title: [Determination of four alkaloids in Berberis plants by HPLC].
abstract: OBJECTIVE
  In order to assess Berberis, a simple and effective high-performance liquid chromatographic (HPLC) method was established.
  
  
  METHODS
  By applying HPLC with gradiation elution, this method was developed for determination of four kinds of alkaloids including berbamine, Jatrorrhizine, berberine and palmatine in methanolic extracts from root bark, root, stem bark and stem of Berberis.
  
  
  RESULTS
  The alkaloids berbamine, jatrorrhizine, berberine and palmatine shown good linear correlations in the range of 0.028-4.74 microg (r = 0.9998); 0.012-2.0 microg (r = 0.9996); 0.026-0.52 microg (r = 0.9999); 0.015-2.56 microg (r = 0.9998), respectively. The correlation coefficients of the calibration curve for the analytes exceeded 0.9998.
  
  
  CONCLUSIONS
  The optimized HPLC method was applied to analyze various samples, and the results shown that the content of alkaloids were obvious difference in Berberis from different area, different species and different parts.
authors.affiliation: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China. ddlliu@yahoo.com.cn
authors.forename: Duo-long
authors.initials: DL
authors.lastname: Di
authors.forename: Ye-wei
authors.initials: YW
authors.lastname: Liu
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Ma
authors.forename: Sheng-xiang
authors.initials: SX
authors.lastname: Jiang
mesh_terms: D000470:Alkaloids / Q000032:analysis; D044182:Benzylisoquinolines / Q000032:analysis; D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis*; D028142:Berberis / Q000737:chemistry* / Q000145:classification; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D013045:Species Specificity
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D001600:Berberine Alkaloids; C055785:jatrorrhizine; D001599:Berberine; C005413:palmatine; C027870:berbamine