title: [Expression of human telomere repeat binding factor 1 (TRF1) in acute leukemia cells and its correlation with telomerase activities].
abstract: To study the expression of human telomere repeat binding factor 1 (TRF1) to investigate the correlation of telomerase activity with acute leukemia.
  
  
  
  Leukemic cells were collected from 30 cases of acute leukemia. Realtime quantitative PCR with fluorescence probe hybridization was used to measure expression of TRF1 and hTERT mRNA in leukemic cells.
  
  
  
  TRF1 mRNA expression was 0.0126 (0.0127-0.0546) in acute non-lymphocytic leukemia (ANLL), which was lower than that in normal mononuclear cells [0.0457 (0.00839-0.262), P<0.001], but its expression in acute lymphoblastic leukemia (ALL) cells [0.0745 (1.92 x 10(-6)-0.193)] had no significant difference compared with that in normal mononuclear cells. TRF1 expression in ANLL cells was significantly lower than that in ALL cells (P=0.001). The expressions of TRF1 mRNA in AL cells and normal mononuclear cells had no significant correlation with expression of hTERT mRNA (r=-0.173, P=0.207).
  
  
  
  The expression of TRF1 is lower in ANLL cells, which indicates TRF1 may have some effect on telomerase activity by regulating telomere length in ANLL cells.
authors.affiliation: The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Lai
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Zhu
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Lan
authors.forename: Li-dan
authors.initials: LD
authors.lastname: Tang
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.forename: Ya-min
authors.initials: YM
authors.lastname: Tan
authors.forename: Mao-fang
authors.initials: MF
authors.lastname: Lin
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019098:Telomerase / Q000378:metabolism*; D035321:Telomeric Repeat Binding Protein 1 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D035321:Telomeric Repeat Binding Protein 1; D019098:Telomerase