Gene expression profile of an adenomyoepithelioma of the breast with a reciprocal translocation involving chromosomes 8 and 16.
Myoepithelium is an integral part of the mammary ductal and lobular architecture, positioned between luminal cells and the basement membrane. We describe the first report on cytogenetic findings in an adenomyoepithelioma of the breast with a balanced t(8;16)(p23;q21), and provide gene expression profile using Affymetrix GeneChip U95AV2 (Affymetrix, Santa Clara, CA). Differential analysis identified 857 genes with 2-fold or more mRNA change in comparison to pooled normal breast control; immunohistochemical analysis was used to confirm these results in a limited number of genes. Expression results were grouped based on the chromosomal location of the genes and associated protein function, and identified several potential pathogenetic mechanisms (autocrine and paracrine growth stimuli) in the development of myoepithelial tumors.
Gatalica
Zoran
Z
Department of Pathology, Creighton University School of Medicine, 601 North 30th Street, Omaha, NE 68131-2197. zgatalica@pathology.creighton.edu
Velagaleti
Gopalrao
G
Kuivaniemi
Helena
H
Tromp
Gerard
G
Palazzo
Juan
J
Graves
Kerry M
KM
Guigneaux
Michelle
M
Wood
Thomas
T
Sinha
Mala
M
Luxon
Bruce
B
D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16; D002898:Chromosomes, Human, Pair 8; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D009208:Myoepithelioma / Q000235:genetics*; D014178:Translocation, Genetic