Remorins form a novel family of coiled coil-forming oligomeric and filamentous proteins associated with apical, vascular and embryonic tissues in plants.
Remorins form a superfamily of plant-specific plasma membrane/lipid-raft-associated proteins of unknown structure and function. Using specific antibodies, we localized tomato remorin 1 to apical tissues, leaf primordia and vascular traces. The deduced remorin protein sequence contains a predicted coiled coil-domain, suggesting its participation in protein-protein interactions. Circular dichroism revealed that recombinant potato remorin contains an alpha-helical region that forms a functional coiled-coil domain. Electron microscopy of purified preparations of four different recombinant remorins, one from potato, two divergent isologs from tomato, and one from Arabidopsis thaliana , demonstrated that the proteins form highly similar filamentous structures. The diameters of the negatively-stained filaments ranged from 4.6-7.4 nm for potato remorin 1, 4.3-6.2 nm for tomato remorin 1, 5.7-7.5 nm for tomato remorin 2, and 5.7-8.0 nm for Arabidopsis Dbp. Highly polymerized remorin 1 was detected in glutaraldehyde-crosslinked tomato plasma membrane preparations and a population of the protein was immunolocalized in tomato root tips to structures associated with discrete regions of the plasma membrane.
Bariola
Pauline A
PA
Department of Plant Molecular Biology, University of Lausanne, Switzerland.
Retelska
Dorota
D
Stasiak
Andrzej
A
Kammerer
Richard A
RA
Fleming
Andrew
A
Hijri
Mohamed
M
Frank
Sabine
S
Farmer
Edward E
EE
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D002942:Circular Dichroism; D015151:Immunoblotting; D018551:Solanum lycopersicum / Q000737:chemistry / Q000235:genetics; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D018519:Meristem / Q000737:chemistry* / Q000235:genetics; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000032:analysis / Q000235:genetics; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D018520:Plant Shoots / Q000737:chemistry / Q000235:genetics; D010944:Plants / Q000737:chemistry* / Q000196:embryology / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis; D011198:Solanum tuberosum / Q000737:chemistry / Q000235:genetics
D002352:Carrier Proteins; D008565:Membrane Proteins; D009842:Oligopeptides; D010750:Phosphoproteins; D010940:Plant Proteins; D011994:Recombinant Proteins; C103794:remorin