title: [Improvement of the assay for pre-S2 Ag/Ab detection and its application in different patients].
abstract: A new ELISA inhibition test for measuring serum Pre-S2Ab was developed to avoid the "double positive" phenomenon. One hundred and twenty samples with Pre-S2Ab negative were detected for Pre-S2Ab, the critical range for Pre-S2Ab in normal population was confirmed: a positive result was decided while the inhibition ration was over 50%, a negative result was decided while the inhibition ratio was less than 34%, and a dubious result was decided while the ratio was over 34% but less then 50%. At the same time, serum samples from sixty patients with chronic hepatitis B were detected for Pre-S2Ag and Pre-S2Ab, it showed that (1) the positive ratio of HBV DNA in patients with Pre-S2Ag positive was much higher than in patients with Pre-S2Ag negative (P < 0.01); (2) to the 46 patients with HBV DNA positive, Pre-S2Ag had a better relationship with HBV DNA than the HBeAg/Ab did (P < 0.01). Furthermore, to patients with acute hepatitis B, the presence of Pre-S2Ab was closely related to the prognosis of the disease, and the Pre-S2Ab assay could also be used to evaluate the preventive effect of HB vaccine containing Pre-S2Ag.
authors.affiliation: Institute of Hepatology, People's Hospital of Beijing Medical University, Beijing 100044.
authors.forename: W
authors.initials: W
authors.lastname: Tong
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Guan
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: P
authors.initials: P
authors.lastname: An
authors.forename: B
authors.initials: B
authors.lastname: Feng
authors.forename: Q
authors.initials: Q
authors.lastname: Tao
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D008297:Male; D011379:Prognosis; D011498:Protein Precursors / Q000032:analysis* / Q000276:immunology
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050807:presurface protein 2, hepatitis B surface antigen