[Liposome-mediated gene transfer into bone marrow cells and its in vitro expansion].
OBJECTIVE
To explore the efficiency of liposome-mediated gene transfer into bone marrow cells and the stability of its expression.


METHODS
Two marker genes (Neo and Lac Z) mediated by liposome were co-transferred into bone marrow cells. Following G418 screening,the positive cells were enriched and expanded in vitro.


RESULTS
The liposome-mediated gene transfer rate in bone marrow cells was 13.33 +/- 2.68%, and after G418 screening, it was 46.06 +/- 3.47%. The blue colouring rates with X-gal staining of these cells were not significantly different before and after expansion.


CONCLUSIONS
Liposome-mediated gene transfer in bone marrow cells is highly efficient and the expression of the transferred gene is stable.
Lu
H
H
Institute of Hematology, Tongji Medical University, Wuhan 430022.
Zou
P
P
Li
C
C
D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D049109:Cell Proliferation; D005697:Galactosides / Q000378:metabolism; D018014:Gene Transfer Techniques; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000378:metabolism; D008081:Liposomes; D013194:Staining and Labeling
D005697:Galactosides; D005839:Gentamicins; D007211:Indoles; D008081:Liposomes; C010680:antibiotic G 418; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside