title: Pharmacology of stomoxytachykinin receptor depends on second messenger system.
abstract: STKR is a neurokinin receptor derived from the stable fly, Stomoxys calcitrans. Insect tachykinin-related peptides, also referred to as "insectatachykinins", produce dose-dependent calcium and cyclic AMP responses in cultured Drosophila melanogaster Schneider 2 (S2) cells that were stably transfected with the cloned STKR cDNA. Pronounced differences in pharmacology were observed between agonist-induced calcium and cyclic AMP responses. The results indicate that the pharmacological properties of STKR depend on its coupling to a unique second messenger system. Therefore, a model postulating the existence of multiple active receptor conformations is proposed. This article presents the first evidence that an insect peptide receptor with dual coupling properties to second messenger systems can display agonist-dependent functional differences.
authors.affiliation: Laboratory for Developmental Physiology, Genomics and Proteomics, Zoological Institute, Naamsestraat 59, B-3000 Leuven, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Poels
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Nachman
authors.forename: Karl E
authors.initials: KE
authors.lastname: Akerman
authors.forename: Hendrica B
authors.initials: HB
authors.lastname: Oonk
authors.forename: Felix
authors.initials: F
authors.lastname: Guerrero
authors.forename: Arnold
authors.initials: A
authors.lastname: De Loof
authors.forename: Anna E
authors.initials: AE
authors.lastname: Janecka
authors.forename: Herbert
authors.initials: H
authors.lastname: Torfs
authors.forename: Jozef
authors.initials: J
authors.lastname: Vanden Broeck
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D015290:Second Messenger Systems / Q000187:drug effects*; D015320:Tachykinins / Q000494:pharmacology*
chemical_list: D010455:Peptides; D015320:Tachykinins; D000242:Cyclic AMP; D002118:Calcium