title: [Development of microchips for the detection of mutations of HIV-1 variability to protease inhibitors and the usage results].
abstract: An original biochip was constructed for the detection of 34 mutations of HIV-1 resistance to protease. A technology was worked out, which is based on the hybridization of a fluorescence-labeled amplified fragment of the pol gene of the HIV-1 provirus DNA with a set of specific oligonucleotides immobilized in 3-D hydrogel pads of the biological microchip. The biochip was used to analyze 115 samples of the subtype-1 provirus HIV-1 DNA isolated from untreated IDUs and their sexual partners in 15 regions of former USSR countries. Substitution of Val/IIe in position 77 of protease (V771) is known as secondary mutation of resistance to Nelfinavir detected in 55 (47.8%) of 115 HIV-1 variations. Its first appearance was registered in a patient with HIV in April 1997 in Tver, where its carrying variant caused an HIV outbreak. It is demonstrated that the V771-substitution variant, that dominates in Moscow, caused outbreaks in Irkutsk and Yekaterinburg and spread into separate districts of Perm and Perm Region. At the same time, no V771 HIV-1 was detected in any of the HIV studied cases diagnosed before 1998 in Moldova, Ukraine and Rostov Region.
authors.forename: N I
authors.initials: NI
authors.lastname: Rudinskiĭ
authors.forename: V M
authors.initials: VM
authors.lastname: Mikhaĭlovich
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Donnikov
authors.forename: S A
authors.initials: SA
authors.lastname: Lapa
authors.forename: A L
authors.initials: AL
authors.lastname: Sukhanova
authors.forename: E V
authors.initials: EV
authors.lastname: Kazennova
authors.forename: A F
authors.initials: AF
authors.lastname: Bobkov
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
authors.forename: V V
authors.initials: VV
authors.lastname: Pokrovskiĭ
authors.forename: A D
authors.initials: AD
authors.lastname: Mirzabekov
mesh_terms: D019943:Amino Acid Substitution; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks; D024882:Drug Resistance, Viral / Q000235:genetics*; D015751:Genes, pol; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D019888:Nelfinavir / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D011533:Proviruses / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D012747:Sexual Partners; D015819:Substance Abuse, Intravenous / Q000188:drug therapy / Q000453:epidemiology
chemical_list: D004279:DNA, Viral; D017320:HIV Protease Inhibitors; D015345:Oligonucleotide Probes; D019888:Nelfinavir