Morphometrical analysis of immunohistochemical reaction of inflammatory infiltrate in chronic thyroiditis.
The aim of the study was to quantitatively evaluate B and T lymphocytes and macrophages, based on immunohistochemical investigations (CD43, CD20, CD8 and CD68) of chronic focal and Hashimoto thyroiditis. A new method of image analysis was applied, based on spatial visualization of the antigens reactivity. The obtained results indicated that the numbers of lymphocytes, in particular of cytotoxic T lymphocytes, and of macrophages increased with the progress of inflammatory process. Quantitative measurements of the markers made the results more objective and supported pathomorphological diagnosis.
Nieruchalska
E
E
Laboratory of Morphometry and Medical Image Processing, University of Medical Sciences, Poznań, Poland.
Kaczmarek
E
E
Jarmołowska-Jurczyszyn
D
D
Majewski
P
P
D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D002908:Chronic Disease; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D013965:Thyroidectomy; D013966:Thyroiditis / Q000276:immunology / Q000473:pathology* / Q000601:surgery
D015703:Antigens, CD