Cytotoxic effects induced by oxidative stress in cultured mammalian cells and protection provided by Hsp27 expression.
There is currently great interest in the development of methods to analyze intracellular redox state and the cellular damages generated by oxidative stress. General methods for analyzing reactive oxygen species and glutathione level are presented together with more recently developed protocols to analyze the consequences of oxidative stress on the oxidation of macromolecules. Finally, techniques to study modalities of constitutive expression of Hsp27 in mammalian cells are considered as well as methods used to determine the protective activity of this small heat shock protein against the deleterious effects induced by oxidative stress.
Arrigo
André-Patrick
AP
Laboratoire Stress Oxydant, Chaperons et Apoptose, Centre de Génétique Moléculaire et Cellulaire, CNRS UMR 5534, Université Claude Bernard Lyon-1, Villeurbanne 69622, France. arrigo@univ-lyon1.fr
Firdaus
Wance J J
WJ
Mellier
Grégory
G
Moulin
Maryline
M
Paul
Catherine
C
Diaz-latoud
Chantal
C
Kretz-remy
Carole
C
D000818:Animals; D017304:Annexin A5 / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D016923:Cell Death; D002478:Cells, Cultured; D019281:Dimerization; D004789:Enzyme Activation; D055551:HSP27 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
D017304:Annexin A5; D000975:Antioxidants; D055551:HSP27 Heat-Shock Proteins; D017382:Reactive Oxygen Species