Clinical usefulness of K-RAS mutation detection in colorectal cancer and in surgical margins of the colon.
The incidence of K-RAS gene mutations in tumour and surgical margins was investigated in 63 patients with adenocarcinomas of varied clinical stage and histological grade. Point mutations of codon 12 K-RAS gene were detected, using the PCR-RFPL technique in cancer tissue in 23 patients (36.5%) and in colon margin mucosa in 1 patient (3.7%), out of 27 examined subjects. No significant correlations were found between the mutations and clinical features. Tumours, located in the left colon, and mucinous neoplasms displayed a higher incidence of mutations. No correlation was observed with either Dukes or TMN clinical advancement.
Okulczyk
B
B
The 2nd Department of General and Gastroenterological Surgery, Medical University of Białystok, Poland.
Kovalchuk
O
O
Piotrowski
Z
Z
Myśliwiec
P
P
Chyczewski
L
L
D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000601:surgery; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000601:surgery; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004273:DNA, Neoplasm