title: [Determination of isoimperatorin and osthol in ganmao yixiaoshi capsule by RP-HPLC].
abstract: OBJECTIVE
  To develop a method for the determination of isoimperatorin and osthol in Ganmao Yixiaoshi capsule by RP-HPLC.
  
  
  METHODS
  The chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of acetonitrile-water (42.5:57.5). The detection wavelength was at 320 nm.
  
  
  RESULTS
  The calibration curve was linear over the concentration range of 0-0.3975 microg for isoimperatorin and 0-0.3825 microg for osthol respectively. The correlation coefficient was r = 0.9999 and r = 0.9997. The average recovery of isoimperatorin was 98.1% and that of osthol was 99.8%.
  
  
  CONCLUSIONS
  The method sensitive, simple and accurate for the determination of isoimperatorin and osthol concentrations in Ganmao Yixiaoshi capsule.
authors.affiliation: School of Life Sciences and Engineerng, Tsinghua University, Beijing 100084, China. shiyue1029@hotmail.com
authors.forename: Yue
authors.initials: Y
authors.lastname: Shi
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Wang
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Du
mesh_terms: D029969:Angelica / Q000737:chemistry*; D019661:Apiaceae / Q000737:chemistry*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003139:Common Cold; D003374:Coumarins / Q000032:analysis*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D011564:Furocoumarins / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control
chemical_list: D002214:Capsules; D003374:Coumarins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C055542:isoimperatorin; C046627:osthol