title: Measurement of isoflavones using liquid chromatography with multi-channel coulometric electrochemical detection.
abstract: We evaluated the use of HPLC multi-channel coulometric electrochemical detection (HPLC-ECD) for the routine measurement of phytochemicals by analyzing standards and urine samples. Compounds were separated chromatographically and electrochemically with high sensitivity (10 ng/ml of isoflavones in urine). HPLC-ECD will enable better investigation into the relationship between lifestyle and the effects of phytochemicals on humans.
authors.affiliation: Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-0054, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ouchi
authors.forename: Paul
authors.initials: P
authors.lastname: Gamache
authors.forename: Ian
authors.initials: I
authors.lastname: Acworth
authors.forename: Shaw
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D060754:Equol; D019833:Genistein / Q000032:analysis*; D007202:Indicators and Reagents; D007529:Isoflavones / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
chemical_list: C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D007202:Indicators and Reagents; D007529:Isoflavones; D060754:Equol; C004742:daidzein; D019833:Genistein