Efficacy of topotecan treatment on antioxidant enzymes and TBA-RS levels in submandibular glands of rabbits: an experimental study.
OBJECTIVE
The aim of this study was to investigate the effects of topotecan (Hycamtin), a topoisomerase I inhibiting anticancer agent, on antioxidant enzymes (SOD, CAT, and GSH-Px) and TBA-RS values of the submandibular glands of the rabbits.


METHODS
The study was conveyed in two groups (Group I, II) and control with a total of 24 rabbits. Eight rabbits in group I received intravenous (i.v.) topotecan (0.25 mg/kg once daily) for 3 days. Eight rabbits in group II received i.v. topotecan (0.5 mg/kg once daily) for 3 days. On the 15th day after administration of topotecan, submandibular glands were removed and levels of the SOD, CAT, and GSH-Px and the TBA-RS in the submandibular glands of the rabbits were examined.


RESULTS
SOD, CAT, and GSH-Px values were significantly higher in high-dose topotecan group compared to control group (P < 0.05). SOD and TBA-RS values were significantly higher in high-dose topotecan group compared to low-dose topotecan group (P < 0.05).


CONCLUSIONS
It was concluded that, to prevent the hazardous effects of oxygen free radicals due to topotecan, antioxidant enzymes SOD, CAT, and GSH-Px were increased. The higher levels of the TBA-RS values in group II showed that permanent damage was present because of high-dose topotecan administration in the submandibular glands of the rabbits.
Muluk
Nuray Bayar
NB
ENT Department, Kirikkale University, Faculty of Medicine, Ankara, Turkey. nurayb@hotmail.com
Kisa
Uçler
U
Kaçmaz
Murat
M
Apan
Alpaslan
A
Koç
Can
C
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002374:Catalase / Q000032:analysis*; D005979:Glutathione Peroxidase / Q000032:analysis*; D008297:Male; D011817:Rabbits; D013363:Submandibular Gland / Q000737:chemistry* / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000032:analysis*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*; D019772:Topotecan / Q000494:pharmacology*
D000970:Antineoplastic Agents; D017392:Thiobarbituric Acid Reactive Substances; D019772:Topotecan; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase