Pharmacologically regulated regeneration of functional human pancreatic islets.
Transplantation of allogeneic islets can correct the metabolic abnormalities of Type I diabetes. Limited availability of donor pancreas tissues restricts the application of this therapeutic modality to a subset of eligible recipients. In an attempt to expand the utility of available donor human pancreas tissue, we developed a method to stimulate the proliferation of insulin-secreting beta-cells within human islets. A lentiviral vector was used to introduce into human islets chimeric signaling receptors that are activated to stimulate cell proliferation through interactions with a small-molecule drug called a chemical inducer of dimerization (CID). In vitro exposure of vector-transduced human islets to the CID expanded the number of cells and increased regulated insulin secretion. Transplantation of the regenerated islets into diabetic immunodeficient mice, followed by in vivo administration of the CID, corrected hyperglycemia. This strategy has the potential to reduce the quantity of human islets required for treatment of patients with Type I diabetes.
Kobinger
Gary P
GP
Gene Therapy Program, Division of Medical Genetics, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA 19104, USA.
Deng
Shaoping
S
Louboutin
Jean-Pierre
JP
Vatamaniuk
Marko
M
Rivera
Victor M
VM
Lian
Moh-Moh
MM
Markmann
James F
JF
Clackson
Tim
T
Raper
Steven E
SE
Matschinsky
Franz
F
Wilson
James M
JM
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003920:Diabetes Mellitus / Q000097:blood / Q000473:pathology / Q000628:therapy; D019281:Dimerization; D006801:Humans; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D016381:Islets of Langerhans Transplantation; D051379:Mice; D009924:Organ Culture Techniques; D012038:Regeneration / Q000187:drug effects*; D013997:Time Factors; D014183:Transplantation, Heterologous
D001786:Blood Glucose