The V-ATPase inhibitors concanamycin A and bafilomycin A lead to Golgi swelling in tobacco BY-2 cells.
Concanamycin A and bafilomycin A are well-known inhibitors of V-ATPase activity. It is known that they interfere with intracellular protein trafficking in both animal and plant cells, but a cellular target for their action in plant cells has not been defined. Here we show that treatment with these inhibitors leads to a massive vacuolation of the Golgi apparatus. The effect is similar, but not identical, to that previously described for the Na+/K+ ionophores and is reversible after washing.
Robinson
D G
DG
Department Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, Heidelberg, Federal Republic of Germany. david.robinson@urz.uni-heidelberg.de
Albrecht
S
S
Moriysu
Y
Y
D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006056:Golgi Apparatus / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D018942:Macrolides / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology; D014026:Nicotiana / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014617:Vacuoles / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology
D004791:Enzyme Inhibitors; D018942:Macrolides; C057620:bafilomycin A; C036978:concanamycin A; D025262:Vacuolar Proton-Translocating ATPases