[Inhibitory effect of trisodium phosphonoformate (PFA) on duck hepatitis B virus (DHBV) DNA in vivo].
This paper described that DHBV infected ducklings were given with PFA 250 mg, 125 mg, 62.5 mg/kg b. i. d for 21 days intraperitioneally (i.p.) and dynamics of duck hepatitis B virus DNA (DHBV DNA) and duck hepatitis B surface antigen (DHBsAg) were detected in serum and distribution of DHBV DNA was tested in liver, kidney, pancreas and spleen. Supercoiled DNA (SC DNA) extracted from liver was analysed with Southern blot. The results showed that PFA in a dose of 125 mg and 250 mg/kg inhibited significantly the serum DHBsAg; doses of 125 mg and 250 mg/kg inhibited the serum DHBV DNA from 14 to 21 days, and dose of 250 mg/kg inhibited the serum DHBV DNA earlier even at 7 days after being given with PFA. Doses of 125 mg and 250 mg/kg inhibited obviously DHBV DNA in liver and kidney, but no inhibition was found in spleen and pancreas. The replication of DHBV DNA in liver of ducklings treated with PFA in a dose of 250 mg/kg for 21 days showed that the PFA inhibited significantly the synthesis of linear DNA (L DNA), relaxed circular DNA (RC DNA) and SC DNA. But DHBsAg and DHBV DNA in serum and DHBV DNA in liver, kidney, pancreas and spleen after treated with PFA for 21 days were still present, this meant that PFA treatment didn't clear off the virus genome in infected ducklings, the "rebounding" of DHBV appeared after cessation of the PFA treatment.
Chen
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Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050.
Chen
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D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D004261:DNA Replication / Q000187:drug effects; D015536:Down-Regulation; D004372:Ducks; D017245:Foscarnet / Q000008:administration & dosage*; D018347:Hepadnaviridae Infections / Q000188:drug therapy* / Q000662:veterinary* / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000235:genetics; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy* / Q000821:virology; D007668:Kidney / Q000187:drug effects / Q000821:virology; D008099:Liver / Q000187:drug effects / Q000821:virology; D010179:Pancreas / Q000187:drug effects / Q000821:virology; D013154:Spleen / Q000187:drug effects / Q000821:virology
D000998:Antiviral Agents; D017245:Foscarnet