Induction of chromosomal aberrations and sister chromatid exchanges by chlormadinone acetate in human lymphocytes: a possible role of reactive oxygen species.
Genotoxicity study of a synthetic progestin chlormadinone acetate (CMA) was carried out in human lymphocytes using chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) as parameter. Effect of CMA was studied at 10, 20, 30 and 40 microM. CMA was genotoxic at 30 and 40 microM. With a view to study the possible mechanism of genotoxicity of CMA, superoxide dismutase (SOD) and catalase (CAT) were used separately and in combination along with the CMA (40 microM) at different doses. SOD treatment increased CAs and SCEs at both the doses. CAT treatment decreased the frequencies of CAs and SCEs in both, separately and in combination with SOD, suggesting a possible role of reactive oxygen species for the genotoxic damage.
Siddique
Yasir Hasan
YH
Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202 002, India. yasir_hasansiddique@rediffmail.com
Afzal
Mohammad
M
D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D002715:Chlormadinone Acetate / Q000009:adverse effects*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012854:Sister Chromatid Exchange; D013482:Superoxide Dismutase / Q000378:metabolism
D017382:Reactive Oxygen Species; D002715:Chlormadinone Acetate; D002374:Catalase; D013482:Superoxide Dismutase