[Identification of the source and types of cells of Schistosoma japonicum by histochemical staining].
OBJECTIVE
To study the possibility on in vitro identifying the source or kinds of cells from Schistosoma japonicum (S.j).


METHODS
The cells from digested tissues of 18 days old schistosomula were smeared on slides. The adult worms of S.j were used for making paraffin sections. The cell smears and tissue sections were stained with 6 different methods of histochemical staining including Periodic Acid-Schiff (PAS), Argyrophil reaction (by Grimeliu's), Picric acid-acid Fuchsin (by Van Gieson, VG), Thionin, Toluidine blue (TB) and Hematoxylin-Eosin (HE) staining parallelly. The results were judged through inspecting the specific color of the cells on smears referring to location of corresponding staining of paraffin sections of adult worm tissues under light microscopy.


RESULTS
Vitelline gland cells, mother germ cells, nerve cells, digestive epithelial cells, muscular cells and mast cells were shown clearly. The stainings of VG, PAS and Thionin demonstrated cell types coinciding to histological location. The TB staining did not find red-purple mast cell in tissue sections but one in cell smears. The Grimeliu's argyrophil reaction displayed that intestine wall of tissure sections and stained cells of cell smears were in black clearly.


CONCLUSIONS
HE staining together with histochemical staining can reliably and rapidly distinguish the cell types of Schistosoma japonicum.
Lin
Xue-chi
XC
Department of Parasitology, Xiang-Ya School of Medicine, Central South University, Changsha 410078, China.
Zeng
Qing-ren
QR
Gong
Yan-fei
YF
Yan
Gan-wei
GW
Shen
Jie
J
D000818:Animals; D002478:Cells, Cultured; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D012549:Schistosoma japonicum / Q000166:cytology*