[Establishment of computer-based video-tracking conditioned place preference experiment system in mice].
To establish the computer-based video-tracking conditioned place preference (CPP) system in mice.



The CPP system was composed of computer, camera, soundproof box, shuttle box and analytical software. The results of morphine-induced conditioned place preference were used to evaluate the experiment system. And the effect of morphine-induced locomotor activity in drug-paired compartment was studied in mice.



Low (1 mg/kg, i.p.), moderate (3 mg/kg, 5 mg/kg, i.p.) and high (10 mg/kg, i.p.) dose of morphine significantly prolonged the time mice spent in drug-paired compartment compared with saline, but there was no dose-response relation. Moderate and high dose of morphine significantly enhanced locomotor activity, among which 5 mg/kg and 10 mg/kg morphine induced behavior sensitization in drug-paired compartment during the conditioning sessions.



The computer-based video-tracking conditioned place preference experiment system in mice established successfully is reliable and stable.
Long
Zai-hao
ZH
Department of Toxicology, College of Medicine, Zhejiang University, Hangzhou 310006, China.
Zhu
Yong-ping
YP
Chen
Hui-hong
HH
Zhou
Lian-fang
LF
D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects* / Q000502:physiology; D001330:Electronic Data Processing; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D009020:Morphine / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects*; D009294:Narcotics / Q000494:pharmacology; D009293:Opioid-Related Disorders / Q000503:physiopathology / Q000523:psychology; D014741:Video Recording
D009294:Narcotics; D009020:Morphine