Isolation of mini-Tn5Km2 insertion mutants of Salmonella enterica serovar Oranienburg sensitive to NaCl-induced osmotic stress.
We previously reported that the viability of Salmonella Oranienburg strains under NaCl stress was variable and depended on the strain's origin; food strains were resistant and patient strains sensitive to NaCl. Therefore, we mutagenized a food strain with a mini-Tn5Km2 transposon. Of 2,400 mutants screened, 15 NaCl-sensitive mutants were isolated, and 7 genes associated with NaCl-sensitivity were identified. The intact genes complemented their own food-strain mutants, but not patient-strain mutants, suggesting that the difference in NaCl-sensitivity between food and patient S. Oranienburg strains might not arise from a single gene mutation, but from change in multiple osmoregulatory mechanisms in Salmonella.
Asakura
Hiroshi
H
Laboratory of Food Microbiology, Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. hasakura@nihs.go.jp
Panutdaporn
Nantika
N
Kawamoto
Keiko
K
Igimi
Shizunobu
S
Yamamoto
Shigeki
S
Makino
Sou-ichi
S
D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005516:Food Microbiology; D005816:Genetic Complementation Test; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009997:Osmotic Pressure; D016133:Polymerase Chain Reaction; D012478:Salmonella Food Poisoning / Q000382:microbiology*; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012965:Sodium Chloride