[Use of recombinant HBcore antigen for development of the diagnostic test-system].
The plasmid construction expressing recombinant HBc antigen (HbcAg) in Escherichia coli cells under the control of the PL promoter of phage I, was obtained. The specific activity of the antigen thus obtained was controlled by the enzyme immunoassay (EIA) method and compared with the reference system "AxSYM CORE assay" ("Abbott", USA) with four panels of sera (altogether 111 samples). The coincidence of the results of the compared test system with the reference was 96.4%, which made it possible to recommend this genetic construction of recombinant HBcAg for the production of EIA systems.
Puzyrev
V F
VF
Drevtsova
I N
IN
Burkov
A N
AN
Ulanova
T I
TI
D010582:Bacteriophage lambda / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D006509:Hepatitis B / Q000175:diagnosis*; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012680:Sensitivity and Specificity
D006512:Hepatitis B Core Antigens; D011994:Recombinant Proteins