Global analysis of protein function using protein microarrays.
Protein microarrays containing thousands of proteins arrayed at high density can be prepared and probed for a wide variety of activities, thereby allowing the large scale analysis of many proteins simultaneously. In addition to identifying the activities of many previously uncharacterized proteins, protein microarrays can reveal new activities of well-characterized proteins, thus providing new insights about the functions of these proteins. Below, we describe the construction and use of protein microarrays and their applications using yeast as a model system.
Smith
Michael G
MG
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Jona
Ghil
G
Ptacek
Jason
J
Devgan
Geeta
G
Zhu
Heng
H
Zhu
Xiaowei
X
Snyder
Michael
M
D000818:Animals; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis*
D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins