title: Intravenous catecholamine administration affects mouse intestinal lymphocyte number and apoptosis.
abstract: The purposes of this study were to determine plasma and intestinal epinephrine (E) and norepinephrine (NE) concentrations in mice after exercise stress and, the effect of intravenous injection of E and NE (at concentrations during exercise) on viability of intestinal lymphocytes (IL). Exhaustive exercise significantly elevated plasma E and NE, and intestinal E, compared with sedentary animals. Twenty-four hours after intravenous NE administration, IL counts were higher (p<0.001) and % apoptotic IL were lower (p<0.001) than saline conditions. E resulted in fewer apoptotic IL at 24 h compared to saline controls. E and NE differentially influence IL numbers at 24 h after injection although both result in fewer % apoptotic IL relative to mice given saline only.
authors.affiliation: Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: S
authors.initials: S
authors.lastname: Marra
authors.forename: M
authors.initials: M
authors.lastname: Burnett
authors.forename: L
authors.initials: L
authors.lastname: Hoffman-Goetz
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D004837:Epinephrine / Q000097:blood / Q000633:toxicity*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D007275:Injections, Intravenous / Q000379:methods; D007422:Intestines / Q000166:cytology*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000097:blood / Q000633:toxicity*; D010641:Phenotype; D010805:Physical Conditioning, Animal / Q000379:methods; D011419:Propidium; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011897:Random Allocation; D014018:Tissue Distribution
chemical_list: D017304:Annexin A5; D019253:Proto-Oncogene Proteins c-bcl-2; D011419:Propidium; D017493:Leukocyte Common Antigens; D009638:Norepinephrine; D004837:Epinephrine