Identification of gene expression modifications in myostatin-stimulated myoblasts.
Myostatin belongs to the transforming growth factor beta superfamily and has been shown to function as an inhibitor of skeletal muscle proliferation and differentiation. To gain insight into the molecular mechanisms of myostatin function during myogenesis, differential display reverse transcription PCR was employed to identify altered gene expressions associated with myostatin inhibitory function in chicken fetal myoblasts (CFMs). In this work, we have identified seven up-regulated and 12 down-regulated genes in myostatin stimulated CFMs. Those genes are involved in myogenic differentiation, cell architecture, energy metabolism, signal transduction, and apoptosis. The down-regulation of muscle creatine kinase B, troponin C, and myosin regulatory light chain is in agreement with the myostatin negative role in myocyte differentiation. In addition, the expression alteration of skeletal muscle-specific cardiac ankyrin repeat protein and the bcl-2 related anti-apoptotic protein Nr-13 suggests possible unique roles for myostatin in regulating myogenesis by controlling cofactors participated transcriptional regulation and apoptosis.
Yang
Wei
W
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, PR China.
Zhang
Yong
Y
Ma
Guoda
G
Zhao
Xinyi
X
Chen
Yan
Y
Zhu
Dahai
D
D000818:Animals; D030161:Avian Proteins / Q000378:metabolism; D002642:Chick Embryo; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism*; D055435:Myostatin; D009928:Organ Specificity; D010369:Pectoralis Muscles / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D030161:Avian Proteins; D008565:Membrane Proteins; D055435:Myostatin; C093264:NR13 protein, avian; D016212:Transforming Growth Factor beta