[Nucleotide polymorphism in the drought induced transcription factor CBF4 region of Arabidopsis thaliana and its molecular evolution analyses].
Intraspecific nucleotide polymorphism in the drought induced transcription factor CBF4 region of Arabidopsis thaliana was analyzed with 17 core accessions growing in different ecoclimate. High density of single nucleotide polymorphism (SNP) and insertion/deletion (Indel) were found, on average 1 SNP per 35.8 bp and 1 Indel per 143 bp. Nucleotide polymorphism in non-coding region was three times higher than that in coding region. In coding region of CBF4, SNP frequency is one SNP per 96.4 bp, one nonsynonymous mutation was detected from 25 av, 203 av and 244 av accessions, which is the 205th site amino acid variation: gly <--> val caused by the 1034th site (corresponding to 19,696 site nucleotide of GenBank No. AB015478 as 1) nucleotide variation: G <--> T. Statistical result of nucleotide diversity showed that linkage disequilibrium (LD) existed in large-scale region of CBF4 and recombination event was also detected in 5' non-coding region. Identical to the results of other genes of Arabidopsis, different regions of the gene were seemingly under different selective pressures. Balancing selection resulted in high nucleotide diversity in 3' non-coding region, and the neutral mutation hypothesis can explain the DNA polymorphism in coding region, whereas, nature positive selection in the population affected nucleotide variation in 5' non-coding region of gene.
Hao
Gang-Ping
GP
Beijing Agro-Biotechnology Research Center, Beijing 100089, China. haogangping@163.com
Wu
Zhong-Yi
ZY
Cao
Ming-Qing
MQ
Pelletier
Georges
G
Brunel
Dominique
D
Huang
Cong-Lin
CL
Yang
Qing
Q
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D004190:Disasters; D019143:Evolution, Molecular; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D015534:Trans-Activators / Q000235:genetics*
D029681:Arabidopsis Proteins; C470668:CBF4 protein, Arabidopsis; D015534:Trans-Activators