title: Genotype distribution of Candida albicans isolates by 25S intron analysis with regard to invasiveness.
abstract: The aim of this study was to genotype Candida albicans strains isolated from patients with invasive and non-invasive deep-seated infections. For this purpose, 301 C. albicans isolates (81 invasive and 220 non-invasive) were genotyped by using specific PCR primers designed to span the transposable group I intron of the 25S rDNA gene. Fifty-three of the 81 invasive isolates were genotype A (65.4%), eight were genotype B (9.9%) and 20 were genotype C (24.7%), while 98 of the 220 non-invasive isolates were genotype A (44.6%), 46 were genotype B (20.9%) and 76 were genotype C (34.5%). Genotype A was more prevalent among invasive isolates and genotypes B and C were more prevalent among non-invasive isolates (P = 0.0046). Genotypes D and E which represent C. dubliniensis were not found. These results indicate that there may be a relationship between C. albicans genotypes and invasiveness; genotype A being more invasive than others. The presence or absence of the transposable group I intron in the 25S rDNA gene may be important in determining the invasiveness of C. albicans.
authors.affiliation: Division of Pediatric Molecular Pathology and Genetics, Faculty of Medicine, Ankara University, Ankara, Turkey. ckarahan@medicine.ankara.edu.tr
authors.forename: Z C
authors.initials: ZC
authors.lastname: Karahan
authors.forename: H
authors.initials: H
authors.lastname: Güriz
authors.forename: H
authors.initials: H
authors.lastname: Ağirbaşli
authors.forename: N
authors.initials: N
authors.lastname: Balaban
authors.forename: J S
authors.initials: JS
authors.lastname: Göçmen
authors.forename: D
authors.initials: D
authors.lastname: Aysev
authors.forename: N
authors.initials: N
authors.lastname: Akar
mesh_terms: D000293:Adolescent; D000328:Adult; D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007438:Introns; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D014421:Turkey; D014774:Virulence / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S