[Sequence variability in Borna disease virus ORF II from human beings].
40 clones from five psychiatric patients and three healthy blood donors in whom Borna disease virus (BDV) ORF II fragments in PBMC were previously proved by nested RT-PCR were sequenced and compared with BDV/MDCK. There were eleven point mutations in all clones compared with reported HE80-1, but no difference between human and BDV/MDCK clones. We found specific sequence variability at nt 1701 in ten clones from two psychiatric disorder patients (T-->C) and at nt 1696 in fifteen clones from three healthy blood donors (A-->T). This result strongly suggests there are some special gene divergence in BDV derived from human beings.
Xie
P
P
Institute of Neurology and Psychiatry, Chongqing University of Medical Sciences, China 400016.
Iwata
Y
Y
Takahashi
H
H
D001483:Base Sequence; D001890:Borna Disease / Q000821:virology*; D001891:Borna disease virus / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D006801:Humans; D001523:Mental Disorders / Q000821:virology; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D016415:Sequence Alignment