title: FIZICS: fluorescent imaging zone identification system, a novel macro imaging system.
abstract: Constantly improving biological assay development continues to drive technological requirements. Recently, a specification was defined for capturing white light and fluorescent images of agar plates ranging in size from the NUNC Omni tray (96-well footprint, 128 x 85 mm) to the NUNC Bio Assay Dish (245 x 245 mm). An evaluation of commercially available products failed to identify any system capable of fluorescent macroimaging with discrete wavelength selection. To address the lack of a commercially available system, a custom imaging system was designed and constructed. This system provides the same capabilities of many commercially available systems with the added ability to fluorescently image up to a 245 x 245 mm area using wavelengths in the visible light spectrum.
authors.affiliation: P.O. Box 2000, Merck Research Laboratories, Rahway, NJ 07065, USA. stephen_skwish@merck.com
authors.forename: Stephen
authors.initials: S
authors.lastname: Skwish
authors.forename: Francisco
authors.initials: F
authors.lastname: Asensio
authors.forename: Greg
authors.initials: G
authors.lastname: King
authors.forename: Glenn
authors.initials: G
authors.lastname: Clarke
authors.forename: Gary
authors.initials: G
authors.lastname: Kath
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Salvatore
authors.forename: Claude
authors.initials: C
authors.lastname: Dufresne
mesh_terms: D001681:Biological Assay / Q000295:instrumentation; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D012984:Software