title: Molecular cloning and sequence analysis of cDNAs encoding for Boophilus microplus, Haemaphysalis longicornis and Rhipicephalus appendiculatus actins.
abstract: The nucleotide and deduced amino acid sequences of the actins from ticks, Boophilus microplus, Haemaphysalis longicornis and Rhipicephalus appendiculatus, have been determined. Nucleotide sequence analysis showed open reading frames of 1128-nucleotide-long encoding proteins of 376 amino acids with a predicted molecular weight of 41.82 kDa each. Comparison between the nucleic acid and deduced amino acid sequences as well as structural and phylogenetic analyses of these genes confirmed the high similarity among actins from ticks in comparison to other species.
authors.affiliation: Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Sapporo 060-0818, Hokkaido, Japan. ita@dna.cbiot.ufrgs.br
authors.forename: Itabajara
authors.initials: I
authors.lastname: da Silva Vaz
authors.forename: Saiki
authors.initials: S
authors.lastname: Imamura
authors.forename: Chie
authors.initials: C
authors.lastname: Nakajima
authors.forename: Felipe Cardoso
authors.initials: FC
authors.lastname: de Cardoso
authors.forename: Carlos Alexandre Sanchez
authors.initials: CA
authors.lastname: Ferreira
authors.forename: Gaby
authors.initials: G
authors.lastname: Renard
authors.forename: Aoi
authors.initials: A
authors.lastname: Masuda
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
authors.forename: Misao
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000199:Actins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics; D005260:Female; D026863:Ixodidae / Q000145:classification / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000199:Actins; D018076:DNA, Complementary