Evaluation of circulating anti-Fasciola IgA antibodies by ELISA technique using two different Fasciola antigens.
Two types of Fasciola antigens: surface tegumental (ST) and excretory-secretory (ES) products of F. gigantica worms were used for performance of ELISA technique. Results showed that, the circulating anti-Fasciola IgA antibodies were detected in 98% and 94% and the specificity of the test was 93.4% and 89.7% using ST and ES antigens, respectively. As regards ST antigen, a highly significant elevation in the level of the circulating anti-Fasciola IgA antibodies was observed according to the intensity of infection as well as in those with hepatomegaly. There was a significant direct relation between the level of circulating anti-Fasciola IgA antibodies and eosinophilia, anaemia and liver function tests of fascioliasis patients when ST antigen was used.
Mattar
Mona A
MA
Department of Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
El-Toukhy
Hanan M
HM
D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D008297:Male; D008875:Middle Aged
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007070:Immunoglobulin A