Aberrant transcription of FHIT gene in intrahepatic cholangiocellular carcinomas induced by N-nitrosobis(2-oxopropyl)amine in hamsters.
Aberrant transcription of the fragile histidine triad (FHIT) gene was investigated in intrahepatic cholangiocellular carcinomas (ICCs) induced by N-nitrosobis(2-oxopropyl)amine (BOP) in female Syrian golden hamsters. The animals received 70 mg/kg of BOP followed by repeated exposure to an augmentation pressure regimen consisting of a choline-deficient diet combined with DL-ethionine and then L-methionine and administration of 20 mg/kg BOP. A total of 14 ICCs were obtained 10 weeks after the beginning of the experiment and total RNAs were extracted from each for assessment of aberrant transcription of the FHIT gene by reverse transcription-polymerase chain reaction analysis. Aberrant transcripts were detected in four out of 14 ICCs (28.6%), as absence in the regions of nucleotides (nt) -75 to 279, nt -75 to 348 and nt -75 to 447. These results suggest that alteration of the FHIT gene may play a role in a small fraction of ICCs induced by BOP in the hamster.
Kitahashi
Tsukasa
T
Department of Food Science and Nutritional Health, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
Tsujiuchi
Toshifumi
T
Satoh
Kenji
K
Ohtsuki
Kozo
K
Konishi
Yoichi
Y
Tsutsumi
Masahiro
M
D017766:Acid Anhydride Hydrolases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001650:Bile Duct Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism / Q000473:pathology*; D002273:Carcinogens / Q000633:toxicity*; D018281:Cholangiocarcinoma / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D006224:Cricetinae; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008647:Mesocricetus; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009602:Nitrosamines / Q000633:toxicity*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D002273:Carcinogens; D009363:Neoplasm Proteins; D009602:Nitrosamines; D012333:RNA, Messenger; D000099170:Bis(5'-adenosyl)triphosphatase; C013622:nitrosobis(2-oxopropyl)amine