title: The production of protein-loaded microparticles by supercritical fluid enhanced mixing and spraying.
abstract: In this study, we use supercritical carbon dioxide as a processing medium for the fabrication of poly(DL-lactic acid) P(DLLA) microparticles that encapsulate a protein material. We have previously demonstrated that this polymer and a dry powder of a protein can be mixed under supercritical carbon dioxide conditions (above 31.1 degrees C and 73.8 bar) and that the protein component retains its biological activity. In this paper, we progress the work to demonstrate that the plasticized polymer and dry powder protein mixture can be sprayed to form solid polymer particles that encapsulate the protein. Particle size range is between 10 and 300 microm after spraying. Ribonuclease A and lysozyme were encapsulated in the polymer without significant loss of enzymatic activity. Biological assays of insulin and calcitonin confirm retention of activity after fabrication of the microparticles and release of the peptides/proteins.
authors.affiliation: Critical Pharmaceuticals, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Whitaker
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Hao
authors.forename: Owen R
authors.initials: OR
authors.lastname: Davies
authors.forename: Gulay
authors.initials: G
authors.lastname: Serhatkulu
authors.forename: Snow
authors.initials: S
authors.lastname: Stolnik-Trenkic
authors.forename: Steven M
authors.initials: SM
authors.lastname: Howdle
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Shakesheff
mesh_terms: D002116:Calcitonin / Q000008:administration & dosage; D002245:Carbon Dioxide; D016503:Drug Delivery Systems; D007328:Insulin / Q000008:administration & dosage; D019344:Lactic Acid / Q000008:administration & dosage*; D009113:Muramidase / Q000008:administration & dosage; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage*; D011506:Proteins / Q000008:administration & dosage*; D012259:Ribonuclease, Pancreatic / Q000008:administration & dosage
chemical_list: D007328:Insulin; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D002245:Carbon Dioxide; D019344:Lactic Acid; C033616:poly(lactide); D002116:Calcitonin; D012259:Ribonuclease, Pancreatic; D009113:Muramidase