[Clonning and sequencing of partial gene of hepatitis G virus (HGV) from Nanjing of China].
Partial gene of hepatitis G virus (HGV) was cloned and sequenced from the serum of a patient with post-transfusion hepatitis C in Nanjing of China by reverse transcription-polymerase chain reaction (RT-PCR). The sequence showed 89.09%, 92.12%, 87.27%, 93.94% nucleotide identity over the corresponding region of HGU44402, HGU45966, HGU36380 in America and HGV isolate in Hebei Province of China respectively. Forty patients with post-transfusion hepatitis C and thirty patients with hepatitis non A-E were detected for HGV RNA by RT-PCR, the HGV RNA positive rate were 10.00% and 6.67% respectively.
Xu
J
J
Department of Infectious Disease, The 81st Hospital of PLA, Nanjing, China 210002.
Zhou
Y
Y
Xu
J
J
Ju
J
J
Wang
X
X
Chen
W
W
Wang
H
H
D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular / Q000379:methods; D029407:GB virus C / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid