title: Autofluorescence lifetime imaging of cultivated cells using a UV picosecond laser diode.
abstract: Lifetime images of autofluorescence of cultivated endothelial cells were recorded using a novel picosecond laser diode in the near ultraviolet range (375 nm). In contrast to existing picosecond light sources this wavelength permits efficient excitation of the free and protein bound coenzyme NADH with fluorescence lifetimes of 0.4-0.5 ns and 2.0-2.5 ns, respectively. The effective fluorescence lifetime tau(eff) (depending on both lifetimes) was homogenously distributed over the cells with some shortening in the perinuclear region, possibly close to mitochondria. A slight decrease of tau(eff) was observed after inhibition of the mitochondrial respiratory chain, whereas a slight increase was observed after inhibition of the glycolytic pathway, thus indicating variations of the ratio of free and protein bound NADH. Although present applications are still limited by their low pulse energy (< or = 5 pJ), uv picosecond laser diodes have a large potential in high resolution fluorescence microscopy and fluorescence lifetime endoscopy.
authors.affiliation: Fachhochschule Aalen, Institut für Angewandte Forschung, Beethovenstr. 1,73430 Aalen, Germany. herbert.schneckenburger@fh-.aalen.de
authors.forename: Herbert
authors.initials: H
authors.lastname: Schneckenburger
authors.forename: Michael
authors.initials: M
authors.lastname: Wagner
authors.forename: Petra
authors.initials: P
authors.lastname: Weber
authors.forename: Wolfgang S L
authors.initials: WS
authors.lastname: Strauss
authors.forename: Reinhard
authors.initials: R
authors.lastname: Sailer
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002460:Cell Line; D003847:Deoxyglucose / Q000494:pharmacology; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D009249:NADP / Q000737:chemistry / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D012402:Rotenone; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D003847:Deoxyglucose