title: Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly-roll fold.
abstract: We determined the three-dimensional crystal structure of the protein YML079wp, encoded by a hypothetical open reading frame from Saccharomyces cerevisiae to a resolution of 1.75 A. The protein has no close homologs and its molecular and cellular functions are unknown. The structure of the protein is a jelly-roll fold consisting of ten beta-strands organized in two parallel packed beta-sheets. The protein has strong structural resemblance to the plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) but also to some plant and bacterial enzymes (epimerase, germin). The protein forms homodimers in the crystal, confirming measurements of its molecular mass in solution. Two monomers have their beta-sheet packed together to form the dimer. The presence of a hydrophobic ligand in a well conserved pocket inside the barrel and local sequence similarity with bacterial epimerases may suggest a biochemical function for this protein.
authors.affiliation: Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, Centre National de la Recherche Scientifique-Unité Mixte de Recherche 8619, Université Paris-Sud, 91405 Orsay, France.
authors.forename: Cong-Zhao
authors.initials: CZ
authors.lastname: Zhou
authors.forename: Philippe
authors.initials: P
authors.lastname: Meyer
authors.forename: Sophie
authors.initials: S
authors.lastname: Quevillon-Cheruel
authors.forename: Inès
authors.initials: I
authors.lastname: Li De La Sierra-Gallay
authors.forename: Bruno
authors.initials: B
authors.lastname: Collinet
authors.forename: Marc
authors.initials: M
authors.lastname: Graille
authors.forename: Karine
authors.initials: K
authors.lastname: Blondeau
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: François
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leulliot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sorel
authors.forename: Anne
authors.initials: A
authors.lastname: Poupon
authors.forename: Joel
authors.initials: J
authors.lastname: Janin
authors.forename: Herman
authors.initials: H
authors.lastname: Van Tilbeurgh
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C500136:CFF1 protein, S cerevisiae