[Leptin as a survival enhanced-factor in grainy fat transplantation].
OBJECTIVE
This experiment is to test the survival enhance effect of leptin in grainy fat transplantation.


METHODS
The 0.6 g grainy fat was obtained from the Wistar mouse subcutaneous tissue in the abdomen. It was then mixed with 0.9% NS and 20 ng/ml leptin and transplanted in the head subcutaneously. The transplanted tissue was taken out after 10, 20 and 40 days. After being weighed, the tissue was dyed with HE staining and VEGF staining. The data was analyzed with SPSS software.


RESULTS
The fibrous membrane around the tissue in leptin group was thin and there was less fat cell necrosis, compared with the control group. The remained-weight rate was 108.3%, 83.3% and 66.3% in 10, 20 and 40 days after the surgery. The differences were statistic significant between the leptin groups and the control (P < 0.05). The examinations of the HE and VEGF stain showed that there was the rich vascularization in leptin treated tissue.


CONCLUSIONS
Leptin could enhance the survival of the transplanted grainy fat due to its angiogenesis effect.
Wang
You-bin
YB
Plastic Surgery Department of Peking Union Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Qi
Ke-ming
KM
Zhao
Min
M
Zhuang
Qiang
Q
D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D005260:Female; D006801:Humans; D020738:Leptin / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D016019:Survival Analysis; D013997:Time Factors; D014184:Transplantation, Homologous; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D020738:Leptin; D042461:Vascular Endothelial Growth Factor A