title: Characterisation of iota-carrageenans oligosaccharides with high-performance liquid chromatography coupled with evaporative light scattering detection.
abstract: Enzymatically digested oligo-iota-carrageenans were separated with liquid chromatography, coupled to evaporative light scattering detection. As expected, compared to oligo-kappa-carrageenans, the additional sulphate group in the neocarrabiose unit of iota-carrageenans significantly modified the separation mechanisms on ion-exchange chromatography, porous graphitic carbon and ion-pair chromatography. The oligomers were then isolated and characterised off-line with electrospray ionisation mass spectrometry in positive-ion mode. The tetrasaccharide, hexasaccharide and octasaccharide that were identified were associated with protonated heptylamine molecules whose number depended on the number of sulphate groups.
authors.affiliation: Institut de Chimie Organique et Analytique (ICOA), UMR CNRS 6005, Université d'Orléans, BP 6759, 45067 Orléans Cedex 2, France.
authors.forename: A
authors.initials: A
authors.lastname: Antonopoulos
authors.forename: P
authors.initials: P
authors.lastname: Favetta
authors.forename: M
authors.initials: M
authors.lastname: Lafosse
authors.forename: W
authors.initials: W
authors.lastname: Helbert
mesh_terms: D000837:Anion Exchange Resins; D002351:Carrageenan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D009844:Oligosaccharides / Q000737:chemistry*; D012542:Scattering, Radiation; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000837:Anion Exchange Resins; D009844:Oligosaccharides; D002351:Carrageenan