[Impact of high-density lipoprotein on the apoptosis of endothelial cells induced by oxidized low-density lipoprotein].
OBJECTIVE
To investigate the impact of HDL on oxLDL-induced apoptosis of endothelial cells and relevant mechanisms.


METHODS
Human umbilical venous endothelial cells (ECV-304) were exposed to oxLDL or oxLDL + HDL. The apoptosis of the endothelial cells was checked by the Flow cytometry and DNA electrophoresis, while caspase-3 activity was meaured simultaneously.


RESULTS
The apoptosis rates induced by 100 microg/ml and 200 microg/ml of oxLDL were 28.40 + 5.30% and 34.7 +/- 4.64%, with caspase-3 activity of 166. 01 +/- 16.44 and 263.74 +/- 46.62 pmol/(min x mg pro), respectively. The DNA electrophoresis showed typical ladders. With exposure to HDL (200 mixrog/ml), the above apoptosis rates dropped to 8.06 +/- 2.35% and 9.40 +/- 2.58% respectively. There were no apoptotic ladders while the caspase-3 activities were significantly reduced (67.73 +/- 14.83 and 111.26 +/- 27.13 pmol/(min x mg pro).


CONCLUSIONS
HDL can significantly reduce the apoptosis of cultured endothelial cells induced by oxLDL. This effect may be mediated via suppression of caspase-3 activity.
Zhang
Qing
Q
Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zeng
Zhi
Z
Ren
Min
M
Chen
Yucheng
Y
Huang
Minghui
M
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases