Human immunodeficiency virus type 1 Nef domains required for disruption of major histocompatibility complex class I trafficking are also necessary for coprecipitation of Nef with HLA-A2.
Human immunodeficiency virus type 1 (HIV-1) Nef is a critical protein that is necessary for HIV pathogenesis. Its roles include the disruption of major histocompatibility complex class I (MHC-I) and CD4 trafficking to promote immune evasion and viral spread. Mutational analyses have revealed that separate domains of Nef are required to affect these two molecules. To further elucidate how Nef disrupts MHC-I trafficking in T cells, we examined the role of protein domains that are required for this function (N-terminal alpha helix, polyproline, acidic, and oligomerization domains). We found that each of these regions was required for Nef to disrupt the transport of HLA-A2 to the cell surface and for Nef to coprecipitate with HLA-A2.
Williams
Maya
M
Graduate Program in Cellular and Molecular Biology, The University of Michigan, Ann Arbor, Michigan 48109, USA.
Roeth
Jeremiah F
JF
Kasper
Matthew R
MR
Filzen
Tracey M
TM
Collins
Kathleen L
KL
D000256:Adenoviridae / Q000235:genetics; D015693:Gene Products, nef / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D015789:HLA-A2 Antigen / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D013601:T-Lymphocytes / Q000378:metabolism; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D015789:HLA-A2 Antigen; D015395:Histocompatibility Antigens Class I; D054311:nef Gene Products, Human Immunodeficiency Virus