Extracting DNA twist rigidity from experimental supercoiling data.
We use an elastic rod model with contact to study the extension versus rotation diagrams of single supercoiled DNA molecules. We reproduce quantitatively the supercoiling response of overtwisted DNA and, using experimental data, we obtain an estimate of the effective supercoiling radius and of the twist rigidity of B-DNA. We find that the twist rigidity of DNA seems to vary widely with the nature and concentration of the salt buffer in which it is immersed.
Neukirch
Sébastien
S
Institute for Mathematics B, Swiss Federal Institute of Technology, Lausanne, Switzerland.
D004247:DNA / Q000737:chemistry; D004278:DNA, Superhelical / Q000737:chemistry*; D004548:Elasticity; D008956:Models, Chemical; D009690:Nucleic Acid Conformation
D004278:DNA, Superhelical; D004247:DNA