title: Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells.
abstract: Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction - but not peptidoglycan - of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU-1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU-1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU-1 cells in a dose- and time-dependent manner. Furthermore, treatment of the cells with 50 microg/ml and 100 microg/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU-1 cell growth is mainly attributable to the induction of apoptosis.
authors.affiliation: Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-741, South Korea.
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002843:Chromatin / Q000187:drug effects; D003593:Cytoplasm / Q000737:chemistry*; D006801:Humans; D013294:Lactococcus lactis; D013274:Stomach Neoplasms
chemical_list: D000970:Antineoplastic Agents; D002457:Cell Extracts; D002843:Chromatin