title: [DNA damage and apoptosis in L-02 cells induced by chlorination by-product MX of drinking water].
abstract: OBJECTIVE
  To study DNA damage and apoptosis induced by chlorination by-product MX of drinking water.
  
  
  METHODS
  Single cell gel electrophoresis (SCGE) and flow cytometry were used to detect DNA damage and apoptosis in human fetal hepatocytes (L-02) treated with MX.
  
  
  RESULTS
  DNA strand breaks were found to increase in L-02 cells treated with MX in a dose-response manner. On treatment levels of 100 and 300 micromol/L, MX led to significant increase of DNA damage in comparison to the solvent controls (DMSO) (P < 0.05, and P < 0.01 respectively). Statistically significant increase of L-02 cell apoptosis were observed in all MX treated groups in comparison to the solvent controls (DMSO) (P < 0.001).
  
  
  CONCLUSIONS
  Chlorination by-product MX of drinking water could induce obviously DNA damage and apoptosis in L-02 cells.
authors.affiliation: Institute of Chemical Carcinogenesis of Guangzhou Medical College, Guangzhou 510182, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wu
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.forename: Yaling
authors.initials: Y
authors.lastname: Zou
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhou
authors.forename: Wenqing
authors.initials: W
authors.lastname: Lu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005663:Furans / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014881:Water Supply
chemical_list: D005663:Furans; D014874:Water Pollutants, Chemical; C054121:3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone