title: Quinate oxidation in Gluconobacter oxydans IFO3244: purification and characterization of quinoprotein quinate dehydrogenase.
abstract: Quinoprotein quinate dehydrogenase (QDH) is a membrane-bound enzyme containing pyrroloquinoline quinone (PQQ) as the prosthetic group. QDH in Gluconobacter oxydans IFO3244 was found to be inducible by quinate and it is not constitutively expressed in the absence of quinate. The purification of holo-form of QDH to nearly homogeneity was achieved. The purified QDH appears to have two subunits of approximately 65 and 21 kDa, which could be the result of proteolysis of single polypeptide. Kinetic analysis indicated that the purified enzyme is much more specific to quinate than QDH from Acinetobacter calcoaceticus. The efficiency of the artificial electron acceptor was also determined.
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Alisa S
authors.initials: AS
authors.lastname: Vangnai
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Wanchai
authors.initials: W
authors.lastname: De-Eknamkul
authors.forename: Nozomi
authors.initials: N
authors.lastname: Yoshihara
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D020600:Gluconobacter oxydans / Q000201:enzymology* / Q000254:growth & development; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D045542:PQQ Cofactor / Q000378:metabolism; D011801:Quinic Acid / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011801:Quinic Acid; D045542:PQQ Cofactor; D000429:Alcohol Oxidoreductases; C022511:quinate dehydrogenase