Vaccination with an inactivated virulent feline immunodeficiency virus engineered to express high levels of Env.
An inactivated virus vaccine was prepared from a pathogenic isolate of feline immunodeficiency virus containing a mutation that eliminated an endocytic sorting signal in the envelope glycoprotein, increasing its expression on virions. Cats immunized with inactivated preparations of this modified virus exhibited strong titers of antibody to Env by enzyme-linked immunosorbent assay. Evidence of protection following challenge demonstrated the potential of this approach to lentiviral vaccination.
Hosie
Margaret J
MJ
Retrovirus Research Laboratory, Institute of Comparative Medicine, University of Glasgow, Bearsden Rd., Glasgow G61 1QH, United Kingdom. m.hosie@vet.gla.ac.uk
Klein
Dieter
D
Binley
James M
JM
Dunsford
Thomas H
TH
Jarrett
Oswald
O
Neil
James C
JC
Knapp
Elzbieta
E
Giannecchini
Simone
S
Matteucci
Donatella
D
Bendinelli
Mauro
M
Hoxie
James A
JA
Willett
Brian J
BJ
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002371:Cat Diseases / Q000517:prevention & control / Q000821:virology; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000517:prevention & control* / Q000821:virology; D015686:Gene Products, env / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D014611:Vaccination / Q000662:veterinary*; D015164:Vaccines, Inactivated / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D015686:Gene Products, env; D015164:Vaccines, Inactivated; D014765:Viral Vaccines