Genomic dissection of the cell-type-specification circuit in Saccharomyces cerevisiae.
The budding yeast Saccharomyces cerevisiae has three cell types (a cells, alpha cells, and a/alpha cells), each of which is specified by a unique combination of transcriptional regulators. This transcriptional circuit has served as an important model for understanding basic features of the combinatorial control of transcription and the specification of cell type. Here, using genome-wide chromatin immunoprecipitation, transcriptional profiling, and phylogenetic comparisons, we describe the complete cell-type-specification circuit for S. cerevisiae. We believe this work represents a complete description of cell-type specification in a eukaryote.
Galgoczy
David J
DJ
Department of Biochemistry and Biophysics, University of California, 600 16th Street, San Francisco, CA 94143-2200, USA.
Cassidy-Stone
Ann
A
Llinás
Manuel
M
O'Rourke
Sean M
SM
Herskowitz
Ira
I
DeRisi
Joseph L
JL
Johnson
Alexander D
AD
D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D019143:Evolution, Molecular; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D009995:Osmosis; D010802:Phylogeny; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D014158:Transcription, Genetic
D012965:Sodium Chloride