Characterization and application of the selective strand annealing activity of the N terminal domain of hepatitis delta antigen.
We used synthetic DNA oligos to investigate the nucleic acid chaperone properties of the N terminal domain of hepatitis delta antigen (NdAg). We found that NdAg possessed a bona fide chaperone activity. NdAg could distinguish subtle differences in the thermal stability of the base pairing region, and enabled DNA oligos to form a more stable duplex among competing sequences through facilitating strand annealing selectively, stimulating duplex conversion selectively, and stabilizing the more stable duplex. The property of NdAg identified in this study could be applied to improve the efficiency and specificity of dot blot hybridization under conditions of low stringency.
Huang
Zhi-Shun
ZS
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan, ROC.
Chen
Albert You-Ju
AY
Wu
Huey-Nan
HN
D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000378:metabolism*; D035923:Hepatitis delta Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D017403:In Situ Hybridization; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
D035923:Hepatitis delta Antigens; D018832:Molecular Chaperones