Lanostanoid triterpenes from Laetiporus sulphureus and apoptosis induction on HL-60 human myeloid leukemia cells.
A new lanostanoid triterpene, 3-oxosulfurenic acid (1), together with three known triterpenes (3, 4, and 7) were isolated from the fruit bodies of Laetiporus sulphureus. Cytotoxicity of these compounds and their derivatives (2, 5, and 6) was evaluated on HL-60 cells. Further studies revealed that acetyl eburicoic acid (5) was the most potent apoptosis inducer. Apoptosis was accompanied by both the activation of caspase-3 and the fragmentation of poly(ADP-ribose) polymerase-1 and was also associated with an early release of cytochrome c from the mitochondria.
León
Francisco
F
Instituto de Productos Naturales y Agrobiología, CSIC, Instituto Universitario de Bio-Orgánica Antonio González, Avenida Astrofísico F. Sánchez 3, 38206 La Laguna, Tenerife, Spain.
Quintana
José
J
Rivera
Augusto
A
Estévez
Francisco
F
Bermejo
Jaime
J
D017209:Apoptosis / Q000187:drug effects*; D003105:Colombia; D045304:Cytochromes c / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007951:Leukemia, Myeloid; D008928:Mitochondria / Q000201:enzymology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011124:Polyporaceae / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
C496939:3-oxosulfurenic acid; D014315:Triterpenes; D007810:Lanosterol; D045304:Cytochromes c; D011065:Poly(ADP-ribose) Polymerases