Convenient synthesis of NCS-chromophore metabolite isosteres: binding agents for bulged DNA microenvironments.
A designed molecule with capacity to bind DNA bulges (20) has been prepared from readily available starting materials. The spirocyclic template was modeled on a metabolite of neocarzinostatin chromophore (NCSi-gb) and is equipped with functionality to enable convenient bioassay. Preliminary studies confirm binding at specific bulged sequences and induction of polymerase-mediated slippage events. The target compound offers a convenient means to study affinity for unique bulged motifs and for use as a molecular biology reagent.
Lin
Yiqing
Y
Bioorganic and Medicinal Chemistry Laboratories, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA.
Jones
Graham B
GB
Hwang
Geum-Sook
GS
Kappen
Lizzy
L
Goldberg
Irving H
IH
D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
D004247:DNA