In silico analysis of nonribosomal peptide synthetases of Xanthomonas axonopodis pv. citri: identification of putative siderophore and lipopeptide biosynthetic genes.
The genomes of the plant pathogens Xanthomonas axonopodis (Xac) and Xanthomonas campestris (Xcc) were analysed with the aim of deducing their ability to produce nonribosomal peptides. Nonribosomal peptide synthetase (NRPS) genes were identified in two separate loci of Xac. While the genes of locus 1 are common to both strains, locus 2 was only found in Xac. Dissection and phylogenetic analysis of the condensation and thioesterase domains of the NRPSs of loci 1 and 2 of Xac revealed homology, respectively, with siderophore and lipopeptide synthetases. Further analysis of locus 1 revealed genes related to polyketide and polyamine biosynthesis that could be involved in the assembly of substrates for siderophore biosynthesis in both strains. In vitro production of siderophores by both Xac and Xcc was confirmed. Since bacterial siderophores and lipopeptides can be pathogenic and are typically produced nonribosomally, these results suggest that the identified genes could be involved in phytotoxin production.
Etchegaray
Augusto
A
CENA, Universidade de São Paulo, 13400-970 Piracicaba, SP, Brazil. augusto.etchegaray@puc-campinas.edu.br
Silva-Stenico
Maria E
ME
Moon
David H
DH
Tsai
Siu M
SM
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D007501:Iron / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis* / Q000235:genetics; D018942:Macrolides / Q000378:metabolism; D015394:Molecular Structure; D046908:Peptide Biosynthesis, Nucleic Acid-Independent; D010453:Peptide Synthases / Q000235:genetics*; D010802:Phylogeny; D011073:Polyamines / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017385:Sequence Homology; D017262:Siderophores / Q000096:biosynthesis* / Q000235:genetics; D014974:Xanthomonas / Q000201:enzymology / Q000235:genetics*
D001426:Bacterial Proteins; D008074:Lipoproteins; D018942:Macrolides; D011073:Polyamines; D017262:Siderophores; D007501:Iron; D010453:Peptide Synthases