[Partial nucleotide sequencing of hepatitis E virus isolated from sera of patients with acute sporadic hepatitis E in China].
28 hepatitis E virus (HEV) cDNA isolates were obtained from 41 sera of patients with acute sporadic hepatitis E (HE) in Shenzhen, Changchun and Hangzhou by a reverse transcription nested polymerase chain reaction (RT-nPCR) and 3 of the 28 RT-nPCR products of HEV cDNA ORF2 region were sequenced. The nucleotide homology of these three HEV isolates was 80.2%, 79.9% and 79.4%, respectively, as compared with the Maxico strain of HEV (M); 93.4%, 92.3% and 93.8% with the Burma epidemic strain of HEV(B) (Ep); 95.5%, 93.9% and 95.1% with the Burma sporadic strain of HEV(B) (Sp); 97.0%, 96.5% and 95.9% with the Xinjiang epidemic strain of HEV CH 1.1. It is demonstrated that these three isolates of HEV obtained from sera of acute sporadiic hepatitis E cases have a higher degree of homology with the Xinjiang strain of HEV CH 1.1 and the Burma strain of HEV(B). The data indicate that these three HEV isolates may belong to HEV(B) subtype, as the HEV CH 1.1 does.
Zhu
X
X
Department of Microbiology, Beijing Medical University 100083.
Zhuang
H
H
Zhu
W
W
Zhu
Y
Y
Ma
W
W
Chen
Y
Y
Lou
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D016751:Hepatitis E / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D012367:RNA, Viral