title: In vitro susceptibilities of bloodstream isolates of Candida species to six antifungal agents: results from a population-based active surveillance programme, Barcelona, Spain, 2002-2003.
abstract: OBJECTIVE
  The antifungal drug susceptibilities of 351 isolates of Candida species, obtained through active laboratory-based surveillance in the period January 2002-December 2003, were determined (Candida albicans 51%, Candida parapsilosis 23%, Candida tropicalis 10%, Candida glabrata 9%, Candida krusei 4%).
  
  
  METHODS
  The MICs of amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole and caspofungin were established by means of the broth microdilution reference procedure of the European Committee on Antibiotic Susceptibility Testing.
  
  
  CONCLUSIONS
  Amphotericin B and flucytosine were active in vitro against all strains. A total of 24 isolates (6.8%) showed decreased susceptibility to fluconazole (MIC > or = 16 mg/L) and 43 (12.3%) showed decreased susceptibility to itraconazole (MIC > or = 0.25 mg/L). Voriconazole and caspofungin were active in vitro against the majority of isolates, even those that were resistant to fluconazole.
authors.affiliation: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid. mcuenca-estrella@isciii.es
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.forename: Dolores
authors.initials: D
authors.lastname: Rodriguez
authors.forename: Benito
authors.initials: B
authors.lastname: Almirante
authors.forename: Juliette
authors.initials: J
authors.lastname: Morgan
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Planes
authors.forename: Manel
authors.initials: M
authors.lastname: Almela
authors.forename: José
authors.initials: J
authors.lastname: Mensa
authors.forename: Ferran
authors.initials: F
authors.lastname: Sanchez
authors.forename: Josefina
authors.initials: J
authors.lastname: Ayats
authors.forename: Montserrat
authors.initials: M
authors.lastname: Gimenez
authors.forename: Margarita
authors.initials: M
authors.lastname: Salvado
authors.forename: David W
authors.initials: DW
authors.lastname: Warnock
authors.forename: Albert
authors.initials: A
authors.lastname: Pahissa
authors.forename: Juan L
authors.initials: JL
authors.lastname: Rodriguez-Tudela
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification* / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data / Q000639:trends; D011159:Population Surveillance / Q000379:methods; D013030:Spain / Q000453:epidemiology
chemical_list: D000935:Antifungal Agents