title: The binding of MBL to common bacteria in infectious diseases of children.
abstract: OBJECTIVE
  To purify Mannan-binding lectin (MBL) from human serum and detect its binding ability to several kinds of bacteria common in infectious diseases of children.
  
  
  METHODS
  MBL was purified from human serum by affinity chromatography on mannan-Sepharose 4B column. Its binding ability to eight species, 97 strains of bacteria was detected by enzyme-linked lectin assay (ELLA).
  
  
  RESULTS
  MBL has different binding ability to bacteria and shows strong binding ability to Klebsiella ornithinolytica and Escherichia coli, but shows relatively lower binding ability to Staphylococcus haemolyticus, Enterobacter cloacae and Staphylococcus epidermidis. To different isolates of Klebsiella pneumoniae, Haemophilus influenzae and Staphylococcus aureus, MBL shows quite different binding ability.
  
  
  CONCLUSIONS
  MBL has different binding ability to different bacteria, and has relatively stronger binding ability to Gram-negative bacteria. Its binding ability to different isolates of certain kinds of bacteria is quite different.
authors.affiliation: Affiliated Children's Hospital, Zhejiang University, Hangzhou 310003, China. Shangsq33@mail.hz.zj.cn
authors.forename: Shi-qiang
authors.initials: SQ
authors.lastname: Shang
authors.forename: Guo-xian
authors.initials: GX
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Yu
authors.forename: Ke-Yi
authors.initials: KY
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003141:Communicable Diseases / Q000382:microbiology*; D006801:Humans; D037601:Mannose-Binding Lectin / Q000097:blood / Q000378:metabolism*; D011485:Protein Binding; D013045:Species Specificity
chemical_list: D037601:Mannose-Binding Lectin