The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes.
Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype can be reversed by injection of Gpr3 RNA into the oocytes. Thus, the GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle and is crucial for the regulation of meiosis.
Mehlmann
Lisa M
LM
Department of Cell Biology, University of Connecticut Health Center (UCHC), Farmington, CT 06032, USA. lmehlmann@neuron.uchc.edu
Saeki
Yoshinaga
Y
Tanaka
Shigeru
S
Brennan
Thomas J
TJ
Evsikov
Alexei V
AV
Pendola
Frank L
FL
Knowles
Barbara B
BB
Eppig
John J
JJ
Jaffe
Laurinda A
LA
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D005260:Female; D006107:Granulosa Cells / Q000502:physiology; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D017403:In Situ Hybridization; D037181:Lectins, C-Type; D008024:Ligands; D007986:Luteinizing Hormone / Q000378:metabolism; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008938:Mitosis; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000502:physiology*; D053675:Versicans
D011508:Chondroitin Sulfate Proteoglycans; C494613:GPR3 protein, mouse; D037181:Lectins, C-Type; D008024:Ligands; D043562:Receptors, G-Protein-Coupled; C508323:Vcan protein, mouse; D053675:Versicans; D012313:RNA; D007986:Luteinizing Hormone; D020962:Heterotrimeric GTP-Binding Proteins; D000262:Adenylyl Cyclases