Simple method for large-scale isolation and purification of gangliosides by non-ionic adsorption chromatography.
A type of non-ionic adsorption resin, X-5, was used in the isolation and purification of brain gangliosides. Hydrolysis with base treatment was carried out for the purpose of eliminating contaminants. The major advantages of the new procedure, compared to conventional methods, were the shorter separation time, higher loading capacity (80 micromol LBSA per gram resin), and recovery (98%) of separated ganglioside fractions with little solvent. It is a practical way for large-scale isolation and purification of gangliosides.
Wang
Xuedong
X
State Key Laboratory of Bioreactor Engineering, Luhua Institute of Biochemistry, East China University of Science and Technology, Shanghai, P.R. China. wxdzhl@163.com
Wei
Dongzhi
D
Zhou
Lan
L
Yin
Zhe
Z
D000327:Adsorption; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods*; D005732:Gangliosides / Q000737:chemistry* / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry*; D013552:Swine; D014020:Tissue Extracts / Q000737:chemistry / Q000302:isolation & purification
D005732:Gangliosides; D012117:Resins, Synthetic; D014020:Tissue Extracts