New constituents from Crinum latifolium with inhibitory effects against tube-like formation of human umbilical venous endothelial cells.
Six compounds (1-6) were isolated from the methanol extract of Crinum latifolium by bioassay-guided separation. Among the six isolates, compounds 2 and 6 were new metabolites. Their structures were established as 4-senecioyloxymethyl-3,4-dimethoxycoumarin (2) and 5,6,3'-trihydroxy-7,8,4'-trimethoxyflavone (6) based on spectroscopic analyses. Compound 2 was found to be strongly inhibitory against the in vitro tube-like formation of human umbilical venous endothelial cells (HUVECs) while manifesting no cytotoxicity in tumor cell lines (B16F10, HCT116). Significant inhibitory activity (inhibition percentage, 53.5%) was still observed at concentrations as low as 1 microg/mL. Compound 6 showed a modest inhibitory effect on the tube-like formation of HUVECs. Other compounds, including cycloartenol (1), 4',7-dihydroxy-3'-methoxyflavan (3), 4',7-dihydroxyflavan (4), and 2',4',7-trihydroxydihydrochalcone (5) were found to be nearly inactive.
Nam
Nguyen-Hai
NH
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea. nhnam@hotmail.com
Kim
Yong
Y
You
Young-Jae
YJ
Hong
Dong-Ho
DH
Kim
Hwan-Mook
HM
Ahn
Byung-Zun
BZ
D020533:Angiogenesis Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor / Q000187:drug effects; D003374:Coumarins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D031407:Crinum; D004730:Endothelium, Vascular / Q000187:drug effects; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D014472:Umbilicus
D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts