Ascorbate restores lifespan of superoxide-dismutase deficient yeast.
Yeast (Saccharomyces cerevisiae) mutants lacking CuZn-superoxide dismutase (CuZnSOD) are hypersensitive to oxygen and have significantly decreased replicative life span. Both these defects can be ameliorated by exogenous ascorbate. The effect of ascorbate on life span is complicated by auto-oxidation of its compound in the medium. If negative effects of auto-oxidation are prevented by exchange of the medium, ascorbate prolongs not only mean but also maximal replicative life span of the yeast in the atmosphere of air and of pure oxygen. These results demonstrate that life span shortening due to the lack of a vital antioxidant enzyme can be ameliorated by a low-molecular weight antioxidant.
Krzepiłko
Anna
A
Zamość College of Agriculture, ul. Szczebrzeska 102, PL 22-400 Zamość, Poland.
Swieciło
Agata
A
Wawryn
Jarosław
J
Zadrag
Renata
R
Kozioł
Sabina
S
Bartosz
Grzegorz
G
Biliński
Tomasz
T
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D009154:Mutation; D010100:Oxygen / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D013482:Superoxide Dismutase / Q000172:deficiency*; D013481:Superoxides
D000975:Antioxidants; D013481:Superoxides; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D010100:Oxygen