NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly.
The detailed conformational analysis of a single molecule of the tetraacyl biosynthetic precursor-type lipid A and its characteristic supramolecular assembly in aqueous SDS-micelles are described. Regular molecular arrangements were observed by detailed analysis of the NMR spectra of synthetically pure specimens, including regiospecifically 13C-labeled ones. NMR analysis of a biologically inactive precursor-type analogue with four shorter acyl chains demonstrated its conformational flexibility, indicating the importance of hydrophobic interactions for maintaining the conformation of such molecules.
Oikawa
Masato
M
Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.
Shintaku
Tetsuya
T
Fukuda
Naohiro
N
Sekljic
Harald
H
Fukase
Yoshiyuki
Y
Yoshizaki
Hiroaki
H
Fukase
Koichi
K
Kusumoto
Shoichi
S
D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D008050:Lipid A / Q000032:analysis* / Q000096:biosynthesis; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D002241:Carbohydrates; D008050:Lipid A