[The active metabolite of leflunomide A771726 inhibits proliferation and collagen synthesis of hepatic stellate cell].
To investigate the effect of the metabolite of leflunomide, A771726,on proliferation and collagen synthesis of hepatic stellate cell (HSC).



HSC and Kupffer cells were isolated from the rat liver by collagenase IV and pronase perfusion, and purified by density gradient separation. The effects of A771726 on cell proliferation and collagen synthesis were examined by 3H-thymidine and 3H-proline incorporation assays, respectively. The TGF-beta, TNF-alpha and IL-1 levels in Kupffer cell conditioned medium (KCCM) were determined by enzyme-linked immunosorbent assay (ELISA).



HSC and Kupffer cells in rat liver were well separated. The KCCM of CCl4-injured rats had significant stimulation effect on proliferation and collagen synthesis of HSC in primary culture. Addition of A771726 (0.001-10 micromol/Lein HSC culture stimulated by KCCM significantly inhibited proliferation and collagen synthesis of HSC. Furthermore, the elevated TGF-beta, TNF-alpha and IL-1 levels in KCCM of CCl4-injured rats were significantly reduced in A771726 treatment groups.



A771726 has markedly inhibitory effect on proliferation and collagen synthesis of HSC and secretion of TGF-beta,TNF-alpha and IL-1 from Kupffer cells.
Yao
Hong-wei
HW
Zhejiang Respiratory Drugs Research Laboratory of State Food and Drugs Administration of China, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Li
Jun
J
Chen
Ji-qiang
JQ
Xu
Shu-yun
SY
D000814:Aniline Compounds / Q000494:pharmacology*; D000818:Animals; D002251:Carbon Tetrachloride; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D003437:Crotonates; D022781:Hepatocytes / Q000166:cytology*; D006885:Hydroxybutyrates / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism; D007555:Isoxazoles / Q000378:metabolism / Q000494:pharmacology*; D007728:Kupffer Cells / Q000166:cytology; D000077339:Leflunomide; D008297:Male; D009570:Nitriles; D051381:Rats; D017207:Rats, Sprague-Dawley; D014052:Toluidines; D016212:Transforming Growth Factor beta / Q000378:metabolism
D000814:Aniline Compounds; D024042:Collagen Type I; D003437:Crotonates; D006885:Hydroxybutyrates; D007166:Immunosuppressive Agents; D007375:Interleukin-1; D007555:Isoxazoles; D009570:Nitriles; D014052:Toluidines; D016212:Transforming Growth Factor beta; C527525:teriflunomide; D002251:Carbon Tetrachloride; D000077339:Leflunomide