title: Nitrate-dependent salicylate degradation by Pseudomonas butanovora under anaerobic conditions.
abstract: Nitrate-dependent salicylate degradation by the denitrifying Pseudomonas butanovora was investigated and the molar ratio of the cometabolism under anaerobic circumstances was determined. The bacterium was able to utilize salicylate as an electron donor for the reduction of nitrate. Salicylate was eliminated via catechol, which is degraded by means of catechol 2,3-oxygenases (meta-cleavage), forming 2-hydroxymuconic semialdehyde. The molar ratios of NO(3)(-)-N:salicylate existing during the experiment accorded well with the assumed 1:1 molar ratio. The tolerances of the growth, the salicylate degradation and the denitrification of P. butanovora to various heavy metal ions were also studied. Although the strain was tolerant to Pb(2+) and Cu(2+) up to 1 mM in complete medium, salicylate utilization took place only up to a concentration of 0.1 mM for both heavy metal ions. Of the heavy metal ions investigated, Cd(2+) (at a concentration of 0.05 mM) displayed the highest inhibitory effect on salicylate degradation by P. butanovora.
authors.affiliation: Bay Zoltán Foundation for Applied Research, Institute for Biotechnology, Derkovits fasor 2., Szeged H-6726, Hungary. kesser@bay.u-szeged.hu
authors.forename: Péter
authors.initials: P
authors.lastname: Kesseru
authors.forename: István
authors.initials: I
authors.lastname: Kiss
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Bihari
authors.forename: Károly
authors.initials: K
authors.lastname: Pál
authors.forename: Péter
authors.initials: P
authors.lastname: Portöro
authors.forename: Béla
authors.initials: B
authors.lastname: Polyák
mesh_terms: D000693:Anaerobiosis; D002396:Catechols / Q000737:chemistry / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D007218:Industrial Microbiology / Q000379:methods; D019216:Metals, Heavy / Q000378:metabolism / Q000494:pharmacology; D009566:Nitrates / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D012459:Salicylates / Q000378:metabolism*
chemical_list: D002396:Catechols; D019216:Metals, Heavy; D009566:Nitrates; D012459:Salicylates; D010105:Oxygenases; C034221:catechol