Influence of cycloheximide on apoptosis in CHO cells, induced by ethane 1,2-dimethanesulphonate (EDS).
Ethane 1,2-dimethanesulphonate (EDS) causes apoptotic death of Leydig cells. Additionally, EDS causes damage of Chinese Hamster Ovary (CHO) cells but the occurrence of apoptosis is not such plentiful. The present study tested whether the inhibition of protein synthesis by cycloheximide (CHX) would influence apoptosis of CHO cells, induced by EDS. The study compounds induced morphological changes in CHO cell typical for apoptosis. An active form of caspase-9 and an alternation of mitochondrial transmembrane potential were also observed. In our study, a more cumulative effect of the CHX and EDS on apoptosis was observed, when both compounds were simultaneously employed. The obtained results indicated that synthesis of antiapoptotic proteins plays a very important role in the inhibition of apoptosis.
Polak
U
U
Department of Radiobiology and Cell Biology, K. Marcinkowski University of Medical Sciences, Poznań, Poland. ulapo@o2.pl
Jankowska
A
A
Warchoł
J B
JB
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016466:CHO Cells; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008698:Mesylates / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D013451:Sulfonic Acids / Q000494:pharmacology*
D008698:Mesylates; D013451:Sulfonic Acids; D003513:Cycloheximide; D053453:Caspase 9; D020169:Caspases; C002994:ethylene dimethanesulfonate