title: Expeditor: a pipeline for designing primers using human gene structure and livestock animal EST information.
abstract: We have developed software, called Expeditor, that can be used to combine known gene structure information from human and coding sequence information from farm animal species for a streamlined primer design in target farm animal species. This software has many utilities, which include PCR-based SNP discovery for identification of genes/markers associated with economically important traits in farm animals, comparative mapping analysis, and evolution studies. The use of this software helps minimize tedious manual operations and reduces the chance of errors by more conventional approaches.
authors.affiliation: Department of Animal Science, 2255 Kildee Hall, Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011, USA. zhu@iastate.edu
authors.forename: Z-L
authors.initials: ZL
authors.lastname: Hu
authors.forename: K
authors.initials: K
authors.lastname: Glenn
authors.forename: A M
authors.initials: AM
authors.lastname: Ramos
authors.forename: C J
authors.initials: CJ
authors.lastname: Otieno
authors.forename: J M
authors.initials: JM
authors.lastname: Reecy
authors.forename: M F
authors.initials: MF
authors.lastname: Rothschild
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D017931:DNA Primers; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D015894:Genome, Human; D006801:Humans; D016133:Polymerase Chain Reaction; D012984:Software
chemical_list: D017931:DNA Primers