Activation of the oxidative burst by yeast elicitor in Catharanthus roseus cells occurs independently of the activation of genes involved in alkaloid biosynthesis.
In Catharanthus roseus cell suspensions, expression of several terpenoid indole alkaloid (TIA) biosynthetic genes, including those encoding strictosidine synthase and tryptophan decarboxylase, is coordinately induced by fungal elicitors such as yeast extract (YE). This induction is mediated by several signaling steps including the biosynthesis of jasmonic acid, and the activation of the jasmonic acid-responsive ORCA transcription factors. We investigated a possible role of reactive oxygen species (ROS) as a second messenger in this system. YE was shown to activate the production of ROS, which was dependent on protein phosphorylation and calcium influx. However, ROS generation was neither necessary for the induction of genes involved in TIA biosynthesis by YE nor by itself sufficient to induce these genes. Therefore, we conclude that activation of the oxidative burst by YE occurs independently of the activation of genes involved in TIA biosynthesis.
Pauw
Bea
B
Institute of Biology, Leiden University, Wassenaarseweg 64, Leiden, 2333 AL, The Netherlands.
van Duijn
Bert
B
Kijne
Jan W
JW
Memelink
Johan
J
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000494:pharmacology; D000111:Acetylcysteine / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D030008:Catharanthus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D005682:Gadolinium / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007811:Lanthanum / Q000494:pharmacology; D008954:Models, Biological; D009543:Nifedipine / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism*; D015003:Yeasts / Q000737:chemistry
D003470:Culture Media; D018749:RNA, Plant; D017382:Reactive Oxygen Species; D046948:Secologanin Tryptamine Alkaloids; C028521:lanthanum chloride; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D007811:Lanthanum; D005682:Gadolinium; D009543:Nifedipine; C038958:gadolinium chloride; D001205:Ascorbic Acid; D002118:Calcium; D000111:Acetylcysteine