title: Structure-activity relationships of acetylcholinesterase noncovalent inhibitors based on a polyamine backbone. 3. Effect of replacing the inner polymethylene chain with cyclic moieties.
abstract: In the present paper we expanded SAR studies of 3, the ethyl analogue of the AChE inhibitor caproctamine (2), by investigating the role of its octamethylene spacer separating the two amide functions through the replacement with dipiperidine and dianiline moieties. Compounds 4 and 8 were the most interesting of the two series of compounds. Compound 4 was the most potent AChE inhibitor with a pIC50 value of 8.48 +/- 0.02, while displaying also significant muscarinic M2 antagonistic activity (pKb value of 6.18 +/- 0.20). The availability of a suitable assay allowed us to verify whether 2, 3, 4, and 8 inhibit AChE-induced Abeta aggregation. Although all four derivatives caused a mixed type of AChE inhibition (active site and PAS), only 4 and 8, which bear an inner constrained spacer, were able to inhibit AChE-induced Abeta aggregation to a greater extent than donepezil. Clearly, the ability of an AChE inhibitor, based on a linear polyamine backbone, to bind both AChE sites may not be a sufficient condition to inhibit also AChE-induced Abeta aggregation. Dipiperidine derivative 4 emerged as a valuable pharmacological tool and a promising lead compound for new ligands to investigate and, hopefully, treat Alzheimer's disease.
authors.affiliation: Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy. vincenzo.tumiatti@unibo.it
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Tumiatti
authors.forename: Vincenza
authors.initials: V
authors.lastname: Andrisano
authors.forename: Rita
authors.initials: R
authors.lastname: Banzi
authors.forename: Manuela
authors.initials: M
authors.lastname: Bartolini
authors.forename: Anna
authors.initials: A
authors.lastname: Minarini
authors.forename: Michela
authors.initials: M
authors.lastname: Rosini
authors.forename: Carlo
authors.initials: C
authors.lastname: Melchiorre
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000737:chemistry; D002091:Butyrylcholinesterase / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D010880:Piperidines / Q000138:chemical synthesis* / Q000737:chemistry*; D011073:Polyamines / Q000138:chemical synthesis* / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: C495227:6-(ethyl-(2-methoxybenzyl)amino)-1-(1'-(6-(ethyl-(2-methoxybenzyl)amino)hexanoyl)-(4,4')bipiperidinyl-1-yl)hexan-1-one; D016229:Amyloid beta-Peptides; D002800:Cholinesterase Inhibitors; D010880:Piperidines; D011073:Polyamines; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase