title: S-adenosyl-L-homocysteine hydrolase in yeast: key enzyme of methylation metabolism and coordinated regulation with phospholipid synthesis.
abstract: S-adenosyl-L-homocysteine hydrolase (Sah1p, EC 3.3.1.1.) is a key enzyme of methylation metabolism. It catabolizes S-adenosyl-L-homocysteine, which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor, and which acts as strong competitive inhibitor of all AdoMet-dependent methyltransferases. Sah1p is an essential enzyme in yeast and one of the most highly conserved proteins with up to 80% sequence homology throughout all kingdoms of life. SAH1 expression in yeast is subject to the general transcriptional control of phospholipid synthesis. Profound changes in cellular lipid composition upon depletion of Sah1p support the notion of a tight interaction between lipid metabolism and Sah1p function.
authors.affiliation: Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstrasse 1, 8010 Graz, Austria.
authors.forename: Oksana
authors.initials: O
authors.lastname: Tehlivets
authors.forename: Meinhard
authors.initials: M
authors.lastname: Hasslacher
authors.forename: Sepp D
authors.initials: SD
authors.lastname: Kohlwein
mesh_terms: D000595:Amino Acid Sequence; D016384:Consensus Sequence; D017124:Conserved Sequence; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005819:Genetic Markers; D006867:Hydrolases / Q000378:metabolism*; D008745:Methylation; D008954:Models, Biological; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D012435:S-Adenosylhomocysteine / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D005819:Genetic Markers; D010743:Phospholipids; D029701:Saccharomyces cerevisiae Proteins; D012435:S-Adenosylhomocysteine; D006867:Hydrolases