title: Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number.
abstract: We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGE-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGE-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGE-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGE-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >10(5) copies of LAGE-1a mRNA.
authors.affiliation: Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Sato
authors.forename: Yuji
authors.initials: Y
authors.lastname: Noguchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Wada
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Fujita
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Ryo
authors.initials: R
authors.lastname: Tanaka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakada
authors.forename: Kosei
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Fumihito
authors.initials: F
authors.lastname: Koizumi
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ono
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nouso
authors.forename: Achim
authors.initials: A
authors.lastname: Jungbluth
authors.forename: Yao-Tseng
authors.initials: YT
authors.lastname: Chen
authors.forename: Lloyd J
authors.initials: LJ
authors.lastname: Old
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shiratori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Nakayama
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger