A novel multidrug resistance phenotype of bladder tumor cells grown on Matrigel or SIS gel.
We have previously shown that growth of bladder carcinoma cell lines onto matrices such as Matrigel and small intestinal submucosal (SIS) gel cause distinct changes in cellular morphology and motility. In these studies, we found that bladder cells grown on Matrigel showed increased resistance to either doxorubicin or mitomycin-C whereas growth of cells in SIS gel caused either significant increases or little difference in drug resistance, depending on both the cells and the drug. Finally, it was found that this altered drug sensitivity is reversible with a finite half-life and is likely due to altered drug accumulation and/or cell cycle kinetics.
Hurst
Robert E
RE
Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA.
Kamat
Chandrashekhar D
CD
Kyker
Kimberly D
KD
Green
Dixy E
DE
Ihnat
Michael A
MA
D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor / Q000187:drug effects*; D003094:Collagen; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology; D004338:Drug Combinations; D018432:Drug Resistance, Multiple / Q000502:physiology*; D004354:Drug Screening Assays, Antitumor; D005109:Extracellular Matrix; D006801:Humans; D007413:Intestinal Mucosa; D007797:Laminin; D016685:Mitomycin / Q000378:metabolism / Q000494:pharmacology; D010641:Phenotype; D011509:Proteoglycans; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D016685:Mitomycin; D004317:Doxorubicin; D003094:Collagen