title: Production of minichaperone (sht GroEL191-345) and its function in the refolding of recombinant human interferon gamma.
abstract: The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg/L broth. In the refolding of recombinant human interferon gamma (rhuIFN-gamma) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.
authors.affiliation: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Guan
authors.forename: Zheng-Zheng
authors.initials: ZZ
authors.lastname: Fei
authors.forename: Man
authors.initials: M
authors.lastname: Luo
authors.forename: Shan-Jing
authors.initials: SJ
authors.lastname: Yao
authors.forename: Man-Gi
authors.initials: MG
authors.lastname: Cho
mesh_terms: D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry* / Q000378:metabolism; D017510:Protein Folding; D011994:Recombinant Proteins
chemical_list: D018834:Chaperonin 60; D011994:Recombinant Proteins; D007371:Interferon-gamma