Poly rC-oligo dG specific template activity of mouse viral DNA polymerase detected by isoelectric focusing.
An acid fraction from polyacrylamide gel isoelectric focusing contained only DNA polymerase template activity for (rC)n-(dG)12-18 with little or no activity with (rA)n-(dT)12-18. A factor which stimulated (rC)n-(dG) 12-18 activity was also detected in low molecular weight fractions from G-100 Sephadex chromatography of the purified enzyme. Since (rC)n-(dG)12-18 acitivty is thought to be descriptive of viral DNA polymerase, this stimulation is proposed to be significant for the regulation and specificity of viral DNA synthesis.
Ingalls
P G
PG
Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory Berkeley, CA 94720, USA.
Bartholomew
J C
JC
Bennett
E L
EL
D000818:Animals; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D009052:Leukemia Virus, Murine / Q000201:enzymology; D051379:Mice; D041681:NIH 3T3 Cells; D011068:Poly G / Q000378:metabolism*; D006163:Ribonuclease T1 / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D009053:Sarcoma Viruses, Murine / Q000201:enzymology; D013379:Substrate Specificity; D013698:Templates, Genetic
D004279:DNA, Viral; D011068:Poly G; C026628:poly(dG); D004259:DNA-Directed DNA Polymerase; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic