[Overexpression of GST gene accelerates the growth of transgenic Arabidopsis under salt stress].
The Suaeda salsa glutathione s-transferase gene (GST) was inserted downstream of the 35S promoter in the plant expression vector pROK II and then was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens through floral dip method. Transformants were selected for their ability to grow on medium containing kanamycin. The fact that the GST gene had been transferred into the Arabidopsis thaliana genome was confirmed by the PCR-Southern blotting analysis. After cultivation, independent homozygous transgenic lines were obtained after selection of T(3) progenies on MS medium containing kanamycin. The expression of the gene transferred into the Arabidopsis thaliana was confirmed by Northern blotting. During salt stress, analysis of total glutathione (both oxidized and reduced type) and biomass of transgenic and wild Arabidopsis. The biomass of transgenic lines (GT) was slightly but significantly greater than that of wild type line (WT), and levels of oxidized glutathione (GSSG) were significantly higher in transgenic lines than in wild type. Therefore, overexpression of GST can increase Arabidopsis growth under salt stress, and this effect can be caused by oxidation of the reduced glutathione (GSH ).
Qi
Yuan-Cheng
YC
College of Biological Technique and Food Science, Henan Agricultural University, Zhengzhou 450002, China. yuanchengqi2003@163.com
Zhang
Shi-Min
SM
Wang
Li-Ping
LP
Wang
Ming-Dao
MD
Zhang
Hui
H
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005978:Glutathione / Q000032:analysis; D019803:Glutathione Disulfide / Q000032:analysis; D005982:Glutathione Transferase / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000032:analysis; D012965:Sodium Chloride / Q000494:pharmacology*
D012333:RNA, Messenger; D012965:Sodium Chloride; D005982:Glutathione Transferase; D005978:Glutathione; D019803:Glutathione Disulfide