title: Genes involved in formation and attachment of a two-carbon chain as a component of eurekanate, a branched-chain sugar moiety of avilamycin A.
abstract: Eurekanate belongs to the important class of branched-chain carbohydrates present in a wide variety of natural sources. It is a component of avilamycin A, a potent inhibitor of bacterial protein synthesis targeting the 50S ribosomal subunit. The present work provides experimental proof for the function of two genes of the avilamycin biosynthetic gene cluster, aviB1 and aviO2, that are both involved in avilamycin structure modification. The functions of both genes were identified by gene inactivation experiments and nuclear magnetic resonance analyses of extracts produced by the mutants. We suggest that both AviO2 and AviB1 are involved in the biosynthesis of eurekanate within avilamycin biosynthesis. Moreover, two other genes (aviO1 and aviO3) have been inactivated, resulting in a breakdown of avilamycin production in the mutants ITO1 and ITO3, which clearly shows the essential role of both enzymes in avilamycin biosynthesis. The exact functions of both aviO1 and aviO3 remained unknown.
authors.affiliation: Institut für Pharmazeutische Wissenschaften, Pharmazeutische Biologie und Biotechnologie, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Treede
authors.forename: Gerd
authors.initials: G
authors.lastname: Hauser
authors.forename: Agnes
authors.initials: A
authors.lastname: Mühlenweg
authors.forename: Carsten
authors.initials: C
authors.lastname: Hofmann
authors.forename: Maraike
authors.initials: M
authors.lastname: Schmidt
authors.forename: Gabriele
authors.initials: G
authors.lastname: Weitnauer
authors.forename: Steffen
authors.initials: S
authors.lastname: Glaser
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005816:Genetic Complementation Test; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D005810:Multigene Family; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; C006653:avilamycin; D002244:Carbon