title: Expression of JunB induced by X-rays in mice.
abstract: OBJECTIVE
  To explore JunB gene expression in spleen cells of mice after the whole body irradiation as well as in normal hematopoietic and leukemia cells in the primary culture after different dosages of X-ray irradiation.
  
  
  METHODS
  Spleen cells were isolated from the mice irradiated with 3 Gy X-rays. Primary cultured cells from mice were incubated in different intervals after X-irradiation at different dosages. Total RNA was extracted from the cells and the fluctuation of JunB mRNA level was assessed by the RNA ratio of JunB/beta-actin measured by quantitative Northern blot hybridization.
  
  
  RESULTS
  After the mice were exposed to 3 Gy X-rays irradiation, JunB expression in spleen cells was remarkably and rapidly increased, and reached its peak 0.5 h later in C3H/He mice and 1 h later in Balb/c mice. In the primary culture of normal spleen and leukemia cells, JunB mRNA levels increased 30 min after irradiation. The enhanced levels of JunB mRNA were returned to a normal level within 240 min after irradiation.
  
  
  CONCLUSIONS
  JunB gene is responsive to ionizing irradiation and is induced at immediate-early phase after the stimulation. This suggests that the JunB gene plays an important role in the early process of the cells against radiation.
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China. wanhong50@hotmail.com
authors.forename: Hong
authors.initials: H
authors.lastname: Wan
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishihara
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002478:Cells, Cultured; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects*; D016758:Genes, jun / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D012333:RNA, Messenger; D013154:Spleen / Q000528:radiation effects*
chemical_list: D000199:Actins; D012333:RNA, Messenger