title: Identification and characterization of human Golgi nucleotide sugar transporter SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family.
abstract: We report the molecular cloning of SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family. The gene SLC35D2 maps to chromosome 9q22.33. SLC35D2 cDNA codes for a hydrophobic protein consisting of 337 amino acid residues with 10 putative transmembrane helices. Northern blot analysis revealed the SLC35D2 mRNA as a single major band corresponding to 2.0 kb in length. SLC35D2 was localized in the Golgi membrane and exhibited around 50% similarity with three nucleotide sugar transporters: human SLC35D1 (UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter), fruitfly fringe connection (frc) transporter, and nematode SQV-7 transporter, the latter two being involved in developmental and organogenetic processes. Heterologous expression of SLC35D2 protein in yeast indicated that UDP-N-acetylglucosamine is a candidate for the substrate(s) of the transporter. The sequence similarity, subcellular localization, and transporting substrate suggest that SLC35D2 is a good candidate for the ortholog of frc transporter, which is involved in the Notch signaling system by providing the fringe N-acetylglucosaminyltransferase with the substrate. We also describe the identification and categorization of the human SLC35 gene family.
authors.affiliation: Department of Biochemical Cell Research, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, 113-8613, Japan. ishidan@rinshoken.or.jp
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ishida
authors.forename: Toshiyasu
authors.initials: T
authors.lastname: Kuba
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Aoki
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Miyatake
authors.forename: Masao
authors.initials: M
authors.lastname: Kawakita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sanai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D006056:Golgi Apparatus / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D033704:Nucleotide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D051880:Receptors, Notch; D012441:Saccharomyces cerevisiae; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D033704:Nucleotide Transport Proteins; D051880:Receptors, Notch; C487823:SLC35D2 protein, human