High-resolution chromosome arm 5p array CGH analysis of small cell lung carcinoma cell lines.
Genomic amplification of regions on chromosome arm 5p has been observed frequently in small cell lung cancer (SCLC), implying the presence of multiple oncogenes on this arm. Although conventional comparative genomic hybridization (CGH) detects gross chromosomal copy number changes, gene discovery requires a higher-resolution approach in order to identify regions of alteration precisely. To identify candidate genes on this chromosome arm, we developed a high-resolution, 10-clone-per-megabase bacterial artificial chromosome CGH array for 5p and examined a panel of 15 SCLC cell lines. Utilization of this CGH array has allowed the fine-mapping of breakpoints to regions as small as 200 kb in a single experiment. In addition to reporting our observations of aberrations at the well-characterized SKP2 and TERT loci, we describe the identification of microdeletions that have escaped detection by conventional screens and the identification TRIO and ANKH as novel putative oncogenes.
Coe
Bradley P
BP
British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada. bcoe@bccrc.ca
Henderson
Laura-Jane
LJ
Garnis
Cathie
C
Tsao
Ming-Sound
MS
Gazdar
Adi F
AF
Minna
John
J
Lam
Stephen
S
Macaulay
Calum
C
Lam
Wan L
WL
D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D002895:Chromosomes, Human, Pair 5 / Q000648:ultrastructure; D004247:DNA / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D004247:DNA