Identification of genes controlled by the manganese response regulator, ManR, in the cyanobacterium, Anabaena sp. PCC 7120.
A computational search was carried out to identify additional binding sites for the manganese response regulator, ManR, in the genome of Anabaena sp. PCC 7120. This approach predicted ManR binding sites: the promoter regions of the genes of all3575-alr3576 and the gene of alr5134 from Anabaena sp. PCC 7120. Electrophoretic mobility shift assays confirmed that the ManR of Anabaena sp. PCC 7120 specifically bound to the promoter regions of all3575-alr3576 and alr5134.
Huang
Wei
W
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China.
Wu
Qing-Yu
QY
D017033:Anabaena / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001665:Binding Sites; D002874:Chromosome Mapping / Q000379:methods*; D004586:Electrophoresis; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020539:Sequence Analysis, Protein / Q000379:methods*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins