title: Quantitative determination of amygdalin epimers from armeniacae semen by liquid chromatography.
abstract: D-amygdalin and its conversion product, neoamygdalin, were quantitatively analyzed on reverse-phase, high-performance liquid chromatography with an optimized eluent of 10 mM sodium phosphate buffer (pH 3.1) containing 8.5% acetonitrile. Linearity between concentrations and detector responses was obtained in the range from 0.05 to 0.5 mM. The detection limits for D-amygdalin and neoamygdalin were approximately 5 microM per injected amount. Armeniacae semen contains not only amygdalin but also emlusin, which is an enzyme that hydrolyzes amygdalin. When extracting amygdalin from a whole piece of armeniacae semen in boiling water, there was almost no influence of emulsin; which increased the extraction efficiency. However, conversion of d-amygdalin into neoamygdalin at high temperature was found. In this report, we solved this problem by using 4% citric acid as an extractant. This solution also prevented the extraction process from being affected by emulsin. In addition, the extraction efficiency remained the same as that when methanol was used as an extractant, regardless of the cutting size.
authors.affiliation: Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Dongdaemoon-gu Hoegi-dong, Seoul 130-701, Korea.
authors.forename: Ja-Yong
authors.initials: JY
authors.lastname: Koo
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Hwang
authors.forename: Sonhae
authors.initials: S
authors.lastname: Cho
authors.forename: Je-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Seon-Pyo
authors.initials: SP
authors.lastname: Hong
mesh_terms: D000678:Amygdalin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027861:Prunus / Q000196:embryology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000678:Amygdalin