The C-terminal chromodomain-like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY.
MAGGY is a Ty3/Gypsy retrotransposon, which was identified in the rice blast fungus Magnaporthe oryzae. Some Ty3/Gypsy retrotransposons, including MAGGY, contain a chromodomain-like module (CLM) in the C-terminus of the integrase domain. We have made a series of MAGGY mutants to examine the role of the CLM in the transposition activity of the element. Introduction of a mutation at different positions in the MAGGY integrase revealed that a loss or alteration of the CLM resulted in a drastic decrease in the transposition activity of the element. Our results indicate that the CLM may confer high transposition activity to the element.
Nakayashiki
Hitoshi
H
Laboratory of Plant Pathology, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan. hnakaya@kobe-u.ac.jp
Awa
Tomoko
T
Tosa
Yukio
Y
Mayama
Shigeyuki
S
D000595:Amino Acid Sequence; D004271:DNA, Fungal / Q000235:genetics; D019426:Integrases / Q000737:chemistry* / Q000235:genetics; D007438:Introns / Q000235:genetics; D020082:Magnaporthe / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D018626:Retroelements; D020079:Terminal Repeat Sequences / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics
D004271:DNA, Fungal; D018626:Retroelements; D019426:Integrases