Effects of obstructive jaundice on neutrophil production and acquisition of chemotactic activity in the bone marrow.
OBJECTIVE
Increased numbers and enhanced functions of peripheral neutrophils have been observed in obstructive jaundice. However, the effects of obstructive jaundice on the bone marrow, that is neutrophil production and acquisition of neutrophil chemotactic activity, have been poorly understood. In the present study, differentials of bone marrow cells and chemotactic activity of bone marrow neutrophils were evaluated in bile duct-obstructed rats.


METHODS
Male Wistar rats underwent either bile duct obstruction for 10 days or bile duct obstruction for 4 days followed by 6 days' internal biliary drainage. Differentials of peripheral blood and bone marrow cells were sequentially determined. Chemotactic activity of peripheral and bone marrow neutrophils was evaluated with a modified Boyden method using interleukin-8 (recombinant rat Gro-beta) as a chemoattractant.


RESULTS
Numbers of peripheral neutrophils significantly increased after bile duct obstruction. Significant increases in the myeloid/erythroid (M/E) ratio of bone marrow cells were observed after bile duct obstruction. The neutrophil proliferative pool (promyelocytes and myelocytes) increased initially, followed by an increased neutrophil storage pool (metamyelocytes, bands, and segmented neutrophils). The M/E ratio as well as the neutrophil proliferative and storage pools normalized after internal biliary drainage. Chemotactic activity was enhanced in both peripheral and bone marrow neutrophils after bile duct obstruction, and enhanced chemotaxis was alleviated with internal biliary drainage.


CONCLUSIONS
The present results strongly suggest the principal role of the bone marrow in increasing the number of neutrophils and their chemotactic activity during obstructive jaundice.
Shimatani
Masaaki
M
The Third Department of Internal Medicine, Kansai Medical University, Osaka, Japan. smtnmk@nifty.com
Tsuji
Kazuyuki
K
Aze
Yoshiya
Y
Yamamoto
Shin
S
Shibatani
Nobuyuki
N
Imai
Yoshihito
Y
Takamido
Shoichiroh
S
Kubota
Yoshitsugu
Y
Okazaki
Kazuichi
K
D000818:Animals; D002634:Chemotaxis, Leukocyte; D041781:Jaundice, Obstructive / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors