title: [Detection of minimal residual disease after allogeneic bone marrow transplantation in chronic myeloid leukemia].
abstract: OBJECTIVE
  To detect minimal residual disease (MRD) after allogeneic bone marrow transplantation (allo-BMT) in chronic myeloid leukemia (CML).
  
  
  METHODS
  M-bcr/abl mRNA was assayed by reverse transcriptase polymerase chain reaction (RT-PCR) in bone marrow cells from 46 successfully sibling marrow engrafted CML patients.
  
  
  RESULTS
  About 70% of the patients achieved genetic complete remission in 3 months post allo-BMT. Four patients were M-bcr/abc mRNA positive at 1.5 to 2 months post allo-BMT and turned to be negative at 3 to 9 months post allo-BMT. One patient was still M-bcr/abl(+) after disease-free survival(DFS) for more than 6 years, while another one was M-bcr/abl(-) after DFS for more than 4 years.
  
  
  CONCLUSIONS
  RT-PCR is so far the most sensitive method for MRD detection in CML, but its limitation should not be ignored.
authors.affiliation: Institute of Hematology, Beijing Medical University, Beijing 100034.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Fu
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: Y
authors.initials: Y
authors.lastname: Chang
authors.forename: W
authors.initials: W
authors.lastname: Xue
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000601:surgery*; D008297:Male; D018365:Neoplasm, Residual; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014184:Transplantation, Homologous; D055815:Young Adult