Characterization of HoMADS 1 and its induction by plant hormones during in vitro ovule development in Hyacinthus orientalis L.
To understand the molecular mechanism of ovule development, a MADS box gene, HoMADS 1 , has been isolated from the ovule tissues of Hyacinthus . Sequence comparison showed that HoMADS 1 is highly homologous to both class C and D genes. Furthermore, phylogenetic analysis suggests that HoMADS 1 is most likely a class D MADS box gene. RNA hybridization revealed that HoMADS 1 was exclusively expressed in the ovules. Over-expressing HoMADS 1 in transgenic Arabidopsis plants produced ectopic carpelloid structures, including ovules, indicating that HoMADS 1 is involved in the determination of carpel and ovule identities. Interestingly, during in vitro flowering, no HoMADS 1 mRNA was detected in the floral tissues at high level hormones in the media. However, HoMADS 1 mRNA accumulated in the floral tissues when the regenerated flowers were transferred to the media containing low level hormones which could induce in vitro ovule formation. Our data suggest that the induction of HoMADS 1 by plant hormones may play important roles during ovule initiation and development in the regenerated flower. Whether HoMADS 1 expression is also regulated by cytokinin and auxin during ovule development in planta remains to be investigated.
Xu
Hong Yan
HY
College of Life Sciences, Shandong Agricultural University, Taian, Shandong, China.
Li
Xing Guo
XG
Li
Quan Zi
QZ
Bai
Shu Nong
SN
Lu
Wen Liang
WL
Zhang
Xian Sheng
XS
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D003583:Cytokinins / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D031423:Hyacinthus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D007210:Indoleacetic Acids / Q000494:pharmacology; D026161:MADS Domain Proteins / Q000235:genetics*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D046509:Tissue Culture Techniques
D003583:Cytokinins; D018076:DNA, Complementary; D007210:Indoleacetic Acids; D026161:MADS Domain Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger