Silent hemoglobin variants and determination of HbA(1c) with the high-resolution program of the HPLC HA-8160 hemoglobin analyzer.
OBJECTIVE
Evaluation of HbA1c determination with an automated ion-exchange high-performance liquid chromatography (HPLC) method in patients with clinically silent hemoglobin (Hb) variants.


METHODS
HbA1c values were determined with the Arkray HA-8160 ion-exchange HPLC using the high-resolution, 4.2-min beta-thalassemia screening mode in patients with silent hemoglobin (Hb) variants, namely, Hb Graz, Hb Sherwood Forest, Hb O Padova, and HbD.


RESULTS
All of these hemoglobin variants caused additional peaks in the chromatograms, without HbA1c results in patients with Hb Graz and Hb Sherwood Forest, and demonstrated extra peaks with HbA1c results that were clinically too low for patients with Hb O Padova and in the patient with HbD.


CONCLUSIONS
The development of this automated HPLC method modification with high-resolution beta-thalassemia screening mode aids identification of interference due to some clinically silent Hb variants in HbA(1c) determination.
Schnedl
Wolfgang J
WJ
Department of Internal Medicine, Medical University of Graz, A-8036 Graz, Austria. wolfgang.schnedl@meduni-graz.at
Lahousen
Theresa
T
Wallner
Sandra J
SJ
Krause
Robert
R
Lipp
Rainer W
RW
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003922:Diabetes Mellitus, Type 1 / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D014644:Genetic Variation; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000235:genetics*; D006455:Hemoglobins, Abnormal / Q000032:analysis / Q000235:genetics; D006801:Humans
D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C079327:hemoglobin Graz; C032001:hemoglobin D; C079325:hemoglobin Sherwood Forest; C038278:hemoglobin O Padova