[The expression pattern of L-type voltage-gated Ca2+ channel in rat cochlea].
OBJECTIVE
To investigate the molecular basis of the voltage-gated Ca2+ channel (VGCC) which controls the afferent synaptic neurotransmitters release in inner hair cell and their cellular expression.


METHODS
In this study we used different and complementary approaches to determine which types of 4 subunits of VGCC were expressed in the organ of Corti.


RESULTS
RT-PCR analysis showed that alpha1C and alpha1D were present in the organ of Corti total RNA extract, and the corresponding antisense riboprobes could detect the expression of alpha1C and alpha1D in IHCs and OHCs.


CONCLUSIONS
The voltage-gated Ca2+ channel expressed in sensory cell in organ of Corti are alpha1C and alpha1D subtypes.
Shen
Weidong
W
Department of Otolaryngology-Head and Neck Surgery, PLA General Hospital, Beijing, 100853, China.
Hu
Yinyan
Y
Yang
Weiyan
W
Han
Dongyi
D
D000818:Animals; D020746:Calcium Channels, L-Type / Q000235:genetics* / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism; D005260:Female; D006198:Hair Cells, Auditory / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009435:Synaptic Transmission
D020746:Calcium Channels, L-Type; D012333:RNA, Messenger