Combined haploid and insertional mutation screen in the zebrafish.
To identify genes required for development of the brain and somites, we performed a pilot screen of gynogenetic haploid zebrafish embryos produced from mothers mutagenized by viral insertion. We describe an efficient method to identify new mutations and the affected gene. In addition, we report the results of a small-scale screen that identified five genes required for brain development, including novel alleles of nagie oko, pou5f1, ribosomal protein L36, and n-cadherin, as well as a novel allele of the laminin g1 gene that is required for normal skeletal muscle fiber organization and somite patterning.
Wiellette
Elizabeth
E
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Grinblat
Yevgenya
Y
Austen
Matthias
M
Hirsinger
Estelle
E
Amsterdam
Adam
A
Walker
Charline
C
Westerfield
Monte
M
Sive
Hazel
H
D000483:Alleles; D000818:Animals; D019521:Body Patterning; D001921:Brain / Q000196:embryology; D003001:Cloning, Molecular; D005260:Female; D006162:Guanylate Cyclase / Q000235:genetics; D006238:Haploidy; D008297:Male; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000379:methods*; D009154:Mutation; D010641:Phenotype; D019170:Somites; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
D029961:Zebrafish Proteins; C501511:mpp5a protein, zebrafish; D006162:Guanylate Cyclase