Enhancement of ajmalicine production in Catharanthus roseus cell cultures with methyl jasmonate is dependent on timing and dosage of elicitation.
The optimum growth stage for enhancing ajmalicine production in Catharanthus roseus cultures with methyl jasmonate (MJ) was after 6 d growth. MJ added at 10 or 100 microm on day 6 gave a maximum ajmalicine production of 10.2 mg l(-1), a 300% increase over that of non-elicited cultures.
Lee-Parsons
Carolyn W T
CW
Chemical Engineering Department, 342 Snell Engineering Center, 360 Huntington Avenue, Northeastern University, Boston, MA 02115-5000, USA. clee@coe.neu.edu.
Ertürk
Seda
S
Tengtrakool
Jennifer
J
D000085:Acetates / Q000008:administration & dosage*; D030008:Catharanthus / Q000187:drug effects* / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D054883:Oxylipins; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism*; D013997:Time Factors
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D046948:Secologanin Tryptamine Alkaloids; C005709:raubasine; C072239:methyl jasmonate