[Construction of Chang Liver cDNA library and immunoscreening of ADAMS related genes].
Total RNA was isolated from Chang Liver cell. The full-length cDNA was synthesized using LD PCR by RNA 5' end switching mechanism, oligo(dT) as primer. SfiI digestion sites were introduced at both 3' and 5' end of cDNA. The cDNA was ligated with gammaTriplEX2 vector and packaged. Twenty-two positive clones were obtained by immunoscreening to the library, they were sequenced and blast in NCBI, one of them was identified a novel gene. It was submitted to GenBank and obtained accession number AY078070. It may lay down a foundation for studying on functions of ADAMs related gene.
Lin
Jun-Tang
JT
Department of Cell Biology, Xinxiang Medical University, Xinxiang 453003, China. linjt2000@163.com
Li
Yu-Chang
YC
Zhang
Hui-Yong
HY
Xu
Cun-Quan
CQ
D051722:ADAM Proteins / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D000906:Antibodies; D018076:DNA, Complementary; D051722:ADAM Proteins