title: A genome-wide scan for quantitative trait loci linked to obesity phenotypes among West Africans.
abstract: OBJECTIVE
  To identify quantitative trait loci (QTL) for three obesity phenotypes: body mass index (BMI), fat mass (FM) and percent body fat (PBF) in West Africans with type 2 diabetes (T2DM).
  
  
  METHODS
  An affected sibling pair (ASP) design, in which both siblings had T2DM. Obesity was analyzed as a quantitative trait using a variance components approach.
  
  
  METHODS
  Sib-pairs affected with T2DM from the Africa America Diabetes Mellitus (AADM) study, comprising 321 sibling pairs and 36 half-sibling pairs.
  
  
  METHODS
  Weight was measured on an electronic scale to the nearest 0.1 kg, and height was measured with a stadiometer to the nearest 0.1 cm. Body composition was estimated using bioelectric impedance analysis (BIA). Genotyping was carried out at the Center for Inherited Disease Research (CIDR) with a panel of 390 trinucleotide and tetranucleotide repeats.
  
  
  RESULTS
  The obesity-related phenotype showing the strongest linkage evidence was PBF on chromosome 2 (LOD 3.30 at 72.6 cM, marker D2S739). Suggestive linkage to FM was found on chromosomes 2 (LOD 2.56 at 80.4 cM) and 5 (LOD 2.25 at 98 cM, marker D5S1725). The highest LOD score for BMI was 1.68 (chromosome 4, 113.8 cM). The areas of linkage for the three phenotypes showed some clustering as all three phenotypes were linked to the same regions of 2p13 and 5q14, and our study replicated linkage evidence for several regions previously reported in other studies.
  
  
  CONCLUSIONS
  We obtained evidence for several QTLs on chromosome 2, 4 and 5 to three obesity phenotypes. This study provides data on the genetics of obesity in populations that are currently under represented in the global effort directed at understanding the pathophysiology of excess adiposity in free living individuals.
authors.affiliation: National Human Genome Center, College of Medicine, Howard University, Washington, DC 20059, USA.
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: A A
authors.initials: AA
authors.lastname: Adeyemo
authors.forename: T
authors.initials: T
authors.lastname: Johnson
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.forename: A
authors.initials: A
authors.lastname: Amoah
authors.forename: S
authors.initials: S
authors.lastname: Owusu
authors.forename: J
authors.initials: J
authors.lastname: Acheampong
authors.forename: K
authors.initials: K
authors.lastname: Agyenim-Boateng
authors.forename: B A
authors.initials: BA
authors.lastname: Eghan
authors.forename: J
authors.initials: J
authors.lastname: Oli
authors.forename: G
authors.initials: G
authors.lastname: Okafor
authors.forename: F
authors.initials: F
authors.lastname: Abbiyesuku
authors.forename: G M
authors.initials: GM
authors.lastname: Dunston
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: Collins
authors.forename: C
authors.initials: C
authors.lastname: Rotimi
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000328:Adult; D000368:Aged; D000886:Anthropometry; D044383:Black People / Q000235:genetics*; D015992:Body Mass Index; D002874:Chromosome Mapping / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D015894:Genome, Human; D005869:Ghana; D006801:Humans; D008126:Lod Score; D008297:Male; D008875:Middle Aged; D009549:Nigeria; D009765:Obesity / Q000150:complications / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D040641:Quantitative Trait Loci