title: Conspecificity of the cerulenin and helvolic acid producing 'Cephalosporium caerulens', and the hypocrealean fungus Sarocladium oryzae.
abstract: Fermentation processes for the biochemical reagents cerulenin and helvolic acid employ 'Cephalosporium caerulens,' an invalidly published designation that has been used for more than 40 years. However, its identity has never been critically examined because strains were unavailable from major culture collections. An authentic strain of C. caerulens', derived from the original strain KF-140, was recently found and compared to Sarocladium oryzae, another Acremonium-like fungus which also produces cerulenin and helvolic acid. Morphological comparisons, rDNA sequence data, and chromatography of secondary metabolites established that 'C. caerulens' and S. oryzae are conspecific. Sequence data from ribosomal DNA genes indicated S. oryzae belongs to the Hypocreales and is allied with members of the Ceratostomataceae, Scopinella species, Emericellopsis species and certain Acremonium-like anamorphs of uncertain familial relationships. At least two of the isolates of S. oryzae produced titres of cerulenin and helvolic acid similar to those of KF-140. This finding demonstrates that manufacture of cerulenin need not be limited to the original strain.
authors.affiliation: Centro de Investigación Básica, Merck Sharp and Dohme de España, Josefa Valcárcel 38, ES-28027 Madrid, Spain. gerald_bills@merck.com
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Bills
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Platas
authors.forename: Walter
authors.initials: W
authors.lastname: Gams
mesh_terms: D000164:Acremonium / Q000378:metabolism*; D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002569:Cerulenin / Q000096:biosynthesis / Q000378:metabolism*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal; D005672:Fusidic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D002569:Cerulenin; D005672:Fusidic Acid; C011267:helvolic acid