[Identification of G6PD gene mutations in several nationalities from Yunnan province].
OBJECTIVE
To analyze the G6PD gene mutations in several nationalities from Yunnan province.


METHODS
G6PD gene mutation of 29 samples from G6PD deficient patients were analyzed by using common and mis-matched primering PCR followed by restriction enzyme digestion. The samples were collected from Yunnan province among several nationalities.


RESULTS
In these 29 cases, 20 were of two known mutations, including 19 of 1388 (G-->A) and 1 of 1376 (G-->T) mutation. The 7 cases of national minorities, including 3 of Yi ([symbol: see text]), 2 of Bai ([symbol: see text]), 1 of Dai ([symbol: see text]), and 1 of Naxi ([symbol: see text]), were all 1388 mutations. No report of the G6PD gene 1388 mutation in these ethnic groups was published excepting for Dai.


CONCLUSIONS
The mutation probably had been prevalent before the emergence of these national minorities. The approximate rate of G6PD gene mutations provided in Yunnan province may, in some respects, help to interpret the molecular evolution.
He
Y
Y
Department of Medical Genetics, Sun Yat-sen University of Medical Sciences, Guangzhou 510089.
Du
C
C
Jiang
W
W
Wang
G
G
Ren
X
X
D044466:Asian People / Q000235:genetics*; D002681:China; D004252:DNA Mutational Analysis; D005838:Genotype; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000235:genetics; D006801:Humans; D008297:Male; D009154:Mutation; D020641:Polymorphism, Single Nucleotide
D005954:Glucosephosphate Dehydrogenase