title: [Detection of anti-Toxoplasma gondii IgG, IgM and IgA immunoglobulins in the serum, cerebrospinal fluid and saliva of patients with acquired immunodeficiency syndrome and neurotoxoplasmosis].
abstract: We studied 55 patients with acquired immunodeficiency syndrome (AIDS) and neurotoxoplasmosis (group 1), 37 patients with AIDS and neurological involvement due to another etiology (group 2) and 18 anti-HIV-negative individuals with neurological manifestations, by searching for anti-T. gondii IgG, IgA and IgM immunoglobulins in serum, cerebrospinal fluid (CSF)and saliva, using ELISA. The negative predictive value of the test for IgG in serum was 100% and in CSF, 92.4%. There was no difference among the three groups studied regarding IgA in serum. For IgA, in CSF the test reached 72.7% specificity (p<0.05). In saliva, only the detection of IgG was found to be correlated with a diagnosis of neurotoxoplasmosis. We emphasize that the absence of anti-T. gondii IgG antibodies in serum and CSF strongly indicates the absence of a diagnosis of neurotoxoplasmosis and that specific IgA immunoglobulins in CSF and IgG in saliva may represent two auxiliary markers for the differential diagnosis of toxoplasmic encephalitis in AIDS.
authors.affiliation: Centro de Ciências Biomédicas, Faculdade de Medicina, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. aerciosb@uol.com.br
authors.forename: Aercio Sebastião
authors.initials: AS
authors.lastname: Borges
authors.forename: José Fernando de Castro
authors.initials: JF
authors.lastname: Figueiredo
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000276:immunology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D007075:Immunoglobulin M / Q000097:blood / Q000134:cerebrospinal fluid; D008297:Male; D011237:Predictive Value of Tests; D012463:Saliva / Q000737:chemistry; D014122:Toxoplasma / Q000276:immunology*; D016781:Toxoplasmosis, Cerebral / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D015415:Biomarkers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M