[Coxsackievirus B3-induction of tumor necrosis factor and IL-6 of murine monocyte-macrophages].
This study is to observe the infection of coxsackievirus B3 (Cox B3) to murine monocyte-macrophages (Mo/Mpsi), and its induction of tumor necrosis factor (TNF) and IL-6. Cox B3 was capable of infecting murine macrophages as revealed by immunofluorescence and releasing infectious virus particles. Virus infection with < or = 100 TCID50 did not reduce macrophages viability. Cox B3 stimulated the release of monokines from Mo/Mpsi in vitro and in vivo, markedly increasing the concentration of TNF and IL-6. These were found in the plasma and homogenized heart tissue during the acute phase of murine myocarditis induced by Cox B3, and in the culture supernatant of Mo/Mpsi infected by Cox B3.
Sen
Q
Q
Department of Clinical Laboratories, Changhai Hospital, Shanghai 200433.
Xu
Y
Y
Jiang
A
A
Zhang
T
T
D000818:Animals; D002460:Cell Line; D003384:Coxsackievirus Infections / Q000276:immunology*; D029822:Enterovirus B, Human / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D048069:Tumor Necrosis Factors / Q000276:immunology / Q000378:metabolism*
D015850:Interleukin-6; D048069:Tumor Necrosis Factors