Synthetic peptides as antigens for antibody production.
The use of synthetic peptide immunogens as a means to generate specific immunological reagents for a variety of purposes has increased markedly in recent years. In this chapter, we outline some of the salient factors to be considered when designing peptide immunogens and describe basic methodologies for the conjugation of short synthetic peptides to immunogenic carrier proteins.
Hancock
David C
DC
Signal Transduction Laboratory, Cancer Research UK, London Research Institute Laboratories, London, UK.
O'Reilly
Nicola J
NJ
D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006433:Hemocyanins / Q000737:chemistry* / Q000276:immunology; D010455:Peptides / Q000737:chemistry* / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D003432:Cross-Linking Reagents; D010455:Peptides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin