title: Analysis of the major patterns of B cell gene expression changes in response to short-term stimulation with 33 single ligands.
abstract: We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca(2+) and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L.
authors.affiliation: Molecular Biology Laboratory, Alliance for Cellular Signaling, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Zhu
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hart
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Chang
authors.forename: Jong-Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Yun Anna
authors.initials: YA
authors.lastname: Cao
authors.forename: Dennis
authors.initials: D
authors.lastname: Mock
authors.forename: Eugene
authors.initials: E
authors.lastname: Ke
authors.forename: Brian
authors.initials: B
authors.lastname: Saunders
authors.forename: Angela
authors.initials: A
authors.lastname: Alexander
authors.forename: Joella
authors.initials: J
authors.lastname: Grossoehme
authors.forename: Keng-Mean
authors.initials: KM
authors.lastname: Lin
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.forename: Robert
authors.initials: R
authors.lastname: Hsueh
authors.forename: Jamie
authors.initials: J
authors.lastname: Lee
authors.forename: Richard H
authors.initials: RH
authors.lastname: Scheuermann
authors.forename: David A
authors.initials: DA
authors.lastname: Fruman
authors.forename: William
authors.initials: W
authors.lastname: Seaman
authors.forename: Shankar
authors.initials: S
authors.lastname: Subramaniam
authors.forename: Paul
authors.initials: P
authors.lastname: Sternweis
authors.forename: Melvin I
authors.initials: MI
authors.lastname: Simon
authors.forename: Sangdun
authors.initials: S
authors.lastname: Choi
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism / Q000494:pharmacology; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D023201:CD40 Ligand / Q000378:metabolism / Q000494:pharmacology; D049109:Cell Proliferation; D002478:Cells, Cultured; D018899:CpG Islands / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000276:immunology*; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology; D008024:Ligands; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000235:genetics* / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D008024:Ligands; D008070:Lipopolysaccharides; D023201:CD40 Ligand; D015847:Interleukin-4