title: In vitro assays of cell-mediated immunity in calves with persistent Mycobacterium avium infections do not correlate with delayed skin hypersensitivity.
abstract: A long-term kinetic study of the immune response of four calves experimentally infected with a M. avium strain was made using the following tests: lymphocyte stimulation (LS), leukocyte migrationinhibition (LMI), delayed skin hypersensitivity (DHS), and a radioimmunoassay (RIA) procedure for antibodies to M. avium. The cell-mediated immune (CMI) responses measured by these tests showed different courses during the infection period. The LS test showed several periods of peak values and the LMI test two peaks of responses of relatively shorter duration. At the end of the experimental period the DHS responses had decreased to insignificant levels, whereas the lymphocytes from all the calves responded in the LS test. No correlation could be detected between CMI and antibody mediated responses, and only two of the infected calves showed clear cut antibody responses measured by RIA. The results probably reflect the measurement of different aspects of the CMI response in the different test systems. The cyclical nature of the CMI measured by the LS and LMI tests may indicate the influence of regulatory mechanisms by suppressing lymphocyte sub-populations.
authors.affiliation: The National Veterinary Institute, Uppsala, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Bergman
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology*; D002464:Cell Migration Inhibition; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000662:veterinary*; D007111:Immunity, Cellular / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D009162:Mycobacterium avium / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial