A unigene catalogue of 5700 expressed genes in cassava.
Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv.manihotis. We report here the single pass sequencing of 11,954 cDNA clones from the 5' ends, including 111 from the 3' ends. Cluster analysis permitted the identification of a unigene set of 5,700 sequences. Sequence analyses permitted the assignment of a putative functional category for 37% of sequences whereas approximately 16% sequences did not show any significant similarity with other proteins present in the database and therefore can be considered as cassava specific genes. A group of genes belonging to a large multigene family was identified. We characterize a set of genes detected only in infected libraries putatively involved in the defense response to pathogen infection. By comparing two libraries obtained from cultivars contrasting in their starch content a group of genes associated to starch biosynthesis and differentially expressed was identified. This is the first large cassava EST resource developed today and publicly available thus making a significant contribution to genomic knowledge of cassava.
Lopez
Camilo
C
Laboratoire Génome et Développement des Plantes, UMR5096, CNRS-Université de Perpignan--Institut de Recherche pour le Développement, 52 Av Paul Alduy, 66860, Perpignan Cedex, France.
Jorge
Véronique
V
Piégu
Benoît
B
Mba
Chickelu
C
Cortes
Diego
D
Restrepo
Silvia
S
Soto
Mauricio
M
Laudié
Michèle
M
Berger
Christel
C
Cooke
Richard
R
Delseny
Michel
M
Tohme
Joe
J
Verdier
Valérie
V
D016000:Cluster Analysis; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D002365:Manihot / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013213:Starch / Q000096:biosynthesis; D014974:Xanthomonas / Q000254:growth & development
D018076:DNA, Complementary; D013213:Starch