title: Temperature and enantioseparation by macrocyclic glycopeptide chiral stationary phases.
abstract: Seventy-one chiral compounds were separated on four macrocyclic glycopeptide chiral selectors: teicoplanin, its aglycone, ristocetin A and vancomycin, using three possible separation modes: reversed phase with methanol/buffer mobile phases, normal phase with hexane/ethanol mobile phases and polar ionic mode (PIM) with 100% methanol mobile phase with trace amounts of acid and/or base. These 148 separations were studied in a 5-45 degrees C temperature range. Peak efficiencies always increased with temperature, but in only 17% of the separations studied a small increase of the enantioresolution factor was observed. In the majority (83%) of the cases, the enantioresolution decreased or even vanished when temperature increased. All 148 Van't Hoff plots were linear showing that the selector did not change in the temperature range studied. The calculated enthalpy and entropy variations showed that the interaction of the solute with the stationary phase was always enthalpy driven with normal and reversed mobile phases. It could be enthalpy as well as entropy driven with PIM mobile phases strongly dependent on the solute. The plots of delta(deltaH) versus delta(deltaS) were linear in most cases (enthalpy entropy compensation). This observation cannot be used to give clear information on chiral recognition mechanisms, but it allowed identifying specific stationary phase-solute interactions because the points corresponding to the respective thermodynamic parameters were clearly delineated from the general compensation lines.
authors.affiliation: Laboratoire des Sciences Analytiques, Université de Lyon 1, CNRS 5180, 69622 Villeurbanne, France. berthod@univ-lyon1.fr
authors.forename: Alain
authors.initials: A
authors.lastname: Berthod
authors.forename: Brian Lingfeng
authors.initials: BL
authors.lastname: He
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Beesley
mesh_terms: D006020:Glycopeptides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013696:Temperature; D013816:Thermodynamics
chemical_list: D006020:Glycopeptides