Poliovirus replicons encoding the B subunit of Helicobacter pylori urease protect mice against H. pylori infection.
We developed a novel vaccine for Helicobacter pylori based on a poliovirus vector in which capsid genes were replaced with the gene for the B subunit of H. pylori urease (UreB). Mice were vaccinated with UreB or control (L1) replicon and challenged with H. pylori. Twenty percent of mice vaccinated prophylactically with UreB, but 80% vaccinated with L1, and then challenged with H. pylori became infected (P = 0.003). Seventy-three percent of mice with established H. pylori infection vaccinated therapeutically with UreB replicon cleared their infection compared to 33% vaccinated with L1 (P = 0.067). In therapeutically vaccinated mice with residual infection, UreB-vaccinated animals had fewer H. pylori than L1-vaccinated mice (P < 0.05). Anti-urease antibody titres in prophylactically, but not therapeutically, vaccinated mice were markedly higher in animals that received UreB versus L1 replicon (P = 0.01). Vaccination with poliovirus vector containing the gene for the B subunit of H. pylori urease provides significant prophylactic and strong therapeutic protection against H. pylori in mice.
Smythies
Lesley E
LE
Department of Medicine (Gastroenterology), University of Alabama at Birmingham, ZRB 633, 703 19th Street South, Birmingham, AL 35294, USA.
Novak
Miroslav J
MJ
Waites
Ken B
KB
Lindsey
J Russell
JR
Morrow
Casey D
CD
Smith
Phillip D
PD
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D006367:HeLa Cells; D016481:Helicobacter Infections / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology; D012093:Replicon / Q000235:genetics* / Q000276:immunology; D014510:Urease / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D001428:Bacterial Vaccines; D022223:Vaccines, Subunit; D014510:Urease