title: Base composition of Coffea AFLP sequences and their conservation within the genus.
abstract: Amplified fragment length polymorphism (AFLP) is often used for genetic mapping and diversity analysis, but very little information is currently available on their sequence characteristics. Species-specific sequences were analyzed from a single Coffea genome (Coffea pseudozanguebariae) associated with clustered or nonclustered AFLP loci of known genetic position. Compared with the expressed sequence tag (EST) sequence composition, their AT content exhibited a bimodal distribution with AT-poor sequences corresponding mainly to putative coding sequences. AT-rich sequences, apart from the EST distribution, were usually clustered on the genetic map and might correspond to noncoding sequences. Conversion of these AFLP markers into sequence-characterized amplified region (SCAR) anchor markers allowed us to assess sequence conservation within Coffea species with respect to species relatedness.
authors.affiliation: UMR 1097 Diversité et Génome des Plantes Cultivées, Centre IRD, 911 avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France. poncet@mpl.ird.fr
authors.forename: V
authors.initials: V
authors.lastname: Poncet
authors.forename: P
authors.initials: P
authors.lastname: Hamon
authors.forename: M-B Sauvage
authors.initials: MB
authors.lastname: de Saint Marc
authors.forename: T
authors.initials: T
authors.lastname: Bernard
authors.forename: S
authors.initials: S
authors.lastname: Hamon
authors.forename: M
authors.initials: M
authors.lastname: Noirot
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping; D040503:Coffea / Q000235:genetics*; D020224:Expressed Sequence Tags; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length