[Effect of acute ethanol exposure on HO-1 enzyme activity in human primary hepatocytes].
OBJECTIVE
To study the effect of ethanol exposure on the expression of HO-1 enzymes in human primary culture hepatocytes.


METHODS
Human primary hepatocytes were isolated from human liver tissue and cultured in Williams' E medium. After ethanol exposure, HO-1 enzyme activity and protein level were detected by spectrophotometry and western blotting, respectively. Meanwhile, the level of aspartate transaminase (AST) in the supernatants and GSH content in hepatocytes were measured to indicate cell oxidative-damage.


RESULTS
There was remarkable dose- and time-dependent relationship between ethanol exposure and AST release, and a time-dependent decrease in GSH content. Moreover, we also observed that ethanol exposure could affect HO-1 enzyme activity and HO-1 protein level, at the beginning, it went up significantly then went down.


CONCLUSIONS
The increase of HO-1 enzyme activity and protein level after ethanol exposure may be relative to prevent against ethanol-induced oxidative injury in human primary hepatocytes.
Liu
Liegang
L
Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yao
Ping
P
Zhang
Xiping
X
Hao
Liping
L
Yang
Xuefeng
X
Sun
Xiufa
X
D001219:Aspartate Aminotransferases / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology; D006801:Humans
D000431:Ethanol; D051547:Heme Oxygenase-1; D001219:Aspartate Aminotransferases; D005978:Glutathione