[Relationship between heparanase mRNA expression in human gastric cancer and its clinicopathological features].
OBJECTIVE
To explore the relationship between heparanase mRNA expression and clinicopathological parameters in human gastric cancer.


METHODS
RT-PCR was used to detect the expression of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues.


RESULTS
Heparanase mRNA was expressed in 29 of the 43 cases of gastric cancer with a positive rate of 67.4%, which was significantly higher than that in adjacent normal gastric tissues (P = 0.013). The expression level was higher in late-stage tumors (stage III and IV) than in early-stage tumors (stage I and II) (P = 0.001), in tumors with invasion to serosa than those without serosal invasion (P = 0.009), in tumors with lymph node metastasis than those without lymph node metastasis (P = 0.018), and in large-sized tumors than in small-sized ones (P = 0.009). The expression was not correlated with patients' age, sex, tumor location, histologic types, differentiation, peritoneal dissemination and liver metastasis (P > 0.05).


CONCLUSIONS
Heparanase might play an important role in the development of invasion and metastasis of gastric cancer.
Chen
Jun-qiang
JQ
Department of Gastrointestinopancreatic Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Zhan
Wen-hua
WH
He
Yu-long
YL
Peng
Jun-sheng
JS
Wang
Jian-ping
JP
Chen
Zheng-xuan
ZX
Huang
Yi-hua
YH
Cai
Shi-rong
SR
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology*; D000099175:Heparanase
D005966:Glucuronidase; D012333:RNA, Messenger; D000099175:Heparanase