Interferon gene expression following HIV type 1 infection of monocyte-derived macrophages.
Macrophages represent one of the primary targets of HIV-1 infection. Changes in gene expression in primary human monocyte-derived macrophages following virus exposure were assessed using oligonucleotide arrays. Over a third of the 100 most modulated genes belonged to the interferon system. Upregulated interferon-stimulated genes included those essential for the innate immune response and also those involved in interferon and virus signal transduction from the cell surface. The promoter regions of a cluster of highly upregulated interferon-stimulated genes were analyzed for common regulatory elements. The nuclear factor in activated T cells (NFAT) and members of the interferon family of transcription factors appeared to be responsible for the upregulation of this set of interferon-stimulated genes following HIV-1 exposure.
Woelk
Christopher H
CH
Department of Pathology, University of California San Diego, La Jolla, California 92093-0679, USA. cwoelk@ucsd.edu
Ottones
Florence
F
Plotkin
Christine R
CR
Du
Pinyi
P
Royer
Christy D
CD
Rought
Steffney E
SE
Lozach
Jean
J
Sasik
Roman
R
Kornbluth
Richard S
RS
Richman
Douglas D
DD
Corbeil
Jacques
J
D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D007372:Interferons / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D009000:Monocytes / Q000276:immunology / Q000821:virology; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
D004268:DNA-Binding Proteins; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D011506:Proteins; D014157:Transcription Factors; D007372:Interferons