The effect of the anti-inflammatory cytokines interleukin-4 and interleukin-10 on lipopolysaccharide-stimulated production of prostaglandin E2 by cultured human decidual cells.
OBJECTIVE
We hypothesized that interleukin-4 (IL-4) and interleukin-10 (IL-10) diminish production of PGE2 by lipopolysaccharide (LPS)-stimulated cultured human decidual cells.


METHODS
Decidual cells from six women undergoing elective cesarean delivery without labor at term were cultured to confluence and incubated with LPS (10 ng/mL) with and without IL-4 and IL-10 (10 ng/mL) and the supernatant assayed for PGE2.


RESULTS
PGE2 concentration in non-treated cells (NT) was 16,693+/-8991 pg/mL and in cells incubated with IL-4 alone was 13,490+/-5729 pg/mL, not statistically different from that of the NT cells. Incubation with LPS increased PGE2 concentration (32,540+/-18,795 pg/mL) compared to NT cells (p=0.02). PGE2 concentration in cells co-incubated with IL-4 and LPS (8975+/-5249 pg/mL) was lower than in the LPS-alone group (p=0.005). PGE2 concentration in cells co-incubated with IL-10 and LPS was 29,644+/-25,085 pg/mL, not different from the LPS-alone group.


CONCLUSIONS
IL-4 reduced LPS-stimulated PGE2 production in decidual cells while IL-10 did not. IL-4 is a potential immunomodulatory agent in decidual inflammation.
Simhan
Hyagriv N
HN
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 300 Halket Street, Pittsburgh, PA 15213, USA. hsimhan@mail.magee.edu
Chura
Justin C
JC
Rauk
Phillip N
PN
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D011247:Pregnancy
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; C482199:lipopolysaccharide, E coli O55-B5; D016753:Interleukin-10; D015847:Interleukin-4; D015232:Dinoprostone