Effects of protein and peptide addition on lipid oxidation in powder model system.
The effect of protein and peptide addition on the oxidation of eicosapentaenoic acid ethyl ester (EPE) encapsulated by maltodextrin (MD) was investigated. The encapsulated lipid (powder lipid) was prepared in two steps, i.e., mixing of EPE with MD solutions (+/- protein and peptides) to produce emulsions and freeze-drying of the resultant emulsions. EPE oxidation in MD powder progressed more rapidly in the humid state [relative humidity (RH) = 70%] than in the dry state (RH = 10%). The addition of soy protein, soy peptide, and gelatin peptides improved the oxidation stability of EPE encapsulated by MD, and the inhibition of lipid oxidation by the protein and the peptides was more dramatic in the humid state. Especially, the oxidation of EPE was almost perfectly suppressed when the lipid was encapsulated with MD + soy peptide during storage in the humid state for 7 days. Several physical properties such as the lipid particle size of the emulsions, the fraction of nonencapsulated lipids, scanning electron microscopy images of powder lipids, and the mobility of the MD matrix were investigated to find the modification of encapsulation behavior by the addition of the protein and peptides, but no significant change was observed. On the other hand, the protein and peptides exhibited a strong radical scavenging activity in the powder systems as well as in the solution systems. These results suggest that a chemical mechanism such as radical scavenging ability plays an important role in the suppression of EPE oxidation in MD powder by soy proteins, soy peptides, and gelatin peptides.
Park
Eun Young
EY
Laboratory of Quality Analysis and Assessment, Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Murakami
Hiroshi
H
Mori
Tomohiko
T
Matsumura
Yasuki
Y
D002214:Capsules; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology; D011134:Polysaccharides; D011208:Powders; D030262:Soybean Proteins / Q000494:pharmacology*
D002214:Capsules; D016166:Free Radical Scavengers; D010455:Peptides; D011134:Polysaccharides; D011208:Powders; D030262:Soybean Proteins; C035276:eicosapentaenoic acid ethyl ester; C008315:maltodextrin; D005780:Gelatin; D015118:Eicosapentaenoic Acid