Phage display vectors for the in vitro generation of human antibody fragments.
A major source of human antibodies are phage display libraries, which are constructed from various genetic sources. Antibodies are expressed as scFV and Fab antibody fragments using various vector systems. This review offers a comprehensive overview of M13 phage display antibody vectors and discusses their applications.
Hust
Michael
M
Department of Biotechnology, Institute of Biochemistry and Biotechnology, Carolo-Wilhelmina Technical University, Braunschweig, Germany.
Dübel
Stefan
S
D000911:Antibodies, Monoclonal / Q000235:genetics*; D017104:Bacteriophage M13 / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*
D000911:Antibodies, Monoclonal; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region