Essential role of the small subunit of thermostable glucose dehydrogenase from Burkholderia cepacia.
The co-expression in Escherichia coli of the gamma-subunit and the catalytic alpha-subunit of the thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia sp. SM4 produced 12.7 U GDH activity mg(-1) protein. A 47-amino acid, twin-arginine translocase signal peptide was identified at the amino terminus of the gamma-subunit. The expression of the alpha-subunit in the absence of the gamma-subunit or the gamma-subunit signal peptide failed to produce any detectable GDH protein or activity. The gamma-subunit may be a chaperone-like component that assists folding of the alpha-subunit polypeptide to the active form and its translocation to the periplasm.
Yamaoka
Hideaki
H
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-13 Naka-machi, Koganei, Tokyo, 184-8588, Japan.
Ferri
Stefano
S
Fujikawa
Masako
M
Sode
Koji
K
D000595:Amino Acid Sequence; D016956:Burkholderia cepacia / Q000201:enzymology* / Q000235:genetics; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D042843:Glucose 1-Dehydrogenase / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D013696:Temperature
D021122:Protein Subunits; D011994:Recombinant Proteins; D042843:Glucose 1-Dehydrogenase