[In vitro chemosensitivity test for hepatocellular carcinoma using collagen-gel droplet embedded cultures].
This study evaluated an in vitro assay for chemosensitivity test using a collagen-gel droplet-embedded culture drug sensitivity test (CD-DST) for hepatocellular carcinoma (HCC). In 25 patients with HCC, in vitro chemosensitivity to 5-fluorouracil (5-FU), epirubicin (EPI), and cisplatin (CDDP) was examined by CD-DST, and 5-FU, EPI, and paclitaxel (PTX) were examined in 38 patients with breast cancer. Successful rates of chemosensitive evaluation by CD-DST were 64% for HCC and 79% for breast cancers. Although chemosensitivities of breast cancer were 5-FU 23.1%, EPI 83.3%, and PTX 67.7%, only one HCC sample was sensitive to EPI. Growth rates of HCC for 7 days of culture were significantly lower than those of breast cancers (1.04 vs 3.61). The culture methods for HCC in CD-DST should be improved to estimate accurate results.
Nakagawa
Takahito
T
Dept of General Surgery, Hokkaido University, Graduate School of Medicine,
Takahashi
Hiromasa
H
Kamiyama
Toshiya
T
Nakanishi
Kazuaki
K
Takahashi
Masato
M
Watanabe
Ken-ichi
K
Taguchi
Kazunori
K
Tsukahara
Miyuki
M
Nakajima
Haruhiko
H
Kamachi
Hirofumi
H
Kurauchi
Nobuaki
N
Kamiyama
Naoya
N
Khor
Lee-Wee
LW
Abumiya
Kaori
K
Matsushita
Michiaki
M
Kobayashi
Hisayuki
H
Todo
Satoru
S
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002945:Cisplatin / Q000494:pharmacology; D003094:Collagen; D003470:Culture Media; D004354:Drug Screening Assays, Antitumor / Q000379:methods* / Q000592:standards; D015251:Epirubicin / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D005782:Gels; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology; D016610:Tissue Embedding
D000970:Antineoplastic Agents; D003470:Culture Media; D005782:Gels; D015251:Epirubicin; D003094:Collagen; D017239:Paclitaxel; D002945:Cisplatin; D005472:Fluorouracil