[The in vitro inhibitory effect of CD8+ cells from patients with aplastic anemia on normal CFU-GM growth was blocked by cimetidine].
OBJECTIVE
To investigate the effect of histamine type II (H2) receptors on the inhibition of hematopoiesis by CD8+ cells from aplastic anemia (AA) patients.


METHODS
The effects of CD8+ cells and/or cimetidine, alone or in combination, on normal human CFU-GM growth were studied by using CFU-GM assay.


RESULTS
The CD8+ cells from the peripheral blood of AA patients significantly suppressed the growth of normal allogeneic CFU-GM. This inhibitory effect could be blocked by 0.5 x 10(-5) mol/L or 1.0 x 10(-5) mol/L cimetidine. 1.0 x 10(-5) mol/L cimetidine alone slightly inhibited the growth of normal CFU-GM, but 0.5 x 10(-5) mol/L cimetidine did not.


CONCLUSIONS
H2 receptor antagonist cimetidine abolished the suppressive effect of AA patients CDB+ cells on the growth of normal CFU-GM.
Wang
M
M
Department of Hematology, Medical College of Jinan University, Guangzhou 510632.
Yang
L
L
Guo
X
X
Chen
S
S
Liao
J
J
D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000276:immunology* / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism*; D016022:Case-Control Studies; D049109:Cell Proliferation / Q000187:drug effects; D002927:Cimetidine / Q000494:pharmacology*; D005260:Female; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011970:Receptors, Histamine H2 / Q000378:metabolism; D055815:Young Adult
D016827:CD8 Antigens; D006635:Histamine H2 Antagonists; D011970:Receptors, Histamine H2; D002927:Cimetidine