Modulation of haematopoetic system and antioxidant enzymes by Emblica officinalis gaertn and its protective role against gamma-radiation induced damages in mice.
The radio protective effect of the fruit pulp of Emblica officinalis Gaertn (Emblica) was studied in adult Swiss albino mice. Mice were treated with 2.5 g/kg b.wt of Emblica for 10 consecutive days before irradiation and exposed to a single dose of 700 rads (7Gy) of radiation after the last dose. One group was given Emblica continuously for another 15 days after irradiation. Changes in the total leukocyte count, bone marrow viability and hemoglobin were studied after whole body irradiation. Administration of Emblica significantly increased these levels, which were lowered by irradiation. Animals were sacrificed at various time points after irradiation and the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione-S-transferase (GST), and levels of glutathione were assayed in the blood. The damage to the cell membrane after whole body irradiation was studied by measuring the tissue lipid peroxides levels. Administration of Emblica significantly enhanced the activity of the various antioxidant enzymes and GST as well as glutathione system in the blood. Treatment with Emblica also lowered the elevated levels of lipid peroxides in the serum. The data clearly indicated that the extract significantly reduced the bioeffects of radiation. Emblica extract may be useful in reducing the side effects produced during therapeutic radiation.
Hari Kumar
K B
KB
Amala Cancer Research Centre, Thrissur, Kerala State, India.
Sabu
M C
MC
Lima
P S
PS
Kuttan
Ramadasan
R
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001854:Bone Marrow Cells; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D004249:DNA Damage; D005260:Female; D005720:Gamma Rays; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D006454:Hemoglobins / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D015227:Lipid Peroxidation; D051379:Mice; D008954:Models, Biological; D018384:Oxidative Stress; D029842:Phyllanthus emblica / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D006454:Hemoglobins; D010936:Plant Extracts; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase