title: Red wine polyphenolics suppress the secretion and the synthesis of Apo B48 from human intestinal CaCo-2 cells.
abstract: Epidemiological studies suggest that the red wine consumption may reduce the risk factor of cardiovascular disease. However, the mechanisms of how the red wine phenolic components reduce the risk of cardiovascular disease is currently unknown. Our previous study demonstrated that red wine polyphenolics suppress the secretion of pro-atherogenic lipoproteins (very low density lipoproteins) from human hepatic HepG2 cells. Therefore, in this study we hypothesize that red wine polyphenolics will also attenuate the production and secretion of another pro-atherogenic lipoprotein (chylomicrons) from human intestinal CaCo-2 cells. Cultured CaCo-2 cells were incubated in the presence of dealcoholized red wine, alcoholized red wine and atorvastatin for 24 h. The apo B48 protein (marker of intestinal chylomicrons) was quantified on Western blotting and the enhanced chemiluminescence. Apo B48 levels in the cells and that secreted into the media were significantly reduced by 29% in the cells incubated with dealcoholized red wine compared with control cells. Also the similar effect was shown in the cells incubated with alcoholized red wine. The cells incubated with atorvastatin shown the significant reduction of apo B48 production compared to control cells. Collectively, this study suggests that red wine polyphenolics down regulate the production of chylomicron in intestinal CaCo-2 cells.
authors.affiliation: School of Humanities, Environmental Policy and Technology, Himeji Institute of Technology, Japan.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Takechi
authors.forename: Naoko
authors.initials: N
authors.lastname: Hiramatsu
authors.forename: John C L
authors.initials: JC
authors.lastname: Mamo
authors.forename: Sebely
authors.initials: S
authors.lastname: Pal
mesh_terms: D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D000069059:Atorvastatin; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006538:Heptanoic Acids / Q000494:pharmacology; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D011758:Pyrroles / Q000494:pharmacology; D014920:Wine / Q000032:analysis*
chemical_list: D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D005419:Flavonoids; D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D010636:Phenols; D059808:Polyphenols; D011758:Pyrroles; D000069059:Atorvastatin