[Effects of oxymatrine on serum levels of Th1/Th2 type cytokines in HBsAg transgenic mice].
OBJECTIVE
To explore the effects of oxymatrine on serum levels of Th1/Th2 cytokines in HBsAg transgenic mice.


METHODS
HBsAg transgenic mice were divided into oxymatrine group and control group. Each mouse was injected with either oxymatrine 200 mg/kg 0.2 ml or 0.9% NaCl 0.2 ml intraperitoneally once a day for 30 days. Serum IFN-gamma, IL-2 and IL-4, IL-10 were quantitated before and after different treatment.


RESULTS
There was no significant difference on the levels of IFN-gamma and IL-4 before and after treatment in control group. While in oxymatrine group, the levels of IFN-gamma before and after treatment were (3.108+/-3.172) pg/ml and (11.059+/-6.971) pg/ml; those of IL-4 were (29.045+/-13.235) pg/ml and (13.024+/-9.002) pg/ml (both P less than 0.001). After treatment, the levels of IL-2 in control and oxymatrine group were (1.070+/-0.447) pg/ml and (5.537+/-2.887) pg/ml (P less than 0.000 1); and those of IL-10 were (97.226+/-73.306) pg/ml and (33.607+/-23.154) pg/ml (P less than 0.01).


CONCLUSIONS
After injection of oxymatrine to HBsAg transgenic mice, the serum concentration of Th1 cytokines increased while the Th2 cytokines decreased. This can help us understand more better on the mechanisms of anti-HBV effect of oxymatrine.
Dong
Yu-hong
YH
Department of Infectious Disease, Peking University First Hospital, Beijing 100034, China.
Xi
Hong-li
HL
Tian
Feng
F
Kang
Ai-jun
AJ
Zhang
Nan-lin
NL
Yu
Min
M
Yu
Yan-yan
YY
Wang
Qin-huan
QH
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D016207:Cytokines / Q000097:blood*; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics*; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011807:Quinolizines; D000093842:Matrines
D000470:Alkaloids; D000998:Antiviral Agents; D016207:Cytokines; D006514:Hepatitis B Surface Antigens; D007371:Interferon-gamma; D016753:Interleukin-10; D007376:Interleukin-2; D015847:Interleukin-4; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines