[Relationship of antiphospholipoprotein antibodies of venous thrombi with anticoagulation and fibrinolysis].
To study the etiological factors and pathogenisis of venous thrombi and their relations with anticoagulation and fibrinolysis, In 47 patients with venous thrombi anticardiolipin antibody (ACA) was detected by ELISA. lupus anticoagulant (LA) and anti-activative protein C resistance (APCR) were examined by coagulation test; factor V Leiden was determined by PCR; activity of anticoagulation and fibrinolysis of antithrombin (AT), protein C (PC), plasminogen (Plg) were detected by chromophore substrate methods. The results showed that ACA and/or LA were positive in 34% of patients with VT, most of which consisted of ACA IgG and LA; Plg was negative in 9.5% of patients; tPAI elevated in 8.3% of patients (much more than control group, P < 0.005); ATIII, PC, tPA were negative in 4.5%, 4.5%, 2.8% of patients, respectively (no significant difference with control groups, P > 0.05); ATIII, PC and Plg were negative constantly in one patient; factor V Leiden was not detected by PCR. There were no significant differences in anticoagulation and fibrinolysis between antiphospholipoprotein antibody (APA) negative subjects and APA positive subjects, 4 patients of which were positive in APCR, 3 patients were positive in ACA and/or LA, two out of three patients didn't achieved APCR reversion after mixing their blood plasma with normal blood plasma. It is concluded that antiphospholipoprotein antibody and abnormal fibrinolysis were the common pathological factors in venous thrombi. LA and/or ACA disturbs the anticoagulation aspect to develop into acquired APCR which may be a possible cause leading to thrombophilia.
Chen
Zhao-Hui
ZH
Central Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China. czh8830@yahoo.cn.com
Zheng
Yan-Zhen
YZ
Tang
Jian
J
Li
Ning
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D000991:Antithrombins / Q000378:metabolism; D001777:Blood Coagulation; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005165:Factor V / Q000235:genetics; D005260:Female; D005342:Fibrinolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010958:Plasminogen / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011486:Protein C / Q000378:metabolism; D020246:Venous Thrombosis / Q000097:blood* / Q000235:genetics / Q000378:metabolism
D017152:Antibodies, Antiphospholipid; D000991:Antithrombins; D011486:Protein C; C095381:factor V Leiden; D005165:Factor V; D010958:Plasminogen