title: Comparison of cytomegalovirus pp-65 antigenemia assay and plasma DNA correlation with the clinical outcome in transplant recipients.
abstract: The relationship between quantitative antigenemia and plasma DNAemia was studied for monitoring cytomegalovirus (CMV) viremia in CMV infection (CMVI) or disease (CMVD), in 20 transplant recipients (13 CMD, seven CMVI). A total of 142 samples of blood were assayed for CMV-DNA and pp-65 antigenemia (CMV-Ag). A quantitative correlation between both markers was found (P < 0.0001). First CMV antigenemia as well as first plasma DNA viral load was similar in CMVI and CMVD (29 vs. 24 CMV-Ag+ cells/10(5) PMN; and 7445 vs. 12407 CMV-DNA copies/ml). The maximum antigenemia was higher in CMVD than in CMVI (146 +/- 87 vs. 61 +/- 54 +cells/10(5) PMN, P < 0.05), but the highest CMV plasma viral load was similar in both groups (62592 +/- 33000 vs. 42055 +/- 38600 copies/ml). In nine patients, maximum antigenemia coincided with highest plasma DNA viral load, but in 10 highest DNAemia occurred 6 days later. On the contrary, antigenemia declined faster than CMV-DNAnemia, after treatment.
authors.affiliation: Services of Microbiology, HUCA, 33006 Oviedo, Asturias, Spain. maria.ona@sespa.princast.es
authors.forename: María
authors.initials: M
authors.lastname: de Oña
authors.forename: Santiago
authors.initials: S
authors.lastname: Melón
authors.forename: María C
authors.initials: MC
authors.lastname: Galarraga
authors.forename: Ana
authors.initials: A
authors.lastname: Palacio
authors.forename: José L
authors.initials: JL
authors.lastname: Lambert
authors.forename: María J
authors.initials: MJ
authors.lastname: Bernardo
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Ernesto
authors.initials: E
authors.lastname: Gómez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000097:blood* / Q000276:immunology; D004279:DNA, Viral / Q000097:blood* / Q000302:isolation & purification; D016027:Heart Transplantation / Q000009:adverse effects; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D016031:Liver Transplantation / Q000009:adverse effects*; D008875:Middle Aged; D010750:Phosphoproteins / Q000097:blood*; D016896:Treatment Outcome; D019562:Viral Load; D014763:Viral Matrix Proteins / Q000097:blood*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa