[Isolation of immunoglobulins by thiophilic adsorption chromatography].
The separation of antibodies is a process of great importance. The present paper deals with the method of thiophilic adsorption chromatography (TAC) based on the thiophilic sorbents. Thiophilic sorbents can interact with the immunoglobulins very selectively. Binding of immunoglobulins is dependent on the high ionic strength of the medium. Sorbed immunoglobulins are released by the ionic strength decay. TAC provides immunoglobulins from the plasma (or other sources) in one step. Purity of immunoglobulins is comparable with results of the other methods. Benefits of TAC are delicacy to immunoglobulins, simple handling and low costs of separation. By means of TAC it is possible to eliminate immunoglobulins from biological materials. Thiophilic sorbents are currently available but the possibilities of their exploitation are practically unknown.
Marecek
F
F
D000327:Adsorption; D002845:Chromatography / Q000379:methods*; D007136:Immunoglobulins / Q000302:isolation & purification*
D007136:Immunoglobulins