Diverse human aldolase C gene promoter regions are required to direct specific LacZ expression in the hippocampus and Purkinje cells of transgenic mice.
Aldolase C is selectively expressed in the hippocampus and Purkinje cells in adult mammalian brain. The gene promoter regions governing cell-specific aldolase C expression are obscure. We show that aldolase C messenger expression in the hippocampus is restricted to CA3 neurons. The human distal promoter region (-200/-1200 bp) is essential for beta-galactosidase (beta-gal) expression in CA3 neurons and drives high stripe-like beta-gal expression in Purkinje cells. The 200 bp proximal promoter region is sufficient to drive low brain-specific and stripe-like beta-gal expression in Purkinje cells. Thus, the human aldolase C gene sequences studied drive endogenous-like expression in the brain.
Buono
Pasqualina
P
Facoltà di Scienze Motorie, Università Parthenope, Naples, Italy.
Barbieri
Ottavia
O
Alfieri
Andreina
A
Rosica
Annamaria
A
Astigiano
Simonetta
S
Cantatore
Daniela
D
Mancini
Annamaria
A
Fattoruso
Olimpia
O
Salvatore
Francesco
F
D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D009474:Neurons / Q000201:enzymology; D011401:Promoter Regions, Genetic; D011689:Purkinje Cells / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D007527:Isoenzymes; D012333:RNA, Messenger; D001616:beta-Galactosidase; D005634:Fructose-Bisphosphate Aldolase