Distribution of tenascin-C and -X and expression of tenascin-C and X mRNA in the postnatal rat tongue.
Different distributions of tenascin-C and -X are found in various organs. However, the role of the tenascin family in the process of formation in the papillae epithelium during development is poorly understood. In order to find more information an tenascin-C and -X distributions during tongue development, immunohistocheminical studies have been carried out to demonstrate these distributions. The number of PCNA positive cells gradually increased from 5- to 15-days, and decreased on 21-days in the intercellular space of the epithelal layer in the postnatal development of rat tongue (150 specimens of Wistar male rats (0-, 5-, 10-, 15-, and 21-days). The reaction of tenascin-C was found mainly in the intercellular space of the epithelial layer on contrast to that of tenascin-X which was mainly found an the epithelial layer under a confocal laser scanning microscope. The level of mRNA of tenascin-C (600bp) and tenascin-X (588bp) gradually decreased from 5-days using RT-PCR methods. The different distribution of these extracellular matrices and weakly-regulated expressions may be related to the replication process of the epithelium in the tongue during development.
Watanabe
Kazuhiro
K
Department of Anatomy, School of Dentistry at Tokyo, The Nippon Dental University, Chiyoda-ku, Tokyo 102-8159, Japan.
Konishi
Kiyoshi
K
Sato
Iwao
I
D000375:Aging; D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009061:Mouth Mucosa / Q000254:growth & development / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D019063:Tenascin / Q000032:analysis / Q000235:genetics*; D014059:Tongue / Q000254:growth & development / Q000502:physiology*
D012333:RNA, Messenger; D019063:Tenascin; C081865:tenascin X