Phenotypic and functional heterogeneity of porcine blood monocytes and its relation with maturation.
Swine monocytes constitute a heterogeneous population of cells which can be divided into four subsets based on the expression of SWC3, CD14, CD163 and swine leucocyte antigen (SLA) DR markers. These subsets appear to represent different maturation stages in a pathway along which these cells up-regulate the expression of SLA DR and CD163 antigens and reduce that of CD14. Differences in the expression of adhesion and costimulatory molecules are also patent, with a progressive increase in the expression of CD11a, wCD11R1, CD29, CD49d, CD61, CD1a and CD80/86, and a concomitant decrease in that of wCD11R2. Besides, these subsets differ in their capacity for tumour necrosis factor-alpha (TNF-alpha) production in response to lipopolysaccharide + interferon-gamma. The CD163(+) CD14(-) SLA DR(+) subset produces higher amounts of TNF-alpha than the CD163(-) CD14(+) SLA DR(-) subset, whereas CD163(+) CD14(+) SLA DR(+) and CD163(-) CD14(+) SLA DR(+) subsets show intermediate values. CD163(+) monocytes also display a higher ability to present soluble antigens to T cells than CD163(-) monocytes.
Chamorro
Sonia
S
Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña, Km 7,5, 28040 Madrid, Spain.
Revilla
Concepción
C
Alvarez
Belén
B
Alonso
Fernando
F
Ezquerra
Angel
A
Domínguez
Javier
J
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000032:analysis; D013552:Swine / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D000098902:CD163 Antigen
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D015815:Cell Adhesion Molecules; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha