Ontogenesis and functional aspects of oxytocin and vasopressin gene expression in the thymus network.
Ontogenesis of oxytocin (OT) and vasopressin (VP) gene expression and function were investigated in murine thymus. OT and VP transcripts were detected in the thymus on embryonic days 13 and 15, respectively. Corresponding messenger RNAs were evidenced in thymic epithelial cells by in situ hybridization with a neurophysin probe. From all OT and VP receptors, only OTR was expressed by all T-cell subsets, while V1bR was found in double positive and single positive CD8 cells. In fetal thymic organ cultures, OTR antagonist d[D-Tyr(Et)2, Thr4]OVT increased early apoptosis of CD8 cells, while V1bR antagonist (Sanofi SSR149415) inhibited T-cell differentiation, and favored CD8 T-cell commitment.
Hansenne
I
I
Liege University Center of Immunology, Institute of Pathology, CHU-B23 Neuroendocrine-Immunology and Embryology, B-4000, Liege-Sart Tilman, Belgium.
Rasier
G
G
Péqueux
C
C
Brilot
F
F
Renard
Ch
Ch
Breton
C
C
Greimers
R
R
Legros
J-J
JJ
Geenen
V
V
Martens
H J
HJ
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D065092:Antidiuretic Hormone Receptor Antagonists; D015139:Blotting, Southern / Q000379:methods; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004622:Embryo, Mammalian; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods; D007211:Indoles / Q000494:pharmacology; D051379:Mice; D009481:Neurophysins / Q000235:genetics; D009924:Organ Culture Techniques; D010121:Oxytocin / Q000235:genetics / Q000378:metabolism*; D011759:Pyrrolidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D018045:Receptors, Oxytocin / Q000037:antagonists & inhibitors / Q000235:genetics; D017483:Receptors, Vasopressin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000145:classification / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D014667:Vasopressins / Q000235:genetics / Q000378:metabolism*; D014668:Vasotocin / Q000031:analogs & derivatives* / Q000494:pharmacology
C456029:1-(5-chloro-1-((2,4-dimethoxyphenyl)sulfonyl)-3-(2-methoxyphenyl)-2-oxo-2,3-dihydro-1H-indol-3-yl)-4-hydroxy-N,N-dimethyl-2-pyrrolidinecarboxamide; D065092:Antidiuretic Hormone Receptor Antagonists; D007211:Indoles; D009481:Neurophysins; D011759:Pyrrolidines; D012333:RNA, Messenger; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; C047046:atosiban; D014667:Vasopressins; D010121:Oxytocin; D014668:Vasotocin