Depolarisation of the plasma membrane in the arsenic trioxide (As2O3)-and anti-CD95-induced apoptosis in myeloid cells.
Depolarisation of the plasma membrane has been shown to be actively regulated during lymphocyte-apoptosis. Here, we present data about anti-Fas and As2O3 induced depolarisation of myeloid U-937 cells. Anti-Fas but not As2O3-induced depolarisation was significantly dependent on caspase-activation. Na+-fluxes contributed to the depolarisation in early stages of As2O3-induced apoptosis, whereas the membrane potential in late stages depended on Cl- -fluxes. Cl- -channels also played an important role in the induction of cell shrinkage in As2O3-induced apoptosis. However, none of these ions contributed significantly to anti-Fas induced depolarisation. This indicates the existence of different mechanisms for apoptotic plasma membrane depolarisation within one cell type.
Nolte
Florian
F
Department of Transfusion Medicine, University of Ulm, Institute for Clinical Transfusion Medicine and Immunogenetics, Ulm, Germany.
Friedrich
Oliver
O
Rojewski
Markus
M
Fink
Rainer H A
RH
Schrezenmeier
Hubert
H
Körper
Sixten
S
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000378:metabolism; D058846:Antibodies, Monoclonal, Murine-Derived; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D022423:Myeloid Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009579:Nitrobenzoates / Q000494:pharmacology; D010087:Oxides / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism; D020298:U937 Cells; D019014:fas Receptor / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D000970:Antineoplastic Agents; D001152:Arsenicals; D018118:Chloride Channels; D002712:Chlorides; D009579:Nitrobenzoates; D010087:Oxides; C411023:anti-Fas monoclonal antibody; D019014:fas Receptor; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D012964:Sodium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D000077237:Arsenic Trioxide