[Effects of dexamethasone and vitamin B12 on EGF, TGFalpha, and TGFbeta1 gene expressions of A/J mouse palatal cells in vitro].
OBJECTIVE
To investigate the molecular mechanism of congenital cleft palate induced by Dexamethasone (DEX) and the counteractive effect of Vitamin B12.


METHODS
The RT-PCR was used to detect the independent and joint effects of DEX and Vitamin B12 on the gene expressions of A/J embryonic mouse palatal cells.


RESULTS
DEX stimulated the gene expressions of EGF and TGFbeta1, while the expression of TGFalpha left unchanged in the palatal cells. Vitamin B12 had no direct effects on the gene expressions of EGF, TGFalpha, and TGFbeta1; however, it counteracted the effects of DEX on the palate cells.


CONCLUSIONS
DEX induces cleft palate through increasing some gene expressions of growth factors, which can be inhibited by Vitamin B12.
Shi
Bing
B
Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Sun
Jinhu
J
Wang
Dazhang
D
Zuo
Hui
H
D000818:Animals; D002478:Cells, Cultured; D002972:Cleft Palate / Q000139:chemically induced; D003907:Dexamethasone / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D010159:Palate / Q000166:cytology*; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis* / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D014805:Vitamin B 12 / Q000494:pharmacology*
D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D003907:Dexamethasone; D014805:Vitamin B 12