title: Exhaustive genotyping of the CEM15 (APOBEC3G) gene and absence of association with AIDS progression in a French cohort.
abstract: CEM15 (or APOBEC3G) has recently been identified as an inhibitor of human immunodeficiency virus type 1 (HIV-1) replication in vitro. To evaluate the impact of its genetic variations on the progression of acquired immunodeficiency syndrome (AIDS), we have performed an extensive genetic analysis of CEM15. We have sequenced CEM15 in a cohort of 327 HIV-1-seropositive patients with extreme disease progression phenotypes--either slow progression or rapid progression--and in 446 healthy control subjects, all of white descent. We have identified 29 polymorphisms with allele frequencies >1%, 14 of which were newly characterized. There were no significant associations between the polymorphisms or haplotypes of CEM15 and a disease progression phenotype in our cohort.
authors.affiliation: Equipe Genomique, Bioinformatique et Pathologies du Systeme Immunitaire, INSERM EMI0355, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Do
authors.forename: Alexandre
authors.initials: A
authors.lastname: Vasilescu
authors.forename: Gora
authors.initials: G
authors.lastname: Diop
authors.forename: Thomas
authors.initials: T
authors.lastname: Hirtzig
authors.forename: Simon C
authors.initials: SC
authors.lastname: Heath
authors.forename: Cédric
authors.initials: C
authors.lastname: Coulonges
authors.forename: Jay
authors.initials: J
authors.lastname: Rappaport
authors.forename: Amu
authors.initials: A
authors.lastname: Therwath
authors.forename: Mark
authors.initials: M
authors.lastname: Lathrop
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Matsuda
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Zagury
mesh_terms: D000071480:APOBEC-3G Deaminase; D000163:Acquired Immunodeficiency Syndrome / Q000209:etiology / Q000235:genetics*; D000483:Alleles; D015331:Cohort Studies; D003564:Cytidine Deaminase; D018450:Disease Progression; D005787:Gene Frequency / Q000276:immunology; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006679:HIV Seropositivity; D006239:Haplotypes / Q000276:immunology*; D006801:Humans; D009700:Nucleoside Deaminases; D011506:Proteins / Q000235:genetics*; D012097:Repressor Proteins; D017421:Sequence Analysis
chemical_list: D011506:Proteins; D012097:Repressor Proteins; D009700:Nucleoside Deaminases; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase