[Gene localization and tissue expression of a novel human reproduction-related gene].
Gene localization is significant in elucidating the interaction between genes,gene and diseases. Using radiation hybrid (RH) technique,we cloned and localized a novel gene,designated human BSP-related protein (HBRP) on 19q13.2 - 13.3, in line with its localization in data bank of overlapping fragment of human genome through bioinformatics method. It is suggested RH is rapid, precise,simple and powerful in gene localization. In addition, we detected the expression and distribution of HBRP in human tissues by RT-PCR. The results showed HBRP was highly expressed in intestine, kidney, liver, spleen, stomach and pancreas, whereas lowly in brain, lung, muscle and heart.
Luo
Yang
Y
The Research Center for Medical Genomics and MOH Key Laboratory of Cell Biology, China Medical University, Shenyang 110001, China. luoyangjj@sina.com
Yu
Bing-Zhi
BZ