[Effect of tianyuan ketong recipe on the pain behavior, c-fos expression and P substance contents in dorsal horn of spinal cord in formalin induced pain model rats].
OBJECTIVE
To observe the effect and mechanism of Tianyuan Ketong recipe (TKR) on the pain reaction in formalin induced pain model rats.


METHODS
The analgesic effect of TKR was evaluated using pain behavior graded scoring, the c-fos gene expression and P substance contents in superficial lamella of spinal cord dorsal horn in model rats were analyzed by means of immunohistochemical analysis and computer image analysis technique.


RESULTS
TKR could markedly inhibit the pain reaction in model rats (P < 0.05), and the pain induced elevation of c-fos expression and P substance contents could also be suppressed (P < 0.05).


CONCLUSIONS
TKR shows definite analgesic effect on formalin induced pain model rats, the reduction of neuron's reaction in spinal cord dorsal horn to afferent noxious stimulation is possibly one of the pathways for its analgesic effect.
Yang
Ming-hui
MH
Gseneral Hospital of PLA, Beijing (100853). ymh9651@yahoo.com.cn
Zhang
Hai-yan
HY
Liu
Yi
Y
D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005557:Formaldehyde; D007150:Immunohistochemistry; D008297:Male; D016761:Oncogene Proteins v-fos / Q000096:biosynthesis* / Q000235:genetics; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D020671:Posterior Horn Cells / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*
D018712:Analgesics, Non-Narcotic; D004365:Drugs, Chinese Herbal; D016761:Oncogene Proteins v-fos; D005557:Formaldehyde; D013373:Substance P