title: Elevated voltage gated Cl- channel expression enhances fast paired-pulse inhibition in the dentate gyrus of seizure sensitive gerbil.
abstract: The present study was performed to determine whether the voltage gated Cl- channel (CLC) expression is altered in the hippocampus of seizure sensitive (SS) gerbils, and to identify the strong fast paired-pulse inhibition in the dentate gyrus of SS gerbils is associated with altered CLC expression. In the hippocampal proper and the granule cell layer of the dentate gyrus of the SS gerbils, strong CLC-2 immunoreactivity was detected, as compared with seizure resistant (SR) gerbils. In addition, CLC-3 immunoreactivity was observed in the CA1-3 pyramidal cells, and the granule cell and the molecular layer of the dentate gyrus in the SS gerbils, whereas its immunoreactivity was rarely detected in the SR gerbils. However, CLC-3 immunoreactivity in the mossy fiber was reduced, as compared with SR gerbils. Moreover, infusion of the potential CLC inhibitor (4,4'-diisothiocyanostibene-2,2'-disulfanic acid, DIDS) reduced fast paired-pulse inhibition in the dentate gyrus of SS gerbils, although evoked responses in the dentate gyrus between SR and SS gerbils were similarly detected. These findings suggest that enhancement of CLC expression in the dentate gyrus of SS gerbils may be one of the compensatory responses for reduced GABA(A) receptor-mediated fast postsynaptic inhibitory potentials.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702, South Korea.
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: In-Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Je Hoon
authors.initials: JH
authors.lastname: Seo
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000200:Action Potentials; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D015153:Blotting, Western / Q000379:methods; D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000145:classification / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology; D018891:Dentate Gyrus / Q000378:metabolism* / Q000503:physiopathology; D004357:Drug Synergism; D005849:Gerbillinae; D007150:Immunohistochemistry / Q000379:methods; D009433:Neural Inhibition / Q000502:physiology*; D012640:Seizures / Q000503:physiopathology*
chemical_list: D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels; C115376:ClC-3 channel; D003292:Convulsants; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D001640:Bicuculline