title: Neurokinin-1 receptor interacts with PrP(106-126)-induced dendritic cell migration and maturation.
abstract: Circulating monocytic cells may mediate neuroinvasion in transmissible spongiform encephalopathies by transporting prion protein (PrP) from sites of entry to the nervous system. Factors regulating monocyte-derived dendritic cell (DC) functions in neuronal tissue include neurogenic mediators, but their interactions with prion-infected DCs are unknown. Here, we report that neuropeptides regulate the interaction of DCs exposed to PrP(106-126). Inhibition of neurokinin-1-receptors activation with neurokinin-1-receptor antagonist or antibody attenuates substance P-induced enhancement of DC migration induced by PrP(106-126) and prion-protein-induced DC maturation. Other neuropeptides arrest chemotaxis. Data support a priming role of the neuropeptide substance P in PrP(106-126)-induced migration of DCs involving neurokinin-1-receptors.
authors.affiliation: Division of General Internal Medicine, Department of Internal Medicine, Medical University Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Kaneider
authors.forename: Arthur
authors.initials: A
authors.lastname: Kaser
authors.forename: Stefan
authors.initials: S
authors.lastname: Dunzendorfer
authors.forename: Herbert
authors.initials: H
authors.lastname: Tilg
authors.forename: Josef R
authors.initials: JR
authors.lastname: Patsch
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Wiedermann
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D018122:B7-1 Antigen / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D003713:Dendritic Cells / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005434:Flow Cytometry / Q000379:methods; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D064729:Neurokinin-1 Receptor Antagonists; D009479:Neuropeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D011328:Prions / Q000494:pharmacology*; D018040:Receptors, Neurokinin-1 / Q000276:immunology / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D000906:Antibodies; D018122:B7-1 Antigen; D006684:HLA-DR Antigens; D064729:Neurokinin-1 Receptor Antagonists; D009479:Neuropeptides; D010446:Peptide Fragments; D011328:Prions; D018040:Receptors, Neurokinin-1; C082632:prion protein (106-126)