[Study on DNA damage and expression of RAS gene in L-02 cells exposed to chlorinated drinking water extracts].
OBJECTIVE
To study the DNA damage and expression of RAS gene in human fetal hepatocytes (L-02) induced by chlorinated drinking water extracts, and to explore the possible molecular mechanism of its genotoxicity .


METHODS
L-02 cells were exposed to chlorinated drinking water extracts at the concentration of 0.167, 1.67, 16.7 and 167 ml/ml culture medium. Single cell gel electrophoresis (SCGE) and in situ hybridization (ISH) assay were used to detect DNA damage and the expression of RAS gene in L-02 cells. DMSO (10 ml/L) and B(a)P (200 micromol/L) were used as solvent control and positive control respectively.


RESULTS
Chlorinated drinking water extracts led to a significant increase of DNA migration in the L-02 cells at the concentrations of 16.7 and 167 ml/ml culture medium in comparison with solvent control. Significantly higher expressions of RAS gene was found in the L-02 cells exposed to all concentrations of chlorinated drinking water extracts than in that of solvent control group. There was a significant correlation (r = 0.99) between RAS gene expression and DNA migration when the concentration of chlorinated drinking water extracts was between 0.167 and 16.7 ml/ml.


CONCLUSIONS
Chlorinated drinking water extracts could induce obvious DNA damage and RAS gene expression in L-02 cells, and RAS gene expression might be affected by DNA damage.
Liu
Hui
H
Department of Occupational and Environmental Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Zou
Yaling
Y
Liu
Ailin
A
Li
Xiaoyan
X
Lu
Wenqing
W
D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D011905:Genes, ras; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D017403:In Situ Hybridization; D014881:Water Supply
D006843:Hydrocarbons, Chlorinated