title: Gene expression analysis of peroxisome proliferators- and phenytoin-induced hepatotoxicity using cDNA microarray.
abstract: The recent DNA microarray technology enables us to understand a large number of gene expression profiling. The technology has potential possibility to comprehend mechanism of multiple genes were related to compounds which have toxicity in biological system. So, the toxicogenomics through this technology may be very powerful for understanding the effect of unknown toxic mechanisms in biological system. We have studied that the effect of compounds related to hepatotoxin in vivo system using DNA microarray and classified chemicals which have been well characterized. We have studied three compounds; 2 peroxisome proliferators: Clofibrate (ethyl-p-chlorophenoxyisobutyrate), gemfibrozil (5-2[2,5-dimethyl-phenoxy]2-2-dimethyl-pentanonic), and an antiepileptic drug: phenytoin (5,5-diphenylhydantoin). Male Sprague-Dawely VAF(+) albino rats of 5-6 weeks old were treated with each compound for 24 hr and 2 weeks. 4.8 K cDNA microarray in house has been used for gene expression profiling. We found that the clustering of gene expression had similarity like as the toxic phenotype of compounds.
authors.affiliation: Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd., South Korea.
authors.forename: Jin-Wook
authors.initials: JW
authors.lastname: Jung
authors.forename: Joon-Suk
authors.initials: JS
authors.lastname: Park
authors.forename: Jae-Woong
authors.initials: JW
authors.lastname: Hwang
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
authors.forename: Yong-Soon
authors.initials: YS
authors.lastname: Lee
authors.forename: Bog-Soo
authors.initials: BS
authors.lastname: Song
authors.forename: Gyoung-Jae
authors.initials: GJ
authors.lastname: Lee
authors.forename: Chan-Dong
authors.initials: CD
authors.lastname: Yeo
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Kang
authors.forename: Wan-Seon
authors.initials: WS
authors.lastname: Lee
authors.forename: Ki-Seon
authors.initials: KS
authors.lastname: Jeon
authors.forename: Chan-Hwi
authors.initials: CH
authors.lastname: Um
authors.forename: Yang-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Moon-Ju
authors.initials: MJ
authors.lastname: Oh
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Youn
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D002994:Clofibrate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D015248:Gemfibrozil / Q000633:toxicity*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020675:Peroxisomes / Q000187:drug effects / Q000378:metabolism; D010672:Phenytoin / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000927:Anticonvulsants; D010672:Phenytoin; D002994:Clofibrate; D015248:Gemfibrozil