title: [Enzyme modification by natural chemical chaperons of microorganisms].
abstract: We demonstrated for the first time that alkylhydroxybenzenes (the d1 microbial autoregulatory factors involved in stress responses of cells) are capable of stabilizing enzymes in aqueous media and increasing their catalytic activity. The stabilizing effect of a chemical analogue of alkylhydroxybenzenes, C7-AHB, was established in in vitro studies with enzymes of microbial origin: a protease produced by Bacillus licheniformis, cellulase produced by Trichoderma viride, and alpha-amylase produced by Bacillus subtilis. This effect manifested itself in considerable extension of the temperature and pH ranges of the enzymatic activity. The modulation of the catalytic activities of the stabilized enzymes depended on the C7-AHB concentration and on the time of preincubation of the complexes obtained. We demonstrated that not only enzymes but also their polymeric substrates formed complexes with C7-AHB, and this also significantly influenced the efficiency of hydrolytic reactions. We also conducted comparative studies on the efficiency of hydrolytic reactions in systems in which the structure of enzymes and/or substrates was modified with C7-AHB.
authors.forename: E I
authors.initials: EI
authors.lastname: Martirosova
authors.forename: T A
authors.initials: TA
authors.lastname: Karpekina
authors.forename: G I
authors.initials: GI
authors.lastname: El'-Registan
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D044602:Cellulases / Q000378:metabolism; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D018832:Molecular Chaperones / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000378:metabolism; D019800:Phenol / Q000494:pharmacology*; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology*; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D018832:Molecular Chaperones; D019800:Phenol; D044602:Cellulases; D000516:alpha-Amylases; D010447:Peptide Hydrolases