[Cloning of cDNA fragments related to adventitious root formation from mango cotyledon section].
Two cut surfaces of mango cotyledon (distal and proximal cut surfaces) showed different capability of adventitious root formation, only proximal cut surface could be induced to form the roots and the distal cut surface did not. cDNA fragments related to adventitious root formation from the cut sections were isolated with suppressive subtractive hybridization. The forward substracted cDNA library was constructed using the cDNAs of distal (non-rooting) cut surface as driver and the cDNAs of proximal (rooting) cut surface as tester. Six positive clones were obtained by Virtual Northern blots. In this study, the putative up-regulated genes showed by sequence analysis were reported in mango for the first time, the deduced proteins among the positive clones were homologous to transporters, transcriptional regulators and enzymes.
Xiao
Jie-Ning
JN
The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Guangzhou 510275, China.
Huang
Xue-Lin
XL
Zhang
Yi-Shun
YS
Li
Yin
Y
Li
Xiao-Ju
XJ
D015152:Blotting, Northern; D003001:Cloning, Molecular; D018548:Cotyledon / Q000502:physiology*; D031022:Mangifera / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D018517:Plant Roots / Q000254:growth & development*