[Nernst potential as a driving force of the fast transmembrane diffusion (flip-flop) of the anionic natural phospholipid phosphatidylethanol].
A unique rapid flip-flop of the naturally occurring negatively charged phospholipid phosphatidylethanol in sonicated phosphatidylcholine vesicles was studied using 13C NMR and phosphatidylethanol 13C-labeled at the CH2 ethyl moiety. The transbilayer diffusion of phosphatidylethanol was observed in response to the formation of Nernst potential (t 1/2 < 7 min), indicating that phosphatidylethanol transfer occurs in the anionic form. The application of the fluorescent potential-sensitive dye di-4-ANEPPS showed that the phosphatidylethanol movement across the membrane was accompanied by a decrease in the Nernst potential. In biological membranes, a facile spontaneous diffusion of phosphatidylethanol implies the lack of high asymmetry of its distribution, even if phosphatidylethanol is synthesized exclusively on one side of the membrane.
Viktorov
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D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D020404:Glycerophospholipids / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity; D008564:Membrane Potentials; D010713:Phosphatidylcholines / Q000737:chemistry; D014634:Valinomycin / Q000737:chemistry
D005456:Fluorescent Dyes; D020404:Glycerophospholipids; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; C051521:phosphatidylethanol; D014634:Valinomycin; C017251:1,2-oleoylphosphatidylcholine