title: Peripheral polymorphonuclear leukocyte priming contributes to oxidative stress in early pregnancy.
abstract: OBJECTIVE
  The cause of elevated blood leukocyte count in pregnancy is unknown. We hypothesized that priming of peripheral polymorphonuclear leukocytes (PMNL) caused this elevation.
  
  
  METHODS
  Eleven women in the first trimester of pregnancy were included in this prospective study. Peripheral venous blood was drawn twice from each woman, before and after a medical abortion (pregnant and nonpregnant, respectively). Complete blood cell count, plasma alkaline phosphatase (ALP), and rate of superoxide release from separated phobrol 12-myristate-13-acetate (PMA)-stimulated PMNL were determined.
  
  
  RESULTS
  The PMNL count in early pregnancy was significantly higher, with a significant increase in the PMNL rate of superoxide release compared to the nonpregnant state. A linear correlation between the rates of superoxide release and PMNL counts before and during pregnancy was found. ALP levels were significantly elevated in early pregnancy.
  
  
  CONCLUSIONS
  The increased PMNL count is probably a compensatory response to PMNL priming. The increased rate of superoxide release from primed PMNL may contribute to oxidative stress in early pregnancy.
authors.affiliation: Department of Obstetrics and Gynecology, Eliachar Research Laboratory, Western Galilee Hospital, Nahariya, Israel. f1965zxcv@hotmail.com
authors.forename: V
authors.initials: V
authors.lastname: Fait
authors.forename: S
authors.initials: S
authors.lastname: Sela
authors.forename: E
authors.initials: E
authors.lastname: Ophir
authors.forename: H
authors.initials: H
authors.lastname: Kreutzer
authors.forename: O
authors.initials: O
authors.lastname: Shnaider
authors.forename: A
authors.initials: A
authors.lastname: Perri
authors.forename: N
authors.initials: N
authors.lastname: Khatib
authors.forename: G
authors.initials: G
authors.lastname: Dourleshter
authors.forename: R
authors.initials: R
authors.lastname: Tendler
authors.forename: J
authors.initials: J
authors.lastname: Bornstein
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D009504:Neutrophils / Q000502:physiology*; D018384:Oxidative Stress; D011247:Pregnancy / Q000502:physiology*; D011261:Pregnancy Trimester, First; D011446:Prospective Studies; D013481:Superoxides
chemical_list: D013481:Superoxides; D000469:Alkaline Phosphatase