title: [A quantitative analysis of complex formation between IgM and immobilized ligand using atomic force microscopy].
abstract: Specific interaction between human IgM and polyclonal antibodies immobilized on support was studied by atomic force microscopy. Human IgMs are responsible for a number of side effects arising during the xenotransplantation of mammalian organs to man. On the basis of atomic force microscopy, a quantitative analysis of complexes with IgM was performed. The data of the analysis agree well with the results of enzyme immunoassay. It was shown that the method of detection of immune complexes based on atomic force microscopy is able to detect specific antibodies/antigens in serum.
authors.forename: N V
authors.initials: NV
authors.lastname: Maliuchenko
authors.forename: I I
authors.initials: II
authors.lastname: Agapov
authors.forename: A G
authors.initials: AG
authors.lastname: Tonevitskiĭ
authors.forename: M M
authors.initials: MM
authors.lastname: Moĭsenovich
authors.forename: M N
authors.initials: MN
authors.lastname: Savvateev
authors.forename: E A
authors.initials: EA
authors.lastname: Gudim
authors.forename: V A
authors.initials: VA
authors.lastname: Bykov
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000097:blood; D002417:Cattle; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D008024:Ligands; D018625:Microscopy, Atomic Force; D011485:Protein Binding
chemical_list: D000538:Aluminum Silicates; D000906:Antibodies; D000936:Antigen-Antibody Complex; D007075:Immunoglobulin M; D008024:Ligands; C011934:mica