title: siRNAdb: a database of siRNA sequences.
abstract: Short interfering RNAs (siRNAs) are a popular method for gene-knockdown, acting by degrading the target mRNA. Before performing experiments it is invaluable to locate and evaluate previous knockdown experiments for the gene of interest. The siRNA database provides a gene-centric view of siRNA experimental data, including siRNAs of known efficacy and siRNAs predicted to be of high efficacy by a combination of methods. Linked to these sequences is information such as siRNA thermodynamic properties and the potential for sequence-specific off-target effects. The database enables the user to evaluate an siRNA's potential for inhibition and non-specific effects. The database is available at http://siRNA.cgb.ki.se.
authors.affiliation: Center for Genomics and Bioinformatics, Karolinska Institutet, Berzelius väg 35, S-171 77 Stockholm, Sweden. alistair.chalk@cgb.ki.se
authors.forename: Alistair M
authors.initials: AM
authors.lastname: Chalk
authors.forename: Richard E
authors.initials: RE
authors.lastname: Warfinge
authors.forename: Patrick
authors.initials: P
authors.lastname: Georgii-Hemming
authors.forename: Erik L L
authors.initials: EL
authors.lastname: Sonnhammer
mesh_terms: D000818:Animals; D001483:Base Sequence; D030561:Databases, Nucleic Acid; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry*; D013816:Thermodynamics; D014584:User-Computer Interface
chemical_list: D034741:RNA, Small Interfering