Measurement of hepatic functional mass by means of 13C-methacetin and 13C-phenylalanine breath tests in chronic liver disease: comparison with Child-Pugh score and serum bile acid levels.
OBJECTIVE
To evaluate and compare the clinical usefulness of 13C-phenylalanine and 13C-methacetin breath tests in quantitating functional hepatic mass in patients with chronic liver disease and to further compare these results with those of conventional tests, Child-Pugh score and serum bile acid levels.


METHODS
One hundred and forty patients (50 HCV-related chronic hepatitis, 90 liver cirrhosis patients) and 40 matched healthy controls were studied. Both breath test and routine liver test, serum levels of cholic and chenodeoxycholic acid conjugates were evaluated.


RESULTS
Methacetin breath test, expressed as 60 min cumulative percent of oxidation, discriminated the hepatic functional capacity not only between controls and liver disease patients, but also between different categories of chronic liver disease patients. Methacetin breath test was correlated with liver function tests and serum bile acids. Furthermore, methacetin breath test, as well as serum bile acids, were highly predictive of Child-Pugh scores. The diagnostic power of phenylalanine breath test was always less than that of methacetin breath test.


CONCLUSIONS
Methacetin breath test represents a safe and accurate diagnostic tool in the evaluation of hepatic functional mass in chronic liver disease patients.
Festi
D
D
Department of Internal Medicine and Gastroenterology, University of Bologna, Bologna, Italy. festi@med.unibo.it
Capodicasa
S
S
Sandri
L
L
Colaiocco-Ferrante
L
L
Staniscia
T
T
Vitacolonna
E
E
Vestito
A
A
Simoni
P
P
Mazzella
G
G
Portincasa
P
P
Roda
E
E
Colecchia
A
A
D000081:Acetamides / Q000493:pharmacokinetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001647:Bile Acids and Salts / Q000097:blood*; D001944:Breath Tests; D002247:Carbon Isotopes / Q000009:adverse effects; D005260:Female; D006521:Hepatitis, Chronic / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010649:Phenylalanine / Q000493:pharmacokinetics*; D011379:Prognosis; D012680:Sensitivity and Specificity
D000081:Acetamides; D001647:Bile Acids and Salts; D002247:Carbon Isotopes; C035495:methacetin; D010649:Phenylalanine