title: The genetic basis of malathion resistance in housefly (Musca domestica L.) strains from Turkey.
abstract: Organophosphate insecticide (parathion/diazinon) resistance in housefly (Musca domestica L.) is associated with the change in carboxylesterase activity. The product of MdalphaE7 gene is probably playing a role in detoxification of xenebiotic esters. In our research, we have isolated, cloned and sequenced the MdalphaE7 gene from 5 different Turkish housefly strains. High doses of malathion (600 microg/fly) were applied in a laboratory environment for one year to Ceyhan1, Ceyhan2, Adana and Ankara strains while no insecticide treatment was performed in the laboratory to Kirazli strain. Trp251 --> Ser substitution was found in the product of MdalphaE7 gene in all malathion resistant and Kirazli stocks. In addition, we checked the malathion carboxylesterase (MCE), percent remaining activities in acetylcholinesterase (AChE), glutathion-S-transferase (GST), and general esterase activities in all 5 strains used in this study. In comparing with universal standard sensitive control WHO, a high level of MCE and GST activities were observed while lower level of general esterase activities was detected in the tested strains. In addition, a higher percent remaining activities in AChE than WHO susceptible strain were observed in all malathion resistant strains.
authors.affiliation: Department of Biology, Mugla University, Mugla, Turkey. vatantaskin@hotmail.com
authors.forename: V
authors.initials: V
authors.lastname: Taşkin
authors.forename: M
authors.initials: M
authors.lastname: Kence
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015139:Blotting, Southern; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006793:Houseflies / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics*; D008294:Malathion; D014421:Turkey
chemical_list: D005982:Glutathione Transferase; D002265:Carboxylic Ester Hydrolases; C021528:malathion esterases; D000110:Acetylcholinesterase; D008294:Malathion