Transdermal suctioning technique for serial sampling of interstitial fluid in newborn infants.
BACKGROUND
Use of a suction-induced epidermal mini-erosion for serial sampling of dermal interstitial fluid (IF) in 16 newborn infants with gestational age ranging from 24 to 42 weeks is reported.


RESULTS
The mini-erosion formed reproducibly and electron microscopy showed that the split was located within the epidermis. IF was sampled serially by suctioning during 1-3 days without signs of stress or pain. IF sample volumes (10-50 microl) did not decrease with time. Glucose values in IF and blood were correlated (r(s) = 0.542; p = 0.000). IF glucose was lower than blood glucose on all 3 days. The epidermis regenerated within 4 +/- 0.9 days (mean, SD) after sampling was terminated. There were no complications.


CONCLUSIONS
Serial transdermal sampling was performed without stress or pain in newborn infants. Systemic and temporal differences between glucose measured in IF and blood were observed.
Hansen-Pupp
I
I
Department of Paediatrics, University Hospital, Lund, Sweden. ingrid.pupp@skane.se
Håkansson
K
K
Polberger
S
S
Svedman
P
P
D001786:Blood Glucose / Q000378:metabolism; D004817:Epidermis / Q000502:physiology / Q000648:ultrastructure; D045604:Extracellular Fluid / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007834:Lasers; D008297:Male; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D012038:Regeneration; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism*; D012879:Skin Physiological Phenomena; D013048:Specimen Handling / Q000379:methods*; D013396:Suction / Q000009:adverse effects / Q000379:methods*; D018608:Ultrasonography, Doppler; D014870:Water Loss, Insensible
D001786:Blood Glucose; D005947:Glucose