title: Automated yeast two-hybrid screening for nuclear receptor-interacting proteins.
abstract: High throughput analysis of protein-protein interactions is an important sector of hypothesis-generating research. Using an improved and automated version of the yeast two-hybrid system, we completed a large interaction screening project with a focus on nuclear receptors and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens resulted in 6425 potential interacting protein fragments involved in 1613 different interaction pairs. We show that simple statistical parameters can be used to narrow down the data set to a high confidence set of 377 interaction pairs where validated interactions are enriched to 61% of all pairs. Within the high confidence set, there are 64 novel proteins potentially binding to nuclear receptors or their cofactors. We discuss several examples of high interest, and we expect that communication of this huge data set will help to complement our knowledge of the protein interaction repertoire of this family of transcription factors and instigate the characterization of the various novel candidate interactors.
authors.affiliation: PheneX Pharmaceuticals AG, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Albers
authors.forename: Harald
authors.initials: H
authors.lastname: Kranz
authors.forename: Ingo
authors.initials: I
authors.lastname: Kober
authors.forename: Carmen
authors.initials: C
authors.lastname: Kaiser
authors.forename: Martin
authors.initials: M
authors.lastname: Klink
authors.forename: Jörg
authors.initials: J
authors.lastname: Suckow
authors.forename: Rainer
authors.initials: R
authors.lastname: Kern
authors.forename: Manfred
authors.initials: M
authors.lastname: Koegl
mesh_terms: D001331:Automation; D002467:Cell Nucleus / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D018076:DNA, Complementary / Q000378:metabolism; D019992:Databases as Topic; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry* / Q000378:metabolism; D013223:Statistics as Topic / Q000379:methods*; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D020543:Proteome; D018160:Receptors, Cytoplasmic and Nuclear