title: Plant regeneration from epicotyl explant of Jatropha curcas.
abstract: In vitro epicotyl explants from Jatropha curcas were cultured on MS medium with indole-3-butyric acid (IBA) and 6-benzyladenine (BA). Adventitious buds were directly induced from the surface of epicotyl explants under the condition with the combinations of IBA 0.1 mg/L and BA 0.2-0.7 mg/L. Of which IBA 0.1 mg/L and BA 0.5 mg/L induced the highest regeneration frequency. Shoot regeneration from callus required the combinations of IBA 0.5 mg/L with BA 0.1 mg/L, IBA 0.5 mg/L with BA 0.2 mg/L and IBA 1.0 mg/L with BA 0.5 mg/L, and the most suitable combination was IBA 1.0 mg/L and BA 0.5 mg/L. The health adventitious buds and regenerated shoots could be rooted on growth regulator-free MS medium. Regenerated plants with well developed shoots and roots were successfully transferred to greenhouse, without visible detectable variation.
authors.affiliation: Sichuan Key Lab of Resource Biology and Pharmaceutical Engineering, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.forename: Wei-Da
authors.initials: WD
authors.lastname: Lu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liao
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Pan
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D007211:Indoles / Q000494:pharmacology; D031286:Jatropha / Q000502:physiology*; D007701:Kinetin; D011687:Purines; D012038:Regeneration
chemical_list: D001593:Benzyl Compounds; D007211:Indoles; D011687:Purines; C014612:indolebutyric acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin