Exhaustive genotyping of the CEM15 (APOBEC3G) gene and absence of association with AIDS progression in a French cohort.
CEM15 (or APOBEC3G) has recently been identified as an inhibitor of human immunodeficiency virus type 1 (HIV-1) replication in vitro. To evaluate the impact of its genetic variations on the progression of acquired immunodeficiency syndrome (AIDS), we have performed an extensive genetic analysis of CEM15. We have sequenced CEM15 in a cohort of 327 HIV-1-seropositive patients with extreme disease progression phenotypes--either slow progression or rapid progression--and in 446 healthy control subjects, all of white descent. We have identified 29 polymorphisms with allele frequencies >1%, 14 of which were newly characterized. There were no significant associations between the polymorphisms or haplotypes of CEM15 and a disease progression phenotype in our cohort.
Do
Hervé
H
Equipe Genomique, Bioinformatique et Pathologies du Systeme Immunitaire, INSERM EMI0355, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
Vasilescu
Alexandre
A
Diop
Gora
G
Hirtzig
Thomas
T
Heath
Simon C
SC
Coulonges
Cédric
C
Rappaport
Jay
J
Therwath
Amu
A
Lathrop
Mark
M
Matsuda
Fumihiko
F
Zagury
Jean-François
JF
D000071480:APOBEC-3G Deaminase; D000163:Acquired Immunodeficiency Syndrome / Q000209:etiology / Q000235:genetics*; D000483:Alleles; D015331:Cohort Studies; D003564:Cytidine Deaminase; D018450:Disease Progression; D005787:Gene Frequency / Q000276:immunology; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006679:HIV Seropositivity; D006239:Haplotypes / Q000276:immunology*; D006801:Humans; D009700:Nucleoside Deaminases; D011506:Proteins / Q000235:genetics*; D012097:Repressor Proteins; D017421:Sequence Analysis
D011506:Proteins; D012097:Repressor Proteins; D009700:Nucleoside Deaminases; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase