title: Identification of tumor-associated autoantigens with SEREX.
abstract: Serological analysis of tumor antigens by recombinant cDNA expression cloning (SEREX) allows the systematic cloning of tumor antigens recognized by the spontaneous autoantibody repertoire of cancer patients. For SEREX, cDNA expression libraries are constructed from fresh tumor specimens, packaged into lambda-phage vectors, and expressed recombinantly in Escherichia coli. Recombinant proteins expressed during the lytic infection of bacteria are transferred onto nitrocellulose membranes to be probed with diluted autologous patient serum for identification of clones reactive with high-titered IgG antibodies. This chapter describes the SEREX technology in detail.
authors.affiliation: Department of Internal Medicine, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Türeci
authors.forename: Dirk
authors.initials: D
authors.lastname: Usener
authors.forename: Sandra
authors.initials: S
authors.lastname: Schneider
authors.forename: Ugur
authors.initials: U
authors.lastname: Sahin
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D006801:Humans
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D001324:Autoantigens; D018076:DNA, Complementary