title: Molecular analysis of delta-aminolevulinic acid dehydratase (ALAD) gene polymorphism in a Turkish population.
abstract: delta-Aminolevulinic acid dehydratase (ALAD) is a cytosolic enzyme in the heme biosynthetic pathway. ALAD is a polymorphic enzyme showing marked ethnic group differences. In this study, ALAD polymorphism is studied in a Turkish population. Genomic DNA extracted from 230 individuals and polymerase chain reaction (PCR) coupled with the restriction fragment length polymorphism (RFLP) technique were used to identify variants. The frequencies of the alleles ALAD1 and ALAD2 in Turkey were 0.887 and 0.113, respectively. This study provides the first analysis of the allele frequency distribution of the ALAD gene in a Turkish population. The results are compared with other world populations.
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, University of Ankara, Tandogan 06100, Ankara, Turkey. suzen@pharmacy.ankara.edu.tr
authors.forename: Halit Sinan
authors.initials: HS
authors.lastname: Süzen
authors.forename: Yalçin
authors.initials: Y
authors.lastname: Duydu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Aydin
mesh_terms: D000483:Alleles; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D005820:Genetic Testing; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D000623:Porphobilinogen Synthase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014421:Turkey
chemical_list: D000623:Porphobilinogen Synthase