Cloning and characterization of the tomato karyopherin alpha1 gene promoter.
The karyopherin alpha1 (LeKAPalpha 1) gene of tomato (Lycopersicon esculentum) encodes a receptor involved in nuclear import. To analyze the expression pattern of this gene, a genomic clone containing its upstream region was isolated and sequenced. To study the promoter functionality, a 2170 bp fragment (LM1), was fused to glucuronidase (GUS) and introduced into petunia cells by particle bombardment. For further characterization of the promoter, one inverse and three deletion constructs were studied in cell suspension. To follow its expression in tobacco leaves, transgenic plants expressing GUS under the control of the LM1 promoter were made. Expression of LM1-GUS was largely restricted to actively growing leaf regions, suggesting possible involvement of active cell division and plant growth regulators in LeKAPalpha 1 expression.
Mizrachy
Liat
L
Department of Genetics, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel.
Dabush
David
D
Levy
Yael
Y
Aloni
Roni
R
Altman
Arie
A
Gafni
Yedidya
Y
D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000032:analysis / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000737:chemistry; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017384:Sequence Deletion / Q000235:genetics; D028901:alpha Karyopherins / Q000235:genetics* / Q000502:physiology
D028901:alpha Karyopherins; D005966:Glucuronidase