Andes virus stimulates interferon-inducible MxA protein expression in endothelial cells.
MxA is a cellular protein activated in cells treated with type I interferons. MxA protein is a member of the dynamin superfamily of large guanosine triphosphatases. A unique property of Mx1 GTPases is their antiviral activity against a wide range of RNA viruses. Replication of several hantavirus strains is inhibited in cells constitutively expressing MxA protein. We have found that Andes virus (ANDV) infection up regulates transcription of MxA RNA and expression of MxA protein in human endothelial cells in vitro. Activation of MxA gene expression requires virus replication. Up-regulation of MxA gene expression in hantavirus infected cells varies depending on the cell type. The degree of activation of MxA gene expression is inversely correlated with the efficiency of hantavirus replication. Additionally, MxA protein is co-localized with hantavirus nucleocapsid protein in infected cell. Our data suggest that MxA by interacting with the virus nucleocapsid protein inhibits production of new infectious virus particles.
Khaiboullina
Svetlana F
SF
Department of Microbiology and Immunology, University of Nevada, Reno, Reno, Nevada 89577, USA.
Rizvanov
Albert A
AA
Deyde
Varough M
VM
St Jeor
Stephen C
SC
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D006476:Orthohantavirus / Q000276:immunology*; D018778:Hantavirus Infections / Q000276:immunology / Q000378:metabolism; D006801:Humans; D064547:Myxovirus Resistance Proteins; D015854:Up-Regulation; D014709:Vero Cells; D014779:Virus Replication / Q000276:immunology
C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D019204:GTP-Binding Proteins