[Study of polymorphism at new Y-STR DYS605 in a Chinese Han population of Shanxi].
We study the polymorphism at DYS605 ,a new tetranucleotide Y-STR locus,in a Chinese Han population of Shanxi to meet the need of more genetic markers in forensic practice and genetic analysis. DNA were extracted from 128 unrelated male venous blood, and amplified using GDB primers. PCR products were detected using non-denaturing polyacrylamide gel electrophoresis and silver staining. Five alleles named 22,21,20,19,18 were observed with frequency of 0.0156;0.1797;0.4531;0.2891;0.0625. PCR products were not found in female DNA. Using a long enough gel for a long electrophoresis time is strongly encouraged because the rung between allele 20 and allele19 is smaller than expected. Allele sequences show that the repetitive units of DYS605 were composed with the variant units and non-variant units.
Zhang
Geng-Qian
GQ
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030 001, China. zgengqian@163.com
Wang
Yan
Y
Zhang
Yan-Xia
YX
Xu
Xiao-Li
XL
Xing
Xiao-Ping
XP
Wang
Ying-Yuan
YY
Yun
Ke-Ming
KM
D000483:Alleles; D044466:Asian People; D002681:China / Q000208:ethnology; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic