Rubella virus infection dysregulates the pattern of p63 expression.
The effect of rubella virus (RV) on the expression of the p63 isoforms was investigated in Vero cells. The levels of all the TAp63 isoforms were elevated, while the expression of a approximately 73 kDa isoform corresponding to DeltaNp63alpha was downregulated in Vero cells infected with the To-336 strain of RV. A approximately 66 kDa isoform corresponding to TAp63beta was the predominant protein species in RV-infected cells. Semi-quantitative end-point dilution RT-PCR analysis, with TAp63beta isoform-specific primers, detected a 4-fold rise in the TAp63beta mRNA level following virus infection. Taken together, our data demonstrate that RV infection alters the stoichiometric ratio of the p63 isoforms. The dysregulated pattern of p63 expression observed in RV-infected cells may represent a mechanism whereby RV exerts its pro-apoptotic effect.
Buzás
Krisztina
K
Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.
Miczák
András
A
Degré
Miklos
M
Megyeri
Klára
K
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012409:Rubella / Q000235:genetics / Q000473:pathology*; D012412:Rubella virus / Q000502:physiology*; D014709:Vero Cells
D020033:Protein Isoforms