[Experimental study on anticancer effect of curcumin on Raji cells in vitro].
OBJECTIVE
To study the anticancer effect and mechanism of curcumin on Raji cells in vitro and compared the cytotoxicities of curcumin on Raji cells and normal human peripheral blood mononuclear cell (PBMC).


METHODS
The effects of curcumin on proliferation of Raji cells and human PBMC were tested by MTT assay, its effects on apoptosis of them were determined by Annexin-V/PI double-labeled cytometry and TUNEL, and its effects on DNA distribution in Raji cells was studied by PI single labeled cytometry.


RESULTS
Curcumin showed marked inhibition on proliferation of Raji cell, could induce Raji cell apoptosis in time- and dose-dependent manner. After curcumin treatment, the cell cycle of Raji cells was blocked in G0/G1 and G2/M phase and those in the S phase decreased proportionally. But curcumin showed no significant effect on inhibiting proliferation or inducing apoptosis on human PBMC.


CONCLUSIONS
Curcumin could regulate the cell cycle of Raji cells and induce its apoptosis, so as to inhibit its proliferation, but with no significant cytotoxicity on human PBMC. It selectively affects the tumor cell.
Sun
Chun-yan
CY
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan (430022). ayan0618@163.com
Liu
Xin-yue
XY
Chen
Yan
Y
Liu
Fang
F
Wang
Yan
Y
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D016393:Lymphoma, B-Cell / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D003474:Curcumin