[A direct immunogold-silver staining method for diagnosing hemorrhagic fever with renal syndrome].
A direct immunogold-silver staining (D-IGSS) method with 5nm colloidal gold-labeled rabbit anti-HFRS IgG antibodies was employed to detect HFRS virus antigen in the cytoplasm of white blood cells from HFRS patients and compared with indirect immunofluorescence assay (I-IFA). The results showed as follows: 1. The positive rate (87.5%) of HFRSV antigen detected by D-IGSS was significantly higher than that (65.6%) detected by I-IFA. in the white blood cells (P<0.05); 2. With the D-IGSS method the positive rates of HFRSV antigen in white blood cells of finger and vein blood were 81.3% and 87.5% respectively (P>0.05) with no significant difference but finger blood smears is more simple. We belive the D-IGSS method can be widely used for early diagnosis of HFRS at basic-level hospitals.
Luo
D
D
Union Hospital, Tongji Medical University, Wuhan 430022.
Wang
Y
Y
Zeng
L
L
Zhang
S
S
D000818:Animals; D002522:Chlorocebus aethiops; D006480:Hemorrhagic Fever with Renal Syndrome / Q000175:diagnosis* / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D016622:Silver Staining / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D014709:Vero Cells