A robust approach to studying the adsorption of Pluronic F108 on nonporous membranes.
A method for poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) desorption from synthetic nonporous polymeric membranes, using hexane:isopropanol treatment and subsequent colorimetric quantification, is described. The polymers polysulfone, poly(vinyldiene fluoride), and poly(ether imide) were used to fabricate solid adsorption matrices. The desorbed Pluronic F108 forms a color complex with ammonium ferrothiocyanate (NH4FeSCN) and is based on partitioning of a chromophore present in NH4FeSCN from an aqueous phase to a chloroform phase in the presence of Pluronic. The protocols for Pluronic desorption and detection are simple, sensitive, inexpensive, rapid, and reproducible over a wide range of Pluronic coating concentrations and membrane surface chemistries. A linear response over the concentration range from 3 to 130 microg ml(-1) is obtained. The adsorption isotherms for flat sheet membranes are also described and the Langmuir equation provides the best fit for the adsorption data obtained within the concentration range studied. The absence of any significant interference from certain proteins, vitamins, carbohydrates, plasma, and halogenated derivatives makes the assay equally suitable for the estimation of Pluronic F108 in the attendant Pluronic conjugates or in biomedical applications. Using nonporous hollow fine fibers and capillary membranes as model curved substrates we were also able to correlate an increase in the radius of curvature with a corresponding increase in the surface interfacial adsorption of Pluronic F108.
Govender
S
S
Department of Biochemistry, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
Jacobs
E P
EP
Bredenkamp
M W
MW
Swart
P
P
D000327:Adsorption; D001672:Biocompatible Materials; D001703:Biophysics / Q000379:methods*; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D006586:Hexanes / Q000737:chemistry; D007454:Iodides / Q000737:chemistry; D046911:Macromolecular Substances; D008566:Membranes; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D010825:Physics / Q000379:methods; D020442:Poloxamer / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
D001672:Biocompatible Materials; D006586:Hexanes; D007454:Iodides; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D013501:Surface-Active Agents; D020442:Poloxamer