Characterisation of a secondary alcohol dehydrogenase from Xanthomonas campestris DSM 3586.
The chromosomal locus NP_636946 of Xanthomonas campestris DSM 3586 (ATCC 33913) which was earlier presumed to encode a quinoprotein glucose dehydrogenase has been cloned, expressed in Escherichia coli and the recombinant enzyme has been characterised. It was found to have no glucose dehydrogenase activity but to be active on many different polyols and diols, aliphatic alcohols, certain aldonic acids and amino-sugars. The product of D: -gluconic acid oxidation was 5-keto-D: -gluconic acid. The enzyme differs from polyol/gluconate dehydrogenases found in Gluconobacter by its single-chain architecture, different substrate specificity and much higher (20- to 30-fold) expression level in E.coli.
Salusjärvi
Tuomas
T
Danisco Innovation, Sokeritehtaantie 20, Kantvik, 02460, Finland.
Hvorslev
Niels
N
Miasnikov
Andrei N
AN
D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism; D000606:Amino Sugars / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005942:Gluconates / Q000378:metabolism; D020576:Gluconobacter; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D016959:Xanthomonas campestris / Q000201:enzymology*
D000438:Alcohols; D000606:Amino Sugars; D001426:Bacterial Proteins; D005942:Gluconates; D011994:Recombinant Proteins; C093583:5-ketogluconic acid; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP); C030691:gluconic acid