Doxorubicin uptake and release from microgel thin films.
We report investigations on the thermally regulated uptake and release of the chemotherapeutic drug doxorubicin from microgel thin films. A spin coating, layer-by-layer (scLbL) assembly approach was used to prepare thin films composed of thermoresponsive poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-AAc) microgels by alternatively exposing a 3-aminopropyltrimethoxysilane (APTMS) functionalized glass substrate to polyanionic pNIPAm-AAc microgels and polycationic poly(allylamine hydrochloride) (PAH). Using this method, 10, 20, and 30 microgel layer films were constructed with uniform layer buildup, as confirmed by quartz crystal microgravimetry (QCM). The films were subsequently loaded with doxorubicin by cycling the temperature of the film in an aqueous doxorubicin solution between 25 and 50 degrees C. Release characteristics were then examined using UV-vis spectroscopy, which revealed temperature-dependent release properties.
Serpe
Michael J
MJ
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.
Yarmey
Kristen A
KA
Nolan
Christine M
CM
Lyon
L Andrew
LA
D004317:Doxorubicin / Q000737:chemistry* / Q000493:pharmacokinetics*; D005782:Gels / Q000138:chemical synthesis / Q000737:chemistry*; D008567:Membranes, Artificial; D008968:Molecular Conformation; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors
D005782:Gels; D008567:Membranes, Artificial; D004317:Doxorubicin