title: Induction of apoptosis in a human lymphoma cell line by hydrophobic peptide fraction separated from anchovy sauce.
abstract: Peptide fractions showing anticancer activity were isolated from anchovy sauce, and their abilities to induce apoptosis in a human lymphoma cell (U937) were determined by biochemical and flow cytometric methods. The butanol extract (fraction Aob) from the desalted hydrophobic peptide fraction (Ao) separated from anchovy sauce by HP-20 adsorption chromatography was found to possess strong antiproliferative activity against U937 by inducing apoptosis. Treatment of U937 with Aob resulted in sub-G1 peak representing apoptosis in the cell cycle analysis, and apoptotic DNA fragmentation. Antiproliferative activity of the peptide fraction further purified successively by silica gel chromatography, TLC, and reversed-phase HPLC increased proportionally with the purification fold. The peptide fraction having antiproliferative activity was found to be composed of Ala and Phe, and its molecular weight was estimated to be 440.9 Da.
authors.affiliation: Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-744, South Korea.
authors.forename: Young Gon
authors.initials: YG
authors.lastname: Lee
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005399:Fishes; D005511:Food Handling; D006801:Humans; D008223:Lymphoma; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D000970:Antineoplastic Agents; D010455:Peptides; D014020:Tissue Extracts