Isomers of conjugated linoleic acid decrease plasma lipids and stimulate adipose tissue lipogenesis without changing adipose weight in post-prandial adult sedentary or trained Wistar rat.
The respective effects and interactions of supplementation with two conjugated linoleic acid (CLA) isomers and exercise on plasma metabolic profile, activity of lipogenic enzymes and cellularity in two adipose tissue sites, those of the liver and heart, were examined in adult Wistar rats. Rats that were either sedentary or exercise-trained by treadmill running were fed one of four diets: a diet without CLA; a diet with either 1% cis 9, trans 11 CLA or 1% trans 10, cis 12 CLA; or a mixture of both isomers (1% of each) for 6 weeks. We observed that the exercise decreased lipogenic enzyme activities in epididymal and perirenal adipose tissue. Plasma cholesterol, insulin, and leptin concentrations were lower in exercise-trained rats than in sedentary rats. The ingestion of either CLA mixture or the trans 10, cis 12 CLA increased lipogenic enzyme activities in epididymal tissue and more markedly in perirenal adipose tissue, especially in sedentary rats, and without affecting adipose tissue weight or cellularity. A similar effect of trans 10, cis 12 CLA was observed in regard to malic enzyme activity in the liver. In addition, this isomer decreased plasma lipid and urea concentrations and increased plasma 3-hydroxybutyrate levels. The ingestion of cis 9, trans 11 CLA increased fatty acid synthase activity in perirenal adipose tissue in sedentary rats and decreased plasma cholesterol and leptin concentrations. These results show that isomers of CLA decrease plasma lipids and stimulate adipose tissue lipogenesis without changing adipose weight in adult sedentary or exercise-trained rat, thus suggesting a stimulation of adipose tissue turnover.
Faulconnier
Yannick
Y
Herbivore Research Unit, Adipose Tissue and Milk Lipids Group, National Institute for Agricultural Research-Theix, 63122 Saint-Genès Champanelle, France.
Arnal
Marie-Agnès
MA
Patureau Mirand
Philippe
P
Chardigny
Jean-Michel
JM
Chilliard
Yves
Y
D000273:Adipose Tissue / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage; D064429:Fatty Acid Synthases / Q000378:metabolism; D005502:Food; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007536:Isomerism; D020738:Leptin / Q000097:blood; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis* / Q000097:blood*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D005082:Physical Exertion; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D007328:Insulin; D020738:Leptin; D044243:Linoleic Acids, Conjugated; D008055:Lipids; D002784:Cholesterol; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase