N-linked glycan structures of human lactoferrin produced by transgenic rice.
Human lactoferrin was produced in genetically engineered rice. N-linked glycan structures of recombinant human lactoferrin were determined. The oligosaccharides liberated by hydrazinolysis were labeled with 2-aminopyridine (PA). The PA-labeled glycans were purified by reverse-phase and size-fractionation HPLCs. The structures of these glycans were identified by HPLC, exoglycosidase digestion, and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The glycan structures determined were ManFucXylGlcNAc(2) (3.4%), Man(2)FucGlcNAc(2) (2.1%), Man(3)FucGlcNAc(2) (2.5%), Man(3)FucXylGlcNAc(2) (42.5%), two isomers of Man(2)FucXylGlcNAc(2) (39.1%), Man(3)XylGlcNAc(2) (6.5%), and Man(2)XylGlcNAc(2) (3.9%).
Fujiyama
Kazuhito
K
International Center for Biotechnology, Osaka University 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. fujiyama@icb.osaka-u.ac.jp
Sakai
Yohei
Y
Misaki
Ryo
R
Yanagihara
Itaru
I
Honda
Takeshi
T
Anzai
Hiroyuki
H
Seki
Tatsuji
T
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007781:Lactoferrin / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D011134:Polysaccharides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011134:Polysaccharides; D011994:Recombinant Proteins; D007781:Lactoferrin