Genetic characterization of high-level gentamicin-resistant strains of Enterococcus faecalis in Iran.
The prevalence of resistance to high levels of gentamicin among 182 isolates of Enterococcus faecalis from 2 Iranian hospitals was 42%. Gentamicin resistance was associated with conjugative plasmids (>70 kb) in most strains. Fingerprinting using EcoRI and HindIII showed genetic variation among these plasmids and gave evidence of nosocomial outbreaks and persistence of infection in different wards of the study hospitals, as well as transfer of plasmids between genetically diverse isolates. Using EcoRI, hospital-based specific plasmid fingerprints were detected for the isolates that had previously proved to be unrelated by multilocus enzyme electrophoresis, suggesting the persistence of related plasmids at each hospital, though minor changes in these related plasmids could be detected with HindIII.
Feizabadi
Mohammad Mehdi
MM
Department of Biological Science, Alzahra University, Vanak, Tehran, Iran. medifeiz@yahoo.com
Asadi
Sorour
S
Zohari
Maryam
M
Gharavi
Sara
S
Etemadi
Gelavizh
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003227:Conjugation, Genetic; D016172:DNA Fingerprinting; D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005839:Gentamicins / Q000494:pharmacology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007492:Iran; D008297:Male; D017720:Molecular Epidemiology; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005839:Gentamicins; D015246:Deoxyribonuclease EcoRI