[Effect of MT1M gene on the cell cycle and signaling pathway of Hep-G2].
OBJECTIVE
To investigate the possible roles of MT1M gene on the cell cycle and signaling pathway of Hep-G2.


METHODS
Hep-G2 human hepatoma cells made by transfection with expressible MT1M gene, and the cell cycle was detected by flow cytometry, and the signaling pathway was measured by dual luciferase assay in Hep-G2 cells.


RESULTS
MT1M gene was able to induce changes of the cell cycle and the activation of NF-kappaB pathway in Hep-G2 cells.


CONCLUSIONS
MT1M gene may affect the cell cycle in Hep-G2 and activate the NF-kappaB-dependent transcription.
Sun
Luhong
L
Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Zhang
Xiran
X
Kong
Yahui
Y
Yu
Long
L
D045744:Cell Line, Tumor; D005434:Flow Cytometry; D016195:G2 Phase / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008668:Metallothionein / Q000235:genetics*; D016328:NF-kappa B / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection
D016328:NF-kappa B; D008668:Metallothionein