Inhibitory effects of polysaccharides isolated from Phellinus gilvus on benzo(a)pyrene-induced forestomach carcinogenesis in mice.
OBJECTIVE
Although polysaccharides from Phellinus mushrooms are a well-known material with anti-tumor properties, there is no information about the effect of polysaccharides from Phellinus gilvus (PG) on tumor. The modulating effect of polysaccharides isolated from PG on the benzo(a)pyrene (BaP)-induced forestomach carcinogenesis in ICR female mice was investigated in this study.


METHODS
A forestomach carcinogenesis model was established in 40 ICR female mice receiving oral administration of BaP for 4 wk. The mice were randomly assigned to 4 groups (10 each). The mice in each group were treated with sterile water or PG for 4 and 8 wk (SW4, PGW4, SW8, and PGW8 groups). Eight or 12 wk after the first dose of BaP, forestomachs were removed for histopathological and RT-PCR analysis.


RESULTS
In histopathological changes and RT-PCR analysis, sterile water-treated mice showed significant hyperplasia of the gastric mucosa with a significantly increased expression of mutant p53 mRNA compared to mice treated with PG for 8 wk.


CONCLUSIONS
Polysaccharides isolated from PG may inhibit BaP-induced forestomach carcinogenesis in mice bydown-regulating mutant p53 expression.
Bae
Jae-Sung
JS
College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Jang
Kwang-Ho
KH
Yim
Hyunee
H
Park
Seung-Chun
SC
Jin
Hee-Kyung
HK
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001564:Benzo(a)pyrene; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006965:Hyperplasia; D051379:Mice; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D000970:Antineoplastic Agents; D011134:Polysaccharides; D016159:Tumor Suppressor Protein p53; D001564:Benzo(a)pyrene