Overexpression of the AP2/EREBP transcription factor OPBP1 enhances disease resistance and salt tolerance in tobacco.
Osmotin promoter binding protein 1 (OPBP1), an AP2/EREBP-like transcription factor of tobacco (Nicotiana tabacum), was isolated using a yeast one-hybrid system. RNA gel blot analysis indicated that expression of the OPBP1 gene was induced by elicitor cryptogein, NaCl, ethephon, methyl jasmonate, as well as cycloheximide. Transient expression analysis using an OPBP1-eGFP fusion gene in onion epidermal cells revealed that the OPBP1 protein was targeted to the nuclear. Further, electrophoretic mobility shift assays demonstrated that the recombinant OPBP1 protein could bind to an oligonucleotide containing the GCC-box cis element. Transgenic tobacco plants with an over expression of the OPBP1 gene accumulated high levels of PR-1a and PR-5d genes and exhibited enhanced resistance to infection by Pseudomonas syringae pv tabaci and Phytophthora parasitica var nicotianae pathogens. They also exhibited increased tolerance to salt stress. These results suggest that OPBP1 might be a transcriptional regulator capable of regulating expression in sets of stress-related genes.
Guo
Ze-Jian
ZJ
Biotechnology Institute, Zhejiang University, Hangzhou, PR China. guozj@cu.edu.cn
Chen
Xu-Jun
XJ
Wu
Xue-Long
XL
Ling
Jian-Qun
JQ
Xu
Ping
P
D000085:Acetates / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D020418:Algal Proteins / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D005656:Fungal Proteins; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics; D009154:Mutation; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology; D054883:Oxylipins; D010802:Phylogeny; D010838:Phytophthora / Q000254:growth & development; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D044224:Pseudomonas syringae / Q000254:growth & development; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D000085:Acetates; D020418:Algal Proteins; D003517:Cyclopentanes; D005656:Fungal Proteins; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C061032:cryptogein protein, Phytophthora cryptogea; D049452:Green Fluorescent Proteins; D012965:Sodium Chloride; C072239:methyl jasmonate; D003513:Cycloheximide; C005073:ethephon