[Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis].
OBJECTIVE
To establish an in vitro model for the development of mouse spermatogenic cells into sperm by using the immunodefective mouse as the incubator.


METHODS
Tissue grafting was performed using testis from 1-2 days old Kun-ming mice as donor tissue and immunodefective mice as recipients; the expression of TESK1 mRNA in grafts was determined by RT-PCR and the spermatogenesis further observed with histological analysis of grafts.


RESULTS
Molecular biological and histological analyses showed that grafts post-grafting not only expressed TESK1 mRNA as in normal mouse testis, but also exhibited similarities in the structure of seminiferous tubules and component of spermatogenic cells, including sperms.


CONCLUSIONS
Spermatogenic cells heterotopically grafted in vitro could continuously grow and complete spermatogenesis and finally develop into sperm.
Yu
Jie
J
Laboratory of Male Reproduction, Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518036, China. yujie007@hotmail.com
Ye
Jing
J
Zhang
Fang-ting
FT
Wan
Hui-juan
HJ
Yin
Mei-jun
MJ
Fang
Jia-zhi
JZ
Gui
Yao-ting
YT
Cai
Zhi-ming
ZM
D000818:Animals; D000831:Animals, Newborn; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D023421:Models, Animal; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000378:metabolism / Q000637:transplantation*; D016042:Transplantation, Heterotopic
D012333:RNA, Messenger; C097507:testis-specific protein kinase 1; D017346:Protein Serine-Threonine Kinases