Heart transplantation in baboons using alpha1,3-galactosyltransferase gene-knockout pigs as donors: initial experience.
Hearts from alpha1,3-galactosyltransferase knockout pigs (GalT-KO, n = 8) were transplanted heterotopically into baboons using an anti-CD154 monoclonal antibody-based regimen. The elimination of the galactose-alpha1,3-galactose epitope prevented hyperacute rejection and extended survival of pig hearts in baboons for 2-6 months (median, 78 d); the predominant lesion associated with graft failure was a thrombotic microangiopathy, with resulting ischemic injury. There were no infectious complications directly related to the immunosuppressive regimen. The transplantation of hearts from GalT-KO pigs increased graft survival over previous studies.
Kuwaki
Kenji
K
Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, MGH-East, 13th Street, Boston, Massachusetts 02129, USA.
Tseng
Yau-Lin
YL
Dor
Frank J M F
FJ
Shimizu
Akira
A
Houser
Stuart L
SL
Sanderson
Todd M
TM
Lancos
Courtney J
CJ
Prabharasuth
Derek D
DD
Cheng
Jane
J
Moran
Kathleen
K
Hisashi
Yosuke
Y
Mueller
Nicolas
N
Yamada
Kazuhiko
K
Greenstein
Julia L
JL
Hawley
Robert J
RJ
Patience
Clive
C
Awwad
Michel
M
Fishman
Jay A
JA
Robson
Simon C
SC
Schuurman
Henk-Jan
HJ
Sachs
David H
DH
Cooper
David K C
DK
D000818:Animals; D030801:Animals, Genetically Modified; D004187:Disaccharides / Q000276:immunology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005700:Galactosyltransferases / Q000235:genetics* / Q000378:metabolism; D016027:Heart Transplantation; D009206:Myocardium / Q000473:pathology; D010215:Papio; D013552:Swine; D014183:Transplantation, Heterologous / Q000276:immunology
D004187:Disaccharides; C055075:galactosyl-(1-3)galactose; D005700:Galactosyltransferases