Immunohistochemical localization of androgen receptor and aromatase in the ovary of the pregnant pig.
The distribution of androgen receptor (AR) and cytochrome P450 aromatase was investigated in paraffin sections of pregnant pig ovary. Ovarian follicles and corpora lutea were isolated from ovaries obtained on Days 10, 18, 32, 71 and 90 post coitum (p.c.). Androgen receptor was localized in the nuclei of granulosa cells of follicles of various sizes. In addition, some follicles demonstrated staining in the nuclei of theca interna cells. Stroma cells also exhibited a positive immunostaining. At early and mid pregnancy (up to Day 71) AR was expressed in the nuclei of luteal cells. Corpora lutea of Day 71 showed mainly cytoplasmic staining while on Day 90 almost all luteal cells showed staining exclusively in the cytoplasm. Immuno-staining for the presence of cytochrome P450 aromatase was very faint in all investigated ovarian structures. The results could suggest that the process of androgen aromatization plays a negligible role in the ovary of the pregnant pig.
Duda
Małgorzata
M
Laboratory of Endocrinology and Tissue Culture, Institute of Zoology, Jagiellonian University, Kraków, Poland.
Burek
Małgorzata
M
Galas
Jerzy
J
Kozioł
Katarzyna
K
Koziorowski
Marek
M
Słomczyńska
Maria
M
D000818:Animals; D001141:Aromatase / Q000032:analysis*; D002467:Cell Nucleus / Q000201:enzymology; D003338:Corpus Luteum / Q000737:chemistry / Q000648:ultrastructure; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D005865:Gestational Age; D006107:Granulosa Cells / Q000737:chemistry / Q000648:ultrastructure; D007150:Immunohistochemistry; D010053:Ovary / Q000737:chemistry* / Q000201:enzymology; D011247:Pregnancy; D011944:Receptors, Androgen / Q000032:analysis*; D017154:Stromal Cells / Q000737:chemistry; D013552:Swine; D013799:Theca Cells / Q000737:chemistry / Q000648:ultrastructure; D014018:Tissue Distribution
D011944:Receptors, Androgen; D001141:Aromatase