[Effect of factors on callus biomass and synthetic mass of hypericin in Hypericum perforatum].
OBJECTIVE
To study the effect of several factors on the quantity of hypericin in H. perforatum callus.


METHODS
High efficiency liquid phase chromatography and plant tissue culture were applied.


CONCLUSIONS
When the ratio of nitro-nitrogen to amina-nitrogen is 3:1, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.
Xu
Ming-shu
MS
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Huang
Lu-qi
LQ
Chen
Mei-lan
ML
Ye
He-chun
HC
Li
Guo-feng
GF
D000873:Anthracenes; D003470:Culture Media; D020902:Hypericum / Q000254:growth & development* / Q000378:metabolism; D008358:Mannose / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology*; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D011205:Povidone / Q000031:analogs & derivatives / Q000494:pharmacology; D046509:Tissue Culture Techniques
D000873:Anthracenes; D003470:Culture Media; C077842:polyvinylpolypyrrolidone; D010569:Perylene; C004965:hypericin; D011205:Povidone; D009584:Nitrogen; D008358:Mannose