title: The Ala-9Val polymorphism in the mitochondrial targeting sequence (MTS) of the manganese superoxide dismutase gene is not associated with juvenile-onset asthma.
abstract: BACKGROUND
  We aimed to investigate the possible association between Mn-SOD polymorphism in the mitochondrial targeting sequence and asthma.
  
  
  METHODS
  Alanine or valine polymorphism in the signal peptide of Mn-SOD gene was evaluated using a primer pair to amplify a 107-bp fragment followed by digestion with NgoM IV.
  
  
  RESULTS
  No significant difference in genotype frequencies was found between patients and controls.
  
  
  CONCLUSIONS
  These results suggest no major modifying role for the Mn-SOD gene polymorphism in patients with asthma.
authors.affiliation: Department of Biochemistry, Zonguldak Karaelmas University Medical Faculty, Zonguldak, Turkey. dragurel@yahoo.com
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gurel
authors.forename: Nazan
authors.initials: N
authors.lastname: Tomac
authors.forename: H Ramazan
authors.initials: HR
authors.lastname: Yilmaz
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Tekedereli
authors.forename: Omer
authors.initials: O
authors.lastname: Akyol
authors.forename: Ferah
authors.initials: F
authors.lastname: Armutcu
authors.forename: Huseyin
authors.initials: H
authors.lastname: Yuce
authors.forename: Haluk
authors.initials: H
authors.lastname: Akin
authors.forename: Nurten
authors.initials: N
authors.lastname: Ozcelik
authors.forename: Halit
authors.initials: H
authors.lastname: Elyas
mesh_terms: D000293:Adolescent; D017668:Age of Onset; D000409:Alanine / Q000235:genetics*; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005838:Genotype; D006801:Humans; D007223:Infant; D008345:Manganese; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014633:Valine / Q000235:genetics*
chemical_list: D010455:Peptides; D008345:Manganese; D013482:Superoxide Dismutase; D014633:Valine; D000409:Alanine