Exopolysaccharide production by a new Halomonas strain CRSS isolated from saline lake Cape Russell in Antarctica growing on complex and defined media.
A haloalkalophilic Halomonas strain CRSS, isolated from salt sediments in Antarctica, produced exocellular polysaccharides (EPS) up to 2.9 g g(-1) dry cells. Acetate was the most efficient carbon source for EPS production. The composition of media strongly affected the nature of the polymers; a mannan and a xylo-mannan, were obtained when cells were grown on complex media. Acetate was the most efficient carbon source for EPS production and in presence of this substrate, a new polysaccharide, a fructo-glucan, was produced. The EPS fraction was composed by glucose, fructose, glucosamine and galactosamine in relative proportions of 1:0.7:0.3:trace.
Poli
Annarita
A
Istituto di Chimica Biomolecolare (ICB), CNR, Via Campi Flegrei 34, Comprensorio Olivetti, Pozzuoli (Na), 80078, Italy.
Moriello
Vincenzo Schiano
VS
Esposito
Enrico
E
Lama
Licia
L
Gambacorta
Agata
A
Nicolaus
Barbara
B
D000864:Antarctic Regions; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D020632:Halomonas / Q000145:classification* / Q000254:growth & development / Q000378:metabolism*; D008386:Marine Biology; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D013045:Species Specificity; D014871:Water Microbiology
D003470:Culture Media; D011135:Polysaccharides, Bacterial