Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility.
alpha2,8-Sialyltransferase (alpha2,8S-T, GD3 synthase) has been reported to be involved in the enhanced cell proliferation of malignant tumors. Using a cloned cDNA of alpha2,8S-T, transfectant cells were established and the effects of the gene expression on the cell phenotypes were analyzed. In contrast with PC12 cells, in which we reported marked growth enhancement based on the transfection of alpha2,8S-T, Swiss3T3 cells showed no enhancement in either cell proliferation or phosphorylation of MAP kinases after the transfection of alpha2,8S-T when treated with platelet-derived growth factor. Correspondingly, the receptor for platelet-derived growth factor also showed no increased phosphorylation upon the factor stimulation. However, in the wound-healing scratching assay, the Swiss3T3 transfectant cells demonstrated increased mobility as the PC12 transfectant cells. These results suggest that the enhancing effects of alpha2,8S-T on the proliferation and mobility are differential depending on the cell types, and ganglioside-associating molecules in the individual cell types need to be investigated.
Kamimura
Yutaka
Y
Department of Biochemistry II, Nagoya University School of Medicine, Nagoya 466-0065, Japan.
Furukawa
Keiko
K
Kittaka
Daiji
D
Nishio
Masashi
M
Hamamura
Kazunori
K
Fukumoto
Satoshi
S
Furukawa
Koichi
K
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry; D005732:Gangliosides / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D015151:Immunoblotting; D047468:Immunoprecipitation; D020935:MAP Kinase Signaling System; D051379:Mice; D008954:Models, Biological; D016716:PC12 Cells; D010641:Phenotype; D010766:Phosphorylation; D051381:Rats; D012799:Sialyltransferases / Q000494:pharmacology*; D041701:Swiss 3T3 Cells; D013997:Time Factors; D014162:Transfection; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D018076:DNA, Complementary; D005732:Gangliosides; D004247:DNA; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase