title: Characterization of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis in Brazil.
abstract: In this study the nucleotide sequence of the pncA gene from 59 Mycobacterium tuberculosis clinical isolates was analyzed. Mutations in the pncA gene were identified in 29 of 40 pyrazinamide-resistant isolates, and no pyrazinamidase activity was detected in 39 of them. Twelve mutations found in this work have not been described previously.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Rua Bento Gonçalves 9500, Prédio 43421, CEP 91501-970 Porto Alegre, RS, Brazil.
authors.forename: Vívian de F Sumnienski
authors.initials: Vde F
authors.lastname: Rodrigues
authors.forename: Maria Alice
authors.initials: MA
authors.lastname: Telles
authors.forename: Marta Osório
authors.initials: MO
authors.lastname: Ribeiro
authors.forename: Patrícia Izquierdo
authors.initials: PI
authors.lastname: Cafrune
authors.forename: Maria Lucia Rosa
authors.initials: ML
authors.lastname: Rossetti
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Zaha
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D011718:Pyrazinamide / Q000494:pharmacology*; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis