[SSS method for screening cDNA library].
A quick and simple method subsection screening (SSS) method for screening cDNA library by PCR was established. With this method, cDNA phage plate was cut into several blocks and a couple of primers was designed according to target gene. And then the target genes were obtained by screening cDNA library. Comparing with other methods,this method has many advantages such as controlled range and clear target,and also quick and simple for obtaining the target genes. It is possible to get a target gene in one week in general by this method. Thus,the CHI gene, F3'H gene, HSP gene and one HSP partial fragment, were obtained respectively in half month in our lab. We can get twice the result with half the effort when we screen several genes in the same time. It is also suitable to screen other libraries.
Qu
Wen-Quan
WQ
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, No.249101070, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Jin
Zhi-Ping
ZP
Zhao
De-Xiu
DX
Chen
Ya-Qiong
YQ
Li
Ming-Yang
MY