title: The adhesion force of Notch with Delta and the rate of Notch signaling.
abstract: Notch signaling is repeatedly used during animal development to specify cell fates. Using atomic force microscopy on live cells, chemical inhibitors, and conventional analyses, we show that the rate of Notch signaling is linked to the adhesion force between cells expressing Notch receptors and Delta ligand. Both the Notch extracellular and intracellular domains are required for the high adhesion force with Delta. This high adhesion force is lost within minutes, primarily due to the action of Presenilin on Notch. Reduced turnover or Delta pulling accelerate this loss. These data suggest that strong adhesion between Notch and Delta might serve as a booster for initiating Notch signaling at a high rate.
authors.affiliation: Department of Microbiology and Molecular Genetics, The University of Vermont, VT 05405, USA.
authors.forename: Francois
authors.initials: F
authors.lastname: Ahimou
authors.forename: Lee-Peng
authors.initials: LP
authors.lastname: Mok
authors.forename: Boris
authors.initials: B
authors.lastname: Bardot
authors.forename: Cedric
authors.initials: C
authors.lastname: Wesley
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D004330:Drosophila; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D018625:Microscopy, Atomic Force / Q000379:methods; D051880:Receptors, Notch; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors
chemical_list: D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; C064045:delta protein