title: Effects of obstructive jaundice on neutrophil production and acquisition of chemotactic activity in the bone marrow.
abstract: OBJECTIVE
  Increased numbers and enhanced functions of peripheral neutrophils have been observed in obstructive jaundice. However, the effects of obstructive jaundice on the bone marrow, that is neutrophil production and acquisition of neutrophil chemotactic activity, have been poorly understood. In the present study, differentials of bone marrow cells and chemotactic activity of bone marrow neutrophils were evaluated in bile duct-obstructed rats.
  
  
  METHODS
  Male Wistar rats underwent either bile duct obstruction for 10 days or bile duct obstruction for 4 days followed by 6 days' internal biliary drainage. Differentials of peripheral blood and bone marrow cells were sequentially determined. Chemotactic activity of peripheral and bone marrow neutrophils was evaluated with a modified Boyden method using interleukin-8 (recombinant rat Gro-beta) as a chemoattractant.
  
  
  RESULTS
  Numbers of peripheral neutrophils significantly increased after bile duct obstruction. Significant increases in the myeloid/erythroid (M/E) ratio of bone marrow cells were observed after bile duct obstruction. The neutrophil proliferative pool (promyelocytes and myelocytes) increased initially, followed by an increased neutrophil storage pool (metamyelocytes, bands, and segmented neutrophils). The M/E ratio as well as the neutrophil proliferative and storage pools normalized after internal biliary drainage. Chemotactic activity was enhanced in both peripheral and bone marrow neutrophils after bile duct obstruction, and enhanced chemotaxis was alleviated with internal biliary drainage.
  
  
  CONCLUSIONS
  The present results strongly suggest the principal role of the bone marrow in increasing the number of neutrophils and their chemotactic activity during obstructive jaundice.
authors.affiliation: The Third Department of Internal Medicine, Kansai Medical University, Osaka, Japan. smtnmk@nifty.com
authors.forename: Masaaki
authors.initials: M
authors.lastname: Shimatani
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Tsuji
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Aze
authors.forename: Shin
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shibatani
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Imai
authors.forename: Shoichiroh
authors.initials: S
authors.lastname: Takamido
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Kubota
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Okazaki
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte; D041781:Jaundice, Obstructive / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors