Cytogenetic changes and loss of heterozygosity in atypical adenomatous hyperplasia, in carcinoma of the prostate and in non-neoplastic prostate tissue using comparative genomic hybridization and multiplex-PCR.
High grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia (AAH) are discussed to be precursors of prostate cancer (PC). Unlike high grade PIN the relation between AAH and PC is however unclear. In the present study we analyzed AAH, accompanying prostate carcinomas and carcinomas of the transitional zone after microdissection using comparative genomic hybridization (CGH) and multipelx-PCR with 10 microsatellite polymorphic markers. In every case non-neoplastic prostatic tissue was investigated for the same allelic imbalances. Two AAH showed allelic imbalances in multiplex-PCR. These imbalances did not correlate with the corresponding tumours and furthermore were different to the LOH found in the investigated prostate tumours of the transitional zone. One AAH showed loss on chromosome 22q. We found allelic imbalances in over 50% of non-neoplastic tissue adjacent to prostatic carcinoma. Our findings support the idea that AAH does not seem to be linked closely to PC and should not be considered as an obligate premalignant lesion.
Bettendorf
Olaf
O
Institute of Pathology, University of Münster, D-48149 Münster, Germany. olaf.bettendorf@ukmuenster.de
Schmidt
Hartmut
H
Eltze
Elke
E
Gockel
Iris
I
Semjonow
Axel
A
Bürger
Horst
H
Böcker
Werner
W
Brandt
Burkhard
B
D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D002277:Carcinoma / Q000235:genetics* / Q000276:immunology; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D020732:Cytogenetic Analysis; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000032:analysis / Q000378:metabolism; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000166:cytology / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000276:immunology
D014408:Biomarkers, Tumor; D007633:Keratins