Expression of ST13 in colorectal cancer and adjacent normal tissues.
OBJECTIVE
To investigate the in situ expression of suppression of tumorigenecity 13 (ST13) mRNA in both colorectal cancer and adjacent normal tissues.


METHODS
Colorectal cancer cell lines SW1116, SW620 and CoLo205 were enrolled to confirm the feasibility of the in situ hybridization procedure. Seven colorectal cancer and adjacent normal tissues were included for RNA-RNA in situ hybridization.


RESULTS
The expression of ST13 in the seven normal colon tissues was positive and the positive signals appeared in mucosal cells. Only three of the seven colorectal cancer tissues had positive hybridization signals that appeared in adenocarcinoma cells.


CONCLUSIONS
The expression of ST13 decreases in colorectal cancer tissue compared with that in adjacent normal tissue. ST13 is mostly expressed in colorectal epithelia and adenocarcinoma cells.
Wang
Lin-Bo
LB
Department of Surgical Oncology, Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou 310016, Zhejiang Province, China. wanglinbo@medmail.com.cn
Zheng
Shu
S
Zhang
Su-Zhan
SZ
Peng
Jia-Ping
JP
Ye
Feng
F
Fang
Shi-Chang
SC
Wu
Jin-Min
JM
D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D003106:Colon / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D005240:Feasibility Studies; D006801:Humans; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism*; D008875:Middle Aged; D012007:Rectum / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D018832:Molecular Chaperones
D002352:Carrier Proteins; D025521:Tumor Suppressor Proteins; C101119:ST13 protein, human; D018832:Molecular Chaperones