title: Differential expression of TGF-beta isoforms during differentiation of HaCaT human keratinocyte cells: implication for the separate role in epidermal differentiation.
abstract: The three mammalian isoforms of transforming growth factor-beta(TGF-beta1, beta2, beta3) are potent regulators of cell growth, differentiation, and extracellular matrix deposition. To study their role in skin differentiation, we investigated the expression of TGF-beta isoforms on cell growth and differentiation induction of the human keratinocyte cell line, HaCaT by elevating the Ca(2+) concentration. An ELISA and RT-PCR assay revealed secreted TGF-beta 1 protein and TGF-beta 1 mRNA were increased during calcium-induced differentiation. In contrast, major differences were seen for TGF-beta 2 and TGF-beta 3 mRNA which were decreased during differentiation, but TGF-beta 2 and TGF-beta3 protein were not evident on an ELISA. These results suggest different functions for each TGF-beta isoforms in epidermal differentiation, such that TGF-beta 1 is associated with the more differentiated state, and TGF-beta 2 and TGF-beta 3 may be associated the more proliferated state.
authors.affiliation: Department of Dermatology, College of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Hang-Rae
authors.initials: HR
authors.lastname: Cho
authors.forename: Seok-Beom
authors.initials: SB
authors.lastname: Hong
authors.forename: Young Il
authors.initials: YI
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Nack-In
authors.initials: NI
authors.lastname: Kim
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology*; D020033:Protein Isoforms / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D016212:Transforming Growth Factor beta