title: H9c2 cardiac muscle cells express all somatostatin receptor subtypes.
abstract: The aim of the present study was to verify the hypothesis that SS receptor subtypes (SSTRs) are expressed by H9c2 cardiac muscle cells. SSTRs expression was investigated by RT-PCR and Western blot analysis at both mRNA and protein level. Our findings demonstrate that H9c2 cells express all SSTR subtypes I-5 (SSTRI-5) at the mRNA and protein level. Thus, H9c2 cells would represent a new model to study the direct biological activities of SS and its analogues at the cardiac level.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Turin, Turin, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Granata
authors.forename: L
authors.initials: L
authors.lastname: Trovato
authors.forename: S
authors.initials: S
authors.lastname: Destefanis
authors.forename: F
authors.initials: F
authors.lastname: Settanni
authors.forename: E
authors.initials: E
authors.lastname: Ghigo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique; D032383:Myocytes, Cardiac / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D017481:Receptors, Somatostatin