Differential regulation of metallothionein-I and metallothionein-II mRNA expression in the rat brain following traumatic brain injury.
In this study, we investigated the expression of metallothionein (MT)-I and MT-II in the rat brain following traumatic brain injury (TBI). In the early stage, significant induction of MT-I and MT-II were observed in various regions including ventricle walls, pia mater, and dentate gyrus. At 12-24 h after TBI, strong induction of MT-I mRNA was observed in cerebral cortical layer II/III, amygdala, and piriform cortex where neurons reside. On the other hand, MT-II appeared to be expressed mainly in glial cells localized in the cerebral cortex and hippocampal formation. Three days after TBI, MTs were observed in the vimentin-positive astrocytes in the penumbra as revealed by double immunohistochemistry. The differences in expression of MT-I and MT-II in different brain regions and cell types (neuron vs. glial cells) suggests that multiple regulatory mechanisms are involved in the control of MT expression following brain injury.
Kim
Eun Hae
EH
Department of Anatomy, College of Medicine, Brain Korea 21, Korea University, Seoul 136-705, Korea.
Kim
Tae Sik
TS
Sun
Woong
W
Kim
Dal Soo
DS
Chung
Hyun-Soo
HS
Kim
Dong Ki
DK
Park
Sun-Hwa
SH
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D016207:Cytokines; D012333:RNA, Messenger; D008668:Metallothionein