Effect of colloidal gold size on the conformational changes of adsorbed cytochrome c: probing by circular dichroism, UV-visible, and infrared spectroscopy.
The conformational changes of bovine heart cytochrome c (cyt c) induced by the adsorption on gold nanoparticles with different sizes have been investigated by electronic absorption, circular dichroism (CD), and Fourier transform infrared spectra. The combination of these techniques can give complementary information about adsorption-induced conformational changes. The results show that there are different conformational changes for cyt c adsorbed on gold nanoparticles with different sizes due to the different interaction forces between cyt c and gold nanoparticles. The colloidal gold concentration-dependent conformation distribution curves of cyt c obtained by analysis of CD spectra using the singular value decomposition least-squares method show that the coverage of cyt c on the gold nanoparticles surface also affects the conformational changes of the adsorbed cyt c.
Jiang
Xiue
X
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 China.
Jiang
Junguang
J
Jin
Yongdong
Y
Wang
Erkang
E
Dong
Shaojun
S
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D003102:Colloids / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D049329:Nanostructures; D010316:Particle Size; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D003102:Colloids; D015335:Molecular Probes; D006046:Gold; D045304:Cytochromes c