[Effects of ampelopsin on invasion and metastasis of B16 mouse melanoma in vivo and in vitro].
OBJECTIVE
To investigate the effects of ampelopsin on B16 melanoma's invasion and metastasis in vivo and in vitro.


METHODS
B16 mouse melanoma cells were injected into C57BL/6 mouse via tail lateral vein, which subsequently colonized into the animal lungs to form an experimental pulmonary metastasis of tumor cell. The ampelopsin was administered at 3 dosages by intraperitoneal injection daily for 18 days from the day before the cells injection. The B16 mouse melanoma cells were exposed to ampelopsin for 3 days. The effects of ampelopsin on invasion, migration and adhesion of B16 melanoma cells were evaluated with Transwell chambers or attachment with polycarbonate filters and reconstituted basement membrane (Matrigel).


RESULTS
The number of metastases in the animals that were given ampelopsin 150, 200, and 250 mg x kg(-1) x d(-1) was significantly reduced as compared to the vehicle control (P<0.05), and the inhibition rates were 30.97%, 40.58%, and 61.16%, respectively. The ability of the ampelopsin treated B16 cells to invade the reconstituted basement membrane was decreased significantly (P<0.01), and the inhibition rates were 36.06%, 59.58%, and 79.09% at 20 micromol x L(-1), 40 micromol x L(-1) and 80 micromol x L(-1) concentration, respectively. Ampelopsin could also inhibit B16 cells migration through PVPF in the Transwell chambers, and the inhibition rates were 51.59%, 56.51%, and 66.75% at 20 micromol x L(-1), 40 micromol x L(-1) and 80 micromol x L(-1), respectively (P<0.01). The ability of adhesion of the B16 cells by ampelopsin treated cells on fibronectin, laminin, or Matrigel was decreased significantly.


CONCLUSIONS
Ampelopsin has anti-invasive and anti-metastatic effects on B16 melanoma.
Liu
De-yu
DY
Chemistry Department, Medical Sciences College, Sun Yat-sen University, Guangzhou 510089, Guangdong, China. liudy@gzsums.edu.cn
Zheng
Hong-qiang
HQ
Luo
Gao-qing
GQ
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D019770:Lauraceae / Q000737:chemistry; D008175:Lung Neoplasms / Q000517:prevention & control* / Q000556:secondary; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D010946:Plants, Medicinal / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; C106407:ampelopsin