title: Phosphoglycerate mutase has essential arginyl residues.
abstract: Phosphoglycerate mutase is inactivated by butanedione in borate buffer. Inactivation by 0.13 mM reagent correlates with the modification of one arginyl residue per subunit, and is prevented by either 2, 3-diphosphoglycerate or 3-phosphoglycerate. With 0.50 mM butanedione, inactivation is accompanied by the modification of three arginyl residues per subunit, two of which are protected by the combined presence of cofactor and substrate.
authors.affiliation: Department of Chemistry, College of Wooster, Wooster, Ohio 44691, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Borders
authors.forename: B A
authors.initials: BA
authors.lastname: Wilson
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000494:pharmacology; D001120:Arginine / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis / Q000187:drug effects; D003067:Coenzymes / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology; D005988:Glyceric Acids / Q000494:pharmacology; D010736:Phosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D003067:Coenzymes; D004852:Epoxy Compounds; D005988:Glyceric Acids; D019794:2,3-Diphosphoglycerate; C007366:diepoxybutane; C005156:3-phosphoglycerate; D001120:Arginine; D010736:Phosphoglycerate Mutase