[Expression of bax protein in the cartilage cells of epiphyseal plate].
OBJECTIVE
This experiment was designed to investigate the expression of Bax in the chondrocytes of the epiphyseal plate cartilage.


METHODS
Immunocytochemistry ABC method was used to determine the distribution of Bax immunoreactivity in the epiphyseal plate cartilage of rats.


RESULTS
Bax immunoreactivity was non-uniformly distributed throughout the epiphyseal cartilage. There was no Bax expression in chondrocytes under the perichondrium. Bax expression was observed in the resting chondrocytes of epiphysis; it increased in the proliferative chondrocytes of epiphysis, and immunoreactivity was mainly in the cytoplasm of chondrocytes; Bax expression was maximal in the hypertrophic chondrocytes of epiphysis, and immunoreactivity was mainly in the cytoplasm and nuclei of chondrocytes.


CONCLUSIONS
These immunocytochemical data indicate that Bax may promote chondrocytes apoptosis of epiphysis through an accumulative increase in quantity.
Zhang
Xiao
X
Department of Experiment Technique of Chengdu Military College, The 3rd Military Medical University, Chengdu 610083, China.
Li
Ruixiang
R
Yang
Huijun
H
Gao
Li
L
Yang
Shuxia
S
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology; D019902:Chondrocytes / Q000378:metabolism*; D006132:Growth Plate / Q000378:metabolism*; D007150:Immunohistochemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein