Organ microcirculatory disturbances in experimental acute pancreatitis. A role of nitric oxide.
Microcirculatory disturbances are important early pathophysiological events in various organs during acute pancreatitis (AP). The aim of the study was to investigate an influence of L-arginine (nitric oxide substrate) and N(G)-nitro-L-arginine (L-NNA, nitric oxide synthase inhibitor) on organ microcirculation in experimental acute pancreatitis induced by four consecutive intraperitoneal cerulein injections (15 microg/kg/h). The microcirculation of pancreas, liver, kidney, stomach, colon and skeletal muscle was measured by laser Doppler flowmeter. Serum interleukin 6 and hematocrit levels were analyzed. AP resulted in a significant drop of microperfusion in all examined organ. L-arginine administration (2 x 100 mg/kg) improved the microcirculation in the pancreas, liver, kidney, colon and skeletal muscle, and lowered hematocrit levels. L-NNA treatment (2 x 25 mg/kg) caused aggravation of edematous AP to the necrotizing situation, and increased IL-6 and hematocrit levels. A further reduction of blood perfusion was noted in the stomach only. It is concluded that L-arginine administration has a positive influence on organ microcirculatory disturbances accompanying experimental cerulein-induced AP. NO inhibition aggravates the course of pancreatitis.
Dobosz
M
M
Department of General and Gastroenterological Surgery, St. Vincent a'Paulo Hospital, Gdynia, Poland. marek-dobosz@wp.pl
Hac
S
S
Mionskowska
L
L
Dymecki
D
D
Dobrowolski
S
S
Wajda
Z
Z
D000208:Acute Disease; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002108:Ceruletide; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D008833:Microcirculation / Q000502:physiology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D010179:Pancreas / Q000098:blood supply; D010195:Pancreatitis / Q000139:chemically induced / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology
D004791:Enzyme Inhibitors; D015850:Interleukin-6; D019335:Nitroarginine; D009569:Nitric Oxide; D002108:Ceruletide; D001120:Arginine; D019001:Nitric Oxide Synthase