title: [Amplification of 5' cDNA end of a disease resistance gene homolog using RACE system from soybean].
abstract: Based on part of a known partial cDNA sequence of a disease resistance gene homolog, KR3-1, obtained by screening a cDNA library from soybean, 5'-RACE-PCR was carried out with gene specific primers and universal primers. After the nested PCR reaction,an amplified fragment of 447 bp in length which overlapped the known KR3-1 sequence by 129 bp was obtained subsequently. Thus, a 5' cDNA end of KR3 was successfully cloned.
authors.affiliation: Central Laboratory, Southwest Agricultural University, Chongqing 400716, China bjwang2002@yahoo.com.cn
authors.forename: Bang-Jun
authors.initials: BJ
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhang
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Zhang
authors.forename: Xue-gang
authors.initials: XG
authors.lastname: Li