[A two-dimensional reference map of mouse ovary proteins].
OBJECTIVE
To perform a preliminary proteomic analysis of mouse ovaries and to study the protein's function in mouse ovary.


METHODS
The two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) were used to analyze mouse ovarian proteome. A 12.5% sodium dodecyl sulfate (SDS) reference gel was generated by immobilized pH gradient isoelectric focusing of mouse ovary proteins in a non-linear gradient (pH 3-10). And GRP78 was selected to perform with immunohistochemistry within mouse ovaries.


RESULTS
Based on peptide mass fingerprinting, 52 proteins were identified and classified into seven functional groups: Cell/organism defense and antioxidant, cell signaling/communications proteins, cell structure/motility proteins, metabolism proteins, RNA synthesis processing, protein synthesis and processing, and unclassified proteins. The immunoreactivity of GRP78 was detected in GCs in the follicular, and with during GCs Luteinizing in the menstrual cycle, the protein expression (brown) increased continually and came to a head when ovulation happened.


CONCLUSIONS
This work provides a first step toward the establishment of a systematic ovary protein database and stands as a valuable resource for molecular analyses of normal and pathologic conditions affecting mouse ovaries.
Ma
Xiang
X
Center of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Qian
Yun
Y
Xie
Man-xin
MX
Hou
Zhen
Z
Meng
Yan
Y
Liu
Jin-yong
JY
Zhai
Yong-ning
YN
Zhu
Ye-fei
YF
Zhou
Zuo-min
ZM
Sha
Jia-hao
JH
Liu
Jia-yin
JY
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D006360:Heat-Shock Proteins / Q000032:analysis; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D018832:Molecular Chaperones / Q000032:analysis; D010053:Ovary / Q000737:chemistry* / Q000166:cytology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D018832:Molecular Chaperones; D020543:Proteome