Gender-limited association of cytotoxic T-lymphocyte antigen-4 (CTLA-4) polymorphism with cord blood IgE levels.
Allergic mechanism has long been attributed to IgE-mediated reaction. The relationship between gene polymorphism and cord blood IgE (CB IgE) is unclear. We investigated whether elevation of CB IgE levels was associated with polymorphisms of cytotoxic T-lymphocyte antigen 4 (CTLA-4) at (-318) CT and (+49) AG positions in a gender-limited fashion. CB IgE levels were determined by Pharmacia CAP system and the CTLA-4 polymorphisms at (-318) and (+49) were determined by restriction fragment length polymorphism (RFLP). A total of 644 consecutive umbilical cord bloods were collected for this study. 32.9% of newborn infants had detectable IgE levels (> or =0.35 kU/l). 25.6% of the male newborns had elevated CB IgE levels (> or =0.5 kU/l) similar to those of the female newborns (22.7%). The CTLA-4 polymorphism at (+49) but not (-318) was significantly associated with elevated CB IgE levels (p = 0.004). The association of CTLA-4 (+49) A allele with elevated CB IgE levels was found only in females. Both male and female infants with different CTLA-4 (-318) genotypes had no difference in the rates of elevated CB IgE levels. A linkage disequilibrium between CTLA-4 (+49) G and (-318) C allele was found in this Chinese population. Subjects with the (+49, GG and -318, CC) genotype had a significantly lower rate of elevated CB IgE levels. Association of the CTLA-4 (+49) polymorphism with elevated CB IgE levels was found only in female infants. Newborn infants with the (+49, GG and -318, CC) genotype tended to have a low rate of elevated CB IgE.
Chang
Jen-Chieh
JC
Department of Pediatrics, Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
Liu
Chieh-An
CA
Chuang
Hau
H
Ou
Chia-Yu
CY
Hsu
Te-Yao
TY
Huang
Eng-Yen
EY
Yang
Kuender D
KD
D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000097:blood* / Q000235:genetics*; D060908:CTLA-4 Antigen; D016009:Chi-Square Distribution; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood* / Q000235:genetics*; D007166:Immunosuppressive Agents / Q000097:blood*; D007231:Infant, Newborn; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics / Q000502:physiology*; D012150:Polymorphism, Restriction Fragment Length; D012737:Sex Factors; D013624:Taiwan
D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D007166:Immunosuppressive Agents; D007073:Immunoglobulin E