Expression of interleukin-17B in mouse embryonic limb buds and regulation by BMP-7 and bFGF.
Interleukin-17B (IL-17B) is a member of interleukin-17 family that displays a variety of proinflammatory and immune modulatory activities. In this study, we found that IL-17B mRNA was maximally expressed in the limb buds of 14.5 days post coitus (dpc) mouse embryo and declined to low level at 19.5 dpc. By immunohistochemical staining, the strongest IL-17B signals were observed in the cells of the bone collar in the primary ossification center. The chondrocytes in the resting and proliferative zones were stained moderately, while little staining was seen in the hypertrophic zone. Furthermore, in both C3H10T1/2 and MC3T3-E1 cells, the IL-17B mRNA was up-regulated by recombinant human bone morphogenetic protein-7, but down-regulated by basic fibroblast growth factor via the extracellular signal-regulated kinase pathway. This study provides the first evidence that IL-17B is expressed in the mouse embryonic limb buds and may play a role in chondrogenesis and osteogenesis.
You
Zongbing
Z
Department of Orthopaedic Surgery, Center for Tissue Regeneration and Repair, School of Medicine, University of California, Davis, Sacramento, CA 95817, USA. zyou@ucdavis.edu
DuRaine
Grayson
G
Tien
Janet Y L
JY
Lee
Corinne
C
Moseley
Timothy A
TA
Reddi
A Hari
AH
D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology; D004622:Embryo, Mammalian / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D018878:Limb Buds; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D020381:Interleukin-17; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D048049:Extracellular Signal-Regulated MAP Kinases