title: Ozone-induced oxidative stress response in Arabidopsis: transcription profiling by microarray approach.
abstract: High ozone concentration generates oxidative stress in plants. To investigate the detailed transcriptional regulation of Arabidopsis thaliana genes encoding antioxidant enzymes upon ozone stress, we performed a microarray analysis using Affymetrix GeneChip technology. Our transcription profiling revealed a differential expression equal or greater than 2-fold change for 2385 genes (at confidence 99%) in response to 350 ppb ozone dose after 3 and 6 hours of treatment. Among these, we chose 38 genes to be oxidative stress related in ozone treatment: 29 of them were 2 times up-regulated and 9 were shown to be down-regulated in at least one of the time points. Our study revealed a new transcription pattern for catalase genes and showed the first detailed transcriptional analysis of phenylopropanoid-related genes in ozone stress conditions.
authors.affiliation: Department of Biotechnology, Adam Mickiewicz University of Poznań, Miedzychodzka 5, 60-371 Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ludwikow
authors.forename: Patrick
authors.initials: P
authors.lastname: Gallois
authors.forename: Jan
authors.initials: J
authors.lastname: Sadowski
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010126:Ozone; D020005:Propanols / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D020005:Propanols; D018749:RNA, Plant; C439395:1-phenylpropanol; D010126:Ozone; D002374:Catalase