Improvement of nisin production in pH feed-back controlled, fed-batch culture by Lactococcus lactis subsp. lactis.
Nisin production by Lactococcus lactis subsp. lactis in fed-batch culture was doubled by using a pH feed-back controlled method. Sucrose concentration was controlled at 10 g l(-1) giving 5010 IU nisin ml(-1) compared to 2660 IU nisin ml(-1) in batch culture.
Lv
Wenhua
W
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, P.O. Box 353, Beijing, 100080, P.R. China.
Cong
Wei
W
Cai
Zhaoling
Z
D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D005246:Feedback / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D009561:Nisin / Q000096:biosynthesis*; D013395:Sucrose / Q000378:metabolism*
D009561:Nisin; D013395:Sucrose