Intraplatelet cyclic guanosine-3',5'-monophosphate levels during pregnancy and preeclampsia.
OBJECTIVE
To investigate the intraplatelet cyclic guanosine-3',5'-monophosphate (cGMP) levels during normal pregnancy and preeclampsia.


METHODS
Pregnant women (n = 15), women with preeclampsia (n = 15), and nonpregnant, normotensive women (n = 15) were included. Intraplatelet cyclic guanosine-3',5'-monophosphate levels were measured by an enzyme-linked immunosorbent assay.


RESULTS
Intraplatelet cGMP levels were significantly different among all groups (p < 0.02). The values were higher in normal pregnant women (mean 19.8 SD 2.6 fmol/10(5) platelets) in comparison to nonpregnant women (mean 7.6 SD 0.3 fmol/10(5)platelets; p = 0.001) and women with preeclampsia (mean 11.3 SD 1.8 fmol/10(5) platelets; p = 0.05). Plasma nitric oxide levels did not reveal differences between all groups.


CONCLUSIONS
The results of this study in a high-risk Andean population demonstrated that intraplatelet cyclic guanosine-3',5'-monophosphate levels are decreased during preeclampsia compared to normal pregnancy, suggesting a lack in action of nitric oxide.
Teran
Enrique
E
Experimental Pharmacology and Cellular Metabolism Unit, Biomedical Center, Central University of Ecuador, Quito, Ecuador. eteran@ucentral.edu.ec
Escudero
Carlos
C
Vivero
Sandra
S
Enriquez
Armando
A
Calle
Andres
A
D000328:Adult; D001792:Blood Platelets / Q000201:enzymology*; D006152:Cyclic GMP / Q000097:blood*; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000097:blood; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy / Q000097:blood*
D009569:Nitric Oxide; D006152:Cyclic GMP