title: Nonhepatic cell lines HeLa and 293 support efficient replication of the hepatitis C virus genotype 2a subgenomic replicon.
abstract: The hepatitis C virus (HCV) genotype 2a subgenomic replicon can replicate in two human non-hepatocyte-derived cell lines, HeLa and 293, with in vitro-transcribed replicon RNA. Sequencing analysis revealed that mutations in HCV-derived regions were not essential for replication in these cells, as some clones displayed no mutations.
authors.affiliation: Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan.
authors.forename: Takanobu
authors.initials: T
authors.lastname: Kato
authors.forename: Tomoko
authors.initials: T
authors.lastname: Date
authors.forename: Michiko
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Zijiang
authors.initials: Z
authors.lastname: Zhao
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizokami
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
mesh_terms: D002460:Cell Line; D016679:Genome, Viral; D006367:HeLa Cells; D016174:Hepacivirus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D009154:Mutation; D012093:Replicon / Q000235:genetics*; D014779:Virus Replication