title: Determination of dipyridamole in pharmaceutical preparations using square wave voltammetry.
abstract: An analytical methodology using square wave voltammetry (SWV) at a hanging mercury drop electrode (HMDE) was developed for the quantitative determination of dipyridamole (DIP), a drug used for the treatment of several cardiovascular diseases, in pharmaceutical tablets and injections of Persantin in phosphate buffer (pH 3.0; 0.1M). After optimization of the parameters for SWV, analytical curves were obtained for application in the range of 1.28 x 10(-6)M to 7.02 x 10(-6)M. It was found a detection limit (DL) of 1.88 x 10(-8)M (9.50 ng/ml). The repeatability and the reproducibility of the method were determinated by successive measurements of DIP solutions on the range of the analytical curve with a coefficient variation of 0.97% (n=5) and 1.15%, respectively. The apparent recoveries were obtained by the IUPAC recommended procedure using the second reduction peak. Recoveries obtained by SWV were compared with the UV-vis spectrophotometric method. It was found that the determination of DIP in Persantin tablets gave a mean value of 75.6+/-0.4 mg (100.8%) and 68.9+/-0.3 mg (91.8%) for SWV and UV-vis spectrophotometry, respectively. In the case of injections, it was found 10.4+/-0.1 mg (103.4%) and 9.9+/-0.2 mg (99.9%) for SWV and UV-vis spectrophotometry. Both apparent recoveries for the two types of formulations are in good accordance with the declared value of 75 mg (tablets) and 10 mg (injections).
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, Departmentamento de Fisico-Quimica, Avenida Trabalhador Sãocarlense, CP 780, 13560-970 São Carlos SP, Brazil.
authors.forename: Renata Alves
authors.initials: RA
authors.lastname: de Toledo
authors.forename: Marilza
authors.initials: M
authors.lastname: Castilho
authors.forename: Luiz Henrique
authors.initials: LH
authors.lastname: Mazo
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004176:Dipyridamole / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D004364:Pharmaceutical Preparations; D004176:Dipyridamole