A novel selective medium for isolation of Streptococcus mutans.
The aim of this study was to improve the selective medium of Streptococcus mutans. A new selective medium, designated MS-MUTV, was prepared by adding 10 mg/l valinomycin to the MS-MUT medium previously described. The average recovery of S. mutans was 72.1%, and the growth of S. sobrinus and S. anginousus group was inhibited on MS-MUTV, but allowed on MS-MUT. One hundred and thirty-nine human saliva samples were examined and counted for S. mutans and non-S. mutans colonies. The recovery of S. mutans on MS-MUTV was similar to that on MS-MUT. Eighty-two and 7.9 percent of the saliva samples obtained S. mutans pure cultures, with no bacterial growth on MS-MUTV, respectively. The remaining 10.1% were contaminated with non-S. mutans, with low-level CFU. MS-MUTV is useful for the isolation of S. mutans alone from clinical samples in routine examinations.
Takada
K
K
Department of Microbiology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Hirasawa
M
M
D001431:Bacteriological Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media; D006801:Humans; D012463:Saliva / Q000382:microbiology*; D013295:Streptococcus mutans / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media