A high-throughput method for identifying N-ethyl-N-nitrosourea (ENU)-induced point mutations in zebrafish.
Draper
Bruce W
BW
Howard Hughes Medical Institute, Seattle, Washington 98109, USA.
McCallum
Claire M
CM
Stout
Jennifer L
JL
Slade
Ann J
AJ
Moens
Cecilia B
CB
D000818:Animals; D020137:Base Pair Mismatch / Q000235:genetics; D015925:Cryopreservation; D004247:DNA / Q000032:analysis; D004252:DNA Mutational Analysis / Q000379:methods*; D004720:Endonucleases / Q000737:chemistry / Q000378:metabolism*; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005787:Gene Frequency; D015723:Gene Library; D008297:Male; D016296:Mutagenesis; D017354:Point Mutation / Q000235:genetics*; D013094:Spermatozoa / Q000737:chemistry; D015027:Zebrafish / Q000235:genetics*
D004247:DNA; D004720:Endonucleases; C406922:CEL I nuclease; D005038:Ethylnitrosourea