Representative appressorium stage cDNA library of Magnaporthe grisea.
A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.
Lu
Jian-ping
JP
School of Life Sciences, Zhejiang University, Hangzhou 310029, China.
Liu
Tong-bao
TB
Yu
Xiao-yun
XY
Lin
Fu-cheng
FC
D003001:Cloning, Molecular / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D020082:Magnaporthe / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D004271:DNA, Fungal