Interaction with coxsackievirus and adenovirus receptor, but not with decay-accelerating factor (DAF), induces A-particle formation in a DAF-binding coxsackievirus B3 isolate.
Although many coxsackie B viruses interact with decay accelerating factor (DAF), attachment to DAF by itself is not sufficient to initiate infection. We examined the early events in infection that follow virus interaction with DAF, and with the coxsackievirus and adenovirus receptor (CAR). Interaction with soluble CAR in a cell-free system, or with CAR on the surfaces of transfected cells, induced the formation of A particles; interaction with soluble or cell surface DAF did not. The results suggest that CAR, but not DAF, is capable of initiating the conformational changes in the viral capsid that lead to release of viral nucleic acid.
Milstone
Aaron M
AM
Division of Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.
Petrella
JenniElizabeth
J
Sanchez
Melissa D
MD
Mahmud
Mariam
M
Whitbeck
J Charles
JC
Bergelson
Jeffrey M
JM
D000818:Animals; D018958:CD55 Antigens / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D002213:Capsid / Q000737:chemistry; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D029822:Enterovirus B, Human / Q000378:metabolism / Q000472:pathogenicity*; D006367:HeLa Cells; D006801:Humans; D011487:Protein Conformation; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014771:Virion / Q000378:metabolism*
D018958:CD55 Antigens; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus