[Improvement of the model of pancreas-duodenal transplantation in swines].
OBJECTIVE
To improve the procedures of pancreas-duodenal transplantation in swines in order to establish a technically stable model that accords with the physiological situation.


METHODS
The whole pancreatoduodenal graft was harvested with in situ flush using 4 degrees C preservation solution. Type 1 diabetes was induced surgically by total pancreatectomy. By end-to-side fashion, the segment of abdominal aorta containing the celiac artery and the anterior mesenteric artery (equal to superior mesenteric artery in human) was anastomosed to the abdominal aorta of recipient which is behind the renal artery, the portal vein was anastomosed to the anterior mesenteric vein of the recipient, the duodenum was anastomosed to the jejunum of the recipient. The graft was placed in the anterior right peritoneal cavity.


RESULTS
A total of 28 swines were used as recipients. Of them, two died of anesthetic accident, another two died of hyperacute rejection 2 hours, 19 hours after operation respectively; the others underwent the operation uneventfully. Their survival time was 5-21 days, blood sugar was (11.33+/-1.66) mmol/L on the first day after operation, and (5.65+/-0.89) mmol/L on the third day.


CONCLUSIONS
This kind of model is stable, and the operative method and procedures are relatively simple and practicable.
Tang
Yong
Y
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Zhao-da
ZD
Wei
Jiang-Tao
JT
Hou
Ming-yong
MY
Li
Jian-shui
JS
Jiang
Jun-ming
JM
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D004386:Duodenum / Q000601:surgery / Q000637:transplantation*; D005260:Female; D006085:Graft Survival; D008297:Male; D023421:Models, Animal; D016035:Pancreas Transplantation / Q000379:methods*; D013552:Swine; D014184:Transplantation, Homologous