The binding of MBL to common bacteria in infectious diseases of children.
OBJECTIVE
To purify Mannan-binding lectin (MBL) from human serum and detect its binding ability to several kinds of bacteria common in infectious diseases of children.


METHODS
MBL was purified from human serum by affinity chromatography on mannan-Sepharose 4B column. Its binding ability to eight species, 97 strains of bacteria was detected by enzyme-linked lectin assay (ELLA).


RESULTS
MBL has different binding ability to bacteria and shows strong binding ability to Klebsiella ornithinolytica and Escherichia coli, but shows relatively lower binding ability to Staphylococcus haemolyticus, Enterobacter cloacae and Staphylococcus epidermidis. To different isolates of Klebsiella pneumoniae, Haemophilus influenzae and Staphylococcus aureus, MBL shows quite different binding ability.


CONCLUSIONS
MBL has different binding ability to different bacteria, and has relatively stronger binding ability to Gram-negative bacteria. Its binding ability to different isolates of certain kinds of bacteria is quite different.
Shang
Shi-qiang
SQ
Affiliated Children's Hospital, Zhejiang University, Hangzhou 310003, China. Shangsq33@mail.hz.zj.cn
Chen
Guo-xian
GX
Shen
Jie
J
Yu
Xiao-hong
XH
Wang
Ke-Yi
KY
D001419:Bacteria / Q000145:classification* / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003141:Communicable Diseases / Q000382:microbiology*; D006801:Humans; D037601:Mannose-Binding Lectin / Q000097:blood / Q000378:metabolism*; D011485:Protein Binding; D013045:Species Specificity
D037601:Mannose-Binding Lectin