title: Expression of the LXRalpha protein in human atherosclerotic lesions.
abstract: OBJECTIVE
  Liver X-activated receptor alpha (LXRalpha) regulates multiple genes controlling cholesterol metabolism and transport. To clarify its role in atherogenesis, we established a monoclonal antibody recognizing native human LXRalpha protein and studied the expression pattern in human atherosclerotic lesions.
  
  
  RESULTS
  A novel monoclonal antibody PPZ0412 was raised against the ligand-binding domain of LXRalpha, which can be used for immunostaining of human LXRalpha protein. LXRalpha protein was detected in the nucleus of macrophages in the liver, spleen, or lung and also in hepatocytes and adipocytes. In atherosclerotic lesions, the LXRalpha protein was detected in macrophages positive for scavenger receptor class A and/or CD68.
  
  
  CONCLUSIONS
  In the human body, the LXRalpha protein is highly expressed in macrophage lineage cells and foam cells in atherosclerotic lesions and is identified as a target for intervention in atherosclerotic disease.
authors.affiliation: Department of Systems Biology and Medicine, Research Center for Advanced Science and Technology, the University of Tokyo, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Shuying
authors.initials: S
authors.lastname: Jiang
authors.forename: Wakako
authors.initials: W
authors.lastname: Takabe
authors.forename: Riuko
authors.initials: R
authors.lastname: Ohashi
authors.forename: Toshiya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Katsumi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iwanari
authors.forename: Noriko
authors.initials: N
authors.lastname: Noguchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Naito
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001161:Arteriosclerosis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology*; D019556:COS Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005487:Foam Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008099:Liver / Q000378:metabolism; D000071518:Liver X Receptors; D008168:Lung / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D057093:Orphan Nuclear Receptors; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D000071518:Liver X Receptors; C000605213:NR1H3 protein, human; C000605214:Nr1h3 protein, mouse; D057093:Orphan Nuclear Receptors; D018160:Receptors, Cytoplasmic and Nuclear