title: [Extraction and characterization of total DNA from Dendrobium].
abstract: OBJECTIVE
  To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR.
  
  
  METHODS
  the electrophoresis of total DNA, UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods.
  
  
  RESULTS
  The DNA concentration and yields were different, which greatly influenced the result of RAPD-PCR.
  
  
  CONCLUSIONS
  The higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated.
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China. wangyhl1971@yahoo.com
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Song
authors.forename: Quan-sen
authors.initials: QS
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D018744:DNA, Plant / Q000302:isolation & purification*; D031667:Dendrobium / Q000145:classification / Q000235:genetics*; D004586:Electrophoresis; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D011786:Quality Control; D019105:Random Amplified Polymorphic DNA Technique; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002593:Cetrimonium Compounds; D018744:DNA, Plant; D000077286:Cetrimonium