title: Comparison of the pharmacokinetics of lidamycin in mice determined by two methods.
abstract: OBJECTIVE
  To compare two methods, the total radioactivity assay (RA method) and the radioactivity assay after separation with high performance liquid chromatography (HPLC-RA method).
  
  
  METHODS
  125I-Lidamycin was prepared by Iodogen method and separated by size exclusive high performance liquid chromatography. The pharmacokinetic parameters of lidamycin were assayed by two methods after intravenous injection to mice at the dose of 100 microg x kg(-1), and compared by statistical analysis.
  
  
  RESULTS
  The pharmacokinetic parameters (Vd, T1/2alpha, T1/2beta, K21, K10, K12, AUC and CL) showed significant difference between the two methods (P < 0.05).
  
  
  CONCLUSIONS
  The HPLC-RA method was better than the RA method to determine unchanged 125I-lidamycin.
authors.affiliation: National Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China.
authors.forename: You-ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Quan-sheng
authors.initials: QS
authors.lastname: Li
authors.forename: Yu-rong
authors.initials: YR
authors.lastname: Huang
authors.forename: Chang-xiao
authors.initials: CX
authors.lastname: Liu
mesh_terms: D000617:Aminoglycosides / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000097:blood / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D053281:Enediynes; D005260:Female; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D012680:Sensitivity and Specificity
chemical_list: D000617:Aminoglycosides; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D007457:Iodine Radioisotopes; C058024:C 1027