title: Dentinal tubule disinfection using three calcium hydroxide formulations.
abstract: The objective of this study was to determine the antibacterial efficacy of three calcium hydroxide (CH) formulations using an in vitro model of Enterococcus faecalis dentinal tubule infection. CH mixed with water (CH), CH mixed with iodine-potassium iodide (CH+IKI), and CH mixed with iodoform and silicone oil (Metapex) were tested. Human cylindrical dentin specimens infected with E. faecalis were filled with disinfectants and incubated for 1 week. Dentin powder samples collected with ISO 018 burs showed a statistically significant reduction in E. faecalis for all three experimental groups in comparison with untreated control specimens. Statistically significant differences were also found between the three experimental groups. Metapex was the most effective dentinal tubule disinfectant, followed by CH+IKI and then CH. Similar results were observed at greater dentin tubule depths (ISO 021 burs) with the exception that intracanal treatment with CH resulted in significantly higher numbers of E. faecalis in comparison with untreated control specimens.
authors.affiliation: Center for Molecular Microbiology, Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida 32610-0424, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Cwikla
authors.forename: Myriam
authors.initials: M
authors.lastname: Bélanger
authors.forename: Steeve
authors.initials: S
authors.lastname: Giguère
authors.forename: Ann
authors.initials: A
authors.lastname: Progulske-Fox
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Vertucci
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000737:chemistry / Q000494:pharmacology*; D003804:Dentin / Q000382:microbiology* / Q000648:ultrastructure; D013293:Enterococcus faecalis / Q000187:drug effects; D006801:Humans; D006847:Hydrocarbons, Iodinated; D017613:Iodine Compounds; D012388:Root Canal Irrigants / Q000737:chemistry / Q000494:pharmacology*; D012827:Silicone Oils
chemical_list: D000891:Anti-Infective Agents, Local; D006847:Hydrocarbons, Iodinated; D017613:Iodine Compounds; C498115:Metapex; D012388:Root Canal Irrigants; D012827:Silicone Oils; C439385:iodine potassium iodide; C010473:iodoform; D002126:Calcium Hydroxide