[The multi-locus VNTR-analysis in studies of the population structure of Yersinia pestis in natural foci].
A collection of Yersinia pestis strains was investigated by the multi-locus VNTR analysis. All 9 used locuses were diverse, although they differed between themselves by the quantity of genotypes displaying 4 to 13 variations in the sample. The diversity index (DI) ranged from 0.18 (ms21) to 0.86 (ms46); 8 locuses had DI > 0.5. The statistical processing showed 55 individual genotypes in a group of 81 examined strains, which denoted a high discriminative potentiality of the typing system (DP = 0.98). On the basis of the cluster analysis, the genotypes were shared between 11 main groups. The strains belonging to one genotype group were found to originate, as a rule, from one natural focus. The suggested scheme of typing and of creating the databases of genotypes of plaque agent can be used to establish, with a high probability degree, the source of strains.
Suchkov
I Iu
IIu
Vodop'ianov
A S
AS
Vodop'ianov
S O
SO
Shishiianu
M V
MV
Mishan'kin
B N
BN
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010930:Plague / Q000382:microbiology / Q000517:prevention & control; D016133:Polymerase Chain Reaction; D012426:Russia; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020080:Tandem Repeat Sequences; D014744:Vietnam; D015010:Yersinia pestis / Q000145:classification / Q000235:genetics*