title: Analgesic and topical anti-inflammatory activity of Hypericum canariense L. and Hypericum glandulosum Ait.
abstract: The present study investigates the analgesic and topical anti-inflammatory activities of the infusion, methanol extract and fractions of the aerial part in blossom of Hypericum canariense L. and Hypericum glandulosum Ait. in mice. The acetic acid-induced writhing test, tail flick test and the tetradecanoylphorbol acetate (TPA)-induced ear inflammation model in mice were used to determine these effects. Our findings show that oral administration of methanol extracts, and the aqueous, butanol and chloroform fractions of both species and the infusions of Hypericum glandulosum significantly inhibit acetic acid-induced writhing in mice. Only the infusion, methanol extract and butanol and chloroform fractions of Hypericum glandulosum were significantly active in the tail flick assay, suggesting that they may have central analgesic properties. On the other hand, the topical treatment of all extracts tested from both species, with the exception of the infusions and the Hypericum canariense aqueous fraction, significantly reduced the TPA-induced ear oedema. In conclusion, the results indicate analgesic and topical anti-inflammatory activities in mice for the Hypericum species studied.
authors.affiliation: Departamento de Medicina Física y Farmacología, Facultad de Farmacia, Universidad de la Laguna, c/ Astrofísico Francisco Sánchez s/n, 38071, La Laguna, Tenerife, Spain.
authors.forename: R M
authors.initials: RM
authors.lastname: Rabanal
authors.forename: C X
authors.initials: CX
authors.lastname: Bonkanka
authors.forename: M
authors.initials: M
authors.lastname: Hernández-Pérez
authors.forename: C C
authors.initials: CC
authors.lastname: Sánchez-Mateo
mesh_terms: D019342:Acetic Acid; D000284:Administration, Oral; D000287:Administration, Topical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D004423:Ear; D005260:Female; D035264:Flowers; D020902:Hypericum; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013623:Tail; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D013755:Tetradecanoylphorbol Acetate; D019342:Acetic Acid