title: A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication.
abstract: Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SlNAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SlNAC1 resulted in a substantial increase in viral DNA accumulation. SlNAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SlNAC1 plays an important role in the process by which REn enhances TLCV replication.
authors.affiliation: Horticulture Unit, Commonwealth Scientific and Industrial Research Organization, Plant Industry, Glen Osmond, South Australia, 5064, Australia.
authors.forename: Luke A
authors.initials: LA
authors.lastname: Selth
authors.forename: Satish C
authors.initials: SC
authors.lastname: Dogra
authors.forename: M Saif
authors.initials: MS
authors.lastname: Rasheed
authors.forename: Helen
authors.initials: H
authors.lastname: Healy
authors.forename: John W
authors.initials: JW
authors.lastname: Randles
authors.forename: M Ali
authors.initials: MA
authors.lastname: Rezaian
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D010940:Plant Proteins; D014157:Transcription Factors; D014764:Viral Proteins