title: Oxalate absorption and endogenous oxalate synthesis from ascorbate in calcium oxalate stone formers and non-stone formers.
abstract: BACKGROUND
  Increased rates of either oxalate absorption or endogenous oxalate synthesis can contribute to hyperoxaluria, a primary risk factor for the formation of calcium oxalate-containing kidney stones. This study involves a comparative assessment of oxalate absorption and endogenous oxalate synthesis in subpopulations of stone formers (SFs) and non-stone formers (NSFs) and an assessment of the effect of ascorbate supplementation on oxalate absorption and endogenous oxalate synthesis.
  
  
  METHODS
  Twenty-nine individuals with a history of calcium oxalate kidney stones (19 men, 10 women) and 19 age-matched NSFs (8 men, 11 women) participated in two 6-day controlled feeding experimental periods: ascorbate-supplement (2 g/d) and no-supplement treatments. An oxalate load consisting of 118 mg of unlabeled oxalate and 18 mg of 13C2 -oxalic acid was administered the morning of day 6 of each experimental period.
  
  
  RESULTS
  Mean 13C2 -oxalic acid absorption averaged across the ascorbate and no-supplement treatments was significantly greater in SFs (9.9%) than NSFs (8.0%). SFs also had significantly greater 24-hour post-oxalate load urinary total oxalate and endogenous oxalate levels with both treatments. Twenty-four-hour urinary total oxalate level correlated strongly with both 13C2 -oxalic acid absorption (SFs, r = 0.76; P < 0.01; NSFs, r = 0.62; P < 0.01) and endogenous oxalate synthesis (SFs, r = 0.95; P < 0.01; NSFs, r = 0.92; P < 0.01).
  
  
  CONCLUSIONS
  SFs are characterized by greater rates of both oxalate absorption and endogenous oxalate synthesis, and both these factors contribute to the hyperoxaluric state. The finding that ascorbate supplementation increased urinary total and endogenous oxalate levels suggested that this practice is a risk factor for individuals predisposed to kidney stones.
authors.affiliation: Department of Family and Consumer Sciences (Nutrition), University of Wyoming, Laramie, WY 82071, USA.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Chai
authors.forename: Michael
authors.initials: M
authors.lastname: Liebman
authors.forename: Susan
authors.initials: S
authors.lastname: Kynast-Gales
authors.forename: Linda
authors.initials: L
authors.lastname: Massey
mesh_terms: D000328:Adult; D000368:Aged; D001205:Ascorbic Acid / Q000378:metabolism*; D002129:Calcium Oxalate / Q000378:metabolism*; D005260:Female; D006801:Humans; D006959:Hyperoxaluria / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007669:Kidney Calculi / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010070:Oxalates / Q000378:metabolism*
chemical_list: D010070:Oxalates; D002129:Calcium Oxalate; D001205:Ascorbic Acid