Presence of MHC-I in rat glioma cells expressing antisense IGF-I-receptor RNA.
The supposed immunogenic character of glioma cells transfected with antisense IGF-I-Receptor (IGF-I-R) expression vector was tested for the presence of MHC-I currently present in cells of IGF-I antisense type. C6 rat glioma cell line was comparatively transfected in vitro with IGF I antisense (pMT-Anti-IGF I) or IGF I Receptor antisense (pMT-Anti-IGF I R) expression vectors. The wild and transfected cells were examined for the presence of IGF-I and MHC-I molecules. Using RT PCR technique, the transfected "antisens" cells showed total inhibition of IGF-I. The both transfected cultures of IGF-I and of IGF-I-R type were positively stained for MHC-I. Moreover "antisense IGF-I-R" cells as compared to "IGF-I antisense" cells showed slightly higher expression of MHC-I. The transfected cells showed also the feature of apoptosis in 60% of cells. The immunogenicity of IGF-I-R antisense glioma cells is related to MHC-I presence; therefore both approaches of antisense IGF-I and of antisense IGF-I-R could be use in paralel for cellular therapy of glioblastoma.
Szpechcinski
A
A
Medical University and University Hospital, Bydgoszcz, Poland.
Trzos
R
R
Jarocki
P
P
Trojan
L A
LA
Oficjalska
K
K
Junkiert
A
A
Wei
M X
MX
Mazurek
M
M
Czapiewska
J L
JL
Niklinski
J
J
Kopiński
P
P
Chyczewski
L
L
Kasacka
I
I
Trojan
J
J
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D017931:DNA Primers; D005910:Glioma / Q000235:genetics* / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D016372:RNA, Antisense / Q000235:genetics*; D051381:Rats; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D016372:RNA, Antisense; D017526:Receptor, IGF Type 1