Detection of single base mismatches and abasic sites using phenanthridinium as an artificial DNA base and charge donor.
Combining the fluorescence properties of phenanthridinium as an artificial DNA base together with DNA-mediated charge transfer processes allows the homogeneous detection of DNA base mismatches and abasic sites.
Valis
Linda
L
Technical University Munich, Chemistry Department, Lichtenbergstr. 4, D-85747, Garching, Germany.
Amann
Nicole
N
Wagenknecht
Hans-Achim
HA
D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D004579:Electron Transport; D004996:Ethidium / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D015394:Molecular Structure; D010617:Phenanthridines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D010617:Phenanthridines; C014740:phenanthridinium; D006147:Guanine; D004247:DNA; D004996:Ethidium; C066856:7-deazaguanine