title: Homograft valve bank: our experience in valve banking.
abstract: BACKGROUND
  A cardiac homograft valve bank with cryopreservation facility was established at the Institute of Cardiovascular Diseases, Madras Medical Mission, Chennai in July 1995.
  
  
  RESULTS
  During the last 7 1/2 years of its existence, from July 1995 to March 2003, 588 hearts were processed. The valves harvested were 390 aortic, 400 pulmonary and 39 others including mitral valve, aortic conduits, pericardium etc.; 176 (29.9%) hearts were discarded for various reasons which included failure to sterilize, HBV, HIV, HCV, treponema pallidum hemagglutination test positivity, atheromatous/fatty streaks, incompetent valves, and dissection mistakes. The valves were sterilized using an antibiotic cocktail of vancomycin, amikacin, streptomycin, cefotaxime and amphotericin B in Hank's balanced salt solution. Of the 585 valves issued for clinical use, 247 were aortic, 323 pulmonary and 15 others (mitral valve, pericardium, conduits). Gram negative bacilli were the predominant contaminants from the hearts during the first half (July 1995 to February 1999) and gram positive organisms were the predominant contaminants during the later half (March 1999 to March 2003) of the study period. A variety of fungal contaminants like candida, aspergillus, penicillium and other fungi were also isolated from the homograft hearts at procurement. The valves were used most commonly for Rastelli procedure/right ventricular-pulmonary artery conduit (48.71%) followed by Ross procedure (23.41%). The other procedures were aortic valve replacement (6.15%), truncus repair (5.81%), unifocalization with conduit repair (6.49%), aortoplasty (0.512%), left ventricular-pulmonary artery conduit (0.512%), pulmonary valve replacement (0.512%), aneurysm repair (0.34%), Norwood repair (0.34%), mitral valve replacement (0.17%) and other procedures (7%).
  
  
  CONCLUSIONS
  We have established a viable and functioning cardiac homograft valve bank to suit Indian conditions and till date, have issued 585 homograft valves for clinical use.
authors.affiliation: Department of Cardiology, International Center for Cardiothoracic and Vascular Diseases, Chennai. drkmc@md3.vsnl.net.in
authors.forename: Susan
authors.initials: S
authors.lastname: Verghese
authors.forename: P
authors.initials: P
authors.lastname: Padmaja
authors.forename: B
authors.initials: B
authors.lastname: Sindhu
authors.forename: S J
authors.initials: SJ
authors.lastname: Elizabeth
authors.forename: Nancy
authors.initials: N
authors.lastname: Lesley
authors.forename: K M
authors.initials: KM
authors.lastname: Cherian
mesh_terms: D015925:Cryopreservation; D006350:Heart Valve Prosthesis / Q000382:microbiology; D006351:Heart Valves; D006801:Humans; D007194:India; D013242:Sterilization; D014015:Tissue Banks; D014184:Transplantation, Homologous