Positional signaling mediated by a receptor-like kinase in Arabidopsis.
The position-dependent specification of root epidermal cells in Arabidopsis provides an elegant paradigm for cell patterning during development. Here, we describe a new gene, SCRAMBLED (SCM), required for cells to appropriately interpret their location within the developing root epidermis. SCM encodes a receptor-like kinase protein with a predicted extracellular domain of six leucine-rich repeats and an intracellular serine-threonine kinase domain. SCM regulates the expression of the GLABRA2, CAPRICE, WEREWOLF, and ENHANCER OF GLABRA3 transcription factor genes that define the cell fates. Further, the SCM gene is expressed throughout the developing root. Therefore, SCM likely enables developing epidermal cells to detect positional cues and establish an appropriate cell-type pattern.
Kwak
Su-Hwan
SH
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA.
Shen
Ronglai
R
Schiefelbein
John
J
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002455:Cell Division; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D057927:Hydrophobic and Hydrophilic Interactions; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009154:Mutation; D019441:Plant Epidermis / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D018517:Plant Roots / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D018749:RNA, Plant; D014157:Transcription Factors; D020794:Receptor Protein-Tyrosine Kinases; C503317:SUB protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases