title: A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides.
abstract: The application of recombinant antibodies in plant biology research is limited because plant researchers have minimal access to high-quality phage display libraries. Therefore, we constructed a library of 1.3 x 10(10) clones displaying human single-chain variable fragments (scFvs) that is available to the academic community. The scFvs selected from the library against a diverse set of plant proteins showed moderate to high antigen-binding affinity together with high specificity. Moreover, to optimize an scFv as immunodetection agent, two expression systems that allow efficient production and purification of bivalent scFv-Fc and scFv-CkappaZIP fusion proteins were integrated. We are convinced that this antibody platform will further stimulate applications of recombinant antibodies such as the diagnostic detection or immunomodulation of specific antigens in plants.
authors.affiliation: Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Technologiepark 927, B-9052 Gent, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Eeckhout
authors.forename: Annelies
authors.initials: A
authors.lastname: De Clercq
authors.forename: Eveline
authors.initials: E
authors.lastname: Van De Slijke
authors.forename: Jelle
authors.initials: J
authors.lastname: Van Leene
authors.forename: Hilde
authors.initials: H
authors.lastname: Stals
authors.forename: Peter
authors.initials: P
authors.lastname: Casteels
authors.forename: Geert
authors.initials: G
authors.lastname: Persiau
authors.forename: Dominique
authors.initials: D
authors.lastname: Vercammen
authors.forename: Frank
authors.initials: F
authors.lastname: Van Breusegem
authors.forename: Marc
authors.initials: M
authors.lastname: Zabeau
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Laurent
authors.initials: L
authors.lastname: Jespers
authors.forename: Ann
authors.initials: A
authors.lastname: Depicker
authors.forename: Geert
authors.initials: G
authors.lastname: De Jaeger
mesh_terms: D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D000915:Antibody Affinity / Q000235:genetics* / Q000276:immunology; D002352:Carrier Proteins / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D010455:Peptides / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013379:Substrate Specificity / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D010455:Peptides; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins