title: Isolation and identification of lactic acid bacteria with effect of immune protection to Eschericia coli in mice.
abstract: Lactic acid bacteria were isolated from an alcohol fermentation broth, and the activity as a probiotic was examined using pathogenic E. coli. Thirty-six strains exhibiting good growth were isolated in the medium of concentrated mush which was a residue resulted in the alcohol distillation process. One of these strains, Lactobacillus paracasei subsp. paracasei I-5, could be grown in the medium containing 8 vol% ethanol and at 45 degrees C. The characteristics were different from the type strain, L. paracasei subsp. paracasei NBRC 15889. L. paracasei I-5 showed an excellent growth in the concentrated mush, which just diluted two-fold and adjusted the pH. ICR mice were fed with a standard germ-free feed (CMF) and the strain I-5 (7 x 10(9) cells/day) was orally administrated for 11 days prior to the intraperitoneal challenge with pathogenic E. coli Juhl. After the challenge, mice administrated the strain I-5 exhibited a high survival rate and survival extension days (p < 0.01) compared with the control. The results suggested that the strain might enhance the animal resistance against microbial pathogens. Neonatal diarrhea caused by E. coli is a serious disease in calf breeding. The strain might be practically valuable to prevent diarrhea in calves.
authors.affiliation: R & D Center, Alcohol Enterprise Head Office, New Energy and Industrial Technology Development Organization, 5-1, Inagehigashi 4-chome, Inage-ku, Chiba-shi, Chiba, 263-0031, Japan. fujii@jp-alcohol.com
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishida-Fujii
authors.forename: Shingo
authors.initials: S
authors.lastname: Goto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuboki
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Hirano
authors.forename: Michiko
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Michikatsu
authors.initials: M
authors.lastname: Sato
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D004926:Escherichia coli / Q000276:immunology*; D016908:Gram-Positive Bacterial Infections / Q000276:immunology*; D007778:Lactobacillus / Q000254:growth & development* / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR