LC/MS characterization of meningococcal depolymerized polysaccharide group C reducing endgroup and internal repeating unit.
Hydrogen peroxide has been used to cleave the native Neisseria meningiditis polysaccharide (PS) from mega-Dalton molecular weight to a smaller size (approximately 20 kDa) depolymerized polysaccharide. The polysaccharide was examined after partial peroxide depolymerization to verify the presence of the carboxyl group at position 1 and the intactness of the internal sialic acid repeating units. The reducing end group of meningococcal polysaccharide type C was also examined after derivatization by L-tyrosine hydrazide. Partial peroxide depolymerization did not result in loss of the position 1 carboxyl group at the reducing end of the polysaccharide. In addition, no loss of structural integrity was noted for the internal sialic repeat units.
Cai
Xinxing
X
Analytical Science & Assay Development, Aventis Pasteur, Discovery Drive, Swiftwater, Pennsylvania 18370, USA.
Lei
Q Paula
QP
Lamb
David H
DH
Shannon
Anthony
A
Jacoby
Jason
J
Kruk
Joe
J
Kensinger
Richard D
RD
Ryall
Robert
R
Zablackis
Earl
E
Cash
Patricia
P
D002264:Carboxylic Acids / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000737:chemistry; D012794:Sialic Acids / Q000032:analysis; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D002264:Carboxylic Acids; D011108:Polymers; D011135:Polysaccharides, Bacterial; D012794:Sialic Acids; C037676:meningococcal group C polysaccharide; D014443:Tyrosine; D006861:Hydrogen Peroxide