Heme oxygenase-1: redox regulation of a stress protein in lung and cell culture models.
Reactive oxygen species (ROS) may contribute to tissue damage in many pathophysiological conditions and participate in physiological signaling processes. The mechanisms by which cells sense prooxidant states, and activate signaling pathways leading to adaptive responses, remain incompletely understood. Bacteria contain several transcriptional regulators (e.g., OxyR) and a low-molecular-weight heat shock protein (HSP33), whose activity increases upon oxidation of critical sulfhydryl residues. These proteins participate in cellular adaptation to oxidative stress. In higher organisms, heme oxygenase-1 (HO-1) has been widely studied as a model for redox-regulated gene expression. Expression of HO-1 responds to chemical and physical agents that directly or indirectly generate ROS. Depletion of cellular reduced glutathione may act as a signal for HO-1 transcriptional activation. Furthermore, antioxidants and metal-chelating compounds can modulate HO-1 expression. Several signaling molecules (e.g., mitogen-activated protein kinases), transcriptional regulators (activator protein-1, NF-E2-related factor-2, hypoxia-inducible factor-1, Bach-1), as well as two enhancer regions in the ho-1 5' regulatory region, participate in the regulation of the ho-1 gene. HO-1 protein expression can occur in the lung in response to oxidative stress associated with infection, altered oxygen tension, and inflammatory diseases. HO-1 remains widely regarded as a protective mechanism against oxidative tissue injury.
Ryter
Stefan W
SW
Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, The University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA. Ryters@upmc.edu
Choi
Augustine M K
AM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000378:metabolism; D002478:Cells, Cultured; D004220:Disulfides; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins / Q000378:metabolism; D005658:Fungi / Q000502:physiology*; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D005978:Glutathione / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051547:Heme Oxygenase-1; D006801:Humans; D008168:Lung / Q000473:pathology*; D008565:Membrane Proteins; D008954:Models, Biological; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D015398:Signal Transduction; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D000975:Antioxidants; D001426:Bacterial Proteins; D004220:Disulfides; D005656:Fungal Proteins; D008565:Membrane Proteins; D016877:Oxidants; D017382:Reactive Oxygen Species; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D005978:Glutathione