Microbial transformation of tannin-rich substrate to gallic acid through co-culture method.
Modified solid-state fermentation (MSSF) of tannin-rich substrate yielding tannase and gallic acid was carried out using a co-culture of the filamentous fungi, Rhizopus oryzae (RO IIT RB-13, NRRL 21498) and Aspergillus foetidus (GMRB013 MTCC 3557). Powdered fruits of Terminalia chebula and powdered pod cover of Caesalpinia digyna was used in the process and the different process parameters for maximum production of tannase and gallic acid by co-culture method were optimized through media engineering. MSSF was carried out at the optimum conditions of 30 degrees C and 80% relative humidity. The optimal pH and incubation period was 5.0 and 48 h respectively. Through the co-culture technique the maximum yield of tannase and gallic acid was found to be 41.3 U/ml and 94.8% respectively.
Banerjee
Rintu
R
Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur-721302, West Bengal, India. rb@agfe.iikgp.ernet.in
Mukherjee
Gargi
G
Patra
Krushna Chandra
KC
D019149:Bioreactors; D005285:Fermentation; D005658:Fungi; D005707:Gallic Acid / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013634:Tannins / Q000378:metabolism*
D013634:Tannins; D005707:Gallic Acid