Ini1/hSNF5 is dispensable for retrovirus-induced cytoplasmic accumulation of PML and does not interfere with integration.
Retroviral infection triggers the cytoplasmic translocation of two Crm1-dependent shuttle factors, namely the Ini1 (integrase interactor 1, hSNF5) and the promyelocytic leukemia (PML) protein. Blocking nuclear export of shuttle factors by leptomycin B increases the efficiency of retroviral integration, suggesting that some may mediate antiviral activity. While PML was shown to counteract proviral establishment, it remained unclear whether Ini1, a protein implicated in various processes during human immunodeficiency virus replication, has the same potential. Employing RNA interference-mediated knock-down of Ini1, we show here that the simultaneous accumulation of both proteins in the cytoplasm likely reflects two non-interdependent phenomena. Furthermore, Ini1 does not interfere with retroviral integration, as cells lacking Ini1 show no increased infection susceptibility.
Boese
Annette
A
Unité de Biologie Cellulaire du Noyau, Institut Pasteur, 28 rue du Dr Roux, Paris, France. boese@pasteur.fr
Sommer
Peter
P
Gaussin
Armelle
A
Reimann
Andreas
A
Nehrbass
Ulf
U
D017304:Annexin A5 / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D015153:Blotting, Western; D002458:Cell Fractionation; D002868:Chromosomal Proteins, Non-Histone; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000187:drug effects; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000187:drug effects; D016133:Polymerase Chain Reaction; D000071156:Promyelocytic Leukemia Protein; D034622:RNA Interference; D012192:Retroviridae Infections / Q000201:enzymology* / Q000378:metabolism; D000071796:SMARCB1 Protein; D014157:Transcription Factors / Q000096:biosynthesis* / Q000187:drug effects; D025521:Tumor Suppressor Proteins; D016662:Virus Integration / Q000502:physiology*
D017304:Annexin A5; D000903:Antibiotics, Antineoplastic; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005231:Fatty Acids, Unsaturated; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human; C038753:leptomycin B