Effects induced by Tityus serrulatus scorpion venom and its toxins TsTX-I and TsTX-V on the rat isolated retractor penis muscle.
The aims of the present study were to investigate the pharmacological effects induced by Tityus serrulatus venom (TsV) and its fractions and to compare with the effects induced by pure alpha (TsTX-V) and beta (TsTX-I) toxins isolated from TsV on rat retractor penis muscle (RPM). TsV, fractions X, XI, XIIa, XIIb (0.01-100 microg/ml) and TsTX-V (1 nmol/l-10 micromol/l) induced concentration-dependent contractions. Prazosin and guanethidine or tetrodotoxin (TTX, 5 micromol/l, 30 min) completely abolished these contractions, suggesting complete dependence on sympathetic nerves. TsV or fractions X, XI, XIIa, XIIb (0.01- 100 microg/ml), TsTX-I and TsTX-V (1 nmol/l-10 micromol/l) induced concentration-dependent relaxations in the precontracted RPM. TTX or N(omega)-nitro-L-arginine methyl ester (L-NAME, 100 micromol/l, 30 min) completely abolished the relaxations. Our results suggest that most of TsV-derivated toxins induce contraction and relaxation on RPM by sympathetic and NANC nitrergic nerve stimulation. Noteworthy, TsTX-I only induces relaxation on RPM suggesting that this protein selectively acts on inhibitory nerves.
Bomfim
José Henrique G G
JH
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Brasil.
de Godoy
Márcio A F
MA
Giglio
José R
JR
de Oliveira
Ana Maria
AM
Arantes
Eliane C
EC
D000818:Animals; D018723:Bethanechol / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D006145:Guanethidine / Q000494:pharmacology; D019476:Insect Proteins; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000473:pathology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D010108:Oxyhemoglobins / Q000494:pharmacology; D010413:Penis / Q000033:anatomy & histology / Q000187:drug effects / Q000473:pathology*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D012604:Scorpion Venoms / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D012605:Scorpions; D020539:Sequence Analysis, Protein; D013779:Tetrodotoxin / Q000494:pharmacology
D019476:Insect Proteins; D009498:Neurotoxins; D010108:Oxyhemoglobins; D012604:Scorpion Venoms; C093405:scorpion toxin CsE-V; C120263:scorpion toxin Ts1, Tityus; C033843:toxin V; D018723:Bethanechol; D013779:Tetrodotoxin; D019331:NG-Nitroarginine Methyl Ester; D011224:Prazosin; D006145:Guanethidine