Physical mapping of the pink-eyed dilution complex in mouse chromosome 7 shows that Atp10c is the only transcript between Gabrb3 and Ube3a.
Phenotypic analyses of a set of homozygous-lethal deletion mutants at the pink-eyed dilution (p) locus has resulted in the identification of p-linked obesity locus 1 (plo 1), distal to the p locus, as a locus involved in the modulation of body fat and/or affecting lipid metabolism in these mice. The plo 1 region maps to mouse chromosome 7 (MMU 7) between two genes, Gabrb3 and Ube3a, which have been used as anchor points to generate an integrated deletion and physical map of plo 1 that encompasses about 1.2-1.3 Mb. A deletion/physical map was constructed and the genomic DNA between the two loci was sequenced to identify genes mapping to this region. Data show that Atp10c, a novel type IV ATPase a putative phospholipid transporter, is the only coding unit in this region of the chromosome.
Dhar
Madhu S
MS
318 Jessie Harris Building, Department of Nutrition, University of Tennessee, 1215 Cumberland Avenue, Knoxville, TN 37996, USA. mdhar@utk.edu
Hauser
Loren J
LJ
Nicholls
Robert D
RD
Johnson
Dabney K
DK
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002872:Chromosome Deletion; D022202:Chromosomes, Artificial, Bacterial; D018244:Chromosomes, Artificial, Yeast; D003433:Crosses, Genetic; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D051379:Mice; D020161:Physical Chromosome Mapping; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C076239:P protein, mouse; C471639:UBE3A protein, human; D044767:Ubiquitin-Protein Ligases; D000251:Adenosine Triphosphatases; C478764:ATP10a protein, mouse