Comparison of the pharmacokinetics of lidamycin in mice determined by two methods.
OBJECTIVE
To compare two methods, the total radioactivity assay (RA method) and the radioactivity assay after separation with high performance liquid chromatography (HPLC-RA method).


METHODS
125I-Lidamycin was prepared by Iodogen method and separated by size exclusive high performance liquid chromatography. The pharmacokinetic parameters of lidamycin were assayed by two methods after intravenous injection to mice at the dose of 100 microg x kg(-1), and compared by statistical analysis.


RESULTS
The pharmacokinetic parameters (Vd, T1/2alpha, T1/2beta, K21, K10, K12, AUC and CL) showed significant difference between the two methods (P < 0.05).


CONCLUSIONS
The HPLC-RA method was better than the RA method to determine unchanged 125I-lidamycin.
Liu
You-ping
YP
National Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China.
Li
Quan-sheng
QS
Huang
Yu-rong
YR
Liu
Chang-xiao
CX
D000617:Aminoglycosides / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000097:blood / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D053281:Enediynes; D005260:Female; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D012680:Sensitivity and Specificity
D000617:Aminoglycosides; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D007457:Iodine Radioisotopes; C058024:C 1027