High-throughput mass spectrometer using atmospheric pressure ionization and a cylindrical ion trap array.
The analytical performance of an atmospheric pressure sampling, multiple-channel, high-throughput mass spectrometer was investigated using samples of a variety of types. The instrument, based on an array of cylindrical ion traps, was built with four independent channels and here is operated using two fully multiplexed channels (sources, ion optics, ion traps, detectors) capable of analyzing different samples simultaneously. Both channels of the instrument were incorporated within the same vacuum system and operated using a common set of control electronics. A multichannel electrospray ionization source was assembled and used to introduce samples including solutions of organic compounds, peptides, and proteins simultaneously into the instrument in a high-throughput fashion. Cross-talk between the channels of the instrument occurred in the detection system and could be minimized to 1-2% using shielding between detector channels. In this initial implementation of the instrumentation, an upper mass/charge limit of approximately 1300 Th was observed (+13 charge state of myoglobin) and unit mass/charge resolution was achieved to approximately 800 Th. The rather limited dynamic range (2-3 orders of magnitude for low-concentration analytes) is due to cross-talk contributions from more concentrated species introduced into a different channel. Analysis of mixtures of alkylamines and peptides is demonstrated, but analysis of mixtures with a wide spread in mass/charge ratios was not possible due to mass discrimination in the ion optics. Further refinement of the vacuum system and ion optics will allow the addition of more channels of parallel mass analysis and facilitate applications in fields such as proteomics and metabolomics.
Misharin
Alexander S
AS
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.
Laughlin
Brian C
BC
Vilkov
Andrey
A
Takáts
Zoltán
Z
Ouyang
Zheng
Z
Cooks
R Graham
RG
D000588:Amines / Q000302:isolation & purification; D000596:Amino Acids / Q000032:analysis; D001274:Atmospheric Pressure; D002082:Butylamines / Q000302:isolation & purification; D004809:Ephedrine / Q000302:isolation & purification; D024841:Fluoroquinolones / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D009211:Myoglobin / Q000302:isolation & purification; D009842:Oligopeptides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods
D000588:Amines; D000596:Amino Acids; D002082:Butylamines; D024841:Fluoroquinolones; D009211:Myoglobin; D009842:Oligopeptides; D011506:Proteins; C036355:tributylamine; C009317:stearylamine; D004809:Ephedrine; C053091:lomefloxacin; C003935:dodecylamine