title: Viral expression and leukocyte adhesion after in vitro infection of goat mammary gland cells with caprine arthritis-encephalitis virus.
abstract: A characteristic lesion in goats infected by the lentivirus CAEV is mastitis with lymphoid hyperplasia. In order to investigate the mechanism of lesion formation, cultures highly enriched in microvascular endothelial cells, mature and immature luminal epithelial cells, fibroblasts and myoepithelial cells were established from goat mammary gland biopsies. Their susceptibility to in vitro infection with two distinct types of CAEV was investigated by PCR, antigen expression and cytopathy. The capacity of infected mammary gland cells to bind uninfected caprine leukocytes was determined by flow cytometry. All cell types tested were susceptible to CAEV infection in vitro, with different levels of sensitivity according to cell phenotype. Our results suggest that the limited extent of natural infection of mammary gland cells reflects a protective local immune response, and that the myoepithelial cell could act as a reservoir cell. After infection, the mature luminal cell acquires the capacity to bind leukocytes in vitro, which could indicate a facilitation of cellular interactions. The distinct reactions of the different cell types to CAEV infection may be correlated with events leading to progressive lesion development during the natural infection.
authors.affiliation: UMR 754, Rétrovirus et Pathologie Comparée, INRA/ENVL/UCBL/EPHE, Université Claude Bernard, IFR 128 Biosciences Lyon Gerland, 50 Avenue Tony Garnier, 69366 Lyon Cedex 07, France.
authors.forename: N
authors.initials: N
authors.lastname: Milhau
authors.forename: P
authors.initials: P
authors.lastname: Renson
authors.forename: I
authors.initials: I
authors.lastname: Dreesen
authors.forename: T
authors.initials: T
authors.lastname: Greenland
authors.forename: C
authors.initials: C
authors.lastname: Bellaton
authors.forename: F
authors.initials: F
authors.lastname: Guiguen
authors.forename: J F
authors.initials: JF
authors.lastname: Mornex
authors.forename: C
authors.initials: C
authors.lastname: Le Jan
mesh_terms: D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000302:isolation & purification / Q000472:pathogenicity*; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D006041:Goats; D007962:Leukocytes / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification