title: 5-hydroxyuracil can form stable base pairs with all four bases in a DNA duplex.
abstract: NMR studies, UV-monitored melting experiments, and ab initio calculations show that 5-hydroxyuracil, produced by the oxidative de-amination of cytosines by reactive oxygen species, can form stable base-pairs with dA, dG, dC and dT residues in a DNA duplex, providing a basis for the in-vivo incorporation of 5-hydroxyuracil during DNA replication.
authors.affiliation: Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Varatharasa
authors.initials: V
authors.lastname: Thiviyanathan
authors.forename: Anoma
authors.initials: A
authors.lastname: Somasunderam
authors.forename: David E
authors.initials: DE
authors.lastname: Volk
authors.forename: David G
authors.initials: DG
authors.lastname: Gorenstein
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry*; D010084:Oxidation-Reduction; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D009705:Nucleosides; C045975:5-hydroxyuracil; D014498:Uracil; D004247:DNA