Identification of more than 200 glucose-responsive Arabidopsis genes none of which responds to 3-O-methylglucose or 6-deoxyglucose.
The response of some plant genes to glucose analogues 3-O-methylglucose (3OMG) or 6-deoxyglucose (6DOG) has been cited as evidence for metabolism-independent glucose signalling. To analyse such signalling using a genetic approach, we sought to identify Arabidopsis glucose-responsive genes which also respond to 3OMG and 6DOG in seedlings. Microarray analysis of gene expression in glucose-treated seedlings and RT-PCR analysis of glucose-treated leaf sections identified more than 200 glucose-responsive genes, but none responded to 3OMG or 6DOG. These data together with other published data on individual genes fail to identify any Arabidopsis sugar-responsive genes which also respond to 3OMG or 6DOG.
Villadsen
Dorthe
D
Institute of Cell and Molecular Biology, University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh, EH9 3JH, UK.
Smith
Steven M
SM
D019325:3-O-Methylglucose / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018749:RNA, Plant; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D005947:Glucose; C037904:6-deoxyglucose