Effect of 6 months' training on the reactive oxygen species production capacity of neutrophils and serum opsonic activity in judoists.
The effects of long-term training on the production of reactive oxygen species (ROS) from neutrophils and serum opsonic activity (SOA) remain to date unknown. The aim of this study was to evaluate the effect of 6 months training on ROS production and SOA in judoists. Fifty-six judoists were enrolled this study. White blood cell counts, serum creatine kinase (CK), asparate aminotransferase (ASAT), alanine aminotransferase (ALAT), lactate dehydrogenase (LDH) and ROS production from neutrophils, and serum opsonic activity (SOA) using the lucigenin and luminol probes, were measured before and after daily judo exercise (2 h) in March and September. The subjects started their training from March after no exercise for three months, and continued it for 6 months (until September). In March, myogenic enzymes such as CK, ASAT, LDH and neutrophil counts increased and immunoglobulins, complements and SOA decreased after daily judo exercise. Such significant changes were not seen in September. On the other hand, ROS significantly increased after daily judo exercise in both March and September, with no significant difference in the rates of change. In conclusion, 6 month training minimized the changes in SOA as well as muscle enzymes, neutrophil counts, serum immunoglobulins and complements. This could be categorized as a long-term training effect. However, no such change was seen in ROS.
Miura
Masashi
M
Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan.
Umeda
Takashi
T
Nakaji
Shigeyuki
S
Liu
Qiang
Q
Tanabe
Masaru
M
Kojima
Arata
A
Yamamoto
Yousuke
Y
Sugawara
Kazuo
K
D000293:Adolescent; D001823:Body Composition; D015444:Exercise; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology; D008297:Male; D016139:Martial Arts / Q000502:physiology*; D018482:Muscle, Skeletal / Q000201:enzymology; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D009895:Opsonin Proteins / Q000097:blood / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors
D007136:Immunoglobulins; D009895:Opsonin Proteins; D017382:Reactive Oxygen Species