[Establishment of a new method for early embryo culture in vitro].
A new method, nest culture method, was developed in this study. The culture effects of different methods for embryo culture in vitro were compared. The results showed that whether the suspension in phi35mm dish in nest culture method was covered with mineral oil or not, the developmental rates of embryos had no significant difference. Compared to the nest culture method, the developmental rates of embryos in Brinster's method were significant lower. However, all embryos cultured in the single dish in which the suspensions were not covered with mineral oil were blocked at 2-cell stage. The nest culture method is an effective method for early embryo culture in vitro.
Zhao
Yong-Zhen
YZ
Northwest Science and Technology University of Agriculture and Forestry, Yangling, Shanxi 712 100, China.
Zheng
Xin-Min
XM
Cao
Bin-Yun
BY
Wei
Qing-Xin
QX
Li
Li
L
Wei
Yan
Y
Li
Ju-Xue
JX
D000818:Animals; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D051379:Mice; D008899:Mineral Oil / Q000494:pharmacology
D003470:Culture Media; D008899:Mineral Oil