[Effects of XW630 on the expression of type I collagen protein in the epiphyseal plates of mice].
OBJECTIVE
To identify the effect of XW630 on the expression of type I collagen in the epiphyseal plates of mice.


METHODS
The immunohistochemical method was used to measure the change of type I collagen protein after being treated with XW630.


RESULTS
With 10(-7) mol/L or 10(-7) mol/L of XW630, the area of positive stain cells in every zone increased significantly compared to the control group. A more significant increase of the area of positive cells in the proliferative zone and hypertrophic zone was observed in the XW630 group than in the estrone group at a concentration of 10(-7) mol/L. When the XW630 was decreased to 10(-9) mol/L, the increase of positive cells was only found in the hypertrophic zone. The area of positive cells increased with the concentration of XW630.


CONCLUSIONS
The XW630 upregulates the dose-dependent expression of type I collagen protein in the resting zone, proliferative zone, and hypertrophic zone in the epiphyseal plates of mice in vitro.
Li
Lingzhi
L
Department of Medicinal Chemistry, West China Pharmaceutical College, Sichuan University, Chengdu 610041, China.
Weng
Lingling
L
Zhang
Yongliang
Y
Lou
Rongliang
R
Zheng
Hu
H
D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D006132:Growth Plate / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D009924:Organ Culture Techniques; D010879:Piperazines / Q000494:pharmacology*; D013754:Tetracyclines / Q000494:pharmacology*; D014457:Ulna / Q000378:metabolism
C428143:2-(3-estrone-N-ethyl-piperazine-methyl)tetracycline; D024042:Collagen Type I; D010879:Piperazines; D013754:Tetracyclines