title: Technical aspects of an impact acceleration traumatic brain injury rat model with potential suitability for both microdialysis and PtiO2 monitoring.
abstract: This report describes technical adaptations of a traumatic brain injury (TBI) model-largely inspired by Marmarou-in order to monitor microdialysis data and PtiO2 (brain tissue oxygen) before, during and after injury. We particularly focalize on our model requirements which allows us to re-create some drastic pathological characteristics experienced by severely head-injured patients: impact on a closed skull, no ventilation immediately after impact, presence of diffuse axonal injuries and secondary brain insults from systemic origin... We notably give priority to minimize anaesthesia duration in order to tend to banish any neuroprotection. Our new model will henceforth allow a better understanding of neurochemical and biochemical alterations resulting from traumatic brain injury, using microdialysis and PtiO2 techniques already monitored in our Intensive Care Unit. Studies on efficiency and therapeutic window of neuroprotective pharmacological molecules are now conceivable to ameliorate severe head-injury treatment.
authors.affiliation: Department of Neurochemistry, I.M.N.S.S.A., B.P. 610, 83800 Toulon Armées, France. emilie_carre@hotmail.com
authors.forename: Emilie
authors.initials: E
authors.lastname: Carré
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Cantais
authors.forename: Olivier
authors.initials: O
authors.lastname: Darbin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Terrier
authors.forename: Michel
authors.initials: M
authors.lastname: Lonjon
authors.forename: Bruno
authors.initials: B
authors.lastname: Palmier
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Risso
mesh_terms: D000054:Acceleration / Q000009:adverse effects*; D018681:Anesthetics, General / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology; D001930:Brain Injuries / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D020833:Diffuse Axonal Injury / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D045604:Extracellular Fluid / Q000378:metabolism; D016489:Head Injuries, Closed / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D017551:Microdialysis; D009410:Nerve Degeneration / Q000473:pathology / Q000503:physiopathology; D018696:Neuroprotective Agents / Q000494:pharmacology; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018681:Anesthetics, General; D018696:Neuroprotective Agents