title: Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting.
abstract: We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P=0.057, r=0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.
authors.affiliation: AIDS Prevention Initiative Nigeria, Jos University Teaching Hospital, Jos, Nigeria. ereimade@yahoo.co.uk
authors.forename: Godwin E
authors.initials: GE
authors.lastname: Imade
authors.forename: Bitrus
authors.initials: B
authors.lastname: Badung
authors.forename: Sunday
authors.initials: S
authors.lastname: Pam
authors.forename: Oche
authors.initials: O
authors.lastname: Agbaji
authors.forename: Daniel
authors.initials: D
authors.lastname: Egah
authors.forename: Atiene S
authors.initials: AS
authors.lastname: Sagay
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Sankalé
authors.forename: Saidi
authors.initials: S
authors.lastname: Kapiga
authors.forename: John
authors.initials: J
authors.lastname: Idoko
authors.forename: Phyllis
authors.initials: P
authors.lastname: Kanki
mesh_terms: D000328:Adult; D001331:Automation; D018791:CD4 Lymphocyte Count / Q000191:economics / Q000379:methods*; D005434:Flow Cytometry / Q000191:economics / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000191:economics / Q000379:methods; D009549:Nigeria; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity