[Identification for genetically modified maize T14/T25 with real time fluorescent PCR method].
To identify genetically modified (GM) maize T14/T25 lines, a real-time fluorescent PCR (RTF PCR) assay was performed in this study. Primers and Taqman probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorescent (RTF) PCR and PCR assays. The RTF PCR method was established to detect and identify GM maize lines. The results show that the TaqMan probe could identify T14/T25 maize used, while other GM and NO-GM maize didn't be detected. The RTF PCR could be a new method for detecting other genetically modified organism.
Cao
Ji-Juan
JJ
Shenyang Agriculture University, Shenyang 110 161, China. cjj0909@sina.com
Qin
Wen
W
Zhu
Shui-Fang
SF
Cao
Yuan-Yin
YY
D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant