Polysorbate 80 increases the susceptibility to oxidative stress in rat thymocytes.
Effect of simultaneous application of polysorbate 80, a nonionic surfactant widely used in pharmaceutical products, and hydrogen peroxide on rat thymocytes was examined to see if polysorbate 80 increases the susceptibility to oxidative stress because this surfactant decreases the cellular content of glutathione. Polysorbate 80 at clinically-relevant concentrations increases the cytotoxicity of hydrogen peroxide under the in vitro condition. Result suggests that polysorbate 80 may increase the susceptibility of cells to oxidative stress.
Tatsuishi
Tomoko
T
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
Oyama
Yasuo
Y
Iwase
Kyoko
K
Yamaguchi
Jun-ya
JY
Kobayashi
Masako
M
Nishimura
Yumiko
Y
Kanada
Aimi
A
Hirama
Seigo
S
D000814:Aniline Compounds; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005079:Excipients / Q000633:toxicity*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D011136:Polysorbates / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology*; D014966:Xanthenes
D000814:Aniline Compounds; D017304:Annexin A5; D005079:Excipients; D005456:Fluorescent Dyes; D011136:Polysorbates; D014966:Xanthenes; C059715:Fluo-3; D006861:Hydrogen Peroxide