Surface-enhanced Raman scattering imaging of single living lymphocytes with multivariate evaluation.
This paper is aimed to show the possibility to determine individual organic compounds introduced into single living cells with surface-enhanced Raman spectroscopy (SERS). Surface enhancement was achieved with gold colloids that were allowed to diffuse into lymphocytes. An introduced analyte, rhodamine 6G, could be imaged together with for example nucleotides and amino acids of the cell. Multivariate evaluation of surface-enhanced Raman images proved to be a powerful tool for the separation of spectral information of various intracellular components. The principal component analysis (PCA) enabled identification of spectra containing different chemical information and separation of the spectral contribution of rhodamine 6G from the complex cellular matrix.
Eliasson
Charlotte
C
Department of Chemistry and Bioscience, Chalmers University of Technology, Kemiuägen 10, SE-41296 Göteborg, Sweden.
Lorén
Anders
A
Engelbrektsson
Johan
J
Josefson
Mats
M
Abrahamsson
Jonas
J
Abrahamsson
Katarina
K
D003585:Cell Biology; D017979:Gold Colloid / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure*; D015999:Multivariate Analysis; D015203:Reproducibility of Results; D012235:Rhodamines / Q000494:pharmacology; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*
D017979:Gold Colloid; D012235:Rhodamines; C026188:rhodamine 6G