Efficient generation of 2'-deoxyuridin-5-yl at 5'-(G/C)AA(X)U(X)U-3' (X = Br, I) sequences in duplex DNA under UV irradiation.
The photoreactivity of 5-halouracil-containing DNA was investigated using 450 base pair DNA fragments under 302 nm irradiation. Heat-dependent cleavage selectively occurs at 5'-(G/C)AAXUXU-3' and 5'-(G/C)AXUXU-3' (X = Br, I) sequences in double-stranded DNA. HPLC product analysis indicated that 2'-deoxyribonolactone residues are effectively generated at these sequences. These observations will be useful in studying the molecular basis of the sequence-dependent DNA-damaging process in UV-irradiated 5-halouracil-containing DNA.
Watanabe
Takayoshi
T
School of Biomedical Science, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
Bando
Toshikazu
T
Xu
Yan
Y
Tashiro
Ryu
R
Sugiyama
Hiroshi
H
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004249:DNA Damage; D003856:Deoxyuracil Nucleotides / Q000737:chemistry*; D006846:Hydrocarbons, Halogenated / Q000737:chemistry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays; D014498:Uracil / Q000031:analogs & derivatives*
D003856:Deoxyuracil Nucleotides; D006846:Hydrocarbons, Halogenated; D009841:Oligonucleotides; D014498:Uracil; D004247:DNA; C007267:2'-deoxyuridylic acid