Truncated and RIP-degenerated copies of the LTR retrotransposon Pholy are clustered in a pericentromeric region of the Leptosphaeria maculans genome.
The LMR1 5.2 kb interspersed repeat of Leptosphaeria maculans was described by Taylor and Borgmann [Mol. Plant Microbe Interact. 7 (1994) 181] as an uncharacterized repeated element sharing homologies with both LINEs and SINEs. Here, we used the LMR1 sequence as a template to identify the full-length element within a 184-kb genomic sequence corresponding to the pericentromeric region of the 2.80 Mb chromosome of isolate v23.1.3. This region comprises (i) one 6980-bp full-sized Pholy element bordered by two 275- to 280-bp long terminal repeats (LTRs), (ii) five Pholy-related sequences, usually truncated at their 3' ends, and (iii) five solo-LTRs. Structural features strongly suggested that Pholy corresponds to an ancient copia-like retrotransposon, sharing strong homologies with the Elsa retrotransposon of Stagonospora nodorum. Pholy was also suggested to be specific to pericentromeric regions. Comparative analysis of the structure of the Pholy-like sequences occurring in the 184-kb contig and in other parts of the genome showed that this family of repeats is highly degenerated following extensive repeat induced point mutation (RIP).
Attard
Agnès
A
INRA, PMDV, Route de Saint Cyr, F-78026 Versailles Cedex, France.
Gout
Lilian
L
Ross
Simon
S
Parlange
Francis
F
Cattolico
Laurence
L
Balesdent
Marie-Hélène
MH
Rouxel
Thierry
T
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics*; D002503:Centromere; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D005800:Genes, Fungal; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D017354:Point Mutation; D018626:Retroelements / Q000235:genetics*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences / Q000235:genetics*
D018626:Retroelements