Cytomegalovirus (CMV) glycoprotein B genotype and CMV DNA load in the amniotic fluid of infected fetuses.
OBJECTIVE
To assess the value of both cytomegalovirus (CMV) DNA quantification in amniotic fluid (AF) and CMV glycoprotein B (gB) genotype as prognostic factors in CMV congenital infection.


METHODS
Quantification of CMV DNA was performed prospectively by real-time PCR and gB genotypes were analysed by gene sequencing analysis in the amniotic fluid of 42 fetuses infected by CMV. These were correlated with clinical data on fetal anomalies and outcome.


RESULTS
The proportion of severely symptomatic fetuses was similar in each gB genotype group. Median CMV DNA load was higher in the group of symptomatic fetuses but this difference was not significant and high and low viral loads were found in both groups.


CONCLUSIONS
Neither gB genotype nor CMV DNA load in AF correlate with the severity of CMV congenital infection and these markers are unlikely to prove useful for the management of congenital infection.
Picone
Olivier
O
Service de Gynécologie Obstétrique, Hôpital de Poissy-St-Germain, 10 rue du Champ Gaillard, 78300 Poissy, France.
Costa
Jean-Marc
JM
Leruez-Ville
Marianne
M
Ernault
Pauline
P
Olivi
Martine
M
Ville
Yves
Y
D000653:Amniotic Fluid / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000000981:diagnostic imaging / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005315:Fetal Diseases / Q000821:virology*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011379:Prognosis; D014463:Ultrasonography; D014759:Viral Envelope Proteins / Q000235:genetics*
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus