title: [Detection of a cultivated hyperthermophilic archaeon of the genus Sulfophobococcus in a methane tank operated in a thermophilic regime].
abstract: A stable association of hyperthermophilic microorganisms (82 degrees C), which contained mostly cocci and a minor amount of non-spore-forming rods, was obtained from the fermented sludge of a methane tank used to process municipal wastewaters under thermophilic conditions (50 degrees C). PCR amplification of 16S rRNA genes using total DNA isolated from this association and archaea-specific primers, followed by sequencing of the product obtained, showed that the archaeal component was represented by a single nucleotide sequence, which was 99.9% homologous to 16S rRNA gene of Sulfophobococcus zilligii. Thus, a hyperthermophilic archaeon was for the first time detected in a system of anaerobic biological treatment of wastewater. In addition, this is the first report on the detection of a cultivated member of Crenarchaeota in anthropogenic habitats with neutral pH.
authors.forename: G B
authors.initials: GB
authors.lastname: Slobodkina
authors.forename: A I
authors.initials: AI
authors.lastname: Slobodkin
authors.forename: T P
authors.initials: TP
authors.lastname: Turova
authors.forename: N A
authors.initials: NA
authors.lastname: Kostrikina
authors.forename: E A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaia
mesh_terms: D001673:Biodegradation, Environmental; D019615:Crenarchaeota / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D019766:Genes, Archaeal; D008697:Methane / Q000378:metabolism; D016133:Polymerase Chain Reaction; D019642:RNA, Archaeal / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013696:Temperature
chemical_list: D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008697:Methane