Fluorescence enhancements on silver colloid coated surfaces.
We observed a strong, more than 16-fold, enhancement of Texas Red-labeled BSA fluorescence emission when deposited on silver colloid coated surfaces (SCCS). The same labeled protein deposited on silver island films (SIFs) showed an approximate 8-fold fluorescence enhancement. The lifetimes of Texas Red-BSA fluorescence are significantly shorter on silvered surfaces than on uncoated quartz substrate indicating a strong change in radiative decay rate of the dyes. We also observed a 36-fold increased brightness of overlabeled fluorescein-HSA deposited on silver colloid coated surface. Stronger enhancement observed for overlabeled Fl-HSA protein indicates that presence of silver particles partially decreased self-quenching. Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence.
Lukomska
Joanna
J
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
Malicka
Joanna
J
Gryczynski
Ignacy
I
Lakowicz
Joseph R
JR
D000465:Algorithms; D003102:Colloids / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005453:Fluorescence; D011791:Quartz / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014966:Xanthenes / Q000737:chemistry
D003102:Colloids; D012709:Serum Albumin; D014966:Xanthenes; D011791:Quartz; D012710:Serum Albumin, Bovine; D012834:Silver; C034657:Texas red; D016650:Fluorescein-5-isothiocyanate