A study of porcine lymphocyte populations. I. Separation of porcine lymphocyte subpopulations.
Several methods for separating porcine lymphocyte populations were studied. Lymphocyte populations enriched in T cells were obtained by filtration on nylon fiber columns. Lymphocyte populations enriched in B cells were obtained by E rosette sedimentation. Results obtained with anti-immunoglobulin columns were less satisfactory. Attempts to fractionate porcine lymphoid cells with the help of Helix pomatia lectin remained unsuccessful.
Buschmann
H
H
Institut f. Med. Mikrobiologie, Infektions- und Seuchenmedizin, Tierärztliche Fakultät, Universität München, Federal Republic of Germany.
Pawlas
S
S
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D005374:Filtration / Q000662:veterinary; D016131:Lymphocyte Subsets / Q000276:immunology*; D012397:Rosette Formation / Q000662:veterinary; D013552:Swine / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*