The protective role of tiopronin in cisplatin ototoxicity in Wistar rats.
The purpose of this study was to evaluate cisplatin-induced ototoxicity and the protective effects of tiopronin. Twenty-four adult Wistar rats served as subjects and were divided into three groups. Eight rats receiving only saline (group A) were used as controls. Eight rats received cisplatin (2 mg/kg) injections (group B) and eight rats received cisplatin and tiopronin (300 mg/kg) (group C) for 8 consecutive days. Both ears of all animals were tested by DPOAE before treatment and on the 4th and 9th days. Seventy-two hours after the final recording session, all animals were killed, and the left cochleas were prepared for electron microscopy and analysed. DPOAE responses were significantly reduced in group B compared to controls (p<0.05). When tiopronin was added, DPOAE responses were significantly increased compared to those obtained with the administration of cisplatin alone (p<0.05). The cochleogram showed that tiopronin had a significant protective effect in the basal half and in the lower half of the middle turn. We conclude that tiopronin, a drug effective in protecting against cisplatin nephrotoxicity, is also effective in protecting against cisplatin ototoxicity.
Fetoni
Anna Rita
AR
Institute of Otolaryngology, Catholic University of Rome, Rome, Italy. afetoni@unicatt.it
Quaranta
Nicola
N
Marchese
Raffaella
R
Cadoni
Gabriella
G
Paludetti
Gaetano
G
Sergi
Bruno
B
D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D016022:Case-Control Studies; D002945:Cisplatin / Q000009:adverse effects*; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000648:ultrastructure; D034381:Hearing Loss / Q000139:chemically induced / Q000517:prevention & control*; D008855:Microscopy, Electron, Scanning; D017084:Otoacoustic Emissions, Spontaneous; D051381:Rats; D017208:Rats, Wistar; D008625:Tiopronin / Q000008:administration & dosage*; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D008625:Tiopronin; D002945:Cisplatin