Detection of aptamer-protein interactions using QCM and electrochemical indicator methods.
We report novel method of detection thrombin-aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity.
Hianik
Tibor
T
Faculty of Mathematics, Physics and Computer Sciences, Comenius University, 842 48 Bratislava, Slovakia. hianik@fmph.uniba.sk
Ostatná
Veronika
V
Zajacová
Zuzana
Z
Stoikova
Ekaterina
E
Evtugyn
Gennady
G
D052157:Aptamers, Nucleotide; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D008751:Methylene Blue / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D011791:Quartz; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis / Q000737:chemistry
D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; D011506:Proteins; C081676:thrombin aptamer; D011791:Quartz; D004247:DNA; D013917:Thrombin; D008751:Methylene Blue