Expression of PCNA and Ki-67 in the rat submandibular gland after one year cadmium intoxication--a preliminary study.
The aim of our study was to determine to what degree one-year exposure of female rats to cadmium at a dose of 5, 50 and 100 mg Cd/l affects cell proliferation in the submandibular gland, shown by PCNA and Ki-67 expression. In the present study, we found a positive nuclear reaction for PCNA in single cells in microscopic preparations of control rats. In Group I, an increase was observed in the number of PCNA-positive cells, compared to that in the control. In Group II, the number of PCNA-positive cells was markedly higher than that in Group I and in the control. In the submandibular glands of rats in Group III, the number of PCNA-positive cells was similar to that, found in Group II. However, Ki-67 expression was sporadically observed in control submandibular glands, but not in Groups I, II and III.
Czykier
E
E
Department of Histology and Embryology, Medical University of Białystok, Poland. czykier@amb.edu.pl
Dziecioł
J
J
D000818:Animals; D002105:Cadmium Poisoning / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D019394:Ki-67 Antigen / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen