Validation of the postimplantation rat whole-embryo culture test in the international ECVAM validation study on three in vitro embryotoxicity tests.
A detailed report is presented on the performance of the postimplantation rat whole-embryo culture (WEC) test in a European Centre for the Validation of Alternative Methods (ECVAM)-sponsored formal validation study on three in vitro tests for embryotoxicity. Twenty coded test chemicals, classified as non-embryotoxic, weakly embryotoxic or strongly embryotoxic on the basis of their in vivo effects in animals and/or humans, were tested in four laboratories. The outcome showed that the WEC test can be considered to be a scientifically validated test, which is ready for consideration for use in assessing the embryotoxic potentials of chemicals for regulatory purposes.
Piersma
Aldert H
AH
National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
Genschow
Elke
E
Verhoef
Aart
A
Spanjersberg
Marielle Q I
MQ
Brown
Nigel A
NA
Brady
Madeleine
M
Burns
Angie
A
Clemann
Nicole
N
Seiler
Andrea
A
Spielmann
Horst
H
D000826:Animal Testing Alternatives; D000818:Animals; D016002:Discriminant Analysis; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects*; D047108:Embryonic Development; D005060:Europe; D007391:International Cooperation; D004364:Pharmaceutical Preparations / Q000145:classification; D011786:Quality Control; D051381:Rats; D015203:Reproducibility of Results; D018675:Toxicity Tests / Q000379:methods
D004364:Pharmaceutical Preparations