Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.
The predicted amino acid sequence of Bacillus subtilis ybaN (renamed pdaB) exhibits high similarity to those of several polysaccharide deacetylases. Northern hybridization analysis with sporulation sigma mutants indicated that the pdaB gene is transcribed by EsigmaE RNA polymerase and negatively regulated by SpoIIID. The pdaB mutant was deficient in spore formation. Phase- and electron microscopic observation showed morphological changes of spores in late sporulation periods. The pdaB spores that had lost their viability were empty. Moreover, GFP driven by the promoter of the sspE gene was localized in the forespore compartment for the wild type, but was localized in both the mother cell and forespore compartments for phase-gray/dark forespores of the pdaB mutant. This indicates that GFP expressed in the forespores of the mutant leaks into the mother cells. Therefore, PdaB is necessary to maintain spores after the late stage of sporulation.
Fukushima
Tatsuya
T
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan.
Tanabe
Taichi
T
Yamamoto
Hiroki
H
Hosoya
Shigeo
S
Sato
Tsutomu
T
Yoshikawa
Hirofumi
H
Sekiguchi
Junichi
J
D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D015152:Blotting, Northern; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D005816:Genetic Complementation Test; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009154:Mutation; D010848:Picolinic Acids / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013171:Spores, Bacterial / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry
D001426:Bacterial Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D010848:Picolinic Acids; D011134:Polysaccharides; D012333:RNA, Messenger; C071977:SpoIIID protein, Bacillus; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D012321:DNA-Directed RNA Polymerases; D000581:Amidohydrolases; C022427:polysaccharide deacetylase; C004860:dipicolinic acid