title: Characterization of an ExoS Type III translocation-resistant cell line.
abstract: Pseudomonas aeruginosa ExoS is a type III-secreted type III-secreted, bifunctional protein that causes diverse effects on eukaryotic cell function. The coculture of P. aeruginosa strains expressing ExoS with HL-60 myeloid cells revealed the cell line to be resistant to the toxic effects of ExoS. Differentiation of HL-60 cells with phorbol 12-myristate 13-acetate (TPA) rendered the cell line sensitive to ExoS. To understand the cellular basis for the alteration in sensitivity, undifferentiated and TPA-differentiated HL-60 cells were compared for differences in bacterial adherence, type III secretion induction, and ExoS translocation. These comparisons found that ExoS was translocated more efficiently in TPA-differentiated HL-60 cells than in undifferentiated cells. The studies support the ability of eukaryotic cells to influence P. aeruginosa TTS at the level of membrane translocation.
authors.affiliation: Department of Microbiology, Immunology, and Cell Biology, Robert C. Byrd Health Sciences Center, P.O. Box 9177, West Virginia University, Morgantown, WV 26506-9177, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Rucks
authors.forename: Joan C
authors.initials: JC
authors.lastname: Olson
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D001427:Bacterial Toxins / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D021381:Protein Transport; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D001427:Bacterial Toxins; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; C444820:exoenzyme S; D013755:Tetradecanoylphorbol Acetate