Localisation of epidermal growth factor receptor in mucous cells of human salivary glands.
EGFR activation has been related to an increase in synthesis and secretion of mucins in epithelial cells, so that the use of EGFR tyrosine kinase inhibitors has been proposed in the therapy of mucin hypersecretory diseases. In this paper, we describe the ultrastructural localisation of EGFR in the mucous elements of human major and minor salivary glands and relate it to mucin distribution. A post-embedding immunogold staining method has been applied to normal surgical samples of human submandibular, sublingual, and labial glands, using a mouse monoclonal antibody specific for the intracellular domain of human EGFR. In mucous cells of all the glands examined, specific reactivity was detected in the cytoplasmic basolateral portions and near the mucous droplets, but not on cell surfaces. Since this pattern of labelling must be related to the internalisation process of the ligand-GFR complex, our results support the hypothesis that EGFR activation takes place in mucous cells and affects mucin production in human salivary glands.
Piludu
M
M
Dipartimento di Citomorfologia, Università di Cagliari, Cagliari, Italy. mpiludu@unica.it
Lantini
M S
MS
Isola
M
M
Puxeddu
R
R
Cossu
M
M
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D020397:Goblet Cells / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D012469:Salivary Glands / Q000737:chemistry* / Q000648:ultrastructure; D012470:Salivary Glands, Minor / Q000737:chemistry / Q000648:ultrastructure; D013361:Sublingual Gland / Q000737:chemistry / Q000648:ultrastructure; D013363:Submandibular Gland / Q000737:chemistry / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D066246:ErbB Receptors