[Extraction and characterization of total DNA from Dendrobium].
OBJECTIVE
To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR.


METHODS
the electrophoresis of total DNA, UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods.


RESULTS
The DNA concentration and yields were different, which greatly influenced the result of RAPD-PCR.


CONCLUSIONS
The higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated.
Peng
Rui
R
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China. wangyhl1971@yahoo.com
Song
Hong-yuan
HY
Li
Quan-sen
QS
Wang
Yu
Y
D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D018744:DNA, Plant / Q000302:isolation & purification*; D031667:Dendrobium / Q000145:classification / Q000235:genetics*; D004586:Electrophoresis; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D011786:Quality Control; D019105:Random Amplified Polymorphic DNA Technique; D013056:Spectrophotometry, Ultraviolet
D002593:Cetrimonium Compounds; D018744:DNA, Plant; D000077286:Cetrimonium