Three- and four-way parallel factor (PARAFAC) analysis of photochemically induced excitation-emission kinetic fluorescence spectra.
Independently emerging fluorescence profiles of unknown, photochemically induced degradation products of several naturally non-fluorescent pesticides were monitored using single exposure excitation-emission fluorescence spectroscopy. Three-way parallel factor analysis (PARAFAC) was employed to uniquely resolve the pure fluorescent spectra of the overlapping photolysis products. The quantitative utility of EEM photolysis-based determinations was demonstrated by employing four-way PARAFAC models built from EEM time cubes of multiple fenvalerate samples. The 4-way PARAFAC models were then used to predict original pesticide concentrations resulting in conservative limit of detection and root mean square errors of calibration (RMSEC) of 3 microM each.
Nahorniak
Michelle L
ML
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, USA.
Cooper
Gary A
GA
Kim
Yoon-Chang
YC
Booksh
Karl S
KS
D002138:Calibration; D005163:Factor Analysis, Statistical; D010575:Pesticides / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis*
D010575:Pesticides; D014874:Water Pollutants, Chemical