Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea.
In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effacing Escherichia coli isolated from preweaned (38 isolates) and postweaned (21 isolates) pigs. The eae(beta) gene detected in 19 E. coli from preweaned pigs and 10 E. coli from postweaned pigs was found to be the most common subtype, followed by eae(gamma), eae(epsilon), and eae(zeta) genes. Subtypes were not determined for 7 E. coli isolates. No other subtype of the eae gene was detected in eae+ E. coli evaluated in this study.
Ha
Seung-Kwon
SK
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea.
Hong
Keumsuk
K
Choi
Changsun
C
Jung
Kwonil
K
Ha
Yoonchul
Y
Kim
Junghyun
J
Kim
Sung-Hoon
SH
Yoon
Byoungsu
B
Chae
Chanhee
C
D018829:Adhesins, Bacterial / Q000235:genetics*; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D018829:Adhesins, Bacterial; D017931:DNA Primers; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli