[Study on red cell enzymes and isoenzymes in patients with leukemia and myelodysplastic syndromes].
OBJECTIVE
To define the prevalence of acquired red cell enzymopathy in leukemia and MDS patients and explore its clinical significance.


METHODS
Red cell enzymes (G6PD, 6PGD, PK, ENOL, ADA, PNP,ALD) activities and PK,ADA isoenzymes were assayed by the methods recommended by ICSH and PAGE electrophoresis.


RESULTS
The prevalence rate of G6PD deficiency was 43.1%, and of PK deficiency was 27.8%, in leukemia and MDS patients. The prevalence rates of PK, G6PD and PNP deficiency in MDS patients were 50%, 48% and 39.1%, respectively, while of ADA, ENOL and ALD over production in MDS patients were 69. 6%, 40% and 30. 4%, respectively. PK activity was increased in CML, while the PK isoenzyme pattern was normal. Administration of antitumor antibiotics to AL decreased G6PD and 6PGD activities (P < 0.05). ADA2 was expressed in MNC of AML (M4 and M5) and in RBC of CMML, but not expressed in ALL (P < 0.001).


CONCLUSIONS
Determination of enzymes may be used as prognostic parameters in CML,a predictor of antitumor drug resistance in acute leukemia, and a differential parameter between MDS-RA and aplastic anemia and between CML and CMML.
Lin
G
G
Huashan Hospital, Shanghai Medical University, Shanghai 200040.
Xie
Y
Y
Liang
X
X
Wu
X
X
Liu
W
W
Yi
M
M
Lin
P
P
Wang
Q
Q
D000293:Adolescent; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D007938:Leukemia / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D055815:Young Adult
D000970:Antineoplastic Agents; D007527:Isoenzymes