Evaluation of proliferating markers Ki-67, PCNA in gastric cancers.
Tumours from 45 patients with advanced gastric cancer were assessed by immunohistochemistry. Tissue sections were fixed in 10% buffered formaldehyde solution, embedded in paraffin and stained immunohistochemically with anti-human Ki-67 and PCNA antibodies. No correlation was found between Ki-67, PCNA protein expression, the age of patients and the localization of tumour. A significant, positive association was observed between the expression of Ki-67, PCNA and tumour differentiation and Lauren's classification. Also a strong correlation was found between lymph node involvement and the expression of Ki-67 protein. These data suggest that the expression of Ki-67, PCNA proteins were closely connected with the high grade of tumour malignancy.
Czyzewska
J
J
Department of Gastroenterology and Internal Disease, Medical University of Białystok, Poland.
Guzińska-Ustymowicz
K
K
Lebelt
A
A
Zalewski
B
B
Kemona
A
A
D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D009367:Neoplasm Staging; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D013274:Stomach Neoplasms / Q000473:pathology* / Q000601:surgery
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen