title: Amino acid sequence of alpha-subunit in hen egg white ovomucin deduced from cloned cDNA.
abstract: The primary amino acid sequence of alpha-subunit in ovomucin (OVM) from hen thick egg white was determined. The 2087 amino acid residues with a relative molecular mass of 230.9 kDa along the full length of the alpha-subunit were represented. The alpha-subunit contains domains, arranged from the N- to C-terminals in the following order: D1-D2-D'-D3-R (central region)-D4-C1-CK (Cystine-knot), in a manner similar to the arrangement of D, C and CK domains in human pre-pro-von Willebrand factor (hpp-vWF) and hMUC2. The alpha-subunit showed identities on amino acid sequences with hpp-vWF and hMUC2 at 33 and 41% in the N-terminal region and 30 and 38% in the C-terminal region, respectively. The numbers and positions of cysteine residues were highly conserved among alpha-subunit, hpp-vWF and hMUC2. However, R showed no virtual sequence homology with the corresponding regions in two proteins. It was estimated that alpha-subunit was not part of a large peptide of OVM, but was independently synthesized from beta-subunit.
authors.affiliation: Faculty of Agriculture, Tokyo University of Agriculture, Funako 1737, Atsugishi, Kanagawa 243-0034, Japan. watak@nodai.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimoyamada
authors.forename: Takuo
authors.initials: T
authors.lastname: Onizuka
authors.forename: Hideo
authors.initials: H
authors.lastname: Akiyama
authors.forename: Motohiro
authors.initials: M
authors.lastname: Niwa
authors.forename: Tomoe
authors.initials: T
authors.lastname: Ido
authors.forename: Yoji
authors.initials: Y
authors.lastname: Tsuge
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens / Q000235:genetics; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010059:Ovomucin / Q000235:genetics*; D020539:Sequence Analysis, Protein
chemical_list: D018076:DNA, Complementary; D010059:Ovomucin