[Study on cryopreservative methods for dendritic cells derived from K562 cell line].
To investigate the biological properties of cryopreserved dendritic cell (DC) derived from K562 cell line, thus to provide a simple, quick and efficient preservative method of DC for infusion of DC to patients with leukemia after complete remission, fresh DC induced from K562 cell line (K562-DC) was frozen in -196 degrees C liquid nitrogen and -80 degrees C mechanical freezer by method of steps (RPMI 1640 with 10% DMSO, 20% FCS as cryopreservatives), and thawed in different time, respectively. Survivals of cryopreserved and fresh K562-DC, expression of surface antigens, stimulating index (SI) and cytotoxic eliminating rate were detected. The results of fresh induced cells were compared with that of cryopreserved ones. The results showed that before and after frozen in liquid nitrogen, the morphological characteristics of K562-DC had no distinct change; and both their expression rates of surface molecular and capacity to stimulate allogeneic lymphocyte had no statistic significance (P > 0.05). In addition, there were no differences in terms of viability, stimulatory capacity and cytotoxicity of K562-DC from two ways for less than one month (P > 0.05), but there were differences when frozen for more than one month (P < 0.05). It is concluded that there is no significant difference when frozen less than one month between liquid nitrogen and -80 degrees C freezer; but when time is more than one month, K562-DC frozen in -196 degrees C liquid nitrogen is better than that in -80 degrees C freezer.
Meng
Dong-Mei
DM
Department of Hematology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, 266003, China. meng-dongmeiqd@yahoo.com.cn
Zhao
Chun-Ting
CT
Wang
Bao-Zhong
BZ
Yang
Jie
J
Chen
Bing
B
D000954:Antigens, Surface / Q000276:immunology; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology*; D006801:Humans; D020014:K562 Cells; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000473:pathology; D013997:Time Factors
D000954:Antigens, Surface