[Preparation and characterization of recombinant retroviral vector containing t-PA cDNA].
A recombinant retroviral vector containing tissue-type plasminogen activator (t-PA) cDNA was constructed and transfected into PA317 viral packaging cells, forming intact virus particles. Under electron microscope the recombinant retroviral particles were composed of envelope, capsid and core. These viral particles were spherical with a diameter of 90-180nm, and spread dispersely in the cells. NIH3T3 cell infected by retrovirus particles were screened with G418. The virus titer of 6 x 10(8) CFU/L was verified by counting the positive clones two weeks after screening. The expression of t-PA was demonstrated in the NIH3T3 cells infected with the recombinant virus.
Cui
N
N
Department of Molecular Genetics, School of Basic Medical Sciences, Shanghai Medical University, Shanghai 200032.
Peng
L
L
Song
H
H
Han
Q
Q
Chen
H
H
D018076:DNA, Complementary / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D012190:Retroviridae / Q000235:genetics* / Q000648:ultrastructure; D010959:Tissue Plasminogen Activator / Q000235:genetics*
D018076:DNA, Complementary; D010959:Tissue Plasminogen Activator