[Studies on the pharmacokinetics of lidamycin in mice and dogs using bioassay].
OBJECTIVE
A bioassay method was established for the determination of active concentrations of lidamycin and studied its pharmacokinetics in mice and dogs.


METHODS
Cytotoxicity of lidamycin in vitro was used to determine drug serum concentrations in vivo.


RESULTS
Validity of methodology met the requirements of pharmacokinetic study. The concentration-time profile in mice after iv lidamycin of 100, 50 and 10 microg x kg(-1) was best fitted with 2-compartmental model with T1/2alpha and T1/2beta of 0.77-1.8 min and 5.6-7.2 min, respectively. The AUC were 2851.3, 887.8 and 166.4 microg x min x L(-1), respectively and increased with dose nonlinearly. There were similar trends between AUC and the potency of tumor growth inhibition. After iv lidamycin of 12 microg x kg(-1) in dogs, the concentrations of lidamycin decreased rapidly and the AUC was 16 microg x min x L(-1), which were lower and quicker than those in mice. The levels in serum after second administration at day 15, were lower than those of the first.


CONCLUSIONS
Active concentrations and pharmacokinetics of lidamycin were obtained by bioassay method successfully. There are species differences and single and multi-dosing differences in the pharmacokinetics of lidamycin.
Chen
Shu-zhen
SZ
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Tang
Zhong-ming
ZM
Zhen
Yong-su
YS
D000617:Aminoglycosides / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D019540:Area Under Curve; D001681:Biological Assay; D004285:Dogs; D053281:Enediynes; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D007624:KB Cells / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D012510:Sarcoma 180 / Q000473:pathology; D013045:Species Specificity
D000617:Aminoglycosides; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; C058024:C 1027