[Study of dominant negative effect of the PLZF-RARalpha against the wild-type RARalpha in acute promyelocytic leukemia].
OBJECTIVE
To explore the role of promyelocytic leukemia zinc finger (PLZF) gene on 11q23 and RARalpha gene on 17q21.


METHODS
Eight PLZF/RARalpha expression plasmids with deletions of different PLZF motifs were constructed by using PCR based-method.


CONCLUSIONS
By transient co-transfection, it was confirmed that PLZF/RARalpha had a dominant negative effect against the wild-type RARalpha, the POZ domain of PLZF/RARalpha was responsible for the dominant negative effect and the PLZF/RARalpha may play certain role through a spatial interaction with POZ.
Dong
S
S
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Zhu
J
J
Kuang
S
S
Qiu
J
J
Wang
Z
Z
Chen
S
S
Chen
L
L
D000818:Animals; D045744:Cell Line, Tumor; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000072482:Retinoic Acid Receptor alpha; D016335:Zinc Fingers
D018168:Receptors, Retinoic Acid; D011993:Recombinant Fusion Proteins; D000072482:Retinoic Acid Receptor alpha