Expression of maf-B mRNA in the epithelium around the eyelid closure of the mouse eye at embryonic day 18.
Maf encodes a transcription factor protein containing a typical basic leucine zipper domain structure, a motif for protein dimerization and DNA binding. We examined the expression of maf-B mRNA in the epithelium around the eyelid closure. Expression of maf-B mRNA was examined in C57Bl6 mice at the embryonic stages in 12.5 days of gestation (E12.5) and E18 using in situ hybridization with 35S-labeled antisense riboprobes. In embryos studied 12.5 days postconception, a message specific for maf-B was not detected around the developing eyelid. In contrast, maf-B was strongly expressed in the epithelium of the eyelid closure at E18. Expression of maf-B was strongly noted in the suprabasal differentiating cells derived from the basal layer of the conjunctiva and epidermis. In contrast, basal cells in the eyelid closure and in the epidermis, as well as keratinizing cells, did not express maf-B. These data indicate that maf-B mRNA is expressed during development of the eyelid closure.
Kase
Satoru
S
Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, 060-8638, Sapporo, Japan.
Imaki
Junko
J
Harada
Takayuki
T
Harada
Chikako
C
Ohgami
Kazuhiro
K
Shiratori
Kenji
K
Sakai
Masaharu
M
Nishi
Shinzo
S
Ohno
Shigeaki
S
Yoshida
Kazuhiko
K
D000818:Animals; D030161:Avian Proteins / Q000235:genetics; D003228:Conjunctiva / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005143:Eyelids / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D007633:Keratins / Q000378:metabolism; D051258:MafB Transcription Factor; D051379:Mice; D008810:Mice, Inbred C57BL; D015513:Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*
D030161:Avian Proteins; D004268:DNA-Binding Proteins; D051258:MafB Transcription Factor; C490406:Mafb protein, mouse; D015513:Oncogene Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D007633:Keratins