title: Xylanase production by Trichoderma reesei Rut C-30 grown on L-arabinose-rich plant hydrolysates.
abstract: The suitability of L-arabinose-rich plant hydrolysates as carbon sources and inducers of xylanase production in Trichoderma reesei Rut C-30 was tested. Significantly higher xylanase activities were obtained in cultures on oat husk and sugar beet pulp hydrolysates than on lactose. In batch culture with oat husk hydrolysate and lactose, the xylanase activity was about 9 times higher ( approximately 510 IU/ml) than in lactose ( approximately 60 IU/ml). Even higher xylanase activity ( approximately 630 IU/ml) was obtained when the batch cultivations were done on sugar beet pulp hydrolysate and lactose. In a fed-batch culture using oat husk hydrolysate-lactose the xylanase activity was as high as 1350 IU/ml in 4 days. The cellulase production clearly decreased when T. reesei was cultured on both hydrolysates compared to the cultivation on lactose. Moreover, the relative amounts of the xylanases I-III were similar regardless the used carbon source.
authors.affiliation: Laboratory of Bioprocess Engineering, Helsinki University of Technology, P.O. Box 6100, FIN-02015 Espoo, Finland.
authors.forename: Hairong
authors.initials: H
authors.lastname: Xiong
authors.forename: Niklas
authors.initials: N
authors.lastname: von Weymarn
authors.forename: Ossi
authors.initials: O
authors.lastname: Turunen
authors.forename: Matti
authors.initials: M
authors.lastname: Leisola
authors.forename: Ossi
authors.initials: O
authors.lastname: Pastinen
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D007785:Lactose / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D014242:Trichoderma / Q000235:genetics / Q000378:metabolism*
chemical_list: D001089:Arabinose; D043364:Endo-1,4-beta Xylanases; D007785:Lactose