Regulation of latent sensory hair cell precursors by glia in the zebrafish lateral line.
The lateral line is a placodally derived mechanosensory organ in anamniotes that detects the movement of water. In zebrafish embryos, a migrating primordium deposits seven to nine clusters of sensory hair cells, or neuromasts, at intervals along the trunk. Postembryonically, neuromasts continue to be added. We show that some secondary neuromasts arise from a pool of latent precursors that are deposited by the primordium between primary neuromasts. Interneuromast cells lie adjacent to the lateral line nerve and associated glia. These cells remain quiescent while they are juxtaposed with the glia; however, when they move away from the nerve they increase proliferation and form neuromasts. If glia are manually removed or genetically ablated by mutations in cls/sox10, hypersensitive (hps), or rowgain (rog), neuromasts precociously differentiate. Transplantation of wt glia into mutants rescues the appropriate temporal differentiation of interneuromast cells. Our studies reveal a role for glia in regulating sensory hair cell precursors.
Grant
Kelly A
KA
Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA.
Raible
David W
DW
Piotrowski
Tatjana
T
D000818:Animals; D030801:Animals, Genetically Modified; D002352:Carrier Proteins / Q000235:genetics; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006609:High Mobility Group Proteins / Q000235:genetics; D008465:Mechanoreceptors / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D040542:Mechanotransduction, Cellular / Q000502:physiology; D009154:Mutation / Q000235:genetics; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D017933:Peripheral Nervous System / Q000196:embryology / Q000378:metabolism; D055757:SOXE Transcription Factors; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics
D002352:Carrier Proteins; D006609:High Mobility Group Proteins; D055757:SOXE Transcription Factors; D029961:Zebrafish Proteins; C442229:sox10 protein, zebrafish; D049452:Green Fluorescent Proteins