title: [Expression of DNA-PK in hepato- and cholangio-neoplasms and its significance].
abstract: OBJECTIVE
  To characterize DNA-PKcs and Ku70 expressions in hepato- and cholangio-neoplastic tissues and the association with the degree of malignancy and invasiveness of the tumors.
  
  
  METHODS
  The expression of DNA-PKcs and Ku70 was examined in 47 cases of hepato- or cholangio-neoplasm by immunohistochemistry.
  
  
  RESULTS
  Ku70 was expressed in all of the neoplastic tissues examined and with a little variation in levels. The highest expression was observed in adenocarcinomas and adenomas. There was no statistically significant association between Ku70 expression level and the degree of their malignancy extent or invasiveness. In contrast to Ku 70, a wide variation in expression levels of DNA-Pkcs was observed among different types of neoplastic tissues. The highest ratio of positive expressing cells was detected in hepatocellular carcinomas (92.1%), which was significantly higher than that in cholangioadeno carcinomas (65.3%) and biliary cystadenocarcinomas (51.9%). Low or no expression level was detected in papillary adenoma cases. DNA-PKcs expression of invasive adenomas and adeno-carcinomas (61.2%) was significantly higher than that of non-invasive adenomas and adeno-carcinomas (30.4%). There was no expression observed in the normal tissues adjacent to the tumors.
  
  
  CONCLUSIONS
  DNA-PKcs is expressed in hepato- and cholangio-neoplasms and its variable level of expression is associated with the types of the tumor and their degree of malignancy and invasiveness. DNA-PKcs could be recognized as a new biomarker for liver neoplasm.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Zi-jian
authors.initials: ZJ
authors.lastname: Yu
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Sui
authors.forename: Ying-qin
authors.initials: YQ
authors.lastname: Ding
authors.forename: Zhen-shan
authors.initials: ZS
authors.lastname: Cao
authors.forename: Ping-kun
authors.initials: PK
authors.lastname: Zhou
authors.forename: De-chang
authors.initials: DC
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear / Q000096:biosynthesis / Q000235:genetics; D001650:Bile Duct Neoplasms / Q000201:enzymology*; D001653:Bile Ducts, Intrahepatic / Q000201:enzymology; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D051747:DNA-Activated Protein Kinase / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D000072200:Ku Autoantigen; D008113:Liver Neoplasms / Q000201:enzymology*; D008297:Male; D008875:Middle Aged
chemical_list: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D051747:DNA-Activated Protein Kinase; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen