title: In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings.
abstract: An in-house mycobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.
authors.affiliation: INEI-ANLIS "Carlos G. Malbrán," Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.
authors.forename: Norberto
authors.initials: N
authors.lastname: Símboli
authors.forename: Howard
authors.initials: H
authors.lastname: Takiff
authors.forename: Ruth
authors.initials: R
authors.lastname: McNerney
authors.forename: Beatriz
authors.initials: B
authors.lastname: López
authors.forename: Anandi
authors.initials: A
authors.lastname: Martin
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Palomino
authors.forename: Lucía
authors.initials: L
authors.lastname: Barrera
authors.forename: Viviana
authors.initials: V
authors.lastname: Ritacco
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009160:Mycobacteriophages / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development / Q000821:virology; D011933:Reagent Kits, Diagnostic; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
chemical_list: D000904:Antibiotics, Antitubercular; D011933:Reagent Kits, Diagnostic; D012293:Rifampin