Effect of inoculum size on detection of Candida growth by the BACTEC 9240 automated blood culture system using aerobic and anaerobic media.
Simulated candidemia was produced with 20 Candida isolates at three inoculum sizes (100, 10, and 1 CFU/ml of blood). Growth detection was better with larger inocula. The time to growth detection was shorter with larger inocula. Inoculum size does effect Candida growth detection and time to detection in BACTEC 9240 automated systems.
George
Benjamin J
BJ
Department of Medicine, Brooke Army Medical Center, Fort Sam Houston, TX 78234, USA.
Horvath
Lynn L
LL
Hospenthal
Duane R
DR
D000332:Aerobiosis; D000693:Anaerobiosis; D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification; D002176:Candida albicans / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D003470:Culture Media; D016469:Fungemia / Q000382:microbiology*; D006801:Humans; D008828:Microbiological Techniques; D011933:Reagent Kits, Diagnostic
D003470:Culture Media; D011933:Reagent Kits, Diagnostic