title: Multiple replication origins within the inverted repeat region of the Plasmodium falciparum apicoplast genome are differentially activated.
abstract: The 35 kb circular genome (plDNA) of the Plasmodium falciparum apicoplast replicates by the bidirectional ori/D-loop mechanism. PlDNA replication was previously shown to initiate within the inverted repeat (IR) region of the apicoplast genome [Williamson DH, Preiser PR, Moore PW, McCready S, Strath M, Wilson RJM (Iain). The plastid DNA of the malaria parasite Plasmodium falciparum is replicated by two mechanisms. Mol Microbiol 2002;45:533-42; Singh D, Chaubey S, Habib S. Replication of the Plasmodium falciparum apicoplast DNA initiates within the inverted repeat region. Mol Biochem Parasitol 2003;126:9-14.] and the presence of at least two ori within each segment of the IR was postulated. Using 5' end-labelled nascent DNA as probe, we now demonstrate the utilization of several putative ori located within the IR for plDNA replication. Quantitation of signals obtained for different segments of the IR as well as determination of the number of molecules emanating from two ori regions by competitive PCR analysis indicated differential strengths of ori during plDNA replication prior to schizogony.
authors.affiliation: Division of Molecular and Structural Biology, Central Drug Research Institute, P.O. Box 173, Chattar Manzil, Mahatma Gandhi Marg, Lucknow 226001, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Singh
authors.forename: Ambrish
authors.initials: A
authors.lastname: Kumar
authors.forename: E V S
authors.initials: EV
authors.lastname: Raghu Ram
authors.forename: Saman
authors.initials: S
authors.lastname: Habib
mesh_terms: D000818:Animals; D004261:DNA Replication / Q000502:physiology*; D016054:DNA, Protozoan / Q000096:biosynthesis*; D018503:Genome, Protozoan; D015388:Organelles / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology*; D018741:Replication Origin
chemical_list: D016054:DNA, Protozoan