Characterization of the N-linked glycans of Giardia intestinalis.
This article reports the first rigorous evidence for the existence of N-glycans in Giardia intestinalis, a parasite that is a widespread human pathogen, being a major cause of enteric disease in the world. Excreted/secreted molecules of G. intestinalis are known to stimulate the immune system. Structural strategies based on MALDI and electrospray mass spectrometry were employed to examine the excreted/secreted molecules for their N-glycan content. These revealed that the major oligosaccharides released by peptide N-glycosidase F are complex-type structures and correspond to bi-, and triantennary structures without core (alpha1,6) fucosylation. The major nonreducing epitopes in these complex-type glycans are: Galbeta1-4GlcNAc (LacNAc) and NeuAc alpha2-6Galbeta1-4GlcNAc (sialylated LacNAc).
Morelle
Willy
W
Unité Mixte de Recherche CNRS/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Bâtiment C9, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France. willy.morelle@univ-lille1.fr
Jimenez
Juan-Carlos
JC
Cieniewski-Bernard
Caroline
C
Dei-Cas
Eduardo
E
Michalski
Jean-Claude
JC
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005872:Giardia / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011134:Polysaccharides