title: Viscocanalostomy in rhesus monkeys.
abstract: OBJECTIVE
  To examine structural changes and aqueous humor outflow after viscocanalostomy in live normal monkey eyes.
  
  
  METHODS
  Viscocanalostomy surgery was performed in 1 eye of each of 4 rhesus monkeys. Outflow facility was determined before and after surgery. All eyes were fixed and examined by light and/or electron microscopy 36 or 63 days postoperatively.
  
  
  RESULTS
  Schlemm canal was replaced by scar tissue at the surgical site. The juxtacanalicular zone contained homogeneous material, probably high-molecular-weight 1.4% sodium hyaluronate. The sclera external to Schlemm canal was overhydrated, and remains of a scleral lake were present in 1 animal. Multiple defects were present in the endothelial lining of Schlemm canal inner and outer wall. Fine fibrillar material and sheath-derived plaque material partly bridged the defects. Along the inner wall, aggregations of thrombocytes covered some defects in the endothelial lining of the canal. At 90 degrees to 180 degrees from the surgical site, small and fewer breaks in the inner wall were seen. Postsurgery outflow facility (n = 2) was approximately 30% higher in the treated eye than in the contralateral control, corrected bilaterally for presurgery baseline.
  
  
  CONCLUSIONS
  The most likely explanations for the increase in outflow facility in monkeys after viscocanalostomy are focal disruptions of the inner wall endothelium of Schlemm canal and disorganization of the juxtacanalicular zone, resulting in direct communication of juxtacanalicular zone extracellular spaces with the lumen of Schlemm canal. The continuous presence of sodium hyaluronate might prevent repair of these defects by interfering with thrombocyte function.
  
  
  CONCLUSIONS
  In nonhuman primates, viscocanalostomy appears to decrease outflow resistance through persisting focal disruption of the inner wall endothelium and opening of the juxtacanalicular or cribriform region of the trabecular meshwork, the tissue most affected by pathologic changes in primary open-angle glaucoma in humans.
authors.affiliation: Department of Anatomy, Molecular Anatomy, and Embryology, University of Erlangen-Nürnberg, Germany.
authors.forename: Ernst R
authors.initials: ER
authors.lastname: Tamm
authors.forename: Roberto G
authors.initials: RG
authors.lastname: Carassa
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Albert
authors.forename: B'Ann T
authors.initials: BT
authors.lastname: Gabelt
authors.forename: Sarit
authors.initials: S
authors.lastname: Patel
authors.forename: Carol A
authors.initials: CA
authors.lastname: Rasmussen
authors.forename: Paul L
authors.initials: PL
authors.lastname: Kaufman
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects; D001082:Aqueous Humor / Q000378:metabolism; D001792:Blood Platelets / Q000648:ultrastructure; D002924:Ciliary Body / Q000648:ultrastructure; D004727:Endothelium / Q000648:ultrastructure; D005260:Female; D018463:Filtering Surgery / Q000379:methods*; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D007429:Intraocular Pressure; D007498:Iris / Q000648:ultrastructure; D008253:Macaca mulatta; D008297:Male; D014065:Tonometry, Ocular; D014129:Trabecular Meshwork / Q000378:metabolism / Q000601:surgery* / Q000648:ultrastructure
chemical_list: D006820:Hyaluronic Acid