Influence of cannabinoids on immunoreactivity of regulatory peptides, produced in rat thyroid C cells; preliminary investigations.
Mammalian tissues contain two types of cannabinoid receptors CB1 and CB2. The aim of our study was an evaluation of the influence of a single i.p. injection of a stable analogue of an endogenous cannabinoid anandamide--R-(+)-methanandamide (2.5 mg/kg) and CP 55,940 (0.25 mg/kg), which is an exogenous agonist of CB1 receptors, on the immunoreactivity of regulatory peptides, produced in rat thyroid C cells: calcitonin, CGRP, somatostatin and synaptophysin. This study indicates that a single injection of cannabinoids: R-(+)-methanandamide and CP 55,940 alters the immunoreactivity of regulatory peptides in thyroid parafollicular cells.
Sawicki
B
B
Department of Histology and Embryology, Medical University of Białystok, Poland. sawboghe@amb.edu.pl
Zbucki
R L
RL
Hryniewicz
A
A
Kosiorek
P
P
Bialuk
I
I
Winnicka
M M
MM
Dadan
J
J
D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002186:Cannabinoids / Q000494:pharmacology*; D003511:Cyclohexanols / Q000494:pharmacology; D063388:Endocannabinoids; D008297:Male; D053284:Polyunsaturated Alkamides; D051381:Rats; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000187:drug effects / Q000502:physiology; D043885:Receptor, Cannabinoid, CB2 / Q000187:drug effects / Q000502:physiology; D013004:Somatostatin / Q000378:metabolism; D016708:Synaptophysin / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects / Q000502:physiology*
D001095:Arachidonic Acids; D002186:Cannabinoids; C469526:Cnr2 protein, rat; D003511:Cyclohexanols; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D016708:Synaptophysin; C088155:methanandamide; D013004:Somatostatin; C054649:3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol; D015740:Calcitonin Gene-Related Peptide; C078814:anandamide