[Transgenic soybean obtained with Agrobacterium tumefaciens-mediated transformation of embryonic tip of soybean mature seeds].
Regenerated embryonic tips were inoculated with Agrobacterium tumefaciens strain EHA105, which contains binary vector pCAMBIA2301, and cultured for 20 h. Our results showed that the T-DNA transfer efficiency reached up to 63.3% (Table 1) and the transformation efficiency reached up to 6.4%-12.1% (Table 2). The effect of infection time on T-DNA delivery into soybean embryonic tips was determined (Table 1). We also discuss the effects of days of co-cultivation to transient expression and the effects of different AS concentrations to transient expression of gus gene (Figs. 1,2). These data indicate that the embryonic tip regeneration system can be used for efficient, effective Agrobacterium-mediated transformation.
Liu
Hai-Kun
HK
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Wei
Zhi-Ming
ZM
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D013025:Glycine max / Q000196:embryology* / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*