title: Truncated and RIP-degenerated copies of the LTR retrotransposon Pholy are clustered in a pericentromeric region of the Leptosphaeria maculans genome.
abstract: The LMR1 5.2 kb interspersed repeat of Leptosphaeria maculans was described by Taylor and Borgmann [Mol. Plant Microbe Interact. 7 (1994) 181] as an uncharacterized repeated element sharing homologies with both LINEs and SINEs. Here, we used the LMR1 sequence as a template to identify the full-length element within a 184-kb genomic sequence corresponding to the pericentromeric region of the 2.80 Mb chromosome of isolate v23.1.3. This region comprises (i) one 6980-bp full-sized Pholy element bordered by two 275- to 280-bp long terminal repeats (LTRs), (ii) five Pholy-related sequences, usually truncated at their 3' ends, and (iii) five solo-LTRs. Structural features strongly suggested that Pholy corresponds to an ancient copia-like retrotransposon, sharing strong homologies with the Elsa retrotransposon of Stagonospora nodorum. Pholy was also suggested to be specific to pericentromeric regions. Comparative analysis of the structure of the Pholy-like sequences occurring in the 184-kb contig and in other parts of the genome showed that this family of repeats is highly degenerated following extensive repeat induced point mutation (RIP).
authors.affiliation: INRA, PMDV, Route de Saint Cyr, F-78026 Versailles Cedex, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Attard
authors.forename: Lilian
authors.initials: L
authors.lastname: Gout
authors.forename: Simon
authors.initials: S
authors.lastname: Ross
authors.forename: Francis
authors.initials: F
authors.lastname: Parlange
authors.forename: Laurence
authors.initials: L
authors.lastname: Cattolico
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Balesdent
authors.forename: Thierry
authors.initials: T
authors.lastname: Rouxel
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics*; D002503:Centromere; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D005800:Genes, Fungal; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D017354:Point Mutation; D018626:Retroelements / Q000235:genetics*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences / Q000235:genetics*
chemical_list: D018626:Retroelements