title: Production of nitric oxide by hypoxic radiosensitizer sanazole.
abstract: OBJECTIVE
  In this work we investigated the ability of hypoxia-selective radiosensitizer--sanazole to produce nitric oxide (NO).
  
  
  METHODS
  NO formation was determined by spectophotometric method in the reaction with sanazole and oxyhemoglobin. In suspensions of lymphoma EL-4 and mastocytoma P 8815 cell NO production was estimated indirectly as nitrite concentration in the supernatant fraction.
  
  
  RESULTS
  Transformation of oxyhemoglobin by sanazole to methemoglobin suggested the dissociation of nitro group in aqueous solution and denitration of molecules. Addition of sanazole to hypoxic tumor cell suspension resulted in the increase of nitrite content in tissue culture medium.
  
  
  CONCLUSIONS
  Presented data suggest the ability of sanazole to produce NO that may be important in a probable mechanism for antitumor and immunomodulating properties of this radiosensitizer.
authors.affiliation: Institute of Oncology, Russian Academy of Medical Sciences, Tomsk, Russia. oncology@info.tsu.ru
authors.forename: Irina V
authors.initials: IV
authors.lastname: Kondakova
authors.forename: Varvara V
authors.initials: VV
authors.lastname: Tcheredova
authors.forename: Gelena V
authors.initials: GV
authors.lastname: Zagrebelnaya
authors.forename: Nadezda V
authors.initials: NV
authors.lastname: Cherdyntseva
authors.forename: Tsutomu V
authors.initials: TV
authors.lastname: Kagiya
authors.forename: Evgeny L
authors.initials: EL
authors.lastname: Choinzonov
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D008223:Lymphoma / Q000473:pathology*; D034801:Mastocytoma / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental; D009569:Nitric Oxide / Q000032:analysis*; D010108:Oxyhemoglobins / Q000378:metabolism; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D010108:Oxyhemoglobins; D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D009569:Nitric Oxide