Glucose sensor based on glucose oxidase immobilized by zirconium phosphate.
Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. Reportedly, alpha-ZrP is one of the candidates that are expected to improve the stability of enzymes immobilized on solid surfaces. We intercalated GOxs into ZrP (GOx/ZrP), cast the GOx/ZrP suspension in polyvinylalcohol on a platinum electrode, and dried it in a vacuum oven. The morphological layered structure was investigated by scanning electron microscopy. The enzymatic activities, which were determined by open-circuit potentiometric technique, reached the highest when GOxs were immobilized in ZrP at ca. pH 5. In vitro tests showed good linear responses in the 0-25 mM range and the sensitivity of 0.14 nA mM(-1) at 0.4 V vs. Ag/AgCl. The sensors, as made, were stable for more than 3 days within a limited deterioration.
Park
Sejin
S
Department of Chemistry, Sungshin Women's University, 249-1, Dongsun-dong, Seongbuk-gu, Seoul 136-742, Korea.
Chung
Taek Dong
TD
Kang
Sun Kil
SK
Jeong
Ran-A
RA
Boo
Hankil
H
Kim
Hee Chan
HC
D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D010984:Platinum / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012680:Sensitivity and Specificity; D015040:Zirconium / Q000737:chemistry*
D004800:Enzymes, Immobilized; C027006:zirconium phosphate; D010984:Platinum; D011142:Polyvinyl Alcohol; D015040:Zirconium; D005949:Glucose Oxidase; D005947:Glucose