[A strain of A/PR/8/34 (H1N1)-like virus isolated from men].
An influenza virus was isolated in May, 1991 from a child with acute respiratory disease. The identification results with serologic method have revealed that the isolate was A/PR/8/34 (H1N1)-like virus. The analyses of nucleotide sequences in HA1 domain showed that there were 12 different positions on nucleotide sequences between the isolate and the A/PR/8/34 (H1N1) virus. These differences induced substitutions of 4 amino acids in HA1 protein molecule of isolate as compared with that of A/PR/8/34 (H1N1) virus. The oligonucleotide analyses of viral genome revealed that there were 11 different points between isolate and A/PR/8/34 (H1N1) virus, e. g. 2 deleted and 9 inserted in genome of isolate as compared with A/PR/8/34 (H1N1) strain. Therefore, the isolate possibly derived from A/PR/8/34 (H1N1) virus by laboratory contamination could be ruled out. However, where did A/Guangdong/6/91 (H1N1) virus come from? This question will be settled by further study.
Guo
Y J
YJ
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Wang
M
M
Cheng
X
X
D000595:Amino Acid Sequence; D000818:Animals; D002675:Child, Preschool; D002681:China; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data
D019267:Hemagglutinin Glycoproteins, Influenza Virus