[Expression of HSP70 in human umbilical vein endothelial cells induced by Hantavirus].
OBJECTIVE
To study stress response in human umbilical vein endothelial cells (HUVEC) infected with Hantavirus (HTV).


METHODS
The expression of HSP70 was detected by immunocytochemical staining, in situ nucleic acid hybridization and RT-PCR.


RESULTS
After being infected with HTV, the expression of HSP70 in HUVEC increased. RT-PCR showed that the level of HSP70 mRNA increased rapidly and kept at a high level in comparison with the normal control (P<0.05).


CONCLUSIONS
The over-expression of HSP70 in HUVECs can be induced by Hantavirus infection, which may play a role in the inhibition of virus reproduction and the protection of cells from virus infection.
Yu
Lu
L
Department of Pathology ,Fourth Military Medical University, Xi'an 710032, China.
Ma
Heng
H
Yang
Shou-Jing
SJ
D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism* / Q000821:virology*; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000494:pharmacology; D006476:Orthohantavirus / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology*
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger