title: [Reconstruction and expression of green fluorescent protein and aquaporin 7 fusion recombinant vector].
abstract: OBJECTIVE
  To study the membrane mobility of aquaporin 7 (AQP7) by cloning stably transfected CHO cells with expression of pEGFP-C1-AQP7, in which AQP7 cDNA was fused downstream and in frame to pEGFP-C1 gene.
  
  
  METHODS
  The full sequence of AQP7 was amplified by RT-PCR and then recombined in the downstream of the green fluorescent protein gene in the pEGFP-C1 vector. The recombinant vector pEGFP-C1-AQP7 was stably transfected into CHO cells. With fluorescent microscopy, immunocytochemical stain and Western blot, pEGFP-C1-AQP7 showed a predominant intracellular vesicular localization.
  
  
  RESULTS
  (1) The sequence of AQP7 cDNA of the Wistar rat was logged into the GenBank (access number: AY157737). (2) Identification demonstrated that pEGFP-C1-AQP7 fusion protein stably expressed in CHO cells. (3) With fluorescence microscopy, pEGFP-C1-AQP7 showed a predominant intracellular vesicular localization.
  
  
  CONCLUSIONS
  The CHO cell line with stable pEGFP-C1-AQP7 expression was set up successfully for advanced research.
authors.affiliation: Department of Reproductive Pathophysiology, School of Medical Sciences, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Danan
authors.initials: D
authors.lastname: Li
authors.forename: Qingshuang
authors.initials: Q
authors.lastname: Liu
authors.forename: Xichun
authors.initials: X
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Xuejian
authors.initials: X
authors.lastname: Zhao
authors.forename: Baoxue
authors.initials: B
authors.lastname: Yang
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000096:biosynthesis* / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*; D014162:Transfection
chemical_list: C114141:AQP7 protein, human; D020346:Aquaporins; D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins