[The genetic variability of the protease-encoding region in HIV-1 A isolates and in CRF03_AB recombinants as observed in the CIS territory].
Protease-encoding nucleotide sequences of 27 HIV-1 variants isolated in Russia and other CIS countries from seropositive intravenous drug-users were analyzed. None of the above persons did ever take antiretroviral drugs. The nucleotide sequences were shown to belong to subtypes A and to be have a high degree of genetic homogeneity (0.00-3.23; mean--1.38 +/- 0.79). No isolates contained any primary mutations of resistance to protease inhibitors. At the same time, above one half of the isolates bore the V771 substitution, which, according to published data, is the secondary mutation of resistance that conditions a higher resistance to Nelfinavir. Moreover, the substitution was associated with 2 synonymous mutations in triplets 31 and 78, which denotes a single origin for all V771 variants.
Sukhanova
A L
AL
Kazennova
E V
EV
Rudinskiĭ
N I
NI
Mikhaĭlovich
V M
VM
Griadunov
D A
DA
Budilov
A V
AV
Bulavkina
M A
MA
Weber
J N
JN
Pokrovskiĭ
V V
VV
Bobkov
A F
AF
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D016333:HIV Protease / Q000235:genetics*; D006679:HIV Seropositivity / Q000453:epidemiology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D016415:Sequence Alignment; D015819:Substance Abuse, Intravenous / Q000453:epidemiology*; D014455:Ukraine / Q000453:epidemiology
D012367:RNA, Viral; D016333:HIV Protease