Genotoxicity studies on dietary diacylglycerol (DAG) oil.
Dietary diacylglycerol (DAG) oil is an edible oil enriched in DAG (more than 80%). A recent investigation indicated that DAG oil or its components may have beneficial effects on the prevention and management of obesity. We evaluated the genotoxic potential of DAG oil using standard genotoxicity tests. Bacterial reverse mutation assay (Ames test), the chromosomal aberration assay in cultured Chinese hamster lung cells (CHL/IU), and a bone marrow micronucleus assay in ICR CD mice were employed in the present study. In addition we have tested the possibility that genotoxic substances may be formed during cooking, heated DAG oil (HDG) was prepared by batch frying potato slices in the oil at 180 degrees C for 8 h/day for three consecutive days. Therefore, genotoxicity tests were also performed on HDG. Results obtained did not show any genotoxic effect on either unheated DAG oil (UDG) or HDG. We conclude that there are no safety concerns on the genotoxicity of DAG oil under the conditions for normal use.
Kasamatsu
T
T
Safety and Microbial Control Research Center, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan. kasamatsu.toshio@kao.co.jp
Ogura
R
R
Ikeda
N
N
Morita
O
O
Saigo
K
K
Watabe
H
H
Saito
Y
Y
Suzuki
H
H
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects*; D003296:Cooking / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D004041:Dietary Fats / Q000008:administration & dosage / Q000633:toxicity*; D004075:Diglycerides / Q000008:administration & dosage* / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D008168:Lung / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods*; D009765:Obesity / Q000178:diet therapy; D012449:Safety; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D004041:Dietary Fats; D004075:Diglycerides