Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata.
Nitrate at 70 mM gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3- molar ratio of 5:1 (giving a total of 40 mM N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l(-1), 0.36 mg g(-1), and 12.8 mg l(-1), respectively.
Pan
Xue-Wu
XW
Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, P.R. China.
Xu
Heng-Hao
HH
Liu
Xin
X
Gao
Xiang
X
Lu
Ying-Tang
YT
D029769:Camptotheca / Q000254:growth & development* / Q000378:metabolism*; D002166:Camptothecin / Q000096:biosynthesis* / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation
D002166:Camptothecin