Liquid-liquid extraction of alpha-lactalbumin using reverse micellar organic solvent.
The liquid-liquid extraction of alpha-lactalbumin based on reverse micellar organic solvents was investigated. Forward extraction of the protein in the reverse micellar organic phase from aqueous feed solutions was strongly dependent on the initial pH of the feed solution and the complete forward extraction of 0.03 mM alpha-lactalbumin was successfully achieved at pH 6.0. The forward extraction percentage steeply decreased with increasing KCl concentration, while in the NaCl system the forward extraction was independent on the salt concentration below 1 M. From the circular dichroic measurement, higher order structure of the recovered alpha-lactalbumin through the extraction process was well preserved.
Naoe
Kazumitsu
K
Department of Chemical Engineering, Nara National College of Technology, 22 Yata, Yamato-Koriyama, Nara 639-1080, Japan. naoe@chem.nara-k.ac.jp
Noda
Kazuki
K
Konishi
Taiji
T
Kawagoe
Mikio
M
Imai
Masanao
M
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007768:Lactalbumin / Q000302:isolation & purification*; D008823:Micelles; D008892:Milk / Q000737:chemistry; D009994:Osmolar Concentration; D012997:Solvents
D007202:Indicators and Reagents; D008823:Micelles; D012997:Solvents; D007768:Lactalbumin