title: Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteins.
abstract: The efficiency of viruses in cancer therapy is enhanced by proteins that mediate the fusion of infected cells with their neighbors. It was reported that replication-competent adenovirus particles can spread between nuclei within fusion-generated syncytia. To assess this conjecture, we generated fusogenic adenoviruses that express a balanced ratio of the F and H glycoproteins of measles virus. The viruses displayed enhanced cytotoxicity but largely unchanged replication efficiencies compared to a nonfusogenic virus. Most notably, the virus genomes did not spread through fusion-generated multinuclear cells. Hence, adenovirus replication in syncytia remains largely restricted to initially transduced nuclei.
authors.affiliation: Institut für Virologie, Klinikum der Philipps, Universität Marburg, Marburg, Germany.
authors.forename: German P
authors.initials: GP
authors.lastname: Horn
authors.forename: Sompong
authors.initials: S
authors.lastname: Vongpunsawad
authors.forename: Evelyn
authors.initials: E
authors.lastname: Kornmann
authors.forename: Barbara
authors.initials: B
authors.lastname: Fritz
authors.forename: Dirk P
authors.initials: DP
authors.lastname: Dittmer
authors.forename: Roberto
authors.initials: R
authors.lastname: Cattaneo
authors.forename: Matthias
authors.initials: M
authors.lastname: Dobbelstein
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology*; D015726:Giant Cells; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008561:Membrane Fusion; D011995:Recombination, Genetic; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus