[Purification of hemagglutinin of hemorrhagic fever with renal syndrome virus by high pressure liquid chromatography and analysis of immunogenicity].
The brains of suckling mice and suspension of Vero-E6 cells inoculated with Chen's strain of hemorrhagic fever with renal syndrome virus (HFRSV) were obtained and prepared into high titre hemagglutinin (HAN). The crude HAN was purified by high pressure liquid chromatography. The molecular weight of the purified HAN is 56600 and has higher titre of hemagglutinin activity. It has been proved that the purified HAN has homogeneous molecular weight and buoyant density through SDS-PAGE and sucrose gradient ultracenrifugation. Nine monoclonal antibodies (McAb) from varions HFRS virus have been used for analyzing the epitope of purified HAN by ELISA. It was demonstrated that the purified HAN has epitopes of hemagglutinin and neutralizing antigens. The Balb/C mice could be induce specific antibody against HFRSV by immunization with purified HAN. The titre of neutralization was 40 and titre of hemagglutinin inhibition was 64. Some problems concerning the HAN of HFRS virus were discussed.
Xue
X
X
Laboratory of Molecular Virology, 4th Military Medical University, Xian 710032.
Wang
M
M
Xu
Z
Z
Ma
W
W
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D018063:Hantaan virus / Q000378:metabolism*; D006388:Hemagglutinins / Q000276:immunology* / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C
D006388:Hemagglutinins