title: A new method of perfusion fixation for the rabbit femur.
abstract: We report a new method of perfusion fixation for the proximal one-third of the femur of the Japanese white rabbit. Fluids to flush the blood and fix the marrow were injected into the abdominal aorta and drained from the stump of the femur. The oozing of the fluids from the stumps guaranteed complete flushing and fixation. The new method facilitated fixation and decreased the volume of necessary fluids. Scanning electron microscopy (SEM) images of bone marrow fixed using the new method and using the conventional method did not differ. Large fat globules were not observed in the SEM specimens produced using either the new or the conventional method.
authors.affiliation: Department of Orthopedics, Kanazawa Medical University, Uchinada 1-1, Ishikawa 920-0293, Japan.
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Fukui
authors.forename: Rieko
authors.initials: R
authors.lastname: Kominami
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kaneuji
authors.forename: Tadami
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Harumichi
authors.initials: H
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D005260:Female; D005269:Femur / Q000098:blood supply / Q000648:ultrastructure*; D007281:Ink; D008855:Microscopy, Electron, Scanning; D010477:Perfusion / Q000379:methods*; D011817:Rabbits; D016707:Tissue Fixation / Q000379:methods*