title: Generation of clinical-grade monocyte-derived dendritic cells using the CliniMACS system.
abstract: We describe the generation of monocyte-derived dendritic cells (MoDC) from leukapheresis products using the CliniMACS system from Miltenyi Biotec. In a clinical setting, the method turned out to be feasible for the generation of clinical-grade MoDC from patients with metastatic renal-cell carcinoma. MoDC generated with this system exhibited a fully mature phenotype as well as high migratory and T-cell stimulatory capacity.
authors.affiliation: Department of Urology, University of Innsbruck, Innsbruck, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Putz
authors.forename: Hubert
authors.initials: H
authors.lastname: Gander
authors.forename: Reinhold
authors.initials: R
authors.lastname: Ramoner
authors.forename: Claudia
authors.initials: C
authors.lastname: Zelle-Rieser
authors.forename: Andrea
authors.initials: A
authors.lastname: Rahm
authors.forename: Walter
authors.initials: W
authors.lastname: Nussbaumer
authors.forename: Georg
authors.initials: G
authors.lastname: Bartsch
authors.forename: Lorenz
authors.initials: L
authors.lastname: Höltl
authors.forename: Martin
authors.initials: M
authors.lastname: Thurnher
mesh_terms: D002454:Cell Differentiation; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D007937:Leukapheresis; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000378:metabolism
chemical_list: C515370:CCR7 protein, human; D054400:Receptors, CCR7; D019707:Receptors, Chemokine