Effect of granulocyte colony-stimulating factor on opsonin receptor expression in cerulein-induced acute pancreatitis.
OBJECTIVE
Decreased levels of expression of opsonin receptors (CD11b and CD32/16) on peritoneal exudate neutrophils may lead to susceptibility to infection. Granulocyte colony-stimulating factor (G-CSF) increases the expression levels of CD11b on neutrophils and prolongs neutrophil survival. The effects of G-CSF on neutrophils and opsonin receptor expressions of neutrophils were investigated in cerulein-induced acute pancreatitis.


METHODS
Forty-two mice were randomly assigned to each group (n = 6). Mice received subcutaneous G-CSF (120 microg/kg body weight) before the induction of acute pancreatitis with cerulein. Saline was used for instead of G-CSF or cerulein solution in control groups. CD11b and CD32/16 expression levels on circulatory and peritoneal exudate neutrophils were investigated 6 and 24 hours after the induction of acute pancreatitis.


RESULTS
Treatment with G-CSF did not aggravate the inflammation of pancreatic tissue evaluated by plasma amylase, acinar necrosis. However, it significantly increased the number of peritoneal exudate neutrophils (P < 0.05) and the CD11b- (P < 0.05) and CD32/16-positive (P < 0.05) peritoneal exudate neutrophils in mice with cerulein-induced acute pancreatitis. The means of fluorescence intensity for CD11b and CD32/16 expressions on circulatory and peritoneal exudate neutrophils were also elevated in the G-CSF groups.


CONCLUSIONS
G-CSF administration increases the numbers of neutrophils and improves expression levels of opsonin receptors on neutrophils in mice with cerulein-induced acute pancreatitis.
Tuo
Hongfang
H
First Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan. tuohongfang@yahoo.co.jp
Sugiyama
Masanori
M
Nakashima
Masanobu
M
Abe
Nobutsugu
N
Watanabe
Takashi
T
Atomi
Yutaka
Y
D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D002108:Ceruletide; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007958:Leukocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010195:Pancreatitis / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D017452:Receptors, IgG / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*
D039481:CD11b Antigen; D017452:Receptors, IgG; D011971:Receptors, Immunologic; C091196:opsonin receptor; D016179:Granulocyte Colony-Stimulating Factor; D002108:Ceruletide; D000681:Amylases