title: IL-4 supports the generation of a dendritic cell subset from murine bone marrow with altered endocytosis capacity.
abstract: Dendritic cells (DC) of myeloid origin can be generated from mouse bone marrow (BM) using granulocyte macrophage-colony stimulating factor (GM-CSF). Immature major histocompatibility complex (MHC) II(low) DC are known to bear a high endocytosis capacity, in contrast to DC precursors and mature DC. Now we found that a subset of MHC II(low) DC in BM-DC cultures is unable to exert mannose receptor-mediated endocytosis of fluorescein isothiocyanate (FITC)-dextran (DX) and resembles immature Langerhans cells (LC). The FITC-DX endocytosis activity of LC-like cells occurs at an earlier stage of development, where the surface MHC II expression is absent or very weak. This LC-like subset expresses higher levels of E-cadherin but lower amounts of the markers Gr-1, scavenger receptor 2F8, and CD11b, when compared with the highly endocytic DC subset. The latter myeloid DC resemble monocyte-derived DC (MoDC). The sorted LC-like population develops completely and exclusively into mature MHC IIhigh DC, and the MoDC-like cells remain immature MHC II(low) DC or develop into adherent MHC IIneg macrophages or mature into MHC IIhigh DC. The development of LC-like cells is promoted by interleukin-4. Thus, we show here that the simultaneous development of LC-like and MoDC-like DC subsets occurs in standard bulk cultures with GM-CSF, suggesting the existence of two different precursors for LC and MoDC in BM.
authors.affiliation: Department of Dermatology, University Hospital Erlangen, Germany.
authors.forename: Mauritius
authors.initials: M
authors.lastname: Menges
authors.forename: Thomas
authors.initials: T
authors.lastname: Baumeister
authors.forename: Susanne
authors.initials: S
authors.lastname: Rössner
authors.forename: Patrizia
authors.initials: P
authors.lastname: Stoitzner
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Romani
authors.forename: André
authors.initials: A
authors.lastname: Gessner
authors.forename: Manfred B
authors.initials: MB
authors.lastname: Lutz
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000145:classification / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D015847:Interleukin-4 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II; D015847:Interleukin-4