[Establishment of two-dimensional electrophoresis of uterine leiomyoma for the proteome analysis].
To establish and optimize the two-dimensional electrophoresis (2-DE) of uterine leiomyoma for the proteome analysis.



Run immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.



The 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.



With optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.
Zhu
Xue-qiong
XQ
Women's Hospital, College of Medicine, Zhejiang University, Hangzhou, 310006, China.
Shi
Yi-fu
YF
Chen
Huai-zeng
HZ
Chen
Qi
Q
D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000737:chemistry*; D009215:Myometrium / Q000737:chemistry; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D014594:Uterine Neoplasms / Q000737:chemistry*
D009363:Neoplasm Proteins; D020543:Proteome