title: Evaluation of five elements in lenses and aqueous humour of experimental rabbits after induced opacity.
abstract: We evaluated the presence of Ca, Na, K, Cu and Zn in the lenses and aqueous humour of rabbits treated with an Nd:YAG laser to induce opacity of the crystalline. The mean concentrations of the elements found in control lenses were: Ca: 15.8+/-5.2 mg/kg; Na: 1.2+/-0.6 g/kg; K: 10.3+/-3.3 g/kg; Cu: 0.19+/-0.06 mg/kg; Zn: 20.6+/-3.0 mg/kg. With the exception of K and Zn, the values found in the lenses of treated eyes (Ca: 135+/-24 mg/kg; Na: 4.3+/-1.5 g/kg; K: 10.1 +/- 3.2 g/kg; Cu: 0.47+/-0.17 mg/kg; Zn: 21.8+/-4.2 mg/kg) were significantly higher than in the controls. On the other hand, the concentrations found in the aqueous humour of treated eyes (Ca: 21.7+/-4.5 mg/l; Na: 0.66+/-0.21 g/l; K: 0.29+/-0.10 g/l; Cu: 0.035+/-0.009 mg/l; Zn: 0.079+/-0.01 mg/l) were significantly lower than those of the controls. The greatest difference was observed for Na (-68.6%) and Cu (-52.7%), followed by Ca and Zn (-35.0% and -35.2%, respectively). A positive correlation was found between Ca and Na in treated lenses (r2 = 0.9226, p < 0.0001) whereas inverse correlations were found for both Ca (r2 = 0.9788, p<0.0001) and Na (r2 = 0.9491, p<0.0001) between the concentrations found in the lenses and in the aqueous humour of treated eyes.
authors.affiliation: II Div. Oculistica, Institute of Ophthalmology, University La Sapienza, Viale del Policlinico, 1, Rome, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Cruciani
authors.forename: Antonietta
authors.initials: A
authors.lastname: Moramarco
authors.forename: Barbara
authors.initials: B
authors.lastname: Antonelli
authors.forename: Roberto
authors.initials: R
authors.lastname: Mollo
authors.forename: Corrado
authors.initials: C
authors.lastname: Balacco-Gabrieli
authors.forename: Sergio
authors.initials: S
authors.lastname: Costantini
authors.forename: Laura
authors.initials: L
authors.lastname: Ciaralli
authors.forename: Rosa
authors.initials: R
authors.lastname: Giordano
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sepe
authors.forename: Emanuela
authors.initials: E
authors.lastname: Damore
authors.forename: Mauro
authors.initials: M
authors.lastname: Valeri
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D002118:Calcium / Q000032:analysis; D002386:Cataract / Q000209:etiology / Q000378:metabolism*; D002412:Cations / Q000032:analysis*; D003300:Copper / Q000032:analysis; D004195:Disease Models, Animal; D007834:Lasers; D007908:Lens, Crystalline / Q000737:chemistry*; D008297:Male; D011188:Potassium / Q000032:analysis; D011817:Rabbits; D012964:Sodium / Q000032:analysis; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000032:analysis
chemical_list: D002412:Cations; D003300:Copper; D012964:Sodium; D015032:Zinc; D011188:Potassium; D002118:Calcium