title: [Isolation of coltivirus from mosquitoes in Beijing and it's biological characteristics].
abstract: Two strains (Beijing 95-70 and 95-75) of coltivirus were isolated from mosquitoes collected in Beijing during summer-autumn in 1994. The viruses caused cytopathogenic effect on C6/36 cells but not on BHK-21 and Vero cells, and were not lethal for new born mice and three week old mice. The isolates were resistant to 5'-IdU and ether, but sensitive to acid pH 3.0. There were 12 segments RNA shown by polyacrylamide gel electrophoresis (PAGE) and PAGE profile of the two isolates were similar (6-6). The PAGE profile was also similar to that of the TRT2 strain isolated in 1991, but there were at least 8 segments slightly different between the new isolates and TRT2 in band migration distance. Tissue culture cross-neutralization test showed that the new isolates were antigenically related to TRT2 strain identified as coltivirus. Growth curve of the two isolates on C6/36 cells showed that virus titer began to rise at the first day after infection and continued to increase up to 7.0 Log TCID50 per 0.1 ml and maintained a high level until the 21st day after infection. It seems that the virus may cause persistent infection on C6/36 cell.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
authors.forename: S
authors.initials: S
authors.lastname: Tao
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002681:China; D018162:Coltivirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D009033:Culicidae / Q000821:virology*; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D051379:Mice; D012088:Reoviridae Infections / Q000276:immunology / Q000821:virology; D014774:Virulence
chemical_list: D000914:Antibodies, Viral