Specific radiation damage illustrates light-induced structural changes in the photosynthetic reaction center.
The photosynthetic reaction center of the purple non-sulfur bacterium Blastochloris viridis was frozen in the presence and absence of illumination. Differences in the resulting datasets are monitored using the difference Fourier method. Radiation damage is localized to those parts of the protein that are significant for electron transfer, and show changes that are sensitive to oxidation and protonation state.
Baxter
Richard H G
RH
Department of Chemistry, University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA. r.baxter@alumni.uchicago.edu
Seagle
Brandon-Luke
BL
Ponomarenko
Nina
N
Norris
James R
JR
D020561:Alphaproteobacteria / Q000737:chemistry; D003580:Cytochromes / Q000737:chemistry; D005583:Fourier Analysis; D006418:Heme / Q000737:chemistry; D008027:Light; D008958:Models, Molecular; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000528:radiation effects*; D011487:Protein Conformation / Q000528:radiation effects; D012242:Rhodobacter sphaeroides / Q000737:chemistry; D014965:X-Rays
D003580:Cytochromes; D045322:Photosynthetic Reaction Center Complex Proteins; D006418:Heme