title: Vaccination with an inactivated virulent feline immunodeficiency virus engineered to express high levels of Env.
abstract: An inactivated virus vaccine was prepared from a pathogenic isolate of feline immunodeficiency virus containing a mutation that eliminated an endocytic sorting signal in the envelope glycoprotein, increasing its expression on virions. Cats immunized with inactivated preparations of this modified virus exhibited strong titers of antibody to Env by enzyme-linked immunosorbent assay. Evidence of protection following challenge demonstrated the potential of this approach to lentiviral vaccination.
authors.affiliation: Retrovirus Research Laboratory, Institute of Comparative Medicine, University of Glasgow, Bearsden Rd., Glasgow G61 1QH, United Kingdom. m.hosie@vet.gla.ac.uk
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Hosie
authors.forename: Dieter
authors.initials: D
authors.lastname: Klein
authors.forename: James M
authors.initials: JM
authors.lastname: Binley
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Dunsford
authors.forename: Oswald
authors.initials: O
authors.lastname: Jarrett
authors.forename: James C
authors.initials: JC
authors.lastname: Neil
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Knapp
authors.forename: Simone
authors.initials: S
authors.lastname: Giannecchini
authors.forename: Donatella
authors.initials: D
authors.lastname: Matteucci
authors.forename: Mauro
authors.initials: M
authors.lastname: Bendinelli
authors.forename: James A
authors.initials: JA
authors.lastname: Hoxie
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Willett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002371:Cat Diseases / Q000517:prevention & control / Q000821:virology; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000517:prevention & control* / Q000821:virology; D015686:Gene Products, env / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D014611:Vaccination / Q000662:veterinary*; D015164:Vaccines, Inactivated / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015686:Gene Products, env; D015164:Vaccines, Inactivated; D014765:Viral Vaccines