[Clinical study of hemostatic molecular markers expression in patients with cancer].
OBJECTIVE
To study the changes of hemostatic molecular markers in patients with gastric or intestinal cancer for elucidating their clinical significance.


METHODS
The plasma levels of tissue factor (TF), thrombin antithrombin complex (TAT), tissue plasminogen activator (t-PA), urokinase plasminogen activator (u-PA), urokinase plasminogen activator receptor (u-PAR) and plasmin antiplasmin complex (PAP) were measured by ELISA. Gene transcription of TF, t-PA, u-PA mRNA were detected by real-time RT-PCR.


RESULTS
The plasma levels of TF, TAT, u-PA, u-PAR and PAP were elevated in gastric or intestinal cancer patients (P < 0.05), while u-PA, u-PAR remarkably increased in patients with local infiltration, lymph node involvement or distal metastasis (P < 0.01). Plasma level of TF, TAT, PAP were remained higher than control even after surgery. TF, u-PA mRNA were higher (P < 0.01) and t-PA was lower (P > 0.05) in gastric or intestinal cancer compared to normal tissue.


CONCLUSIONS
Their existed over expression of TF and u-PA, increasing formation of thrombin and plasminogen in gastric or intestinal cancer patients. Hypercoagulability and hyperfibrinolysis were important factors related with metastasis potential of gastric or intestinal cancer. t-PA may be a character of well differentiated tissue. Quantitative detection of TF and u-PA gene expression by the method of real-time RT-PCR is feasible for them as observation markers in gastric or intestinal cancer patients.
Wu
Fang
F
Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Qu
Bin
B
Wang
Xue-Feng
XF
Yang
Chen-Min
CM
Wang
Hong-Li
HL
D000328:Adult; D001777:Blood Coagulation; D005260:Female; D006801:Humans; D007414:Intestinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000235:genetics / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D013925:Thromboplastin; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator