[Progress in ooplasmic transfer].
Many studies indicated mitochondria in oocytoplasm affects obviously the fertilization of the oocytes and early embryo development. The decrease of ATP content and mitochondrial DNA(mtDNA) content, and mtDNA defects can make the fertilization of oocytes and embryo development decreased, which is the key one of reason that the reproductive ability is low in aged women and aged animal. Ooplasmic transfer increases the fertilizability of the aged oocytes and the early embryonic viability, it has led to the birth of health babies, and become a hotspot in the realm of human assisted reproduction biotechnology and animal clone. However, Ooplasmic transfer can lead to mtDNA become heteroplastic, which is the mtDNA of donor and recipient coexist in offspring. At present, how the heterogeneous ooplasm influences the embryogenesis and development is not entirely clear. This paper reviews the ooplasmic transfer in summary, the relation between ooplasm and fertilization and embryo development, the hereditary behavior of heterogeneous mitochondria DNA and it's examination after ooplasmic transfer.
Li
Jun-Feng
JF
Chongqing Key Lab of Forage & Herbivore College of Animal Science and Technology, Southwest Agriculture University, Chongqing 400 716, China. li_jfcn@sina.com
Zhang
Jia-Hua
JH
D000818:Animals; D003593:Cytoplasm / Q000637:transplantation*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D005260:Female; D006801:Humans; D018587:Oocyte Donation; D009865:Oocytes
D004272:DNA, Mitochondrial