Integrated microelectrode array and microfluidics for temperature clamp of sensory neurons in culture.
A device for cell culture is presented that combines MEMS technology and liquid-phase photolithography to create a microfluidic chip that influences and records electrical cellular activity. A photopolymer channel network is formed on top of a multichannel microelectrode array. Preliminary results indicated successful local thermal control within microfluidic channels and control of lamina position over the electrode array. To demonstrate the biological application of such a device, adult dissociated dorsal root ganglion neurons with a subpopulation of thermally-sensitive cells are attached onto the electrode array. Using laminar flow, dynamic control of local temperature of the neural cells was achieved while maintaining a constant chemical culture medium. Recording the expected altered cellular activity confirms the success of the integrated device.
Pearce
Thomas M
TM
Department of Biomedical Engineering, University of Wisconsin-Madison, WI, USA.
Wilson
J Adam
JA
Oakes
S George
SG
Chiu
Shing-Yan
SY
Williams
Justin C
JC
D000200:Action Potentials; D000818:Animals; D004867:Equipment Design; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009475:Neurons, Afferent / Q000166:cytology* / Q000502:physiology; D018408:Patch-Clamp Techniques; D013696:Temperature