Rhythmic changes in spike coding in the rat suprachiasmatic nucleus.
The suprachiasmatic nucleus is regarded as the main mammalian circadian pacemaker but evidence for rhythmic firing of single units in vivo has been obtained only recently. The present study was undertaken to determine if rhythms could be seen using measures of activity in addition to the mean spike frequency. We investigated whether there were changes in the irregularity of cell activity measured by the disorder of the interspike interval distribution for neurones recorded in vivo and in vitro. By plotting the entropy of the log interval histogram that quantifies the coding capacity for each action potential against the respective zeitgeber time, we describe oscillations of spike activity in vivo. Entropy measures have the advantage over variances in that they quantify aspects of the shape of the distribution and not just the dispersion. One hundred and sixty-six cell recordings from the suprachiasmatic nucleus showed a significant rhythm in entropy with an oscillatory trend in the data (P < 0.001) showing a trough towards the end of the light period and a peak in the mid-dark period. There was a similar rhythm for the cells recorded from the peripheral zone (n = 209, P = 0.037). In separate experiments in vitro, to investigate the relationship between mean spike frequency and entropy, potassium-induced depolarization of cells recorded during the subjective night was correlated with a significant increase in mean spike frequency (r = 0.259, P = 0.011) and a decrease in entropy (r = -0.296, P = 0.004). The negative correlation between the entropy and mean spike frequency of cells recorded in vitro was significantly different from that seen in vivo (F = 15.5, P < 0.001), which may reflect differences in the balance between deterministic and stochastic influences on spike occurrence. The study shows that while there is a rhythm of mean spike frequency, parameters based on the variability of interspike interval distributions also display rhythmic changes over the day-night cycle.
Bhumbra
G S
GS
Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK.
Inyushkin
A N
AN
Saeb-Parsy
K
K
Hon
A
A
Dyball
R E J
RE
D000200:Action Potentials / Q000502:physiology* / Q000528:radiation effects; D000465:Algorithms; D000818:Animals; D001683:Biological Clocks / Q000502:physiology* / Q000528:radiation effects; D002940:Circadian Rhythm / Q000502:physiology* / Q000528:radiation effects; D003936:Diagnosis, Computer-Assisted / Q000379:methods; D008027:Light; D008297:Male; D009474:Neurons / Q000502:physiology* / Q000528:radiation effects; D010507:Periodicity; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000502:physiology* / Q000528:radiation effects; D009435:Synaptic Transmission / Q000502:physiology* / Q000528:radiation effects