title: Determination of oligoelements in parenteral formulations by planar chromatography and spectrophotometry.
abstract: The authors have studied the chromatographic behavior of parenteral preparations for pediatric use containing inorganic cations. After separation and identification by thin-layer chromatography, Mn(2+), Zn(2+), and Cu(2+) were analyzed by a method based on reaction with an appropriate reagent and extraction with an organic solvent which yielded elution and preconcentration, resulting in an appropriate solution for colorimetric quantitation. Cr(3+) cation was determined by atomic absorption spectrophotometry after appropriate chromatographic separation, using microcrystalline cellulose (adsorbent) and an acetone:water:hydrochloric acid mixture (80:5:8) as the mobile phase.
authors.affiliation: Department of Pharmaceuticals, Faculty of Pharmaceutical Sciences, UNESP, 14801-902, Araraquara, São Paulo, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Mancini
authors.forename: J
authors.initials: J
authors.lastname: Zuanon Netto
mesh_terms: D002626:Chemistry, Pharmaceutical; D002675:Child, Preschool; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003300:Copper / Q000032:analysis*; D006801:Humans; D007223:Infant; D008345:Manganese / Q000032:analysis*; D010288:Parenteral Nutrition; D010372:Pediatrics; D015032:Zinc / Q000032:analysis*
chemical_list: D008345:Manganese; D003300:Copper; D015032:Zinc