title: Concentrations of macrophage inflammatory proteins MIP-1alpha and MIP-1beta and interleukin 8 (il-8) in lyme borreliosis.
abstract: BACKGROUND
  Components of the spirochete Borrelia burgdorferi sensu lato ( B. burgdorferi s.l.) do not have chemotactic activity. However, B. burgdorferi s.l. causes a chemotactic response, probably by stimulating synthesis of cytokines of the chemokine family by host cells. Our aim was to confirm that the synthesis of chemokines is increased in Lyme borreliosis and that they may account for leukocyte migration, thus being involved in inflammatory response.
  
  
  METHODS
  We measured concentrations of chemokines: interleukin 8 (Il-8) and macrophage inflammatory protein 1alpha and 1beta (MIP-1alpha, -1beta) in serum of 20 patients with erythema migrans (early localized infection, group I), of 19 patients with Lyme arthritis (chronic infection, group II), and in serum and cerebrospinal fluid (CSF) of 20 patients with neuroborreliosis (early disseminated infection, group III), before and after 2 weeks of antibiotic therapy (examinations 1 and 2), as well as in the sera of 12 healthy blood donors and CSF of ten patients in whom Lyme borreliosis and meningitis were excluded (control group). Interleukin 1beta (Il-1beta) level in serum and CSF and pleocytosis of CSF were assessed simultaneously.
  
  
  RESULTS
  The mean concentrations of all studied chemokines in serum were significantly elevated in all study groups in examination 1 and decreased in examination 2. The concentration of Il-8 in serum was higher in group I and the concentration of MIP-1alpha in group III was higher in comparison with group II. Serum concentrations of all chemokines in group I and III correlated with the concentration of Il-1beta, while in group II this correlation appeared only for Il-8 in examination 2. Concentrations of all chemokines in CSF were significantly increased, but as for MIP-1alpha and 1beta they remained lower than in serum. The concentration of Il-8 in CSF was variable and reached values several fold higher than in the serum in some patients. There was no correlation between chemokine concentrations and CSF pleocytosis.
  
  
  CONCLUSIONS
  The synthesis of chemokines (Il-8, MIP-1alpha and 1beta) is increased in Lyme borreliosis and, at least in the early stages of the disease, is related to the synthesis of Il-1beta. Chemokine concentrations depend on the clinical form of Lyme borreliosis, with a tendency for higher values in early infection (erythema migrans and neuroborreliosis). Of the chemokines studied, Il-8 created a chemotactic gradient towards the inflammation site, and thus might be responsible for leukocyte migration.
authors.affiliation: Dept. of Infectious Diseases and Neuroinfections, Medical University of Białystok, ul. Zurawia 14, P-15-540 Białystok, Poland. neuroin@amb.edu.pl
authors.forename: S
authors.initials: S
authors.lastname: Grygorczuk
authors.forename: S
authors.initials: S
authors.lastname: Pancewicz
authors.forename: J
authors.initials: J
authors.lastname: Zajkowska
authors.forename: M
authors.initials: M
authors.lastname: Kondrusik
authors.forename: R
authors.initials: R
authors.lastname: Rwierzbińska
authors.forename: T
authors.initials: T
authors.lastname: Hermanowska-Szpakowicz
mesh_terms: D000328:Adult; D000368:Aged; D001782:Blood Donors; D016022:Case-Control Studies; D002465:Cell Movement; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D002633:Chemotaxis; D018450:Disease Progression; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007249:Inflammation; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid; D007962:Leukocytes / Q000276:immunology / Q000502:physiology; D008193:Lyme Disease / Q000276:immunology*; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged
chemical_list: D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins