A polyclonal antibody directed against syringylpropane epitopes of native lignins.
With a view to visualizing the ultrastructural distribution of syringyl lignins in secondary plant cell walls, a polyclonal antibody raised from a synthetic DHP polymer consisting only of syringyl propane units was prepared. To test the reactivity of the antiserum, a mini-dot-blot immunoassay reducing the amounts of substrates and antiserum was developed. A characteristic attribute of the S-antiserum appears to be its specific recognition of sequences of three or more consecutive syringyl units. On ultra-thin sections of model plants of Arabidopsis thaliana, Populus and tobacco, the antiserum allowed us to demonstrate a higher concentration of syringyl epitopes in fibres than in vessels. Variations in the distribution pattern of these epitopes between the three plants examined suggest that the synthesis of syringyl lignins in angiosperms depends on the species.
Joseleau
Jean-Paul
JP
Centre de recherche sur les macromolécules végétales, UPR CNRS 5301, BP53, 38041 Grenoble, France.
Faix
Oskar
O
Kuroda
Ken-Ichi
K
Ruel
Katia
K
D000911:Antibodies, Monoclonal; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure*; D000939:Epitopes; D008031:Lignin / Q000032:analysis / Q000276:immunology*; D018514:Plant Structures / Q000737:chemistry / Q000648:ultrastructure*; D011407:Propane
D000911:Antibodies, Monoclonal; D000939:Epitopes; C434469:syringyl monolignol; D008031:Lignin; D011407:Propane