title: Monocyte switch in neonates: high phagocytic capacity and low HLA-DR expression in VLBWI are inverted during gestational aging.
abstract: Pre-term neonates are at high risk to develop early-onset sepsis which possibly is caused by an immature immune system. Monocytes play a pivotal role as professional phagocytic and antigen-presenting cells in the innate immunity. In the present study, we investigated in monocytes from cord blood the expression of human leukocyte antigen (HLA)-DR as a marker for antigen-presenting capability, the expression of the high-affinity receptor for IgG (FcgammaRI/CD64), and the capacity to phagocytize non-opsonized Escherichia coli. We compared 70 infants in three groups according to their gestational age (group I: 20 very low birth weight infants (VLBWI), 24-31 weeks of gestation; group II: 25 pre-term infants, 32-36 weeks of gestation, and group III: 25 term neonates). The expression of CD64 as well as the phagocytic capacity of monocytes from cord blood were highest in VLBWI (p < 0.05 and p < 0.01, respectively). In contrast, HLA-DR expression was significantly (p < 0.05) diminished in VLBWI, which possibly leads to a reduced antigen-presenting capacity. We conclude that monocytes have different functional properties during gestational aging, which perhaps participate in the high incidence of infections in VLBWI.
authors.affiliation: Department of Pediatric Surgery, University of Graz, Graz, Austria.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Hallwirth
authors.forename: Gerhard
authors.initials: G
authors.lastname: Pomberger
authors.forename: Arnold
authors.initials: A
authors.lastname: Pollak
authors.forename: Erich
authors.initials: E
authors.lastname: Roth
authors.forename: Andreas
authors.initials: A
authors.lastname: Spittler
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D004926:Escherichia coli / Q000276:immunology; D005312:Fetal Blood / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D005865:Gestational Age; D006684:HLA-DR Antigens / Q000096:biosynthesis* / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology / Q000502:physiology*
chemical_list: D006684:HLA-DR Antigens