Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis.
We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong humoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant proteins. A similar response was elicited by both proteins when injected intraperitoneally in Atlantic salmon. The present data indicates that these proteins are good candidates to be used in formulations to study the protective immunity of salmon to infection by P. salnonis.
Wilhelm
Vivian
V
Fundación Ciencia para la Vida, Universidad Andrés Bello, Chile.
Morales
Carmen
C
Martínez
Rodrigo
R
Rosemblatt
Mario
M
Burzio
Luis O
LO
Valenzuela
Pablo D T
PD
D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005815:Genetic Code / Q000235:genetics*; D016695:Glycosyltransferases / Q000235:genetics*; D007111:Immunity, Cellular; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D044147:Piscirickettsiaceae / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D012474:Salmon / Q000276:immunology / Q000382:microbiology*; D033882:Transferrin-Binding Protein B / Q000235:genetics*
D008565:Membrane Proteins; D011994:Recombinant Proteins; D033882:Transferrin-Binding Protein B; D016695:Glycosyltransferases; C021815:murein transglycosylase