title: Synthesis and biological activity profile of new analogues of the cyclic opioid peptide H-Tyr-c[D-Cys-Gly-Phe(pNO2)-D-Cys]-NH2 containing (S)-alpha-hydroxymethylcysteine in place of cysteine.
abstract: To examine the effect on biological activity of replacing D-Cys in the opioid peptide H-Tyr-c[D-Cys-Gly-Phe(pNO(2))-D-Cys]-NH(2) in position 2 or/and 5 with alpha-hydroxymethylcysteine (alpha-Hmc), three analogues were synthesized. These compounds exhibit agonist activity at both mu and delta receptors. However, the most active analogue, with (S)-alpha-Hmc residue in position 5, was 3360- and 2190-fold less active than the parent peptide in the GPI and MVD assays, respectively.
authors.affiliation: Institute of Organic Chemistry, Technical University of Łódź, Łódź, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Witkowska
authors.forename: Nga N
authors.initials: NN
authors.lastname: Chung
authors.forename: Peter W
authors.initials: PW
authors.lastname: Schiller
authors.forename: Janusz
authors.initials: J
authors.lastname: Zabrocki
mesh_terms: D000595:Amino Acid Sequence; D003545:Cysteine / Q000031:analogs & derivatives*; D008970:Molecular Weight; D018847:Opioid Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D017465:Receptors, Opioid, delta / Q000819:agonists; D017450:Receptors, Opioid, mu / Q000819:agonists; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013329:Structure-Activity Relationship
chemical_list: D018847:Opioid Peptides; D010456:Peptides, Cyclic; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D003545:Cysteine