title: CD4+CD25+ regulatory T cells control induction of autoimmune hemolytic anemia.
abstract: Autoimmune hemolytic anemia (AIHA) is the result of increased destruction of red blood cells (RBCs) due to the production of autoantibodies, and it can be life-threatening. To study the mechanisms that trigger AIHA, we used the Marshall-Clarke and Playfair model of murine AIHA, in which mice repeatedly immunized with rat RBCs develop erythrocyte autoantibodies as well as rat-specific alloantibodies. We analyzed the role of CD25(+) T-regulatory subsets in controlling AIHA in C57/Bl6 mice using antibody depletion studies. Treatment with anti-CD25 antibody but not isotype control prior to immunization with rat RBCs increased the incidence of AIHA from 30% to 90%. Adoptive transfer of purified splenic population of CD4(+)CD25(+) but not CD4(+)CD25(-) cells from immunized mice into naive recipients prevented the induction of autoantibody production. Altogether, our data establish a critical role for CD4(+)CD25(+) cells for control of AIHA, which may help to establish therapeutic strategies for treatment of AIHA.
authors.affiliation: Complement Biology, New York Blood Center, 310 E 67th St, New York, NY 10021, USA.
authors.forename: Amina
authors.initials: A
authors.lastname: Mqadmi
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zheng
authors.forename: Karina
authors.initials: K
authors.lastname: Yazdanbakhsh
mesh_terms: D019264:Adoptive Transfer; D000744:Anemia, Hemolytic, Autoimmune / Q000209:etiology* / Q000276:immunology / Q000517:prevention & control; D000818:Animals; D000917:Antibody Formation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D017707:Erythrocyte Transfusion; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D015375:Receptors, Interleukin-2
chemical_list: D015375:Receptors, Interleukin-2