[Study of the methods of determining the isoflavones in herbs of Trifolium pratense extractive].
OBJECTIVE
To study the methods of determining the amount of Isoflavones in red clover extractive.


METHODS
RP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.


RESULTS
After being water-solved, the four groups of flavoes elements satisfactorily separated, the amount of feeding in a range of 0.024-0.336 microg which has a good linear relationship with the peaks, and the total isoflavones determining results repetition, RSD=10.1%.


CONCLUSIONS
A simple, reliable and effective quality-control method is given.
Bian
Qing-quan
QQ
Mianyang Teacher's College, Mianyang 621000, Sichuan, China. Biam-55@sohu.com
Yang
Zhen-ping
ZP
Ma
Mei-zhong
MZ
Li
Song
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D029921:Trifolium / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C040641:genistin; C007768:formononetin; C013908:daidzin