Crystallization and preliminary X-ray diffraction analysis of suramin, a highly charged polysulfonated napthylurea, complexed with a myotoxic PLA2 from Bothrops asper venom.
Suramin is a highly charged polysulfonated napthylurea that interferes in a number of physiologically relevant processes such as myotoxicity, blood coagulation and several kinds of cancers. This synthetic compound was complexed with a myotoxic Lys49 PLA(2) from Bothrops asper venom and crystallized by the hanging-drop vapor diffusion method at 18 degrees C. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit cell parameters a=49.05, b=63.84 and c=85.67 angstroms. Diffraction data was collected to 1.78 angstroms.
Murakami
Mário T
MT
Departamento de Física, IBILCE, Universidade Estadual Paulista, Cristóvão Colombo, 2265, Nazareth, 15054-000, São José do Rio Preto, SP, Brazil.
Gava
Lisandra M
LM
Zela
Sandro P
SP
Arruda
Emerson Z
EZ
Melo
Paulo A
PA
Gutierrez
José M
JM
Arni
Raghuvir K
RK
D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000737:chemistry* / Q000201:enzymology; D018360:Crystallography, X-Ray; D054501:Group II Phospholipases A2; D008239:Lysine / Q000737:chemistry; D015394:Molecular Structure; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000633:toxicity*; D054467:Phospholipases A2; D013498:Suramin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D014508:Urea / Q000031:analogs & derivatives* / Q000378:metabolism*; D014961:X-Ray Diffraction
D003435:Crotalid Venoms; D013498:Suramin; D014508:Urea; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; D008239:Lysine