[Strategies for development of SSR molecular markers].
SSR molecular markers have been used widely in many genetic studies and become one of the most popular molecular markers due to their high polymorphism, abundant informativeness, convenience of assay by PCR and distribution throughout the genome. The major drawback of SSR molecular markers is that their primers need to be designed according to the isolated sequence flanking the SSR from species that are being examined for the first time. The aim of the present paper is to review the several representative methods of SSR isolation described in the literature and provide useful information in making appropriate choices among the large number of currently available options.
Zhang
Zeng-Cui
ZC
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210 095, China. zengcui_zhang@hotmail.com
Hou
Xi-Lin
XL
D000818:Animals; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA