Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS.
The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine were studied by HPLC on two kinds of chiral columns: (S,S)-Whelk-O 1 and ChiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on ChiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was extensively studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61 on (S,S)-Whelk-O 1 column with hexane-ethanol (97:3, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on ChiraSpher column with hexane-ethanol-THF-diethylamine (92:3:5:0.1, v/v/v/v) as the mobile phase. LC-MS methods were developed to identify the isomer peaks.
Pan
Chun-xiu
CX
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Xu
Xiu-zhu
XZ
He
Hong-mei
HM
Cai
Xiao-jun
XJ
Zhang
Xue-jun
XJ
D000081:Acetamides / Q000032:analysis* / Q000737:chemistry*; D002072:Butylene Glycols / Q000032:analysis* / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*; D010880:Piperidines / Q000032:analysis* / Q000737:chemistry*; D011725:Pyridines / Q000032:analysis* / Q000737:chemistry*
D000081:Acetamides; D002072:Butylene Glycols; D010880:Piperidines; D011725:Pyridines; C042544:penitricin C; C076948:lafutidine