title: Theoretical studies about the influence of different ring substituents on the nucleophilic ring opening of three-membered heterocycles and possible implications for the mechanisms of cysteine protease inhibitors.
abstract: Epoxides, aziridines, and thiiranes are electrophilic building blocks that are widely used in synthetic organic chemistry. As a result of their reactivity against nucleophiles they are also used as electrophilic "warheads" for irreversible peptidic or peptidomimetic cysteine protease inhibitors. A general feature of these inhibitors is the remarkable higher inhibition potency of derivatives containing a free carboxylic acid in comparison to corresponding esters. In contrast, experimental investigations about the reaction of methyl thiolate with substituted epoxides revealed a contrary reactivity pattern. These studies also gave information about the regioselectivity of such reactions; however, mechanistic studies were not performed. By analyzing the computed energy profiles of the corresponding reactions we investigate the substituent effects (H vs ester vs carboxylic acid) on the kinetics and thermodynamics of the ring opening by the nucleophile methyl thiolate. Our model computations nicely explain experimental results concerning variations in the reactivities and the regioselectivities and indicate different reasons for the increased inhibition potency of three-membered heterocycles containing an acidic substituent. For aziridines an intramolecular water-mediated acid catalysis seems to be the main reason for the high activity of these inhibitors in acidic media. For epoxides and thiiranes this catalysis is not found, confirming the hypothesis that an ionic interaction between negatively charged carboxylate and the histidinium ion of the active site of the proteases mainly causes the high inhibitory activity of the acids compared to the esters.
authors.affiliation: Institut für Organische Chemie and Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Helten
authors.forename: Tanja
authors.initials: T
authors.lastname: Schirmeister
authors.forename: Bernd
authors.initials: B
authors.lastname: Engels
mesh_terms: D001388:Aziridines / Q000737:chemistry*; D002384:Catalysis; D003500:Cyclization; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry*; D007700:Kinetics; D008962:Models, Theoretical; D008968:Molecular Conformation; D013440:Sulfides / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D001388:Aziridines; D015853:Cysteine Proteinase Inhibitors; D004852:Epoxy Compounds; D013440:Sulfides