Effects of transforming growth interacting factor on biological behaviors of gastric carcinoma cells.
OBJECTIVE
Transforming growth interacting factor (TGIF) is an inhibitor of both transforming growth factor beta (TGF-beta) and retinoid signaling pathways. Moreover, the activation of MAPK pathway can prolong its half-life. However, its role in carcinogenesis is still unknown. Thus we attempted to investigate the effect of TGIF on biologic behaviors of gastric carcinoma cells.


METHODS
Gastric carcinoma cell line, SGC-7901, was stably transfected with plasmid PcDNA3.1-TGIF. Western blotting and cell immunohistochemistry screening for the highly expressing clone of TGIF were employed. The growth of transfected cells was investigated by MTT and colony-formation assays, and apoptosis was measured by flow cytometry (FCM) and transmission electron microscopy. Tumorigenicity of the transfectant cells was also analyzed.


RESULTS
TGIF had no effect on the proliferation, cell cycle and apoptosis of SGC-7901 cells, but cellular organelles of cells transfected with TGIF were richer than those of vector control or parental cells. Its clones were smaller than the control ones in plate efficiency, and its tumor tissues also had no obvious necrosis compared with the vector control or parental cells. Moreover, TGIF could resist TGF-beta mediated growth inhibition.


CONCLUSIONS
TGIF may induce differentiation of stomach neoplastic cells. In addition, TGIF can counteract the growth inhibition induced by TGF-beta.
Hu
Zhong-Liang
ZL
Department of Pathology, Basic Medical Institute, Central South University, Changsha 410078, Hunan Province, China.
Wen
Ji-Fang
JF
Xiao
De-Sheng
DS
Zhen
Hui
H
Fu
Chun-Yan
CY
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012176:Retinoids / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013274:Stomach Neoplasms / Q000503:physiopathology*; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics*
D012176:Retinoids; D016212:Transforming Growth Factor beta