[Clonal analysis of blood cells using X-linked HUMARA gene polymorphism].
OBJECTIVE
To explore the method for clonal analysis of blood cells.


METHODS
Thirty female AML patients and 20 normal female subjects were analysed by the analysis of X-linked HUMARA gene polymorphism and methylation pattern.


RESULTS
The heterozygote rate of HUMARA gene in Chinese women was 88% and the gene had a stable methylation pattern. Out of 20 control females, 17 were heterozygotes and among them 15 were polyclonal, 2 (11.8%) showed skewed X-inactivation. All heterozygote AML were clonal hematopoiesis.


CONCLUSIONS
HUMARA gene polymorphism is a good marker for clonality analysis of blood cells.
Li
K
K
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
Wang
L
L
Hao
Y
Y
Chao
H
H
Meng
Q
Q
Feng
B
B
Tang
K
K
Bian
S
S
D000328:Adult; D001773:Blood Cells / Q000166:cytology* / Q000378:metabolism*; D016022:Case-Control Studies; D002999:Clone Cells; D019175:DNA Methylation; D005260:Female; D050172:Genes, X-Linked / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics; D008297:Male; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics*
C410798:AR protein, human; D011944:Receptors, Androgen