[Significance of membrane type-1 matrix metalloproteinase expression in breast cancer].
OBJECTIVE
Recent researches manifested that MT1- MMP (Membrane type-1 matrix metalloproteinase) is essential for breast cancer invasion and metastasis, but there are few reports about the clinical value of MT1- MMP. This study was designed to investigate the clinical significance of MT1-MMP protein and mRNA expression in human breast cancers.


METHODS
Forty-six human breast cancer tissues were collected. MT1-MMP protein and mRNA expression were detected by semi-quantitative reverse transcriptase chain reaction (RT-PCR) and immunohistochemistry assay and their correlations with clinicopathological factors were analyzed.


RESULTS
The expression of MT1-MMP protein was positive in 52.2% of forty-six breast cancer tissues. The positive rates were 12.5%, 54.8% and 85.7% in stageI, stage II and stage III human breast cancers, respectively (P < 0.05), and 11.1%, 59.4% and 80.0% in the group of T(1), T(2) and T(3) (P < 0.05), and 27.3%, 66.7% and 100.0% in the group of N(0), N(1) and N(2) (P < 0.05). MT1-MMP mRNA expression were detected in all of the carcinomas. The means of MT1-MMP mRNA expression relative quantities were 0.4730, 0.6950 and 0.9100 in stageI, stage II and stage III human breast cancers, respectively (P < 0.05), and 0.5240, 0.7150 and 0.8220 in the group of T(1),T(2) and T(3) (P < 0.05), and 0.6305, 0.7022 and 0.8700 in the group of N(0), N(1) and N(2)(P < 0.05). There were no relations between the MT1-MMP protein or mRNA expression and ER, PR or C-erbB-2(P >0.05).


CONCLUSIONS
MT1-MMP protein and mRNA expression in human breast cancers had positive correlation with the stage,the tumor size and lymph node metastasis. MT1-MMP can be a predictor for the ability of breast cancer invasion and metastasis.
Yao
Guang-Yu
GY
Department of Thoracic Oncology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P.R.China.
Yang
Ming-Tian
MT
Rong
Tie-Hua
TH
He
Ping
P
D000328:Adult; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases