[Effect of ginsenoside Rg1 on expression of p21, cyclin E and CDK2 in the process of cell senescence].
OBJECTIVE
To explore the possible role of p21, cyclin E and cyclin-dependent kinase 2 (CDK2) in the protection of ginsenoside Rg1 against tert-butylhydroperoxide (t-BHP)-induced senescence in WI-38 cells.


METHODS
The cellular ultrastructure, cytometric assay and beta-galactosidase (beta-gal) cytochemistry staining were used to evaluate cell senescence. The levels of of p21, cyclin E and CDK2 protein were detected by Western blot.


RESULTS
Pretreatment with Rg1 significantly attenuated t-BHP-induced senescence in WI-38 cells. Simultaneously, compared with cells treated with t-BHP alone, Rg1 pretreatment markedly decreased the level of p21 protein and increased the levels of CDK2 and cyclin E.


CONCLUSIONS
p21, cyclin E and CDK2 may be involved in the process of ginsenoside Rg1 protection against t-BHP-induced senescence in WI-38 cells.
Zhao
Chao-hui
CH
The Affiliated Union Hospital, Fujian Medical University, Fujian Institute of Geriatrics, Fuzhou 350001, China.
Chen
Xiao-chun
XC
Jin
Jian-sheng
JS
Zhu
Yuan-gui
YG
Shi
Guang-bin
GB
Zeng
Yu-qi
YQ
Li
Yong-kun
YK
Peng
Xu
X
D042846:CDC2-CDC28 Kinases / Q000378:metabolism*; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects*; D019927:Cyclin E / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors
D019927:Cyclin E; D036145:Ginsenosides; D020122:tert-Butylhydroperoxide; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); C035054:ginsenoside Rg1