Neurite outgrowth-stimulating peptide derived from bovine kappa-casein.
We found a novel peptide that stimulates neurite outgrowth in Neuro-2a mouse neuroblastoma cells, from the pepsin-pancreatin digest of bovine kappa-casein. The amino acid sequence of this peptide is Phe-Leu-Pro-Tyr-Pro-Tyr (FLPYPY), corresponding to peptidic sequence 76-81 of bovine kappa-casein. The neurite outgrowth-stimulating activity of FLPYPY was seen over 10(-9) M. On the other hand, FLPYP and FLPYPYY, which corresponded to sequences 76-80 and 76-82 of bovine kappa-casein respectively, were ineffective.
Sakaguchi
Minoru
M
Laboratory of Cell Biology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. sakaguti@gly.oups.ac.jp
Ishikawa
Chieko
C
Nishimura
Tomoaki
T
Sugihara
Emi
E
Matsumura
Eiko
E
D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000494:pharmacology*; D002417:Cattle; D045744:Cell Line, Tumor; D051379:Mice; D009414:Nerve Growth Factors / Q000737:chemistry; D016501:Neurites / Q000187:drug effects*; D009447:Neuroblastoma / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology*
D002364:Caseins; D009414:Nerve Growth Factors; D010446:Peptide Fragments