title: Global analysis of protein function using protein microarrays.
abstract: Protein microarrays containing thousands of proteins arrayed at high density can be prepared and probed for a wide variety of activities, thereby allowing the large scale analysis of many proteins simultaneously. In addition to identifying the activities of many previously uncharacterized proteins, protein microarrays can reveal new activities of well-characterized proteins, thus providing new insights about the functions of these proteins. Below, we describe the construction and use of protein microarrays and their applications using yeast as a model system.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Smith
authors.forename: Ghil
authors.initials: G
authors.lastname: Jona
authors.forename: Jason
authors.initials: J
authors.lastname: Ptacek
authors.forename: Geeta
authors.initials: G
authors.lastname: Devgan
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhu
authors.forename: Michael
authors.initials: M
authors.lastname: Snyder
mesh_terms: D000818:Animals; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis*
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins