Kinetic of improved production and carboxymethyl cellulose hydrolysis by an endo-glucanase from a derepressed mutant of Cellulomonas biazotea.
The maximum product yield of endo-glucanase (650 IU g(-1) substrate) from Cellulomonas biazotea mutant 51 Sm(r) was 1.5- to 2.5-fold more than was produced by the wild type cells and was twice that reported by previous researchers. Mutation substantially improved the enthalpy (DeltaH (*)) and entropy of activation (DeltaS (*)) for product formation, turnover number, specificity constant activation energy, free energies for transition state formation and substrate binding for CMC hydrolysis respectively.
Rajoka
M I
MI
National Institute for Biotechnology and Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan. mirajoka@nibge.org
Ashraf
Yasmin
Y
Khalid
A M
AM
D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics; D040143:Cellulomonas / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D007700:Kinetics; D009154:Mutation
D002480:Cellulase; D002266:Carboxymethylcellulose Sodium