Amelioration of genotoxic damage by certain phytoproducts in human lymphocyte cultures.
The antigenotoxic effect of some phytoproducts like carotenoid (beta-carotene), curcumin, ascorbic acid and flavonoid (genistein)was demonstrated on the genotoxicity induced by hydrocortisone. Human lymphocyte cultures were studied for the induction of chromosomal aberrations, sister chromatid exchanges and effect on cell cycle kinetics with or without the presence of metabolic activation (S9 mix). The phytoproducts were studied in two most effective doses viz. carotenoid (0.5 and 0.7 microM), curcumin (15 and 25 microM), ascorbic acid (60 and 80 microM) and flavonoid (25 and 40 microM) in 24, 48 and 72 h cultures, and they were found to reduce chromosomal aberrations, sister chromatid exchange and increase replication index. The present study showed that the ascorbic acid and curcumin were more effective than carotenoid and flavonoid, though all provide protection against the genotoxicity of hydrocortisone.
Ahmad
Md Sultan
MS
Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202002 India.
Sheeba
Afzal
Mohd
M
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002453:Cell Cycle / Q000502:physiology; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003474:Curcumin / Q000494:pharmacology; D004347:Drug Interactions; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000037:antagonists & inhibitors / Q000633:toxicity*; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012854:Sister Chromatid Exchange / Q000187:drug effects; D019207:beta Carotene / Q000494:pharmacology
D016587:Antimutagenic Agents; D000975:Antioxidants; D009153:Mutagens; D019207:beta Carotene; D019833:Genistein; D003474:Curcumin; D001205:Ascorbic Acid; D006854:Hydrocortisone