title: Antiproliferative effect of mineral trioxide aggregate, zinc oxide-eugenol cement, and glass-ionomer cement against three fibroblastic cell lines.
abstract: An important requirement for dental materials placed in direct contact with living tissues is biocompatibility. The purpose of this study was to evaluate the antiproliferative activity of three dental materials (mineral trioxide aggregate, zinc oxide-eugenol cement, and glass-ionomer cement) against a panel of established fibroblastic cell lines (L929, BHK21/C13, and RPC-C2A). The materials were prepared according to the manufacturer's instructions and were tested in insert wells for 12, 24, and 48 h. Cell number fraction was estimated by the sulforhodamine-B assay, in reference to controls. The degree of antiproliferative effect in ascending order was mineral trioxide aggregate, glass-ionomer cement, and zinc oxide-eugenol cement in all cell lines tested.
authors.affiliation: Department of Operative Dentistry, Dental School, Aristotle University Thessaloniki, Greece. koulaouz@dent.auth.gr
authors.forename: Elisabeth A
authors.initials: EA
authors.lastname: Koulaouzidou
authors.forename: Konstantinos T
authors.initials: KT
authors.lastname: Papazisis
authors.forename: Nikolaos A
authors.initials: NA
authors.lastname: Economides
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Beltes
authors.forename: Alexander H
authors.initials: AH
authors.lastname: Kortsaris
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D006224:Cricetinae; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000633:toxicity; D008422:Materials Testing; D008768:Methylmethacrylates / Q000633:toxicity; D051379:Mice; D010087:Oxides / Q000633:toxicity; D051381:Rats; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D005899:Glass Ionomer Cements; D008768:Methylmethacrylates; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C084541:Fuji glass-ionomer lining cement; C031667:IRM cement