Characterization of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis in Brazil.
In this study the nucleotide sequence of the pncA gene from 59 Mycobacterium tuberculosis clinical isolates was analyzed. Mutations in the pncA gene were identified in 29 of 40 pyrazinamide-resistant isolates, and no pyrazinamidase activity was detected in 39 of them. Twelve mutations found in this work have not been described previously.
Rodrigues
Vívian de F Sumnienski
Vde F
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Rua Bento Gonçalves 9500, Prédio 43421, CEP 91501-970 Porto Alegre, RS, Brazil.
Telles
Maria Alice
MA
Ribeiro
Marta Osório
MO
Cafrune
Patrícia Izquierdo
PI
Rossetti
Maria Lucia Rosa
ML
Zaha
Arnaldo
A
D000581:Amidohydrolases / Q000235:genetics*; D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D011718:Pyrazinamide / Q000494:pharmacology*; D014376:Tuberculosis / Q000382:microbiology
D000995:Antitubercular Agents; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis