[Expression of the chosen proapoptotic and activation markers in adenoids in children with allergic rhinitis].
Naive T cells can become either tolerant or immune as a result of their first encounter with antigen. Apoptosis is the most important immunoregulatory mechanism leading to either regional tolerant or autoimmunity and allergy. The lymphatic structure closest to the nasal mucosa is the adenoid, the part of NALT - Nasal Associated Lymphoid Tissue. The objective of this study was to find evidence for our hypothesis that allergic sensitization can occur in adenoid. Of special interest the activity of proapoptotic markers of adenoids lymphocytes in interfollicular areas. In this study the number of cells presenting expression of chosen antigens in adenoids of 20 allergic and 20 non-allergic children were evaluated. The number of positive cell for CD3, CD4, CD8, CD25, CD28, CTLA4(CD152), Fas, FasL, bcl-2, bcl-x were determined using immunohistochemical staining techniques. The eosinophils in the interfollicular space showed a trend to be more numerous in the allergic group (p < 0.005). The number of CD4, CD28, FasL, CTLA-4 and bcl-x positive cells was higher in adenoids allergic than non-allergic children. The differences were statistically significant (p < 0.05; p < 0.001). The result of this study showed that the activity of pro-apoptotic and proliferation markers of the adenoid of allergic children was more intensive than in other children. These findings support that allergic sensitization either is related to or takes place in the adenoid.
Zakrzewska
Anna
A
Klinika Otolaryngologii, Audiologii i Foniatrii Dzieciecej IP UM w Lodzi.
Kobos
Józef
J
Gryczyńska
Danuta
D
D000234:Adenoids / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism; D017209:Apoptosis / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D060908:CTLA-4 Antigen; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000276:immunology / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology* / Q000378:metabolism*; D051020:bcl-X Protein
D015703:Antigens, CD; D000943:Antigens, Differentiation; C494811:BCL2L1 protein, human; D015415:Biomarkers; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein