title: Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes.
abstract: Dehalococcoides ethenogenes is the only bacterium known to reductively dechlorinate the groundwater pollutants, tetrachloroethene (PCE) and trichloroethene, to ethene. Its 1,469,720-base pair chromosome contains large dynamic duplicated regions and integrated elements. Genes encoding 17 putative reductive dehalogenases, nearly all of which were adjacent to genes for transcription regulators, and five hydrogenase complexes were identified. These findings, plus a limited repertoire of other metabolic modes, indicate that D. ethenogenes is highly evolved to utilize halogenated organic compounds and H2. Diversification of reductive dehalogenase functions appears to have been mediated by recent genetic exchange and amplification. Genome analysis provides insights into the organism's complex nutrient requirements and suggests that an ancestor was a nitrogen-fixing autotroph.
authors.affiliation: Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. rekha@tigr.org
authors.forename: Rekha
authors.initials: R
authors.lastname: Seshadri
authors.forename: Lorenz
authors.initials: L
authors.lastname: Adrian
authors.forename: Derrick E
authors.initials: DE
authors.lastname: Fouts
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Eisen
authors.forename: Adam M
authors.initials: AM
authors.lastname: Phillippy
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Methe
authors.forename: Naomi L
authors.initials: NL
authors.lastname: Ward
authors.forename: William C
authors.initials: WC
authors.lastname: Nelson
authors.forename: Robert T
authors.initials: RT
authors.lastname: Deboy
authors.forename: Hoda M
authors.initials: HM
authors.lastname: Khouri
authors.forename: James F
authors.initials: JF
authors.lastname: Kolonay
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Dodson
authors.forename: Sean C
authors.initials: SC
authors.lastname: Daugherty
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Brinkac
authors.forename: Steven A
authors.initials: SA
authors.lastname: Sullivan
authors.forename: Ramana
authors.initials: R
authors.lastname: Madupu
authors.forename: Karen E
authors.initials: KE
authors.lastname: Nelson
authors.forename: Katherine H
authors.initials: KH
authors.lastname: Kang
authors.forename: Marjorie
authors.initials: M
authors.lastname: Impraim
authors.forename: Kevin
authors.initials: K
authors.lastname: Tran
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Robinson
authors.forename: Heather A
authors.initials: HA
authors.lastname: Forberger
authors.forename: Claire M
authors.initials: CM
authors.lastname: Fraser
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Zinder
authors.forename: John F
authors.initials: JF
authors.lastname: Heidelberg
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis; D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000235:genetics* / Q000378:metabolism*; D020440:Gene Duplication; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006859:Hydrogen / Q000378:metabolism; D008969:Molecular Sequence Data; D009591:Nitrogenase / Q000235:genetics / Q000378:metabolism; D009876:Operon; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011809:Quinones / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013750:Tetrachloroethylene / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D000596:Amino Acids; D011809:Quinones; D014157:Transcription Factors; D014874:Water Pollutants, Chemical; D006859:Hydrogen; D010088:Oxidoreductases; C112990:tetrachloroethene dehalogenase; D009591:Nitrogenase; D013750:Tetrachloroethylene