Growth and fatty acid composition of Nannochloropsis sp. grown mixotrophically in fed-batch culture.
The cell growth and eicosapentaenoic acid (EPA) yields of Nannochloropsis sp. were enhanced in the fed-batch cultures. With feeding glucose solution, the biomass reached 1.1 g dry wt l(-1) after 10 days' culture, which was 40% higher than that obtained in the batch culture (0.8 g dry wt l(-1)). With supplement of nitrate solution, the biomass reached 1 g dry wt l(-1), and reached the stationary phase 2 days earlier than the others. The maximum of biomass (1.2 g dry wt l(-1)) was obtained with the supplement of the mixture of glucose and nitrate solution. The EPA yields of Nannochloropsis sp. after 10 days' growth in the fed-batch cultures were 52 mg l(-1), 43 mg l(-1) and 56 mg l(-1) with, respectively, addition of nitrate, glucose and both together. In batch culture only 35 mg EPA l(-1) was obtained.
Xu
Fang
F
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, P.O. Box 353, P.R. China.
Cai
Zhao-Ling
ZL
Cong
Wei
W
Ouyang
Fan
F
D000818:Animals; D018533:Biomass; D019149:Bioreactors; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis* / Q000302:isolation & purification; D056890:Eukaryota / Q000254:growth & development* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism
D009566:Nitrates; D015118:Eicosapentaenoic Acid; D005947:Glucose