title: Gene expression profile of an adenomyoepithelioma of the breast with a reciprocal translocation involving chromosomes 8 and 16.
abstract: Myoepithelium is an integral part of the mammary ductal and lobular architecture, positioned between luminal cells and the basement membrane. We describe the first report on cytogenetic findings in an adenomyoepithelioma of the breast with a balanced t(8;16)(p23;q21), and provide gene expression profile using Affymetrix GeneChip U95AV2 (Affymetrix, Santa Clara, CA). Differential analysis identified 857 genes with 2-fold or more mRNA change in comparison to pooled normal breast control; immunohistochemical analysis was used to confirm these results in a limited number of genes. Expression results were grouped based on the chromosomal location of the genes and associated protein function, and identified several potential pathogenetic mechanisms (autocrine and paracrine growth stimuli) in the development of myoepithelial tumors.
authors.affiliation: Department of Pathology, Creighton University School of Medicine, 601 North 30th Street, Omaha, NE 68131-2197. zgatalica@pathology.creighton.edu
authors.forename: Zoran
authors.initials: Z
authors.lastname: Gatalica
authors.forename: Gopalrao
authors.initials: G
authors.lastname: Velagaleti
authors.forename: Helena
authors.initials: H
authors.lastname: Kuivaniemi
authors.forename: Gerard
authors.initials: G
authors.lastname: Tromp
authors.forename: Juan
authors.initials: J
authors.lastname: Palazzo
authors.forename: Kerry M
authors.initials: KM
authors.lastname: Graves
authors.forename: Michelle
authors.initials: M
authors.lastname: Guigneaux
authors.forename: Thomas
authors.initials: T
authors.lastname: Wood
authors.forename: Mala
authors.initials: M
authors.lastname: Sinha
authors.forename: Bruce
authors.initials: B
authors.lastname: Luxon
mesh_terms: D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16; D002898:Chromosomes, Human, Pair 8; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D009208:Myoepithelioma / Q000235:genetics*; D014178:Translocation, Genetic