Chromosomal radiosensitivity in breast cancer patients: influence of age of onset of the disease.
The age dependency of onset of the disease on chromosomal radiosensitivity of an unselected group of breast cancer patients (n=100) was investigated and compared to a group of healthy women (n=100). The chromosomal radiosensitivity was assessed with the G2 and the G0 micro-nucleus (MN) assay. For the G2 assay lymphocytes were irradiated in vitro with a dose of 0.4 Gy 60Co gamma-rays after 70 h incubation and chromatid breaks were scored in 50 metaphases. For the G0 MN assay lymphocytes were exposed in vitro to 3.5 Gy 60Co gamma-rays at low dose rate (LDR). 72 h post-irradiation cultures were arrested and micronuclei were scored in 1000 binucleate cells. The results demonstrated that the group of breast cancer patients was more radiosensitive than a population of healthy women and this with both the G2 and the G0 MN assay. Analyses of the G2 and MN response in different age groups of the breast cancer patients revealed no significant differences in mean G2 and MN scores and suggest that the age of onset of the disease has no effect on chromosomal radiosensitivity in unselected breast cancer patients. Correlations with different clinical parameters were also investigated.
Baeyens
Ans
A
Department of Anatomy, Embryology, Histology and Medical Physics, University of Ghent, L. Pasteurlaan 2, 9000 Ghent, Belgium.
Van Den Broecke
Rudy
R
Makar
Amin
A
Thierens
Hubert
H
De Ridder
Leo
L
Vral
Anne
A
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D017668:Age of Onset; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D019457:Chromosome Breakage; D002877:Chromosomes, Human / Q000528:radiation effects*; D005260:Female; D016195:G2 Phase; D006801:Humans; D015162:Micronucleus Tests; D008875:Middle Aged; D011836:Radiation Tolerance