Yellow fever virus isolated from a fatal post vaccination event: an experimental comparative study with the 17DD vaccine strain in the Syrian hamster (Mesocricetus auratus).
In order to investigate the pathogenicity of the virus strain GOI 4191 that was isolated from a fatal adverse event after yellow fever virus (YFV) vaccination, an experimental assay using hamsters (Mesocricetus auratus) as animal model and YFV 17DD vaccine strain as virus reference was accomplished. The two virus strains were inoculated by intracerebral, intrahepatic and subcutaneous routes. The levels of viremia, antibody response, and aminotransferases were determined in sera; while virus, antigen and histopathological changes were determined in the viscera. No viremia was detected for either strain following infection; the immune response was demonstrated to be more effective to strain GOI 4191; and no significant aminotransferase levels alterations were detected. Strain GOI 4191 was recovered only from the brain of animals inoculated by the IC route. Viral antigens were detected in liver and brain by immunohistochemical assay. Histothological changes in the viscera were characterized by inflammatory infiltrate, hepatocellular necrosis, and viral encephalitis. Histological alterations and detection of viral antigen were observed in the liver of animals inoculated by the intrahepatic route. These findings were similar for both strains used in the experiment; however, significant differences were observed from those results previously reported for wild type YFV strains.
Rodrigues
Sueli Guerreiro
SG
Instituto Evandro Chagas, Belém, PA, Brasil. suelirodrigues@iec.pa.gov.br
da Rosa
Amélia Paes de Andrade Travassos
AP
Galler
Ricardo
R
Barros
Vera Lúcia Reis de Souza
VL
Vieira
Conceição de Maria Almeida
Cde M
da Rosa
Jorge Fernando Soares Travassos
JF
Azevedo
Raimunda do Socorro da Silva
Rdo S
Nunes
Márcio Roberto Teixeira
MR
da Rosa
Elizabeth Salbé Travassos
ES
Vasconcelos
Pedro Fernando da Costa
PF
D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001921:Brain / Q000473:pathology / Q000821:virology; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology / Q000821:virology; D008297:Male; D008647:Mesocricetus; D010641:Phenotype; D014709:Vero Cells; D015004:Yellow Fever / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D022341:Yellow Fever Vaccine; D015005:Yellow fever virus / Q000276:immunology / Q000472:pathogenicity*
D000914:Antibodies, Viral; D022341:Yellow Fever Vaccine; D000410:Alanine Transaminase