Development of a chinchilla model to allow direct, continuous, biophotonic imaging of bioluminescent nontypeable Haemophilus influenzae during experimental otitis media.
We transformed a nontypeable Haemophilus influenzae clinical isolate with a plasmid containing the luxCDABE operon driven by the H. influenzae outer membrane protein P2 promoter. Herein, we demonstrate the ability to detect bioluminescence and to monitor infection within the nasopharynges, eustachian tubes, and middle ears of chinchillas after intranasal and transbullar challenges.
Novotny
Laura A
LA
Center for Microbial Pathogenesis, Columbus Children's Research Institute, The Ohio State University College of Medicine and Public Health, Rm. W591, 700 Children's Dr., Columbus, OH 43205-2696, USA.
Mason
Kevin M
KM
Bakaletz
Lauren O
LO
D000818:Animals; D002682:Chinchilla; D004195:Disease Models, Animal; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D008163:Luminescent Measurements; D010033:Otitis Media / Q000382:microbiology*