title: The protective role of tiopronin in cisplatin ototoxicity in Wistar rats.
abstract: The purpose of this study was to evaluate cisplatin-induced ototoxicity and the protective effects of tiopronin. Twenty-four adult Wistar rats served as subjects and were divided into three groups. Eight rats receiving only saline (group A) were used as controls. Eight rats received cisplatin (2 mg/kg) injections (group B) and eight rats received cisplatin and tiopronin (300 mg/kg) (group C) for 8 consecutive days. Both ears of all animals were tested by DPOAE before treatment and on the 4th and 9th days. Seventy-two hours after the final recording session, all animals were killed, and the left cochleas were prepared for electron microscopy and analysed. DPOAE responses were significantly reduced in group B compared to controls (p<0.05). When tiopronin was added, DPOAE responses were significantly increased compared to those obtained with the administration of cisplatin alone (p<0.05). The cochleogram showed that tiopronin had a significant protective effect in the basal half and in the lower half of the middle turn. We conclude that tiopronin, a drug effective in protecting against cisplatin nephrotoxicity, is also effective in protecting against cisplatin ototoxicity.
authors.affiliation: Institute of Otolaryngology, Catholic University of Rome, Rome, Italy. afetoni@unicatt.it
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Fetoni
authors.forename: Nicola
authors.initials: N
authors.lastname: Quaranta
authors.forename: Raffaella
authors.initials: R
authors.lastname: Marchese
authors.forename: Gabriella
authors.initials: G
authors.lastname: Cadoni
authors.forename: Gaetano
authors.initials: G
authors.lastname: Paludetti
authors.forename: Bruno
authors.initials: B
authors.lastname: Sergi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D016022:Case-Control Studies; D002945:Cisplatin / Q000009:adverse effects*; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000648:ultrastructure; D034381:Hearing Loss / Q000139:chemically induced / Q000517:prevention & control*; D008855:Microscopy, Electron, Scanning; D017084:Otoacoustic Emissions, Spontaneous; D051381:Rats; D017208:Rats, Wistar; D008625:Tiopronin / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D008625:Tiopronin; D002945:Cisplatin