[Studies of somatic embryogenesis and genetic transformation by Agrobacterium- mediated in soybean].
Somatic embryogenesis was induced and the regenerated plants were obtained by higher concentrations of auxins with immature cotyledon of 55 genotypes in soybean. Bivalent insect resistant genes were transformed into immature cotyledon of soybean which have high frequency of somatic embryogenesis via Agrobacterium-mediated. The results showed that 14 genotypes possessed high frequency of somatic embryogenesis (more than 40%) among soybean genotypes from Northeast area. 2147 immature cotyledons of 5 different soybean genotypes cultured in Northeast area were inoculated with LBA4404 (including pGBI121S4ABC plasmid). 17 plantlets were obtained under Kanamicy selection. 12 plantlets showed positive reaction in PCTR and PCR-Southern detection. These analyses confirmed the presence of introduced Bt gene in soybean.
Wang
Ping
P
Heilongjiang Academy of Agricultural Sciences, Ministry of Science and Technology, Harbin 150 086, P.R. China. y-pwang@yahoo.com.cn
Wang
Gang
G
Ji
Jing
J
Zeng
Fang-Ting
FT
Huang
Bin-Cheng
BC
Cao
Jiang
J
Wu
Ying
Y
D018548:Cotyledon / Q000196:embryology; D017343:Genes, Plant; D005838:Genotype; D030821:Plants, Genetically Modified / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology; D013025:Glycine max / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D014170:Transformation, Genetic