Formation of the aureothin tetrahydrofuran ring by a bifunctional cytochrome p450 monooxygenase.
The polyketide antibiotic aureothin, produced by Streptomyces thioluteus, is equipped with a rare exomethylene-tetrahydrofuran moiety. Cloning, heterologous expression, and inactivation experiments reveal that AurH, a bifunctional cytochrome P450 monooxygenase, is required and sufficient for the stereoselective synthesis of the furan ring, involving the subsequent formation of two new C-O bonds.
He
Jing
J
Hans-Knöll-Institute for Natural Products Research, Beutenbergstrasse 11a, 07745 Jena, Germany.
Müller
Markus
M
Hertweck
Christian
C
D002867:Chromones / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D005663:Furans / Q000378:metabolism*; D005810:Multigene Family; D048170:Streptomyces lividans / Q000235:genetics / Q000378:metabolism
D002867:Chromones; D005663:Furans; D003577:Cytochrome P-450 Enzyme System; C012198:aureothin