title: [Studies on peripheral blood stem cells mobilization by short course and high-dose G-CSF].
abstract: OBJECTIVE
  To explore the effect of short-course, high dose granulocyte-colony stimulating factor (G-CSF) on peripheral blood stem cell (PBSC) mobolization.
  
  
  METHODS
  G-CSF 5microg/kg was injected subcutaneously twice daily for 3 consecutive days. On the first day and the fourth day the peripheral blood and bone marrow were collected for CFU-GM, BFU-E, CFU-mix assays and CD34/CD38 detection. A single apheresis of over 8000ml blood processing was performed with CS-3000 blood cell separator. The mononuclear cells were prepared for hematopoietic colony assays and CD34/CD38 detection before and after freezing.
  
  
  RESULTS
  After adminitration of G-CSF, the white blood cell count increased significantly. The CFU-GM, BFU-E, CFU-mix yields and CD34+ CD38+ cell and CD34+ CD38- cells also increased significantly (P<0.05). The median of mononuclear cells was 1.56 x 10(8)/kg. After one week freezing, the recovery of mononuclear cells, CFU-GM, BFU-E yields and CD34+ CD38+ cells were about 80% (P>0.05) and CFU-mix yields was 60%; The CD34+ CD38- cells were decreased compared with that of pre-freezing (P<0.05). The median of the reinfused mononuclear cells was 1.27 x 10(8)/kg,CFU-GM 6. 7 x 10(4)/kg, BFU-E 1.6 x 10(4)/kg, CFU-mix 0.32 x 10(4)/kg, CD34- CD38+ cells 1.6 x 10(6)/kg and CD34+ CD38- cells 0.23 x 10(6)/kg.
  
  
  CONCLUSIONS
  Short course and high-dose G-CSF can effectively mobilize the peripheral stem cells. The mononuclear cells, hematopoietic colonies and CD34/CD38 cells were satisfied for the peripheral stem cells rescue.
authors.affiliation: Department of Hematology, General Hospital of Air Force, Beijing.
authors.forename: Y
authors.initials: Y
authors.lastname: Ji
authors.forename: S
authors.initials: S
authors.lastname: Ji
authors.forename: X
authors.initials: X
authors.lastname: Pei
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Ju
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Liu
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor; D051997:ADP-ribosyl Cyclase 1