Norepinephrine in mice inhibits secretion of splenic IL-6 during the dark period but stimulates its secretion in the light period--possible role of the corticosterone tone.
In early morning hours, a rise of serum cytokines such as IL-6 was described. This study aimed to find reasons for this phenomenon focussing on NE and corticosterone. Mouse spleen slices were electrically stimulated (ES) in a microsuperfusion chamber in order to release endogenous NE. ES inhibited IL-6 secretion when animals were sacrificed at 03:00 and 06:00 (both p<0.001) but it increased its secretion from spleen slice removed at 09:00 (p=0.026). Prior administration of corticosterone or the glucocorticoid antagonist RU486 abrogated these ES effects. Endogenous NE via alpha- and beta-adrenoceptors mediated this time-dependent differential effects. This study demonstrates that cooperation of endogenous NE and corticosterone are involved in a time-dependent fall or rise of splenic IL-6 secretion.
Pongratz
Georg
G
Department of Molecular Virology, Immunology, and Medical Genetics, Ohio State University, Columbus, USA.
Härle
Peter
P
Schnellinger
Georg
G
Janele
David
D
Schölmerich
Jürgen
J
Straub
Rainer H
RH
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation / Q000379:methods; D005260:Female; D006727:Hormone Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D015735:Mifepristone / Q000494:pharmacology; D009248:Nadolol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology*; D010477:Perfusion; D010646:Phentolamine / Q000494:pharmacology; D017440:Photoperiod; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D018709:Statistics, Nonparametric; D013997:Time Factors; D015016:Yohimbine / Q000494:pharmacology
D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D006727:Hormone Antagonists; D015850:Interleukin-6; D015016:Yohimbine; D015735:Mifepristone; D009248:Nadolol; D003345:Corticosterone; D009638:Norepinephrine; D010646:Phentolamine