[Effect of HIV Tat protein on CCR5 expression in monocytes and infection with monocyte-tropic HIV strains].
To study the effects of HIV Tat protein on CCR5 expression of monocytes and HIV infection in monocytes.



Membrane expression of CCR5 on monocytes was analyzed by flow cytometry. Stimulated with HIV Tat protein, monocytes were infected with monocyte-tropic HIV(Ba-L) and HIV gag p24 level in the supernatant was measured by ELISA methods.



HIV Tat protein increased CCR5 expression in human monocytes,which was inhibited by rabbit anti-Tat polyclonal antibody. Tat protein also increased p24 level after monocyte-tropic HIV-1(Ba-L) infected monocytes.



Tat increases CCR5 expression and HIV-1 infection in monocytes, which indicates that HIV Tat might be a key protein in HIV-1 infection.
Yang
Yi-da
YD
Department of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China. yidayang@hotmail.com
Zheng
Lin
L
Lu
Guo-cai
GC
Chen
Ya-gang
YG
Salvato
Maria
M
D005434:Flow Cytometry; D015696:Gene Products, tat / Q000494:pharmacology*; D006678:HIV; D015658:HIV Infections / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D019713:Receptors, CCR5 / Q000096:biosynthesis* / Q000235:genetics; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D019713:Receptors, CCR5; D054322:tat Gene Products, Human Immunodeficiency Virus