[A new in vitro model to quantify angiogenesis].
At present the therapeutical use of proangiogenic factors is in the focus of interest. Quantitation of angiogenesis, which is done particularly in animal models, is a prerequisite for the determination of the angiogenic effect of a particular substance. In the frame of this study a new method for quantitation of angiogenesis in vitro was established. Endothelial cells were incubated with angiogenic factors to be tested, and documented photographically in defined microscopical sectors over a certain time period. Subsequently defined stages of angiogenesis (1-8) were assigned. For each sector all stages assigned were summarized and compared with each other. This and additional morphometric studies showed that angiogenesis up to the development of lumenised capillary-like structures in vitro can be quantified. Validation of the method showed that routine and reproducible accomplishment should be possible for different investigators with a maintainable effort of time and costs.
Bahramsoltani
Mahtab
M
Fachbereich Veterinaermedizin, Institut für Veterinaer-Anatomie, Freie Universität Berlin, Koserstrasse 20, D-14195 Berlin, Germany. bahramsoltani.mahtab@vetmed.fu-berlin.de
Plendl
Johanna
J
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000826:Animal Testing Alternatives; D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology*
D043925:Angiogenesis Inducing Agents