[Protein splicing and its application in protein engineering].
Protein splicing, which is an intein mediated posttranslational processing, involves a series of intramolecular cleavage-ligation reactions. Intein is an intervening polypeptide which can catalytic self-cleavage from a pre-protein accompanied by the concomitant joining of the two flanking polypeptides (the extein) through a peptide bond. Protein splicing not only enriches genetic theory of posttranslational processing, but also have wide application prospect.
Hao
Dong-Mei
DM
College of Biology, Zhejiang University, Hangzhou 310027, China. dmh11617@sohu.com
Qian
Kai-Xian
KX
Shen
Gui-Fang
GF
D047669:Exteins / Q000235:genetics; D047668:Inteins / Q000235:genetics; D015202:Protein Engineering; D019154:Protein Splicing / Q000235:genetics; D012326:RNA Splicing