title: Improvement of nisin production in pH feed-back controlled, fed-batch culture by Lactococcus lactis subsp. lactis.
abstract: Nisin production by Lactococcus lactis subsp. lactis in fed-batch culture was doubled by using a pH feed-back controlled method. Sucrose concentration was controlled at 10 g l(-1) giving 5010 IU nisin ml(-1) compared to 2660 IU nisin ml(-1) in batch culture.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, P.O. Box 353, Beijing, 100080, P.R. China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Lv
authors.forename: Wei
authors.initials: W
authors.lastname: Cong
authors.forename: Zhaoling
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D005246:Feedback / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D009561:Nisin / Q000096:biosynthesis*; D013395:Sucrose / Q000378:metabolism*
chemical_list: D009561:Nisin; D013395:Sucrose