Cyclin E amplification and overexpression in clear cell adenocarcinoma of the ovary.
OBJECTIVE
The purpose of this study is to compare DNA, mRNA and protein levels of the cyclin E between clear cell (CC) and serous (SC) ovarian carcinomas, and evaluate the relationship between cyclin E and p53 status.


METHODS
We examined the DNA, mRNA and protein levels of cyclin E and the protein level of p53 in 44 CCs and 39 SCs using microdissected tissues.


RESULTS
Relative cyclin E mRNA expression was significantly higher in CC (3.62, 95% CI, 2.24-4.99) than in SC (1.75, 95% CI, 1.05-2.45; p = 0.0098). The percentage of positive nuclear staining of cyclin E was significantly higher in CC (48.3, 95% CI, 40.4-56.1) than SC (25.3, 95% CI, 17.4-33.3; p = 0.0001). The mRNA and protein expression of cyclin E was significantly correlated (r = 0.66, p < 0.0001). However, the correlation between relative DNA copy number and relative mRNA expression was not significant (r = -0.063; p = 0.66). Percentage of positive nuclear staining of cyclin E was significantly higher in p53 positive cases (51.8, 95% CI, 40.0-63.5) than p53 negative cases (36.2, 95% CI, 28.2-44.2; p = 0.028).


CONCLUSIONS
Cyclin E expression is significantly higher in CC than in SC. Cyclin E expression is significantly related with p53 positivity.
Tsuda
Hiroshi
H
Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Bandera
Christina A
CA
Birrer
Michael J
MJ
Hashiguchi
Yasunori
Y
Berkowitz
Ross S
RS
Mok
Samuel C
SC
D018262:Adenocarcinoma, Clear Cell / Q000737:chemistry*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D019927:Cyclin E / Q000032:analysis* / Q000235:genetics; D018284:Cystadenocarcinoma, Serous / Q000737:chemistry*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D019927:Cyclin E; D016159:Tumor Suppressor Protein p53