title: [Angiogenic activity and VEGF level in sera of diabetic patients with non-proliferative retinopathy].
abstract: Diabetic retinopathy is one of the most common causes of blindness. The majority of studies concerning this problem were performed in patients with late stage of disease, demonstrating proliferative retinopathy, when malformations of retinal vessels became irreversible. Numerous angiogenic factors related to retinal angiogenesis have been described. Among them, VEGF is thought to be the major mediator of proliferative retinopathy. Our present study was performed on sera collected from patients with earlier stage of ocular complications, manifested as background retinopathy. We have believed that the knowledge of the earliest events leading to pathological angiogenesis may be valuable for establishing some prophylactic regimens. Sera of 22 persons with DM1, aged 33-70 years, 37 persons with DM2, aged 37-79 years, 51 healthy people, aged 22-80 years (as controls) were studied. Direct serum-induced cutaneous angiogenesis test in mice (SIA) was applied. VEGF concentration in sera was mesured by ELISA (R & D Systems) test. Angiogenic activity and VEGF concentration of DM1 patients sera were highly statistically lower than these parameters in DM2 patients and controls.
authors.affiliation: Katedry i Kliniki Okulistyki II Wydziału Lekarskiego Akademii Medycznej w Warszawie.
authors.forename: Piotr
authors.initials: P
authors.lastname: Skopiński
authors.forename: Barbara
authors.initials: B
authors.lastname: Duda-Król
authors.forename: Anna
authors.initials: A
authors.lastname: Lipińska
authors.forename: Ewa
authors.initials: E
authors.lastname: Sommer
authors.forename: Joanna
authors.initials: J
authors.lastname: Chorostowska-Wynimko
authors.forename: Urszula
authors.initials: U
authors.lastname: Demkow
authors.forename: Jerzy
authors.initials: J
authors.lastname: Szaflik
authors.forename: Ewa
authors.initials: E
authors.lastname: Skopińska-Rózewska
authors.forename: Ewa
authors.initials: E
authors.lastname: Struzik
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000503:physiopathology; D003930:Diabetic Retinopathy / Q000097:blood* / Q000150:complications*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D012016:Reference Values; D015861:Retinal Neovascularization / Q000097:blood* / Q000150:complications*; D012867:Skin / Q000098:blood supply; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D042461:Vascular Endothelial Growth Factor A