title: Quality of milk and of Canestrato Pugliese cheese from ewes exposed to different ventilation regimens.
abstract: Effects of ventilation regimen on the quality of ewes' milk and on proteolysis in Canestrato Pugliese cheese during ripening were studied. Cheeses were manufactured from the bulk milk of Comisana ewes subjected to three different ventilation regimens, which were designated low (LOV, 23 m3/h per ewe), moderate (MOV, 47 m3/h per ewe) and programmed ventilation regimen (PROV, 73 m3/h per ewe; fan set to maintain 70% relative humidity). Bulk milk was analysed for chemical and microbial composition, renneting parameters and plasmin-plasminogen activities. At 1, 15, 30 and 45 d of ripening, the cheeses were analysed for gross chemical composition, nitrogen fractions, and plasmin and plasminogen activities. The pH 4.6-insoluble nitrogen fractions were analysed by urea-PAGE. Free amino acid content was determined at the end of ripening. Lower concentrations of bulk milk somatic cell count (BMSCC) and of mesophilic bacteria were found in the MOV group than in the LOV and the PROV groups. A lower plasminogen (PG) to plasmin (PL) ratio (PG/PL) was observed in the MOV and PROV than in the LOV cheeses. Irrespective of treatment, PL activity in cheeses was higher at 15d of ripening, while a sudden decrease of PL and PG activities was observed at 30 d, which was associated with a marked increase in non-protein nitrogen. The peptide profile characterized in the urea-PAGE showed a greater intensity of alpha- and beta-CN hydrolysis in the MOV than in the PROV and LOV cheeses. The results provide evidence that a proper ventilation regimen is critical for optimizing the hygienic quality of milk and the proteolysis of Canestrato Pugliese cheese during ripening.
authors.affiliation: Dipartimento PRIME Università di Foggia, Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Albenzio
authors.forename: Rosaria
authors.initials: R
authors.lastname: Marino
authors.forename: Mariangela
authors.initials: M
authors.lastname: Caroprese
authors.forename: Antonella
authors.initials: A
authors.lastname: Santillo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Annicchiarico
authors.forename: Agostino
authors.initials: A
authors.lastname: Sevi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D002364:Caseins / Q000032:analysis / Q000378:metabolism; D002452:Cell Count; D002611:Cheese / Q000032:analysis; D003612:Dairying / Q000379:methods; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D005511:Food Handling / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007558:Italy; D008892:Milk / Q000737:chemistry / Q000166:cytology / Q000382:microbiology; D009584:Nitrogen / Q000032:analysis; D010447:Peptide Hydrolases / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism; D011786:Quality Control; D012756:Sheep; D014691:Ventilation / Q000379:methods*
chemical_list: D000596:Amino Acids; D002364:Caseins; D010958:Plasminogen; D010447:Peptide Hydrolases; D005341:Fibrinolysin; D009584:Nitrogen