[Studies on hydrolysis of anti-HRV IgY and its oral passive immunity effect to human rotavirus].
In this paper, hens of fine breed were immunized with human rotavirus (HRV), and the anti-HRV IgY was isolated and purified from their eggs collected daily. In addition, the resistance of anti-HRV IgY to hydrolysis of gastric juice and proteases in human digestive tract, the safety of IgY and the effectiveness of IgY in clinical use were observed as well. The results showed that anti-HRV IgY has a fairly good resistance to gastroinstestinal proteases. The safety of using anti-HRV IgY was affirmed by oral administration to mice of a solution of IgY. In clinical test the IgY has been proved to be anti-HRV and, therefore, effective against infections of infant diarrhea induced by HRV.
Long
Z
Z
Sino-German Joint Reseach Institute, Nanchang 330047.
Zhong
Q
Q
Zhu
Y
Y
Xiong
Y
Y
Chen
H
H
Yang
N
N
Yang
R
R
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D002645:Chickens; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D051379:Mice; D055550:Protein Stability; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology / Q000628:therapy*
D000914:Antibodies, Viral; C016512:IgY; D007136:Immunoglobulins