[Generation and identification of rice T-DNA insertional mutant lines].
By using the matured embryos of Japonica rice variety Zhonghua No. 11 as explants, rice transformation was performed by Agrobacterium-mediated co-cultivation method, resulting in 1489 independent transgenic rice plants that carry a T-DNA insertion. Genomic DNA gel-blot and PCR analyses showed that 69.8% of the total lines contain the inserted T-DNA. The flanking sequence of T-DNA in transgenic rice plants was analyzed using Tail-PCR. In addition, we have evaluated 1066 T1 transgenic lines on heading days, plant height and panicles per hill, and found different types of mutants from a number of lines.
Yan
Shuang-Yong
SY
Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China.
Zhi
Qing-Wen
QW
Liu
Xin-Jie
XJ
Zhang
Hong-Wei
HW
Tan
Zhen-Bo
ZB
Li
Shi-Gui
SG
D004269:DNA, Bacterial / Q000235:genetics*; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; C036483:T-DNA