Improved long-term cryostorage of Escherichia coli competent cells using trehalose.
Trehalose added to storage buffers for chemical-induced transformations at 200 mM increased the survival of Escherichia coli competent cells 1.3 approximately 4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.
Ahn
Taeho
T
Department of Biochemistry, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. thahn@chonnam.ac.kr
Kang
Seong Su
SS
Yun
Chul-Ho
CH
D002470:Cell Survival / Q000187:drug effects*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D014199:Trehalose / Q000494:pharmacology*
D003451:Cryoprotective Agents; D014199:Trehalose