title: A physical map of the chicken genome.
abstract: Strategies for assembling large, complex genomes have evolved to include a combination of whole-genome shotgun sequencing and hierarchal map-assisted sequencing. Whole-genome maps of all types can aid genome assemblies, generally starting with low-resolution cytogenetic maps and ending with the highest resolution of sequence. Fingerprint clone maps are based upon complete restriction enzyme digests of clones representative of the target genome, and ultimately comprise a near-contiguous path of clones across the genome. Such clone-based maps are used to validate sequence assembly order, supply long-range linking information for assembled sequences, anchor sequences to the genetic map and provide templates for closing gaps. Fingerprint maps are also a critical resource for subsequent functional genomic studies, because they provide a redundant and ordered sampling of the genome with clones. In an accompanying paper we describe the draft genome sequence of the chicken, Gallus gallus, the first species sequenced that is both a model organism and a global food source. Here we present a clone-based physical map of the chicken genome at 20-fold coverage, containing 260 contigs of overlapping clones. This map represents approximately 91% of the chicken genome and enables identification of chicken clones aligned to positions in other sequenced genomes.
authors.affiliation: Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Wallis
authors.forename: Jan
authors.initials: J
authors.lastname: Aerts
authors.forename: Martien A M
authors.initials: MA
authors.lastname: Groenen
authors.forename: Richard P M A
authors.initials: RP
authors.lastname: Crooijmans
authors.forename: Dan
authors.initials: D
authors.lastname: Layman
authors.forename: Tina A
authors.initials: TA
authors.lastname: Graves
authors.forename: Debra E
authors.initials: DE
authors.lastname: Scheer
authors.forename: Colin
authors.initials: C
authors.lastname: Kremitzki
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Fedele
authors.forename: Nancy K
authors.initials: NK
authors.lastname: Mudd
authors.forename: Marco
authors.initials: M
authors.lastname: Cardenas
authors.forename: Jamey
authors.initials: J
authors.lastname: Higginbotham
authors.forename: Jason
authors.initials: J
authors.lastname: Carter
authors.forename: Rebecca
authors.initials: R
authors.lastname: McGrane
authors.forename: Tony
authors.initials: T
authors.lastname: Gaige
authors.forename: Kelly
authors.initials: K
authors.lastname: Mead
authors.forename: Jason
authors.initials: J
authors.lastname: Walker
authors.forename: Derek
authors.initials: D
authors.lastname: Albracht
authors.forename: Jonathan
authors.initials: J
authors.lastname: Davito
authors.forename: Shiaw-Pyng
authors.initials: SP
authors.lastname: Yang
authors.forename: Shin
authors.initials: S
authors.lastname: Leong
authors.forename: Asif
authors.initials: A
authors.lastname: Chinwalla
authors.forename: Mandeep
authors.initials: M
authors.lastname: Sekhon
authors.forename: Kristine
authors.initials: K
authors.lastname: Wylie
authors.forename: Jerry
authors.initials: J
authors.lastname: Dodgson
authors.forename: Michael N
authors.initials: MN
authors.lastname: Romanov
authors.forename: Hans
authors.initials: H
authors.lastname: Cheng
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: Kazutoyo
authors.initials: K
authors.lastname: Osoegawa
authors.forename: Mikhail
authors.initials: M
authors.lastname: Nefedov
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhang
authors.forename: John D
authors.initials: JD
authors.lastname: McPherson
authors.forename: Martin
authors.initials: M
authors.lastname: Krzywinski
authors.forename: Jacquie
authors.initials: J
authors.lastname: Schein
authors.forename: Ladeana
authors.initials: L
authors.lastname: Hillier
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Mardis
authors.forename: Richard K
authors.initials: RK
authors.lastname: Wilson
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Warren
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D020451:Contig Mapping; D016172:DNA Fingerprinting; D008040:Genetic Linkage / Q000235:genetics; D016678:Genome; D023281:Genomics; D020161:Physical Chromosome Mapping; D016324:Sequence Tagged Sites