A role of MAP1B in Reelin-dependent neuronal migration.
The signaling cascades governing neuronal migration are believed to link extracellular signals to cytoskeletal components. MAP1B is a neuron-specific microtubule-associated protein implicated in the control of the dynamic stability of microtubules and in the cross-talk between microtubules and actin filaments. Here we show that Reelin can induce mode I MAP1B phosphorylation, both in vivo and in vitro, through gsk3 and cdk5 activation. Additionally, mDab1 participates in the signaling cascade responsible for mode I MAP1B phosphorylation. Conversely, MAP1B-deficient mice display an abnormal structuring of the nervous system, especially in brain laminated areas, indicating a failure in neuronal migration. Therefore, we propose that Reelin can induce post-translational modifications on MAP1B that could correlate with its function in neuronal migration.
González-Billault
Christian
C
Centro de Biología Molecular, Universidad Autonoma de Madrid-CSIC, Madrid 28049, Spain.
Del Río
José A
JA
Ureña
Jesús M
JM
Jiménez-Mateos
Eva M
EM
Barallobre
María J
MJ
Pascual
Marta
M
Pujadas
Lluís
L
Simó
Sergi
S
Torre
Anna La
AL
Gavin
Rosalina
R
Wandosell
Francisco
F
Soriano
Eduardo
E
Avila
Jesús
J
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D005260:Female; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C085284:microtubule-associated protein 1B; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases