title: Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use.
abstract: Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect.
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Hyeong-Jun
authors.initials: HJ
authors.lastname: Lim
authors.forename: So-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Wan-Kyu
authors.initials: WK
authors.lastname: Lee
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D001644:Bifidobacterium / Q000302:isolation & purification* / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000302:isolation & purification* / Q000502:physiology; D008297:Male; D019936:Probiotics / Q000627:therapeutic use*; D013291:Streptococcus / Q000302:isolation & purification* / Q000502:physiology; D013657:Taurodeoxycholic Acid
chemical_list: D013657:Taurodeoxycholic Acid; D002784:Cholesterol; D000581:Amidohydrolases; C023154:choloylglycine hydrolase