title: TaqMan-based quantification of invasive cells in the chick embryo metastasis assay.
abstract: The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan technology to quantify the invaded tumor cells in the chorioallantoic membrane of fertilized eggs. This method is based on specific detection of human ALU sequences. Moreover, it provides high sensitivity over a wide linearity range.
authors.affiliation: U3Pharma, Martinsried, Germany.
authors.forename: Edward Htun
authors.initials: EH
authors.lastname: van der Horst
authors.forename: Jörg H
authors.initials: JH
authors.lastname: Leupold
authors.forename: Rainer
authors.initials: R
authors.lastname: Schubbert
authors.forename: Axel
authors.initials: A
authors.lastname: Ullrich
authors.forename: Heike
authors.initials: H
authors.lastname: Allgayer
mesh_terms: D020087:Alu Elements / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D002642:Chick Embryo; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology* / Q000556:secondary*; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000473:pathology* / Q000556:secondary*; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm