Bxz1, a new generalized transducing phage for mycobacteria.
We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae. Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10(-8) to 10(-6) per plaque forming unit between strains of M. smegmatis. We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene.
Lee
Sunhee
S
Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Kriakov
Jordan
J
Vilcheze
Catherine
C
Dai
Zhiyan
Z
Hatfull
Graham F
GF
Jacobs
William R
WR
D003227:Conjugation, Genetic; D005819:Genetic Markers; D009160:Mycobacteriophages / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D009161:Mycobacterium / Q000235:genetics / Q000821:virology*; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000821:virology*; D010957:Plasmids; D012988:Soil Microbiology; D014161:Transduction, Genetic
D005819:Genetic Markers