title: A polyclonal antibody directed against syringylpropane epitopes of native lignins.
abstract: With a view to visualizing the ultrastructural distribution of syringyl lignins in secondary plant cell walls, a polyclonal antibody raised from a synthetic DHP polymer consisting only of syringyl propane units was prepared. To test the reactivity of the antiserum, a mini-dot-blot immunoassay reducing the amounts of substrates and antiserum was developed. A characteristic attribute of the S-antiserum appears to be its specific recognition of sequences of three or more consecutive syringyl units. On ultra-thin sections of model plants of Arabidopsis thaliana, Populus and tobacco, the antiserum allowed us to demonstrate a higher concentration of syringyl epitopes in fibres than in vessels. Variations in the distribution pattern of these epitopes between the three plants examined suggest that the synthesis of syringyl lignins in angiosperms depends on the species.
authors.affiliation: Centre de recherche sur les macromolécules végétales, UPR CNRS 5301, BP53, 38041 Grenoble, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Joseleau
authors.forename: Oskar
authors.initials: O
authors.lastname: Faix
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Katia
authors.initials: K
authors.lastname: Ruel
mesh_terms: D000911:Antibodies, Monoclonal; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure*; D000939:Epitopes; D008031:Lignin / Q000032:analysis / Q000276:immunology*; D018514:Plant Structures / Q000737:chemistry / Q000648:ultrastructure*; D011407:Propane
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; C434469:syringyl monolignol; D008031:Lignin; D011407:Propane