Expression of SOCS3 mRNA in bone marrow cells from CML patients associated with cytogenetic response to IFN-alpha.
Previously, we have demonstrated that constitutive expression of suppressor of cytokine signaling-3 (SOCS3) affects the sensitivity of chronic myelogenous leukemia (CML) cell lines to interferon-alpha (IFN-alpha). In the present study, we analyzed the expression of SOCS3 mRNA in bone marrow cells from patients with CML at diagnosis, with the aid of real-time polymerase chain reaction. SOCS3 mRNA expression in bone marrow cells from CML patients who responded well to IFN-alpha therapy was significantly lower than that in cells from healthy volunteers and patients who were resistant to IFN-alpha therapy. Methylation of SOCS3 promoter was absent in bone marrow cells from all CML patients examined. These results indicate that the expression of SOCS3 mRNA is inversely associated with the sensitivity to IFN-alpha both in vitro and in vivo and that differences in SOCS3 mRNA expression are not due to the methylation status of SOCS3 promoters.
Takeuchi
Kazuto
K
First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan.
Sakai
Ikuya
I
Narumi
Hirosi
H
Yasukawa
Masaki
M
Kojima
Kensuke
K
Minamoto
Yoko
Y
Fujisaki
Tomoaki
T
Tanimoto
Kazushi
K
Hara
Masamichi
M
Numata
Akihiko
A
Gondo
Hisashi
H
Takahashi
Masuhiro
M
Fujii
Nobuharu
N
Masuda
Kozo
K
Fujita
Shigeru
S
D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000188:drug therapy* / Q000473:pathology*; D008745:Methylation; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects / Q000235:genetics*; D012097:Repressor Proteins / Q000032:analysis / Q000187:drug effects / Q000235:genetics*; D012680:Sensitivity and Specificity; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014157:Transcription Factors / Q000032:analysis / Q000187:drug effects / Q000235:genetics*
D016898:Interferon-alpha; D012333:RNA, Messenger; D012097:Repressor Proteins; C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014157:Transcription Factors