title: Electron paramagnetic resonance and fluorescence in situ hybridization-based investigations of individual doses for persons living at Metlino in the upper reaches of the Techa River.
abstract: Waterborne releases to the Techa River from the Mayak Production Association in Russia during 1949-1956 resulted in significant doses to persons living downstream; the most contaminated village was Metlino, about 7 km from the site of release. Internal and external doses have been estimated for these residents using the Techa River Dosimetry System-2000 (TRDS-2000); the primary purpose is to support epidemiological studies of the members of the Extended Techa River Cohort. Efforts to validate the calculations of external and internal dose are considered essential. One validation study of the TRDS-2000 system has been performed by the comparison of calculated doses to quartz from bricks in old buildings at Metlino with those measured by luminescence dosimetry. Two additional methods of validation considered here are electron paramagnetic resonance (EPR) measurements of teeth and fluorescence in situ hybridization (FISH) measurements of chromosome translocations in circulating lymphocytes. For electron paramagnetic resonance, 36 measurements on 26 teeth from 16 donors from Metlino were made at the GSF-National Research Center for Environment and Health (16 measurements) and the Institute of Metal Physics (20 measurements); the correlation among measurements made at the two laboratories has been found to be 0.99. Background measurements were also made on 218 teeth (63 molars, 128 premolars, and 27 incisors). Fluorescence in situ hybridization measurements were made for 31 residents of Metlino. These measurements were handicapped by the analysis of a limited number of cells; for several individuals no stable translocations were observed. Fluorescence in situ hybridization measurements were also made for 39 individuals believed to be unexposed. The EPR- and FISH-based estimates agreed well for permanent residents of Metlino: 0.67 +/- 0.21 Gy and 0.48 +/- 0.18 Gy (mean +/- standard error of the mean), respectively. Results of the two experimental methods also agreed well with the estimates derived from the use of the TRDS-2000. For all persons investigated according to each technique, the EPR-measured dose to enamel was 0.55 +/- 0.17 Gy, and the TRDS-2000 prediction for the dose to enamel for these individuals is 0.55 +/- 0.07 Gy. The fluorescence in situ hybridization-based dose, 0.38 +/- 0.10 Gy, compared well to the TRDS-2000 prediction of external dose, 0.31 +/- 0.03 Gy, to red bone marrow for these persons. Validation of external doses at the remaining villages is an active area of investigation.
authors.affiliation: Urals Research Center for Radiation Medicine, 454076 Chelyabinsk, Russia.
authors.forename: Marina O
authors.initials: MO
authors.lastname: Degteva
authors.forename: Lynn R
authors.initials: LR
authors.lastname: Anspaugh
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Akleyev
authors.forename: Peter
authors.initials: P
authors.lastname: Jacob
authors.forename: Denis V
authors.initials: DV
authors.lastname: Ivanov
authors.forename: Albrecht
authors.initials: A
authors.lastname: Wieser
authors.forename: Marina I
authors.initials: MI
authors.lastname: Vorobiova
authors.forename: Elena A
authors.initials: EA
authors.lastname: Shishkina
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Shved
authors.forename: Alexandra
authors.initials: A
authors.lastname: Vozilova
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Bayankin
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Napier
mesh_terms: D000368:Aged; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020223:Chromosome Painting / Q000379:methods; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D008875:Middle Aged; D011829:Radiation Dosage; D011834:Radiation Monitoring; D011850:Radioactive Waste; D014070:Tooth / Q000528:radiation effects* / Q000648:ultrastructure; D014875:Water Pollutants, Radioactive
chemical_list: D011850:Radioactive Waste; D014875:Water Pollutants, Radioactive