Dark variants of luminous bacteria whole cell bioluminescent optical fiber sensor to genotoxicants.
A stable dark variant separated from photobacterium phosphoreum (A2) was fixed in agar-gel membrane and immobilized onto an exposed end of a fiber-optic linked with bioluminometer. The variant could emit a luminescent signal in the presence of genotoxic agents, such as Mitomycin C (MC). The performance of this whole-cell optical fiber sensor system was examined as a function of several parameters, including gel probe thickness, bacterial cell density, and diameter of the fiber-optic core and working temperature. An optimal response to a model genotoxicant, Mitomycin C, was achieved with agar-bacterial gel membrane: the thickness of gel membrane was about 5 mm; the cell density of bacteria in gel membrane was about 2.0 x 10(7)/ml; the diameter of fiber-optic core was 5.0 mm; the working temperature was 25 degrees C. Under these optimized conditions, the response time was less than 10 h to Mitomycin C, with a lower detection threshold of 0.1 mg/L.
Sun
Yaliang
Y
Department of Chemistry, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhou
Tiebo
T
Guo
Jianli
J
Li
Yiyong
Y
D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D014644:Genetic Variation; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000235:genetics*; D016685:Mitomycin / Q000494:pharmacology* / Q000633:toxicity; D055100:Optical Fibers; D010776:Photobacterium / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D008164:Luminescent Proteins; D016685:Mitomycin