[Study on the production condition of extraction in combination hydrolysis in situ for isolating diosgenin].
OBJECTIVE
To study the production technology of extraction in combination hydrolysis in situ for isolating diosgenin from Dioscorea nipponica by orthogonal design.


METHODS
The optimum production conditions were investigated with the recovery of diosgenin as an index by extraction in combination hydrolysis in situ, and were compared with the traditional method.


RESULTS
Extraction in combination hydrolysis in situ conducted in 1.5 mol x L(-1) sulfuric acid of water containing 75% isopropanol at 100 degrees C for 4.5 h could get higher recovery of diosgenin than traditional methods.


CONCLUSIONS
This production technology can get higher recovery of diosgenin, and it is simple, time and money saving.
Wang
Jun
J
School of Biological and Environmental Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China. wangjunjs@tom.com
Chen
Jun
J
Yang
Ke-di
KD
Yang
Liu-qing
LQ
Ouyang
Zhen
Z
D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000302:isolation & purification*; D006868:Hydrolysis; D011312:Pressure; D013464:Sulfuric Acids; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D013464:Sulfuric Acids; D004144:Diosgenin; C033158:sulfuric acid