title: Comparison of glycosylation patterns of placental proteins between normal pregnancy and missed abortion.
abstract: Contemporary understanding of missed abortion, as the case of spontaneous abortion where embryo is retained in uterus for four-weeks or more after its death, is very poor. Aiming to improve the level of knowledge about this process, we have compared glycosylation patterns of placental proteins in normal pregnancy and missed abortion. Oligosaccharide branches were detected by Western-blot using SNA, DBA and PHA-E lectins. The comparison of samples of the same gestational age enabled identification of changes in protein glycosylation between normal and pathological placentas. Lectin DBA detects in normal placenta the glycoprotein GP 105 during the eleventh week, which is absent in missed abortion. PHA-E identifies GP 71 during fourteenth week only in normal placenta. However GP 25 recognized by SNA in missed abortion was not found in normal pregnancy at tenth week. These results indicate that abnormal placental development is associated with changes in glycoprotein structures, and that glycoconjugates might have an important role in placental development.
authors.affiliation: Department of Biology, School of Medicine, University of Zagreb, Zagreb, Croatia. sermanl@mef.hr
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Serman
authors.forename: Alan
authors.initials: A
authors.lastname: Serman
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
authors.forename: Astrid
authors.initials: A
authors.lastname: Milić
authors.forename: Visnja
authors.initials: V
authors.lastname: Latin
authors.forename: Anastazija
authors.initials: A
authors.lastname: Aleksandrova
authors.forename: Drasko
authors.initials: D
authors.lastname: Serman
mesh_terms: D000030:Abortion, Missed / Q000503:physiopathology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D037102:Lectins; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D037102:Lectins; D011257:Pregnancy Proteins