Effects of endothelin-1 or of its receptor A a selective antagonist, on histological and ultrastructural patterns in experimental acute pancreatitis in rats.
The role of endothelin-1 (ET-1) and of its receptor A (ETA) blockade in oedematous acute pancreatitis (AP) remains unclear. In 40 male Wistar rats with i.p. cerulein-induced AP, lasting 4 hours, ET-1 2x0.5 nmol/kg and 2x1.0 nmol/kg or selective ETA antagonist LU 302146, 10 mg/kg and 20 mg/kg was given i.p. simultaneously with cerulein. Histological and ultrastructural studies of pancreatic specimens were done. ET-1 decreased the inflammatory infiltration, but not the mean scores of necrosis and vacuolization in AP. The ultrastructural damage of acinar cells was less evident after ET-1 than in untreated AP. Selective ETA antagonist slightly aggravated the vacuolization and necrosis of acinar cells and some ultrastructural alterations in AP. In conclusion, ET-1, in contrast to selective ETA antagonist, exerts some protective effect in the early course of oedematous cerulein-induced acute pancreatitis in rats.
Andrzejewska
A
A
Department of Medical Pathomorphology, Medical University of Bialystok, Poland. anna.andrzejewska@poczta.fm
Dlugosz
J W
JW
D000208:Acute Disease; D000818:Animals; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D019332:Endothelin-1 / Q000494:pharmacology*; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D010195:Pancreatitis / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D044022:Receptor, Endothelin A / Q000502:physiology*; D014617:Vacuoles / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure
D019332:Endothelin-1; D044022:Receptor, Endothelin A