title: Engineering stereocontrol into an aldolase-catalysed reaction.
abstract: A novel thermostable aldolase has been developed for synthetic application, and substrate engineering has been used to induce stereocontrol into aldol reactions of this naturally-promiscuous enzyme.
authors.affiliation: Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath, UK BA2 7AY.
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Lamble
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Danson
authors.forename: David W
authors.initials: DW
authors.lastname: Hough
authors.forename: Steven D
authors.initials: SD
authors.lastname: Bull
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D001698:Biomedical Engineering / Q000379:methods*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004795:Enzyme Stability; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D013237:Stereoisomerism; D016944:Sulfolobus / Q000201:enzymology / Q000235:genetics
chemical_list: D000447:Aldehydes; C116609:3-hydroxybutanal; D005634:Fructose-Bisphosphate Aldolase