title: Genome-wide survey of non-essential genes required for slowed DNA synthesis-induced filamentous growth in yeast.
abstract: We recently discovered that slowed DNA synthesis induces filamentous differentiation in S. cerevisiae. We screened the BY yeast deletion strains and identified four classes of non-essential genes that are required for both slowed DNA-induced filamentous growth and classic forms of filamentous growth: (a) genes encoding regulators of the actin cytoskeleton and cell polarity, ABP1, CAP2 and HUF1 (=YOR300W), in addition to the previously known BNI1, BUD2, PEA2, SPA2 and TPM1; (b) genes that are likely involved in cell wall biosynthesis, ECM25, GAS1 and PRS3; (c) genes encoding possible regulators of protein secretion, SEC66, RPL21A and RPL34B; (d) genes encoding factors for normal mitochondrial function, IML1 and UGO1. These results showed that pseudohyphal formation involves not the only previously known regulation of the actin cytoskeleton/cell polarity but also regulation of cell wall synthesis, protein secretion and mitochondrial function. Identification of multiple classes of genes that are required for both slowed DNA synthesis-induced and classic forms of filamentous growth confirms that slowed DNA synthesis-induced filamentous growth is bone fide filamentous differentiation.
authors.affiliation: Department of Medical Biochemistry & Genetics, Texas A&M University System Health Science Center, 428 Reynolds Medical Building, College Station, TX 77843-1114, USA.
authors.forename: Christopher Minkyu
authors.initials: CM
authors.lastname: Kang
authors.forename: Yi Wei
authors.initials: YW
authors.lastname: Jiang
mesh_terms: D000199:Actins / Q000235:genetics / Q000502:physiology; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology; D017358:Cell Wall Skeleton / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D003599:Cytoskeleton / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D016681:Genome, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D008928:Mitochondria / Q000235:genetics / Q000502:physiology; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology; D016254:Mutagenesis, Insertional; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development*
chemical_list: D000199:Actins; D017358:Cell Wall Skeleton; D004271:DNA, Fungal; D006918:Hydroxyurea