N-terminal sequence of a core protein from a biglycan isolated from bovine aorta.
A biglycan was isolated from bovine aorta intima media by 4M guanidine HCl extraction of the tissue; the material was fractionated and purified by using isopycnic ultracentrifugation and DEAE Sephacel ion-exchange chromatography. Core proteins, resulting from digestion of the proteoglycan preparation with chondroitinase ABC, were resolved by SDS-polyacrylamide gel electrophoresis into three bands. The apparent molecular weight of the fast migrating major protein band was 47 kDa and the other slow-moving minor protein bands were 90 and 105 kDa. These proteins were recognized by a monoclonal anti-proteoglycan deltaDi-6S (MAb 3-B-3/Cl). The amino acid composition of 47 kDa core protein revealed a high content of aspartic acid, glutamic acid and leucine, similar to those found for biglycans isolated from bovine cartilage, rat vascular smooth muscle cell culture and human bone. The N-terminal sequence of 47 kDa core protein was determined as Asp-Glu-Glu-Ala-X-Gly-Ala-Glu-Thr-Thr-X-Gly-Ile-Pro-Asp which is identical to the sequence of bovine articular cartilage biglycan. The proteoglycan had two glycosaminoglycan chains.
Zhu
X L
XL
Departments of Applied Health Sciences, Tulane University Medical Center, New Orleans, Louisiana, USA.
Radhakrishnamurthy
B
B
Xu
J H
JH
Srinivasan
S R
SR
Berenson
G S
GS
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity / Q000276:immunology; D001011:Aorta / Q000737:chemistry* / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D058578:Biglycan; D002358:Cartilage, Articular / Q000737:chemistry; D002417:Cattle; D019765:Chondroitin ABC Lyase; D003238:Connective Tissue / Q000737:chemistry* / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D032389:Myocytes, Smooth Muscle / Q000737:chemistry; D017434:Protein Structure, Tertiary / Q000502:physiology; D011509:Proteoglycans / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000911:Antibodies, Monoclonal; C546884:BGN protein, human; C546886:Bgn protein, rat; D058578:Biglycan; D006025:Glycosaminoglycans; D009124:Muscle Proteins; D011509:Proteoglycans; D001224:Aspartic Acid; D018698:Glutamic Acid; D019765:Chondroitin ABC Lyase; D007930:Leucine