title: Cytomegalovirus (CMV) glycoprotein B genotype and CMV DNA load in the amniotic fluid of infected fetuses.
abstract: OBJECTIVE
  To assess the value of both cytomegalovirus (CMV) DNA quantification in amniotic fluid (AF) and CMV glycoprotein B (gB) genotype as prognostic factors in CMV congenital infection.
  
  
  METHODS
  Quantification of CMV DNA was performed prospectively by real-time PCR and gB genotypes were analysed by gene sequencing analysis in the amniotic fluid of 42 fetuses infected by CMV. These were correlated with clinical data on fetal anomalies and outcome.
  
  
  RESULTS
  The proportion of severely symptomatic fetuses was similar in each gB genotype group. Median CMV DNA load was higher in the group of symptomatic fetuses but this difference was not significant and high and low viral loads were found in both groups.
  
  
  CONCLUSIONS
  Neither gB genotype nor CMV DNA load in AF correlate with the severity of CMV congenital infection and these markers are unlikely to prove useful for the management of congenital infection.
authors.affiliation: Service de Gynécologie Obstétrique, Hôpital de Poissy-St-Germain, 10 rue du Champ Gaillard, 78300 Poissy, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Picone
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Costa
authors.forename: Marianne
authors.initials: M
authors.lastname: Leruez-Ville
authors.forename: Pauline
authors.initials: P
authors.lastname: Ernault
authors.forename: Martine
authors.initials: M
authors.lastname: Olivi
authors.forename: Yves
authors.initials: Y
authors.lastname: Ville
mesh_terms: D000653:Amniotic Fluid / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000000981:diagnostic imaging / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005315:Fetal Diseases / Q000821:virology*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011379:Prognosis; D014463:Ultrasonography; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus