The angiogenic factors CXCL8 and VEGF in breast cancer: regulation by an array of pro-malignancy factors.
The regulation of secretion of the angiogenic factors CXCL8 and Vascular Endothelial Growth Factors (VEGF) was determined in breast tumor cells and in monocytic cells (as host cells that contribute to breast cancer). CXCL8 secretion, and partly the secretion of VEGF, were up-regulated in monocytic cells, but not in breast tumor cells, by the CC chemokines CCL5 and CCL2. EGF potently up-regulated CXCL8 secretion by breast tumor cells, and its effect was promoted by a consecutive treatment of the cells by estrogen and progesterone. These findings provide evidence for a complex set of pro-malignancy factors that may control the expression of angiogenic mediators at breast tumor sites.
Azenshtein
Elina
E
Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel.
Meshel
Tsipi
T
Shina
Sima
S
Barak
Nely
N
Keydar
Iafa
I
Ben-Baruch
Adit
A
D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000494:pharmacology; D018946:Chemokine CCL5; D019742:Chemokines, CC / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects* / Q000378:metabolism
C111835:CCL2 protein, human; C483024:CCL5 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D019742:Chemokines, CC; D004967:Estrogens; D016209:Interleukin-8; D042461:Vascular Endothelial Growth Factor A; D011374:Progesterone; D004815:Epidermal Growth Factor