title: The telomere repeat binding protein Trf1 interacts with the spindle checkpoint protein Mad1 and Nek2 mitotic kinase.
abstract: The mitotic spindle checkpoint arrests cells in metaphase until all kinetochores are correctly attached to spindle fibres. In a search for new components of the spindle checkpoint, based on protein interaction, we have identified mouse Telomeric Repeat Binding Factor 1 (Trf1) as a protein that interacts directly with the spindle checkpoint protein Mad1 and the mitotic kinase Nek2. Trf1 has a role in the regulation of telomere length, but has also been implicated in cell cycle regulation. These interactions now provide a direct link between telomere length regulation and the control of mitotic progression.
authors.affiliation: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
authors.forename: Graham
authors.initials: G
authors.lastname: Prime
authors.forename: David
authors.initials: D
authors.lastname: Markie
mesh_terms: D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation; D018386:Kinetochores / Q000502:physiology; D008677:Metaphase / Q000235:genetics / Q000502:physiology; D051379:Mice; D008938:Mitosis / Q000235:genetics / Q000502:physiology; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000235:genetics / Q000502:physiology; D016615:Telomere / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D035321:Telomeric Repeat Binding Protein 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C499479:Mad protein, mouse; D012097:Repressor Proteins; D035321:Telomeric Repeat Binding Protein 1; D000072379:NIMA-Related Kinases; C490317:Nek2 protein, mouse; D017346:Protein Serine-Threonine Kinases