Metaphase and interphase cytogenetics in fibroadenomas of the breast.
Short-term cultures of fifty-two samples of fibroadenomas were cytogenetically analyzed. Thirty-three of the successfully karyotyped fibroadenomas were further investigated for the presence of amplifications in the CCND1, c-MYC and HER/2-neu genes by means of FISH analysis. Compared to carcinomas, fibroadenomas seem to have less complex cytogenetic rearrangements and limited alterations on HER-2/neu, CCND1 and c-MYC loci. A cytogenetic subgroup of fibroadenomas with hyperdiploid karyotypes and only numerical changes was observed. Amplification of CCND1 seems to play a more substantial role in benign tumor progression. These findings confirm that fibroadenomas do have genetic alterations and support the hypothesis that a fibroadenoma subset displays changes also found in carcinomas, thus indicating that patients belonging to this group might have an increased risk for subsequent breast cancer.
Rizou
Helen
H
Department of Genetics, Saint Savas Anticancer Hospital, 171 Alexandras Ave, Athens, Greece. helen_rizou@yahoo.com
Bardi
Georgia
G
Arnaourti
Maria
M
Apostolikas
Nikiforos
N
Sfikas
Kostas
K
Charlaftis
Antonios
A
Polichronis
Athanassios
A
Agnantis
Niki J
NJ
Pandis
Nikos
N
D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D002869:Chromosome Aberrations / Q000145:classification*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D018226:Fibroadenoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007399:Interphase / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D032681:Spectral Karyotyping / Q000379:methods*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm