title: Bone defect repair in rat tibia by TGF-beta1 and IGF-1 released from hydrogel scaffold.
abstract: Bone repair is one of the major challenges facing reconstructive surgery. Bone regeneration is needed for the repair of large defects and fractures. The ability of TGF-beta1 and IGF-1 incorporated into hydrogel scaffold to induce bone regeneration was evaluated in a rat tibia segmental defect model. External fixation was performed prior to the induction of the segmental bone defect in order to stabilize the defect site. Hydrogel scaffold containing either TGF-beta, IGF-1, TGF-beta + IGF-1, hydrogel containing saline or saline, were inserted in the defect. Calcified material was observed in the defects treated with TGF-beta 2 weeks following the start of treatment. Bone defects treated with TGF-beta, IGF-1 or TGF-beta + IGF-1 revealed significant bone formation after 4 and 6 weeks when compared to the control specimens. X-ray images showed that solid bone was present at the defect site after 6 weeks of treatment with TGF-beta or TGF-beta + IGF-1. A less pronounced bone induction was observed in the control specimens and bones treated with IGF-1. Percent closure ratio of bone defects after 6 weeks were 40, 80, 89, and 97% for saline, hydrogel, IGF-1, TGF-beta and IGF-1 + TGF-beta groups, respectively. It is concluded that hydrogel scaffold can serve as a good osteoconductive matrix for growth factors, and that it provides a site for bone regeneration and enhances bone defect healing and could be used as alternative graft material.
authors.affiliation: Oral and Maxillofacial Surgery Department, Carmel Medical Center, Haifa, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Srouji
authors.forename: I
authors.initials: I
authors.lastname: Blumenfeld
authors.forename: A
authors.initials: A
authors.lastname: Rachmiel
authors.forename: E
authors.initials: E
authors.lastname: Livne
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000293:injuries / Q000378:metabolism*; D020100:Hydrogels; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D011859:Radiography; D051381:Rats; D013977:Tibia / Q000000981:diagnostic imaging / Q000293:injuries / Q000378:metabolism; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D020100:Hydrogels; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I