[Chemiluminescence of the polymorphonuclear leukocytes-luminol system in the presence of biogenic chloramines].
It was demonstrated that N-chlorphenylalanine and other chloramines strengthen sharply chemiluminescence in the polymorphonuclear leukocytes (PML)-luminol system without special activation of cells. The intensity of chemiluminescence is higher than the intensity of luminol solution emission induced by N-chlorphenylalanine. But it was nearly equal to chemiluminescence intensity of a mixture of luminol, N-chlorphenylalanine and 20-30 nM H2O2. The increase in chemiluminescence in the PML-luminol system in the presence of N-chlorphenylalanine is not related to PML activation but is the result of direct oxidation of luminol by N-chlorphenylalanine. Chloramine derivatives of amino acids and taurine at final concentrations of 0.01-0.1 mM do not suppress luminol chemiluminescence in suspension of PML stimulated by phorbol-12-myristate-13-acetate. At the same time, hypochlorite inhibits sharply luminol emission induced by stimulated cells.
Murina
M A
MA
Belakina
N S
NS
Roshchupkin
D I
DI
D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002700:Chloramines / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006997:Hypochlorous Acid / Q000494:pharmacology; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D010703:Phorbol Esters / Q000494:pharmacology; D011817:Rabbits; D013329:Structure-Activity Relationship; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
D002700:Chloramines; D005343:Fibrinolytic Agents; D049408:Luminescent Agents; C454660:N,N-dichlorotaurine; C454659:N-chlorophenylalanine; D010703:Phorbol Esters; D013654:Taurine; D008165:Luminol; D006997:Hypochlorous Acid; C032974:12-O-retinoylphorbol-13-acetate; D006861:Hydrogen Peroxide; D000409:Alanine