[Detection of bcr gene rearrangement in chronic myeloid leukemia by fluorescence in situ hybridization].
OBJECTIVE
To detect bcr gene rearrangement in chronic myeloid leukemia (CML).


METHODS
Bcr rearrangement was detected by fluorescence in situ hybridization (FISH), using 765E3, a yeast artificial chromosome (YAC)-derived probe flanking the breakpoint within bcr gene.


RESULTS
Nine patients with CML were examined, bcr gene rearrangement was revealed in 5 cases in chronic phase, 2 in blastic phase, and one after interferon-alpha therapy. The karyotype of 1 case after autologous bone marrow transplantation (ABMT) was chimera with normal and bcr gene rearrangement chromosomes.


CONCLUSIONS
YAC765E3 is a useful probe for detecting bcr gene rearrangement. FISH technique is likely an important tool for monitoring of treatment and revealing minimal residual disease in CML.
Liu
M
M
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Liu
X
X
Niu
C
C
He
K
K
Su
X
X
Cao
Q
Q
Chen
L
L
Chen
S
S
D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002908:Chronic Disease; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics*; D051562:Proto-Oncogene Proteins c-bcr / Q000235:genetics*
C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr