Effect of an avidin-biotin binding system on chondrocyte adhesion, growth and gene expression.
Cell adhesion to synthetic biomaterials is a prerequisite for anchorage cell culture and tissue engineering. The current study investigated utilization of an avidin-biotin binding system in enhancing chondrocyte adhesion to tissue culture polystyrene (TCPS). Biotinylated chondrocytes adhered to avidin-coated TCPS more quickly than untreated chondrocytes to bare TCPS. Also the avidin-biotin binding system enhanced cell initial spreading. However, the effects were only transient. The growth of biotinylated chondrocytes was first decreased during the first 3 days but increased afterwards. The progeny of biotinylated chondrocytes still maintained the ability in expressing cartilage extracellular matrix proteins such as type II collagen, type IX collagen and aggrecan. These results show potential for the application of the avidin-biotin binding system to cell culture and tissue engineering.
Tsai
Wei-Bor
WB
Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan. weibortsai@ntu.edu.tw
Wang
Min-Cheng
MC
D000818:Animals; D001360:Avidin / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D046508:Culture Techniques; D017931:DNA Primers / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D008858:Microscopy, Phase-Contrast; D011137:Polystyrenes / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013997:Time Factors; D023822:Tissue Engineering
D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D017931:DNA Primers; D011137:Polystyrenes; D001360:Avidin; D001710:Biotin