title: Laser-excited time-resolved Shpol'skii spectroscopy for the direct analysis of dibenzopyrene isomers in liquid chromatography fractions.
abstract: We present a unique method for the unambiguous determination of dibenzo[a,l]pyrene, dibenzo[a,e]pyrene, dibenzo[a,h]pyrene, dibenzo[a,i]pyrene, and dibenzo[e,l]pyrene in high-performance liquid chromatography (HPLC) fractions. Chemical analysis is performed via laser-excited time-resolved Shpol'skii spectroscopy with the aid of a cryogenic fiber-optic probe, pulsed tunable dye laser, spectrograph, and intensified charge-coupled device. Unambiguous identification is accomplished via wavelength time matrix formats, which give simultaneous access to spectral and lifetime information. Prior to spectroscopic analysis, HPLC fractions are pre-treated with liquid-liquid extraction or solid-liquid extraction at the tip of the fiber-optic probe. Solid-liquid extraction gives the best limits of detection, which vary from 40 pg mL(-1) (dibenzo[a,l]pyrene) to 0.2 ng mL(-1)(dibenzo[e,l]pyrene).
authors.affiliation: Department of Chemistry, P. O. Box 25000, University of Central Florida, Orlando, Florida 32816-2366, USA.
authors.forename: Shenjiang
authors.initials: S
authors.lastname: Yu
authors.forename: Andres D
authors.initials: AD
authors.lastname: Campiglia
mesh_terms: D001580:Benzopyrenes / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003080:Cold Temperature; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007536:Isomerism; D007834:Lasers; D055100:Optical Fibers; D013048:Specimen Handling / Q000379:methods; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D001580:Benzopyrenes; D004785:Environmental Pollutants