Piperitone from Mentha longifolia var. chorodictya Rech F. reduces the nitrofurantoin resistance of strains of enterobacteriaceae.
The diluted essential oil of Mentha longifolia (L.) var. chlorodictya Rech F. foliage enhanced the bactericidal activity of nitrofurantoin decreasing the minimum inhibitory concentration (MIC) of nitrofurantoin for nitrofurantoin-resistant strains of Enterobacteriaceae. Thin-layer chromatography (TLC) analysis of the essential oil detected a fraction (R(f) = 0.35, UV lambda(max) of 232.5), which was the most effective in enhancement of nitrofurantoin activity. Using gas liquid chromatography and known standards, the active fraction was identified as piperitone. 1 microl of the piperitone fraction decreased the MIC of nitrofurantoin 3-20 fold for the different strains of Enterobacteriaceae tested.
Shahverdi
A R
AR
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. shahverd@sina.tums.ac.ir
Rafii
F
F
Tavassoli
F
F
Bagheri
M
M
Attar
F
F
Ghahraman
A
A
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000892:Anti-Infective Agents, Urinary / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000081005:Cyclohexane Monoterpenes; D004347:Drug Interactions; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects*; D006801:Humans; D027541:Mentha; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D009582:Nitrofurantoin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D000892:Anti-Infective Agents, Urinary; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D010938:Plant Oils; C496656:piperitone; D009582:Nitrofurantoin