title: Compensatory response of IL-1 gene knockout mice after pulmonary infection with Klebsiella pneumoniae.
abstract: This study was designed to determine the role of interleukin (IL)-1 in the inflammatory response against experimentally induced pneumonia caused by Klebsiella pneumoniae. The host immune responses of IL-1 gene knockout (IL-1 KO) mice and immunocompetent wild-type (WT) mice were compared after pulmonary infection with K. pneumoniae. There were no significant differences between the survival rates and viable bacterial counts in lungs and blood of IL-1 KO and WT mice after pulmonary infections under different conditions. Histopathological analysis showed a similar inflammatory response in both groups of mice. However, in the early stage of infection, the level of tumour necrosis factor alpha (TNF-alpha) in homogenized lungs of IL-1 KO mice was significantly higher than in WT mice. To determine the role of endogenous TNF-alpha in the recovery of the defence mechanism in IL-1 KO mice, mice were treated with an anti-TNF-alpha mAb before infection with K. pneumoniae. The results revealed a significantly lower survival rate of anti-TNF-alpha mAb-treated IL-1 KO mice than BSA-treated IL-1 KO mice. The data suggest that compensatory production of TNF-alpha in IL-1 KO mice contributes to the host defence against K. pneumoniae infection.
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tanabe
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shibuya
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tateda
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Miyazaki
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Nakane
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Iwakura
authors.affiliation: Department of Microbiology1 and Department of Pathology2, Omori Hospital, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku Tokyo, Japan 3Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan 4Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D007375:Interleukin-1 / Q000235:genetics* / Q000276:immunology*; D007710:Klebsiella Infections / Q000276:immunology*; D007711:Klebsiella pneumoniae / Q000276:immunology* / Q000302:isolation & purification; D008168:Lung / Q000737:chemistry / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018410:Pneumonia, Bacterial / Q000276:immunology* / Q000382:microbiology*; D016019:Survival Analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology*
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha