[Cloning and sequence analysis of cDNA encoding PPAR from goose].
The peroxisome proliferator activated receptor (PPAR) belongs to a large family of nuclear receptors. This study was designed to clone and sequence analysis of cDNA encoding PPAR from goose. The RT-PCR method was developed to clone the cDNA, and the lengths of cDNA encoding PPARalpha and PPARgamma were 1407bp and 1428bp respectively. The cDNAs of the two genes were cloned and sequenced for the first time. The identities of CDS of PPARalpha and PPARgamma gene were 87.43% and 92.00% by homologous comparison among goose and other five species, and that were 93.38% and 96.95% in amino acid sequences. The further analysis among seventeen species including goose showed that the identities of PPAR genes were low(66.18%) among different sub-type (alpha,gamma,beta) of PPAR genes and that was high for the same sub-type of PPAR genes: PPARalpha, PPARgamma and PPARbeta (or PPARdelta) were 84.80%, 86.23% and 87.36% respectively. The results showed that these two genes are conservative in the process of evolution and has important physiological function for the growth and development of birds and mammals. The results of the present study will benefit the further study of relationship between PPAR genes and the growth and development, especially in fat metabolism of goose.
Meng
He
H
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. menghe@sjtu.edu.cn
Li
Hui
H
Wang
Xiang-Yu
XY
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005777:Geese / Q000235:genetics*; D008969:Molecular Sequence Data; D047493:PPAR alpha / Q000235:genetics; D047495:PPAR gamma / Q000235:genetics; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D018076:DNA, Complementary; D047493:PPAR alpha; D047495:PPAR gamma; D047492:Peroxisome Proliferator-Activated Receptors