title: Production of Lactobacillus kefir cells for asymmetric synthesis of a 3,5-dihydroxycarboxylate.
abstract: An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7--9 mmol l(-1) h(-1). The cells exhibited good and highly stereoselective reducing activities against tert-butyl 6-chloro-3,5-dioxohexanoate. tert-Butyl (3R,5S)-6-chloro-dihydroxyhexanoate, a chiral building block for HMG-CoA reductase inhibitor synthesis, was produced with 47.5% yield and >99% ee at C(3)3 and C(5)5 in a simple batch biotransformation process.
authors.affiliation: Institute of Biochemical Engineering, Munich University of Technology, Boltzmannstrasse 15, 85748 Garching, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Pfruender
authors.forename: Maya
authors.initials: M
authors.lastname: Amidjojo
authors.forename: Florian
authors.initials: F
authors.lastname: Hang
authors.forename: Dirk
authors.initials: D
authors.lastname: Weuster-Botz
mesh_terms: D018533:Biomass; D003470:Culture Media; D006880:Hydroxy Acids / Q000378:metabolism*; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism*; D010100:Oxygen; D013237:Stereoisomerism
chemical_list: D003470:Culture Media; D006880:Hydroxy Acids; D010100:Oxygen