Mitochondrial DNA sequence analysis of two mouse hepatocarcinoma cell lines.
OBJECTIVE
To study genetic difference of mitochondrial DNA (mtDNA) between two hepatocarcinoma cell lines (Hca-F and Hca-P) with diverse metastatic characteristics and the relationship between mtDNA changes in cancer cells and their oncogenic phenotype.


METHODS
Mitochondrial DNA D-loop, tRNA(Met+Glu+Ile) and ND3 gene fragments from the hepatocarcinoma cell lines with 1 100, 1 126 and 534 bp in length respectively were analysed by PCR amplification and restriction fragment length polymorphism techniques. The D-loop 3' end sequence of the hepatocarcinoma cell lines was determined by sequencing.


RESULTS
No amplification fragment length polymorphism and restriction fragment length polymorphism were observed in tRNA(Met+Glu+Ile), ND3 and D-loop of mitochondrial DNA of the hepatocarcinoma cells. Sequence differences between Hca-F and Hca-P were found in mtDNA D-loop.


CONCLUSIONS
Deletion mutations of mitochondrial DNA restriction fragment may not play a significant role in carcinogenesis. Genetic difference of mtDNA D-loop between Hca-F and Hca-P, which may reflect the environmental and genetic influences during tumor progression, could be linked to their tumorigenic phenotypes.
Dai
Ji-Gang
JG
Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. daijigang@vip.sina.com
Lei
Xia
X
Min
Jia-Xin
JX
Zhang
Guo-Qiang
GQ
Wei
Hong
H
D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D051379:Mice; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012352:RNA, Transfer, Glu / Q000235:genetics; D012355:RNA, Transfer, Ile / Q000235:genetics; D012358:RNA, Transfer, Met / Q000235:genetics; D015183:Restriction Mapping
D017931:DNA Primers; D004272:DNA, Mitochondrial; D012352:RNA, Transfer, Glu; D012355:RNA, Transfer, Ile; D012358:RNA, Transfer, Met