Glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline.
A sensitive and selective amperometric glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline (PANI) was investigated. Poly (m-phenylenediamine) (PMPD), which was employed as an anti-interferent barrier and a protective layer to platinum microparticles, was deposited onto platinum-modified PANI in the presence of glucose oxidase. The morphology of PANI, Pt/PANI and PMPD-GOD/Pt/PANI were investigated by scanning electron microscopy. The results show that PANI has a nano-fibrous morphology. The enzyme electrode exhibits excellent response performance to glucose with linear range from 2 x 10(-6) to 12 x 10(-3) M and fast response time within 7s. Due to the selective permeability of PMPD, the enzyme electrode also shows good anti-interference to uric acid and ascorbic acid. The Michaelis-Menten constant km and the maximum current density imax of the enzyme electrode were 9.34 x 10(-3) M and 917.43 microA cm(-2), respectively. Furthermore, this glucose biosensor also has good stability and reproducibility.
Zhou
Haihui
H
College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Chen
Hong
H
Luo
Shenglian
S
Chen
Jinhua
J
Wei
Wanzhi
W
Kuang
Yafei
Y
D000814:Aniline Compounds; D015374:Biosensing Techniques / Q000295:instrumentation*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D010655:Phenylenediamines; D010984:Platinum; D011073:Polyamines; D013997:Time Factors
D000814:Aniline Compounds; D010655:Phenylenediamines; D011073:Polyamines; C435915:poly(1,3-phenylenediamine); C416807:polyaniline; D010984:Platinum; D005949:Glucose Oxidase; D005947:Glucose