[An experimental study of effect of different extracts of Alisma orientalis on urinary calcium oxalate stones formation in rats].
OBJECTIVE
To study the effect of different extracts of Alisma orientalis on urinary calcium oxalate stone formation in rats and to identify the effective constituents.


METHODS
Different extracts were administered through a stomach tube to rats of different groups with renal calcium oxalate stones induced by ethylene glycol (EG) and ammonium chloride (AC).


RESULTS
In the rats administered with ethyl acetate elution of ethyl acetate extract, blood Cr, BUN, renal tissue calcium content, urinary calcium excretion and crystals deposition in renal tissue were significantly lower than those of the stone formation group.


CONCLUSIONS
The ethyl acetate elution of ethyl acetate fraction extract of Alisma orientalis can significantly inhibit urinary calcium oxalate stone formation in rats and be the most effective constituent of Alisma orientalis.
Cao
Zheng-guo
ZG
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. zgcao2002@hotmail.com
Liu
Ji-hong
JH
Radman
A M
AM
Wu
Ji-zhou
JZ
Ying
Chun-ping
CP
Zhou
Si-wei
SW
D034902:Alisma / Q000737:chemistry; D000643:Ammonium Chloride; D000818:Animals; D001806:Blood Urea Nitrogen; D002118:Calcium / Q000378:metabolism; D002129:Calcium Oxalate / Q000652:urine*; D003404:Creatinine / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D019855:Ethylene Glycol; D007668:Kidney / Q000378:metabolism*; D007669:Kidney Calculi / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D008274:Magnesium / Q000378:metabolism / Q000652:urine; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D000643:Ammonium Chloride; D002129:Calcium Oxalate; D003404:Creatinine; D019855:Ethylene Glycol; D008274:Magnesium; D002118:Calcium