title: Keratocyte loss in corneal infection through apoptosis: a histologic study of 59 cases.
abstract: BACKGROUND
  Keratocyte loss by apoptosis following epithelial debridement is a well-recognized entity. In a study of corneal buttons obtained from patients of corneal ulcer undergoing therapeutic keratoplasty, we observed loss of keratocytes in the normal appearing corneal stroma, surrounding the zone of inflammation. Based on these observations, we hypothesized that the cell loss in the inflammatory free zone of corneal stroma is by apoptosis that could possibly be a non-specific host response, independent of the nature of infectious agent.
  
  
  METHODS
  To test our hypothesis, in this study, we performed Terminal deoxyribonucleotidyl transferase-mediated d-Uridine 5" triphosphate Nick End Labelling (TUNEL) staining on 59 corneal buttons from patients diagnosed as bacterial, fungal, viral and Acanthamoeba keratitis. The corneal sections were reviewed for morphologic changes in the epithelium, stroma, type, degree and depth of inflammation, loss of keratocytes in the surrounding stroma (posterior or peripheral). TUNEL positivity was evaluated in the corneal sections, both in the zone of inflammation as well as the surrounding stroma. A correlation was attempted between the keratocyte loss, histologic, microbiologic and clinical features.
  
  
  RESULTS
  The corneal tissues were from 59 patients aged between 16 years and 85 years (mean 46 years) and included fungal (22), viral (15), bacterial (14) and Acanthamoeba (8) keratitis. The morphological changes in corneal tissues noted were: epithelial ulceration (52, 88.1%), destruction of Bowman's layer (58, 99%), mild to moderate (28; 47.5%) to severe inflammation (31; 52.5%). Morphologic evidence of disappearance or reduced number of keratocytic nuclei in the corneal stroma was noted in 49 (83%) cases; while the TUNEL positive brown cells were identified in all cases 53/54 (98%), including cases of fungal (19), bacterial (14), viral (13), and Acanthamoeba keratitis. TUNEL staining was located mostly in the deeper stroma and in few cases the peripheral stroma. TUNEL positivity was also noted with the polymorphonuclear infiltrates and in few epithelial cells (10 of 59, 17%) cases, more with viral infections (6/10; 60%).
  
  
  CONCLUSIONS
  We report apoptotic cell death of keratocytes in the corneal stroma in infectious keratitis, a phenomenon independent of type of infectious agent. The inflammatory cells in the zone of inflammation also show evidence of apoptotic cell death. It could be speculated that the infective process possibly triggers keratocyte loss of the surrounding stroma by apoptosis, which could possibly be a protective phenomenon. It also suggests that necrotic cell death and apoptotic cell deaths could occur simultaneously in infective conditions of the cornea.
authors.affiliation: Ophthalmic Pathology Service, Brien Holden Eye Research centre, Hyderabad Eye Research Foundation, L,V, Prasad Eye Institute, Hyderabad. geeta@lvpei.org
authors.forename: Geeta K
authors.initials: GK
authors.lastname: Vemuganti
authors.forename: Kishore
authors.initials: K
authors.lastname: Reddy
authors.forename: Ghazala
authors.initials: G
authors.lastname: Iftekhar
authors.forename: Prashant
authors.initials: P
authors.lastname: Garg
authors.forename: Savitri
authors.initials: S
authors.lastname: Sharma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D003319:Corneal Stroma / Q000473:pathology*; D003320:Corneal Ulcer / Q000382:microbiology / Q000473:pathology* / Q000601:surgery; D015817:Eye Infections / Q000382:microbiology / Q000473:pathology* / Q000601:surgery; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D015948:Keratoplasty, Penetrating; D008875:Middle Aged