title: [Screening of cDNA clone for putative RNA polymerase subunit of Cysticercus cellulosae].
abstract: OBJECTIVE
  To obtain related genes of Cysticercus cellulosae from spliced leader (SL) cDNA library.
  
  
  METHODS
  Spliced leader library of Cysticercus cellulosae was constructed using SL specific primer and oligo (dT) 15 with M13M4 primer, and positive clones were then screened randomly, identified with enzyme restriction, followed by sequencing and homologous analysis.
  
  
  RESULTS
  The amino acid sequence, encoded by the positive clone with a poly (A)22 tail and a complete open reading frame (ORF), was with homology of RNA polymerase subunit genes of human, B. napus, fission yeast, A. thaliana, C. elegans and fruit fly up to 71.6%.
  
  
  CONCLUSIONS
  The protein, RNA polymerase subunit encoded putatively by the clone, is high conservative in different species.
authors.affiliation: Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
authors.forename: Xue-nong
authors.initials: XN
authors.lastname: Luo
authors.forename: Ya-dong
authors.initials: YD
authors.lastname: Zheng
authors.forename: Yong-xi
authors.initials: YX
authors.lastname: Dou
authors.forename: Jun-lin
authors.initials: JL
authors.lastname: Hou
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Jing
authors.forename: Xue-peng
authors.initials: XP
authors.lastname: Cai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003551:Cysticercosis / Q000469:parasitology*; D003552:Cysticercus / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020038:RNA, Spliced Leader / Q000235:genetics; D013552:Swine
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D020038:RNA, Spliced Leader; D012321:DNA-Directed RNA Polymerases