title: Role of Ca2+ store in AMPA-triggered Ca2+ dynamics in retinal horizontal cells.
abstract: Fura-2 fluorescent calcium imaging was applied to measure [Ca(2+)](i) in freshly dissociated horizontal cells of carp retina, and a model containing endoplasmic reticulum (ER) membrane processes and plasma membrane processes was constructed for quantitative analyses of the AMPA-triggered calcium dynamics. A transient increase followed by a sustained steady level of [Ca(2+)](i) was observed when 100 microM AMPA was applied, while the initial transient increase of [Ca(2+)](i) was suppressed by exogenously applied ryanodine. The model analyses results suggest that the AMPA-triggered calcium dynamics involves a number of cytoplasmic and endoplasmic processes that interact with each other. It also suggests that calcium store is an important part contributing to the transient calcium signal.
authors.affiliation: Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, USA.
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Pei-Ji
authors.initials: PJ
authors.lastname: Liang
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001581:Benzothiadiazines / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D002478:Cells, Cultured; D004347:Drug Interactions; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D016257:Fura-2 / Q000378:metabolism; D006054:Goldfish; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism*; D017711:Nonlinear Dynamics; D012160:Retina / Q000166:cytology*; D012433:Ryanodine / Q000494:pharmacology; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology*
chemical_list: D000959:Antihypertensive Agents; D001581:Benzothiadiazines; D018690:Excitatory Amino Acid Agonists; D012433:Ryanodine; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C004639:cyclothiazide; D002118:Calcium; D016257:Fura-2