High temperature liquid chromatography of triazole fungicides on polybutadiene-coated zirconia stationary phase.
High temperature liquid chromatography using water-rich and superheated water eluent is evaluated as a new approach for the separation of selected triazole fungicides, hexaconazole, tebuconazole, propiconazole, and difenoconazole. Using a polybutadiene-coated zirconia column at temperatures of 100-150 degrees C, clear separations were achieved when 100% purified water was utilized as organic-free eluent. Excellent limits of detection down to pg level were obtained for the separation of the triazole fungicides under optimum conditions. Van't Hoff plots for the separations were linear suggesting that no changes occurred in the retention mechanism over the temperature range studied.
Sanagi
M Marsin
MM
Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. marsin@kimia.fs.utm.my
See
H H
HH
Ibrahim
Wan Aini Wan
WA
Naim
Ahmedy Abu
AA
D002070:Butadienes / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D020724:Elastomers; D005659:Fungicides, Industrial / Q000032:analysis*; D006358:Hot Temperature; D011108:Polymers / Q000737:chemistry*; D013816:Thermodynamics; D014230:Triazoles / Q000032:analysis*; D015040:Zirconium / Q000737:chemistry*
D002070:Butadienes; D020724:Elastomers; D005659:Fungicides, Industrial; D011108:Polymers; D014230:Triazoles; C028834:polybutadiene; D015040:Zirconium; C028541:zirconium oxide