Method for estimation of toxic endotoxin in inactivated salmonella vaccine in D-galactosamine-sensitized mice.
We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD50 in the mouse safety test (r=0.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
Takikawa
Noriyasu
N
Research Center for Biologicals, The Kitasato Institute, 6-111 Arai, Kitamoto, Saitama 364-0026, Japan.
Kawahara
Kazuyoshi
K
Sawata
Akira
A
Kume
Katsumi
K
D000818:Animals; D001428:Bacterial Vaccines / Q000737:chemistry*; D004731:Endotoxins / Q000032:analysis*; D005688:Galactosamine; D007928:Lethal Dose 50; D008033:Limulus Test / Q000379:methods*; D051379:Mice / Q000276:immunology*; D008809:Mice, Inbred C3H; D012475:Salmonella
D001428:Bacterial Vaccines; D004731:Endotoxins; D005688:Galactosamine