Actinide and metal toxicity to prospective bioremediation bacteria.
Bacteria may be beneficial for alleviating actinide contaminant migration through processes such as bioaccumulation or metal reduction. However, sites with radioactive contamination often contain multiple additional contaminants, including metals and organic chelators. Bacteria-based bioremediation requires that the microorganism functions in the presence of the target contaminant, as well as other contaminants. Here, we evaluate the toxicity of actinides, metals and chelators to two different bacteria proposed for use in radionuclide bioremediation, Deinococcus radiodurans and Pseudomonas putida, and the toxicity of Pu(VI) to Shewanella putrefaciens. Growth of D. radiodurans was inhibited at metal concentrations ranging from 1.8 microM Cd(II) to 32 mM Fe(III). Growth of P. putida was inhibited at metal concentrations ranging from 50 microM Ni(II) to 240 mM Fe(III). Actinides inhibited growth at mM concentrations: chelated Pu(IV), U(VI) and Np(V) inhibit D. radiodurans growth at 5.2, 2.5 and 2.1 mM respectively. Chelated U(VI) inhibits P. putida growth at 1.7 mM, while 3.6 mM chelated Pu(IV) inhibits growth only slightly. Pu(VI) inhibits S. putrefaciens growth at 6 mM. These results indicate that actinide toxicity is primarily chemical (not radiological), and that radiation resistance does not ensure radionuclide tolerance. This study also shows that Pu is less toxic than U and that actinides are less toxic than other types of metals, which suggests that actinide toxicity will not impede bioremediation using naturally occurring bacteria.
Ruggiero
Christy E
CE
Chemistry, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Boukhalfa
Hakim
H
Forsythe
Jennifer H
JH
Lack
Joseph G
JG
Hersman
Larry E
LE
Neu
Mary P
MP
D008671:Actinoid Series Elements / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity*; D001673:Biodegradation, Environmental; D002614:Chelating Agents / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity*; D034301:Deinococcus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods; D011005:Plutonium / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D016958:Pseudomonas putida / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011848:Radioactive Pollutants / Q000378:metabolism; D011868:Radioisotopes / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity*; D020614:Shewanella putrefaciens / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D008671:Actinoid Series Elements; D002614:Chelating Agents; D011848:Radioactive Pollutants; D011868:Radioisotopes; D011005:Plutonium