Microstructured arrays with pre-synthesized capture probes for DNA detection based on metal nanoparticles and silver enhancement.
DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal.
Zhang
Guo-Jun
GJ
Institute for Physical High Technology, PO Box 100239, 07702 Jena, Germany.
Möller
Robert
R
Kretschmer
Robert
R
Csáki
Andrea
A
Fritzsche
Wolfgang
W
D004247:DNA / Q000032:analysis / Q000737:chemistry; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D012834:Silver / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
D015342:DNA Probes; D012834:Silver; D006046:Gold; D004247:DNA