title: Glycogen- and PP1c-targeting subunit GM is phosphorylated at Ser48 by sarcoplasmic reticulum-bound Ca2+-calmodulin protein kinase in rabbit fast twitch skeletal muscle.
abstract: Multifunctional Ca(2+)-calmodulin-dependent protein kinase (CaMKII) is a Ser/Thr protein kinase uniformly distributed within the sarcoplasmic reticulum (SR) of skeletal muscle. In fast twitch muscle, no specific substrates of CaMKII have yet been identified in nonjunctional SR. Previous electron microscopy data showed that glycogen particles containing glycogen synthase (GS) associate with SR at the I band level. Furthermore, recent evidence implicates CaMKII in regulation of glucose and glycogen metabolism. Here, we demonstrate that the glycogen- and protein phosphatase 1-targeting subunit, also known as G(M), selectively localizes to the SR membranes of rabbit skeletal muscle and that G(M) and GS co-localize at the level of the I band. We further show that G(M), GS, and PP1c assemble in a structural complex that selectively localizes to nonjunctional SR and that G(M) is phosphorylated by SR-bound CaMKII and dephosphorylated by PP1c. On the other hand, no evidence for a structural interaction between G(M) and CaMKII was obtained. Using His-tagged G(M) recombinant fragments and site-directed mutagenesis, we demonstrate that the target of CaMKII is Ser(48). Taken together, these data suggest that SR-bound CaMKII participates in the regulation of GS activity through changes in the phosphorylation state of G(M). Based on these findings, we propose that SR-bound CaMKII participates in the regulation of glycogen metabolism, under physiological conditions involving repetitive raises elevations of [Ca(2+)](i).
authors.affiliation: Department of Experimental Biomedical Sciences, University of Padova, viale Giuseppe Colombo 3, 35121 Padova, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Sacchetto
authors.forename: Elisa
authors.initials: E
authors.lastname: Bovo
authors.forename: Arianna
authors.initials: A
authors.lastname: Donella-Deana
authors.forename: Ernesto
authors.initials: E
authors.lastname: Damiani
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D046912:Multiprotein Complexes / Q000096:biosynthesis; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D054645:Protein Phosphatase 1; D021122:Protein Subunits / Q000378:metabolism; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000737:chemistry* / Q000378:metabolism; D012694:Serine / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D012694:Serine; D006003:Glycogen; D006006:Glycogen Synthase; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1