title: NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly.
abstract: The detailed conformational analysis of a single molecule of the tetraacyl biosynthetic precursor-type lipid A and its characteristic supramolecular assembly in aqueous SDS-micelles are described. Regular molecular arrangements were observed by detailed analysis of the NMR spectra of synthetically pure specimens, including regiospecifically 13C-labeled ones. NMR analysis of a biologically inactive precursor-type analogue with four shorter acyl chains demonstrated its conformational flexibility, indicating the importance of hydrophobic interactions for maintaining the conformation of such molecules.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Oikawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shintaku
authors.forename: Naohiro
authors.initials: N
authors.lastname: Fukuda
authors.forename: Harald
authors.initials: H
authors.lastname: Sekljic
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Fukase
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yoshizaki
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukase
authors.forename: Shoichi
authors.initials: S
authors.lastname: Kusumoto
mesh_terms: D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D008050:Lipid A / Q000032:analysis* / Q000096:biosynthesis; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
chemical_list: D002241:Carbohydrates; D008050:Lipid A