title: Using light scattering to measure the response of individual ultrasound contrast microbubbles subjected to pulsed ultrasound in vitro.
abstract: Light scattering was used to measure the radial pulsations of individual ultrasound contrast microbubbles subjected to pulsed ultrasound. Highly diluted Optison or Sonazoid microbubbles were injected into either a water bath or an aqueous solution containing small quantities of xanthan gum. Individual microbubbles were insonified by ultrasound pulses from either a commercial diagnostic ultrasound machine or a single element transducer. The instantaneous response curves of the microbubbles were measured. Linear and nonlinear microbubble oscillations were observed. Good agreement was obtained by fitting a bubble dynamics model to the data. The pulse-to-pulse evolution of individual microbubbles was investigated, the results of which suggest that the shell can be semipermeable, and possibly weaken with subsequent pulses. There is a high potential that light scattering can be used to optimize diagnostic ultrasound techniques, understand microbubble evolution, and obtain specific information about shell parameters.
authors.affiliation: Applied Physics Lab, University of Washington, Seattle, Washington 98115, USA.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Guan
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Matula
mesh_terms: D005782:Gels; D066298:In Vitro Techniques; D007834:Lasers; D008027:Light; D016014:Linear Models; D045423:Microbubbles; D008962:Models, Theoretical; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011312:Pressure; D014159:Transducers; D014464:Ultrasonic Therapy / Q000379:methods*; D014463:Ultrasonography / Q000295:instrumentation / Q000379:methods*; D014783:Viscosity
chemical_list: D005782:Gels; D011135:Polysaccharides, Bacterial; C002563:xanthan gum