title: [Expression of apoptosis related proteins in CD34 positive bone marrow cells of patients with polycythemia vera].
abstract: OBJECTIVE
  To investigate the expression of apoptosis receptor FAS (CD95) and apoptosis related protein Bcl-2 and Bax in CD34 positive bone marrow cells of the patients with polycythemia vera (PV).
  
  
  METHODS
  The expressions of apoptosis receptor FAS (CD95) and apoptosis related protein Bcl-2 and Bax in bone marrow CD34(+) cells from 21 PV patients, 8 essential thrombocythemia (ET) and 11 normal persons were assessed by bicolor flow cytometry (FCM), and the expressions of Bcl-2 and Bax mRNA were assessed by RT-PCR, and their correlation was analysed.
  
  
  RESULTS
  There was no difference between the expressions of CD95 in CD34(+) bone marrow cells of PV patients (42.65 +/- 15.56)%, and that of ET patients (45.31 +/- 17.62)% and of normal person (37.55 +/- 15.19)% (P > 0.05). There was no difference between the expression of Bax in CD34(+) bone marrow cells of PV patients (35.83 +/- 9.33)% and of normal persons (41.65 +/- 9.04)% (P > 0.05). The expression of Bcl-2 in CD34(+) bone marrow cells of PV patients (79.35 +/- 14.43)% was significantly higher than that of normal controls (55.84 +/- 13.43)% (P < 0.01). The ratio of Bax/Bcl-2 of PV patients (0.47 +/- 0.14) was significantly lower than that in normal controls (0.76 +/- 0.24) (P < 0.01). The expression of Bcl-2 mRNA in PV patients' bone marrow hematopoietic cells was higher than that of normal controls (P < 0.01). There was no difference between the expression of Bax mRNA in bone marrow hematopoietic cells of PV patients and that of normal controls. Bcl-2 expression was negatively correlated with Annexin V expression in CD34(+) bone marrow cells of PV patients.
  
  
  CONCLUSIONS
  Over-expression of Bcl-2, one of anti-apoptosis genes, in CD34(+) bone marrow cells might be involved in the lower apoptosis of PV patients.
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Zong-hong
authors.initials: ZH
authors.lastname: Shao
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.forename: Yan-ran
authors.initials: YR
authors.lastname: Cao
authors.forename: Mei-feng
authors.initials: MF
authors.lastname: Tu
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Jia
authors.forename: Juan
authors.initials: J
authors.lastname: Sun
authors.forename: Zhen-zhu
authors.initials: ZZ
authors.lastname: Cui
authors.forename: Lin-sheng
authors.initials: LS
authors.lastname: Qian
authors.forename: Chong-li
authors.initials: CL
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000097:blood*; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011087:Polycythemia Vera / Q000097:blood / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000097:blood / Q000235:genetics; D019014:fas Receptor / Q000097:blood
chemical_list: D018952:Antigens, CD34; C494826:BAX protein, human; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D019014:fas Receptor