[Establishment of transgenic mice of hepatitis B virus X gene (adr subtype) mediated by spermatogonial cells].
OBJECTIVE
To test the possibility of using spermatogonial cells as vector in the establishment of HBV X gene transgenic mice.


METHODS
Eukaryoti expression vector pcDNA3-X of hepatitis B virus X gene was constructed. The recombinant pcDNA3-X packed by liposome was injected into testicle tissue of male C57BL/6N mice using microvolume injector. Six weeks after the first injection, the mice were crossed with female mice. Polymerase chain reaction and immunohistochemistry were applied to identify the viral DNA(HBx gene) harbored n liver tissue.


RESULTS
The sequence of the X gene is consistent with that reported in literature. The X gene can be expressed in Hela cells. The positive rate in daughter mice was 6.25%.


CONCLUSIONS
It is possible to use spermatogonial cells as vector to establish HBx transgenic mice.
Hu
Huozhen
H
Department of Cell Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Dan
D
Li
Xiaoyu
X
Yao
Yucheng
Y
Hu
Yiping
Y
D000818:Animals; D005260:Female; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
D011994:Recombinant Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein