title: MultiPLX: automatic grouping and evaluation of PCR primers.
abstract: MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer-primer interactions, primer-product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs.
  
  
  BACKGROUND
  Binaries for Windows, Linux and Solaris are available from http://bioinfo.ebc.ee/download/. A graphical version with limited capabilities can be used through a web interface at http://bioinfo.ebc.ee/multiplx/. The source code of the program is available on request for academic users.
  
  
  BACKGROUND
  maido.remm@ut.ee.
authors.affiliation: Department of Bioinformatics, University of Tartu, Tartu, Estonia.
authors.forename: Lauris
authors.initials: L
authors.lastname: Kaplinski
authors.forename: Reidar
authors.initials: R
authors.lastname: Andreson
authors.forename: Tarmo
authors.initials: T
authors.lastname: Puurand
authors.forename: Maido
authors.initials: M
authors.lastname: Remm
mesh_terms: D000465:Algorithms; D017931:DNA Primers / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D010363:Pattern Recognition, Automated / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D012984:Software
chemical_list: D017931:DNA Primers