title: Radio frequency plasma polymer coatings for affinity capture MALDI mass spectrometry.
abstract: Surface modification of MALDI probes is an attractive approach for combining bioaffinity isolation of targeted biomolecules with mass spectrometric analysis of the captured species. In this work, we demonstrate that a polymer thin film, produced by pulsed rf plasma polymerization of allylamine and deposited directly on a MALDI probe, can be subsequently biotinylated to develop a bioaffinity capture MALDI probe. The synthesis and characterization of the probe by XPS, FT-IR, and AFM is described, and the selective isolation of avidin from a three-component mixture of avidin, lysozyme, and cytochrome c is presented. These initial results offer encouragement for the further exploration of rf plasma polymer deposition as a novel approach for the development of on-probe affinity capture MALDI probes.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019-0065, USA.
authors.forename: Meiling
authors.initials: M
authors.lastname: Li
authors.forename: Richard B
authors.initials: RB
authors.lastname: Timmons
authors.forename: Gary R
authors.initials: GR
authors.lastname: Kinsel
mesh_terms: D019921:Biotinylation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D011846:Radio Waves; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D011506:Proteins