Matrix-implanted laser desorption/ionization mass spectrometry.
The implantation of low-velocity massive gold clusters is shown to be a method of choice for homogeneous incorporation of a metallic matrix into the near-surface region of a solid biopolymer for subsequent laser desorption/ionization (LDI) MS analysis. Matrix implanted (MI)LDI spectra from cluster-implanted pure test peptide or tissue exhibit molecular ion peaks similar to those observed by matrix-assisted LDI. Moreover, the ion emission is very reproducible from any spot on the surface of these test samples. MILDI promises to be a powerful technique for mass spectrometric analysis of native biological samples as demonstrated by the first results on rat brain tissues.
Novikov
Alexey
A
Institut de Physique Nucléaire, CNRS-IN2P3, 91406 Orsay, France, Equipe Endotoxines, UMR 8619 du CNRS, IBBMC, Université de Paris-Sud, 91405 Orsay, France.
Caroff
Martine
M
Della-Negra
Serge
S
Lebeyec
Yvon
Y
Pautrat
Michèle
M
Schultz
J Albert
JA
Tempez
Agnès
A
Wang
Hay-Yan J
HY
Jackson
Shelley N
SN
Woods
Amina S
AS
D000818:Animals; D001921:Brain / Q000378:metabolism; D002138:Calibration; D006046:Gold / Q000737:chemistry; D007328:Insulin / Q000032:analysis / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
D007328:Insulin; D010743:Phospholipids; D006046:Gold