Comparison of Japanese isolates of Coxiella burnetii by PCR-RFLP and sequence analysis.
The genetic variation of Japanese isolates of Coxiella burnetii, the agent of Q fever, was found for the first time. Forty-nine out of 72 isolates had the chronic pattern of the isocitrate hydrogenase gene. Sequence analysis revealed that the isolates have a specific nucleotide sequence. The putative amino acid sequence was the same as that of chronic reference strains. These results suggest the variation of C. burnetii isolates in Japan.
Andoh
Masako
M
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu 501-1193, Japan.
Nagaoka
Hiromi
H
Yamaguchi
Tsuyoshi
T
Fukushi
Hideto
H
Hirai
Katsuya
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016997:Coxiella burnetii / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011778:Q Fever / Q000382:microbiology*; D016415:Sequence Alignment
D004269:DNA, Bacterial; D007521:Isocitrate Dehydrogenase