title: [EB virus encoded latent membrane protein 1 mediated expression of transcription factor Ets-1 in nasopharyngeal carcinoma cells].
abstract: OBJECTIVE
  To elucidate the expression of tanscription factor Ets-1 mediated by EB virus encoded latent membrane protein 1 (LMP1) in nasopharyngeal carcinoma (NPC) cells.
  
  
  METHODS
  LMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2) were used. Expression of LMP1 and Ets-1 was observed after induction with Doxycycline (Dox). Expression of Ets-1 mRNA and protein was detected by RT-PCR and Western blot, respectively. The phosphorylation level of Ets-1 protein was examined by co-immunoprecipitation. The DNA binding activity of Ets-1 was detected by electrophoretic-mobility shift assay (EMSA).
  
  
  RESULTS
  After induction with Dox in pTet-on-LMP1 HNE2 cells, to some extent, the expression of Ets-1 mRNA and protein, its phosphorylation level and DNA binding activity were increased with enhancement of LMP1 expression.
  
  
  CONCLUSIONS
  LMP1 induces transcriptional activation and expression of Ets-1 which may contribute to the development of NPC.
authors.affiliation: Department of Pathology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zeng
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Tao
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D004318:Doxycycline / Q000494:pharmacology; D004854:Herpesvirus 4, Human; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D010766:Phosphorylation; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014763:Viral Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D014157:Transcription Factors; D014763:Viral Matrix Proteins; D004318:Doxycycline