title: Ehrlichia canis in dogs in Yucatan, Mexico: seroprevalence, prevalence of infection and associated factors.
abstract: The aim of this study was to determine the prevalence of infection, seroprevalence, and factors associated with antibody response to Ehrlichia canis in dogs of Yucatan, Mexico. The study was carried out in four veterinary clinics located in Merida, the capital city of Yucatan, Mexico. Blood samples were obtained from a total of 120 dogs, and each animal was physically examined to determine age and sex, as well as to record any clinical signs of platelet-related bleeding. Blood samples were analyzed for antibodies to E. canis using an ELISA test, and thrombocyte counts were calculated. Blood smears were prepared to detect typical morulae in leukocytes. The prevalence of infection, seroprevalence and associated factors were calculated. A primary screening was performed using 2 x K contingency tables of exposure variables. All variables with P< or =0.20 were analyzed by a logistic-binomial regression. Fifty-three (44.1%) of the 120 dogs were found to be seropositive to E. canis. In six dogs (5.0%) typical morulae of E. canis were observed in monocytes. These six cases were positive for antibodies and were thrombocytopenic. The following factors associated with seropositive animals were: platelet-related bleeding (Yes: OR=10.26, CI=2.50-42.16, P=0.001), thrombocytopenia (Yes: OR=18.91, CI=4.47-80.03, P=0.000) and age (2-4 years: OR=6.77, CI=1.76-25.97, P=0.005; >4 years: OR=4.24, CI=1.04-17.21, P=0.043).
authors.affiliation: Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Yucatan, Km 15.5, Carretera Mérida-Xmatkuil, Mérida, CP 97100 Yucatán, México. rvivas@tunku.uady.mx
authors.forename: R I
authors.initials: RI
authors.lastname: Rodriguez-Vivas
authors.forename: R E F
authors.initials: RE
authors.lastname: Albornoz
authors.forename: G M E
authors.initials: GM
authors.lastname: Bolio
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004283:Dog Diseases / Q000097:blood / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs; D041061:Ehrlichia canis / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000097:blood / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008297:Male; D008800:Mexico / Q000453:epidemiology; D010976:Platelet Count / Q000662:veterinary; D015995:Prevalence; D011693:Purpura / Q000662:veterinary; D016036:Seroepidemiologic Studies; D013921:Thrombocytopenia / Q000662:veterinary
chemical_list: D000913:Antibodies, Protozoan