title: Susceptibility of different eukaryotic cell lines to SARS-coronavirus.
abstract: In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.
authors.affiliation: Robert Koch-Institut, Center for Biological Safety 1, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Hattermann
authors.forename: M A
authors.initials: MA
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Nitsche
authors.forename: S
authors.initials: S
authors.lastname: Wendt
authors.forename: O
authors.initials: O
authors.lastname: Donoso Mantke
authors.forename: M
authors.initials: M
authors.lastname: Niedrig
mesh_terms: D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism; D002415:Cats; D002460:Cell Line; D002522:Chlorocebus aethiops; D005057:Eukaryotic Cells / Q000821:virology*; D006801:Humans; D051379:Mice; D007703:Peptidyl-Dipeptidase A; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D013552:Swine; D014709:Vero Cells
chemical_list: D011991:Receptors, Virus; D002268:Carboxypeptidases; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; C000705328:Ace2 protein, mouse; D000085962:Angiotensin-Converting Enzyme 2