[The effects of herb lithospermum extract on MCF-7 cell and estrogen and progestogen levels in mice].
OBJECTIVE
To study the effects of lithospermum extract on MCF-7 cell and estrogen and progestogen levels in mice.


METHODS
Cell growth curve and Western Blotting were used to do animal experiment.


RESULTS
Lithospermum extract could inhibit the growth of MCF-7 cell. It could also inhibit the expression of ER and increase the expression of PR with large dose. After the mice were bred with Lithospermum, their serum estrogen and progestogen levels reduced, their uterus weight index decresed and uterus ER and PR levels increased. It could also improve the hyperplasia of uterus caused by tamoxifen.


CONCLUSIONS
Lithospermum extract can inhibit the growth of MCF-7 cell and inhibit the level of estrogen and progestogen in mice.
Wang
Wei
W
Department of Combination of Traditional Chinese Medicine and Western Medicine, Oncology School of Peking University, Beijing 100036, China. beilou207@163.com
Li
Ping-ping
PP
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004967:Estrogens / Q000097:blood*; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000139:chemically induced; D029684:Lithospermum / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal / Q000737:chemistry; D011372:Progestins / Q000097:blood*; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D013629:Tamoxifen / Q000009:adverse effects; D014599:Uterus / Q000378:metabolism / Q000473:pathology
D004365:Drugs, Chinese Herbal; D004967:Estrogens; D011372:Progestins; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D013629:Tamoxifen