title: [Effect of ginsenoside Rg1 on expression of p21, cyclin E and CDK2 in the process of cell senescence].
abstract: OBJECTIVE
  To explore the possible role of p21, cyclin E and cyclin-dependent kinase 2 (CDK2) in the protection of ginsenoside Rg1 against tert-butylhydroperoxide (t-BHP)-induced senescence in WI-38 cells.
  
  
  METHODS
  The cellular ultrastructure, cytometric assay and beta-galactosidase (beta-gal) cytochemistry staining were used to evaluate cell senescence. The levels of of p21, cyclin E and CDK2 protein were detected by Western blot.
  
  
  RESULTS
  Pretreatment with Rg1 significantly attenuated t-BHP-induced senescence in WI-38 cells. Simultaneously, compared with cells treated with t-BHP alone, Rg1 pretreatment markedly decreased the level of p21 protein and increased the levels of CDK2 and cyclin E.
  
  
  CONCLUSIONS
  p21, cyclin E and CDK2 may be involved in the process of ginsenoside Rg1 protection against t-BHP-induced senescence in WI-38 cells.
authors.affiliation: The Affiliated Union Hospital, Fujian Medical University, Fujian Institute of Geriatrics, Fuzhou 350001, China.
authors.forename: Chao-hui
authors.initials: CH
authors.lastname: Zhao
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Jin
authors.forename: Yuan-gui
authors.initials: YG
authors.lastname: Zhu
authors.forename: Guang-bin
authors.initials: GB
authors.lastname: Shi
authors.forename: Yu-qi
authors.initials: YQ
authors.lastname: Zeng
authors.forename: Yong-kun
authors.initials: YK
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Peng
mesh_terms: D042846:CDC2-CDC28 Kinases / Q000378:metabolism*; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects*; D019927:Cyclin E / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors
chemical_list: D019927:Cyclin E; D036145:Ginsenosides; D020122:tert-Butylhydroperoxide; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); C035054:ginsenoside Rg1