Cloning and expression pattern of a novel microspore-specific gene encoding hypersensitive-induced response protein (LjHIR1) from the model legume, Lotus japonicus.
In order to understand the microspore and pollen development, recently, we have isolated a number of anther-specific genes in the model legume, Lotus japonicus. From these anther-specific genes, we identified one novel microspore-specific gene, LjImfb-c82. In order to determine the molecular characterization of LjImfb-c82, full-length cDNA clone was first isolated and sequenced. It encoded a protein of 286 amino acids (LjHIR1), which had sequence similarity to Hypersensitive-Induced Response like protein. LjHIR1 was specifically expressed in microspore on the in situ hybridization experiment. From the sequence similarity to prohibitin-domain protein, the LjHIR1 might be related to ion channel regulation in microspore development.
Hakozaki
Hirokazu
H
Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka 020-8550, Japan.
Endo
Makoto
M
Masuko
Hiromi
H
Park
Jong-In
JI
Ito
Hitoshi
H
Uchida
Masanori
M
Kamada
Motoshi
M
Takahashi
Hideyuki
H
Higashitani
Atsushi
A
Watanabe
Masao
M
D000595:Amino Acid Sequence; D035264:Flowers / Q000235:genetics* / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D000070116:Lotus / Q000235:genetics* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011058:Pollen / Q000502:physiology; D000091402:Prohibitins; D012097:Repressor Proteins / Q000235:genetics
D010940:Plant Proteins; D000091402:Prohibitins; D012097:Repressor Proteins