title: Crystallization and preliminary crystallographic analysis of the 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader Pseudomonas resinovorans strain CA10.
abstract: CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 A, alpha = beta = gamma = 90 degrees . The crystals diffracted to a maximum resolution of 1.9 A and gave a data set with an overall R(merge) of 5.7% and a completeness of 98.6%. The V(M) value was 2.52 A(3) Da(-1), which indicated a solvent content of 51.2%.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Iwata
authors.forename: Haruko
authors.initials: H
authors.lastname: Noguchi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Usami
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Nam
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D049308:Dioxygenases / Q000737:chemistry*; D006860:Hydrogen Bonding; D008956:Models, Chemical; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D011549:Pseudomonas / Q000201:enzymology*; D012997:Solvents; D017356:Synchrotrons; D014965:X-Rays
chemical_list: D012997:Solvents; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase