title: Migration of iron oxide-labeled human hematopoietic progenitor cells in a mouse model: in vivo monitoring with 1.5-T MR imaging equipment.
abstract: OBJECTIVE
  To evaluate the use of clinical 1.5-T magnetic resonance (MR) imaging equipment to depict the in vivo distribution of iron oxide-labeled human hematopoietic progenitor cells in athymic mice.
  
  
  METHODS
  This study was approved by the ethical committee, and all women had given consent to donate umbilical cord blood for research. Twenty athymic female Balb/c mice underwent MR imaging before and 1, 4, 24, and 48 hours after intravenous injection of (1-3) x 10(7) human hematopoietic progenitor cells labeled with the superparamagnetic iron oxide particles ferumoxides through simple incubation (n = 10) or P7228 through lipofection (n = 10). Fifteen female Balb/c control mice were examined after intravenous injection of the pure contrast agents (n = 6 for both probes) or nonlabeled cells (n = 3). Signal intensities of liver, spleen, and bone marrow on MR images obtained before and after injection were measured and compared for significant differences by using the t test. MR imaging data were compared with the results of immunostaining against human CD31(+) cells and against the coating of the contrast agents; these results served as the standard of reference.
  
  
  RESULTS
  Ferumoxides was internalized into more mature CD34(-) cells but not into CD34(+) stem cells, while P7228 liposomes were internalized into both CD34(-) and CD34(+) cells. After injection of iron oxide-labeled hematopoietic cells, a significant decrease in MR signal intensity was observed in liver and spleen at 1, 4, 24, and 48 hours after injection (P < .05) and in the bone marrow at 24 and 48 hours after injection (P < .05). The signal intensity decrease in bone marrow was significantly stronger after injection of iron oxide-labeled cells compared to controls that received injections of the pure contrast agent (P < .05). Results of histopathologic examination confirmed homing of iron oxide-labeled human progenitor cells in the murine recipient organs.
  
  
  CONCLUSIONS
  The in vivo distribution of intravenously administered iron oxide-labeled hematopoietic progenitor cells can be monitored with 1.5-T MR imaging equipment.
authors.affiliation: Department of Radiology, Technical University, Munich, Germany. daldrup@radiology.ucsf.edu
authors.forename: Heike E
authors.initials: HE
authors.lastname: Daldrup-Link
authors.forename: Martina
authors.initials: M
authors.lastname: Rudelius
authors.forename: Guido
authors.initials: G
authors.lastname: Piontek
authors.forename: Stephan
authors.initials: S
authors.lastname: Metz
authors.forename: Rosalinde
authors.initials: R
authors.lastname: Bräuer
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Debus
authors.forename: Claire
authors.initials: C
authors.lastname: Corot
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schlegel
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Link
authors.forename: Christian
authors.initials: C
authors.lastname: Peschel
authors.forename: Ernst J
authors.initials: EJ
authors.lastname: Rummeny
authors.forename: Robert A J
authors.initials: RA
authors.lastname: Oostendorp
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000502:physiology; D002465:Cell Movement; D003287:Contrast Media; D003911:Dextrans; D005260:Female; D052203:Ferrosoferric Oxide; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007501:Iron; D008081:Liposomes; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D008807:Mice, Inbred BALB C; D010087:Oxides; D010865:Pilot Projects
chemical_list: D018952:Antigens, CD34; D003287:Contrast Media; D003911:Dextrans; D008081:Liposomes; D058185:Magnetite Nanoparticles; D010087:Oxides; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide