Engineering an improved acellular nerve graft via optimized chemical processing.
The long-term goal of our research is to engineer an acellular nerve graft for clinical nerve repair and for use as a model system with which to study nerve-extracellular matrix interactions during nerve regeneration. To develop this model acellular nerve graft we (1) examined the effects of detergents on peripheral nerve tissue, and (2) used that knowledge to create a nerve graft devoid of cells with a well-preserved extracellular matrix. Using histochemistry and Western analysis, the impact of each detergent on cellular and extracellular tissue components was determined. An optimized protocol was created with the detergents Triton X-200, sulfobetaine-16, and sulfobetaine-10. This study represents the most comprehensive examination to date of the effects of detergents on peripheral nerve tissue morphology and protein composition. Also presented is an improved chemical decellularization protocol that preserves the internal structure of native nerve more than the predominant current protocol.
Hudson
Terry W
TW
Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712, USA.
Liu
Stephen Y
SY
Schmidt
Christine E
CE
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002474:Cell-Free System / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D019737:Transplants
D003902:Detergents