Use of a monoclonal antibody against Babesia bovis merozoite surface antigen-2c for the development of a competitive ELISA test.
Bovine babesiosis caused by Babesia bovis is a disease that hampers the production of beef and dairy cattle in tropical and subtropical regions of the world. New diagnostic methods based on recombinant antigens constitute valuable biotechnological tools for the strategic control of this disease. We have developed a competitive enzyme-linked immunosorbent assay that includes a recombinant form of the merozoite surface antigen-2c and a novel monoclonal antibody against it. Preliminary results showed that this test is able to identify specific antibodies against B. bovis from experimentally and naturally infected cattle.
Dominguez
Mariana
M
Instituto Nacional de Tecnología Agropecuaria, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Castelar, Argentina.
Zabal
Osvaldo
O
Wilkowsky
Silvina
S
Echaide
Ignacio
I
Torioni de Echaide
Susana
S
Asenzo
Gustavo
G
Rodríguez
Anabel
A
Zamorano
Patricia
P
Farber
Marisa
M
Suarez
Carlos
C
Florin-Christensen
Monica
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology*; D016793:Babesia bovis / Q000276:immunology* / Q000472:pathogenicity*; D001404:Babesiosis / Q000175:diagnosis* / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology*; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; C476548:MSA-2 protein, Babesia bovis; D008565:Membrane Proteins; D015800:Protozoan Proteins