title: Polysorbate 80 increases the susceptibility to oxidative stress in rat thymocytes.
abstract: Effect of simultaneous application of polysorbate 80, a nonionic surfactant widely used in pharmaceutical products, and hydrogen peroxide on rat thymocytes was examined to see if polysorbate 80 increases the susceptibility to oxidative stress because this surfactant decreases the cellular content of glutathione. Polysorbate 80 at clinically-relevant concentrations increases the cytotoxicity of hydrogen peroxide under the in vitro condition. Result suggests that polysorbate 80 may increase the susceptibility of cells to oxidative stress.
authors.affiliation: Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tatsuishi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Oyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Iwase
authors.forename: Jun-ya
authors.initials: JY
authors.lastname: Yamaguchi
authors.forename: Masako
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Aimi
authors.initials: A
authors.lastname: Kanada
authors.forename: Seigo
authors.initials: S
authors.lastname: Hirama
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005079:Excipients / Q000633:toxicity*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D011136:Polysorbates / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology*; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D017304:Annexin A5; D005079:Excipients; D005456:Fluorescent Dyes; D011136:Polysorbates; D014966:Xanthenes; C059715:Fluo-3; D006861:Hydrogen Peroxide