Proteomics analysis by two-dimensional differential gel electrophoresis reveals the lack of a broad response of Neisseria meningitidis to in vitro-produced AI-2.
To investigate the effect of the autoinducer AI-2 on protein expression in Neisseria meningitidis, a luxS mutant of strain MC58 was grown in the presence and absence of in vitro-produced AI-2, and differential protein expression was assessed by two-dimensional differential gel electrophoresis. N. meningitidis did not show a global response to AI-2 signaling activity.
Schauder
Stephan
S
Research Department, Microbiology, Aventis Pasteur, 1541 avenue Marcel Mérieux, 69280 Marcy l'Etoile, France.
Penna
Lucia
L
Ritton
Adeline
A
Manin
Catherine
C
Parker
Fabienne
F
Renauld-Mongénie
Geneviève
G
D001426:Bacterial Proteins / Q000032:analysis* / Q000502:physiology; D013437:Carbon-Sulfur Lyases; D015180:Electrophoresis, Gel, Two-Dimensional; D006714:Homoserine / Q000031:analogs & derivatives* / Q000502:physiology*; D007783:Lactones; D009345:Neisseria meningitidis / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria