The role of MET1 in RNA-directed de novo and maintenance methylation of CG dinucleotides.
A genetic screen for mutants defective in RNA-directed DNA methylation and transcriptional silencing of the constitutive nopaline synthase (NOS) promoter in Arabidopsis identified two independent mutations in the gene encoding the DNA methyltransferase MET1. Both mutant alleles are disrupted structurally in the MET1 catalytic domain, suggesting that they are complete loss of function alleles. Experiments designed to test the effect of a met1 mutation on both RNA-directed de novo and maintenance methylation of the target NOS promoter revealed in each case approximately wild type levels of non-CG methylation together with significant reductions of CG methylation. These results confirm a requirement for MET1 to maintain CG methylation induced by RNA. In addition, the failure to establish full CG methylation in met1 mutants, despite normal RNA-directed de novo methylation of Cs in other sequence contexts, indicates that MET1 is required for full de novo methylation of CG dinucleotides. We discuss MET1 as a site-specific DNA methyltransferase that is able to maintain CG methylation during DNA replication and contribute to CG de novo methylation in response to RNA signals.
Aufsatz
Werner
W
Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, UZAII, Althanstrasse 14/2D-541, Austria.
Mette
M Florian
MF
Matzke
Antonius J M
AJ
Matzke
Marjori
M
D000483:Alleles; D000594:Amino Acid Oxidoreductases / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D018899:CpG Islands / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D019175:DNA Methylation; D008957:Models, Genetic; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*
D018749:RNA, Plant; D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase; D004248:DNA (Cytosine-5-)-Methyltransferases