[Dynamic study of anti-NS5 and ALT in post-transfusion hepatitis C].
In order to study the character and dynamic changes of HCV NS5 antigen in post-transfusion hepatitis C, EIA was established with fusion protein, and different groups of population were investigated. The results showed the positive rate of whole blood donor, normal people, chronic hepatitis C patients and post transfusion hepatitis C patients is 0.0%, 1.66%, 50.7% and 70.5%, respectively. Serial blood samples of 25 cases of acute or chronic post-transfusion hepatitis C were detected, and the results indicated that the antibody of NS5 appeared relatively late, and the serum conversion time was 182.9 +/- 168.5 days. The anti-NS5 positive rate in 1, 3, 6, 12 and 24 months after HCV infection was 28%, 40%, 52%, 68% and 76%, respectively. The positive rate of anti-NS5 in sera with HCV RNA was 61.9%. Analysis on the appearance of antibody to the different antibody region of the gene, ALT and HCV RNA showed the dynamic changes of anti-NS5 were corresponding to the serum ALT in some cases, indicating antibody of NS5 appear later and may reflect the disease activity to some extent. The dynamic changes of anti-NS5 are of four kinds: transient positive, intermittent positive, persistent positive and persistent negative in during the two years period.
Xu
D
D
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
Lu
H
H
Nu
J
J
Meng
Z
Z
Shang
D
D
Kang
T
T
D000410:Alanine Transaminase / Q000097:blood*; D001803:Blood Transfusion; D006526:Hepatitis C / Q000201:enzymology* / Q000276:immunology* / Q000628:therapy / Q000821:virology; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D012324:RNA-Dependent RNA Polymerase
D000410:Alanine Transaminase; D018937:Hepatitis C Antibodies; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase