Genetic transformation of Glaphimia glauca by Agrobacterium rhizogenes and the production of norfriedelanes.
Transformed root cultures of Galphimia glauca (Malpighiaceae) were established by infecting cotyledons and hypocotyls with Agrobacterium rhizogenes ATCC 15 834. Cotyledon-derived cell lines were grown in liquid B5 nutrient medium without phytohormones and have shown the typical hairy roots phenotype over two years of continuous subculturing. PCR analysis was used to confirm the integration of rol A and rol C genes into the plant genome. The transformed cultures synthesized three major norfriedelanes, the new glaucacetalins A-C (1-3), which were secreted into the nutrient medium. The structural elucidation of these in vitro produced metabolites was performed by the application of high resolution NMR techniques that proved them to be triterpenoids related to the known galphimines, the sedative principles of this plant species. These results suggest the possibility of further biotechnological exploration of sedative friedelane biosynthesis by in vitro plant organ cultures.
Náder
Blanca L
BL
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
Cardoso Taketa
Alexandre T
AT
Iturriaga
Gabriel
G
Pereda-Miranda
Rogelio
R
Villarreal
Ma Luisa
ML
D018929:Cell Culture Techniques; D031582:Galphimia / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008517:Phytotherapy; D018517:Plant Roots; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal / Q000235:genetics* / Q000378:metabolism; D012231:Rhizobium; D014315:Triterpenes / Q000378:metabolism*
D014315:Triterpenes; C421805:friedelane