Separation of B6 vitamers with micellar liquid chromatography using UV and electrochemical detection.
Separation of six vitamers of vitamin B6 was performed by RP-HPLC using micellar mobile phase, UV and electrochemical detection. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency and asymmetry factor showed that, the appropriate conditions were temperature of 35 degrees C and 3.0-5.0% (v/v) 1-butanol in mobile phase. Variations of selectivity factor versus 1-butanol concentration, pH of mobile phase, and SDS concentration was investigated and the following optimized conditions were selected for the separation: 3.0% (v/v) 1-butanol, pH=5.5 and 65 mM SDS in mobile phase. Electrochemical behavior of vitamers in optimized mobile phase was investigated using cyclic voltammetry, and potential of +1.2 V versus Ag/AgCl(Sat.) was chose as working potential. Finally, separation of B6 vitamers using UV detection at 254 nm and electrochemical detection at +1.2 V was compared.
Hadjmohammadi
Mohammad Reza
MR
Chemistry Department, Mazandran University, PO Box 453, Babolsar, Iran.
Momenbeik
Fariborz
F
Khorasani
Jafar Hoseinalizade
JH
D020001:1-Butanol / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D010084:Oxidation-Reduction; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D013997:Time Factors; D025101:Vitamin B 6 / Q000737:chemistry / Q000302:isolation & purification*
D008823:Micelles; D012967:Sodium Dodecyl Sulfate; D025101:Vitamin B 6; D020001:1-Butanol