Pi30 DNA probe may be useful for the identification of Prevotella intermedia at the species or strain level.
Recently, we introduced a new method for the rapid screening of bacterial species-or subspecies-specific DNA probes, named the "inverted dot blot hybridization screening method." This method has subsequently been then applied to develop species-or strain-specific DNA probes for Prevotella intermedia and Prevotella nigrescens. In a previous study, the inverted dot blot hybridization data showed that a probe, Pi30, was specific for P. intermedia. In this study, the DNA probe Pi30 was evaluated by Southern blot analysis to determine if it could distinguish P. intermedia from P. nigrescens. The data showed that the probe Pi30 reacted with the genomic DNAs from the reference strains and clinical isolates of both P. intermedia and P. nigrescens, but the size of the signal bands was different. In addition, the probe Pi30 reacted with a 1.4 kbp fragment from the genomic DNAs digested with Pst I of the P. intermedia strains but not with any fragments of P. nigrescens strains. The result indicates that the probe Pi30 could be useful for the identification of P. intermedia by restriction fragment length polymorphism (RFLP) at the species or strain level.
Shin
Yong Kook
YK
Division of Genome Resources Bank and Reservation, National Genome Research Institute, National Institute of Health, 5 Nokbun-Dong, Eunpyn-Gu, Seoul 122-701, Republic of Korea.
Jeong
Seung-U
SU
Yoo
So Young
SY
Kim
Mi-Kwang
MK
Kim
Hwa-Sook
HS
Kim
Byung-Ock
BO
Kim
Do Kyung
DK
Hwang
Ho-Keel
HK
Kook
Joong-Ki
JK
D016866:Bacteroidaceae Infections / Q000382:microbiology*; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010518:Periodontitis / Q000382:microbiology*; D018713:Prevotella intermedia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D045242:Prevotella nigrescens / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
D015342:DNA Probes; D004269:DNA, Bacterial