[Effect of magnesium supplementation on NO and NOS subtypes in cultured human umbilical vein endothelial Cells].
This study inquired into the effect of Mg2+ on nitric oxide (NO) and nitric oxide synthase (NOS) in cultured human umbilical vein endothelial cells.


METHODS
The N02- / NO3- content was measured by copper-gilted cadmiun reduction. The detection of eNOS and iNOS expression was performed using immunohistochemistry techniques. This study consisted of 4 groups: normal control; H2O2; Mg2+; and H2O2 + Mg2+.


RESULTS
The NO2- / NO3- content in the group of H2O2 was lower than that in the normal control group (P < 0.05). The NO2- / NO3- contents in the group of H2O2 + Mg2+ at different doses were much higher than that in the group of H2O2 (P < 0.05). When compared with the normal control group, the average Grey levels of iNOS and NF-kappaB P65 in the group of H2O2 were significantly decreased (P < 0.05), and those of eNOS in the group of H2O2 and group of Mg2+ at different doses were increased (P < 0.05). In the group of H2O2 + Mg2+, the average Grey levels of eNOS were significantly decreased (P < 0.05), but those of iNOS and NF-kappaB P65 were increased as compared to the group of H2O2 (P < 0.01). The average Grey levels of eNOS and iNOS in the group of H2O2+ Mg2+ at differnet doses were lower than those in the group of Mg2+, respectively.


CONCLUSIONS
The antioxidant mechanisms of magnesium are probably involved in the regulation of cellular transcription factors and of NOS subtypes.
Lü
Xiaohua
X
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Ruishu
Wang
W
D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000201:enzymology*; D006801:Humans; D008274:Magnesium / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
D000975:Antioxidants; D009569:Nitric Oxide; C496319:NOS2 protein, human; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D008274:Magnesium