title: Triblock copolymers: synthesis, characterization, and delivery of a model protein.
abstract: The purpose of this study was to synthesize and characterize biodegradable and thermosensitive triblock copolymers for delivering protein at controlled rate in biologically active form for longer duration of time. A series of thermosensitive triblock copolymers with different block lengths (PLGA-PEG-PLGA) were synthesized by ring-opening polymerization of d,l-lactide and glycolide with polyethylene glycol (PEG) in the presence of stannous octoate. Compositions and molecular weight of triblock copolymers were characterized by 1H NMR spectrometry and gel permeation chromatography, respectively. A single test-tube inverting method was employed to determine the sol-gel transition temperature. Lysozyme was used as a model protein. Lysozyme solution formulation was prepared with different triblock copolymers for in vitro release. Lysozyme concentration and its biological activity in the released sample were determined using a standard MicroBCA method and bacterial cell lysis method, respectively. The effects of varying block lengths and concentrations of copolymers on the in vitro release of lysozyme were evaluated. The release profiles from formulations showed a higher initial release followed by slower release up to 4 weeks. Increasing the block lengths of copolymers decreased burst release of lysozyme from 41.2+/-5.4% to 16.1+/-3.9%. Increasing copolymer concentrations decreased the drug release. Lysozyme in the 4 weeks released samples retained most of its biological activity (>80%). It is feasible to deliver protein in biologically active form for longer duration by varying block lengths and concentrations of triblock copolymers.
authors.affiliation: Department of Pharmaceutical Sciences, North Dakota State University, PO Box 5055, Fargo, ND 58105, USA.
authors.forename: Sibao
authors.initials: S
authors.lastname: Chen
authors.forename: Robert
authors.initials: R
authors.lastname: Pieper
authors.forename: Dean C
authors.initials: DC
authors.lastname: Webster
authors.forename: Jagdish
authors.initials: J
authors.lastname: Singh
mesh_terms: D000818:Animals; D002645:Chickens; D016503:Drug Delivery Systems / Q000379:methods*; D016982:Micrococcus luteus / Q000187:drug effects / Q000201:enzymology; D009113:Muramidase / Q000008:administration & dosage / Q000493:pharmacokinetics; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D011098:Polyglactin 910 / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D011506:Proteins / Q000008:administration & dosage* / Q000493:pharmacokinetics
chemical_list: D011506:Proteins; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D011098:Polyglactin 910; D011092:Polyethylene Glycols; D009113:Muramidase