Cy3B: improving the performance of cyanine dyes.
The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an antibody. This study compared the conjugated emission properties of Cy3B with other commercially available fluorophores emitting at similar wavelengths.
Cooper
Michael
M
Amersham Biosciences, Discovery Systems, The Maynard Centre, Whitchurch, Cardiff, CF14 7YT, United Kingdom. michael.cooper@amersham.com
Ebner
Andreas
A
Briggs
Mark
M
Burrows
Miles
M
Gardner
Nicholas
N
Richardson
Robert
R
West
Richard
R
D001557:Benzenesulfonates / Q000138:chemical synthesis / Q000737:chemistry*; D002232:Carbocyanines / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry
D001557:Benzenesulfonates; D002232:Carbocyanines; C495965:Cy3B N-hydroxysuccinimide ester; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; C089187:cyanine dye 3