title: First proteomic analysis of Legionella pneumophila based on its developing genome sequence.
abstract: The first proteomic analysis of the respiratory pathogen Legionella pneumophila ATCC 33152 is presented in this report. Two-dimensional gel electrophoresis of total cell extracts was carried out. In total, 130 protein spots were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MS) or by quadruple time-of-flight tandem MS, including proteins correlated with virulence. For the first time, proteins of L. pneumophila were identified using mass spectrometric methods and mapped on a two-dimensional gel; this will be of considerable use for comparison of protein expression profiles of L. pneumophila wild-type and knock-out mutant strains and of L. pneumophila grown under different conditions.
authors.affiliation: Laboratory of Bacteriology, Rega Instituut, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium.
authors.forename: Ilya
authors.initials: I
authors.lastname: Lebeau
authors.forename: Elke
authors.initials: E
authors.lastname: Lammertyn
authors.forename: Emmy
authors.initials: E
authors.lastname: De Buck
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Maes
authors.forename: Nick
authors.initials: N
authors.lastname: Geukens
authors.forename: Lieve
authors.initials: L
authors.lastname: Van Mellaert
authors.forename: Lut
authors.initials: L
authors.lastname: Arckens
authors.forename: Jozef
authors.initials: J
authors.lastname: Anné
authors.forename: Stefan
authors.initials: S
authors.lastname: Clerens
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D016952:Legionella pneumophila / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D040901:Proteomics; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D020543:Proteome