Increased risk of intestinal type of gastric adenocarcinoma in Japanese women associated with long forms of CCTTT pentanucleotide repeat in the inducible nitric oxide synthase promoter.
Tandem repeat number polymorphism of a CCTTT pentanucleotide in the promoter region of the inducible nitric oxide synthase gene (iNOS) and a polymorphism of the interleukin-1beta (IL-1B) promoter at position -31 were analyzed in DNA samples from 181 Japanese control subjects and 158 gastric cancer patients, including 96 intestinal type and 62 diffuse type. An association between the intestinal type of gastric adenocarcinoma and higher promoter activity of the iNOS gene was found in women, especially those having higher promoter activity of the IL-1B gene and without a history of smoking. Our results imply that chronic inflammation caused by excess nitric oxide generated by iNOS contributes to Helicobacter pylori-induced gastric cancer.
Tatemichi
Masayuki
M
International Agency for Research on Cancer, 150 Cours Albert Thomas, F 69372 Lyon Cedex 08, France.
Sawa
Tomohiro
T
Gilibert
Isabelle
I
Tazawa
Hiroshi
H
Katoh
Takahiko
T
Ohshima
Hiroshi
H
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D005260:Female; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics; D016480:Helicobacter pylori; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D007564:Japan; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D012737:Sex Factors; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm; D007375:Interleukin-1; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II