title: Matrix-implanted laser desorption/ionization mass spectrometry.
abstract: The implantation of low-velocity massive gold clusters is shown to be a method of choice for homogeneous incorporation of a metallic matrix into the near-surface region of a solid biopolymer for subsequent laser desorption/ionization (LDI) MS analysis. Matrix implanted (MI)LDI spectra from cluster-implanted pure test peptide or tissue exhibit molecular ion peaks similar to those observed by matrix-assisted LDI. Moreover, the ion emission is very reproducible from any spot on the surface of these test samples. MILDI promises to be a powerful technique for mass spectrometric analysis of native biological samples as demonstrated by the first results on rat brain tissues.
authors.affiliation: Institut de Physique Nucléaire, CNRS-IN2P3, 91406 Orsay, France, Equipe Endotoxines, UMR 8619 du CNRS, IBBMC, Université de Paris-Sud, 91405 Orsay, France.
authors.forename: Alexey
authors.initials: A
authors.lastname: Novikov
authors.forename: Martine
authors.initials: M
authors.lastname: Caroff
authors.forename: Serge
authors.initials: S
authors.lastname: Della-Negra
authors.forename: Yvon
authors.initials: Y
authors.lastname: Lebeyec
authors.forename: Michèle
authors.initials: M
authors.lastname: Pautrat
authors.forename: J Albert
authors.initials: JA
authors.lastname: Schultz
authors.forename: Agnès
authors.initials: A
authors.lastname: Tempez
authors.forename: Hay-Yan J
authors.initials: HY
authors.lastname: Wang
authors.forename: Shelley N
authors.initials: SN
authors.lastname: Jackson
authors.forename: Amina S
authors.initials: AS
authors.lastname: Woods
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002138:Calibration; D006046:Gold / Q000737:chemistry; D007328:Insulin / Q000032:analysis / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D007328:Insulin; D010743:Phospholipids; D006046:Gold