title: Microbial hydroxylation of cinobufagin by Mucor spinosus.
abstract: Mucor spinosus has been employed for the biotransformation of cinobufagin (1) to afford three metabolites. On the basis of their physico-chemical data, the structures of the transformation products have been characterized as 1beta-hydroxy-cinobufagin (2), 12beta-hydroxy-cinobufagin (3) and 1beta,12beta-dihydroxy-cinobufagin (4), of which metabolites 2 and 4 are new compounds. In vitro cytotoxic activities of the biotransformation products and the substrate-cinobufagin have been assayed against four tumor cell lines of Bel 7420, BGC 823, HeLa and HL 60; they all showed cytotoxic activities.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Gui-Qin
authors.initials: GQ
authors.lastname: Qu
authors.forename: Wan-Ying
authors.initials: WY
authors.lastname: Wu
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Chen
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D001711:Biotransformation; D002018:Bufanolides / Q000378:metabolism*; D006900:Hydroxylation; D009089:Mucor / Q000378:metabolism*
chemical_list: D002018:Bufanolides; C002471:cinobufagin