The effect of para-nonylphenol on Japanese eel (Anguilla japonica) spermatogenesis in vitro.
Endocrine disrupters have been recognized to interfere with endocrine systems that regulate reproduction, for example, by mimicking or inhibiting the action of endogenous sex steroid hormones including estradiol-17beta (E2). In the present study, we examined the effect of an endocrine disrupter, para-nonylphenol (p-NP) on spermatogenesis, and compared it with the action of E2, using an eel testicular organ culture system. p-NP alone stimulated early spermatogonial renewal in the same manner as E2. Neither induced further progress in spermatogenesis. In the presence of 11-ketotestosterone (11-KT), the major androgen in teleosts, p-NP did not prevent the 11-KT-induced progress in spermatogenesis. However, this treatment enlarged the Sertoli cells. Electron microscopic observation revealed that enlarged Sertoli cells contained well-developed organelles. Moreover, the proportion of germ cells appeared to have decreased as a result of Sertoli cell hypertrophy. These results clearly show that p-NP has an effect on Sertoli cells in the presence of an androgen (11-KT), potentially disturbing 11-KT-induced spermatogenesis.
Miura
C
C
Laboratory of Fish Reproductive Physiology, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
Takahashi
N
N
Michino
F
F
Miura
T
T
D000704:Analysis of Variance; D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001973:Bromodeoxyuridine; D004958:Estradiol / Q000633:toxicity; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D008854:Microscopy, Electron; D010636:Phenols / Q000633:toxicity*; D012708:Sertoli Cells / Q000187:drug effects / Q000648:ultrastructure; D013091:Spermatogenesis / Q000187:drug effects*; D013739:Testosterone / Q000031:analogs & derivatives*
D010636:Phenols; D013739:Testosterone; D004958:Estradiol; D001973:Bromodeoxyuridine; C041594:4-nonylphenol; C003600:11-ketotestosterone