Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry.
A crucial aim upon the completion of the human genome is the verification and functional annotation of all predicted genes and their protein products. Here we describe the mapping of peptides derived from accurate interpretations of protein tandem mass spectrometry (MS) data to eukaryotic genomes and the generation of an expandable resource for integration of data from many diverse proteomics experiments. Furthermore, we demonstrate that peptide identifications obtained from high-throughput proteomics can be integrated on a large scale with the human genome. This resource could serve as an expandable repository for MS-derived proteome information.
Desiere
Frank
F
Nestlé Research Center, 1000 Lausanne 26, Switzerland. fdesiere@yahoo.com <fdesiere@yahoo.com>
Deutsch
Eric W
EW
Nesvizhskii
Alexey I
AI
Mallick
Parag
P
King
Nichole L
NL
Eng
Jimmy K
JK
Aderem
Alan
A
Boyle
Rose
R
Brunner
Erich
E
Donohoe
Samuel
S
Fausto
Nelson
N
Hafen
Ernst
E
Hood
Lee
L
Katze
Michael G
MG
Kennedy
Kathleen A
KA
Kregenow
Floyd
F
Lee
Hookeun
H
Lin
Biaoyang
B
Martin
Dan
D
Ranish
Jeffrey A
JA
Rawlings
David J
DJ
Samelson
Lawrence E
LE
Shiio
Yuzuru
Y
Watts
Julian D
JD
Wollscheid
Bernd
B
Wright
Michael E
ME
Yan
Wei
W
Yang
Lihong
L
Yi
Eugene C
EC
Zhang
Hui
H
Aebersold
Ruedi
R
D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D030562:Databases, Protein; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D015894:Genome, Human; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000235:genetics*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012984:Software
D010455:Peptides; D020543:Proteome