title: Macrophages and mast cells control the neutrophil migration induced by dentin proteins.
abstract: Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), the major dentin proteins, have been shown to induce neutrophil migration through release of IL-1beta, TNF-alpha, MIP-2, and KC. However, the sources of these mediators were not determined. Here, the roles of macrophages and mast cells (MC) in dentin-induced neutrophil accumulation were investigated. Peritoneal MC depletion or the enhancement of macrophage population increased DSP- and DPP-induced neutrophil extravasation. Moreover, supernatants from DSP- and DPP-stimulated macrophages caused neutrophil migration. The release of neutrophil chemotactic factor by macrophages was inhibited by dexamethasone or the supernatant of DSP-treated MC. Consistently, dexamethasone and the MC supernatant inhibited the production of IL-1beta, TNF-alpha, and MIP-2 by macrophages. This inhibitory activity of the DSP-stimulated MC was neutralized by anti-IL-4 and anti-IL-10 antibodies. These results indicate that dentin induces the release of the neutrophil chemotactic substance(s) by macrophages, which are down-modulated by MC-derived IL-4 and IL-10.
authors.affiliation: Department of Stomatology, Faculty of Dentistry of Bauru, University of São Paulo, Brazil. tarcilia@zipmail.com.br
authors.forename: T A
authors.initials: TA
authors.lastname: Silva
authors.forename: V S
authors.initials: VS
authors.lastname: Lara
authors.forename: J S
authors.initials: JS
authors.lastname: Silva
authors.forename: S H P
authors.initials: SH
authors.lastname: Oliveira
authors.forename: W T
authors.initials: WT
authors.lastname: Butler
authors.forename: F Q
authors.initials: FQ
authors.lastname: Cunha
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D054426:Chemokine CXCL2; D018925:Chemokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D003804:Dentin / Q000737:chemistry / Q000502:physiology*; D003907:Dexamethasone / Q000494:pharmacology; D016326:Extracellular Matrix Proteins; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism*; D008264:Macrophages / Q000502:physiology*; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology; D011498:Protein Precursors; D051381:Rats; D012795:Sialoglycoproteins / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D000893:Anti-Inflammatory Agents; D054426:Chemokine CXCL2; D018925:Chemokines; D003907:Dexamethasone; D016326:Extracellular Matrix Proteins; D007375:Interleukin-1; D016209:Interleukin-8; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha; D000099311:Dentin Sialophosphoprotein; C404915:Cxcl2 protein, mouse