[Effect of autoskin grafting in fault hypodermis wound of granulation excision on full-thickness burn healing].
OBJECTIVE
To explore the mechanism of full-thickness burn wound healing with autoskin grafting in fault hypodermis wound of granulation excision and to evaluate its effect.


METHODS
By the techniques of clinical observation, histopathology, immunohistochemistry, TEM and FCM, we observed changes of the activity and structure of grafted skin and the granulation tissue, collagnous fiber,microvessels,the ultramicrostructure of fibroblasts and the expression of basic fibroblast growth factor (bFGF) in the base of autoskin grafting in fault hypodermis wound in burned adult minipigs (Group A), and compared with traditional method of autoskin grafting on the basilar fibrous tissue wound of scraped partly granulation being (Group B) and control group (Group C, without treatment except de-fur).


RESULTS
The grafted skin survived after 3 days of operation, and it had less injury and higher proliferative index (PI) in group A than in group B. The hyperplasia of granulation tissue and vascular endothelial and the expression of bFGF were more evident in group A. After 5 days, the proliferation of endothelial cells and granulation and the protein synthesis of fibroblasts were more active in group A, and at this moment, fresh collagen appeared and proliferated more actively in group B. After 7-14 days, epidermic structure and dermic microvascular density became normal gradually, the granulation on grafting base matured and transformed into fibrous connective tissue in group A. The same change deferred about 2 days in group B. After 21 days, the above pathologic change in group A was less than that in group B. After 30-60 days of operation, Group A achieved much less contraction and transfiguration than Group B, and the grafted skin was tender and movable.


CONCLUSIONS
Autoskin grafting in fault hypodermis wound of granulation excision has a better effect than traditional operation.
Song
Guodong
G
Department of Burn Surgery, Jinan Central Hospital, the Clinical Medical College of Shandong University, Jinan Shandong, PR China.
Zhang
Lei
L
Jia
Jun
J
D000818:Animals; D002056:Burns / Q000601:surgery*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D006097:Granulation Tissue / Q000601:surgery*; D009779:Occlusive Dressings; D016038:Skin Transplantation; D013552:Swine; D013556:Swine, Miniature; D014945:Wound Healing
D016222:Fibroblast Growth Factor 2