[Expression of telomere repeat binding factor 1 (TRF1) protein in kidney cancer].
To investigate the expression levels of telomere repeat binding factor 1(TRF1) protein in normal kidney tissue and kidney cancer.



Specimens of kidney cancer and pericancerous tissues were collected from 32 cases of renal carcinoma. A quantitative Western blotting technique was developed using TRF1 monoclonal antibody to determine the expression level of TRF1 protein in total protein extracts from tissue specimens.



The expression level of TRF1 protein was higher in normal kidney tissues (3.611 +/-1.922 microg/microl) than that of cancer tissues (2.428 +/-1.352 microg/microl) (t=5.776, P<0.01).



The expression level of TRF1 protein is significantly reduced in kidney cancer and the level is negatively correlated with malignant degree of the cancer.
Shi
Ji-min
JM
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Ding
Wei
W
Huang
He
H
Zhang
Zhi-gen
ZG
Tang
Li-dan
LD
Lin
Mao-fang
MF
D018262:Adenocarcinoma, Clear Cell / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D035321:Telomeric Repeat Binding Protein 1 / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D035321:Telomeric Repeat Binding Protein 1