In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings.
An in-house mycobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.
Símboli
Norberto
N
INEI-ANLIS "Carlos G. Malbrán," Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.
Takiff
Howard
H
McNerney
Ruth
R
López
Beatriz
B
Martin
Anandi
A
Palomino
Juan Carlos
JC
Barrera
Lucía
L
Ritacco
Viviana
V
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009160:Mycobacteriophages / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development / Q000821:virology; D011933:Reagent Kits, Diagnostic; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
D000904:Antibiotics, Antitubercular; D011933:Reagent Kits, Diagnostic; D012293:Rifampin