Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture.
Effects of melatonin, extremely-low-frequency magnetic field (ELF-MF), and their combination on AT478 murine squamous cell carcinoma line were studied. Manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (Cu/ZnSOD), and glutathione peroxidase (GSH-Px) were used as markers of cells antioxidative status, and malondialdehyde (MDA) level was used as a marker of lipid peroxidation. After melatonin treatment, antioxidative enzyme activities were increased and MDA level was decreased. Application of ELF-MF on treated cells caused an increase of both superoxide dismutases activity and MDA level, but influence of ELF-MF on GSH-Px activity was negligible. All enzyme activity in culture medium containing melatonin (10(-3), 10(-4), 10(-5) M) after exposure to ELF-MF were significantly diminished compared to cells treated only with melatonin. Also MDA levels after combined treatment with melatonin and ELF-MF were significantly decreased. Observed changes were statistically significant (p<0.05). These results strongly suggest that ELF-MF attenuates antioxidative actions of melatonin on cellular level.
Zwirska-Korczala
K
K
Department of Physiology, Silesian University School of Medicine, Zabrze, Poland.
Adamczyk-Sowa
M
M
Polaniak
R
R
Sowa
P
P
Birkner
E
E
Drzazga
Z
Z
Brzozowski
T
T
Konturek
S J
SJ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D002583:Uterine Cervical Neoplasms
D000975:Antioxidants; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008550:Melatonin