title: Reproductive responses of common carp (Cyprinus carpio) exposed in cages to influent of the Las Vegas Wash in Lake Mead, Nevada, from late winter to early spring.
abstract: The Las Vegas Wash (LW) delivers tertiary-treated municipal wastewater effluent, nonpotable shallow groundwater seepage, and runoff from the urbanized Las Vegas Valley to Las Vegas Bay (LX) of Lake Mead. To investigate the potential for contaminants in LW influent to produce effects indicative of endocrine disruption in vivo, adult male and female common carp (Cyprinus carpio) were exposed in cages for 42-48 d at four sites in Lake Mead: LW, LX, and two reference locations in the lake. End points examined included gonadosomatic index; gonad histology; concentrations of plasma vitellogenin (VTG) and plasma sex steroids (17beta-estradiol (E2), testosterone (T), 11-ketotestosterone (11-KT)); plasma estrogen:androgen ratios (E2:T, E2:11-KT), in vitro production of T by gonad tissue, and hepatopancreas ethoxyresorufin O-deethylase activity. Few differences among fish caged at different sites were potentially attributable to exposure to contaminants
authors.affiliation: Department of Zoology, National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA. SnyderEM@bv.com
authors.forename: Erin M
authors.initials: EM
authors.lastname: Snyder
authors.forename: Shane A
authors.initials: SA
authors.lastname: Snyder
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Kelly
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Gross
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Villeneuve
authors.forename: Scott D
authors.initials: SD
authors.lastname: Fitzgerald
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Villalobos
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
mesh_terms: D000728:Androgens / Q000097:blood; D000818:Animals; D002347:Carps / Q000502:physiology*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004967:Estrogens / Q000097:blood; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood; D006066:Gonads / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D008297:Male; D009505:Nevada; D012098:Reproduction / Q000187:drug effects / Q000502:physiology*; D012621:Seasons; D012722:Sewage / Q000737:chemistry*; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000728:Androgens; D004967:Estrogens; D012739:Gonadal Steroid Hormones; D012722:Sewage; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1