[Gene therapy for ovarian cancer by adenovirus-mediated transduction of cytosine deaminase gene in vitro].
OBJECTIVE
To study the value of adenovirus mediated expression of the Escherichia coli cytosine deaminase (CD) gene with 5-fluorocytosine (5-FC) in the gene therapy of ovarian cancer cells.


METHODS
Ovarian cancer cells were transduced CD gene by adenovirus vector and exposed to varying concentration of 5-FC. After 5 days of incubation, MTT assays were performed to measure the cell viability.


RESULTS
It was shown that tumor cells transduced with CD gene had a high sensitivity to 5-FC. Mixed cellular assay revealed that CD-postive cells had a neighbor cell killing effect on CD-negative cells when exposed to 5-FC. 20% CD positive cells killed more than 80% mixed cells.


CONCLUSIONS
CD/5-FC gene therapy approach is an effective anti-tumor strategy in the treatment of ovarian cancer.
Li
Qingli
Q
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Peng
Zhilan
Z
Wang
He
H
Yao
Yuan
Y
Zhang
Jiawen
J
D000256:Adenoviridae / Q000235:genetics; D000964:Antimetabolites, Antineoplastic / Q000378:metabolism / Q000494:pharmacology; D045744:Cell Line, Tumor; D003001:Cloning, Molecular / Q000379:methods; D043525:Cytosine Deaminase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005472:Fluorouracil / Q000378:metabolism / Q000494:pharmacology*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D014161:Transduction, Genetic
D000964:Antimetabolites, Antineoplastic; D043525:Cytosine Deaminase; D005472:Fluorouracil