Establishment and characterization of four canine melanoma cell lines.
Four canine melanoma cell lines were established from the subcutaneous, oral gingival and mucosal melanoma tissues at the primary and metastatic sites. These cell lines were designated as CMeC-1, CMeC-2, KMeC and LMeC. The cells were spindles in shape, similar to that of primary tumor cells. The doubling times of these cells ranged from 34.1 +/- 5.61 to 57.9 +/- 3.28 hr and their chromosome number ranged from 46 to 80. When transplanted into nude mice, CMeC-1 and LMeC produced tumors, whereas CMeC-2 and KMeC did not. The morphology of the tissue formed by xenotransplantation of these cells was similar to their primary tumors.
Inoue
Kaori
K
Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan
Ohashi
Emi
E
Kadosawa
Tsuyoshi
T
Hong
Sung-Hyeok
SH
Matsunaga
Satoru
S
Mochizuki
Manabu
M
Nishimura
Ryohei
R
Sasaki
Nobuo
N
D000818:Animals; D045744:Cell Line, Tumor / Q000473:pathology*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D008297:Male; D008545:Melanoma / Q000473:pathology / Q000556:secondary / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009062:Mouth Neoplasms / Q000662:veterinary; D009368:Neoplasm Transplantation; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000662:veterinary; D014183:Transplantation, Heterologous