title: Different dietary fatty acids have dissimilar effects on activity and gene expression of mitochondrial tricarboxylate carrier in rat liver.
abstract: The tricarboxylate carrier (TCC), an integral protein of the mitochondrial inner membrane, transports mitochondrial acetyl-CoA into the cytosol, where lipogenesis occurs. We investigated in rat liver mitochondria the effect of diets enriched with saturated fatty acids (beef tallow, BT), monounsaturated fatty acids (olive oil, OO) or n-3 polyunsaturated fatty acids (fish oil, FO), respectively, on the activity and expression of TCC. TCC activity decreased, in parallel with TCC mRNA abundance, only upon FO-feeding. The TCC transcription rate, mRNA turnover and RNA processing indicated that FO administration regulates TCC gene at transcriptional and post-transcriptional steps, whereas BT- and OO-feeding do not seem to affect either TCC activity or gene expression.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Laboratorio di Biochimica, Università di Lecce, Via Prov. le Lecce-Monteroni, I-73100 Lecce, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Siculella
authors.forename: Simona
authors.initials: S
authors.lastname: Sabetta
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Damiano
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Giudetti
authors.forename: Gabriele Vincenzo
authors.initials: GV
authors.lastname: Gnoni
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D015153:Blotting, Western; D002352:Carrier Proteins / Q000187:drug effects / Q000378:metabolism*; D019343:Citric Acid / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D004041:Dietary Fats; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D012333:RNA, Messenger; C020723:citrate-binding transport protein; D019343:Citric Acid