Long-term productive human immunodeficiency virus infection of CD1a-sorted myeloid dendritic cells.
Myeloid, CD1a-sorted dendritic cells (MDC) productively replicated human immunodeficiency virus strains encoding envelope genes of either primary X4R5 or R5 strains for up to 45 days. Cell-free supernatant collected from long-term infected MDC, which had been exposed to an X4R5 virus 45 days earlier, was still infectious when placed over activated T cells. These data imply that DC can act as a persistent reservoir of infectious virus.
Popov
Sergei
S
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, Massachusetts 02115-6084, USA.
Chenine
Agnès-Laurence
AL
Gruber
Andreas
A
Li
Pei-Lin
PL
Ruprecht
Ruth M
RM
D018949:Antigens, CD1 / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D004197:Disease Reservoirs; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D022423:Myeloid Cells / Q000166:cytology*; D019713:Receptors, CCR5 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D013997:Time Factors; D014779:Virus Replication
D018949:Antigens, CD1; C419393:CD1a antigen; D019713:Receptors, CCR5; D019718:Receptors, CXCR4