title: Ship-in-a-bottle synthesis of fluorescence-labeled nanoparticles: applications in cellular imaging.
abstract: Fluorescein (2-(6-hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid) has been prepared inside the pores of zeolite-Y via ship-in-a-bottle synthesis. Fluorescein, whose dimensions prevent it from entering through the approximately 7 A windows of the faujasite zeolite used, was prepared by the acid-catalyzed reaction of resorcinol and phthalic anhydride. In this article we report initial spectroscopic data as well as an example of the usefulness of these fluorescence-labeled nanoparticles for imaging applications such as confocal fluorescence microscopy. Encapsulated fluorescein shows a remarkable increase in photostability.
authors.affiliation: Center for Catalysis Research and Innovation, Department of Chemistry, University of Ottawa, Ottawa, Canada.
authors.forename: Michelle N
authors.initials: MN
authors.lastname: Chrétien
authors.forename: Biao
authors.initials: B
authors.lastname: Shen
authors.forename: Hermenegildo
authors.initials: H
authors.lastname: García
authors.forename: Ann M
authors.initials: AM
authors.lastname: English
authors.forename: J C
authors.initials: JC
authors.lastname: Scaiano
mesh_terms: D002478:Cells, Cultured; D019793:Fluorescein / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017641:Zeolites / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D017641:Zeolites; D019793:Fluorescein