Effects of crude oil on phospholipid fatty acid compositions of marine hydrocarbon degraders: estimation of the bacterial membrane fluidity.
In this study, we investigated, in vitro, the effects of petroleum hydrocarbons on the phospholipid ester-linked fatty acid composition of Corynebacterium sp. Strain 8. The usual ratio of monounsaturated fatty acids E/Z (or trans/cis) was calculated. This ratio led to unexpected results because we found similar values for growths on either a hydrophobic substrate (crude oil) or a soluble carbon source (rich medium). The use of such an indicator seemed limited for monitoring an environmental stress, so we proposed an index based on the homeoviscous adaptation theory. A membrane viscosity index was defined and applied to Corynebacterium sp. Strain 8 (in vitro growth) and to a sedimentary community (in situ experiment). The results allowed us to estimate the membrane fluidity of both an isolated strain and a bacterial community in accordance with the medium hydrophobicity.
Mazzella
N
N
Laboratoire de Chimie Analytique de l'Environnement, Université d'Aix Marseille, UMR 6171, IFR PMSE 112, Europôle de l'Arbois, BP 80, 13545 Aix-en-Provence Cedex 4, France.
Syakti
A D
AD
Molinet
J
J
Gilewicz
M
M
Doumenq
P
P
Artaud
J
J
Bertrand
J-C
JC
D001673:Biodegradation, Environmental; D003352:Corynebacterium / Q000187:drug effects* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008560:Membrane Fluidity; D010578:Petroleum / Q000633:toxicity*; D010743:Phospholipids / Q000378:metabolism*
D010578:Petroleum; D010743:Phospholipids