title: [Study on DNA damage and expression of RAS gene in L-02 cells exposed to chlorinated drinking water extracts].
abstract: OBJECTIVE
  To study the DNA damage and expression of RAS gene in human fetal hepatocytes (L-02) induced by chlorinated drinking water extracts, and to explore the possible molecular mechanism of its genotoxicity .
  
  
  METHODS
  L-02 cells were exposed to chlorinated drinking water extracts at the concentration of 0.167, 1.67, 16.7 and 167 ml/ml culture medium. Single cell gel electrophoresis (SCGE) and in situ hybridization (ISH) assay were used to detect DNA damage and the expression of RAS gene in L-02 cells. DMSO (10 ml/L) and B(a)P (200 micromol/L) were used as solvent control and positive control respectively.
  
  
  RESULTS
  Chlorinated drinking water extracts led to a significant increase of DNA migration in the L-02 cells at the concentrations of 16.7 and 167 ml/ml culture medium in comparison with solvent control. Significantly higher expressions of RAS gene was found in the L-02 cells exposed to all concentrations of chlorinated drinking water extracts than in that of solvent control group. There was a significant correlation (r = 0.99) between RAS gene expression and DNA migration when the concentration of chlorinated drinking water extracts was between 0.167 and 16.7 ml/ml.
  
  
  CONCLUSIONS
  Chlorinated drinking water extracts could induce obvious DNA damage and RAS gene expression in L-02 cells, and RAS gene expression might be affected by DNA damage.
authors.affiliation: Department of Occupational and Environmental Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Yaling
authors.initials: Y
authors.lastname: Zou
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Wenqing
authors.initials: W
authors.lastname: Lu
mesh_terms: D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D011905:Genes, ras; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D017403:In Situ Hybridization; D014881:Water Supply
chemical_list: D006843:Hydrocarbons, Chlorinated