Intranasal immunisation using recombinant Lactobacillus johnsonii as a new strategy to prevent allergic disease.
We have previously demonstrated the induction of a specific anti-IgE response in vivo by parenteral immunisation of rhesus monkeys using short IgE mimotopes or an anti-idiotypic antibody mimicking an IgE epitope. Such specific anti-IgE responses may be of clinical benefit for atopic patients. In this study, we examined the potential for a more convenient therapy via mucosal immunisation using live recombinant Lactobacillus johnsonii (Lj) as a vaccine delivery vehicle. Either an anti-idiotypic scFv or an IgE mimotope were expressed on the surface of Lj as fusion proteins using the cell wall anchored proteinase PrtB from Lactobacillus delbrueckii subsp. bulgaricus. The recombinant Lj were shown to express the heterologous fusion proteins and were specifically recognised by the corresponding anti-human IgE monoclonal antibody. Subcutaneous and intranasal immunisation of mice with recombinant Lj, expressing these fusion proteins induced a systemic IgG response against human IgE. Our data suggest that recombinant Lactobacilli expressing IgE epitopes may represent a novel means of vaccination to induce a beneficial anti-IgE response.
Scheppler
Lorenz
L
Institute of Immunology, Inselspital, 3010 Bern, Switzerland.
Vogel
Monique
M
Marti
Pamela
P
Müller
Lorenz
L
Miescher
Sylvia M
SM
Stadler
Beda M
BM
D000595:Amino Acid Sequence; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D005260:Female; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D007074:Immunoglobulin G / Q000276:immunology; D007778:Lactobacillus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D011994:Recombinant Proteins