Use of glnQ as a counterselectable marker for creation of allelic exchange mutations in group B streptococci.
Efficient allelic exchange mutagenesis in group B streptococci (GBS) has been hampered by the lack of a counterselectable marker system. Growth inhibition of GBS by the glutamine analog gamma-glutamyl hydrazide requires glnQ. We have used this phenomenon to create a counterselectable marker system for efficient selection of allelic exchange mutants in GBS.
Tamura
Glen S
GS
Division of Infectious Disease and Department of Pediatrics, Children's Hospital and Regional Medical Center and University of Washington, 4800 Sand Point Way N.E., Seattle, WA 98105, USA. gtamura@u.washington.edu
Bratt
Debra S
DS
Yim
Harry H
HH
Nittayajarn
Aphakorn
A
D018829:Adhesins, Bacterial / Q000235:genetics*; D000483:Alleles; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D005819:Genetic Markers; D005821:Genetic Techniques; D005971:Glutamates / Q000378:metabolism; D006801:Humans; D006834:Hydrazines / Q000494:pharmacology; D009154:Mutation; D012641:Selection, Genetic; D013292:Streptococcus agalactiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D014169:Transformation, Bacterial
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D005819:Genetic Markers; D005971:Glutamates; D006834:Hydrazines; C457279:glnQ protein, E coli; D015500:Chloramphenicol O-Acetyltransferase