Resistance of zwitterionic telomers accumulated on metal surfaces against nonspecific adsorption of proteins.
Telomers of N,N-dimethyl-N-(3-sulfopropyl)-3'-methacryloylaminopropanaminium inner salt (SPB), 2-methacryloyloxyethyl phosphorylcholine (MPC), and N,N-dimethyl-N-(1-carboxymethyl)-2'-methacryloyloxylethanaminium inner salt (CMB) were prepared by UV irradiation in the presence of N,N,N',N'-tetraethylthiuram disulfide and a disulfide-group-carrying iniferter (a compound which pursues initiation, chain transfer, and termination), Cys-BDC, which had been prepared by coupling N,N-diethyldithiocarbamoylmethylbenzoic acid succinimidyl ester with cystamine dihydrochloride. The telomers formed a self-assembled monolayer (SAM) on a gold electrode and a monolayer of colloidal gold deposited on a glass plate, as confirmed by the increase in potential difference (DeltaE(p)) of the voltammogram for hydroquinone as a probe using cyclic voltammetry (CV) and the increase in absorbance using localized surface plasmon resonance (LSPR) absorption spectroscopy, respectively. Nonspecific adsorption of various proteins onto the surfaces of various telomer SAMs was examined from the decrease in peak current (DeltaI) using CV and the absorption increase at 550 nm using LSPR absorption spectroscopy. The zwitterionic telomer SAM in general did not adsorb proteins significantly, suggesting the usability of zwitterionic polymer SAMs and brushes to coat various materials used in biomedical fields. The correlation between the structure of water in the vicinity of zwitterionic telomers and the resistance of the zwitterionic telomer SAMs against the nonspecific adsorption of proteins was discussed.
Kitano
Hiromi
H
Department of Chemical and Biochemical Engineering, Toyama University, Toyama 930-8555, Japan. kitano@eng.toyama-u.ac.jp
Kawasaki
Akio
A
Kawasaki
Hideaki
H
Morokoshi
Shinta
S
D000042:Absorption; D000327:Adsorption; D001703:Biophysics / Q000379:methods*; D001704:Biopolymers; D003102:Colloids / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry; D008956:Models, Chemical; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D011108:Polymers; D011199:Potentiometry; D011506:Proteins / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D013053:Spectrophotometry; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013997:Time Factors
D001704:Biopolymers; D003102:Colloids; D004220:Disulfides; D008689:Methacrylates; C511754:N,N-dimethyl-N-(1-carboxymethyl)-2'-methacryloyloxylethanaminium inner salt; C511752:N,N-dimethyl-N-(3-sulfopropyl)-3'-methacryloylaminopropanaminium inner salt; D011108:Polymers; D011506:Proteins; D012492:Salts; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D006046:Gold