title: Hydrogen breath test in the diagnosis of lactose malabsorption: accuracy of new versus conventional criteria.
abstract: Increased hydrogen excretion in the breath after carbohydrate ingestion, an expression of carbohydrate malabsorption, represents the pathophysiologic basis of the hydrogen breath test, a simple, noninvasive, reproducible test for the diagnosis of this condition. Few data are available concerning the breath hydrogen response that most accurately identifies carbohydrate malabsorption. In this article we report our application for the first time in clinical practice of 2 recently described, more accurate criteria for the diagnosis of lactose malabsorption (ie, breath hydrogen excretion value > 6 parts per million (ppm) 6 hours after carbohydrate load and a sum greater than 15 ppm for the breath hydrogen values obtained 5, 6, and 7 hours after carbohydrate load). On 3 separate days, we subjected 84 consecutive patients with functional or organic gastrointestinal disease to measurement of hydrogen excretion in the breath after the administration of lactose to test for lactose malabsorption; after the administration of lactulose as a means of ruling out false-negative results resulting from hydrogen-nonproducer status; and after the administration of a nonabsorbable electrolyte solution as a means of ruling out false-positive results caused by the mixing of intestinal content and release of preformed hydrogen trapped in the feces. According to the conventional criterion, 51% of the subjects proved to be lactose malabsorbers. Positive results were obtained with the use of the 6th-hour criterion in 76% of patients, and positive results were found in 80% when the sum of the excretion at hours 5, 6, and 7 was used as the criterion (P < .05 for both comparisons). Ten of 21 and 11 of 25, respectively, showed intolerance symptoms. It was therefore possible for us to diagnose lactose malabsorption in 24% and 27% of patients, respectively, who tested negative according to the conventional criterion. The conventional criterion must be abandoned and these new criteria adopted.
authors.affiliation: Gastroenterology Unit, University of Pavia, Istituto di Ricovero e Cuza a Carattere Scientifico S Matteo Hospital, Pavia, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Di Stefano
authors.forename: Antonio
authors.initials: A
authors.lastname: Missanelli
authors.forename: Emanuela
authors.initials: E
authors.lastname: Miceli
authors.forename: Alessandra
authors.initials: A
authors.lastname: Strocchi
authors.forename: Gino Roberto
authors.initials: GR
authors.lastname: Corazza
mesh_terms: D000328:Adult; D001944:Breath Tests / Q000379:methods*; D004040:Dietary Carbohydrates / Q000493:pharmacokinetics; D005215:Fasting; D005260:Female; D006801:Humans; D006859:Hydrogen / Q000032:analysis / Q000378:metabolism*; D007787:Lactose Intolerance / Q000175:diagnosis* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D004040:Dietary Carbohydrates; D006859:Hydrogen