title: Point mutation of 5' noncoding region of BCL-6 gene in primary gastric lymphomas.
abstract: OBJECTIVE
  To investigate the mutations of the 5' noncoding region of BCL-6 gene in Chinese patients with primary gastric lymphomas.
  
  
  METHODS
  PCR and direct DNA sequencing were used to identify BCL-6 gene mutations in the 5' noncoding region in 29 cases of gastric diffuse large B-cell lymphoma (DLBCL) and 18 cases of gastric mucosa-associated lymphoid tissue (MALT) lymphoma as well as 10 cases of reactive hyperplasia of lymph node (LRH).
  
  
  RESULTS
  Six of 29 gastric DLBCLs (20.7%), 4 of 18 gastric MALT lymphomas (22.2%) and 1 of 10 LRHs(10%) were found to have mutations. All mutations were single-base substitutions and the frequency of single-base changes was 0.20 x 10(-2) -1.02 x 10(-2) per bp.
  
  
  CONCLUSIONS
  Point mutations in the 5' noncoding region of BCL-6 gene are found in Chinese patients with primary gastric DLBCLs and MALT lymphomas, suggesting that they may, in some extent, participate in the pathogenesis of primary gastric DLBCLs and MALT lymphomas.
authors.affiliation: Laboratory of Molecular Pathology, Cancer Hospital of Fudan University, Shanghai 200032, China.
authors.forename: Da-Liu
authors.initials: DL
authors.lastname: Min
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yang
authors.forename: Hong-Fen
authors.initials: HF
authors.lastname: Lu
authors.forename: Tai-Ming
authors.initials: TM
authors.lastname: Zhang
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Zhen
authors.forename: Pei-Zheng
authors.initials: PZ
authors.lastname: Cao
authors.forename: Da-Ren
authors.initials: DR
authors.lastname: Shi
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008223:Lymphoma / Q000235:genetics*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051560:Proto-Oncogene Proteins c-bcl-6; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors