Mathematical basis for the optimization of single cell-line screening on multi-well plates.
Screening of stably transfected cells on multi-well plates is most efficient when a maximum number of wells contain a single colony. The multinomial distribution has been used to derive a novel equation that relates the number of wells containing a specified number of cell-lines and the total number of viable cells loaded on the plate. To test its validity, Chinese hamster ovary cells were transfected with a gene coding for the M2 muscarinic cholinergic receptor and screened on 96-well plates. The observed and predicted numbers of wells containing a single cell-line were indistinguishable. This approach therefore can be used to optimize the conditions for screening transfected cells.
Ma
Dengbo
D
Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 19 Russell St., Toronto, Ontario, M5S 2S2, Canada. dengbo.ma@utoronto.ca
Wells
James W
JW
D000465:Algorithms; D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival / Q000502:physiology*; D015169:Colony Count, Microbial / Q000379:methods*; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D008954:Models, Biological; D015233:Models, Statistical; D043585:Receptor, Muscarinic M2 / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D018401:Sample Size; D016008:Statistical Distributions; D014162:Transfection / Q000379:methods*
D043585:Receptor, Muscarinic M2; D011994:Recombinant Proteins