[Analysis of presence and difference of sequence of porcine endogenous retrovirus in DNA genomes from peripheral blood white cells of pig and mRNA in tissues from mini-pigs].
The aim is to investigate biological features of porcine endogenous retrovirus (PERV) of pigs in China and to provide basic parameters for evaluation of biological safety of xenotransplantation from pig to human. In this study, basic biologic features of PERV of DNA of peripheral blood white cells of 12 species of domestic pigs in China were examined by polymerase chain reaction; Analysis of difference of PERV gene sequence was investigated by sliver-stained single stranded conformational polymorphism and restriction fragment length polymorphism; Difference of PERV mRNA from a variety of tissue of two species of mini-pig was analysized by reverse transcriptase-PCR and semi-quantitative analysis. These results showed that Sequences of PERV-A, -B genes are ubiquitous in DNA genome of peripheral blood white cells in the examined pigs; There was no single stranded conformational polymorphism in PERV-A, -B gene sequences. There is the phenomenon of restriction fragment length polymorphism of sequence of PERV env gene in DNA genome of peripheral blood white cells in some pigs of the examined pigs. The PERV-A, -B and -C are expressive in a variety of tissues from two species of mini-pig, higher in kidneys, lymph nodes and livers, lower in pancreas and brains. It is deserved that the phenomenon of restriction fragment length polymorphism of PERV and the absence and mismatch of base of sequence of PERV-A may induce potential hazard of infection of PERV during xenotransplantation.
Ding
Sheng Cai
SC
Research Institute of Pathology, South-West Hospital, Third Military Medical University, Chongqing, 400038, China.
Chen
Yi Sheng
YS
Wei
Hong
H
Shang
Hai Tao
HT
Liu
Ping
P
D000818:Animals; D001483:Base Sequence; D020077:Endogenous Retroviruses / Q000235:genetics*; D016678:Genome / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics* / Q000821:virology; D013556:Swine, Miniature / Q000235:genetics* / Q000821:virology; D014183:Transplantation, Heterologous
D012333:RNA, Messenger