[Effect of truncated PDGF-alpha receptor on cellular cycle and proliferation for vascular cells of pulmonary artery].
OBJECTIVE
To determine the effect of truncated PDGF-alpha receptor on cellular cycle and proliferation of pulmonary artery vascular smooth muscular cells (VSMC).


METHODS
Tissue mass culture was done to get vascular smooth muscular cells of pulmonary artery. Different concentrations of truncated PDGF-alpha receptor and adenoviral recombined body (Ad5CMV-PalphaRtr, ACP) were added into the cultures; the VSMC proliferation curve was observed using MTT test. The changes of VSMC cellular cycle were also observed by flow cytometry.


RESULTS
ACP at mid to high concentrations can restrain the proliferation of VSMC apparently with the peak of cell growth being attenuated or eliminated. The curve presented an ascending tendency only after 7 to 9 days. Affected by mid-concentration ACP, PDGF-BB showed no significant accelerating effect on the proliferation of VSMC. The ratio of G0/G1 in cellular cycle was increased significantly in VSMC culture with ACP in comparison with control. The G0/G1 ratio also showed significant differences among different concentration ACP groups (P < 0.05).


CONCLUSIONS
Mid-to high-concentration ACP is a powerful ihhibitor of cellular proliferation for pulmonary artery VSMC. It can increase the ratio of G0/G1 significantly and the increase seems to be ACP dosage dependent.
Zhou
Tongfu
T
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Liu
Hanmin
H
Zhang
Ping
P
Luo
Chunhua
C
D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011651:Pulmonary Artery / Q000166:cytology; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000502:physiology*; D013552:Swine
D020796:Receptor, Platelet-Derived Growth Factor alpha