Simple and reliable detection of slime production of Candida spp. directly from blood culture bottles: comparison of visual tube method and transmission electron microscopy.
Early detection of slime production may be useful for clinical decision because of its suggestive property for potential pathogenic capacity of a Candida strain especially in patients with a prosthetic device. In this study we aimed to compare the visual tube method (VTM) with transmission electron microscopy (TEM) in order to confirm the reliability of the former method. In order to demonstrate the reproducibility of the tube method and to determine the correct timing for the test, Candida isolates directly obtained from blood culture (DBC) bottles and their two subsequent subcultures were used. The results of this study showed that VTM is a simple and reliable method which can be used in every clinical mycology laboratory, provided that the test is applied on DBC isolates; as the ability of slime production is decreased or lost even after the first subculturing. We suggest that this simple method can be used and may have some contributions to the ongoing studies on the controversial issue concerning removal of biomaterials in candidemic patients.
Cerikcioglu
Nilgun
N
School of Medicine, Department of Microbiology, Marmara University, 34668 Haydarpasa Campus, Istanbul, Turkey. nilguncerik@yahoo.com
Hasdemir
Ufuk Over
UO
San
Tangul
T
Salik
Emsal
E
Soyletir
Guner
G
D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000302:isolation & purification* / Q000648:ultrastructure; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D003470:Culture Media; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009172:Mycology / Q000379:methods* / Q000592:standards
D003470:Culture Media