title: Cy3B: improving the performance of cyanine dyes.
abstract: The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an antibody. This study compared the conjugated emission properties of Cy3B with other commercially available fluorophores emitting at similar wavelengths.
authors.affiliation: Amersham Biosciences, Discovery Systems, The Maynard Centre, Whitchurch, Cardiff, CF14 7YT, United Kingdom. michael.cooper@amersham.com
authors.forename: Michael
authors.initials: M
authors.lastname: Cooper
authors.forename: Andreas
authors.initials: A
authors.lastname: Ebner
authors.forename: Mark
authors.initials: M
authors.lastname: Briggs
authors.forename: Miles
authors.initials: M
authors.lastname: Burrows
authors.forename: Nicholas
authors.initials: N
authors.lastname: Gardner
authors.forename: Robert
authors.initials: R
authors.lastname: Richardson
authors.forename: Richard
authors.initials: R
authors.lastname: West
mesh_terms: D001557:Benzenesulfonates / Q000138:chemical synthesis / Q000737:chemistry*; D002232:Carbocyanines / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry
chemical_list: D001557:Benzenesulfonates; D002232:Carbocyanines; C495965:Cy3B N-hydroxysuccinimide ester; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; C089187:cyanine dye 3