title: Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.
abstract: The core structure of the lipopolysaccharide (LPS) isolated from a rough strain of the phytopathogenic bacterium Pseudomonas syringae pv. phaseolicola, GSPB 711, was investigated by sugar and methylation analyses, Fourier transform ion-cyclotron resonance ESI MS, and one- and two-dimensional 1H-, 13C- and 31P-NMR spectroscopy. Strong alkaline deacylation of the LPS resulted in two core-lipid A backbone undecasaccharide pentakisphosphates in the ratio approximately 2.5 : 1, which corresponded to outer core glycoforms 1 and 2 terminated with either L-rhamnose or 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), respectively. Mild acid degradation of the LPS gave the major glycoform 1 core octasaccharide and a minor truncated glycoform 2 core heptasaccharide, which resulted from the cleavage of the terminal Kdo residues. The inner core of P. syringae is distinguished by a high degree of phosphorylation of L-glycero-D-manno-heptose residues with phosphate, diphosphate and ethanolamine diphosphate groups. The glycoform 1 core is structurally similar but not identical to one of the core glycoforms of the human pathogenic bacterium Pseudomonas aeruginosa. The outer core composition and structure may be useful as a chemotaxonomic marker for the P. syringae group of bacteria, whereas a more conserved inner core structure appears to be representative for the whole genus Pseudomonas.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences Moscow, Russia. evelina@ioc.ac.ru
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Galina M
authors.initials: GM
authors.lastname: Zdorovenko
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Olga V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Klaus
authors.initials: K
authors.lastname: Rudolph
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zähringer
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D044224:Pseudomonas syringae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008070:Lipopolysaccharides