A physical map of the chicken genome.
Strategies for assembling large, complex genomes have evolved to include a combination of whole-genome shotgun sequencing and hierarchal map-assisted sequencing. Whole-genome maps of all types can aid genome assemblies, generally starting with low-resolution cytogenetic maps and ending with the highest resolution of sequence. Fingerprint clone maps are based upon complete restriction enzyme digests of clones representative of the target genome, and ultimately comprise a near-contiguous path of clones across the genome. Such clone-based maps are used to validate sequence assembly order, supply long-range linking information for assembled sequences, anchor sequences to the genetic map and provide templates for closing gaps. Fingerprint maps are also a critical resource for subsequent functional genomic studies, because they provide a redundant and ordered sampling of the genome with clones. In an accompanying paper we describe the draft genome sequence of the chicken, Gallus gallus, the first species sequenced that is both a model organism and a global food source. Here we present a clone-based physical map of the chicken genome at 20-fold coverage, containing 260 contigs of overlapping clones. This map represents approximately 91% of the chicken genome and enables identification of chicken clones aligned to positions in other sequenced genomes.
Wallis
John W
JW
Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA.
Aerts
Jan
J
Groenen
Martien A M
MA
Crooijmans
Richard P M A
RP
Layman
Dan
D
Graves
Tina A
TA
Scheer
Debra E
DE
Kremitzki
Colin
C
Fedele
Mary J
MJ
Mudd
Nancy K
NK
Cardenas
Marco
M
Higginbotham
Jamey
J
Carter
Jason
J
McGrane
Rebecca
R
Gaige
Tony
T
Mead
Kelly
K
Walker
Jason
J
Albracht
Derek
D
Davito
Jonathan
J
Yang
Shiaw-Pyng
SP
Leong
Shin
S
Chinwalla
Asif
A
Sekhon
Mandeep
M
Wylie
Kristine
K
Dodgson
Jerry
J
Romanov
Michael N
MN
Cheng
Hans
H
de Jong
Pieter J
PJ
Osoegawa
Kazutoyo
K
Nefedov
Mikhail
M
Zhang
Hongbin
H
McPherson
John D
JD
Krzywinski
Martin
M
Schein
Jacquie
J
Hillier
Ladeana
L
Mardis
Elaine R
ER
Wilson
Richard K
RK
Warren
Wesley C
WC
D000818:Animals; D002645:Chickens / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D020451:Contig Mapping; D016172:DNA Fingerprinting; D008040:Genetic Linkage / Q000235:genetics; D016678:Genome; D023281:Genomics; D020161:Physical Chromosome Mapping; D016324:Sequence Tagged Sites