[Random amplified polymorphic DNA analysis of the genomes among 7 species of ticks].
OBJECTIVE
To study genomic polymorphic DNA and genetic distance of 7 species of ticks.


METHODS
Ticks used in this study were Dermacentor nuttalli, D. silvarum, Haemaphysalis qinghaiensis, H. formosensis, H. punctata, Amblyomma testudinarium, and Ixodes ovatus. DNA extracts of the 7 species of ticks were amplified by random amplified polymorphic DNA (RAPD) and PCR technique using 5 primers with different arbitrary single chain polynucleotide sequences. DNA fingerprint maps were analyzed and the genetic distance among 7 species of ticks were counted.


RESULTS
The amplified products of the 7 species of ticks by RAPD all showed their specific DNA band. The average genetic distance among them was 0.71.


CONCLUSIONS
RAPD can differentiate the 7 species of ticks.
Yang
Yin-shu
YS
Military Medical Institute of Lanzhou Military Command, Lanzhou 730020, China.
Zhao
Hong-bin
HB
Di Wu
Jin-xue
JX
Zhang
Ji-jun
JJ
Shi
Zhi-yong
ZY
D000818:Animals; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D003870:Dermacentor / Q000145:classification / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000235:genetics; D018884:Ixodes / Q000145:classification / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000145:classification / Q000235:genetics*