title: Sensitive and specific detection of Xanthomonas campestris pv campestris by PCR using species-specific primers based on hrpF gene sequences.
abstract: A sensitive and specific assay was developed to detect bacterial black rot of crucifers caused by Xanthomonas campestris pv. campestris (X. c. pv. campestris), in cabbage seed and plant. Primers XCF and XCR from hrpF homologous to nolX, host recognition protein, were used to amplify a 525 bp DNA fragment. PCR technique was applied to detect the pathogen in naturally infected seed and plant of cabbage. The PCR product was only produced from X. c. pv. campestris among 40 isolates of Xanthomonas strains, Escherichia coli (O157:H7), Pectobacterium carotovorum subsp. carotovorum, and other reference bacteria.
authors.affiliation: National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Park
authors.forename: Byoung Moo
authors.initials: BM
authors.lastname: Lee
authors.forename: Jang
authors.initials: J
authors.lastname: Ho-Hahn
authors.forename: Gil Bok
authors.initials: GB
authors.lastname: Lee
authors.forename: Dong Suk
authors.initials: DS
authors.lastname: Park
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D001937:Brassica / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D016973:Pectobacterium carotovorum / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000382:microbiology; D012680:Sensitivity and Specificity; D013045:Species Specificity; D016959:Xanthomonas campestris / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C106304:HrpF protein, Xanthomonas campestris