[The status of chymeric cells in human to pig spleen transplantation model].
OBJECTIVE
To establish a human to pig xenotransplantation GVHD model to simulate the human anti pig grafts immunoreaction in vivo.


METHODS
The Chinese Neijiang pigs were immunosuppressed by CTX followed by the transplant of human spleen slices into pig's peritoneal cavity. We tested the routine items of the pigs' peripheral blood regularly, monitored the chimeric level by FCM, and examined the cytotoxicities of both human anti pig and pig anti human by CML.


RESULTS
The pigs all survived during the experimental period. The PBWBC decreased by 85% after 4 days of giving CTX. The chimeric peaks appeared on day 2 and day 14 after transplant. The cytotoxicity of human anti pig was always less than that of pig anti human, although it increased significantly after 2 weeks of transplant.


CONCLUSIONS
There might be a dynamic changeable balance of HVG, microchimerism, and GVH due to the reaction between the chimeric human cells and the pig cells in the human to pig spleen xenotransplantation model.
Zhang
Haitao
H
Lab of Transplant Immunology and Transplant Engineering, West China Hospital, SiChuan University, Chengdu, China.
Li
Youping
Y
Wang
Kunjie
K
Li
Shengfu
S
Cheng
Jingqiu
J
D000818:Animals; D003520:Cyclophosphamide / Q000627:therapeutic use; D006086:Graft vs Host Disease; D006789:Host vs Graft Reaction / Q000276:immunology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008297:Male; D010529:Peritoneal Cavity / Q000601:surgery; D013154:Spleen / Q000166:cytology / Q000637:transplantation*; D013552:Swine; D018183:Transplantation Chimera / Q000502:physiology*; D014183:Transplantation, Heterologous / Q000276:immunology*
D007166:Immunosuppressive Agents; D003520:Cyclophosphamide