IgG, IgA and IgM by formylated rocket immunoelectrophoresis.
Formylated rocket electrophoresis has been investigated as a means of measuring the serum immunoglobulins IgG, IgA, and IgM. This procedure is simpler to carry out than carbamylation. Comparison of formylated rocket results with those from the automated immunoprecipitin (A.I.P.) system and single radial immunodiffusion (S.R.D.) gives correlation coefficients of 0.92 to 0.98 and reproducibility comparable with the AI.P. system and better than S.R.D. The procedure is recommended for use in laboratories when the number of requests for immunoglobulins does not warrant a specific protein analyser.
Slater
L
L
Department of Chemical Pathology, Westminster Medical School, London SW1.
D005557:Formaldehyde / Q000737:chemistry*; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis / Q000379:methods*; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D011233:Precipitin Tests; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005557:Formaldehyde