Desensitization and resensitization are independently regulated in human recombinant GluR subunit coassemblies.
AMPA-type glutamate receptor (GluR) channels are the most abundant excitatory transmitter receptors of the central nervous system. Four subunits with different posttranscriptional modifications and flip/flop splice variants are known. In vivo they occur as tetrameric heteromeric receptors. In the present study we analyzed the time course of desensitization (tau(D)) and resensitization (tau(rec)) kinetics of different homomeric (coassembly of splice or editing variants of one subunit) and heteromeric (coassembly of different subunits) GluR channels. We found that tau(D) had intermediate values depending on the amount of cDNA of the respective subunit at all heteromeric and homomeric GluR channels tested. The same holds true for tau(rec) except GluR2 flip channels were coexpressed with GluR1 channels. In this case, tau(rec) had values close to that of fast resensitizing GluR2 flip channels, even in the case of an abundance of GluR1 cDNA.
Schlesinger
Friedrich
F
Neurological Department of Medical School Hannover, 30623 Hannover, Germany.
Tammena
Derk
D
Krampfl
Klaus
K
Bufler
Johannes
J
D017398:Alternative Splicing / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002490:Central Nervous System / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008564:Membrane Potentials / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics*
D018076:DNA, Complementary; D020033:Protein Isoforms; D021122:Protein Subunits; D018091:Receptors, AMPA; D011993:Recombinant Fusion Proteins; C104722:glutamate receptor ionotropic, AMPA 2