title: Effects of lymphocyte isolation and timing of processing on detection of CD127 expression on T cells in human immunodeficiency virus-infected patients.
abstract: Decreases in the detection of CD127 expression on T cells of human immunodeficiency virus-infected patients by flow cytometry can occur by delayed processing or by peripheral blood mononuclear cell isolation and cryopreservation. These observations should be considered in the interpretation of functional studies and the planning of multicenter clinical trials.
authors.affiliation: AIDS Monitoring Laboratory, Clinical Services Program, Science Applications International Corp., Frederick, Maryland, USA.
authors.forename: Jeanette
authors.initials: J
authors.lastname: Higgins
authors.forename: Julia A
authors.initials: JA
authors.lastname: Metcalf
authors.forename: Randy A
authors.initials: RA
authors.lastname: Stevens
authors.forename: Michael
authors.initials: M
authors.lastname: Baseler
authors.forename: Martha C
authors.initials: MC
authors.lastname: Nason
authors.forename: H Clifford
authors.initials: HC
authors.lastname: Lane
authors.forename: Irini
authors.initials: I
authors.lastname: Sereti
mesh_terms: D000328:Adult; D000368:Aged; D016477:Artifacts; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D020395:Receptors, Interleukin-7 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D020395:Receptors, Interleukin-7