title: Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice.
abstract: SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID(5.0) of the live virus 10(3.6) to 10(4.6) times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed.
authors.affiliation: Key Laboratory of Medical Molecular Virology/Ministry of Education, Ministry of Public Health, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Qu
authors.forename: Bojian
authors.initials: B
authors.lastname: Zheng
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.forename: Yi
authors.initials: Y
authors.lastname: Guan
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Yuan
authors.forename: Nan-Shan
authors.initials: NS
authors.lastname: Zhong
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Lu
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xie
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Wen
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood*; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D008168:Lung / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014709:Vero Cells; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines