Transfer of a gene containing the Arg-Gly-Asp peptide prolongs the bleeding time of mice.
Peptides containing Arg-Gly-Asp (RGD) have been used to decrease thrombosis by competitive inhibition of the integrin glycoprotein, alphaIIb/beta3a, in platelets. However, they have a short half-life in vivo. A naked plasmid, pCMV-RGD, was transferred into the skeletal muscle of mice and RGD gene expression was observed by RT-PCR. The bleeding time between control mice and RGD-transferred mice was prolonged from the 10th day to the 80th day after gene transfer while the blood glucose and serum insulin-like proteins remained at normal levels. These results provided a convenient and effective approach to relieve patients from thrombi in a single step over a relatively long period.
Chen
Weiqiang
W
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, P.R. China.
Liu
Jian
J
Diao
Weifei
W
Tang
Jianguo
J
Ji
Jianguo
J
D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage / Q000378:metabolism; D001760:Bleeding Time / Q000379:methods; D001777:Blood Coagulation / Q000502:physiology*; D004247:DNA / Q000008:administration & dosage*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism*; D009842:Oligopeptides / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism*; D013927:Thrombosis / Q000378:metabolism / Q000628:therapy
D000925:Anticoagulants; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D004247:DNA