Immunohistochemical analysis of S6K1 and S6K2 expression in endometrial adenocarcinomas.
OBJECTIVE
The aim of this study was to analyze the levels 70 kDa ribosomal protein S6 kinase 1 (S6K1) and 70 kDa ribosomal protein S6 kinase 2 (S6K2) expression and S6 ribosomal protein phosphorylation in endometrial adenocarcinomas.


METHODS
S6K1/2 expression and phosphorylated ribosomal S6 protein (phS6) content have been detected in formalin fixed, paraffin embedded sections of 50 human endometrial adenocarcinomas with different grade of differentiation and in 13 normal endometrial tissues using immunohistochemical approach with following semiquantitative analysis.


RESULTS
In normal endometrial epithelial cells both S6K1 and S6K2 were expressed on the low level. S6K1 and S6K2 has been detected predominantly in stromal elements. Increased phS6 level was found in superficial epithelial cells. In deeper parts of endometrial glands and vessels phS6 was discovered occasionally. In endometrial adenocarcinoma's tissues, overexpression of S6K1 was found in cytoplasm and nuclei in 8.0% of cases, overexpression of S6K2--in cytoplasm in 12.0% of cases and in nuclei in 18.0% of cases. Overexpression of S6K1 in endothelial cells of vessels was discovered in 58% of cases. Positive correlation has been determined between: 1) tumor stage and intensity of stromal staining for S6K1 (p = 0.027); 2) tumor differentiation grade and intensity of cytoplasm staining of cancer cells for S6K1 (p = 0.039); 3) intensity of stromal staining and vessel's staining for S6K1 (p = 0.019); 4) vessel's staining for S6K1 and staining for phS6 (p = 0.028).


CONCLUSIONS
Overexpression of S6K1 and S6K2 is a characteristic feature of parenchyma and vessels of endometrial adenocarcinomas. Phosphorylation of ribosomal S6 protein is not dependent from expression level of S6K1 and S6K2.
Lyzogubov
Valeriy V
VV
Morphological Laboratory Biontec, Dnipropetrovsk, Ukraine.
Lytvyn
Dmytro I
DI
Dudchenko
Taras M
TM
Lubchenko
Natalia V
NV
Pogrybniy
Petro V
PV
Nespryadko
Sergij V
SV
Vinnitska
Alla B
AB
Usenko
Vasiliy S
VS
Gout
Ivan T
IT
Filonenko
Valeriy V
VV
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010766:Phosphorylation; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000096:biosynthesis* / Q000235:genetics*; D015854:Up-Regulation
D038762:Ribosomal Protein S6 Kinases, 70-kDa