A comparative analysis of the composition and organization of two subtelomeric repeat families in Aegilops speltoides Tausch. and related species.
The structural organization and evolution of two tandemly repeated families, Spelt1 and Spelt52, located in the subtelomeric regions of Aegilops speltoides chromosomes were studied. The Spelt1 family of sequences with a monomer length of 178 bp was characterized by cloning and sequence analysis of polymerase chain reaction (PCR) products. Members of the Spelt1 family revealed sequence similarities exceeding 95%. This conservation has remained despite divergence of species in Aegilops section Sitopsis and after independent multiple amplification events in the genome of Ae. speltoides. Sequences representing the Spelt52 family were cloned, sequenced and compared with other sequences in databases. The Spelt52 repeat family contains monomers of two types, Spelt52.1 and Spelt52.2. The two monomers share a homologous stretch of 280 bp and have two regions without sequence similarity of 96 bp and 110 bp, respectively. PCR analysis was conducted to 15 lines in Ae. speltoides Tausch., Ae. longissima Schw. & Mushc., Ae. sharonensis Eig., Ae. bicornis (Forssk) Jaub.&Sp., and Ae. searsii Feld.&Kis. using primers to the homologous and nonhomologous regions of Spelt52 family. Intraspecies and interspecies differences in the occurrence and abundance of combinations of Spelt52.1 and Spelt52.2 monomers were detected. The use of primers to telomeric and subtelomeric repeats followed by Southern hybridization, cloning, and sequence analysis demonstrated that Spelt1 and Spelt52 are localized close to each other and to telomeric repeats. The efficiency of a PCR approach for the analysis of telomeric/subtelomeric junction regions of chromosomes is discussed.
Salina
E A
EA
Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk 630090, Russia. salina@bionet.nsc.ru
Adonina
I G
IG
Vatolina
T Yu
TY
Kurata
N
N
D018745:Genome, Plant; D006109:Poaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences