cDNA microarray gene expression analysis and toxicological phenotype for anticancer drug.
Toxicogenomics, the subdiscipline that merges genomics with toxicology, hold the promise to contributing toward the goal of elucidating mechanism by studying genomic profiling related with various drugs. The application of gene expression profiling technology to examine multiple genes and signaling pathways promises a significant advance in understanding the toxic mechanisms of various drugs and prediction of new drug candidate. Toxicogenomics is emerging field combining genomics and bioinformatics to identify and characterize mechanisms of toxicity of drug and various compounds. The principal hypothesis underlying on this field is that chemical-specific pattern of altered gene expression is related with each chemicals properties, especially toxicological property, and it will be revealed using high-density microarray analysis of sample from exposed organisms. So, in this study we compare the gene expression pattern of two anticancer drugs paclitaxel and orally absorbable paclitaxel, using the cDNA microarray. And from the result of this study, it is possible to provide the new possibility for genome-wide insight into mechanism of their anticancer activity and toxicological phenotype.
Lee
Gyoung-Jae
GJ
Research Institute, Shin-Won Scientific Co., Ltd. Gyeonggi-do, South Korea.
Lee
Wan-Seon
WS
Jeon
Ki-Seon
KS
Um
Chan-Hwi
CH
Kim
Yang-Suk
YS
Kim
Seung-Jun
SJ
Lee
Chang-Hyeon
CH
Yoon
Hyun-Kyu
HK
Hwang
Seung Yong
SY
Park
Joon-Suk
JS
Hwang
Jae-Woong
JW
Kang
Kyung-Sun
KS
Lee
Yong-Soon
YS
Kim
Maeng-Sup
MS
Chon
Kum-Jin
KJ
Yeo
Chan-Dong
CD
Kang
Jong-Soo
JS
D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017239:Paclitaxel / Q000008:administration & dosage / Q000633:toxicity*; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D012737:Sex Factors; D043922:Toxicogenetics
D000970:Antineoplastic Agents; D017239:Paclitaxel