mRNA but not plasmid DNA is efficiently transfected in murine J774A.1 macrophages.
Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as compared to control pulsed macrophages. In contrast, introduction of DNA into J774A.1 cells caused apoptosis without expression of the gene of interest. Our results show that mRNA nucleofection is a new high-speed transfection method for macrophages.
Van De Parre
Tim J L
TJ
Division of Pharmacology, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium. tim.vandeparre@ua.ac.be
Martinet
Wim
W
Schrijvers
Dorien M
DM
Herman
Arnold G
AG
De Meyer
Guido R Y
GR
D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000295:instrumentation*
D012333:RNA, Messenger; D004247:DNA