Studies on the membrane interactions of the cyclotides kalata B1 and kalata B6 on model membrane systems by surface plasmon resonance.
In this study we have demonstrated the interactions of kalata B1 and its naturally occurring analogue kalata B6 with five model lipid membranes and have analyzed the binding kinetics using surface plasmon resonance. Two kalata peptides showed a higher affinity for the phosphatidylethanolamine-containing membranes, indicating that the peptides would bind selectively to bacterial membranes. Also we have optimized the procedure for the immobilization of five liposome mixtures and have shown that the procedure provides reproducible levels of immobilized liposomes and could be used to screen the selective binding of putative antimicrobial peptides to model mammalian or microbial phospholipid membranes.
Kamimori
Hiroshi
H
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia. hiroshi.kamimori@shinonogi.co.jp
Hall
Kristopher
K
Craik
David J
DJ
Aguilar
Marie-Isabel
MI
D047169:Cyclotides / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D008567:Membranes, Artificial; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D020349:Surface Plasmon Resonance
D047169:Cyclotides; D008081:Liposomes; D008567:Membranes, Artificial; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C091930:kalata B1; C501913:kalata B6; C483858:phosphatidylethanolamine