title: Differential expression of cdk inhibitors p16, p21cip1, p27kip1, and cyclin E in cervical cytological smears prepared by the ThinPrep method.
abstract: Our objective was to correlate p16, p21cip1, p27kip1, and cyclin E protein expression with the degree of dysplasia on ThinPrep Papanicolaou (Pap) smears using a modified immunoperoxidase staining. Smears read as normal, atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL), or high-grade SIL (HSIL) were identified and tested for high-risk human papillomavirus (HR-HPV). Additional smears were processed for immunoperoxidase for p16, p21cip1, p27kip1, and cyclin E. Thirty-four smears were satisfactory for study. The p16 was positive in all nine HSIL, in four of nine LSIL, and in one of seven ASC-US. The p27kip1 was positive in all nine HSIL, in eight of nine LSIL, and in one of seven ASC-US. The p21cip1 was positive in all nine HSIL, in one of nine LSIL, and in one of seven ASC-US. Cyclin E was positive in seven of nine HSIL and in one of nine LSIL and in none of the ASC-US smears. Normal smears were negative for all the antigens. There was poor correlation of protein expression and HR-HPV infection. We concluded that p16, p21cip1, p27kip1, and cyclin E can be demonstrated on Pap smears and they are expressed differentially in dysplastic cells, with highest expression in HSIL. The p21cip1 and cyclin E showed the greatest correlation with HSIL.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Louisville, Louisville, Kentucky 40292, USA. gmoor02@gwise.louisville.edu
authors.forename: Grace D
authors.initials: GD
authors.lastname: Moore
authors.forename: Sheron C
authors.initials: SC
authors.lastname: Lear
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Wills-Frank
authors.forename: Alvin W
authors.initials: AW
authors.lastname: Martin
authors.forename: James W
authors.initials: JW
authors.lastname: Snyder
authors.forename: C William
authors.initials: CW
authors.lastname: Helm
mesh_terms: D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D065006:Papanicolaou Test; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D018797:Cell Cycle Proteins