Modulation of the innate immune response to HSV-1 following acute administration of morphine: role of hypothalamo-pituitary-adrenal axis.
A single injection of morphine significantly increased interferon (IFN)-beta and IFN-alpha mRNA in spleens from herpes simplex virus (HSV)-1 infected male Balb/cByJ mice. However, significant suppression of IFN-gamma and interleukin (IL)-12 production was observed in spleens from morphine-treated mice. Pretreatment with RU486 blocked morphine-induced increases in IFN-beta, and reversed the suppression of IFN-gamma. However, RU486 did not restore IFN-alpha or IL-12. The results suggest that a single exposure to morphine 4 h prior to infection can significantly alter innate immune responses to a viral pathogen, and that these effects are partially mediated by glucocorticoids.
Sheridan
Patricia A
PA
Interdepartmental Program in Neuroscience, 575 Elmwood Ave., University of Rochester, Rochester, NY 14621, USA. patricia_sheridan@med.unc.edu
Moynihan
Jan A
JA
D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D020805:Central Nervous System Viral Diseases; D003345:Corticosterone / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000276:immunology* / Q000472:pathogenicity; D006727:Hormone Antagonists / Q000494:pharmacology; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology; D007372:Interferons / Q000145:classification / Q000235:genetics / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015735:Mifepristone / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology*; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000276:immunology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000821:virology; D013997:Time Factors
D006727:Hormone Antagonists; D009294:Narcotics; D012333:RNA, Messenger; D018664:Interleukin-12; D015735:Mifepristone; D011092:Polyethylene Glycols; D009020:Morphine; D007372:Interferons; D003345:Corticosterone