Pestivirus exposure in free-living and captive deer in Austria.
During the hunting season of 2001-02, blood and spleen samples from 59 red deer (Cervus elaphus), 77 roe deer (Capreolus capreolus), four fallow deer (Dama dama), and five chamois (Rupicapra rupicapra) were collected from nine hunting districts (n = 133) and one deer farm (n = 12) in southern Austria. Sera were tested for antibodies against bovine viral diarrhea virus (BVDV) with an enzyme-linked immunosorbent assay (ELISA) and virus neutralization tests against three BVDVs and one border disease virus strain. Reverse transcriptase polymerase chain reaction was used for detection of pestivirus-specific RNA in spleen samples. Antibodies were detected in one serum sample when using ELISA and virus neutralization tests. Results of the virus neutralization tests of this sample provided strong evidence for the exposure to the BVDV-1 genotype. The spleen samples were negative for pestivirus-specific RNA.
Krametter
Reinhild
R
II. Medical Clinic for Ruminants and Swine, Veterinary University of Vienna, 1210 Vienna, Austria. reinhild.krametter@vu-win.ac.at
Nielsen
Søren Saxmose
SS
Loitsch
Angelika
A
Froetscher
Wolfgang
W
Benetka
Viviane
V
Moestl
Karin
K
Baumgartner
Walter
W
D000818:Animals; D000829:Animals, Domestic / Q000821:virology; D000835:Animals, Wild / Q000821:virology; D000914:Antibodies, Viral / Q000097:blood*; D001317:Austria / Q000453:epidemiology; D001882:Border Disease / Q000453:epidemiology; D018083:Border disease virus / Q000276:immunology / Q000302:isolation & purification; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology; D002417:Cattle; D003670:Deer / Q000821:virology*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification; D004781:Environmental Exposure; D005260:Female; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D010576:Pestivirus / Q000276:immunology* / Q000302:isolation & purification*; D018182:Pestivirus Infections / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016036:Seroepidemiologic Studies; D013154:Spleen / Q000821:virology
D000914:Antibodies, Viral