Assay of cortisol in serum by competitive protein binding without solvent extraction.
A method for the determination of serum cortisol by competitive protein binding is proposed in which solvent extraction is replaced by heat inactivation. Results obtained by the new technique correlate well with those obtained by solvent extraction. A saving in manipulative time and glassware is effected when the method is applied in a routine assay.
Morris
R
R
Department of Clinical Endocrinology, The Women's Hospital, Showell Green Lane, Sparkhill, Birmingham B11 4HL.
Holder
G
G
D000818:Animals; D001667:Binding, Competitive; D003276:Contraceptives, Oral / Q000008:administration & dosage; D003429:Cross Reactions; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third / Q000097:blood; D011485:Protein Binding; D011817:Rabbits; D011869:Radioligand Assay / Q000379:methods; D012680:Sensitivity and Specificity; D012997:Solvents; D013997:Time Factors; D014316:Tritium
D003276:Contraceptives, Oral; D012997:Solvents; D014316:Tritium; D006854:Hydrocortisone