Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings.
An inexpensive enzyme-linked immunosorbent assay method for human immunodeficiency virus type 1 quantitation, ultrasensitive p24 antigen assay (Up24), was compared with RNA viral load assay (VL). Up24 had 100% sensitivity of detection at a viral load of >/=30,000, with sensitivity of 46.4% at a viral load of <30,000 (232 specimens from 65 seropositive subjects). The assay was highly reproducible, with excellent correlation between duplicates and among three laboratories.
Respess
Richard A
RA
Global AIDS Program, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mail Stop A-12, Atlanta, GA 30333, USA. rrespess@cdc.gov
Cachafeiro
Ada
A
Withum
David
D
Fiscus
Susan A
SA
Newman
Daniel
D
Branson
Bernard
B
Varnier
Oliviero E
OE
Lewis
Kim
K
Dondero
Timothy J
TJ
D000328:Adult; D016655:HIV Core Protein p24 / Q000097:blood*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load
D016655:HIV Core Protein p24; D012367:RNA, Viral