title: Gene expression profiling in porcine fetal thymus.
abstract: To obtain an initial overview of gene diversity and expression pattern in porcine thymus, 11,712 ESTs (Expressed Sequence Tags) from 100-day-old porcine thymus (FTY) were sequenced and 7,071 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 959 contigs and 3,074 singlets were obtained. Blast search showed that 806 contigs and 1,669 singlets (totally 5,442 ESTs) had homologues in GenBank and 1,629 ESTs were novel. According to the Gene Ontology classification, 36.99% ESTs were cataloged into the gene expression group, indicating that although the functional gene (18.78% in defense group) of thymus is expressed in a certain degree, the 100-day-old porcine thymus still exists in a developmental stage. Comparative analysis showed that the gene expression pattern of the 100-day-old porcine thymus is similar to that of the human infant thymus.
authors.affiliation: The State Laboratory of the Ministry of Health for Forensic Science, Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yanjiong
authors.initials: Y
authors.lastname: Chen
authors.forename: Shengbin
authors.initials: S
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Ye
authors.forename: Jianing
authors.initials: J
authors.lastname: Geng
authors.forename: Yajun
authors.initials: Y
authors.lastname: Deng
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
mesh_terms: D000818:Animals; D019295:Computational Biology; D020224:Expressed Sequence Tags; D005333:Fetus / Q000378:metabolism*; D020869:Gene Expression Profiling; D014644:Genetic Variation; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism*