In vivo evidence for a regulatory role of the kinase activity of the linotte/derailed receptor tyrosine kinase, a Drosophila Ryk ortholog.
The RYK subfamily of receptor tyrosine kinases is characterised by unusual, but highly conserved, amino acid substitutions in the kinase domain. The linotte/derailed gene encodes a Drosophila RYK subfamily member involved in embryonic and adult central nervous system development. Previous studies have shown that the kinase activity of this receptor is not required in vivo for its embryonic function. In this study, we have investigated the role of the cytoplasmic domain and the kinase activity of the linotte/derailed receptor tyrosine kinase in adult brain development. Our results indicate that these domains are not essential for adult brain development but they are required for the proper regulation of the activity of this receptor. This sheds light on a regulatory role for the kinase activity of a RYK subfamily member.
Taillebourg
Emmanuel
E
Institut de Génétique Humaine, CNRS/UPR 1142, 141 rue de la Cardonille, 34396, Montpellier cedex 5, France.
Moreau-Fauvarque
Caroline
C
Delaval
Katia
K
Dura
Jean-Maurice
JM
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology / Q000254:growth & development; D017931:DNA Primers; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D016296:Mutagenesis; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D029721:Drosophila Proteins; C094492:DRL protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases