title: Identification of another B-cell epitope in the type-specific region of equine herpesvirus 4 glycoprotein G.
abstract: Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNPVYSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNPVYSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. kmaeda@yamaguchi-u.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Fuminori
authors.initials: F
authors.lastname: Mizukoshi
authors.forename: Masataka
authors.initials: M
authors.lastname: Hamano
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kai
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006566:Herpesviridae Infections / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary; D023141:Herpesvirus 4, Equid / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*
chemical_list: D018985:Epitopes, B-Lymphocyte; D014759:Viral Envelope Proteins; C076629:glycoprotein G, Equid herpesvirus 4