title: The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
abstract: Diesel exhaust particles, and polystyrene particles (PSP) as a model for the insoluble particle core, have an adjuvant effect on allergen-specific IgE production in mice. We therefore examined the primary immune response in the draining popliteal lymph node (PLN) to the allergen ovalbumin (OVA) injected together with polystyrene particles into the footpad of BALB/cA mice. Similar numbers of particle-containing cells were observed in the draining lymph node on day 1 after injection of PSP alone or OVA + PSP, the numbers increasing continuously until day 21. The total lymph node cell numbers increased three to four times in the OVA + PSP group compared to both OVA and PSP groups, peaking on day 5. The increase in B cell numbers was twice the increase in T cell numbers. On day 5, OVA + PSP increased the expression of most surface markers measured (MHC class II, CD86, CD23, CD69) compared to OVA and PSP. Further, the ex vivo production of IL-4 and IL-10 by PLN cells from OVA + PSP-injected animals was increased. In conclusion, whereas PSP alone did not influence any of the immunologic markers studied, the adjuvant effect of PSP on the IgE antibody response to OVA was associated with an early increased primary cellular response in the draining lymph node.
authors.affiliation: Department of Environmental Immunology, Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway. unni.cecile.nygaard@fhi.no
authors.forename: Unni Cecilie
authors.initials: UC
authors.lastname: Nygaard
authors.forename: Heidi
authors.initials: H
authors.lastname: Ormstad
authors.forename: Audun
authors.initials: A
authors.lastname: Aase
authors.forename: Martinus
authors.initials: M
authors.lastname: Løvik
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D018899:CpG Islands / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D016130:Immunophenotyping; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010047:Ovalbumin / Q000276:immunology*; D011137:Polystyrenes / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D001335:Vehicle Emissions
chemical_list: D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D011137:Polystyrenes; D001335:Vehicle Emissions; D007073:Immunoglobulin E; D010047:Ovalbumin