[Detection of P2z/P2x7 receptors].
OBJECTIVE
To detect the P2z/P2x7 receptors of human leukocytes.


METHODS
Re-suspending different kinds of leukemic cells, including CEM, THP-1, HL-60, NB4, Dami/Damin and Raji cells in buffer; adjusting their concentration to 2 x 10(7)/ml, and then detecting the P2z/P2x7 receptors respectively.


RESULTS
(1) It was found that 0.4 mmol/L ATP enhanced obviously the fluorenscence strength of THP-1, NB4 and HL-60 cells, the activity rates were 54.91%, 39.45% and 30.46%, respectively. But for CEM, Dam and Raji cells, the activity rates were as low as 10.43%, 13.21% and 2.67%, respectively. KN-62 inhibited obviously the fluorescence strength of THP-1 cells, the inhibition rate reached 88.74%, but for NB4 and HL-60 cells the inhibition rates were only 15.81% and 12.50% respectively, and Raji cells were not affected by KN-62. (2) All the leukemic cells detected with polyclonal antibody were found with positive result.


CONCLUSIONS
THP-1 cells have P2z/P2x7 receptors. The receptors in NB4 and HL-60 belong to P2 receptor family, but they are not P2z/P2x7 receptors. The polyclonal antibody used in immunohistochemical ABC method is not specific to P2z/P2x7 receptors.
Peng
Liming
L
Clinical Laboratory, West China Hospital of Sichuan University, Chengdu 610041, China.
Yang
Hanshuo
H
Zhang
Qin
Q
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology; D018048:Receptors, Purinergic P2 / Q000032:analysis* / Q000235:genetics; D058486:Receptors, Purinergic P2X7; D014407:Tumor Cells, Cultured
C546468:P2RX7 protein, human; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate