Odontoclasts in the Chinook salmon differ from mammalian odontoclasts by exhibiting a great proportion of cells with high nuclei number.
Odontoclasts resorbing teeth are multinucleated cells. Previously, the authors have investigated the distribution of number of nuclei per human odontoclast and showed that the mean number of nuclei per cell is 5.3, the median is 4, and 93.8% of cells have 10 or fewer nuclei. Teleost odontoclasts have features similar to those of mammals; however, the distribution of number of nuclei per cell remains unknown. The present study aimed to examine the distribution of number of nuclei per odontoclast in a teleost fish, Chinook salmon, Oncorhynchus tshawytscha (Walbaum), and to clarify the difference of number of nuclei in odontoclasts between Chinook salmon and humans. The maxillae and mandibles of Chinook salmon were fixed, decalcified, and embedded in Epon 812. Specimens were serially sectioned into 0.5-microm semithin sections and examined by light microscopy. Cells possessing a brush border adjacent to a resorptive lacuna were identified as odontoclasts, and 246 odontoclasts were investigated to determine the distribution of nuclei per cell. The mean number of nuclei per cell was 21.8 and the median was 17; only 24.4% of odontoclasts had 10 or fewer nuclei, and 95.5% had 50 or fewer nuclei. These results suggest that the range for the number of nuclei per odontoclast in Chinook salmon is greater than that in humans.
Domon
Takanori
T
Division of Developmental Biology of Hard Tissue, Department of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Kita 13, Nishi 7, 060-8586, Kita-Ku, Sapporo, Japan. tdomon@den.hokudai.ac.jp
Fukui
Ami
A
Taniguchi
Yumi
Y
Suzuki
Reiko
R
Takahashi
Shigeru
S
Yamamoto
Tsuneyuki
T
Wakita
Minoru
M
D000818:Animals; D001862:Bone Resorption; D002452:Cell Count; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure*; D015726:Giant Cells / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D046529:Microscopy, Electron, Transmission; D010010:Osteoclasts / Q000502:physiology / Q000648:ultrastructure*; D012474:Salmon / Q000033:anatomy & histology* / Q000502:physiology; D014070:Tooth / Q000502:physiology / Q000648:ultrastructure*