title: Highly efficient gene expression of a fibrinolytic enzyme (subtilisin DFE) in Bacillus subtilis mediated by the promoter of alpha-amylase gene from Bacillus amyloliquefaciens.
abstract: Subtilisin DFE is a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4. The promoter and signal peptide-coding sequence of alpha-amylase gene from B. amyloliquefaciens was cloned and fused to the sequence coding for pro-peptide and mature peptide of subtilisin DFE. This hybrid gene was inserted into the Escherichia coli/Bacillus subtilis shuttle plasmid vector, pSUGV4. Recombinant subtilisin DFE gene was successfully expressed in B. subtilis WB600 with a fibrinolytic activity of 200 urokinase units ml(-1).
authors.affiliation: College of Life Sciences, Sichuan University, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xiao
authors.forename: Ren-Huai
authors.initials: RH
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.forename: Yi-Zheng
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020860:Subtilisin / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D000516:alpha-Amylases; D020860:Subtilisin