title: Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier: in vitro transfection.
abstract: Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca2+ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Hu-Lin
authors.initials: HL
authors.lastname: Jiang
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Cook
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Su-Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Hwan-Jeong
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D005690:Galactose / Q000008:administration & dosage* / Q000378:metabolism; D006367:HeLa Cells; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D011205:Povidone / Q000008:administration & dosage* / Q000737:chemistry; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D048271:Chitosan; D011205:Povidone; D005690:Galactose