title: Intraocular levels of methotrexate after oral low-dose treatment in chronic uveitis.
abstract: OBJECTIVE
  To determine the intraocular levels of methotrexate in low-dose treatment of noninfectious uveitis.
  
  
  METHODS
  One day after oral administration, the methotrexate level was measured in the aqueous humor and serum of a patient with noninfectious uveitis, who underwent cataract surgery. A fluorescence polarization immunoassay was used for determination.
  
  
  RESULTS
  After oral administration, methotrexate was only measurable in aqueous humor but not in serum.
  
  
  CONCLUSIONS
  In uveitis, orally administered low-dose methotrexate reaches detectable levels in aqueous humor, even in the absence of detectable levels in serum.
authors.affiliation: Department of Ophthalmology, Johann Wolfgang Goethe University, Frankfurt/Main, Germany. j.puchta@gmx.de
authors.forename: Joachim
authors.initials: J
authors.lastname: Puchta
authors.forename: Lars-Olof
authors.initials: LO
authors.lastname: Hattenbach
authors.forename: Holger
authors.initials: H
authors.lastname: Baatz
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D001082:Aqueous Humor / Q000378:metabolism*; D001682:Biological Availability; D002908:Chronic Disease; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008727:Methotrexate / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014605:Uveitis / Q000188:drug therapy* / Q000378:metabolism*
chemical_list: D007166:Immunosuppressive Agents; D008727:Methotrexate