title: Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories.
abstract: A set of Thatcher near-isogenic lines and two breeding lines were used to examine sequence tagged site (STS) markers linked to leaf rust resistance genes Lr9, Lr10, Lr19, Lr24, Lr28, Lr29, Lr35, and a simple sequenced repeat (SSR) marker for Lr39. The selected STS markers for resistance genes Lr9, Lr10, Lr19, Lr24 and Lr28 were identified in seven accessions by seven European laboratories. Near-isogenic lines of the spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9, Lr10, Lr19, Lr24 were identified in all seven laboratories as amplification products of 1100 bp, 310 bp, 130 bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, Lr10, Lr19, Lr24, Lr29, Lr35 and the SSR marker for Lr39 were robust and highly specific for these genes and will be useful in marker-assisted selection in wheat. However, the amplification product of 378 bp that corresponded with resistance gene Lr28 was detected in all accessions including genotypes lacking this gene in all seven laboratories. This marker needs to be improved.
authors.affiliation: Institute of Plant Genetics Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Błaszczyk
authors.forename: Jerzy
authors.initials: J
authors.lastname: Chełkowski
authors.forename: Victor
authors.initials: V
authors.lastname: Korzun
authors.forename: Jan
authors.initials: J
authors.lastname: Kraic
authors.forename: Frank
authors.initials: F
authors.lastname: Ordon
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Ovesná
authors.forename: Laszlo
authors.initials: L
authors.lastname: Purnhauser
authors.forename: Melinda
authors.initials: M
authors.lastname: Tar
authors.forename: Gyula
authors.initials: G
authors.lastname: Vida
mesh_terms: D001487:Basidiomycota; D017343:Genes, Plant; D005819:Genetic Markers; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics* / Q000382:microbiology; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D005819:Genetic Markers