The symptom difference induced by tobacco mosaic virus and tomato mosaic virus in tobacco plants containing the N gene is determined by movement protein gene.
Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) are two closely related viruses in the genus Tobamovirus, but they induce obviously different sizes of necrotic lesions in tobacco plants containing the N gene. Comparison of the symptoms produced by TMV, ToMV and a chimaeric virus (T/OMP), in which the TMV movement protein (MP) gene was replaced by the ToMV MP gene, showed T/OMP caused necrotic lesions that were similar in size to those of ToMV in tobacco plants containing the N gene. The coat protein and MP of the three viruses accumulated in planta with similar levels, and the replication level of TMV and T/OMP in protoplasts also had no difference. Comparison of the activities of defense-related enzymes (PAL, POD and PPO) induced by the three viruses also showed that the variability of enzyme activity induced by T/OMP was similar to that induced by TMV, but different from that induced by ToMV. The results indicate that the size difference of necrotic lesions induced by TMV and ToMV in tobacco plants containing the N gene results from the functional difference of their MP genes.
Yu
Cui
C
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
Hu
Dongwei
D
Dong
Jiahong
J
Cui
Xiaofeng
X
Wu
Junjie
J
Yu
Jialin
J
Zhou
Xueping
X
D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D053830:Plant Viral Movement Proteins; D014026:Nicotiana / Q000033:anatomy & histology / Q000235:genetics / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins