[Cord blood plasma selectively stimulates the expansion of hematopoietic pregenitor cells in vitro].
OBJECTIVE
To explore the effect of cord blood (CB) plasma on the expansion of hematopoietic progenitor cells.


METHODS
The effect of CB plasma on the expansion of CB and bone marrow (BM)-derived hematopoietic progenitor cells was studies and compared with those of adult's peripheral blood (PB) plasma and cytokines such as IL-3 and SCF.


RESULTS
(1) CB plasma increased CFU yields of CB-derived progenitor cells by 2.23 +/- 0.52-fold and this expansion was dependent on CB plasma concentrations supplemented in the cultures. Greater expansion of progenitor cells was found when ABO was unmatched between the CB-derived cells and CB plasma. (2) Stimulating effect of CB plasma on BM-derived progenitor cells was also related to ABO type. CFU yields were increased by 1.55 +/- 0.43-fold when ABO was unmatched and not increased when ABO was matched. (3) Expansion of CB-derived progenitor cells by IL-3 and SCF was revealed. Significant increase in CFU yields occurred in the addition of both the cytokine and the CB plasma. (4) No expansion occurred in CB-and BM-derived cell cultures supplemented with PB plasma.


CONCLUSIONS
There is factor(s) selectively stimulating expansion of progenitor cells in CB plasma. It may be beneficial that CB plasma was transfused with CB-derived stem cell transplantation.
Wu
G
G
Xinhua Hospital of Shanghai Second Medical University, Shanghai.
Ying
D
D
Lin
Z
Z
Wang
Y
Y
Zhou
X
X
Huang
M
M
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D005312:Fetal Blood / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007231:Infant, Newborn; D007377:Interleukin-3 / Q000494:pharmacology; D010949:Plasma / Q000378:metabolism*; D019089:Stem Cell Factor / Q000494:pharmacology
D007377:Interleukin-3; D019089:Stem Cell Factor