[Amplification and sequencing of the gene coding for mucin-like protein from Schistosoma japonicum].
OBJECTIVE
To amplify and sequence the partial gene coding for mucin-like protein from Chinese isolates of Schistosoma japonicum (SjMLP).


METHODS
The antigenic determinants of SmMLP were predicted by PCGENE software and specific oligonucleotide primers were designed and synthesized. Total RNA was isolated from adult worms of S. japonicum using Trizol reagent and the coding region gene of SjMLP was amplified by RT-PCR technique.


RESULTS
The coding region of SjMLP gene was specifically amplified by RT-PCR and the size of amplified fragment was 756 base pairs. The DNA sequence analysis result indicated that the coding sequence of the MLP was highly homologous between S. mansoni and S. japonicum.


CONCLUSIONS
The amplified fragment is consistent to the predicted one, providing a basis for cloning and further study on DNA immunization.
Liu
Yan
Y
Department of Parasitology, College of Basic Medicine, Nanhua University, Hengyang 421001, China.
Xiao
Jian-hua
JH
Liao
Li
L
Zeng
Gu-qing
GQ
Zhang
Yu-kuai
YK
Yang
Sheng
S
Liang
Yu
Y
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019444:Vaccines, DNA / Q000235:genetics
D000947:Antigens, Helminth; D009077:Mucins; D011506:Proteins; D019444:Vaccines, DNA