The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP.
Leucine-rich repeat (LRR)-containing transmembrane receptor-like kinases (RLKs) are important components of plant signal transduction. The Arabidopsis thaliana somatic embryogenesis receptor-like kinase 1 (AtSERK1) is an LRR-RLK proposed to participate in a signal transduction cascade involved in embryo development. By yeast two-hybrid screening we identified AtCDC48, a homologue of the mammalian AAA-ATPase p97 and GF14lambda, a member of the Arabidopsis family of 14-3-3 proteins as AtSERK1 interactors. In vitro, the AtSERK1 kinase domain is able to transphosphorylate and bind both AtCDC48 and GF14lambda. In yeast, AtCDC48 interacts with GF14lambda and with the PP2C phosphatase KAPP. In plant protoplasts AtSERK1 interacts with GF14lambda.
Rienties
Ingrid M
IM
Laboratory of Biochemistry, Department of Agrotechnology and Food Sciences, Wageningen University and Research Centre, Dreijenlaan 3, 6703, HA, Wageningen, The Netherlands.
Vink
Josefien
J
Borst
Jan Willem
JW
Russinova
Eugenia
E
de Vries
Sacco C
SC
D048948:14-3-3 Proteins; D000251:Adenosine Triphosphatases; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000071636:Protein Phosphatase 2C; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000074405:Valosin Containing Protein
D048948:14-3-3 Proteins; C117576:AFT1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D011494:Protein Kinases; C440035:SERK1 protein, Arabidopsis; C090240:KAPP protein, Arabidopsis; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein