Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells.
Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction - but not peptidoglycan - of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU-1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU-1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU-1 cells in a dose- and time-dependent manner. Furthermore, treatment of the cells with 50 microg/ml and 100 microg/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU-1 cell growth is mainly attributable to the induction of apoptosis.
Kim
Seo Young
SY
Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-741, South Korea.
Lee
Ki Won
KW
Kim
Ji Yeon
JY
Lee
Hyong Joo
HJ
D000230:Adenocarcinoma; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002843:Chromatin / Q000187:drug effects; D003593:Cytoplasm / Q000737:chemistry*; D006801:Humans; D013294:Lactococcus lactis; D013274:Stomach Neoplasms
D000970:Antineoplastic Agents; D002457:Cell Extracts; D002843:Chromatin