[Segregation of sex chromosomes in the spermatozoa of 46, XY/47, XXY patients with oligozoospermia by dual fluorescence in-situ hybridization].
OBJECTIVE
To observe the segregation of sex chromosomes in the spermatozoa of a 46, XY/47, XXY patient with oligozoospermia.


METHODS
The number of X and Y chromosomes of the ejaculated spermatozoa from the patient with mosaic 46, XY/47, XXY was analysed by X/Y dual fluorescence in-situ hybridization (FISH).


RESULTS
Of the 100 spermatozoa analysed, 97 showed either one X chromosome-specific green signal or one Y-chromosome-specific red Y signal in each spermatozoon and only 3 showed no signal. The frequencies of X- and Y-bearing spermatozoa were 49% and 48% respectively. The ratio of X- to Y-bearing spermatozoa was about 1:1 as expected. There was no statistical difference between the chromosome XX and XY frequencies in each spermatozoon from the patient in comparison with those estimated in the control.


CONCLUSIONS
The spermatozoa of 46, XX/47, XXY mosaic patients have a normal gonosomal complement, which allows infertility treatment to be carried out by ICSI.
Cui
Yingxia
Y
Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. cuiyx55@yahoo.com
Ge
Yifeng
Y
Wang
Yunhua
Y
Zhou
Qin
Q
Wang
Yongmei
Y
Zhu
Peiyuan
P
Shang
Xuejun
X
Huang
Yufeng
Y
D000328:Adult; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007713:Klinefelter Syndrome / Q000235:genetics* / Q000628:therapy; D008297:Male; D009845:Oligospermia / Q000235:genetics* / Q000628:therapy; D020554:Sperm Injections, Intracytoplasmic