title: Diet is not responsible for the presence of several oxidatively damaged DNA lesions in mouse urine.
abstract: In order to eliminate the possibility that diet may influence urinary oxidative DNA lesion levels, in our experiments we used a recently developed technique involving HPLC pre-purification followed by gas chromatography with isotope dilution mass spectrometric detection. This methodology was applied for the determination of the lesions: 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 5-(hydroxymethyl)uracil (5HMUra) in the urine of mice fed with nucleic acid free diet and normal, unrestricted diet. The mean levels of 8-oxoGua, 8-oxodGuo and 5HMUra of the animals fed the normal diet reached the mean values of 15.6 +/- 3.5, 2.0 +/- 0.53 and 16.8 +/- 10.4 nmol/kg/24 h, After feeding the mice for 12 days with nucleic acid free diet the respective values were 18.8 +/- 4.6, 1.6 +/- 0.3 and 25.4 +/- 10.5 nmol/kg/24 h, respectively. The results clearly demonstrate that irrespective of the diet, the excretion rates were not statistically different during the course of feeding. The respective p values for the differences between lesions in the two types of diets were: 0.13 (8-oxoGua), 0.16 (8-oxodGuo), 0.18 (5-HMUra). Our results clearly indicate that diet does not contribute to urinary excretion of the lesions in mouse model.
authors.affiliation: Department of Clinical Biochemistry, The Ludwik Rydygier Medical University, Bydgoszcz, Karlowicza 24, 85-092, Bydgoszcz, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Rozalski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Siomek
authors.forename: Daniel
authors.initials: D
authors.lastname: Gackowski
authors.forename: Marek
authors.initials: M
authors.lastname: Foksinski
authors.forename: Christine
authors.initials: C
authors.lastname: Gran
authors.forename: Arne
authors.initials: A
authors.lastname: Klungland
authors.forename: Ryszard
authors.initials: R
authors.lastname: Olinski
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000821:Animal Feed; D000818:Animals; D004247:DNA / Q000652:urine*; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004032:Diet; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010432:Pentoxyl / Q000031:analogs & derivatives* / Q000652:urine
chemical_list: C008055:5-hydroxymethyluracil; C024829:8-hydroxyguanine; D006147:Guanine; D010432:Pentoxyl; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine