[Modulation of intestinal mucosal inflammatory factors by curcumin in rats with colitis].
OBJECTIVE
To explore the mechanism of modulation of intestinal mucosal inflammatory factors by curcumin, the inhibitor of the transcriptional factor nuclear factor -kappaB (NF-kappaB), in rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis, and screen for a targeted therapeutic agent for treatment of inflammatory bowel disease (IBD).


METHODS
Rats with TNBS-induced colitis were fed with diet containing 2.0% curcumin (treatment group), 0.5% sulfasalazine (SASP, positive control group), and normal diet (model group and negative control group). Changes in colonic mucosal histological scores were evaluated and the cytokine mRNA expressions in the colonic tissue assessed by semiquantitative reverse transcriptional PCR (RT-PCR).


RESULTS
Treatment with curcumin ameliorated the histopathologic signs in rats with TNBS-induced intestinal inflammation. Curcumin and sulfasalazine obviously suppressed the high expression of proinflammatory cytokine interleukin (IL)-1beta mRNA and increased the low expression of IL-10 mRNA in the colonic mucosa. Expression of the anti-inflammatory cytokine IL-4 mRNA was detected in none of the groups.


CONCLUSIONS
Curcumin could modulate the expressions of IL-1beta and IL-10 mRNA in murine model of IBD, which suggests the potential of curcumin as a targeted therapeutic agent for IBD.
Jian
Yan-ting
YT
Institute of Digestive Disease of PLA, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. zhangyl@fimmu.com
Wang
Ji-de
JD
Mai
Guo-feng
GF
Zhang
Ya-li
YL
Lai
Zhuo-sheng
ZS
D000818:Animals; D003474:Curcumin / Q000494:pharmacology*; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000378:metabolism*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid
D007375:Interleukin-1; D016328:NF-kappa B; D012333:RNA, Messenger; D016753:Interleukin-10; D015847:Interleukin-4; D014302:Trinitrobenzenesulfonic Acid; D003474:Curcumin