[Determination of four alkaloids in Berberis plants by HPLC].
OBJECTIVE
In order to assess Berberis, a simple and effective high-performance liquid chromatographic (HPLC) method was established.


METHODS
By applying HPLC with gradiation elution, this method was developed for determination of four kinds of alkaloids including berbamine, Jatrorrhizine, berberine and palmatine in methanolic extracts from root bark, root, stem bark and stem of Berberis.


RESULTS
The alkaloids berbamine, jatrorrhizine, berberine and palmatine shown good linear correlations in the range of 0.028-4.74 microg (r = 0.9998); 0.012-2.0 microg (r = 0.9996); 0.026-0.52 microg (r = 0.9999); 0.015-2.56 microg (r = 0.9998), respectively. The correlation coefficients of the calibration curve for the analytes exceeded 0.9998.


CONCLUSIONS
The optimized HPLC method was applied to analyze various samples, and the results shown that the content of alkaloids were obvious difference in Berberis from different area, different species and different parts.
Di
Duo-long
DL
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China. ddlliu@yahoo.com.cn
Liu
Ye-wei
YW
Ma
Zhi-gang
ZG
Jiang
Sheng-xiang
SX
D000470:Alkaloids / Q000032:analysis; D044182:Benzylisoquinolines / Q000032:analysis; D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis*; D028142:Berberis / Q000737:chemistry* / Q000145:classification; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D013045:Species Specificity
D000470:Alkaloids; D044182:Benzylisoquinolines; D001600:Berberine Alkaloids; C055785:jatrorrhizine; D001599:Berberine; C005413:palmatine; C027870:berbamine