title: DNA-binding properties of cosmomycin D, an anthracycline with two trisaccharide chains.
abstract: Cosmomycin D (CosD) is the major constituent fraction isolated from a culture of Streptomyces olindensis ICB20. The ability of this compound to intercalate with double-stranded DNA was studied by gel mobility shift assays and electrospray ionization mass spectrometry (ESI-MS). ESI-MS experiments showed that the complex of CosD with 16-mer double-stranded DNA was at least as stable as a complex of daunorubicin with the same DNA sequence. This is the first study showing DNA binding properties of an anthracycline containing a beta-rhodomycinone aglycone chromophore O-linked to two trisaccharide chains.
authors.affiliation: Institute of Biomedical Sciences, University of Sao Paulo, SP, CEP 005508-900, Brazil.
authors.forename: Renata L A
authors.initials: RL
authors.lastname: Furlan
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Watt
authors.forename: Leandro M
authors.initials: LM
authors.lastname: Garrido
authors.forename: Gustavo P
authors.initials: GP
authors.lastname: Amarante-Mendes
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nur-e-alam
authors.forename: Jurgen
authors.initials: J
authors.lastname: Rohr
authors.forename: Alfredo
authors.initials: A
authors.lastname: Braña
authors.forename: Carmen
authors.initials: C
authors.lastname: Mendez
authors.forename: Jose A
authors.initials: JA
authors.lastname: Salas
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Sheil
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Beck
authors.forename: Gabriel
authors.initials: G
authors.lastname: Padilla
mesh_terms: D018943:Anthracyclines / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D018943:Anthracyclines; D004247:DNA; C052002:cosmomycin D