[Analysis of clonality with immunoglobulin heavy chain gene rearrangement fingerprinting map in childhood acute lymphoblastic leukemia].
OBJECTIVE
To investigate the types of the immunoglobulin heavy chain (IgH) gene rearrangement and the co-existing clones in childhood acute lymphoblastic leukemia (ALL).


METHODS
Polymerase chain reaction (PCR) amplification and single-strand conformation polymorphism (SSCP) were used to construct the IgH-PCR-SSCP gene fingerprinting.


RESULTS
Of the 34 gene fingerprinting maps obtained from newly diagnosed ALL, 9 (32.14%) were one allelic rearrangement and 15 (53.75%) were biallelic in mono-clonal rearrangements. Four cases (14.29%) with high white cell count and precursor-B immunophenotype were oligoclonal rearrangements.


CONCLUSIONS
There were multiple IgH bi-allelic rearrangements and co-existing clones in childhood ALL.
Liu
J
J
Department of Pediatrics, PLA General Hospital, Beijing 100853.
Lu
S
S
Zhu
P
P
Xue
H
H
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D005455:Fluorescent Antibody Technique; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016130:Immunophenotyping; D008297:Male; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology
D007143:Immunoglobulin Heavy Chains