Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2.
We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
McConnell
Gail
G
Centre for Biophotonics, SIBS, 27 Taylor Street, University of Strathclyde, Glasgow G4 0NR, UK. g.mcconnell@strath.ac.uk
Riis
Erling
E
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003460:Crystallization / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology; D016257:Fura-2 / Q000031:analogs & derivatives*; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D007834:Lasers; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D017785:Photons; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C049925:fura-2-am; D002118:Calcium; D016257:Fura-2