title: Conformational changes in the ligand-binding domain of a functional ionotropic glutamate receptor.
abstract: Fluorescence resonance energy transfer was used to determine the structural changes in the extracellular ligand-binding segment in a functional glutamate receptor that contains the ligand-binding, transmembrane, and C-terminal segments. These studies indicate that the structural changes previously reported for the isolated ligand-binding domain due to the binding of partial and full agonists are also observed in this functional receptor, thus validating the detailed structure-function relationships that have been previously developed based on the structure of the isolated ligand-binding domain. Additionally, these studies provide the first evidence that there are no significant changes in the extent of cleft closure between the activated and desensitized states of the glutamate bound form of the receptor consistent with the previous functional investigations, which suggest that desensitization is mediated primarily by changes in the interactions between subunits composing the receptor.
authors.affiliation: Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, Texas 77030, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Du
authors.forename: Scott A
authors.initials: SA
authors.lastname: Reid
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Jayaraman
mesh_terms: D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D002460:Cell Line; D018360:Crystallography, X-Ray; D006801:Humans; D007608:Kainic Acid / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D018091:Receptors, AMPA / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D013050:Spectrometry, Fluorescence; D014162:Transfection
chemical_list: D001059:Apoproteins; D008024:Ligands; D018091:Receptors, AMPA; D011993:Recombinant Fusion Proteins; C104724:glutamate receptor ionotropic, AMPA 4; D007608:Kainic Acid