Morphogenetic pathway of spore wall assembly in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae spore is protected from environmental damage by a multilaminar extracellular matrix, the spore wall, which is assembled de novo during spore formation. A set of mutants defective in spore wall assembly were identified in a screen for mutations causing sensitivity of spores to ether vapor. The spore wall defects in 10 of these mutants have been characterized in a variety of cytological and biochemical assays. Many of the individual mutants are defective in the assembly of specific layers within the spore wall, leading to arrests at discrete stages of assembly. The localization of several of these gene products has been determined and distinguishes between proteins that likely are involved directly in spore wall assembly and probable regulatory proteins. The results demonstrate that spore wall construction involves a series of dependent steps and provide the outline of a morphogenetic pathway for assembly of a complex extracellular structure.
Coluccio
Alison
A
Life Sciences, SUNY Stony Brook, Stony Brook, NY 11794-5215, USA.
Bogengruber
Edith
E
Conrad
Michael N
MN
Dresser
Michael E
ME
Briza
Peter
P
Neiman
Aaron M
AM
D002462:Cell Membrane / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry; D004252:DNA Mutational Analysis; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005838:Genotype; D005944:Glucosamine / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D012680:Sensitivity and Specificity; D013172:Spores, Fungal / Q000737:chemistry / Q000502:physiology*; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D047071:beta-Glucans / Q000737:chemistry
D005656:Fungal Proteins; D047071:beta-Glucans; D014443:Tyrosine; D048271:Chitosan; C007543:dityrosine; D001616:beta-Galactosidase; D005944:Glucosamine