title: Biomechanical properties of normal tendons, normal palmar aponeuroses and palmar aponeuroses from patients with Dupuytren's disease subjected to elastase and chondroitinase treatment.
abstract: Normal tendons, normal palmar aponeuroses and specimens from patients with Dupuytren's disease, namely apparently normal palmar aponeuroses and contracture bands were subjected to elastase and chondroitinase ABC digestion. Maximum Young's modulus, normalized hysteresis loop and residual elongation were determined before and after enzyme treatment. In normal tendons, normal and apparently normal palmar aponeuroses both normalized hysteresis loop and residual elongation increased significantly after elastase treatment, whereas the stiffness decreased. Normalized hysteresis loop and residual elongation display changes corresponding to the amount of digested elastin. The increased viscosity of untreated contracture bands containing less elastin, as compared to normal palmar aponeurosis, was not affected by elastase. Obviously, the elastic fibers in normal shape and distribution are responsible for maintaining an elastic status with a low viscous stress component. With the exception of contracture bands chondroitinase ABC caused a minor increase of residual elongation and as opposed to elastase a decrease of normalized hysteresis loop indicating an increased mobility of the tissue fibers.
authors.affiliation: Ludwig Boltzmann Institut für experimentelle plastische Chirurgie, Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Millesi
authors.forename: R
authors.initials: R
authors.lastname: Reihsner
authors.forename: G
authors.initials: G
authors.lastname: Hamilton
authors.forename: R
authors.initials: R
authors.lastname: Mallinger
authors.forename: E J
authors.initials: EJ
authors.lastname: Menzel
mesh_terms: D000818:Animals; D019765:Chondroitin ABC Lyase / Q000494:pharmacology; D004387:Dupuytren Contracture / Q000503:physiopathology*; D004547:Elastic Tissue / Q000187:drug effects / Q000502:physiology*; D004548:Elasticity / Q000187:drug effects; D004549:Elastin / Q000187:drug effects / Q000502:physiology*; D006225:Hand / Q000033:anatomy & histology / Q000502:physiology*; D006801:Humans; D010196:Pancreatic Elastase / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013710:Tendons / Q000187:drug effects / Q000502:physiology*; D013718:Tensile Strength / Q000187:drug effects / Q000502:physiology; D014783:Viscosity / Q000187:drug effects
chemical_list: D004549:Elastin; D010196:Pancreatic Elastase; D019765:Chondroitin ABC Lyase