[Cloning and sequencing of p35 gene from Bombyx mori nuclear polyhedrosis virus strain Shandong].
According to the 5' and 3' untranslated region sequences of p35 gene from Bombyx mori nuclear polyhedrosis virus isolate T3, a pair of primers was designed employing computer analysis. With the primers,polymerase chain reaction (PCR) was performed and a 1080 bp fragment was obtained from BmNPV strain Shandong. Sequencing result showed that it was p35 gene (GenBank accession number: AY157746), and it included an open reading frame of 900 bp, encoding 299 amino acids with Mr=34.91 kDa and pI=6.40. BLAST analysis indicated that there were seven bases and three amino acids difference between p35 from BmNPV strain Shandong and that from BmNPV isolate T3.
Jiang
Li-Hua
LH
Department of Sericultural Silk Sciences, South China Agricultural University, Guangzhou 510642, China.
Zhong
Wan-Fang
WF
Cai
Ping-Zhong
PZ
Xu
Xing-Yao
XY
Yan
Wen-Zhao
WZ