Molecular and genetic organization of Drosophila melanogaster polytene chromosomes: evidence for two types of interband regions.
The 3A and 60E regions of Drosophila melanogaster polytene chromosomes containing inserted copies of the P[1ArB] transposon have been subjected to an electron microscopic (EM) analysis. We show that both inserts led to formation of new bands within the interband regions 3A4/A6 and 60E8-9/E10. This allowed us to clone DNA of these interbands. Their sequences, as well as those of DNA from other four interbands described earlier, have been analyzed. We have found that, with the exception of 60E8-9/E10 interband, all other five regions under study corresponded to 5' or 3' ends of genes. We have further obtained the evidence for 60E8-9/E10 interband to harbor the 'housekeeping' RpL19 gene, which is transcribed in many tissues, including salivary glands. Based upon the genetic heterogeneity of the interbands observed a revised model of polytene chromosome organization is discussed.
Demakov
Sergei
S
Institute of Cytology and Genetics, 630090 Novosibirsk, Russia.
Gortchakov
Andrei
A
Schwartz
Yuri
Y
Semeshin
Valery
V
Campuzano
Sonsoles
S
Modolell
Juan
J
Zhimulev
Igor
I
D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D008854:Microscopy, Electron; D017422:Sequence Analysis, DNA
D004251:DNA Transposable Elements