title: In vivo and in vitro tissue-specific expression of green fluorescent protein using the cre-lox system in mouse embryonic stem cells.
abstract: Embryonic stem cells (ES) are pluripotent and may therefore serve as a source for the generation of specific cell types required for future therapies based on cell replacement. The isolation of defined cell populations from a certain lineage or tissue is a prerequisite for the analysis of the potential of such ES-derived cells in animal transplantation studies. Here, using the Cre/loxP system, we report the generation of murine ES cells conditionally expressing the hrGFP gene at the cell surface. Such ES cells can be differentiated in vitro into neurons displaying GFP activity in neurites. Transgenic mice derived from these ES cells permit the targeting of GFP-expression to specific tissues and provide material from the three germ layers suitable for molecular and biochemical analysis.
authors.affiliation: Georg-August University of Göttingen, Department of Clinical Neurophysiology, Göttingen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Schindehütte
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Fukumitsu
authors.forename: Patrick
authors.initials: P
authors.lastname: Collombat
authors.forename: Gundula
authors.initials: G
authors.lastname: Griesel
authors.forename: Christopher
authors.initials: C
authors.lastname: Brink
authors.forename: Paul C
authors.initials: PC
authors.lastname: Baier
authors.forename: Mario R
authors.initials: MR
authors.lastname: Capecchi
authors.forename: Ahmed
authors.initials: A
authors.lastname: Mansouri
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases