A novel human gene (WDR25) encoding a 7-WD40-containing protein maps on 14q32.
Members of the large family of WD-repeat proteins are involved in diverse functions such as RNA-procession, signal transduction, vesicular trafficking, cytoskeletal assembly, and cell cycle control. By large-scale-sequencing analysis of a human fetal brain cDNA library, we isolated a novel human cDNA encoding a 7-WD40-repeat protein. This cDNA is 2004 bp in length and it codes for a 544-amino-acid protein. We term it human WD40-repeat-containing gene 25 (WDR25) and this gene shows significant similarity with human pre-mRNA splicing factor 17. The WDR25 gene is mapped to chromosome 14q32 and contains seven exons. RT-PCR analysis shows that the WDR25 gene is widely expressed in human tissues and the expression levers in heart, muscle, testis, ovary, uterus, and prostate are relatively high.
Jin
Feng
F
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Dai
Jianfeng
J
Ji
Chaoneng
C
Gu
Shaohua
S
Wu
Maoqing
M
Qian
Ji
J
Xie
Yi
Y
Mao
Yumin
Y
D020816:Amino Acid Motifs / Q000235:genetics; D018797:Cell Cycle Proteins; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C112200:CDC40 protein, human; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D011506:Proteins; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; C120702:WDR3 protein, human