title: A comparison of cDNA, oligonucleotide, and Affymetrix GeneChip gene expression microarray platforms.
abstract: We have conducted a study to compare the variability in measured gene expression levels associated with three types of microarray platforms. Total RNA samples were obtained from liver tissue of four male mice, two each from inbred strains A/J and C57BL/6J. The same four samples were assayed on Affymetrix Mouse Genome Expression Set 430 GeneChips (MOE430A and MOE430B), spotted cDNA microarrays, and spotted oligonucleotide microarrays using eight arrays of each type. Variances associated with measurement error were observed to be comparable across all microarray platforms. The MOE430A GeneChips and cDNA arrays had higher precision across technical replicates than the MOE430B GeneChips and oligonucleotide arrays. The Affymetrix platform showed the greatest range in the magnitude of expression levels followed by the oligonucleotide arrays. We observed good concordance in both estimated expression level and statistical significance of common genes between the Affymetrix MOE430A GeneChip and the oligonucleotide arrays. Despite their apparently high precision, cDNA arrays showed poor concordance with other platforms.
authors.affiliation: The Jackson Laboratory, Bar Harbor, ME 04609, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Woo
authors.forename: Jason
authors.initials: J
authors.lastname: Affourtit
authors.forename: Sandra
authors.initials: S
authors.lastname: Daigle
authors.forename: Agnes
authors.initials: A
authors.lastname: Viale
authors.forename: Kevin
authors.initials: K
authors.lastname: Johnson
authors.forename: Jurgen
authors.initials: J
authors.lastname: Naggert
authors.forename: Gary
authors.initials: G
authors.lastname: Churchill
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018076:DNA, Complementary; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D018076:DNA, Complementary