Xenopus tropicalis peroxidasin gene is expressed within the developing neural tube and pronephric kidney.
Peroxidasin, originally identified in Drosophila, is a member of the myeloperoxidase family with a novel domain structure. It is proposed that peroxidasin is secreted and has functions associated with stabilization of the extracellular matrix. We report the identification of the Xenopus tropicalis orthologue of the peroxidasin gene. We show that the predicted protein sequence of Xenopus peroxidasin shows high sequence identity with the human orthologue and that the exon structure is highly conserved between the two species. We describe the first detailed developmental expression pattern for peroxidasin in a vertebrate species. Maternal expression of Xtpxn is localized to the animal hemisphere where it persists through early cleavage stages. Initial zygotic Xtpxn expression is detected in the developing neural tube and becomes localized to the hindbrain and midbrain. Xtpxn is expressed in the primordium of the pronephric kidney and expression persists in the pronephric tubules and duct throughout development. Potential roles for peroxidasin during early vertebrate development are discussed.
Tindall
Andrew J
AJ
Area 11, Department of Biology, University of York, York, YO10 5YW, UK.
Pownall
Mary Elizabeth
ME
Morris
Ian D
ID
Isaacs
Harry V
HV
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm; D001798:Blood Proteins; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D047093:Eosinophil Major Basic Protein; D005091:Exons; D020224:Expressed Sequence Tags; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology*; D008636:Mesencephalon / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009035:Mothers; D009399:Nephrons / Q000196:embryology*; D009432:Neural Crest / Q000196:embryology*; D009474:Neurons / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D009195:Peroxidase / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D010544:Peroxidases; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011509:Proteoglycans; D017472:Receptors, Interleukin-1; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012249:Rhombencephalon / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014981:Xenopus; D000097423:Peroxidasin
D000951:Antigens, Neoplasm; D001798:Blood Proteins; D018076:DNA, Complementary; D047093:Eosinophil Major Basic Protein; D016326:Extracellular Matrix Proteins; D010455:Peptides; D009195:Peroxidase; D010544:Peroxidases; D011509:Proteoglycans; D017472:Receptors, Interleukin-1; D000097423:Peroxidasin; C074129:PRG2 protein, human; C535215:PXDN protein, human