Influence of different pneumoperitoneal pressures on tumor cell distribution in rats.
BACKGROUND
The effect of different pneumoperitoneal pressures on tumor cell distribution was investigated.


METHODS
Donryu rats were allocated to receive carbon dioxide pneumoperitoneum at 5, 10, or 15 mmHg for 60 min or to serve as a control. During the procedure, each rat was inoculated with radiolabeled ascites hepatoma cells via the portal vein (experiment 1) or femoral vein (experiment 2). In both experiments, the rats were killed 30, 60, 90, or 120 min after tumor cell inoculation, and the liver and lungs were extirpated for radioactivity count (n = 5 or 6 for each time point in each group).


RESULTS
In experiment 1, the percentage of injected dose (% ID) for the liver was greater than for the other three groups 120 min after tumor cell inoculation. There were no significant differences in the %IDs of the lungs at any time point among the groups. In experiment 2, there were no significant differences in the %IDs of the liver and lungs at any time point among the groups.


CONCLUSIONS
These results suggest that an elevated insufflation pressure facilitates the location of intraportally injected tumor cells in the liver, and that pulmonary location of the tumor cells may not depend on insufflation pressures in this animal model.
Nakada
H
H
Department of Surgery, Saitama Medical Center, Saitama Medical School, 1981 Kamoda Kawagoe, Saitama 350-8550, Japan.
Ishida
H
H
Hashimoto
D
D
Mori
T
T
Hosono
M
M
D000818:Animals; D001201:Ascites; D002245:Carbon Dioxide / Q000008:administration & dosage; D002452:Cell Count; D004198:Disease Susceptibility; D005268:Femoral Vein; D007275:Injections, Intravenous; D007327:Insufflation; D008114:Liver Neoplasms, Experimental / Q000556:secondary*; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D009368:Neoplasm Transplantation; D009360:Neoplastic Cells, Circulating; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011169:Portal Vein; D011312:Pressure; D051381:Rats
D002245:Carbon Dioxide