title: [Effects of XW630 on the expression of type I collagen protein in the epiphyseal plates of mice].
abstract: OBJECTIVE
  To identify the effect of XW630 on the expression of type I collagen in the epiphyseal plates of mice.
  
  
  METHODS
  The immunohistochemical method was used to measure the change of type I collagen protein after being treated with XW630.
  
  
  RESULTS
  With 10(-7) mol/L or 10(-7) mol/L of XW630, the area of positive stain cells in every zone increased significantly compared to the control group. A more significant increase of the area of positive cells in the proliferative zone and hypertrophic zone was observed in the XW630 group than in the estrone group at a concentration of 10(-7) mol/L. When the XW630 was decreased to 10(-9) mol/L, the increase of positive cells was only found in the hypertrophic zone. The area of positive cells increased with the concentration of XW630.
  
  
  CONCLUSIONS
  The XW630 upregulates the dose-dependent expression of type I collagen protein in the resting zone, proliferative zone, and hypertrophic zone in the epiphyseal plates of mice in vitro.
authors.affiliation: Department of Medicinal Chemistry, West China Pharmaceutical College, Sichuan University, Chengdu 610041, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Li
authors.forename: Lingling
authors.initials: L
authors.lastname: Weng
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rongliang
authors.initials: R
authors.lastname: Lou
authors.forename: Hu
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D006132:Growth Plate / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D009924:Organ Culture Techniques; D010879:Piperazines / Q000494:pharmacology*; D013754:Tetracyclines / Q000494:pharmacology*; D014457:Ulna / Q000378:metabolism
chemical_list: C428143:2-(3-estrone-N-ethyl-piperazine-methyl)tetracycline; D024042:Collagen Type I; D010879:Piperazines; D013754:Tetracyclines