Nutritional assessment and fate of DNA of soybean meal from roundup ready or conventional soybeans using rats.
This study was conducted to compare the safety of soybean meal prepared from genetically modified (GM) glyphosate-tolerant (Roundup Ready; RR) soybeans and conventional soybeans. Eighty Sprague-Dawley rats (40 males and 40 females) were randomly allotted to one of four groups according to sex and body weight for a 13-week feeding experiment. The rats were fed corn-based diets containing 60% conventional soybean meal, a mixture of 30% conventional and 30% RR soybean meal, 60% or 90% RR soybean meal. All diets were adjusted to an identical nutrient level except the 90% RR diet. The two soybean meals were similar in chemical analysis and amino acid composition. During the 13-week growth trial, body weight (P < 0.05) and feed intake (P < 0.05) decreased only in rats fed with 90% RR soybean meal at the first week. No treatment-related deaths occurred during the experiment. Gross necropsy findings, haematological or urinalysis values and clinical serum parameters showed no meaningful differences between rats fed the control and RR soybean meals. A 145 bp of cp4 epsps gene specific for the GM constructs from RR soybean meal or a 407 bp of lec gene from endogenous soybean DNA could not be detected in investigated masseter muscle samples. No adverse effects of glyphosate-tolerant soybean meal on rats were seen even at levels as high as 90% of the diet.
Zhu
Yuanzhao
Y
National Feed Engineering Technology Research Center, China Agricultural University, Beijing, China.
Li
Defa
D
Wang
Fenglai
F
Yin
Jingdong
J
Jin
Hong
H
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D018744:DNA, Plant / Q000032:analysis* / Q000097:blood / Q000652:urine; D004305:Dose-Response Relationship, Drug; D002149:Energy Intake / Q000187:drug effects; D005260:Female; D008297:Male; D015596:Nutrition Assessment; D030821:Plants, Genetically Modified / Q000009:adverse effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000254:growth & development*; D012449:Safety; D030262:Soybean Proteins / Q000008:administration & dosage* / Q000235:genetics; D013025:Glycine max / Q000235:genetics*; D016482:Urinalysis / Q000662:veterinary; D015430:Weight Gain / Q000187:drug effects
D018744:DNA, Plant; D030262:Soybean Proteins