title: Polyphosphate kinase 1 and the ocular virulence of Pseudomonas aeruginosa.
abstract: OBJECTIVE
  To determine the role of polyphosphate kinase 1 (PPK1) in the ocular virulence of Pseudomonas aeruginosa.
  
  
  METHODS
  Using a mouse model of infection, P. aeruginosa strains PAO1, PAOM5 (an isogenic mutant of PAO1 deficient in PPK1), and PAOM5+PPK1 (the mutant complemented with PPK1 on plasmid pHEPAK11) were compared for ocular virulence. These strains were also characterized with respect to traits associated with survival and pathogenicity in an ocular environment.
  
  
  RESULTS
  The PPK1-deficient strain PAOM5 was significantly less virulent than either wild-type PAO1 or the complemented mutant (P <0.016). Loss of virulence was not associated with serum sensitivity or diminished adherence to the cornea. However, PAOM5 has an increased susceptibility to oxidative stress and was cleared from corneal tissue significantly better (P <0.006) than either the wild-type or restored strain. Furthermore, the PPK1-deficient mutant produced significantly less (P <0.022) pyocyanin.
  
  
  CONCLUSIONS
  PPK1 is essential for a successful ocular infection by P. aeruginosa. The loss of ocular virulence is probably due to the dysregulation of multiple genes, including those responsible for stress response.
authors.affiliation: Department of Microbiology, Immunology, and Parasitology, LSU Health Sciences Center, New Orleans, Louisiana 70112, USA.
authors.forename: Quinn M
authors.initials: QM
authors.lastname: Parks
authors.forename: Jeffery A
authors.initials: JA
authors.lastname: Hobden
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D015169:Colony Count, Microbial; D003316:Corneal Diseases / Q000382:microbiology*; D019573:Epithelium, Corneal / Q000378:metabolism / Q000382:microbiology; D015818:Eye Infections, Bacterial / Q000382:microbiology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000172:deficiency / Q000502:physiology*; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000472:pathogenicity*; D011710:Pyocyanine / Q000378:metabolism; D044967:Serum / Q000502:physiology; D014774:Virulence
chemical_list: D011710:Pyocyanine; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase