title: Simultaneous determination of mercury and arsenic species in natural freshwater by liquid chromatography with on-line UV irradiation, generation of hydrides and cold vapor and tandem atomic fluorescence detection.
abstract: An approach based on the coupling HPLC-UV-CV/HG-mAFS-AFS has been developed for the simultaneous determination of mercury and arsenic species. A home modified AFS detector has been used for the analysis of mercury coupled in series with another similar detector for arsenic determination. The determined species were Hg2+, methylmercury (MeHg+), As(III), As(V) and monomethylarsinate (MMA). A critical aspect is the chromatographic separation, which was carefully optimized for the separation of all the species. The detection limits for these species are 11, 8, 17, 17 and 3 ng ml(-1), respectively. Linear curves for MMA were obtained between 10 and 200 ng ml(-1). The linear dynamic range for all the other species was comprised between the detection limit and 500 ng ml(-1). The influence of cations and anions at the concentration usually present in natural freshwater was studied. The procedure was validated by application to spiked natural freshwater samples from the south-west Spain, and it can be considered for routine analysis of polluted sites.
authors.affiliation: Dpto. Química y Ciencias de los Materiales, Facultad de Ciencias Experimentales, Universidad de Huelva, Campus de El Carmen, 21007 Huelva, Spain. ariza@uhu.es
authors.forename: José Luis
authors.initials: JL
authors.lastname: Gómez-Ariza
authors.forename: F
authors.initials: F
authors.lastname: Lorenzo
authors.forename: T
authors.initials: T
authors.lastname: García-Barrera
mesh_terms: D001151:Arsenic / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry*; D008628:Mercury / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D008628:Mercury; D001151:Arsenic