[Divergence of VP7 gene and VP4 gene variable regions of G1 rotaviruses isolated from different geographic locations around the world].
Group A rotaviruses are the most important etiologic agents of severe gastroenteritis in children, and as a result, the development of an effective vaccine against severe rotavirus diarrhea is a top public health priority. Previous studies indicated that G1 rotavirus are much more common in most countries around the world and development of G1--specific vaccine may be important in protection against human illness. In this report, we analyzed the VP7 gene and VP4 gene variable regions of 36 G1 rotavirus community strains collected from different geographic locations worldwide. Comparative analysis of the VP7 sequences showed that most of the amino acid substitutions of global strains occurred in and around previously identified epitope regions VR3, VR4, and VR6, suggesting that there are slight antigenic differences among these strains. Growtree phylogram using the VP7 amino acid sequences as an index grouped all strains into three clusters. The VP7 sequence of each cluster possesses distinctive amino acid residues which are peculiar to most members of the same group at a most positions. These data will be essential to achieve a much better understanding of molecular epidemiologic characteristics of G1 rotavirus around the world.
Jin
Q
Q
National Lab of Molecular Virology and Genetic Engineering, Beijing 100052.
Zheng
L Y
LY
Jing
H
H
Glass
R I
RI
Gentsch
J R
JR
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins