A micro-chemiluminescence determination of cyanide in whole blood.
A reactant volume self-controlled micro-device was presented and applied to the flow injection chemiluminescence (CL) for determination of cyanide in whole blood. A mini distiller was fabricated for cyanide extraction from the blood samples with the extraction efficiencies of cyanide > or = 98%. A fluidic control platform with air driving was fabricated. The described system showed the features of easy fabrication, undiluted sample injection, safe analysis operation, and suitability for automatic cyanide analysis. The calibration curve showed linearity in the cyanide concentration range of 5.0 x 10(-7) to 5.0 x 10(-5) mol l(-1) with the detection limits (3sigma) of 2.3 x 10(-7) mol l(-1). CL peak-height precision was 1.9% R.S.D. (n = 11) at the 1.0 x 10(-6) mol l(-1) cyanide level. The new devices were applied to the analysis of cyanide in rabbit whole blood samples and the results agreed well with those obtained from official method.
Lv
Jiagen
J
College of Chemistry and Material Science, Shaanxi Normal University, Xian 710062, China. lvjiagen413@yahoo.com.cn
Zhang
Zhujun
Z
Li
Jindong
J
Luo
Lirong
L
D000818:Animals; D003486:Cyanides / Q000097:blood*; D017022:Flow Injection Analysis; D005554:Forensic Medicine / Q000295:instrumentation / Q000379:methods*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D011042:Poisons / Q000097:blood*; D011817:Rabbits; D012972:Sodium Hydroxide
D003486:Cyanides; D007202:Indicators and Reagents; D011042:Poisons; D012972:Sodium Hydroxide