Adenoviral transduction of dendritic cells.
Recombinant adenoviral (rAd) vectors are highly suitable for efficient genetic modification of dendritic cells (DC). In certain cases, the high immunogenicity of rAd may be a disadvantage. This chapter describes the essential aspects of optimal rAd-mediated gene transfer into DC, discusses the consequences of the immunogenicity of rAd vectors, and provides suggestions to minimize these complications.
Offringa
Rienk
R
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Kwappenberg
Kitty
K
Rabelink
Martijn
M
Rea
Delphine
D
Hoeben
Rob
R
D000256:Adenoviridae / Q000235:genetics*; D002454:Cell Differentiation; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D017186:Titrimetry; D014161:Transduction, Genetic / Q000379:methods*
D018950:Lipopolysaccharide Receptors