A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra.
Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here. These two new sequences display the intra-residue Ca-CO correlation selectively, while in the original sequence both the inter- and the intra-residue correlations were present. In addition, one of the two variants benefits from an improved sensitivity. Both sequences are a useful complement to the CANCO sequence for facile sequence-specific protein assignment by protonless NMR.
Bermel
Wolfgang
W
Bruker BioSpin GmbH, Rheinstetten, Germany.
Bertini
Ivano
I
Felli
Isabella C
IC
Pierattelli
Roberta
R
Vasos
Paul R
PR
D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D002135:Calcium-Binding Proteins; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; C034733:oncomodulin