Entamoeba histolytica DNA methyltransferase (Ehmeth) is a nuclear matrix protein that binds EhMRS2, a DNA that includes a scaffold/matrix attachment region (S/MAR).
The protozoan parasite Entamoeba histolytica express a cytosine-5 DNA methyltransferase (Ehmeth) that belongs to the DNMT2 protein family. The biological function of members of this DNMT2 family is unknown. In the present study, we have demonstrated that Ehmeth is a nuclear matrix protein. Indeed, we showed by south-western analysis and yeast one-hybrid system that Ehmeth binds to EhMRS2, a DNA element which contains the eukaryotic consensus scaffold/matrix attachment regions (S/MAR) bipartite recognition sequences. S/MARs have been implicated in a variety of important functions, such as genome organization and gene expression. The methylation status of cytosine located within EhMRS2 was analyzed by bisulfite genomic sequencing. We observed the presence of methylated cytosine within the 3'-end of EhMRS2. These data provide the first evidence that a member of the DNMT2 family interacts with a S/MAR containing DNA element.
Banerjee
Sulagna
S
Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Calcutta 700054, India.
Fisher
Ohad
O
Lohia
Anuradha
A
Ankri
Serge
S
D000818:Animals; D026501:Blotting, Southwestern; D027682:Cation Transport Proteins / Q000235:genetics; D003596:Cytosine / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D019175:DNA Methylation; D016054:DNA, Protozoan / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology*; D045170:Matrix Attachment Regions; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism; D011485:Protein Binding; D013447:Sulfites / Q000378:metabolism
D027682:Cation Transport Proteins; D016054:DNA, Protozoan; D034521:Nuclear Matrix-Associated Proteins; D013447:Sulfites; D003596:Cytosine; D004248:DNA (Cytosine-5-)-Methyltransferases