Immunocytochemical localisation of calreticulin in Trypanosoma cruzi.
Calreticulin, a Ca(2+) chaperone, is found in many different locations in various eukaryotic cells, including lumen of the endoplasmic reticulum, the cell surface, perinuclear areas and cytosolic granules. In the present study, a polyclonal antibody against calreticulin was used for the immunocytochemical localisation of the protein in Trypanosoma cruzi. Labelling was observed in the endoplasmic reticulum, Golgi complex, reservosomes, flagellar pocket, cell surface, cytosol, nucleus and kinetoplast. Significant differences in labelling were observed among the three evolutive forms of the protozoan. The functional role of calreticulin in T. cruzi is discussed.
Souto-Padrón
Thaïs
T
Laboratório de Biologia Celular e Ultraestrutura, Instituto de Microbiologia Centro de Ciências da Saúde, Bloco I, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, CEP 21949-590 Rio de Janeiro, Brazil. wsouza@biof.ufrj.br
Labriola
Carlos A
CA
de Souza
Wanderley
W
D000818:Animals; D037282:Calreticulin / Q000276:immunology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D018105:DNA, Kinetoplast / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000378:metabolism* / Q000648:ultrastructure
D037282:Calreticulin; D018105:DNA, Kinetoplast