title: Evaluation of four capillary columns for the analysis of organochlorine pesticides, polychlorinated biphenyls, and polybrominated diphenyl ethers in human serum for epidemiologic studies.
abstract: The separation of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ether (PBDE) congeners was evaluated on four capillary columns: 60 m x 0.25 mm i.d., 0.25 microm film thickness RTX-5MS and DB-XLB capillary columns, and 60 m x 0.18 mm i.d., 0.25 microm film thickness DB-XLB and DB-5MS capillary columns. Based on performance, capacity, and cost, the RTX-5MS (60 m x 0.25 mm i.d., 0.25 microm thickness) and the DB-XLB (60 m x 0.25 mm i.d., 0.25 microm film thickness) were selected for the analysis of human serum extracts by using gas chromatography/electron-capture detection. In contrast to previous studies, the oven temperature program affords the separation of congeners that are not separated by using other combinations of capillary columns, most notably PBDE-47 and PCB 180. In addition, the method enables determination of OCPs, PCBs, and PBDEs prevalent in a single extract of serum, which can lead to considerable time savings in the analysis of large number of samples collected for epidemiologic studies.
authors.affiliation: Department of Environmental Toxicology, University of California, Davis, CA 95616, USA. ewrogers@ucdavis.edu
authors.forename: Evan
authors.initials: E
authors.lastname: Rogers
authors.forename: Myrto
authors.initials: M
authors.lastname: Petreas
authors.forename: June-Soo
authors.initials: JS
authors.lastname: Park
authors.forename: Guomao
authors.initials: G
authors.lastname: Zhao
authors.forename: M Judith
authors.initials: MJ
authors.lastname: Charles
mesh_terms: D002849:Chromatography, Gas / Q000295:instrumentation*; D005260:Female; D006801:Humans; D007306:Insecticides / Q000097:blood*; D011075:Polybrominated Biphenyls / Q000097:blood*; D011078:Polychlorinated Biphenyls / Q000097:blood*
chemical_list: D007306:Insecticides; D011075:Polybrominated Biphenyls; D011078:Polychlorinated Biphenyls