[Conserved region analysis of aac(3)-II gene from E. coli aminoglycoside resistance strain].
According to standard K B method, bacteriostatic tests were performed to screen out aminoglycoside resistance bacteria from 47 strains of isolated E.coli. To analyze correlations between the degree of E.coli aminoglycoside resistance and aac(3)-II gene conserved region, PCR amplified aac(3)-II gene conserved regions and were analyzed by DNA sequencing. The results showed that there were two species of aac(3)-II gene type including 65G and 84T or 65A and 84C in the samples. Strains with high activity of modifying enzyme to gentamicin all were 65G and 84T aac(3)-II gene type.
Chen
Jie-Gen
JG
School of Clinical Laboratory Medicine and Public Health, Institute of Cellular and Molecular Medicine, Wenzhou Medical College, Wenzhou 325 027, China.
Lü
Jian-Xin
JX
Lou
Yong-Liang
YL
Peng
Ying
Y
Chen
Xiu-Shu
XS
Lu
Yong-Sui
YS
D000123:Acetyltransferases / Q000235:genetics*; D000583:Amikacin / Q000494:pharmacology; D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000494:pharmacology*; D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics*; D004247:DNA / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005839:Gentamicins / Q000494:pharmacology; D017354:Point Mutation; D014031:Tobramycin / Q000494:pharmacology
D000617:Aminoglycosides; D005839:Gentamicins; D000583:Amikacin; D004247:DNA; D000123:Acetyltransferases; C045539:aminoglycoside N(3')-acetyltransferase; D014031:Tobramycin