Monitoring the formation of biosilica catalysed by histidine-tagged silicatein.
Surface bound silicatein retains its biocatalytic activity, which was demonstrated by monitoring the immobilisation of silicatein using a histidine-tag chelating anchor and the subsequent biosilicification of SiO(2) on surfaces by surface plasmon resonance spectroscopy, atomic force microscopy and scanning electron microscopy.
Tahir
Muhammad Nawaz
MN
Institut für Anorganische Chemie, Universität Mainz, Duesbergweg 10-14, D-55128 Mainz, Germany.
Théato
Patrick
P
Müller
Werner E G
WE
Schröder
Heinz C
HC
Janshoff
Andreas
A
Zhang
Jian
J
Huth
Joachim
J
Tremel
Wolfgang
W
D002384:Catalysis; D002403:Cathepsins / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism*; D020349:Surface Plasmon Resonance
D006639:Histidine; D012822:Silicon Dioxide; D002403:Cathepsins