title: Differential regulation of metallothionein-I and metallothionein-II mRNA expression in the rat brain following traumatic brain injury.
abstract: In this study, we investigated the expression of metallothionein (MT)-I and MT-II in the rat brain following traumatic brain injury (TBI). In the early stage, significant induction of MT-I and MT-II were observed in various regions including ventricle walls, pia mater, and dentate gyrus. At 12-24 h after TBI, strong induction of MT-I mRNA was observed in cerebral cortical layer II/III, amygdala, and piriform cortex where neurons reside. On the other hand, MT-II appeared to be expressed mainly in glial cells localized in the cerebral cortex and hippocampal formation. Three days after TBI, MTs were observed in the vimentin-positive astrocytes in the penumbra as revealed by double immunohistochemistry. The differences in expression of MT-I and MT-II in different brain regions and cell types (neuron vs. glial cells) suggests that multiple regulatory mechanisms are involved in the control of MT expression following brain injury.
authors.affiliation: Department of Anatomy, College of Medicine, Brain Korea 21, Korea University, Seoul 136-705, Korea.
authors.forename: Eun Hae
authors.initials: EH
authors.lastname: Kim
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Kim
authors.forename: Woong
authors.initials: W
authors.lastname: Sun
authors.forename: Dal Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Chung
authors.forename: Dong Ki
authors.initials: DK
authors.lastname: Kim
authors.forename: Sun-Hwa
authors.initials: SH
authors.lastname: Park
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D008668:Metallothionein