title: A novel beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer.
abstract: A new member of the UDP-N-acetylglucosamine: beta-galactose beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T) family having the beta3-glycosyltransferase motifs was identified using an in silico method. This novel beta3Gn-T was cloned from a human colon cancer cell line and named beta3Gn-T8 based on its position in a phylogenetic tree and enzymatic activity. Beta3Gn-T8 transfers GlcNAc to the non-reducing terminus of the Galbeta1-4GlcNAc of tetraantennary N-glycan in vitro. HCT15 cells transfected with beta3Gn-T8 cDNA showed an increase in reactivity to both LEA and PHA-L4 in a flow cytometric analysis. These results indicated that beta3Gn-T8 is involved in the biosynthesis of poly-N-acetyllactosamine chains on tetraantennary (beta1,6-branched) N-glycan. In most of the colorectal cancer tissues examined, the level of beta3Gn-T8 transcript was significantly higher than in normal tissue. Beta3Gn-T8 could be an enzyme involved in the synthesis of poly-N-acetyllactosamine on beta1-6 branched N-glycans in colon cancer.
authors.affiliation: Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Central-2 OSL, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ishida
authors.forename: Akira
authors.initials: A
authors.lastname: Togayachi
authors.forename: Tokiko
authors.initials: T
authors.lastname: Sakai
authors.forename: Toshie
authors.initials: T
authors.lastname: Iwai
authors.forename: Toru
authors.initials: T
authors.lastname: Hiruma
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.forename: Reiko
authors.initials: R
authors.lastname: Okubo
authors.forename: Niro
authors.initials: N
authors.lastname: Inaba
authors.forename: Takashi
authors.initials: T
authors.lastname: Kudo
authors.forename: Masanori
authors.initials: M
authors.lastname: Gotoh
authors.forename: Junichi
authors.initials: J
authors.lastname: Shoda
authors.forename: Naomi
authors.initials: N
authors.lastname: Tanaka
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics; D019295:Computational Biology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics* / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D011134:Polysaccharides; D012333:RNA, Messenger; C037199:poly-N-acetyllactosamine; C496333:B3GNT8 protein, human; D017351:N-Acetylglucosaminyltransferases