title: Immune reconstitution after allogeneic stem cell transplantation: the impact of stem cell source and graft-versus-host disease.
abstract: OBJECTIVE
  Bone marrow (BM) and blood stem cell (BSC) allografts differ considerably with respect to their content of progenitor cells and progenitor cell subsets as well as mature lymphocytes. The aim of this prospective, randomized study was to determine whether these differences have an impact on early post-transplant immune recovery.
  
  
  METHODS
  In a prospective randomised study, we found enhanced immune recovery in recipients of BSC allografts compared to in recipients of BM allografts despite transplantation of a lower number of lymphoid progenitors, particularly B-cell progenitors. The large number of mature lymphocytes in BSC allografts is a plausible explanation for this observation. At the progenitor cell level, we found a comparable and very high proportion of progenitor cells involved in lymphopoiesis in both study groups.
  
  
  RESULTS
  Patients with extensive chronic GVHD, irrespective of the allograft received, had low immunoglobulin (Ig) levels in serum, low B-cell counts in blood and low numbers of B-cell progenitors in the bone marrow. They also showed high T-cell counts, particularly CD3+CD8+ T-cell counts, which was paralleled by a high number of T-cell progenitors in the bone marrow. In patients with extensive chronic GVHD we found low natural killer (NK)-cell counts which has not been reported previously.
  
  
  CONCLUSIONS
  Early immune recovery is enhanced following BSC allografting compared with BM allografting. This is plausibly explained by the large inoculum of mature lymphocytes in BSC allografts. Following allografting, a higher proportion of the BM progenitor cell compartment is involved in lymphopoiesis than it is in healthy adults. However, B-lymphopoiesis is inhibited in patients with extensive chronic GVHD resulting in impaired B-cell recovery. These patients also seem to show impaired NK-cell recovery.
authors.affiliation: Medical Department, Rikshosptialet University Hospital N-0027 Oslo, Norway.
authors.forename: Ingerid Weum
authors.initials: IW
authors.lastname: Abrahamsen
authors.forename: Stig
authors.initials: S
authors.lastname: Sømme
authors.forename: Dag
authors.initials: D
authors.lastname: Heldal
authors.forename: Torstein
authors.initials: T
authors.lastname: Egeland
authors.forename: Dag
authors.initials: D
authors.lastname: Kvale
authors.forename: Geir Erland
authors.initials: GE
authors.lastname: Tjønnfjord
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000097:blood; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000276:immunology*; D015331:Cohort Studies; D005260:Female; D006086:Graft vs Host Disease / Q000145:classification / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D014184:Transplantation, Homologous / Q000276:immunology / Q000401:mortality
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D007136:Immunoglobulins