title: Influence of naringenin on the activity of enzymes participating in steroidogenesis in male rats.
abstract: The aim of the experiment was to histochemically examine the activity of the following selected steroidogenic enzymes: delta5 3betaHSD and 17betaHSD and the housekeeping enzyme G6PDH, in experimental rats after subcutaneous injections of naringenin flavonoid, at a daily dose of 15 mg/kg of body mass. The enzyme activities were measured by the microdensitometric method. Additionally, radioimmunological assay for testosterone level was conducted in homogenates of testes. A significant decrease in the activity of 17betaHSD and of G6PDH was found, while the activity of delta5 3betaHSD was not significantly changed. The results permit a statement that naringenin causes minor changes in metabolic processes in the testes of rats but it does not significantly affect the synthesis of androgens.
authors.affiliation: Department of Cytobiology and Histochemistry, Pharmaceutical Faculty, Medical College, The Jagiellonian University, Kraków, Poland. mfpapiez@cyf-kr.edu.pl
authors.forename: M A
authors.initials: MA
authors.lastname: Papiez
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D004798:Enzymes / Q000378:metabolism*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D044950:Flavanones / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation; D013256:Steroids / Q000096:biosynthesis*
chemical_list: D004798:Enzymes; D004965:Estrogen Antagonists; D044950:Flavanones; D013256:Steroids; D015067:17-Hydroxysteroid Dehydrogenases; D005954:Glucosephosphate Dehydrogenase; C005273:naringenin