title: Iron availability from whey protein hydrogels: an in vitro study.
abstract: The influence of whey protein hydrogel microstructure, filamentous versus particulate, on iron delivery was studied under different conditions, including simulated gastrointestinal conditions. Experiments were initially conducted to determine the impact of pH and enzymes on iron release. The results show that different iron release profiles can be obtained from filamentous and particulate gels. Particulate gels released more iron than filamentous gels at acidic pH, but the opposite was observed at alkaline pH. In the presence of pepsin at pH 1.2 or pancreatin at pH 7.5, both gel types showed increased protein hydrolysis, but only filamentous gels showed increased iron release, suggesting that matrix structure plays an important role in iron delivery. A dissolution test was carried out under gastrointestinal conditions to mimic the in vivo dissolution process. Filamentous gel released most of its iron during the intestinal phase of a simulated digestion, hence protecting iron during its transit in the gastric zone. Absorption of iron by the Caco-2 system, used to estimate intestinal absorption, revealed that filamentous gels favored intracellular iron absorption. These results suggest that filamentous gels show promise as matrices for transporting iron and promoting its absorption and therefore should be of major interest in the development of innovative functional foods.
authors.affiliation: Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Pavillon Paul-Comtois, Québec, G1K 7P4, Canada.
authors.forename: Gabriel E
authors.initials: GE
authors.lastname: Remondetto
authors.forename: Erick
authors.initials: E
authors.lastname: Beyssac
authors.forename: Muriel
authors.initials: M
authors.lastname: Subirade
mesh_terms: D000042:Absorption; D001682:Biological Availability; D018938:Caco-2 Cells; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism / Q000493:pharmacokinetics*; D008894:Milk Proteins; D008954:Models, Biological; D000067816:Whey Proteins
chemical_list: D020100:Hydrogels; D008894:Milk Proteins; D000067816:Whey Proteins; D007501:Iron