[Determination of concentration and encapsulation efficiency of silybin liposomes].
OBJECTIVE
To determine the encapsulation efficiency of silybin liposomes.


METHODS
After Silybin liposomes solution was separated through sphadex G-100 column, eluent collected was determined by the first derivative UV spectrometry.


RESULTS
The calibration curve of Silybin was linear in the range of 0.5-30.0 mg x L(-1) (r = 0.9998). The average recovery was 101.7%. The relative standard deviation was 1.8%. The results showed that the encapsulation efficiency ranged from 65.1%-83.0% when the drug/phospholipid ratio varied from 0.02-0.06. The concentration of SLB in liposomes prepared by optimum technique was 760.4 mg x L(-1) (n = 4).


CONCLUSIONS
The method is accurate, simple and suitable for the quality control of Silybin liposomes.
Yu
Jiang-nan
JN
School of Pharmacy, Jiangsu University, Zhenjiang 212001, Jiangsu, China.
Xu
Xi-ming
XM
Zhu
Yuan
Y
Gao
Hao
H
D016503:Drug Delivery Systems; D008081:Liposomes / Q000737:chemistry; D020944:Silybum marianum / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D000077385:Silybin; D012838:Silymarin / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods
D008081:Liposomes; D012838:Silymarin; D000077385:Silybin