AIF and EndoG in noise-induced hearing loss.
Apoptosis plays a critical role in neuronal cell death, including sensory cell death in noise-induced hearing loss. The purpose of this study is to investigate the role of a caspase-independent apoptotic pathway, involving apoptosis-inducing factor (AIF) and endonuclease G (EndoG) in cell death in the inner ear after intense noise exposure (120 dB SPL, 4 kHz OBN, 5 h). EndoG was translocated to the nucleus after noise trauma, whereas immunostaining for AIF was expressed only in the cytosol. These findings indicate that EndoG is a factor in noise-induced caspase-independent apoptosis. In contrast, AIF may not be involved in cell death, but act as a redox factor in response to noise-induced oxidant stress.
Yamashita
Daisuke
D
Kresge Hearing Research Institute, University of Michigan, 1301 E. Ann Street, Ann Arbor, MI 48109 0506, USA.
Miller
Josef M
JM
Jiang
Hong-Yan
HY
Minami
Shujiro B
SB
Schacht
Jochen
J
D000818:Animals; D051033:Apoptosis Inducing Factor; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D005420:Flavoproteins / Q000378:metabolism*; D006168:Guinea Pigs; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism*; D009622:Noise / Q000009:adverse effects; D009925:Organ of Corti / Q000166:cytology / Q000378:metabolism; D011897:Random Allocation; D013997:Time Factors
D051033:Apoptosis Inducing Factor; D005420:Flavoproteins; D008565:Membrane Proteins; D004706:Endodeoxyribonucleases; C052391:endonuclease G