Genes involved in aniline degradation by Delftia acidovorans strain 7N and its distribution in the natural environment.
Aniline-degraders were isolated from activated sludge and environmental samples and classified into eight phylogenetic groups. Seven groups were classified into Gram-negative bacteria, such as Acidovorax sp., Acinetobacter sp., Delftia sp., Comamonas sp., and Pseudomonas sp., suggesting the possible dominance of Gram-negative aniline-degraders in the environment. Aniline degradative genes were cloned from D. acidovorans strain 7N, and the nucleotide sequence of the 8,039-bp fragment containing eight open reading frames was determined. Their deduced amino acid sequences showed homologies to glutamine synthetase (GS)-like protein, glutamine amidotransferase (GA)-like protein, large and small subunits of aniline dioxygenase, reductase, LysR-type regulator, small ferredoxin-like protein, and catechol 2,3-dioxygenase, suggesting a high similarity of this gene cluster to those in P. putida strain UCC22 and Acinetobacter sp. strain YAA. Polymerase chain reaction (PCR) and sequencing analyses of GS-like protein gene segments of other Gram-negative bacteria suggested that Gram-negative bacteria have aniline degradative gene that can be divided into two distinctive groups.
Urata
Masaaki
M
Biotechnology Research Center, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Uchida
Eiji
E
Nojiri
Hideaki
H
Omori
Toshio
T
Obo
Rie
R
Miyaura
Noriko
N
Ouchiyama
Naoki
N
D000814:Aniline Compounds / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D020605:Delftia acidovorans / Q000235:genetics* / Q000378:metabolism; D017753:Ecosystem; D016680:Genome, Bacterial; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification; D005810:Multigene Family; D016366:Open Reading Frames; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000814:Aniline Compounds; C023650:aniline