[Study on large-scale CD34+ cell enrichment using immunomagnetic isolation apparatus].
In order to find a method suitable for purifying large amount of CD34(+) cells, from 5 cases who accepted autologous peripheral blood stem cell transplantation, CD34(+) cells were collected and enriched by using Isolex 300i (Nexell). Phenotypes were detected by flow cytometry and the biological viability were assayed by the colony-forming experiments and cell expansion experiment in vitro. The results showed that the number of mononuclear cells first collected was about (3.5 - 6.0) x 10(10) and (0.55 - 1.2)% of cells were CD34 positive. The number of positive production was about (2.0 - 3.0) x 10(8); the CD34(+) cells purity was (75 - 85)% and the yield was (40 - 65)%. The CD34(+) cells of positive production could expand up to 2 - 3 times when cultured with SCF + IL3 + FL + TPO + EPO in vitro. The results of colony-forming experiments demonstrated that the CD34(+) cells collected has enough colony-forming ability. All results showed the enriched CD34(+) cells with biological viability. In conclusion, the CD34(+) immunomagnetic isolation apparatus Isolex300i is suitable to clinical application for a large amount of CD34(+) cell enrichment.
Liu
Hong
H
Department of Immunology, Affiliated Hospital of Oncology, Tianjing Medical University, Tianjing 300060, China. abcd-lh@eyou.com
Cao
Sui
S
Ren
Xiu-Bao
XB
Zhang
Peng
P
An
Xiu-Mei
XM
Hao
Xi-Shan
XS
D018952:Antigens, CD34 / Q000276:immunology*; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D009369:Neoplasms / Q000097:blood
D018952:Antigens, CD34