title: Semi-quantitative analysis of cytokine expression in asymptomatic canine leishmaniasis.
abstract: The dog is the main reservoir of Leishmania infantum, the parasite responsible for visceral leishmaniasis in Mediterranean countries. The infection in dogs shows different clinical presentations, from subclinical/asymptomatic to a fully developed disease, depending on the host's immune responses. The Th1/Th2 dichotomy is not clear in the different forms of canine leishmaniasis, since the data available from studies of immunity response in canine leishmaniasis are scarce and fragmented. The present work describes the cytokine expression in peripheral blood mononuclear cells (PBMC) obtained from asymptomatic dogs experimentally infected with L. infantum that present a cellular protective immune response. The results obtained from freshly isolated PBMC showed expressions of TNF-alpha, IL-2, IFN-gamma, IL-10 and IL-18 mRNA, similar to those from non-infected dogs. However, there was almost no expression of IL-4 mRNA detected in the asymptomatic infected dogs compared to the control dogs. Unspecific stimulation with ConA promoted the expression in a greater or lower degree of all the cytokines studied. In vitro stimulation of PBMC with soluble leishmanial antigen (SLA) promoted the expression of IL-2, IFN-gamma, TNF-alpha, IL-18, IL-4, IL-6 and IL-10 mRNA, with the two first being specifically induced. Although both Th1 and Th2 cytokines are produced, cell mediated immunity observed in these L. infantum-infected asymptomatic dogs depended on the preferential expression of Th1 cytokines.
authors.affiliation: WHO Collaborating Centre for Leishmaniasis, Parasitology Service, National Centre of Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Chamizo
authors.forename: Javier
authors.initials: J
authors.lastname: Moreno
authors.forename: Jorge
authors.initials: J
authors.lastname: Alvar
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D007371:Interferon-gamma / Q000235:genetics; D020382:Interleukin-18 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D018314:Leishmania infantum; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000662:veterinary*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016207:Cytokines; D020382:Interleukin-18; D012333:RNA, Messenger; D015847:Interleukin-4; D007371:Interferon-gamma