17beta-estradiol regulates the numbers, endocytosis, stimulative capacity and IL-10 secretion of mouse spleen dendritic cells.
The 17beta-estradiol (17beta-E2) is a steroid sex hormone that has a profound influence on the immune cells in inducing the apoptosis of both T and B lymphocytes. In this study, mouse spleen dendritic cells (SDCs) were treated with 17beta-E2 and the numbers, endocytosis, stimulative capacity and cytokine production of SDCs were analysed. The results showed that 17beta-E2 reduced the proliferation and stimulative capacity of SDCs and increased the endocytosis of SDCs in a dose-dependent pattern. 17beta-E2 up-regulated IL-10 mRNA level in SDCs in a dose-dependent manner except for the 24 h time point. These data suggest that 17beta-E2 may regulate the physiological and pathological immune response by reducing the number and stimulation of SDCs, increasing their endocytosis and IL-10 mRNA expression at the same time.
Yang
Linsong
L
Immunology and Reproductive Biology Lab, Medical School, Nanjing University, 22 Hankou Road, Nanjing 210093, PR China.
Liang
Jun
J
Yao
Genhong
G
Chen
Peifu
P
Hou
Yayi
Y
D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D016753:Interleukin-10 / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology*; D013997:Time Factors; D015854:Up-Regulation
D016753:Interleukin-10; D004958:Estradiol