Leucobacter chromiireducens sp. nov, and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment.
Two strains designated strains L-1T and L-9T were isolated from activated sludge of a treatment plant that receives wastewater from the tannery industry contaminated with chromium. Phylogenetic analysis showed that the organisms represented two new species of the genus Leucobacter. Strains L-1T and L-9T could be distinguished from the type strain of L. komagatae and from the type strain of "L. albus" by the B-type peptidoglycan composition, fatty acid composition, several phenotypic and physiological characteristics. The major fatty acids of the organisms were iso- and anteiso-branched C15:0 and C17:0, straight-chain C16:0 was also found in relatively high proportions. The organisms were halotolerant, grew in medium containing 9% NaCl, and all strains, including the type strain of L. komagatae grew in medium containing 5 mM Cr(VI). On the basis of the distinct peptidoglycan composition, 16S ribosomal DNA sequence analysis, percentage of DNA-DNA reassociation values, and phenotypic characteristics we are of the opinion that strain L-1T represents a new species of the genus Leucobacter for which we propose the name Leucobacter chromiireducens and that strain L-9T represents an additional new species of the same genus for which we propose the name Leucobacter aridicollis.
Morais
Paula V
PV
Departamento Bioquimica, Faculdade de Ciências e Tecnologia da Universidade de Coimbra, Coimbra, Portugal. pvmorais@ci.uc.pt
Francisco
Romeu
R
Branco
Rita
R
Chung
Ana Paula
AP
da Costa
Milton S
MS
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D002857:Chromium / Q000378:metabolism / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004785:Environmental Pollutants; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009997:Osmotic Pressure; D010457:Peptidoglycan / Q000737:chemistry / Q000302:isolation & purification; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D005227:Fatty Acids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D012722:Sewage; D002857:Chromium; C074702:chromium hexavalent ion