Crosstalk in the responses to abiotic and biotic stresses in Arabidopsis: analysis of gene expression in cytochrome P450 gene superfamily by cDNA microarray.
From Arabidopsis full-length cDNA libraries, we collected ca. 7000 (7K) independent full-length cDNAs to prepare a cDNA microarray. The 7K cDNA collection contains 49 cytochrome P450 genes. In this study, expression patterns of these cytochrome P450 genes were analyzed by a full-length cDNA microarray under various treatments, such as hormones (salicylic acid, jasmonic acid, ethylene, abscisic acid), pathogen-inoculation ( Alternaria brassicicola , Alternaria alternata ), paraquat, rose bengal, UV stress (UV-C), heavy metal stress (CuSO4), mechanical wounding, drought, high salinity and low temperature. Expression of 29 cytochrome P450 genes among them was induced by various treatments. Inoculation with A. brassicicola and A. alternata as biotic stresses increased transcript levels of 12 and 5 genes in Arabidopsis plants, respectively. In addition, some of the genes were also expressed by abiotic stresses. This suggests crosstalk between abiotic and biotic stresses. The promoter sequences and cis -acting elements of each gene were studied on the basis of full-length cDNA sequences. Most cytochrome P450 genes induced by both abiotic and biotic stresses contained the recognition sites of MYB and MYC, ACGT-core sequence, TGA-box and W-box for WRKY transcription factors in their promoters. These cis -acting elements are known to participate in the regulation of plant defense. The response of each gene to multiple stresses is strictly regulated.
Narusaka
Yoshihiro
Y
Department of Biology, Tokyo Gakugei University, 4-1-1 Nukuikita-machi, Koganei-shi, Japan.
Narusaka
Mari
M
Seki
Motoaki
M
Umezawa
Taishi
T
Ishida
Junko
J
Nakajima
Maiko
M
Enju
Akiko
A
Shinozaki
Kazuo
K
D000040:Abscisic Acid / Q000494:pharmacology; D000528:Alternaria / Q000254:growth & development; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D015152:Blotting, Northern; D020171:Botrytis / Q000254:growth & development; D016000:Cluster Analysis; D003517:Cyclopentanes / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D005810:Multigene Family / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D054883:Oxylipins; D010269:Paraquat / Q000494:pharmacology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D012395:Rose Bengal / Q000494:pharmacology; D020156:Salicylic Acid / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014466:Ultraviolet Rays
D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D017382:Reactive Oxygen Species; D012395:Rose Bengal; D012965:Sodium Chloride; C011006:jasmonic acid; D000040:Abscisic Acid; D003577:Cytochrome P-450 Enzyme System; C036216:ethylene; D020156:Salicylic Acid; D010269:Paraquat