Optical sensor for the determination of glucose based on KIO4 chemiluminescence detection.
A method to determine glucose using an optical sensor prepared by entrapping glucose oxidase into silica sol-gel column has been developed. The silica sol-gel film was coated on alumina substrate. The optical sensor is based on the chemiluminescence intensity from the reaction of periodate and hydrogen peroxide in K2CO3 medium. The effect of the ratio of water and alcohol for the preparation of TEOS sol on chemiluminescence intensity was investigated. The effects of pH of enzyme reactor, concentrations of potassium periodate and SDS, and flow rate on the chemiluminescence intensity were studied to find the optimum experimental conditions to determine glucose. The chemiluminescence intensity increased linearly with increasing glucose concentration from 5.0 x 10(-4) M to 1.0 x 10(-7) M and the detection limit was 4.0 x 10(-8) M. Interference effects from some metal ions on chemiluminescence intensity were also investigated.
Li
Ming
M
Department of Chemistry, Kyungpook National University, Taegu 702 701, Korea.
Lee
Sang Hak
SH
Bae
Zun Ung
ZU
Lee
Kwang Pill
KP
Park
Yu Chul
YC
Lee
Mu Sang
MS
D000537:Aluminum Oxide / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002412:Cations / Q000737:chemistry; D004795:Enzyme Stability; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008163:Luminescent Measurements; D010504:Periodic Acid / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide / Q000138:chemical synthesis; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D002412:Cations; D017680:Potassium Compounds; D012821:Silanes; D010504:Periodic Acid; D012967:Sodium Dodecyl Sulfate; C040733:tetraethoxysilane; D058428:Silica Gel; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D005947:Glucose; D000537:Aluminum Oxide; C037192:potassium periodate