title: Upregulation of neuronal nitric oxide synthase mRNA and protein in adrenal medulla of water-deprived rats.
abstract: Experiments were performed to investigate whether adrenal neuronal nitric oxide synthase (nNOS) mRNA and protein expression are responsive to alterations in body volume. Using an RT-PCR technique, the relative quantities of nNOS mRNA as well as the tyrosine hydroxylase and phenylethanolamine N-methyltransferase mRNA in the adrenals of water-deprived rats significantly increased from 12 hr to 4 days. In situ hybridization and immunohistochemical study showed that water deprivation activated nNOS mRNA and protein expression in the adrenal medulla. Four days after water deprivation, nNOS protein expression determined by Western blot significantly increased in the adrenal gland. Our results are the first to demonstrate that nNOS syntheses in the adrenal medulla are markedly increased in water-deprived rats. This study also indicates that the upregulation of nNOS synthesis of the adrenal medulla is associated with the activation of adrenal medullary function in the face of volume depletion.
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung , Taiwan.
authors.forename: Feng-Jie
authors.initials: FJ
authors.lastname: Lai
authors.forename: Shiue-Shin
authors.initials: SS
authors.lastname: Huang
authors.forename: Ming-Chia
authors.initials: MC
authors.lastname: Hsieh
authors.forename: Shih-Chie
authors.initials: SC
authors.lastname: Hsin
authors.forename: Chin-Han
authors.initials: CH
authors.lastname: Wu
authors.forename: Ya-Chieh
authors.initials: YC
authors.lastname: Hsin
authors.forename: Shyi-Jang
authors.initials: SJ
authors.lastname: Shin
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052248:Nitric Oxide Synthase Type I; D010625:Phenylethanolamine N-Methyltransferase / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation; D014868:Water Deprivation
chemical_list: D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase