title: Use of a monoclonal antibody against Babesia bovis merozoite surface antigen-2c for the development of a competitive ELISA test.
abstract: Bovine babesiosis caused by Babesia bovis is a disease that hampers the production of beef and dairy cattle in tropical and subtropical regions of the world. New diagnostic methods based on recombinant antigens constitute valuable biotechnological tools for the strategic control of this disease. We have developed a competitive enzyme-linked immunosorbent assay that includes a recombinant form of the merozoite surface antigen-2c and a novel monoclonal antibody against it. Preliminary results showed that this test is able to identify specific antibodies against B. bovis from experimentally and naturally infected cattle.
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Castelar, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Dominguez
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Zabal
authors.forename: Silvina
authors.initials: S
authors.lastname: Wilkowsky
authors.forename: Ignacio
authors.initials: I
authors.lastname: Echaide
authors.forename: Susana
authors.initials: S
authors.lastname: Torioni de Echaide
authors.forename: Gustavo
authors.initials: G
authors.lastname: Asenzo
authors.forename: Anabel
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Patricia
authors.initials: P
authors.lastname: Zamorano
authors.forename: Marisa
authors.initials: M
authors.lastname: Farber
authors.forename: Carlos
authors.initials: C
authors.lastname: Suarez
authors.forename: Monica
authors.initials: M
authors.lastname: Florin-Christensen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology*; D016793:Babesia bovis / Q000276:immunology* / Q000472:pathogenicity*; D001404:Babesiosis / Q000175:diagnosis* / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology*; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; C476548:MSA-2 protein, Babesia bovis; D008565:Membrane Proteins; D015800:Protozoan Proteins