title: In vitro genotoxicity of exhaust emissions of diesel and gasoline engine vehicles operated on a unified driving cycle.
abstract: Acetone extracts of engine exhaust particulate matter (PM) and of vapor-phase semi-volatile organic compounds (SVOCs) collected from a set of 1998-2000 model year normal emitter diesel engine automobile or light trucks and from a set of 1982-1996 normal emitter gasoline engine automobiles or light trucks operated on the California Unified Driving Cycle at 22 [degree]C were assayed for in vitro genotoxic activities. Gasoline and diesel PM were comparably positive mutagens for Salmonella typhimurium strains YG1024 and YG1029 on a mass of PM extract basis with diesel higher on a mileage basis; gasoline SVOC was more active than diesel on an extracted-mass basis, with diesel SVOC more active on a mileage basis. For chromosomal damage indicated by micronucleus induction in Chinese hamster lung fibroblasts (V79 cells), diesel PM expressed about one-tenth that of gasoline PM on a mass of extract basis, but was comparably active on a mileage basis; diesel SVOC was inactive. For DNA damage in V79 cells indicated by the single cell gel electrophoresis (SCGE) assay, gasoline PM was positive while diesel PM was active at the higher doses; gasoline SVOC was active with toxicity preventing measurement at high doses, while diesel SVOC was inactive at all but the highest dose.
authors.affiliation: National Institute for Occupational Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505, USA.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Liu
authors.forename: Michael
authors.initials: M
authors.lastname: Keane
authors.forename: Mang
authors.initials: M
authors.lastname: Ensell
authors.forename: William
authors.initials: W
authors.lastname: Miller
authors.forename: Michael
authors.initials: M
authors.lastname: Kashon
authors.forename: Tong-man
authors.initials: TM
authors.lastname: Ong
authors.forename: Joe
authors.initials: J
authors.lastname: Mauderly
authors.forename: Doug
authors.initials: D
authors.lastname: Lawson
authors.forename: Mridul
authors.initials: M
authors.lastname: Gautam
authors.forename: Barbara
authors.initials: B
authors.lastname: Zielinska
authors.forename: Kevin
authors.initials: K
authors.lastname: Whitney
authors.forename: James
authors.initials: J
authors.lastname: Eberhardt
authors.forename: William
authors.initials: W
authors.lastname: Wallace
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D002140:California; D019457:Chromosome Breakage; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005742:Gasoline / Q000633:toxicity*; D008168:Lung / Q000378:metabolism; D009152:Mutagenicity Tests; D009930:Organic Chemicals / Q000378:metabolism / Q000633:toxicity; D001335:Vehicle Emissions / Q000633:toxicity*; D014835:Volatilization
chemical_list: D000393:Air Pollutants; D005742:Gasoline; D009930:Organic Chemicals; D001335:Vehicle Emissions