title: Adrenergic modulation of cytokine release in bone marrow progenitor-derived macrophage following polymicrobial sepsis.
abstract: Catecholamines may impact on the pathophysiology of sepsis by attenuating proinflammatory cytokine and augmenting antiinflammatory cytokine production by macrophages. We tested this premise in bone marrow monocyte progenitor-derived macrophages. Polymicrobial sepsis was induced in mice through cecal ligation and puncture. ER-MP 12 monocyte progenitors were isolated and differentiated into macrophages in vitro 72 hr later. Lipopolysaccharide (LPS)-stimulated cytokine production was measured with and without epinephrine, IL-10 and anti-IL-10 antibody. Epinephrine significantly increased IL-10 production, but attenuated TNF-alpha release exclusively through beta2 adrenergic receptors, and is independent of IL-10 production. Together, these results suggest that epinephrine can promote a potent antiinflammatory response in sepsis.
authors.affiliation: Department of Physiology, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Ave, Maywood, IL 60153, USA.
authors.forename: Kuzhali
authors.initials: K
authors.lastname: Muthu
authors.forename: Jiangping
authors.initials: J
authors.lastname: Deng
authors.forename: Richard
authors.initials: R
authors.lastname: Gamelli
authors.forename: Ravi
authors.initials: R
authors.lastname: Shankar
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Jones
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001262:Atenolol / Q000494:pharmacology; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004347:Drug Interactions; D004731:Endotoxins / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004837:Epinephrine / Q000378:metabolism* / Q000494:pharmacology; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D007813:Laparotomy / Q000379:methods; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D023461:Myeloid Progenitor Cells / Q000187:drug effects / Q000502:physiology*; D011412:Propanolamines / Q000494:pharmacology; D011897:Random Allocation; D018805:Sepsis / Q000276:immunology / Q000503:physiopathology*
chemical_list: D000319:Adrenergic beta-Antagonists; D000906:Antibodies; D016207:Cytokines; D004731:Endotoxins; D008070:Lipopolysaccharides; D011412:Propanolamines; D016753:Interleukin-10; C026777:ICI 118551; D001262:Atenolol; D004837:Epinephrine