title: Production of polyclonal antibodies in rabbits.
abstract: The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way.
authors.affiliation: Signal Transduction Laboratory, Cancer Research UK, London Research Institute Laboratories, London, UK.
authors.forename: David C
authors.initials: DC
authors.lastname: Hancock
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: O'Reilly
mesh_terms: D000818:Animals; D000906:Antibodies; D007114:Immunization / Q000379:methods*; D010455:Peptides; D011817:Rabbits
chemical_list: D000906:Antibodies; D010455:Peptides