The response of bovine lymphocytes from lymph and blood to phytohaemagglutinin.
Optimal conditions for stimulation of bovine lymphocytes by Phytohaemagglutin (PHA) were established in a microtitre assay. Lymphocytes from efferent duct lymph responded vigorously, with specific incorporation of 100,000 to 200,000 cpm/well, providing the conditions of cell culture are carefully controlled. Peripheral blood lymphocytes responded over a wider range of cultural conditions but optimal specific incorporation was usually only 40-100,000 cpm/well.
Brownlie
J
J
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks, Great Britain.
Stott
E J
EJ
D000818:Animals; D002417:Cattle / Q000097:blood / Q000276:immunology*; D008196:Lymph / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D010835:Phytohemagglutinins