title: Acetylcholinesterase inhibitors from the aerial parts of Corydalis speciosa.
abstract: In a bioassay-guided search for acetylcholinesterase inhibitors from Korean natural resources, four isoquinoline alkaloids, corynoxidine (1), protopine (2), palmatine (3), and berberine (4) have been isolated from the methanolic extract of the aerial parts of Corydalis speciosa. Structures of these compounds were elucidated on the basis of spectroscopic techniques. These compounds inhibited acetylcholinesterase activity in a dose-dependent manner, and the IC50 values of compounds 1-4 were 89.0, 16.1, 5.8, and 3.3 microM, respectively.
authors.affiliation: College of Pharmacy, Woosuk University, Samrye 565-701, Korea. dkkim@woosuk.ac.kr
authors.forename: Dae Keun
authors.initials: DK
authors.lastname: Kim
authors.forename: Ki Taek
authors.initials: KT
authors.lastname: Lee
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Hee Wook
authors.initials: HW
authors.lastname: Park
authors.forename: Jong Pil
authors.initials: JP
authors.lastname: Lim
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Dong Ok
authors.initials: DO
authors.lastname: Eom
authors.forename: Jae Heon
authors.initials: JH
authors.lastname: Yang
authors.forename: Jae Soon
authors.initials: JS
authors.lastname: Eun
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D053119:Benzophenanthridines; D001599:Berberine / Q000737:chemistry / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031311:Corydalis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D001599:Berberine; D000110:Acetylcholinesterase; C005413:palmatine; C009093:protopine