title: Burkholderia phenoliruptrix sp. nov., to accommodate the 2,4,5-trichlorophenoxyacetic acid and halophenol-degrading strain AC1100.
abstract: Strain AC1100 is well-known for its ability to degrade a variety of recalcitrant xenobiotics, including 2,4,5-trichlorophenoxyacetic acid. We performed a polyphasic-taxonomic study to determine its taxonomic position. The G+C content of strain AC1100 was 62.6 mol%. On the basis of 16S rRNA gene sequence similarity, strain AC1100 belonged to the beta-Proteobacteria and was most closely related to Burkholderia fungorum (98.3% similarity). DNA-DNA hybridisations, comparison of protein profiles, cellular fatty acid analysis and biochemical tests allowed genotypic and phenotypic differentiation of strain AC1100 from other Burkholderia species. Our data show that strain AC1100 represents a novel species for which the name Burkholderia phenoliruptrix sp. nov. is proposed. The type strain is AC1100T (= LMG 22037T = CCUG 48558T).
authors.affiliation: Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium. Tom.Coenye@UGent.be
authors.forename: Tom
authors.initials: T
authors.lastname: Coenye
authors.forename: Deborah
authors.initials: D
authors.lastname: Henry
authors.forename: David P
authors.initials: DP
authors.lastname: Speert
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000145:classification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010636:Phenols / Q000378:metabolism*; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010636:Phenols; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D015262:Xenobiotics; D015085:2,4,5-Trichlorophenoxyacetic Acid