Visualization and examination of the meiotic spindle in human oocytes with polscope.
OBJECTIVE
The effect of detectability/location of meiotic spindle to fertilization of human MII oocytes and outcome of clinical IVF/ICSI-ET cycles was studied.


METHODS
The location of spindle relative to polar body positioned to 6 o'clock was detected with polscope prior to ICSI in oocytes. Data of 83 IVF/ICSI-ET cycle, including pregnancy results, and a total of 1033 oocytes examined were analyzed retrospectively.


RESULTS
Polar body position does not always accurately predict spindle position. Similar fertilization was observed in the polscope detected and in the control group. The main number of oocytes and spindle positive oocytes collected was higher in the pregnant than in the non-pregnant group. Higher fertilization was found in that group where 2/3-d of the oocytes had detected spindle. The main number of oocytes and spindle positive oocytes obtained decreased with increasing age of patients.


CONCLUSIONS
The presence of birefringent spindle predicts higher fertilization and development and greater probability of pregnancy.
Konc
Janos
J
Infertility and IVF Center of Buda, Saint János Hospital, Budapest, Hungary.
Kanyó
Katalin
K
Cseh
Sandor
S
D000328:Adult; D016022:Case-Control Studies; D019723:Endoscopes; D005260:Female; D005306:Fertilization; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D011256:Pregnancy Outcome; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus / Q000648:ultrastructure*