Resistance of corynebacterial strains to infection and lysis by corynephage BFK 20.
OBJECTIVE
Defence mechanisms of the corynebacterial strains against corynephage BFK 20, which causes lysis of Brevibacterium flavum CCM 251.


RESULTS
We tested adsorption of the phage BFK 20 to the corynebacterial cell surface. We observed strong adsorption ranging from ca 79 to 93% on the cells of B. flavum ATCC strains, but only ca 76% for B. flavum CCM 251. Minor adsorption for Brevibacterium lactofermentum BLOB (ca 13%) and no adsorption for Corynebacterium glutamicum RM3 were determined. BFK 20 infection had no significant effect on growth and viability of C. glutamicum and B. lactofermentum, but significantly influenced growth and viability of B. flavum ATCC 21127, 21128 and 21474. Cell growth stopped in short time after infection but with no lysis. Brevibacterium flavum CCM 251 cell growth was arrested too and lysis occurred. The Southern hybridization confirmed the presence of significant amount of BFK 20 DNA in samples from B. flavum CCM 251 and B. flavum ATCC strains after BFK 20 infection. Only weak hybridization signal was detected for DNA from infected cells of B. lactofermentum BLOB and no signal for C. glutamicum RM3.


CONCLUSIONS
Based on the above results we suggest presence of a mechanism leading to abortive infection in B. flavum ATCC 21127, 21128 and 21474. In B. lactofermentum BLOB and C. glutamicum RM3 the adsorption barrier is more likely.


CONCLUSIONS
This study increases the knowledge on defence mechanisms of corynebacteria against bacteriophages.
Halgasová
N
N
Institute of Molecular Biology, Centre of Excellence for Molecular Medicine, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Majtán
T
T
Ugorcáková
J
J
Timko
J
J
Bukovská
G
G
D001431:Bacteriological Techniques; D001435:Bacteriophages / Q000502:physiology*; D001951:Brevibacterium / Q000821:virology; D040142:Brevibacterium flavum / Q000821:virology; D003352:Corynebacterium / Q000821:virology*; D008242:Lysogeny