[Cloning and sequencing of Sry gene of muntiacus].
Using the primers from SRY gene--HMG Box for PCR amplification in genomic DNA of Muntiacus reevesi cell strains, a 220bp fragment was obtained in the male but not in the female. The 220bp fragment was cloned into the pGEM-T vector using T/A clone method. The identified positive clone was sequenced. The result shows that 82.6% nucleotides(152bp/184bp) are homologous between Muntiacus Sry and human SRY gene. It suggests that SRY is highly conserved during evolution.
Lu
Xiao-Xuan
XX
Genetic Research Center, Medical College of Southeast University, Nanjing 210009, China. luxiaoxuan@hotmail.com
Zhang
Yue
Y
Shan
Xiang-Nian
XN