title: Regulation of CRF, POMC and MC4R gene expression after electrical foot shock stress in the rat amygdala and hypothalamus.
abstract: We investigated the effects of electrical foot shock stress on the melanocortin signaling cascade and the hypothalamus-pituitary-adrenal (HPA) system by observing levels of mRNA expression of corticotropin releasing factor (CRF), pro-opiomelanocortin (POMC), and melanocortin receptor subtype 4 (MC4R) in the rat amygdala and hypothalamus. When rats were exposed to electrical shock for 0.5 hr or 1 hr, plasma ACTH and corticosterone concentrations increased, indicating stress. The rats were then sacrificed to obtain RNA preparations from the brain tissue. In the amygdala, the expression of MC4R and POMC mRNA as well as CRF mRNA was significantly increased by electrical foot shock stress. In the hypothalamus, MC4R and POMC mRNA increased, but CRF mRNA remained unchanged. The duration of increased gene expression of MC4R and POMC in the amygdala was more sustained than in the hypothalamus. These results have provided the first evidence that exposure to stress increases expression of the MC4R system in the amygdala and hypothalamus.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamano
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Toda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oshida
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Chaki
authors.forename: Kaori
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Isao
authors.initials: I
authors.lastname: Morishima
mesh_terms: D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D044105:Receptor, Melanocortin, Type 4 / Q000096:biosynthesis*; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D044105:Receptor, Melanocortin, Type 4; D011333:Pro-Opiomelanocortin; D003346:Corticotropin-Releasing Hormone