title: Development and evaluation of a protein microarray chip for diagnosis of hepatitis C virus.
abstract: A protein chip diagnostic kit was developed for the diagnosis of hepatitis C virus (HCV) based on the protein chip technique and the immuno-concentration method. This kit was designed for low-density protein chips and also for the availability of multiple sample screening. Applicability of the chip was evaluated using 96 blood specimens and the results were compared to results of an anti-HCV enzyme immunoassay (EIA) test. With further development, the technology associated with the development of this chip could be applied to the simultaneous detection of multiple protein-protein, protein-ligand interactions.
authors.affiliation: Laboratory of Biochemistry, College of Life and Environmental Sciences, Korea University, Seoul 136-701, Korea.
authors.forename: Chul-Soo
authors.initials: CS
authors.lastname: Yuk
authors.forename: Hyung Ku
authors.initials: HK
authors.lastname: Lee
authors.forename: Hee Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Yong Keel
authors.initials: YK
authors.lastname: Choi
authors.forename: Byung Cheon
authors.initials: BC
authors.lastname: Lee
authors.forename: Bok-Hwan
authors.initials: BH
authors.lastname: Chun
authors.forename: Namhyun
authors.initials: N
authors.lastname: Chung
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006526:Hepatitis C / Q000097:blood* / Q000175:diagnosis*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D018937:Hepatitis C Antibodies