title: A rapid microtechnique for in vitro stimulation of canine lymphocytes using whole blood.
abstract: A simple, rapid microtechnique utilizing whole blood has been developed for evaluating the in vitro stimulation of canine lymphocytes. The test uses 5 ul. of heparinized whole blood per culture in a microtiter plate and requires no serum supplement. Cultures are harvested by an automated multiple sample cell harvester. This technique permits large numbers of replicate samples to be tested on individual animals with minimal amounts of blood and minimal technician time.
authors.affiliation: Clinical Immunology Laboratory, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Felsburg
authors.forename: M T
authors.initials: MT
authors.lastname: Reilley
authors.forename: J K
authors.initials: JK
authors.lastname: Sinnigen
mesh_terms: D000818:Animals; D004285:Dogs / Q000276:immunology*; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D010835:Phytohemagglutinins / Q000276:immunology / Q000494:pharmacology*; D010865:Pilot Projects; D011043:Pokeweed Mitogens / Q000276:immunology / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; C028784:phytohemagglutinin-P; D013936:Thymidine