Felodipine quantification in human plasma by high-performance liquid chromatography coupled to tandem mass spectrometry.
A rapid, sensitive, robust and specific method was developed for the determination and quantitation of felodipine, in human blood plasma by liquid chromatography coupled with tandem mass spectrometry using nimodipine as internal standard. Felodipine was extracted from 0.5 mL human plasma by use of a liquid/liquid procedure using diethyl ether/hexane (80/20, v/v) as eluent. The method included a chromatographic run of 5 min using a C(18) analytical column (100 mm x 4.6 mm i.d.) and the calibration curve was linear over the range from 0.02 to 10 ng mL(-1) (r(2) > 0.994). The between-run precision, determined as relative standard deviation of replicate quality controls, was 5.7% (0.06 ng mL(-1)), 7.1% (0.6 ng mL(-1)) and 6.8% (7.5 ng mL(-1)). The between-run accuracy was +/- 0.0, 2.1 and 3.1% for the above-mentioned concentrations, respectively.
Migliorança
Luis H
LH
Galeno Research Unit, Latino Coelho St., 1301, Parque Taquaral, 13087-010, Campinas, SP, Brazil.
Barrientos-Astigarraga
Rafael E
RE
Schug
B S
BS
Blume
H H
HH
Pereira
Alberto S
AS
De Nucci
Gilberto
G
D002121:Calcium Channel Blockers / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015736:Felodipine / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002121:Calcium Channel Blockers; D015736:Felodipine