Effects of 5-fluorouridine on modified nucleosides in mouse liver transfer RNA.
Administration of the pyrimidine antimetabolite, 5-fluorouridine, to mice was found to cause a marked specific reduction of the amounts of 5-methyluridine, pseudouridine, and dihydrouridine but not of 3-(3-amino-3-carboxypropyl)uridine in tRNA from the livers of the treated animals. The data presented indicate that this effect is not simply due to the incorporation of 5-fluorouridine into tRNA; the drug appears to interfere directly with the enzymic reactions involved in the modification of the 5-position of uridine. 5-Fluorouridine was found to have no effect on the modification of adenosine, guanosine, and cytidine in mouse liver tRNA.
Lu
L W
LW
Department of Pharmacology, Baylor College of Medicine, Texas Medical Center, Houston, Texas 77030, USA.
Chiang
G H
GH
Tseng
W C
WC
Randerath
K
K
D000818:Animals; D001482:Base Composition / Q000187:drug effects; D002855:Chromatography, Thin Layer; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D009705:Nucleosides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014529:Uridine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*
D009705:Nucleosides; C001943:5-fluorouridine; D012343:RNA, Transfer; D014529:Uridine