title: Cryopreserved sibling oocytes and intracytoplasmic sperm injection rescue unexpectedly poor fertilization in conventional in vitro fertilization.
abstract: OBJECTIVE
  To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle.
  
  
  METHODS
  The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed.
  
  
  RESULTS
  After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX.
  
  
  CONCLUSIONS
  In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.
authors.affiliation: Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, Taipei.
authors.forename: Shee-Uan
authors.initials: SU
authors.lastname: Chen
authors.forename: Yih-Ron
authors.initials: YR
authors.lastname: Lien
authors.forename: Li-Jung
authors.initials: LJ
authors.lastname: Chang
authors.forename: Yi-Yi
authors.initials: YY
authors.lastname: Tsai
authors.forename: Hong-Nerng
authors.initials: HN
authors.lastname: Ho
authors.forename: Yu-Shih
authors.initials: YS
authors.lastname: Yang
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008677:Metaphase; D009865:Oocytes; D011247:Pregnancy; D011256:Pregnancy Outcome; D035781:Siblings; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*