title: Construction of a eukaryotic expression plasmid of Humanin.
abstract: OBJECTIVE
  To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.
  
  
  METHODS
  The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.
  
  
  RESULTS
  Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence.
  
  
  CONCLUSIONS
  A eukaryotic expression plasmid of Humanin was successfully constructed.
authors.forename: Ben-yan
authors.initials: BY
authors.lastname: Luo
authors.forename: Xiang-ming
authors.initials: XM
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011994:Recombinant Proteins; C427211:humanin