Prevalence of Brucella pinnipediae in healthy hooded seals (Cystophora cristata) from the North Atlantic Ocean and ringed seals (Phoca hispida) from Svalbard.
Investigations for Brucella-infections were conducted in 29 hooded seals (Cystophora cristata) caught between Svalbard and Greenland (North Atlantic Ocean; Greenland Sea) autumn 2002, and from 20 ringed seals (Phoca hispida) caught in Billefjord, Svalbard, spring 2003. All animals were apparently healthy and were caught in their natural habitat. Bacteriology on tissue samples from ringed seals was negative, whereas Brucella sp. were recovered in tissues from 11 of the 29 hooded seals (38%), with the highest tissue prevalence in spleen (9/29) and lung lymph nodes (9/24). Anti-Brucella antibodies were detected in sera from 9 hooded seals (31%) (EDTA-modified Slow Agglutination test of Wright, Rose Bengal test, Complement Fixation Test, and Protein-A ELISA). The bacterial isolates all belonged to the genus Brucella according to classical biotyping and PCR analysis based on Insertion Sequence IS711, and were shown to be typical marine mammal strains, based on the occurrence of an IS711 element downstream of the bp26 gene. Their dependency on CO2 for growth, and the presence of one copy each of the omp2a and omp2b gene finally classified them as Brucella pinnipediae. Furthermore, all the hooded seal isolates showed an A+ M+ agglutination profile, which is different from the profile of reference seal strain 2/94 (harbour seal, Phoca vitulina). Thus, these results indicate that B. pinnipediae may contain different biovars. The present results suggest that infection with B. pinnipediae is enzootic in this population. Since the hooded seal is commercially hunted and consumed in Norway, the pathological impact of such infections and their zoonotic potential should be further addressed.
Tryland
Morten
M
Section of Arctic Veterinary Medicine, Department of Food Safety and Infection Biology, The Norwegian School of Veterinary Science, P.O. Box 6204, NO-9292 Tromsø, Norway. morten.tryland@veths.no
Sørensen
Karen Kristine
KK
Godfroid
Jacques
J
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001267:Atlantic Ocean; D002002:Brucella / Q000235:genetics / Q000254:growth & development*; D002006:Brucellosis / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D003168:Complement Fixation Tests / Q000662:veterinary; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008168:Lung / Q000382:microbiology; D008297:Male; D009664:Norway / Q000453:epidemiology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D046048:Seals, Earless / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology
D000907:Antibodies, Bacterial; D004251:DNA Transposable Elements; D004269:DNA, Bacterial