[Basophil activation tests in the diagnosis of drug reactions].
Over the past 10 years a number of studies on basophil activation tests (BAT) have been published which focus on their use as diagnostic methods in clarifying IgE-mediated hypersensitivity reactions to various allergens including drugs. These BAT are based on flow cytometric quantification of allergen- induced CD63 or CD203 expression or measurement of sulfo leukotriene release by ELISA. In the diagnosis of penicillin allergy, the combined use of the CD63-BAT and specific IgE increases sensitivity. The role of BAT in the diagnosis of non-IgE-mediated reactions to non-steroidal anti- inflammatory drugs remains to be clarified. This article focuses on (1) the principles of BAT, (2) technical aspects, (3) clinical applications and (4) recommendations for further development.
Erdmann
S M
SM
Klinik für Dermatologie und Allergologie, Universitätsklinikum Aachen. serdmann@ukaachen.de
Ventocilla
S
S
Moll-Slodowy
S
S
Sauer
I
I
Merk
H F
HF
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D015549:Basophil Degranulation Test / Q000379:methods*; D003937:Diagnosis, Differential; D003875:Drug Eruptions / Q000175:diagnosis*; D006801:Humans; D010406:Penicillins / Q000009:adverse effects*; D017410:Practice Guidelines as Topic; D010818:Practice Patterns, Physicians'; D018570:Risk Assessment / Q000379:methods; D012307:Risk Factors; D012720:Severity of Illness Index
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010406:Penicillins