Identification of selenomethionine in selenized yeast using two-dimensional liquid chromatography-mass spectrometry based proteomic analysis.
Selenium-enriched yeast has been commonly used as a nutritional supplement. Here we describe a protocol used to investigate the metabolic fate of inorganic selenium in yeast. We provide definitive, mass spectrometry based evidence for the non-specific incorporation of selenomethionine in the yeast proteome involving the replacement of about 30% of all methionine with selenomethionine.
McSheehy
S
S
Chemical Metrology, Institute for National Measurement Standard, National Research Council, Ottawa, Ontario, Canada K1L 0R6.
Kelly
J
J
Tessier
L
L
Mester
Z
Z
D019587:Dietary Supplements; D008401:Gas Chromatography-Mass Spectrometry; D007091:Image Processing, Computer-Assisted; D008715:Methionine / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D012645:Selenomethionine / Q000032:analysis* / Q000378:metabolism; D015002:Yeast, Dried / Q000737:chemistry* / Q000378:metabolism
D012645:Selenomethionine; D008715:Methionine