Profiling gene expression in kidney development.
Rapid determination of the expression levels of all genes can be achieved with microarrays. This technology is beginning to revolutionize the study of kidney development. Genome-wide expression studies of the developing rat, mouse and human kidneys have found thousands of expressed genes, with many hundreds showing changes in expression as a function of developmental time. The resulting gene expression profiles provide an important discovery function, identifying new genes and pathways not previously implicated in kidney organogenesis. In combination with microdissection techniques, microarrays further extend global gene expression analysis to discrete components or cell types within the kidney. The resulting detailed molecular portrait of normal kidney development provides a baseline for studies of the many mouse mutants available with abnormal kidney development.
Patterson
Larry T
LT
Division of Nephrology, Children's Hospital Research Foundation, Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.
Potter
S Steven
SS
D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007668:Kidney / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D014513:Ureter / Q000196:embryology / Q000254:growth & development / Q000378:metabolism