Recovery of periodontopathogenic bacteria from embalmed human cadavers.
There is recent interest in recovery of periodontopathogenic bacteria from arterial and bronchial tissues to identify a link between periodontal and cardiovascular or pulmonary diseases. This interest could provide a useful clinical correlation exercise for gross anatomy. Our objective was to perform a feasibility study to determine whether these bacteria could be recovered from two sites within eight (4 dentate, 4 edentulous) human embalmed cadavers from an anatomical dissection laboratory. Bacterial samples were collected from the right coronary artery and the right superior secondary bronchus and assayed for the presence and concentrations of the DNA of A. actinomycetemcomitans, E. corrodens, C. rectus, P. intermedia, P. gingivalis, B. forsythus, T. denticola, and F. nucleatum. Frequencies were compared using a Kruskal-Wallis H-test. Correlations between the presence of teeth, bacterial species, and site were determined by a Spearman's rho correlation test. A. actinomycetemcomitans and B. forsythus frequencies were different between the sites in edentulous subjects (P <0.05); the frequency of B. forsythus was different in dentate and edentulous subjects at the bronchus site (P <0.05). Numerous significant correlations were identified between strains of bacteria, site, and presence of teeth. Thus, it is possible for the DNA of periodontopathogenic bacteria to be recovered from human embalmed cadavers. Collection and identification of these bacteria from these cadavers could be a useful clinical correlation exercise for dental students in a gross anatomy class.
Wood
Nelson
N
Department of Periodontics, School of Dentistry, University of Mississippi Medical Center, Jackson, Mississippi.
Johnson
Roger B
RB
D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001980:Bronchi / Q000382:microbiology; D002102:Cadaver; D003324:Coronary Artery Disease / Q000382:microbiology; D003331:Coronary Vessels / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000150:complications / Q000382:microbiology*
D004269:DNA, Bacterial