p53, p63 and p73 expression in squamous cell carcinomas of the head and neck and their response to cisplatin exposure.
p63 and p73 share significant structural and functional homologies with the tumour suppressor p53. Unlike the p53 gene, both encode for several isoforms which vary in their NH2 and COOH termini with variable and, in part, opposed biological functions. The objective of the present study was to analyse the expression profiles of p53 family members in squamous cell carcinomas of the head and neck (HNSCC) and their alterations caused by exposure to the clinically active drug cisplatin. Using multiplex RT-PCR combined with the Southern technique, we determined transcription of p53 family members in 10 established HNSCC cell lines. In the majority of HNSCC, p53 and different p63/p73 isoforms were expressed with cell-line-specific patterns for composition and intensity of transcript expression. Exposure to cisplatin caused multiple alterations in the p63 and p73 profiles suggesting a complex regulation which may influence the sensitivity to chemotherapy.
Gwosdz
Christian
C
Department of Otorhinolaryngology/Head and Neck Surgery, Heinrich Heine University, Düsseldorf, Germany.
Balz
Vera
V
Scheckenbach
Kathrin
K
Bier
Henning
H
D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000401:mortality; D002945:Cisplatin / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000401:mortality; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016019:Survival Analysis; D014407:Tumor Cells, Cultured; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D002945:Cisplatin