Medicinal chemistry, metabolic profiling and drug target discovery: a role for metabolic profiling in reverse pharmacology and chemical genetics.
Comprehensive analysis of the metabolome can contribute to mechanism of action studies for small molecules discovered in phenotypic screens. Examples are presented in this overview of the rapidly developing field of "metabolic profiling." These examples include the use of NMR in gene function analysis, GC-based studies on the identification of metabolic pathways affected by PPAR-gamma agonists, applications of Fourier-transform MS and the use of stable isotope-based metabolic profiling (SIDMAP) to investigate metabolic adaptive changes induced by effective anticancer agents.
Harrigan
George G
GG
Global High Throughput Screening, Pfizer Corporation, Chesterfield, MO 63198, USA. george.g.harrigan@pfizer.com
Brackett
Daniel J
DJ
Boros
László G
LG
D000818:Animals; D002626:Chemistry, Pharmaceutical; D019295:Computational Biology; D016172:DNA Fingerprinting; D016503:Drug Delivery Systems; D006801:Humans; D008660:Metabolism / Q000235:genetics*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010597:Pharmacogenetics
D004364:Pharmaceutical Preparations