[Role of transforming growth factor beta3 on amylase secretion of submandibular gland cells in rat].
OBJECTIVE
To investigate the role of transforming growth factor beta3 (TGF-beta3) on the amylase secretion of rat submandibular gland cells (RSGCs).


METHODS
The RSGCs were cultured and identified. The expressions of CK 8.13, S-100 and Vimentin in the RSGCs were examined by immunohistochemical staining. The experimental group was divided into 5 groups according to different concentrations of TGF-beta3 (0.5, 1.0, 5.0, 10.0 and 25.0 ng/ml) and no TGF-beta3 culture was used as control group. The effects of TGF-beta3 on the cell proliferation and amylase secretion were examined at the 24th, the 48th, the 72nd and the 96th hour. MTT colorimetric method was used to estimate vital force of culture cells. Amylase protein was assayed by auto-biochemistry equipment and Western blotting.


RESULTS
The RSGCs were stained positively for CK 8.13 and S-100, but negatively for Vimentin. There were no significant differences in absorbency between the experimental groups and the control group (P>0.05). Compared with the control group, TGF-beta3 at concentrations of 0. 5-10. 0 ng/ml significantly stimulated the amylase secretion of RSGCs after 72 and 96 hours (P<0.01). But high concentration of TGF-beta3 (25.0 ng/ml) showed no stimulation. Western blotting demonstrated that the cultured RSGCs and submandibular gland had the same band of amylase electrophoresis.


CONCLUSIONS
TGF-beta3 can stimulate RSGCs to differentiate and to secrete amylase, but TGF-beta3 has no effect on proliferation of RSGCs.
Zhu
Yaomin
Y
Department of Oral and Maxillofacial Surgery, Affiliated First Hospital of China Medical University, Shenyang Liaoning, PR China. zymin555@hotmail.com
Zhou
Qing
Q
Wang
Yuxin
Y
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053782:Transforming Growth Factor beta3
C509046:Tgfb3 protein, rat; D016212:Transforming Growth Factor beta; D053782:Transforming Growth Factor beta3; D000681:Amylases