[Polymorphism analysis of the GOLA-DRB3 gene digested with HaeIII in Mongolian goat and Kazakh goat].
The exon2 of GOLA-DRB3 gene was amplified and a uniform fragment of 285 bp was obtained in Mongolian Goat and Kazakh Goat. The 285bp PCR product was digested with restriction endomuclease HaeIII and genetic polymorphism was investigated by PCR-RFLP. Seventeen kinds of genotypes were found in two populations,which were controlled by seven alleles. There are significant differences in some genotypic frequencies and gene frequencies between the two populations (P<0.10, P<0.05, P<0.01); The results of chi(2) test showed that genotypes of GOLA-DRB3 gene in two populations did not fit with Hardy-Weinberg equilibrium (P<0.01).
Sun
Dong-Xiao
DX
College of Animal Science And Technology, China Agricultural University, Beijing 100094, China. sundx@cau.edu.cn
Zhang
Yuan
Y
Li
Ning
N
D000483:Alleles; D000818:Animals; D016009:Chi-Square Distribution; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000145:classification / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases