[Studies on Smads at transcription level in liver fibrosis of mice with Schistosomiasis japonica].
OBJECTIVE
To study Smads involved in TGF-beta signal transduction at the transcription level during the development of liver fibrosis in BALB/c mice infected by Schistosoma japonicum.


METHODS
BALB/c mice infected with cercariae of S. japonicum were used as liver fibrosis models. Liver specimens were harvested at 8, 12, 16 and 24 weeks after infection and normal control were sacrificed at the 24th week. A part of the liver specimens were preserved for pathologic examination and the other part was frozen for the detection of mRNA level of Smad 2, Smad 3, Smad 4 and Smad 7.


RESULTS
The level of Smad 3 mRNA was significantly higher than that of control at the later stage, while the mRNA level of Smad 2 decreased at 12 and at 24 weeks, respectively. No significant difference in the mRNA level of Smad 4 and Smad 7 was observed between the infection group and the control.


CONCLUSIONS
Smad 3 may induce the development of liver fibrosis in mice infected by S. japonicum while Smad 2 may induce the development of liver fibrosis at early stage and inhibit it at later stage.
Zhang
Bin-bin
BB
Institute of Infectious Diseases, First Affiliated Hospital of School of Medicine, Zhejiang University, Hangzhou 31003, China.
Jiao
Yang-wen
YW
Cai
Wei-min
WM
Tao
Jun
J
Zheng
Min
M
Dong
Feng-qin
FQ
Liu
Rong-hua
RH
D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000209:etiology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012554:Schistosomiasis japonica / Q000150:complications* / Q000378:metabolism*; D051785:Smad Proteins; D051899:Smad2 Protein; D051900:Smad3 Protein; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics
D004268:DNA-Binding Proteins; D011506:Proteins; D012333:RNA, Messenger; D051785:Smad Proteins; D051899:Smad2 Protein; C497783:Smad2 protein, mouse; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D015534:Trans-Activators