title: Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray.
abstract: Transcriptional regulation in response to hyperosmotic, high-salinity and oxidative stress, and abscisic acid (ABA) treatment in Arabidopsis suspension-cultured cell line T87 was investigated with a cDNA microarray containing 7000 independent full-length Arabidopsis cDNAs. The transcripts of 102, 11, 84 and 73 genes were increased more than 5-fold within 5h after treatment with 0.5M mannitol, 0.1M NaCl, 50 microM ABA and 10mM H2O2, respectively. On the other hand, the transcripts of 44, 57, 25 and 34 genes were down-regulated to less than one-third within 5h after treatment with 0.5M mannitol, 0.1M NaCl, 50 microM ABA and 10mM H2O2, respectively. Venn diagram analysis revealed 11 genes were induced significantly by mannitol, NaCl, and ABA, indicating crosstalk among these signaling pathways. Comparison of the genes induced by each stress revealed that 32%, 17% and 33% of mannitol-, NaCl- and ABA-inducible genes were also induced by H2O2, indicating the crosstalk between the signaling pathways for osmotic stress and oxidative stress. Although the expression profiles revealed that the T87 cells had most of the regulatory systems seen in Arabidopsis seedlings, the T87 cells did not have one of ABA-dependent signaling pathways.
authors.affiliation: Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba , 305-0074, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Junko
authors.initials: J
authors.lastname: Ishida
authors.forename: Masakazu
authors.initials: M
authors.lastname: Satou
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.forename: Asako
authors.initials: A
authors.lastname: Kamiya
authors.forename: Maiko
authors.initials: M
authors.lastname: Nakajima
authors.forename: Akiko
authors.initials: A
authors.lastname: Enju
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D015152:Blotting, Northern; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006982:Hypertonic Solutions; D008353:Mannitol / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009997:Osmotic Pressure; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D018076:DNA, Complementary; D006982:Hypertonic Solutions; D012333:RNA, Messenger; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid; D006861:Hydrogen Peroxide