[Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry used to bacteria detection].
To investigate the influence variations, three culture media and different culture time were evaluated using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry for the rapid identification of eight strains of bacteria. These culture media are Colombia Blood Agar, Eosin Ethylene Blue Agar and Nutrition Agar. Results demonstrate that different culture media and culture time can influence the reproducibility of the bacteria fingerprint. It is suggested that appropriate culture media and culture time can enhance the accuracy of the bacteria detection using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.
Sun
Zongke
Z
Institute of Environment Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Zhang
Wei
W
Chen
Xiping
X
D001419:Bacteria / Q000145:classification* / Q000254:growth & development; D003470:Culture Media; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D003470:Culture Media