Polymer cushions functionalized with lipid molecules.
This Letter reports a novel approach to the fabrication of a biomimicking surface by modification of an end-functionalizable smooth polymer cushion constructed via chemoselective ligation with a phospholipid-like molecule containing oxyamine groups. The mobility of a phospholipid bilayer formed by vesicle fusion on the phospholipid-like molecule terminated polymer film was characterized by fluorescence recovery after bleaching. Platelet adhesion, as one measure of biocompatibility of the film was also studied and compared to other surfaces such as polyethylene or poly(dimethylsiloxane). The results show that the end-functionalized smooth polymer cushion has potential as a biocompatible platform to reconstitute membrane proteins.
Lee
Dong-Chan
DC
Department of Chemistry and The James Frank Institute, The University of Chicago, 5735 South Ellis Avenue, Chicago, Illinois 60637, USA.
Chang
Bong-Jun
BJ
Yu
Luping
L
Frey
Shelli L
SL
Lee
Ka Yee C
KY
Patchipulusu
Sirisha
S
Hall
Connie
C
D036681:Fluorescence Recovery After Photobleaching; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D010973:Platelet Adhesiveness; D011108:Polymers / Q000737:chemistry*
D008051:Lipid Bilayers; D008055:Lipids; D011108:Polymers