title: [High expression of human thrombopoietin mutant fusion protein in E. coli].
abstract: OBJECTIVE
  To obtain human thrombopoietin (Tpo)mutant cDNA encoding mature peptide N terminal 1 approximately 196 amino acids and investigate its expression in E. coli JM109.
  
  
  METHODS
  Polymerase chain reaction and DNA recombination techniques were employed. PCR product was inserted into pUC19 vector and sequenced and then cloned into expression vector pMAL-c2.
  
  
  RESULTS
  E. coli JM109 cells with plasmid pMAL -MBP/TpoM were induced by IPTG for 4-5 hours. SDS-PAGE analysis showed that MBP/TpoM molecular weight is about 63KD. Scanning analysis indicated that expressed protein accounts up to 37% of total E. coli proteins.
  
  
  CONCLUSIONS
  The Tpo mutant of interest is successfully expressed in E. coli JM109 cells.
authors.affiliation: Norman Bethune University of Medical Sciences, Changchun 130021.
authors.forename: S
authors.initials: S
authors.lastname: Tian
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Feng
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D013926:Thrombopoietin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D013926:Thrombopoietin