Isolation of Lactobacillus sakei strain KJ-2008 and its removal of characteristic malodorous gases under anaerobic culture conditions.
A number of different sources, such as composts, leachates, and pig feces samples were collected from different pig farms in Korea. Several microorganisms were screened for their ability to deodorize the malodorous gases. As a result, a novel malodorous gas-deodorizing bacterial strain KJ-2008 was isolated due to the most abundant of nitrate-supplemented minimal media under anaerobic conditions. Crimp-sealed serum bottles containing nitrate-supplemented minimal medium (MM-NO(3)(-)) in airtight conditions were inoculated with KJ-2008. Nitrate concentration decreased rapidly after 20 h incubation and nitrite production reached almost zero during the time the experimental was carried out. Taxonomic identification including 16S rDNA base sequencing and phylogenetic analysis indicated that the isolate KJ-2008 had a 99.8% homology in its 16S rDNA base sequence with Lactobacillus sakei. Among the volatile fatty acids, acetic acid contained in large amounts in fresh piggery slurry decreased about 40% after 50 h incubation of the strain KJ-2008. n-Butyric acid, n-valeric acid, and iso-valeric acid gradually decreased, and iso-butyric acid and capronic acid dramatically eliminated at initial time with the treatment. Moreover, NH(3) removal efficiency reached a maximum of 98.5% after 50 h of incubation. The concentration of H(2)S did not change.
Kim
Jeong-Dong
JD
Department of Life Science, Hanyang University, Seoul 133-791, Korea.
Kang
Kook-Hee
KH
D000693:Anaerobiosis; D000818:Animals; D004785:Environmental Pollutants / Q000378:metabolism; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005740:Gases / Q000378:metabolism*; D007723:Korea; D007778:Lactobacillus / Q000302:isolation & purification* / Q000378:metabolism*; D009812:Odorants; D013552:Swine; D018505:Waste Management
D004785:Environmental Pollutants; D005740:Gases