title: Sensitive determination of carbohydrates by fluorimetric method with Ce(IV) and sodium triphosphate.
abstract: A new simple and sensitive fluorimetric method for the determination of carbohydrates is described. The method is based on the reaction between carbohydrates and Ce(IV) in the presence of sulfuric acid. All the reductive carbohydrates can be detected indirectly by the fluorescence of Ce(III) produced. The addition of sodium triphate enhances the sensitivity of the method by more than 10-folds. Under optimum conditions, an excellent linear relationship was obtained between the fluorescence intensity and the concentration of carbohydrates. The limits of detection lie in the range of 9.3 x 10(-10) - 1.3 x 10(-9) mol/L. As compared to the normal fluorimetric method, the proposed method is faster and more sensitive.
authors.affiliation: Key Laboratory of Colloid and Interface Chemistry, Department of Chemistry, Shandong University, Ministry of Education, Jinan 250100, People's Republic of China. yjh@sdu.edu.cn
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yang
authors.forename: Xihui
authors.initials: X
authors.lastname: Cao
authors.forename: Changxia
authors.initials: C
authors.lastname: Sun
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002563:Cerium / Q000737:chemistry*; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D011122:Polyphosphates / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D011122:Polyphosphates; D013431:Sulfates; C107379:cerium sulfate; D002563:Cerium; C005692:triphosphoric acid