title: The significance of oxidant/antioxidant balance for the pathogenesis of experimental sepsis by multidrug-resistant Pseudomonas aeruginosa.
abstract: OBJECTIVE
  The significance of lipid peroxidation as an independent factor leading to sepsis by multidrug-resistant Pseudomonas aeruginosa. Design experimental study.
  
  
  METHODS
  Twenty-six rabbits were applied. They were divided into two groups; A (n=6) comprising controls, and B (n=20) comprising animals infected by the injection of 1x10(8) cfu/kg inoculum of the test pathogen into the left inner jugular vein. Six rabbits of group B were followed-up to estimate survival; all of the remaining were sacrificed. Blood was sampled for the determination of serum malondialdehyde (MDA) by the thiobarbiturate assay, total antioxidant status (TAS) by a chromogenic assay, tumor necrosis factor alpha by a bioassay on fibrosarcoma L929 cell line, and endotoxins (LPS) by the QCL-1000 LAL assay.
  
  
  RESULTS
  Mean survival of group B was 60.0+/-15.8 h. MDA was significantly higher in group B compared to group A at 30, 60, 120 and 150 min. TAS was statistically decreased in group B compared to group A at 30 and 60 min. Increases of MDA in group B were followed by reciprocal decreases of TAS (P of correlation <0.001). Hemodynamic instability was recorded in group B compared to group A 160 min after bacterial challenge.
  
  
  CONCLUSIONS
  Early alterations of oxidant/antioxidant balance occur in experimental sepsis by multidrug-resistant P. aeruginosa followed by hemodynamic instability. Results highlight the perspective of the administration of antioxidants as immunomodulatory treatment of sepsis in animal studies.
authors.affiliation: Department of Biological Chemistry, Medical School, University of Athens, Greece.
authors.forename: Vassilios
authors.initials: V
authors.lastname: Koussoulas
authors.forename: Evangelos J
authors.initials: EJ
authors.lastname: Giamarellos-Bourboulis
authors.forename: Theodoros
authors.initials: T
authors.lastname: Adamis
authors.forename: Maria
authors.initials: M
authors.lastname: Mouktaroudi
authors.forename: Lambros
authors.initials: L
authors.lastname: Sabracos
authors.forename: Despina
authors.initials: D
authors.lastname: Perrea
authors.forename: Helen
authors.initials: H
authors.lastname: Giamarellou
authors.forename: Amalia
authors.initials: A
authors.lastname: Dionyssiou-Asteriou
mesh_terms: D000818:Animals; D024901:Drug Resistance, Multiple, Bacterial; D015227:Lipid Peroxidation; D008070:Lipopolysaccharides / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D011552:Pseudomonas Infections / Q000097:blood / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000472:pathogenicity*; D011817:Rabbits; D018805:Sepsis / Q000097:blood / Q000382:microbiology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde