Cell-mediated cytotoxicity of murine cytomegalovirus-infected target cells allows for release of residual infectious virus.
Although cell-mediated cytotoxicity effectively kills target virus-infected cells, no careful consideration has been given to the fate of infectious progeny virus contained within target cells following the cytolytic event. To address this issue with respect to murine cytomegalovirus (MCMV) pathogenesis, we developed a rapid semiquantitative assay for infectious MCMV based on expression of beta-galactosidase using a LacZ-expressing recombinant MCMV. Simultaneous use of this assay in combination with a modified cell-mediated cytotoxicity assay revealed that cytotoxicity of MCMV-infected target cells mediated by MCMV-immune splenic cells does not lead to inactivation of intracellular infectious virus.
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R D
RD
Department of Ophthalmology, Harvey and Bernice Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA. drichard@nsu.nova.edu
Cousins
S W
SW
D000818:Animals; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D003602:Cytotoxicity, Immunologic; D051379:Mice; D011994:Recombinant Proteins / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D011994:Recombinant Proteins; D001616:beta-Galactosidase