[The effects of traditional Chinese medicine on distribution of F-actin of leukocytes in acute edematous pancreatitis].
OBJECTIVE
To explore the distribution of F-actin of leukocytes in local circulation of pancreas and system circulation and assess the effects of traditional Chinese medicine WPY complex there on in the case of acute edematous pancreatitis(AEP).


METHODS
AEP model of Wistar rats was induced with subcutaneous injection of cerulein. In the treatment group, rats were given 50% WPY (1.5 ml every time) at 0, 2, 4, 6 and 8 hours during the experiment. The leukocytes of blood from the splenic vein and inferior vena cava were incubated with TRITC-Phalloidin for F-actin, and the images were taken with confocal laser scanning microscope. The The distribution of F-actin at the border or central part of leukocyte was analyzed by using Mias system. Results ratio of F-actin border/center gray value of leukocytes increased at the 2nd, 4th and 6th hour, then declined at the 8th hour in AEP rats, but the ratio of F-actin border/center gray value of leukocytes in the blood from the inferior vena cava declined significantly at the 6th hour in the WPY-treated AEP rats(P < 0.01). At 6 hours following the onset of experimental acute edematous pancreatitis, F-actin polymerization and redistribution in the border region of leukocytes were prominent.


CONCLUSIONS
These evidences indicate that leukocytes play an important role in the development of acute pancreatitis. Traditional Chinese medicine WPY can regulate actin redistribution of leukocytes; moreover, WPY may attenuate the stiffness of leukocytes and render them more flexible and transformable in microcirculation.
Tang
Shibin
S
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Zhaoda
Z
Jiang
Junming
J
Zhou
Zongguang
Z
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002108:Ceruletide; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012250:Rheum / Q000737:chemistry
D000199:Actins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D002108:Ceruletide