Anti-proliferative effects of oridonin on SPC-A-1 cells and its mechanism of action.
Oridonin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. We investigated the anti-proliferative effect of oridonin on the lung cancer cell line SPC-A-1 and its mechanism of action. Growth inhibition was measured using a microculture tetrazolium assay and apoptosis was measured by several standard methods. Western blot analysis measured the expression of bcl-2 and bax proteins. Oridonin (> 28 micromol/l) inhibited the growth of SPC-A-1 cells and induced apoptosis. Marked morphological changes indicative of apoptosis were observed, especially in cells treated with oridonin for 48 - 60 h. Western blot analysis revealed downregulation of bcl-2 and upregulation of bax proteins following treatment with oridonin for 48 h. We conclude that oridonin demonstrated anti-proliferative and apoptosis-inducing effects on SPC-A-1 cells in vitro, and that changes in bcl-2 and bax protein levels may play an important role in its mechanism of action.
Liu
J J
JJ
Department of Haematology and Oncology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Huang
R W
RW
Lin
D J
DJ
Peng
J
J
Wu
X Y
XY
Pan
X L
XL
Li
M Q
MQ
Lin
Q
Q
D017209:Apoptosis; D006690:Bisbenzimidazole / Q000494:pharmacology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000494:pharmacology; D053938:DNA Fragmentation; D004224:Diterpenes / Q000494:pharmacology*; D045786:Diterpenes, Kaurane; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D006801:Humans; D037602:Isodon / Q000378:metabolism*; D008854:Microscopy, Electron; D010936:Plant Extracts / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D004396:Coloring Agents; D004224:Diterpenes; D045786:Diterpenes, Kaurane; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D013778:Tetrazolium Salts; D013844:Thiazoles; D051028:bcl-2-Associated X Protein; C011959:oridonin; C022616:thiazolyl blue; D006690:Bisbenzimidazole