Analysis of the major patterns of B cell gene expression changes in response to short-term stimulation with 33 single ligands.
We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca(2+) and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L.
Zhu
Xiaocui
X
Molecular Biology Laboratory, Alliance for Cellular Signaling, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Hart
Rebecca
R
Chang
Mi Sook
MS
Kim
Jong-Woo
JW
Lee
Sun Young
SY
Cao
Yun Anna
YA
Mock
Dennis
D
Ke
Eugene
E
Saunders
Brian
B
Alexander
Angela
A
Grossoehme
Joella
J
Lin
Keng-Mean
KM
Yan
Zhen
Z
Hsueh
Robert
R
Lee
Jamie
J
Scheuermann
Richard H
RH
Fruman
David A
DA
Seaman
William
W
Subramaniam
Shankar
S
Sternweis
Paul
P
Simon
Melvin I
MI
Choi
Sangdun
S
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism / Q000494:pharmacology; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D023201:CD40 Ligand / Q000378:metabolism / Q000494:pharmacology; D049109:Cell Proliferation; D002478:Cells, Cultured; D018899:CpG Islands / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000276:immunology*; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology; D008024:Ligands; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000235:genetics* / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D008024:Ligands; D008070:Lipopolysaccharides; D023201:CD40 Ligand; D015847:Interleukin-4