Analysis of replication kinetics of the human metapneumovirus in different cell lines by real-time PCR.
Human metapneumovirus (hMPV) is associated with acute respiratory tract disease especially in young children. Using a quantitative real-time TaqMan PCR, we analyzed the replication kinetics of hMPV in different cell lines. Our results indicate that hMPV replicates slightly more efficiently in LLC-MK2 than in Vero cells and poorly in HEp-2 cells.
Deffrasnes
Céline
C
Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec, and Laval University, Canada.
Côté
Stéphanie
S
Boivin
Guy
G
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D007700:Kinetics; D029121:Metapneumovirus / Q000502:physiology*; D018184:Paramyxoviridae Infections / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism; D014709:Vero Cells; D014779:Virus Replication
D019914:Taq Polymerase