[Effect of danshensu, protocatechualdehyde and danshen injection on calcium ion concentration in cytoplasm of human erythrocytes].
OBJECTIVE
To study the predominant calcium-antagonist components of Danshen injection.


METHODS
The effects of danshensu, protocatechualdehyde and Danshen injection on calcium concentration in cytoplasm of erythrocytes were examined in vitro by the fluorescent Ca+ -chelator fura-2.


RESULTS
Either DS182 or PCAD can decrease in dose-dependent cytosolic free calcium concentration in human erythrocytes. They had additive effect when mixed, which was similar to Danshen injection.


CONCLUSIONS
DS182 and PCAD may be predominant calcium-antagonist components of Danshen injection.
Shen
Ling-hong
LH
Department of Cadiology, Renji Hospital of Shanghai Second Medical University, Shanghai 200001, China. shenlinghong@medmail.com.cn
Wang
Bin-yao
BY
Wang
Chang-qian
CQ
Xie
Xiu-lan
XL
Yu
Guo-rui
GR
Yao
Zhong-yi
ZY
Zhu
Yuan-long
YL
Yang
Bao-jin
BJ
D000328:Adult; D001547:Benzaldehydes / Q000302:isolation & purification / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002396:Catechols / Q000302:isolation & purification / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007267:Injections; D007773:Lactates / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D010946:Plants, Medicinal / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry
D001547:Benzaldehydes; D002396:Catechols; D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde; D002118:Calcium