Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing.
A pyrosequencing method for detection and quantification of macrolide resistance mutations at positions 2058 and 2059 (Escherichia coli numbering) of the 23S rRNA gene is described. The method was developed and tested for Streptococcus pneumoniae, Streptococcus pyogenes, Mycobacterium avium, Campylobacter jejuni, and Haemophilus influenzae.
Haanperä
Marjo
M
Human Microbial Ecology Laboratory, National Public Health Institute, Kiinamyllynkatu 13, FI-20520 Turku, Finland. marjo.haanpera@ktl.fi
Huovinen
Pentti
P
Jalava
Jari
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D011756:Diphosphates / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D020459:Genes, rRNA; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D011756:Diphosphates; D018942:Macrolides; D012338:RNA, Ribosomal, 23S