title: Systematic identification and molecular characterization of genes differentially expressed in breast and ovarian cancer.
abstract: The identification of novel disease-associated genes in gynaecological tumours has important implications for understanding the process of tumourigenesis and the development of novel treatment regimens. cDNA libraries from disease tissues may represent a valuable source to identify such genes. Recently, a bio-informatic procedure based on an 'electronic Northern' approach was established to screen expressed sequence tag (EST) libraries for genes differentially expressed in tumour and normal tissues, and identified 450 candidate genes differentially expressed in breast and ovarian cancer. In this report, the validation of an initial set of 40 candidate genes, which were selected due to their localization in chromosomal regions frequently altered in gynaecological tumours, is described. Differential expression of 29 of these genes, including three uncharacterized novel genes, was confirmed by applying cancer profiling arrays with 106 matched pairs of tumour/normal cDNAs and quantitative reverse transcription-polymerase chain reaction (RT-PCR) on 60 clinical specimens. The majority of these differentially expressed genes have not been described previously in the context of breast and ovarian cancer, and may constitute novel diagnostic markers for these tumour entities.
authors.affiliation: Institute of Pathology, RWTH Aachen, Germany. edahl@ukaachen.de
authors.forename: Edgar
authors.initials: E
authors.lastname: Dahl
authors.forename: Ariane
authors.initials: A
authors.lastname: Sadr-Nabavi
authors.forename: Eva
authors.initials: E
authors.lastname: Klopocki
authors.forename: Beate
authors.initials: B
authors.lastname: Betz
authors.forename: Susanne
authors.initials: S
authors.lastname: Grube
authors.forename: Rene
authors.initials: R
authors.lastname: Kreutzfeld
authors.forename: Marina
authors.initials: M
authors.lastname: Himmelfarb
authors.forename: Han-Xiang
authors.initials: HX
authors.lastname: An
authors.forename: Stephen
authors.initials: S
authors.lastname: Gelling
authors.forename: Irina
authors.initials: I
authors.lastname: Klaman
authors.forename: Bernd
authors.initials: B
authors.lastname: Hinzmann
authors.forename: Glen
authors.initials: G
authors.lastname: Kristiansen
authors.forename: Robert
authors.initials: R
authors.lastname: Grützmann
authors.forename: Ruprecht
authors.initials: R
authors.lastname: Kuner
authors.forename: Beate
authors.initials: B
authors.lastname: Petschke
authors.forename: Kerstin
authors.initials: K
authors.lastname: Rhiem
authors.forename: Kai
authors.initials: K
authors.lastname: Wiechen
authors.forename: Christine
authors.initials: C
authors.lastname: Sers
authors.forename: Otmar
authors.initials: O
authors.lastname: Wiestler
authors.forename: Achim
authors.initials: A
authors.lastname: Schneider
authors.forename: Heinz
authors.initials: H
authors.lastname: Höfler
authors.forename: Jörg
authors.initials: J
authors.lastname: Nährig
authors.forename: Manfred
authors.initials: M
authors.lastname: Dietel
authors.forename: Reinhold
authors.initials: R
authors.lastname: Schäfer
authors.forename: André
authors.initials: A
authors.lastname: Rosenthal
authors.forename: Rita
authors.initials: R
authors.lastname: Schmutzler
authors.forename: Matthias
authors.initials: M
authors.lastname: Dürst
authors.forename: Alfons
authors.initials: A
authors.lastname: Meindl
authors.forename: Dieter
authors.initials: D
authors.lastname: Niederacher
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005819:Genetic Markers; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D018076:DNA, Complementary; D004273:DNA, Neoplasm; D005819:Genetic Markers; D009363:Neoplasm Proteins