Acetohydroxamate inhibition of the activity of urease from dehusked seeds of water melon (Citrullus vulgaris).
Urease from the seeds of watermelon (Citrullus vulgaris) was purified to apparent homogeneity, using two acetone fractionation steps, heat treatment at 48 degrees C and gel filtration through Sephadex G-200. Effect of acetohydroxamic acid (AHA) on the activity of the homogeneous enzyme preparation (sp. act. 3000 +/- 550U/mg protein) was investigated. AHA exhibited a concentration-dependent inhibition both in the presence and absence of the substrate. The inhibition was uncompetitive and the Ki was 2.5 mM. Binding of AHA with the enzyme was reversible, as 63% activity could be restored by dialysis. Time-dependent inhibition revealed a monophasic inhibition of the activity. Addition of beta-mercaptoethanol (ME) gradually abolished the inhibition. Pre-treatment of native enzyme with 8.0 mM ME for 5 min at 30 degrees C exhibited protection against AHA-induced inhibition. The significance of these observations is discussed.
Prakash
Om
O
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. oprakash@bhu.ac.in
Upadhyay
Lata Sheo Bachan
LS
D029773:Citrullus / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D012639:Seeds / Q000201:enzymology*; D013379:Substrate Specificity; D014510:Urease / Q000037:antagonists & inhibitors* / Q000302:isolation & purification
D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; C006358:acetohydroxamic acid; D014510:Urease