title: The attraction of Mac-1+ phagocytes during acute inflammation by methyl-coated self-assembled monolayers.
abstract: We have used self-assembled monolayers (SAMs) of alkanethiolates on gold to study the contribution of methyl terminal functional groups in implant-triggered inflammation. The CH3-coated biomaterials were inserted in an air-pouch cavity of the BALB/c mouse and the in situ inflammatory response was monitored 4, 24, 48 and 72 h later. Flow cytometry was applied to define surface expression of the adhesion receptor Mac-1 (CD11b/CD18), a marker of activated leukocytes, and also of CD3 and B220 antigens (T and B lymphocytes). The CH3-coated surfaces caused a significant enhancement in the number of Mac-1+ cells in the implant. The only significant change in T and B lymphocytes was a transient increase in T cells detected 48 h after the implantation. Peak numbers of Mac-1+ phagocytes were observed 24 h after implantation. We conclude that if CH3 is present at the surface of implants, this chemical group will trigger a significant enhancement of activated phagocytes involved in the inflammatory reaction, and this phenomenon may extend the local phlogistic event.
authors.affiliation: INEB-Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. judite@ibmc.up.pt
authors.forename: Judite N
authors.initials: JN
authors.lastname: Barbosa
authors.forename: Pedro
authors.initials: P
authors.lastname: Madureira
authors.forename: Mário A
authors.initials: MA
authors.lastname: Barbosa
authors.forename: Artur P
authors.initials: AP
authors.lastname: Aguas
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D039481:CD11b Antigen / Q000378:metabolism*; D018821:CD18 Antigens / Q000378:metabolism*; D017252:CD3 Complex / Q000096:biosynthesis; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods; D006046:Gold / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism*; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018375:Neutrophil Activation; D010586:Phagocytes / Q000166:cytology*; D010587:Phagocytosis; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D039481:CD11b Antigen; D018821:CD18 Antigens; D017252:CD3 Complex; D020099:Coated Materials, Biocompatible; D013438:Sulfhydryl Compounds; D006046:Gold