Quality control of Harpagophytum procumbens and its related phytopharmaceutical products by means of NIR-FT-Raman spectroscopy.
NIR-FT-Raman spectroscopy was used for identification and quantification of harpagoside in secondary roots of Harpagophytum procumbens as well as in related phytopharmaceutical products, e.g., ethanolic extracts and tablets. Applied Raman mappings reveal the spatial distribution of this valuable iridoid glycoside within the different samples. The same technique can be used for quality control purposes beginning from the plant to its final products. Based on the obtained spectral data and reference HPLC values of harpagoside, a reliable multivariate calibration model was developed.
Baranska
M
M
Federal Centre for Breeding Research on Cultivated Plants (BAZ), Institute for Plant Analysis, Neuer Weg 22-23, 06484 Quedlinburg, Germany.
Schulz
H
H
Siuda
R
R
Strehle
M A
MA
Rösch
P
P
Popp
J
J
Joubert
E
E
Manley
M
M
D002138:Calibration; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D031684:Harpagophytum / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013607:Tablets
D006027:Glycosides; D010936:Plant Extracts; D011714:Pyrans; D013607:Tablets; C033249:harpagoside