title: ABA associated biochemical changes during somatic embryo development in Camellia sinensis (L.) O. Kuntze.
abstract: The effect of ABA on reserve accumulation in maturing somatic embryos of tea was compared with and without ABA treatment. Changes in the levels of starch, total soluble sugars (TSS), proteins, and phenols were studied in the somatic embryos at different stages of development (globular, heart, torpedo and germinating embryos) in order to investigate whether ABA could trigger accumulation of storage reserves and thereby overcome the problem of poor germination. After ABA treatment (5.0 mg l(-1)) for 14 days, the starch and protein contents that were negligible in the untreated embryos increased by several fold with a simultaneous increase in TSS. When ABA treatment occurred at the heart stage, the germination of the embryos also improved, relative to untreated controls, after ABA treatment. ABA treatment prior to or after heart stage did not improve somatic embryo germination.
authors.affiliation: Division of Biotechnology, Institute of Himalayan Bioresource Technology, Palampur-176 061, H.P., India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Sharma
authors.forename: Subedar
authors.initials: S
authors.lastname: Pandey
authors.forename: Amita
authors.initials: A
authors.lastname: Bhattacharya
authors.forename: P K
authors.initials: PK
authors.lastname: Nagar
authors.forename: Paramvir Singh
authors.initials: PS
authors.lastname: Ahuja
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology*; D028241:Camellia sinensis / Q000187:drug effects* / Q000196:embryology*; D018525:Germination / Q000187:drug effects; D010636:Phenols / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D012639:Seeds / Q000187:drug effects* / Q000254:growth & development*; D013213:Starch / Q000378:metabolism
chemical_list: D010636:Phenols; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid; D013213:Starch