Hepatocyte culture in bioartificial livers with different membrane characteristics.
Rat hepatocytes were cultured in three polysulfone, hollow-fiber cartridges, characterized by two membrane variables: pore size and inner diameter (ID). Hepatocytes entrapped in a micro-filtration (MF) cartridge with the membrane pore size 0.1 microm had twice the production of urea and 4-fold the amount of albumin in comparison to the control cartridge, a ultra-filtration (UF) cartridge with a molecular weight cut-off (MWCO) of 100 kDa. Hepatocytes entrapped in a UF cartridge with ID of 0.5 mm secreted twice the amount of urea and 10-fold the amount of albumin compared with the control UF cartridge.
Meng
Qin
Q
Department of Chemical Engineering and Biochemical Engineering, Zhejiang University, 38 Zheda Road, Hangzhou, Zhejiang 310027, PR China. mengq@zju.edu.cn
Zhang
Guoliang
G
Wu
Danqing
D
D000418:Albumins / Q000096:biosynthesis*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D018914:Cells, Immobilized / Q000502:physiology; D022781:Hepatocytes / Q000502:physiology*; D019164:Liver, Artificial; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014508:Urea / Q000378:metabolism*
D000418:Albumins; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D014508:Urea