Determination of oligoelements in parenteral formulations by planar chromatography and spectrophotometry.
The authors have studied the chromatographic behavior of parenteral preparations for pediatric use containing inorganic cations. After separation and identification by thin-layer chromatography, Mn(2+), Zn(2+), and Cu(2+) were analyzed by a method based on reaction with an appropriate reagent and extraction with an organic solvent which yielded elution and preconcentration, resulting in an appropriate solution for colorimetric quantitation. Cr(3+) cation was determined by atomic absorption spectrophotometry after appropriate chromatographic separation, using microcrystalline cellulose (adsorbent) and an acetone:water:hydrochloric acid mixture (80:5:8) as the mobile phase.
Mancini
M A
MA
Department of Pharmaceuticals, Faculty of Pharmaceutical Sciences, UNESP, 14801-902, Araraquara, São Paulo, Brazil.
Zuanon Netto
J
J
D002626:Chemistry, Pharmaceutical; D002675:Child, Preschool; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003300:Copper / Q000032:analysis*; D006801:Humans; D007223:Infant; D008345:Manganese / Q000032:analysis*; D010288:Parenteral Nutrition; D010372:Pediatrics; D015032:Zinc / Q000032:analysis*
D008345:Manganese; D003300:Copper; D015032:Zinc