Epicardial fiber organization in swine right ventricle and its impact on propagation.
Fiber organization is important for myocardial excitation and contraction. It can be a major factor in arrhythmogenesis and current distribution during defibrillation shocks. In this study, we report the discovery of a previously undetected thin epicardial layer in swine right ventricle (RV) with distinctly different fiber orientation, which significantly affects epicardial propagation. Experiments were conducted in isolated coronary-perfused right ventricular free wall preparations (n=8) stained with the voltage-sensitive dye di-4-ANEPPS. Optical signals were recorded from the epicardium with a CCD video camera at 800 fps. Preparations were sectioned parallel to the epicardial surface with a resolution of 50 mum or better. To link the histological data with the observed activation patterns, resulting fiber angles were introduced into a 3D computer model to simulate the electrical activation and voltage-dependent optical signals. In all preparations, we detected a thin epicardial layer with almost no depth-dependent fiber rotation. The thickness of this layer (z(0)) varied from 110 to 930 microm. At the boundary of this layer, we observed an abrupt change in fiber angle by 64+/-13 degrees followed by a gradual fiber rotation in the underlying layers. In preparations with z(0) <700 microm, optical mapping during epicardial stimulation revealed unusual diamond- and rectangular-shaped activation fronts with two axes of fast conduction. Computer simulations accurately predicted the features of the experimentally recorded activation fronts. The free wall of swine RV has a thin epicardial layer with distinctly different fiber orientation, which can significantly affect propagation and give rise to unusually shaped activation fronts. This is important for understanding electrical propagation in the heart, and further refines the existing knowledge of myocardial fiber architecture.
Vetter
Frederick J
FJ
Biomedical Engineering Program, Department of Electrical and Computer Engineering, University of Rhode Island, Kingston, USA.
Simons
Stephen B
SB
Mironov
Sergey
S
Hyatt
Christopher J
CJ
Pertsov
Arkady M
AM
D000200:Action Potentials; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology; D015687:Cell Hypoxia; D003198:Computer Simulation; D005456:Fluorescent Dyes / Q000032:analysis; D006329:Heart Conduction System / Q000502:physiology*; D006352:Heart Ventricles / Q000648:ultrastructure*; D021621:Imaging, Three-Dimensional; D008955:Models, Cardiovascular; D018485:Muscle Fibers, Skeletal / Q000648:ultrastructure*; D009200:Myocardial Contraction / Q000502:physiology*; D010496:Pericardium / Q000648:ultrastructure*; D011726:Pyridinium Compounds / Q000032:analysis; D012399:Rotation; D034421:Sus scrofa; D016276:Ventricular Function; D014741:Video Recording
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C050019:1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine