Development of a novel compact sonicator for cell disruption.
Ultrasound microbial cell disrupters operating at around 20 kHz are often physically large and, due to significant heating, can be unsuitable for small sample volumes where biochemical integrity of the extracted product is required. Development of a compact device based on a 63.5-mm diameter, 6.5-mm thick tubular transducer for rapid cell disruption in small-volume samples in a high-intensity acoustic cavitation field with minimal temperature rises is described here. Suspensions of Saccharomyces cerevisiae were exposed to cavitation for various times in the compact device and a 20-kHz probe sonicator. Cell disruption was assessed by protein release and by staining. Yeast cell disruption was greater in the novel 267-kHz sonicator than in the 20-kHz probe sonicator for the same exposure time. A 1-dimensional (1-D) transfer matrix model analysis for piezoelectric resonators was applied to an axial cross-section of the tubular sonicator to predict frequencies of mechanical resonance in the sample volume associated with maximum acoustic pressure. Admittance measurements identified frequencies of electrical resonance. Ultrasonic cavitation noise peaks were detected by a hydrophone at both the mechanical and electrical resonances. Cell breakage efficiency was twice as great in terms of protein released per dissipated watt at the mechanical resonance predicted by the model, compared to those at the electrical resonance frequencies. The results form a basis for rational design of an ultrasound cell disruption technique for small-volume samples.
Borthwick
K A J
KA
Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, UK.
Coakley
W T
WT
McDonnell
M B
MB
Nowotny
H
H
Benes
E
E
Gröschl
M
M
D004912:Erythrocytes / Q000000981:diagnostic imaging*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013010:Sonication / Q000295:instrumentation*; D014463:Ultrasonography