Recent advances in microemulsion electrokinetic chromatography.
Microemulsion electrokinetic chromatography (MEEKC) is an electrodriven separation technique. Separations are typically achieved using oil-in-water microemulsions, which are composed of nanometre-sized droplets of oil suspended in aqueous buffer. The oil droplets are coated in surfactant molecules and the system is stabilised by the addition of a short-chain alcohol cosurfactant. The novel use of water-in-oil microemulsions for MEEKC separations has also been investigated recently. This report summarises the different microemulsion types and compositions used to-date and their applications with a focus on recent papers (2002-2004). The effects of key operating variables (pH, surfactant, cosurfactant, oil phase, buffer, additives, temperature, organic modifier) and methodology techniques are described.
Marsh
Alex
A
GlaxoSmithKline R&D, New Frontiers Science Park South, Harlow, Essex, UK. alex.c.marsh@gsk.com
Clark
Brian
B
Broderick
Margo
M
Power
Joe
J
Donegan
Sheila
S
Altria
Kevin
K
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000639:trends*; D019075:Electrophoresis, Capillary / Q000379:methods; D004655:Emulsions / Q000737:chemistry*
D004655:Emulsions