[Cloning and expression of aldolase encoding gene of Plasmodium falciparum FCC1/HN strain].
OBJECTIVE
To clone, sequence, and express the aldolase (ALD) encoding gene of Plasmodium falciparum FCC1/HN strain.


METHODS
The ALD encoding gene was amplified by PCR from genomic DNA of FCC1/HN strain. The positive clones were screened and identified by agarose gel electrophoresis and endonuclease. The recombinant plasmid was transformed into E. coli M15. The fusion protein was expressed by IPTG induction and purified by Ni-NTA affinity chromatography and anion exchange column.


RESULTS
The ALD gene of P. falciparum was amplified. Analysis of sequencing showed that the ALD gene of P. falciparum was identical with the sequence of other reported isolates. A Mr 41,000 fusion protein was induced by IPTG and was purified by chromatography.


CONCLUSIONS
The ALD gene of P. falciparum FCC1/HN strain was identical to the other reported isolates. ALD fusion protein of P. falciparum was expressed and purified.
Zhang
Rui-juan
RJ
Department of Pathogenic Biology, The Second Military Medical University, Shanghai 200433, China.
Zhu
Huai-min
HM
Cao
Yi
Y
Zhou
Ai-guo
AG
Zheng
Zhi-qiang
ZQ
Zhang
Qing-feng
QF
Zheng
Hui
H
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014170:Transformation, Genetic
D011993:Recombinant Fusion Proteins; D005634:Fructose-Bisphosphate Aldolase