Mycobacterium sp. mutant strain producing 9alpha-hydroxyandrostenedione from sitosterol.
Mycobacterium sp. VKM Ac-1815D and its derivatives with altered resistance to antibacterial agents were able to produce androst-4-ene-3,17-dione (AD) as a major product from sitosterol. In this study, those strains were subjected to subsequent mutagenization by chemical agents and UV irradiation in combination with sitosterol selection pressure. The mutant Mycobacterium sp. 2-4 M was selected, being capable of producing 9alpha-hydroxyandrost-4-ene-3,17-dione (9-OH-AD) as a major product from sitosterol, with a 50% molar yield. Along with 9-OH-AD, both AD and 9alpha-hydroxylated metabolites with a partially degraded side-chain were formed from sitosterol by the mutant strain. The strain was unable to degrade 9-OH-AD, but degraded androsta-1,4-diene-3,17-dione (ADD), thus indicating a deficiency in steroid 1(2)-dehydrogenase and the presence of 9alpha-hydroxylase activity.
Donova
M V
MV
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Postbox 142290, Pushchino, Moscow Region, Russia. donova@ibpm.pushchino.ru
Gulevskaya
S A
SA
Dovbnya
D V
DV
Puntus
I F
IF
D000730:Androstadienes / Q000378:metabolism; D000735:Androstenedione / Q000031:analogs & derivatives* / Q000378:metabolism; D001711:Biotransformation; D015394:Molecular Structure; D016296:Mutagenesis; D009161:Mycobacterium / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D012641:Selection, Genetic; D012855:Sitosterols / Q000378:metabolism*
D000730:Androstadienes; D012855:Sitosterols; C006573:1,4-androstadiene-3,17-dione; D000735:Androstenedione; C076717:9-hydroxy-4-androstene-3,17-dione; C025473:gamma-sitosterol; D010088:Oxidoreductases; C022656:3-oxosteroid delta(1) dehydrogenase