Radioprotection of DNA by glycyrrhizic acid through scavenging free radicals.
Gamma-radiation induced strand breaks in plasmid pBR322 DNA. Glycyrrhizic acid (GZA) protected plasmid DNA from radiation-induced strand breaks, as the disappearance of super-coiled (ccc) form was prevented by the compound with a dose-reduction factor of 2.04 at 2.5 mM concentration. Studies of comet assay on human peripheral blood leukocytes exposed to gamma radiation in the presence and absence of glycyrrhizic acid ex vivo revealed that this compound protected the cellular DNA from radiation-induced strand breaks in a concentration-dependent manner. An intraperitoneal administration of the GZA to mice one hour before exposure to gamma radiation protected cellular DNA from radiation-induced strand breaks in peripheral blood leucocytes and bone marrow cells, as revealed by comet assay. Pulse radiolysis studies indicated that glycyrrhizic acid offered radioprotection by scavenging free radicals. The rate constants for the reaction of glycyrrhizic acid with OH* and e(aq)- are 1.2 x 10(10 ) M(-1) s(-1) and 3.9 x 10(9 ) M(-1) s(-1), respectively.
Gandhi
Nitin Motilal
NM
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India.
Maurya
Dharmendra Kumar
DK
Salvi
Veena
V
Kapoor
Sudhir
S
Mukherjee
Tulsi
T
Nair
Cherupally Krishnan K
CK
D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D020552:Comet Assay; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects*; D004249:DNA Damage; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005720:Gamma Rays; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D011675:Pulse Radiolysis; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D016166:Free Radical Scavengers; D011837:Radiation-Protective Agents; D019695:Glycyrrhizic Acid; D004247:DNA