title: Proteomic analysis of human natural killer cells: insights on new potential NK immune functions.
abstract: Applying high-throughput proteomic analysis of mammalian cells can facilitate the identification of a large number of proteins expressed in the examined samples. Moreover, extensive research efforts are being made to perform large-scale characterization of membrane proteins. Here we use mass spectrometry-based proteomic strategy to characterize protein expression in membrane-enriched fractions derived from human NK lymphoma cell line YTS. This query yielded a list of over 1000 identified proteins, and provided us with new insights on NK cell biology. We highlight the expression of CD86 on YTS and its ability to co-stimulate TCR responses of human CD4+ T-cells, providing an unexpected link between innate and adaptive immune systems.
authors.affiliation: The Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
authors.forename: Jacob
authors.initials: J
authors.lastname: Hanna
authors.forename: Jonathan
authors.initials: J
authors.lastname: Fitchett
authors.forename: Tony
authors.initials: T
authors.lastname: Rowe
authors.forename: Mark
authors.initials: M
authors.lastname: Daniels
authors.forename: Melissa
authors.initials: M
authors.lastname: Heller
authors.forename: Tsufit
authors.initials: T
authors.lastname: Gonen-Gross
authors.forename: Efrat
authors.initials: E
authors.lastname: Manaster
authors.forename: Samuel Y
authors.initials: SY
authors.lastname: Cho
authors.forename: Michael J
authors.initials: MJ
authors.lastname: LaBarre
authors.forename: Ofer
authors.initials: O
authors.lastname: Mandelboim
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D051940:B7-2 Antigen; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D011948:Receptors, Antigen, T-Cell / Q000276:immunology
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell