Production and in vivo effects of chemokines CXCL1-3/KC and CCL2/JE in a model of inflammatory angiogenesis in mice.
OBJECTIVE
Using the murine sponge model, we investigated the temporal relationship between angiogenesis, leukocyte accumulation and endogenous generation of the pro-inflammatory chemokines CXCL1-3/KC and CCL2/JE. Furthermore, the effects of exogenous administration of these chemokines were studied.


METHODS
Angiogenesis in the implants was assessed by measuring the hemoglobin content (vascular index) and leukocyte accumulation quantified by evaluating MPO and NAG enzyme activities.


RESULTS
A progressive increase in hemoglobin content and in enzymatic activities was observed during the whole period. The levels of CXCL1-3/KC and CCL2/JE in the implants peaked at days 7 and 1, respectively. Exogenous administration of CXCL1-3/KC (100 ng/day intra-implant) applied at days 1-3 resulted in increased neovascularization and macrophage accumulation. Intra-implant injections of CCL2/JE (100 ng/day) also resulted in increased angiogenesis and macrophage accumulation.


CONCLUSIONS
These results demonstrated that the chemokines, CXCL1-3/KC and CCL2/JE, are generated within the sponge compartment and that neovascularization and inflammatory cells influx can be modulated by exogenous administration of the chemokines.
Barcelos
L S
LS
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627-Pampulha, 31270-901, Belo Horizonte MG, Brasil.
Talvani
A
A
Teixeira
A S
AS
Cassali
G D
GD
Andrade
S P
SP
Teixeira
M M
MM
D000118:Acetylglucosaminidase / Q000737:chemistry; D000818:Animals; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D054360:Chemokine CXCL1; D018925:Chemokines / Q000378:metabolism; D019743:Chemokines, CXC / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006454:Hemoglobins / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic; D009504:Neutrophils / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors
D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; C475895:Cxcl1 protein, mouse; D006454:Hemoglobins; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D009195:Peroxidase; D000118:Acetylglucosaminidase