[Effects of catalase on the mitochondria and apoptosis of SW480 cells].
OBJECTIVE
To investigate the effects of catalase on the mitochondria and apoptotic behavior of SW480 cells treated by lipopolysaccharide (LPS), and the changes in intracellular expression of nuclear factor kappaB (NF-kappaB).


METHODS
LPS-stimulated SW480 cells were treated with catalase and the changes in their mitochondria and apoptotic behavior were observed by transmission electron microscopy. The expression of NF-kappaB was detected by immunohistochemical method.


RESULTS
Mitochondria damages and apoptosis were slightly diminished in the LPS-stimulated cells with catalase pretreatment, and the NF-kappaB expression was also decreased.


CONCLUSIONS
Catalase protect the mitochondria of SW480 cells from damages by LPS and inhibit the cell apoptosis, possibly due to the activation of NF-kappaB.
Wang
Qun-ying
QY
Institute for Digestive Diseases of PLA, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. captainwl@163.com
Wang
Ji-de
JD
Zhong
Shi-shun
SS
Wang
Ya-dong
YD
Sun
Yong
Y
Li
Liang-ren
LR
Zhang
Ya-li
YL
D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008070:Lipopolysaccharides; D008928:Mitochondria / Q000378:metabolism*; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics
D008070:Lipopolysaccharides; D016328:NF-kappa B; D002374:Catalase