Isolations of salicylic acid-induction-expressed genes in chilling-stressed banana seedling leaves using mRNA differential display.
The mRNA differential display method was applied to identify banana genes that were regulated by salicylic acid (SA) during chilling stress. Eighteen cDNA fragments induced by SA during chilling stress were retracted. Seven of them were affirmed by reverse Northern hybridization to be significantly induced. Two most differential fragments (G and A) of them were cloned and sequenced. Nucleotide sequence analysis showed that clone G fragment had 92% homology to partial cDNA sequences of two cold-related genes in soybean (Glycine max) and clone A did not show any identity to previously reported sequences in GenBank database.
Kang
Guo-Zhang
GZ
National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002, China.
Zhu
Guo-Hui
GH
Peng
Xin-Xiang
XX
Sun
Gu-Chou
GC
Wang
Zheng-Xun
ZX
D001483:Base Sequence; D015152:Blotting, Northern; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D028521:Musa / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology*; D036226:Seedlings / Q000235:genetics*
D018076:DNA, Complementary; D020156:Salicylic Acid