[Susceptibility of 15 collections of Aedes albopictus from Guizhou to dengue virus oral infection].
OBJECTIVE
To study the susceptibility of Aedes albopictus to dengue virus infection.


METHODS
Aedes albopictus from 15 collections in Guizhou province were challenged orally with dengue virus 1-4 types, respectively. The total RNA from mosquitos were extracted. The viral NS1 gene fragment was amplified with reverse transcriptase polymerase chain reaction (RT-PCR). Dengue virus in mosquitoes was isolated with C6/36 cells. Then the viral antigen was detected by indirect immunofluorescence assay (IFA). Antigen and nucleic acid of dengue virus from 15 geographic strains of Aedes albopictus orally infected with dengue viruses (DEN-1, DEN-2, DEN-3 and DEN-4) were detected by IFA, RT-PCR and the virus was isolated with C6/36 cells, respectively.


RESULTS
The rates of Aedes albopictus orally infected with DEN-1, DEN-2, DEN-3 and DEN-4 were 12/15, 12/15, 8/15 and 13/15, respectively.


CONCLUSIONS
Different geographic strains of Aedes albopitus in Guizhou were susceptible to dengue viruses.
Shu
Li-ping
LP
Department of Immunology, Guiyang Medical College, Guiyang 550004, China.
Zuo
Li
L
Zhao
Xing
X
Chen
A-ying
AY
Wei
Long-hua
LH
D000330:Aedes / Q000166:cytology / Q000821:virology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002681:China; D004279:DNA, Viral / Q000032:analysis*; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D004198:Disease Susceptibility; D017753:Ecosystem; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017361:Viral Nonstructural Proteins / Q000235:genetics
D000956:Antigens, Viral; D004279:DNA, Viral; C082515:NS1 protein, dengue-1 virus; D017361:Viral Nonstructural Proteins