Odor-concentration coding in the guinea-pig piriform cortex.
By optical imaging of intrinsic signals, we demonstrated a possible code for odor concentration in the anterior piriform cortex of the guinea-pig. Odor-induced cortical activation, which primarily originated in layer II, appeared in a narrow band beneath the rhinal sulcus over the lateral olfactory tract, corresponding to the dorsal part of the anterior piriform cortex. Lower concentrations activated the rostral region of the band, whereas higher ones generated caudally spreading activation, and the site at which neural activation reached its maximum extent depended upon odor concentration. Different odors with low concentrations generated distinct but somewhat overlapping patterns in the rostral region of the band; the limited extent of cortical activity may be one focal domain for each odor. It was hard to judge, however, that odor-specific domains appeared in the anterior piriform cortex, because the strong stimuli induced largely overlapping patterns. Furthermore, the total area activated increased in proportion to concentrations raised to a power of 0.5-0.9. Importantly, these imaging results were confirmed with unit recordings which indicated a rostro-caudal gradient in odor-sensitivity among cortical neurons. Our results suggest that the dorsal part of the anterior piriform cortex may be associated with odor concentration. Therefore, in addition to recruitment of more olfactory sensory cells and glomeruli in response to stronger stimuli, a rostro-caudal gradient in axonal projections from mitral/tufted cells and/or in association fibers may play an important role in odor-concentration coding in the anterior piriform cortex.
Sugai
T
T
Department of Physiology, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan.
Miyazawa
T
T
Fukuda
M
M
Yoshimura
H
H
Onoda
N
N
D000818:Animals; D001931:Brain Mapping; D000440:Butanols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D007091:Image Processing, Computer-Assisted; D009474:Neurons; D009812:Odorants; D009833:Olfactory Pathways / Q000166:cytology / Q000502:physiology*; D000439:Pentanols / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D012903:Smell / Q000502:physiology*; D014992:Xylenes / Q000494:pharmacology
D000440:Butanols; D000439:Pentanols; D014992:Xylenes; C005716:amyl acetate