title: Low sodium dodecyl sulfate concentrations inhibit tobacco mosaic virus coat protein amorphous aggregation and change the protein stability.
abstract: Effects of low SDS concentrations on amorphous aggregation of tobacco mosaic virus (TMV) coat protein (CP) at 52 degrees C and on the protein structure were studied. It was found that SDS completely inhibits the TMV CP (11.5 microM) unordered aggregation at the detergent/CP molar ratio of 15 : 1 (0.005% SDS). As judged by fluorescence spectroscopy, these SDS concentrations did not prevent heating-induced disordering of the large-distance part of the TMV CP subunit, including the so-called "hydrophobic girdle". At somewhat higher SDS/protein ratio (40 : 1) the detergent completely disrupted the TMV CP hydrophobic girdle structure even at room temperature. At the same time, these low SDS concentrations (15 : 1, 40 : 1) strongly stabilized the structure of the small-distance part of the TMV CP molecule (the four alpha-helix bundle) against thermal disordering as judged by the far-UV (200-250 nm) CD spectra. Possible mechanisms of TMV CP heating-induced unordered aggregation initiation are discussed.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
authors.forename: E R
authors.initials: ER
authors.lastname: Rafikova
authors.forename: Yu V
authors.initials: YV
authors.lastname: Panyukov
authors.forename: A M
authors.initials: AM
authors.lastname: Arutyunyan
authors.forename: L S
authors.initials: LS
authors.lastname: Yaguzhinsky
authors.forename: V A
authors.initials: VA
authors.lastname: Drachev
authors.forename: E N
authors.initials: EN
authors.lastname: Dobrov
mesh_terms: D036022:Capsid Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D005453:Fluorescence; D008958:Models, Molecular; D015394:Molecular Structure; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D014027:Tobacco Mosaic Virus / Q000737:chemistry*
chemical_list: D036022:Capsid Proteins; D012967:Sodium Dodecyl Sulfate