[Determination of clenbuterol in biological samples by high performance liquid chromatography with in series ultraviolet and electrochemical detection].
A method for the determination of clenbuterol in animal tissues and fluid is described. After a three-step pretreatment, involving sample extract, liquid-liquid partition and purification on a LC-WCX column, the separation of clenbuterol from interfering compounds present in biological samples was performed on a Hypersil BDS C18 column by high performance chromatography with in series ultraviolet and electrochemical detection. The mobile phase was a mixture of methanol and 0.01 mol/L potassium chloride (45:55). The UV-absorption 244 nm and the electrochemical detection pulse mode +1.0 V were applied in series. The results showed that concentration of clenbuterol and peak areas achieved a nice linear relation. The limit of detection of clenbuterol in animal foods is 0.5 microg/kg. The mean recovery of clenbuterol spiked at 2.5, 2.0, 1.5, 0.5 microg/kg levels with chicken and liver samples were 42.2%-96.1%, RSD were from 12.4 to 19.7% (n = 7). With the described method, animal food samples and biological fluid (e.g. urine and blood) were analyzed with satisfaction.
Miao
Hong
H
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wu
Yongning
Y
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002976:Clenbuterol / Q000032:analysis*; D004563:Electrochemistry; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D002976:Clenbuterol