title: Lipid bilayer tethered inside a nanoporous support: a solid-state nuclear magnetic resonance investigation.
abstract: (31)P and (1)H solid-state nuclear magnetic resonance (NMR) experiments have been designed with the aim of studying directly the formation of supported bilayers tethered inside nanoporous aluminum oxide supports as a model of biomimetic membranes. The static and magic angle spinning (31)P NMR spectra of the supported bilayers have been compared with the experimental and simulated spectra of a simpler model with cylindrical geometry, namely a phospholipid bilayer adsorbed on an oriented polymer sheet. The broadening observed for the nanoporous model is most likely due to the presence of paramagnetic ions in the aluminum oxide. A phospholipid lateral diffusion coefficient of (2.8 +/- 0.4) x 10(-8) cm(2)/s has been measured for the tethered bilayer on a spherical support, indicating a good fluidity as compared with adsorbed membrane models.
authors.affiliation: Unité de Génie Enzymatique et Cellulaire, UMR 6022 du CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Wattraint
authors.forename: Alexandre
authors.initials: A
authors.lastname: Arnold
authors.forename: Michèle
authors.initials: M
authors.lastname: Auger
authors.forename: Christian
authors.initials: C
authors.lastname: Bourdillon
authors.forename: Catherine
authors.initials: C
authors.lastname: Sarazin
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D016062:Porosity
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids