Eugenol as an in vivo radioprotective agent.
In the present work, an attempt has been made to evaluate the possible in vivo radioprotection by eugenol. Swiss albino mice were administered different doses of eugenol (75,150 and 300 mg/kg) before exposure to 1.5 Gy of gamma radiation. The micronucleus test was carried out to determine the genetic damage in bone marrow. Our results demonstrated significant reduction in the frequencies of micronucleated polychromatic erythrocytes (MnPCEs) with all three eugenol doses. Eugenol (150 mg/kg) was also tested against different doses of radiation (0.5, 1, 1.5, and 2 Gy) and was found to afford significant radioprotection. Reduction in the incidence of MnPCEs could be noticed up to 72 h postirradiation (1.5 Gy). Moreover, the level of peroxidative damage and the specific activities of lactate dehydrogenase (LDH) and methylglyoxalase I (Gly I) were observed in the liver of mice treated with eugenol for seven days in comparison to untreated mice. The results revealed that eugenol exerted significant protection against oxidative stress. This possibility was further supported by the enhanced response of Gly I and the lowered activity of LDH. The present findings suggested that eugenol has a radioprotective potential.
Tiku
Ashu Bhan
AB
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Abraham
Suresh K
SK
Kale
Raosaheb K
RK
D000818:Animals; D001853:Bone Marrow / Q000502:physiology / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D005054:Eugenol / Q000008:administration & dosage / Q000494:pharmacology*; D005720:Gamma Rays; D005796:Genes / Q000528:radiation effects*; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*
D011837:Radiation-Protective Agents; D005054:Eugenol