title: [Molecular mutations of rpoB gene of multidrug resistant Mycobacterium tuberculosis isolates from China].
abstract: To characterize rpoB mutations of multidrug resistant Mycobacterium tuberculosis clinical isolates from China, mutations in the 81 bp rifampin resistance determining region (RRDR) and mutation V176F locating in the beginning of the rpoB gene were analyzed by DNA sequencing. Eighty six Mycobacterium tuberculosis clinical isolates, including 72 rifampin resistant strains and 14 rifampin sensitive strains were sequenced. Six five mutations of 22 distinct kinds, 21 point mutations and one insertion, were observed in 65 of 72 resistant isolates. The most frequent mutations were in codon 531 (41%), 526 (40%), and 516 (4%). Mutations were not found in seven (10%) of the resistant isolates. Six new alleles within the RRDR, along with five novel mutations outside the RRDR, are reported. None of isolates contained the V176 mutation.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, College of Life Science, Fudan University, Shanghai 200433, China. yuejunnan@yahoo.com.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Yue
authors.forename: Er-Liang
authors.initials: EL
authors.lastname: Zeng
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xie
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Hong-Hai
authors.initials: HH
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*
chemical_list: C119094:ARR-2 protein, Bacteria; D001426:Bacterial Proteins; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit