Oxidation of titanium, RGD peptide attachment, and matrix mineralization rat bone marrow stromal cells.
The aim of this study was to compare the efficacy of attachment of arginine-glycine-aspartic acid (RGD) peptide to titanium surfaces oxidized by different methods. Titanium surfaces were treated as follows: (1) treatment A: passivation in nitric acid, (2) treatment B: heated in air at 400 degrees C for 1 hour, (3) treatment C: immersed in 8.8 M H2O2/0.1 M HCl at 80 degrees C for 30 minutes, and (4) treatment D: treated as in treatment C and then heated at 400 degrees C for 1 hour. RGD was attached to titanium samples treated as in treatments A through D. The quantity of attached RGD was determined by an enzyme-linked immunoabsorbent assay. Mineralization of a rat bone marrow stromal cell (RMSC) culture on the titanium surfaces after 21 days was determined y atomic absorption spectroscopy. The treatments were ranked according to quantity of RGD attached as C, A, B, and D. Twenty-one days after RMSC culture, the degree of mineralization was significantly higher for treatment C than for treatments A, B, and D and for controls. The efficacy of RGD attachment varies with the oxidation treatment given to titanium. Oxidation in H2O2/0.1 M HCl at 80 degrees C provided the best overall surface for RGD attachment as well as calcified matrix formation of RMSCs.
Mante
Francis K
FK
University of Pennsylvania School of Dental Medicine, 4001 Spruce Street, Philadelphia, PA 19104, USA. mantefk@pobox.upenn.edu
Little
Kevin
K
Mante
Mamle O
MO
Rawle
Christopher
C
Baran
George R
GR
D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology*; D002118:Calcium / Q000032:analysis; D005260:Female; D006358:Hot Temperature; D006851:Hydrochloric Acid / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D039302:Integrin alphaVbeta3 / Q000032:analysis; D009569:Nitric Oxide / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000502:physiology; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D051381:Rats; D011920:Rats, Inbred WF; D017154:Stromal Cells / Q000502:physiology*; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D016877:Oxidants; C009495:titanium dioxide; D009569:Nitric Oxide; C047981:arginyl-glycyl-aspartic acid; D006861:Hydrogen Peroxide; D014025:Titanium; D006851:Hydrochloric Acid; D002118:Calcium