title: Crystalization and preliminary X-ray crystallographic analysis of the laminarinase endo-beta-1,3-glucanase from Pyrococcus furiosus.
abstract: Laminarinase endo-beta-1,3 glucanase (LamA) from Pyrococcus furiosus is an enzyme which displays its main hydrolytic activity on the 3-1,3-glucose polymer laminarin. This laminarinase is remarkably resistant to denaturation: its secondary structure is unchanged in 8 M guanidinium chloride. This protein belongs to the family 16 glycosyl hydrolases, which are enzymes that are widely distributed among bacteria, fungi and higher plants. Single crystals of P. furiosus LamAhave been obtained by the hanging-drop vapour-diffusion method using 2-methyl-2,4-pentanediol as a precipitant agent. A complete data set has been collected under cryocooling at a synchrotron source. The crystals belong to the monoclinic space group P21, with unit-cell parameters a = 44.36, b = 84.76, c = 69.23 A, a = 90, fl = 104.97, y = 90 degrees, and diffract to 2.15 A resolution.
authors.affiliation: Instituto di Biologia e Patologia Molecolari del CNR, Roma, Italy. andrea.ilari@uniroma.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Ilari
authors.forename: Sebastiana
authors.initials: S
authors.lastname: Angelaccio
authors.forename: Annarita
authors.initials: A
authors.lastname: Fiorillo
authors.forename: Rita
authors.initials: R
authors.lastname: Florio
authors.forename: Valerio
authors.initials: V
authors.lastname: Consalvi
authors.forename: Roberta
authors.initials: R
authors.lastname: Chiaraluce
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry*; D044602:Cellulases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D006018:Glycols / Q000737:chemistry; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D020027:Pyrococcus furiosus / Q000201:enzymology*; D017356:Synchrotrons; D013696:Temperature
chemical_list: D019843:Archaeal Proteins; D006018:Glycols; D044602:Cellulases; D002480:Cellulase; C011480:hexylene glycol