title: [Study of the methods of determining the isoflavones in herbs of Trifolium pratense extractive].
abstract: OBJECTIVE
  To study the methods of determining the amount of Isoflavones in red clover extractive.
  
  
  METHODS
  RP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.
  
  
  RESULTS
  After being water-solved, the four groups of flavoes elements satisfactorily separated, the amount of feeding in a range of 0.024-0.336 microg which has a good linear relationship with the peaks, and the total isoflavones determining results repetition, RSD=10.1%.
  
  
  CONCLUSIONS
  A simple, reliable and effective quality-control method is given.
authors.affiliation: Mianyang Teacher's College, Mianyang 621000, Sichuan, China. Biam-55@sohu.com
authors.forename: Qing-quan
authors.initials: QQ
authors.lastname: Bian
authors.forename: Zhen-ping
authors.initials: ZP
authors.lastname: Yang
authors.forename: Mei-zhong
authors.initials: MZ
authors.lastname: Ma
authors.forename: Song
authors.initials: S
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D029921:Trifolium / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C040641:genistin; C007768:formononetin; C013908:daidzin