title: The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum.
abstract: Recently, we described the molecular identification of dendritic cell-specific TrAnsMembrane protein (DC-STAMP), a multimembrane-spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC-STAMP (mDC-STAMP) as well as the characterization of the DC-STAMP protein. The results demonstrate that mDC-STAMP is over 90% homologous to hDC-STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC-STAMP expression is enhanced by interleukin-4 and down-regulated upon DC maturation. Analysis of differently tagged DC-STAMP proteins further demonstrates that hDC-STAMP and mDC-STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC-STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC-STAMP-green fluorescent protein fusion protein. These data imply that DC-STAMP may exert its effect in the ER.
authors.affiliation: NCMLS/187 TIL University Medical Center, Postbox 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Eleveld-Trancikova
authors.forename: Vassilis
authors.initials: V
authors.lastname: Triantis
authors.forename: Veronique
authors.initials: V
authors.lastname: Moulin
authors.forename: Maaike W G
authors.initials: MW
authors.lastname: Looman
authors.forename: Mietske
authors.initials: M
authors.lastname: Wijers
authors.forename: Jack A M
authors.initials: JA
authors.lastname: Fransen
authors.forename: Angelique A C
authors.initials: AA
authors.lastname: Lemckert
authors.forename: Menzo J E
authors.initials: MJ
authors.lastname: Havenga
authors.forename: Carl G
authors.initials: CG
authors.lastname: Figdor
authors.forename: Richard A J
authors.initials: RA
authors.lastname: Janssen
authors.forename: Gosse J
authors.initials: GJ
authors.lastname: Adema
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: C499636:DC-STAMP protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins