A single dose of erythropoietin attenuates lipid peroxidation in experimental liver ischemia-reperfusion injury in the rat fetus.
OBJECTIVE
The aim of the study was investigate the effectiveness of recombinant human erythropoietin (r-Hu-EPO) in attenuating the severity of experimental liver ischemic injury in fetal rats.


METHODS
The animals were divided randomly into four groups. In the control group, fetal whole liver tissue was taken immediately after laparotomy from pregnant animals. In the ischemia-reperfusion (I/R) group, tissue samples were taken immediately after I/R injury. In the vehicle group, 0.4 ml of human serum albumin solution and in the treatment group, r-Hu-EPO (5000 IU/kg) in 0.4 ml of human serum albumin solution were given intraperitoneally, 30 min before I/R injury, as a single dose. Thiobarbituric acid-reactive substances (TBARS) were estimated to demonstrate lipid peroxidation.


RESULTS
Lipid peroxidation byproducts increased after I/R injury. Administration of r-Hu-EPO reduced TBARS after I/R injury.


CONCLUSIONS
Further investigations are needed to understand the mechanism of the hepatoprotective effect of erythropoietin and the clinical importance of ischemic liver injury in the fetus.
Solaroglu
A
A
Social Insurance Institution, Ankara Maternity and Women's Health Teaching Hospital, Department of Obstetrics and Gynecology, Turkey.
Dede
F S
FS
Okutan
E
E
Bayrak
A
A
Haberal
A
A
Kilinc
K
K
D000818:Animals; D004334:Drug Administration Schedule; D004921:Erythropoietin / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000098:blood supply*; D006801:Humans; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000098:blood supply / Q000196:embryology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D015427:Reperfusion Injury / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D011994:Recombinant Proteins; D017392:Thiobarbituric Acid Reactive Substances; D004921:Erythropoietin