[Apoptosis of acute myeloid leukemic cells induced by human recombinant soluble tumor necrosis factor-related apoptosis inducing ligand (TRAIL)].
In order to investigate the effect of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) on acute myeloid leukemic (AML) cells, BMMNC or PBMNC of 39 cases of AML (patient group) and 21 normal persons (control group) were isolated; the cytotoxic effects of TRAIL at various concentration on the cells were evaluated by MMT, apoptosis were detected by flow cytometry. The results showed that TRAIL of various concentration could kill AML cells in various degrees, but had no killing effect on normal cells. It is concluded that TRAIL can kill AML cells through inducing apoptosis, and TRAIL has a promising prospect in clinical use.
Wang
Hong-Xiang
HX
Department of Hematology, The Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ljwhx@21cn.com
Zou
Ping
P
Zhao
Shi
S
D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000494:pharmacology; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand