Markers of oxidative stress in diabetic mothers and their infants during delivery.
Oxidative stress is probably a pathophysiological process leading to disadvantageous outcomes in diabetic pregnancies. We aimed to map a complex of potential markers of oxidative stress in this condition. Diabetic mothers had significantly higher concentrations of thiobarbituric acid reactive substances in the plasma [TBARS] both before (p<0.0001) and after (p<0.001) delivery and also their newborns showed higher values of TBARS (p<0.0001) in comparison with the control group. Diabetic mothers also showed lower concentrations of reduced glutathione in erythrocytes [GSH] both before (p<0.05) and after (p<0.01) delivery and their infants also had lower levels of GSH (p<0.0001). We found a lower total antioxidative capacity of plasma [AOC] before delivery (p<0.05) in the diabetic group in comparison with the control group. Newborns of diabetic mothers had higher plasmatic concentrations of apolipoproteine B [apo B] (p<0.05), higher erythrocyte glutathione peroxidase [GPx] activity (p<0.05) and lower pH (p<0.001) in the umbilical cord blood, when compared with infants of control non-diabetic mothers. We conclude that pregestational and gestational diabetes mellitus represent increased oxidative stress for both mother and her infant. TBARS in plasma are a valuable marker of oxidative stress in this condition. Disruption of glutathione peroxidase/glutathione pattern can be involved in pathophysiology of enhanced oxidative stress in diabetic pregnancies.
Rajdl
D
D
Institute of Clinical Biochemistry and Haematology, University Hospital and Charles University, Faculty of Medicine, Alej Svobody 80, 304 60 Pilsen, Czech Republic. rajdl@fnplzen.cz
Racek
J
J
Steinerová
A
A
Novotný
Z
Z
Stozický
F
F
Trefil
L
L
Siala
K
K
D000328:Adult; D001034:Apgar Score; D015415:Biomarkers; D002318:Cardiovascular Diseases / Q000503:physiopathology; D002585:Cesarean Section; D036861:Delivery, Obstetric; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000378:metabolism*; D016640:Diabetes, Gestational / Q000201:enzymology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D012307:Risk Factors; D012727:Sex Characteristics; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D015415:Biomarkers; D008074:Lipoproteins; D017392:Thiobarbituric Acid Reactive Substances