Formation, characterization, and chemistry of undecanoic acid-terminated silicon surfaces: patterning and immobilization of DNA.
This paper describes a simple strategy for DNA immobilization on chemically modified and patterned silicon surfaces. The photochemical modification of hydrogen-terminated Si(111) with undecylenic acid leads to the formation of an organic monolayer covalently attached to the surface through Si-C bonds without detectable reaction of the carboxylic acid group, providing indirect support of a free radical mechanism. Chemical activation of the acid function was achieved by a simple chemical route using N-hydroxysuccinimide (NHS) in the presence of N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride. Single strand DNA with a 5'-dodecylamine group was then coupled to the NHS-activated surface by amide bond formation. Using a previously reported chemical patterning approach, we have shown that DNA can be immobilized on silicon surfaces in spatially well-resolved domains. Methoxytetraethyleneglycolamine was used to inhibit nonspecific adsorption. The resulting DNA-modified surfaces have shown good specificity and chemical and thermal stability under hybridization conditions. The sequential reactions on the surface were monitored by ATR-FTIR, X-ray Photoelectron Spectroscopy, and fluorescence spectroscopy.
Voicu
Raluca
R
Steacie Institute for Molecular Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ontario, K1A 0R6 Canada.
Boukherroub
Rabah
R
Bartzoka
Vasiliki
V
Ward
Tim
T
Wojtyk
James T C
JT
Wayner
Danial D M
DD
D004247:DNA / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D018613:Microscopy, Confocal; D015394:Molecular Structure; D010777:Photochemistry; D012825:Silicon / Q000737:chemistry*; D013053:Spectrophotometry
D005227:Fatty Acids; C016173:undecanoic acid; D004247:DNA; D012825:Silicon