Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods.
The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. The results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing.
Berzaghi
Rodrigo
R
Department of Microbiology, Immunology and Parasitology, Federal Univeristy of São Paulo, São Paulo, SP., Brazil.
da Silva
Silvia Helena Marques
SH
de Camargo
Zoilo Pires
ZP
D000946:Antigens, Fungal; D018929:Cell Culture Techniques; D002999:Clone Cells; D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008846:Micromanipulation; D010228:Paracoccidioides / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*
C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D003470:Culture Media; D005656:Fungal Proteins; D006023:Glycoproteins