Natural polyelectrolyte films based on layer-by layer deposition of collagen and hyaluronic acid.
The aim of the present work was to assemble extracellular matrix components into polyelectrolyte multilayers using the layer-by-layer deposition method. The films are constructed with type-I collagen and hyaluronic acid. The construction exhibits the general features observed during polyelectrolyte multilayer buildup: alternate positive and negative values of the zeta potential of the film during its construction and regular increase of the film thickness with the number, n, of deposition step. This increase is shown to be linear with n. As expected for a linearly growing film, the confocal microscopy shows that when the film is brought in contact with a collagen solution, collagen does not diffuse into the film but interacts only with its outer layer. However, the films are not constituted of homogeneously distributed polyanion/polycation complexes as it is usually observed, but they are formed of fibers as imaged by AFM. The typical width of these fibers increases with the number of deposition steps. Finally, it is found that chondrosarcoma cells spread well and synthesize extracellular matrix components only on the collagen ending films, whereas no cellular matrix was found for HA ending ones. Such architectures may be further functionalized by inclusion of active drugs, peptides, proteins..., and could be used as tunable biomaterial interfaces.
Zhang
Jun
J
Institut National de la Santé et de la Recherche Scientifique, Unité 595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, 11 rue Humann, 67085 Strasbourg Cedex, France.
Senger
Bernard
B
Vautier
Dominique
D
Picart
Catherine
C
Schaaf
Pierre
P
Voegel
Jean-Claude
JC
Lavalle
Philippe
P
D001672:Biocompatible Materials / Q000737:chemistry*; D032701:Biomimetics; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002813:Chondrosarcoma / Q000378:metabolism; D003094:Collagen / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
D001672:Biocompatible Materials; D003094:Collagen; D004573:Electrolytes; D006820:Hyaluronic Acid; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers