title: An evaluation of the role of antibodies to Actinobacillus pleuropneumoniae serovar 1 and 15 in the protection provided by sub-unit and live streptomycin-dependent pleuropneumonia vaccines.
abstract: OBJECTIVE
  To evaluate the serological response of pigs receiving either the Porcilis APP vaccine or a modified live vaccine based on a streptomycin-dependent (SD) strain of Actinobacillus pleuropneumoniae, and then challenged with an Australian isolate of A. pleuropneumoniae of either serovar 1 or 15 as a means of understanding the protection provided by both vaccines against serovar 1 but not against serovar 15.
  
  
  METHODS
  The serological tests evaluated were serovar-specific polysaccharide ELISA tests (for serovar 1 and 15), ELISA tests for antibodies to three A. pleuropneumoniae toxins (ApxI, ApxII and ApxIII) as well as to a 42 kDa outer membrane protein (OMP), a haemolysin neutralisation (HN) assay and immunoblotting. The tests were used to detect antibodies in vaccinated pigs that had been shown to be protected against serovar 1 but not serovar 15.
  
  
  RESULTS
  In the polysaccharide antigen ELISA assays, both vaccines resulted in a significant rise in the titre in the serovar 1 ELISA but not the serovar 15 ELISA. The Porcilis APP vaccinated pigs showed a significant response in the ApxI, ApxIII and 42 kDa OMP ELISA. In the ApxII ELISA, all pigs tested (the Porcilis APP vaccinates and the controls) were positive on entry to the trial. In the HN assay, the Porcilis APP vaccinated pigs showed a significant response after one dose while the SD vaccinated pigs required two doses of vaccine before a marked rise in titre was induced. Immunoblotting revealed that neither vaccine generated antibodies that recognised the ApxIII produced by serovar 15.
  
  
  CONCLUSIONS
  The failure of these vaccines to provide protection against serovar 15 may be due to novel virulence factors possessed by serovar 15, significant differences between the ApxIII toxin of serovar 15 and those present in the Porcilis APP vaccine or failure by both vaccines to induce antibodies to the serovar 15 specific polysaccharide.
authors.affiliation: Agency for Food and Fibre Sciences, Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Queensland 4105. yellowcocka2@yahoo.com
authors.forename: J Q
authors.initials: JQ
authors.lastname: Tumamao
authors.forename: R E
authors.initials: RE
authors.lastname: Bowles
authors.forename: H
authors.initials: H
authors.lastname: van den Bosch
authors.forename: H L B M
authors.initials: HL
authors.lastname: Klaasen
authors.forename: B W
authors.initials: BW
authors.lastname: Fenwick
authors.forename: P J
authors.initials: PJ
authors.lastname: Blackall
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D011001:Pleuropneumonia / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D012703:Serotyping / Q000662:veterinary; D013307:Streptomycin / Q000494:pharmacology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D013307:Streptomycin