Ship-in-a-bottle synthesis of fluorescence-labeled nanoparticles: applications in cellular imaging.
Fluorescein (2-(6-hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid) has been prepared inside the pores of zeolite-Y via ship-in-a-bottle synthesis. Fluorescein, whose dimensions prevent it from entering through the approximately 7 A windows of the faujasite zeolite used, was prepared by the acid-catalyzed reaction of resorcinol and phthalic anhydride. In this article we report initial spectroscopic data as well as an example of the usefulness of these fluorescence-labeled nanoparticles for imaging applications such as confocal fluorescence microscopy. Encapsulated fluorescein shows a remarkable increase in photostability.
Chrétien
Michelle N
MN
Center for Catalysis Research and Innovation, Department of Chemistry, University of Ottawa, Ottawa, Canada.
Shen
Biao
B
García
Hermenegildo
H
English
Ann M
AM
Scaiano
J C
JC
D002478:Cells, Cultured; D019793:Fluorescein / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017641:Zeolites / Q000737:chemistry
D005456:Fluorescent Dyes; D017641:Zeolites; D019793:Fluorescein