[The ALP activity of cultured dental coronal and root pulp cells in vitro].
OBJECTIVE
To compare the ALP activity in human dental coronal and root pulp,and investigate the localization of dental pulp stem cells in the dental pulp.


METHODS
The human dental root and coronal pulp cells were cultured in tissue-explant method, the 5th generation of cells in good growth condition were subcultured with conditioned media (20% DMEM + 10 nmol/L + dexamethaone + 50mg/L ascorbic acid) to induce mineralization. At the days of 5, 10, 15 after inducing, Gomori alkaline phosphatase stain was used to investigate alkaline phosphatase (ALP) activity in root and coronal pulp cells.


RESULTS
Positive staining was found in both root and coronal cells, the ALP activity was equal at 5 days of incubation, but higher in root pulp cells than that of coronal pulp cells after 10 and 15 days of incubation.


CONCLUSIONS
ALP activity in dental root pulp is higher than that in coronal pulp. DPSCs may exist in both dental root and coronal pulp, and the density of DPSCs is higher in the root pulp than the coronal pulp.
Sun
Shan-zhen
SZ
Dental School of Shangdong University, Jinan 250012, Shandong Province, China. sshzh@sdu.edu.cn
Liu
Shao-hua
SH
Wei
Feng-cai
FC
Zhang
Chun-yan
CY
Liu
Yun-sheng
YS
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques
D000469:Alkaline Phosphatase