Effects of lymphocyte isolation and timing of processing on detection of CD127 expression on T cells in human immunodeficiency virus-infected patients.
Decreases in the detection of CD127 expression on T cells of human immunodeficiency virus-infected patients by flow cytometry can occur by delayed processing or by peripheral blood mononuclear cell isolation and cryopreservation. These observations should be considered in the interpretation of functional studies and the planning of multicenter clinical trials.
Higgins
Jeanette
J
AIDS Monitoring Laboratory, Clinical Services Program, Science Applications International Corp., Frederick, Maryland, USA.
Metcalf
Julia A
JA
Stevens
Randy A
RA
Baseler
Michael
M
Nason
Martha C
MC
Lane
H Clifford
HC
Sereti
Irini
I
D000328:Adult; D000368:Aged; D016477:Artifacts; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D020395:Receptors, Interleukin-7 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D020395:Receptors, Interleukin-7