Engineering protein A affinity chromatography.
Staphylococcal protein A can selectively interact with immunoglobulins. This protein is widely used as a ligand for affinity chromatography to purify therapeutic antibodies on an industrial scale. This type of affinity chromatography constitutes a generic step in processing antibodies. Questions of scale-up, design of chromatographic conditions, clearance of adventitious agents and operational modes, such as continuous operation or purification of antibodies in expanded-bed mode, will be addressed in this review.
Jungbauer
Alois
A
University of Natural Resources and Applied Life Sciences and Austrian Center of Biopharmaceutical Technology, Department of Biotechnology, Muthgasse 18, 1190 Vienna, Austria. alois.jungbauer@boku.ac.at
Hahn
Rainer
R
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D007074:Immunoglobulin G / Q000302:isolation & purification; D008024:Ligands; D008956:Models, Chemical; D015202:Protein Engineering; D013205:Staphylococcal Protein A / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D008024:Ligands; D013205:Staphylococcal Protein A