TaqMan-based quantification of invasive cells in the chick embryo metastasis assay.
The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan technology to quantify the invaded tumor cells in the chorioallantoic membrane of fertilized eggs. This method is based on specific detection of human ALU sequences. Moreover, it provides high sensitivity over a wide linearity range.
van der Horst
Edward Htun
EH
U3Pharma, Martinsried, Germany.
Leupold
Jörg H
JH
Schubbert
Rainer
R
Ullrich
Axel
A
Allgayer
Heike
H
D020087:Alu Elements / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D002642:Chick Embryo; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology* / Q000556:secondary*; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000473:pathology* / Q000556:secondary*; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm