Induction of chimerism and tolerance using freshly purified or cultured hematopoietic stem cells in nonmyeloablated mice.
The development of protocols to induce a state of durable mixed allogeneic hematopoietic chimerism to confer robust donor-specific transplant tolerance has been a major focus of the transplant community for the past decade. High levels of mixed allogeneic hematopoietic chimerism across a full major histocompatibility complex (MHC) barrier can be achieved by total myeloablation and transfusion of host and donor bone-marrow cells or, as shown more recently, can be achieved with nonmyeloablative preconditioning regimes in combination with donor bone-marrow transfusions (2-4) or transfusion of purified hematopoietic stem cells (HSC) (5-7). Here we illustrate useful experimental techniques for the study of hematopoietic chimerism in rodents by describing a system in which chimerism can be induced using nonmyeloablative conditioning, short-term costimulation blockade, and transplantation of purified or cultured hematopoietic stem cells.
Emmanouilidis
Nikos
N
Department of Abdominal and Transplant Surgery, Hannover Medical School, Hannover, Germany.
Larsen
Christian P
CP
D000818:Animals; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D002678:Chimera / Q000276:immunology*; D046528:Chimerism; D016895:Culture Media, Serum-Free; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D008297:Male; D051379:Mice; D014180:Transplantation
D016895:Culture Media, Serum-Free