title: Caprine herpesvirus-1 (CapHV-1) induces apoptosis in goat peripheral blood mononuclear cells.
abstract: Programmed cell death (PCD), or apoptosis, is initiated in response to various stimuli, including virus infection. A number of studies have shown that deregulation of apoptosis is an important feature of virus-induced immunosuppression for various viral diseases. In the present study, CapHV-1 was found to cause apoptosis in mitogen-stimulated as well as nonstimulated caprine peripheral blood mononuclear cells (PBMC). Apoptotic index, as quantified by fluorescent dyes, revealed a significant increase in the percentage of apoptotic cells at 24 and 48 h postinfection as compared to their respective noninfected controls. Apoptosis specific internucleosomal laddering in DNA from CapHV-1 infected PBMC was seen in agarose gel electrophoresis. No DNA fragmentation was observed in control noninfected PBMC. Virus-induced apoptosis was reduced by Z-VAD-FMK, an aspecific caspase inhibitor, by AC-DEVD-CHO (caspase-3-specific) and AC-VEID-CHO (caspase-6-specific) treatment. PCD in CapHV-1 infected peripheral blood mononuclear cells occurs at the G0/G1 phase of the cell cycle. However, penetration of virus particles and infection was not required for PCD, as UV-inactivated CapHV-1 induced apoptosis of mitogen-stimulated bovine peripheral blood mononuclear cells in vitro.
authors.affiliation: Department of Pathology and Animal Health, School of Veterinary Medicine, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. upagnini@unina.it
authors.forename: U
authors.initials: U
authors.lastname: Pagnini
authors.forename: S
authors.initials: S
authors.lastname: Montagnaro
authors.forename: E
authors.initials: E
authors.lastname: Sanfelice di Monteforte
authors.forename: F
authors.initials: F
authors.lastname: Pacelli
authors.forename: L
authors.initials: L
authors.lastname: De Martino
authors.forename: S
authors.initials: S
authors.lastname: Roperto
authors.forename: S
authors.initials: S
authors.lastname: Florio
authors.forename: G
authors.initials: G
authors.lastname: Iovane
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D061945:Caspase Inhibitors; D020169:Caspases / Q000276:immunology; D002453:Cell Cycle / Q000276:immunology; D053938:DNA Fragmentation / Q000276:immunology; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D015511:Goat Diseases / Q000097:blood / Q000276:immunology / Q000821:virology*; D006041:Goats; D006566:Herpesviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D007700:Kinetics; D007963:Leukocytes, Mononuclear; D009842:Oligopeptides / Q000494:pharmacology; D018140:Varicellovirus / Q000276:immunology*
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D009842:Oligopeptides; C099158:acetyl-aspartyl-glutamyl-valyl-aspartal; C110923:acetyl-valyl-isoleucyl-aspartyl-aldehyde; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D020169:Caspases