Cryopreservation of isolated mouse germinal vesicles.
The storage of unfertilized oocytes, either immature, maturing or mature, is still unsatisfactory. Here we describe an approach in which germinal vesicles isolated as karyoplasts from immature oocytes are vitrified by open the pulled straws (OPS) method in evacuated porcine zonae pellucidae. After thawing, their survival was almost absolute. Moreover, when thawed GV-karyoplasts were fused to immature oocyte cytoplasts the maturation of reconstructed cells resulted in the production of secondary oocytes--metaphase II.
Kren
Radomir
R
Institute of Animal Production, Prague, Czech Republic. kren@vuzv.cz
Fulka
Josef
J
Fulka
Helena
H
D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology*; D013552:Swine; D015044:Zona Pellucida