Lignification in poplar plantlets fed with deuterium-labelled lignin precursors.
Lignification was investigated in wild-type (WT) and in transgenic poplar plantlets with a reduced caffeic acid O-methyl-transferase (COMT) activity. Coniferin and syringin, deuterated at their methoxyl, were incorporated into the culture medium of microcuttings. The gas chromatography-mass spectrometry (GC-MS) analysis of the thioacidolysis guaiacyl (G) and syringyl (S) lignin-derived monomers revealed that COMT deficiency altered stem lignification. GC-MS analysis proved that the deuterated precursors were incorporated into root lignins and, to a lower extent, in stem lignins without major effect on growth and lignification. Deuterium from coniferin was recovered in G and S lignin units, whereas deuterium from syringin was only found in S units, which further establishes that the conversion of G to S lignin precursors may occur at the level of p-OH cinnamyl alcohols.
Rolando
Christian
C
UFR de Chimie, UPRESA CNRS 8009 'Chimie organique et macromoléculaire', Université des sciences et technologies de Lille (Lille-l), bât C4, 59655 Villeneuve d'Ascq cedex, France.
Daubresse
Nicolas
N
Pollet
Brigitte
B
Jouanin
Lise
L
Lapierre
Catherine
C
D002934:Cinnamates / Q000008:administration & dosage*; D003903:Deuterium / Q000008:administration & dosage*; D005960:Glucosides / Q000008:administration & dosage*; D008031:Lignin / Q000502:physiology*; D010666:Phenylpropionates / Q000008:administration & dosage*; D018520:Plant Shoots / Q000502:physiology*; D032107:Populus / Q000502:physiology*
D002934:Cinnamates; D005960:Glucosides; D010666:Phenylpropionates; D008031:Lignin; D003903:Deuterium; C028305:syringin; C016316:coniferin