title: Transcriptional control by A-factor of two trypsin genes in Streptomyces griseus.
abstract: AdpA is the key transcriptional activator for a number of genes of various functions in the A-factor regulatory cascade in Streptomyces griseus, forming an AdpA regulon. Trypsin-like activity was detected at a late stage of growth in the wild-type strain but not in an A-factor-deficient mutant. Consistent with these observations, two trypsin genes, sprT and sprU, in S. griseus were found to be members of the AdpA regulon; AdpA activated the transcription of both genes by binding to the operators located at about -50 nucleotide positions with respect to the transcriptional start point. The transcription of sprT and sprU, induced by AdpA, was most active at the onset of sporulation. Most trypsin activity exerted by S. griseus was attributed to SprT, because trypsin activity in an sprT-disrupted mutant was greatly reduced but that in an sprU-disrupted mutant was only slightly reduced. This was consistent with the observation that the amount of the sprT mRNA was much greater than that of the sprU transcript. Disruption of both sprT and sprU (mutant DeltasprTU) reduced trypsin activity to almost zero, indicating that no trypsin genes other than these two were present in S. griseus. Even the double mutant DeltasprTU grew normally and developed aerial hyphae and spores over the same time course as the wild-type strain.
authors.affiliation: Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Jun-Ya
authors.initials: JY
authors.lastname: Kato
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Chi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Soon-Kwang
authors.initials: SK
authors.lastname: Hong
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000502:physiology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D013305:Streptomyces griseus / Q000235:genetics*; D015534:Trans-Activators / Q000378:metabolism; D014158:Transcription, Genetic; D014357:Trypsin / Q000235:genetics*
chemical_list: C401645:AdpA protein, Streptomyces griseus; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C025035:A-factor (Streptomyces); D014357:Trypsin; D015107:4-Butyrolactone