Synthetic surfaces for ribonuclease adsorption.
Intact RNA and DNA are of central importance to biochemical research and biotechnology. The preservation of these nucleic acids requires the absence of nuclease activity. Here, radical-mediated polymerization of vinylsulfonate on resin and glass surfaces is shown to produce a high-density poly(vinylsulfonate) coating that sequesters ribonucleases from aqueous solutions quickly and completely. The adsorptive efficacy of this coating exceeds that of other known coatings by > or =10(7)-fold. Surfaces coated with poly(vinylsulfonate) could be used to maintain the integrity of ribonucleic acids in a variety of contexts.
Smith
Bryan D
BD
Department of Biochemistry and Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Soellner
Matthew B
MB
Raines
Ronald T
RT
D000327:Adsorption; D006801:Humans; D012260:Ribonucleases / Q000737:chemistry*
D012260:Ribonucleases