[MIRU-VNTR genotyping of Mycobacterium tuberculosis strains from East Siberian: Beijing family versus Kilimanjaro family].
Seventy-two M. tuberculosis strains isolated from patients in different regions of East Siberia were studied by MIRU-VNTR repeats. Phylogenetic trees of relationship between the studied strains and of those from other world regions were investigated by phylogenetic analysis, i.e. NJ, UPGMA and ML. Phylogenetic evidence is provided for the prevalence of Beijing and Beijing-like strains (71%) in the above region. Besides, there was found a group of strains (115%) with the robust genotype which was given the name of ASU (African-Siberian-Ural). A linkage between MIRU10 (5-10 repeat) and MIRU26 (single repeat) was established as a predominant ASU sign. According to preliminary results, apart from Siberia, ASU strains can be encountered in the Urals and Africa (Tanzania, Kilimanjaro genotype). MIRU-VNTR consensus of ASU for East Siberia is 22( > 6)225113233.
Medvedeva
T V
TV
Ogarkov
O B
OB
Nekipelov
O M
OM
Ushakov
I V
IV
Koz'iakova
E S
ES
Skvortsova
R G
RG
D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012800:Siberia / Q000453:epidemiology; D020080:Tandem Repeat Sequences; D014376:Tuberculosis / Q000453:epidemiology*
D004269:DNA, Bacterial