title: [The clinical study on application of using a novel blockade technique for gastric cancer to decrease blood-borne metastasis of cancer cells].
abstract: OBJECTIVE
  To evaluate the effect of a novel blockade technique for gastric cancer on blood-borne metastasis of gastric cancer cells to portal vein.
  
  
  METHODS
  Twenty-three cases of gastric cancer were divided into routine operation group (8 cases intraoperatively without blockade technique) and blockade group (15 cases with blockade technique). Blood samples from portal vein pre- and intraoperatively, as well as gastroepiploic vein limited within the blockade area were obtained to detect CK19 mRNA expression by using RT-PCR technique.
  
  
  RESULTS
  Before the dissection of gastric lesion, the overall positive rate of CK19 mRNA expression in portal vein blood is 34.7% (9/23), including 37.5% (3/8) in routine operation group and 33.3% (5/15) in blockade group. While the course of tumor resection, those positive rates were 87.5% (7/8) in routine operation group and 6.7% (1/15) in blockade group respectively (P < 0.05). CK19 mRNA expression in the right gastroepiploic venous blood limited within the blocking area was all positive in 15 cases of blockade group.
  
  
  CONCLUSIONS
  This blockade technique can be used effectively to block the intraoperative spread of gastric cancer cells, thus prevent blood-borne metastasis due to operative manipulation.
authors.affiliation: Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Huang
authors.forename: Qun-Hua
authors.initials: QH
authors.lastname: Zhang
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Gan
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.forename: Quan-Xing
authors.initials: QX
authors.lastname: Ni
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D005260:Female; D005743:Gastrectomy; D006801:Humans; D007633:Keratins / Q000097:blood / Q000235:genetics; D008026:Ligation; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000517:prevention & control; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000097:blood / Q000473:pathology* / Q000601:surgery*; D014656:Vascular Surgical Procedures / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D007633:Keratins