[Preliminary study on cellular status of selenium and oxidative stress in human hepatocellular carcinoma and normal liver].
To investigate the roles of essential trace element selenium and cellular antioxidative systems in human hepatocellular carcinoma, we analyzed cellular distribution of selenium and assayed cytosolic and mitochondrial superoxide dismutase, glutathione peroxidase, thioredoxin reductase, glutathione and total protein thiols in 10 control healthy subjects, 6 cases of hepatocellular carcinoma and 2 cases of normal liver adjacent to the hepatocellular carcinoma. In hepatoma tissues, the Se contents in lysosome (P < 0.05), microsome (P < 0.05) and cytosol were higher than in the control liver. In 2 cases, normal liver adjacent to hepatocellular carcinoma had decreased Se content. In hepatoma tissues, GSH and protein thiols content and activities of SOD, GSH-Px and TrxR were all much higher than in normal liver tissue. These findings suggested the antioxidative defense-related enzymes and antioxidant were largely regulated in hepatoma cells, whereas the cause is not clear and requires further studies.
Chen
Chunying
C
Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100039, China.
Deng
Guilong
G
Zhao
Jiujiang
J
Gao
Yuxi
Y
Zhang
Peiqun
P
Li
Bai
B
Chai
Zhifang
Z
Peng
Shuyou
S
D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000503:physiopathology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000473:pathology / Q000503:physiopathology*; D018384:Oxidative Stress / Q000502:physiology*; D012643:Selenium / Q000378:metabolism / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism
D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D013880:Thioredoxin-Disulfide Reductase; D005978:Glutathione; D012643:Selenium