title: Experimental analysis of antimicrobial action of dicyclomine hydrochloride.
abstract: Dicyclomine hydrochloride is an antispasmodic agent. The MIC of dicyclomine against standard strains of Gram positive and Gram negative bacteria were performed by NCCLS broth dilution technique. These drugs showed a rapid killing action on Gram positive bacteria, Staphylococcus aureus NCTC 6571, 8530 and several other reference strains. The killing effect against Gram negative bacteria, Shigella boydii 8 NCTC 254/66 and Salmonella typhimurium NCTC 74 showed that the drug was bacteriostatic with respect to these strains. High rate of killing was achieved for most strains of Gram positive bacteria within 2 h. When administered to Swiss strain of white mice at doses of 30 and 60 microg/g of mouse, the drug could significantly protect the animals challenged with 50 MLD of Salmonella typhimurium NCTC 74. According to chi2 test, the in vivo data were highly significant (p<0.001). Since dicyclomine showed a remarkable inhibitory action against several pathogenic bacteria, in the course of time, it may be developed as a potent antimicrobial agent for many bacterial infections.
authors.affiliation: Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
authors.forename: Prithviraj
authors.initials: P
authors.lastname: Karaka
authors.forename: Kuppusamy Asok
authors.initials: KA
authors.lastname: Kumar
authors.forename: Laxmi Rani
authors.initials: LR
authors.lastname: Basu
authors.forename: Asish
authors.initials: A
authors.lastname: Dasgupta
authors.forename: Raja
authors.initials: R
authors.lastname: Ray
authors.forename: Sujata G
authors.initials: SG
authors.lastname: Dastidar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004025:Dicyclomine / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D004025:Dicyclomine