title: Genetic and serological characterization of novel serotype G8 bovine group A rotavirus strains isolated in Japan.
abstract: G8 bovine group A rotaviruses isolated in Japan were genetically and serologically characterized. The VP7 gene nucleotide and amino acid sequences revealed high identity with each other. All Japanese G8 strains were classified into the same lineage in the phylogenetic analysis based on VP7 gene sequences. Antisera to four Japanese G8 strains neutralized other G8 strains, but their neutralizing titers were between 8-fold lower and 2-fold higher than homologous strains. These results suggest that the VP7s of Japanese G8 strains have similar genetic and serologic characteristics. Observed differences in the neutralizing abilities of antisera for each strain appear to depend on differences in the P serotypes/genotypes.
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Fukai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Onoda
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sato
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Miura
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle / Q000821:virology*; D002418:Cattle Diseases / Q000821:virology; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D005814:Genes, Viral; D005838:Genotype; D007564:Japan; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000662:veterinary / Q000821:virology; D012703:Serotyping
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus