title: Perfusion of medium with supplemented growth factors changes metabolic activities and cell morphology of hepatocyte-nonparenchymal cell coculture.
abstract: To develop a feasible perfusion-type bioartificial liver device, perfusion of hepatocyte-nonparenchymal cell (NPC) cocultures with medium supplemented with hepatocyte growth factor (HGF) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) was carried out. On day 1 of culture, perfusion at a constant shear stress of 1.3 dyn/cm2 enhanced ammonia metabolic and urea synthetic activities of hepatocytes. These enhanced activities were sustained up to day 7 only when growth factors were present. In contrast, no beneficial effects of growth factors on these activities were observed in static cultures. In perfusion cultures, three-dimensional cell aggregates were formed. On the surface of these aggregates, flattened cell layers composed mainly of NPCs were found, and the central cluster of cell aggregates was composed of round-shaped hepatocytes and reticulin fibrils. These observations strongly suggested that the reconstruction of different types of liver cells and connective tissues formed tissue-mimicking cell aggregates in the perfusion culture that was able to modulate the liver-specific functions of hepatocytes. Thus, perfusion culture conditions of the hepatocyte--NPC coculture system should be appropriately designed to induce suitable reconstruction of the cultured cells for use as a bioartificial liver device.
authors.affiliation: Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8575, Japan.
authors.forename: Pei
authors.initials: P
authors.lastname: Kan
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Miyoshi
authors.forename: Norio
authors.initials: N
authors.lastname: Ohshima
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology; D066257:Heparin-binding EGF-like Growth Factor; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D019164:Liver, Artificial; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology; D008657:Metabolic Clearance Rate / Q000187:drug effects; D010477:Perfusion / Q000379:methods; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D014508:Urea / Q000378:metabolism
chemical_list: D000418:Albumins; D003470:Culture Media; D006133:Growth Substances; C587885:Hbegf protein, rat; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D000644:Quaternary Ammonium Compounds; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D014508:Urea