title: Nonequivalence of the nucleotide binding domains of the ArsA ATPase.
abstract: The arsRDABC operon of Escherichia coli plasmid R773 encodes the ArsAB pump that catalyzes extrusion of the metalloids As(III) and Sb(III), conferring metalloid resistance. The catalytic subunit, ArsA, is an ATPase with two homologous halves, A1 and A2, connected by a short linker. Each half contains a nucleotide binding domain. The overall rate of ATP hydrolysis is slow in the absence of metalloid and is accelerated by metalloid binding. The results of photolabeling of ArsA with the ATP analogue 8-azidoadenosine 5'-[alpha-(32)P]-triphosphate at 4 degrees C indicate that metalloid stimulation correlates with a >10-fold increase in affinity for nucleotide. To investigate the relative contributions of the two nucleotide binding domains to catalysis, a thrombin site was introduced in the linker. This allowed discrimination between incorporation of labeled nucleotides into the two halves of ArsA. The results indicate that both the A1 and A2 nucleotide binding domains bind and hydrolyze trinucleotide, even in the absence of metalloid. Sb(III) increases the affinity of the A1 nucleotide binding domain to a greater extent than the A2 nucleotide binding domain. The ATP analogue labeled with (32)P at the gamma position was used to measure hydrolysis of trinucleotide at 37 degrees C. Under these catalytic conditions, both nucleotide binding domains hydrolyze ATP, but hydrolysis in A1 is stimulated to a greater degree by Sb(III) than A2. These results suggest that the two homologous halves of the ArsA may be functionally nonequivalent.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, 540 E. Canfield Ave, Detroit, Michigan 48201, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Hiranmoy
authors.initials: H
authors.lastname: Bhattacharjee
authors.forename: Tongqing
authors.initials: T
authors.lastname: Zhou
authors.forename: Barry P
authors.initials: BP
authors.lastname: Rosen
authors.forename: Suresh V
authors.initials: SV
authors.lastname: Ambudkar
authors.forename: Zuben E
authors.initials: ZE
authors.lastname: Sauna
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry; D018053:Arsenites / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D001665:Binding Sites; D002384:Catalysis; D020134:Catalytic Domain; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000502:physiology*; D006868:Hydrolysis; D016623:Ion Pumps / Q000737:chemistry* / Q000502:physiology*; D046911:Macromolecular Substances / Q000737:chemistry; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000502:physiology*; D009711:Nucleotides / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017670:Sodium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013917:Thrombin / Q000737:chemistry; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry
chemical_list: D018053:Arsenites; D001386:Azides; D029968:Escherichia coli Proteins; D016623:Ion Pumps; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D009711:Nucleotides; D017670:Sodium Compounds; C017947:sodium arsenite; C017040:8-azidoadenosine 5'-triphosphate; D014364:Tryptophan; D000255:Adenosine Triphosphate; D013917:Thrombin; C486481:ArsAB ATPase, E Coli