Two-faced cyclins with eyes on the targets.
We recently reported that the 'hydrophobic patch' (HP) of the Saccharomyces cerevisiae S-phase cyclin Clb5 facilitates its interaction with Orc6 (via its Cy or RXL motif), providing a mechanism that helps prevent rereplication from individual origins. This is the first finding of a biological function for an interaction between a cyclin and a cyclin-binding motif (Cy or RXL motif) in a target protein in Saccharomyces cerevisiae. It is also the first such example involving a B-type cyclin in any organism. Yet, some of our observations as well as work from other groups suggest that HP-RXL interactions are functionally important for cyclin-Cdk signaling to other targets. The evolutionary conservation of the HP motif suggests that it allows cyclins to carry out important and specialized functions.
Archambault
Vincent
V
Rockefeller University, New York, New York, USA.
Buchler
Nicolas E
NE
Wilmes
Gwendolyn M
GM
Jacobson
Matthew D
MD
Cross
Frederick R
FR
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D019926:Cyclin B / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000502:physiology; D016213:Cyclins / Q000235:genetics / Q000502:physiology; D004261:DNA Replication; D015966:Gene Expression Regulation, Fungal; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D051738:Origin Recognition Complex / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
C076558:CLB5 protein, S cerevisiae; D019926:Cyclin B; D016213:Cyclins; C497221:ORC6 protein, S cerevisiae; D051738:Origin Recognition Complex; D029701:Saccharomyces cerevisiae Proteins; D018844:Cyclin-Dependent Kinases