title: Production of nitric oxide, tumor necrosis factor-alpha and interleukin-6 by RAW264.7 macrophage cells treated with lactic acid bacteria isolated from kimchi.
abstract: The present study investigated immunopotentiating activities of lactic acid bacteria isolated from kimchi (KLAB) in vitro. A RAW264.7 macrophage cell line was stimulated with four strains of KLAB and two strains of bifidobacteria, and then the production of nitric oxide (NO) and cytokines--tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6)--was determined. Lactobacillus plantarum, a lactic acid bacteria involved in the latent fermentation of kimchi, was the most effective for the generation of NO, TNF-alpha, and IL-6 in macrophage. All strains generated NO, while for the TNF-alpha and IL-6, only L. plantarum had significant activity. Our results indicate that L. plantarum plays an important role in the immunopotentiating activity of kimchi.
authors.affiliation: Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Haeng Jeon
authors.initials: HJ
authors.lastname: Hur
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D001937:Brassica / Q000382:microbiology*; D005511:Food Handling; D015850:Interleukin-6 / Q000096:biosynthesis*; D007723:Korea; D007934:Leuconostoc / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide