Extended virulence genotypes and phylogenetic background of Escherichia coli isolates from patients with cystitis, pyelonephritis, or prostatitis.
Molecular analysis of 63 Escherichia coli urine isolates showed that pyelonephritis (n=23) and prostatitis (n=17) isolates exhibited more virulence factors (VFs) among the 35 sought than did cystitis isolates (n=23). Several nontraditional VFs--including bmaE (M fimbriae), gafD (G fimbriae), fyuA (yersiniabactin receptor), ireA and iroN (novel siderophore receptors), cvaC (colicin [microcin] V), traT (serum-resistance associated), ibeA (invasion of brain endothelium), ompT (outer membrane protease T), and malX (pathogenicity island marker)--either differentiated significantly between syndromes (despite small numbers of isolates and possible multiple-comparison artifacts) or were broadly prevalent. Thus, interventions that target conserved uro-VFs may be possible, despite the likely existence of syndrome-specific pathogenetic mechanisms and/or host defense systems.
Johnson
James R
JR
Medical Service, VA Medical Center, Minneapolis, MN 55417, USA. johns007@umn.edu
Kuskowski
Michael A
MA
Gajewski
Abby
A
Soto
Sara
S
Horcajada
Juan Pablo
JP
Jimenez de Anta
M Teresa
MT
Vila
Jordi
J
D018829:Adhesins, Bacterial / Q000235:genetics; D000328:Adult; D000368:Aged; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001430:Bacteriocins / Q000235:genetics; D003556:Cystitis / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics; D044404:Genomic Islands / Q000235:genetics; D006801:Humans; D037102:Lectins / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008875:Middle Aged; D010447:Peptide Hydrolases; D010636:Phenols / Q000032:analysis; D018272:Porins / Q000235:genetics; D011472:Prostatitis / Q000382:microbiology*; D011704:Pyelonephritis / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000235:genetics; D013030:Spain; D013844:Thiazoles / Q000032:analysis; D014556:Urine / Q000382:microbiology; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics*
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001430:Bacteriocins; C096350:CusC protein, E coli; D029968:Escherichia coli Proteins; C092493:GafD protein, E coli; C437328:IreA protein, E coli; D037102:Lectins; D008565:Membrane Proteins; D010636:Phenols; D018272:Porins; D011956:Receptors, Cell Surface; D013844:Thiazoles; C036297:TraT protein, E coli; D037521:Virulence Factors; C499588:ompT protein, E coli; C035902:siderophore receptors; C104398:yersiniabactin; C020411:microcin; D036781:Fimbriae Proteins; D010447:Peptide Hydrolases