Alkylation and cleavage of a DNA fragment with trioligonucleotide reagent.
A possibility of site-directed chemical modification of a ssDNA fragment with "trioligonucleotide reagent" (TOR), consisting of a central oligonucleotide derivative carrying N-(2-chloroethyl)-N-(p-formylphenyl)-N-propyl-N-3-ydeneamino groups at both 5'- and 3'-thiophosphate ends and two border derivatives with 4-carbohydrazidephenyl groups at their 3'- and 5'-phosphate ends, respectively, is shown. Products of site-directed fragment cleavage, more abundant than the alkylation products, were found at 50 degrees C. The overall level of DNA modification by TOR reached 30% at a small excess of the oligonucleotide derivatives.
Oshevski
S I
SI
Institute of Cytology and Genetics of the Russian Academy of Sciences, Novosibirsk, Russia. oshevski@bionet.nsc.ru
D000478:Alkylation; D001345:Autoradiography; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D006868:Hydrolysis; D007202:Indicators and Reagents / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*
D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009841:Oligonucleotides