title: Effects of U-37883A on intracellular Ca2+ -activated large-conductance K+ channels in pig proximal urethral myocytes.
abstract: Kinetic studies of U-37883A (4-morpholinecarboximidine-N-1-adamantyl-N'-cyclohexyl-hydrochloride), a vascular ATP-sensitive K+ channel (KATP channel) blocker, were performed on pig urethral myocytes to investigate inhibitory effects on large-conductance intracellular Ca2+ -sensitive K+ channels (i.e., BKCa channels; 225 pS K+ channels) by use of single-channel recordings (outside-out and inside-out configuration). BKCa channels in pig urethral smooth muscles showed extracellular iberiotoxin (300 nM) sensitivity and voltage dependency. The alpha subunit of BKCa channel proteins was detected in the membrane fraction by use of Western blot technique. Application of U-37883A (> or =10 microM) reduced the activity of BKCa channels in a concentration-dependent manner, not only by decreasing mean openlife time but also by prolonging the mean closed time. These results shows that U-37883A affects channels other than the vascular KATP channel, and demonstrates how it inhibits the activities of BKCa channels in urethral smooth muscles.
authors.affiliation: Department of Pharmacology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi Ward, Fukuoka 812-8582, Japan. noritera@linne.med.kyushu-u.ac.jp
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Teramoto
authors.forename: Manami
authors.initials: M
authors.lastname: Aishima
authors.forename: Hai-Lei
authors.initials: HL
authors.lastname: Zhu
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Tomoda
authors.forename: Takakazu
authors.initials: T
authors.lastname: Yunoki
authors.forename: Fumi
authors.initials: F
authors.lastname: Takahashi-Yanaga
authors.forename: Alison F
authors.initials: AF
authors.lastname: Brading
authors.forename: Yushi
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D009025:Morpholines / Q000494:pharmacology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D024681:Potassium Channels, Calcium-Activated / Q000502:physiology*; D013552:Swine; D013997:Time Factors; D014521:Urethra / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D009025:Morpholines; D024681:Potassium Channels, Calcium-Activated; C082658:U 37883A; D000218:Adamantane; D002118:Calcium