title: Development of an accelerated low-pH reversed-phase liquid chromatography column stability test.
abstract: The important experimental design criteria for an accelerated low-pH RPLC column stability test are discussed. The influence of method variables on the amount and rate of retention-loss and the final optimized parameters for the accelerated low-pH RPLC stability test are presented. The retention-loss curves for selected C8 and C18 stationary phases are compared. These studies indicate that ligand chain length, functionality and bonding density play an important role in determining the low-pH stability of a stationary phase. Additionally, elemental analysis data are used to infer the mechanism responsible for the observed retention-loss under low-pH conditions.
authors.affiliation: Waters Corporation, 34 Maple Street, Milford, MA 01757, USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Trammell
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Boissel
authors.forename: Christina
authors.initials: C
authors.lastname: Carignan
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: O'Shea
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hudalla
authors.forename: Uwe D
authors.initials: UD
authors.lastname: Neue
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Iraneta
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D013696:Temperature