Olfactory transduction in ciliated receptor neurons of the Cabinza grunt, Isacia conceptionis (Teleostei: Haemulidae).
The ciliated receptor neurons of fish olfactory organs are thought to transduce amino acids through a cAMP-dependent transduction pathway, but direct physiological evidence for this hypothesis remains scarce and is confined to catfish and trout. We investigated olfactory transduction in a marine fish, the Cabinza grunt Isacia conceptionis (Perciformes, Teleostei). The olfactory epithelium was characterized using light and electron microscopy, and isolated ciliated receptor neurons were recorded with the perforated patch-clamp technique. Cells were stimulated with puffer pipettes containing amino acid odourants, IBMX plus forskolin or 8 bromo-cAMP. All three stimuli triggered transient inward currents at a holding potential of -70 mV and responses with outward-rectifying current-voltage relationships. The characteristics of the transduction currents induced by each stimulus were similar across cells and indistinguishable within the same cell, supporting the hypothesis of a cAMP pathway mediating transduction of amino acids in ciliated olfactory receptor neurons.
Schmachtenberg
Oliver
O
Centro de Neurociencias de Valparaiso, Facultad de Ciencias, Universidad de Valparaiso, Avda. Gran Bretania 1111, Playa Ancha, Valparaiso, Chile. oliver@cnv.cl
Bacigalupo
Juan
J
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002923:Cilia / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D066298:In Vitro Techniques; D009831:Olfactory Mucosa / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010473:Perciformes / Q000033:anatomy & histology / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
D000596:Amino Acids; D015056:1-Methyl-3-isobutylxanthine