Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 (OsNDPK1) in rice plants upon infection with bacterial pathogens.
A cDNA library was constructed using mRNA extracted from rice leaves infected with Xanthomonas oryzae pv. oryzae (Xoo), a bacterial leaf blight pathogen, to isolate rice genes induced by Xoo infection. Subtractive hybridization and differential screening of the cDNA library led to the isolation of many induced genes including a nucleotide diphosphate kinase 1 (OsNDPK1) and a pathogenesis-related protein 1 (OsPR1) cDNA. Nucleoside diphosphate kinases (NDPKs) are key metabolic enzymes that maintain the balance between cellular ATP and other nucleoside triphosphates (NTPs). Three other OsNDPK genes (NP922751, OsNDPK2 and OsNDPK3) found in databases were obtained by RT-PCR. Three different programs for predicting subcellular targeting indicated that OsNDPK1 and NP922751 were non-organellar, OsNDPK2 plastidic, and OsNDPK3 mitochondrial. Only transcripts of OsNDPK1 accumulated strongly after infection with Xoo. When rice plants were infected with Burkholderia glumae, a bacterial grain/seedling rot pathogen, the pattern of expression of the rice NDPK genes was similar to that following infection with Xoo. OsNDPK1 gene expression was also strongly induced in response to exposure to salicylic acid, jasmonic acid, and abscisic acid, although the level of transcripts and their pattern of expression depended on the inducer.
Cho
Song Mi
SM
Agricultural Plant Stress Research Center, Applied Plant Science Division, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea.
Shin
Seo Ho
SH
Kim
Kwang Sang
KS
Kim
Young Cheol
YC
Eun
Moo Young
MY
Cho
Baik Ho
BH
D000040:Abscisic Acid / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D019117:Burkholderia / Q000235:genetics; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D009693:Nucleic Acid Hybridization; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis* / Q000235:genetics*; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D054883:Oxylipins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D014974:Xanthomonas / Q000378:metabolism
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; C011006:jasmonic acid; D000040:Abscisic Acid; D000255:Adenosine Triphosphate; D009701:Nucleoside-Diphosphate Kinase; D020156:Salicylic Acid