title: Combined haploid and insertional mutation screen in the zebrafish.
abstract: To identify genes required for development of the brain and somites, we performed a pilot screen of gynogenetic haploid zebrafish embryos produced from mothers mutagenized by viral insertion. We describe an efficient method to identify new mutations and the affected gene. In addition, we report the results of a small-scale screen that identified five genes required for brain development, including novel alleles of nagie oko, pou5f1, ribosomal protein L36, and n-cadherin, as well as a novel allele of the laminin g1 gene that is required for normal skeletal muscle fiber organization and somite patterning.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Wiellette
authors.forename: Yevgenya
authors.initials: Y
authors.lastname: Grinblat
authors.forename: Matthias
authors.initials: M
authors.lastname: Austen
authors.forename: Estelle
authors.initials: E
authors.lastname: Hirsinger
authors.forename: Adam
authors.initials: A
authors.lastname: Amsterdam
authors.forename: Charline
authors.initials: C
authors.lastname: Walker
authors.forename: Monte
authors.initials: M
authors.lastname: Westerfield
authors.forename: Hazel
authors.initials: H
authors.lastname: Sive
mesh_terms: D000483:Alleles; D000818:Animals; D019521:Body Patterning; D001921:Brain / Q000196:embryology; D003001:Cloning, Molecular; D005260:Female; D006162:Guanylate Cyclase / Q000235:genetics; D006238:Haploidy; D008297:Male; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000379:methods*; D009154:Mutation; D010641:Phenotype; D019170:Somites; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D029961:Zebrafish Proteins; C501511:mpp5a protein, zebrafish; D006162:Guanylate Cyclase