Donor and isolation variables associated with human islet monocyte chemoattractant protein-1 release.
Human islets have chemotactic activity toward macrophages mediated by the secretion of monocyte chemoattractant protein-1 (CCL2/MCP-1) that negatively affect clinical outcome in islet after kidney recipients. The aim of the present work was to identify the donor features and the variables involved in the procedures of islet isolation associated with islet CCL2/MCP-1 release in vitro. We used a retrospective approach studying the outcome in 170 islet isolations. The univariate analysis demonstrated that CCL2/MCP-1 release was significantly associated with the surgical team in charge for organ harvesting, the proteins for dilution solution, the type of gradient, the type of enzyme, and the donor noradrenalin treatment. The multivariate analysis confirmed that the surgical team (P = 0.001) and the enzyme (P = 0.001) were independently associated with in vitro CCL2/MCP-1 islet release (r(2) = 17%). Strategies aimed to optimize the procedures of organ harvesting and islet isolation may reduce the pro-inflammatory properties of the preparation and therefore may improve islet engraftment.
Melzi
Raffaella
R
Medicine Department, San Raffaele Scientific Institute, Milan, Italy.
Piemonti
Lorenzo
L
Nano
Rita
R
Clissi
Barbara
B
Calori
Giliola
G
Antonioli
Barbara
B
Marzorati
Simona
S
Perseghin
Gianluca
G
Di Carlo
Valerio
V
Bertuzzi
Federico
F
D018932:Chemokine CCL2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000009:adverse effects / Q000502:physiology*; D008297:Male; D014019:Tissue Donors / Q000706:statistics & numerical data*; D020858:Tissue and Organ Harvesting / Q000379:methods
C111835:CCL2 protein, human; D018932:Chemokine CCL2