title: Ion transport in rabbit caecum at 12 and 36 months of age.
abstract: The aim of this study was to investigate the effect of aging on sodium and chloride ions transport in the isolated rabbit caecum. The experiment consisted in measuring PD- transepithelial electrical potential difference mV SM-mechanical stimulation and mechano-sensitivity (dPD) in collected from 12- and 36-month-old rabbits. A modified Ussing system was used for the experiment. Under the incubation in Ringer solution the values of PD and dPD of young rabbit caecum were, respectively, -3.0 +/-0.4 mV and -1.2 +/-0.3 mV. In 36-month rabbits, on the other hand, the values of these parameters were lower, PD by about 90% whereas dPD by about 83%. Incubation of young rabbits caecum with the presence of amiloride resulted in a reduction of PD by about 17 % and dPD by about 50%, while it did not influence the electrophysiological parameters of caecum in 36-month rabbits. Incubation with bumetanide on young rabbits caecum resulted in PD dropped by about 70% and dPD by about 83%. In the same incubation conditions as those applied for incubation of 36-month rabbits caecum, the level of PD decreased by about 67% while dPD by about 50%. Incubation of young rabbits caecum at the presence of both transport inhibitors resulted in PD decreased by about 87% and dPD by about 83%, whereas that applied for the old rabbits' caecum reduced PD by about 90%, without influencing dPD. Presented data demonstrate that the process of organism ageing inhibits sodium ion transport as well as reduces caecum epithelium sensitivity to mechanical stimuli. Probably, it is a consequence of a reduced with age release of neurotransmitter from sensory terminals; these neurotransmitters modify transepithelial ion transport.
authors.affiliation: Department of Biology and Medical Parasitology, Pomeranian Medical University, Szczecin, Poland. kodan@sci.pam.szczecin.pl
authors.forename: D
authors.initials: D
authors.lastname: Kosik-Bogacka
authors.forename: T
authors.initials: T
authors.lastname: Tyrakowski
mesh_terms: D000367:Age Factors; D000375:Aging / Q000502:physiology; D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D002432:Cecum / Q000378:metabolism* / Q000502:physiology; D005260:Female; D066298:In Vitro Techniques; D017136:Ion Transport / Q000502:physiology*; D008297:Male; D011817:Rabbits; D012964:Sodium / Q000378:metabolism / Q000502:physiology
chemical_list: D012964:Sodium