title: Saccharide sensing using gold and silver nanoparticles--a review.
abstract: We review new methodologies for glucose sensing from our laboratories based on the specific biological interactions between Con A, dextran-coated gold nanoparticles and glucose, and the interactions between dextran, glucose, and boronic-acid capped silver nanoparticles in solution. Our new approaches promise new tunable glucose sensing platforms. Dextran-coated gold nanoparticles were aggregated with the addition of Con A resulting in increase an in absorbance of nanoparticles at 650 nm, where the post-addition of glucose caused the dissociation of the aggregates and thus a decrease in the absorbance at 650 nm. The interaction of glucose and dextran with boronic acid-capped silver nanoparticles in solution resulted in enhanced luminescence intensity cumulatively due to surface-enhanced fluorescence and the decrease in absorbance at 400 nm, with an increase in absorbance at 640 nm. Lifetime measurements were used to distinguish the contribution from the surface-enhanced fluorescence. TEM was employed to assess the aggregation of nanoparticles.
authors.affiliation: Center for Fluorescence Spectroscopy, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
authors.forename: Kadir
authors.initials: K
authors.lastname: Aslan
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Lakowicz
authors.forename: Chris D
authors.initials: CD
authors.lastname: Geddes
mesh_terms: D000465:Algorithms; D015374:Biosensing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D007700:Kinetics; D008163:Luminescent Measurements; D046529:Microscopy, Electron, Transmission; D009005:Monosaccharides / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D012834:Silver / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D013053:Spectrophotometry; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D001897:Boronic Acids; D003911:Dextrans; D009005:Monosaccharides; D012996:Solutions; D003208:Concanavalin A; D012834:Silver; D006046:Gold; D005947:Glucose