title: Convenient synthesis of NCS-chromophore metabolite isosteres: binding agents for bulged DNA microenvironments.
abstract: A designed molecule with capacity to bind DNA bulges (20) has been prepared from readily available starting materials. The spirocyclic template was modeled on a metabolite of neocarzinostatin chromophore (NCSi-gb) and is equipped with functionality to enable convenient bioassay. Preliminary studies confirm binding at specific bulged sequences and induction of polymerase-mediated slippage events. The target compound offers a convenient means to study affinity for unique bulged motifs and for use as a molecular biology reagent.
authors.affiliation: Bioorganic and Medicinal Chemistry Laboratories, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Lin
authors.forename: Graham B
authors.initials: GB
authors.lastname: Jones
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
authors.forename: Lizzy
authors.initials: L
authors.lastname: Kappen
authors.forename: Irving H
authors.initials: IH
authors.lastname: Goldberg
mesh_terms: D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA