Identification of a human B-cell/myeloid common progenitor by the absence of CXCR4.
CXCR4 is a chemokine receptor required for hematopoietic stem cell engraftment and B-cell development. This study found that a small fraction of primitive CD34(+)/CD19(+) B-cell progenitors do not express CXCR4. These CD34(+)/CD19(+)/CXCR4(-) cells were also remarkable for the relative lack of primitive myeloid or lymphoid surface markers. When placed in B-lymphocyte culture conditions these cells matured to express CXCR4 and other surface antigens characteristic of B cells. Surprisingly, when placed in a myeloid culture environment, the CXCR4(-) B-cell progenitors could differentiate into granulocyte, macrophage, and erythroid cells at a high frequency. These data define a novel B-cell/myeloid common progenitor (termed the BMP) and imply a less restrictive pathway of myeloid versus lymphoid development than previously postulated.
Hou
Yong-Hao
YH
Cancer Research and Treatment Center, University of New Mexico Health Science Center, 900 Camino de Salud, Albuquerque, NM 87131, USA.
Srour
Edward F
EF
Ramsey
Heather
H
Dahl
Richard
R
Broxmeyer
Hal E
HE
Hromas
Robert
R
D018941:Antigens, CD19; D018952:Antigens, CD34; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D019070:Cell Lineage; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D022423:Myeloid Cells / Q000737:chemistry / Q000166:cytology*; D019718:Receptors, CXCR4 / Q000032:analysis*
D018941:Antigens, CD19; D018952:Antigens, CD34; D019718:Receptors, CXCR4