title: Microarray-based resequencing of multiple Bacillus anthracis isolates.
abstract: We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 x 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 x 10(-6)). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.
authors.affiliation: Biological Defense Research Directorate, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA. mzwick@genetics.emory.edu
authors.forename: Michael E
authors.initials: ME
authors.lastname: Zwick
authors.forename: Farrell
authors.initials: F
authors.lastname: Mcafee
authors.forename: David J
authors.initials: DJ
authors.lastname: Cutler
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Read
authors.forename: Jacques
authors.initials: J
authors.lastname: Ravel
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Bowman
authors.forename: Darrell R
authors.initials: DR
authors.lastname: Galloway
authors.forename: Alfred
authors.initials: A
authors.lastname: Mateczun
mesh_terms: D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D002876:Chromosomes, Bacterial / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011995:Recombination, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000191:economics / Q000379:methods* / Q000592:standards; D013997:Time Factors