Identification of novel tick salivary gland proteins for vaccine development.
Methods currently used to control Ixodes scapularis ticks rely principally on acaricidal applications which suffer from a number of limitations. Recently, host vaccination against ticks has been shown to be a promising alternative tick control method. In tick salivary glands, numerous genes are induced during the feeding process. Many of these newly expressed proteins are secreted in tick saliva and may play a role in modulating host immune responses and pathogen transmission. We have performed suppression subtraction hybridization to identify unique I. scapularis salary gland proteins specifically expressed during engorgement. We have cloned and sequenced ten unique salivary gland-associated cDNAs that are up-regulated during feeding. The protein products of these genes represent potential vaccine candidates for use in the control of ticks and to prevent transmission of tick-borne diseases.
Xu
Yun
Y
Department of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
Bruno
John F
JF
Luft
Benjamin J
BJ
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015195:Drug Design; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D018884:Ixodes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017282:Tick-Borne Diseases / Q000276:immunology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology / Q000627:therapeutic use
D012471:Salivary Proteins and Peptides; D014614:Vaccines, Synthetic