Human muscle precursor cell regeneration in the mouse host is enhanced by growth factors.
The aim of this study was to optimize human muscle formation in vivo from implanted human muscle precursor cells. We transplanted donor muscle precursor cells (MPCs) prepared from postnatal or fetal human muscle into immunodeficient host mice and showed that irradiation of host muscle significantly enhanced muscle formation by donor cells. The amount of donor muscle formed in cryodamaged host muscle was increased by exposure of donor cells to growth factors before their implantation into injured host muscle. Insulin-like growth factor type I (IGF-I) significantly increased the amount of muscle formed by postnatal human muscle cells, but not by fetal human MPCs. However, treatment of fetal muscle cells with IGF-I, in combination with basic fibroblast growth factor and plasmin, significantly increased the amount of donor muscle formed. In vivo, human MPCs formed mosaic human-mouse muscle fibers, in which each human myonucleus was associated with a zone of human sarcolemmal protein spectrin.
Brimah
K
K
Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College, London W12 ONN, United Kingdom.
Ehrhardt
J
J
Mouly
V
V
Butler-Browne
G S
GS
Partridge
T A
TA
Morgan
J E
JE
D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D034904:Lamin Type A / Q000096:biosynthesis; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D012038:Regeneration; D013049:Spectrin / Q000378:metabolism; D013997:Time Factors
D006133:Growth Substances; D034904:Lamin Type A; C440308:lamin C; D016222:Fibroblast Growth Factor 2; D013049:Spectrin; D007334:Insulin-Like Growth Factor I