title: A simplified spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners.
abstract: A simple, rapid, and sensitive spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners is proposed. This method is based on the reaction of saccharin with tetrachloro-p-benzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in an ethanol:acetone (4:1) medium, producing a violet-red compound (lambda(max) = 550 nm). Beer's law is obeyed in a concentration range of 2.05 x 10(-4) to 3.00 x 10(-3) M with an excellent correlation coefficient (r = 0.9998). The detection limit was 1.55 x 10(-5) M, and the effect of interferences on the spectrophotometric measurements was evaluated. The proposed procedure was applied successfully to the determination of saccharin in noncaloric sweeteners. Recoveries were within 99.2-104.3% with standard deviations ranging from to 0.5-1.6%. Results of the proposed method compare very favorably with those given by the high-performance liquid chromatography method recommended by the Food and Drug Administration.
authors.affiliation: Instituto de Química, UNESP, P.O. Box 355, 14801-970, Araraquara, SP, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Los Weinert
authors.forename: Helena Redigolo
authors.initials: HR
authors.lastname: Pezza
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: de Oliveira
authors.forename: Leonardo
authors.initials: L
authors.lastname: Pezza
mesh_terms: D002703:Chloranil / Q000737:chemistry; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D012439:Saccharin / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013549:Sweetening Agents / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D013549:Sweetening Agents; D002703:Chloranil; D006861:Hydrogen Peroxide; D012439:Saccharin