Effects of cycloheximide and actinomycin D on production of acidic nuclear proteins in human lymphocytes activated by phytohemagglutinin.
Previous studies have implicated a role for acidic nuclear proteins in the PHA stimulated activation of human lymphocytes. This study examines the mechanism of synthesis of these proteins. Cycloheximide inhibited their production, while Actinomycin D did not. It is suggested that synthesis proceeds by translation of dormant messenger RNA rather than of newly synthesized messenger RNA.
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Department of Dermatology, Hiroshima Hospital of Japanese National Railways, Futabanosato 3-1-36, Hiroshima, Japan.
D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology*; D006657:Histones / Q000096:biosynthesis; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*
D006657:Histones; D009687:Nuclear Proteins; D010835:Phytohemagglutinins; D011500:Protein Synthesis Inhibitors; D003609:Dactinomycin; D003513:Cycloheximide