Phosphoglycerate mutase has essential arginyl residues.
Phosphoglycerate mutase is inactivated by butanedione in borate buffer. Inactivation by 0.13 mM reagent correlates with the modification of one arginyl residue per subunit, and is prevented by either 2, 3-diphosphoglycerate or 3-phosphoglycerate. With 0.50 mM butanedione, inactivation is accompanied by the modification of three arginyl residues per subunit, two of which are protected by the combined presence of cofactor and substrate.
Borders
C L
CL
Department of Chemistry, College of Wooster, Wooster, Ohio 44691, USA.
Wilson
B A
BA
D019794:2,3-Diphosphoglycerate / Q000494:pharmacology; D001120:Arginine / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis / Q000187:drug effects; D003067:Coenzymes / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology; D005988:Glyceric Acids / Q000494:pharmacology; D010736:Phosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*
D003067:Coenzymes; D004852:Epoxy Compounds; D005988:Glyceric Acids; D019794:2,3-Diphosphoglycerate; C007366:diepoxybutane; C005156:3-phosphoglycerate; D001120:Arginine; D010736:Phosphoglycerate Mutase