title: Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides.
abstract: We examined expression and function of osteopontin (OPN) in oral cancer cell lines using antisense oligonucleotide (AS). Quantitative real-time RT-PCR showed that expression in BSC-OF cells was significantly higher (10-fold) than that in KB cell. AS-study showed that foci of AS-treated BSC-OF cells possessed thin processes and radiated morphologically, although BSC-OF cells showed round foci. Cell growth in AS-group was lower (<80%) than the control. Invasion ability in AS-group became significantly lower (P<0.01). These results suggest that BSC-OF cell is useful for over-expression of OPN, and that OPN contributes to morphology, growth and invasion.
authors.affiliation: Department of Pathology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, Japan. tmuramat@tdc.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Muramatsu
authors.forename: Kaori
authors.initials: K
authors.lastname: Shima
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Ohta
authors.forename: Harutoshi
authors.initials: H
authors.lastname: Kizaki
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Ro
authors.forename: Yohko
authors.initials: Y
authors.lastname: Kohno
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Abiko
authors.forename: Masaki
authors.initials: M
authors.lastname: Shimono
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D016376:Oligonucleotides, Antisense; D053495:Osteopontin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D016376:Oligonucleotides, Antisense; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin