Role of meltrin {alpha} (ADAM12) in obesity induced by high- fat diet.
Meltrin alpha is a member of the metalloprotease-disintegrin (ADAM) family. In this paper we demonstrate that meltrin alpha is involved in the development of white adipose tissue. Compared with wild-type mice, meltrin alpha(-/-) mice displayed moderate resistance to weight gain induced by a high-fat diet, mainly because of an impaired increase in the number of adipocytes. There was no obvious difference in adipocyte size between wild-type and meltrin alpha(-/-) mice, suggesting normal maturation of adipocytes of the latter under a high-fat diet. Embryonic fibroblasts and stromal-vascular cells lacking meltrin alpha exhibited impaired cell proliferation upon adipogenic stimulation, which was accompanied by moderate defects in adipose differentiation. Addition of culture medium conditioned with wild-type cells in an early phase of adipose differentiation did not restore the defects in the meltrin alpha(-/-) cells. These results uncover the involvement of meltrin alpha in the development of obesity and in adipogenic cell proliferation.
Masaki
Megumi
M
Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
Kurisaki
Tomohiro
T
Shirakawa
Kamon
K
Sehara-Fujisawa
Atsuko
A
D051722:ADAM Proteins; D000072199:ADAM12 Protein; D017667:Adipocytes / Q000502:physiology; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D004041:Dietary Fats / Q000008:administration & dosage*; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009124:Muscle Proteins / Q000235:genetics / Q000502:physiology*; D009765:Obesity / Q000209:etiology*; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction
D004041:Dietary Fats; D008565:Membrane Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C096115:Adam12 protein, mouse; D005947:Glucose