Genetic determinant of intrinsic quinolone resistance in Fusobacterium canifelinum.
Fourteen fluoroquinolone-resistant fusobacterial strains, originating from cats or dogs, were characterized by sequencing of the 16S-23S and 16S rRNA genes and DNA-DNA hybridization and were described as a new species, Fusobacterium canifelinum. All of the strains are intrinsically resistant (MIC, >4 g/ml) to levofloxacin and other fluoroquinolones. Compared to the quinolone resistance-determining region (gyrA) of the susceptible relative F. nucleatum, we found that Ser79 was replaced with leucine and Gly83 was replaced with arginine.
Conrads
Georg
G
Division of Oral Microbiology and Immunology University Hospital, Pauwelsstrasse 30, D-52057 Aachen, Germany. gconrads@ukaachen.de
Citron
Diane M
DM
Goldstein
Ellie J C
EJ
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics*; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology*; D005673:Fusobacterium / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D005674:Fusobacterium Infections / Q000382:microbiology / Q000662:veterinary*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027081:DNA Gyrase