Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes.
Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.
Nakanishi
Jun
J
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Kikuchi
Yukiko
Y
Takarada
Tohru
T
Nakayama
Hidekazu
H
Yamaguchi
Kazuo
K
Maeda
Mizuo
M
D000818:Animals; D019556:COS Cells; D002448:Cell Adhesion; D002522:Chlorocebus aethiops; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D041681:NIH 3T3 Cells; D017646:Organosilicon Compounds / Q000737:chemistry*; D010777:Photochemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
C496659:1-(2-nitrophenyl)ethyl-5-trichlorosilylpentanoate; D005353:Fibronectins; D017646:Organosilicon Compounds; D012710:Serum Albumin, Bovine