title: Quantitative analysis of tumor mitochondrial RNA using microarray.
abstract: OBJECTIVE
  To design a novel method to rapidly detect the quantitative alteration of mtRNA in patients with tumors.
  
  
  METHODS
  Oligo 6.22 and Primer Premier 5.0 bio-soft were used to design 15 pairs of primers of mtRNA cDNA probes in light of the functional and structural property of mtDNA, and then RT-PCR amplification was used to produce 15 probes of mtRNA from one normal gastric mucosal tissue. Total RNA extracted from 9 gastric cancers and corresponding normal gastric mucosal tissues was reverse transcribed into cDNA labeled with fluorescein. The spotted mtDNA microarrays were made and hybridized. Finally, the microarrays were scanned with a GeneTAC laser scanner to get the hybridized results. Northern blot was used to confirm the microarray results.
  
  
  RESULTS
  The hybridized spots were distinct with clear and consistent backgrounds. After data was standardized according to the housekeeping genes, the results showed that the expression levels of some mitochondrial genes in gastric carcinoma were different from those in the corresponding non-cancerous regions.
  
  
  CONCLUSIONS
  The mtDNA expression microarray can rapidly, massively and exactly detect the quantity of mtRNA in tissues and cells. In addition, the whole expressive information of mtRNA from a tumor patient on just one slide can be obtained using this method, providing an effective method to investigate the relationship between mtDNA expression and tumorigenesis.
authors.affiliation: Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China. hancb@126.com
authors.forename: Cheng-Bo
authors.initials: CB
authors.lastname: Han
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Mao
authors.forename: Yan
authors.initials: Y
authors.lastname: Xin
authors.forename: Shao-Cheng
authors.initials: SC
authors.lastname: Wang
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Ma
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Zhao
mesh_terms: D015152:Blotting, Northern; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D000077278:RNA, Mitochondrial; D012334:RNA, Neoplasm; D012313:RNA