title: Human peripheral and gastric lymphocyte responses to Helicobacter pylori NapA and AphC differ in infected and uninfected individuals.
abstract: BACKGROUND
  In this study, we identify the nature of the immunological response of human peripheral blood mononuclear cells (PBMC) and lamina propria gastric lymphocytes (LPL) to two Helicobacter pylori antigens, the neutrophil activating protein (NapA) and alkyl hydroperoxide reductase (AphC). These antigens were identified and selected for study based on the observation that serological recognition of these proteins was associated with H pylori negative status in humans.
  
  
  OBJECTIVE
  The aim was to study the serological, proliferative, and cytokine responses of PBMC and LPL, obtained from H pylori infected and uninfected individuals, to these antigens.
  
  
  METHODS
  Patient serum, PBMC, and LPL were used to determine antibody isotype, and proliferative and cytokine responses to recombinant forms of NapA and AphC using western blotting and ELISA.
  
  
  RESULTS
  Western blotting revealed antibody reactivity to recombinant NapA and AphC among the H pylori negative population studied. Both the proliferative and interferon gamma responses of PBMC and LPL to NapA and AphC were significantly higher in H pylori negative compared with H pylori positive subjects. Analysis of the IgG subclass profiles to both antigens revealed a T helper 1 associated IgG3 antibody response in uninfected individuals. However, interleukin 10 production was greater in H pylori positive individuals in response to these antigens.
  
  
  CONCLUSIONS
  Taken together these data are consistent with an immune response to these antigens skewed towards a T helper 1 response in the uninfected cohort.
authors.affiliation: Trinity Centre for Health Sciences, Department of Clinical Medicine, St James's Hospital, Dublin 8, Ireland. hjwindle@tcd.ie
authors.forename: H J
authors.initials: HJ
authors.lastname: Windle
authors.forename: Y S
authors.initials: YS
authors.lastname: Ang
authors.forename: V
authors.initials: V
authors.lastname: Athie-Morales
authors.forename: V A
authors.initials: VA
authors.lastname: Morales
authors.forename: R
authors.initials: R
authors.lastname: McManus
authors.forename: D
authors.initials: D
authors.lastname: Kelleher
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010544:Peroxidases / Q000276:immunology*; D054464:Peroxiredoxins; D011994:Recombinant Proteins / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori; D016753:Interleukin-10; D007371:Interferon-gamma; D010544:Peroxidases; D054464:Peroxiredoxins