[Establishment and identification of stable transfection of CHO and COS7 cells with TIF3cDNA].
OBJECTIVE
To establish and identify CHO and COS7 cell lines transfected with TIF3cDNA.


METHODS
CHO and COS7 cell lines transfected with TIF3cDNA were established with plasmid (pcDNA3.1/V5-His-TOPO) as the vector, using calcium phosphate inductive transfection techniques and G418 cell selection protocols. Expression of the transfected gene was analyzed and identified using western blotting analysis. The non-transfected group and vector-transfected group were designed to compare with the target gene-transfected group.


RESULTS
3 out of 7 CHO cell lines transfected with TIF3cDNA expressed stably high levels of TIF3 encoded protein. Similarly, 2 out of 4 COS7 cell lines had significant over-expression of TIF3 encoded protein. Conclusion Three CHO and two COS7 cell lines transfected with TIF3cDNA have been successfully established. The cell lines are of great value for the future exploration of Cd compounds related oncogenens and for the studies of the other biological functions of the compounds.
Lei
Yixiong
Y
Department of Preventive Medicine, Guangzhou Medical College, Guangzhou 512182, China.
Pius
Joseph
J
Liu
Yuqing
Y
Tong-man
Ong
O
D000818:Animals; D016466:CHO Cells / Q000166:cytology; D019556:COS Cells / Q000166:cytology; D019256:Cadmium Chloride / Q000633:toxicity; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D039665:Prokaryotic Initiation Factor-3 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D018076:DNA, Complementary; D039665:Prokaryotic Initiation Factor-3; D012333:RNA, Messenger; D019256:Cadmium Chloride