title: Mobilization of the active MITE transposons mPing and Pong in rice by introgression from wild rice (Zizania latifolia Griseb.).
abstract: Hybridization between different species plays an important role in plant genome evolution, as well as is a widely used approach for crop improvement. McClintock has predicted that plant wide hybridization constitutes a "genomic shock" whereby cryptic transposable elements may be activated. However, direct experimental evidence showing a causal relationship between plant wide hybridization and transposon mobilization has not yet been reported. The miniature-Ping (mPing) is a recently isolated active miniature inverted-repeat transposable element transposon from rice, which is mobilized by tissue culture and gamma-ray irradiation. We show herein that mPing, together with its putative transposase-encoding partner, Pong, is mobilized in three homologous recombinant inbred lines (RILs), derived from hybridization between rice (cultivar Matsumae) and wild rice (Zizania latifolia Griseb.), harboring introgressed genomic DNA from wild rice. In contrast, both elements remain immobile in two lines sharing the same parentage to the RILs but possessing no introgressed DNA. Thus, we have presented direct evidence that is consistent with McClintock's insight by demonstrating a causal link between wide hybridization and transposon mobilization in rice. In addition, we report an atypical behavior of mPing/Pong mobilization in these lines, i.e., the exclusive absence of footprints after excision.
authors.affiliation: Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Shan
authors.forename: Zhenlan
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Dong
authors.forename: Yongming
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Lin
authors.forename: Likun
authors.initials: L
authors.lastname: Long
authors.forename: Fangpu
authors.initials: F
authors.lastname: Han
authors.forename: Yingshan
authors.initials: Y
authors.lastname: Dong
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D017343:Genes, Plant; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D010940:Plant Proteins