title: A mouse model of cerebral oligemia: relation to brain histopathology, cerebral blood flow, and energy state.
abstract: An animal model involving stepwise occlusion of the common carotid arteries (sCCAO) in DBA/2 mice is presented in which the right and left carotid arteries were permanently ligated within a time interval of four weeks. Thereafter, cerebral functional and structural parameters were determined at acute (15 min) and subchronic (1 day; 3, 7, and 14 days) time points after sCCAO. Quantitative changes in regional cerebral blood flow (rCBF) as determined by the [14C]iodoantipyrine method, energy state (ATP, phosphocreatine, ADP, AMP, adenosine) as shown by HPLC, brain histopathology, and neuronal densities were measured in both hemispheres. Acute sCCAO was accompanied by a drastic reduction in cerebral energy-rich phosphate concentrations, ATP and phosphocreatine, and in rCBF of more than 50%. In contrast, cortical adenosine increased around five-fold. Subchronic sCCAO, however, was associated with normalization in brain energy metabolites and near-complete restoration of rCBF, except in the caudate nucleus (-40%). No marked signs of necrotic or apoptotic cell destruction were detected. Thus, during the subchronic period, compensatory mechanisms are induced to counteract the drastic changes seen after acute vessel occlusion. In conclusion, this sCCAO mouse model may be useful for long-lasting investigations of stepwise deterioration contributing to chronic cerebrovascular disorders.
authors.affiliation: Clinic of Anesthesiology, University of Heidelberg, Im Neuenheimer Feld 110, 69120, Heidelberg, Germany. konstanze_plaschke@med.uni-heidelberg.de
authors.forename: Konstanze
authors.initials: K
authors.lastname: Plaschke
authors.forename: Clemens
authors.initials: C
authors.lastname: Sommer
authors.forename: Helmut
authors.initials: H
authors.lastname: Schroeck
authors.forename: Dejana
authors.initials: D
authors.lastname: Matejic
authors.forename: Marika
authors.initials: M
authors.lastname: Kiessling
authors.forename: Eike
authors.initials: E
authors.lastname: Martin
authors.forename: Markus A
authors.initials: MA
authors.lastname: Weigand
authors.forename: Hubert J
authors.initials: HJ
authors.lastname: Bardenheuer
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D016893:Carotid Stenosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D051379:Mice; D008811:Mice, Inbred DBA; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010085:Oxidative Phosphorylation; D010725:Phosphocreatine / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
chemical_list: D015415:Biomarkers; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D000241:Adenosine