An ultraviolet fluorescence-based method for identifying and distinguishing protein crystals.
Intrinsic ultraviolet fluorescence has been investigated as a rapid non-invasive method for identifying and distinguishing protein crystals. An epi-fluorescence microscope, which provides for excitation and viewing of fluorescence from above the sample, and a straight-through geometry, which provides excitation from above and views fluorescence from underneath the sample, were tested with protein and non-protein crystal samples. In both systems the protein crystals were observed to fluoresce brightly, providing a high contrast against background solution fluorescence, thus enabling protein crystals to be identified and distinguished from non-protein crystals.
Judge
Russell A
RA
Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064, USA. russell.judge@abbott.com
Swift
Kerry
K
González
Carlos
C
D019747:Aldose-Ketose Isomerases / Q000737:chemistry; D000818:Animals; D002645:Chickens; D018360:Crystallography, X-Ray / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D009113:Muramidase / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012492:Salts / Q000494:pharmacology; D013696:Temperature; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry; D014466:Ultraviolet Rays
D011506:Proteins; D012492:Salts; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases; D009113:Muramidase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase