Immunohistochemical study on the distribution of the voltage-gated potassium channels in the gerbil cerebellum.
Although there have been many studies on the regional distribution of Kv channels in the rat and mouse cerebellum, there are no reports about Kv channel distribution in the gerbil, which is used as an ischemia animal model. Therefore, we aimed to investigate differences in the spatial patterning of Kv channel alpha-subunit isoforms in the gerbil cerebellum. The greatest concentration of Kv1.2 was found in the basket cell axon plexus and terminal regions around the Purkinje cells. Kv1.1 immunoreactivity was also concentrated in this area although the staining intensity was relatively lower. Both Purkinje cell layer and granular layer were intensely stained with anti-Kv1.3 and Kv1.6 antibodies, whereas immunoreactivities for Kv1.4 and Kv1.5 were detected in the Purkinje cell bodies with much lower intensity in the molecular and granular layers. In the cerebellar nuclei, the cell bodies of cerebellar output neurons showed strong immunoreactivities for Kv1.2, Kv1.4, and Kv1.6 with moderate staining for Kv1.3 and Kv1.5 in the cell bodies. This study on the differential localization patterns of Kv1 channel subunits in the gerbil cerebellum may provide helpful guidelines for correlating current types with particular channels and useful data for the future investigations on the pathological conditions such as ischemia and epilepsy.
Chung
Yoon Hee
YH
Department of Anatomy, College of Medicine, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea.
Joo
Kyeung Min
KM
Nam
Ryoung Hee
RH
Kim
Yang Soo
YS
Lee
Won Bok
WB
Cha
Choong Ik
CI
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D005849:Gerbillinae / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000145:classification* / Q000378:metabolism*; D013045:Species Specificity; D014018:Tissue Distribution
D024642:Potassium Channels, Voltage-Gated