Study of T-cell costimulatory blockade in vivo at a single-cell level.
The critical role of costimulatory signals in T-cell activation and the complexity of T-cell costimulatory pathways involved make a detailed understanding of this system a challenging task. By taking advantage of the unique chemical properties of CFSE, we and others have developed an in vivo model that allows quantitative analysis of T-cell activation at a single-cell level. This model involves labeling of donor T-cells with the tracking dye CFSE and adoptively transferring into lethally irradiated allogeneic hosts. T-cells proliferating in the host mice can be explicitly analyzed upon recovery. By using mice deficient for certain costimulatory molecules as a source of donor cells or by treating the host mice with reagents that block certain costimulatory pathways, this CFSE model is extremely useful in studying the role of T-cell costimulatory signals in activation, survival, and effector differentiation of alloreactive T-cells in vivo.
Vu
Minh Diem
MD
Division of Immunology, Department of Medicine, Harvard Medical School, Boston, MA, USA.
Li
Xian Chang
XC
D019264:Adoptive Transfer; D000818:Animals; D017690:Cell Transplantation; D005434:Flow Cytometry; D005452:Fluoresceins; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D013194:Staining and Labeling; D013388:Succinimides; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D014019:Tissue Donors; D014184:Transplantation, Homologous
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D013388:Succinimides