Feature genes of hepatitis B virus-positive hepatocellular carcinoma, established by its molecular discrimination approach using prediction analysis of microarray.
Recent introduction of a learning algorithm for cDNA microarray analysis has permitted to select feature set to accurately distinguish human cancers according to their pathological judgments. Here, we demonstrate that hepatitis B virus-positive hepatocellular carcinoma (HCC) could successfully be identified from non-tumor liver tissues by supervised learning analysis of gene expression profiling. Through learning and cross-validating HCC sample set, we could identify an optimized set of 44 genes to discriminate the status of HCC from non-tumor liver tissues. In an analysis of other blind-tested HCC sample sets, this feature set was found to be statistically significant, indicating the reproducibility of our molecular discrimination approach with the defined genes. One prominent finding was an asymmetrical distribution pattern of expression profiling in HCC, in which the number of down-regulated genes was greater than that of up-regulated genes. In conclusion, the present findings indicate that application of learning algorithm to HCC may establish a reliable feature set of genes to be useful for therapeutic target of HCC, and that the asymmetric expression pattern may emphasize the importance of suppressed genes in HCC.
Kim
Bu-Yeo
BY
Laboratory of Molecular Oncology, Korea Institute of Radiological and Medical Sciences, 215-4 Gongneung-Dong, Nowon-Ku, Seoul 139-706, Korea.
Lee
Je-Geun
JG
Park
Sunhoo
S
Ahn
Jae-Yeon
JY
Ju
Yeun-Jin
YJ
Chung
Jin-Haeng
JH
Han
Chul Ju
CJ
Jeong
Sook-Hyang
SH
Yeom
Young Il
YI
Kim
Sangsoo
S
Lee
Yong-Sung
YS
Kim
Chang-Min
CM
Eom
Eun-Mi
EM
Lee
Dong-Hee
DH
Choi
Kang-Yell
KY
Cho
Myung-Haing
MH
Suh
Kyung-Suk
KS
Choi
Dong-Wook
DW
Lee
Kee-Ho
KH
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000821:virology; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D015203:Reproducibility of Results