Rapid and reliable identification of Staphylococcus equorum by a species-specific PCR assay targeting the sodA gene.
Rapid and reliable identification of Staphylococcus (S.) equorum was achieved by species-specific PCR assays. A set of primers targeting the manganese-dependent superoxide dismutase (sodA) gene of S. equorum was designed. Species-specificity of the primer set was evaluated by using a total of 112 strains (including 27 reference strains of the DSM collection), representing 26 different species of the genus Staphylococcus, 3 species of the genus Kocuria, and different strains of Macrococcus caseolyticus. By using primers SdAEqF and SdAEqR the expected PCR fragment was obtained only when DNA from S. equorum strains was used as template. The rapidity (about 4 h from DNA isolation to results) and reliability of the PCR procedures established suggests that the method may be profitably applied for specific detection and identification of S. equorum strains.
Blaiotta
Giuseppe
G
Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimentare, Ambientale e di Igiene, Stazione di Microbiologia Industriale, Università degli Studi di Napoli Federico II, Portici, Italy.
Ercolini
Danilo
D
Mauriello
Gianluigi
G
Salzano
Giovanni
G
Villani
Francesco
F
D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013482:Superoxide Dismutase / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase