title: The novel protein PTPIP51 exhibits tissue- and cell-specific expression.
abstract: The expression patterns of both mRNA and protein of the novel protein tyrosine phosphatase interacting protein 51 (PTPIP51) were studied in various organs by in situ hybridization, immunoblotting, and immunocytochemistry. The protein was found in all mammalian species investigated: guinea pig, rat, mouse, pig, and human. The presence of the protein was, however, restricted to specific organs. High levels of PTPIP51 were found in epidermis and seminiferous epithelium. The expression appears to be associated with distinct stages of differentiation. While basal cells in the epidermis and spermatogonia showed no perceptible amount of PTPIP51, keratinocytes of suprabasal layers and differentiating first-order spermatocytes up to spermatids exhibited high expression. In skeletal muscle, the presence of PTPIP51 was restricted to fibers of the fast twitch type. In surface epithelia containing ciliated cells, the protein was associated with the microtubular structures responsible for ciliary movement. Furthermore, specific structures of the central nervous system, for example, neurons of the hippocampal region, ganglion cells of the autonomic nervous system, and axons of the peripheral nervous system showed a distinct staining pattern with the antibody to PTPIP51. Our data suggest that PTPIP51 might be involved in the regulation of cellular processes associated with differentiation, movement, or cytoskeletal organization.
authors.affiliation: Justus-Liebig-University Giessen, Institute of Anatomy and Cell Biology, Aulweg 123, 35385 Giessen, Germany. Albrecht.T.Stenzinger@anatomie.med.uni-giessen.de
authors.forename: Albrecht
authors.initials: A
authors.lastname: Stenzinger
authors.forename: Tobias
authors.initials: T
authors.lastname: Kajosch
authors.forename: Claudia
authors.initials: C
authors.lastname: Tag
authors.forename: Alexandra
authors.initials: A
authors.lastname: Porsche
authors.forename: Inka
authors.initials: I
authors.lastname: Welte
authors.forename: Hans Werner
authors.initials: HW
authors.lastname: Hofer
authors.forename: Klaus
authors.initials: K
authors.lastname: Steger
authors.forename: Monika
authors.initials: M
authors.lastname: Wimmer
mesh_terms: D000818:Animals; D000906:Antibodies; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D019169:Jurkat Cells; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology; D009475:Neurons, Afferent / Q000166:cytology; D009928:Organ Specificity; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000166:cytology; D013045:Species Specificity; D013552:Swine; D013737:Testis / Q000166:cytology
chemical_list: D000906:Antibodies; D011506:Proteins; D017027:Protein Tyrosine Phosphatases