Use of the DsRed fluorescent reporter in zebrafish.
Green fluorescent protein (GFP) is firmly established as a fluorescent reporter for the imaging of specific tissues in zebrafish. The employment of other reporters such as DsRed in transgenic zebrafish has made multicolored labeling experiments possible. To date, several DsRed transgenic lines have been generated for lineage labeling, transplantation assays, and commercial applications. Advances in multicolored labeling experiments will depend on the implementation of newly engineered reporters and fusion proteins, as well as on innovative experiments that exploit the power of direct visualization.
Zhu
Hao
H
Division of Hematology/Oncology, Children's Hospital of Boston, Department of Pediatrics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Zon
Leonard I
LI
D000818:Animals; D030801:Animals, Genetically Modified; D017690:Cell Transplantation / Q000379:methods; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods*; D015027:Zebrafish / Q000235:genetics* / Q000502:physiology; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein; C413663:fluorescent protein 583; C485192:green fluorescent protein, Aequorea victoria