[Screening of cDNA clone for putative RNA polymerase subunit of Cysticercus cellulosae].
OBJECTIVE
To obtain related genes of Cysticercus cellulosae from spliced leader (SL) cDNA library.


METHODS
Spliced leader library of Cysticercus cellulosae was constructed using SL specific primer and oligo (dT) 15 with M13M4 primer, and positive clones were then screened randomly, identified with enzyme restriction, followed by sequencing and homologous analysis.


RESULTS
The amino acid sequence, encoded by the positive clone with a poly (A)22 tail and a complete open reading frame (ORF), was with homology of RNA polymerase subunit genes of human, B. napus, fission yeast, A. thaliana, C. elegans and fruit fly up to 71.6%.


CONCLUSIONS
The protein, RNA polymerase subunit encoded putatively by the clone, is high conservative in different species.
Luo
Xue-nong
XN
Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
Zheng
Ya-dong
YD
Dou
Yong-xi
YX
Hou
Jun-lin
JL
Jing
Zhi-zhong
ZZ
Cai
Xue-peng
XP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003551:Cysticercosis / Q000469:parasitology*; D003552:Cysticercus / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020038:RNA, Spliced Leader / Q000235:genetics; D013552:Swine
D018076:DNA, Complementary; D012333:RNA, Messenger; D020038:RNA, Spliced Leader; D012321:DNA-Directed RNA Polymerases