Hydrolytic enzyme activities of extracted humic substances during the vermicomposting of a lignocellulosic olive waste.
Humic substances and three hydrolytic enzymes (beta-glucosidase, phosphatase and urease) were extracted by neutral sodium pyrophosphate from an olive waste (dry olive cake), alone or mixed with municipal biosolids, during a nine month vermicomposting process. Easily degradable compounds decreased during the vermicomposting process because of microbial consumption. When municipal biosolids were added to dry olive cake, microbial activity increased and the amounts of compounds extracted by pyrophosphate were three times lower than olive cake alone. In both instances, beta-glucosidase, phosphatase and urease activities of the organic extracts either increased or remained the same after a nine month period of vermicomposting, thus suggesting that the humus enzyme complexes resisted microbial and earthworm attack. It is known that humus immobilised enzymes also remain active in soil environments, reactivating the nutrient cycles in soil. The use as amendments of vermicomposted olive cake, alone or when mixed with biosolids, could be a good alternative to reactivate the C, P and N-cycles in degraded soils for regeneration purposes.
Benitez
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Department of Agroecology and Plant Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, P.O. Box 419, 18080 Granada, Spain. emilio.benitez@eez.csic.es
Sainz
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H
Nogales
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R
D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D011756:Diphosphates / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006812:Humic Substances; D006868:Hydrolysis; D007220:Industrial Waste; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D000069463:Olive Oil; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry* / Q000378:metabolism; D014510:Urease / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001617:beta-Glucosidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D011756:Diphosphates; D004798:Enzymes; D006812:Humic Substances; D007220:Industrial Waste; D000069463:Olive Oil; D010938:Plant Oils; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D010744:Phosphoric Monoester Hydrolases; D001617:beta-Glucosidase; D014510:Urease