Molecular cloning and sequence analysis of cDNAs encoding for Boophilus microplus, Haemaphysalis longicornis and Rhipicephalus appendiculatus actins.
The nucleotide and deduced amino acid sequences of the actins from ticks, Boophilus microplus, Haemaphysalis longicornis and Rhipicephalus appendiculatus, have been determined. Nucleotide sequence analysis showed open reading frames of 1128-nucleotide-long encoding proteins of 376 amino acids with a predicted molecular weight of 41.82 kDa each. Comparison between the nucleic acid and deduced amino acid sequences as well as structural and phylogenetic analyses of these genes confirmed the high similarity among actins from ticks in comparison to other species.
da Silva Vaz
Itabajara
I
Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Sapporo 060-0818, Hokkaido, Japan. ita@dna.cbiot.ufrgs.br
Imamura
Saiki
S
Nakajima
Chie
C
de Cardoso
Felipe Cardoso
FC
Ferreira
Carlos Alexandre Sanchez
CA
Renard
Gaby
G
Masuda
Aoi
A
Ohashi
Kazuhiko
K
Onuma
Misao
M
D000199:Actins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics; D005260:Female; D026863:Ixodidae / Q000145:classification / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000199:Actins; D018076:DNA, Complementary