Effect of prolonged inactivity on skeletal motor nerve terminals during aestivation in the burrowing frog, Cyclorana alboguttata.
This study examined the effect of prolonged inactivity, associated with aestivation, on neuromuscular transmission in the green-striped burrowing frog, Cyclorana alboguttata. We compared the structure and function of the neuromuscular junctions on the iliofibularis muscle from active C. alboguttata and from C. alboguttata that had been aestivating for 6 months. Despite the prolonged period of immobility, there was no significant difference in the shape of the terminals (primary, secondary or tertiary branches) or the length of primary terminal branches between aestivators and non-aestivators. Furthermore, there was no significant difference in the membrane potentials of muscle fibres or in miniature end plate potential (EPP) frequency and amplitude. However, there was a significant decrease in evoked transmitter release characterised by a 56% decrease in mean EPP amplitude, and a 29% increase in the failure rate of nerve terminal action potentials to evoke transmitter release. The impact of this suite of neuromuscular characteristics on the locomotor performance of emergent frogs is discussed.
Hudson
Nicholas J
NJ
School of Life Sciences, The University of Queensland, QLD, Brisbane 4072, Australia.
Lavidis
Nickolas A
NA
Choy
Peng T
PT
Franklin
Craig E
CE
D000818:Animals; D001001:Anura / Q000502:physiology*; D004956:Estivation / Q000502:physiology*; D005071:Evoked Potentials / Q000502:physiology; D005260:Female; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D009043:Motor Activity / Q000502:physiology*; D009045:Motor Endplate / Q000502:physiology; D009046:Motor Neurons / Q000502:physiology*; D018482:Muscle, Skeletal / Q000294:innervation / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology*; D018709:Statistics, Nonparametric; D013569:Synapses / Q000502:physiology