Characterisation of a developmentally regulated amino acid transporter gene from Leishmania amazonensis.
The metabolism of protozoan parasites of the Leishmania genus is strongly based on amino acid consumption, but little is known about amino acid uptake in these organisms. In the present work, we identified a Leishmania amazonensis gene (La-PAT1) encoding a putative amino acid transporter that belongs to the amino acid/auxin permease family, a group of H(+)/amino acid symporters. This single copy gene is upregulated in amastigotes, the life cycle stage found in the mammalian host. La-PAT1 putative orthologous sequences were identified in Leishmania infantum, Leishmania donovani, Leishmania major and Trypanosoma.
Geraldo
Murilo V
MV
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
Silber
Ariel M
AM
Pereira
Claudio A
CA
Uliana
Silvia R B
SR
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D018507:Gene Expression Regulation, Developmental; D007891:Leishmania / Q000235:genetics*; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D026905:Amino Acid Transport Systems; D016054:DNA, Protozoan