title: Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata.
abstract: Nitrate at 70 mM gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3- molar ratio of 5:1 (giving a total of 40 mM N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l(-1), 0.36 mg g(-1), and 12.8 mg l(-1), respectively.
authors.affiliation: Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, P.R. China.
authors.forename: Xue-Wu
authors.initials: XW
authors.lastname: Pan
authors.forename: Heng-Hao
authors.initials: HH
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Ying-Tang
authors.initials: YT
authors.lastname: Lu
mesh_terms: D029769:Camptotheca / Q000254:growth & development* / Q000378:metabolism*; D002166:Camptothecin / Q000096:biosynthesis* / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation
chemical_list: D002166:Camptothecin