Detection of tumour cells in the peripheral blood of patients with breast cancer. Development of a new sensitive and specific immunomolecular assay.
Malignant cells in the peripheral blood of patients with solid tumours are of considerable importance for the prognosis and therapeutic correlation. Their detection however is difficult due to lack of sensitivity, specificity and technical problems in standardisation. In this original article we show a new sensitive method overcoming the hitherto known difficulties by combining traditional antibody-techniques with a RT-PCR. Due to this method 2 tumour cells within 5 ml of peripheral blood can be detected in spiking experiments.
Demel
U
U
Jean Dausset Laboratory at Graz University MS, Graz, Austria.
Tilz
G P
GP
Foeldes-Papp
Z
Z
Gutierrez
B
B
Albert
W H
WH
Böcher
O
O
D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D012333:RNA, Messenger; D004492:Edetic Acid