title: Conservation of cis prolyl bonds in proteins during evolution.
abstract: In proteins and peptides, the vast majority of peptide bonds occurs in trans conformation, but a considerable fraction (about 5%) of X-Pro bonds adopts the cis conformation. Here we study the conservation of cis prolyl residues in evolutionary related proteins. We find that overall, in contrast to local, protein sequence similarity is a clear indicator for the conformation of prolyl residues. We observe that cis prolyl residues are more often conserved than trans prolyl residues, and both are more conserved than the surrounding amino acids, which show the same extent of conservation as the whole protein. The pattern of amino acid exchanges differs between cis and trans prolyl residues. Also, the cis prolyl bond is maintained in proteins with sequence identity as low as 20%. This finding emphasizes the importance of cis peptide bonds in protein structure and function.
authors.affiliation: Institute of Biochemistry, Charité Berlin, Berlin, Germany. stephan.lorenzen@charite.de <stephan.lorenzen@charite.de>
authors.forename: Stephan
authors.initials: S
authors.lastname: Lorenzen
authors.forename: Björn
authors.initials: B
authors.lastname: Peters
authors.forename: Andrean
authors.initials: A
authors.lastname: Goede
authors.forename: Robert
authors.initials: R
authors.lastname: Preissner
authors.forename: Cornelius
authors.initials: C
authors.lastname: Frömmel
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D019540:Area Under Curve; D005075:Biological Evolution; D017124:Conserved Sequence; D006801:Humans; D008956:Models, Chemical; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D010649:Phenylalanine / Q000737:chemistry; D011392:Proline / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
chemical_list: D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan; D011392:Proline