title: Isolation and structural identification of diarabinosyl 8-O-4-dehydrodiferulate from maize bran insoluble fibre.
abstract: The first saccharide ester of a dehydrodiferulic acid (DFA) other than 5-5-DFA has been isolated from maize bran insoluble fibre after acidic hydrolysis and fractionation by gel chromatography and semi-preparative RP-HPLC. HPLC-MS along with 1D, 2D and 3D NMR spectra provided the requisite structural evidence that it is the di-5-O-l-arabinosyl ester of 8-O-4-DFA. Although a range of DFAs have been well authenticated as components released from the cell walls of grasses, the only structural evidence for a DFA attached to polysaccharides had been from 5-5-DFA. The isolation of the 8-O-4-ester demonstrates that polysaccharides in maize cell walls, and presumably in all grasses, are cross-linked through dehydrodiferulates other than 5-5-dehydrodiferulate.
authors.affiliation: Department of Food Chemistry, Institute of Biochemistry and Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Ella
authors.initials: E
authors.lastname: Allerdings
authors.forename: John
authors.initials: J
authors.lastname: Ralph
authors.forename: Paul F
authors.initials: PF
authors.lastname: Schatz
authors.forename: Diana
authors.initials: D
authors.lastname: Gniechwitz
authors.forename: Hans
authors.initials: H
authors.lastname: Steinhart
authors.forename: Mirko
authors.initials: M
authors.lastname: Bunzel
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry* / Q000302:isolation & purification; D004043:Dietary Fiber / Q000032:analysis; D004187:Disaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D003313:Zea mays / Q000737:chemistry*
chemical_list: D003373:Coumaric Acids; D004043:Dietary Fiber; D004187:Disaccharides; C498109:diarabinosyl 8-O-4-dehydrodiferulate