title: [Establishment of transgenic mice of hepatitis B virus X gene (adr subtype) mediated by spermatogonial cells].
abstract: OBJECTIVE
  To test the possibility of using spermatogonial cells as vector in the establishment of HBV X gene transgenic mice.
  
  
  METHODS
  Eukaryoti expression vector pcDNA3-X of hepatitis B virus X gene was constructed. The recombinant pcDNA3-X packed by liposome was injected into testicle tissue of male C57BL/6N mice using microvolume injector. Six weeks after the first injection, the mice were crossed with female mice. Polymerase chain reaction and immunohistochemistry were applied to identify the viral DNA(HBx gene) harbored n liver tissue.
  
  
  RESULTS
  The sequence of the X gene is consistent with that reported in literature. The X gene can be expressed in Hela cells. The positive rate in daughter mice was 6.25%.
  
  
  CONCLUSIONS
  It is possible to use spermatogonial cells as vector to establish HBx transgenic mice.
authors.affiliation: Department of Cell Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Huozhen
authors.initials: H
authors.lastname: Hu
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Yao
authors.forename: Yiping
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D005260:Female; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D011994:Recombinant Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein