title: Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides.
abstract: The VIII Region of Bio-Bio is a major fruit-growing area of Chile that makes intensive use of agricultural pesticides. The cytogenetic damage associated with exposure to mixtures of pesticides was evaluated by comparing peripheral blood lymphocyte micronucleus (MN) frequencies in a group of 64 female agricultural workers and 30 female controls. The exposed subjects worked during the spring and summer in thinning and pruning fruit trees and in harvesting and packing different fruits, such as raspberries, grapes, apples, and kiwis. They did not use any protective measures during their work activities. A significant increase in the frequency of binucleated cells with micronuclei (BNMN) was found in the exposed women as compared with the controls (36.94 +/- 14.47 vs. 9.93 +/- 6.17 BNMN/1000 BN cells; P < 0.001). The frequency of BNMN varied as a function of age in both the exposed and control groups, but no correlation was found between BNMN frequency and the duration of exposure. Also, smoking and other habits had no effect on MN frequency. Our study confirms that occupational exposure to pesticide mixtures results in cytogenetic damage.
authors.affiliation: Laboratorio de Citogenética y Genética Toxicológica, Departamento de Biología Celular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.
authors.forename: Carolina
authors.initials: C
authors.lastname: Márquez
authors.forename: Cecilia
authors.initials: C
authors.lastname: Villalobos
authors.forename: Susana
authors.initials: S
authors.lastname: Poblete
authors.forename: Eva
authors.initials: E
authors.lastname: Villalobos
authors.forename: María
authors.initials: M
authors.lastname: de Los Angeles García
authors.forename: Soledad
authors.initials: S
authors.lastname: Duk
mesh_terms: D000367:Age Factors; D016573:Agrochemicals / Q000633:toxicity; D016022:Case-Control Studies; D002677:Chile; D002869:Chromosome Aberrations / Q000139:chemically induced*; D005260:Female; D006801:Humans; D015162:Micronucleus Tests; D016273:Occupational Exposure; D010575:Pesticides / Q000633:toxicity*; D013997:Time Factors
chemical_list: D016573:Agrochemicals; D010575:Pesticides