title: Encephalomyocarditis (EMC) virus-induced sialodacryoadenitis in mice.
abstract: The mode of occurrence of the D variant of encephalomyocarditis (EMC-D) virus-induced acute sialodacryoadenitis was investigated using three strains of mice differing in their sensitivity to EMC-D virus-induced diabetes (C57BL/6: resistant; BALB/c: moderately sensitive; DBA/2: highly sensitive). Mice were intranasally inoculated with high (10(5) PFU/mouse) or low dose (10(2) PFU/mouse) of EMC-D virus. Although there were individual differences, the blood virus titer generally reached the peak earlier in the high-dose group than in the low-dose group. Signals of viral RNA and histopathological changes were seen in parotid glands and intraorbital and extraorbital lachrymal glands. In these glands, signals of viral RNA and histopathological changes were detected only in acinar cells and initial lesions were characterized by pyknosis of acinar cells. Coagulative necrosis with interstitial inflammatory cell infiltration developed later in parotid glands of BALB/c mice of the high-dose group and in intraorbital and extraorbital lachrymal glands of all groups except for C57BL/6 mice of the low-dose group. Such changes were not observed in epithelial cells of the ductal system. The present results indicate that EMC-D virus shows clear tissue and cell tropism within the salivary and lachrymal glands, probably due to the distribution of receptors for EMC virus.
authors.affiliation: Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ohguchi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Chiyo
authors.initials: C
authors.lastname: Doi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Kunio
authors.initials: K
authors.lastname: Doi
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000473:pathology / Q000821:virology*; D003607:Dacryocystitis / Q000473:pathology / Q000821:virology*; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D004686:Encephalomyocarditis virus / Q000502:physiology*; D017403:In Situ Hybridization; D007765:Lacrimal Apparatus / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009336:Necrosis; D010306:Parotid Gland / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D012793:Sialadenitis / Q000473:pathology / Q000821:virology*; D013045:Species Specificity; D014766:Viremia
chemical_list: D012367:RNA, Viral