Transgenic expression in tobacco of a poly-proctolin construct leading to production of the bioactive peptide.
Short peptides can be expressed in plants using synthetic genes encoding multiple copies of the peptide spaced by dibasic endoproteolytic cleavage sites. A synthetic gene encoding an array of repeated copies of proctolin, a very well characterized insect myotropic peptide, spaced by Arg residues, was synthesized and expressed in tobacco plants. The successful production of bioactive proctolin from the precursor in transgenic plants was demonstrated by immunoblot, HPLC, mass spectrometry and a bioassay based on the contraction of isolated cockroach hindgut. These results suggest that in planta, as in animals and yeasts, endopeptidases of the serine proteases family may be involved in precursor processing.
Rao
Rosa
R
Dipartimento di Scienze del Suolo, della Pianta e dell'Ambiente, Università degli Studi di Napoli Federico II, Portici, Italy. rao@unina.it
Breuer
Michael
M
Tortiglione
Claudia
C
Malva
Carla
C
Baggerman
Gilon
G
Corrado
Giandomenico
G
De Loof
Arnold
A
Pennacchio
Francesco
F
D000595:Amino Acid Sequence; D000818:Animals; D003058:Cockroaches; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology*; D009479:Neuropeptides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D009842:Oligopeptides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D009479:Neuropeptides; D009842:Oligopeptides; D011994:Recombinant Proteins; C011536:proctolin