A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells.
Human retinal pigment epithelium (HRPE) cells are important in maintaining the normal physiology within the neurosensory retina and photoreceptors. Recently, transplantation of HRPE has become a possible therapeutic approach for retinal degeneration. By negative immunoselection (CD45 and glycophorin A), in this study, we have isolated and cultivated adult human bone marrow stem cells (BMSCs) with multilineage differentiation potential. After a 2- to 4-week culture under chondrogenic, osteogenic, adipogenic, and hepatogenic induction medium, these BMSCs were found to differentiate into cartilage, bone, adipocyte, and hepatocyte-like cells, respectively. We also showed that these BMSCs could differentiate into neural precursor cells (nestin-positive) and mature neurons (MAP-2 and Tuj1-positive) following treatment of neural selection and induction medium for 1 month. Furthermore, the plasticity of BMSCs was confirmed by initiating their differentiation into retinal cells and photoreceptor lineages by co-culturing with HRPE cells. The latter system provides an ex vivo expansion model of culturing photoreceptors for the treatment of retinal degeneration diseases.
Chiou
Shih-Hwa
SH
Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan. schiou@vghtpe.gov.tw
Kao
Chung-Lan
CL
Peng
Chi-Hsien
CH
Chen
Shih-Jen
SJ
Tarng
Yih-Wen
YW
Ku
Hung-Hai
HH
Chen
Yu-Chih
YC
Shyr
Yi-Ming
YM
Liu
Ren-Shyan
RS
Hsu
Chien-Jen
CJ
Yang
De-Ming
DM
Hsu
Wen-Ming
WM
Kuo
Cheng-Deng
CD
Lee
Chen-Hsen
CH
D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D018920:Coculture Techniques; D006801:Humans; D009474:Neurons / Q000502:physiology; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D015220:Calcium Channels