[Novel molecular marker systems--SRAP and TRAP and their application].
SRAP and TRAP are two kinds of newly developed molecular marker systems with the advantages of simplicity, high throughput, numerous co-dominant makers, highly reproducibility and ready to sequence, especially preferential targeting ORFs. The principle and protocol of SRAP and TRAP, which were developed in Brassica and Helianthus crops firstly, are introduced in the paper. Primer design is a key step for SRAP and TRAP, and some F-and R-primers were developed. Unlike SRAP technique, the TRAP requires cDNA or EST sequence information for fixed primer development. The annealing temperature is 35 degrees in the first 5 cycles and 50 degrees in the subsequent 30~35 cycles. The amplicons can be separated by polyacrylamide or agarose gel, and detected by autoradiography, silver or EB staining. SRAP and TRAP are applied in germplasm genetic diversity analysis, genetic map including transcriptome map construction, important trait gene tagging and gene cloning in many crops.
Liu
Li-Wang
LW
National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing 210 095, China. nauliulw@njau.edu.cn
Gong
Yi-Qin
YQ
Huang
Hao
H
Zhu
Xiao-Wen
XW
D000135:Acid Phosphatase / Q000235:genetics*; D001937:Brassica / Q000235:genetics*; D001947:Breeding; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D005091:Exons; D020224:Expressed Sequence Tags; D005819:Genetic Markers / Q000235:genetics*; D006368:Helianthus / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D000071681:Tartrate-Resistant Acid Phosphatase
D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D005819:Genetic Markers; D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase