title: Apical cell membranes are shed into urine from injured podocytes: a novel phenomenon of podocyte injury.
abstract: Previously it was shown that urine from patients with nephritis contains podocytes and their fragments (podocalyxin [PCX]-positive granular structures [PPGS]), reflecting the degree of podocyte injury. The present study was designed to trace PPGS to their origin. Urine samples and renal biopsy specimens from 53 children with nephrotic syndrome and nephritis were examined immunohistochemically. Immunofluorescence studies of kidney sections using an anti-PCX antibody demonstrated that PPGS originated from the glomerulus and flowed into the tubular lumen. Electron microscopic examination revealed that PPGS originated from microvillous or vesicle-like structures on injured podocytes in the glomerulus. For examining the origin of the PPGS, apical, slit-diaphragmatic, and basal portions of the podocytes were specifically stained, revealing that PPGS are composed primarily of apical podocyte membranes. Several newly developed antibodies that are reactive with various segments of the PCX molecule were used to analyze more detailed membrane structures, and it was found that PPGS contained intact PCX molecules, indicating that cell membrane structures are excreted in urine. The quantification of PCX content and podocyte numbers revealed that urinary sediment PCX (u-sed-PCX) content per urinary podocyte was much higher than PCX content per podocyte from isolated glomeruli of normal controls, suggesting that u-sed-PCX are derived from sources other than just the cell debris of detached podocytes. Analysis of the correlation between u-sed-PCX and renal histology revealed that the presence of PPGS reflects acute glomerular injury. In conclusion, podocyte apical cell membranes are shed into the urine from injured podocytes, indicating a previously unrecognized manifestation of podocyte injury.
authors.affiliation: Department of Pediatrics, Yoshida Hospital, Yoshida-machi, Nishikanbara-gun, 959-0242 Niigata, Japan. mhara@yhp-unet.ocn.ne.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Hara
authors.forename: Toshio
authors.initials: T
authors.lastname: Yanagihara
authors.forename: Itaru
authors.initials: I
authors.lastname: Kihara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Higashi
authors.forename: Kotarou
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Kajita
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D016022:Case-Control Studies; D002462:Cell Membrane / Q000473:pathology / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D018076:DNA, Complementary / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005921:Glomerulonephritis / Q000473:pathology* / Q000652:urine; D006801:Humans; D007677:Kidney Function Tests; D007678:Kidney Glomerulus / Q000473:pathology; D008297:Male; D009404:Nephrotic Syndrome / Q000473:pathology* / Q000652:urine; D011336:Probability; D011379:Prognosis; D011507:Proteinuria / Q000652:urine*; D012016:Reference Values; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D012795:Sialoglycoproteins / Q000378:metabolism*; D018709:Statistics, Nonparametric; D014556:Urine / Q000166:cytology*
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D012795:Sialoglycoproteins; C001643:podocalyxin