Array biosensor for detection of ochratoxin A in cereals and beverages.
Contamination of food by mycotoxins occurs in minute quantities, and therefore, there is a need for a highly sensitive and selective device that can detect and quantify these organic toxins. We report the development of a rapid and highly sensitive array biosensor for the detection and quantitation of ochratoxin A (OTA). The array biosensor utilizes a competitive immunoassay format. Immobilized OTA derivatives compete with toxin in solution for binding to fluorescent anti-OTA antibody spiked into the sample. This competition is quantified by measuring the formation of the fluorescent immunocomplex on the waveguide surface. The fluorescent signal is inversely proportional to the concentration of OTA in the sample. Analyses for OTA in buffer and a variety of food and beverage samples were performed. Samples were extracted with methanol, without any sample cleanup or preconcentration step prior to analysis. The limit of detection for OTA in several cereals ranged from 3.8 to 100 ng/g, while in coffee and wine, detection limits were 7 and 38 ng/g, respectively.
Ngundi
Miriam M
MM
Center of Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, D.C. 20375, USA.
Shriver-Lake
Lisa C
LC
Moore
Martin H
MH
Lassman
Michael E
ME
Ligler
Frances S
FS
Taitt
Chris R
CR
D001628:Beverages / Q000032:analysis / Q000382:microbiology*; D015374:Biosensing Techniques / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry / Q000382:microbiology*; D007118:Immunoassay; D009793:Ochratoxins / Q000032:analysis*; D012680:Sensitivity and Specificity
D009793:Ochratoxins; C025589:ochratoxin A