Casein kinase 2 (CK2)-mediated reduction of the activities of Src family tyrosine kinases in vitro.
The physiological correlation between casein kinase 2 (CK2) and two Src family tyrosine kinases (Src-TKs, Fyn and Src) was mainly investigated in vitro. It was found that (i) Thr-residues of these two Src-TKs were preferentially phosphorylated by CK2 using [gamma-32P]GTP as a phosphate donor; (ii) this phosphorylation was highly stimulated in the presence of poly-Arg; (iii) full phosphorylation of two Src-TKs by CK2 resulted in significant reduction of their TK activities; and (iv) quercetin (a CK2 inhibitor) inhibited the CK2-mediated reduction of their Src-TK activities in vitro. Under the same experimental conditions, similar results were obtained with Yes. These results suggest that CK2 may be a protein kinase responsible for the suppression of at least three Src-TKs (Fyn, Src and Yes) through the specific phosphorylation of their Thr-residues at the cellular level.
Yokoyama
Takamasa
T
Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University.
Kamata
Yayoi
Y
Ohtsuki
Kenzo
K
D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
D004791:Enzyme Inhibitors; D019061:src-Family Kinases; D047390:Casein Kinase II