title: [Study on the production condition of extraction in combination hydrolysis in situ for isolating diosgenin].
abstract: OBJECTIVE
  To study the production technology of extraction in combination hydrolysis in situ for isolating diosgenin from Dioscorea nipponica by orthogonal design.
  
  
  METHODS
  The optimum production conditions were investigated with the recovery of diosgenin as an index by extraction in combination hydrolysis in situ, and were compared with the traditional method.
  
  
  RESULTS
  Extraction in combination hydrolysis in situ conducted in 1.5 mol x L(-1) sulfuric acid of water containing 75% isopropanol at 100 degrees C for 4.5 h could get higher recovery of diosgenin than traditional methods.
  
  
  CONCLUSIONS
  This production technology can get higher recovery of diosgenin, and it is simple, time and money saving.
authors.affiliation: School of Biological and Environmental Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China. wangjunjs@tom.com
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Ke-di
authors.initials: KD
authors.lastname: Yang
authors.forename: Liu-qing
authors.initials: LQ
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ouyang
mesh_terms: D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000302:isolation & purification*; D006868:Hydrolysis; D011312:Pressure; D013464:Sulfuric Acids; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D013464:Sulfuric Acids; D004144:Diosgenin; C033158:sulfuric acid