title: Diagnosis of a mixed mycoplasma infection associated with a severe outbreak of bovine pinkeye in young calves.
abstract: Mycoplasma bovoculi and Mycoplasma bovis were both isolated from conjunctival swabs taken from young calves showing symptoms consistent with infectious bovine keratoconjunctivitis (pinkeye). No Moraxella spp. or other nonmycoplasma bacteria were isolated in association with this severe clinical outbreak. Based on laboratory tests and clinical observations, the first phase of the disease was likely pneumonic in nature, possibly caused by bovine respiratory syncytial virus and M. bovis. In the subsequent phase of the disease course, infection with both M. bovoculi and M. bovis resulted in ocular disease. A combination of microbiological, serological, and molecular diagnosticmethods was used to elucidate the etiology of the outbreak.
authors.affiliation: Kimron Veterinary Institute, Bet Dagan, Israel.
authors.forename: Sharon
authors.initials: S
authors.lastname: Levisohn
authors.forename: Shlomo
authors.initials: S
authors.lastname: Garazi
authors.forename: Irinna
authors.initials: I
authors.lastname: Gerchman
authors.forename: Jacob
authors.initials: J
authors.lastname: Brenner
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D003228:Conjunctiva / Q000382:microbiology; D004279:DNA, Viral / Q000302:isolation & purification; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007639:Keratoconjunctivitis, Infectious / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral