[Study of dual rearrangements of lymphocytic antigen receptor genes in non-Hodgkin lymphoma].
OBJECTIVE
To investigate the rate of dual rearrangements of lymphocytic antigen receptor genes in non-Hodgkin lymphomas (NHL) and its pathogenesis and pathologic significance.


METHODS
PCR analysis of monoclonal, polyclonal and dual rearrangements of IgH and TCR gamma, TCR beta genes was carried out in 125 cases of NHL to evaluate the rate of dual rearrangements, immunohistochemistry was performed for a Ki67 protein expression in 117 cases and the proliferation index was calculated. The relationship between antigen receptor gene rearrangements and proliferation index was analyzed.


RESULTS
Combination of the two pairs of IgH gene primers with the multiplex PCR for TCR gamma and TCR beta gene revealed dual rearrangements in 8% (8/96) of B-NHL, 17% (5/29) of T-NHL. In B cell NHL, IgH gene monoclonal, dural and polyclonal rearrangements were identified in 65, 8 and 15 cases respectively, while in T-cell NHL, they were in 15, 5 and 9 cases, respectively. There was no significant difference between proliferation index and monoclonal, dual, polyclonal rearrangements in both B-NHL and T-NHL by One-way test.


CONCLUSIONS
Dual rearrangements in NHL are not rare and have no relationship with proliferation index.
Zhang
Xiao-fei
XF
Institute of Pathology & Forensic Medicine and Research Center of Environmental Genomics, Zhejiang University, Hangzhou 310006, China.
Zhou
Ren
R
Yang
You-ping
YP
Mao
Zheng-rong
ZR
Yang
Shui-you
SY
Zhang
Suo-jiang
SJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002352:Carrier Proteins / Q000235:genetics; D049109:Cell Proliferation; D002648:Child; D005260:Female; D015321:Gene Rearrangement; D019675:Genes, T-Cell Receptor gamma / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011946:Receptors, Antigen / Q000235:genetics*; D055815:Young Adult
D002352:Carrier Proteins; D019394:Ki-67 Antigen; D011946:Receptors, Antigen