A leader intron and 115-bp promoter region necessary for expression of the carnation S-adenosylmethionine decarboxylase gene in the pollen of transgenic tobacco.
The expression of CSDC9 encoding S-adenosylmethionine decarboxylase (SAMDC) is developmentally and spatially regulated in carnation. To examine the regulation of the SAMDC gene, we analyzed the spatial expression of CSDC9 with a 5'-flanking beta-glucuronidase fusion in transgenic tobacco plants. GUS was strongly expressed in flower, pollen, stem and vein of cotyledons. Expression in both anther and stigma was under developmental control; analysis of a series of mutants with deletions of the 5'-flanking region demonstrated differential activation in petal, anther, stigma and pollen grains. All the major cis-regulatory elements required for pollen-specific transcription were located in the upstream region between -273 and -158. This region contains four putative elements related to gibberellin induction (pyrimidine boxes, TTTTTTCC and CCTTTT) and pollen-specific expression (GTGA and AGAAA). In addition, the first 5'-leader intron was necessary for tissue-specific expression.
Kim
Young Jin
YJ
Department of Biology, Yonsei University, Seoul 120-749, Republic of Korea.
Lee
Sun Hi
SH
Park
Ky Young
KY
D024506:5' Flanking Region; D012437:Adenosylmethionine Decarboxylase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D029749:Dianthus / Q000033:anatomy & histology / Q000737:chemistry / Q000235:genetics*; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005966:Glucuronidase / Q000378:metabolism; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D011058:Pollen / Q000166:cytology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017384:Sequence Deletion; D016385:TATA Box; D014026:Nicotiana / Q000235:genetics
D018744:DNA, Plant; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase; D012437:Adenosylmethionine Decarboxylase