Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase.
Rhodococcus pyridinovorans MW3 was isolated from an arable land of manioc from the Congo for its ability to transform acrylonitrile to acrylamide. This strain contains a cobalt nitrile hydratase (NHase) showing high sequence homology with NHases so far described. The specific NHase activity was 97 U mg(-1) dry wt. NHase production by R. pyridinovorans MW3 was urea and Co-dependent. The NHase was active for acrylamide up to 60% (w/v) indicating its potential for acrylamide production.
Precigou
Sylvain
S
Laboratoire d'Ecologie Moléculaire, IBEAS, EA 3525, Université de Pau et des Pays de l'Adour, avenue de l'Université, UFR Sciences et Techniques, BP 1155 F-64013, Pau cedex, France.
Wieser
Marco
M
Pommares
Patrick
P
Goulas
Philippe
P
Duran
Robert
R
D020106:Acrylamide / Q000737:chemistry*; D000181:Acrylonitrile / Q000737:chemistry*; D000595:Amino Acid Sequence; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics; D012988:Soil Microbiology
D012336:RNA, Ribosomal, 16S; D020106:Acrylamide; D006836:Hydro-Lyases; C048831:nitrile hydratase; D000181:Acrylonitrile