[Determination of epimedin C and icariin in Herba Epimedii by HPLC].
OBJECTIVE
To establish a RP-HPLC method for the determination of epimedin C and icariin in Herba Epimedii.


METHODS
Chromatographic conditions: Hypersil BDS-C18 column, acetonitrice and water containing 0.05% phosphoric acid as gradient eluents, G1315A photodiode array detector at 272 nm.


RESULTS
The average recovery rate of epimedin C was 99.7%, RSD 1.5% (n = 9), icariin 102.5%, RSD 1.1% (n=9).


CONCLUSIONS
The method is reliable, stable and well repeatable. It provides the useful information for quality evaluation.
Wang
Ming-quan
MQ
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, Jiansu, China.
Bi
Zhi-ming
ZM
Li
Ping
P
Ji
Hui
H
Cheng
Fei-long
FL
D002681:China; D004365:Drugs, Chinese Herbal / Q000032:analysis; D017753:Ecosystem; D034181:Epimedium / Q000737:chemistry* / Q000145:classification; D005419:Flavonoids / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013045:Species Specificity
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C106054:epimedin C; C056599:icariin