[Search for new regulatory elements of the Bithorax complex in Drosophila melanogaster].
The Ultrabithorax (Ubx), Abdominal-A (Abd-A), and Abdominal-B (Abd-B) homeotic genes of the Bithorax complex are responsible for the development of body segments in Drosophila melanogaster. Their regulatory region of approximately 300 kb harbors a series of specific enhancers, each regulating expression in a single parasegment. Molecular genetic analysis of the Abd-B gene revealed the regulatory modules MCP, Fab-7, and Fab-8, which contain insulators with adjacent silencers. The present work was aimed at searching for similar regulatory elements in the promoter regions of the Abd-A and Abd-B genes and new regulatory modules in the Abd-B gene region. Four DNA fragments, each containing numerous specific sequences characteristic of regulatory modules, were tested for the ability to suppress transcription of the yellow and white genes and for the interaction with MCP. The DNA fragment of the Abd-A promoter region proved to act as an effective silencer. The fragment of the iab4 domain showed insulator properties. The fragment of the Abd-B promoter region interacted with MCP in providing for communication between the enhancer and promoter of the white gene.
Mel'nik
E S
ES
Kyrchanova
O V
OV
Gruzdeva
N M
NM
Georgiev
P G
PG
D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster; D005091:Exons / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D005801:Genes, Homeobox / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D037941:Silencer Elements, Transcriptional / Q000235:genetics*
D029721:Drosophila Proteins