Crystallization of the Lys49 PLA2 homologue, myotoxin II, from the venom of Atropoides nummifer.
Myotoxin II, a Lys49 catalytically inactive phospholipase A(2) homologue from Atropoides nummifer venom, was purified, characterized and crystallized. The crystals belongs to the tetragonal system, space group P4(3)2(1)2, with unit cell parameters (a=b=68.66 and c=63.87 angstroms). Diffraction data were collected to a resolution of 2.32 angstroms. The crystal structure is currently being determined using molecular replacement techniques.
Watanabe
Leandra
L
Department of Physics, IBILCE/UNESP, R. Cristovão Colombo 2265, São José do Rio Preto, SP, CEP 15054-000, Brazil.
Gava
Lisandra M
LM
Angulo
Yamileth
Y
Lomonte
Bruno
B
Arni
Raghuvir K
RK
D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000737:chemistry*; D018360:Crystallography, X-Ray; D054501:Group II Phospholipases A2; D008239:Lysine / Q000737:chemistry*; D010741:Phospholipases A / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D054467:Phospholipases A2; D030162:Reptilian Proteins; D014961:X-Ray Diffraction
D003435:Crotalid Venoms; D030162:Reptilian Proteins; C406786:myotoxin II, Atropoides nummifer; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; D008239:Lysine