Beneficial effect of hyperbaric oxygen therapy on liver regeneration after 90% hepatectomy in rats.
OBJECTIVE
Hyperbaric oxygen therapy (HBOT) has been reported to augment oxygen delivery to ischemic tissues and improve the liver dysfunction in clinical cases. HBOT was performed after 90% hepatectomy in rats to determine its effect on the regeneration of remnant liver.


METHODS
After 90% hepatectomy was performed in 8-week-old male Wistar rats, the animals were subdivided into an HBOT (2 atm abs., 80% O2, 1 h/day, 3 days) group and a non-HBOT group. Members of both groups were sacrificed, usually every 4 h until a maximum of 50 h after hepatectomy, and the liver regeneration rate, the proportion of PCNA-positive cells and the ATP volume in the remnant tissues were examined.


RESULTS
In the HBOT group, the liver regeneration rate at 36 h and 50 h after operation and the proportion of PCNA positive cells at 8 h was significantly increased compared with the non-HBOT group. The ATP volume in the remnant livers in the HBOT group was also significantly increased at 12 h.


CONCLUSIONS
HBOT augmented liver regeneration after hepatectomy by stabilization of energy metabolism induced by oxygen delivery in rats.
Nagamine
K
K
Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan. kotarongmn@ytv.home.ne.jp
Kubota
T
T
Togo
S
S
Nagashima
Y
Y
Mori
M
M
Shimada
H
H
D000818:Animals; D004734:Energy Metabolism / Q000502:physiology; D006498:Hepatectomy; D006931:Hyperbaric Oxygenation; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D010100:Oxygen