title: [Establishment of a new HBV genotyping method with PCR-RBD and its application].
abstract: OBJECTIVE
  Using PCR-RDB to establish a new method for HBV genotyping, and to survey the distribution of HBV genotypes in the Foshan area.
  
  
  METHODS
  Biotin-labeled primers for amplification of HBV region X (nt1550-1789) were used to amplify extracted HBV DNA. HBV was genotyped by hybridization of the PCR products with immobilized specific probes (genotype A to F) on C membrane. Color development was achieved by adding POD and TMB. A judgment was made according to color reactions. The reliability of this new method was verified by gene sequencing. 300 samples of HBV DNA-positive sera from the Foshan area were genotyped using this assay.
  
  
  RESULTS
  Of the 300 sera genotyped by PCR-RBD, 147 (49.0%) cases were genotype B, 136 (45.3%) were genotype C, 1 (0.3%) genotype D, and 12 (4.0%) were mixtures of genotype B and C, and 4 (1.3%) were mixtures of genotype C and D. No genotype A, E or F were found. The results of PCR-RDB genotyping were consistent with the results obtained with sequence analysis.
  
  
  CONCLUSIONS
  This newly established HBV genotyping system proved to be sensitive, specific, precise and economic, and should be suitable for clinical practice and epidemic study. The results of HBV genotyping show that genotype B and C are the predominant genotypes in the Foshan area.
authors.affiliation: Institute of Clinical/Medical Research, the First People's Hospital of Foshan, Foshan, Guangdong 528000, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Jin-huan
authors.initials: JH
authors.lastname: Cui
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Si
authors.forename: Jia-ju
authors.initials: JJ
authors.lastname: Tan
authors.forename: Pei-yuan
authors.initials: PY
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D004279:DNA, Viral