[The simple gain of templates of rice genomes DNA for PCR].
The solution of alkali-treated fresh rice leaves was used directly as the templates of PCR. The amplified results were stable, reliable, and had no difference compared with that amplified with rice total DNA extracted by common method. The stable results can still be obtained based on the templates kept at 25 degrees for tow weeks, at 4 degrees for three weeks, at -20 degrees for over four months. With this technique, less material and only common reagent are required, which is especially adapted to the screening of the precious transgenic rice in advance and large-scale PCR tests.
Sang
Xian-Chun
XC
Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture Southwest Agricultural University, Chongqing 400716, China. sangxianchun@163.net
He
Guang-Hua
GH
Zhang
Yi
Y
Yang
Zheng-Lin
ZL
Pei
Yan
Y