title: Evaluation of a high-density oligonucleotide array for characterization of grlA, grlB, gyrA and gyrB mutations in fluoroquinolone resistant Staphylococcus aureus isolates.
abstract: Using high-density oligonucleotide array technology, 30 Staphylococcus aureus strains were studied for the presence of mutations in genes involved in fluoroquinolone resistance: grlA, gyrA, grlB and gyrB. For the two most important genes, gyrA and grlA, correlation with sequencing reached 95.1%. If all genes were considered, correlation was 88.8%.
authors.affiliation: Division of Infectious Diseases, University Hospitals of Geneva, CH-1211, 24 rue Micheli-du-Crest, 1211 Geneva 14, Switzerland. corinne.jay@eu.biomerieux.com
authors.forename: Sabine
authors.initials: S
authors.lastname: Couzinet
authors.forename: Javier
authors.initials: J
authors.lastname: Yugueros
authors.forename: Christine
authors.initials: C
authors.lastname: Barras
authors.forename: Nathalie
authors.initials: N
authors.lastname: Visomblin
authors.forename: Patrice
authors.initials: P
authors.lastname: Francois
authors.forename: Bruno
authors.initials: B
authors.lastname: Lacroix
authors.forename: Guy
authors.initials: G
authors.lastname: Vernet
authors.forename: Daniel
authors.initials: D
authors.lastname: Lew
authors.forename: Alain
authors.initials: A
authors.lastname: Troesch
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
authors.forename: Corinne
authors.initials: C
authors.lastname: Jay
mesh_terms: D003062:Codon; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase