title: [Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis].
abstract: OBJECTIVE
  To establish an in vitro model for the development of mouse spermatogenic cells into sperm by using the immunodefective mouse as the incubator.
  
  
  METHODS
  Tissue grafting was performed using testis from 1-2 days old Kun-ming mice as donor tissue and immunodefective mice as recipients; the expression of TESK1 mRNA in grafts was determined by RT-PCR and the spermatogenesis further observed with histological analysis of grafts.
  
  
  RESULTS
  Molecular biological and histological analyses showed that grafts post-grafting not only expressed TESK1 mRNA as in normal mouse testis, but also exhibited similarities in the structure of seminiferous tubules and component of spermatogenic cells, including sperms.
  
  
  CONCLUSIONS
  Spermatogenic cells heterotopically grafted in vitro could continuously grow and complete spermatogenesis and finally develop into sperm.
authors.affiliation: Laboratory of Male Reproduction, Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518036, China. yujie007@hotmail.com
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.forename: Fang-ting
authors.initials: FT
authors.lastname: Zhang
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Wan
authors.forename: Mei-jun
authors.initials: MJ
authors.lastname: Yin
authors.forename: Jia-zhi
authors.initials: JZ
authors.lastname: Fang
authors.forename: Yao-ting
authors.initials: YT
authors.lastname: Gui
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Cai
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D023421:Models, Animal; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000378:metabolism / Q000637:transplantation*; D016042:Transplantation, Heterotopic
chemical_list: D012333:RNA, Messenger; C097507:testis-specific protein kinase 1; D017346:Protein Serine-Threonine Kinases