title: [Evolution of partial promoter region of HMW glutenin genes from E and E1 genome of Agropyron elongatum].
abstract: The partial promoter regions of HMW glutenin subunit genes were cloned form the genomes E (in diploid Agropyron elongatum) and E1 (in tetraploid Agropyron elongatum) by PCR approach. There was only one nucleotide acid difference in the promoter sequences of x-type subunits between the two genomes; moreover, the promoter sequences of the two y-type subunits were completely identical. Although these promoter regions were very similar to each other, differences still existed in sequence size and the kind of nucleotide acid between the x-type and y-type subunits. It was speculated that the E1 genome in tetraploid Agropyron elongatum was probably originated from E genome in diploid species. The comparisons of these subunits with some of those from A, B, D and G genome of Triticeae demonstrated that the sequences of their partial promoter regions were conserved and shared a high homology more than 90%. The phylogenetic analysis based on the sequences in this region indicated that the y-type HMW glutenin subunits of Agropyron elongatum species were different from other subunits, whereas the x-type subunits of them were most closely related to that from the B genome.
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Deng
authors.forename: Xiang-Qi
authors.initials: XQ
authors.lastname: Zhang
mesh_terms: D031702:Agropyron / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D004171:Diploidy; D019143:Evolution, Molecular; D016678:Genome; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011123:Polyploidy; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018744:DNA, Plant; D005983:Glutens; C037302:glutenin