[Immunoscreening of newborn larvae cDNA library of Trichinella spiralis].
OBJECTIVE
To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis.


METHODS
Newborn larvae (NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed.


RESULTS
158 positive clones were obtained from 4.5 x 10(5) recombinant clones by immunoscreening. The sequence analysis of positive clones showed that there are 36 novel cDNAs, 5 reported cDNAs and 12 cDNAs without the ORF showing high similarity to the mitochondrial DNA of Trichinella spiralis.


CONCLUSIONS
Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.
Wu
Xiu-ping
XP
Faculty of Military Veterinary, Quartermaster University of PLA, Changchun 130062, China.
Fu
Bao-quan
BQ
Liu
Ming-yuan
MY
Zhang
Ya-lan
YL
Yuan
Li-hong
LH
Li
Lian-rui
LR
Lu
Qiang
Q
Boireau
Pascal
P
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D006801:Humans; D007814:Larva / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013552:Swine; D017160:Trichinella spiralis / Q000235:genetics* / Q000276:immunology*
D000947:Antigens, Helminth; D018076:DNA, Complementary