title: Developmentally regulated alterations in Polycomb repressive complex 1 proteins on the inactive X chromosome.
abstract: Polycomb group (PcG) proteins belonging to the polycomb (Pc) repressive complexes 1 and 2 (PRC1 and PRC2) maintain homeotic gene silencing. In Drosophila, PRC2 methylates histone H3 on lysine 27, and this epigenetic mark facilitates recruitment of PRC1. Mouse PRC2 (mPRC2) has been implicated in X inactivation, as mPRC2 proteins transiently accumulate on the inactive X chromosome (Xi) at the onset of X inactivation to methylate histone H3 lysine 27 (H3-K27). In this study, we demonstrate that mPRC1 proteins localize to the Xi, and that different mPRC1 proteins accumulate on the Xi during initiation and maintenance of X inactivation in embryonic cells. The Xi accumulation of mPRC1 proteins requires Xist RNA and is not solely regulated by the presence of H3-K27 methylation, as not all cells that exhibit this epigenetic mark on the Xi show Xi enrichment of mPRC1 proteins. Our results implicate mPRC1 in X inactivation and suggest that the regulated assembly of PcG protein complexes on the Xi contributes to this multistep process.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA. plath@wi.mit.edu
authors.forename: Kathrin
authors.initials: K
authors.lastname: Plath
authors.forename: Dale
authors.initials: D
authors.lastname: Talbot
authors.forename: Karien M
authors.initials: KM
authors.lastname: Hamer
authors.forename: Arie P
authors.initials: AP
authors.lastname: Otte
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Yang
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jaenisch
authors.forename: Barbara
authors.initials: B
authors.lastname: Panning
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004303:Dosage Compensation, Genetic; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D046911:Macromolecular Substances / Q000378:metabolism; D008745:Methylation; D051379:Mice; D063150:Polycomb Repressive Complex 1; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins / Q000378:metabolism*; D013045:Species Specificity; D014960:X Chromosome / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; D006657:Histones; D046911:Macromolecular Substances; C066770:Pc protein, Drosophila; C103827:Phc1 protein, mouse; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1; D008239:Lysine