title: Influence of mitochondrial membrane fatty acid composition on proton leak and H2O2 production in liver.
abstract: Mitochondrial membrane fatty acid composition has been proposed to play a role in determining mitochondrial proton leak rate. The purpose of this study was to determine if feeding rats diets with different fatty acid sources produces changes in liver proton leak and H(2)O(2) production. Six-month-old male FBNF(1) rats were fed diets with a primary fat source of either corn or fish oil for a 6-month period. As expected, diet manipulations produced substantial differences in mitochondrial fatty acid composition. These changes were most striking for 20:4n6 and 22:6n3. However, proton leak and phosphorylation kinetics as well as lipid and protein oxidative damage were not different (P > 0.10) between fish and corn oil groups. Metabolic control analysis, however, did show that control of both substrate oxidation and phosphorylation was shifted away from substrate oxidation reactions to increased control by phosphorylation reactions in fish versus corn oil groups. Increased mitochondrial H(2)O(2) production was observed in corn versus fish oil-fed rats when mitochondria were respiring on succinate alone or on either succinate or pyruvate/malate in the presence of antimycin A. These results show that mitochondrial H(2)O(2) production and the regulation of oxidative phosphorylation are altered in liver mitochondria from rats consuming diets with either fish or corn oil as the primary lipid source.
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. jjramsey@ucdavis.edu
authors.forename: Jon J
authors.initials: JJ
authors.lastname: Ramsey
authors.forename: Mary-Ellen
authors.initials: ME
authors.lastname: Harper
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Humble
authors.forename: Edward K
authors.initials: EK
authors.lastname: Koomson
authors.forename: Jesmon J
authors.initials: JJ
authors.lastname: Ram
authors.forename: Lisa
authors.initials: L
authors.lastname: Bevilacqua
authors.forename: Kevork
authors.initials: K
authors.lastname: Hagopian
mesh_terms: D000818:Animals; D001835:Body Weight; D002462:Cell Membrane / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000378:metabolism*; D009929:Organ Size; D010085:Oxidative Phosphorylation; D018384:Oxidative Stress; D010766:Phosphorylation; D051381:Rats
chemical_list: D005227:Fatty Acids; D006861:Hydrogen Peroxide