Conjugation of antibodies to horseradish peroxidase.
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is purified by gel filtration.
Wisdom
G Brian
GB
School of Biology and Biochemistry, The Queen's University, Belfast, Northern Ireland, UK.
D000818:Animals; D006735:Horseradish Peroxidase / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry*; D009005:Monosaccharides / Q000737:chemistry*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry
D007074:Immunoglobulin G; D009005:Monosaccharides; D012545:Schiff Bases; D010504:Periodic Acid; C009288:metaperiodate; D006735:Horseradish Peroxidase