Fibrin as a cell carrier in cardiovascular tissue engineering applications.
In cardiovascular tissue engineering approaches, efficient seeding methods are essential. To achieve this and to save time, cells can be encapsulated in gels. Combining the advantages of a gel as a cell carrier with the advantages of a fiber-based scaffold, providing structural integrity to the developing tissue, might offer several advantages. In this study, seeding by using fibrin as a cell carrier is compared to the conventional static seeding method with regard to tissue development. Seeding with fibrin resulted in less loss of soluble collagen into the medium and a more mature extracellular matrix in a shorter period of time. The use of fibrin degradation inhibitors was shown to inhibit extracellular matrix formation, although it did not hamper cell proliferation. The use of fibrin as a cell carrier to seed cells into a fiber-based scaffold may represent a promising, timesaving approach in cardiovascular tissue engineering applications.
Mol
Anita
A
Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands. a.mol@tue.nl
van Lieshout
Marjolein I
MI
Dam-de Veen
Christa G
CG
Neuenschwander
Stefan
S
Hoerstrup
Simon P
SP
Baaijens
Frank P T
FP
Bouten
Carlijn V C
CV
D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D001698:Biomedical Engineering; D002319:Cardiovascular System / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D003470:Culture Media / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005337:Fibrin / Q000737:chemistry* / Q000378:metabolism; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D006909:Hydroxyproline / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D012501:Saphenous Vein / Q000166:cytology; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D003470:Culture Media; D005337:Fibrin; D003094:Collagen; D004247:DNA; D006909:Hydroxyproline