title: Novel aspects of intrinsic and extrinsic aging of human skin: beneficial effects of soy extract.
abstract: Biochemical and structural changes of the dermal connective tissue substantially contribute to the phenotype of aging skin. To study connective tissue metabolism with respect to ultraviolet (UV) exposure, we performed an in vitro (human dermal fibroblasts) and an in vivo complementary DNA array study in combination with protein analysis in young and old volunteers. Several genes of the collagen metabolism such as Collagen I, III and VI as well as heat shock protein 47 and matrix metalloproteinase-1 are expressed differentially, indicating UV-mediated effects on collagen expression, processing and degradation. In particular, Collagen I is time and age dependently reduced after a single UV exposure in human skin in vivo. Moreover, older subjects display a lower baseline level and a shorter UV-mediated increase in hyaluronan (HA) levels. To counteract these age-dependent changes, cultured fibroblasts were treated with a specific soy extract. This treatment resulted in increased collagen and HA synthesis. In a placebo-controlled in vivo study, topical application of an isoflavone-containing emulsion significantly enhanced the number of dermal papillae per area after 2 weeks. Because the flattening of the dermal-epidermal junction is the most reproducible structural change in aged skin, this soy extract appears to rejuvenate the structure of mature skin.
authors.affiliation: Beiersdorf AG, Paul Gerson Unna Skin Research Center, Hamburg, Germany.
authors.forename: Kirstin M
authors.initials: KM
authors.lastname: Südel
authors.forename: Kirsten
authors.initials: K
authors.lastname: Venzke
authors.forename: Heiko
authors.initials: H
authors.lastname: Mielke
authors.forename: Ute
authors.initials: U
authors.lastname: Breitenbach
authors.forename: Claudia
authors.initials: C
authors.lastname: Mundt
authors.forename: Sören
authors.initials: S
authors.lastname: Jaspers
authors.forename: Urte
authors.initials: U
authors.lastname: Koop
authors.forename: Kirsten
authors.initials: K
authors.lastname: Sauermann
authors.forename: Elke
authors.initials: E
authors.lastname: Knussman-Hartig
authors.forename: Ingrid
authors.initials: I
authors.lastname: Moll
authors.forename: Günther
authors.initials: G
authors.lastname: Gercken
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Young
authors.forename: Franz
authors.initials: F
authors.lastname: Stäb
authors.forename: Horst
authors.initials: H
authors.lastname: Wenck
authors.forename: Stefan
authors.initials: S
authors.lastname: Gallinat
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects / Q000378:metabolism*; D055513:Connective Tissue Growth Factor; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D017874:Immediate-Early Proteins / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D010641:Phenotype; D010936:Plant Extracts / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000502:physiology; D013025:Glycine max / Q000737:chemistry*
chemical_list: C526317:CCN2 protein, human; D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010936:Plant Extracts; D055513:Connective Tissue Growth Factor; D006820:Hyaluronic Acid; D003094:Collagen