[Studies on expansion ex vivo of murine bone marrow cells and its hematopoietic reconstitution capacity].
OBJECTIVE
To investigate the effects of stem cell factor (SCF) in combination with interleukin-1 (IL-1) or/and interleukin-3 (IL-3) on ex vivo expansion of 5FU treated bone marrow cells and hematopoietic recovery in lethally irradiated mice transplanted with the expanded cells.


METHODS
5FU treated bone marrow cells (d3-5FU-BMC) were cultured in a cytokines-containing medium, and the net increments of CFU-GM and high proliferative potential colony forming cell (HPP-CFC) were evaluated.


RESULTS
CFU-GM increased by 33.7 +/- 18.1- or 18.1 +/- 6.3- fold, and HPP-CFC by 17.8 +/- 10.5- or 12.7 +/- 9.1- fold, respectively, in cultures containing SCF with IL- or IL-3, as compared with that in control; while SCF alone had little effect. Compared with fresh d3-5FU-BMC, transplantation of the expanded bone marrow cells accelerated the recovery of recipients' peripheral blood cell counts by 1 approximately 3 days and increased the survival rate of the transplanted animals (d3-5FU-BMC group 50% vs expansion group 8U approximately 100%).


CONCLUSIONS
SCF in combination with IL-1 or IL-3 synergetically ex vivo expands hematopoietic cells. Transplantation of the expanded bone marrow cells accelerates the recipient's hematopoietic reconstitution.
Han
M
M
Wan
S
S
Qian
L
L
Han
J
J
Li
X
X
Zhao
Y
Y
Peng
Q
Q
He
Y
Y
Bian
S
S
Yan
W
W
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D016026:Bone Marrow Transplantation; D049109:Cell Proliferation; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015996:Survival Rate
D005472:Fluorouracil