title: Genome evolution in the genus Sorghum (Poaceae).
abstract: OBJECTIVE
  The roles of variation in DNA content in plant evolution and adaptation remain a major biological enigma. Chromosome number and 2C DNA content were determined for 21 of the 25 species of the genus Sorghum and analysed from a phylogenetic perspective.
  
  
  METHODS
  DNA content was determined by flow cytometry. A Sorghum phylogeny was constructed based on combined nuclear ITS and chloroplast ndhF DNA sequences.
  
  
  RESULTS
  Chromosome counts (2n = 10, 20, 30, 40) were, with few exceptions, concordant with published numbers. New chromosome numbers were obtained for S. amplum (2n = 30) and S. leiocladum (2n = 10). 2C DNA content varies 8.1-fold (1.27-10.30 pg) among the 21 Sorghum species. 2C DNA content varies 3.6-fold from 1.27 pg to 4.60 pg among the 2n = 10 species and 5.8-fold (1.52-8.79 pg) among the 2n = 20 species. The x = 5 genome size varies over an 8.8-fold range from 0.26 pg to 2.30 pg. The mean 2C DNA content of perennial species (6.20 pg) is significantly greater than the mean (2.92 pg) of the annuals. Among the 21 species studied, the mean x = 5 genome size of annuals (1.15 pg) and of perennials (1.29 pg) is not significantly different. Statistical analysis of Australian species showed: (a) mean 2C DNA content of annual (2.89 pg) and perennial (7.73 pg) species is significantly different; (b) mean x = 5 genome size of perennials (1.66 pg) is significantly greater than that of the annuals (1.09 pg); (c) the mean maximum latitude at which perennial species grow (-25.4 degrees) is significantly greater than the mean maximum latitude (-17.6) at which annual species grow.
  
  
  CONCLUSIONS
  The DNA sequence phylogeny splits Sorghum into two lineages, one comprising the 2n = 10 species with large genomes and their polyploid relatives, and the other with the 2n = 20, 40 species with relatively small genomes. An apparent phylogenetic reduction in genome size has occurred in the 2n = 10 lineage. Genome size evolution in the genus Sorghum apparently did not involve a 'one way ticket to genomic obesity' as has been proposed for the grasses.
authors.affiliation: Department of Soil and Crop Sciences, Texas Agricultural Experiment Station, Texas A&M University, College Station, TX 77843-2474, USA. hj-price@tamu.edu
authors.forename: H James
authors.initials: HJ
authors.lastname: Price
authors.forename: Sally L
authors.initials: SL
authors.lastname: Dillon
authors.forename: George
authors.initials: G
authors.lastname: Hodnett
authors.forename: William L
authors.initials: WL
authors.lastname: Rooney
authors.forename: Larry
authors.initials: L
authors.lastname: Ross
authors.forename: J Spencer
authors.initials: JS
authors.lastname: Johnston
mesh_terms: D002467:Cell Nucleus / Q000235:genetics; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D019143:Evolution, Molecular; D018745:Genome, Plant; D007621:Karyotyping; D010802:Phylogeny; D045868:Sorghum / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant