Sympathetic vesicovascular reflex induced by acute urinary retention evokes proinflammatory and proapoptotic injury in rat liver.
Increased hepatic sympathetic activity affects hepatic metabolism and hemodynamics and subsequently causes acute hepatic injury. We examined whether the vesicovascular reflex evoked by bladder overdistension could affect hepatic function, specifically reactive oxygen species (ROS)-induced inflammation and apoptosis, through activation of the hepatic sympathetic nerve. We evaluated the hepatic hemodynamics, hepatic sympathetic nervous activities, and cystometrograms in anesthetized rats subjected to acute urinary retention. We used a chemiluminescence method, an in situ nitro blue tetrazolium perfusion technique, and a DNA fragmentation/apoptosis-related protein assay to demonstrate de novo and colocalize superoxide production and apoptosis formation in rat liver. Acute urinary retention increased the hepatic sympathetic-dependent vesicovascular reflex, which caused hepatic vasoconstriction/hypoxia and increased superoxide anion production from the periportal Kupffer cells and hepatocytes, which were aggravated by the increase in volume and duration of urinary retention. The ROS-enhanced proinflammatory NF-kappaB, activator protein-1, and ICAM-1 expression also promoted proapoptotic mechanisms, including increases in the Bax/Bcl-2 ratio, CPP32 expression, poly-(ADP-ribose)-polymerase cleavages, and DNA fragmentation and apoptotic cells in the liver. The proinflammatory and proapoptotic mechanisms were significantly attenuated in rats treated with hepatic sympathetic nerve denervation or catechin (antioxidant) supplement. In conclusion, our results suggest that acute urine retention enhances hepatic sympathetic activity, which causes hepatic vasoconstriction and evokes proinflammatory and proapoptotic oxidative injury in the rat liver. Reduction of the hepatic sympathetic tone or antioxidant supplement significantly attenuates these injuries.
Yu
Hong-Jeng
HJ
Department of Urology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Lin
Bor-Ru
BR
Lee
Hsuan-Su
HS
Shun
Chia-Tung
CT
Yang
Chih-Ching
CC
Lai
Ting-Yu
TY
Chien
Chiang-Ting
CT
Hsu
Su-Ming
SM
D000208:Acute Disease; D000447:Aldehydes; D000818:Animals; D001646:Bile / Q000378:metabolism; D053938:DNA Fragmentation; D005260:Female; D015870:Gene Expression; D006505:Hepatitis / Q000209:etiology* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008099:Liver / Q000378:metabolism / Q000473:pathology; D009580:Nitroblue Tetrazolium; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012018:Reflex / Q000502:physiology*; D013194:Staining and Labeling; D013564:Sympathetic Nervous System / Q000502:physiology*; D001743:Urinary Bladder / Q000294:innervation / Q000502:physiology; D016055:Urinary Retention / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology*
D000447:Aldehydes; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1; D009580:Nitroblue Tetrazolium; C027576:4-hydroxy-2-nonenal