Constructs and methods for hairpin RNA-mediated gene silencing in plants.
Double-stranded RNA (dsRNA) induces an endogenous sequence-specific RNA degradation mechanism in most eukaryotic cells. The mechanism can be harnessed to silence genes in plants by expressing self-complementary single-stranded (hairpin) RNA in which the duplexed region has the same sequence as part of the target gene's mRNA. We describe a number of plasmid vectors for generating hairpin RNAs, including those designed for high-throughput cloning, and provide protocols for their use.
Helliwell
Chris A
CA
CSIRO Plant Industry, Canberra ACT 2601, Australia.
Waterhouse
Peter M
PM
D001483:Base Sequence; D017931:DNA Primers; D020868:Gene Silencing / Q000502:physiology*; D005822:Genetic Vectors; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D018749:RNA, Plant / Q000737:chemistry / Q000502:physiology*
D017931:DNA Primers; D018749:RNA, Plant