A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides.
The application of recombinant antibodies in plant biology research is limited because plant researchers have minimal access to high-quality phage display libraries. Therefore, we constructed a library of 1.3 x 10(10) clones displaying human single-chain variable fragments (scFvs) that is available to the academic community. The scFvs selected from the library against a diverse set of plant proteins showed moderate to high antigen-binding affinity together with high specificity. Moreover, to optimize an scFv as immunodetection agent, two expression systems that allow efficient production and purification of bivalent scFv-Fc and scFv-CkappaZIP fusion proteins were integrated. We are convinced that this antibody platform will further stimulate applications of recombinant antibodies such as the diagnostic detection or immunomodulation of specific antigens in plants.
Eeckhout
Dominique
D
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Technologiepark 927, B-9052 Gent, Belgium.
De Clercq
Annelies
A
Van De Slijke
Eveline
E
Van Leene
Jelle
J
Stals
Hilde
H
Casteels
Peter
P
Persiau
Geert
G
Vercammen
Dominique
D
Van Breusegem
Frank
F
Zabeau
Marc
M
Inzé
Dirk
D
Jespers
Laurent
L
Depicker
Ann
A
De Jaeger
Geert
G
D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D000915:Antibody Affinity / Q000235:genetics* / Q000276:immunology; D002352:Carrier Proteins / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D010455:Peptides / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013379:Substrate Specificity / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D010455:Peptides; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins