[Comparison studies on epidermis cell culture efficiency between big graft method and small strip method].
OBJECTIVE
To compare the efficiency of epidermis cell culture between big graft method and small strip method.


METHODS
The big graft method was to cut the skin tissue reticularly from dermis layer while the epidermis were not cut off. After it was digested fully in trypsin, the epidermis was separated from skin and was used to culture epidermal cells. The small strip method was routine. The time to cut the skin and to separate the epidermis was recorded, and the number and quality of cells were compared between two methods.


RESULTS
It took 8-10 minutes to cut an area of 5 cm2 skin into small strips and 1-2 minutes into big grafts. It took 10-15 minutes to separate the epidermis from the same area skin by small strip method and 2 minutes by big graft method. The cells showed better vigor and its number was more by big grafts than by small strips. The chance of fibroblast contamination was reduced obviously.


CONCLUSIONS
The big graft method is simpler than the small strip method and can culture more epidermis cells with less chance of fibroblast contamination.
Lu
Tao
T
The Dermatology Center of the Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an Shaanxi, PR China. lutao@fmmu.edu.cn
Jin
Yan
Y
Gao
Tianwen
T
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D000078404:Epidermal Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL