Concomitant translocation of Puralpha with its binding proteins (PurBPs) from nuclei to cytoplasm during neuronal development.
Two Puralpha-binding proteins (PurBPs) were found in nuclear extract from mouse brain during P4-P10 by the overlay assay. At P14, they were decreased significantly in nuclear extract and increased in the S3 fraction, indicating their dynamic translocation during development. Western blot analysis also demonstrated concomitant translocation of Puralpha with the PurBPs during P7-P14, when neuronal circuit proceeds. Immunocytochemical study with cultured hippocampal neurons from rat E18 confirmed that nuclear Puralpha was translocated to cytoplasm after plating for 7-14 days. These results suggest that spatiotemporal translocation of Puralpha with the PurBPs from nuclei to cytoplasm has a crucial role in neuronal development.
Zeng
Ling-Hui
LH
Department of Pharmacology, Osaka University Medical School, 2-2 Yamadaoka Suita, Osaka 565-0871, Japan.
Okamura
Ko
K
Tanaka
Hidekazu
H
Miki
Naomasa
N
Kuo
Che-Hui
CH
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004622:Embryo, Mammalian; D006624:Hippocampus / Q000166:cytology* / Q000254:growth & development; D007150:Immunohistochemistry / Q000379:methods; D007211:Indoles / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D007211:Indoles; D009419:Nerve Tissue Proteins; C121485:Pura protein, mouse; D014157:Transcription Factors; C007293:DAPI