title: Detection of changes in articular cartilage proteoglycan by T(1rho) magnetic resonance imaging.
abstract: The purpose of this work is to demonstrate the feasibility of T(1rho)-weighted magnetic resonance imaging (MRI) to quantitatively measure changes in proteoglycan content in cartilage. The T(1rho) MRI technique was implemented in an in vivo porcine animal model with rapidly induced cytokine-mediated cartilage degeneration. Six pigs were given an intra-articular injection of recombinant porcine interleukin-1beta (IL-1beta) into the knee joint before imaging to induce changes in cartilage via matrix metalloproteinase (MMP) induction. The induction of MMPs by IL-1 was used since it has been extensively studied in many systems and is known to create conditions that mimic in part characteristics similar to those of osteoarthritis. The contralateral knee joint was given a saline injection to serve as an internal control. T(1rho)-weighted MRI was performed on a 4 T whole-body clinical scanner employing a 2D fast spin-echo-based T(1rho) imaging sequence. T(1rho) relaxation parameter maps were computed from the T(1rho)-weighted image series. The average T(1rho) relaxation rate, R(1rho) (1/T(1rho)) of the IL-1beta-treated patellae was measured to be on average 25% lower than that of saline-injected patellae indicating a loss of proteoglycan. There was an average reduction of 49% in fixed charge density, measured via sodium MRI, of the IL-1beta-treated patellae relative to control corroborating the loss of proteoglycan. The effects of IL-1beta, primarily loss of PG, were confirmed by histological and immunochemical findings. The results from this study demonstrate that R(1rho) is able to track proteoglycan content in vivo.
authors.affiliation: B1 Stellar-Chance Laboratories, Department of Radiology, University of Pennsylvania, 422 Curie Blvd., Philadelphia, PA 19104-6100, USA. wheaton@seas.upenn.edu
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wheaton
authors.forename: George R
authors.initials: GR
authors.lastname: Dodge
authors.forename: Arijitt
authors.initials: A
authors.lastname: Borthakur
authors.forename: J Bruce
authors.initials: JB
authors.lastname: Kneeland
authors.forename: H Ralph
authors.initials: HR
authors.lastname: Schumacher
authors.forename: Ravinder
authors.initials: R
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry*; D017364:Collagenases / Q000032:analysis; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053509:Matrix Metalloproteinase 13; D023421:Models, Animal; D011509:Proteoglycans / Q000032:analysis*; D013552:Swine
chemical_list: D007375:Interleukin-1; D011509:Proteoglycans; D017364:Collagenases; D053509:Matrix Metalloproteinase 13