[Inactivation of T4 phage in water environment using proteinase].
The inactivation effectiveness of proteinase to viruses was investigated by using T4 phage as a model virus. The results showed that the inactivation effectiveness of proteinase to T4 phage was obvious. In the optimum conditions and 67.5 u/mL concentration, the inactivation rate of proteinase K to T4 phage in sterilized water and in sewage achieved 99.4% and 49.4% respectively in an hour, and achieved >99.9% and 81.1% in three hours. The inactivation rate of the industrial proteinase 1398 to T4 phage in sterilized water achieved 74.4% in an hour. The effects of pH and temperature on the inactivation effectiveness was not evident.
Lü
Wen-zhou
WZ
SKLEAC, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Yang
Qing-xiang
QX
Zhang
Yu
Y
Yang
Min
M
Zhu
Chun-fang
CF
D017122:Bacteriophage T4 / Q000187:drug effects* / Q000302:isolation & purification; D019286:Endopeptidase K / Q000494:pharmacology*; D005618:Fresh Water / Q000382:microbiology*; D014871:Water Microbiology
D019286:Endopeptidase K