Generation of clinical-grade monocyte-derived dendritic cells using the CliniMACS system.
We describe the generation of monocyte-derived dendritic cells (MoDC) from leukapheresis products using the CliniMACS system from Miltenyi Biotec. In a clinical setting, the method turned out to be feasible for the generation of clinical-grade MoDC from patients with metastatic renal-cell carcinoma. MoDC generated with this system exhibited a fully mature phenotype as well as high migratory and T-cell stimulatory capacity.
Putz
Thomas
T
Department of Urology, University of Innsbruck, Innsbruck, Austria.
Gander
Hubert
H
Ramoner
Reinhold
R
Zelle-Rieser
Claudia
C
Rahm
Andrea
A
Nussbaumer
Walter
W
Bartsch
Georg
G
Höltl
Lorenz
L
Thurnher
Martin
M
D002454:Cell Differentiation; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D007937:Leukapheresis; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000378:metabolism
C515370:CCR7 protein, human; D054400:Receptors, CCR7; D019707:Receptors, Chemokine