Comparison of hemagglutination inhibition test and ELISA in quantification of antibodies to egg drop syndrome virus.
A single-serum dilution ELISA for egg drop syndrome (EDS) virus-specific antibodies was developed. In testing 425 chicken sera it was found to have a 93.6% sensitivity and 98.7% specificity relative to a hemagglutination inhibition (HI) test. The correlation coefficient for ELISA and HI titers was 0.793. The ELISA was efficacious in quantification of both vaccinal and infection antibodies and could routinely be used for screening large numbers of field sera.
Raj
G Dhinakar
GD
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India. dhinakarraj@yahoo.com
Ratnapraba
S
S
Matheswaran
K
K
Nachimuthu
K
K
D000257:Adenoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000259:Aviadenovirus / Q000276:immunology*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D011201:Poultry Diseases / Q000097:blood / Q000517:prevention & control*; D012044:Regression Analysis; D015203:Reproducibility of Results; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines