Evaluation of a West Nile virus immunoglobulin A capture enzyme-linked immunosorbent assay.
An in-house-developed enzyme-linked immunosorbent assay detected West Nile virus (WNV) immunoglobulin A (IgA) in 65 of 68 sera from WNV-infected patients; 40 of 63 WNV IgM-positive, IgG-negative serum or plasma specimens; 65 of 67 WNV IgM-positive, IgG-positive specimens; 0 of 70 WNV IgM-negative, IgG-negative specimens; and 0 of 64 archived blood donation sera. WNV IgA is thus highly prevalent among WNV-infected patients and typically appears after WNV IgM but before WNV IgG.
Prince
Harry E
HE
Focus Technologies, 5785 Corporate Ave., Cypress, CA 90630, USA. hprince@focustechnologies.com
Lapé-Nixon
Mary
M
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014901:West Nile Fever / Q000097:blood / Q000175:diagnosis*; D014902:West Nile virus / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M