Visual response properties of burst and tonic firing in the mouse dorsal lateral geniculate nucleus.
Thalamic relay cells fire action potentials in two modes: burst and tonic. Previous studies in cats have shown that these two modes are associated with significant differences in the visual information carried by spikes in the dorsal lateral geniculate nucleus (dLGN). Here we describe the visual response properties of burst and tonic firing in the mouse dLGN. Extracellular recordings of activity in single geniculate cells were performed under halothane and nitrous oxide anesthesia in vivo. After confirming that the criteria used to isolate burst spikes from these recordings identify firing events with properties described for burst firing in other species and preparations, we show that burst firing in the mouse dLGN occurs during visual stimulation. We then compare burst and tonic firing across a wide range of visual response characteristics. While the two firing modes do not differ with respect to spatial summation or spatial frequency tuning, they show significant differences in the temporal domain. Burst spikes are phase advanced relative to their tonic counterparts. Burst firing is also more rectified, possesses sharper temporal frequency tuning, and prefers lower temporal frequencies than tonic firing. In addition, contrast-response curves are more step-like for burst responses. Finally, we present analyses that describe the stimulus detection abilities and spike timing reliability of burst and tonic firing.
Grubb
Matthew S
MS
University Laboratory of Physiologyk, Parks Road, Oxford, OX1 3PT UK.
Thompson
Ian D
ID
D000200:Action Potentials / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D004558:Electric Stimulation; D005260:Female; D005583:Fourier Analysis; D005829:Geniculate Bodies / Q000166:cytology* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000145:classification / Q000502:physiology*; D010507:Periodicity; D010775:Photic Stimulation; D011336:Probability; D012372:ROC Curve; D011930:Reaction Time / Q000502:physiology; D012044:Regression Analysis; D015203:Reproducibility of Results; D013997:Time Factors