title: [Determination of epimedin C and icariin in Herba Epimedii by HPLC].
abstract: OBJECTIVE
  To establish a RP-HPLC method for the determination of epimedin C and icariin in Herba Epimedii.
  
  
  METHODS
  Chromatographic conditions: Hypersil BDS-C18 column, acetonitrice and water containing 0.05% phosphoric acid as gradient eluents, G1315A photodiode array detector at 272 nm.
  
  
  RESULTS
  The average recovery rate of epimedin C was 99.7%, RSD 1.5% (n = 9), icariin 102.5%, RSD 1.1% (n=9).
  
  
  CONCLUSIONS
  The method is reliable, stable and well repeatable. It provides the useful information for quality evaluation.
authors.affiliation: Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, Jiansu, China.
authors.forename: Ming-quan
authors.initials: MQ
authors.lastname: Wang
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Bi
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Ji
authors.forename: Fei-long
authors.initials: FL
authors.lastname: Cheng
mesh_terms: D002681:China; D004365:Drugs, Chinese Herbal / Q000032:analysis; D017753:Ecosystem; D034181:Epimedium / Q000737:chemistry* / Q000145:classification; D005419:Flavonoids / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013045:Species Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C106054:epimedin C; C056599:icariin