title: Hepatic triglyceride content and its relation to body adiposity: a magnetic resonance imaging and proton magnetic resonance spectroscopy study.
abstract: BACKGROUND
  Hepatic steatosis is associated with obesity and type II diabetes. Proton magnetic resonance spectroscopy (1H MRS) is a non-invasive method for measurement of tissue fat content, including intrahepatocellular lipids (IHCL) and intramyocellular lipids (IMCL).
  
  
  METHODS
  We used 1H MRS and whole body magnetic resonance imaging (MRI) to assess the relationship between IHCL accumulation, total body adipose tissue (AT) content/distribution, and IMCL content in 11 subjects with biopsy proven hepatic steatosis and 23 normal volunteers.
  
  
  RESULTS
  IHCL signals were detectable in all subjects but were significantly greater in hepatic steatosis (geometric mean (GM) 11.5 (interquartile range (IQR) 7.0-39.0)) than in normal volunteers (GM 2.7 (IQR 0.7-9.3); p=0.02). In the study group as a whole, IHCL levels were significantly greater in overweight compared with lean subjects (body mass index (BMI) >25 kg/m2 (n=23): GM 7.7 (IQR 4.0-28.6) v BMI <25 kg/m2 (n=11): GM 1.3 (IQR 0.3-3.6; p=0.004)). There was a significant association between IHCL content and indices of overall obesity (expressed as a percentage of body weight) for total body fat (p=0.001), total subcutaneous AT (p=0.007), and central obesity (subcutaneous abdominal AT (p=0.001) and intra-abdominal AT (p=0.001)), after allowing for sex and age. No correlation between IHCL content and IMCL was observed. A significant correlation was observed between serum alanine aminotransferase and liver fat content (r=0.57, p=0.006).
  
  
  CONCLUSIONS
  Our results suggest that hepatic steatosis appears to be closely related to body adiposity, especially central obesity. MRS may be a useful method for monitoring IHCL in future interventional studies.
authors.affiliation: Robert Steiner MR Unit, Imaging Sciences Department, MRC Clinical Sciences Centre, Hammersmith Hospital, Du Cane Rd, London W12 0HS, UK.
authors.forename: E L
authors.initials: EL
authors.lastname: Thomas
authors.forename: G
authors.initials: G
authors.lastname: Hamilton
authors.forename: N
authors.initials: N
authors.lastname: Patel
authors.forename: R
authors.initials: R
authors.lastname: O'Dwyer
authors.forename: C J
authors.initials: CJ
authors.lastname: Doré
authors.forename: R D
authors.initials: RD
authors.lastname: Goldin
authors.forename: J D
authors.initials: JD
authors.lastname: Bell
authors.forename: S D
authors.initials: SD
authors.lastname: Taylor-Robinson
mesh_terms: D000005:Abdomen / Q000473:pathology; D000273:Adipose Tissue / Q000473:pathology; D000328:Adult; D000368:Aged; D000886:Anthropometry; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000737:chemistry* / Q000473:pathology; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000737:chemistry; D009765:Obesity / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D014280:Triglycerides / Q000032:analysis*
chemical_list: D008055:Lipids; D014280:Triglycerides