title: Growth-inhibitory effect of antibacterial self-etching primer on mutans streptococci obtained from arrested carious lesions.
abstract: BACKGROUND
  An antibacterial self-etching primer has been developed to inhibit bacterial attachment and plaque accumulation on the tooth surfaces. The purpose of this study was to evaluate the growth-inhibitory effect of an antibacterial self-etching primer on mutans streptococci obtained from arrested carious lesions.
  
  
  METHODS
  Softened dentin specimens were obtained from arrested carious lesions with an excavator or a round steel bur. The effect of a commercial antibacterial self-etching primer and a commercial self-etching primer on the total streptococci on Mitis-Salivarius (MS) agar plates and on Streptococcus mutans on MS agar plates containing 0.02 M bacitracin (MSB) was evaluated. Specimens with no primer were used as controls. The number of colonies of both total streptococci and S. mutans were compared statistically with two-way analysis of variance and Fisher's Protected Least Squares Differences (PLSD) test (p < .05).
  
  
  RESULTS
  The number of colonies of total streptococci (CFU/mL) for the two methods (excavator and round steel bur) were as follows: for the control, 5.0 x 10(6) and 5.0 x 10(6); for the self-etching primer, 1.0 x 10(5) and 1.0 x 10(5); and for the antibacterial self-etching primer, 0 and 0. The number of colonies of S. mutans for the two methods (excavator and round steel bur) were as follows: for the control, 5.0 x 10(6) and 1.8 x 10(5); for the self-etching primer, 3.5 x 10(4) and 5.0 x 10(3); and for the antibacterial self-etching primer, 0 and 0. Regardless of the method of softened dentin removal, the antibacterial effect was significantly higher for the group that had antibacterial self-etching primer compared with that of the control group and the group that had commercial self-etching primer (p < .05).
  
  
  CONCLUSIONS
  The antibacterial self-etching primer showed a high level of antibacterial activity against mutans streptococci obtained from the arrested carious lesions.
authors.affiliation: Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. kitasako.ope@tmd.ac.jp
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kitasako
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Senpuku
authors.forename: Richard M
authors.initials: RM
authors.lastname: Foxton
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hanada
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001422:Bacterial Adhesion / Q000187:drug effects; D003731:Dental Caries / Q000628:therapy; D045662:Dental Etching / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents