Structure and expression of the ornithine decarboxylase gene of Chlamydomonas reinhardtii.
A cDNA was cloned encoding ornithine decarboxylase (ODC) of the unicellular green alga Chlamydomonas reinhardtii. The polypeptide consists of 396 amino acid residues with 35-37% sequence identity to other eukaryotic ODCs. As indicated by the phylogenetic tree calculated by neighbour joining analysis, the Chlamydomonas ODC has the same evolutionary distances to the ODCs of higher plants and mammalians. The Chlamydomonas ODC gene contains three introns of 222, 133, and 129bp, respectively. As revealed by Northern-blot analyses, expression of the Chlamydomonas ODC gene is neither altered throughout the vegetative cell cycle nor modulated by exogenous polyamines.
Voigt
Jürgen
J
Physiologisch-chemisches Institut der Eberhard-Karls-Universität, Hoppe-Seyler-Strasse 4, D-72076 Tübingen, Germany. juergen.voigt@medizin.uni-leipzig.de
Fausel
Martina
M
Bohley
Peter
P
Adam
Karl-Heinz
KH
Marquardt
Otfried
O
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D048028:DNA, Algal / Q000737:chemistry / Q000302:isolation & purification; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009955:Ornithine Decarboxylase / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D048030:RNA, Algal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D048028:DNA, Algal; D018076:DNA, Complementary; D048030:RNA, Algal; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase