title: [Protein splicing and its application in protein engineering].
abstract: Protein splicing, which is an intein mediated posttranslational processing, involves a series of intramolecular cleavage-ligation reactions. Intein is an intervening polypeptide which can catalytic self-cleavage from a pre-protein accompanied by the concomitant joining of the two flanking polypeptides (the extein) through a peptide bond. Protein splicing not only enriches genetic theory of posttranslational processing, but also have wide application prospect.
authors.affiliation: College of Biology, Zhejiang University, Hangzhou 310027, China. dmh11617@sohu.com
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Hao
authors.forename: Kai-Xian
authors.initials: KX
authors.lastname: Qian
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Shen
mesh_terms: D047669:Exteins / Q000235:genetics; D047668:Inteins / Q000235:genetics; D015202:Protein Engineering; D019154:Protein Splicing / Q000235:genetics; D012326:RNA Splicing