Genotyping of Toxoplasma gondii strains isolated from dogs with neurological signs.
From 111 dogs with neurological signs admitted in this research in a 22-month period, brain samples of 34 animals were inoculated in mice in order to isolate Toxoplasma gondii. From these 34 dogs, 9 strains of T. gondii were isolated and the genetic characterization performed by restriction analysis (RFLP) of the SAG-2 gene. RFLP analysis showed that four of them were classified as Type I, and five as Type III. The present report is the first description of genotyping of T. gondii isolated from brain samples of naturally infected dogs, in Brazil.
da Silva
Aristeu Vieira
AV
Universidade Paranaense (UNIPAR), Umuarama, Paraná, Brazil. silva.av@uol.com.br
Pezerico
Sandia Bergamaschi
SB
de Lima
Vanessa Yuri
VY
d'Arc Moretti
Leandro
L
Pinheiro
Julia Plombom
JP
Tanaka
Erica Maeme
EM
Ribeiro
Márcio Garcia
MG
Langoni
Helio
H
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics; D001681:Biological Assay / Q000662:veterinary; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology* / Q000473:pathology; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005838:Genotype; D051379:Mice; D009422:Nervous System Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology* / Q000473:pathology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C067547:surface antigen P22, Toxoplasma