[Amplification of 5' cDNA end of a disease resistance gene homolog using RACE system from soybean].
Based on part of a known partial cDNA sequence of a disease resistance gene homolog, KR3-1, obtained by screening a cDNA library from soybean, 5'-RACE-PCR was carried out with gene specific primers and universal primers. After the nested PCR reaction,an amplified fragment of 447 bp in length which overlapped the known KR3-1 sequence by 129 bp was obtained subsequently. Thus, a 5' cDNA end of KR3 was successfully cloned.
Wang
Bang-Jun
BJ
Central Laboratory, Southwest Agricultural University, Chongqing 400716, China bjwang2002@yahoo.com.cn
Wang
Qiang
Q
Zhang
Zhi-Gang
ZG
Zhang
Jin-Song
JS
Li
Xue-gang
XG