The energy profiling of short interfering RNAs is highly predictive of their activity.
The technology of knockdown of gene expression by synthetic short interfering RNAs (siRNAs) is currently diffused because of its relative simplicity and reliability. Despite the claims that active siRNAs can be easily identified, how to select active siRNAs with high efficiency it is not fully defined. Here, we report that energy profiling can be used in the guidelines for siRNA design.
Poliseno
Laura
L
Laboratorio di Terapia Genica e Molecolare, Istituto di Fisiologia Clinica, CNR, Pisa, Italy.
Evangelista
Monica
M
Mercatanti
Alberto
A
Mariani
Laura
L
Citti
Lorenzo
L
Rainaldi
Giuseppe
G
D000818:Animals; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics; D013552:Swine; D013816:Thermodynamics
D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins