DNA-directed in vitro synthesis of elongation factor Tu.
The in vitro synthesis of EF-Tu was studied using the transducing phage lambdarifd18 DNA as template. The EF-Tu synthesized was identified by its immunological properties, gel analysis, and its ability to interact with GDP and EF-Ts. Two other unidentified polypeptides were also precipitated from the incubation mixture by EF-Tu antiserum. The synthesis of total EF-Tu immunoprecipitable material was depressed about one-half by guanosine-5'-diphosphate-3'-diphosphate which was shown to inhibit the transcription process.
Chu
F
F
Department of Biochemistry, Roche Institute of Molecular Biology, Nutley, New Jersey 07110, USA.
Miller
D L
DL
Schulz
T
T
Weissbach
H
H
Brot
N
N
D000906:Antibodies / Q000276:immunology; D002474:Cell-Free System; D002850:Chromatography, Gel; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006153:Guanosine Diphosphate / Q000378:metabolism; D006159:Guanosine Tetraphosphate / Q000494:pharmacology; D047468:Immunoprecipitation; D007700:Kinetics; D010444:Peptide Elongation Factor Tu / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000187:drug effects; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects
D000906:Antibodies; D004269:DNA, Bacterial; D010455:Peptides; D006153:Guanosine Diphosphate; D006159:Guanosine Tetraphosphate; D010444:Peptide Elongation Factor Tu