title: Binary hammerhead ribozymes with high cleavage activity.
abstract: Binary hammerhead ribozymes consisted of two oligoribonucleotides capable of assembling into hammerhead structure (without loop II) on the RNA target were engineered. Catalytic activities of such ribozymes were investigated in comparison with their full-length analog and ribozyme where two strands were jointed by non-nucleotidic linker. Binary constructs were shown to be significantly more active than the parent full-length hammerhead ribozyme.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
authors.forename: M
authors.initials: M
authors.lastname: Kuznetsova
authors.forename: D
authors.initials: D
authors.lastname: Novopashina
authors.forename: M
authors.initials: M
authors.lastname: Repkova
authors.forename: A
authors.initials: A
authors.lastname: Venyaminova
authors.forename: V
authors.initials: V
authors.lastname: Vlassov
mesh_terms: D002384:Catalysis; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*
chemical_list: D016337:RNA, Catalytic