GeneChip expression analysis of the VqsR regulon of Pseudomonas aeruginosa TB.
Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type and VqsR mutant in ABC minimal medium. The expression of a large group of metabolic genes, ECF sigma factors as well as of many quorum-sensing genes previously not assigned to VqsR-regulon was found to be affected by the disruption of vqsR.
Juhas
Mario
M
Clinical Research Group OE 6711, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. juhas.mario@mh-hannover.de
Wiehlmann
Lutz
L
Salunkhe
Prabhakar
P
Lauber
Jörg
J
Buer
Jan
J
Tümmler
Burkhard
B
D003470:Culture Media; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000502:physiology; D018085:Regulon / Q000235:genetics; D012808:Sigma Factor / Q000502:physiology; D014774:Virulence / Q000235:genetics
D003470:Culture Media; D012808:Sigma Factor