Suppression of GDNF production by MPSS treatment following spinal cord injury in the rat.
The synthetic glucocorticosteroid methylprednisolone sodium succinate (MPSS) has been clinically used for the treatment of acute spinal cord injury (SCI) to promote the recovery of neurological functions. However, the mechanisms of its beneficial actions are not entirely understood. Experimental evidence suggests that MPSS may contribute to some extent to neuroprotection in SCI. On the other hand, glial cell line-derived neurotrophic factor (GDNF) acts as a potent survival factor for several neuronal populations, providing a therapeutic promise for neurological disorders. This experiment demonstrated, for the first time, the time-effect relationship of MPSS on GDNF gene expression and protein synthesis in the injured spinal cord tissues.
Nakashima
Shojiro
S
Department of Orthopaedic Surgery, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya, 466-8550, Japan. shojiro@med.nagoya-u.ac.jp
Matsuyama
Yukihiro
Y
Yu
Yimin
Y
Kiuchi
Kazutoshi
K
Ishiguro
Naoki
N
D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008776:Methylprednisolone Hemisuccinate / Q000627:therapeutic use*; D009414:Nerve Growth Factors / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism; D013997:Time Factors
D039481:CD11b Antigen; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D008776:Methylprednisolone Hemisuccinate