title: Clinical isolates of Staphylococcus aureus vary in ability to stimulate cytokine expression in human endothelial cells.
abstract: Human umbilical vein endothelial cells (HUVEC) were infected for 24 h with 18 well-characterized Staphylococcus aureus isolates, and the supernatants from infected HUVEC were analysed for interleukin (IL)-1beta, tumour necrosis factor-alpha, IL-6, IL-8, IL-10, IL-12p70, growth-related oncogene (GRO)-alpha, granulocyte macrophage colony-stimulating factor (GM-CSF) and regulated upon activation, normal T cell expressed and secreted (RANTES) by immunoassay. All staphylococcal isolates induced the expression of IL-6, IL-8, GRO-alpha, GM-CSF and RANTES. The magnitude of cytokine expression varied between isolates. Staphylococcus aureus inducing high expression of one of these cytokines also showed simultaneous high expression of the other four, indicating a common mechanism for the ability of individual S. aureus to induce expression of these cytokines. No direct correlation between cytokine expression and adhesion of S. aureus to HUVEC was observed, indicating that bacterial properties besides adhesion contribute to the activation of HUVEC.
authors.affiliation: Department of Natural Science and Biomedicine, School of Health Sciences, Jönköping, Sweden. jan.strindhall@hhj.hj.se
authors.forename: J
authors.initials: J
authors.lastname: Strindhall
authors.forename: P-E
authors.initials: PE
authors.lastname: Lindgren
authors.forename: S
authors.initials: S
authors.lastname: Löfgren
authors.forename: E
authors.initials: E
authors.lastname: Kihlström
mesh_terms: D001422:Bacterial Adhesion; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000096:biosynthesis; D054360:Chemokine CXCL1; D019743:Chemokines, CXC / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000276:immunology* / Q000382:microbiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D014774:Virulence
chemical_list: C475829:CXCL1 protein, human; D018946:Chemokine CCL5; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D007378:Interleukins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor