Notes on the radioimmunoassay of human thyrotrophin.
This paper describes the use of screened transfusion plasma as a source of human thyroid stimulating hormone (h-TSH) free protein for standardization of a conventional "double antibody" h-TSH radioimmunoassay. Serum and plasma immunoreactive h-TSH levels have been shown to be stable for up to four days at 4 degrees C and 25 degrees C. A 37 degrees C incubation procedure has been developed which gives an acceptable standard curve, but with some loss of "between batch" precision.
Wood
P J
PJ
Department of Biochemistry, University of Surrey, Guildford, Surrey.
Smith
J
J
Marks
V
V
D004355:Drug Stability; D006801:Humans; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods*; D012015:Reference Standards; D013696:Temperature; D013972:Thyrotropin / Q000097:blood*; D013997:Time Factors
D007457:Iodine Radioisotopes; D013972:Thyrotropin