title: [Molecular cloning and expression of human PDGF-B chain mature peptide gene].
abstract: OBJECTIVE
  To acquire sufficient PDGF-BB protein and provide the basis for the further studies of its role on the fracture healing and trauma reconstruction and its clinical applications.
  
  
  METHODS
  Constructed the prokaryotic expression vector pQE-PDGF-B with the gene rearrangement technique, and the monomeric form of recombinant PDGF-B expressed in E. coli M15.
  
  
  RESULTS
  PDGF-B mature peptide gene was inserted into prokaryotic expression vector pQE30, which was confirmed by PCR, enzyme digestion and sequencing identification; the expressed products of pQE-PDGF-B in E. coli showed a single protein on SDS-PAGE, and their expression level was about 15% of the total bacterial protein. The molecular weight of the purified PDGF-B protein was about 15 KDs on SDS-PAGE.
  
  
  CONCLUSIONS
  The construction of recombinant plasmid and preparation of the monomeric protein of PDGF-B provides a solid foundation for further studying the function of PDGF-BB and producing biologically PDGF-BB protein.
authors.affiliation: Department of Spine and Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. chenjt99@sina.com
authors.forename: Jian-ting
authors.initials: JT
authors.lastname: Chen
authors.forename: Chun-lu
authors.initials: CL
authors.lastname: Yang
authors.forename: Fan
authors.initials: F
authors.lastname: Deng
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Tan
authors.forename: Huan-zhang
authors.initials: HZ
authors.lastname: Tang
authors.forename: Zhong-min
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Da-di
authors.initials: DD
authors.lastname: Jin
mesh_terms: D000077214:Becaplermin; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D066298:In Vitro Techniques; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
chemical_list: D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin