title: Protein damage in drop-on-demand printers.
abstract: Inkjet printing technology is used to synthesize microarrays consisting of a variety of compounds. In this communication, we characterize damage to a model enzyme, peroxidase, caused by the rapid compression experienced by the solution during the printing process. We also find that damage is mitigated by the addition of trehalose and glucose to the printed solution.
authors.affiliation: H & N Instruments, Inc., PO Box 4338, Newark, Ohio 43055, USA. HNI@Infinet.com
authors.forename: Gary M
authors.initials: GM
authors.lastname: Nishioka
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Markey
authors.forename: Charles K
authors.initials: CK
authors.lastname: Holloway
mesh_terms: D004795:Enzyme Stability; D019793:Fluorescein / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D010544:Peroxidases / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*
chemical_list: D014199:Trehalose; D010544:Peroxidases; D005947:Glucose; D019793:Fluorescein