A putative glucan synthase gene dps detected in exopolysaccharide-producing Pediococcus damnosus and Oenococcus oeni strains isolated from wine and cider.
Some lactic acid bacteria can induce viscosity in wine, beer and cider by production of exopolysaccharides (EPS). A polymerase chain reaction (PCR) assay was previously described for the detection of ropy Pediococcus damnosus strains in wine [J. Appl. Microbiol. 90 (2001) 535]. The primers used in that study, PF5 and PF6, are investigated in addition to new primers which broaden the range of spoiling agents detectable by PCR. Primers PF1 and PF8 allow the amplification of DNA from ropy P. damnosus strains isolated from wine, as was observed with PF5 and PF6. In addition, PF1 and PF8, unlike PF5 and PF6, are able to generate an amplicon using template DNA from a ropy P. damnosus strain isolated from cider and a ropy Oenococcus oeni strain isolated from champagne. Two different ropy Lactobacillus species were also isolated, but their DNA was not amplified using primers PF1 and PF8. The new primers PF1 and PF8 were chosen from the sequence of gene dps, a putative glucan synthase gene, found across all the ropy P. damnosus strains isolated, from both wine or cider, and also in a ropy O. oeni strain. To our knowledge, this is the first time that an EPS-producing O. oeni strain is described. Glucan biosynthesis was assessed by agglutination tests done with Streptococcus pneumoniae type 37-specific antibodies, which specifically detect glucan-producing cells. The results show that there is a direct correlation between glucan production and detection of gene dps. Therefore, Dps is considered a candidate for the glucan synthase enzyme responsible for EPS production by ropy strains of P. damnosus and O. oeni.
Walling
Emilie
E
Laboratoire de Biotechnologie et Microbiologie Appliquée, Faculté d'Oenologie, Unité Associée INRA-Université Victor Segalen Bordeaux 2, 33405 Talence, France. emilie.walling@etud.u-bordeaux2.fr
Gindreau
Emmanuel
E
Lonvaud-Funel
Aline
A
D000372:Agglutination Tests; D001515:Beer / Q000382:microbiology; D001628:Beverages / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D005516:Food Microbiology; D005964:Glucosyltransferases / Q000235:genetics*; D007218:Industrial Microbiology; D010376:Pediococcus / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014920:Wine / Q000382:microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D005964:Glucosyltransferases; C040232:glucan synthase