Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteins.
The efficiency of viruses in cancer therapy is enhanced by proteins that mediate the fusion of infected cells with their neighbors. It was reported that replication-competent adenovirus particles can spread between nuclei within fusion-generated syncytia. To assess this conjecture, we generated fusogenic adenoviruses that express a balanced ratio of the F and H glycoproteins of measles virus. The viruses displayed enhanced cytotoxicity but largely unchanged replication efficiencies compared to a nonfusogenic virus. Most notably, the virus genomes did not spread through fusion-generated multinuclear cells. Hence, adenovirus replication in syncytia remains largely restricted to initially transduced nuclei.
Horn
German P
GP
Institut für Virologie, Klinikum der Philipps, Universität Marburg, Marburg, Germany.
Vongpunsawad
Sompong
S
Kornmann
Evelyn
E
Fritz
Barbara
B
Dittmer
Dirk P
DP
Cattaneo
Roberto
R
Dobbelstein
Matthias
M
D000256:Adenoviridae / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology*; D015726:Giant Cells; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008561:Membrane Fusion; D011995:Recombination, Genetic; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus