title: Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds.
abstract: The major challenge of tissue engineering is directing the cells to establish the physiological structure and function of the tissue being replaced across different hierarchical scales. To engineer myocardium, biophysical regulation of the cells needs to recapitulate multiple signals present in the native heart. We hypothesized that excitation-contraction coupling, critical for the development and function of a normal heart, determines the development and function of engineered myocardium. To induce synchronous contractions of cultured cardiac constructs, we applied electrical signals designed to mimic those in the native heart. Over only 8 days in vitro, electrical field stimulation induced cell alignment and coupling, increased the amplitude of synchronous construct contractions by a factor of 7, and resulted in a remarkable level of ultrastructural organization. Development of conductive and contractile properties of cardiac constructs was concurrent, with strong dependence on the initiation and duration of electrical stimulation.
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E25-342, Cambridge, MA 02139, USA.
authors.forename: Milica
authors.initials: M
authors.lastname: Radisic
authors.forename: Hyoungshin
authors.initials: H
authors.lastname: Park
authors.forename: Helen
authors.initials: H
authors.lastname: Shing
authors.forename: Thomas
authors.initials: T
authors.lastname: Consi
authors.forename: Frederick J
authors.initials: FJ
authors.lastname: Schoen
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Freed
authors.forename: Gordana
authors.initials: G
authors.lastname: Vunjak-Novakovic
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology; D004560:Electricity; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology; D006321:Heart / Q000502:physiology*; D006352:Heart Ventricles / Q000166:cytology / Q000473:pathology; D008954:Models, Biological; D009025:Morpholines / Q000494:pharmacology; D032342:Muscle Cells / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors; D023822:Tissue Engineering; D014665:Vasodilator Agents / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D002867:Chromones; D004791:Enzyme Inhibitors; D005229:Fatty Acids, Monounsaturated; D009025:Morpholines; D014665:Vasodilator Agents; C008757:palmitoleic acid; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D014700:Verapamil