Equine herpes virus 2 infection in horse populations in Poland.
The prevalence of Equine herpesvirus 2 (EHV-2) infections in the horse populations in Poland was investigated. Peripheral blood leukocytes (PBLs) of 139 horses were tested. The animals were divided into four groups: clinically healthy horses, horses suffering from respiratory disorders, mares with a recent abortion and horses with diagnosed ataxia. Thirty-four virus isolates were obtained from leukocytes of the tested animals by cocultivation with equine dermal cells and were identified as EHV-2 by PCR using primers for the gB gene of EHV-2 and/or primers for the sequence located upstream of the gene homologous to the equine interleukin 10 (IL-10) gene. These results indicate that EHV-2 is prevalent in horse populations in Poland. As the virus was most frequently isolated from horses with respiratory disorders its etiological importance may be considered.
Ruszczyk
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Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland. ruszczyk@alpha.sggw.waw.pl
Cywinska
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Banbura
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D000818:Animals; D018920:Coculture Techniques; D017931:DNA Primers; D005260:Female; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D006736:Horses; D016753:Interleukin-10 / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000821:virology; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012140:Respiratory Tract Diseases / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D018144:Rhadinovirus / Q000235:genetics / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics
D017931:DNA Primers; D014759:Viral Envelope Proteins; D016753:Interleukin-10