Detection of genetically modified organisms in foods by DNA amplification techniques.
In this article, the different DNA amplification techniques that are being used for detecting genetically modified organisms (GMOs) in foods are examined. This study intends to provide an updated overview (including works published till June 2002) on the principal applications of such techniques together with their main advantages and drawbacks in GMO detection in foods. Some relevant facts on sampling, DNA isolation, and DNA amplification methods are discussed. Moreover; these analytical protocols are discuissed from a quantitative point of view, including the newest investigations on multiplex detection of GMOs in foods and validation of methods.
García-Cañas
Virginia
V
Institute of Industrial Fermentations, CSIC, Madrid, Spain.
Cifuentes
Alejandro
A
González
Ramón
R
D018744:DNA, Plant / Q000032:analysis*; D005189:False Positive Reactions; D005504:Food Analysis; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018744:DNA, Plant