Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate.
Adventitious roots of ginseng were treated with methyl jasmonate (MJ) up to 150 microM and cultured for 40 days. Up to 100 microM MJ inhibited the root growth but increase ginsenoside accumulation. In a two-stage bioreactor culture, total ginsenosides, after elicitation with 100 microM MJ peaked after 10 days at 48 mg g(-1) dry wt and then dropped sharply. Of the two groups of ginsenosides (Rb and Rg), higher amounts of Rb accumulated in the adventitious roots.
Kim
Yun-Soo
YS
Korea Ginseng Institute, Chung-Ang University, Ansung-shi Kyungii-do, 456-756, Korea.
Hahn
Eun-Joo
EJ
Murthy
Hosakatte Niranjana
HN
Paek
Kee-Yoeup
KY
D000085:Acetates / Q000008:administration & dosage*; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000008:administration & dosage*; D036145:Ginsenosides / Q000096:biosynthesis*; D054883:Oxylipins; D005894:Panax / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*
D000085:Acetates; D003517:Cyclopentanes; D036145:Ginsenosides; D054883:Oxylipins; C072239:methyl jasmonate