title: E1-Ngn2/Cre is a new line for regional activation of Cre recombinase in the developing CNS.
abstract: We generated a transgenic mouse line named E1-Ngn2/Cre that expresses Cre recombinase and GFP under the control of the E1 enhancer element of the gene Ngn2 (Scardigli et al.: Neuron 31:203-217, 2001). Cre-recombinase activity and GFP fluorescence are consistent with the reported expression pattern controlled by the E1-Ngn2 enhancer. Recombination was detected in the progenitor domains p1 and p2 in the ventricular zone of the neural tube and in distinct domains of the pretectum, the dorsal and ventral thalamus, the tegmentum of the mesencephalon, and the hindbrain. In the developing cortex, Cre-recombinase activity is confined to a subpopulation of progenitors predominantly in the region of the ventral and lateral pallium. The E1-Ngn2/Cre mouse line thus provides an excellent novel tool for a region-specific conditional mutagenesis in the developing CNS.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Berger
authors.forename: Silke
authors.initials: S
authors.lastname: Eckert
authors.forename: Raffaella
authors.initials: R
authors.lastname: Scardigli
authors.forename: François
authors.initials: F
authors.lastname: Guillemot
authors.forename: Peter
authors.initials: P
authors.lastname: Gruss
authors.forename: Anastassia
authors.initials: A
authors.lastname: Stoykova
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology* / Q000201:enzymology; D004742:Enhancer Elements, Genetic; D004970:Estrone / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D011995:Recombination, Genetic
chemical_list: C092751:estra-1,3,5(10)-trien-17-one-3-amine; D049452:Green Fluorescent Proteins; D004970:Estrone; C045073:Cre recombinase; D019426:Integrases