Characterization of an ExoS Type III translocation-resistant cell line.
Pseudomonas aeruginosa ExoS is a type III-secreted type III-secreted, bifunctional protein that causes diverse effects on eukaryotic cell function. The coculture of P. aeruginosa strains expressing ExoS with HL-60 myeloid cells revealed the cell line to be resistant to the toxic effects of ExoS. Differentiation of HL-60 cells with phorbol 12-myristate 13-acetate (TPA) rendered the cell line sensitive to ExoS. To understand the cellular basis for the alteration in sensitivity, undifferentiated and TPA-differentiated HL-60 cells were compared for differences in bacterial adherence, type III secretion induction, and ExoS translocation. These comparisons found that ExoS was translocated more efficiently in TPA-differentiated HL-60 cells than in undifferentiated cells. The studies support the ability of eukaryotic cells to influence P. aeruginosa TTS at the level of membrane translocation.
Rucks
Elizabeth A
EA
Department of Microbiology, Immunology, and Cell Biology, Robert C. Byrd Health Sciences Center, P.O. Box 9177, West Virginia University, Morgantown, WV 26506-9177, USA.
Olson
Joan C
JC
D036002:ADP Ribose Transferases / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D001427:Bacterial Toxins / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D021381:Protein Transport; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001427:Bacterial Toxins; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; C444820:exoenzyme S; D013755:Tetradecanoylphorbol Acetate