Effects of TCDD in modulating the expression of Sertoli cell secretory products and markers for cell-cell interaction.
Among different mammalian tissues, testis is found to be one of the most sensitive organs to TCDD exposure. In this study, primary Sertoli cell culture was established. The purity of the cultured cells was verified using 3beta-hydroxysteroid dehydrogenase, alkaline phosphatase as well as testosterone induction assays. Effects of TCDD in modulating the expression of CYP1A1, aromatase, secretory products (i.e. Mullerian inhibiting substance (MIS), 17beta-estradiol (E(2)) and lactate) and markers for cell-cell interaction (i.e. sertolin and testin) were then examined. Our data demonstrated that Sertoli cells exposed to 0.2-2000 pg/ml of TCDD showed a dose dependent induction of CYP1A1 mRNA. The minimal dose of activation was 2 pg/ml, which indicated that the cell was very sensitive to TCDD exposure. However, there was little or no detectable level CYP1A1 protein and EROD activities found. Dose-dependent inductions of aromatase transcript (200%) and E(2) (20%) secretion were measured. In addition there was a significant reduction (40%) of MIS mRNA. No detectable change in the level of secreted lactate was observed. Sertolin and testin, the gene makers for cell-cell interactions were differentially modulated upon TCDD treatment. Taken together, the results implicated that TCDD exposure might interfere with the normal Sertoli cell functions.
Lai
K P
KP
Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.
Wong
M H
MH
Wong
Chris K C
CK
D000818:Animals; D054304:Anti-Mullerian Hormone; D001141:Aromatase / Q000096:biosynthesis; D015153:Blotting, Western; D002450:Cell Communication / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D004958:Estradiol / Q000096:biosynthesis; D006023:Glycoproteins / Q000096:biosynthesis; D019344:Lactic Acid / Q000096:biosynthesis; D008297:Male; D010455:Peptides / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism*; D013735:Testicular Hormones / Q000096:biosynthesis
D004785:Environmental Pollutants; D006023:Glycoproteins; D010455:Peptides; D000072317:Polychlorinated Dibenzodioxins; D011506:Proteins; D013735:Testicular Hormones; C121678:sertolin; C489180:testin protein, rat; D019344:Lactic Acid; D004958:Estradiol; D054304:Anti-Mullerian Hormone; D001141:Aromatase; D019363:Cytochrome P-450 CYP1A1