[Detection of Y chromosome in human and Microtus mandarinus with FISH].
The purpose of the investigation was to detect the Y chromosome of human and Microtus mandarinus with the satellite DNA probe in human's Yq by fluorescence in situ hybridization(FISH). We hybridizied the probe with the metaphase chromosome and interphase nucleus specimens of humanbeing and Microtus mandarinus respectively. The results showed that there was hybridization signal on metaphase chromosome and interphase nucleus of human, but no signal on metaphase chromosome and interphase nucleus of Microtus mandarinus. According to the results,we discussed the homologue between pY3.4 in Y chromosome of humanbeing and DNA of Microtus mandarinus and FISH result of Microtus mandarinus.
Zhu
Bi-Cai
BC
Department of Biology, Xuzhou Normal University, Xuzhou 221116, China. bicaizhu0054@sina.com
Gao
Jian-Guo
JG
Zhang
Zi-Feng
ZF
Zhang
Yong
Y
Gao
Jun-Fang
JF
Hou
Jin-Hui
JH