title: First isolation of metallo-beta-lactamase-producing multiresistant Klebsiella pneumoniae from a patient in Brazil.
abstract: A multiresistant Klebsiella pneumoniae isolate was taken from the blood of a 75-year-old patient with nosocomial pneumonia who developed septic shock and failed therapy with imipenem. The isolate presented an MIC of imipenem of 128 microg/ml, and the production of a metallo-beta-lactamase was confirmed by phenotypic and genotypic techniques. We here report, for the first time, the detection of a metalloenzyme (IMP-1)-producing K. pneumoniae clinical strain in Latin America. The gene responsible for this phenotype was found to be bla(IMP-1), carried in a class 1 integron.
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Avenida Professor Lineu Prestes 580, Butantã, CEP 05508-900, São Paulo SP, Brazil. lincopan@usp.br
authors.forename: Nilton
authors.initials: N
authors.lastname: Lincopan
authors.forename: John Anthony
authors.initials: JA
authors.lastname: McCulloch
authors.forename: Cristina
authors.initials: C
authors.lastname: Reinert
authors.forename: Valéria C
authors.initials: VC
authors.lastname: Cassettari
authors.forename: Ana C
authors.initials: AC
authors.lastname: Gales
authors.forename: Elsa M
authors.initials: EM
authors.lastname: Mamizuka
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D024901:Drug Resistance, Multiple, Bacterial; D017809:Fatal Outcome; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases