Increased interferon gamma production by peripheral blood mononuclear cells in response to stimulation of overexpressed disease-specific 9-O-acetylated sialoglycoconjugates in children suffering from acute lymphoblastic leukaemia.
Disease-specific over-expression of 9-O-acetylated sialoglycoconjugates (9-O-AcSGs) on peripheral blood mononuclear cells (PBMC) of children with acute lymphoblastic leukaemia (ALL, PBMC(ALL)) has been demonstrated using a lectin, Achatinin-H, with specificity towards 9-O-AcSAalpha2-6GalNAc. This study investigated the contributory role of 9-O-AcSGs induced on PBMC(ALL). Stimulation of PBMC(ALL) with Achatinin-H through 9-O-AcSGs led to a lymphoproliferative response with a significantly increased interferon-gamma (IFN-gamma) production when compared with unstimulated cells as demonstrated by enzyme-linked immunosorbent assay and mRNA expression. Under identical conditions, PBMC(ALL) ablated of O-acetylations did not respond to such stimulation. In summary, it may be concluded that stimulation of over-expressed 9-O-AcSGs regulate signalling for proliferation, leading to the release of IFN-gamma. Controlled expression of these molecules may be exploited as potential targets for therapy, promising beneficial effects to children with ALL.
Ghosh
Shyamasree
S
Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata, India.
Bandyopadhyay
Suman
S
Pal
Santanu
S
Das
Benubrata
B
Bhattacharya
Dilip K
DK
Mandal
Chitra
C
D000293:Adolescent; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D006001:Glycoconjugates / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000276:immunology*; D037102:Lectins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000276:immunology; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012794:Sialic Acids / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013268:Stimulation, Chemical
D006001:Glycoconjugates; D037102:Lectins; D012333:RNA, Messenger; D012794:Sialic Acids; C050704:achatinin(H); C025676:9-O-acetyl-N-acetylneuraminic acid; D007371:Interferon-gamma