Assessment of lipid peroxidation and serum vitamin E concentration in dogs with immune-mediated hemolytic anemia.
OBJECTIVE
To determine plasma malondialdehyde (MDA) and serum vitamin E concentrations in dogs with immune-mediated hemolytic anemia (IMHA) and healthy control dogs.


METHODS
Serum and plasma samples from 36 dogs with IMHA and 40 healthy control dogs.


METHODS
Blood samples were collected from all study dogs. Plasma MDA concentrations were measured by use of a commercial colorimetric assay, and serum vitamin E concentrations (alpha-, gamma, and delta-tocopherol concentrations) were measured via high-performance liquid chromatography.


RESULTS
Plasma MDA concentrations were significantly higher in the dogs with IMHA than in the control dogs. Compared with control dogs, serum alpha-, gamma-, and &tocopherol concentrations were significantly lower in the IMHA-affected dogs.


CONCLUSIONS
Results indicated a state of oxidative stress and reduced antioxidant reserve in dogs with IMHA; this finding provides support for further investigation of the potential benefits of antioxidant treatment in dogs with this disease.
Pesillo
S Anna
SA
Department of Clinical Sciences, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.
Freeman
Lisa M
LM
Rush
John E
JE
D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003124:Colorimetry / Q000662:veterinary; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D005260:Female; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D018384:Oxidative Stress; D024505:Tocopherols / Q000097:blood; D014810:Vitamin E / Q000097:blood*
D014810:Vitamin E; D008315:Malondialdehyde; D024505:Tocopherols