Induction of nuclear factor-kappaB activation through TAK1 and NIK by diesel exhaust particles in L2 cell lines.
Diesel exhaust particles (DEPs) are known to induce allergic responses in airway epithelial cells, such as the production of various cytokines via nuclear factor-kappa B (NF-kappaB). However, the intracellular signal transduction pathways underlying this phenomenon have not been fully examined. This study showed that DEP induced NF-kappaB activity via transforming growth factor-beta activated kinase 1 (TAK1) and NF-kappaB-inducing kinase (NIK) in L2 rat lung epithelial cells. DEP induced the NF-kB dependent reporter activity approximately two- to three-fold in L2 cells. However, this effect was abolished by the expression of the dominant negative forms of TAK1 or NIK. Furthermore, it was shown that DEP induced TAK1 phosphorylation in the L2 cells. These results suggest that TAK1 and NIK are important mediators of DEP-induced NF-kappaB activation.
Yun
Young-Pil
YP
Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University of Korea, 62 Youido-dong, Youngdunpo-gu, Seoul 150-713, Republic of Korea.
Joo
Jin-Deok
JD
Lee
Joo-Yong
JY
Nam
Hae-Yun
HY
Kim
Young-Hoon
YH
Lee
Kweon-Haeng
KH
Lim
Cheol-Soo
CS
Kim
Hyung-Jung
HJ
Lim
Yong-Gul
YG
Lim
Young
Y
D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D010316:Particle Size; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity*; D000097782:NF-kappaB-Inducing Kinase; D000099151:MAP Kinase Kinase Kinase 7
D020930:MAP Kinase Kinase Kinases; D016328:NF-kappa B; D017346:Protein Serine-Threonine Kinases; D001335:Vehicle Emissions; D000097782:NF-kappaB-Inducing Kinase; D000099151:MAP Kinase Kinase Kinase 7