title: [A two-dimensional reference map of mouse ovary proteins].
abstract: OBJECTIVE
  To perform a preliminary proteomic analysis of mouse ovaries and to study the protein's function in mouse ovary.
  
  
  METHODS
  The two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) were used to analyze mouse ovarian proteome. A 12.5% sodium dodecyl sulfate (SDS) reference gel was generated by immobilized pH gradient isoelectric focusing of mouse ovary proteins in a non-linear gradient (pH 3-10). And GRP78 was selected to perform with immunohistochemistry within mouse ovaries.
  
  
  RESULTS
  Based on peptide mass fingerprinting, 52 proteins were identified and classified into seven functional groups: Cell/organism defense and antioxidant, cell signaling/communications proteins, cell structure/motility proteins, metabolism proteins, RNA synthesis processing, protein synthesis and processing, and unclassified proteins. The immunoreactivity of GRP78 was detected in GCs in the follicular, and with during GCs Luteinizing in the menstrual cycle, the protein expression (brown) increased continually and came to a head when ovulation happened.
  
  
  CONCLUSIONS
  This work provides a first step toward the establishment of a systematic ovary protein database and stands as a valuable resource for molecular analyses of normal and pathologic conditions affecting mouse ovaries.
authors.affiliation: Center of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ma
authors.forename: Yun
authors.initials: Y
authors.lastname: Qian
authors.forename: Man-xin
authors.initials: MX
authors.lastname: Xie
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hou
authors.forename: Yan
authors.initials: Y
authors.lastname: Meng
authors.forename: Jin-yong
authors.initials: JY
authors.lastname: Liu
authors.forename: Yong-ning
authors.initials: YN
authors.lastname: Zhai
authors.forename: Ye-fei
authors.initials: YF
authors.lastname: Zhu
authors.forename: Zuo-min
authors.initials: ZM
authors.lastname: Zhou
authors.forename: Jia-hao
authors.initials: JH
authors.lastname: Sha
authors.forename: Jia-yin
authors.initials: JY
authors.lastname: Liu
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D006360:Heat-Shock Proteins / Q000032:analysis; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D018832:Molecular Chaperones / Q000032:analysis; D010053:Ovary / Q000737:chemistry* / Q000166:cytology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D018832:Molecular Chaperones; D020543:Proteome