title: Synthesis, characterization and immobilization of Prussian blue nanoparticles. A potential tool for biosensing devices.
abstract: Prussian blue (PB) particles with the size of ca. 5 nm were synthesized and immobilized in a multilayer structure, as a strategy for the potential development of an amperometric transducer for oxidase-enzyme-based biosensors. Multilayer films composed of PB and poly(allylamine hydrochloride) (PAH) were prepared via layer-by-layer (LbL) sequential deposition. The process was carefully monitored by UV-vis spectroscopy and cyclic voltammetry. The increase of the redox current peaks during the layer-by-layer deposition demonstrated that charge propagation within the film occurs. Linear increase of UV-vis absorbance with the number of deposited bilayers indicates that well-organized systems have been elaborated. ITO electrodes coated with PB/PAH films were used successfully for detecting H2O2, sensitivity being dependent on the number of PB/PAH layers.
authors.affiliation: Instituto de Quimica, Universidade de Sao Paulo, C.P. 26077, 05513-970, Sao Paulo (SP), Brazil.
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Fiorito
authors.forename: Vinicius R
authors.initials: VR
authors.lastname: Gonçales
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Ponzio
authors.forename: Susana I Córdoba
authors.initials: SI
authors.lastname: de Torresi
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D005295:Ferrocyanides / Q000138:chemical synthesis* / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D010088:Oxidoreductases; C000170:ferric ferrocyanide