title: Promoter-probe cassettes with the gusA (beta-glucuronidase) reporter gene and several different antibiotic resistance markers.
abstract: A series of gene cassettes with promoterless gusA reporter genes encoding beta-glucuronidase (GUS), linked to different antibiotic resistance markers, have been developed. The gusA-antibiotic resistance gene cassettes can be used to generate transcriptional fusions useful for expression studies.
authors.affiliation: Department of Biology, 1001 E. 3rd St., Jordan Hall 142, Indiana University, Bloomington, IN 47405-1847, United States.
authors.forename: Xuesong
authors.initials: X
authors.lastname: He
authors.forename: Manika
authors.initials: M
authors.lastname: Bhateja
authors.forename: Clay
authors.initials: C
authors.lastname: Fuqua
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism*; D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015415:Biomarkers; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D015415:Biomarkers; D004269:DNA, Bacterial; C088386:homoserine lactone; D005966:Glucuronidase; D015107:4-Butyrolactone