Angiotensin II type 1 receptor mRNA and its protein expression in human pancreatic cancer cell lines.
OBJECTIVE
The incidence of pancreatic cancer is increasing in China, and in many patients the surrounding lymphatics have already been invaded and there is blood-borne metastasis at the time of diagnosis. Additionally, pancreatic cancer is largely refractory to conventional therapies. Therefore, to improve its prognosis, it is important to resolve the problem of its growth. Angiotensin II type 1 receptor (AT1) stimulates the growth and angiogenesis of pancreatic cancer and a selective AT1 antagonist could inhibit these effects. The present study aimed to investigate the expression of AT1 in pancreatic cancer cell lines to provide the theoretical basis for its treatment.


METHODS
The pancreatic cancer cell lines were SW1990, PaTu8988s and PANC-1. RT-PCR was used to detect the AT1 mRNA expression, and ABC immunocytochemical staining and SDS-PAGE were used to detect the expression of AT1 protein.


RESULTS
Both AT1 mRNA and protein were expressed in all three cell lines. The AT1 protein was found on the cell membrane and in the cytoplasm of these cells. The AT1 protein (44 x 10(3)) was also demonstrated by SDS-PAGE.


CONCLUSIONS
The results suggest that AT1 plays an important role in the growth of pancreatic cancer and its inhibition may be a therapeutic strategy.
Jiang
Hua
H
Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, China. jiangh66@yahoo.com
Li
Zhao Shen
ZS
Xu
Guo Ming
GM
Tu
Zhen Xing
ZX
Gong
Yan Fang
YF
D045744:Cell Line, Tumor; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1