Isolation of genomic DNA using magnetic cobalt ferrite and silica particles.
Adsorption separation techniques as an alternative to laborious traditional methods (e.g., based on phenol extraction procedure) have been applied for DNA purification. In this work we used two types of particles: silica and cobalt ferrite (unmodified or modified with a reagent containing weakly basic aminoethyl groups, aminophenyl groups, or alginic acid). DNA from chicken erythrocytes and DNA isolated from bacteria Lactococcus lactis were used for testing of adsorption/desorption properties of particles. The cobalt ferrite particles modified with different reagents were used for isolation of PCR-ready bacterial DNA from different dairy products.
Prodelalová
Jana
J
Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 60200 Brno, Czech Republic.
Rittich
Bohuslav
B
Spanová
Alena
A
Petrová
Katerina
K
Benes
Milan J
MJ
D000327:Adsorption; D000818:Animals; D002645:Chickens; D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005290:Ferric Compounds / Q000737:chemistry*; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*
D005290:Ferric Compounds; C001215:ferrite; D003035:Cobalt; D012822:Silicon Dioxide; D004247:DNA