Suppressive effect of a hot water extract of adzuki beans (Vigna angularis) on hyperglycemia after sucrose loading in mice and diabetic rats.
A hot water extract obtained by boiling adzuki beans (Vigna angularis) to produce bean paste for Japanese cake showed inhibitory activity against alpha-glucosidase, alpha-amylase, maltase, sucrase, and isomaltase after HP-20 column chromatography. The IC(50) values for each hydrolylase were 0.78 mg/ml (alpha-amylase), 2.45 mg/ml (maltase), 5.37 mg/ml (sucrase), and 1.75 mg/ml (isomaltase). The active fraction showed potential hypoglycemic activity in both normal mice and streptozotocin (STZ)-induced diabetic rats after an oral administration of sucrose, but did not show any effect on the blood glucose concentration after glucose administration, suggesting that the active fraction suppressed the postprandial blood glucose level by inhibiting alpha-glucosidase and alpha-amylase, irrespective of the endogenous blood insulin level.
Itoh
Tomohiro
T
Imuraya Confectionary Co. Ltd., Takachaya 7-1-1, Tsu, Mie 514-8530, Japan. chuken@imuraya.co.jp
Kita
Nobuyuki
N
Kurokawa
Yuko
Y
Kobayashi
Misato
M
Horio
Fumihiko
F
Furuichi
Yukio
Y
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D005951:Glucose Tolerance Test; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors; D006943:Hyperglycemia / Q000188:drug therapy*; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013395:Sucrose / Q000008:administration & dosage
D001786:Blood Glucose; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013395:Sucrose; D006026:Glycoside Hydrolases