title: [Advanced oxidation protein products induce reactive oxygen species production in endothelial cells].
abstract: OBJECTIVE
  To investigate the effect of advanced oxidation protein products (AOPP) on production of reactive oxygen species (ROS) in endothelial cells.
  
  
  METHODS
  Human umbilical vein endothelial cell line ECV304 were stimulated with AOPP-modified bovine serum albumin (AOPP-BSA) prepared by oxidation of BSA with HOCl in vitro. ROS production in the cells was evaluated by kinetic measurement of dichlorofluoroscein (DCF) fluorescence produced by oxidation of an oxidant-sensitive dye 2,7-dichlorefluorescin (DCFH) using VICTOR 1420 multilabel counter.
  
  
  RESULTS
  AOPP-BSA could induce ROS production in ECV304 cells time- and dose-dependently, and the quantity of ROS increased with the oxidation degree of BSA. Pretreatment of cells with a small-molecular-mass glutathione peroxidase mimic ebselen or NADPH oxidase inhibitor apocynin inhibited ROS production by 65% and 29% respectively.
  
  
  CONCLUSIONS
  AOPP-BSA induces ROS production in endothelial cells, partially mediated by NADPH oxidase activation.
authors.affiliation: Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Shang-xi
authors.initials: SX
authors.lastname: Liu
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Tian
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D019255:NADPH Oxidases / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011506:Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
chemical_list: D011506:Proteins; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D019255:NADPH Oxidases