title: Murine CD45+CD86+ cells isolated from para-aortic lymph nodes in an abortion-prone model.
abstract: The present study aims to address whether the analysis of CD45+CD86+ cells isolated from para-aortic lymph nodes (pLNs) is valuable in assessment of the status of local immunity at the murine feto-maternal interface. CBA/J x DBA/2 mice, virgin CBA/J mice, and CBA/J x BALB/c mice were used as an abortion-prone model (group A), nonpregnant controls (group N), and fertile controls (group F), respectively. The percentage of CD45+CD86+ cells in the CD45+ cell group (CD45+CD86+ percentage for short) and the absolute number of these cells were determined by means of flow cytometry (FCM), using mononuclear cells isolated from pLNs collected 5.5, 9.5, and 13.5 days post-coitum (dpc), respectively, and mononuclear cells isolated from placentas 13.5 dpc. To clarify the identity of these CD86+ cells, FCM was also performed with CD3, CD19, and DX5 as specific markers for murine T-cells, B-cells, and NK cells, respectively. Both resorption rate and absolute number of resorptions were significantly higher in group A (29.3%, 1.8+/-1.0) than in group F (4.8%, 0.3+/-0.5, P<0.001, respectively). Similarly, both cell percentage and absolute number of CD45+CD86+ cells in pLNs collected 13.5 dpc were significantly higher in group A than in group F (27.5+/-14.0% versus 12.3+/-7.1%, and 1362+/-687 versus 615+/-353, P=0.001, respectively). The CD45+CD86+ percentage was around 7.5% in nonpregnant CBA/J mice, similar to the 10.6% in CBA/JxDBA/2 mice 5.5 dpc, but had increased dramatically, to 23.9%, by 9.5 dpc (P<0.001 versus nonpregnant mice and P=0.002 versus CBA/JxDBA/2 mice 5.5 dpc), and remained at a higher level (27.5%) until 13.5 dpc. However, this trend was not observed in group F during pregnancy. The increased CD45+CD86+ percentage at day 9.5 of gestation, when resorption begins, may support the assumption that CD45+CD86+ cells play a role in the course of embryo resorption. Lymphocyte phenotypic analysis in the lymph nodes that drain the pregnant uterus may be helpful to assess the status of local immunity at the feto-maternal interface.
authors.affiliation: Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Yaoying
authors.initials: Y
authors.lastname: Zeng
authors.forename: Jingxian
authors.initials: J
authors.lastname: Zhao
authors.forename: Shan
authors.initials: S
authors.lastname: Zeng
authors.forename: Jintao
authors.initials: J
authors.lastname: Huang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Feng
authors.forename: Jingfang
authors.initials: J
authors.lastname: Di
authors.forename: Meiyi
authors.initials: M
authors.lastname: Zhan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D001011:Aorta / Q000166:cytology / Q000276:immunology; D051940:B7-2 Antigen; D020964:Embryo Loss / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008431:Maternal-Fetal Exchange / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D011237:Predictive Value of Tests; D011247:Pregnancy; D011379:Prognosis
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D008562:Membrane Glycoproteins; D017493:Leukocyte Common Antigens