[DNA damage and apoptosis in L-02 cells induced by chlorination by-product MX of drinking water].
OBJECTIVE
To study DNA damage and apoptosis induced by chlorination by-product MX of drinking water.


METHODS
Single cell gel electrophoresis (SCGE) and flow cytometry were used to detect DNA damage and apoptosis in human fetal hepatocytes (L-02) treated with MX.


RESULTS
DNA strand breaks were found to increase in L-02 cells treated with MX in a dose-response manner. On treatment levels of 100 and 300 micromol/L, MX led to significant increase of DNA damage in comparison to the solvent controls (DMSO) (P < 0.05, and P < 0.01 respectively). Statistically significant increase of L-02 cell apoptosis were observed in all MX treated groups in comparison to the solvent controls (DMSO) (P < 0.001).


CONCLUSIONS
Chlorination by-product MX of drinking water could induce obviously DNA damage and apoptosis in L-02 cells.
Wu
Jianjun
J
Institute of Chemical Carcinogenesis of Guangzhou Medical College, Guangzhou 510182, China.
Liu
Ailin
A
Zou
Yaling
Y
Zhou
Lihong
L
Lu
Wenqing
W
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005663:Furans / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014881:Water Supply
D005663:Furans; D014874:Water Pollutants, Chemical; C054121:3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone