Temporal temperature gradient gel electrophoresis (TTGE) as a tool for the characterization of arbuscular mycorrhizal fungi.
The aim of this study was to assess the feasibility of using temporal temperature gradient electrophoresis (TTGE) of PCR-amplified 18S rDNA fragments of different Glomus species for their detection and characterization. Screening of Glomus clarum, Glomus constrictum, Glomus coronatum, Glomus intraradices, Glomus mosseae and Glomus viscosum by PCR-TGGE revealed that the NS31-AM1 region of the 18S rRNA gene contained insufficient variation to discriminate between them. In contrast, TTGE analysis of the NS31-Glo1 region, which was obtained by nested PCR of the NS31-AM1 amplicon, showed that each species was characterized by a specific TTGE fingerprint. However, isolates of the same species could not be distinguished. The nested PCR-TTGE approach developed allowed identification of the Glomus species colonising the roots of different plant species.
Cornejo
Pablo
P
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, C. Profesor Albareda 1, 18008 Granada, Spain.
Azcón-Aguilar
Concepción
C
Barea
José Miguel
JM
Ferrol
Nuria
N
D004275:DNA, Ribosomal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005658:Fungi / Q000145:classification* / Q000235:genetics; D027523:Lavandula / Q000382:microbiology*; D016533:Mycological Typing Techniques; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D013950:Thymus Gland / Q000382:microbiology*
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S