Inhibition of fowlpox virus by an aqueous acetone extract from galls of Guiera senegalensis J. F. Gmel (Combretaceae).
An aqueous acetone extract from the galls of Guiera senegalensis was screened for in vitro antiviral activity against fowlpox virus (FPV). Cytopathic effect (CPE) inhibition and plaque inhibition assays were used to show presence of antiviral effects against FPV, whilst cytotoxicity assays established the relative safety of the extract for cells in vitro. Phytochemical analyses revealed the presence of phenolic compounds including flavonoids, tannins and anthocyanins as well as steroids and alkaloids. Thin-layer chromatographical (TLC) analysis also revealed the presence of quercitrin, quercetin, kaempferol, apigenin, rutin, gallic acid as well as unknown flavonoids and unknown phenolic acids. The antiviral effect of the extract was partially attributed to phenolic components including flavonoids.
Lamien
Charles E
CE
Laboratoire de Biochimie et Chimie appliquées, UFR/SVT, Université de Ouagadougou, BP 7021, Ouagadougou 03, Burkina Faso. celamien@hotmail.com
Meda
Aline
A
Mans
Janet
J
Romito
Marco
M
Nacoulma
Odile G
OG
Viljoen
Gerrit J
GJ
D000096:Acetone; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D002645:Chickens; D002855:Chromatography, Thin Layer; D029764:Combretaceae; D005587:Fowlpox virus / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010941:Plant Tumors; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D010936:Plant Extracts; D000096:Acetone