title: Structural characterization of the O-polysaccharide antigen of Edwardsiella tarda MT 108.
abstract: Edwardsiella tarda, a Gram-negative bacterium, is an important cause of hemorrhagic septicemia in fish and also of gastro- and extraintestinal infections in humans. The lipopolysaccharide produced by the fish pathogenic strain E. tarda MT 108 was isolated and the structure of its antigenic O-polysaccharide component determined by the application of chemical analyses, high-resolution 1D and 2D nuclear magnetic resonance spectroscopy, and mass spectrometry. The polysaccharide was found to be a polymer of a repeating pentasaccharide unit composed of 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 2-acetamido-2-deoxy-D-galactose (D-GalNAc), D-galactose (D-Gal), L-rhamnose (L-Rha), D-galacturonic acid (D-GalA) and (2S,3R)-threonine (1:1:1:1:1:1) having the structure: [structure: see text].
authors.affiliation: Institute for Biological Sciences, National Research Council, Ottawa, ON, K1A OR6, Canada.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Ludmila
authors.initials: L
authors.lastname: Nossova
authors.forename: Malcolm B
authors.initials: MB
authors.lastname: Perry
authors.forename: William W
authors.initials: WW
authors.lastname: Kay
mesh_terms: D002240:Carbohydrate Sequence; D020609:Edwardsiella tarda / Q000737:chemistry* / Q000145:classification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*
chemical_list: D019081:O Antigens