Limited effectiveness of antioxidants in the protection of yeast defective in antioxidant proteins.
Efficacy of several antioxidants in the protection of the yeast Saccharomyces cerevisiae mutants deficient in CuZnSOD and deficient in glutaredoxin 5 to growth restriction induced by oxidants was studied. Ascorbate and glutathione protected the Deltasod1 and Deltagrx5 mutants against the effects of t-butyl hydroperoxide and cumene hydroperoxide, Deltasod1 mutants against oxytetracycline and Deltagrx5 mutants against menadione and 2,2'-azobis-(2-amidinopropane). However, Tempol, Trolox and melatonin were much less effective, showing prooxidative effects and, at high concentrations, hampering the growth of the mutants in the absence of exogenous oxidants. These results point to a complication of cellular effects of antioxidants by their prooxidative effects and to the usefulness of cellular tests to evaluate the biological effectiveness of antioxidants.
Lewinska
Anna
A
Department of Biochemistry and Cell Biology, University of Rzeszów, ul. Cegielniana 12, PL 35-595 Rzeszów, Poland.
Bilinski
Tomasz
T
Bartosz
Grzegorz
G
D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D054477:Glutaredoxins; D008550:Melatonin / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D016877:Oxidants / Q000494:pharmacology; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1
D000975:Antioxidants; D054477:Glutaredoxins; D016877:Oxidants; D029701:Saccharomyces cerevisiae Proteins; D010088:Oxidoreductases; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D008550:Melatonin; D001205:Ascorbic Acid