Cloning and characterization of mouse disabled 2 interacting protein 2, a mouse orthologue of human NOSTRIN.
The mouse disabled 2 interacting protein 2 (mDaIP2) had been obtained through yeast two hybrid system. It consists of 506 amino acids and its calculated molecular weight is 57.7 kDa. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain interacts with the proline rich domain of mDab2 which had been identified to possess a transcriptional activation function. In RA-treated F9 teratocarcinoma cell, the mDaIP2 and mDab2 genes were differentially expressed in a RA-responsive manner and both were detected to localize in cytoplasm and nucleus. Homology search of all NCBI sequences indicated that the amino acid sequence of mDaIP2 shares 82% identity with human NOSTRIN which controls activity, trafficking, and targeting of nitric oxide synthase (eNos). The eNos was not detected in RA-treated F9 cell. These results suggest that mDaIP2 somehow functions in a different fashion from NOSTRIN in F9 cell differentiation and that its function may be concerted with that of mDab2.
Choi
Young-Joon
YJ
School of Life Sciences and Biotechnology, Korea university, 5-ka, Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
Cho
Si-Young
SY
Kim
Hyung-Wook
HW
Kim
Jung-Ah
JA
Bae
Sung-Ho
SH
Park
Sung-Soo
SS
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D019084:Fluorescent Antibody Technique, Indirect; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; D004268:DNA-Binding Proteins; C478773:Dab2 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; C470345:NOSTRIN protein, human; C495611:Nostrin protein, mouse; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase