Ectoine accumulation in Brevibacterium epidermis.
As a halotolerant bacterial species, Brevibacterium epidermis DSM 20659 can grow at relatively high salinity, tolerating up to 2 M NaCl. It synthesizes ectoine and the intracellular content increases with the medium salinity, with a maximum of 0.14 g ectoine/g CDW at 1 M NaCl. Sugar-stressed cells do not synthesize ectoine. Ectoine synthesis is also affected by the presence of external osmolytes. Added betaine is taken up and completely replaced ectoine, while L-proline is only temporarily accumulated after which ectoine is synthesized. The strain can metabolize ectoine; L-glutamate is a better carbon source for ectoine synthesis than L-aspartate.
Onraedt
Annelies
A
Department of Biochemical and Microbial Technology, Ghent University, Ghent, Belgium.
De Muynck
Cassandra
C
Walcarius
Bart
B
Soetaert
Wim
W
Vandamme
Erick
E
D000599:Amino Acids, Diamino / Q000378:metabolism*; D001951:Brevibacterium / Q000502:physiology*; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism; D049109:Cell Proliferation; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008657:Metabolic Clearance Rate; D009997:Osmotic Pressure; D014882:Water-Electrolyte Balance / Q000502:physiology*
D000599:Amino Acids, Diamino; D002244:Carbon; C045628:ectoine