Transactivation of peroxisome proliferator-activated receptor alpha by green tea extracts.
Tea is a popular beverage. Recently, green tea was reported to increase the number of peroxisomes in rats. In this study, to find out whether the green tea-induced proliferation of peroxisomes is mediated by PPARalpha , a transient transfection assay was carried out to investigate the interactions of tea extracts (green tea, black tea,oolong tea and doongule tea) and tea components (epigallocatechin gallate, epigallocatechin, epicatechin gallate, epicatechin and gallic acid), with mouse cloned PPARalpha . Green tea and black tea extracts, and epigallocatechin gallate, a major component of fresh green tea leaves, increased the activation of PPAalpha 1.5-2 times compared with the control. It is suggested that the green tea induced-peroxisomal proliferation may be mediated through the transactivation of PPARalpha and that epigallocatechin gallate may be an effective component of green tea leaves. This would account for the increase in the number of peroxisomes and the activity of peroxisomal enzymes previously reported. However, black tea, a fully fermented product, had a stronger effect than oolong tea extract. These results also suggest, that in addition to epigallocatechin gallate, green tea leaves may possess some active chemicals newly produced as a result of the fermentation process, which act on PPARalpha like other peroxisome proliferators.
Lee
Kookkyung
K
Department of Veterinary Medicine, Cheju National University, Jeju 690-756, Korea. syeon@cheju.ac.kr
D000818:Animals; D019556:COS Cells / Q000201:enzymology; D028241:Camellia sinensis; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002522:Chlorocebus aethiops; D047493:PPAR alpha / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D010957:Plasmids; D013662:Tea; D015533:Transcriptional Activation / Q000187:drug effects; D014162:Transfection / Q000662:veterinary
D047493:PPAR alpha; D010936:Plant Extracts; D013662:Tea; C465546:green tea extract AR25; D002392:Catechin; C045651:epigallocatechin gallate