title: Cloning and expression pattern of a novel microspore-specific gene encoding hypersensitive-induced response protein (LjHIR1) from the model legume, Lotus japonicus.
abstract: In order to understand the microspore and pollen development, recently, we have isolated a number of anther-specific genes in the model legume, Lotus japonicus. From these anther-specific genes, we identified one novel microspore-specific gene, LjImfb-c82. In order to determine the molecular characterization of LjImfb-c82, full-length cDNA clone was first isolated and sequenced. It encoded a protein of 286 amino acids (LjHIR1), which had sequence similarity to Hypersensitive-Induced Response like protein. LjHIR1 was specifically expressed in microspore on the in situ hybridization experiment. From the sequence similarity to prohibitin-domain protein, the LjHIR1 might be related to ion channel regulation in microspore development.
authors.affiliation: Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka 020-8550, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hakozaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Endo
authors.forename: Hiromi
authors.initials: H
authors.lastname: Masuko
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Park
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ito
authors.forename: Masanori
authors.initials: M
authors.lastname: Uchida
authors.forename: Motoshi
authors.initials: M
authors.lastname: Kamada
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Higashitani
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D035264:Flowers / Q000235:genetics* / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D000070116:Lotus / Q000235:genetics* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011058:Pollen / Q000502:physiology; D000091402:Prohibitins; D012097:Repressor Proteins / Q000235:genetics
chemical_list: D010940:Plant Proteins; D000091402:Prohibitins; D012097:Repressor Proteins