[Expression of CD45RA and CD45RO antigens and its implication in leukemias].
OBJECTIVE
To investigate the expression of CD45RA and CD45RO antigens in leukemias and its implication in the diagnosis and differential diagnosis of leukemias.


METHODS
The expression of CD45 RA and CD45RO antigens and other markers on the surface of bone marrow or peripheral blood cells was investigated by indirect immunofluorescence in 104 cases of leukemias. Of them, 33 cases were ALL (23 of B-lineage ALL and 10 of T-ALL), 62 AML, and 9 B-CLL.


RESULTS
Four patterns, CD45RA+/RO-, CD45 RA-/RO+, CD45RA+/RO+ and CD45RA-/RO-, were revealed. 87% of B-lineage ALL displayed CD45 RA+/RO-, 70% of T-ALL CD45RA-/RO+, and 78% of B-CLL CD45RA+/RO+, The frequency of the four patterns was 56% for CD45RA+/RO-, 18% for CD45RA-/RO+, 16% for CD45RA+/RO+ and 10% for CD45RA-/RO-, respectively in AML, and had no correlation with AML subtypes.


CONCLUSIONS
CD45 isoform patterns could be served as supplementary tools for the differential diagnosis of B-lineage ALL, T-ALL and B-CLL.
Li
F
F
Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Shen
D
D
Chen
G
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003937:Diagnosis, Differential; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007938:Leukemia / Q000175:diagnosis* / Q000235:genetics*; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D017493:Leukocyte Common Antigens