title: [Studies of microsatellite isolation from porcine PAC clone].
abstract: A novel microsatellite DNA was isolated from a single porcine PAC clone by sequencing the PAC clone directly with (CA)n repeat motif anchored primer. The specific primer pairs flanking the (CA)n repeat region were used to amplify the genomic DNA of 40 individuals from 8 pig breeds, which detected three alleles with the fragment length of 305 bp, 307 bp and 309 bp. The PCR product sequencing results of homozygous animals representing three alleles revealed that those three alleles contained 12,13 and 14 CA dinucleotide repeats respectively.
authors.affiliation: Jiangxi Provincial Key Laboratory for Animal Biotechnology, JAU, Nanchang, 330045, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Ke-Fei
authors.initials: KF
authors.lastname: Chen
authors.forename: Lu-Sheng
authors.initials: LS
authors.lastname: Huang