Chromatographic detection of residual cellular DNA on short monolithic columns.
Analysis of crude samples from biotechnological processes is often required to demonstrate that residual host cell impurities are reduced or eliminated during purification. Current knowledge suggests that a continuous-cell-line DNA can be considered a cellular contaminant rather than a significant risk factor requiring removal to extremely low levels. Anion-exchange chromatography is one of the most important methods used in the downstream processing and analysis of different biomolecules. In this article, an application using Convective Interaction Media monolithic columns to improve the detection of residual cellular DNA is described.
Forcic
Dubravko
D
Institute of Immunology Inc., Rockefeller Street 10, 10000 Zagreb, Croatia. dforcic@imz.hr
Cakanic
Karmen Branovic
KB
Ivancic
Jelena
J
Jug
Renata
R
Barut
Milos
M
Strancar
Ales
A
Mazuran
Renata
R
D002460:Cell Line; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D015894:Genome, Human; D006801:Humans; D008094:Lithium / Q000737:chemistry; D008459:Measles virus / Q000737:chemistry
D004247:DNA; D008094:Lithium