[Expression of P120 catenin in the cell lines and tissues of normal liver and hepatocellular carcinoma].
OBJECTIVE
To explore P120 catenin expression in normal liver cell line, hepatocellular carcinoma cell line, normal liver tissue and hepatocellular carcinoma tissues, further exploring the relationship-between catenin and hepatocellular carcinoma.


METHODS
Reverse transcript polymerase chain reaction (RT-PCR), immunofluorescence microscopy and immunohistochemistry methods were used to detect P120 catenin expression in the cells and tissues mentioned above.


RESULTS
RT-PCR results showed that P120 catenin 1A and 3A isoform mRNA can be detected in all the cells and tissues. Immunofluorescence microscopy and immunohistochemistry results showed that P120 catenin expressed at cell to cell contact region and at cell membrane in normal liver cells and in normal liver tissues, while in hepatocellular carcinoma cell lines and in hepatocellular carcinoma tissues, the molecule expression increased in cytosol, membrane expression reduced, and there was nuclear translocation.


CONCLUSIONS
Hepatocellular carcinoma was found to have relation with abnormal P120 catenin expression in this study, which suggested the process of abnormal cell signaling and adhesion functions during the onset and development of hepatocellular carcinoma.
Nong
Chaozan
C
Department of Experiment Center, Clinical Institute of Guangxi Nationalities Hospital, Nanning 530001, China.
Guo
Lingxiao
L
Huang
Huayi
H
D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D051177:Catenins; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000095002:Delta Catenin
D014408:Biomarkers, Tumor; D051177:Catenins; D015815:Cell Adhesion Molecules; D010750:Phosphoproteins; D012333:RNA, Messenger; D000095002:Delta Catenin; C000722347:CTNND1 protein, human