[Establishment and characteristics of orthotopically transplanted model of human primary malignant small intestinal lymphoma in nude mice].
OBJECTIVE
To establish orthotopically transplanted model of human malignant small intestinal lymphoma in nude mice and analyze their biologic characteristics.


METHODS
Small intestinal lymphoma tissues from 5 patients were transplanted into intestinal mucosa of nude mice. Tumorgenecity, invasion and metastasis of the transplanted tumors were observed by morphological analyses (light microscopy, electron microscopy and immunohistochemistry), karyotyping and DNA quantitative assay.


RESULTS
Tumor tissues from 3 lymphoma patients were successfully transplanted. According to the World Health Organization classification, the three models were classified into non-Hodgkin's lymphoma (B cell) of human small intestine (HSIL-1), high metastasis of non-Hodgkin's lymphoma (B cell) of human small intestine (HSIL-2) and non-Hodgkin's lymphoma (T cell) of human small intestine (HSIL-3), respectively. Immunohistochemistry showed that CD19, CD20, CD22, CD40, CD45 and CD72 were positive in HSIL-1 and HSIL-2, whereas CD3, CD7 and CD45RO were positive in HSIL-3. The karyotypes of the transplanted tumors were all hypotriploid with modal numbers from 55 to 69 and the DNA index (DI) was 1.46 approximately 1.71. The three models had been passaged for 32, 27 and 21 generations respectively in 433 nude mice. The growth rate, resuscitation rate of the liquid nitrogen preserved tumor cells and spontaneous metastasis rate upon transplantation were all 100%. We observed an invasive growth of the transplanted tumors in small intestine, which resulted in disrupting of the intestinal wall, hematogenous metastasis, lymph node metastasis and seeding metastasis. The features of the transplanted tumors were similar to the original tumors in histopathology, ultrastructure, DNA content and karyotype.


CONCLUSIONS
Three strains of orthotopically transplanted model of human primary malignant small intestinal lymphoma in nude mice were successfully developed. The result of research will provide ideal animal models for further studies on mechanism of tumorigenesis, invasion and metastasis of malignant small intestinal lymphoma and experimental therapy.
Liu
Qiu-Zhen
QZ
Department of Hepatobiliary Surgery, No.202 Hospital of PLA, Shenyang 110003, China.
Tuo
Chao-Wei
CW
Zhang
Ning
N
Wang
Ming-Yao
MY
D000328:Adult; D000782:Aneuploidy; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007414:Intestinal Neoplasms / Q000276:immunology / Q000473:pathology*; D007421:Intestine, Small / Q000473:pathology; D008113:Liver Neoplasms / Q000556:secondary; D008207:Lymphatic Metastasis; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D013160:Splenic Neoplasms / Q000556:secondary
D015703:Antigens, CD; D004273:DNA, Neoplasm