Method for determination of (-102C>T) single nucleotide polymorphism in the human manganese superoxide dismutase promoter.
BACKGROUND
Manganese superoxide dismutase (MnSOD) plays a critical role in the detoxification of mitochondrial reactive oxygen species constituting a major cellular defense mechanism against agents that induce oxidative stress. The MnSOD promoter contains an activator protein-2 (AP-2) binding site that modifies transcription of MnSOD. Mutations have been identified in the proximal region of the promoter in human tumor cell lines. One of these mutations (-102C>T) has been shown to change the binding pattern of AP-2 leading to a reduction in transcriptional activity. The aim of our study was to develop a method to identify and determine the frequency of this (-102C>T) polymorphism in human tissues.


RESULTS
A new TaqMan allelic discrimination genotype method was successfully applied to genomic DNA samples derived from blood, buccal swabs, snap frozen tissue and paraffin blocks. The polymorphism was shown to be in Hardy-Weinberg Equilibrium in an evaluation of 130 Caucasians from Warsaw, Poland: 44 (33.8%) were heterozygous and 6 (4.6%) were homozygous for -102T.


CONCLUSIONS
This report represents the first description of the MnSOD -102C>T polymorphism in human subjects by a novel Taqman allelic discrimination assay. This method should enable molecular epidemiological studies to evaluate possible associations of this polymorphism with malignancies and other diseases related to reactive oxygen species.
Martin
Robert C G
RC
Department of Surgery, University of Louisville School of Medicine, Louisville, KY, USA. robert.martin@louisville.edu
Hughes
Kalista
K
Doll
Mark A
MA
Lan
Qing
Q
Martini
Benjamin D
BD
Lissowska
Jolanta
J
Rothman
Nathaniel
N
Hein
David W
DW
D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics*; D050656:Transcription Factor AP-2 / Q000378:metabolism
D015342:DNA Probes; D005456:Fluorescent Dyes; D050656:Transcription Factor AP-2; D004247:DNA; D013482:Superoxide Dismutase