Neurokinin-1 receptor interacts with PrP(106-126)-induced dendritic cell migration and maturation.
Circulating monocytic cells may mediate neuroinvasion in transmissible spongiform encephalopathies by transporting prion protein (PrP) from sites of entry to the nervous system. Factors regulating monocyte-derived dendritic cell (DC) functions in neuronal tissue include neurogenic mediators, but their interactions with prion-infected DCs are unknown. Here, we report that neuropeptides regulate the interaction of DCs exposed to PrP(106-126). Inhibition of neurokinin-1-receptors activation with neurokinin-1-receptor antagonist or antibody attenuates substance P-induced enhancement of DC migration induced by PrP(106-126) and prion-protein-induced DC maturation. Other neuropeptides arrest chemotaxis. Data support a priming role of the neuropeptide substance P in PrP(106-126)-induced migration of DCs involving neurokinin-1-receptors.
Kaneider
Nicole C
NC
Division of General Internal Medicine, Department of Internal Medicine, Medical University Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
Kaser
Arthur
A
Dunzendorfer
Stefan
S
Tilg
Herbert
H
Patsch
Josef R
JR
Wiedermann
Christian J
CJ
D000906:Antibodies / Q000494:pharmacology; D018122:B7-1 Antigen / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D003713:Dendritic Cells / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005434:Flow Cytometry / Q000379:methods; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D064729:Neurokinin-1 Receptor Antagonists; D009479:Neuropeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D011328:Prions / Q000494:pharmacology*; D018040:Receptors, Neurokinin-1 / Q000276:immunology / Q000378:metabolism*; D018709:Statistics, Nonparametric
D000906:Antibodies; D018122:B7-1 Antigen; D006684:HLA-DR Antigens; D064729:Neurokinin-1 Receptor Antagonists; D009479:Neuropeptides; D010446:Peptide Fragments; D011328:Prions; D018040:Receptors, Neurokinin-1; C082632:prion protein (106-126)