title: Estrogen promotes differentiation and survival of dopaminergic neurons derived from human neural stem cells.
abstract: To investigate the effect of estrogen on neuronal differentiation, especially on dopaminergic (DA) neurons, human neural stem cells (NSCs) were differentiated in the presence of 17beta-estradiol. NSCs gave rise to tyrosine hydroxylase (TH)-positive neurons in vitro, the proportion of which was increased by 17beta-estradiol. Increase in TH-positive neurons was abrogated by an estrogen receptor (ER) antagonist, ICI182780, suggesting ERs play a role in differentiation of DA neurons. The observation that ERs were expressed in both proliferating NSCs and postmitotic DA neurons suggested that increase in TH-positive neurons was due to induction and support of DA neurons. 17beta-Estradiol also increased the number of DA neurons derived from human NSCs in vivo when the cells were grafted into mouse brains. These results support a possible role for estrogen in the transplantation of NSCs for Parkinson's disease.
authors.affiliation: Department of Neurosurgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Yo
authors.initials: Y
authors.lastname: Kishi
authors.forename: Jun
authors.initials: J
authors.lastname: Takahashi
authors.forename: Masaomi
authors.initials: M
authors.lastname: Koyanagi
authors.forename: Asuka
authors.initials: A
authors.lastname: Morizane
authors.forename: Yo
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Horiguchi
authors.forename: Kei
authors.initials: K
authors.lastname: Tashiro
authors.forename: Tasuku
authors.initials: T
authors.lastname: Honjo
authors.forename: Shingo
authors.initials: S
authors.lastname: Fujii
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D006085:Graft Survival / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000187:drug effects* / Q000378:metabolism; D014183:Transplantation, Heterologous; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D004965:Estrogen Antagonists; D011960:Receptors, Estrogen; D004958:Estradiol; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine