[Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro].
OBJECTIVE
To study the expression of osteoprotegerin(OPG) and receptor activator nuclear factor kappa B ligand(RANKL) at protein level in human periodontal ligament cells (HPDLCs), and the effect of 1alpha,25(OH)(2) vitamin D(3) [1,25(OH)(2)vitD(3)] on the secretion of OPG protein in vitro.


METHODS
HPDLCs were harvested in vitro by sequential digestion with trypsin and collagenase. The expression of RANKL in HPDLCs at protein level was tested by immunocyto-chemistry. Enzyme-linked immunoadsordent assay (ELISA) was used to detect the OPG protein which was secreted into the culture medium by HPDLCs cultured with and without 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3) on the 0, 2nd, 4th, and 6th days, respectively.


RESULTS
RANKL protein was detected on the membrane and plasma of HPDLCs, and OPG protein was secreted in the culture medium. The secretion of OPG protein was down-regulated by 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3).


CONCLUSIONS
HPDLCs have the bone metabolism system of OPG/RANKL, which works during the process of 1alpha,25 (OH)(2)vitD(3) inducing HPDLCs. The conclusion has laid the groundwork for the study on bone remodelling mechanisms of HPDLCs.
Zhang
Ding
D
Department of Orthodontics, Peking University School of Stomatology, Beijing 100081, China. dingz@ht.rol.cn.net
Yang
Yan-qi
YQ
Li
Xiao-tong
XT
Fu
Min-kui
MK
D002112:Calcifediol / Q000494:pharmacology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D053244:Osteoprotegerin / Q000096:biosynthesis*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053245:RANK Ligand / Q000096:biosynthesis*
D053244:Osteoprotegerin; D053245:RANK Ligand; D002112:Calcifediol