Differential expression of TGF-beta isoforms during differentiation of HaCaT human keratinocyte cells: implication for the separate role in epidermal differentiation.
The three mammalian isoforms of transforming growth factor-beta(TGF-beta1, beta2, beta3) are potent regulators of cell growth, differentiation, and extracellular matrix deposition. To study their role in skin differentiation, we investigated the expression of TGF-beta isoforms on cell growth and differentiation induction of the human keratinocyte cell line, HaCaT by elevating the Ca(2+) concentration. An ELISA and RT-PCR assay revealed secreted TGF-beta 1 protein and TGF-beta 1 mRNA were increased during calcium-induced differentiation. In contrast, major differences were seen for TGF-beta 2 and TGF-beta 3 mRNA which were decreased during differentiation, but TGF-beta 2 and TGF-beta3 protein were not evident on an ELISA. These results suggest different functions for each TGF-beta isoforms in epidermal differentiation, such that TGF-beta 1 is associated with the more differentiated state, and TGF-beta 2 and TGF-beta 3 may be associated the more proliferated state.
Cho
Hang-Rae
HR
Department of Dermatology, College of Medicine, Kyung Hee University, Seoul, Korea.
Hong
Seok-Beom
SB
Kim
Young Il
YI
Lee
Jin-Woo
JW
Kim
Nack-In
NI
D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology*; D020033:Protein Isoforms / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D020033:Protein Isoforms; D016212:Transforming Growth Factor beta