Marker gene transfer into human haemopoietic cells using a herpesvirus saimiri-based vector.
Herpesvirus-based gene therapy vectors offer an attractive alternative to retroviral vectors because of their episomal nature and ability to accommodate large transgenes. Saimiriine herpesvirus 2 (HVS) is a prototypical gamma-2 herpesvirus that can latently infect numerous different cell types. A cosmid-generated HVS vector in which transforming genes have been deleted and the marker gene encoding enhanced green fluorescent protein (HVS-GFP) has been incorporated was evaluated for its potential to transduce CD34+ haemopoietic progenitors selected from cord blood. Expression of GFP could subsequently be readily detected in cells of the erythroid lineage in both CFU-GEMM assays and liquid differentiation cultures. These results confirm the potential of HVS as a candidate vector for gene therapy applications using primitive haemopoietic cells and suggest that it may be applicable to disorders affecting cells of the erythroid lineage.
Doody
G M
GM
Molecular Medicine Unit, University of Leeds, Leeds LS9 7TF, UK.
Leek
J P
JP
Bali
A K
AK
Ensser
A
A
Markham
A F
AF
de Wynter
E A
EA
D018952:Antigens, CD34 / Q000276:immunology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D003114:Colony-Forming Units Assay; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006402:Hematologic Diseases / Q000628:therapy*; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics*; D006801:Humans; D014161:Transduction, Genetic / Q000379:methods*; D019076:Transgenes
D018952:Antigens, CD34; D049452:Green Fluorescent Proteins