title: A novel lectin (Morniga M) from mulberry (Morus nigra) bark recognizes oligomannosyl residues in N-glycans.
abstract: Morniga M is a jacalin-related and mannose-specific lectin isolated from the bark of the mulberry (Morus nigra). In order to understand the function and application of this novel lectin, the binding property of Morniga M was studied in detail using an enzyme-linked lectinosorbent assay and lectin-glycan inhibition assay with extended glycan/ligand collection. From the results, it was found that the di-, tri-, and oligomannosyl structural units of N-glycans such as those of the bovine alpha1-acid glycoprotein (gp) and lactoferrin were the most active gps, but not the O-glycans or polysaccharides including mannan from yeast. The binding affinity of Morniga M for ligands can be ranked in decreasing order as follows: gps carrying multiple N-glycans with oligomannosyl residues >> N-glycopeptide with a single trimannosyl core > Tri-Man oligomer [Man alpha1-->6(Man alpha1-->3) Man], Penta-Man oligomer [Man alpha1-->6(Man alpha1-->3)Man alpha1-->6(Man alpha1-->3) Man] > or = Man alpha1-->2, 3 or 6 Man > Man > GlcNAc, Glc >> L-Fuc, Gal, GalNAc (inactive), demonstrating the unique specificity of this lectin that may not only assist in our understanding of cell surface carbohydrate ligand-lectin recognition, but also provide informative guidelines for the application of this structural probe in biotechnological and clinical regimens, especially in the detection and purification of N-linked glycans.
authors.affiliation: Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung University, Kweishan, Taoyuan, Taiwan. amwu@mail.cgu.edu.tw
authors.forename: Albert M
authors.initials: AM
authors.lastname: Wu
authors.forename: June H
authors.initials: JH
authors.lastname: Wu
authors.forename: Tanuja
authors.initials: T
authors.lastname: Singh
authors.forename: Kang-Chuang
authors.initials: KC
authors.lastname: Chu
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Peumans
authors.forename: Pierre
authors.initials: P
authors.lastname: Rougé
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008024:Ligands; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D031625:Morus; D009844:Oligosaccharides / Q000737:chemistry; D009961:Orosomucoid / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D037102:Lectins; D008024:Ligands; D009844:Oligosaccharides; D009961:Orosomucoid; D037121:Plant Lectins; D010940:Plant Proteins; D011134:Polysaccharides; C500656:morniga M; D008358:Mannose