title: Simple and rapid determination of 1-deoxynojirimycin in mulberry leaves.
abstract: A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inhibitor present in mulberry leaves (Morus alba and Morus bombysis), by high performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from extract of mulberry leaves on TSK gel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During post column detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.
authors.affiliation: National Agricultural Research Center for Tohoku Region, Fukushima 960-2156, Japan. kmr@affrc.go.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kimura
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Saito
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamagishi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Suzuki
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis*; D000998:Antiviral Agents / Q000032:analysis; D001628:Beverages; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004791:Enzyme Inhibitors / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D013607:Tablets
chemical_list: D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D013607:Tablets; D017485:1-Deoxynojirimycin