title: Use of coherent control methods through scattering biological tissue to achieve functional imaging.
abstract: We test whether coherent control methods based on ultrashort-pulse phase shaping can be applied when the laser light propagates through biological tissue. Our results demonstrate experimentally that the spectral-phase properties of shaped laser pulses optimized to achieve selective two-photon excitation survive as the laser pulses propagate through tissue. This observation is used to obtain functional images based on selective two-photon excitation of a pH-sensitive chromophore in a sample that is placed behind a slice of biological tissue. Our observation of coherent control through scattering tissue suggests possibilities in multiphoton-based imaging and photodynamic therapy.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Dela Cruz
authors.forename: Igor
authors.initials: I
authors.lastname: Pastirk
authors.forename: Matthew
authors.initials: M
authors.lastname: Comstock
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Lozovoy
authors.forename: Marcos
authors.initials: M
authors.lastname: Dantus
mesh_terms: D000818:Animals; D001190:Arylsulfonates; D003952:Diagnostic Imaging; D004867:Equipment Design; D006801:Humans; D007834:Lasers; D008892:Milk; D018482:Muscle, Skeletal; D012542:Scattering, Radiation
chemical_list: D001190:Arylsulfonates; C005047:pyranine