[Induction of chilling tolerance and heat shock protein synthesis in rice seedlings by heat shock].
Heat shock applied to germinated rice seeds increased the chilling tolerance of seedlings. Comparison with the control, brief heat shock applied before chilling at 4 degrees C for 2 days and recovery at 25 degrees C for another 2 days decreased the permeability of cellular membranes and increased the content of proline in rice seedlings. Heat shock applied before chilling also increased the activities of superoxide dismutase, catalase, and peroxidase and the content of ascorbate in rice seedlings. In contrast, the lipoxygenase activity and the malondialdehyde content in the heat-shocked rice seedlings were lower than those in the control. Germinated rice embryos synthesized heat shock proteins of Mr 78, 70, 64, 46, 38, 24, 17 and 16 kD during heat shock. The results of Western blot suggested that the binding protein (Bip) of HSP70 play an important role in protecting the rice seedling against chilling damage.
Huang
Shang-Zhi
SZ
Department of Bioscience and Biotechnology, Zhongshan University, Guangzhou 510275, China. lsshsz@zsu.edu.cn
Huang
Xiang-Fu
XF
Lin
Xiao-Dong
XD
Zhang
Yi-Shun
YS
Liu
Jun
J
Fu
Jia-Rui
JR
D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000096:biosynthesis; D003080:Cold Temperature; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D008715:Methionine / Q000378:metabolism; D012275:Oryza / Q000502:physiology*; D010940:Plant Proteins / Q000096:biosynthesis*; D036226:Seedlings / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D025801:Ubiquitin / Q000096:biosynthesis
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D025801:Ubiquitin; C077221:BIP protein, Arabidopsis; D008715:Methionine; D013482:Superoxide Dismutase