title: Effect of Boophone disticha on human neutrophils.
abstract: Boophone disticha (Amaryllidaceae) are mainly used in Southern Africa for inflammatory conditions. It is also known for its toxic effects. Because of the putative effects on components of the immune system and inflammatory response the effects of extracts of the bulb of Boophane disticha were investigated on ATP production in isolated human neutrophils. Furthermore, one possible mechanism of Boophone disticha's therapeutic properties might be its inhibition of superoxide release from neutrophils. The effect of the extracts on superoxide production of human neutrophils was also investigated. Aqueous and ethanol extracts of the outer and inner scales of the bulb of Boophone disticha was investigated for their effect on human neutrophils. It was decided to test the dry other scales separately from the fleshy inner scales as the parts are also used separately by traditional healers for different applications. ATP production was significantly decreased by ethanol extracts of the inner scales of the bulb. Superoxide production was significantly inhibited by aqueous extracts of the inner and outer scales of the bulb.
authors.affiliation: Department of Pharmacology, Medical University of Southern Africa, Box 225, MEDUNSA 0204, South Africa.
authors.forename: E W
authors.initials: EW
authors.lastname: Botha
authors.forename: C P
authors.initials: CP
authors.lastname: Kahler
authors.forename: W J
authors.initials: WJ
authors.lastname: du Plooy
authors.forename: S H
authors.initials: SH
authors.lastname: du Plooy
authors.forename: L
authors.initials: L
authors.lastname: Mathibe
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000431:Ethanol; D006801:Humans; D066298:In Vitro Techniques; D019668:Liliaceae; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D035281:Plant Tubers / Q000737:chemistry; D013481:Superoxides / Q000037:antagonists & inhibitors; D013997:Time Factors; D014867:Water
chemical_list: D010936:Plant Extracts; D014867:Water; D013481:Superoxides; D000431:Ethanol; D000255:Adenosine Triphosphate