MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro.
In this study, the possible effects of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of 1 microM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells.
Kabadere
Selda
S
Department of Physiology, Faculty of Medicine, Osmangazi University, Eskişehir, Turkey.
Oztopçu
Pinar
P
Korkmaz
Seval
S
Erol
Kevser
K
Uyar
Ruhi
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002839:Chromans / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005910:Glioma; D018698:Glutamic Acid / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D008278:Magnesium Sulfate / Q000494:pharmacology*; D051379:Mice; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects*; D010879:Piperazines / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D000975:Antioxidants; D002121:Calcium Channel Blockers; D002839:Chromans; D010879:Piperazines; D013778:Tetrazolium Salts; D013844:Thiazoles; C066382:U 78517F; D018698:Glutamic Acid; D008278:Magnesium Sulfate; C022616:thiazolyl blue