title: [Effect of factors on callus biomass and synthetic mass of hypericin in Hypericum perforatum].
abstract: OBJECTIVE
  To study the effect of several factors on the quantity of hypericin in H. perforatum callus.
  
  
  METHODS
  High efficiency liquid phase chromatography and plant tissue culture were applied.
  
  
  CONCLUSIONS
  When the ratio of nitro-nitrogen to amina-nitrogen is 3:1, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
authors.forename: Ming-shu
authors.initials: MS
authors.lastname: Xu
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Mei-lan
authors.initials: ML
authors.lastname: Chen
authors.forename: He-chun
authors.initials: HC
authors.lastname: Ye
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
mesh_terms: D000873:Anthracenes; D003470:Culture Media; D020902:Hypericum / Q000254:growth & development* / Q000378:metabolism; D008358:Mannose / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology*; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D011205:Povidone / Q000031:analogs & derivatives / Q000494:pharmacology; D046509:Tissue Culture Techniques
chemical_list: D000873:Anthracenes; D003470:Culture Media; C077842:polyvinylpolypyrrolidone; D010569:Perylene; C004965:hypericin; D011205:Povidone; D009584:Nitrogen; D008358:Mannose