title: Thioredoxin peroxidase secreted by Fasciola hepatica induces the alternative activation of macrophages.
abstract: Alternatively activated macrophages (AAMphi) are primarily associated with the chronic stages of parasitic infections and the development of a polarized Th2 response. We have shown that Fasciola hepatica infection of BALB/c mice induces a polarized Th2 response during both the latent and chronic stage of disease. The activation status of macrophages was analyzed in this model of helminth infection by evaluating the expression of genetic markers of alternative activation, namely, Fizz1, Ym1, and Arg1. AAMphi were recruited to the peritoneum of mice within 24 h of F. hepatica infection and after intraperitoneal injection of parasite excretory-secretory (ES) products. Administration of a recombinant antioxidant thioredoxin peroxidase (TPx), which is contained within the ES products, also induced the recruitment of AAMphi to the peritoneum. In vitro studies showed that this recombinant TPx directly converts RAW 264.7 macrophages to an alternatively activated phenotype characterized by the production of high levels of interleukin-10 (IL-10), prostaglandin E(2), corresponding with low levels of IL-12. Our data suggest that the Th2 responses induced by the helminth F. hepatica are mediated through the secretion of molecules, one of which is TPx, that induce the recruitment and alternative activation of macrophages.
authors.affiliation: Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Westbourne St., Gore Hill, Sydney, NSW 2065, Australia. Sheila.Donnelly@uts.edu.au
authors.forename: Sheila
authors.initials: S
authors.lastname: Donnelly
authors.forename: Sandra M
authors.initials: SM
authors.lastname: O'Neill
authors.forename: Mary
authors.initials: M
authors.lastname: Sekiya
authors.forename: Grace
authors.initials: G
authors.lastname: Mulcahy
authors.forename: John P
authors.initials: JP
authors.lastname: Dalton
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005210:Fasciola hepatica / Q000201:enzymology*; D005211:Fascioliasis / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000502:physiology; D008262:Macrophage Activation; D008264:Macrophages / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010544:Peroxidases / Q000502:physiology*; D054464:Peroxiredoxins; D010641:Phenotype; D018418:Th2 Cells / Q000276:immunology
chemical_list: D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma; D010544:Peroxidases; D054464:Peroxiredoxins