IL-10 augments antibody production in in vitro immunized lymphocytes by inducing a Th2-type response and B cell maturation.
An in vitro immunization (IVI) protocol enables antigen specific antibody production from L-Leucyl-L-Leucine methyl ester (LLME)-treated human peripheral blood lymphocytes (PBL) upon antigen stimulation in the presence of IL-2, IL-4, and muramyl dipeptide. In the course of our studies, we have found that IL-10 added at the antigen sensitization significantly augmented antibody production level from the LLME-treated PBL. In the present study, we tried to demonstrate the role of IL-10 in the augmentation of antibody production in an IVI protocol by clarifying the cytokine expression profiles in CD4(+) and CD8(+) T cells. The results showed that IL-10 skewed the Th1/Th2 balance to Th2-type responses by suppressing Th1-type cytokine production and augmenting Th2-type cytokine production in CD4(+) and CD8(+) T cells, as well as in CD19(+) B cells. Furthermore, IL-10 augmented the expression of CD38, an antigen marker of plasma cells, on B cells, which clearly indicates that IL-10 promoted differentiation and maturation of B cells in an IVI protocol. These results indicate that IL-10 plays an important role in setting the cellular milieu to produce antibodies in an IVI protocol.
Xu
Qianghua
Q
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Katakura
Yoshinori
Y
Yamashita
Makiko
M
Fang
Shengguo
S
Tamura
Takashi
T
Matsumoto
Shin-Ei
SE
Aiba
Yoshihiro
Y
Teruya
Kiichiro
K
Osada
Kazuhiro
K
Nishikawa
Ryuhei
R
Shirahata
Sanetaka
S
D000276:Adjuvants, Immunologic; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003584:Cytological Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D016753:Interleukin-10 / Q000494:pharmacology*; D008280:Magnetics; D051379:Mice; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*
D000276:Adjuvants, Immunologic; D016753:Interleukin-10