[Determination of lignans of Schisandra medicinal plants by HPLC].
OBJECTIVE
To determinate the contents of lignans in the stems, roots and fruits of Schisandra medicinal plants.


METHODS
HPLC was applied to determine the contents of schisandrin, gomisin A, schisantherin A, deoxyschizandrin, d-epigalbacine, (+)-anwulignan, wuweizisu B, 6-O-benzoylgomisin O and wuweizisu C in the stems, roots and fruits of Schisandra.


RESULTS
The percent contents of 9 lignans in the fruits of 5 species were 0.52% - 1.96%, and 0.02% - 1.51% in the stems of 11 species. The lignans in the fruits of S. micrantha were similar to that of S. sphenanthera, especially with high content of deoxyschizandrin. The lignans of S. viridis were similar to that of S. chinensis and with high content of schisandrin, gomisin A and wuweizisu B. (+)-Anwulignan was present in the fruits of S. henryi and S. sphenanthera, in the stems of S. micrantha and S. pubescens var. pubinervis, and in the roots of S. bicolor with percentages of 0.77%, 0.42%, 0.50%, 0.26% and 0.38% respectively. d-Epigalbacine was present in the stems of S. pubescens var. pubinervis, S. pubescens and S. glaucescens with percentages of 0.89%, 1.51% and 0.17% respectively.


CONCLUSIONS
The lignans contents in the fruits of Schisandra were higher than that in the roots and stems. The fruits of S. henryi and S. sphenanthera, the stems of S. micrantha and S. pubescens var. pubinervis and the roots of S. bicolor, the stems of S. pubescens var. pubinervis, S. pubescens and S. glaucescens may be served as the resources plants of (+)-anwulignan and d-epigalbacine respectively.
Wang
Yan-han
YH
Deparment of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 200032, China.
Gao
Jian-ping
JP
Chen
Dao-feng
DF
D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry; D017705:Lignans / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D032141:Schisandra / Q000737:chemistry* / Q000145:classification; D013045:Species Specificity
D017705:Lignans