Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography.
A simple one-step purification method, using expanded bed, ion-exchange chromatography, for the fractionation of nisin Z produced by Lactococcus lactis subsp. lactis A164 was developed. The highest dynamic binding capacity (0.92) of the adsorbent was obtained at a superficial velocity of 367 cm h(-1), resulting in approx. 2.7-fold bed expansion. The range of pH for the maximum adsorption was 3-4. The isocratic elution with 0.15 M NaCl led to approx. >90% recovery. Single-step purification of nisin Z from unclarified A164 culture broth resulted in 31-fold purification with a 90% yield.
Cheigh
Chan-Ick
CI
Department of Biotechnology, Yonsei University, Seoul 120-749, Korea.
Kook
Moo-Chang
MC
Kim
Seong-Bo
SB
Hong
Young-Ho
YH
Pyun
Yu-Ryang
YR
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D013294:Lactococcus lactis / Q000737:chemistry* / Q000254:growth & development; D009561:Nisin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*
D001426:Bacterial Proteins; D009561:Nisin; C071364:nisin Z