title: Microstructured arrays with pre-synthesized capture probes for DNA detection based on metal nanoparticles and silver enhancement.
abstract: DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal.
authors.affiliation: Institute for Physical High Technology, PO Box 100239, 07702 Jena, Germany.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
authors.forename: Robert
authors.initials: R
authors.lastname: Möller
authors.forename: Robert
authors.initials: R
authors.lastname: Kretschmer
authors.forename: Andrea
authors.initials: A
authors.lastname: Csáki
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fritzsche
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D012834:Silver / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D015342:DNA Probes; D012834:Silver; D006046:Gold; D004247:DNA