title: Conjugation of antibodies to fluorescein or rhodamine.
abstract: The isothiocyanate derivative of the fluorophore, fluorescein or rhodamine, is coupled to the amino groups of IgG antibody in a one-step procedure and excess label is removed by gel filtration.
authors.affiliation: School of Biology and Biochemistry, The Queen's University, Belfast, Northern Ireland, UK.
authors.forename: G Brian
authors.initials: GB
authors.lastname: Wisdom
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D019793:Fluorescein / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D012235:Rhodamines; D019793:Fluorescein