title: Evaluation of protein products of cell cycle regulating genes in gastric cancer.
abstract: Formalin fixed, paraffin embedded tissue samples of 45 gastric carcinomas, resected curatively, were used for the study. An immunohistochemical analysis employed monoclonal antibodies: p53 (No N1581, DAKO) and p27KIP1 (NCL-p27KIP1, Novocastra). Positive nuclear protein expression was assessed at the 30% level. We found no correlations between the expression of either protein and Lauren's classification, the age of patients and tumour localization. Borderline significance of p=0.07 was noted in the association of p53 expression and histological differentiation. However, a decrease of p27 expression and an overexpression of p53 correlated with the presence of lymph node metastases (p<0.01). Simultaneously, the expression of p27 protein in main mass of tumour correlated with the lack of p53 expression in the main mass and lymph node metastases.
authors.affiliation: Department of General Pathomorphology, Medical University of Białystok, Poland. kguzinska@poczta.onet.pl
authors.forename: K
authors.initials: K
authors.lastname: Guzińska-Ustymowicz
authors.forename: M
authors.initials: M
authors.lastname: Gryko
authors.forename: J
authors.initials: J
authors.lastname: Czyzewska
authors.forename: A
authors.initials: A
authors.lastname: Kemona
mesh_terms: D000911:Antibodies, Monoclonal; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery
chemical_list: D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins