title: Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signalling.
abstract: Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.
authors.affiliation: Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, School of Medicine, Room 225, Emory University, 615 Michael Street, Atlanta, GA 30322, USA.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Halvey
authors.forename: Walter H
authors.initials: WH
authors.lastname: Watson
authors.forename: Jason M
authors.initials: JM
authors.lastname: Hansen
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Go
authors.forename: Afshin
authors.initials: A
authors.lastname: Samali
authors.forename: Dean P
authors.initials: DP
authors.lastname: Jones
mesh_terms: D002451:Cell Compartmentation / Q000502:physiology*; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry / Q000502:physiology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000737:chemistry / Q000502:physiology; D004220:Disulfides / Q000378:metabolism*; D004594:Electrophysiology; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D004220:Disulfides; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; C491203:TXN protein, human; D013879:Thioredoxins; D004815:Epidermal Growth Factor; D066246:ErbB Receptors