Modulation of flowering responses in different Nicotiana varieties.
We have identified and characterized a FLOWERING PROMOTING FACTOR 1 ( FPF1 ) gene from tobacco ( NtFPF1 ). Over-expression of NtFPF1 leads to early flowering in the day-neutral tobacco Nicotiana tabacum cv. Hicks, and under inductive photoperiods also in the short-day Nicotiana tabacum cv. Hicks Maryland Mammoth ( MM ) tobacco and the long-day plant Nicotiana sylvestris . N. sylvestris wild-type plants remained in the rosette stage and never flowered under non-inductive short-days, whereas 35S:: NtFPF1 transgenic plants bolted but did not flower. However, if treated with gibberellins, transgenic N. sylvestris plants flowered much faster under non-inductive short days than corresponding wild type plants, indicating an additive effect of gibberellins and the NtFPF1 protein in flowering time control. The day-neutral wild type cv. Hicks and the short-day cv. Hicks MM plants exhibit an initial rosette stage, both under short- and long-days. In the transgenic lines, this rosette stage was completely abolished. Wild-type plants of cv. Hicks MM never flowered under long days; however, all transgenic lines over-expressing NtFPF1 flowered under this otherwise non-inductive photoperiod.
Smykal
Petr
P
Swiss Federal Institute of Technology Zürich (ETH), Institute of Plant Sciences, Universitaetstrasse 2, CH-8092 Zürich, Switzerland.
Gleissner
Roland
R
Corbesier
Laurent
L
Apel
Klaus
K
Melzer
Siegbert
S
D000595:Amino Acid Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D035264:Flowers / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D018744:DNA, Plant; D005875:Gibberellins; D010940:Plant Proteins; D018749:RNA, Plant