Proteomic analysis of the proliferative and secretory phases of the human endometrium: protein identification and differential protein expression.
Proteomic analyses of the proliferative and secretory phases of the human endometrium were carried out to identify proteins and discover differentially expressed proteins using isotope-coded affinity tags, three stages of chromatographic separation and online tandem mass spectrometry (MS/MS). From an initial list of 346 proteins identified by ProICAT, manual inspection of MS/MS spectra and confirmatory searches pared the list down to 119 positively identified proteins. Only five of the proteins showed consistent differential expression. The utility of some of these proteins as indicators of true differential expression in the endometrium is open to discussion. The two proteins with unquestionable differential expressions in the secretory endometrium are: glutamate NMDA receptor subunit zeta 1 precursor and FRAT1. Some of the proteins that show no differential expression have previously been examined in gene-expression studies with similar conclusions.
DeSouza
Leroi
L
Department of Chemistry and Centre for Research in Mass Spectrometry, York University, Toronto M3J 1P3, Ontario, Canada.
Diehl
Georg
G
Yang
Eric C C
EC
Guo
Jingzhong
J
Rodrigues
Mary Joe
MJ
Romaschin
Alexander D
AD
Colgan
Terence J
TJ
Siu
K W Michael
KW
D048868:Adaptor Proteins, Signal Transducing; D002853:Chromatography, Liquid; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D011498:Protein Precursors / Q000096:biosynthesis; D021122:Protein Subunits / Q000096:biosynthesis; D020543:Proteome / Q000096:biosynthesis*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D048868:Adaptor Proteins, Signal Transducing; C491819:FRAT1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011498:Protein Precursors; D021122:Protein Subunits; D020543:Proteome; D011518:Proto-Oncogene Proteins; D016194:Receptors, N-Methyl-D-Aspartate