title: Side-chain assignments of methyl-containing residues in a uniformly 13C-labeled hemoglobin in the carbonmonoxy form.
abstract: Sequence-specific assignment of the methyl groups in large proteins can be obtained from an MQ-(H)CC(m)H(m)-TOCSY experiment on uniformly (13)C-labeled proteins without deuteration (Yang et al., 2004). Here the procedure is further demonstrated on a uniformly (13)C-labeled alpha-chain or beta-chain of human normal adult hemoglobin (65 kDa) in the carbonmonoxy form. In addition, a strategy is presented for assigning protons of methyl-containing residues of uniformly (13)C-labeled large proteins, on the basis of prior methyl assignments based on MQ-(H)CCH-TOCSY and H(C)C(m)H(m)-TOCSY experiments. Assignment of about 80% of the side-chain resonances of methyl-containing residues of carbonmonoxyhemoglobin has been obtained.
authors.affiliation: Department of Biological Sciences, The National University of Singapore, 14 Science Drive 4, Singapore, 117543.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Janel L
authors.initials: JL
authors.lastname: Giovannelli
authors.forename: Nancy T
authors.initials: NT
authors.lastname: Ho
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
authors.forename: Daiwen
authors.initials: D
authors.lastname: Yang
mesh_terms: D002247:Carbon Isotopes; D002263:Carboxyhemoglobin / Q000737:chemistry*; D006801:Humans; D008745:Methylation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D011522:Protons; D002263:Carboxyhemoglobin