Conjugation of antibodies to fluorescein or rhodamine.
The isothiocyanate derivative of the fluorophore, fluorescein or rhodamine, is coupled to the amino groups of IgG antibody in a one-step procedure and excess label is removed by gel filtration.
Wisdom
G Brian
GB
School of Biology and Biochemistry, The Queen's University, Belfast, Northern Ireland, UK.
D000818:Animals; D002850:Chromatography, Gel; D019793:Fluorescein / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*
D007074:Immunoglobulin G; D012235:Rhodamines; D019793:Fluorescein