Developmental expression and nutritional regulation of a zebrafish gene homologous to mammalian microsomal triglyceride transfer protein large subunit.
The microsomal triglyceride transfer protein (MTP) large subunit is required for the assembly and secretion of apolipoprotein B-containing lipoproteins. We have found a zebrafish mtp homologous gene coding a protein with 54% identity with human MTP large subunit with the most conserved regions distributed in the corresponding predicted alpha-helical and C- and A-sheet domains. In situ hybridizations showed that zebrafish mtp transcripts were distributed in the yolk syncytial layer during early embryogenesis and in anterior intestine and liver from 48 hr postfertilization onward. Real-time quantitative RT-PCR confirmed the developmental regulation and tissue-specificity of mtp expression. A significant pretranslational up-regulation of mtp expression was observed in the anterior intestine after feeding. The nutritional regulation of zebrafish mtp expression observed in the anterior intestine supports the notion that this protein, similar to mammalian MTP large subunit, could be a factor implicated directly or indirectly in large lipid droplets accumulation observed in the fish enterocyte after feeding.
Marza
Esther
E
Laboratoire Génomique et Physiologie des Poissons, UMR 1067 NUAGE INRA-IFREMER, Université Bordeaux 1, 33405 Talence Cedex, France.
Barthe
Christophe
C
André
Michèle
M
Villeneuve
Laure
L
Hélou
Chantal
C
Babin
Patrick J
PJ
D000595:Amino Acid Sequence; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000502:physiology*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D020895:Enterocytes / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000737:chemistry; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013997:Time Factors; D014018:Tissue Distribution; D015854:Up-Regulation; D015027:Zebrafish
D002352:Carrier Proteins; D008074:Lipoproteins; D012333:RNA, Messenger; C106092:microsomal triglyceride transfer protein; D012313:RNA