Bridging the gap between in vitro and in vivo toxicology testing.
In vitro systems for neurotoxicological studies can be useful for the investigation of events associated with pertubations of cellular and molecular targets that are similar to those in the intact animal. The toxicities of organophosphorus compounds, which inhibit esterases, and 1,2,3,6-tetrahydropyridine (MPTP), which depletes dopamine, can be studied in human neuroblastoma cells.
Ehrich
Marion
M
Laboratory for Neurotoxicity Studies, Virginia Tech, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442, USA.
D000110:Acetylcholinesterase / Q000378:metabolism; D023401:Animal Use Alternatives / Q000379:methods*; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D004298:Dopamine / Q000378:metabolism; D004950:Esterases / Q000037:antagonists & inhibitors; D006801:Humans; D066298:In Vitro Techniques; D020267:MPTP Poisoning / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009943:Organophosphorus Compounds / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*; D014407:Tumor Cells, Cultured; D016875:tau Proteins / Q000378:metabolism
D009943:Organophosphorus Compounds; D016875:tau Proteins; D004950:Esterases; D000110:Acetylcholinesterase; D004298:Dopamine