[Construction and identification of anti-chymopapain scFv phage display library].
OBJECTIVE
To construct phage display library of anti-chymopapain scFv.


METHODS
V(H) and V(L) gene repertoires were amplified from splenocyte mRNA by RT-PCR and joined by a (Gly(4)ser)3 linker to obtain scFv genes. The scFv genes were then cloned into phagemid pFAB5C to construct phage display library. Affinity selection and ELISA were used to identify specific phage antibody to chymopapain.


RESULTS
After 4 rounds of panning, high affinity scFv was obtained.


CONCLUSIONS
Phage display library of anti-chymopapain scFv was successfully constructed, and scFv with binding ability to chymopapain was obtained.
Wang
Jian-ping
JP
Department of Bioengineering, South China University of Technology, Guangzhou 510640, China. bestwjp@sohu.com
Zhao
Shu-jin
SJ
Wang
Jie
J
Wu
Jie
J
Yang
Tai-cheng
TC
Yang
Jing
J
D000818:Animals; D002917:Chymopapain / Q000276:immunology* / Q000378:metabolism; D005260:Female; D050438:Genes, Immunoglobulin Heavy Chain; D050439:Genes, Immunoglobulin Light Chain; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D011485:Protein Binding
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D002917:Chymopapain