Intravenous catecholamine administration affects mouse intestinal lymphocyte number and apoptosis.
The purposes of this study were to determine plasma and intestinal epinephrine (E) and norepinephrine (NE) concentrations in mice after exercise stress and, the effect of intravenous injection of E and NE (at concentrations during exercise) on viability of intestinal lymphocytes (IL). Exhaustive exercise significantly elevated plasma E and NE, and intestinal E, compared with sedentary animals. Twenty-four hours after intravenous NE administration, IL counts were higher (p<0.001) and % apoptotic IL were lower (p<0.001) than saline conditions. E resulted in fewer apoptotic IL at 24 h compared to saline controls. E and NE differentially influence IL numbers at 24 h after injection although both result in fewer % apoptotic IL relative to mice given saline only.
Marra
S
S
Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
Burnett
M
M
Hoffman-Goetz
L
L
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D004837:Epinephrine / Q000097:blood / Q000633:toxicity*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D007275:Injections, Intravenous / Q000379:methods; D007422:Intestines / Q000166:cytology*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000097:blood / Q000633:toxicity*; D010641:Phenotype; D010805:Physical Conditioning, Animal / Q000379:methods; D011419:Propidium; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011897:Random Allocation; D014018:Tissue Distribution
D017304:Annexin A5; D019253:Proto-Oncogene Proteins c-bcl-2; D011419:Propidium; D017493:Leukocyte Common Antigens; D009638:Norepinephrine; D004837:Epinephrine