Cell enrichment and immunochemical staining.
Cell populations can be enriched using a number of physicochemical methods based on characteristics, such as density, size, and volume. Centrifugation using density gradients is typically applied to isolate populations with similar characteristics. Cells obtained using these methods can be further authenticated using specific antibodies that react to markers on the cell surface, allowing the identification of cell subtypes.
Lara
Oscar R
OR
Department of Chemical Engineering, The Ohio State University, Columbus, OH, USA.
Chalmers
Jeffrey J
JJ
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000954:Antigens, Surface / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*
D000906:Antibodies; D000954:Antigens, Surface