Localization of chicken muscle FBPase in cardiomyocyte nuclei.
Fructose 1,6-bisphosphatase (FBPase; EC 3.1.3.11) localization in cardiomyocyte nuclei has recently been investigated in mammals [FEBS Lett. 539 (2003) 51]. In this study, nuclear localization of FBPase in the cardiac muscle of the chicken was studied by immunohistochemistry and other methods. A result of the electron microscopic investigation was confirmed by immunoblotting analysis. Using MALDI Q-TOF mass spectrometry and Mascot program, the nuclear FBPase was identified as muscle chicken FBPase. FBPase activity in isolated cardiomyocyte nuclei was 5.9 mU/g. Nuclear FBPase was strongly inhibited by allosteric inhibitor AMP. I(0.5) for AMP was 0.16 microM and was the same as for the purified chicken muscle enzyme.
Zmojdzian
Monika
M
Department of Animal Physiology, Zoological Institute, Wroclaw University, Cybulskiego 30, 50-205 Wroclaw, Poland.
Dziewulska-Szwajkowska
Daria
D
Dzugaj
Andrzej
A
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000495:Allosteric Site; D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D002645:Chickens; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009132:Muscles / Q000201:enzymology*; D032383:Myocytes, Cardiac / Q000201:enzymology* / Q000648:ultrastructure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004791:Enzyme Inhibitors; D000249:Adenosine Monophosphate; D006597:Fructose-Bisphosphatase