Study of antinociceptive effect of isolated fractions from Petiveria alliacea L. (tipi) in mice.
The acetate (FA), hexanic (FH), hydroalcoholic (FHA) and precipitated hydroalcoholic (FHAppt) fractions from the root of Petiveria alliacea L. were evaluated for antinociceptive effect using the abdominal constriction induced by acetic acid, hot-plate, formalin tests. The open field and rota rod tests were used to evaluate psychomotor function and myorelaxant activity. The fractions were administered intraperitoneally in mice at doses of 100 and 200 mg/kg. Inhibitions of abdominal constrictions were observed with all doses of the fractions, as compared to control. FH and FHAppt, at both doses, reduced the nociception produced by formalin in the 1st (0-5 min) and 2nd (20-25 min) phases, however FHA (100, 200 mg/kg) and FA 200 mg/kg presented significant inhibition on the 1st and 2nd phases, respectively, of this test. A reduction of the locomotor activity was observed in the open field test with all the fractions. These fractions failed to affect the motor coordination in the rota rod test. Results showed that the different fractions of Petiveria alliacea L. have different antinociceptive potentials as demonstrated in the experimental models of nociception in mice, supporting folk medicine use of this plant.
Gomes
Patrícia Bezerra
PB
Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil.
Oliveira
Maria Mirele da Silva
MM
Nogueira
Carlos Renato Alves
CR
Noronha
Emmanuelle Coelho
EC
Carneiro
Lyvia Maria Vasconcelos
LM
Bezerra
José Noberto Sousa
JN
Neto
Manoel Andrade
MA
Vasconcelos
Silvania Maria Mendes
SM
Fonteles
Marta Maria França
MM
Viana
Glauce Socorro Barros
GS
de Sousa
Francisca Clea Florenço
FC
D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D005260:Female; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects* / Q000379:methods; D029602:Phytolaccaceae; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D045442:Rotarod Performance Test / Q000379:methods
D000700:Analgesics; D010936:Plant Extracts