Comparative effect of cyanamide and sodium nitroprusside on indolamine and lipid peroxidation levels in rat brain.
The aim of this study was to compare the effect of cyanamide (CNM) and sodium nitroprusside (SNP) on nitrite (NO2-) and nitrate (NO3-), stable metabolites of nitric oxide (NO), serotonin (5-HT), tryptophan (Trp), and lipid peroxidation (TBARS) levels in rat brain. Twenty-four male Wistar rats (350 g) were used, divided in 3 groups of 8 animals each. Control group I received 0.9 % NaCl, CNM (40 mg/kg) was administered to group II, and SNP (20 microg/kg) to group III; all animals received a single intraperitoneal dose. The animals were sacrificed by decapitation and the brain was homogenized to measure the NO2-, NO3-, 5-HT, and Trp levels by liquid chromatography, and TBARS levels by spectrophotometry. NO2- levels significantly increased (p< 0.01) in the CNM group, while 5-HT and TBARS significantly increased (p = 0.001) both in SNP and CNM groups in relation to the control group. Trp levels presented a slight increase in the CNM group with respect to the control group. The results suggest that free radicals (Nitroxyl and NO) generated by CNM and SNP, respectively, are responsible for oxidative stress induced in brain.
Guzmán
David Calderón
DC
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría.
Vázquez
Ivonne Espitia
IE
Mejia
Gerardo Barragán
GB
Garcia
Ernestina Hernández
EH
Gertrudis
Bernardino Huerta
BH
Urrutia
Elvia Coballase
EC
del Angel
Daniel Santamaría
DS
Olguín
Hugo Juárez
HJ
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D003484:Cyanamide / Q000494:pharmacology*; D007202:Indicators and Reagents; D007211:Indoles / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001679:Biogenic Amines; D007202:Indicators and Reagents; D007211:Indoles; D009566:Nitrates; D017392:Thiobarbituric Acid Reactive Substances; D009599:Nitroprusside; D009569:Nitric Oxide; D012701:Serotonin; D003484:Cyanamide