title: Isolation of multipotent cells from human term placenta.
abstract: Current sources of stem cells include embryonic stem cells (ESCs) and adult stem cells (ASCs). However, concerns exist with either source: ESCs, with their significant ethical considerations, tumorigenicity, and paucity of cell lines; and ASCs, which are possibly more limited in potential. Thus, the search continues for an ethically conducive, easily accessible, and high-yielding source of stem cells. We have isolated a population of multipotent cells from the human term placenta, a temporary organ with fetal contributions that is discarded postpartum. These placenta-derived multipotent cells (PDMCs) exhibit many markers common to mesenchymal stem cells--including CD105/endoglin/SH-2, SH-3, and SH-4--and they lack hematopoietic-, endothelial-, and trophoblastic-specific cell markers. In addition, PDMCs exhibit ESC surface markers of SSEA-4, TRA-1-61, and TRA-1-80. Adipogenic, osteogenic, and neurogenic differentiation were achieved after culturing under the appropriate conditions. PDMCs could provide an ethically uncontroversial and easily accessible source of multipotent cells for future experimental and clinical applications.
authors.affiliation: Stem Cell Research Center, National Health Research Institutes, Min-Chuan E. Road, Taipei, 114, Taiwan.
authors.forename: B Linju
authors.initials: BL
authors.lastname: Yen
authors.forename: Hsing-I
authors.initials: HI
authors.lastname: Huang
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chien
authors.forename: Hsiang-Yiang
authors.initials: HY
authors.lastname: Jui
authors.forename: Bor-Sheng
authors.initials: BS
authors.lastname: Ko
authors.forename: Ming
authors.initials: M
authors.lastname: Yao
authors.forename: Chia-Tung
authors.initials: CT
authors.lastname: Shun
authors.forename: Men-Luh
authors.initials: ML
authors.lastname: Yen
authors.forename: Meng-Chou
authors.initials: MC
authors.lastname: Lee
authors.forename: Yao-Chang
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D039902:Multipotent Stem Cells / Q000166:cytology*; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D012016:Reference Values; D047929:Term Birth
chemical_list: D018997:Chorionic Gonadotropin, beta Subunit, Human