Application of RAPD and SCAR markers for purity testing of F1 hybrid seed in chili pepper (Capsicum annuum).
A simpler and better method for purity testing of hybrid pepper seed was developed. The simplest method for extracting genomic DNA, the NaOH method, was chosen. Two RAPD markers identifying male and female parents were also developed, and the PCR products of male- and female-specific RAPD markers were cloned and sequenced. From these sequences, new longer primers were constructed for conversion into SCAR markers. In blind tests the RAPD and SCAR markers were able to reliably detect contaminating exotic seeds. These PCR-based markers are therefore directly applicable for purity testing by seed companies. In addition, the PCR products of the SCAR markers could be identified by direct staining methods such as ethidium bromide and pellet painting without electrophoresis.
Jang
Inok
I
Division of Plant Science and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, Korea.
Moon
Ji-Hye
JH
Yoon
Jae Bok
JB
Yoo
Jae-Heung
JH
Yang
Tae Jin
TJ
Kim
Yong Jae
YJ
Park
Hyo Guen
HG
D002212:Capsicum / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis*; D004996:Ethidium / Q000494:pharmacology; D017343:Genes, Plant; D005819:Genetic Markers; D005821:Genetic Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers; D004996:Ethidium