title: The development of immunoassays to identify and quantify species source of gum arabic.
abstract: Gum arabic from Acacia senegal is commonly used as an additive in foodstuffs. Adulteration of gum arabic by other gums is a potential problem for reasons of safety and quality. This study aimed to develop and evaluate the use of enzyme-linked immunosorbent assays (ELISAs) for the detection of potential adulterants of gum arabic. Indirect competitive ELISAs (IC-ELISAs) were developed using the monoclonal antibodies SY CC7 (A. senegal), SY HH3 (Acacia seyal), and SY J1A1 (Combretum erythrophyllum). All IC-ELISAs had a working range of 0.005-10 mg/mL. The antibodies used were tested using the IC-ELISAs for cross-reactivity with other Acacia species and other gums. The antibodies were very specific for their respective antigens. Significant cross-reactivity was found for SY CC7 (between A. senegal and A. melliferae) and SY J1A1 (between C. erythrophyllum and A. seyal). The IC-ELISA was adapted further to test confectionery samples for the presence of gum arabic, which was successful, although recovery rates were reduced. Both IC- and plate trapped antigen ELISA (PTA-ELISA) formats were able to distinguish an adulterated sample of gum arabic when blended with either A. seyal or C. erythrophyllum. The PTA-ELISA was more sensitive for A. seyal than the IC-ELISA, but both were equally sensitive for C. erythrophyllum. The results suggest that the antibodies SY CC7, SY HH3, and SY J1A1 could be used in combination with each other for the detection of potential adulterants of A. senegal and the detection of gum arabic in foodstuffs.
authors.affiliation: Chester Centre for Stress Research, Department of Biological Sciences, University College Chester, Parkgate Road, Chester, CH1 4BJ, United Kingdom.
authors.forename: H Elyse
authors.initials: HE
authors.lastname: Ireland
authors.forename: Andy
authors.initials: A
authors.lastname: Clutterbuck
authors.forename: Jean-Phillipe
authors.initials: JP
authors.lastname: Cloquet
authors.forename: Milo I
authors.initials: MI
authors.lastname: Thurston
authors.forename: Peter A
authors.initials: PA
authors.lastname: Williams
authors.forename: Quentin C
authors.initials: QC
authors.lastname: Cronk
authors.forename: F Molly
authors.initials: FM
authors.lastname: Dewey
authors.forename: John H H
authors.initials: JH
authors.lastname: Williams
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006170:Gum Arabic / Q000032:analysis* / Q000145:classification*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D006170:Gum Arabic