Study on the interactions between anti-HIV-1 active compounds with trans-activation response RNA by affinity capillary electrophoresis.
The study on the interactions between two anti-human immunodeficiency virus type 1 (anti-HIV-1) active compounds with trans-activation response (TAR) RNA by affinity capillary electrophoresis (ACE) with UV absorbance detection is presented. The results showed that the novel active molecules could interact with TAR RNA and inhibit the reproduce process of HIV-1. The binding constants were estimated by the change of migration time of the analytes through the change of concentrations of TAR RNA in the buffer solution. The yielded binding constants of 8.87 x 10(3)M(-1) for active compound C(3) and 8.42 x 10(3)M(-1) for MC(3) at 20.0 degrees C, 0.626 x 10(3)M(-1) and 0.644 x 10(3)M(-1) at 37.0 degrees C, respectively. The thermodynamic parameters Delta H and DeltaS were obtained and shown that both hydrophobic and electrostatic interaction played roles in the binding processes. The results showed that the presented method was an easy and simple method to evaluate the interaction of small molecules with some bioactive materials.
Ding
Li
L
College of Chemistry, Peking University, Beijing 100871, China.
Zhang
Xin-Xiang
XX
Chang
Wen-Bao
WB
Lin
Wei
W
Yang
Ming
M
D019380:Anti-HIV Agents / Q000494:pharmacology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D012313:RNA / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D019380:Anti-HIV Agents; D012313:RNA