from Guinea-Bissau, West Africa.
abstract: Isolates of Mycobacterium avium complex (MAC) were cultured from sputum samples obtained from patients in Guinea-Bissau, West Africa. Twenty-eight isolates hybridising with MAC probe (AccuProbe) were further characterised by different molecular techniques: hybridisation with species-specific probes (AccuProbe) for M. avium and M. intracellulare, partial sequencing of 16S rRNA gene and PCR detection of the DT1-DT6 sequences and the macrophage-induced gene (mig). Only one of the 28 isolates reacted with the M. avium probe and four with the M. intracellulare probe. Two isolates expressed the DT1 sequence, and three the DT6. The mig was detected in 18 (64%) of the isolates. Sequencing of 16S rRNA had the greatest discriminative power of the typing methods applied, without strong correlation with any other technique. Clinical MAC isolates from Guinea-Bissau demonstrated a wide genetic diversity among the members of M. avium complex that might reflect on biotope variation.
authors.affiliation: Department of Bacteriology, Swedish Institute for Infectious Disease Control, Nobels väg 18, SE-17182 Solna, Sweden. tuija.koivula@smi.ki.se
authors.forename: Tuija
authors.initials: T
authors.lastname: Koivula
authors.forename: Mariana
authors.initials: M
authors.lastname: Cristea-Fernström
authors.forename: Erja
authors.initials: E
authors.lastname: Chryssanthou
authors.forename: Björn
authors.initials: B
authors.lastname: Petrini
authors.forename: Gunilla
authors.initials: G
authors.lastname: Källenius
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D014644:Genetic Variation; D006169:Guinea-Bissau; D006801:Humans; D017720:Molecular Epidemiology; D015269:Mycobacterium avium Complex / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S