Multiplexed MS/MS in a quadrupole ion trap mass spectrometer.
A multiplexing method for performing MS/MS on multiple peptide ions simultaneously in a quadrupole ion trap mass spectrometer (QITMS) has been developed. This method takes advantage of the inherent mass bias associated with ion accumulation in the QITMS to encode the intensity of precursor ions in a way that allows the corresponding product ions to be identified. The intensity encoding scheme utilizes the Gaussian distributions that characterize the relationship between ion intensities and rf trapping voltages during ion accumulation. This straightforward approach uses only two arbitrary waveforms, one for isolation and one for dissociation, to gather product ion spectra from N precursor ions in as little as two product ion spectra. In the example used to illustrate this method, 66% of the product ions from five different precursor peptide ions were correctly correlated using the multiplexing approach. Of the remaining 34% of the product ions, only 6% were misidentified, while 28% of the product ions failed to be identified because either they had too low intensity or they had the same m/z ratio as one of the precursor ions or the same m/z ratio as a product ion from a different precursor ion. This method has the potential to increase sample throughput, reduce total analysis times, and increase signal-to-noise ratios as compared to conventional MS/MS methods.
Wilson
Jonathan
J
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Vachet
Richard W
RW
D045304:Cytochromes c / Q000032:analysis; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D016011:Normal Distribution; D010455:Peptides / Q000032:analysis; D015203:Reproducibility of Results
D007477:Ions; D010455:Peptides; D045304:Cytochromes c