Development of the larval ovary in the moth, Plodia interpunctella.
The morphogenesis of ovaries and the organization of germ cells within them were visualized during the larval stages of the moth, Plodia interpunctella. The germ cells were observed by utilizing confocal microscopy coupled with immuno-fluorescent staining for the alpha-crystallin protein 25 (alphaCP25). The alphaCP25 was previously shown to be specific to germ cells of pupae and adults, and this study shows that alphaCP25 is present in larval germ cells as well. A cluster of 28 germ cells that stain for alphaCP25 was found in the gonads of newly hatched first instar larvae. The founding germ cells became segregated into four clusters, most likely by somatic cell intrusion, around the beginning of the second instar. Division of the primary germ cells began by the end of the second instar and the formation of all cystoblasts appeared to be completed within the four ovarioles by the end of the third instar. Within the ovarioles of third instar larvae, the germ cells were organized with a distal cap of seven germ cells which was segregated from the majority of the germ cells. The main body of germ cells was arranged around a central germ cell-free core as a spiral. Divisions of the cystoblasts to form cystocyte clusters were nearly completed during the fourth (last) larval instar. These features suggest that the strategy to produce follicles in moths is fundamentally different from the fruitfly, Drosophila. It appears that during the initial stages of ovary development in P. interpunctella, the primary germ cells undergo stage-complete divisions that are completed prior to the onset of the next set of divisions, which results in a complete complement of follicles available by the time of adult eclosion, while in Drosophila the primary germ cell divisions are initiated in the adult stage, and follicles are produced individually as resources are available.
Beckemeyer
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EF
US Department of Agriculture, Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service, 1700 SW 23rd Drive, Gainesville, FL 32604, USA.
Shirk
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PD
D000818:Animals; D005260:Female; D019476:Insect Proteins / Q000032:analysis; D007814:Larva / Q000254:growth & development; D018613:Microscopy, Confocal; D009036:Moths / Q000254:growth & development*; D010053:Ovary / Q000166:cytology / Q000254:growth & development*; D010063:Ovum / Q000166:cytology* / Q000648:ultrastructure; D011679:Pupa
D019476:Insect Proteins