Localization of endothelin receptor subtype-A in the testis of rats, dogs, and monkeys.
In order to evaluate the physiological roles of the testicular endothelin (Edn) signaling via Edn receptor subtype-A (Ednra) in mammals, the localization of Ednra was investigated by in situ hybridization and immunohistochemistry in the testis of rats, dogs, and monkeys. For in situ hybridization, a rat Ednra RNA probe which is highly homologous to the subcloned canine and monkey Ednra (88.7% and 87.9% identical, respectively) was used. Both Ednra mRNA and protein were detected in interstitial cells and cells in the basal compartment of the seminiferous tubules, mainly Sertoli cells, as well as spermatogonia and some early spermatocytes, but not spermatids. The localization pattern of Ednra was exhibited in a same manner among species, indicating that the physiological role of Edn signaling throughout Ednra was maintained in the mammalian testis.
Okada
Akinobu
A
Safety Research Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., Tokyo, Japan.
Shishido
Takao
T
Nakano
Kenji
K
Ito
Kyoko
K
Tamura
Kotaro
K
Fujiwara
Michio
M
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D004285:Dogs; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007985:Leydig Cells / Q000737:chemistry; D008252:Macaca fascicularis; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D044022:Receptor, Endothelin A / Q000032:analysis*; D012671:Seminiferous Tubules / Q000737:chemistry / Q000166:cytology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000737:chemistry*
D044022:Receptor, Endothelin A