First proteomic analysis of Legionella pneumophila based on its developing genome sequence.
The first proteomic analysis of the respiratory pathogen Legionella pneumophila ATCC 33152 is presented in this report. Two-dimensional gel electrophoresis of total cell extracts was carried out. In total, 130 protein spots were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MS) or by quadruple time-of-flight tandem MS, including proteins correlated with virulence. For the first time, proteins of L. pneumophila were identified using mass spectrometric methods and mapped on a two-dimensional gel; this will be of considerable use for comparison of protein expression profiles of L. pneumophila wild-type and knock-out mutant strains and of L. pneumophila grown under different conditions.
Lebeau
Ilya
I
Laboratory of Bacteriology, Rega Instituut, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium.
Lammertyn
Elke
E
De Buck
Emmy
E
Maes
Liesbeth
L
Geukens
Nick
N
Van Mellaert
Lieve
L
Arckens
Lut
L
Anné
Jozef
J
Clerens
Stefan
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D016952:Legionella pneumophila / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D040901:Proteomics; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D020543:Proteome