title: [Immunoscreening of newborn larvae cDNA library of Trichinella spiralis].
abstract: OBJECTIVE
  To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis.
  
  
  METHODS
  Newborn larvae (NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed.
  
  
  RESULTS
  158 positive clones were obtained from 4.5 x 10(5) recombinant clones by immunoscreening. The sequence analysis of positive clones showed that there are 36 novel cDNAs, 5 reported cDNAs and 12 cDNAs without the ORF showing high similarity to the mitochondrial DNA of Trichinella spiralis.
  
  
  CONCLUSIONS
  Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.
authors.affiliation: Faculty of Military Veterinary, Quartermaster University of PLA, Changchun 130062, China.
authors.forename: Xiu-ping
authors.initials: XP
authors.lastname: Wu
authors.forename: Bao-quan
authors.initials: BQ
authors.lastname: Fu
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Liu
authors.forename: Ya-lan
authors.initials: YL
authors.lastname: Zhang
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Yuan
authors.forename: Lian-rui
authors.initials: LR
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Pascal
authors.initials: P
authors.lastname: Boireau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D006801:Humans; D007814:Larva / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013552:Swine; D017160:Trichinella spiralis / Q000235:genetics* / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D018076:DNA, Complementary