title: Role of beta2-integrins for homing and neovascularization capacity of endothelial progenitor cells.
abstract: The mechanisms of homing of endothelial progenitor cells (EPCs) to sites of ischemia are unclear. Here, we demonstrate that ex vivo-expanded EPCs as well as murine hematopoietic Sca-1+/Lin- progenitor cells express beta2-integrins, which mediate the adhesion of EPCs to endothelial cell monolayers and their chemokine-induced transendothelial migration in vitro. In a murine model of hind limb ischemia, Sca-1+/Lin- hematopoietic progenitor cells from beta2-integrin-deficient mice are less capable of homing to sites of ischemia and of improving neovascularization. Preactivation of the beta2-integrins expressed on EPCs by activating antibodies augments the EPC-induced neovascularization in vivo. These results provide evidence for a novel function of beta2-integrins in postnatal vasculogenesis.
authors.affiliation: Molecular Cardiology, Dept. of Medicine III, University of Frankfurt, 60950 Frankfurt, Germany.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Chavakis
authors.forename: Alexandra
authors.initials: A
authors.lastname: Aicher
authors.forename: Christopher
authors.initials: C
authors.lastname: Heeschen
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Sasaki
authors.forename: Ralf
authors.initials: R
authors.lastname: Kaiser
authors.forename: Naual
authors.initials: N
authors.lastname: El Makhfi
authors.forename: Carmen
authors.initials: C
authors.lastname: Urbich
authors.forename: Thorsten
authors.initials: T
authors.lastname: Peters
authors.forename: Karin
authors.initials: K
authors.lastname: Scharffetter-Kochanek
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Zeiher
authors.forename: Triantafyllos
authors.initials: T
authors.lastname: Chavakis
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dimmeler
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000378:metabolism* / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology; D005434:Flow Cytometry; D006614:Hindlimb / Q000098:blood supply / Q000473:pathology; D006801:Humans; D007511:Ischemia / Q000503:physiopathology*; D007963:Leukocytes, Mononuclear; D051379:Mice; D018919:Neovascularization, Physiologic / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*
chemical_list: D018821:CD18 Antigens