Exfoliation of Helicobacter pylori from gastric mucin by glycopolypeptides from buttermilk.
In the human stomach, Helicobacter pylori, an ulcer pathogenic bacterium, colonizes the gastric mucosal layer primarily. The ability of glycopolypeptides (GPP) prepared from buttermilk to exfoliate H. pylori bound to gastric mucin was investigated. The GPP were prepared from buttermilk by digestion with trypsin, papain, pancreatin, bromelain, or pepsin. Helicobacter pylori ATCC 43504T and 43579 adhered more strongly to all of the GPP tested than to whole buttermilk, the soluble fraction of buttermilk, gastric mucin prepared from mouse stomach, or commercial pig gastric mucin. The GPP digested with trypsin, papain, or pancreatin were significantly more adherent. When the GPP concentration was 10 mg/mL, bound H. pylori ATCC 43504T, 43579, and 5 clinical isolates were exfoliated markedly from immobilized porcine gastric mucin following treatment with GPP digested with trypsin or pancreatin. This ability of GPP did not correlate with sialic acid content, indicating that sialic acid content is not important in the exfoliation of this microorganism. Such an ability may depend on the structure or number of sugar chains, or the position of sialic acid. We conclude that GPP promote the exfoliation of H. pylori bound to gastric mucin and prevent the de novo adherence of this microorganism. As such, GPP are a promising food material for preventing H. pylori infection.
Matsumoto
M
M
Fundamental Research Laboratory, Kyodo Milk Industry Co. Ltd., Hirai, Hinode, Tokyo 190-0182, Japan. m-matumoto@meito.co.jp
Hara
K
K
Kimata
H
H
Benno
Y
Y
Shimamoto
C
C
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001963:Bromelains / Q000378:metabolism; D043302:Cultured Milk Products / Q000737:chemistry*; D005752:Gastric Mucins; D006020:Glycopeptides / Q000378:metabolism / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010194:Pancreatin / Q000378:metabolism; D010206:Papain / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D013552:Swine; D014357:Trypsin / Q000378:metabolism
D005752:Gastric Mucins; D006020:Glycopeptides; D010194:Pancreatin; D001963:Bromelains; D014357:Trypsin; D010206:Papain; D010434:Pepsin A