[The role of MPO mRNA, IgH mRNA and CD3epsilon mRNA expressions in classification of acute leukemias].
OBJECTIVE
To compare the role of genotypic and immunophenotypic markers in classification of acute leukemia.


METHODS
Expression of myeloperoxidase (MPO), CD3epsilon chain and immunoglobulin heavy chain (IgH) gene in 69 cases of acute leukemia were studied by in situ hybridization with streptavidin-gold (ISH-SAG).


CONCLUSIONS
MPO mRNA and CD3epsilon mRNA expressions were specific for early diagnosis of AML and T-ALL, respectively; and IgH mRNA expression did for early B cell leukemia. Combination of genotypic and immunophenotypic markers assured accurate lineage diagnosis of leukemias.
Ai
H
H
North Tai Ping Road Hospital, Beijing 100039.
Zhu
Y
Y
Shen
B
B
Lai
C
C
Pian
H
H
Jia
S
S
D017252:CD3 Complex / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000235:genetics*; D007938:Leukemia / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D008297:Male; D009195:Peroxidase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D017252:CD3 Complex; C115335:CD3E protein, human; D007132:Immunoglobulin Isotypes; D012333:RNA, Messenger; D009195:Peroxidase