Structure of the O-polysaccharide of Hafnia alvei strain PCM 1189 that has hexa- to octasaccharide repeating units owing to incomplete glucosylation.
The O-polysaccharide of Hafnia alvei PCM 1189 consists of D-glucose, D-galactose, D-GalNAc and D-GlcA and lacks the strict regularity. The intact and carboxyl-reduced polysaccharides as well as oligosaccharides obtained by partial acid hydrolysis were studied by chemical and enzymatic analyses, methylation and NMR spectroscopy. The following structure was established for the O-polysaccharide, which is built up of branched hexa- to octasaccharide repeating units differing in the number of lateral glucose residues: [structure: see text] where the glucose residues shown in italics are nonstoichiometric substituents. The repeating units include also a minor O-acetyl group, whose position was not determined.
Katzenellenbogen
Ewa
E
Immunochemistry Department, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland. katzenel@iitd.pan.wroc.pl
Kocharova
Nina A
NA
Zatonsky
George V
GV
Shashkov
Alexander S
AS
Korzeniowska-Kowal
Agnieszka
A
Gamian
Andrzej
A
Bogulska
Maria
M
Knirel
Yuriy A
YA
D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D020611:Hafnia alvei / Q000737:chemistry* / Q000145:classification*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D019081:O Antigens; D011134:Polysaccharides