Noncovalent interaction of MNSFbeta, a ubiquitin-like protein, with histone 2A.
Monoclonal nonspecific suppressor factor (MNSF), a lymphokine produced by murine T cell hybridoma, possesses pleiotrophic antigen-nonspecific suppressive functions. A cDNA clone encoding MNSFbeta, an isoform of the MNSF, has been isolated and characterized. MNSFbeta cDNA encodes a fusion protein consisting of a ubiquitin-like segment (Ubi-L) and ribosomal protein S30. Most recently, we observed that Ubi-L covalently conjugates to Bcl-G, a novel pro-apoptotic protein. In this study, we observed that Ubi-L noncovalently and specifically binds to histone 2A. The maximum binding was observed at a molar ratio equal to 1 for GST-Ubi-L and 2 for histone 2A. Ubi-L formed complex with histone 2A in the presence of 1% Triton X-100. Free Ubi-L was detected in nuclei from unstimulated murine helper T cell line, D10. The increased amounts of free Ubi-L and some Ubi-L adducts were observed in nuclei from mitogen-activated D10 cells. Interestingly, two Ubi-L adducts were unique to the chromatin fraction of nuclei from the activated D10 cells.
Nakamura
Morihiko
M
Cooperative Medical Research Center, Shimane University, 89-1 Enya-cho, Izumo 693-8501, Japan. nkmr0515@med.shimane-u.ac.jp
Tanigawa
Y
Y
D000818:Animals; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013491:Suppressor Factors, Immunologic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014452:Ubiquitins / Q000737:chemistry*
D006657:Histones; D011993:Recombinant Fusion Proteins; D013491:Suppressor Factors, Immunologic; D014452:Ubiquitins; C072169:monoclonal nonspecific suppressor factor