[Effects of iso-osmotic salt stress on the activities of antioxidative enzymes, H+-ATPase and H+-PPase in tomato plants].
Seedlings of tomato (Lycopersicon esculentum Mill.) were grown in nutrient solution to investigate the changes in malondialdehyde (MDA) and proline content, and activities of antioxidative enzymes, plasmalemma H(+)-ATPase, and tonoplast H(+)-ATPase and H(+)-PPase to iso-osmotic stress of Ca(NO(3))(2) (80 mmol/L) and NaCl (120 mmol/L). The results indicated that both Ca(NO(3))(2) and NaCl stress significantly increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities in leaf cytosols and chloroplasts. The extent of increase caused by NaCl was higher than that by Ca(NO(3))(2). The activities of SOD, CAT and APX in mitochondria were increased under Ca(NO(3))(2) stress and decreased under NaCl stress. Salt stress enhanced the activities of plasmalemma H(+)-ATPase, tonoplast H(+)-ATPase and H(+)-PPase. The increases in tonoplast H(+)-ATPase and H(+)-PPase activity were much higher under Ca(NO(3))(2) stress than under NaCl stress. The MDA and proline content increased under salt stress, especially under NaCl stress. From the results, it can be concluded that both salt inhibited the plant growth, the effect of NaCl being much heavier.
Shi
Qing-Hua
QH
Key Laboratory of Horticultural Plant Development and Biotechnology, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310029, China.
Zhu
Zhu-Jun
ZJ
Khalid
Al-Aghabary
AA
Liu
Hui-Ying
HY
Yu
Jing-Quan
JQ
D060387:Ascorbate Peroxidases; D017610:Calcium Compounds / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000254:growth & development; D009566:Nitrates / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D017610:Calcium Compounds; D009566:Nitrates; D012965:Sodium Chloride; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D006180:Proton-Translocating ATPases; C059948:calcium nitrate