Expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis.
OBJECTIVE
To determine the expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis and its clinical significance.


METHODS
A cervical spondylosis model was established in rats by destroying the stability of cervical posterior column, and the cord segments C(4-6) and gastric antrum were collected 3, 4 and 5 mo after the operation. Rats with sham operation were used as controls. c-fos neuronal counter-staining was performed with an immunohistochemistry method. Every third sections from C(4-6) segments were drawn. The 10 most labeled c-fos-immunoreactive (Fos-IR) neurons were counted, and the average number was used for statistical analysis. The mean of Fos-IR neurons in myenteric plexus was calculated after counting Fos-IR neurons in 25 ganglia from each antral preparation, and expressed as a mean count per myenteric ganglion.


RESULTS
There were a few c-fos-positive neurons in the cervical cord and antrum in the control group. There was an increased c-fos expression in model group 3, 4 and 5 mo after operation, whereas there was no significant increase in c-fos expression in the control group at 3, 4 and 5 mo. More importantly, there was a significant difference in c-fos expression between rats followed up for 3 mo and those for 5 mo in the model group (11.20+/-2.26 vs 27.68+/-4.36, P<0.05, for the cervical cord; and 11.3+/-2.3 vs 29.3+/-4.6, P<0.05, for the gastric antrum). There was no significant difference between rats followed up for 3 mo and those for 4 mo and between rats followed up for 4 mo and those for 5 mo in the model group.


CONCLUSIONS
c-fos expression in gastric myenteric plexus was dramatically associated with that in the spinal cord in rats with cervical spondylosis, suggesting that the gastrointestinal function may be affected by cervical spondylosis. If this hypothesis is confirmed by further studies, functional gastrointestinal diseases such as functional dyspepsia and irritable bowel syndrome could be explained by neurogastroenterology.
Song
Pei-Song
PS
Department of Joint and Spine, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong Province, China.
Kong
Kang-Mei
KM
Niu
Chun-Yan
CY
Qi
Wei-Li
WL
Wu
Ling-Fei
LF
Wang
Xin-Jia
XJ
Han
Wei
W
Huang
Keng
K
Chen
Ze-Feng
ZF
D000818:Animals; D002574:Cervical Vertebrae; D005260:Female; D005767:Gastrointestinal Diseases / Q000209:etiology; D008297:Male; D009197:Myenteric Plexus / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D011706:Pyloric Antrum / Q000294:innervation* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*; D013128:Spinal Osteophytosis / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology
D016760:Proto-Oncogene Proteins c-fos