title: [The factors affecting the efficiency of mutiplex PCR].
abstract: In the research,the outputs of different cycle parameters, PCR buffer and reaction volumes are compared. The results indicated that the annealing temperature, annealing time, elongated time and ingredient of PCR buffer affected mutiplex PCR, but the reaction volume and cycle number had few effect on it.
authors.affiliation: National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
authors.forename: Yin-Hua
authors.initials: YH
authors.lastname: Huang
authors.forename: Xiao-Xiang
authors.initials: XX
authors.lastname: Hu
authors.forename: Wei-Zhuo
authors.initials: WZ
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Feng
authors.forename: Han
authors.initials: H
authors.lastname: Sun
authors.forename: Ning
authors.initials: N
authors.lastname: Li