title: PCR amplification with primers based on IS2404 and GC-rich repeated sequence reveals polymorphism in Mycobacterium ulcerans.
abstract: We describe a simple genotyping method for Mycobacterium ulcerans based on PCR amplification of genomic regions between IS2404 and a frequently repeated GC-rich sequence. Application of this method to a global collection produced 10 M. ulcerans genotypes corresponding to their geographic origin.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp, Belgium.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ablordey
authors.forename: Roman
authors.initials: R
authors.lastname: Kotlowski
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
mesh_terms: D000349:Africa / Q000453:epidemiology; D001208:Asia / Q000453:epidemiology; D001315:Australia / Q000453:epidemiology; D015373:Bacterial Typing Techniques; D017931:DNA Primers; D004251:DNA Transposable Elements; D020862:GC Rich Sequence; D005838:Genotype; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology* / Q000382:microbiology; D019911:Mycobacterium ulcerans / Q000145:classification* / Q000235:genetics*; D010219:Papua New Guinea / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements