Transcriptome profiles of host gene expression in a monkey model of human malaria.
We used human microarrays to examine gene expression in a rhesus monkey model of human Plasmodium vivax malaria (P. cynomolgi in Macaca mulatta). Whole-blood cells were collected for extraction of RNA before infection, during both the initial liver phase of infection and bloodstream infection, and during the course of 2 bloodstream relapses. Clustering analysis showed that similarities in gene expression were greater at similar stages of the protocol for the 2 different monkeys than for the same monkey at different stages of the protocol. Interestingly, a large number of genes involved in RNA processing showed distinct down-regulation during the initial liver phase of infection. When only up-regulated genes were examined, there was evidence of an increasing number of "defense response" genes as the infection evolved but not of "cytoskeleton" genes (P</=.001). These results demonstrate the value of microarrays for studying the response of the primate transcriptome to malaria infection; they suggest that the host response is modulated by groups of genes.
Ylostalo
Joni
J
Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana, USA.
Randall
Amber C
AC
Myers
Tereance A
TA
Metzger
Michael
M
Krogstad
Donald J
DJ
Cogswell
Frank B
FB
D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008253:Macaca mulatta; D008288:Malaria / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016789:Plasmodium cynomolgi / Q000472:pathogenicity*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome; D014158:Transcription, Genetic
D011506:Proteins; D020543:Proteome