title: In vitro studies on the evaluation of mycotoxin detoxifying agents for their efficacy on deoxynivalenol and zearalenone.
abstract: A simple in vitro system was developed to study the efficacy of commercially available mycotoxin detoxifying agents and adsorbing substances as feed additives to detoxify deoxynivalenol (DON) and zearalenone (ZON) in situ. The in vitro model simulates the conditions (pH, temperature and transit time) of the porcine gastrointestinal tract, as pigs react most sensitively to these mycotoxins. The commercially available products were not effective in detoxifying DON and ZON under the applied conditions, while activated carbon was able to bind both toxins and cholestyramine, and a modified aluminosilicate showed good adsorption abilities for ZON. Data obtained in dose dependency studies showed an estimated adsorption capacity of cholestyramine and the modified aluminosilicate of 11.7 and 5.7 g ZON/kg detoxifying agent. The in vitro system deployed in the present study was demonstrated to be a simple, helpful tool in screening substances for their ability to detoxify DON and ZON under the simulated conditions of the porcine gastrointestinal tract. Nonetheless in vivo experiments are indispensable to proof the efficacy.
authors.affiliation: Institute of Animal Nutrition, Federal Agricultural Research Centre (FAL), Braunschweig, Germany. susanne.doell@fal.de
authors.forename: S
authors.initials: S
authors.lastname: Döll
authors.forename: S
authors.initials: S
authors.lastname: Dänicke
authors.forename: H
authors.initials: H
authors.lastname: Valenta
authors.forename: G
authors.initials: G
authors.lastname: Flachowsky
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis / Q000382:microbiology; D000818:Animals; D000837:Anion Exchange Resins / Q000378:metabolism; D002021:Buffers; D002606:Charcoal / Q000378:metabolism; D002792:Cholestyramine Resin / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000378:metabolism; D005503:Food Additives / Q000494:pharmacology*; D005506:Food Contamination / Q000517:prevention & control*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008954:Models, Biological; D009183:Mycotoxins / Q000008:administration & dosage / Q000378:metabolism*; D013552:Swine; D013997:Time Factors; D016896:Treatment Outcome; D014255:Trichothecenes / Q000008:administration & dosage / Q000378:metabolism; D015025:Zearalenone / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D000538:Aluminum Silicates; D000837:Anion Exchange Resins; D002021:Buffers; D004968:Estrogens, Non-Steroidal; D005503:Food Additives; D009183:Mycotoxins; D014255:Trichothecenes; D002792:Cholestyramine Resin; D002606:Charcoal; C049037:aluminosilicate; D015025:Zearalenone; C007262:deoxynivalenol