Detection of human influenza A viruses by loop-mediated isothermal amplification.
Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test.
Poon
Leo L M
LL
Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR. llmpoon@hkucc.hku.hk
Leung
Cynthia S W
CS
Chan
Kwok H
KH
Lee
Jack H C
JH
Yuen
Kwok Y
KY
Guan
Yi
Y
Peiris
Joseph S M
JS
D000199:Actins / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral