Comparison of gamma- and UV-light-induced EPR spectra of enamel from deciduous molar teeth.
From previous work, it is known that CO2- radicals in tooth enamel are induced by gamma as well as by UV-light exposure. The parameters of the EPR signal of the CO2- radical were found to be independent of the source of exposure. However, it would be desirable for retrospective dosimetry to identify other characteristic features of the EPR spectrum of tooth enamel, which would allow differentiation between the two sources of exposure. In the present work, enamel of deciduous molars was exposed to gamma-radiation from a 60Co-source and 254 nm UV-light from a low-pressure mercury lamp. The resulting EPR spectra were deconvoluted, and the native spectrum simulated from spectra of the CO2- radical, and two further EPR lines. Both EPR signals of the native spectrum were located at g=2.0046, but were different in line shape and width. One was a 1 mT wide isotropic signal of Gaussian line shape while the other was a 0.7 mT wide axial signal of Lorentzian line shape. A comparable study of the amplitudes of the native and CO2- signals was done before and after gamma- and UV-light exposure. While the native signals were found to be only slightly sensitive to gamma-radiation, their amplitude increased significantly on UV-light exposure. Feasibilities are discussed to distinguish different radiation sources by exposure-induced alterations of the native EPR spectrum.
El-Faramawy
N A
NA
GSF-National Research Centre for Environment and Health, Institute for Radiation Protection, D-85758 Neuherberg, Germany. na_bil@yahoo.com
D001822:Body Burden; D002648:Child; D003743:Dental Enamel / Q000737:chemistry* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008963:Molar / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods*; D012062:Relative Biological Effectiveness; D015203:Reproducibility of Results; D018570:Risk Assessment / Q000379:methods; D012680:Sensitivity and Specificity; D014094:Tooth, Deciduous / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays