Identification and characterization of coiled body-like structures in pea (Pisum sativum L.).
Coiled bodies (CBs) are nuclear organelles which were considered as "universal" nuclear structures in eukaryotic cells, but the formation and function of CBs, especially in plant cells, remained unclear. In this article we reported that CBs in meristematic cells of pea are oval to round obstacles in nucleus and in adjacent to nucleolus, often have the same electron density with nucleolus. We found that CBs could be stained by the rRNP preference staining method, but no rDNA was detected in the structure. Furthermore, our results of immunoelectron microscopy showed that several processing factors, include fibrillarin, U3 snoRNA and ITS1, were present in CB. It seems probable that CBs is derived structurally from nucleolus and act as transport, storage and processing subnucleolar organelles.
Long
Hong
H
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, PR China.
Sun
Haijing
H
Zeng
Xianlu
X
Hao
Shui
S
Jiao
Mingda
M
D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020541:Coiled Bodies / Q000378:metabolism / Q000648:ultrastructure*; D018744:DNA, Plant / Q000378:metabolism; D004275:DNA, Ribosomal / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D018532:Pisum sativum / Q000378:metabolism / Q000648:ultrastructure*; D018749:RNA, Plant / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D018744:DNA, Plant; D004275:DNA, Ribosomal; D018749:RNA, Plant; D020537:RNA, Small Nucleolar; C117378:RNA, U3 small nucleolar; D012261:Ribonucleoproteins; C047458:fibrillarin