title: A functional truncated form of c-kit tyrosine kinase is produced specifically in the testis of the mouse but not the rat, pig, or human.
abstract: The complete nucleotide sequence of mouse-truncated mRNA of c-kit, tr-kit, has been determined using the CD1 strain. In this study, the nucleotide sequences of tr-kit from AKR/N, C57BL/6, and ICR strains of mice were determined and found to be identical, although many silent variations were found compared with the sequence in a database for CD1. Tr-kit protein consists of 12 amino acids encoded by the 16th intron and the following 190 amino acids of c-kit. In the sequences of tr-kit encoding 12 specific amino acids, no substitution was detected among the three strains and CD1. Furthermore, RT-PCR analysis clearly showed that tr-kit mRNA expression was present only in testis. No nucleotide mutation in two important regions of the presumptive promoter for tr-kit mRNA was detected within the 16th intron of the mouse strains examined. However, no functional form of tr-kit was found in the rat, pig, or human by sequence analysis and homology testing.
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Sakamoto
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yoneda
authors.forename: Kei
authors.initials: K
authors.lastname: Terada
authors.forename: Yuka
authors.initials: Y
authors.lastname: Namiki
authors.forename: Keita
authors.initials: K
authors.lastname: Suzuki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Mori
authors.forename: Junji
authors.initials: J
authors.lastname: Ueda
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D013045:Species Specificity; D013552:Swine; D013737:Testis / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D019009:Proto-Oncogene Proteins c-kit