Antioxidant activity and protective effect on DNA strand scission of Rooibos tea (Aspalathus linearis).
Rooibos tea (Aspalathus linearis) was extracted by refluxing with water and 75% ethanol as a solvent. Antioxidant activity and protective effect on DNA strand scission were investigated by using different antioxidant assay systems and DNA strand nicking assay, respectively. 75% Ethanol extract has higher content of total soluble phenolics and flavonoid than water extract. Antioxidant activities such as hydrogen donating capacity and scavenging activity of hydrogen peroxide were higher in 75% ethanol extract than in water extract except the rate constant with hydroxyl radical. Peroxyl radical induced DNA strand scission was prevented by both 75% ethanol and water extract and hydroxyl radical induced DNA strand scission was not. This result indicates that total soluble phenolics, specially flavonoid, of Rooibos tea are responsible for several kinds of antioxidant activities and preventive activity on peroxyl radical induced DNA strand scission.
Lee
Eun-Jung
EJ
Department of Food and Nutrition, Hannam University, Daejeon, Korea.
Jang
Hae-Dong
HD
D000975:Antioxidants / Q000494:pharmacology*; D036903:Aspalathus; D001628:Beverages; D001713:Biphenyl Compounds; D004249:DNA Damage / Q000187:drug effects*; D005419:Flavonoids / Q000032:analysis; D007202:Indicators and Reagents; D010636:Phenols / Q000032:analysis; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects*; D013662:Tea
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013662:Tea; C004931:1,1-diphenyl-2-picrylhydrazyl