title: Improved long-term cryostorage of Escherichia coli competent cells using trehalose.
abstract: Trehalose added to storage buffers for chemical-induced transformations at 200 mM increased the survival of Escherichia coli competent cells 1.3 approximately 4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.
authors.affiliation: Department of Biochemistry, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. thahn@chonnam.ac.kr
authors.forename: Taeho
authors.initials: T
authors.lastname: Ahn
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Kang
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Yun
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D014199:Trehalose / Q000494:pharmacology*
chemical_list: D003451:Cryoprotective Agents; D014199:Trehalose