title: Generation of pluripotent stem cells from neonatal mouse testis.
abstract: Although germline cells can form multipotential embryonic stem (ES)/embryonic germ (EG) cells, these cells can be derived only from embryonic tissues, and such multipotent cells have not been available from neonatal gonads. Here we report the successful establishment of ES-like cells from neonatal mouse testis. These ES-like cells were phenotypically similar to ES/EG cells except in their genomic imprinting pattern. They differentiated into various types of somatic cells in vitro under conditions used to induce the differentiation of ES cells and produced teratomas after inoculation into mice. Furthermore, these ES-like cells formed germline chimeras when injected into blastocysts. Thus, the capacity to form multipotent cells persists in neonatal testis. The ability to derive multipotential stem cells from the neonatal testis has important implications for germ cell biology and opens the possibility of using these cells for biotechnology and medicine.
authors.affiliation: Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Mito
authors.initials: M
authors.lastname: Kanatsu-Shinohara
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Momoko
authors.initials: M
authors.lastname: Yoshimoto
authors.forename: Narumi
authors.initials: N
authors.lastname: Ogonuki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miki
authors.forename: Shiro
authors.initials: S
authors.lastname: Baba
authors.forename: Takeo
authors.initials: T
authors.lastname: Kato
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kazuki
authors.forename: Shinya
authors.initials: S
authors.lastname: Toyokuni
authors.forename: Megumi
authors.initials: M
authors.lastname: Toyoshima
authors.forename: Ohtsura
authors.initials: O
authors.lastname: Niwa
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oshimura
authors.forename: Toshio
authors.initials: T
authors.lastname: Heike
authors.forename: Tatsutoshi
authors.initials: T
authors.lastname: Nakahata
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Ishino
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
authors.forename: Takashi
authors.initials: T
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D047108:Embryonic Development; D017353:Gene Deletion; D018392:Genomic Imprinting; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D013737:Testis / Q000166:cytology*; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53