In vitro effect of silicone oil and liquid perfluorocarbons on platelet aggregation.
OBJECTIVE
To study the in vitro effect of silicone oil of different viscosities and liquid perfluorocarbons on platelet aggregation.


METHODS
Silicone oil with a viscosity of 5700 cs and 1000 cs and liquid perfluorocarbons of perfluoroperhydrophenantren and perfluorodecaline were studied to investigate the effect of these agents on platelet aggregation under in vitro conditions. The experiments were performed by using platelet rich plasma with an aggregometer. Aggregation was induced with three different agonists, including 5"-adenosine diphosphate (ADP), epinephrine (EPI), and collagen (Col). The results were obtained as a percentage of maximal aggregation and compared with controls using Kruskal-Wallis one way analysis of variance test.


RESULTS
The tests with ADP as aggregating agent revealed that the percentage of maximal aggregation was a mean of 72.66+/-3.51% for ADP only, 58.66+/-3.05% for silicone oil of 1000 cs, 62.66+/-2.08% for silicone oil of 5700 cs, 56.00+/-7.00% for perfluoroperhydrophenantren, and 52.3+/-3.1 % for perfluorodecaline. With EPI, aggregation was induced in all control samples with a mean of 76+/-9.54%. The mean percentage of maximal aggregation was 66.7+/-3.06 for silicone oil of 1000 cs, 72.33+/-5.5% for silicone oil of 5700 cs, 71.67+/-3.79% for perfluoroperhydrophenantren, and 70.33+/-2.52% for perfluorodecaline. With collagen, it was 86.67+/-1.53% for controls, 83.67+/-3.51% for silicone oil of 1000 cs, 85.33+/-4.51% for silicone oil of 5700 cs, 83.33+/-4.93% for perfluoroperhydrophenantren, and 81.33+/-4.16% for perfluorodecaline. Statistical analysis revealed no significant change in the percentage of maximal aggregation for all tested substances in the experiments.


CONCLUSIONS
Silicone oil of different viscosities and perfluoroperhydrophenantren and perfluorodecaline have minimal antiaggregating effect on platelets. The level of effect is not statistically significant.
Baslar
Z
Z
Department of Internal Medicine Istanbul University, Istanbul, Turkey.
Aras
C
C
Unal
M
M
Kayiran
A
A
Ustundag
S
S
Ozkan
S
S
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005466:Fluorocarbons / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D012827:Silicone Oils / Q000494:pharmacology*; D014783:Viscosity
D005466:Fluorocarbons; D012827:Silicone Oils; C008805:perfluorodecalin; D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine; C047722:perfluorophenanthrene