title: [Detection of bcr gene rearrangement in chronic myeloid leukemia by fluorescence in situ hybridization].
abstract: OBJECTIVE
  To detect bcr gene rearrangement in chronic myeloid leukemia (CML).
  
  
  METHODS
  Bcr rearrangement was detected by fluorescence in situ hybridization (FISH), using 765E3, a yeast artificial chromosome (YAC)-derived probe flanking the breakpoint within bcr gene.
  
  
  RESULTS
  Nine patients with CML were examined, bcr gene rearrangement was revealed in 5 cases in chronic phase, 2 in blastic phase, and one after interferon-alpha therapy. The karyotype of 1 case after autologous bone marrow transplantation (ABMT) was chimera with normal and bcr gene rearrangement chromosomes.
  
  
  CONCLUSIONS
  YAC765E3 is a useful probe for detecting bcr gene rearrangement. FISH technique is likely an important tool for monitoring of treatment and revealing minimal residual disease in CML.
authors.affiliation: Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Niu
authors.forename: K
authors.initials: K
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Su
authors.forename: Q
authors.initials: Q
authors.lastname: Cao
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002908:Chronic Disease; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics*; D051562:Proto-Oncogene Proteins c-bcr / Q000235:genetics*
chemical_list: C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr