[Establishment of tumor cell membrane model and screening for specific anti-tumor peptides].
OBJECTIVE
Some polypeptides have targeting selective anti-tumor effects act on cell membrane, but studies on this field were restricted by lack of peptide information. This study was to establish a tumor cell membrane model, and isolate specific anti-tumor peptides.


METHODS
Pore-forming peptides Mast21 and MastoparanX were chosen as positive control peptides to optimize the artificially synthesized tumor liposome cell membrane model, ribosome display system was used to isolate specific anti-tumor peptides from a designed random DNA library with affinity to membrane model. Anti-tumor effects of the isolated peptides on non-small cell lung cancer cell line NCI-H460 were analyzed with MTT assay.


RESULTS
After 6-round successive selection with improved membrane model (PE/PS, 3:7) and ribosome display system, some sequences of peptides were obtained. In vitro experiment confirmed that some of them showed anti-tumor effects on NCI-H460 cells, 50% inhibitory concentration (IC(50)) of peptides SeqA3, and SeqB3 of NCI-H460 cells were (22.5+/-1.2) micromol/L,and (11.3+/-0.7) micromol/L, while on normal human fibro cell line CCD-27SK were more than 100 micromol/L.


CONCLUSIONS
Specific anti-tumor peptides may be screened out by suitable tumor cell membrane model and ribosome display system, which will help to develop new anti-tumor drugs.
Wen
Zhe-Sheng
ZS
Department of Thoracic Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, P.R. China.
Rong
Tie-Hua
TH
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D020128:Inhibitory Concentration 50; D008081:Liposomes; D008175:Lung Neoplasms / Q000473:pathology*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism*
D000970:Antineoplastic Agents; D008081:Liposomes; D019151:Peptide Library; D010455:Peptides