title: Cloning and analysis of human Apg16L.
abstract: Autophagy is an intracellular bulk degradation system, which delivers cytoplasmic components to the lysosome/vacuole. In yeast and mammalian cells, the Apg12-Apg5 conjugate, together with Apg16, form a multimeric complex, which plays an essential role in autopihageosome formation. By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA encoding a putative protein with 607 amino acid residues, which shows 90% identity and 93% similarity to mouse Apg16L. This protein, designated human Apg16L, contains a coiled-coil domain and a motif with seven WD repeats, which are also shared by mouse Apg16L. Database searching revealed that Apg16L is mapped to chromosome 2q37.1 and there exist at least four splice variants.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Huarui
authors.initials: H
authors.lastname: Zheng
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Jixi
authors.initials: J
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Mengru
authors.initials: M
authors.lastname: Ren
authors.forename: Qi
authors.initials: Q
authors.lastname: Lu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D001343:Autophagy / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D020033:Protein Isoforms