Restoration of axotomy-induced PACAP gene induction in SCID mice with CD4+ T-lymphocytes.
PACAP is a neuropeptide with putative neuroprotective, regenerative, and immunomodulatory actions. PACAP mRNA is up-regulated in motor neurons following facial nerve axotomy in wild type, but not immunodeficient SCID mice. Because CD4+ lymphocytes appear to be neuroprotective in facial nerve and other injury models, we studied PACAP gene expression in SCID mice preinfused with CD4+ enriched splenocytes. Whereas the mean number of PACAP hybridizing neurons after axotomy was reduced by 75% in uninfused SCID mice, infusion of CD4+ enriched splenocytes restored the number to a value not significantly different than controls. The CD4+ cell-dependent induction of PACAP in motor neurons may thus be a factor in the cascade of events triggered by immune cells that ultimately lead to nerve regeneration.
Armstrong
Brian D
BD
University of California at Los Angeles, 760 Westwood Plaza, NPI 68-225, Los Angeles, CA 90024, USA.
Abad
Catalina
C
Chhith
Seririthanar
S
Rodriguez
Williams
W
Cheung-Lau
Gardenia
G
Trinh
Van
V
Waschek
James A
JA
D000704:Analysis of Variance; D000818:Animals; D019771:Axotomy / Q000379:methods; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D005154:Facial Nerve / Q000166:cytology / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods; D007839:Functional Laterality / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D017403:In Situ Hybridization / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009046:Motor Neurons / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000235:genetics / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger / Q000378:metabolism; D015533:Transcriptional Activation
C495402:Adcyap1 protein, mouse; D009414:Nerve Growth Factors; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger