title: Schistosoma japonicum: localization of calpain in the penetration glands and secretions of cercariae.
abstract: A monoclonal antibody was generated against the large subunit of Schistosoma japonicum calpain to study the localization and possible function of the molecule in vivo. Mice were immunized with recombinant S. japonicum calpain and polyclonal antisera and a monoclonal antibody specific to schistosome calpain was obtained. In immunohistochemistry, a monoclonal antibody against S. japonicum calpain, KG-2E11, bound weakly to calpain expressed at the surface of adult worm tegument, however, it bound strongly to the cercarial secretions ("footprints") of S. japonicum, emitted from the penetration glands. The present study indicates that calpain is multifunctional as it is expressed at various locations in different developmental stages. Calpain-based vaccines could thus possibly induce protective immunity against cercariae and the following early developing stages.
authors.affiliation: Department of Parasitology and Tropical Public Health, The University of Occupational and Environmental Health Japan, Kitakyusyu, Japan. tkuma@med.uoeh-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumagai
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Maruyama
authors.forename: Mariko
authors.initials: M
authors.lastname: Hato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohmae
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Osada
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kanazawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Ohta
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002154:Calpain / Q000032:analysis* / Q000276:immunology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000517:prevention & control; D014614:Vaccines, Synthetic
chemical_list: D000911:Antibodies, Monoclonal; D014614:Vaccines, Synthetic; D002154:Calpain