Quantitation and visualization of tumor-specific T cells in the secondary lymphoid organs during and after tumor elimination by PET.
Increased understanding in the area of trafficking behavior of adoptively transferred tumor-specific T cells could help develop better therapeutic protocols. We utilized the DUC18/CMS5 tumor model system in conjunction with a microPET scanner to study the DUC18 T cell distribution pattern in spleens and lymph nodes in live mice. Anti-Thy1.2 antibodies conjugated to 1,4,7,10-tetraazacyclododecane-N,N',N'',N''-tetraacetic acid (DOTA) and radiolabeled with (64)Cu were administered to three groups of BALB-Thy1.1 mice on days 4, 7, or 14 post-DUC18 T cell transfer. We were able to detect the transferred cells in all the major lymph nodes, spleens, and in tumors. Our findings suggest that tumor-specific T cells do not all preferentially localize to the tumors but they also home to all the major lymphoid organs; additionally the number of DUC18 T cells remains relatively constant during and after tumor elimination within each lymphoid organ.
Matsui
Ken
K
Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave., St. Louis, MO 63110, USA.
Wang
Zheng
Z
McCarthy
Timothy J
TJ
Allen
Paul M
PM
Reichert
David E
DE
D000818:Animals; D045744:Cell Line, Tumor; D005354:Fibrosarcoma / Q000000981:diagnostic imaging* / Q000556:secondary / Q000628:therapy*; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D008207:Lymphatic Metastasis; D051379:Mice; D008807:Mice, Inbred BALB C; D049268:Positron-Emission Tomography / Q000379:methods*; D011379:Prognosis; D013154:Spleen / Q000000981:diagnostic imaging*; D013601:T-Lymphocytes / Q000000981:diagnostic imaging* / Q000637:transplantation*; D016896:Treatment Outcome