title: Monitoring the formation of biosilica catalysed by histidine-tagged silicatein.
abstract: Surface bound silicatein retains its biocatalytic activity, which was demonstrated by monitoring the immobilisation of silicatein using a histidine-tag chelating anchor and the subsequent biosilicification of SiO(2) on surfaces by surface plasmon resonance spectroscopy, atomic force microscopy and scanning electron microscopy.
authors.affiliation: Institut für Anorganische Chemie, Universität Mainz, Duesbergweg 10-14, D-55128 Mainz, Germany.
authors.forename: Muhammad Nawaz
authors.initials: MN
authors.lastname: Tahir
authors.forename: Patrick
authors.initials: P
authors.lastname: Théato
authors.forename: Werner E G
authors.initials: WE
authors.lastname: Müller
authors.forename: Heinz C
authors.initials: HC
authors.lastname: Schröder
authors.forename: Andreas
authors.initials: A
authors.lastname: Janshoff
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Joachim
authors.initials: J
authors.lastname: Huth
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Tremel
mesh_terms: D002384:Catalysis; D002403:Cathepsins / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D006639:Histidine; D012822:Silicon Dioxide; D002403:Cathepsins