title: [Diagnosis of hepatitis E virus infection in sporadic acute hepatitis].
abstract: The solid-phase enzyme immunoassay (EIA) was used for detecting anti-HEV immunoglobulin G (IgG) and M (IgM) by using a recombinant chimeric antigen (ORF3-2) from the open reading frame (ORF) 2 and 3 of hepatitis E virus gene. These sera were collected from the patients with sporadic acute viral hepatitis. The positive rate for anti-HEV IgG was 15.22% (7) among 46 patients with acute hepatitis, 5 of the 7 IgG positive sera were also positive (71.4%) for anti-HEV IgM. 91.8% (93) and 76.4% (78) were positive separately for anti-HEV IgG and IgM among 102 patients of suspected HEV infection (these patients were negative for anti-HAV, anti-HBV and anti-HCV), about 2/3 patients of HEV infection detected were simultaneously HEV-IgG and IgM positive. In control group of 98 normal persons, 4 (4.08%) were positive for anti-HEV IgG, but none were IgM positive. Paired sera of the patients in the acute phase and convalescent phase were detected, the titers of anti-HEV IgG with 4-16 fold decrease were seen in 6 cases and with 2-fold rise in 1 case.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: S
authors.initials: S
authors.lastname: Bi
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: S
authors.initials: S
authors.lastname: Liao
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Zhan
authors.forename: C
authors.initials: C
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D000914:Antibodies, Viral / Q000276:immunology; D016751:Hepatitis E / Q000175:diagnosis* / Q000276:immunology* / Q000821:virology; D016752:Hepatitis E virus / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M