title: Genetic variability in ESAG6 genes among Trypanosoma evansi isolates and in comparison to other Trypanozoon members.
abstract: Bloodstream trypanosomes take up iron needed for their propagation through the transferrin receptor that, in Trypanosoma brucei, is encoded by expression-site-associated genes (ESAGs), ESAG6 and 7 genes located in variant surface glycoprotein expression sites. ESAG6 and 7 genes in different expression sites have been shown to encode transferrin receptors with varying affinities for polymorphic transferrins. T. brucei could cope with the different host transferrins by switching between expression sites. ESAG6- and 7-encoded transferrin receptor appear to be present in Trypanosoma evansi but the genes have not yet been characterized. In this study, we cloned and sequenced different members of ESAG6 genes in seven isolates of T. evansi from geographically distinct localities in Thailand. We assessed the intra- and inter-species genetic variability in the transferrin receptor gene regions involved in transferrin binding and established that T. evansi, like T. brucei, has widely diverse ESAG6 genes. In addition, T. evansi possess a clade of ESAG6 variants not observed in T. brucei and different T. evansi strains share at least two conserved variants. We further noted that T. evansi possesses all the reported T. equiperdum ESAG6 variants as a subset. Our findings depict a correlation between the genetic diversity in the transferrin-binding regions of ESAG6 genes with the broad host range of T. evansi and T. brucei compared to the narrow host range of Trypanosoma equiperdum.
authors.affiliation: Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: William H
authors.initials: WH
authors.lastname: Witola
authors.forename: Nopporn
authors.initials: N
authors.lastname: Sarataphan
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
authors.forename: Misao
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000662:veterinary; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D011990:Receptors, Transferrin / Q000235:genetics*; D016415:Sequence Alignment; D013785:Thailand; D014345:Trypanosoma / Q000235:genetics* / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000469:parasitology / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D011990:Receptors, Transferrin