title: Validation of the postimplantation rat whole-embryo culture test in the international ECVAM validation study on three in vitro embryotoxicity tests.
abstract: A detailed report is presented on the performance of the postimplantation rat whole-embryo culture (WEC) test in a European Centre for the Validation of Alternative Methods (ECVAM)-sponsored formal validation study on three in vitro tests for embryotoxicity. Twenty coded test chemicals, classified as non-embryotoxic, weakly embryotoxic or strongly embryotoxic on the basis of their in vivo effects in animals and/or humans, were tested in four laboratories. The outcome showed that the WEC test can be considered to be a scientifically validated test, which is ready for consideration for use in assessing the embryotoxic potentials of chemicals for regulatory purposes.
authors.affiliation: National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
authors.forename: Aldert H
authors.initials: AH
authors.lastname: Piersma
authors.forename: Elke
authors.initials: E
authors.lastname: Genschow
authors.forename: Aart
authors.initials: A
authors.lastname: Verhoef
authors.forename: Marielle Q I
authors.initials: MQ
authors.lastname: Spanjersberg
authors.forename: Nigel A
authors.initials: NA
authors.lastname: Brown
authors.forename: Madeleine
authors.initials: M
authors.lastname: Brady
authors.forename: Angie
authors.initials: A
authors.lastname: Burns
authors.forename: Nicole
authors.initials: N
authors.lastname: Clemann
authors.forename: Andrea
authors.initials: A
authors.lastname: Seiler
authors.forename: Horst
authors.initials: H
authors.lastname: Spielmann
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D016002:Discriminant Analysis; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects*; D047108:Embryonic Development; D005060:Europe; D007391:International Cooperation; D004364:Pharmaceutical Preparations / Q000145:classification; D011786:Quality Control; D051381:Rats; D015203:Reproducibility of Results; D018675:Toxicity Tests / Q000379:methods
chemical_list: D004364:Pharmaceutical Preparations