title: Gene discovery in Boophilus microplus, the cattle tick: the transcriptomes of ovaries, salivary glands, and hemocytes.
abstract: The quest for new control strategies for ticks can profit from high throughput genomics. In order to identify genes that are involved in oogenesis and development, in defense, and in hematophagy, the transcriptomes of ovaries, hemocytes, and salivary glands from rapidly ingurgitating females, and of salivary glands from males of Boophilus microplus were PCR amplified, and the expressed sequence tags (EST) of random clones were mass sequenced. So far, more than 1,344 EST have been generated for these tissues, with approximately 30% novelty, depending on the the tissue studied. To date approximately 760 nucleotide sequences from B. microplus are deposited in the NCBI database. Mass sequencing of partial cDNAs of parasite genes can build up this scant database and rapidly generate a large quantity of useful information about potential targets for immunobiological or chemical control.
authors.affiliation: Center for Genetic Resources and Biotechnology, Empresa Brasileira de Pesquisa Agropecuária, Brasília, DF, Brazil. imsantos@rpm.fmrp.usp.br
authors.forename: Isabel K F de Miranda
authors.initials: IK
authors.lastname: Santos
authors.forename: Jesus G
authors.initials: JG
authors.lastname: Valenzuela
authors.forename: José Marcos C
authors.initials: JM
authors.lastname: Ribeiro
authors.forename: Marilia
authors.initials: M
authors.lastname: de Castro
authors.forename: Juliana Nardelli
authors.initials: JN
authors.lastname: Costa
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Costa
authors.forename: Edson Ramiro
authors.initials: ER
authors.lastname: da Silva
authors.forename: Olavo Bilac Rego
authors.initials: OB
authors.lastname: Neto
authors.forename: Clarisse
authors.initials: C
authors.lastname: Rocha
authors.forename: Sirlei
authors.initials: S
authors.lastname: Daffre
authors.forename: Beatriz R
authors.initials: BR
authors.lastname: Ferreira
authors.forename: João Santana
authors.initials: JS
authors.lastname: da Silva
authors.forename: Matias Pablo
authors.initials: MP
authors.lastname: Szabó
authors.forename: Gervasio Henrique
authors.initials: GH
authors.lastname: Bechara
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D018076:DNA, Complementary / Q000032:analysis*; D030541:Databases, Genetic; D005260:Female; D015723:Gene Library; D006434:Hemocytes; D008297:Male; D009866:Oogenesis / Q000235:genetics*; D010053:Ovary; D016133:Polymerase Chain Reaction; D012469:Salivary Glands; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D018076:DNA, Complementary