Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier: in vitro transfection.
Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca2+ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.
Park
In-Kyu
IK
School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
Jiang
Hu-Lin
HL
Cook
Seung-Eun
SE
Cho
Myung-Haing
MH
Kim
Su-Il
SI
Jeong
Hwan-Jeong
HJ
Akaike
Toshihiro
T
Cho
Chong-Su
CS
D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D005690:Galactose / Q000008:administration & dosage* / Q000378:metabolism; D006367:HeLa Cells; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D011205:Povidone / Q000008:administration & dosage* / Q000737:chemistry; D014162:Transfection / Q000379:methods*
D004337:Drug Carriers; D048271:Chitosan; D011205:Povidone; D005690:Galactose