Antimutagenic effects of extracts from sage (Salvia officinalis) in mammalian system in vivo.
Naturally occurring antimutagenic factors, especially those of plant origin, have recently become a subject of intensive research. Antimutagenic properties of terpenoid fractions of sage (Salvia officinalis) were tested in mammalian system in vivo through examining the ability of sage to decrease the frequency of aberrant cells induced by a potent mutagen. First, groups of mice were treated with three concentrations of sage alone and it was established that the frequency of aberrant cells after treatment with a concentration of 25 microL/kg was not significantly different from the negative control (olive oil), while that found after treatment with the 50 microL/kg concentration differed significantly (chi2(1) = 4.05, p < 0.05). Sage used at a concentration of 100 microL/kg was cytotoxic. Mitomycin C (MMC), known as a potent mutagen, was used for induction of chromosome aberrations. Post-treatment with sage suppressed the effects of MMC significantly. Both concentrations (25 microL/kg and 50 microL/kg) produced a significant decrease in the frequency of aberrations relative to MMC (chi2(1) = 5.42, p < 0.02, chi2(1) = 14.93, p < 0.001, respectively). The percent of aberrations decreased with increasing concentrations of sage. Only nontoxic concentrations of sage without mutagenic effects can be recommended for use as inhibitors of mutagenesis or carcinogenesis.
Vujosević
M
M
Department of Genetics, Institute for Biological Research Sinisa Stanković, 29 novembra 142, 11060 Belgrade, Serbia and Montenegro. mladenvu@ibiss.bg.ac.yu
Blagojević
Jelena
J
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D016685:Mitomycin / Q000037:antagonists & inhibitors; D016296:Mutagenesis / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D027543:Salvia officinalis
D016587:Antimutagenic Agents; D010936:Plant Extracts; D016685:Mitomycin