Identification of a new peptide deformylase gene from enterococcus faecium and establishment of a new screening model targeted on PDF for novel antibiotics.
OBJECTIVE
To identify a new peptide deformylase (PDF) gene (Genebank Accession AY238515) from Enterococcus faecium and to establish a new screening model targeted on PDF.


METHODS
A new PDF gene was identified by BLAST analysis and PCR and was subsequently over-expressed in the prokaryotic expression host E. coli B121(DE3). Over-expressed protein was purified for enzymatic assay by metal affinity chromatography and a new screening model was established for novel antibiotics.


RESULTS
A new PDF gene of Enterococcus faecium was identified successfully. Ten positive samples were picked up from 8000 compound library and the microbial fermentation broth samples.


CONCLUSIONS
A new PDF of gene Enterococcus faecium was first identified and the model had a high efficacy. Positive samples screened may be antibacterial agents of broad spectrum.
Tang
Xian-Bing
XB
The National Key Laboratory for Screening New Microbial Drugs, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, China. tanghuoxian@yahoo.com.cn
Si
Shu-Yi
SY
Zhang
Yue-Qin
YQ
D000581:Amidohydrolases / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D003001:Cloning, Molecular; D004353:Drug Evaluation, Preclinical; D016984:Enterococcus faecium / Q000201:enzymology* / Q000235:genetics*; D005450:Fluorescamine; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D013696:Temperature
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D005450:Fluorescamine; D000581:Amidohydrolases; C084507:peptide deformylase