[Differentially expressed genes in human giant-cell lung cancer lines with different metastatic potentials].
OBJECTIVE
To screen genes differentially expressed in two human giant-cell lung cancer lines of same origin but with different metastasis potentials.


METHODS
Suppression subtractive hybridization (SSH) was done twice on two giant-cell lung cancer lines, PLA-801C and PLA-801D (hereafter abbreviated as C and D), of same origin but with low (C) and high (D) metastatic potentials. In the first round, SSH C was used as tester and D as driver, while in the second round, the tester and driver were interchanged. The sequences acquired from both rounds of SSH were spotted on glass slides respectively and screened by hybridizing with two-color fluorescence probes. Clones that had different expression levels on chips were also confirmed by RNA dot blot or Northern blot.


RESULTS
There were 16 sequences with high expression in C as compared to those in D, and 79 sequences with high expression in D compared to those in C. After sequencing, most of them were found to be highly homologous to those encoding the following proteins: (1) cytokines and their receptors; (2) kinases and related proteins; (3) other proteins including enzymes, heat shock proteins, receptors, proteins of cell skeleton and mitochondria, products of oncogenes, etc; (4) some proteins deduced from gene sequences with yet unknown functions.


CONCLUSIONS
The alterations in expression of some known genes, including HSP70, AXL receptor tyrosine kinase and 14-3-3zeta, might have impact on metastasis of giant-cell lung cancer. Whether some differentially expressed genes newly revealed are metastasis-related needs further study.
Zhang
Jin-qiang
JQ
Department of Pathobiology, Institute of Basic Medical Science, Academy of Military Medical Sciences, Beijing 100850, China.
Wang
Yan
Y
Wang
Tao
T
Du
Zhi-yan
ZY
Xu
Yuan-ji
YJ
Lu
Ying-lin
YL
D048948:14-3-3 Proteins / Q000378:metabolism; D018286:Carcinoma, Giant Cell / Q000235:genetics* / Q000378:metabolism / Q000556:secondary*; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D009362:Neoplasm Metastasis; D009693:Nucleic Acid Hybridization; D015513:Oncogene Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D000094963:Axl Receptor Tyrosine Kinase
D048948:14-3-3 Proteins; D018840:HSP70 Heat-Shock Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases; D000094963:Axl Receptor Tyrosine Kinase; C000722289:AXL protein, human