Effect of dual ion implantation of calcium and phosphorus on the properties of titanium.
This study is concerned with the effect of dual implantation of calcium and phosphorus upon the structure, corrosion resistance and biocompatibility of titanium. The ions were implanted in sequence, first Ca and then P, both at a dose of 10(17) ions/cm2 at a beam energy of 25 keV. Transmission electron microscopy was used to investigate the microstructure of the implanted layer. The chemical composition of the implanted layer was examined by XPS and SIMS. The corrosion resistance was determined by electrochemical methods in a simulated body fluid (SBF) at a temperature of 37 degrees C. The biocompatibility tests were performed in vitro in a culture of human-derived bone cells (HDBC) in contact with the tested materials. The viability of the cells was determined by an XTT assay and their activity by the measurements of the alkaline phosphatase activity in contact with implanted and non-implanted titanium samples. The in vitro examinations confirmed that, under the conditions prevailing during the experiments, the biocompatibility of Ca + P ion-implanted titanium was satisfactory. TEM results show that the surface layer formed by the Ca + P implantation is amorphous. The corrosion resistance of titanium, examined by the electrochemical methods, appeared to be increased after the Ca + P ion implantation.
Krupa
D
D
Department of Materials Science and Engineering, Warsaw University of Technology. Wołoska 141, 02-507 Warsaw, Poland. dkrup@meil.pw.edu.pl
Baszkiewicz
J
J
Kozubowski
J A
JA
Barcz
A
A
Sobczak
J W
JW
Biliński
A
A
Lewandowska-Szumieł
M
M
Rajchel
B
B
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D002118:Calcium / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry; D002470:Cell Survival; D002478:Cells, Cultured; D003343:Corrosion; D004563:Electrochemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000494:pharmacology; D007477:Ions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010758:Phosphorus / Q000737:chemistry*; D013696:Temperature; D013778:Tetrazolium Salts / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D007202:Indicators and Reagents; D007477:Ions; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D010758:Phosphorus; C020243:calcium phosphate; D014025:Titanium; D000469:Alkaline Phosphatase; D002118:Calcium