title: Analysis of serum soluble CD40 ligand (sCD40L) in the patients undergoing allogeneic stem cell transplantation: platelet is a major source of serum sCD40L.
abstract: CD40 ligand (CD40L) is expressed not only on activated T cells but also on activated platelets. A soluble CD40 ligand (sCD40L) is released from the activated T cells and platelets by ill-defined proteolytic process in vitro. It has been reported that sCD40L is elevated in the serum of patients with systemic lupus erythematosus, unstable angina, essential thrombocythemia, and autoimmune thrombocytopenic purupura. However, source of sCD40L in vivo remains to be elucidated. We investigated the serial sCD40L in the serum in patients undergoing allogeneic stem cell transplantation and compared with the platelets number and soluble IL2R, which is a marker of activated T cells. The value of sCD40L was well correlated with platelet number or thrombopoiesis. In cases of severe graft vs. host disease with markedly increased sIL2R, sCD40L was not increased in vivo. These results indicate that sCD40L in vivo is released mainly from the platelets or in the process of platelet production but not from the activated T cells.
authors.affiliation: Department of Pediatrics and Developmental Biology, Postgraduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan. mnagasawa.ped@tmd.ac.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nagasawa
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Isoda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tomizawa
authors.forename: Sukeyuki
authors.initials: S
authors.lastname: Itoh
authors.forename: Michiko
authors.initials: M
authors.lastname: Kajiwara
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Morio
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Nonoyama
authors.forename: Norio
authors.initials: N
authors.lastname: Shimizu
authors.forename: Shuki
authors.initials: S
authors.lastname: Mizutani
mesh_terms: D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000276:immunology*; D023201:CD40 Ligand / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D036101:Cord Blood Stem Cell Transplantation; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D006942:Hypergammaglobulinemia / Q000097:blood / Q000628:therapy; D007075:Immunoglobulin M; D007223:Infant; D008213:Lymphocyte Activation; D036102:Peripheral Blood Stem Cell Transplantation; D010976:Platelet Count; D015375:Receptors, Interleukin-2 / Q000097:blood; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology; D034061:Thrombopoiesis; D014184:Transplantation, Homologous
chemical_list: D007075:Immunoglobulin M; D015375:Receptors, Interleukin-2; D023201:CD40 Ligand