[Detection of rubella antibody by using indirect mix enzyme immunosorbent assay].
Monoclonal antibody has the characteristics of high purity, specificity and strong activity. For this reason, we adopted a monoclonal antibody (R-McAb), against rubella to coat the polystyrene EIA microtitration plate for the first step. Then we followed an indirect enzyme immunoassay to detect rubella immunoglobulin G antibody. We call this method the indirect mix enzyme immunosorbent assay (IMEIA). The 257 serum specimens collected from different human groups tested by this assay (IMEIA) and rubella hemagglutination inhibitiion (HAI) test gave a 100% agreement.
Han
X
X
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050.
Yi
M
M
Li
W
W
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C040901:rubella antibodies