[Pharmacokinetics of patchouli alcohol and patchouli alcohol in patchouli oil after iv administrated to rats].
OBJECTIVE
To develop a capillary gas chromatographic method for the determination and pharmacokinetic study of patchouli alcohol in rat plasma after iv administration.


METHODS
The drug was extracted with ethyl acetate. Eugenol was used as internal standard. The separation was carried out on a HP-5MS quartz capillary column, with high-purity nitrogen as carrier gas and flame ionization detector (FID) as detector. The column temperature was maintained at 80 degrees C for 1 min and then programmed to 200 degrees C at a rate of 15 degrees C x min(-1); it was held at 200 degrees C for 1 min, and then programmed to 290 degrees C at a rate of 60 degrees C x min(-1); the final temperature was held for 1 min. The temperature of both injector and detector was set at 290 degrees C.


RESULTS
The standard curve was linear from 25 to 5 000 microg x L(-1) in rat plasma. The recovery of this method was from 90.0% to 110.0% with satisfactory relative standard deviation (RSD) less than 10.0%. The pharmacokinetic parameters demonstrated patchouli alcohol were consistent with the two-compartment open model and showed linear pharmacokinetics. The T1/2beta, AUC and MRT of patchouli alcohol in patchouli oil were all higher than that of patchouli alcohol.


CONCLUSIONS
This method is quick, precise and reliable. The pharmacokinetics of patchouli alcohol is different from that of patchouli alcohol in patchouli oil.
Yang
Fu-chuan
FC
Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Xu
Li-zhen
LZ
Zou
Zhong-mei
ZM
Yang
Shi-lin
SL
D000818:Animals; D019540:Area Under Curve; D007275:Injections, Intravenous; D019686:Lamiaceae / Q000737:chemistry; D008297:Male; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*
D009822:Oils, Volatile; C483659:Patchouliol; D012717:Sesquiterpenes; C052983:patchouli alcohol