Tuning the specificity of a synthetic receptor using a selected nucleic acid receptor.
Because of their relative simplicity, synthetic receptors often lack the selectivity observed for biopolymer receptors, such as aptamers. However, aptamer recognition of ligands is limited by the chemistries inherent in the four canonical nucleotides. Here, we report the design and selection of a ternary complex in which the specificity of a bis-boronic acid synthetic host (1) that binds to various carboxylic acids is tuned by a surrounding aptamer. Although, the synthetic receptor alone has higher selectivity for citrate over DL-tartrate, the formation of the aptamer:receptor complex reversed the organic host selectivity to preferentially bind tartrate. The RNA conformation changed upon the introduction of the synthetic host, consistent with an induced-fit mechanism for binding.
Manimala
Joseph C
JC
The University of Texas at Austin, Institute for Cellular and Molecular Biology. 1 University Station, A5300, University of Texas, Austin, Texas 78712, USA.
Wiskur
Sheryl L
SL
Ellington
Andrew D
AD
Anslyn
Eric V
EV
D001483:Base Sequence; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D006037:Glyoxal / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry*; D012685:Sepharose / Q000031:analogs & derivatives; D013379:Substrate Specificity; D013644:Tartrates / Q000737:chemistry
D001897:Boronic Acids; D009841:Oligonucleotides; D011956:Receptors, Cell Surface; D013644:Tartrates; D006037:Glyoxal; D012313:RNA; D012685:Sepharose; C029768:tartaric acid