[Production of DNA-hydrolyzing antibody BV04-01 Fab fragment in methylotrophic yeast Pichia pastoris].
Efficient system for producing the recombinant Fab of DNA-hydrolyzing antibody BV04-01 in metylotrophic yeast Pichia pastoris was developed. Addition of peptides encompassing the Jun-Fos leucine zipper at the C-termini of the antibody chains facilitated the in vivo assembling of the Fab. The yield of secreted functionally active BV04-01 Fab was about 3 mg/L. Catalytic efficiency of supercoiled DNA hydrolysis by the Fab obtained was 1.8 x 10(6) M(-1) min(-1).
Kozyr'
A V
AV
Bobik
T V
TV
Ignatova
A N
AN
Kolesnikov
A V
AV
D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000276:immunology / Q000378:metabolism*; D006868:Hydrolysis; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins / Q000276:immunology
D017931:DNA Primers; D004271:DNA, Fungal; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins