Binding of a monoclonal antibody positively correlates with bioactivity of the F4 fimbrial adhesin FaeG associated with post-weaning diarrhoea in piglets.
Piglets are susceptible to F4 (K88)+ enterotoxigenic Escherichia coli (ETEC)-induced neonatal and post-weaning diarrhoea. The F4 fimbriae are composed of some minor subunits and the major subunit FaeG that also constitutes the adhesin. Parenteral vaccination of sows with an F4-containing vaccine protects the suckling piglets against neonatal F4+ ETEC-induced diarrhoea, but no commercial (mucosal) vaccine exists against F4+ ETEC-induced weaning diarrhoea. To develop a vaccine, a bioactive F4-receptor (F4R) binding FaeG molecule is required that binds to the F4R following oral immunization and induces a FaeG-specific immune response. The present study reports the altered binding of the FaeG-specific monoclonal antibody IMM01 with bioactive versus non-bioactive F4 fimbrial adhesin FaeG. The correlation of altered IMM01 binding with altered FaeG bioactivity permits the use of an IMM01-based ELISA as a fast, specific and sensitive in vitro selection for potent F4 or (recombinant) FaeG antigen formulations, useful in an F4+ ETEC vaccine.
Verdonck
F
F
Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Frank.Verdonck@UGent.be
Snoeck
V
V
Goddeeris
B M
BM
Cox
E
E
D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000833:Animals, Suckling / Q000276:immunology / Q000382:microbiology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D001422:Bacterial Adhesion / Q000276:immunology; D003967:Diarrhea / Q000276:immunology* / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology; D014886:Weaning
D018830:Adhesins, Escherichia coli; D000911:Antibodies, Monoclonal; D022361:Escherichia coli Vaccines; C494388:FaeG protein, E coli; D011994:Recombinant Proteins