title: Host response after reconstruction of abdominal wall defects with porcine dermal collagen in a rat model.
abstract: OBJECTIVE
  The purpose of this study was to compare the inflammatory response after implantation of Pelvicol with Prolene in a rat model.
  
  
  METHODS
  Full-thickness abdominal wall defects were created in 64 Wistar rats, and reconstructed with either Pelvicol or Prolene. Animals were sacrificed on days 7, 14, 30, and 90 to evaluate the presence of herniation, infection, adhesions, and changes in thickness and tensile strength of the implants. Histopathology and immunohistochemistry were performed to evaluate the collagen deposition and the inflammatory response. Statistics were done with unpaired t test and Mann-Whitney rank test.
  
  
  RESULTS
  Pelvicol implantation induced infiltration of granulocytes, macrophages, and NK cells, which showed up-regulated expression of surface activation markers ICAM-1 and CD11b. This inflammatory response was significantly milder, and declined faster than in Prolene-implanted rats, and was also associated with fewer adhesions. Moreover, Pelvicol induced a slower, but more orderly collagen deposition, paralleling the surface of the implant. Pelvicol implants showed a slower increase in thickness and tensile strength early on, but this difference disappeared by day 90.
  
  
  CONCLUSIONS
  Pelvicol induces a milder inflammatory response, less adhesion formation, more orderly collagen deposition than Prolene, and reaches a comparable tensile strength only after 90 days.
authors.affiliation: Center for Surgical Technologies, Faculty of Medicine, University Hospitals Leuven, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Eric
authors.initials: E
authors.lastname: Verbeken
authors.forename: Filip
authors.initials: F
authors.lastname: Claerhout
authors.forename: Maxime
authors.initials: M
authors.lastname: Fastrez
authors.forename: Dirk
authors.initials: D
authors.lastname: De Ridder
authors.forename: Jan
authors.initials: J
authors.lastname: Deprest
mesh_terms: D034861:Abdominal Wall / Q000473:pathology* / Q000601:surgery; D000818:Animals; D001707:Biopsy, Needle; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005549:Foreign-Body Reaction / Q000453:epidemiology / Q000473:pathology*; D006084:Graft Rejection; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008422:Materials Testing; D011126:Polypropylenes; D011138:Polytetrafluoroethylene; D019736:Prostheses and Implants / Q000009:adverse effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D019651:Plastic Surgery Procedures / Q000009:adverse effects* / Q000379:methods; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013526:Surgical Mesh; D013552:Swine; D013718:Tensile Strength; D014596:Uterine Prolapse / Q000601:surgery
chemical_list: D011126:Polypropylenes; D011138:Polytetrafluoroethylene; D003094:Collagen