Detection of receptor activator of NF-kappa beta ligand in apical periodontitis.
A novel protein known as receptor activator of NF-kappa beta ligand (RANK-L) has been identified as a potential osteoclast differentiation factor. However, comparatively little is known about the expression of RANK-L in the pathogenesis of periradicular lesions. In this study, RANK-L expression was evaluated in biopsy specimens from apical periodontitis. Tissue samples from 21 periapical lesions were collected. RANK-L mRNA was isolated by using the guanidinium isothiocyanate acid phenol method. This was followed by generation of cDNA using specific primer for RANK-L by reverse transcription polymerase chain reaction amplification. RANK-L expression in each periapical sample was assessed by agarose gel electrophoresis. Gel electrophoresis analysis showed a single band of 385 bp corresponding to RANK-L in every periapical lesion. By contrast, normal control tissue showed no detectable RANK-L mRNA expression. In conclusion, RANK-L may play a role in apical periodontitis-induced bone resorption.
Sabeti
M
M
Division of Surgical Therapeutic and Bioengineering Science, School of Dentistry, University of Southern California, Los Angeles, CA , USA. sabeti2001@yahoo.com
Simon
J
J
Kermani
V
V
Valles
Y
Y
Rostein
I
I
D016301:Alveolar Bone Loss / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D016328:NF-kappa B / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism; D010485:Periapical Periodontitis / Q000378:metabolism*; D053245:RANK Ligand; D012333:RNA, Messenger / Q000032:analysis; D053246:Receptor Activator of Nuclear Factor-kappa B; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; C505688:TNFSF11 protein, human