[Anti-virus effect of hepatocyte stimulating substance (HSS) on duck hepatitis B virus in vivo].
We used one day old Beijing ducklings infected by duck hepatitis B virus as experimental model to observe anti-virus effect of HSS. Beijing ducklings hatched within one day were injected intravenously with duck hepatitis B virus (DHBV). Positive DHBV-DNA in ducklings' sera were detected 7 days later. Serum samples were obtained from all ducklings before, during and after drug treatment at different times and stored at -70 degrees C. The collected samples were detected DHBV-DNA by dot-blot hybridization test at the same time. The therapeutic effects were evaluated by OD value of dot-blot and by the inhibitory rate of DHBV-DNA. The results showed that HSS (with 50 mg/kg/day and 200 mg/kg/day) were effective in inhibition of DHBV-DNA during and after drug treatment, especially in the HSS group of large dosage. The inhibitory effect appeared early and high dynamic. The inhibitory rate of DHBV-DNA during and after drug treatment were higher than the NS group (P < 0.05). No inhibitory effect was observed in NS group. The hepatic biopsy showed HSS groups had milder damage than NS group, indicating that the HSS could inhibit the replication of DHBV-DNA in Beijing ducklings.
Chen
G
G
The 302 Hospital of PLA, Beijing 100039.
Chen
J
J
Liu
D
D
Zhang
X
X
Zhu
C
C
Li
Y
Y
Wang
L
L
Yang
Y
Y
Cai
G
G
Han
F
F
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000188:drug therapy* / Q000662:veterinary* / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy* / Q000821:virology; D015850:Interleukin-6 / Q000008:administration & dosage*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004279:DNA, Viral; D015850:Interleukin-6