title: Cell activating capacity of 50 Hz magnetic fields to release reactive oxygen intermediates in human umbilical cord blood-derived monocytes and in Mono Mac 6 cells.
abstract: The aim of this study was to investigate the mechanism of cell activation induced by extremely low frequency magnetic fields (ELF-MF) (50 Hz) in human cells. We examined the production of free radicals in human umbilical cord blood-derived monocytes and in human Mono Mac 6 cells. The release of superoxide radical anions was analyzed using nitroblue tetrazolium chloride and the total of reactive oxygen species (ROS) was detected using dihydrorhodamine 123. Our results show a significant increase of superoxide radical anion production up-to 1.4 fold as well as an increase in ROS release up-to 1.2 fold upon exposure of monocytes to 1 mT ELF-MF (45 min). Mono Mac 6 cells exhibit higher superoxide radical anion and ROS production up-to 1.4 and 1.5 fold, respectively. These results indicate that Mono Mac 6 cells are more sensitive to ELF-MF than monocytes. Using diphenyleneiodonium chloride (DPI) a specific inhibitor for the NADPH oxidase, the MF-effect was not inhibited in Mono Mac 6 cells. Therefore, we suggest that ELF-MF exposure induces the activation of NADH oxidase in these cells. However, the MF-effect was inhibited by DPI in monocytes, indicating the activation of the NADPH oxidase after exposure to ELF-MF.
authors.affiliation: University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, Albert-Einstein-Str. 3, D-18059 Rostock, Germany.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Lupke
authors.forename: Jana
authors.initials: J
authors.lastname: Rollwitz
authors.forename: Myrtill
authors.initials: M
authors.lastname: Simkó
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005312:Fetal Blood; D005434:Flow Cytometry; D006801:Humans; D008070:Lipopolysaccharides; D009000:Monocytes / Q000378:metabolism / Q000528:radiation effects*; D009097:Multienzyme Complexes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009861:Onium Compounds / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate
chemical_list: D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D009097:Multienzyme Complexes; D009861:Onium Compounds; D017382:Reactive Oxygen Species; D013481:Superoxides; C007517:diphenyleneiodonium; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate