Regulation of receptor protein-tyrosine phosphatase dimerization.
Receptor protein-tyrosine phosphatases (RPTPs) are single membrane spanning proteins belonging to the family of PTPs that, together with the antagonistically acting protein-tyrosine kinases (PTKs), regulate the protein phosphotyrosine levels in cells. Protein-tyrosine phosphorylation is an important post-translational modification that has a major role in cell signaling by affecting protein-protein interactions and enzymatic activities. Increasing evidence indicates that RPTPs, like RPTKs, are regulated by dimerization. For RPTPalpha, we have shown that rotational coupling of the constitutive dimers in the cell membrane determines enzyme activity. Furthermore, oxidative stress, identified as an important second messenger during the past decade, is a regulator of rotational coupling of RPTPalpha dimers. In this review, we discuss the biochemical and cell biological techniques that we use to study the regulation of RPTPs by dimerization. These techniques include (co-) immunoprecipitation, RPTP activity assays, chemical and genetic cross-linking, detection of cell surface proteins by biotinylation, and analysis of RPTPalpha dimers, using conformation-sensitive antibody binding.
van der Wijk
Thea
T
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Blanchetot
Christophe
C
den Hertog
Jeroen
J
D000818:Animals; D001710:Biotin / Q000378:metabolism; D003432:Cross-Linking Reagents; D019281:Dimerization; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000032:analysis / Q000378:metabolism*
D003432:Cross-Linking Reagents; D001710:Biotin; D006861:Hydrogen Peroxide; D017027:Protein Tyrosine Phosphatases