[The effect of antisense human Fas RNA on activation induced apoptosis of T cell].
OBJECTIVE
To explore an approach to the reduction of activation induced apoptosis of T cell so as to maximize the number of CTL and enhance the tumor cytotoxicity.


METHODS
CD3-induced Jurkat cell line was used as the activated T cell apoptosis model,and the antisense Fas cDNA was introduced into Jurkat cells with retroviral vector.


RESULTS
The expression level of Fas protein in Jurkat cells transducted with the retroviral vector was decreased. Apoptosis was reduced in antisense Fas-transfected Jurkat cells after anti-CD3 or anti-Fas treatment.


CONCLUSIONS
Antisense blocking of Fas expression can partially inhibit Jurkat cell apoptosis induced by anti-CD3 or anti Fas.
Qian
Q
Q
Tumor Immunology and Gene Therapy Center, Eastern Institute of Hepatobiliary Surgery, Shanghai 200433.
Wu
M
M
Cao
H
H
Guo
Y
Y
Fang
S
S
Qu
Z
Z
Huang
H
H
Guo
Y
Y
D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D049109:Cell Proliferation; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D016372:RNA, Antisense / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D014162:Transfection; D019014:fas Receptor / Q000235:genetics*
C491901:FAS protein, human; D016372:RNA, Antisense; D019014:fas Receptor