[Molecular mechanism of wendan tang in prevention of lipid metabolism disorder in adult rats].
OBJECTIVE
To elucidate the molecular mechanism of Wendan Tang in prevention of lipid metabolism disorder in adult rats.


METHODS
On the basis of hyperlipidemia rat models, triglycerides (TG), total cholesterol (TC) in serum, activities of lipase (LA), lipoprotein lipase (LPL), hepatic lipase (HL) in liver, parts of hemogram and hepatic LDLR mRNA levels were investigated 21 days after the feeding of atherogenic diet.


RESULTS
Wendan Tang significantly reduced the serum TG, TC and increased the activity of LPL and LA, but caused no chang in HL. The result of RT-PCR test showed that high fat and high cholesterol feeding could significantly induce the reduction of LDLR mRNA levels, while Wendan Tang could increase hepatic LDLR density.


CONCLUSIONS
Wendan Tang can prevent disorder of lipid metabolism by regulating TC, TG, LDL-c through upregaulation of LDLR transcription level and improving antioxidant ability.
Chun
Zhe
Z
Sichuan University, Chengdu 610064, Sichuan, China.
Li
Jia-lan
JL
Cheng
Dong-hui
DH
Luo
Xia
X
Yang
Zhi-rong
ZR
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006949:Hyperlipidemias / Q000097:blood* / Q000378:metabolism / Q000517:prevention & control; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011973:Receptors, LDL / Q000096:biosynthesis* / Q000235:genetics
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D011973:Receptors, LDL