title: Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells.
abstract: Myeloid-related protein 8 (MRP8) and MRP14, S100 proteins secreted by activated phagocytes, bind specifically to endothelial cells. The endothelial response to MRP8/MRP14, however, is unknown. Using oligonucleotide microarray analysis, we show for the first time that MRP8/MRP14 induce a thrombogenic, inflammatory response in human microvascular endothelial cells by increasing the transcription of proinflammatory chemokines and adhesion molecules and by decreasing the expression of cell junction proteins and molecules involved in monolayer integrity. All changes on the gene expression level could be confirmed using biochemical and functional assays. We demonstrated that the expression of MRP8/MRP14 closely correlated with the inflammatory activity in systemic vasculitis, confirming the important role of these proteins for distinct inflammatory reactions in endothelia. MRP8/MRP14 may represent novel targets for anti-inflammatory strategies.
authors.affiliation: Institute of Experimental Dermatology, Integrated Functional Genomics, and the Interdisciplinary Clinical Research Center, University Hospital Muenster, University of Muenster, Röntgenstrasse 21, 48149 Muenster, Germany. viemannd@uni-muenster.de
authors.forename: Dorothee
authors.initials: D
authors.lastname: Viemann
authors.forename: Anke
authors.initials: A
authors.lastname: Strey
authors.forename: Annette
authors.initials: A
authors.lastname: Janning
authors.forename: Kerstin
authors.initials: K
authors.lastname: Jurk
authors.forename: Kerstin
authors.initials: K
authors.lastname: Klimmek
authors.forename: Thomas
authors.initials: T
authors.lastname: Vogl
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hirono
authors.forename: Fukiko
authors.initials: F
authors.lastname: Ichida
authors.forename: Dirk
authors.initials: D
authors.lastname: Foell
authors.forename: Beate
authors.initials: B
authors.lastname: Kehrel
authors.forename: Volker
authors.initials: V
authors.lastname: Gerke
authors.forename: Clemens
authors.initials: C
authors.lastname: Sorg
authors.forename: Johannes
authors.initials: J
authors.lastname: Roth
mesh_terms: D040501:Calgranulin A / Q000235:genetics / Q000276:immunology*; D040502:Calgranulin B / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D002196:Capillaries / Q000166:cytology / Q000276:immunology; D002199:Capillary Permeability / Q000276:immunology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D007365:Intercellular Junctions / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D013927:Thrombosis / Q000276:immunology; D014657:Vasculitis / Q000276:immunology* / Q000503:physiopathology*
chemical_list: D040501:Calgranulin A; D040502:Calgranulin B; D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1