[Effects of nitric oxide on root growth and its oxidative damage in wheat seedling under salt stress].
Effects of nitric oxide (NO), a substance newly found to have protective functions in plants, on root growth of wheat (Triticum aestivum L. Yangmai 158) seedlings under salt stress were studied. Sodium nitroprusside (SNP), an NO donor, markedly alleviated the inhibitory effect of salt on root elongation at salt concentrations around 150 mmol/L, but was ineffective when NaCl concentration was at 300 mmol/L or higher. It was most effective at 0.05-0.1 mmol/L, and had harmful effect at 0.30-5 mmol/L. Furthermore, when wheat seedling roots were treated with 150 mmol/L NaCl in combination with 0.05 mmol/L SNP and NO scavenger (hemoglobin, Hb), NO(-)(2)/NO(-)(3) and K(3)Fe(CN)(6) as controls, both of which were the two other major products besides NO when SNP dissolved in water, respectively, activities of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) were enhanced to some extent. While, the generation of O(-.)(2) and the accumulation of MDA and H(2)O(2) were alleviated. DNA laddering was observed when wheat seedling roots exposed to 150 mmol/L NaCl for 6 d, also suggesting that salt stress might induce oxidative damage in root-tips. In combination with hemoglobin, NO(-)(2)/NO(-)(3) and K(3)Fe(CN)(6) as controls, the results showed that NO could block DNA ladders. Above results suggest that it is NO rather than any substances which is protecting root-tip cells of wheat seedlings from oxidative damage caused by salt stress.
Chen
Ming
M
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Shen
Wen-Biao
WB
Ruan
Hai-Hua
HH
Xu
Lang-Lai
LL
D017209:Apoptosis; D053938:DNA Fragmentation; D006861:Hydrogen Peroxide / Q000032:analysis; D008315:Malondialdehyde / Q000032:analysis; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development* / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D014908:Triticum / Q000254:growth & development* / Q000378:metabolism
D013481:Superoxides; D009599:Nitroprusside; D009569:Nitric Oxide; D012965:Sodium Chloride; D008315:Malondialdehyde; D006861:Hydrogen Peroxide