title: Capturing SDS-treated biotinylated protein and peptide by avidin functional affinity electrophoresis with or without SDS in the gel running buffer.
abstract: Avidin functional affinity electrophoresis (AFAEP) is a new method of affinity electrophoresis. In this technique, bifunctional linker glutaraldehyde is added to the polyacrylamide gel solution to embed avidin within the gel matrix by interaction with the amino/amide groups. Samples are heated with triglycine sodium dodecyl sulfate (SDS) sample buffer to ensure that biotinylated proteins biotinylated peptides are negatively charged and migrate electrophoretically toward the cathode through the avidin zone regardless of their pI values. The AFAEP method allows the capture and concentration of biotinylated proteins or biotinylated peptides irrespective of the use of SDS in both the sample buffer and the gel running buffer.
authors.affiliation: Protein Research Laboratory, Research Resources Center, University of Illinois, Chicago, IL 60612, USA. boblee@uic.edu
authors.forename: Bao-Shiang
authors.initials: BS
authors.lastname: Lee
authors.forename: Shalini
authors.initials: S
authors.lastname: Gupta
authors.forename: Sangeeth
authors.initials: S
authors.lastname: Krishnanchettiar
authors.forename: Syed Salman
authors.initials: SS
authors.lastname: Lateef
mesh_terms: D000818:Animals; D001360:Avidin / Q000378:metabolism*; D019921:Biotinylation; D002021:Buffers; D002417:Cattle; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002021:Buffers; D010455:Peptides; D011506:Proteins; D001360:Avidin; D012967:Sodium Dodecyl Sulfate