title: Heparan sulfate depletion amplifies TNF-alpha-induced protein leakage in an in vitro model of protein-losing enteropathy.
abstract: Protein-losing enteropathy (PLE), the excessive loss of plasma proteins through the intestine, often correlates with the episodic loss of heparan sulfate (HS) proteoglycans (HSPG) from the basolateral surface of intestinal epithelial cells. PLE onset is often associated with a proinflammatory state. We investigated whether loss of HS or treatment with the proinflammatory cytokine TNF-alpha directly causes protein leakage and whether a combination of both exacerbates this process. We established the first in vitro model of PLE and measured the flux of albumin/FITC through a monolayer of intestinal HT29 or Caco-2 cells grown on transwells and determined the integrity by transepithelial electrical resistance (TER). Loss of HS from the basolateral surface, either by heparanase digestion or by inhibition of HS synthesis, increased albumin flux 1.58 +/- 0.09-fold and reduced TER by 23.4 +/- 6.5%. TNF-alpha treatment increased albumin flux 4.04 +/- 0.03-fold and reduced TER by 75.7 +/- 4.7% but only slightly decreased HS content. The combined effects of HS loss and TNF-alpha treatment were not only additive, but synergistic, with a 7.00 +/- 0.11-fold increase in albumin flux and a 83.9 +/- 8.1% reduction of TER. Coincubation of TNF-alpha with soluble HS or heparin abolished these synergistic effects. Loss of basolateral HS directly causes protein leakage and amplifies the effects of the proinflammatory cytokine TNF-alpha. Our findings imply that loss of HSPGs renders patients more susceptible to PLE and offer a potential explanation for the favorable response some PLE patients have to heparin therapy.
authors.affiliation: The Burnham Institute, Glycobiology and Carbohydrate Chemistry Program, 10901 N. Torrey Pines Rd., La Jolla, California 92037, USA.
authors.forename: Lars
authors.initials: L
authors.lastname: Bode
authors.forename: Erik A
authors.initials: EA
authors.lastname: Eklund
authors.forename: Simon
authors.initials: S
authors.lastname: Murch
authors.forename: Hudson H
authors.initials: HH
authors.lastname: Freeze
mesh_terms: D000418:Albumins / Q000378:metabolism*; D002460:Cell Line; D005966:Glucuronidase / Q000378:metabolism; D006025:Glycosaminoglycans / Q000502:physiology; D019812:Heparan Sulfate Proteoglycans / Q000502:physiology; D006493:Heparin / Q000502:physiology; D006497:Heparan Sulfate / Q000502:physiology*; D006801:Humans; D011504:Protein-Losing Enteropathies / Q000378:metabolism* / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D000099175:Heparanase
chemical_list: D000418:Albumins; D005966:Glucuronidase; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin; D006497:Heparan Sulfate; D014409:Tumor Necrosis Factor-alpha; D000099175:Heparanase