Characterization of initiation of angiogenesis in early stages of prostate adenocarcinoma development and progression in a transgenic murine model.
OBJECTIVE
A novel prostate-specific protein of 94 amino acids (PSP94) promoter/enhancer-directed transgenic murine model of prostate cancer (CaP) was examined to determine the temporal relationship of angiogenesis with the development and progression of CaP.


METHODS
Immunohistochemistry and in situ hybridization were performed to determine the protein and mRNA expression of basic fibroblast growth factor, vascular endothelial growth factor, and microvessel formation at different stages of CaP development and progression.


RESULTS
Differential expression of basic fibroblast growth factor and vascular endothelial growth factor protein was found with the development and progression of CaP (P <0.0001 and P <0.0001, respectively). Angiogenesis was identified in CaP. mRNA expression of basic fibroblast growth factor and vascular endothelial growth factor was present from high-grade prostatic intraepithelial neoplasia to poorly differentiated CaP.


CONCLUSIONS
The PSP94 gene is conserved in humans and rodents; therefore, the PSP94 transgenic murine model of CaP provides a preclinical model to test such antiangiogenic or other chemopreventive therapies that may be predictive of human CaP.
Gabril
Manal
M
Department of Surgery, London Health Sciences Centre, University of Western Ontario, London, Ontario, Canada.
Xuan
Jim
J
Moussa
Madeleine
M
Dinney
Colin P N
CP
Chin
Joseph L
JL
Izawa
Jonathan I
JI
D000230:Adenocarcinoma / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009374:Neoplasms, Experimental; D009389:Neovascularization, Pathologic; D011471:Prostatic Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D029584:Prostatic Secretory Proteins / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D029584:Prostatic Secretory Proteins; D042461:Vascular Endothelial Growth Factor A; C043076:beta-microseminoprotein; D016222:Fibroblast Growth Factor 2