Protein damage in drop-on-demand printers.
Inkjet printing technology is used to synthesize microarrays consisting of a variety of compounds. In this communication, we characterize damage to a model enzyme, peroxidase, caused by the rapid compression experienced by the solution during the printing process. We also find that damage is mitigated by the addition of trehalose and glucose to the printed solution.
Nishioka
Gary M
GM
H & N Instruments, Inc., PO Box 4338, Newark, Ohio 43055, USA. HNI@Infinet.com
Markey
Andrea A
AA
Holloway
Charles K
CK
D004795:Enzyme Stability; D019793:Fluorescein / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D010544:Peroxidases / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*
D014199:Trehalose; D010544:Peroxidases; D005947:Glucose; D019793:Fluorescein