Expression of recombinant endostatin in Agrobacterium-inoculated leaf disks of Nicotiana tabacum var. Xanthi.
Recombinant endostatin was transiently expressed in Agrobacterium-inoculated leaf disks of Nicotiana tabacum var. Xanthi with a molecular size of 23 kDa. Expression of endostatin from a replicating vector based on tomato golden mosaic virus (TGMV) was 170% higher at the transcript level and double higher at the protein level than from a control vector of a non-replicating construct. Purified recombinant endostatin from tobacco leaf-disks has an anti-proliferative effect on bovine endothelial cells.
Hong
Seong Hyun
SH
Department of Genetic Engineering and Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.
Kim
Kyung Il
KI
Chung
Ho Yong
HY
Kim
You Jung
YJ
Sunter
Garry
G
Bisaro
David M
DM
Chung
In Sik
IS
D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D043169:Endostatins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D012231:Rhizobium / Q000235:genetics* / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods
D043169:Endostatins; D011994:Recombinant Proteins