title: New SRM data dependent exclusion (MS)n measurement for structural determination of drug metabolites using LC/ESI/Ion trap MS.
abstract: New SRM (selected reaction monitoring) data dependent exclusion (MS)(n) measurement makes it possible to obtain MS(3) fragmentation data for all MS(2) fragments, useful for structural determination of drug metabolites using ESI ion trap. MS(2) fragments are produced by cleavage of all protonated molecules at the lone electron pairs of heteroatoms or the pi electrons of double and triple bonds, benzene rings and hetero-rings of drugs. Usually, data dependent MS(3) measurement cleaves only MS(2) fragment of highest intensity, that normally does not contain important metabolic sites. Fragmentation data from all parts of drug metabolites is required to determine structure. In addition to the usual basic measurement of protonated molecules and (MS)(n) fragmentation of drug metabolites, we demonstrate the use of SRM data dependent (MS)(n) measurement, plus new SRM data dependent exclusion (MS)(n) measurement for structural determination of metabolites.
authors.affiliation: Biopharmaceutical and Pharmacokinetics Research Laboratories Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan. zenzaburo_tozuka@po.fujisawa.co.jp
authors.forename: Zenzaburo
authors.initials: Z
authors.lastname: Tozuka
authors.forename: Hayato
authors.initials: H
authors.lastname: Kaneko
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Shiraga
authors.forename: Manabu
authors.initials: M
authors.lastname: Beppu
authors.forename: Toshiro
authors.initials: T
authors.lastname: Niwa
authors.forename: Akio
authors.initials: A
authors.lastname: Kawamura
authors.forename: Akira
authors.initials: A
authors.lastname: Kagayama