title: [Establishment of two-dimensional electrophoresis of uterine leiomyoma for the proteome analysis].
abstract: To establish and optimize the two-dimensional electrophoresis (2-DE) of uterine leiomyoma for the proteome analysis.
  
  
  
  Run immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.
  
  
  
  The 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.
  
  
  
  With optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.
authors.affiliation: Women's Hospital, College of Medicine, Zhejiang University, Hangzhou, 310006, China.
authors.forename: Xue-qiong
authors.initials: XQ
authors.lastname: Zhu
authors.forename: Yi-fu
authors.initials: YF
authors.lastname: Shi
authors.forename: Huai-zeng
authors.initials: HZ
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000737:chemistry*; D009215:Myometrium / Q000737:chemistry; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D014594:Uterine Neoplasms / Q000737:chemistry*
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome