Role of seminal plasma in damage to turkey spermatozoa during in vitro storage.
In vitro storage of turkey spermatozoa is performed without consideration of the potential role of seminal plasma on sperm functions. We report the effects of seminal plasma on membrane permeability, lipid metabolism, energy status, motility and fertility of turkey spermatozoa stored at 4 or 20 degrees C. Phospholipid content (1077 nmol/10(9) spz versus 1219 nmol/10(9) spz at 48 h) and membrane permeability of spermatozoa were significantly damaged by the presence of seminal plasma after 48 h of storage at 4 degrees C, whereas damage to ATP content and fertility occurred earlier damaged by this presence (fertility after 24h storage 51% with seminal plasma versus 71% without). At 20 degrees C, seminal plasma decreased the phospholipid content of spermatozoa in the first hour of storage (1326 nmol/10(9) spz versus 1636 nmol/10(9) spz). Twenty-four hours later, this effect was masked by intense lipid peroxidation. These results show that seminal plasma is deleterious to storage of turkey spermatozoa at 4 degrees C and is involved in phospholipid metabolism of spermatozoa. Lipid peroxidation could be responsible for the acceleration of the degradation of sperm phospholipids during storage at 20 degrees C. However, lipid peroxidation seems not to be active at 4 degrees C. In this case, we suggest that phospholipase activation may contribute to sperm degradation, especially in the presence of seminal plasma.
Douard
Véronique
V
Station de Recherches Avicoles, INRA, F-37380 Nouzilly, France.
Hermier
Dominique
D
Labbe
Catherine
C
Magistrini
Michèle
M
Blesbois
Elisabeth
E
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002463:Cell Membrane Permeability; D004734:Energy Metabolism; D005298:Fertility; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D013696:Temperature; D014422:Turkeys
D010743:Phospholipids; D008315:Malondialdehyde; D000255:Adenosine Triphosphate