title: Establishment and characterization of four canine melanoma cell lines.
abstract: Four canine melanoma cell lines were established from the subcutaneous, oral gingival and mucosal melanoma tissues at the primary and metastatic sites. These cell lines were designated as CMeC-1, CMeC-2, KMeC and LMeC. The cells were spindles in shape, similar to that of primary tumor cells. The doubling times of these cells ranged from 34.1 +/- 5.61 to 57.9 +/- 3.28 hr and their chromosome number ranged from 46 to 80. When transplanted into nude mice, CMeC-1 and LMeC produced tumors, whereas CMeC-2 and KMeC did not. The morphology of the tissue formed by xenotransplantation of these cells was similar to their primary tumors.
authors.affiliation: Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan
authors.forename: Kaori
authors.initials: K
authors.lastname: Inoue
authors.forename: Emi
authors.initials: E
authors.lastname: Ohashi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kadosawa
authors.forename: Sung-Hyeok
authors.initials: SH
authors.lastname: Hong
authors.forename: Satoru
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000473:pathology*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D008297:Male; D008545:Melanoma / Q000473:pathology / Q000556:secondary / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009062:Mouth Neoplasms / Q000662:veterinary; D009368:Neoplasm Transplantation; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000662:veterinary; D014183:Transplantation, Heterologous