title: Differences in LexA regulon structure among Proteobacteria through in vivo assisted comparative genomics.
abstract: The LexA regulon encompasses an ensemble of genes involved in preserving cell viability under massive DNA damage and is present in most bacterial phyla. Up to date, however, the scope of this network had only been assessed in the Gamma Proteobacteria. Here, we report the structure of the LexA regulon in the Alpha Proteobacteria, using a combined approach that makes use of in vitro and in vivo techniques to assist and validate the comparative genomics in silico methodology. This leads to the first experimentally validated description of the LexA regulon in the Alpha Proteobacteria, and comparison of regulon core structures in both classes suggests that a least common multiple set of genes (recA, ssb, uvrA and ruvCAB) might be a defining property of the Proteobacteria LexA network.
authors.affiliation: Biomedical Applications Group, Centro Nacional de Microelectrónica, 08193 Bellaterra, Spain.
authors.forename: Ivan
authors.initials: I
authors.lastname: Erill
authors.forename: Mónica
authors.initials: M
authors.lastname: Jara
authors.forename: Noelia
authors.initials: N
authors.lastname: Salvador
authors.forename: Marcos
authors.initials: M
authors.lastname: Escribano
authors.forename: Susana
authors.initials: S
authors.lastname: Campoy
authors.forename: Jordi
authors.initials: J
authors.lastname: Barbé
mesh_terms: D000465:Algorithms; D020561:Alphaproteobacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D019295:Computational Biology; D016384:Consensus Sequence; D020563:Gammaproteobacteria / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D012045:Regulatory Sequences, Nucleic Acid; D018085:Regulon; D013014:SOS Response, Genetics; D012697:Serine Endopeptidases / Q000378:metabolism*; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein