Acetylcholinesterase inhibitors from the aerial parts of Corydalis speciosa.
In a bioassay-guided search for acetylcholinesterase inhibitors from Korean natural resources, four isoquinoline alkaloids, corynoxidine (1), protopine (2), palmatine (3), and berberine (4) have been isolated from the methanolic extract of the aerial parts of Corydalis speciosa. Structures of these compounds were elucidated on the basis of spectroscopic techniques. These compounds inhibited acetylcholinesterase activity in a dose-dependent manner, and the IC50 values of compounds 1-4 were 89.0, 16.1, 5.8, and 3.3 microM, respectively.
Kim
Dae Keun
DK
College of Pharmacy, Woosuk University, Samrye 565-701, Korea. dkkim@woosuk.ac.kr
Lee
Ki Taek
KT
Baek
Nam-In
NI
Kim
Sung-Hoon
SH
Park
Hee Wook
HW
Lim
Jong Pil
JP
Shin
Tae Yong
TY
Eom
Dong Ok
DO
Yang
Jae Heon
JH
Eun
Jae Soon
JS
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D053119:Benzophenanthridines; D001599:Berberine / Q000737:chemistry / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031311:Corydalis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D001599:Berberine; D000110:Acetylcholinesterase; C005413:palmatine; C009093:protopine