Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells.
Although basic fibroblast growth factor (FGF2) is generally included in the media for maintenance of human embryonic stem cells (hESCs), the action of FGF2 in these cells has not been well defined. Here, we determined the roles of FGF2 in maintaining hESC self-renewal. Withdrawal of FGF2 from the media led to acquisition of typical differentiated characteristics in hESCs. In the presence of FGF2, which is normally required for proliferation in an undifferentiated state, inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt/PKB signal stimulated differentiation and attenuated the expression of extracellular matrix (ECM) molecules. We suggest that FGF2 maintains hESC self-renewal by supporting stable expression of ECM molecules through activation of the PI3K/Akt/PKB pathway.
Kim
Sun Jong
SJ
Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, 701-4 Naebalsandong, Kangseogu, Seoul 157-280, South Korea.
Cheon
Seon Hye
SH
Yoo
Seung Jun
SJ
Kwon
Jinie
J
Park
Jong Hyuk
JH
Kim
Chul Geun
CG
Rhee
Kunsoo
K
You
Seungkwon
S
Lee
Joo-Yong
JY
Roh
Sung Il
SI
Yoon
Hyun Soo
HS
D064413:3-Phosphoinositide-Dependent Protein Kinases; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002867:Chromones / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D009025:Morpholines / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D000081082:Phosphoinositide-3 Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt; D017474:Receptors, Laminin / Q000502:physiology; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D064413:3-Phosphoinositide-Dependent Protein Kinases; D002867:Chromones; D016326:Extracellular Matrix Proteins; D016222:Fibroblast Growth Factor 2; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D017474:Receptors, Laminin; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C494918:AKT1 protein, human