HLA class II allele typing using DNA obtained from human fingernail clipping material.
Obtaining deoxyribonucleic acid (DNA), is the starting point for most genetic analysis. Nail material constitutes a source of DNA of easy access. This communication reports DNA extraction from fresh nail clipping material, by a combined extraction method, and its use in the typing of HLA-class II genes. Nail clipping material can be considered a convenient material for genetic analysis.
Cervantes
Jorge
J
Department of Virology, Kagoshima University, Faculty of Medicine, Japón. jcervantes@medscape.com
D000328:Adult; D004247:DNA / Q000302:isolation & purification*; D005802:Genes, MHC Class II; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008297:Male; D009262:Nails / Q000737:chemistry*
D004247:DNA