DNA-binding properties of cosmomycin D, an anthracycline with two trisaccharide chains.
Cosmomycin D (CosD) is the major constituent fraction isolated from a culture of Streptomyces olindensis ICB20. The ability of this compound to intercalate with double-stranded DNA was studied by gel mobility shift assays and electrospray ionization mass spectrometry (ESI-MS). ESI-MS experiments showed that the complex of CosD with 16-mer double-stranded DNA was at least as stable as a complex of daunorubicin with the same DNA sequence. This is the first study showing DNA binding properties of an anthracycline containing a beta-rhodomycinone aglycone chromophore O-linked to two trisaccharide chains.
Furlan
Renata L A
RL
Institute of Biomedical Sciences, University of Sao Paulo, SP, CEP 005508-900, Brazil.
Watt
Stephen J
SJ
Garrido
Leandro M
LM
Amarante-Mendes
Gustavo P
GP
Nur-e-alam
Mohammad
M
Rohr
Jurgen
J
Braña
Alfredo
A
Mendez
Carmen
C
Salas
Jose A
JA
Sheil
Margaret M
MM
Beck
Jennifer L
JL
Padilla
Gabriel
G
D018943:Anthracyclines / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D021241:Spectrometry, Mass, Electrospray Ionization
D018943:Anthracyclines; D004247:DNA; C052002:cosmomycin D