Optimisation of gradient HPLC analysis of phenolic compounds and flavonoids in beer using a coularray detector.
A method was developed for simultaneous analysis of natural antioxidants in beer using multichannel electrochemical detection with a CoulArray detector, which enables selective and sensitive antioxidant detection in gradient HPLC and facilitates the identification of analytes based on the ratios of signals recorded at different potentials applied to the detection cells arranged in series. The separation conditions were optimised for 27 phenolic compounds including derivatives of benzoic and cinnamic acids, flavones, and a few related glycosides identified in beer samples. Separation selectivities of 11 columns with different stationary phase chemistries were compared, and the pH and gradient programs were optimised for the individual columns to provide best resolution and high number of resolved peaks, using the window-diagram approach. The effects of pH on the sensitivity of electrochemical coulometric detection were considered in the optimisation approach. The optimised conditions were applied to the analysis of real beer samples.
Rehová
Lucie
L
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Department of Analytical Chemistry, CZ-162 06 Prague 6, Czech Republic.
Skeríková
Veronika
V
Jandera
Pavel
P
D000975:Antioxidants / Q000737:chemistry; D001515:Beer / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols