[Analysis of the transgenic rice plants derived from transformed anther calli].
Rice calli derived from anther culture were used as recipient to transfer a rice blight resistance gene, Xa21, into a japonica rice variety, Taipei 309, via Agrobacterium-mediated transformation. Seven green transgenic plants, including one mixoploid, two haploid, and four diploid plants, were regenerated. PCR, Southern blot, FISH and blight resistance analysis all indicated that Xa21 gene has been integrated into the T0 plant genomes. T1 generations of the four diploid T0 plants were further investigated for resistance segregation. Chi2 test showed that two T1 populations segregated with a ratio of 3:1, indicating that a single copy of Xa21 gene was integrated into the genome, whereas the segregation ratios of the other two T1 populations were non-Mendelian. Therefore, the four diploid transgenic plants should be heterozygous diploids.
Jiang
Su
S
Department of Botanical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 201101, China.
Chen
Cai-Yan
CY
Cheng
Zhu-Kuan
ZK
Cai
Run
R
Zhai
Wen-Xue
WX
Zhu
Li-Huang
LH
D006238:Haploidy; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic