Polyphenol oxidase expression in potato (Solanum tuberosum) tubers inhibited to sprouting by treatment with iodine atmosphere.
Iodine-saturated atmosphere was found to inhibit the sprouting of potato (Solanum tuberosum L.) tubers. The iodine concentration in tuber tissues increased as a function of exposure length, and the onset of inhibition of sprouting was found to depend on tubers genotype. During the time-course of the treatment, the transcription of polyphenol oxidases (EC 1.10.3.1 and EC 1.14.18.1) was undetectable in tuber peel, whereas in bud tissues featured an increase, followed by a decrease occurring simultaneously with the suppression of sprouting. The treatment of tubers with iodine strongly affected the expression of polyphenol oxidases at the transcriptional level. Polyphenol oxidase activity in buds poorly reflected the corresponding level of transcription; similarly, little differences were found among the enzyme isoforms expressed in buds as a function of length of exposure to iodine. These findings suggest that the induction of polyphenol oxidases mRNAs transcription could probe the inhibition of sprouting by iodine.
Eolini
Francesco
F
Department of Industrial Chemistry, University of Bologna, Viale Risorgimento 4, I-40136 Bologna, Italy.
Hochkoeppler
Alejandro
A
Credi
Andrea
A
Rodríguez
Antonio Gonzàlez Vara Y
AG
Poggi
Valeria
V
D015152:Blotting, Northern; D004156:Catechol Oxidase / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005511:Food Handling / Q000592:standards; D007455:Iodine / Q000494:pharmacology*; D010940:Plant Proteins / Q000096:biosynthesis*; D035281:Plant Tubers / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D018749:RNA, Plant / Q000096:biosynthesis; D011198:Solanum tuberosum / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D014158:Transcription, Genetic / Q000187:drug effects
D010940:Plant Proteins; D018749:RNA, Plant; D007455:Iodine; D004156:Catechol Oxidase