Schizophyllan-folate conjugate as a new non-cytotoxic and cancer-targeted antisense carrier.
Schizophyllan having folate-appendages was synthesized from native schizophyllan through NaIO(4)-oxidation and the subsequent reductive amination in aqueous ammonia followed by amido-coupling with folic acid. The resulting folate-appended schizophyllan can form stable complex with poly(dA), show specific affinity toward folate binding protein, and mediate effective antisense activity in cancer cells.
Hasegawa
Teruaki
T
Department of Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Fujisawa
Tomohisa
T
Haraguchi
Shuichi
S
Numata
Munenori
M
Karinaga
Ryouji
R
Kimura
Taro
T
Okumura
Shiro
S
Sakurai
Kazuo
K
Shinkai
Seiji
S
D001483:Base Sequence; D002352:Carrier Proteins / Q000138:chemical synthesis / Q000494:pharmacology; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007624:KB Cells; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011956:Receptors, Cell Surface; D012566:Sizofiran / Q000737:chemistry* / Q000494:pharmacology; D013997:Time Factors
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D016376:Oligonucleotides, Antisense; D011956:Receptors, Cell Surface; D012566:Sizofiran; D005492:Folic Acid