Effects of dietary supplementation with polyunsaturated fatty acids and antioxidants on biochemical characteristics of boar semen.
The aim of this study was to investigate the effect of providing a supplement containing polyunsaturated fatty acids and antioxidants (PROSPERM) on the biochemical characteristics of boar semen. Two sexually mature boars were fed a standard diet with PROSPERM (250 g daily) for a 24-week period. Ejaculates collected prior to supplementation were used as the control. Semen quality and biochemical parameters were analyzed. The dietary supplementation enhanced sperm characteristics, including the percentage of spermatozoa with intact plasma membrane and osmotic resistance of the acrosomal membrane. Higher production of malondialdehyde was concurrent with increased activity of superoxide dismutase in the seminal plasma and spermatozoa after 8 weeks of supplementation. These changes were accompanied by a high content of total protein and low-molecular antioxidants of the seminal plasma. It was observed that PROSPERM supplementation enhanced the survivability of boar spermatozoa during storage in a standard semen extender supplemented with lipoprotein fractions, isolated from hen egg yolk or ostrich egg yolk, at 5 degrees C and 16 degrees C. These results indicate that PROSPERM supplementation of boars had a beneficial effect on the biological characteristics of the spermatozoa, which could be useful for semen preservation at different temperatures.
Strzezek
Jerzy
J
Department of Animal Biochemistry and Biotechnology, Warmia and Mazury University in Olsztyn, Faculty of Animal Bioengineering, Oczapowskiego 5, 10-718 Olsztyn, Poland. strzezek@uwm.edu.pl
Fraser
Leyland
L
Kuklińska
Magda
M
Dziekońska
Anna
A
Lecewicz
Marek
M
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000032:analysis; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D019587:Dietary Supplements; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D005978:Glutathione / Q000032:analysis; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012661:Semen / Q000737:chemistry* / Q000201:enzymology; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine
D000975:Antioxidants; D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D011506:Proteins; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid