Development and evaluation of a protein microarray chip for diagnosis of hepatitis C virus.
A protein chip diagnostic kit was developed for the diagnosis of hepatitis C virus (HCV) based on the protein chip technique and the immuno-concentration method. This kit was designed for low-density protein chips and also for the availability of multiple sample screening. Applicability of the chip was evaluated using 96 blood specimens and the results were compared to results of an anti-HCV enzyme immunoassay (EIA) test. With further development, the technology associated with the development of this chip could be applied to the simultaneous detection of multiple protein-protein, protein-ligand interactions.
Yuk
Chul-Soo
CS
Laboratory of Biochemistry, College of Life and Environmental Sciences, Korea University, Seoul 136-701, Korea.
Lee
Hyung Ku
HK
Kim
Hee Tae
HT
Choi
Yong Keel
YK
Lee
Byung Cheon
BC
Chun
Bok-Hwan
BH
Chung
Namhyun
N
D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006526:Hepatitis C / Q000097:blood* / Q000175:diagnosis*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018937:Hepatitis C Antibodies