[Qualitative analysis of batch preparing cryopreserved fresh platelet rich plasma].
To evaluate the efficiency and effectiveness of batch preparing cryopreserved fresh platelet-rich plasma (cryo-FPRP) derived from the volunteer donors, platelet count (Plt), mean platelet volume (MPV), platelet distribution width (PDW), plasma pH, plasma lactic acid concentration, and lactic dehydrogenase (LDH) concentration, germiculture, CD62p positive rate, PAC-1 positive rate, and the fluorescence intensity of platelet GPIb-IX-V were detected in ACD whole blood, fresh platelet-rich plasma (FPRP), FPRP with 5% dimethyl sulphoxide DMSO (DMSO-FPRP), and thawed cryopreserved FPRP (cryo-FPRP); the procoagulant activity of FPRP and cryo-FPR was determinated. The results showed that (1) 70 percentage of platelet were separated from the whole blood into FPRP, and the counts of residual erythrocyte and leucocyte were below 1 x 10(9), and below 1 x 10(7) per unit respectively. (2) The plasma pH, lactic acid concentration and PAC-1 positive rate retained a stable level during the preparing, storing and thawing process. (3) Plasma LDH concentration, platelet CD62p positive rate and GPIb-IX-V concentration in platelet surface were enhanced significantly after being frozen and thawing. (4) The plasma clotting time induced by cryo-FPRP were significantly shorter than that induced by FPRP. It is concluded that: (1) The batch platelet preparing process can efficiently obtain platelet from whole blood donated by volunteer, and the process didn't activate the platelet. (2) Cryopreservation can prevent lactic acid accumulation, pH reduce and activation of GPIIb/IIIa. (3) The membrane of partial platelets are affected by freezing and thawing. (4) The density of GPIb-IX-V complexes in platelet surface and its procoagulant activity are enhanced significantly after the FPRP freezing and thawing process.
Ouyang
Xi-Lin
XL
Department of Blood Transfusion, General Hospital of PLA, Centre for Clinic Blood Transfusion of PLA, Beijing 100853, China. ouyang70@301hospital.com.cn
Liu
Jing-Han
JH
Pan
Ji-Chun
JC
Li
Xi-Jin
XJ
Ma
Shu-Xuan
SX
Wang
Hai-Bao
HB
Lai
Feng-Lei
FL
Gao
Da-Yong
DY
D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D019040:E-Selectin / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D015203:Reproducibility of Results
D019040:E-Selectin; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase