Proteomic analysis of Deinococcus radiodurans recovering from gamma-irradiation.
In order to reveal the mechanisms of the extreme radioresistance and DNA repair in Deinococcus radiodurans, we examined proteome changes in a wild-type strain following gamma-irradiation using two-dimensional polyacrylamide gel electrophoresis and Silver-staining. The expression levels of 26 protein spots showed significant changes under radiation stress. Of these spots, 21 were identified with peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight mass spectrometry after tryptic in-gel digestion. These proteins exhibited various cellular functions, including (i) translation; (ii) transcription; (iii) signal transduction; (iv) post-translational modification, protein turnover, chaperones; (v) carbohydrate transport and metabolism; (vi) energy production and conversion; (vii) nucleotide transport and metabolism; (viii) inorganic ion transport and metabolism; (ix) DNA replication, recombination and repair; and (x) yet unknown. Most of the proteins have not previously been reported to be relevant to radioresistance.
Zhang
Chunchao
C
Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, China.
Wei
Jianfeng
J
Zheng
Zhiguo
Z
Ying
Nanjiao
N
Sheng
Duohong
D
Hua
Yuejin
Y
D001426:Bacterial Proteins / Q000737:chemistry*; D034301:Deinococcus / Q000737:chemistry* / Q000528:radiation effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005720:Gamma Rays; D010449:Peptide Mapping; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins