The cloning of sequences differentially transcribed during the induction of somatic embryogenesis in cucumber (Cucumis sativus L.).
Somatic embryogenesis in cucumber cell suspension culture is a convenient tool to study differential gene expression, particularly during the early stages of this process. In this study, we used the cucumber somatic embryogenesis system to detect genes that were differentially transcribed during the induction of embryo development. We identified and cloned 120 candidate cDNA fragments from differential display gels. The selected cDNAs were confirmed by reverse northern, and 83 were sequenced. The obtained sequences represent 64 independent transcripts. The search for similarities in the databases gave a significant result in 16 cases. The potential involvement of these sequences in somatic embryogenesis is discussed.
Linkiewicz
Anna
A
Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, 02-787 Warsaw, Poland. filipecki@alpha.sggw.waw.pl
Filipecki
Marcin
M
Tomczak
Anna
A
Grabowska
Agnieszka
A
Malepszy
Stefan
S
D002478:Cells, Cultured; D003001:Cloning, Molecular; D018553:Cucumis sativus / Q000196:embryology* / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant
D018076:DNA, Complementary