[The anti-leukemia effects of IL-2 and IL-3 genes co-transfected leukemia vaccine].
OBJECTIVE
To investigate the anti-leukemia activity of IL-2 and IL-3 genes co-transfected leukemia vaccine.


METHODS
The vaccine was prepared with FBL-3 erythroleukemia cells which were in vitro transfected with IL-2 and/or IL-3 genes by recombinant adenovirus and then inactivated by 60Co irradiation. The vaccine was injected peritoneally into FBL-3 leukemia mice, and the tumor growth, survival time of the mice, cytotoxicities of the peritoneal macrophages, splenic NK cells and CTL cells were observed.


RESULTS
A fter treatment with the vaccine, the leukemic cell tumor in the mice showed significant growth inhibition and the survival time of the mice were prolonged when treated with IL-2 transfected vaccine, the cytotoxicities of splenic NK and CTL were increased significantly, while in the case of IL-3 transfected vaccine, the number and cytotoxicity of peritoneal macrophages were increased. The antileukemia effect was augmented when treated with IL-2 and IL-3 genes co-transfected vaccine. The antileukemia activity of the vaccine was also augmented by low dose cyclophosphamide administration.


CONCLUSIONS
Leukemia vaccine gained more antitumor effect via induction of antitumor immunity.
Yu
M
M
Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433.
Yang
J
J
Cao
X
X
D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D003520:Cyclophosphamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007376:Interleukin-2 / Q000235:genetics*; D007377:Interleukin-3 / Q000235:genetics*; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D007938:Leukemia / Q000235:genetics / Q000276:immunology* / Q000628:therapy*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015996:Survival Rate; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D014162:Transfection
D019496:Cancer Vaccines; D007376:Interleukin-2; D007377:Interleukin-3; D003520:Cyclophosphamide