[Hepatitis B virus DNA detection by means of polymerase chain reaction in patients with chronic hepatitis and hepatocellular carcinoma].
HBV immunological detection and HBV DNA detection by polymerse chain reaction (PCR) or molecular hybridization with 32P labeled probe were done in 61 patients with chronic hepatitis (CH) and 47 patients with hepatocellular carcinoma (HCC) to investigate the relationship between HBV replication and serum markers. The HBV DNA was detected in 90.50% of CH patients and 50.00% of HCC patients with HBsAg, HBeAg and anti-HBc positive; in 45.40% of CH patients and 7.14% of HCC patients with HBsAg, anti-HBe and anti-HBc positive; in 60.00% of CH patients and 40.00% of HCC patients with HBsAg and negative HBeAg, anti-HBe positive; in 20.00% of CH patients and 22.22% of HCC patients with HBsAg negative and anti-HBe or anti-HBe or anti-HBs positive; in 0 of CH or HCC patients lack of HBV serum markers. Our data suggested that the most active HBV replication was correlated to the presence of both HBsAg and HBeAg, there were some extent of HBV replication in CH or HCC patients with positive HBsAg, the inhibition of HBV replication was related to anti-HBe, the less active HBV replication was seen in HCC patients when compared with CH patients.
Song
Y
Y
Department of Microbiology, Second Military Medical University, Shanghai 200433.
Pan
W
W
Fan
Z
Z
Du
P
P
Qi
Z
Z
D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000821:virology*; D004279:DNA, Viral / Q000097:blood / Q000235:genetics*; D006510:Hepatitis B Antibodies / Q000097:blood; D006511:Hepatitis B Antigens / Q000097:blood / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006521:Hepatitis, Chronic / Q000276:immunology / Q000821:virology*; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens