Rapid construction of capsid-modified adenoviral vectors through bacteriophage lambda Red recombination.
There are extensive efforts to develop cell-targeting adenoviral vectors for gene therapy wherein endogenous cell-binding ligands are ablated and exogenous ligands are introduced by genetic means. Although current approaches can genetically manipulate the capsid genes of adenoviral vectors, these approaches can be time-consuming and require multiple steps to produce a modified viral genome. We present here the use of the bacteriophage lambda Red recombination system as a valuable tool for the easy and rapid construction of capsid-modified adenoviral genomes.
Campos
Samuel K
SK
Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77251, USA.
Barry
Michael A
MA
D000256:Adenoviridae / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D001710:Biotin / Q000235:genetics; D019921:Biotinylation; D015153:Blotting, Western; D002213:Capsid; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005818:Genetic Engineering; D015316:Genetic Therapy; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D008024:Ligands; D008957:Models, Genetic; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D013997:Time Factors
D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D001710:Biotin; D004247:DNA