Nonhepatic cell lines HeLa and 293 support efficient replication of the hepatitis C virus genotype 2a subgenomic replicon.
The hepatitis C virus (HCV) genotype 2a subgenomic replicon can replicate in two human non-hepatocyte-derived cell lines, HeLa and 293, with in vitro-transcribed replicon RNA. Sequencing analysis revealed that mutations in HCV-derived regions were not essential for replication in these cells, as some clones displayed no mutations.
Kato
Takanobu
T
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan.
Date
Tomoko
T
Miyamoto
Michiko
M
Zhao
Zijiang
Z
Mizokami
Masashi
M
Wakita
Takaji
T
D002460:Cell Line; D016679:Genome, Viral; D006367:HeLa Cells; D016174:Hepacivirus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D009154:Mutation; D012093:Replicon / Q000235:genetics*; D014779:Virus Replication