In vitro susceptibilities of bloodstream isolates of Candida species to six antifungal agents: results from a population-based active surveillance programme, Barcelona, Spain, 2002-2003.
OBJECTIVE
The antifungal drug susceptibilities of 351 isolates of Candida species, obtained through active laboratory-based surveillance in the period January 2002-December 2003, were determined (Candida albicans 51%, Candida parapsilosis 23%, Candida tropicalis 10%, Candida glabrata 9%, Candida krusei 4%).


METHODS
The MICs of amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole and caspofungin were established by means of the broth microdilution reference procedure of the European Committee on Antibiotic Susceptibility Testing.


CONCLUSIONS
Amphotericin B and flucytosine were active in vitro against all strains. A total of 24 isolates (6.8%) showed decreased susceptibility to fluconazole (MIC > or = 16 mg/L) and 43 (12.3%) showed decreased susceptibility to itraconazole (MIC > or = 0.25 mg/L). Voriconazole and caspofungin were active in vitro against the majority of isolates, even those that were resistant to fluconazole.
Cuenca-Estrella
Manuel
M
Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid. mcuenca-estrella@isciii.es
Rodriguez
Dolores
D
Almirante
Benito
B
Morgan
Juliette
J
Planes
Ana Maria
AM
Almela
Manel
M
Mensa
José
J
Sanchez
Ferran
F
Ayats
Josefina
J
Gimenez
Montserrat
M
Salvado
Margarita
M
Warnock
David W
DW
Pahissa
Albert
A
Rodriguez-Tudela
Juan L
JL
D000935:Antifungal Agents / Q000494:pharmacology*; D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification* / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data / Q000639:trends; D011159:Population Surveillance / Q000379:methods; D013030:Spain / Q000453:epidemiology
D000935:Antifungal Agents