title: Liquid-liquid extraction of alpha-lactalbumin using reverse micellar organic solvent.
abstract: The liquid-liquid extraction of alpha-lactalbumin based on reverse micellar organic solvents was investigated. Forward extraction of the protein in the reverse micellar organic phase from aqueous feed solutions was strongly dependent on the initial pH of the feed solution and the complete forward extraction of 0.03 mM alpha-lactalbumin was successfully achieved at pH 6.0. The forward extraction percentage steeply decreased with increasing KCl concentration, while in the NaCl system the forward extraction was independent on the salt concentration below 1 M. From the circular dichroic measurement, higher order structure of the recovered alpha-lactalbumin through the extraction process was well preserved.
authors.affiliation: Department of Chemical Engineering, Nara National College of Technology, 22 Yata, Yamato-Koriyama, Nara 639-1080, Japan. naoe@chem.nara-k.ac.jp
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Naoe
authors.forename: Kazuki
authors.initials: K
authors.lastname: Noda
authors.forename: Taiji
authors.initials: T
authors.lastname: Konishi
authors.forename: Mikio
authors.initials: M
authors.lastname: Kawagoe
authors.forename: Masanao
authors.initials: M
authors.lastname: Imai
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007768:Lactalbumin / Q000302:isolation & purification*; D008823:Micelles; D008892:Milk / Q000737:chemistry; D009994:Osmolar Concentration; D012997:Solvents
chemical_list: D007202:Indicators and Reagents; D008823:Micelles; D012997:Solvents; D007768:Lactalbumin