Acridones and quinacridones: novel fluorophores for fluorescence lifetime studies.
Two new families of fluorescent probe, acridones and quinacridones, whose fluorescence lifetime can be altered to produce a range of lifetimes from 3 ns to 25 ns are described. Both families of fluorophore have fluorescence lifetimes which are unaffected by pH in the range of 5 to 9 and show a marked resistance to photobleaching. The probes have been modified to allow them to be attached to biomolecules and the labelling of a neuropeptide (substance P) is described. The labelled peptides have the same fluorescence lifetime as the free fluorophore. Quinacridone, with an emission around 550 nm offers a long fluorescence lifetime, photostable alternative to fluorescein.
Smith
J Anthony
JA
Amersham Biosciences, Amersham plc, The Maynard Centre, Forest Farm, Whitchurch, Cardiff CF14 7YT, United Kingdom. j.tony.smith@amersham.com
West
Richard M
RM
Allen
Malcolm
M
D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry*; D054831:Acridones; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013373:Substance P / Q000737:chemistry
D000166:Acridines; D054831:Acridones; D005456:Fluorescent Dyes; D006576:Heterocyclic Compounds, 4 or More Rings; C484590:quinacridone; D013373:Substance P; C041300:acridone