Mitochondrial targeting sequence of the influenza A virus PB1-F2 protein and its function in mitochondria.
The influenza A virus PB1-F2 protein predominantly localizes in the mitochondria of virus-infected cells. A series of cDNAs encoding N- and C-terminal deletion mutants and site-directed mutagenesis of the basic residues of PB1-F2 appended to 3xFLAG revealed the domain from residues 46 to 75 to be both necessary and sufficient for mitochondrial targeting. In addition, the subdomain residues 63-75 and both Lys73 and Arg75 are minimally required for mitochondrial localization. Transfection of untagged- and 3xFLAG tagged-PB1-F2 into Vero, HeLa and MDCK cells changed the mitochondrial morphology from a filamentous to a dotted structure and suppressed the inner-membrane potential.
Yamada
Hiroshi
H
Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Kuramoto-cho 3-18-15, 770-8503, Japan.
Chounan
Ritsu
R
Higashi
Youichirou
Y
Kurihara
Naoki
N
Kido
Hiroshi
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001120:Arginine / Q000737:chemistry; D001562:Benzimidazoles; D002460:Cell Line; D004285:Dogs; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D008239:Lysine / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology
D000911:Antibodies, Monoclonal; D001562:Benzimidazoles; D005456:Fluorescent Dyes; C442816:PB1-F2 protein, Influenza A virus; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D001120:Arginine; D016650:Fluorescein-5-isothiocyanate; D008239:Lysine; C017807:bisbenzimide ethoxide trihydrochloride