Toward the development of metal-free synthetic nucleases: cleavage of a model substrates by 1,4-diazabicyclo[2.2.2]octane derivatives.
Artificial ribonucleases of A(n)BCL series were synthesized by solid-phase method. They consist of a hydrophobic alkyl radical A (n = 3-12 carbon atoms), an "RNA-binding domain" B (bisquaternary salt of 1,4-diazabicyclo[2.2.2]octane), a "catalytic domain" C (histidine residue) and a "linker" L that joins the domains B and C. The effect of the alkyl radical on the catalytic properties of the chemical catalyst was studied using three activated phosphate ester substrates: p-nitrophenyl phosphate, bis-p-nitrophenyl phosphate, and thymidine-3'-p-nitrophenyl phosphate.
Koroleva
Lyudmila S
LS
Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, Russia. kls_2002@mail.ru
Silnikov
Vladimir N
VN
D003851:Deoxyribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D010879:Piperazines / Q000737:chemistry*; D013379:Substrate Specificity
D010879:Piperazines; D003851:Deoxyribonucleases; C007306:triethylenediamine