Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1.
The complete genome of a novel papillomavirus was isolated from a cutaneous papillomatous lesion of a North American porcupine (Erethizon dorsatum) using multiply primed rolling-circle amplification (RCA). The nucleotide sequence, genome organization, and phylogenetic position of the Erethizon dorsatum papillomavirus type 1 (EdPV-1) were determined. EdPV-1 is only distantly related to other benign cutaneous papillomavirus sequences and is the first member of the novel Sigma papillomavirus genus.
Rector
Annabel
A
Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium.
Tachezy
Ruth
R
Van Doorslaer
Koenraad
K
MacNamara
Tracey
T
Burk
Robert D
RD
Sundberg
John P
JP
Van Ranst
Marc
M
D000818:Animals; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary*; D016679:Genome, Viral; D009656:North America; D021141:Nucleic Acid Amplification Techniques; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D046328:Porcupines / Q000821:virology*
D004270:DNA, Circular; D004279:DNA, Viral