title: Bxz1, a new generalized transducing phage for mycobacteria.
abstract: We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae. Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10(-8) to 10(-6) per plaque forming unit between strains of M. smegmatis. We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene.
authors.affiliation: Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Sunhee
authors.initials: S
authors.lastname: Lee
authors.forename: Jordan
authors.initials: J
authors.lastname: Kriakov
authors.forename: Catherine
authors.initials: C
authors.lastname: Vilcheze
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Dai
authors.forename: Graham F
authors.initials: GF
authors.lastname: Hatfull
authors.forename: William R
authors.initials: WR
authors.lastname: Jacobs
mesh_terms: D003227:Conjugation, Genetic; D005819:Genetic Markers; D009160:Mycobacteriophages / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D009161:Mycobacterium / Q000235:genetics / Q000821:virology*; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000821:virology*; D010957:Plasmids; D012988:Soil Microbiology; D014161:Transduction, Genetic
chemical_list: D005819:Genetic Markers