[Comparison of five usual techniques for detection of methicillin-resistant Staphylococcus aureus].
The detection of methicillin-resistant Staphylococcus aureus in an usual and daily test in a microbiology laboratory. But its interpretation isn't easy because the resistance expression in vitro is so variable. The authors compare five methods about 100 clinical isolated strains. The results are: disk diffusion method using oxacillin 5 microg, on a NaCl 5% Mueller Hinton agar, 100 ml presentation (biorad): specificity 91%; disk diffusion method using oxacillin 5 microg, on a NaCl 5% Mueller Hinton agar, ready to use plate (AES): specificity 95%; the Oxascreen agar (BD): specificity 96%; the oxacillin cupule of ATB STAPH strip (ATB expression bioMerieux system) NaCl 2% semi solid Mueller Hinton agar medium: specificity 100% and disk diffusion method using cefoxitin 30 microg, on a Mueller Hinton agar plate (bioMerieux): specificity 100%. Therefore the cefoxitin disk diffusion method is very interesting to make better the difference between methicillin-resistant and methicillin-sensible S. aureus, in association with the ATB STAPH strip.
Gueudet
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Laboratoire bactériologie-C. Lemblé, centre hospitalier de Sélestat, 23, avenue Pasteur, BP 216, 67604 Sélestat, France. t-gueudet@biosphere.fr
Lemblé
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C
D002440:Cefoxitin / Q000494:pharmacology; D006801:Humans; D016106:Methicillin Resistance / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods; D010068:Oxacillin / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D002440:Cefoxitin; D010068:Oxacillin