title: Steroid administration before partial hepatectomy with temporary inflow occlusion does not influence cyclin D1 and Ki-67 related liver regeneration.
abstract: OBJECTIVE
  If temporary inflow occlusion is required during liver resection, the postoperative course might be complicated by ischaemia-reperfusion injury. Steroids protect against ischaemia-reperfusion injury; however, due to its anti-proliferative character concerns exist on its use on liver regeneration after resection. We investigated the effects of methylprednisolone on hepatocyte proliferation after partial hepatectomy with temporary inflow occlusion.
  
  
  METHODS
  Prior to surgery, one group of Wistar rats received methylprednisolone, while a second group served as non-treated controls. Ischaemia-reperfusion injury was indicated by AST, ALT, and GLDH at 6 h after surgery. Immunohistochemistry tools were used to determine the mitotic index and Ki-67 expression, while cyclin D1 expression characterized the proliferative activity on days 1, 4, 7, and 10.
  
  
  RESULTS
  The post-ischaemic liver enzyme release had significantly decreased in the methylprednisolone group, while expression of cyclin D1, percentage of Ki-67-positive cells, and mitotic cell index were comparable in both groups. Similar results were found for bilirubin and albumin and for weight of proliferating liver.
  
  
  CONCLUSIONS
  Although steroid administration significantly reduced ischaemia-reperfusion-associated tissue injury, it has no apparent effects on hepatic regeneration. Thus, steroids could be recommended if a temporary liver ischaemia is required during surgery, in order to reduce complications caused by severe ischaemia-related organ dysfunction.
authors.affiliation: Department of General Surgery, Visceral Surgery and Transplantation Surgery, Charité, Campus Virchow Klinikum, Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. matthias.glanemann@charite.de
authors.forename: Matthias
authors.initials: M
authors.lastname: Glanemann
authors.forename: Simone
authors.initials: S
authors.lastname: Münchow
authors.forename: Anja
authors.initials: A
authors.lastname: Schirmeier
authors.forename: Hussein
authors.initials: H
authors.lastname: Al-Abadi
authors.forename: Frank
authors.initials: F
authors.lastname: Lippek
authors.forename: Jan M
authors.initials: JM
authors.lastname: Langrehr
authors.forename: Manfred
authors.initials: M
authors.lastname: Dietel
authors.forename: Peter
authors.initials: P
authors.lastname: Neuhaus
authors.forename: Andreas K
authors.initials: AK
authors.lastname: Nussler
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D015153:Blotting, Western; D019938:Cyclin D1 / Q000032:analysis*; D003627:Data Interpretation, Statistical; D006498:Hepatectomy; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D019394:Ki-67 Antigen / Q000032:analysis*; D008115:Liver Regeneration; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage*; D011184:Postoperative Period; D011300:Preoperative Care; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D019394:Ki-67 Antigen; D019938:Cyclin D1; D008775:Methylprednisolone