The long-range organization of a native photosynthetic membrane.
Photosynthesis relies on the delicate interplay between a specific set of membrane-bound pigment-protein complexes that harvest and transport solar energy, execute charge separation, and conserve the energy. We have investigated the organization of the light-harvesting (LH) and reaction-center (RC) complexes in native bacterial photosynthetic membranes of the purple bacterium Rhodobacter sphaeroides by using polarized light spectroscopy, linear dichroism (LD) on oriented membranes. These LD measurements show that in native membranes, which contain LH2 as the major energy absorber, the RC-LH1-PufX complexes are highly organized in a way similar to that which we found previously for a mutant lacking LH2. The relative contribution of LH1 and LH2 light-harvesting complexes to the LD spectrum shows that LH2 preferentially resides in highly curved parts of the membrane. Combining the spectroscopic data with our recent atomic force microscopy (AFM) results, we propose an organization for this photosynthetic membrane that features domains containing linear arrays of RC-LH1-PufX complexes interspersed with LH2 complexes and some LH2 found in separate domains. The study described here allows the simultaneous assessment of both global and local structural information on the organization of intact, untreated membranes.
Frese
Raoul N
RN
Biophysics, Faculty of Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1081, Amsterdam 1081 HV, The Netherlands. raoul@nat.vu.nl
Siebert
C Alistair
CA
Niederman
Robert A
RA
Hunter
C Neil
CN
Otto
Cees
C
van Grondelle
Rienk
R
D001426:Bacterial Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000502:physiology; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000502:physiology; D017434:Protein Structure, Tertiary; D012242:Rhodobacter sphaeroides / Q000378:metabolism; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins