title: Air-liquid interface promotes invasive growth of laryngeal squamous cell carcinoma with or without hypoxia.
abstract: Air-liquid interface (ALI) is a microenvironment of aerodigestive tract. In our previous study, ALI promoted invasive growth of laryngeal squamous cell carcinoma (SCC); but its mechanism was unclear. Hypoxia is also related to cancer spread. Here we show that ALI with or without hypoxia accelerated invasive growth of laryngeal SCC cells, using collagen gel invasion assay. Submerged condition (SMC) without ALI did not induce the invasion with or without hypoxia. ALI enhanced expression of the following growth-, invasion-, and motility-related molecules in the cells with or without hypoxia more greatly than SMC: c-Met, Ras, mitogen-activated protein kinase cascade proteins (Raf-1, MEK-1, and ERK-1/2), matrix metalloproteinase-1, and filamin A. The data indicate that ALI promotes invasive growth of SCC cells by enhancement of the invasive growth-related molecules above, through mechanisms that differ from hypoxia, suggesting that ALI microenvironment should be taken into account for the study of cancer biology.
authors.affiliation: Department of Pathology and Biodefence, Faculty of Medicine, Saga University, Saga 849-8501, Japan. todas@post.saga-med.ac.jp
authors.forename: Shuji
authors.initials: S
authors.lastname: Toda
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Yamada
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Aoki
authors.forename: Akira
authors.initials: A
authors.lastname: Inokuchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Sugihara
mesh_terms: D000388:Air; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000503:physiopathology*; D018929:Cell Culture Techniques / Q000379:methods*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D009361:Neoplasm Invasiveness; D010100:Oxygen / Q000378:metabolism*; D012996:Solutions; D013499:Surface Properties
chemical_list: D012996:Solutions; D010100:Oxygen