Characterization of parvalbumin, the major allergen in Alaska pollack, and comparison with codfish Allergen M.
Increased fish consumption has led to frequent reporting of fish allergy and adverse reactions. Alaska pollack (Theragra chalcogramma) is a globally important commercial fish species, belonging to the Gadidae family. This family of fish also includes cod whose parvalbumin, Allergen M (Gad c 1), has been thoroughly studied and considered as a reference to sensitization in fish allergy. In the present study, parvalbumin from Alaska pollack, designated The c 1, was purified by use of anion exchange chromatography. To demonstrate the homogeneity of the purified protein, reverse phase high performance liquid chromatography was performed and showed two distinct fractions which had similar IgG and IgE binding capacities. Accordingly, cDNA cloning revealed two isotypic parvalbumin transcripts in pollack muscle. Recombinant parvalbumins of pollack exhibited low IgG and IgE binding capacities, in contrast to the native counterparts, which were almost as potent as cod Gad c 1. The allergenicity of The c 1 was assayed by ELISA inhibition, and compared to cod, the concentration required for obtaining 50% ELISA inhibition (C 50%) was only 18% higher for The c 1.
Van Do
Thien
T
Allergy Research Group, Laboratory of Clinical Biochemistry, Department of Medicine, Haukeland University Hospital, University of Bergen, NO-5021 Bergen, Norway. van.do@helse-bergen.no
Hordvik
Ivar
I
Endresen
Curt
C
Elsayed
Said
S
D000485:Allergens / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology; D048388:Gadiformes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D010320:Parvalbumins / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D020539:Sequence Analysis, Protein
C033061:Allergen M protein, codfish; D000485:Allergens; D018076:DNA, Complementary; D029941:Fish Proteins; D007074:Immunoglobulin G; D010320:Parvalbumins; D011994:Recombinant Proteins; D007073:Immunoglobulin E