[The germinating characters of the transgenic rice seeds in the stress condition of antibiotic G418 and their application in crop breeding].
The seeds of transgenic rice line D2-1-2 and the receptor cultivar Zhonghua No.9 were germinated on the stress condition of the antibiotic G418. The number of taking root seed, the length of root and the length of shoot of two used materials were checked in different concentrations of the antibiotic G418, but the ratio of germinating seed was not affected. At the 100 mg/L level of G418, the transgenic line D2-1-2 could take longer root (mean 1.45 cm) but Zhonghua No.9 very short (mean 0.27 cm). 88.46% of the total long-root (<0.5 cm) seeds selected from the mixing population of D2-1-2 and Zhonghua No.9 at the 100 mg/L level of antibiotic G418 were real transgenic ones.
Xu
Ming-Hui
MH
Institute of Biotechnology, Yunnan Agricultural Academy of Sciences, Kunming 650223, China.
Tang
Zuo-Shun
ZS
Zhao
Feng-Ping
FP
Tian
Wen-Zhong
WZ