Visualization of immunosorption by scanning electron microscopy.
Immunosorption has become a very important biochemical and serological tool and it is the purpose of this paper to visualize this process qualitatively using the scanning electron microscope. Different carriers (i.e. CNBr activated cellulose and Sepharose, glutaraldehyde treated acrylamide-agarose beads Magnogel, and polystyrene cover slips) were coated with different antibodies and incubated with their homologous antigens such as pneumococci, ferritin, polymeric Salmonella flagellin and mechanically detached flagella, as well as tetanus toxoid, E. coli bacteriophage T4 and Rota virus particles. As negative controls the sorbents were incubated with heterologous antigens.
Stucki
M
M
Veterinary Bacteriological Institute, 3001 Berne, Switzerland.
Fey
H
H
D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000737:chemistry; D000941:Antigens / Q000032:analysis* / Q000737:chemistry; D007163:Immunosorbent Techniques; D008855:Microscopy, Electron, Scanning / Q000379:methods
D000906:Antibodies; D000941:Antigens