title: Tea catechin, epigallocatechin-3-gallate suppresses myosin II regulatory light chain phosphorylation.
abstract: Phosphorylation of myosin II regulatory light chain (MRLC) is critical event for many cellular processes including muscle contraction, mytosis, migration, and exocytosis. Epigallocatechin-3-O-gallate (EGCG) is a major polyphenolic compound of green tea and has various physiological functions. We found that EGCG disrupted stress fibers and suppressed the MRLC phosphorylation in HeLa cells. To elucidate the mechanism for the suppressive effect on the phosphorylation, we examined the effect of various inhibitors for kinases that modulate MRLC phosphorylation. None of the inhibitors mimic the activity of EGCG. These results suggest that EGCG is a compound that can suppress MRLC phosphorylation.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Umeda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tachibana
authors.forename: Koji
authors.initials: K
authors.lastname: Yamada
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D018994:Myosin Light Chains / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D013314:Stress, Mechanical; D013662:Tea
chemical_list: D000975:Antioxidants; D018994:Myosin Light Chains; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate