title: Gene expression in Plasmodium berghei ookinetes and early oocysts in a co-culture system with mosquito cells.
abstract: Using an in vitro development system for Plasmodium berghei sporogonic stages and microarray technology we examined parasite gene expression during ookinete invasion of Aedes cells and the ensuing oocyst development. A number of genes were found to be differentially expressed. The most prominent class of up-regulated elements corresponded to products involved in protein synthesis and metabolism. Furthermore, several previously studied genes with a known in vivo developmental profile matched published data. A large number of genes with a hitherto unknown function during the life cycle stages studied also show a differential pattern of expression, indicating the involvement of their products in control and execution of active developmental processes.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Vassilika Vouton, Box 1527, 711 10 Heraklion, Crete, Greece.
authors.forename: John
authors.initials: J
authors.lastname: Vontas
authors.forename: Inga
authors.initials: I
authors.lastname: Siden-Kiamos
authors.forename: Giorgos
authors.initials: G
authors.lastname: Papagiannakis
authors.forename: Marianna
authors.initials: M
authors.lastname: Karras
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Waters
authors.forename: Christos
authors.initials: C
authors.lastname: Louis
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016000:Cluster Analysis; D018920:Coculture Techniques; D009033:Culicidae / Q000166:cytology / Q000469:parasitology*; D004734:Energy Metabolism; D004847:Epithelial Cells / Q000469:parasitology; D015870:Gene Expression; D020869:Gene Expression Profiling; D017125:Genes, Protozoan; D008018:Life Cycle Stages; D020411:Oligonucleotide Array Sequence Analysis; D010962:Plasmodium berghei / Q000235:genetics* / Q000254:growth & development; D014176:Protein Biosynthesis / Q000235:genetics