title: Pulsed electromagnetic fields induce peripheral nerve regeneration and endplate enzymatic changes.
abstract: An experimental study was carried out in rats with the purpose of demonstrating the capacity of pulsed electromagnetic fields (PEMFs) to stimulate regeneration of the peripheral nervous system (PNS). Wistar and Brown Norway (BN) rats were used. Direct sciatic nerve anastomoses were performed after section or allograft interposition. Treatment groups then received 4 weeks of PEMFs. Control groups received no stimulation. The evaluation of the results was carried out by quantitative morphometric analysis, demonstrating a statistically significant increase in regeneration indices (P < 0.05) in the stimulated groups (9000 +/- 5000 and 4000 +/- 6000) compared to the non-stimulated groups (2000 +/- 4000 and 700 +/- 200). An increase of NAD specific isocitrate dehydrogenase (IDH) activity was found along with an increase in the activity of acetyl cholinesterase at the motor plate. The present study might lead to the search for new alternatives in the stimulation of axonal regenerative processes in the PNS and other possible clinical applications.
authors.affiliation: Department of Orthopaedics, University Hospital of Salamanca, Salamanca, Spain. jpedrom@usal.es
authors.forename: J A
authors.initials: JA
authors.lastname: De Pedro
authors.forename: A J
authors.initials: AJ
authors.lastname: Pérez-Caballer
authors.forename: J
authors.initials: J
authors.lastname: Dominguez
authors.forename: F
authors.initials: F
authors.lastname: Collía
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: M
authors.initials: M
authors.lastname: Salvado
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D005260:Female; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D009045:Motor Endplate / Q000201:enzymology* / Q000528:radiation effects* / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology* / Q000528:radiation effects*; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000166:cytology / Q000293:injuries / Q000502:physiology* / Q000528:radiation effects*
chemical_list: D007521:Isocitrate Dehydrogenase; D000110:Acetylcholinesterase