title: Thermoresponsive controlled association of protein with a dynamic nanogel of hydrophobized polysaccharide and cyclodextrin: heat shock protein-like activity of artificial molecular chaperone.
abstract: Dynamic CHP-CD nanogels, which consisted of a self-assembly of cholesteryl-group-bearing pullulan (CHP) and beta-cyclodextrin (CD), were characterized by SEC and SEC-MALS methods. The nanogels prevented the thermal aggregation of carbonic anhydrase B (CAB) by selective trapping of the heat-denatured protein. After the complex between the CHP-CD nanogels and CAB was cooled, the enzyme activity of CAB spontaneously recovered upon release from the complex. The dynamic nanogels self-regulated an association of heat denatured protein and dissociation of native protein depending on the concentration of CD. The thermal stability of CAB was improved by thermoresponsive controlled association between the proteins and the artificial molecular chaperone.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida-Hommachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Nomura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Masaki
authors.initials: M
authors.lastname: Takagi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Narita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Aoyama
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D024401:Carbonic Anhydrase I / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D003505:Cyclodextrins; D005936:Glucans; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D000080385:Nanogels; D011134:Polysaccharides; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; C009109:pullulan; D011094:Polyethyleneimine; D024401:Carbonic Anhydrase I