title: Global analysis of steady-state energy transfer measurements in membranes: resolution of structural and binding parameters.
abstract: A method has been developed allowing structural and binding parameters to be recovered by global analysis of two-dimensional array of steady-state RET data in the special case where energy acceptors distribute between aqueous and lipid phases while donors are embedded in the membrane at a known depth. To test the validity of this approach, correlation and error analyses have been performed using simulated data. To exemplify the method application to the membrane studies, energy transfer from anthrylvinyl-labeled phosphatidylcholine incorporated into mixed phosphatidylcholine/cardiolipin unilamellar vesicles to heme group of cytochrome c is analyzed.
authors.affiliation: Department of Biological and Medical Physics, V. N. Karazin Kharkiv National University, Kharkiv, Ukraine. domanov@pochta.ru
authors.forename: Yegor A
authors.initials: YA
authors.lastname: Domanov
authors.forename: Galina P
authors.initials: GP
authors.lastname: Gorbenko
authors.forename: Julian G
authors.initials: JG
authors.lastname: Molotkovsky
mesh_terms: D004735:Energy Transfer; D008567:Membranes, Artificial; D015203:Reproducibility of Results
chemical_list: D008567:Membranes, Artificial