The Ala-9Val polymorphism in the mitochondrial targeting sequence (MTS) of the manganese superoxide dismutase gene is not associated with juvenile-onset asthma.
BACKGROUND
We aimed to investigate the possible association between Mn-SOD polymorphism in the mitochondrial targeting sequence and asthma.


METHODS
Alanine or valine polymorphism in the signal peptide of Mn-SOD gene was evaluated using a primer pair to amplify a 107-bp fragment followed by digestion with NgoM IV.


RESULTS
No significant difference in genotype frequencies was found between patients and controls.


CONCLUSIONS
These results suggest no major modifying role for the Mn-SOD gene polymorphism in patients with asthma.
Gurel
Ahmet
A
Department of Biochemistry, Zonguldak Karaelmas University Medical Faculty, Zonguldak, Turkey. dragurel@yahoo.com
Tomac
Nazan
N
Yilmaz
H Ramazan
HR
Tekedereli
Ibrahim
I
Akyol
Omer
O
Armutcu
Ferah
F
Yuce
Huseyin
H
Akin
Haluk
H
Ozcelik
Nurten
N
Elyas
Halit
H
D000293:Adolescent; D017668:Age of Onset; D000409:Alanine / Q000235:genetics*; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005838:Genotype; D006801:Humans; D007223:Infant; D008345:Manganese; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014633:Valine / Q000235:genetics*
D010455:Peptides; D008345:Manganese; D013482:Superoxide Dismutase; D014633:Valine; D000409:Alanine