Technical aspects of an impact acceleration traumatic brain injury rat model with potential suitability for both microdialysis and PtiO2 monitoring.
This report describes technical adaptations of a traumatic brain injury (TBI) model-largely inspired by Marmarou-in order to monitor microdialysis data and PtiO2 (brain tissue oxygen) before, during and after injury. We particularly focalize on our model requirements which allows us to re-create some drastic pathological characteristics experienced by severely head-injured patients: impact on a closed skull, no ventilation immediately after impact, presence of diffuse axonal injuries and secondary brain insults from systemic origin... We notably give priority to minimize anaesthesia duration in order to tend to banish any neuroprotection. Our new model will henceforth allow a better understanding of neurochemical and biochemical alterations resulting from traumatic brain injury, using microdialysis and PtiO2 techniques already monitored in our Intensive Care Unit. Studies on efficiency and therapeutic window of neuroprotective pharmacological molecules are now conceivable to ameliorate severe head-injury treatment.
Carré
Emilie
E
Department of Neurochemistry, I.M.N.S.S.A., B.P. 610, 83800 Toulon Armées, France. emilie_carre@hotmail.com
Cantais
Emmanuel
E
Darbin
Olivier
O
Terrier
Jean-Pierre
JP
Lonjon
Michel
M
Palmier
Bruno
B
Risso
Jean-Jacques
JJ
D000054:Acceleration / Q000009:adverse effects*; D018681:Anesthetics, General / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology; D001930:Brain Injuries / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D020833:Diffuse Axonal Injury / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D045604:Extracellular Fluid / Q000378:metabolism; D016489:Head Injuries, Closed / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D017551:Microdialysis; D009410:Nerve Degeneration / Q000473:pathology / Q000503:physiopathology; D018696:Neuroprotective Agents / Q000494:pharmacology; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D018681:Anesthetics, General; D018696:Neuroprotective Agents