[Exogenous nitric oxide alleviates osmotic stress-induced membrane lipid peroxidation in wheat seedling leaves].
When wheat (Triticum aestivum L. Yangmai 158) seedling (with three fully expanded leaves) roots were treated with 15% PEG-6000 in combination with different concentrations (0.1 and 0.5 mmol/L) of exogenous nitric oxide donor sodium nitroprusside (SNP) and NO(-)(3)/NO(-)(2) (control), the enhancement of lipoxygenase (LOX) activity in wheat seedling leaves under osmotic stress was delayed at the lower concentration of SNP treatment (0.1 mmol/L), while the generation rate of O(-.)(2), the enhancement of membrane permeability and the accumulation of MDA and H(2)O(2)were also alleviated. SNP 0.1 mmol/L significantly enhanced the activity of superoxide dismutase (SOD), and accelerated the accumulation of proline. In comparison with these changes, the effects of higher concentration of SNP (0.5 mmol/L) and corresponding control (NO(-)(3)/NO(-)(2)) were not so obvious. The above results indicate that lower concentrations of in vitro nitric oxide (NO) can protect wheat seedling leaves from membrane lipid peroxidation caused by osmotic stress.
Wang
Xian-Ye
XY
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Shen
Wen-Biao
WB
Xu
Lang-Lai
LL
D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008084:Lipoxygenase / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology*; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000378:metabolism*; D011392:Proline / Q000032:analysis; D036226:Seedlings / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014908:Triticum / Q000378:metabolism*
D008563:Membrane Lipids; D013481:Superoxides; D009569:Nitric Oxide; D011392:Proline; D006861:Hydrogen Peroxide; D008084:Lipoxygenase