A selective, cell-permeable optical probe for hydrogen peroxide in living cells.
We present the synthesis, properties, and biological applications of Peroxyfluor-1 (PF1), a new type of optical probe for intracellular imaging of hydrogen peroxide in living biological samples. PF1 utilizes a boronate deprotection mechanism to provide unprecedented selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical. We further demonstrate the value of this reagent for biological applications by imaging changes in [H2O2] in living mammalian cells.
Chang
Michelle C Y
MC
Departments of Chemistry and Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Pralle
Arnd
A
Isacoff
Ehud Y
EY
Chang
Christopher J
CJ
D001897:Boronic Acids / Q000737:chemistry; D002460:Cell Line; D002463:Cell Membrane Permeability; D004952:Esters / Q000737:chemistry; D005452:Fluoresceins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D013379:Substrate Specificity; D014966:Xanthenes / Q000737:chemistry
D001897:Boronic Acids; D004952:Esters; D005452:Fluoresceins; D014966:Xanthenes; C500013:peroxyfluor-1; D006861:Hydrogen Peroxide