Fast dipstick dye immunoassay for detection of immunoglobulin G (IgG) and IgM antibodies of human toxoplasmosis.
A dipstick dye immunoassay (DDIA) was developed to detect immunoglobulin G (IgG) or IgM antibodies of toxoplasmosis infection in humans. The assays employ a blue colloidal dye particles (D-1) conjugated to sheep anti-human IgG and rabbit anti-human IgM as the visualizing agents and a soluble antigen of tachyzoites of Toxoplasma gondii strain RH (TSA) as the detective antigen. The mixture of dye-labeled anti-human antibody-special human antibody was captured by TSA onto a nitrocellulose membrane dipstick by means of immunochromatography. The assays are rapid (the whole test can be completed within 15 min), simple, and cheap, and they don't require any equipment. They are sensitive and specific for the detection of anti-Toxoplasma IgG or IgM antibodies and generally agree closely with the results from the enzyme-linked immunosorbent assay. The assays are especially suitable for field applications.
Jin
Si
S
Institute of Medical Molecular Biology, Nanjing Medical University, Nanjing, People's Republic of China.
Chang
Zhu Yin
ZY
Ming
Xu
X
Min
Cao Li
CL
Wei
He
H
Sheng
Liang You
LY
Hong
Guan Xiao
GX
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004396:Coloring Agents; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D004396:Coloring Agents; D007074:Immunoglobulin G; D007075:Immunoglobulin M