Liquid chromatography-tandem mass spectrometry for the determination of lithospermic acid B in rat serum.
A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/ MS) method for the determination of lithospermic acid B (LSB) in rat serum was developed. LSB and internal standard, 7-hydroxy-3-phenyl-chromen-4-one (HPC) were extracted from rat serum with methyl-tert-butyl ether at acidic pH and analyzed on a Luna C8 column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 6.5) (50:50, v/v). The analytes were detected using a negative electrospray ionization tandem mass spectrometry in the multiple-reaction-monitoring mode. The standard curve was linear (r2= 0.997) over the concentration range of 10.0-500 ng/mL. The coefficient of variation and relative error for intra- and interassay at three QC levels were 1.1 approximately 6.2% and -10.3 approximately -2.7%, respectively. The recovery of LSB from serum sample ranged from 73.2 to 79.5%, with that of HPC (internal standard) being 75.1 %. The lower limit of quantification for LSB was 10 ng/mL using 50 microL of serum sample.
Kim
Hui-Hyun
HH
College of Pharmacy and Phytofermentation Research Center, Wonkwang University, Iksan 570-749, Korea.
Ji
Hye Young
HY
Lee
Hye Won
HW
Kim
Youn-Chul
YC
Sohn
Dong Hwan
DH
Lee
Hye Suk
HS
D000818:Animals; D001572:Benzofurans / Q000097:blood* / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D053630:Depsides; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001572:Benzofurans; D053630:Depsides; C076944:salvianolic acid B