title: [Endothelial nitric oxide synthase gene transfected endothelial cells inhibit smooth muscle cell proliferation and platelet aggregation early in vitro].
abstract: OBJECTIVE
  To test the function of endothelial cells transfected eNOS gene.
  
  
  METHODS
  To test the function of eNOS-overexpressing endothelial cells inhibiting smooth muscle cell proliferation and platelet aggregation in vitro by colorimetry, MTT and (3)H-TdR permeate, respectively, in four different endothelial cells groups.
  
  
  RESULTS
  The platelet aggregation is significantly decreasing between the groups of EC and eNOS at the different time (P < 0.05), is 42.2 and 32.6 separately at the time of 120 h. The SMC proliferation is significantly decreasing between the groups of EC and eNOS at the different time (P < 0.05). It is 0.28 and 0.22 by MTT test, 4691 and 3995 by (3)H-TdR permeate separately at the time of 120 h.
  
  
  CONCLUSIONS
  eNOS-overexpressing endothelial cells inhibited significantly smooth muscle cells proliferation and platelet aggregation in vitro; which shows powerful effect 48 hours post transfection and lasts up to 120 hours at the least; and were held back by L-NAME.
authors.affiliation: Department of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Xue
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Bai-Gen
authors.initials: BG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002450:Cell Communication; D049109:Cell Proliferation; D018920:Coculture Techniques; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D009569:Nitric Oxide / Q000378:metabolism* / Q000502:physiology; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D010974:Platelet Aggregation / Q000502:physiology*; D014162:Transfection
chemical_list: D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III