title: Evaluation of cross-reactivity between Holoptelea integrifolia and Parietaria judaica.
abstract: BACKGROUND
  Holoptelea integrifolia and Parietaria judaica belong to the family Urticaceae, but are geographically distantly located. H. integrifolia is an important pollen allergen of India and sensitizes almost 10% of the atopic population in Delhi. P. judaica, on the other hand, is a very dominant pollen allergen of the Mediterranean region, sensitizing almost 80% of the allergic population. Since both these important pollen allergens belong to the family Urticaceae, the objective of the present study was to assess cross-reactivity between these two pollen allergens from different geographical regions.
  
  
  METHODS
  Cross-reactivity between these two pollen allergens was assessed on the basis of skin prick tests and ELISA, ELISA inhibition and immunoblot inhibition studies.
  
  
  RESULTS
  Out of 44 atopic Indian patients skin prick tested with H. integrifolia extract, 34% were found to be sensitized. All the patients sensitized to H. integrifolia also showed varying degrees of skin positivity to P. judaica pollen extract. ELISA and ELISA inhibition studies suggested strong cross-reactivity between H. integrifolia and P. judaica pollen. Immunoblot inhibition studies revealed that 14-, 16-, 28-, 38-, 42- and 46-kDa proteins are the cross-reactive proteins in H. integrifolia and P. judaica. However, Par j 1, the major allergen of P. judaica, is absent in H. integrifolia pollen.
  
  
  CONCLUSIONS
  H. integrifolia and P. judaica pollens share cross-reactive as well as unique epitopes. The major allergen of P. judaica, Par j 1, seems to be absent in H. integrifolia pollen allergen.
authors.affiliation: Institute of Genomics and Integrative Biology, Delhi University, Delhi, India.
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: R C
authors.initials: RC
authors.lastname: Panzani
authors.forename: S N
authors.initials: SN
authors.lastname: Gaur
authors.forename: R
authors.initials: R
authors.lastname: Ariano
authors.forename: A B
authors.initials: AB
authors.lastname: Singh
mesh_terms: D000485:Allergens / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D006801:Humans; D007194:India; D007558:Italy; D032403:Parietaria / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D027003:Urticaceae / Q000276:immunology*
chemical_list: D000485:Allergens; C105336:PMAI protein, Parietaria judaica; D010940:Plant Proteins