Measurement of serum pralidoxime methylsulfate (Contrathion) by high-performance liquid chromatography with electrochemical detection.
Pralidoxime methylsulfate (Contrathion) is widely used to treat organophosphate poisoning. Despite animal and human studies, the usefulness of Contrathion therapy remains a matter of debate. Therapeutic dosage regimens need to be clarified and availability of a reliable method for plasma pralidoxime quantification would be helpful in this process. We here describe a high-performance liquid chromatography technique with electrochemical detection to measure pralidoxime concentrations in human serum using guanosine as an internal standard. The assay was linear between 0.25 and 50 microg mL(-1) with a quantification limit of 0.2 microg mL(-1). The analytical precision was satisfactory, with variation coefficients lower 10%. This assay was applied to the analysis of a serum from an organophosphorate poisoned patient and treated by Contrathion infusions (100 and 200 mg h(-1)) after a loading dose (400 mg).
Houzé
Pascal
P
Laboratoire de Biochmie A, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010 Paris, France. pascal.houze@sls.ap-hop-paris.fr
Borron
Stephen W
SW
Scherninski
François
F
Bousquet
Bernard
B
Gourmel
Bernard
B
Baud
Frédéric
F
D002021:Buffers; D002801:Cholinesterase Reactivators / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D011220:Pralidoxime Compounds / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002021:Buffers; D002801:Cholinesterase Reactivators; D011220:Pralidoxime Compounds; C028797:pralidoxime