The effects of parenteral lipid emulsions on cancer and normal human colon epithelial cells in vitro.
Differences in lipid metabolism of tumor and normal tissues suggest a distinct response to available lipid compounds. In this study, the in vitro effects of five types of commercial parenteral lipid emulsions were investigated on human cell lines derived from normal fetal colon (FHC) or colon adenocarcinoma (HT-29). Changes of the cellular lipid fatty acid content, cell oxidative response, and the cell growth and death rates were evaluated after 48 h. No effects of any type of emulsions were detected on cell proliferation and viability. Compared to the controls, supplementation with lipid emulsions resulted in a multiple increase of linoleic and linolenic acids in total cell lipids, but the content of arachidonic, eicosapentaenoic, and docosahexaenoic acids decreased particularly in HT-29 cells. The concentration of emulsions which did not affected HT-29 cells increased the percentage of floating and subG0/G1 FHC cells probably due to their higher reactive oxygen species production and lipid peroxidation. Co-treatment of cells with antioxidant Trolox reduced the observed effects. Our results imply that lipid emulsions can differently affect the response of colon cells of distinct origin.
Hofmanová
J
J
Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65 Brno, Královopolská 135, Czech Republic.
Zadák
Z
Z
Hyspler
R
R
Mikeska
J
J
Zdánský
P
P
Vaculová
A
A
Netíková
J
J
Kozubík
A
A
D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013024:Soybean Oil / Q000494:pharmacology; D014280:Triglycerides / Q000494:pharmacology
D005217:Fat Emulsions, Intravenous; D005227:Fatty Acids; D017382:Reactive Oxygen Species; D014280:Triglycerides; D013024:Soybean Oil