title: [Amplification and sequencing of the gene coding for mucin-like protein from Schistosoma japonicum].
abstract: OBJECTIVE
  To amplify and sequence the partial gene coding for mucin-like protein from Chinese isolates of Schistosoma japonicum (SjMLP).
  
  
  METHODS
  The antigenic determinants of SmMLP were predicted by PCGENE software and specific oligonucleotide primers were designed and synthesized. Total RNA was isolated from adult worms of S. japonicum using Trizol reagent and the coding region gene of SjMLP was amplified by RT-PCR technique.
  
  
  RESULTS
  The coding region of SjMLP gene was specifically amplified by RT-PCR and the size of amplified fragment was 756 base pairs. The DNA sequence analysis result indicated that the coding sequence of the MLP was highly homologous between S. mansoni and S. japonicum.
  
  
  CONCLUSIONS
  The amplified fragment is consistent to the predicted one, providing a basis for cloning and further study on DNA immunization.
authors.affiliation: Department of Parasitology, College of Basic Medicine, Nanhua University, Hengyang 421001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Xiao
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.forename: Gu-qing
authors.initials: GQ
authors.lastname: Zeng
authors.forename: Yu-kuai
authors.initials: YK
authors.lastname: Zhang
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019444:Vaccines, DNA / Q000235:genetics
chemical_list: D000947:Antigens, Helminth; D009077:Mucins; D011506:Proteins; D019444:Vaccines, DNA