[Study of postmyocardial infarction scar-formation mechanisms: advantage of an experimental myocardial infarction model in mice].
Myocardial infarction involves scar-formation mechanisms in which inflammation, proliferation, cell differentiation, apoptosis and angiogenesis all play a role. Better knowledge of the scar-formation process would be helpful in developing new therapies. The authors have generated a mouse model for infarction because its possible application in transgenic mice would allow the role of target genes in postinfarction scar-formation mechanisms to be studied. An infarction is caused by ligating the descending branch of the left coronary artery. At various times after ligation, the mice are sacrificed to determine the size of the infarction, left ventricular function and the overall myocardial scar-formation process. Early mortality was 10%. Between the fourth and sixth day postsurgery, 25% of mice died of a ruptured, infarcted left ventricle. The size of the infarctions diminished with time, while the surface of the left ventricle increased. In hemodynamics, 15 and 30 days after infarction, left ventricle telediastolic pressure was higher, telesystolic pressure was lower and contractility in indexes had collapsed. After an inflammatory phase in which polynuclear neutrophils colonized the scar, granulation tissue set in with a proliferation of myofibroblasts and growth of new blood vessels. These cells disappeared from the scar gradually, leaving behind a matrix rich in collagen and devoid of any contractile properties. The authors have characterized a murine model of myocardial infarction, with applications in transgenic mice and in view of establishing new agents in postmyocardial infarction repair.
Barandon
Laurent
L
Institut de Cardiologie de Montréal, Montréal, Canada.
Couffinhal
Thierry
T
Dufourcq
Pascale
P
Daret
Danièle
D
Allières
Cécile
C
Alzieu
Philippe
P
Deville
Claude
C
Duplàa
Cécile
C
D000818:Animals; D002921:Cicatrix / Q000209:etiology / Q000473:pathology*; D003331:Coronary Vessels / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006342:Heart Rupture, Post-Infarction / Q000401:mortality / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000473:pathology / Q000601:surgery*; D011183:Postoperative Complications / Q000473:pathology; D012307:Risk Factors; D012680:Sensitivity and Specificity; D016019:Survival Analysis; D016277:Ventricular Function, Left / Q000502:physiology*; D020257:Ventricular Remodeling