title: Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay.
abstract: A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.
authors.affiliation: Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944-0848, USA. lzsak@piadc.ars.usda.gov
authors.forename: L
authors.initials: L
authors.lastname: Zsak
authors.forename: M V
authors.initials: MV
authors.lastname: Borca
authors.forename: G R
authors.initials: GR
authors.lastname: Risatti
authors.forename: A
authors.initials: A
authors.lastname: Zsak
authors.forename: R A
authors.initials: RA
authors.lastname: French
authors.forename: Z
authors.initials: Z
authors.lastname: Lu
authors.forename: G F
authors.initials: GF
authors.lastname: Kutish
authors.forename: J G
authors.initials: JG
authors.lastname: Neilan
authors.forename: J D
authors.initials: JD
authors.lastname: Callahan
authors.forename: W M
authors.initials: WM
authors.lastname: Nelson
authors.forename: D L
authors.initials: DL
authors.lastname: Rock
mesh_terms: D000357:African Swine Fever / Q000175:diagnosis*; D000358:African Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D015342:DNA Probes; D014066:Palatine Tonsil / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine / Q000821:virology; D019914:Taq Polymerase
chemical_list: D015342:DNA Probes; D019914:Taq Polymerase