title: Different gvpC length variants are transcribed within single filaments of the cyanobacterium Planktothrix rubescens.
abstract: Transcripts of the gas vesicle genes gvpA and gvpC were detected in single filaments of the cyanobacterium Planktothrix rubescens using reverse transcription and quantitative real-time PCR. Primers were designed to amplify short sequences within gvpA and three length variants of gvpC. With genomic template DNA, and using Sybr Green to monitor product accumulation, similar amplification efficiencies were observed for each of these genes. The relative copy numbers of gvpC length variants in genomic DNA from five Planktothrix gas vesicle genotypes determined by real-time PCR were similar to those indicated by sequencing the gas vesicle gene clusters. The precipitation of gvp cDNA reverse-transcribed from cellular RNA from single filaments was required before amplification of the gene fragments; without this step it was not possible to detect the accumulation of the expected amplicons by dissociation analysis. Precipitation was also necessary to ensure the generation of product curves that allowed linear regression in an early stage of PCR, a prerequisite for the quantification of low-input cDNA amounts without the need for standard curves. This report shows that different gvpC length variants are transcribed within single Planktothrix filaments, both from laboratory cultures and from natural samples taken from Lake Zurich. This has implications for the efficiency of buoyancy provision by the possible production of gas vesicles of different strengths within individual cyanobacterial filaments. The hypothesis that post-transcriptional regulation may influence the type of protein (GvpC) present in gas vesicles is presented.
authors.affiliation: School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
authors.forename: Sven
authors.initials: S
authors.lastname: Becker
authors.affiliation: School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
authors.forename: Paul K
authors.initials: PK
authors.lastname: Hayes
authors.affiliation: School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Walsby
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011232:Chemical Precipitation; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D005798:Genes, Bacterial; D014644:Genetic Variation; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D011506:Proteins; C038524:gas vesicle protein