title: Proton exchange in type II isopentenyl diphosphate isomerase.
abstract: [reaction: see text] Type II isopentenyl diphosphate:dimethylallyl diphosphate (IPP:DMAPP) isomerase from Synechocystis PCC 6803 catalyzes the interconversion of IPP and DMAPP. Upon incubation of the enzyme with IPP or DMAPP in 2H2O, one deuterium is incorporated into the C2 methylene of IPP, two deuteriums are incorporated at C4, and three deuteriums are incorporated into the (E)-methyl of DMAPP.
authors.affiliation: Department of Chemistry, University of Utah, 315 South 1400 East, RM 2020, Salt Lake City, Utah 84112-0850, USA.
authors.forename: Sam J
authors.initials: SJ
authors.lastname: Barkley
authors.forename: Shrivallabh B
authors.initials: SB
authors.lastname: Desai
authors.forename: C Dale
authors.initials: CD
authors.lastname: Poulter
mesh_terms: D019748:Carbon-Carbon Double Bond Isomerases / Q000737:chemistry*; D002384:Catalysis; D004789:Enzyme Activation; D045782:Hemiterpenes / Q000138:chemical synthesis / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D011522:Protons; D013237:Stereoisomerism; D046939:Synechocystis / Q000145:classification / Q000201:enzymology*
chemical_list: D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011522:Protons; C004809:isopentenyl pyrophosphate; C043060:3,3-dimethylallyl pyrophosphate; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase