[9-cis retinoic acid induces apoptosis in HL-60 cells].
OBJECTIVE
To investigate the apoptosis of HL-60 cells induced by 9-cis retinoic acid (9-cis RA), and illustrate the possible molecular mechanism.


METHODS
Apoptosis was detected by morphological observation, DNA electrophoresis and flow cytometric cell cycle analysis. Bcl-2 expression was detected by flow cytometry.


RESULTS
9-cis RA initiated apoptosis of HL-60 cells after inducing them irreversibly commited to differentiation. Bcl-2 was downregulated during the differentiation and apoptosis process. 9-cis RA was more potent than all-trans retinoic acid (ATRA) did in inducing terminal differentiation associated apoptosis and in downregulation of Bcl-2 expression.


CONCLUSIONS
9-cis RA can induce apoptosis in HL-60 cells. Downregulation of Bcl-2 expression appears to play an important role in the apoptosis of the differentiated leukemic cells.
Hui
Z
Z
Department of Hematology, Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing.
Zhao
X
X
Ma
Y
Y
Ai
E
E
Zhao
R
R
Liu
F
F
Yang
M
M
Ju
S
S
Wang
J
J
Gao
T
T
D000077556:Alitretinoin; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*
D019253:Proto-Oncogene Proteins c-bcl-2; D000077556:Alitretinoin; D014212:Tretinoin