title: Effect of leonurine on the activity of creatine kinase.
abstract: The effects of leonurine (1) on the activity of creatine kinase (CK) have been studied. The results show that leonurine inhibits enzyme activity in concentration- and time-dependent manners (at 0.75 and 1.51 mmol from 12 to 72 h). There are two mechanisms for the inhibition process. Compound 1 first acts as a non-competitive inhibitor and then as an irreversible inhibitor. Changes of CK were not found in 10% SDS-PAGE, but the amount of dimeric CK decreased in 10% non-SDS gel. The results suggest that 1 can inhibit CK activity by degrading its dimeric structure.
authors.affiliation: Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China. zwang@tsinghua.edu.cn
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Pei-Lin
authors.initials: PL
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ju
mesh_terms: D003402:Creatine Kinase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D013997:Time Factors
chemical_list: C013587:leonurine; D005707:Gallic Acid; D003402:Creatine Kinase