title: Aetiological agents of vaginitis in Nigerian women.
abstract: This study focuses on the identification of aetiological agents of vaginitis in Nigerian women. Study subjects are drawn from patients presenting with lower abdominal pain, vaginal discharge and itching at the gynaecology clinic of Lagos University Teaching Hospital and at the Clinical Centre of the Nigerian Institute of Medical Research, Yaba, Lagos, between January 2001 and July 2002. A total of 250 patients gave informed consent to participate in the study. The patients also had pre- and post-test human immunodeficiency virus (HIV) counselling. Each patient completed a questionnaire in order to provide biographical data, past clinical history and socio-economic background information. A cervical swab (CS) and a high-vaginal swab (HVS) were obtained from each patient. Swab samples were examined for pH and under light microscopy by Gram's stain and as wet preparations in 10% potassium hydroxide. Subsequently, samples were cultured on appropriate media at optimal conditions and a drug sensitivity profile for all isolates was determined by standard methods. Blood samples were screened and confirmed for HIV antibodies. Bacterial, fungal and parasitic pathogens were identified or isolated in samples from 241 (96.4%) of the women. Bacterial agents (Neisseria, Streptococcus and Staphylococcus species) were predominant in 128 (51.2%) patients, followed by fungi in 108 (43.2%) and parasites (Trichomonas vaginalis) in five (2.0%). Sensitivity to ciprofloxacin was seen in 40% of Staphylococcus species and in 90% of Neisseria species. Positive HIV serology was seen in 25 (10%) of the 250 women studied, 20 (80%) of which had concurrent microbial infections. Overall, a broad spectrum of microbial agents were shown to be responsible for vaginitis in the group of patients studied.
authors.affiliation: Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, P.M.B 2013, Yaba, Lagos, Nigeria. mnotuonye@yahoo.com
authors.forename: N M
authors.initials: NM
authors.lastname: Otuonye
authors.forename: N N
authors.initials: NN
authors.lastname: Odunukwe
authors.forename: E O
authors.initials: EO
authors.lastname: Idigbe
authors.forename: O D
authors.initials: OD
authors.lastname: Imosemi
authors.forename: S I
authors.initials: SI
authors.lastname: Smith
authors.forename: R C
authors.initials: RC
authors.lastname: Chigbo
authors.forename: M
authors.initials: M
authors.lastname: Bamidele
authors.forename: C T
authors.initials: CT
authors.lastname: Oparaugo
authors.forename: A G
authors.initials: AG
authors.lastname: Mafe
authors.forename: A Z
authors.initials: AZ
authors.lastname: Musa
mesh_terms: D000293:Adolescent; D000328:Adult; D000890:Anti-Infective Agents / Q000627:therapeutic use; D002939:Ciprofloxacin / Q000627:therapeutic use; D005260:Female; D006679:HIV Seropositivity / Q000150:complications; D006801:Humans; D008875:Middle Aged; D009181:Mycoses / Q000150:complications; D016870:Neisseriaceae Infections / Q000150:complications; D013203:Staphylococcal Infections / Q000150:complications; D013290:Streptococcal Infections / Q000150:complications; D014247:Trichomonas Vaginitis / Q000209:etiology; D014627:Vaginitis / Q000209:etiology*
chemical_list: D000890:Anti-Infective Agents; D002939:Ciprofloxacin