[The effects of si-wu-tang on serum protein of blood deficient mice induced by radiation].
OBJECTIVE
To study the effects of Si-Wu-Tang on serum protein of blood deficient mice b y proteomicstechnique and study the enriching and regulating blood mechanism of Si-Wu-Tang on mocular level.


METHODS
The blood deficient mice was induced by using a single dose of 3.5 Gy radiation from a 60Cogamma source, and high resolution two-dimensional polyacryamide gel electrophoresis (2-DE), computer-assisted image analysis, and mass spectrometry were used to detect regulated protein by Si-Wu-Tang.


RESULTS
12 lower and 4 higher protein in sera could be recovered by Si-Wu-Tang, 4 protein might be DNA-dependent protein kinase catalytic subunit, Dystrophin, KIF13A, dystonin. They play a part in DNA double-stranded break repair, recombination and modulation of transcription, transportation of mannose-6-phosphate receptor, etc.


CONCLUSIONS
Si-Wu-Tang can regulate serum protein in blood deficient mice, resulting in improving hematopoiesis and lessening irradiated injury.
Ma
Zeng-chun
ZC
Institute of Radiation Medicine, Beijing 100850, China.
Gao
Yue
Y
Tang
Hong-ling
HL
Wang
Sheng-qi
SQ
D000818:Animals; D001324:Autoantigens / Q000097:blood; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D000071821:Dystonin; D016189:Dystrophin / Q000097:blood; D005260:Female; D016547:Kinesins / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens / Q000097:blood; D010946:Plants, Medicinal / Q000737:chemistry; D011833:Radiation Injuries, Experimental / Q000097:blood*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014916:Whole-Body Irradiation; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000071821:Dystonin; D016189:Dystrophin; D016547:Kinesins; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D011837:Radiation-Protective Agents; D000097805:Collagen Type XVII; C486268:DST protein, human; C417670:KIF13A protein, human; C403258:si-wu-tang