title: Interactions of soluble guanylate cyclase with diatomics as probed by resonance Raman spectroscopy.
abstract: Soluble guanylate cyclase (sGC, EC 4.6.1.2) acts as a sensor for nitric oxide (NO), but is also activated by carbon monoxide in the presence of an allosteric modulator. Resonance Raman studies on the structure-function relations of sGC are reviewed with a focus on the CO-adduct in the presence and absence of allosteric modulator, YC-1, and substrate analogues. It is demonstrated that the sGC isolated from bovine lung contains one species with a five-coordinate (5c) ferrous high-spin heme with the Fe-His stretching mode at 204 cm(-1), but its CO adduct yields two species with different conformations about the heme pocket with the Fe-CO stretching (nuFe-CO) mode at 473 and 489 cm(-1), both of which are His- and CO-coordinated 6c ferrous adducts. Addition of YC-1 to it changes their population and further addition of GTP yields one kind of 6c (nuFe-CO=489 cm(-1)) in addition to 5c CO-adduct (nuFe-CO=521 cm(-1)). Under this condition the enzymatic activity becomes nearly the same level as that of NO adduct. Addition of gamma-S-GTP yields the same effect as GTP does but cGMP and GDP gives much less effects. Unexpectedly, ATP cancels the effects of GTP. The structural meaning of these spectroscopic observations is discussed in detail.
authors.affiliation: Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Higashiyama 5-1, Okazaki 444-8787, Japan.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Pal
authors.forename: Teizo
authors.initials: T
authors.lastname: Kitagawa
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D002248:Carbon Monoxide / Q000378:metabolism*; D020134:Catalytic Domain; D004789:Enzyme Activation; D006162:Guanylate Cyclase / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013059:Spectrum Analysis, Raman
chemical_list: D009569:Nitric Oxide; D002248:Carbon Monoxide; D006162:Guanylate Cyclase