[In vitro infection of the human T-cell line with the hepatitis C virus].
A human T-cell line (Molt-4) was infected with an hepatitis C virus RNA (HCV- RNA) positive serum for 12 hours and incubated continously for 1-28 days. A productive infection was demonstrated by polymerase chain reaction amplification. Viral plus-stranded RNA was detectable in Molt-4 on the 1st, 2nd, 3rd day and the 8th day post infection. Viral minus-stranded RNA was detectable on the 2nd day and 8th day post infection.
Ye
J
J
Dept. of Infection, Union Hospital of Tongji Medical University, Wuhan 430022.
Goeser
T
T
D002460:Cell Line; D016174:Hepacivirus / Q000235:genetics* / Q000254:growth & development*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics*; D013601:T-Lymphocytes / Q000821:virology*
D012367:RNA, Viral