title: Mutation of a LysR-type regulator of antifungal activity results in a growth advantage in stationary phase phenotype in Pseudomonas aureofaciens PA147-2.
abstract: The growth advantage in stationary phase (GASP) phenotype was shown to be present in two mutants lacking the antifungal phenotype (Af(-) mutants) of Pseudomonas aureofaciens PA147-2. Complementation demonstrated a correlation between GASP and the antifungal defect in one strain but not in the second. Sequence analysis revealed the Af(-) GASP strain had a mutation in a gene (finR) encoding a LysR-type regulator. Antifungal-minus mutants arose in starved cultures, and those aged cultures had increased fitness. Taken together, the results show that there are at least two paths to the GASP phenotype in P. aureofaciens, one of which results in a concomitant loss of the antifungal phenotype.
authors.affiliation: Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand. mark.silby@tufts.edu
authors.forename: Mark W
authors.initials: MW
authors.lastname: Silby
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Giddens
authors.forename: H Khris
authors.initials: HK
authors.lastname: Mahanty
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D010641:Phenotype; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D003470:Culture Media; D014157:Transcription Factors; C073470:LysR protein, Bacteria