Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping.
We used the cerebellum as a model to study the morphogenetic and cellular processes underlying the formation of elaborate brain structures from a simple neural tube, using an inducible genetic fate mapping approach in mouse. We demonstrate how a 90 degrees rotation between embryonic days 9 and 12 converts the rostral-caudal axis of dorsal rhombomere 1 into the medial-lateral axis of the wing-like bilateral cerebellar primordium. With the appropriate use of promoters, we marked specific medial-lateral domains of the cerebellar primordium and derived a positional fate map of the murine cerebellum. We show that the adult medial cerebellum is produced by expansion, rather than fusion, of the thin medial primordium. Furthermore, ventricular-derived cells maintain their original medial-lateral coordinates into the adult, whereas rhombic lip-derived granule cells undergo lateral to medial posterior transverse migrations during foliation. Thus, we show that progressive changes in the axes of the cerebellum underlie its genesis.
Sgaier
Sema K
SK
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA.
Millet
Sandrine
S
Villanueva
Melissa P
MP
Berenshteyn
Frada
F
Song
Christian
C
Joyner
Alexandra L
AL
D000818:Animals; D019521:Body Patterning / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D002531:Cerebellum / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D002678:Chimera; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005819:Genetic Markers; D018398:Homeodomain Proteins / Q000235:genetics; D019426:Integrases / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009024:Morphogenesis / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D000099266:Engrailed 2 Protein
D005819:Genetic Markers; D018398:Homeodomain Proteins; D019426:Integrases; D009419:Nerve Tissue Proteins; D000099266:Engrailed 2 Protein; C080330:En1 protein, mouse; C045073:Cre recombinase