title: The etiological agent of Lyme disease, Borrelia burgdorferi, appears to contain only a few small RNA molecules.
abstract: Small regulatory RNAs (sRNAs) have recently been shown to be the main controllers of several regulatory pathways. The function of sRNAs depends in many cases on the RNA-binding protein Hfq, especially for sRNAs with an antisense function. In this study, the genome of Borrelia burgdorferi was subjected to different searches for sRNAs, including direct homology and comparative genomics searches and ortholog- and annotation-based search strategies. Two new sRNAs were found, one of which showed complementarity to the rpoS region, which it possibly controls by an antisense mechanism. The role of the other sRNA is unknown, although observed complementarities against particular mRNA sequences suggest an antisense mechanism. We suggest that the low level of sRNAs observed in B. burgdorferi is at least partly due to the presumed lack of both functional Hfq protein and RNase E activity.
authors.affiliation: Department of Molecular Biology, Umeå University, SE-901 87, Umeå, Sweden.
authors.forename: Yngve
authors.initials: Y
authors.lastname: Ostberg
authors.forename: Ignas
authors.initials: I
authors.lastname: Bunikis
authors.forename: Sven
authors.initials: S
authors.lastname: Bergström
authors.forename: Jörgen
authors.initials: J
authors.lastname: Johansson
mesh_terms: D000818:Animals; D025065:Borrelia burgdorferi / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D004722:Endoribonucleases; D016680:Genome, Bacterial; D023281:Genomics; D035001:Host Factor 1 Protein; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology*; D035683:MicroRNAs / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics*; D011817:Rabbits
chemical_list: D035001:Host Factor 1 Protein; D035683:MicroRNAs; D012329:RNA, Bacterial; D004722:Endoribonucleases; C022537:ribonuclease E