Unilateral kidney-selective gene transfer following the administration of naked plasmid DNA to the kidney surface in mice.
We developed a gene transfer following the administration of naked plasmid DNA (pDNA) to the kidney surface in mice, and found that the luciferase levels produced in the applied kidney were significantly higher than those produced in another kidney. In contrast, stable renal gene expression was not observed in the case of intraperitoneal or intravenous administration of pDNA. The level of gene expression after instillation of pDNA to the kidney surface reached maximum at 12 h and gradually diminished thereafter. The production of luciferase was saturated at 5 microg of pDNA, and was not affected by instillation volume. Furthermore, pDNA uptake from the kidney surface was proved by in situ experiments using a glass-made diffusion cell. We demonstrated a novel unilateral kidney-selective gene transfer following the administration of naked pDNA to the kidney surface in mice.
Hirayama
Ryu
R
Graduate School of Biomedical Sciences, Nagasaki University, Japan.
Nishida
Koyo
K
Fumoto
Shintaro
S
Nakashima
Mikiro
M
Sasaki
Hitoshi
H
Nakamura
Junzo
J
D000818:Animals; D004305:Dose-Response Relationship, Drug; D018014:Gene Transfer Techniques; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage*
D019444:Vaccines, DNA