title: Variable surface epitopes in the crystal structure of dengue virus type 3 envelope glycoprotein.
abstract: Dengue virus is an emerging global health threat. The major envelope glycoprotein, E, mediates viral attachment and entry by membrane fusion. Antibodies that bind but fail to neutralize noncognate serotypes enhance infection. We have determined the crystal structure of a soluble fragment of the envelope glycoprotein E from dengue virus type 3. The structure closely resembles those of E proteins from dengue type 2 and tick-borne encephalitis viruses. Serotype-specific neutralization escape mutants in dengue virus E proteins are all located on a surface of domain III, which has been implicated in receptor binding. While antibodies against epitopes in domain I are nonneutralizing in dengue virus, there are neutralizing antibodies that recognize serotype-conserved epitopes in domain II. The mechanism of neutralization for these antibodies is probably inhibition of membrane fusion. Our structure shows that neighboring glycans on the viral surface are spaced widely enough (at least 32 A) that they can interact with multiple carbohydrate recognition domains on oligomeric lectins such as DC-SIGN, ensuring maximum affinity for these putative receptors.
authors.affiliation: Children's Hospital, Enders 673, 320 Longwood Ave., Boston, MA 02115, USA.
authors.forename: Yorgo
authors.initials: Y
authors.lastname: Modis
authors.forename: Steven
authors.initials: S
authors.lastname: Ogata
authors.forename: David
authors.initials: D
authors.lastname: Clements
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Harrison
mesh_terms: D003460:Crystallization; D003716:Dengue Virus / Q000737:chemistry* / Q000145:classification; D019281:Dimerization; D000939:Epitopes; D011487:Protein Conformation; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D000939:Epitopes; D014759:Viral Envelope Proteins