Inhibition of the benzo[a]pyrene-induced toxicity by allyl sulfides in human epidermal keratinocytes.
Diallyl sulfide (DAS) and diallyl disulfide (DADS) at 25 microg ml(-1)decreased the benzo[a]pyrene (B[a]P)-induced colony growth inhibition of human epidermal keratinocytes. DAS and DADS decreased B[a]P-DNA and B[a]P-protein adducts by 65% and 49-55%, respectively. The B[a]P-induced ethoxyresorufin O-deethylase activity, a marker enzyme for cytochrome P450 1, was decreased from 3 to 1.7-1.9 nmol min(-1) mg(-1) microsomal protein by DAS and DADS treatments. The activity of glutathione S-transferase, a detoxifying enzyme for B[a]P, but was decreased by DADS, but was unaffected by DAS.
Chun
Hyang Sook
HS
Korea Food Research Institute, Sungnam, 463-746, Korea. hschun@kfri.re.kr
Kim
Hyun Jung
HJ
Kim
Yangha
Y
Chang
Hyun Joo
HJ
D000498:Allyl Compounds / Q000633:toxicity*; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004220:Disulfides / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects*; D013440:Sulfides / Q000633:toxicity*
D000498:Allyl Compounds; D004220:Disulfides; D013440:Sulfides; D001564:Benzo(a)pyrene; C028009:diallyl disulfide; C038491:allyl sulfide