title: Upregulation of mRNA expression of vascular endothelial growth factor and its receptors by exogenous human chorionic gonadotropin in cultured oviduct mucosal cells.
abstract: The mucosal cells were isolated from the ampullary regions of 20 human oviducts and cultured with or without hCG in five different concentrations (1-100 ng/mL). As analyzed by the semiquantitative reverse-transcriptase polymerase chain reaction, hCG treatment significantly increased mRNA expression of vascular endothelial growth factor and its receptor flt-1 in the cultured mucosal cells in a dose-dependent manner but had no effect on the expression of another receptor, KDR.
authors.affiliation: Department of Obstetrics and Gynecology, The Prince of Wales Hospital, Shatin, Hong Kong, SAR, China. lampomui@cuhk.edu.hk
authors.forename: Po Mui
authors.initials: PM
authors.lastname: Lam
authors.forename: Christine
authors.initials: C
authors.lastname: Briton-Jones
authors.forename: Che Kwok
authors.initials: CK
authors.lastname: Cheung
authors.forename: Lai Ping
authors.initials: LP
authors.lastname: Cheung
authors.forename: Christopher
authors.initials: C
authors.lastname: Haines
mesh_terms: D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005187:Fallopian Tubes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D009092:Mucous Membrane / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1