Sensitive and specific detection of Xanthomonas campestris pv campestris by PCR using species-specific primers based on hrpF gene sequences.
A sensitive and specific assay was developed to detect bacterial black rot of crucifers caused by Xanthomonas campestris pv. campestris (X. c. pv. campestris), in cabbage seed and plant. Primers XCF and XCR from hrpF homologous to nolX, host recognition protein, were used to amplify a 525 bp DNA fragment. PCR technique was applied to detect the pathogen in naturally infected seed and plant of cabbage. The PCR product was only produced from X. c. pv. campestris among 40 isolates of Xanthomonas strains, Escherichia coli (O157:H7), Pectobacterium carotovorum subsp. carotovorum, and other reference bacteria.
Park
Young Jin
YJ
National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea.
Lee
Byoung Moo
BM
Ho-Hahn
Jang
J
Lee
Gil Bok
GB
Park
Dong Suk
DS
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D001937:Brassica / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D016973:Pectobacterium carotovorum / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000382:microbiology; D012680:Sensitivity and Specificity; D013045:Species Specificity; D016959:Xanthomonas campestris / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C106304:HrpF protein, Xanthomonas campestris