title: [HLA-DR-positive T lymphocytes in the bone marrow and peripheral blood of patients with severe aplastic anemia and their in vitro inhibitory effecton BFU-E and CFU-GM].
abstract: OBJECTIVE
  [corrected] To elucidate the role of immunodysfunction in the pathogenesis of severe aplastic anemia (SAA).
  
  
  METHODS
  HLA-DR+ T cells were detected in bone marrow (BM) and peripheral blood (PB) of 20 SAA patients and controls (9 for BM and 11 for PB). PHA-LYCM conditioned medium was prepared, and its in vitro effect on normal bone marrow BFU-E and CFU-GM was studied.
  
  
  RESULTS
  The percentages of HLA-DR+ T lymphocytes in BM and PB of newly diagnosed SAA patients were significantly higher than that of controls (P<0.001), and that of recovered SAA (rSAA) patients after ALG therapy (P<0.05). Compared with controls, the PBA-LYCMs from newly diagnosed SAA patients showed significant inhibitory effect on normal BFU-E and CFU-GM (P<0.001).
  
  
  CONCLUSIONS
  The activated T lymphocyte may play an important role in the pathogenesis of SAA.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
authors.forename: C
authors.initials: C
authors.lastname: Xia
authors.forename: Y
authors.initials: Y
authors.lastname: Chu
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Shao
authors.forename: Z
authors.initials: Z
authors.lastname: Tian
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Tang
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000188:drug therapy / Q000276:immunology* / Q000628:therapy; D000961:Antilymphocyte Serum / Q000627:therapeutic use; D016022:Case-Control Studies; D002648:Child; D015672:Erythroid Precursor Cells / Q000276:immunology*; D055014:Granulocyte-Macrophage Progenitor Cells / Q000276:immunology*; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D000961:Antilymphocyte Serum; D006684:HLA-DR Antigens