Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.
RNA polymerase II (pol II) purified from the fission yeast Schizosaccharomyces pombe was previously reported to be associated with the general transcription factor TFIIF and the C-terminal domain phosphatase Fcp1, as well as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which has recently been implicated in transcriptional activation in human cells. Here, we provide evidence that the Rpb7 subunit of pol II interacts with GAPDH. Two-hybrid screen identified GAPDH as an Rpb7-binding protein. In addition, GAPDH was affinity-purified from S. pombe extract by using an Rpb4/Rpb7-coupled column. We also identified actin as a pol II-associated protein and revealed the interaction between actin and Rpb7.
Mitsuzawa
Hiroshi
H
Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. hmitsuza@lab.nig.ac.jp
Kimura
Makoto
M
Kanda
Emi
E
Ishihama
Akira
A
D000199:Actins / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D000199:Actins; D029702:Schizosaccharomyces pombe Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D012319:RNA Polymerase II; C496332:Rpb7 protein, S pombe