Upregulation of neuronal nitric oxide synthase mRNA and protein in adrenal medulla of water-deprived rats.
Experiments were performed to investigate whether adrenal neuronal nitric oxide synthase (nNOS) mRNA and protein expression are responsive to alterations in body volume. Using an RT-PCR technique, the relative quantities of nNOS mRNA as well as the tyrosine hydroxylase and phenylethanolamine N-methyltransferase mRNA in the adrenals of water-deprived rats significantly increased from 12 hr to 4 days. In situ hybridization and immunohistochemical study showed that water deprivation activated nNOS mRNA and protein expression in the adrenal medulla. Four days after water deprivation, nNOS protein expression determined by Western blot significantly increased in the adrenal gland. Our results are the first to demonstrate that nNOS syntheses in the adrenal medulla are markedly increased in water-deprived rats. This study also indicates that the upregulation of nNOS synthesis of the adrenal medulla is associated with the activation of adrenal medullary function in the face of volume depletion.
Lai
Feng-Jie
FJ
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung , Taiwan.
Huang
Shiue-Shin
SS
Hsieh
Ming-Chia
MC
Hsin
Shih-Chie
SC
Wu
Chin-Han
CH
Hsin
Ya-Chieh
YC
Shin
Shyi-Jang
SJ
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052248:Nitric Oxide Synthase Type I; D010625:Phenylethanolamine N-Methyltransferase / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation; D014868:Water Deprivation
D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase