Optimization of an analytical method for detecting paraphenylenediamine (PPD) by GC/MS-iontrap in biological liquids.
A method for qualitative and quantitative determination of paraphenylenediamine in biological fluids (blood, urine and gastric content) was developed. This analytical procedure involves deproteneisation or hydrolysis, liquid-liquid extraction, derivatization with TFA followed by GC-MS iontrap analysis. Benzidine was the internal standard used for quantification and the extraction recovery test was about 85%. The detection limit of paraphenylenediamine was determined at 0.1 pg (S/N=10).
Stambouli
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A
Laboratory of Forensic Sciences of Moroccan Gendarmerie Royale, BP. 6597, Rabat Instituts, CP 10100 Rabat-Maroc, Morocco. labgr@menara.ma
Bellimam
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MA
El Karni
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N
Bouayoun
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T
El Bouri
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A
D000293:Adolescent; D000328:Adult; D004396:Coloring Agents / Q000032:analysis* / Q000506:poisoning; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005766:Gastrointestinal Contents / Q000737:chemistry; D006801:Humans; D010655:Phenylenediamines / Q000032:analysis* / Q000506:poisoning; D011041:Poisoning / Q000175:diagnosis
D004396:Coloring Agents; D010655:Phenylenediamines; C029728:4-phenylenediamine