title: Side-chain conformational restriction in template-competitive inhibitors of E. coli DNA polymerase I Klenow fragment: synthesis, structural characterization and inhibition activity.
abstract: Nucleotide triphosphate alpha-(4-azidophenyl)-1,N6-etheno-dATP 3 and its monophosphate 3m were synthesized by condensation of 2-halo-2-(4-azidophenyl)acetaldehyes with dATP and dAMP, respectively. Structure analysis shows that the azidophenyl side chain is attached to the alpha-position of the etheno ring (i.e., the carbon attached to N1 of the purine), and conformation calculations show minima in the etheno-phenyl bond rotation at 50 and 130 degrees where the bulk of the phenyl ring projects out from the plane of the etheno group. Like DNA Pol inhibitor 2-(4-azidophenacyl)thio-2'-deoxyadenosine 5'-triphosphate 1, nucleotide 3 is a template-competitive DNA polymerase inhibitor (TCPI), with a competitive Ki for Pol I KF of 3.41 microM, but has only weak activity as an HIV RT inhibitor relative to the template-competitive reverse transcriptase inhibitor 2-(4-azidophenacyl)thio-1,N6-etheno-2'-deoxyadenosine 5'-triphosphate 2. Additionally, 3 photoinactivates KF in a time-dependent manner, confirming the kinetic data that 3 binds to the free form of KF. The TCPI activity of 3 provides evidence for an extended side chain conformational preference in the combined substrate polymerase inhibitors.
authors.affiliation: Department of Chemistry and Physics, Southeastern Louisiana University, Hammond, Louisiana 70402, USA. mdoughty@selu.edu
authors.forename: Michael B
authors.initials: MB
authors.lastname: Doughty
authors.forename: Karam
authors.initials: K
authors.lastname: Aboudehen
authors.forename: Garland
authors.initials: G
authors.lastname: Anderson
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Bob
authors.initials: B
authors.lastname: Moore
authors.forename: Tina
authors.initials: T
authors.lastname: Poolson
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives; D001386:Azides / Q000737:chemistry / Q000378:metabolism; D001667:Binding, Competitive; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors* / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006801:Humans; D019800:Phenol / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D018894:Reverse Transcriptase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001386:Azides; D004791:Enzyme Inhibitors; D018894:Reverse Transcriptase Inhibitors; D019800:Phenol; D054303:HIV Reverse Transcriptase; D004256:DNA Polymerase I; D000241:Adenosine