title: HLA class II haplotypes in Mexican systemic lupus erythematosus patients.
abstract: Systemic lupus erythematosus (SLE) is an autoimmune disease in which polymorphisms within the human leukocyte antigen (HLA) region have been associated to its etiology. For this study, HLA-DQB1, DQA1, and DRB1 genes were typed by polymerase chain reaction-sequence-specific primer in 237 individuals, taken from 74 families, who had a member with SLE, and who had their residence in the western region of Mexico; as well as in 159 ethnically matched healthy volunteers taken from 32 families. Genotype and allele frequency analysis was performed in 74 SLE patients and 54 unrelated controls. Precise three-loci identification of independent haplotypes was performed in 48 patients and 54 controls by familial segregation. Genotype distribution at each loci was concordant with Hardy-Weinberg's equilibrium in the control group. In general, no genotype effect was observed in SLE patients. Allele distribution comparison showed in the SLE group a significant increase of HLA-DQA1*0102, DQB1*0402, and DRB1*15; whereas alleles HLA-DQB1*0303 and *0501 were significantly decreased. SLE patients showed haplotype DQB1*0602-DQA1-*0102-DRB1*15 increased. As expected, patients with SLE have a reduced haplotype genetic diversity. The associations found in this study are related to an ancestral haplotype that has been observed in SLE populations of different origins.
authors.affiliation: Division of Immunology, HCMNO Instituto Mexicano del Seguro Social, Guadalajara, Mexico. CortesL@nei.nih.gov
authors.forename: Lizette M
authors.initials: LM
authors.lastname: Cortes
authors.forename: Luz M
authors.initials: LM
authors.lastname: Baltazar
authors.forename: Maria G
authors.initials: MG
authors.lastname: Lopez-Cardona
authors.forename: Norma
authors.initials: N
authors.lastname: Olivares
authors.forename: Cesar
authors.initials: C
authors.lastname: Ramos
authors.forename: Mario
authors.initials: M
authors.lastname: Salazar
authors.forename: Lucila
authors.initials: L
authors.lastname: Sandoval
authors.forename: Matthias G O
authors.initials: MG
authors.lastname: Lorenz
authors.forename: Ranajit
authors.initials: R
authors.lastname: Chakraborty
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Paterson
authors.forename: Fernando
authors.initials: F
authors.lastname: Rivas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D005838:Genotype; D006681:HLA-D Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008800:Mexico; D008875:Middle Aged
chemical_list: D006681:HLA-D Antigens; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains