Maturation by LctT is required for biosynthesis of full-length lantibiotic lacticin 481.
In lantibiotic lacticin 481 biosynthesis, LctT cleaves the precursor peptide and exports mature lantibiotic. Matrix-assisted laser desorption ionization-time of flight mass spectrometry revealed that a truncated form of lacticin 481 is produced in the absence of LctT or after cleavage site inactivation. Production of truncated lacticin 481 is 4-fold less efficient, and its specific activity is about 10-fold lower.
Uguen
Patricia
P
Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne Sud, BP 92116, 56321 Lorient Cedex, France.
Hindré
Thomas
T
Didelot
Sandrine
S
Marty
Christel
C
Haras
Dominique
D
Le Pennec
Jean-Paul
JP
Vallée-Réhel
Karine
K
Dufour
Alain
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D001430:Bacteriocins; D004798:Enzymes; C481644:LctM protein, Lactococcus lactis; D011498:Protein Precursors; C082508:lacticin 481