An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces.
Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.
Rodák
L
L
Veterinary Research Institute, Department of Virology, Hudcova 70, 621 32 Brno, Czech Republic. rodak@vri.cz
Valícek
L
L
Smíd
B
B
Nevoránková
Z
Z
D000818:Animals; D019138:Antibodies, Blocking / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D017934:Coronavirus / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005243:Feces / Q000821:virology; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008854:Microscopy, Electron / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology*; D014763:Viral Matrix Proteins / Q000276:immunology
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D014763:Viral Matrix Proteins