Interaction of BKCa channel modulators with adrenergic agonists in the rat aorta is influenced by receptor reserve.
Our main objective was to study the interaction of BKCa channel modulators with adrenergic agonists UK 14304 and noradrenaline (NA), acting on alpha1-adrenoceptors, in the rat aorta and how this is affected by receptor reserve. NA and UK 14304 evoked concentration-dependent contractions of the rat aorta. UK 14304 was a partial agonist relative to NA in this preparation. The BK(Ca) channel blocker tetraethylammonium (TEA, 1 mM) and opener NS 1619 (3 x 10(-5) M) modulated NA- and UK 14304-induced contractions, and were more effective on UK 14304-induced contractions. TEA (1 mM) increased the maximum response to NA and UK 14304 by about 13% and 300%, respectively, while NS 1619 (3 x 10(-5) M) reduced the maximum response to UK 14304 by about 81% compared to 31% for noradrenaline. The effect of TEA on the noradrenaline concentration-response curve was increased after treatment of the aorta with phenoxybenzamine (PBZ), an irreversible alpha1-adrenoceptor antagonist, to reduce receptor reserve. We concluded that the interaction of BKCa channel modulators with alpha1-adrenergic agonists in the rat aorta was influenced by receptor reserve.
El-Hajj
Hanadi
H
Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait.
Chandrasekhar
Bindu
B
Kadavil
Elizabeth A
EA
Oriowo
Mabayoje A
MA
D000322:Adrenergic Agonists / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D008297:Male; D009638:Norepinephrine / Q000378:metabolism / Q000494:pharmacology; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D011921:Rats, Inbred WKY; D011941:Receptors, Adrenergic / Q000378:metabolism*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology
D000322:Adrenergic Agonists; C494842:Kcnma1 protein, rat; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D015221:Potassium Channels; D011941:Receptors, Adrenergic; D009638:Norepinephrine