Thermoresponsive controlled association of protein with a dynamic nanogel of hydrophobized polysaccharide and cyclodextrin: heat shock protein-like activity of artificial molecular chaperone.
Dynamic CHP-CD nanogels, which consisted of a self-assembly of cholesteryl-group-bearing pullulan (CHP) and beta-cyclodextrin (CD), were characterized by SEC and SEC-MALS methods. The nanogels prevented the thermal aggregation of carbonic anhydrase B (CAB) by selective trapping of the heat-denatured protein. After the complex between the CHP-CD nanogels and CAB was cooled, the enzyme activity of CAB spontaneously recovered upon release from the complex. The dynamic nanogels self-regulated an association of heat denatured protein and dissociation of native protein depending on the concentration of CD. The thermal stability of CAB was improved by thermoresponsive controlled association between the proteins and the artificial molecular chaperone.
Nomura
Yuta
Y
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida-Hommachi, Sakyo-ku, Kyoto 606-8501, Japan.
Sasaki
Yoshihiro
Y
Takagi
Masaki
M
Narita
Tadashi
T
Aoyama
Yasuhiro
Y
Akiyoshi
Kazunari
K
D024401:Carbonic Anhydrase I / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D003505:Cyclodextrins; D005936:Glucans; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D000080385:Nanogels; D011134:Polysaccharides; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; C009109:pullulan; D011094:Polyethyleneimine; D024401:Carbonic Anhydrase I