Microbial hydroxylation of cinobufagin by Mucor spinosus.
Mucor spinosus has been employed for the biotransformation of cinobufagin (1) to afford three metabolites. On the basis of their physico-chemical data, the structures of the transformation products have been characterized as 1beta-hydroxy-cinobufagin (2), 12beta-hydroxy-cinobufagin (3) and 1beta,12beta-dihydroxy-cinobufagin (4), of which metabolites 2 and 4 are new compounds. In vitro cytotoxic activities of the biotransformation products and the substrate-cinobufagin have been assayed against four tumor cell lines of Bel 7420, BGC 823, HeLa and HL 60; they all showed cytotoxic activities.
Zhang
Wei
W
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Ye
Min
M
Qu
Gui-Qin
GQ
Wu
Wan-Ying
WY
Chen
Ying-Jie
YJ
Guo
De-An
DA
D001711:Biotransformation; D002018:Bufanolides / Q000378:metabolism*; D006900:Hydroxylation; D009089:Mucor / Q000378:metabolism*
D002018:Bufanolides; C002471:cinobufagin