Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes.
Dehalococcoides ethenogenes is the only bacterium known to reductively dechlorinate the groundwater pollutants, tetrachloroethene (PCE) and trichloroethene, to ethene. Its 1,469,720-base pair chromosome contains large dynamic duplicated regions and integrated elements. Genes encoding 17 putative reductive dehalogenases, nearly all of which were adjacent to genes for transcription regulators, and five hydrogenase complexes were identified. These findings, plus a limited repertoire of other metabolic modes, indicate that D. ethenogenes is highly evolved to utilize halogenated organic compounds and H2. Diversification of reductive dehalogenase functions appears to have been mediated by recent genetic exchange and amplification. Genome analysis provides insights into the organism's complex nutrient requirements and suggests that an ancestor was a nitrogen-fixing autotroph.
Seshadri
Rekha
R
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. rekha@tigr.org
Adrian
Lorenz
L
Fouts
Derrick E
DE
Eisen
Jonathan A
JA
Phillippy
Adam M
AM
Methe
Barbara A
BA
Ward
Naomi L
NL
Nelson
William C
WC
Deboy
Robert T
RT
Khouri
Hoda M
HM
Kolonay
James F
JF
Dodson
Robert J
RJ
Daugherty
Sean C
SC
Brinkac
Lauren M
LM
Sullivan
Steven A
SA
Madupu
Ramana
R
Nelson
Karen E
KE
Kang
Katherine H
KH
Impraim
Marjorie
M
Tran
Kevin
K
Robinson
Jeffrey M
JM
Forberger
Heather A
HA
Fraser
Claire M
CM
Zinder
Stephen H
SH
Heidelberg
John F
JF
D000596:Amino Acids / Q000096:biosynthesis; D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000235:genetics* / Q000378:metabolism*; D020440:Gene Duplication; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006859:Hydrogen / Q000378:metabolism; D008969:Molecular Sequence Data; D009591:Nitrogenase / Q000235:genetics / Q000378:metabolism; D009876:Operon; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011809:Quinones / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013750:Tetrachloroethylene / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism
D000596:Amino Acids; D011809:Quinones; D014157:Transcription Factors; D014874:Water Pollutants, Chemical; D006859:Hydrogen; D010088:Oxidoreductases; C112990:tetrachloroethene dehalogenase; D009591:Nitrogenase; D013750:Tetrachloroethylene