Competition between wild-type virus and a reassortant from subgroups I and II of CMV and activation of antiviral responses in cowpea.
To investigate the interactions between RNA3 and RNA4 from subgroups I and II in mixed infections, accumulation of CMV RNA were analyzed. In the mixed inoculation assays with CMV-LE (LE, subgroup I) and a reassortant LLm consisting of RNA1 and RNA2 from LE, and RNA3 from CMV-m2 (m2, subgroup II), LE RNA3 and RNA4 could systemically spread in the plants, whereas those of m2 could not. Furthermore, accumulation of virus short RNA and a cowpea-encoded RNA-directed RNA polymerase gene (VuRdRP1) mRNA were found in the plants, suggesting that VIGS and/or distinct antiviral responses (was) were activated by infection with CMV.
Takeshita
M
M
Laboratory of Plant Pathology, Faculty of Agriculture, Graduate School of Kyushu University, Fukuoka, Japan. minorutk@agr.kyushu-u.ac.jp
Kikuhara
K
K
Kuwata
S
S
Furuya
N
N
Takanami
Y
Y
D017799:Cucumovirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007887:Fabaceae / Q000276:immunology / Q000821:virology*; D020868:Gene Silencing; D018515:Plant Leaves / Q000276:immunology / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D011995:Recombination, Genetic
D012333:RNA, Messenger; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase