Production of nitric oxide, tumor necrosis factor-alpha and interleukin-6 by RAW264.7 macrophage cells treated with lactic acid bacteria isolated from kimchi.
The present study investigated immunopotentiating activities of lactic acid bacteria isolated from kimchi (KLAB) in vitro. A RAW264.7 macrophage cell line was stimulated with four strains of KLAB and two strains of bifidobacteria, and then the production of nitric oxide (NO) and cytokines--tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6)--was determined. Lactobacillus plantarum, a lactic acid bacteria involved in the latent fermentation of kimchi, was the most effective for the generation of NO, TNF-alpha, and IL-6 in macrophage. All strains generated NO, while for the TNF-alpha and IL-6, only L. plantarum had significant activity. Our results indicate that L. plantarum plays an important role in the immunopotentiating activity of kimchi.
Hur
Haeng Jeon
HJ
Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
Lee
Ki Won
KW
Lee
Hyong Joo
HJ
D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D001937:Brassica / Q000382:microbiology*; D005511:Food Handling; D015850:Interleukin-6 / Q000096:biosynthesis*; D007723:Korea; D007934:Leuconostoc / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide