[Preliminary studies on proliferation of hemorrhagic fever with renal syndrome virus in Leptotrombidium (L.) Scutellare].
In order to observe further the proliferation and kinetic changes of hemorrhagic fever with renal syndrome virus (HFRSV) in Leptotrombidium (L.) Scutellare infected with HFRSV, the raised Leptotrombidium (L.) Scutellare which were at larve stage or at nymph stage were grinded and sterilized by filtration at interval of 20 days, each batch of the filtrate was inoculated separately to Vero-E6 cells and the titre (TCID50/ml) of HFRSV was measured. The results confirmed: HFRSV were isolated from each batch of larve besides the batch at the 60th days, and the titre of HFRSV was -10(-1)-10(-4); HFRSV were also isolated from two batches of nymph. The isolated HFRSV were amplified by PCR technique and the HFRSV RNA were positive. The above results give rise to evidence that Leptotrombidium (L.) Scutellare might be the transmission vector of HFRSV.
Zhang
Y
Y
Nanjing Institute of Military Medicine, Nanjing Command, Nanjing 210002.
Li
X
X
Zhu
J
J
Tang
J
J
Li
Y
Y
Wu
G
G
Zhang
J
J
Jiang
K
K
Gan
Y
Y
Zhou
Y
Y
Tian
J
J
D000818:Animals; D001096:Arachnid Vectors / Q000821:virology*; D002522:Chlorocebus aethiops; D018063:Hantaan virus / Q000502:physiology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000635:transmission* / Q000821:virology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014324:Trombiculidae / Q000821:virology*; D014709:Vero Cells