title: Detection of Chlamydophila pneumoniae in the bone marrow of two patients with unexplained chronic anaemia.
abstract: Anaemia of chronic disease (ACD) is a common finding involving iron deficiency and signs of inflammation. Here, we report on two patients with ACD where a persistent infection with Chlamydophila (Chlamydia) pneumoniae (CP) was detected in bone marrow (BM) biopsies. Infection was suspected by routine cytology and confirmed by immunofluorescence, electron microscopy, polymerase chain reaction (PCR) including different primer sets and laboratories and sequencing of the PCR product. This is a first report of chlamydial presence in the BM of anaemic patients. The cases are presented because persistent chlamydial infection may contribute more frequently to chronic refractory anaemia than previously suspected.
authors.affiliation: Central Laboratory, University Hospital Mannheim, Mannheim, Germany. thomas.nebe@ikc.ma.uni-heidelberg.de
authors.forename: C Thomas
authors.initials: CT
authors.lastname: Nebe
authors.forename: Marion
authors.initials: M
authors.lastname: Rother
authors.forename: Ingrid
authors.initials: I
authors.lastname: Brechtel
authors.forename: Victor
authors.initials: V
authors.lastname: Costina
authors.forename: Michael
authors.initials: M
authors.lastname: Neumaier
authors.forename: Hanswalter
authors.initials: H
authors.lastname: Zentgraf
authors.forename: Ulrich
authors.initials: U
authors.lastname: Böcker
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Meyer
authors.forename: Agnes J
authors.initials: AJ
authors.lastname: Szczepek
mesh_terms: D000328:Adult; D000740:Anemia / Q000209:etiology* / Q000382:microbiology*; D001483:Base Sequence; D001853:Bone Marrow / Q000382:microbiology* / Q000648:ultrastructure; D002690:Chlamydia Infections / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008854:Microscopy, Electron
chemical_list: D004269:DNA, Bacterial