A novel tetrafunctional reagent for simultaneous intramolecular and intermolecular cross-linking of bovine hemoglobin.
A novel tetrafunctional reagent, alpha,gamma,alpha',gamma'-tetra-succinimidyl-hexanediamide-di-glutamate ester (HDG(OSu)(4)), was successfully synthesized, and a well-defined cross-linked bovine hemoglobin (mainly 128 kDa) was prepared with this reagent. Due to the spatial structure of this cross-linking reagent, the intramolecular and intermolecular cross-linking of bovine hemoglobin was formed simultaneously in one reaction. Although the cross-linked bovine hemoglobin showed a slight decrease in half-saturated O(2) pressure value (P(50), from 28.1 mm Hg to 21.7 mm Hg) and Hill coefficient (from 2.5 to 2), due to the cross-linkage, it still performed well for O(2) delivery.
Yun
Qiang
Q
National Laboratory of Biochemical Engineering, Institute of Process Engineering, P.R China.
He
Minglei
M
Xing
Wenchao
W
Bi
Jingxiu
J
Ma
Guanghui
G
Su
Zhiguo
Z
D000818:Animals; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005971:Glutamates / Q000138:chemical synthesis* / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D046912:Multiprotein Complexes / Q000737:chemistry*; D013388:Succinimides / Q000138:chemical synthesis* / Q000737:chemistry*
D003432:Cross-Linking Reagents; D005971:Glutamates; D006454:Hemoglobins; D046912:Multiprotein Complexes; D013388:Succinimides; C500471:alpha,gamma,alpha',gamma'-tetra-succinimidyl-hexanediamide-di-glutamate ester