High reproducibility of serum anti-Mullerian hormone measurements suggests a multi-staged follicular secretion and strengthens its role in the assessment of ovarian follicular status.
BACKGROUND
Our aim was to compare the intercycle reproducibility of serum anti-Mullerian hormone (AMH) measurements with that of other markers of ovarian follicular status.


METHODS
Forty-seven normo-ovulatory, infertile women underwent serum AMH, inhibin B, estradiol and FSH measurements and early antral follicle (2-12 mm in diameter) counts by transvaginal ultrasound on cycle day 3 during three consecutive menstrual cycles. Reproducibility of measurements was estimated using intra-class correlation coefficient (ICC) calculation. We also assessed the number of replicate measurements theoretically needed to reach satisfactory reliability of results.


RESULTS
Serum AMH showed significantly higher reproducibility (ICC, 0.89; 95% confidence interval, 0.83-0.94) than inhibin B (0.76; 0.66-0.86; P < 0.03), estradiol (0.22; 0.03-0.41; P < 0.0001) and FSH levels (0.55; 0.39-0.71; P < 0.01), and early antral follicle counts (0.73; 0.62-0.84; P < 0.001), and reached satisfactory reliability with a single measurement.


CONCLUSIONS
The improved cycle-to-cycle consistency of AMH as compared with other markers of ovarian follicular status is in keeping with its peculiar production by follicles at several developmental stages and further supports its role as a cost-effective, reliable marker of ovarian fertility potential.
Fanchin
Renato
R
Department of Obstetrics and Gynecology, Clamart, Kremlin-Bicêtre, France. renato.fanchin@abc.ap-hop-paris.fr
Taieb
Joëlle
J
Lozano
Daniel H Mendèz
DH
Ducot
Béatrice
B
Frydman
René
R
Bouyer
Jean
J
D000328:Adult; D000375:Aging / Q000502:physiology; D054304:Anti-Mullerian Hormone; D015415:Biomarkers / Q000097:blood; D002624:Chemistry, Clinical / Q000592:standards; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006023:Glycoproteins / Q000097:blood* / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000097:blood* / Q000175:diagnosis*; D007265:Inhibins / Q000097:blood; D008597:Menstrual Cycle; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D011446:Prospective Studies; D015203:Reproducibility of Results; D013735:Testicular Hormones / Q000097:blood* / Q000378:metabolism*
D015415:Biomarkers; D006023:Glycoproteins; D013735:Testicular Hormones; C422364:inhibin B; D004958:Estradiol; D007265:Inhibins; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone