title: Hepatoprotective and antibacterial effects of extracts from Trichilia emetica Vahl. (Meliaceae).
abstract: Trichilia emetica Vahl. (Meliaceae) is a tree widely distributed in Tropical Africa. It has been used in Mali folk medicine for the treatment of various illnesses. The aim of this work was to study the hepatoprotective and antibacterial effects of a crude aqueous extract from Trichilia emetica root. An ethyl ether fraction from the aqueous extract was also prepared and studied. We have examined the hepatoprotective activity of the extracts on CCl4-induced damage in rat hepatocytes, their toxicity using the brine shrimp bioassay and their antibacterial activity against clinical isolated bacterial strains, which are commonly responsible for respiratory infections. A preliminary phytochemical analysis showed a high polyphenolic content in the aqueous extract and the presence of limonoids in the ethyl ether fraction. These latter compounds may be considered responsible for the good activity against the bacterial strains tested. Trichilia emetica extracts exerted also a significant (P<0.05) hepatoprotective effect at a dose of 1000 microg/ml both on plasma membrane and mitochondrial function as compared to silymarin used as a positive control. These activities may be a result of the presence of either polyphenols or limonoids. Finally, both the aqueous extract and its ethyl ether fraction did not show toxicity (LC50>1000 microg/ml) in the brine shrimp bioassay.
authors.affiliation: Pharmaco-Biological Department, School of Pharmacy, University of Messina, Vill. SS. Annunziata, 98168 Messina, Italy. germamp@pharma.unime.it
authors.forename: M P
authors.initials: MP
authors.lastname: Germanò
authors.forename: V
authors.initials: V
authors.lastname: D'Angelo
authors.forename: R
authors.initials: R
authors.lastname: Sanogo
authors.forename: S
authors.initials: S
authors.lastname: Catania
authors.forename: R
authors.initials: R
authors.lastname: Alma
authors.forename: R
authors.initials: R
authors.lastname: De Pasquale
authors.forename: G
authors.initials: G
authors.lastname: Bisignano
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology / Q000517:prevention & control; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D006193:Haemophilus influenzae / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D016488:Medicine, African Traditional; D029582:Meliaceae; D008826:Microbial Sensitivity Tests; D001936:Moraxella catarrhalis / Q000187:drug effects; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D020011:Protective Agents / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013211:Staphylococcus aureus / Q000187:drug effects; D013296:Streptococcus pneumoniae / Q000187:drug effects; D013297:Streptococcus pyogenes / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D010636:Phenols; D010936:Plant Extracts; D020011:Protective Agents