Effects of retinoic acid on the beta-catenin/TCF pathway in cultured porcine tracheobronchial epithelial cells.
The effects of retinoic acid on the beta-catenin/TCF pathway in cultured porcine tracheobronchial epithelial cells (TBEC) were investigated. After TBEC were treated with retinoic acid at various concentrations, mRNA and protein changes of beta-catenin in cytoplasm, nucleus and whole cell of the TBEC were observed by immunocytochemical stain, RT-PCR and Western blotting. And the changes of the target gene cyclinD1 of beta-catenin/TCF pathway were also observed. It was found that there was no significant difference in beta-cat mRNA level after retinoic acid treatment. However, the expression of beta-catenin in the whole cell and cytoplasm was elevated with the increase of retinoic acid concentration (P<0. 01). The nuclear protein beta-catenin and target gene cyclinD1 of beta-catenin/TCF pathway was decreased (P<0.05). It was indicated that retinoic acid could increase beta-catenin level of the whole cell protein and decrease nuclear beta-catenin, downregulating beta-cat/TCF signaling activity and reducing target gene cyclinD1 protein level. As a result, retinoic acid can downregulate beta-catenin/TCF pathway in porcine tracheobronchial epithelial cell, suggesting that retinoic acid can inhibit the proliferation and accelerate differentiation of tracheobronchial epithelial cells.
Li
Yuan
Y
Department of Pathology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wu
Renliang
R
Wang
Xi
X
Chen
Wenshu
W
D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction; D013552:Swine; D051820:TCF Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014132:Trachea / Q000166:cytology / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D051176:beta Catenin / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D051820:TCF Transcription Factors; D051176:beta Catenin; D019938:Cyclin D1; D014212:Tretinoin