[Studies on fingerprinting of Flos Buddleja by RP-HPLC].
OBJECTIVE
To establish fingerprinting of Flos Buddleja by using RP-HPLC for the quality control.


METHODS
The HPLC condition was as follows: Inertsil ODS-3 C18 analytical column (4.6 mm x 250 mm, 5 microm), gredient eluation with MeCN (0.1% TFA)-H2O (0.1%TFA), flow rate 1.0 mL x min(-1), detection wavelength 254 nm. 10 commercial samples were analyzed to establish a fingerprinting.


RESULTS
Among the obtained fingerprinting, most of the detected peaks were separated effectively. The accuracy, repeatability and stability of this method were satisfied. The RSDs of relative retention time and area of aimed peaks which existed in all samples wereless than 5%. Theresults were in accordance with the request of fingerprinting.


CONCLUSIONS
The established fingerprinting can be used for the quality control of Flos Buddleja.
Han
Peng
P
School of Pharmacy, Peking University, Beijing 100083, China.
Cui
Ya-jun
YJ
Guo
Hong-zhu
HZ
Guo
De-an
DA
D032861:Buddleja / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D035264:Flowers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control