[Effect of autologous dendritic cells on chronic myeloid leukemia cells in vitro].
OBJECTIVE
To observe the effect of autologous dendritic cells (DC) on positive Philadelphia (Ph+) chromosome cells of Chronic Myeloid Leukemia (CML) in vitro.


METHODS
DCs and T cells extracted from the peripheral blood mononuclear cell (PBMNC) of 4 CML patients with Ph+ chromosome in chronic phases were amplified in a special liquid culture system. The survival rates of the Ph+ chromosome cells in the bone marrow mononuclear cells (BMMNC) and the K562 cells were determined by the conventional chromosome assays, after About 3 x 10(5)/ml DC incubating with autologous T cells activated by IL-2 or DCs plus IL-2 for 6 hours.


RESULTS
About 3 x 10(5)/ml DC per 2 x 10(6)/ml PBMNC were produced. The cytotoxic effect of the T cells activated by DCs plus IL-2 on the autologous Ph+ chromosome cells was significantly greater than those activated by IL-2 (P < 0.001). The clearance activity of the DC activated T cells on the autologous CML Ph+ chromosome cells was stronger than on the K562 cells (P < 0.001).


CONCLUSIONS
The DCs-activated autologous T cells can effectively inhibit the Ph+ chromosome cells. The cytotoxic effect of the autologous T cells activated by the DCs on the Ph+ chromosome cells could be MHC-restricted.
Li
Yun
Y
Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang
Yiming
Y
Li
Shengfu
S
Meng
Wentong
W
Ding
Xinmin
X
Wu
Yu
Y
D000328:Adult; D002450:Cell Communication; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D015466:Leukemia, Myeloid, Chronic-Phase / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology*; D014407:Tumor Cells, Cultured