title: Characterization of an acid phosphatase from Lactobacillus pentosus: regulation and biochemical properties.
abstract: OBJECTIVE
  To screen for phosphatase and phytase activities in Lactobacillus isolated from diverse ecosystems and to determine the biochemical properties and the factors that regulate the synthesis of the enzyme responsible for these activities in the selected strain, Lactobacillus pentosus CECT 4023.
  
  
  RESULTS
  These activities were determined spectrophotometrically by using p-nitrophenyl phosphate and sodium phytate as substrates. They were maximal at the onset of the stationary phase of growth and repressed in the presence of high glucose concentration and inorganic phosphate. The enzyme responsible for these activities was an acid phosphatase (E.C.3.1.3.2.), with a molecular mass of 69 kDa. The activity was optimum at pH 5.0 and 50 degrees C. It hydrolysed mono-phosphorylated substrates and phytate, albeit at lower rates. It was inhibited by iodoacetic acid, phenyl-methylsulphonyl fluoride, di-sodium pyrophosphate and Ca+2 while activated by Co+2 and low concentrations of L-ascorbic acid and EDTA.
  
  
  CONCLUSIONS
  Lactobacillus pentosus CECT 4023 produces a nonspecific acid phosphatase that hydrolyses a number of mono-phosphorylated substrates and phytate.
  
  
  CONCLUSIONS
  The results suggest that the phosphatase from L. pentosus CECT 4023 could partly contribute to reduce the phosphorylation degree of phytate and its derivatives and, thereby, their anti-nutrient properties during fermentation processes.
authors.affiliation: Cereal Laboratory, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Valencia, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Palacios
authors.forename: M
authors.initials: M
authors.lastname: Haros
authors.forename: C M
authors.initials: CM
authors.lastname: Rosell
authors.forename: Y
authors.initials: Y
authors.lastname: Sanz
mesh_terms: D010832:6-Phytase / Q000032:analysis; D000135:Acid Phosphatase / Q000032:analysis*; D001431:Bacteriological Techniques; D005285:Fermentation; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000201:enzymology*; D013053:Spectrophotometry; D013696:Temperature
chemical_list: D000135:Acid Phosphatase; D010832:6-Phytase