[Determination of chemical constituents of essential oil from the fruit of Eucalyptus globulus by GC-MS].
OBJECTIVE
The chemical components of essential oil from fruit of Eucalyptus globulus were analyzed by GC-MS.


METHODS
The essential oil were extracted by steam distillation, then separated by capillary gas chromatography. The amount of the component from essential oil were determined by normalization methods. Chromatographic conditions were: capillary column DB-WAX (0.32 mm x 30 m, 0.25 microm) was used, column temperature: initial temperature at 40 degrees C for 3 min,ramping 5 C x min(-1) to 250 degrees C (holding for 10 min) the detector MS.


RESULTS
31 component from the fruit of E. globulus were identified, which accounted for over 93.7% of total volatile oil.


CONCLUSIONS
The methods is reliable, stabilize and can be applied to identify the volatile oil from the fruit of E. globulus.
Liu
Yu-ming
YM
School of Pharmacy, Second Military Medical University, Shanghai 200433.
Chai
Yi-feng
YF
Wu
Yu-tian
YT
Hu
Yue-ming
YM
Song
Guo-xing
GX
D003511:Cyclohexanols / Q000032:analysis; D000077591:Eucalyptol; D005052:Eucalyptus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000032:analysis; D013729:Terpenes / Q000032:analysis
D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D013729:Terpenes; C445364:cadinol; C024714:caryophyllene; D000077591:Eucalyptol