Bcl-2 and Bax protein expression in human optic nerve axons in the eyeballs after severe trauma and in the eyes with absolute glaucoma.
The aim of our study was to evaluate the immunohistochemical expression of Bcl-2 and Bax protein in optic nerve axons after severe eyeball injury and in the eyes with absolute glaucoma. A series of 19 eyeballs, enucleated because of absolute glaucoma and 41 eyeballs, enucleated, following extensive injury, at the Department of Ophthalmology of the Medical University of Bialystok, were taken into our study. The immunohistochemical reaction was performed with Bcl-2 and Bax protein antibodies and by the LSAB technique. DAB was used in order to visualise the reaction. The optic nerve axons in glaucomatous eyeballs showed statistically significant higher Bax protein expressions than those of Bcl-2 proteins. In the optic nerve axons after severe eyeball injury, a non-significantly higher Bcl-2 protein expression was observed.
Zalewska
R
R
Department of Ophthalmology, Medical University of Bialystok, Poland. renata-zalewska@wp.pl
Reszeć
J
J
Mariak
Z
Z
Sulkowski
S
S
Proniewska-Skretek
E
E
D001369:Axons / Q000473:pathology*; D015353:Eye Enucleation; D005131:Eye Injuries / Q000473:pathology* / Q000601:surgery; D005901:Glaucoma / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D009900:Optic Nerve / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012189:Retrospective Studies; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein