title: Luminal adenosine stimulates chloride secretion through A1 receptor in mouse jejunum.
abstract: Adenosine is known to stimulate chloride secretion by mouse jejunum. Whereas the receptor on the basolateral side is believed to be A2B, the receptor involved in the luminal effect of adenosine has not been identified. We found that jejuna expressed mRNA for all adenosine receptor subtypes. In this study, we investigated the stimulation of chloride secretion by adenosine in jejuna derived from mice lacking the adenosine receptors of A1 (A1R) and A2A (A(2A)R) or control littermates. The jejunal epithelium was mounted in a Ussing chamber, and a new method on the basis of impedance analysis was used to calculate the short-circuit current (I(sc)) values. Chloride secretion was assessed by the I(sc) after inhibition of the sodium-glucose cotransporter by adding phloridzin to the apical bathing solution. The effect of apical adenosine on chloride secretion was lost in jejuna from mice lacking the A1R. There was no difference in the response to basolaterally applied adenosine or to apical forskolin. Furthermore, in jejuna from control mice, the effect of apical adenosine was also abolished in the presence of 8-cyclopentyl-1,3-dipropylxanthine, a specific A1R antagonist. Responses to adenosine were identical in jejuna from control and A(2A)R knockout mice. This study demonstrates that A1R (and not A(2A)R) mediates the enhancement of chloride secretion induced by luminal adenosine in mice jejunum.
authors.affiliation: Department of Cell Physiology, Free University of Brussels, Brussels, Germany.
authors.forename: Esam
authors.initials: E
authors.lastname: Ghanem
authors.forename: Cecilia
authors.initials: C
authors.lastname: Lövdahl
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Daré
authors.forename: Catherine
authors.initials: C
authors.lastname: Ledent
authors.forename: Bertil B
authors.initials: BB
authors.lastname: Fredholm
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Boeynaems
authors.forename: Willy
authors.initials: W
authors.lastname: Van Driessche
authors.forename: Renaud
authors.initials: R
authors.lastname: Beauwens
mesh_terms: D000241:Adenosine / Q000502:physiology*; D000818:Animals; D002712:Chlorides / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D007583:Jejunum / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D043682:Receptor, Adenosine A1 / Q000235:genetics / Q000502:physiology*; D043683:Receptors, Adenosine A2 / Q000235:genetics / Q000502:physiology*
chemical_list: D002712:Chlorides; D043682:Receptor, Adenosine A1; D043683:Receptors, Adenosine A2; D000241:Adenosine