Engineering stereocontrol into an aldolase-catalysed reaction.
A novel thermostable aldolase has been developed for synthetic application, and substrate engineering has been used to induce stereocontrol into aldol reactions of this naturally-promiscuous enzyme.
Lamble
Henry J
HJ
Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath, UK BA2 7AY.
Danson
Michael J
MJ
Hough
David W
DW
Bull
Steven D
SD
D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D001698:Biomedical Engineering / Q000379:methods*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004795:Enzyme Stability; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D013237:Stereoisomerism; D016944:Sulfolobus / Q000201:enzymology / Q000235:genetics
D000447:Aldehydes; C116609:3-hydroxybutanal; D005634:Fructose-Bisphosphate Aldolase