Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS'::gfp reporter plasmid.
We constructed a reporter system to detect a superoxide-generating methyl viologen using SoxRS of Escherichia coli and GFP of Aequorea victoria. E. coli carrying this plasmid exhibited strong fluorescence when grown in the presence of a superoxide-generating reagent methyl viologen. The fluorescence intensity observed in the stationary phase culture of the transformant increased in response to the methyl viologen concentration in a range of 0.01 microM to 10 microM.
Shibuya
Akihito
A
Department of Biological Information, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Tsukagoshi
Norihiko
N
Ohtsu
Iwao
I
Dokyu
Noriyuki
N
Aono
Rikizo
R
D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D005453:Fluorescence; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D007202:Indicators and Reagents; D008722:Methods; D010269:Paraquat / Q000032:analysis*; D013481:Superoxides / Q000737:chemistry*; D015534:Trans-Activators / Q000235:genetics; D014161:Transduction, Genetic
D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; D015534:Trans-Activators; D013481:Superoxides; C070725:SoxS protein, E coli; D049452:Green Fluorescent Proteins; D010269:Paraquat