Effect of chronic administration of ethanol on GABAA receptor assemblies derived from alpha2-, alpha3-, beta2- and gamma2-subunits in the rat cerebral cortex.
Chronic administration of ethanol decreased the immunoprecipitation of the [(3)H]flunitrazepam binding activity for GABA(A) receptor assemblies derived from alpha(2)-, alpha(3)- and gamma(2)-subunits in the rat cerebral cortex. However, the [(3)H]muscimol binding sites derived from these subunits were not affected. Thus, chronic ethanol causes the down-regulation of the benzodiazepine sites derived from the alpha(2)-, alpha(3)- and gamma(2)-subunits without affecting the GABA binding sites.
Mehta
Ashok K
AK
Department of Pharmacology, M.C. 7764, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
Ticku
Maharaj K
MK
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism / Q000494:pharmacology; D018755:GABA Agonists / Q000378:metabolism / Q000494:pharmacology; D018757:GABA Modulators / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D009118:Muscimol / Q000378:metabolism / Q000494:pharmacology; D021122:Protein Subunits; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000737:chemistry / Q000378:metabolism*; D014316:Tritium
D002492:Central Nervous System Depressants; D018755:GABA Agonists; D018757:GABA Modulators; D021122:Protein Subunits; D011963:Receptors, GABA-A; D014316:Tritium; D009118:Muscimol; D000431:Ethanol; D005445:Flunitrazepam