Galectin-1 supports survival of naive T cells without promoting cell proliferation.
Naive T cells do not proliferate but remain alive in vivo. In contrast, naive T cells rapidly die in an in vitro culture, suggesting that some factors that are present at the sites of naive T cell circulation in vivo but missing in the bovine serum-containing culture medium, are necessary for their survival. The present study was designed to search for such factors. By functional screening of the cDNA library from murine lymph node-derived stromal cells (LNS) that effectively support the survival of naive T cells, we found that nascent polypeptide-associated complex (alpha-NAC) promoted T cell survival. A conditioned medium derived from culture supernatant of Cos7 cells transfected with alpha-NAC gene supported T cell survival, indicating that alpha-NAC induced production of soluble factor(s) that were secreted into the medium. By examining the products that were cloned from a functional screening of the cDNA library from alpha-NAC-transfected NIH3T3 cells but were not detected in that from control vector-transfected cells, galectin-1 was found as a soluble factor in the conditioned medium of the LNS. Our study demonstrates the novel role of galectin-1 as a soluble factor that functions to maintain naive T cell survival without inducing cell proliferation.
Endharti
Agustina Tri
AT
Department of Immunology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Zhou
Yan Wen
YW
Nakashima
Izumi
I
Suzuki
Haruhiko
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned; D018076:DNA, Complementary / Q000235:genetics; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D018832:Molecular Chaperones; D041681:NIH 3T3 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D014162:Transfection; D051020:bcl-X Protein
C494812:Bcl2l1 protein, mouse; D017077:Culture Media, Conditioned; D018076:DNA, Complementary; D037483:Galectin 1; D018832:Molecular Chaperones; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D015534:Trans-Activators; D051020:bcl-X Protein; C096099:nascent-polypeptide-associated complex