[Advanced oxidation protein products induce reactive oxygen species production in endothelial cells].
OBJECTIVE
To investigate the effect of advanced oxidation protein products (AOPP) on production of reactive oxygen species (ROS) in endothelial cells.


METHODS
Human umbilical vein endothelial cell line ECV304 were stimulated with AOPP-modified bovine serum albumin (AOPP-BSA) prepared by oxidation of BSA with HOCl in vitro. ROS production in the cells was evaluated by kinetic measurement of dichlorofluoroscein (DCF) fluorescence produced by oxidation of an oxidant-sensitive dye 2,7-dichlorefluorescin (DCFH) using VICTOR 1420 multilabel counter.


RESULTS
AOPP-BSA could induce ROS production in ECV304 cells time- and dose-dependently, and the quantity of ROS increased with the oxidation degree of BSA. Pretreatment of cells with a small-molecular-mass glutathione peroxidase mimic ebselen or NADPH oxidase inhibitor apocynin inhibited ROS production by 65% and 29% respectively.


CONCLUSIONS
AOPP-BSA induces ROS production in endothelial cells, partially mediated by NADPH oxidase activation.
Yuan
Fang
F
Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Liu
Shang-xi
SX
Tian
Jian-wei
JW
D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D019255:NADPH Oxidases / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011506:Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
D011506:Proteins; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D019255:NADPH Oxidases