Genome sequence of the non-pathogenic strain 15 of pneumonia virus of mice and comparison with the genome of the pathogenic strain J3666.
Pneumonia virus of mice (PVM) is a member of the subfamily Pneumovirinae and is the closest known relative of respiratory syncytial virus. Both viruses cause pneumonia in their respective hosts. Here, the genome sequences of two strains of PVM, non-pathogenic strain 15 and pathogenic strain J3666, are reported. Comparison of the genome sequences revealed 59 nucleotide differences between the two strains, 37 of which were coding. The nucleotide differences were spread throughout the genome, affecting cis-acting regulatory regions and seven of the ten genes. Development of a reverse-genetics system for PVM should allow further elucidation of the functional importance of the genetic differences between the two strains identified here.
Thorpe
L C
LC
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Easton
A J
AJ
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
D000595:Amino Acid Sequence; D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D029122:Murine pneumonia virus / Q000235:genetics*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins