[Expression of NGF, BDNF and NT-3 in satellite cells of dorsal root ganglion].
OBJECTIVE
To investigate the distribution of NGF, BDNF, NT-3 and their mRNA in satellite cells of dorsal root ganglion (DRG) in adult cat.


METHODS
Five male adult cats were used and their L6 DRGs unilateral side were taken and sectioned into frozen sections 20 microm in thickness respectively. The sections were stained by immunohistochemistry ABC method (NGF antibody 1:1000, Sigma; BDNF antibody 1:500, Santa Cruz; NT-3 antibody 1:1500, Santa Cruz), and in situ hybridization study(cRNA probe 1:100).


RESULTS
NGF, BDNF, NT-3 and their mRNA were observed in some satellite cells. The positive signal of mRNA for NGF, BDNF and NT-3 was observed in cytoplasm. However, there was difference in the subcellular localization of protein for the three factors. BDNF immunoreactivity (BDNF-IR) was located in cytoplasm, while NGF-IR and NT-3-IR were located in nucleus.


CONCLUSIONS
The results demonstrated that some satellite cells contain NGF, BDNF and NT-3 as well as their mRNA. The difference of subcellular localization for NGF, BDNF and NT-3 indicated that the functional mechanism of each factor in satellite cells in adult cats may be different.
Liu
Fen
F
Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
Wang
Tinghua
T
Li
Ming
M
Wu
Linyan
L
Feng
Zhongtang
Z
Wang
Tewei
T
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D002415:Cats; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D020933:Neurotrophin 3 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D027161:Satellite Cells, Perineuronal / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D020933:Neurotrophin 3; D012333:RNA, Messenger; D020932:Nerve Growth Factor