title: Detection of herpesvirus DNAs in perilymph obtained from patients with sensorineural hearing loss by real-time polymerase chain reaction.
abstract: OBJECTIVE
  Perilymph and peripheral blood mononuclear cells (PBMCs) from patients with bilateral severe sensorineural hearing loss (SNHL) were evaluated for the presence of DNA from cytomegalovirus (CMV), herpes simplex virus (HSV), and human herpesvirus (HHV)6.
  
  
  METHODS
  A prospective clinical study.
  
  
  METHODS
  The subjects were 14 patients who underwent cochlear implantation and 1 patient who underwent gentamicin injection in the inner ear. We attempted to detect viral DNA from perilymph and PBMCs by real-time polymerase chain reaction (rtPCR).
  
  
  RESULTS
  CMV DNA was detected in two perilymph specimens obtained from patients who were diagnosed as congenitally symptomatic CMV infection, although no CMV DNA was detected in PBMCs. Neither HSV DNA nor HHV6 DNA was detected in any other perilymph specimens. CMV DNA was detected in three PBMC samples, HSV DNA was detected in two samples, and HHV6 DNA was detected in six samples.
  
  
  CONCLUSIONS
  CMV may persistently infect the inner ear of patients with congenital CMV infection, and rtPCR analysis may prove to be a valuable tool for investigating the etiology of SNHL.
authors.affiliation: Department of Otolaryngology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan. ksugiura@katch.ne.jp
authors.forename: Saiko
authors.initials: S
authors.lastname: Sugiura
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Morishita
authors.forename: Eisuke
authors.initials: E
authors.lastname: Sato
authors.forename: Reiko
authors.initials: R
authors.lastname: Beppu
authors.forename: Taku
authors.initials: T
authors.lastname: Hattori
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakashima
mesh_terms: D000328:Adult; D001483:Base Sequence; D002675:Child, Preschool; D015897:Comorbidity; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006319:Hearing Loss, Sensorineural / Q000453:epidemiology / Q000821:virology*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000453:epidemiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010498:Perilymph / Q000821:virology; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral