Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins.
Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.
Amoroso
M G
MG
School of Biotechnological Sciences, University of Naples Federico II, Via Università 133, Portici, Naples, 80055, Italy.
Longobardo
L
L
Capparelli
R
R
D005434:Flow Cytometry / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D006244:Hardness; D006245:Hardness Tests / Q000379:methods*; D010940:Plant Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000502:physiology*; D013223:Statistics as Topic; D014908:Triticum / Q000502:physiology*
D010940:Plant Proteins; C082942:puroindoline protein, Triticum aestivum