[The effect of jiunaoyizhi capsul on signal transduction pathway of SY5Y cell lines].
OBJECTIVE
To observe the effect of Jiunaoyizhi capsul on the signal transduction pathway of SY5Y cell lines and explore the mechanism of the function of the capsul's enhancing neuronal growth.


METHODS
Human neuroblastoma was used as cell models and they were divided into control group and experimental group. Supernatant of cell lysate was taken and immunoprecipitation was done with antibodies to proteins related to signal transduction pathway, and the immunoprecipitates were analyzed by Western blotting.


RESULTS
After treatment with Jiunaoyizhi capsul, expression of Akt/PKB, CREB, P-CREB was clearly increased and expression of cytochrome C decreased more than the control group.


CONCLUSIONS
Jiunaoyizhi capsul can promote expression of some proteins related with signal transduction pathway in SY5Y cell lines. Mechanism of Jiunaoyizhi capsul's enhancing neuronal growth is relevant to expression of some proteins in signal transduction pathway.
Meng
Yan
Y
Beijing Aging Research Center, XuanWu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
Zhang
Jing-yan
JY
Wang
Rong
R
Ji
Zhi-juan
ZJ
Sheng
Shu-li
SL
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D015398:Signal Transduction
D017362:Cyclic AMP Response Element-Binding Protein; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D045304:Cytochromes c