title: Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.
abstract: RNA polymerase II (pol II) purified from the fission yeast Schizosaccharomyces pombe was previously reported to be associated with the general transcription factor TFIIF and the C-terminal domain phosphatase Fcp1, as well as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which has recently been implicated in transcriptional activation in human cells. Here, we provide evidence that the Rpb7 subunit of pol II interacts with GAPDH. Two-hybrid screen identified GAPDH as an Rpb7-binding protein. In addition, GAPDH was affinity-purified from S. pombe extract by using an Rpb4/Rpb7-coupled column. We also identified actin as a pol II-associated protein and revealed the interaction between actin and Rpb7.
authors.affiliation: Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. hmitsuza@lab.nig.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitsuzawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.forename: Emi
authors.initials: E
authors.lastname: Kanda
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
mesh_terms: D000199:Actins / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D000199:Actins; D029702:Schizosaccharomyces pombe Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D012319:RNA Polymerase II; C496332:Rpb7 protein, S pombe