[Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells].
OBJECTIVE
To explore the transfer and expression of recombinant human stem cell factor (rh-SCF) gene in human hematopoietic stem/progenitor cell.


METHODS
pLXSN-SCF, a recombinant retrovirus vector of soluble human SCF gene, was constructed by recombinant gene technique. The recombinant plasmid was introduced into virus packaging cells psi2 and PA317 by lipofectin-mediated DNA transfection, and PA317/SCF, the recombinant virus producing cell, was obtained by G418 screening [the virus potency was (2.4-8.5) x 10(5) CFU/ml]. Then, human hematopoietic stem/progenitor cells were transfected with PA317/SCF. The transfer and expression of human SCF gene were assayed by PCR, APAAP immunohistochemical staining and chemoluminance-direct ELISA.


CONCLUSIONS
retrovirus-mediated rh-SCF gene was successfully transferred and expressed in human hematopoietic stem/progenitor cells.
Wang
J
J
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
Zhu
Y
Y
Lai
C
C
Xue
S
S
Ma
H
H
Shen
B
B
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D041681:NIH 3T3 Cells; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D019089:Stem Cell Factor / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D019089:Stem Cell Factor