title: Inhibition of Bcl10-mediated activation of NF-kappa B by BinCARD, a Bcl10-interacting CARD protein.
abstract: We have identified a novel CARD-containing protein from EST database. BinCARD (Bcl10-interacting protein with CARD). BinCARD was ubiquitously expressed. Co-immunoprecipitation, In vitro binding, mammalian two-hybrid, and immunostaining assays revealed that BinCARD interacted with Bcl10 through CARD. BinCARD potently suppressed NF-kappa B activation induced by Bcl10 and decreased the amounts of phosphorylated Bcl10. Mutations at the residue Leu17 or Leu65, which is highly conserved in CARD, abolished the inhibitory effects of BinCARD on both Bcl10-induced activation of NF-kappa B and phosphorylation of Bcl10. Further, expression of BinCARD inhibited Bcl10 phosphorylation induced by T cell activation signal. These results suggest that BinCARD interacts with Bcl10 to inhibit Bcl10-mediated activation of NF-kappa B and to suppress Bcl10 phosphorylation.
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Woo
authors.forename: Gil-Sun
authors.initials: GS
authors.lastname: Hong
authors.forename: Joon-Il
authors.initials: JI
authors.lastname: Jun
authors.forename: Dong-Hyung
authors.initials: DH
authors.lastname: Cho
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Choi
authors.forename: Ho-June
authors.initials: HJ
authors.lastname: Lee
authors.forename: Chul-Woong
authors.initials: CW
authors.lastname: Chung
authors.forename: In-Ki
authors.initials: IK
authors.lastname: Kim
authors.forename: Dong-Gyu
authors.initials: DG
authors.lastname: Jo
authors.forename: Jong-Ok
authors.initials: JO
authors.lastname: Pyo
authors.forename: John
authors.initials: J
authors.lastname: Bertin
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Jung
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000074018:B-Cell CLL-Lymphoma 10 Protein; D015153:Blotting, Western; D053468:CARD Signaling Adaptor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D017124:Conserved Sequence; D004252:DNA Mutational Analysis; D015536:Down-Regulation; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D019169:Jurkat Cells; D007930:Leucine / Q000737:chemistry / Q000235:genetics; D008156:Luciferases / Q000378:metabolism; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D053468:CARD Signaling Adaptor Proteins; C585892:CARD19 protein, human; D002352:Carrier Proteins; D016328:NF-kappa B; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D008156:Luciferases; D005982:Glutathione Transferase; D007930:Leucine