Loss of subcutaneous adipose tissue in HIV-associated lipodystrophy is not due to accelerated apoptosis.
HIV-associated lipodystrophy is characterized by a loss of adipose tissue from the subcutaneous compartment. Previously reported data suggested that this loss of adipose tissue was the result of an increased rate of apoptosis in subcutaneous adipose tissue. The present study examined the rate of apoptosis in subcutaneous adipose tissue with a sensitive ligase-mediated polymerase chain reaction technique to amplify DNA ladders. Individuals with HIV lipodystrophy were compared with HIV-infected subjects without lipodystrophy and subjects without HIV disease. Although apoptosis was observed in subjects with HIV lipodystrophy, there was no difference in the frequency of individuals with apoptosis among those with HIV lipodystrophy (10/22), those with HIV but no lipodystrophy (13/25), and subjects without HIV disease (13/27).
Mynarcik
Dennis
D
Department of Medicine, Division of Endocrinology, State University of New York at Stony Brook, Stony Brook, NY, USA.
Wei
Lin-Xiang
LX
Komaroff
Eugene
E
Ferris
Robert
R
McNurlan
Margaret
M
Gelato
Marie
M
D017667:Adipocytes / Q000473:pathology; D000273:Adipose Tissue / Q000473:pathology*; D000328:Adult; D017209:Apoptosis; D016022:Case-Control Studies; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation; D005260:Female; D015658:HIV Infections / Q000473:pathology; D039682:HIV-Associated Lipodystrophy Syndrome / Q000473:pathology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D040521:Subcutaneous Tissue / Q000473:pathology*
D004247:DNA