From worm to human: bioinformatics approaches to identify FOXO target genes.
Longevity regulatory genes include the Forkhead transcription factor FOXO, in addition to NAD-dependent histone deacetylase silent information regulator 2 (Sir2). The FOXO/DAF-16 family of transcription factors constitute an evolutionarily conserved subgroup within a larger family known as winged helix or Forkhead transcriptional regulators. Here we demonstrate how to identify FOXO target genes and their potential cis-regulatory binding sites in the promoters via bioinformatics approaches. These results provide new testable hypotheses for further experimental verifications.
Xuan
Zhenyu
Z
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
Zhang
Michael Q
MQ
D000818:Animals; D019295:Computational Biology / Q000379:methods; D051858:Forkhead Transcription Factors; D005786:Gene Expression Regulation / Q000235:genetics* / Q000502:physiology; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020218:Response Elements / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D051858:Forkhead Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors