Molecular identification of coproantigens of Cryptosporidium parvum compared to conventional staining techniques.
To compare the immunologic techniques to the conventional staining methods (mainly modified Ziehl Neelsen = MZN), 93 children (65 immunocompromised and 28 immunocompetent) potentially at risk of Cryptosporidium parvum were studied. Using MZN, a prevalence of 10.7% in diarrhoeic children was found. ELISA coproantigen and detection of 23 KDa band of immunoblotting by serum IgG were sensitive and specific. They gave 85.7% sensitivity, 100% specificity, 100% diagnostic accuracy, 100% positive predictive value and 98.9% negative predicttive value. ELISA detection of serum IgG gave 85.7% sensitivity, 97.7% specificity, 96.8% diagnostic accuracy, 75% positive predictive value and 98.9% negative predictive value. So, 23KDa band determined by ELISA is a valuable sensitive and specific mean of diagnosing cryptosporidiosis as this antigen is a consistent target of humoral immune response.
Abdel Baki
Mohamed H
MH
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
Younis
Tayser A H
TA
Habib
Khaled S
KS
Ramadan
Nashwa I
NI
Metwally
Doreya M
DM
Ismail
Khadiga A
KA
Saber
Maha M
MM
D000293:Adolescent; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G