Selective assembly of HIV-1 Vif-Cul5-ElonginB-ElonginC E3 ubiquitin ligase complex through a novel SOCS box and upstream cysteines.
APOBEC3G, which induces hypermutations in newly synthesized viral DNA, is suppressed by HIV-1 Vif, acting through Cul5-ElonginB-ElonginC E3 ubiquitin ligase. We have now characterized a novel SOCS box in HIV-1 Vif that mediates its interaction with ElonginC. In this SOCS box, alanine replaces the consensus cysteine in the previously identified SOCS box. This new motif was necessary but insufficient for interaction with Cul5-ElonginB-ElonginC, as two highly conserved Cys residues outside the SOCS box were required to interact with Cul5 but not ElonginC. Therefore, selective assembly with Cul5 versus Cul2 E3 may require protein interfaces besides the SOCS-box-ElonginC interaction.
Yu
Yunkai
Y
Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Xiao
Zuoxiang
Z
Ehrlich
Elana S
ES
Yu
Xianghui
X
Yu
Xiao-Fang
XF
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D044842:Cullin Proteins / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism*; D017931:DNA Primers; D000075065:Elongin; D016346:Gene Products, vif / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D010957:Plasmids; D012097:Repressor Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism*; D054320:vif Gene Products, Human Immunodeficiency Virus
D044842:Cullin Proteins; D017931:DNA Primers; D000075065:Elongin; D016346:Gene Products, vif; D012097:Repressor Proteins; D014157:Transcription Factors; D054320:vif Gene Products, Human Immunodeficiency Virus; D003545:Cysteine