title: Detection of aptamer-protein interactions using QCM and electrochemical indicator methods.
abstract: We report novel method of detection thrombin-aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity.
authors.affiliation: Faculty of Mathematics, Physics and Computer Sciences, Comenius University, 842 48 Bratislava, Slovakia. hianik@fmph.uniba.sk
authors.forename: Tibor
authors.initials: T
authors.lastname: Hianik
authors.forename: Veronika
authors.initials: V
authors.lastname: Ostatná
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Zajacová
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Stoikova
authors.forename: Gennady
authors.initials: G
authors.lastname: Evtugyn
mesh_terms: D052157:Aptamers, Nucleotide; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D008751:Methylene Blue / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D011791:Quartz; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; D011506:Proteins; C081676:thrombin aptamer; D011791:Quartz; D004247:DNA; D013917:Thrombin; D008751:Methylene Blue