Neuronal activity-induced regulation of Lingo-1.
Axonal regeneration after injury can be limited in the adult CNS by the presence of inhibitory proteins such as Nogo. Nogo binds to a receptor complex that consists of Nogo receptor (NgR), p75NTR, and Lingo-1. Nogo binding activates RhoA, which inhibits axonal outgrowth. Here we assessed Lingo-1 and NgR mRNA levels after delivery of BDNF into the rat hippocampal formation, Lingo-1 mRNA levels in rats subjected to kainic acid (KA) and running in running wheels. Lingo-1 mRNA was not changed by running. However, we found that Lingo-1 mRNA was strongly up-regulated while NgR mRNA was down-regulated in the dentate gyrus in both the BDNF and the KA experiments. Our data demonstrate inverse regulation of NgR and Lingo-1 in these situations, suggesting that Lingo-1 up-regulation is one characteristic of activity-induced neural plasticity responses.
Trifunovski
Alexandra
A
Department of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden. Alexandra.Trifunovski@neuro.ki.se
Josephson
Anna
A
Ringman
Andreas
A
Brené
Stefan
S
Spenger
Christian
C
Olson
Lars
L
D000818:Animals; D001522:Behavior, Animal; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D058851:GPI-Linked Proteins; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000293:injuries; D007091:Image Processing, Computer-Assisted / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D007608:Kainic Acid / Q000633:toxicity; D008565:Membrane Proteins; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism*; D000070816:Nogo Receptor 1; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide / Q000235:genetics / Q000378:metabolism*; D012420:Running / Q000502:physiology; D013997:Time Factors
D019208:Brain-Derived Neurotrophic Factor; D018690:Excitatory Amino Acid Agonists; D058851:GPI-Linked Proteins; C482293:LINGO1 protein, human; D008565:Membrane Proteins; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D009419:Nerve Tissue Proteins; D000070816:Nogo Receptor 1; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; C486581:Rtn4r protein, rat; D007608:Kainic Acid