Synthesis and application of molecularly imprinted polymer on selective solid-phase extraction for the determination of monosulfuron residue in soil.
A novel sample clean-up procedure using molecularly imprinted polymer as the solid-phase extraction material for the determination of monosulfuron residue in soil samples has been developed. The molecularly imprinted polymer (MIP) was synthesized by non-covalent method with monosulfuron as the template. The selectivity and affinity of the MIP was evaluated by equilibrium adsorption and HPLC experiments, which demonstrated that the MIP has specific affinity for the template. The template-MIP interaction was studied by investigating the influence of different mobile phases on the retention of the template, which provided basic knowledge for the selection of the washing and elution solutions in the molecularly imprinted solid-phase extraction (MISPE) process. The study indicated that polar organic solvents with hydrogen bonding abilities have stronger eluting strength for the monosulfuron. After the MISPE procedure, a clean baseline was obtained in the HPLC quantification analysis. The recoveries of the method using the combination of MISPE and HPLC were above 93% and the R.S.D. was less than 3.2% in the soil sample determinations. Low detection limit (0.08 microg g(-1), when defined as 3 times of the noise) was also obtained in the method evaluation study.
Dong
Xiangchao
X
Department of Chemistry, Nankai University, Tianjin 300071, China. xcdong@nankai.edu.cn
Wang
Ning
N
Wang
Shengli
S
Zhang
Xuewei
X
Fan
Zhijin
Z
D002851:Chromatography, High Pressure Liquid; D011108:Polymers / Q000737:chemistry*; D011743:Pyrimidines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis*; D013453:Sulfonylurea Compounds / Q000032:analysis*
D011108:Polymers; D011743:Pyrimidines; D012989:Soil Pollutants; D013453:Sulfonylurea Compounds; C495688:monosulfuron