[Immunogenicity analysis of HIV vaccine based on replicating vaccinia virus Tiantan vector].
OBJECTIVE
To investigate the immunogenicity of HIV vaccine vTKgpe based on Vaccinia Virus Tiantan vector in mice and rabbits.


METHODS
Mice were inoculated with HIV vaccine vTKgpe by intramuscular (i.m.), intradermal (i.d.) or subcutaneous (s.c.) injections. Blood sample were collected every week, and then antibodies against HIV and vaccinia virus vector were detected. At week 4, some mice were killed and cellular immune responses were examined by flow cytometer. Additionally two rabbits were vaccinated subcutaneously, blood sample were tested as done with mice.


RESULTS
In mice i.m. and s.c. groups, HIV specific antibodies emerged at week 2 and declined at week 4. Antibodies against vector elevated rapidly at week 4, and potentially affected HIV specific antibody detection. Cellular immune responses were only detected in s.c. group. Serum of rabbit showed that anti-HIV antibody appeared at week 2 and maintained for several weeks.


CONCLUSIONS
Vaccine vTKgpe innoculated by i.m. and s.c routes inclined to induce humoral immune responses in mice, but in i.d. group, inclined to induce cellular immune responses. Response to the recombinant vaccinia virus was more sensitive in rabbit than in mice.
Liu
Ying
Y
National Center for AIDS/STD Prevention and Control, China CDC, Beijing 100050, China.
Duan
Dan-li
DL
Peng
Hong
H
Tang
Hai-li
HL
Liu
Sha
S
Zhang
Ning
N
Wang
Ning
N
Liu
Jian-yuan
JY
Shao
Yi-ming
YM
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D005260:Female; D005822:Genetic Vectors / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood*; D007114:Immunization; D007371:Interferon-gamma / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011995:Recombination, Genetic; D013045:Species Specificity; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology
D016915:AIDS Vaccines; D015483:HIV Antibodies; D014614:Vaccines, Synthetic; D007371:Interferon-gamma