Abies nephrolepis leaf phenolics prevent the inhibition of gap junction intercellular communication by hydrogen peroxide in rat liver epithelial cells.
Recent reports suggest that carcinogenicity of hydrogen peroxide (H2O2) is implicated in inhibition of gap junction intercellular communication (GJIC), which is a cellular event associated with the tumor promotion. The present study investigated the effect of phenolics (KF) from leaves of Abies nephrolepis (Khingan fir) on inhibition of GJIC by H2O2 in WB-F344 rat liver epithelial cells. The phenolics were extracted from fresh leaves by using 80% aqueous methanol, and were analyzed mainly as catechin derivatives including epigallocatechin gallate (EGCG) and catechin itself. KF and EGCG protected the inhibition of GJIC by H2O2, whereas butylated hydroxytoluene, a commercial antioxidant, had no effect. Our results indicate that KF exhibits potential chemopreventive effects against carcinogenesis, which may be attributable to phenolics such as EGCG.
Lee
Sang Jun
SJ
Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
Lee
Ki Won
KW
Lee
Hyong Joo
HJ
D028202:Abies / Q000737:chemistry*; D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008099:Liver / Q000166:cytology*; D010636:Phenols / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D011916:Rats, Inbred F344
D010636:Phenols; D006861:Hydrogen Peroxide