Malfunction of vascular control in lifestyle-related diseases: distribution of adrenomedullin-containing perivascular nerves and its alteration in hypertension.
The distribution and characteristics of adrenomedullin (AM)-containing perivascular nerves in the rat mesenteric artery were investigated using immunohistochemical techniques. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia of mesenteric arteries, which were densely innervated by calcitonin gene-related peptide (CGRP)- and neuropeptide Y (NPY)-LI fibers. AM-LI, CGRP-LI, and NPY-LI fibers were abolished by cold storage denervation. Capsaicin pretreatment abolished AM-LI and NPY-LI fibers but not NPY-LI fibers. NPY-LI fibers but not AM-LI and CGRP-LI fibers disappeared after treatment with 6-hydroxydopamine. There were many AM-LI positive cells in the dorsal root ganglia, where AM mRNA was detected. In a double immunofluorescence study, AM-LI was found in CGRP-LI fibers, although some fibers contained AM-LI alone. The density of AM-LI fibers was lower in SHR than in WKY mesenteric arteries. These results suggest that the mesenteric artery is innervated by AM-containing perivascular nerves and AM may have a neurotransmitter role in the regulation of vascular tone.
Hobara
Narumi
N
Department of Clinical Pharmaceutical Science, Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Nakamura
Arisa
A
Goda
Mitsuhiro
M
Kawasaki
Hiromu
H
D053607:Adrenomedullin; D000367:Age Factors; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D006801:Humans; D006973:Hypertension / Q000378:metabolism*; D007150:Immunohistochemistry; D008638:Mesenteric Arteries / Q000294:innervation*; D009478:Neuropeptide Y / Q000032:analysis; D010455:Peptides / Q000032:analysis / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D009478:Neuropeptide Y; D010455:Peptides; D053607:Adrenomedullin; D015740:Calcitonin Gene-Related Peptide