title: Visualization and examination of the meiotic spindle in human oocytes with polscope.
abstract: OBJECTIVE
  The effect of detectability/location of meiotic spindle to fertilization of human MII oocytes and outcome of clinical IVF/ICSI-ET cycles was studied.
  
  
  METHODS
  The location of spindle relative to polar body positioned to 6 o'clock was detected with polscope prior to ICSI in oocytes. Data of 83 IVF/ICSI-ET cycle, including pregnancy results, and a total of 1033 oocytes examined were analyzed retrospectively.
  
  
  RESULTS
  Polar body position does not always accurately predict spindle position. Similar fertilization was observed in the polscope detected and in the control group. The main number of oocytes and spindle positive oocytes collected was higher in the pregnant than in the non-pregnant group. Higher fertilization was found in that group where 2/3-d of the oocytes had detected spindle. The main number of oocytes and spindle positive oocytes obtained decreased with increasing age of patients.
  
  
  CONCLUSIONS
  The presence of birefringent spindle predicts higher fertilization and development and greater probability of pregnancy.
authors.affiliation: Infertility and IVF Center of Buda, Saint János Hospital, Budapest, Hungary.
authors.forename: Janos
authors.initials: J
authors.lastname: Konc
authors.forename: Katalin
authors.initials: K
authors.lastname: Kanyó
authors.forename: Sandor
authors.initials: S
authors.lastname: Cseh
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019723:Endoscopes; D005260:Female; D005306:Fertilization; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D011256:Pregnancy Outcome; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus / Q000648:ultrastructure*