Truncated internalin A and asymptomatic Listeria monocytogenes carriage: in vivo investigation by allelic exchange.
Allelic exchange of the region coding for the C terminus of InlA between one epidemic (with an 80-kDa InlA) and one asymptomatic (with a 47-kDa InlA) carriage Listeria monocytogenes strain confirmed the need for this region for internalin entry in vitro. Interestingly, restoration of internalin A functionality did not result in full virulence in chicken embryo assays.
Olier
Maïwenn
M
Laboratoire de Microbiologie, UMR INRA 1232, Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France.
Garmyn
Dominique
D
Rousseaux
Sandrine
S
Lemaître
Jean-Paul
JP
Piveteau
Pascal
P
Guzzo
Jean
J
D000483:Alleles; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D018938:Caco-2 Cells; D002642:Chick Embryo; D006801:Humans; D008089:Listeria monocytogenes / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D014774:Virulence
D001426:Bacterial Proteins; C069761:internalin protein, Bacteria