[Effect of transforming growth factor beta1 plasmid on frosted allogenic nerve transplantation].
OBJECTIVE
To study the effect of transforming growth factor beta1 (TGF-beta1) plasmid on poly frosted-defrosted allogenic nerve transplantation.


METHODS
Forty Wistar rats were randomly divided into two groups equally. A 2.0 cm sciatic nerve segment, 5 mm away from infrapiriformis muscle space, was removed and the defect was repaired with poly frosted-defrosted allogenic nerve. The TGF-beta1 plasmids were injected into the nerve anastomosis and adjacent muscles in the experimental group, normal saline in the control group. The nerve specimens were sectioned for staining in the 6th and 12th weeks. Axonal count and statistical analyses were done.


RESULTS
The grafted and distal nerve segments showed regenerated fibers in both groups. In the experimental group, less edema and more nerve fibers were observed in the 6th week. The grafted nerve segment was filled with regeneration axons, the myelinated nerve fibers arranged regularly, and the axons and the myelin sheaths developed well in the 12th week. There was significant difference in the number of regenerating axons between the experimental group 98.6 +/- 4.8/microm2 and control group 75.8 +/- 5.1/microm2 (P < 0.01).


CONCLUSIONS
Multiple frost-defrost of allogenic nerve can reduce its antigenicity and increase its usefulness in repairing nerve defects. Local use of TGF-beta1 plasmid can enhance immunosuppression to reduce immuno rejection.
Weng
Yuxiong
Y
Department of Hand Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan Hubei, 430022, PR China.
Chen
Zhenbing
Z
Huang
Qishun
Q
D000818:Animals; D015925:Cryopreservation; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D008297:Male; D009416:Nerve Regeneration; D010957:Plasmids / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries* / Q000601:surgery / Q000637:transplantation*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D014184:Transplantation, Homologous
C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1