title: [Determination of concentration and encapsulation efficiency of silybin liposomes].
abstract: OBJECTIVE
  To determine the encapsulation efficiency of silybin liposomes.
  
  
  METHODS
  After Silybin liposomes solution was separated through sphadex G-100 column, eluent collected was determined by the first derivative UV spectrometry.
  
  
  RESULTS
  The calibration curve of Silybin was linear in the range of 0.5-30.0 mg x L(-1) (r = 0.9998). The average recovery was 101.7%. The relative standard deviation was 1.8%. The results showed that the encapsulation efficiency ranged from 65.1%-83.0% when the drug/phospholipid ratio varied from 0.02-0.06. The concentration of SLB in liposomes prepared by optimum technique was 760.4 mg x L(-1) (n = 4).
  
  
  CONCLUSIONS
  The method is accurate, simple and suitable for the quality control of Silybin liposomes.
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212001, Jiangsu, China.
authors.forename: Jiang-nan
authors.initials: JN
authors.lastname: Yu
authors.forename: Xi-ming
authors.initials: XM
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Gao
mesh_terms: D016503:Drug Delivery Systems; D008081:Liposomes / Q000737:chemistry; D020944:Silybum marianum / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D000077385:Silybin; D012838:Silymarin / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D008081:Liposomes; D012838:Silymarin; D000077385:Silybin