title: [Influence of live flora on lactose digestion in male adult lactose-malabsorbers after dairy products intake].
abstract: OBJECTIVE
  To evaluate the effect of live flora on lactose absorption in male adult lactose-malabsorbers.
  
  
  METHODS
  After giving consents, 45 health male adult volunteers were challenged with 20 g lactose in 200 ml water in the morning without breakfast. The expired samples of breath were collected at every 30 min point in 3 hours. 24 persons were chosen as subjects (lactose-malabsorbers) when their expired H2 of any time point was 30 micromol/mol more than the baseline, and divided into 3 groups randomly. The test dairy were yogurt (Y) and heat-treated yogurt (HY), and acidified gelled milk (M), all containing 20 g lactose. The study was double blind, randomized and 3 x 3 latin square design. Three group subjects ingested the 3 different dairy products in 3 different days. The washout time was 7 days. After drinking each dairy in the morning without breakfast, the expired samples at every 30 min were collected during 8 hours test time with no food and no violent exercise. Hydrogen concentration of expired sample was detected by gas chromatography.
  
  
  RESULTS
  The peak hydrogen concentration (Cmax) of yogurt is significant less than heat-treated yogurt and milk (Y 113.2 +/- 70.2, HY 186.5 +/- 96.7, M 174.7 +/- 73.9). The peak time of Cmax (Tmax) of yogurt is significant more than milk (Y 390 +/- 86, M 305 +/- 105). The area under the hydrogen concentration curve (AUC) of yogurt is significant less than the other two dairy products (Y 14,900.1 +/- 12,206.2, HY 27,389.5 +/- 20,429.8, M 25,648.2 +/- 14,064.0).
  
  
  CONCLUSIONS
  The live flora in dairy product could improve lactose digestion in male adult lactose malabsorbers.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Mei
authors.initials: M
authors.lastname: He
authors.forename: Jean-Mechile
authors.initials: JM
authors.lastname: Antoine
authors.forename: Yuexin
authors.initials: Y
authors.lastname: Yang
authors.forename: Jingming
authors.initials: J
authors.lastname: Yang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Men
authors.forename: Hui
authors.initials: H
authors.lastname: Han
mesh_terms: D000328:Adult; D000818:Animals; D019540:Area Under Curve; D004311:Double-Blind Method; D006801:Humans; D006859:Hydrogen / Q000032:analysis; D007778:Lactobacillus; D007785:Lactose / Q000378:metabolism*; D007787:Lactose Intolerance / Q000378:metabolism*; D008297:Male; D008892:Milk; D048249:Streptococcus thermophilus; D015014:Yogurt / Q000382:microbiology*
chemical_list: D006859:Hydrogen; D007785:Lactose