title: Two-dimensional electrophoresis protein profiling as an analytical tool for human acute leukemia classification.
abstract: Two-dimensional electrophoresis (2-DE) was used to profile the proteins of leukemic cells from 61 cases of akute leukemia (AL) characterized by the French-American-British (FAB) classification. The differentially expressed protein spots were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electrospray ionization-tandem MS (ESI-MS/MS). The distinct protein profiles (DPPs) of AL FAB subtypes were explored successfully, including acute myeloid leukemia (AML), its subtypes (M2, M3, and M5), and acute lymphoid leukemia (ALL), which were homogeneous within different samples of the same subgroup but clearly differed from all other subgroups. We also found a group of proteins differentially expressed between AL cells and normal white blood cells. Among the DPPs of AL subtypes, some proteins have been reported, but most of them were first reported here to mark AML differentiation and to discriminate AML from ALL. These data show that 2-DE protein profiling could be used as an analytical tool for facilitating molecular definition of human AL classification and understanding the mechanism of leukemogensis, and the extension of the present analysis to the currently less well-defined AL will identify additional subgroups and may promote the identification of new targets for specific treatment approaches.
authors.affiliation: Department of Hematology and Oncology, The First Clinical Hospital of Jilin University, Changchun, China.
authors.forename: Jiu-Wei
authors.initials: JW
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Jin
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Kang
authors.forename: Mei-Ru
authors.initials: MR
authors.lastname: Hu
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Bei-Fen
authors.initials: BF
authors.lastname: Shen
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Wang
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007938:Leukemia / Q000145:classification*; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis*; D010449:Peptide Mapping
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins