[The strategies of gene prolong expression in mammals].
Gene therapy holds great promises to a variety of inherited diseases. However, the limitations on extended and consistent foreign gene expression has severely hampered the development of applicable gene therapy approaches. Technologies are reviewed here including transponson integration, biolistic measures that pulse the naked plasmid into living organs, or the integration of eukaryotic cis elements into introns, 3' untranslated regions, or the integration of the EBV sequences, which could assist in the prolonged gene expression of the introduced foreign genes. These strategies may significantly promote the progresses of gene therapy.
Wang
Zong-Gui
ZG
Institute of Molecular Oncology, Guangzhou Liuhuaqiao Hospital, Guangzhou 510 010, China. wzgui825@sina.com
Zheng
Wen-Ling
WL
Cui
Dong
D
Ma
Wen-Li
WL
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics; D015870:Gene Expression / Q000502:physiology*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007438:Introns; D008322:Mammals; D010957:Plasmids
D004251:DNA Transposable Elements