Synergistic interaction of adenosine diphosphate-epinephrine and epinephrine-collagen in aggregation of human platelets.
BACKGROUND
Efficient hemostasis in human body depends on a complicated series of events which actively involve blood platelets. Platelets exhibit diverse responses in a variety of agonists. In vivo, most of the agonists act in synergism, causing aggregation of platelets.


METHODS
In this study, the synergism between ADP-Epinephrine and Epinephrine-Collagen has been determined by means of turbidometric method, which measures changes in optical density of platelet suspension. The study was carried out at the department of Hematology, Armed Forces Institute of Pathology, Rawalpindi. The subthreshold concentrations of each of the agonists were established with the help of dose response curve. By adding these agonists in subthreshold concentrations, the synergism between ADP-Epinephrine and Epinephrine-Collagen was determined.


RESULTS
The combinations of these agonists in subthreshold levels showed the synergistic responses in causing platelet aggregation.


CONCLUSIONS
These findings indicate that the optimal platelet aggregation does occur between the above mentioned pairs of agonists even when each of the agonist is added to the other in subthreshold doses.
Razi
Muhammad Shamaun
MS
Department of Physiology, Shifa College of Medicine, Islamabad. drshamaun@yahoo.com
Butt
Idrees Farooq
IF
Ayub
Muhammad
M
Aslam
Muhammad
M
Hameed
Waqas
W
Badar
Ahmed
A
Afzal
Muhammad Nasir
MN
D000244:Adenosine Diphosphate / Q000502:physiology*; D000328:Adult; D018929:Cell Culture Techniques; D003094:Collagen / Q000502:physiology*; D004837:Epinephrine / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D010974:Platelet Aggregation / Q000502:physiology*; D012016:Reference Values
D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine