title: [Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria].
abstract: OBJECTIVE
  To detect the expression of glycosyl-phosphatidyl-inositol (GPI) anchored protein on the blood cell membrane and its implication in the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH).
  
  
  METHODS
  The GPI anchored protein was detected by indirect immunofluorescence method with a panel of monoclonal antibodies (CD55, CD59, CD16, CD58, HIR2, CD15, CD2) in 22 PNH patients.
  
  
  RESULTS
  The abnormal granulocytes and erythrocytes with deficient GPI anchored proteins were identified in all PNH patients whether the routine hemolysis tests for PNH, including Ham test and mCLST, were positive or negative. However, lymphocytes with PNH-defect were found in only 14 patients.
  
  
  CONCLUSIONS
  GPI anchored protein assay provided sensitive and specific method for the diagnosis of PNH.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Shao
authors.forename: H
authors.initials: H
authors.lastname: Jia
authors.forename: Y
authors.initials: Y
authors.lastname: Chu
authors.forename: D
authors.initials: D
authors.lastname: Shen
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000145:classification* / Q000276:immunology* / Q000378:metabolism; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000378:metabolism; D004912:Erythrocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D006098:Granulocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D008214:Lymphocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism; D008297:Male
chemical_list: D018797:Cell Cycle Proteins