Testosterone influenced the expression of Notch1, Notch2 and Jagged1 induced by lipopolysaccharide in macrophages.
Testosterone plays an extensive role in modulating macrophages functions in mammals. In this study, we incubated murine bone marrow macrophages which were positive to the surface antigen F4/80 with lipopolysaccharide and increasing amounts of testosterone. Expression of Notch family members (including Notch1, Notch2 and Jagged1) was investigated at transcription and post-transcription levels through RT-PCR and Western blotting assay followed by densitometric analyses. Results showed that testosterone influenced the lipopolysaccharide-induced expression of Notch1, Notch2 and Jagged1 in macrophages. The elevated expression of Notch1 and Notch2 induced by lipopolysaccharide was repressed by testosterone at lower levels, but enhanced at higher hormone levels. In addition, the expression of Jagged1 was enhanced by various amounts of testosterone. These results suggest that testosterone affected the expression of Notch family members in activated macrophages.
Guo
Dongxing
D
School of Basic and Forensic Medicine, Sichuan University, 610041 Chengdu, PR China.
Zhang
Huaping
H
Liu
Limin
L
Wang
Lianyun
L
Cheng
Yu
Y
Qiao
Zhongdong
Z
D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D015870:Gene Expression; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072099:Serrate-Jagged Proteins; D013739:Testosterone / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D017931:DNA Primers; D004338:Drug Combinations; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008070:Lipopolysaccharides; D008565:Membrane Proteins; C497762:Notch1 protein, mouse; C497765:Notch2 protein, mouse; D012333:RNA, Messenger; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors; D013739:Testosterone