Advances in surface-enhanced fluorescence.
We report recent achievements in metal-enhanced fluorescence from our laboratory. Several fluorophore systems have been studied on metal particle-coated surfaces and in colloid suspensions. In particular, we describe a distance dependent enhancement on silver island films (SIFs), release of self-quenching of fluorescence near silver particles, and the applications of fluorescence enhancement near metalized surfaces to bioassays. We discuss a number of methods for various shaped silver particle deposition on surfaces.
Lakowicz
Joseph R
JR
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA. lakowicz@cfs.umbi.umd.edu
Geddes
Chris D
CD
Gryczynski
Ignacy
I
Malicka
Joanna
J
Gryczynski
Zygmunt
Z
Aslan
Kadir
K
Lukomska
Joanna
J
Matveeva
Evgenia
E
Zhang
Jian
J
Badugu
Ramachandram
R
Huang
Jun
J
D000465:Algorithms; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry; D004563:Electrochemistry; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry / Q000528:radiation effects; D008670:Metals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D010777:Photochemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
D003102:Colloids; D005456:Fluorescent Dyes; D008670:Metals; D001360:Avidin; D012710:Serum Albumin, Bovine; D012834:Silver; D001710:Biotin; D004247:DNA