title: Complete comparative genomic analysis of two field isolates of Mamestra configurata nucleopolyhedrovirus-A.
abstract: A second genotype of Mamestra configurata nucleopolyhedrovirus-A (MacoNPV-A), variant 90/4 (v90/4), was identified due to its altered restriction endonuclease profile and reduced virulence for the host insect, M. configurata, relative to the archetypal genotype, MacoNPV-A variant 90/2 (v90/2). To investigate the genetic differences between these two variants, the genome of v90/4 was sequenced completely. The MacoNPV-A v90/4 genome is 153 656 bp in size, 1404 bp smaller than the v90/2 genome. Sequence alignment showed that there was 99.5 % nucleotide sequence identity between the genomes of v90/4 and v90/2. However, the v90/4 genome has 521 point mutations and numerous deletions and insertions when compared to the genome of v90/2. Gene content and organization in the genome of v90/4 is identical to that in v90/2, except for an additional bro gene that is found in the v90/2 genome. The region between hr1 and orf31 shows the greatest divergence between the two genomes. This region contains three bro genes, which are among the most variable baculovirus genes. These results, together with other published data, suggest that bro genes may influence baculovirus genome diversity and may be involved in recombination between baculovirus genomes. Many ambiguous residues found in the v90/4 sequence also reveal the presence of 214 sequence polymorphisms. Sequence analysis of cloned HindIII fragments of the original MacoNPV field isolate that the 90/4 variant was derived from indicates that v90/4 is an authentic variant and may represent approximately 25 % of the genotypes in the field isolate. These results provide evidence of extensive sequence variation among the individual genomes comprising a natural baculovirus outbreak in a continuous host population.
authors.affiliation: Pacific Agri-Food Research Centre, AAFC, Summerland, BC, Canada.
authors.forename: Lulin
authors.initials: L
authors.lastname: Li
authors.affiliation: Saskatoon Research Centre, AAFC-Saskatoon, SK, Canada.
authors.forename: Qianjun
authors.initials: Q
authors.lastname: Li
authors.affiliation: Pacific Agri-Food Research Centre, AAFC, Summerland, BC, Canada.
authors.forename: Leslie G
authors.initials: LG
authors.lastname: Willis
authors.affiliation: Saskatoon Research Centre, AAFC-Saskatoon, SK, Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Erlandson
authors.affiliation: Pacific Agri-Food Research Centre, AAFC, Summerland, BC, Canada.
authors.forename: David A
authors.initials: DA
authors.lastname: Theilmann
authors.affiliation: Southern Crop Protection and Food Research Centre, AAFC, London, ON, Canada.
authors.forename: Cam
authors.initials: C
authors.lastname: Donly
mesh_terms: D000818:Animals; D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D007814:Larva / Q000821:virology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000302:isolation & purification; D011576:Psychodidae / Q000821:virology*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity