Scanning electrochemical microscopy for detection of biosensor and biochip surfaces with immobilized pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase as enzyme label.
Scanning electrochemical microscopy (SECM) was applied to study quinoprotein-based biosensor or biochip. A typical quinoprotein, pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH), was taken as example. Feedback mode and generation collection (GC) mode in SECM have been explored in imaging the catalytic activity of GDH on microscopic magnetic bead domains. Biotinylated GDH was immobilized by using streptavidin-coated paramagnetic microbeads, which were deposited as microspot on a hydrophobic surface. Ferrocenemethanol and ferricyanide were used as electron mediators for feedback and GC detection, respectively. Enzymatic catalysis was further studied quantitatively using the theory developed for SECM.
Zhao
Chuan
C
Institute of Pure and Applied Chemistry and Institute of Chemistry and Biology of the Marine Environment (ICBM), School of Mathematics and Natural Sciences, Carl von Ossietzky University of Oldenburg, D-26111 Oldenburg, Germany. chuan.zhao@uni-oldenburg.de
Wittstock
Gunther
G
D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D042843:Glucose 1-Dehydrogenase; D045542:PQQ Cofactor
D045542:PQQ Cofactor; D042843:Glucose 1-Dehydrogenase