The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots.
Local accumulation of the plant growth regulator auxin mediates pattern formation in Arabidopsis roots and influences outgrowth and development of lateral root- and shoot-derived primordia. However, it has remained unclear how auxin can simultaneously regulate patterning and organ outgrowth and how its distribution is stabilized in a primordium-specific manner. Here we show that five PIN genes collectively control auxin distribution to regulate cell division and cell expansion in the primary root. Furthermore, the joint action of these genes has an important role in pattern formation by focusing the auxin maximum and restricting the expression domain of PLETHORA (PLT) genes, major determinants for root stem cell specification. In turn, PLT genes are required for PIN gene transcription to stabilize the auxin maximum at the distal root tip. Our data reveal an interaction network of auxin transport facilitators and root fate determinants that control patterning and growth of the root primordium.
Blilou
Ikram
I
Department of Molecular Genetics, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands.
Xu
Jian
J
Wildwater
Marjolein
M
Willemsen
Viola
V
Paponov
Ivan
I
Friml
Jirí
J
Heidstra
Renze
R
Aida
Mitsuhiro
M
Palme
Klaus
K
Scheres
Ben
B
D017360:Arabidopsis / Q000166:cytology / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019521:Body Patterning; D002455:Cell Division; D049108:Cell Enlargement; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D018517:Plant Roots / Q000166:cytology / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C116363:PIN1 protein, Arabidopsis; C493246:PLT1 protein, Arabidopsis; D012333:RNA, Messenger; D014157:Transcription Factors