title: Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1.
abstract: The complete genome of a novel papillomavirus was isolated from a cutaneous papillomatous lesion of a North American porcupine (Erethizon dorsatum) using multiply primed rolling-circle amplification (RCA). The nucleotide sequence, genome organization, and phylogenetic position of the Erethizon dorsatum papillomavirus type 1 (EdPV-1) were determined. EdPV-1 is only distantly related to other benign cutaneous papillomavirus sequences and is the first member of the novel Sigma papillomavirus genus.
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium.
authors.forename: Annabel
authors.initials: A
authors.lastname: Rector
authors.forename: Ruth
authors.initials: R
authors.lastname: Tachezy
authors.forename: Koenraad
authors.initials: K
authors.lastname: Van Doorslaer
authors.forename: Tracey
authors.initials: T
authors.lastname: MacNamara
authors.forename: Robert D
authors.initials: RD
authors.lastname: Burk
authors.forename: John P
authors.initials: JP
authors.lastname: Sundberg
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary*; D016679:Genome, Viral; D009656:North America; D021141:Nucleic Acid Amplification Techniques; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D046328:Porcupines / Q000821:virology*
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral