Identification and characterization of a cell-wall anchored DNase gene in Clostridium perfringens.
Completion of the whole genome sequence of Clostridium perfringens strain 13 revealed the presence of an extracellular nuclease gene, cadA. Transcriptional analysis showed that the cadA gene is negatively regulated by the two-component VirR/VirS system and its secondary regulator VR-RNA. The CadA protein possesses an N-terminal signal sequence and a Gram-positive cell wall anchoring motif consisting of a sorting signal (LPXTG motif), a hydrophobic domain, and positively charged residues at the end of C-terminus. By comparing the DNase production between the wild type and the cadA mutant, and DNase activity assay with the recombinant truncated CadA protein, we confirmed that the cadA gene product is one of the DNases produced by C. perfringens.
Okumura
Kayo
K
Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1, Tennohdai, Tsukuba, Ibaraki 305-8575, Japan.
Kawsar
Hameem I
HI
Shimizu
Takeshi
T
Ohta
Toshiko
T
Hayashi
Hideo
H
Shimizu
Tohru
T
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology*; D003016:Clostridium perfringens / Q000201:enzymology* / Q000235:genetics; D003851:Deoxyribonucleases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D018085:Regulon / Q000502:physiology; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003851:Deoxyribonucleases