[Preparation and characterization of monoclonal antibody against ARL-1 protein].
OBJECTIVE
To prepare monoclonal antibodies against aldose reductase-like(ARL-1)protein.


METHODS
Purified ARL-1 protein (ARL-GST) was used to immunize BALB/c mice, and mAbs were prepared by hybridoma technique. The mAbs against ARL-1 were detected by ELISA, Western blot and immunohistochemical staining respectively.


RESULTS
One clone of hybridoma secreting specific mAb against ARL-1 was obtained. The Ig subclass of mAb was of IgG2b and the titre of the antibody in ascites was 1:4.096 x 10(5). Highexpression of ARL-1 protein in liver cancer was detected.


CONCLUSIONS
The specific mAb against ARL-1 lays the foundation for investigation of functions of ARL-1 protein and relationship between ARL-1 protein and liver cancer.
Shan
Jun
J
Genetics Research Center, Medical College of Southeast University , Nanjing 210009, China.
Zhang
Jian-Qiong
JQ
Ma
Da
D
Bao
Hai-Xun
HX
Miao
Feng-Qin
FQ
Xie
Wei
W
D000449:Aldehyde Reductase / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic; D006825:Hybridomas / Q000276:immunology; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000449:Aldehyde Reductase