Species identification of poultry egg products.
Species-specific primers for duck were deduced from the mitochondrial ATPase8 gene sequence. Species-specific PCR for turkeys and ducks showed no cross reaction with mixtures from chicken and guinea eggs and detection was possible to a concentration of 0.1% of homogenized duck egg and 5% of homogenized turkey egg when a PCR of 35 cycles was applied. A PCR of 30 cycles detected 10% of homogenized duck egg. The same sensitivities were obtained in dilutions of homogenized egg yolk; however, no PCR signals were obtained in egg white. Analysis of 13 industrial egg product samples showed the practical relevance of the species-specific PCR tests described herein.
Herman
L
L
Ministry of the Flemish Community, Centre of Agricultural Research, Department of Animal Product Quality, Brusselsesteenweg 370, 9090 Melle, Belgium. L.Herman@clo.fgov.be
D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D004272:DNA, Mitochondrial / Q000032:analysis; D004372:Ducks / Q000235:genetics*; D004531:Eggs; D046094:Galliformes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D013045:Species Specificity
D004272:DNA, Mitochondrial; D000251:Adenosine Triphosphatases