[The influence of the peptide bioregulator prostamax on heterochromatin of human lymphocytes in situ].
It was shown that chromatin contained in human lymphocytes has two stages of denaturation: with T(d)VII = 94.4 degrees C, Q(d)VII = 50.8 J/g DNA, and T(d)VIII = 105.1 degrees C Q(d)VIII = 44.9 J/g DNA. The peptide bioregulator prostamax causes a redistribution of heat among endotherms T(d)III and T(d)IV and a shift of both endotherms to low temperatures by 2.9 and 1.0 degrees C, respectively. It was supposed that the redistribution of heat among endotherms is connected with a partial relaxation of the 30-nm-thick fiber in the 10-nm filament. A weak decrease in T(d)VIII and T(d)VII of lymphocytes treated with prostamax compared to untreated ones is connected with small structural changes of nucleosomal organization in the 10-nm filament and 30-nm-thick fiber.
Meskhi
T
T
Khachidze
D
D
Barbakadze
Sh
Sh
Madzhagaladze
G
G
Gorgoshidze
M
M
Monaselidze
D
D
Lezhava
T
T
Tadumadze
N
N
D000368:Aged; D002152:Calorimetry, Differential Scanning; D002478:Cells, Cultured; D006570:Heterochromatin / Q000737:chemistry*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008875:Middle Aged; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009707:Nucleosomes / Q000737:chemistry / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology*; D013816:Thermodynamics
D006570:Heterochromatin; D009707:Nucleosomes; D009842:Oligopeptides; C500342:Prostamax