[Application of modified ABTS+ assay method to monitor antioxidant extraction from fruit].
OBJECTIVE
Modify the ABTS method to measure the antioxidant activity of compound. Apply this method to optimize antioxidant extraction from fruit.


METHODS
In microplate, Trolox served as reference. Antioxidant activity was measured according to their ability to scavenge ABTS+ in the reaction mixture. Under different temperatures, solvent solid ratios, solvent concentrations, antioxidants were extracted.


RESULTS
The standard error for measuring Trolox antioxidant activity was under 0.2. The antioxidant activities of fourteen standard samples were consistent with that reported in literatures. Using ethanol as solvent, at about 17 degrees C, for 1 gram of blackcurrant 20 ml of 67% ethanol was appropriate. While using sodium metabisulphite as solvent, at about 30 degrees C, for 1 gram of blackcurrant 20 ml of 300 mg/L metabisulphite would be appropriate.


CONCLUSIONS
Modified ABTS+ method for measuring antioxidant activity was simple and feasible. This method could be applied to monitor antioxidant extraction from fruit and high throughput screening for antioxidant.
Han
Guangliang
G
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Li
Cuimei
C
Cacace
Eduardo
E
Mazza
G
G
Yang
Xiaoguang
X
D000975:Antioxidants / Q000032:analysis*; D052160:Benzothiazoles; D000431:Ethanol; D005638:Fruit / Q000737:chemistry*; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods; D013447:Sulfites; D013451:Sulfonic Acids; D013696:Temperature
D000975:Antioxidants; D052160:Benzothiazoles; D012997:Solvents; D013447:Sulfites; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D000431:Ethanol; C030981:metabisulfite