title: Assessment of lipid peroxidation and serum vitamin E concentration in dogs with immune-mediated hemolytic anemia.
abstract: OBJECTIVE
  To determine plasma malondialdehyde (MDA) and serum vitamin E concentrations in dogs with immune-mediated hemolytic anemia (IMHA) and healthy control dogs.
  
  
  METHODS
  Serum and plasma samples from 36 dogs with IMHA and 40 healthy control dogs.
  
  
  METHODS
  Blood samples were collected from all study dogs. Plasma MDA concentrations were measured by use of a commercial colorimetric assay, and serum vitamin E concentrations (alpha-, gamma, and delta-tocopherol concentrations) were measured via high-performance liquid chromatography.
  
  
  RESULTS
  Plasma MDA concentrations were significantly higher in the dogs with IMHA than in the control dogs. Compared with control dogs, serum alpha-, gamma-, and &tocopherol concentrations were significantly lower in the IMHA-affected dogs.
  
  
  CONCLUSIONS
  Results indicated a state of oxidative stress and reduced antioxidant reserve in dogs with IMHA; this finding provides support for further investigation of the potential benefits of antioxidant treatment in dogs with this disease.
authors.affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.
authors.forename: S Anna
authors.initials: SA
authors.lastname: Pesillo
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Freeman
authors.forename: John E
authors.initials: JE
authors.lastname: Rush
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003124:Colorimetry / Q000662:veterinary; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D005260:Female; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D018384:Oxidative Stress; D024505:Tocopherols / Q000097:blood; D014810:Vitamin E / Q000097:blood*
chemical_list: D014810:Vitamin E; D008315:Malondialdehyde; D024505:Tocopherols