title: In vitro clonal propagation of bird eye chilli (Capsicum frutescens Mill.).
abstract: An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl(-1) of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l(-1) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl(-1) activated charcoal (AC) were used for shoot regeneration from both shoot tip and nodal explants. Shoot tip explants (100%) grew well on medium containing 1 mgl(-1) of kinetin and 1 mgl(-1) of IBA. Shoot proliferation (1-3) from nodal explants was effective on this medium. The regenerated shoots with 4-7 nodes had further growth upon sub-culturing onto kinetin (1 mgl(-1)) and IBA (1 mgl(-1)) and rooted simultaneously. The rooted plants were transferred to pots after hardening under controlled conditions. The survival percentage in pots was 80-90%.
authors.affiliation: Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore, India.
authors.forename: H B
authors.initials: HB
authors.lastname: Gururaj
authors.forename: P
authors.initials: P
authors.lastname: Giridhar
authors.forename: Ashwani
authors.initials: A
authors.lastname: Sharma
authors.forename: B C N
authors.initials: BC
authors.lastname: Prasad
authors.forename: G A
authors.initials: GA
authors.lastname: Ravishankar
mesh_terms: D002212:Capsicum / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media / Q000378:metabolism*; D066298:In Vitro Techniques; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D012038:Regeneration
chemical_list: D003470:Culture Media