Expression of cutaneous lymphocyte-associated antigen and E-selectin ligand by circulating human memory CD4+ T lymphocytes specific for herpes simplex virus type 2.
Virus-specific memory T lymphocytes traffic to sites of viral infection. Herpes simplex virus (HSV) type 2-specific CD4(+) and CD8(+) T lymphocytes differ with regard to their homing kinetics to infected tissues. We studied the expression of cutaneous lymphocyte-associated antigen (CLA) and E-selectin ligand (ESL) by HSV-2-specific CD4(+) T lymphocytes. Virus-reactive T lymphocytes were identified ex vivo by CD154 or interferon-gamma up-regulation. We detected selective expression of CLA by HSV-2-reactive CD4(+) T lymphocytes, but at levels lower than those we previously observed for CD8(+) T lymphocytes. Short-term HSV-2-reactive CD4(+) lines generated from peripheral-blood mononuclear cells preferentially express CLA, compared with cytomegalovirus- or influenza-specific cells. CLA is expressed by HSV-2-reactive cells that are initially CLA negative before restimulation. Short-term culture-expanded HSV-2-specific CD4(+) T lymphocytes also selectively express ESL. These findings have implications for the optimization of vaccines for HSV and other cutaneous pathogens.
González
Julio C
JC
Department of Medicine, University of Washington, Seattle, Washington, USA.
Kwok
William W
WW
Wald
Anna
A
McClurkan
Christopher L
CL
Huang
Jay
J
Koelle
David M
DM
D015496:CD4-Positive T-Lymphocytes / Q000145:classification / Q000276:immunology*; D019040:E-Selectin / Q000378:metabolism*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D008024:Ligands; D008213:Lymphocyte Activation; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*; D012867:Skin / Q000276:immunology / Q000821:virology
D019040:E-Selectin; D008024:Ligands; D016201:Receptors, Lymphocyte Homing