[Nuclear and cytoplasmic genome analysis of somatic hybrid of Triticum aestivum L. and Leymus chinensis (Trin.) Tzvel].
Intergeneric somatic hybrids were obtained by fusion between protoplasts of Triticum aestivum L. cv. Jinan 177 and Leymus chinensis (Trin.) Tzvel. Protoplasts of L. chinensis were exposed to UV (300 microW/cm(2)) for 30 s, 45 s and 1 min before fusion. The results of morphological and chromosomal observation, isozyme pattern as well as RAPD analysis and the 5S rDNA space sequence analysis showed the hybrid nature of the regenerated colonies of fusion combination T (+) L (UV 30 s). Restriction Fragment Length Po1ymorphism (RFLP) analysis using mitochondrion-specific atp6 and chloroplast-specific rbcL probes showed that most of the organelle genomes of the hybrids originated from L. chinensis and some recombination happened between wheat and L. chinensis. The effects of elimination of the nuclear genome of receptor protoplast on hybrid regeneration and receptor organelle genome elimination are discussed.
Chen
Xiu-Ling
XL
School of Life Sciences, Shandong University, Jinan 250100, China.
Xia
Guang-Min
GM
Chen
Hui-Min
HM
D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018745:Genome, Plant; D006822:Hybrid Cells; D006109:Poaceae / Q000235:genetics* / Q000254:growth & development; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D011995:Recombination, Genetic; D014908:Triticum / Q000235:genetics* / Q000254:growth & development