title: Production and characterization of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of Japanese bunching onion (A. fistulosum L.).
abstract: First and second backcrosses of amphidiploid hybrids (2n = 4x = 32, genomes AAFF) between shallot (Allium cepa Aggregatum group) and A. fistulosum were conducted to produce A. cepa - A. fistulosum alien addition lines. When shallot (A. cepa Aggregatum group) was used as a pollinator, the amphidiploids and allotriploids set germinable BC(1) and BC(2) seeds, respectively. The 237 BC(1) plants mainly consisted of 170 allotriploids (2n = 3x = 24, AAF) and 42 hypo-allotriploids possessing 23 chromosomes, i.e., single-alien deletions (2n = 3x-1 = 23, AAF-nF). The single-alien deletions in the BC(1) progeny showed dwarfing characteristics and were discriminated from the allotriploids (2n = 24) and hyper-allotriploids (2n = 25) by means of flow cytometric analysis. The chromosome numbers of 46 BC(2) seedlings varied from 16 to 24. Eight monosomic additions (2n = 2x+1 = 17, AA+nF) and 20 single-alien deletions were found in these BC(2) seedlings. Consequently, six kinds of A. cepa - A. fistulosum alien chromosome additions possessing different chromosome numbers (2n = 17, 18, 20, 21, 22, 23) were recognized in the BC(1) and BC(2) populations. A total of 79 aneuploids, including 62 single-alien deletions, were analyzed by a chromosome 6F-specific isozyme marker (Got-2) in order to recognize its existence in their chromosome complements. This analysis revealed that two out of 62 single-alien deletions did not possess 6F. One (AAF-6F) out of the possible eight single-alien deletions could be identified at first. The present study is a first step toward the development of a useful tool, such as a complete set of eight different single-alien deletions, for the rapid chromosomal assignment of genes and genetic markers in A. fistulosum.
authors.affiliation: The United Graduated School of Agricultural Sciences, Tottori University, Tottori 680-8553, Japan.
authors.forename: Tran Thi Minh
authors.initials: TT
authors.lastname: Hang
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shigyo
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamauchi
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Tashiro
mesh_terms: D000490:Allium / Q000201:enzymology / Q000235:genetics*; D000782:Aneuploidy; D024442:Aspartate Aminotransferase, Mitochondrial / Q000032:analysis; D032461:Chromosomes, Plant / Q000502:physiology*; D003433:Crosses, Genetic; D005434:Flow Cytometry; D007527:Isoenzymes / Q000032:analysis; D019697:Onions / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D024442:Aspartate Aminotransferase, Mitochondrial