Estrogen receptor alpha expression in mice kidney shows sex differences during aging.
We have used semi quantitative RT-PCR and western blotting for the analysis of expression of estrogen receptor (ER) alpha and beta mRNA and protein in the kidney of adult and old mice of both sexes. Uterus, a well-known target for estrogen action, was used for comparison. As compared to adult, the expression of both ER alpha mRNA and protein of old mice decreased in male but increased in female. However, unlike uterus, neither ERbeta mRNA nor protein could be detected in the kidney of adult or old mice. Thus the present data reveal that the expression of ERalpha in mice kidney shows sex differences during aging.
Sharma
P K
PK
Biochemistry and Molecular Biology Laboratory, Center of Advanced Study in Zoology, Banaras Hindu University, Varanasi 221005, India.
Thakur
M K
MK
D000375:Aging / Q000378:metabolism*; D000818:Animals; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D012737:Sex Factors; D014599:Uterus / Q000378:metabolism
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger