title: Genotyping of Toxoplasma gondii strains isolated from dogs with neurological signs.
abstract: From 111 dogs with neurological signs admitted in this research in a 22-month period, brain samples of 34 animals were inoculated in mice in order to isolate Toxoplasma gondii. From these 34 dogs, 9 strains of T. gondii were isolated and the genetic characterization performed by restriction analysis (RFLP) of the SAG-2 gene. RFLP analysis showed that four of them were classified as Type I, and five as Type III. The present report is the first description of genotyping of T. gondii isolated from brain samples of naturally infected dogs, in Brazil.
authors.affiliation: Universidade Paranaense (UNIPAR), Umuarama, Paraná, Brazil. silva.av@uol.com.br
authors.forename: Aristeu Vieira
authors.initials: AV
authors.lastname: da Silva
authors.forename: Sandia Bergamaschi
authors.initials: SB
authors.lastname: Pezerico
authors.forename: Vanessa Yuri
authors.initials: VY
authors.lastname: de Lima
authors.forename: Leandro
authors.initials: L
authors.lastname: d'Arc Moretti
authors.forename: Julia Plombom
authors.initials: JP
authors.lastname: Pinheiro
authors.forename: Erica Maeme
authors.initials: EM
authors.lastname: Tanaka
authors.forename: Márcio Garcia
authors.initials: MG
authors.lastname: Ribeiro
authors.forename: Helio
authors.initials: H
authors.lastname: Langoni
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics; D001681:Biological Assay / Q000662:veterinary; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology* / Q000473:pathology; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005838:Genotype; D051379:Mice; D009422:Nervous System Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology* / Q000473:pathology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C067547:surface antigen P22, Toxoplasma