Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling.
We describe a detailed study on the possibility of analyzing aminated pesticides and metabolites using pre-column derivatization with fluorescein isothiocyanate (FITC) and their subsequent separation and detection by cyclodextrin capillary electrophoresis-laser-induced fluorescence (CE-LIF) detection. Different variables affecting the derivatization reaction (pH, FITC concentration, reaction time and temperature) and those related with the separation itself (buffer concentration, addition of various organic modifiers, pH, applied voltage and injection time) were studied. The limit of detection obtained was between 0.45 and 3.48 microg litre(-1) showing a relative standard deviation between 0.26 and 2.08% at a concentration level of 50 microg litre(-1).
Navarrete-Casas
Miguel
M
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva s/n, E-18071 Granada, Spain.
Segura-Carretero
Antonio
A
Cruces-Blanco
Carmen
C
Fernández-Gutiérrez
Alberto
A
D000588:Amines / Q000032:analysis* / Q000737:chemistry; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D015394:Molecular Structure; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis
D000588:Amines; D003505:Cyclodextrins; D005456:Fluorescent Dyes; D010575:Pesticides; D014874:Water Pollutants, Chemical; D016650:Fluorescein-5-isothiocyanate