[Cloning and sequencing of the introns of UCP gene and construction of molecular phylogenetic tree in chicken].
The UCP genes were the newly discovered genes that can increase the energy expenditure and involve in the metabolism of fat and regulation of energy. Four pairs of primers in chicken UCP exon region were designed to amplify the introns of chicken UCP gene according to the splice ways of the mouse UCP2 gene (Accession No.AF096288). The sequence results showed that the chicken UCP gene also had five GT-AG type introns. The molecular phylogenetic tree was constructed based on the sequence of cds, intron 2 and intron 3 region, respectively. The phylogenetic tree based on the UCP cds region was consistent with the species phylogenetic tree. This result implicated that UCP gene can be regarded as the useful gene for the study of animal phylogenesis. On the contrast, the phylogenetic tree based on the intron 2 and intron 3 region was different from the species phylogenetic tree, which showed that the evolution of intron and cds region is different.
Zhao
Jian-Guo
JG
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. zhaojervis@hotmail.com
Li
Hui
H
Wang
Qi-Gui
QG
Wang
Yu-Xiang
YX
Tang
Zhi-Quan
ZQ
D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D007438:Introns / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D000071245:Mitochondrial Uncoupling Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D030161:Avian Proteins; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; C422907:mitochondrial uncoupling protein, chicken; D004247:DNA