Impact of isolation method on the antioxidant activity of rapeseed meal phenolics.
Rapeseed meal is the byproduct of the rapeseed deoiling process. Among oilseed plants, rapeseed contains the greatest amount of phenolic compounds. In this study, the rapeseed phenolics were isolated with aqueous methanol, aqueous ethanol, hot water, and enzymatically with ferulic acid esterase. These isolates were tested for radical scavenging and for liposome and low-density lipoprotein (LDL) model systems. The radical scavenging activities of all isolates were >60% at a concentration of 1.5 mg/mL. In the liposome model system the formation of hexanal was inhibited by all rapeseed meal isolates by >90% and the formation of conjugated diene hydroperoxides by >80% (8.4 microg/mL concentration). All rapeseed meal isolates also inhibited oxidation of LDL particles by >90% inhibition (4.2 microg/mL concentration). Isolation of rapeseed meal phenolics with either water or enzyme is a very suitable method devoid of organic solvents. Thus, rapeseed meal phenolics constitute an interesting source for food and cosmetic applications with antioxidant effect.
Vuorela
Satu
S
Department of Applied Chemistry and Microbiology, Division of Food Chemistry, University of Helsinki, P.O. Box 27, 00014 University of Helsinki, Finland. satu.vuorela@helsinki.fi
Meyer
Anne S
AS
Heinonen
Marina
M
D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D029741:Brassica rapa / Q000737:chemistry*; D002265:Carboxylic Ester Hydrolases; D000431:Ethanol; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology*; D014867:Water
D000975:Antioxidants; D010636:Phenols; D014867:Water; D000431:Ethanol; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D000432:Methanol