[Preliminary study on oncogene MDM2 in acute leukemias].
OBJECTIVE
To explore the causes of MDM2 gene overexpression.


METHODS
The amplification and overexpression of MDM2 gene in 41 acute leukemia patients and two leukemic cell lines (K051 and HL60) were studied by using Southern blot, dot hybridyzation and RT-PCR techniques.


RESULTS
Overexpression of MDM2 gene was found in 51.2% (21/41) of the patients and HL60 cell line, but no amplification of the gene was found in all the patients and the two cell lines.


CONCLUSIONS
The level of MDM2 gene expression was related to the prognoses of the patients but not to FAB subtypes of acute leukemias.
Chao
H
H
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
Wang
L
L
Wang
J
J
Hao
Y
Y
Meng
Q
Q
Li
K
K
Feng
B
B
D000199:Actins / Q000235:genetics; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics* / Q000628:therapy; D008297:Male; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome
D000199:Actins; D012333:RNA, Messenger; D051736:Proto-Oncogene Proteins c-mdm2