Plant regeneration from epicotyl explant of Jatropha curcas.
In vitro epicotyl explants from Jatropha curcas were cultured on MS medium with indole-3-butyric acid (IBA) and 6-benzyladenine (BA). Adventitious buds were directly induced from the surface of epicotyl explants under the condition with the combinations of IBA 0.1 mg/L and BA 0.2-0.7 mg/L. Of which IBA 0.1 mg/L and BA 0.5 mg/L induced the highest regeneration frequency. Shoot regeneration from callus required the combinations of IBA 0.5 mg/L with BA 0.1 mg/L, IBA 0.5 mg/L with BA 0.2 mg/L and IBA 1.0 mg/L with BA 0.5 mg/L, and the most suitable combination was IBA 1.0 mg/L and BA 0.5 mg/L. The health adventitious buds and regenerated shoots could be rooted on growth regulator-free MS medium. Regenerated plants with well developed shoots and roots were successfully transferred to greenhouse, without visible detectable variation.
Wei
Qin
Q
Sichuan Key Lab of Resource Biology and Pharmaceutical Engineering, College of Life Sciences, Sichuan University, Chengdu 610064, China.
Lu
Wei-Da
WD
Liao
Yi
Y
Pan
Shu-Lin
SL
Xu
Ying
Y
Tang
Lin
L
Chen
Fang
F
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D007211:Indoles / Q000494:pharmacology; D031286:Jatropha / Q000502:physiology*; D007701:Kinetin; D011687:Purines; D012038:Regeneration
D001593:Benzyl Compounds; D007211:Indoles; D011687:Purines; C014612:indolebutyric acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin