title: Investigation of retention on bare silica using reversed-phase mobile phases at elevated temperatures.
abstract: The use of unbonded silica as a stationary phase in reversed-phase HPLC is described as a useful alternative to bonded phase columns for polar, lipophilic amines. Using four lipophilic amines, the role of temperature is shown to favorably impact both efficiency and selectivity, which is not universally seen when using bonded phases. As temperature is raised, retention drops on the silica column. The temperature behavior appears to support the hypothesis that retention is dependant upon electrostatic and adsorptive forces.
authors.affiliation: Agilent Technologies, Little Falls Site, 2850 Centerville Road, Wilmington, DE 19808, USA. brian_bidlingmeyer@agilent.com
authors.forename: Brian A
authors.initials: BA
authors.lastname: Bidlingmeyer
authors.forename: John
authors.initials: J
authors.lastname: Henderson
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
chemical_list: D012822:Silicon Dioxide