[Detection of hepatitis B virus (HBV) DNA in renal biopsied tissues from patients with HBV related glomerulonephritis using molecular biology techniques].
The purpose of this study was to determine the pathogenesis of hepatitis B virus (HBV) related glomerulonephritits. Measurement of HBV DNA was performed in paraffin-embedded renal biopsied tissues from 20 patients with HBV related glomerulonephritis by using in situ hybridization (ISH) with digoxigenin-labelled probe and in situ polymerase chain reaction (IS-PCR). HBV DNA staining was detected in 17 out of 20 patients (85%) with probe of full-length HBV DNA and in 14 out of 17 patients (82.35%) with a probe of S + C segments of HBV DNA. 17 out of 20 patients (85%) were also positive by IS-PCR assay. HBV DNA positive particles were diffusely distributed in glomerular capillary loops, mesangial areas, renal tube, and they were mainly in the presentation of cytoplasm-nucleus and nucleus. The distribution of HBsAg detected by immunohistochemical technique (LSAB assay) coincided with that of HBV DNA. It is suggested that renal lesions due to HBV are not only the results of immunologic response, but also the outcome of direct invasion of HBV to this organ. Our study provided new evidence on the molecular mechanism level for the research of the pathogenesis in HBV related glomerulonephritis.
Zhang
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Department of Nephrology, General Hospital of Jinan Command, Jinan 250031.
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005921:Glomerulonephritis / Q000473:pathology / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007668:Kidney / Q000473:pathology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D008967:Molecular Biology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D055815:Young Adult
D004279:DNA, Viral