Adrenergic modulation of cytokine release in bone marrow progenitor-derived macrophage following polymicrobial sepsis.
Catecholamines may impact on the pathophysiology of sepsis by attenuating proinflammatory cytokine and augmenting antiinflammatory cytokine production by macrophages. We tested this premise in bone marrow monocyte progenitor-derived macrophages. Polymicrobial sepsis was induced in mice through cecal ligation and puncture. ER-MP 12 monocyte progenitors were isolated and differentiated into macrophages in vitro 72 hr later. Lipopolysaccharide (LPS)-stimulated cytokine production was measured with and without epinephrine, IL-10 and anti-IL-10 antibody. Epinephrine significantly increased IL-10 production, but attenuated TNF-alpha release exclusively through beta2 adrenergic receptors, and is independent of IL-10 production. Together, these results suggest that epinephrine can promote a potent antiinflammatory response in sepsis.
Muthu
Kuzhali
K
Department of Physiology, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Ave, Maywood, IL 60153, USA.
Deng
Jiangping
J
Gamelli
Richard
R
Shankar
Ravi
R
Jones
Stephen B
SB
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001262:Atenolol / Q000494:pharmacology; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004347:Drug Interactions; D004731:Endotoxins / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004837:Epinephrine / Q000378:metabolism* / Q000494:pharmacology; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D007813:Laparotomy / Q000379:methods; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D023461:Myeloid Progenitor Cells / Q000187:drug effects / Q000502:physiology*; D011412:Propanolamines / Q000494:pharmacology; D011897:Random Allocation; D018805:Sepsis / Q000276:immunology / Q000503:physiopathology*
D000319:Adrenergic beta-Antagonists; D000906:Antibodies; D016207:Cytokines; D004731:Endotoxins; D008070:Lipopolysaccharides; D011412:Propanolamines; D016753:Interleukin-10; C026777:ICI 118551; D001262:Atenolol; D004837:Epinephrine