Expression of GDNF in spinal cord injury and its repression by ONO-1714.
ONO-1714 is a newly developed specific inhibitor for inducible nitric oxide synthase (iNOS). We have shown that ONO-1714 has some neuroprotective effects. In this report, we investigated the effects of ONO-1714 in injured spinal cords, and analyzed the expression of glial cell-line-derived neurotrophic factor (GDNF) after injury. Male Sprague-Dawley rats were subjected to contusive spinal cord injury and administrated 0.1 mg/kg ONO-1714. The injured spinal cords were isolated at appropriate time points and GDNF mRNA was determined by semi-quantitative RT-PCR. GDNF-positive cells were also counted after immunohistochemical stainings. ONO-1714 diminished the early stage production of GDNF after injury as well as it reduced the production of nitric oxide produced by iNOS and apoptotic cells.
Nakashima
Shojiro
S
Department of Orthopaedic Surgery, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya, 466-8550, Japan. shojiro@med.nagoya-u.ac.jp
Matsuyama
Yukihiro
Y
Yu
Yimin
Y
Katayama
Yoshito
Y
Ito
Zenya
Z
Ishiguro
Naoki
N
D000578:Amidines / Q000627:therapeutic use*; D000818:Animals; D017931:DNA Primers; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006574:Heterocyclic Compounds, 2-Ring / Q000627:therapeutic use*; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors / Q000235:genetics*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013116:Spinal Cord / Q000473:pathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
C407360:7-chloro-3-imino-5-methyl-2-azabicyclo(4.1.0)heptane; D000578:Amidines; D017931:DNA Primers; D004791:Enzyme Inhibitors; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006574:Heterocyclic Compounds, 2-Ring; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat