[Effects of Eupolyphaga Sinensis Walker on erythrocyte's CR1 activity and anti-cardiolipin antibody level in rats with stagnation of blood].
OBJECTIVE
To study the effect of Eupolyphaga Sinensis Walker(ESW) on red blood cell immune adherence (RCIA) and serum anticardiolipin antibody level in rat model of Yin-deficiency Huo-excess with chronic blood stasis.


METHODS
The model was made by i.m. injection of dexamethasone (0.5 mg/kg) and adrenaline (0.84 mg/kg). The serum anti-cardiolipin antibodies (ACA) ACA-IgG, ACA-IgA and ACA-IgM and the plasma D-dimmer levels were measured by using enzyme-linked immunosorbent assay (ELISA). The body and organ weight of the rats were measured.


RESULTS
For rats of Yin-deficiency Huo-excess, rosette rates of red blood cell C3b receptors (RBC-C3b RR) and red blood cell cancer (RBC-CaR) decreased, the serum ACA-IgG, ACA-IgA, ACA-IgM and the plasma D-dimmer levels markedly increased, body weight and the weight of spleen and thymus all decreased. ESW (5 mg/kg, 10 mg/kg) increased RBC-C3bRR and RBC-CaR, reduced serum ACA-IgG, ACA-IgA, ACA-IgM and plasma D-dimmer levels, and increased spleen weight of the rats.


CONCLUSIONS
ESW can boost the immune function in rats of Yin-deficiency Huo-excess with chronic blood stasis.
Yang
Yao-Fang
YF
Research Room of Pharmacology, Shanghai Fifth Peopleos Hospital Affiliated to Fudan University, Shanghai 200240, China.
Wang
Sai-Qian
SQ
Feng
Mei-Jia
MJ
Ning
Ai-Hua
AH
Huang
Chang-Hui
CH
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000378:metabolism; D001835:Body Weight / Q000276:immunology; D002308:Cardiolipins / Q000276:immunology*; D003058:Cockroaches / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000276:immunology; D051381:Rats; D017463:Receptors, Complement 3b / Q000378:metabolism*; D016710:Yin Deficiency / Q000276:immunology / Q000473:pathology
D000906:Antibodies; D002308:Cardiolipins; D005338:Fibrin Fibrinogen Degradation Products; D017463:Receptors, Complement 3b; C036309:fibrin fragment D