[Application of DNA extraction and analysis of its influence factors].
To explore influence factors of DNA extraction, the protease and phenol-chloroform method was used to extract DNA in whole blood, fresh tissues, frozen tissues embedding with OCT and tissues embedding in paraffin. It results that 200-300 microg DNA was extracted from 1 ml whole blood or 0.2 g fresh tissues or frozen tissue embedding with OCT. DNA extracted from paraffin specimen can be directly used in PCR amplification. Purity DNA can be extracted from four kinds of different tissues. OCT hasn't harmful effect on tissue DNA extraction.
Dong
Ming
M
Cancer Institute of First Hospital, China Medical University, Shenyang 110001, China. dongm_97@yahoo.com
Gong
Yue-Hua
YH
Wang
Lan
L
Yuan
Yuan
Y