[MMP-26 expression in endometrial explants treated with estradiol and progesterone].
OBJECTIVE
To examine possible estrogen dependent endometrial expression of MMP-26 in vitro.


METHODS
Experimental study.


METHODS
Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden.


METHODS
We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferative phase of the menstrual cycle. Samples were cultured for five days either with estradiol alone or in combination with progesterone. Samples cultured with ethanol represented control groups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization. Immunohistochemistry was used to study the presence of estrogen and progesterone receptors in endometrial explants.


RESULTS
MMP-26 mRNA expression was highest in fresh (non cultured) samples. Signal intensity decreased during the first two days of culture and was negligable in the following days. Nuclear intensity for estrogen and progesterone receptor was high after five days of culture.


CONCLUSIONS
We did not find MMP-26 mRNA in vitro expression to be directly estrogen dependent.
Pilka
R
R
Gynekologicko-porodnická klinika, FN a LF UP, Olomouc.
Kudela
M
M
Hansson
S
S
Casslén
B
B
D000328:Adult; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005498:Follicular Phase; D006801:Humans; D066298:In Vitro Techniques; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D053505:Matrix Metalloproteinases, Secreted; D011374:Progesterone / Q000494:pharmacology*
D011374:Progesterone; D004958:Estradiol; C410599:MMP26 protein, human; D020782:Matrix Metalloproteinases; D053505:Matrix Metalloproteinases, Secreted