Production of a monoclonal antibody to canine thymus and activation-regulated chemokine (TARC) and detection of TARC in lesional skin from dogs with atopic dermatitis.
A monoclonal antibody to canine thymus and activation-regulated chemokine (TARC/CCL17) was developed to examine the association of TARC with the immunopathogenesis of canine atopic dermatitis (AD). Recombinant canine TARC was prepared using an E. coli expression system. Results of transwell chemotaxis assay demonstrated that the recombinant canine TARC showed chemotactic activity for canine lymphoid cells expressing CC chemokine receptor 4 (CCR4). Mice were then immunized with the recombinant canine TARC to obtain monoclonal antibodies. Among the monoclonal antibodies thereby obtained, one monoclonal antibody (CTA-1) was found to react with both recombinant and authentic canine TARC in ELISA and flowcytometric assays, respectively. Immunohistochemical analysis using the monoclonal antibody CTA-1 demonstrated that keratinocytes were major TARC producing cells in lesional skin of dogs with AD.
Maeda
Sadatoshi
S
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Tsukui
Toshihiro
T
Saze
Koh-ichi
K
Masuda
Kenichi
K
Ohno
Koichi
K
Tsujimoto
Hajime
H
Iwabuchi
Shigehiro
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D054414:Chemokine CCL17; D019742:Chemokines, CC / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology; D002630:Chemotactic Factors / Q000494:pharmacology; D003876:Dermatitis, Atopic / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000209:etiology / Q000276:immunology*; D004285:Dogs; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D012867:Skin / Q000737:chemistry*
D000911:Antibodies, Monoclonal; C106230:CCL17 protein, human; C499538:Ccl17 protein, mouse; D054414:Chemokine CCL17; D019742:Chemokines, CC; D002630:Chemotactic Factors; D011994:Recombinant Proteins