[Preparation and characterization of the monoclonal antibody against HIV-1 p24 antigen].
OBJECTIVE
To establish hybridoma cells secreting monoclonal antibodies (mAbs) against HIV-1 p24 antigen and characterize their properties.


METHODS
BALB/c mice were immunized with purified p24 protein and then the from splenocytes immunized mice were fused with Sp2/0 cells. Monoclonal hybridoma cell lines were obtained by limiting dilution, HAT and HT-selective culture. The specificity of mAbs was identified by Dot blot and indirect ELISA.


RESULTS
We had established two hybridoma cell lines secreting mAbs against HIV-1 p24 antigen. The titers of two mAbs in ascitic fluid were 1 x 10(-5) and 1.7 x 10(4)-1.8 x 10(4) mol/L, respectively. Both mAbs belonged to IgG1. Indirect ELISA detection demonstrated that both mAbs had no cross-reactivities with other viral antigens, such as HBcAg, HCV RNA positive sera and HIVgp41.


CONCLUSIONS
Two hybridoma cell lines secreting mAbs against HIV-1 p24 antigen have been established successfully, which lays the foundation for detecting HIV-1 p24 antigen.
Hou
Jun
J
Department of Virology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. lba@263.net
Hu
Yan
Y
Zhu
Lei
L
Shen
Hong-hui
HH
Yang
Jian-yang
JY
Hong
Shi-wen
SW
Mao
Pan-yong
PY
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D002459:Cell Fusion; D045744:Cell Line, Tumor; D002469:Cell Separation; D016655:HIV Core Protein p24 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D016655:HIV Core Protein p24