Superoxide dismutase failed to attenuate allergen-induced nasal congestion in ragweed-sensitized dogs.
We hypothesized that augmentation of antioxidant defenses with exogenous superoxide dismutase (SOD), an enzyme that provides an initial defense against oxidative injury, would attenuate allergen-induced nasal congestion in the canine model of allergic rhinitis. Nasal congestion was evaluated by the measurements of nasal resistance and the volume of the nasal passage. In five nonsensitized dogs, 30,000 U of SOD from bovine erythrocytes delivered by aerosol to the nasal passages before histamine challenge reduced the histamine-induced nasal congestion. At 30 min postchallenge, nasal resistance was 1.14 +/- 0.2 cmH2O.l(-1).min(-1) in the saline pretreatment study vs. 0.36 +/- 0.02 cmH2O.l(-1).min(-1) in the SOD pretreatment study (P < 0.05), and volume of nasal passage was 10.9 +/- 0.5 cm3 vs. 17.4 +/- 1.3 cm3 (P < 0.05), respectively. In five sensitized dogs, however, neither an analogous pretreatment with SOD nor intranasal aerosolized pretreatment with 30,000 U of SOD conjugated to polyethylene glycol attenuated ragweed-induced nasal congestion. Also, systemic application of SOD did not attenuate responses to challenges with histamine and ragweed in nonsensitized and sensitized dogs, respectively. The antioxidant-induced attenuation of nasal congestion in nonsensitized dogs confirms validity of the model and indicates the involvement of free radical-mediated damage in the genesis of the histamine-induced congestion. In sensitized dogs, the data do not support the hypothesis that oxidative stress is a clinically significant component of acute ragweed-induced nasal congestion. The data do not support the use of SOD for acute protection against allergic rhinitis.
Skorohod
Natalia
N
Dept. of Medicine, M/C 788, Univ. of Illinois at Chicago, Chicago, IL 60612, USA.
Yeates
Donovan B
DB
D000280:Administration, Inhalation; D000485:Allergens / Q000009:adverse effects*; D031164:Ambrosia / Q000009:adverse effects*; D000818:Animals; D004285:Dogs; D006632:Histamine; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D015508:Nasal Obstruction / Q000175:diagnosis / Q000188:drug therapy* / Q000209:etiology*; D009299:Nasal Provocation Tests / Q000379:methods; D011058:Pollen / Q000009:adverse effects*; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000188:drug therapy* / Q000209:etiology*; D012720:Severity of Illness Index; D013482:Superoxide Dismutase / Q000008:administration & dosage*; D017211:Treatment Failure
D000485:Allergens; D006632:Histamine; D013482:Superoxide Dismutase