title: Inhibition of adherence of Mycobacterium avium complex and Mycobacterium tuberculosis to fibronectin on the respiratory mucosa.
abstract: Mycobacterium species adhere to the respiratory mucosa via mucus and fibronectin of extracellular matrix exposed by damaged epithelium. We have investigated whether inhibiting adherence to fibronectin influences subsequent infection of human respiratory tissue by Mycobacterium avium complex and Mycobacterium tuberculosis. Human respiratory tissue was pretreated with mycobacterial fibronectin attachment proteins prior to infection with M. avium complex and M. tuberculosis and the number of recoverable bacteria over time was compared to untreated controls. Inhibition significantly reduced recovery of M. avium complex at 15min (P= 0.02), 7days (P = 0.04), and 14 days (P= 0.03); whereas recovery of M. tuberculosis was only reduced at 15 min (P = 0.01) and not at later timepoints. We conclude that M. avium complex and M. tuberculosis infection of the mucosa proceeds by different mechanisms, since M. tuberculosis infection is independent of fibronectin adherence.
authors.affiliation: Royal Brompton Hospital, London SW3 6NP, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Middleton
authors.forename: M V
authors.initials: MV
authors.lastname: Chadwick
authors.forename: A G
authors.initials: AG
authors.lastname: Nicholson
authors.forename: A
authors.initials: A
authors.lastname: Dewar
authors.forename: R K
authors.initials: RK
authors.lastname: Groger
authors.forename: E J
authors.initials: EJ
authors.lastname: Brown
authors.forename: T L
authors.initials: TL
authors.lastname: Ratliff
authors.forename: R
authors.initials: R
authors.lastname: Wilson
mesh_terms: D018829:Adhesins, Bacterial / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D015269:Mycobacterium avium Complex / Q000302:isolation & purification / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification / Q000502:physiology*; D009924:Organ Culture Techniques; D020545:Respiratory Mucosa / Q000378:metabolism / Q000382:microbiology*
chemical_list: D018829:Adhesins, Bacterial; C117704:FAP-A protein, Mycobacterium avium; D005353:Fibronectins