Gene expression profiles correlate with the morphology and metastasis characteristics of renal cell carcinoma cells.
Renal cell carcinoma (RCC) shows various clinical behaviors, and currently surgical modalities are the only effective therapy against this cancer. To discern the genomic background affecting clinical characteristics such as metastasis, we analyzed the gene expression profiles of the RCCs by DNA microarray using cell lines originating from primary or metastatic lesions. RNA was extracted from ten SKRC RCC cell lines and gene expression profiling was performed using a cDNA microarray of nearly 4,000 human cDNA clones. We compared the gene expression profiles between these SKRC cell lines and the normal renal proximal tubular cell line and among SKRC cell lines that showed different characteristics. The clustering of the selected 62 genes revealed similar profiling patterns between SKRC-17 and SKRC-29 cells that were derived from metastatic RCC lesions. Another cell line from a metastatic lesion, SKRC-52, with a unique spindle-shaped morphology, had a different pattern of expression profiling from the other cell lines. We found several genes up-regulated in the cell lines from metastatic lesions; transgelin, which is reportedly involved in cell proliferation and migration, was up-regulated in SKRC-52. The unique profiling pattern of gene expression clearly correlated with cell morphology and metastatic potential. Such profiling might contribute to identifying the genes involved in the clinical characteristics of RCC.
Ami
Yoshihiro
Y
Department of Biochemistry and Molecular Oncology, Comprehensive Human Science, Graduate School, University of Tsukuba, Ibaraki 305-8573, Japan.
Shimazui
Toru
T
Akaza
Hideyuki
H
Uematsu
Naoya
N
Yano
Yukiko
Y
Tsujimoto
Gozoh
G
Uchida
Kazuhiko
K
D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000473:pathology; D045744:Cell Line, Tumor; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation