Emerging importance of Pneumocystis carinii among indian immunosuppressed patients.
Pneumocystis carinii is known to cause both significant morbidity and mortality in immunosuppressed individuals. In a six and a half year period starting with 1992, a total of 204 samples including bronchial aspirates, induced sputum and suction catheter tips were examined for P. carinii by direct microscopic examination of Giemsa's and toluidine 'O' stained smears. At a later stage of the investigation, immunofluorescent staining using monoclonal reagent (Meriflour, USA) was also used for examination of the specimens. In all 24 (11.8%) of 204 samples were positive for P. carinii. In addition, Pneumocystis carinii pneumonia (PCP) was diagnosed in five (33%) of 15 patients of acquired immunodeficiency syndrome (AIDS) with opportunistic infections. All these 15 patients had CD4 T cell counts of less than 500 T lymphocyte equivalent (TLE) /ml as measured by a Trax ELISA assay. Laboratories in India have to be better equipped for an early and correct diagnosis of PCP that is bound to rise with the increase in the number and variety of immunosuppressed patients.
Mathews
M S
MS
Department of Microbiology, CMC Hospital, Vellore-632 004, India. mmathews@micro.cmc.ernet.in
Mathai
E
E
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016867:Immunocompromised Host; D007194:India; D045363:Pneumocystis carinii / Q000302:isolation & purification* / Q000472:pathogenicity; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis / Q000473:pathology*; D012307:Risk Factors