Immobilization of amine-modified oligonucleotides on aldehyde-terminated alkanethiol monolayers on gold.
Chemistry is described for the fabrication of DNA arrays on gold surfaces. Alkanethiols modified with terminal aldehyde groups are used to prepare a self-assembled monolayer (SAM). The aldehyde groups of the monolayer may be reacted with amine-modified oligonucleotides or other amine-bearing biomolecules to form a Schiff base, which may then be reduced to a stable secondary amine by treatment with sodium cyanoborohydride. The surface modifications and reactions are characterized by polarization modulation Fourier transform infrared reflection absorption spectroscopy (PM-FTIRRAS), and the accessibility, binding specificity, and stability of the DNA-modified surfaces are demonstrated in hybridization experiments.
Peelen
Dora
D
Department of Chemistry, 1101 University Avenue, University of Wisconsin, Madison, WI 53706-1396, USA.
Smith
Lloyd M
LM
D000447:Aldehydes / Q000737:chemistry*; D000588:Amines / Q000737:chemistry*; D001483:Base Sequence; D006046:Gold / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D000447:Aldehydes; D000588:Amines; D009841:Oligonucleotides; D006046:Gold