He-Ne laser effects on blood microcirculation during wound healing: a method of in vivo study through laser Doppler flowmetry.
OBJECTIVE
Low-intensity laser therapy (LILT) is widely used for wound healing promotion and its mechanism of action may be due to an enhancement of blood supply. The aim of this study was to evaluate blood flow alterations in a wound healing model, using laser Doppler flowmetry (LDF) associated with a normalized perfusion parameter.


METHODS
An injury was provoked in 15 rats and blood flow was measured periodically over a period of 21 days. Control groups were established to evaluate LDF and He-Ne laser effects on microcirculation. A 1 J/cm(2) dose was utilized, with 6 mW/cm(2) irradiance.


RESULTS
The results demonstrated flow alterations provoked by lesion, and inflammatory response (P < 0.05). There were no statistical differences between groups.


CONCLUSIONS
The results did not show a significant sustained effect on microcirculation with this He-Ne dose.
Núñez
Silvia Cristina
SC
Centro de Lasers e aplicacoes-Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, Brazil 05508-000. silnunez@aol.com
Nogueira
Gessé E C
GE
Ribeiro
Martha S
MS
Garcez
Aguinaldo S
AS
Lage-Marques
José L
JL
D000818:Animals; D017078:Laser-Doppler Flowmetry; D028022:Low-Level Light Therapy / Q000379:methods*; D008833:Microcirculation / Q000000981:diagnostic imaging / Q000528:radiation effects*; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D014463:Ultrasonography; D014945:Wound Healing / Q000528:radiation effects*