[Polymorphism analysis of MHC-DRB3 gene in Dolang sheep with PCR-RFLP].
MHC is a chromosomal region consisting of a group of closely linked loci which are highly polymorphic, and plays a central role in the immune system. The restrictive polymorphism of MHC-DRB3 exon2 in Dolang sheep was Analyzed by PCR-RFLPs. The results revealed extensive polymorphisms 2, 2 and 6 RFLP types of PCR products were found with enzymes TaqI, PstI and HaeIII respectively. Considering all restrictive pattern, 24 alleles for DRB3 locus were found in Dolang sheep.
Liu
Yun-Fang
YF
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. lyf_6@Yahoo.com.cn
Yan
Gen-Qiang
GQ
Wang
Xin-Feng
XF
D000483:Alleles; D000818:Animals; D005091:Exons / Q000235:genetics; D008285:Major Histocompatibility Complex / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000235:genetics*