Gene discovery in Boophilus microplus, the cattle tick: the transcriptomes of ovaries, salivary glands, and hemocytes.
The quest for new control strategies for ticks can profit from high throughput genomics. In order to identify genes that are involved in oogenesis and development, in defense, and in hematophagy, the transcriptomes of ovaries, hemocytes, and salivary glands from rapidly ingurgitating females, and of salivary glands from males of Boophilus microplus were PCR amplified, and the expressed sequence tags (EST) of random clones were mass sequenced. So far, more than 1,344 EST have been generated for these tissues, with approximately 30% novelty, depending on the the tissue studied. To date approximately 760 nucleotide sequences from B. microplus are deposited in the NCBI database. Mass sequencing of partial cDNAs of parasite genes can build up this scant database and rapidly generate a large quantity of useful information about potential targets for immunobiological or chemical control.
Santos
Isabel K F de Miranda
IK
Center for Genetic Resources and Biotechnology, Empresa Brasileira de Pesquisa Agropecuária, Brasília, DF, Brazil. imsantos@rpm.fmrp.usp.br
Valenzuela
Jesus G
JG
Ribeiro
José Marcos C
JM
de Castro
Marilia
M
Costa
Juliana Nardelli
JN
Costa
Ana Maria
AM
da Silva
Edson Ramiro
ER
Neto
Olavo Bilac Rego
OB
Rocha
Clarisse
C
Daffre
Sirlei
S
Ferreira
Beatriz R
BR
da Silva
João Santana
JS
Szabó
Matias Pablo
MP
Bechara
Gervasio Henrique
GH
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D018076:DNA, Complementary / Q000032:analysis*; D030541:Databases, Genetic; D005260:Female; D015723:Gene Library; D006434:Hemocytes; D008297:Male; D009866:Oogenesis / Q000235:genetics*; D010053:Ovary; D016133:Polymerase Chain Reaction; D012469:Salivary Glands; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000235:genetics* / Q000472:pathogenicity*
D018076:DNA, Complementary