title: Oxidative stress induced by fumonisin B1 in continuous human and rodent neural cell cultures.
abstract: Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium verticillioides, which is a common infectant of corn and other cereal grains. Of concern to human health is also a possible airborne exposure to FB1-producing strains of F. verticillioides, which may grow in moisture-damaged buildings. In this study, we have characterized oxidative stress-related parameters induced by FB1 in three different neural cell lines, human SH-SY5Y neuroblastoma, rat C6 glioblastoma and mouse GT1-7 hypothalamic cells. The cells were exposed to graded doses of FB1 between 0.1 and 100 microM for 0-144 h after which the production of reactive oxygen species (ROS), lipid peroxidation, intracellular glutathione (GSH) levels and cell viability were measured. FB1 caused a dose-dependent increase of ROS production in C6 glioblastoma and GT1-7 hypothalamic cells but was without an effect in SH-SY5Y cells. Decreased GSH levels, increased MDA-formation, indicative of lipid peroxidation and necrotic cell death were observed in all cell lines after incubation with FB1. These findings indicate that FB1 induces oxidative stress in human, rat and mouse neural cell cultures.
authors.affiliation: Department of Industrial Hygiene and Toxicology, Finnish Institute of Occupational Health, Topeliuksenkatu 41 a A, FIN-00250 Helsinki, Finland.
authors.forename: Helene
authors.initials: H
authors.lastname: Stockmann-Juvala
authors.forename: Jouni
authors.initials: J
authors.lastname: Mikkola
authors.forename: Jonne
authors.initials: J
authors.lastname: Naarala
authors.forename: Jarkko
authors.initials: J
authors.lastname: Loikkanen
authors.forename: Eivor
authors.initials: E
authors.lastname: Elovaara
authors.forename: Kai
authors.initials: K
authors.lastname: Savolainen
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D037341:Fumonisins / Q000633:toxicity*; D005909:Glioblastoma; D005978:Glutathione / Q000037:antagonists & inhibitors; D006801:Humans; D007031:Hypothalamus; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009183:Mycotoxins / Q000633:toxicity*; D009447:Neuroblastoma; D009498:Neurotoxins / Q000633:toxicity*; D018384:Oxidative Stress; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D037341:Fumonisins; D009183:Mycotoxins; D009498:Neurotoxins; D017382:Reactive Oxygen Species; C056933:fumonisin B1; D008315:Malondialdehyde; D005978:Glutathione