[Pituitary-adrenal function in female mice with mutation Agouti yellow (A(y))].
Agouti protein is a paracrine signaling factor modulating action of ACTH and alpha-MSH. Dominant mutation Ay causes ectopic, ubiquitous expression of Agouti protein in mice. It was shown that Ay mutation increased stress-induced hypothalamo-adrenal activity in male mice. There is a sex difference in the hypothalamo-pituitary-adrenal axis in rodents. The aim of this study was to test effects of ectopic overexpression of Agouti protein on pituitary-adrenal function in female mice. Female mice of C57Bl/6J strain with Ay mutation (Ay/alpha) and with mutation nonagouti (alpha/alpha; lack of Agouti protein) were used. Ay/alpha-females had an increased blood level of corticosterone and ACTH after 10-minute restriction as compared with alpha/alpha-females. The adrenal threshold sensitivity and reaction to exogenous ACTH in vivo suggests that increased corticosterone reaction to emotional stress is caused by increased pituitary stimulation.
Karkaeva
N R
NR
Iakovleva
T V
TV
Makarova
E N
EN
Shevchenko
A Iu
AIu
Bazhan
N M
NM
D000311:Adrenal Glands / Q000187:drug effects / Q000502:physiology; D000324:Adrenocorticotropic Hormone / Q000097:blood; D054366:Agouti Signaling Protein; D000818:Animals; D003345:Corticosterone / Q000097:blood; D005260:Female; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D051379:Mice; D009154:Mutation; D010913:Pituitary-Adrenal System / Q000502:physiology*; D012737:Sex Factors; D013315:Stress, Psychological / Q000503:physiopathology
D054366:Agouti Signaling Protein; D036341:Intercellular Signaling Peptides and Proteins; C515183:a protein, mouse; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone