Sequential injection spectrophotometric determination of etilefrine hydrochloride.
A simple, fast, economical and automated sequential injection spectrophotometric method for the determination of etilefrine hydrochloride is developed. The method is based on the condensation reaction of etilefrine hydrochloride with 4-aminoantipyrine in the presence of alkaline potassium hexacyanoferrate and the absorbance of the colored product measured at 503 nm. Aspiration order, flow rate, reaction coil diameter, reaction coil length, concentration of 4-aminoantipyrine and potassium ferricyanide, as well as aspiration volume of reagents and sample has been optimized. Using these optimized parameters, a linear relationship between the relative peak height and concentration was obtained in the range 1-20 mg l(-1). The detection limit (as 3sigma value) was 0.1 mg l(-1) and precision was 2.7% and 1.5% at 1 and 2 mg l(-1), respectively. This method is superior over previously reported ones in terms of linear range, short analysis time, high sample throughput, excellent reagent economy and minimum waste generation.
Beyene
Negussie W
NW
Department of Chemistry, University of Pretoria, 0002 Pretoria, South Africa.
van Staden
Jacobus F
JF
Stefan
Raluca I
RI
D005039:Etilefrine / Q000032:analysis* / Q000737:chemistry; D017022:Flow Injection Analysis / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
D005039:Etilefrine