title: [Effect of combination of herbimycin A, an inhibitor of tyrosine kinase, and chemotherapeutic agents on apoptosis of K562 cells].
abstract: OBJECTIVE
  To explore the anti-apoptotic mechanism and the apoptosis-inducing method in chronic myeloid leukemia (CML) cells.
  
  
  METHODS
  K562 cell line was used to observe the effect of combination of herbimycin A (HMA), a tyrosine kinase inhibitor, and chemotherapeutic agents on the induction of apoptosis.
  
  
  RESULTS
  HMA or chemotherapeutic agents could inhibit the proliferation but not significantly induce apoptosis of K562 cells. However, HMA significantly enhanced apoptosis when combined with chemotherapeutic agents. Addition of sulfhydryl compound to the cultures to conjugate HMA completely abrogated this enhancing effect on K562 cells.
  
  
  CONCLUSIONS
  HMA increases the sensitivity of CML cells to chemotherapeutic agents by inactivating tyrosine kinase activity. It is promising that combination of HMA with conventional chemotherapeutic drugs in the treatment of CML.
authors.affiliation: Institute of Hematology, People's Hospital, Beijing Medical University, Beijing 100044.
authors.forename: H
authors.initials: H
authors.lastname: Ren
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Lu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016227:Benzoquinones / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D047029:Lactams, Macrocyclic / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D017828:Rifabutin / Q000031:analogs & derivatives
chemical_list: D000970:Antineoplastic Agents; D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D047428:Protein Kinase Inhibitors; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases