The influence of the chemical composition of cell culture material on the growth and antibody production of hybridoma cells.
The multiplication and antibody production of murine hybridoma cells cultured on five different polymer membranes were tested and compared with conventional tissue culture polystyrene (TCPS). Membranes were prepared from polyacrylonitrile (PAN) and acrylonitrile copolymerized with N-vinylpyrrolidone (NVP20, NVP30), Na-methallylsulfonate (NaMAS) and N-(3-amino-propyl-methacrylamide-hydrochloride) (APMA). Cell number and antibody concentration were quantified as criteria for viability and productivity. Adhesion of hybridoma cells was characterized by vital and scanning electron microscopy. The results suggest that a strong adhesion of cells, observed on APMA and TCPS, increased cell growth but reduced monoclonal antibody production. In contrast membranes with lowered adhesivity such as NVP20 provided favourable conditions for monoclonal antibody production. In addition it was shown that this membrane also possessed a minor fouling as indicated by the low decrease of water flux across the membrane after protein adsorption. It was concluded that NVP20 could be a suitable material for the development of hollow fibre membranes for bioreactors.
Heilmann
K
K
Department of Biotechnology, University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Strasse 24-25, Building 25, D-14476 Golm, Germany.
Groth
Th
T
Behrsing
O
O
Albrecht
W
W
Schossig
M
M
Lendlein
A
A
Micheel
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D003470:Culture Media / Q000378:metabolism*; D006825:Hybridomas / Q000166:cytology* / Q000502:physiology*; D007074:Immunoglobulin G / Q000276:immunology; D008567:Membranes, Artificial; D051379:Mice
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007074:Immunoglobulin G; D008567:Membranes, Artificial