High-throughput profiling of dietary polyphenols and their metabolites by HPLC-ESI-MS-MS in human urine.
Polyphenols in biological fluids are generally estimated by HPLC with UV, electrochemical or fluorimetric detection. We describe herein a method specially developed to estimate biomarkers of polyphenol consumption in human urine. We simultaneously quantified 15 polyphenols and their related compounds in human urine using an HPLC coupled with electrospray ionization mass-mass (HPLC-ESI-MS-MS) method with an analytical run time of 6 min. The method has been validated with respect to linearity, precision, and accuracy in intra- and inter-day assays. It has been applied to human urine samples collected from volunteers after consumption of different polyphenol-rich beverages in controlled conditions.
Ito
Hideyuki
H
Unité des Maladies Métaboliques et Micronutrients, INRA, 63122 Saint-Genès-Champanelle, France. hito@cc.okayama-u.ac.jp
Gonthiera
Marie-Paule
MP
Manach
Claudine
C
Morand
Christine
C
Mennen
Louise
L
Rémésy
Christian
C
Scalbert
Augustin
A
D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005419:Flavonoids / Q000493:pharmacokinetics* / Q000652:urine*; D006801:Humans; D010636:Phenols / Q000493:pharmacokinetics* / Q000652:urine*; D059808:Polyphenols; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols