title: The Drosophila melanogaster condensin subunit Cap-G interacts with the centromere-specific histone H3 variant CID.
abstract: The centromere-specific histone H3 variant CENP-A plays a crucial role in kinetochore specification and assembly. We chose a genetic approach to identify interactors of the Drosophila CENP-A homolog CID. Overexpression of cid in the proliferating eye imaginal disk results in a rough eye phenotype, which is dependent on the ability of the overexpressed protein to localize to the kinetochore. A screen for modifiers of the rough eye phenotype identified mutations in the Drosophila condensin subunit gene Cap-G as interactors. Yeast two-hybrid experiments also reveal an interaction between CID and Cap-G. While chromosome condensation in Cap-G mutant embryos appears largely unaffected, massive defects in sister chromatid segregation occur during mitosis. Taken together, our results suggest a link between the chromatin condensation machinery and kinetochore structure.
authors.affiliation: Lehrstuhl für Genetik, University of Bayreuth, 95440 Bayreuth, Germany.
authors.forename: Hubert
authors.initials: H
authors.lastname: Jäger
authors.forename: Melanie
authors.initials: M
authors.lastname: Rauch
authors.forename: Stefan
authors.initials: S
authors.lastname: Heidmann
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002503:Centromere / Q000737:chemistry / Q000378:metabolism*; D000076247:Centromere Protein A; D020090:Chromosome Segregation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D018386:Kinetochores / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D008938:Mitosis; D046912:Multiprotein Complexes; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D021122:Protein Subunits; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012854:Sister Chromatid Exchange; D020798:Two-Hybrid System Techniques
chemical_list: D000076247:Centromere Protein A; C427237:Cid protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; D008840:Microfilament Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; C108312:condensin complexes; D000251:Adenosine Triphosphatases