A suite of novel human spermatozoal RNAs.
We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10,000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.
Ostermeier
G Charles
GC
Charlotte B. Failing Professor of Fetal Therapy and Diagnosis, 253 C.S. Mott Center, 275 East Hancock, Detroit, MI 48201, USA.
Goodrich
Robert J
RJ
Moldenhauer
Julie S
JS
Diamond
Michael P
MP
Krawetz
Stephen A
SA
D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis; D016372:RNA, Antisense; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000502:physiology*
D035683:MicroRNAs; D016372:RNA, Antisense