DNA-polycation complexation and polyplex stability in the presence of competing polyanions.
Polyelectrolyte complex (polyplex) formation was studied by employing tapping mode atomic force microscopy (AFM) and an ethidium bromide fluorescence assay. The polycations chitosan and poly-L-lysine were used to compact DNA and the stability of the polyplexes was evaluated upon exposure to competing polyanions (alginate and xanthan). Furthermore, the relative preference of these polycations for DNA and the competing polyanion was investigated. The results showed that neither poly-L-lysine nor chitosan displayed any selectivity in binding to DNA relative to the competing polyanions, demonstrating the importance of electrostatics in the binding of a polycation to a polyanion. However, the ability of the polyanions to destabilize the DNA-polycation complexes depended on both the polyanion and the polycation employed, indicating that polymer-specific properties are also important for the complexation behavior and polyplex stability. Destabilization experiments further showed that annealing yielded complexes that were less prone to disruption upon subsequent exposure to alginate. Annealing experiments of plasmid DNA-chitosan complexes showed an increased fraction of rods following temperature treatment, indicating that the rods most likely are the more stable morphology for this system.
Danielsen
Signe
S
Biophysics and Medical Technology, Department of Physics, The Norwegian University of Science and Technology, NTNU, NO-7491 Trondheim, Norway.
Maurstad
Gjertrud
G
Stokke
Bjørn T
BT
D000464:Alginates / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D005453:Fluorescence; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010957:Plasmids / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
D000464:Alginates; D048271:Chitosan; D004247:DNA; D004996:Ethidium; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007364:Intercalating Agents; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine; D011108:Polymers