Conjugation of antibodies to alkaline phosphatase.
Alkaline phosphatase is coupled to immunoglobin G antibody in a one-step procedure using the homobifunctional reagent glutaraldehyde, which reacts with amino groups in the two proteins. The procedure is simple to perform and requires minimal equipment.
Wisdom
G Brian
GB
School of Biology and Biochemistry, The Queen's University, Belfast, Northern Ireland, UK.
D000469:Alkaline Phosphatase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D005976:Glutaral / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*
D007074:Immunoglobulin G; D000469:Alkaline Phosphatase; D005976:Glutaral