[The lipid peroxidation mechanism initiated by ischemia-reperfusion injury during renal transplantation].
OBJECTIVE
To explore the mechanism of acute ischemia-reperfusion injury (IRI) to renal-transplant


METHODS
A total of 12 Chinese Sichuan White Pigs (SWPs) (2-3 months old, 17.5 +/- 4.6 kg, with clear family background) were used. 3 pairs of them with better tissue typing were subjected to renal allotransplantation. The other SWPs were allocated to renal autotransplantation. We assessed the SWPs' kidney function, serum superoxide dismutase (SOD) and malonaldehyde (MDA) after transplantation so as to shed light on the possible mechanism of IRI and to find out good detecting methods which could monitor IRI.


RESULTS
Serum creatinine and urea increased in 2 days of postoperation and then returned to normal in both groups except the pigs that suffered from acute tubular necrosis or acute rejection. The concentrations of serum MDA and SOD began to increase 2 days after operation (P < 0.05). More increase of serum MDA and less increase of SOD were detected in renal allotransplant groups than in renal autotransplant groups (P < 0.05). The concentration of serum SOD and MDA were correlated (r = 0.961).


CONCLUSIONS
IRI could initiate lipid peroxidation. IRI could be a single factor or be synergistic with immunologic factors to injure the transplanted organs.
Li
Ping
P
Clinical Laboratory, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Lin
L
Peng
Zhiying
Z
Zhao
Qingjiang
Q
Li
Youping
Y
D000818:Animals; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D011184:Postoperative Period; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000097:blood; D013552:Swine
D008315:Malondialdehyde; D013482:Superoxide Dismutase