title: Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits.
abstract: We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P<0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.
authors.affiliation: College of Veterinary Medicine and Bio-Safety Research Center, Chonbuk National University, Jeonju 561-756, Korea. tick@chonbuk.ac.kr
authors.forename: Myung-Jo
authors.initials: MJ
authors.lastname: You
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D019476:Insect Proteins / Q000276:immunology; D026863:Ixodidae / Q000276:immunology*; D008840:Microfilament Proteins / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology*; D013984:Tick Infestations / Q000276:immunology* / Q000517:prevention & control
chemical_list: D000906:Antibodies; D019476:Insect Proteins; D008840:Microfilament Proteins; C444437:P27-30 protein, Haemaphysalis longicornis; D011994:Recombinant Proteins