title: The complete genome sequence of a new necrovirus isolated from Olea europaea L.
abstract: The complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive latent virus 1 (OLV-1) and the coat protein reveals highest sequence identity with that of TNV-D. Based on the deduced genome organization and phylogenetic analysis of predicted functional translation products with that of other necroviruses, the GP isolate genome appears to represent an example of a new virus arisen by gene exchange and is proposed to be a new necrovirus, provisionally named Olive mild mosaic virus.
authors.affiliation: Instituto de Ciências Agrárias Mediterrânicas, Universidade de Evora, Evora, Portugal.
authors.forename: J M S
authors.initials: JM
authors.lastname: Cardoso
authors.forename: M R
authors.initials: MR
authors.lastname: Félix
authors.forename: M I E
authors.initials: MI
authors.lastname: Clara
authors.forename: S
authors.initials: S
authors.lastname: Oliveira
mesh_terms: D019659:Asteraceae / Q000821:virology*; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D019183:Tombusviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers