Replication timing of human chromosome 6.
Genomic microarrays have been used to assess DNA replication timing in a variety of eukaryotic organisms. A replication timing map of the human genome has already been published at a 1Mb resolution. Here we describe how the same method can be used to assess the replication timing of chromosome 6 with a greater resolution using an array of overlapping tile path clones. We report the replication timing map of the whole of chromosome 6 in general, and the MHC region in particular. Positive correlations are observed between replication timing and a number of genomic features including GC content, repeat content and transcriptional activity.
Woodfine
Kathryn
K
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
Beare
David M
DM
Ichimura
Koichi
K
Debernardi
Silvana
S
Mungall
Andrew J
AJ
Fiegler
Heike
H
Collins
V Peter
VP
Carter
Nigel P
NP
Dunham
Ian
I
D002460:Cell Line; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics* / Q000502:physiology*; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000737:chemistry / Q000235:genetics; D042622:DNA Repeat Expansion; D042522:DNA Replication Timing; D044127:Epigenesis, Genetic; D016193:G1 Phase / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation; D006147:Guanine / Q000032:analysis; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016196:S Phase / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic
D006147:Guanine; D003596:Cytosine; D004247:DNA