The inactivation of phospholipase A2 by scalaradial: a biomimetic study by electrospray mass spectrometry.
A biomimetic approach was employed to shed light on the nature of chemical reactions occurring in the covalent inactivation of phospholipase A(2) (PLA(2)) by scalaradial (1), a marine dialdehyde terpenoid endowed with potent anti-inflammatory activity. To this end, a detailed study of the reaction profile between the nitrogenous nucleophile isopropylamine and scalaradial was performed under biologically relevant conditions.
Monti
Maria Chiara
MC
Dipartimento di Scienze Farmaceutiche and Centro Regionale di Competenza in Diagnostica e Farmaceutica Molecolari, Università di Salerno, via Ponte Don Melillo, 84084, Fisciano (SA), Italy.
Casapullo
Agostino
A
Riccio
Raffaele
R
Gomez-Paloma
Luigi
L
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006718:Homosteroids / Q000737:chemistry*; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D011437:Propylamines / Q000737:chemistry*; D054830:Sesterterpenes; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013729:Terpenes / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006718:Homosteroids; D011437:Propylamines; D054830:Sesterterpenes; D013729:Terpenes; C071197:scalaradial; D010741:Phospholipases A; D054467:Phospholipases A2; C035263:2-propylamine