Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants.
Defects in meiotic recombination in many organisms result in arrest because of activation of a meiotic checkpoint(s). The proximal defect that triggers this checkpoint in mammalian germ cells is not understood, but it has been suggested to involve either the presence of DNA damage in the form of unrepaired recombination intermediates or defects in homologous chromosome pairing and synapsis independent of DNA damage per se. To distinguish between these possibilities in the female germ line, we compared mouse oocyte development in a mutant that fails to form the double-strand breaks (DSBs) that initiate meiotic recombination (Spo11-/-) to mutants with defects in processing DSBs when they are formed (Dmc1-/- and Msh5-/-), and we examined the epistasis relationships between these mutations. Absence of DSB formation caused a partial defect in follicle formation, whereas defects in DSB repair caused earlier and more severe meiotic arrest, which could be suppressed by eliminating DSB formation. Therefore, our analysis reveals that there are both DNA-damage-dependent and -independent responses to recombination errors in mammalian oocytes. By using these findings as a paradigm, we also examined oocyte loss in mutants lacking the DNA-damage checkpoint kinase ATM. The absence of ATM caused defects in folliculogenesis that were similar to those in Dmc1 mutants and that could be suppressed by Spo11 mutation, implying that oocyte death in Atm-deficient animals is a response to defective DSB repair.
Di Giacomo
Monica
M
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, New York, NY 10021, USA.
Barchi
Marco
M
Baudat
Frédéric
F
Edelmann
Winfried
W
Keeney
Scott
S
Jasin
Maria
M
D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D004249:DNA Damage / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D004706:Endodeoxyribonucleases; D004843:Epistasis, Genetic; D004950:Esterases / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D005260:Female; D008540:Meiosis; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins; D009865:Oocytes; D006080:Ovarian Follicle; D028044:Phosphate-Binding Proteins; D011506:Proteins / Q000235:genetics / Q000502:physiology; D011995:Recombination, Genetic; D013573:Synaptonemal Complex; D000099257:Meiotic Recombination Protein SPO11
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D004950:Esterases; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D011506:Proteins; D000099257:Meiotic Recombination Protein SPO11; C513032:Dmc1 protein, mouse; C512847:Msh5 protein, mouse