[Rapid construction of full-length MnSOD cDNA of chickens by one-step 3'RACE].
RACE (rapid amplification of cDNA ends) is a popular technique to rapidly obtain the full-length cDNA. After obtaining the 3' cDNA and 5' cDNA fragments with a overlapped region by 3' RACE and 5' RACE, the full-length cDNA could be generated by end-to-end PCR or subcloning. In this study, 3' RACE combined with touch-down PCR was successfully used for the rapid construction of full-length MnSOD cDNA of chickens. Compared with the conventional end-to-end PCR or subcloning, this method, called one-step 3' RACE, is fast, economical and highly specific. It especially fits the rapid construction of full-length cDNA by RACE method.
Bu
You-Quan
YQ
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100 094, China.
Luo
Xu-Gang
XG
Liu
Bin
B
Li
Su-Fen
SF
D000818:Animals; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D009206:Myocardium / Q000737:chemistry; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D013482:Superoxide Dismutase / Q000235:genetics*
D018076:DNA, Complementary; D013482:Superoxide Dismutase