title: [Clonal analysis of blood cells using X-linked HUMARA gene polymorphism].
abstract: OBJECTIVE
  To explore the method for clonal analysis of blood cells.
  
  
  METHODS
  Thirty female AML patients and 20 normal female subjects were analysed by the analysis of X-linked HUMARA gene polymorphism and methylation pattern.
  
  
  RESULTS
  The heterozygote rate of HUMARA gene in Chinese women was 88% and the gene had a stable methylation pattern. Out of 20 control females, 17 were heterozygotes and among them 15 were polyclonal, 2 (11.8%) showed skewed X-inactivation. All heterozygote AML were clonal hematopoiesis.
  
  
  CONCLUSIONS
  HUMARA gene polymorphism is a good marker for clonality analysis of blood cells.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Hao
authors.forename: H
authors.initials: H
authors.lastname: Chao
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.forename: B
authors.initials: B
authors.lastname: Feng
authors.forename: K
authors.initials: K
authors.lastname: Tang
authors.forename: S
authors.initials: S
authors.lastname: Bian
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000166:cytology* / Q000378:metabolism*; D016022:Case-Control Studies; D002999:Clone Cells; D019175:DNA Methylation; D005260:Female; D050172:Genes, X-Linked / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics; D008297:Male; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics*
chemical_list: C410798:AR protein, human; D011944:Receptors, Androgen