A mini exon in the sucrose:sucrose 1-fructosyltransferase gene of wheat.
We previously reported the cloning of a wheat sucrose:sucrose 1-fructosyltransferase (1-SST) cDNA, designated wft2. Wft2 proteins have fructosyltransferase enzyme activity and initiate fructan synthesis (Biosci. Biotechnol. Biochem. 66 (2002) 2297). In the current study, we cloned a genomic DNA fragment carrying the full-length 1-SST gene from winter wheat (Triticum aestivum). The genomic 1-SST gene is 3326 bp in length and contains four exons and three introns. Exon 2 has only 9 bp. This sequence encodes a part of a beta-fructosidase motif (NDPNG), a highly conserved motif found in plant invertases. This is the first report of a mini exon, one of the smallest exons known in plants, being found in a genomic 1-SST gene.
Yoshida
Midori
M
National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo 062-8555, Japan. midori@affrc.go.jp
Lin
Dingbo
D
Kawakami
Akira
A
D016384:Consensus Sequence; D018076:DNA, Complementary / Q000032:analysis; D018744:DNA, Plant / Q000235:genetics; D005091:Exons / Q000235:genetics*; D006602:Hexosyltransferases / Q000032:analysis / Q000235:genetics*; D007438:Introns / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins
D018076:DNA, Complementary; D018744:DNA, Plant; D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins