title: Phylogenetic relationships of rhizobia based on citrate synthase gene sequences.
abstract: Partial nucleotide sequences of the citrate synthase (gltA) gene from different rhizobia genera were determined. Tree topologies based on this housekeeping gene were similar to that obtained using 16S rRNA sequences. However gltA appeared to be more reliable at determining phylogenetic relationships of closely related taxa. We propose gltA sequences as an additional tool to be used in molecular phylogenetic studies.
authors.affiliation: Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. ismael@cifn.unam.mx
authors.forename: Ismael
authors.initials: I
authors.lastname: Hernández-Lucas
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Rogel-Hernández
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Segovia
authors.forename: Keilor
authors.initials: K
authors.lastname: Rojas-Jiménez
authors.forename: Esperanza
authors.initials: E
authors.lastname: Martínez-Romero
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D002950:Citrate (si)-Synthase / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002950:Citrate (si)-Synthase