title: Reactive oxygen species generation and peroxidase activity during Oidium neolycopersici infection on Lycopersicon species.
abstract: Histochemical and biochemical study of plant tissue responses were carried out on three Lycopersicon spp. accessions differing in response to Oidium neolycopersici. High production of superoxide anion was observed mainly in infected leaves of highly susceptible Lycopersicon esculentum cv. 'Amateur' during the first hours post inoculation (hpi). The production of hydrogen peroxide as well as an increase of peroxidase (POX) activity were detected mainly in resistant accessions at 4-12 hpi. A signal confirming the presence of very active POX was found in the apical part of tubes of germinating fungus and inside dead conidia. Increased soluble POX and catalase activity in leaf extracts of resistant accessions L. chmielewskii (LA 2663) and L. hirsutum (LA 2128) (20 hpi) correlated with the percentage of dead cells in infection sites. The correlation between production of reactive oxygen species (ROS) and activity of enzymes participating in their metabolism and hypersensitive response was evident during plant defence response.
authors.affiliation: Department of Botany, Faculty of Science, Palacký University, Slechtitelů 11, 783 71 Olomouc, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Mlícková
authors.forename: Lenka
authors.initials: L
authors.lastname: Luhová
authors.forename: Ales
authors.initials: A
authors.lastname: Lebeda
authors.forename: Barbora
authors.initials: B
authors.lastname: Mieslerová
authors.forename: Pavel
authors.initials: P
authors.lastname: Pec
mesh_terms: D001203:Ascomycota / Q000472:pathogenicity*; D018551:Solanum lycopersicum / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology; D010544:Peroxidases / Q000378:metabolism*; D017382:Reactive Oxygen Species
chemical_list: D017382:Reactive Oxygen Species; D010544:Peroxidases