title: Differentiation, maturation, and survival of dendritic cells by osteopontin regulation.
abstract: Dendritic cells (DCs) are antigen-presenting cells with the ability to induce primary immune responses necessary in innate immunity and adaptive immunity. Osteopontin (OPN) is a secreted acidic phosphoprotein containing an arginine-glycine-aspartate sequence and has been suggested to play an important role in early cellular immune responses. The interaction between DCs and OPN has not been clarified. We hypothesized that there is an important interaction between DCs and OPN, which is an indispensable extracellular matrix component in early cellular immune responses. Human monocyte-derived DCs synthesized OPN especially during the differentiation from monocytes to immature DCs. By blocking of OPN with anti-OPN antibody, cultured DCs became smaller and expressed lower levels of costimulatory molecules and major histocompatibility complex class II antigens than untreated DCs. Furthermore, DCs treated with anti-OPN antibody easily underwent apoptosis. These results suggest that human DCs can produce OPN and that OPN may play a role in the differentiation, maturation, and survival of DCs by autocrine and/or paracrine pathways.
authors.affiliation: Department of Respiratory Medicine, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
authors.forename: Kodai
authors.initials: K
authors.lastname: Kawamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iyonaga
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ichiyasu
authors.forename: Junji
authors.initials: J
authors.lastname: Nagano
authors.forename: Moritaka
authors.initials: M
authors.lastname: Suga
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin