Effects of green tea catechin-induced lipolysis on cytosol glycerol content in differentiated 3T3-L1 cells.
The influence of catechins in green tea on lipolysis in fully differentiated 3T3-L1 cells was studied and glycerol release into the buffer, cytosol and residual triglyceride were measured. The addition of (-)-epigallocatechin-3-gallate (EGCG) stimulated glycerol release into the cytosol significantly after incubation for 4 h, but had no effect on that into the buffer. However, (+)-catechins did not produce a significant increase in lipolysis. These data suggested that EGCG has strong lipolytic activity.
Mochizuki
Miyako
M
Department of Health and Nutrition, School of Health and Human Life, Nagoya Bunri University, Inazawa, Japan.
Hasegawa
Noboru
N
D041721:3T3-L1 Cells / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002392:Catechin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D003600:Cytosol / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005990:Glycerol / Q000378:metabolism*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D013662:Tea
D000960:Hypolipidemic Agents; D010936:Plant Extracts; D013662:Tea; D002392:Catechin; D005990:Glycerol