Endogenous prostacyclin increases neuronal nitric oxide release in mesenteric artery from spontaneously hypertensive rats.
The aim of this study was to analyse the possible influence of endogenous prostacyclin on neuronal nitric oxide (NO) release induced by electrical field stimulation in mesenteric arteries from spontaneously hypertensive rats (SHR). Preincubation with the prostacyclin synthesis inhibitor tranylcypromine decreased NO release induced by electrical field stimulation, which was reversed by exogenous prostacyclin. Preincubation with tranylcypromine increased basal and electrical field stimulation-induced [3H]noradrenaline release. The nitric oxide synthase inhibitor Nomega-nitro-L-arginine methyl esther (L-NAME) increased the vasoconstrictor response induced by electrical field stimulation. In the presence of tranylcypromine, L-NAME did not modify the vasoconstrictor response induced by electrical field stimulation. In the presence of tranylcypromine and prostacyclin, LNAME increased the vasoconstrictor response to electrical field stimulation. These results indicate that endogenous prostacyclin positively modulates the neuronal NO release induced by electrical field stimulation and that this neuronal NO participates in the regulation of the vasomotor response.
Ferrer
Mercedes
M
Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma, C/ Arzobispo Morcillo, 4, 28029- Madrid, Spain.
Salaices
Mercedes
M
Balfagón
Gloria
G
D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism* / Q000502:physiology; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D044062:Prostaglandins I / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D013779:Tetrodotoxin / Q000494:pharmacology; D014191:Tranylcypromine / Q000494:pharmacology
D000779:Anesthetics, Local; D008996:Monoamine Oxidase Inhibitors; D009419:Nerve Tissue Proteins; D044062:Prostaglandins I; D009569:Nitric Oxide; D014191:Tranylcypromine; D013779:Tetrodotoxin; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat