Production of an antiproliferative furanoheliangolide by Lychnophora ericoides cell culture.
This work reports for the first time the production a furanoheliangolide (goyazensolide) by plant cell culture. Monitoring of the goyazensolide metabolism revealed that the maximum production occurred during the lag phase of the Lychnophora ericoides callus culture. The antiproliferative activity of obtained goyazensolide was evaluated against seven cancer cell lines using MTT assay. The results revealed a potent cytotoxic activity for the furaheliangolide with IC50 values in the range of 0.06 microg/ml for CEM leukemia cells to 0.75 microg/ml for B16 melanome cells.
dos Santos
Pierre Alexandre
PA
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão, Preto-SP, Brazil.
Amarante
Maria Fernanda Castro
MF
Pereira
Ana Maria Soares
AM
Bertoni
Bianca
B
França
Suzelei Castro
SC
Pessoa
Cláudia
C
de Moraes
Manoel Odorico
MO
Costa-Lotufo
Letícia Veras
LV
Pereira
Marcio Roberto Pinho
MR
Lopes
Norberto Peporine
NP
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000737:chemistry; D019659:Asteraceae / Q000737:chemistry*; D001952:Bridged-Ring Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005047:Etoposide / Q000494:pharmacology; D005663:Furans / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D054830:Sesterterpenes; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013256:Steroids / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D001952:Bridged-Ring Compounds; D005663:Furans; D054830:Sesterterpenes; D013256:Steroids; C499803:furanoheliangolide; D005047:Etoposide; D004317:Doxorubicin