title: [A novel method of extracting total RNA from rice embryo samples].
abstract: A novel and simple CTAB-LiCl-based extraction method for high-quality and total RNA of rice embryo samples is developed. This method can efficiently eliminate the interference of polysaccharide and lipids rich in rice (Oryza sativa L.) embryo obtained under room temperature without using liquid nitrogen. The results of ultraviolet spectrophotometer and agarose gel electrophoresis analysis show that the obtained RNA has no obvious degradation and a good purity sufficient for further RT-PCR and RNA gel blotting. Therefore, it is also especially useful for the RNA extraction of plant material plenty of polysaccharide and lipids.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Rice Research Institute, Nanjing Agricultural University, Nanjing 210095, China. wanjm@njau.edu.cn
authors.forename: Wen-Biao
authors.initials: WB
authors.lastname: Shen
authors.forename: Ren
authors.initials: R
authors.lastname: Wang
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Tian-Qing
authors.initials: TQ
authors.lastname: Zheng
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Wan