A genome wide screening approach for membrane-targeted proteins.
Membrane-associated proteins are critical for intra- and intercellular communication. Accordingly approaches are needed for rapid and comprehensive identification of all membrane-targeted gene products in a given cell or tissue. Here we describe a modification of the yeast Ras recruitment system to this end and designate the modified approach the Ras membrane trap (RMT). A pilot RMT screen was carried out on the central nervous system of the mollusk Lymnaea stagnalis, a model organism from a phylum that still lacks a representative with a sequenced genome. 112 gene products were identified in the screen of which 79 lack assignable homologs in available data bases. Currently available annotation tools predicted membrane association of only 45% of the 112 proteins, although experimental verification in mammalian cells confirmed membrane association for all clones tested. Thus, genome annotation using currently available tools is likely to underpredict representation of membrane-associated gene products. The 32 proteins with known homologies include many targeted to the endoplasmic reticulum or the nucleus, thus RMT provides a tool that can cover intracellular membrane proteomes. Two sequences were found to represent gene families not found to date in invertebrate genomes, emphasizing the need for whole genome sequences from mollusks and indeed from representatives of all major invertebrate phyla.
Jaaro
Hanna
H
Department of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel.
Levy
Zehava
Z
Fainzilber
Mike
M
D000818:Animals; D019556:COS Cells; D002490:Central Nervous System / Q000737:chemistry; D002522:Chlorocebus aethiops; D019295:Computational Biology; D015723:Gene Library; D016678:Genome; D008195:Lymnaea / Q000737:chemistry* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D018631:ras Proteins / Q000235:genetics
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome; D018631:ras Proteins