title: A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58.
abstract: OBJECTIVE
  The aim of this study was to analyse the spread of the bla(OXA-58) gene as a source of carbapenem resistance in Acinetobacter baumannii in the burns unit of a university hospital in Toulouse in 2003-2004.
  
  
  METHODS
  Six carbapenem-resistant A. baumannii isolates from six patients, and a carbapenem-resistant environmental A. baumannii isolate were collected in the burns unit of the Rangueil hospital (Toulouse). Susceptibility tests were carried out by disc diffusion and agar dilution methods. The detection of the bla(OXA-58) gene was conducted by PCR followed by sequence analysis. Plasmids were extracted and hybridized with a probe specific for bla(OXA-58). DNA fingerprints were obtained by pulsed-field gel electrophoresis of ApaI- or SmaI-digested chromosomal DNA of the tested strains.
  
  
  RESULTS
  The multidrug-resistant clinical isolates had a similar 30 kb plasmid that encoded the carbapenem-hydrolysing beta-lactamase OXA-58. These isolates were clonally related. The unrelated environmental carbapenem-resistant A. baumannii isolate had a similar bla(OXA-58)-carrying plasmid, suggesting spread of this gene.
  
  
  CONCLUSIONS
  A novel oxacillinase was the source of carbapenem resistance in the A. baumannii isolates. Its gene was plasmid-, but not integron-borne.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Héritier
authors.forename: Anne
authors.initials: A
authors.lastname: Dubouix
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Nicole
authors.initials: N
authors.lastname: Marty
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002053:Burn Units; D015780:Carbapenems / Q000378:metabolism / Q000494:pharmacology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D005602:France / Q000453:epidemiology; D006785:Hospitals, University; D006801:Humans; D015378:Imipenem / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015780:Carbapenems; D015378:Imipenem; D001618:beta-Lactamases; C108449:oxacillinase