title: [Cloning and permanent expression in eukaryotic cells of rhTpo].
abstract: OBJECTIVE
  To obtain recombinant human thrombopoietin (rhTpo).
  
  
  METHODS
  rhTpo gene was cloned from fetal liver mRNA by means of RT-PCR and subcloned into permanent expressing plasmid RC/CMV in order to form a recombinant permanent expressing plasmid RC/CMV/Tpo.
  
  
  CONCLUSIONS
  After being transferred into CHO cells, the eukaryotic permanent expression of rhTpo, which can enhance murine megakaryocyte colony growth in vitro, was obtained.
authors.affiliation: Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing.
authors.forename: H
authors.initials: H
authors.lastname: Cao
authors.forename: Z
authors.initials: Z
authors.lastname: Ge
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Ding
mesh_terms: D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013926:Thrombopoietin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D013926:Thrombopoietin