Proteomic analysis of human natural killer cells: insights on new potential NK immune functions.
Applying high-throughput proteomic analysis of mammalian cells can facilitate the identification of a large number of proteins expressed in the examined samples. Moreover, extensive research efforts are being made to perform large-scale characterization of membrane proteins. Here we use mass spectrometry-based proteomic strategy to characterize protein expression in membrane-enriched fractions derived from human NK lymphoma cell line YTS. This query yielded a list of over 1000 identified proteins, and provided us with new insights on NK cell biology. We highlight the expression of CD86 on YTS and its ability to co-stimulate TCR responses of human CD4+ T-cells, providing an unexpected link between innate and adaptive immune systems.
Hanna
Jacob
J
The Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
Fitchett
Jonathan
J
Rowe
Tony
T
Daniels
Mark
M
Heller
Melissa
M
Gonen-Gross
Tsufit
T
Manaster
Efrat
E
Cho
Samuel Y
SY
LaBarre
Michael J
MJ
Mandelboim
Ofer
O
D015703:Antigens, CD / Q000378:metabolism*; D051940:B7-2 Antigen; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D011948:Receptors, Antigen, T-Cell / Q000276:immunology
D015703:Antigens, CD; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell