title: [Immunogenicity analysis of HIV vaccine based on replicating vaccinia virus Tiantan vector].
abstract: OBJECTIVE
  To investigate the immunogenicity of HIV vaccine vTKgpe based on Vaccinia Virus Tiantan vector in mice and rabbits.
  
  
  METHODS
  Mice were inoculated with HIV vaccine vTKgpe by intramuscular (i.m.), intradermal (i.d.) or subcutaneous (s.c.) injections. Blood sample were collected every week, and then antibodies against HIV and vaccinia virus vector were detected. At week 4, some mice were killed and cellular immune responses were examined by flow cytometer. Additionally two rabbits were vaccinated subcutaneously, blood sample were tested as done with mice.
  
  
  RESULTS
  In mice i.m. and s.c. groups, HIV specific antibodies emerged at week 2 and declined at week 4. Antibodies against vector elevated rapidly at week 4, and potentially affected HIV specific antibody detection. Cellular immune responses were only detected in s.c. group. Serum of rabbit showed that anti-HIV antibody appeared at week 2 and maintained for several weeks.
  
  
  CONCLUSIONS
  Vaccine vTKgpe innoculated by i.m. and s.c routes inclined to induce humoral immune responses in mice, but in i.d. group, inclined to induce cellular immune responses. Response to the recombinant vaccinia virus was more sensitive in rabbit than in mice.
authors.affiliation: National Center for AIDS/STD Prevention and Control, China CDC, Beijing 100050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Dan-li
authors.initials: DL
authors.lastname: Duan
authors.forename: Hong
authors.initials: H
authors.lastname: Peng
authors.forename: Hai-li
authors.initials: HL
authors.lastname: Tang
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Jian-yuan
authors.initials: JY
authors.lastname: Liu
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Shao
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D005260:Female; D005822:Genetic Vectors / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood*; D007114:Immunization; D007371:Interferon-gamma / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011995:Recombination, Genetic; D013045:Species Specificity; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D014614:Vaccines, Synthetic; D007371:Interferon-gamma