PCR amplification with primers based on IS2404 and GC-rich repeated sequence reveals polymorphism in Mycobacterium ulcerans.
We describe a simple genotyping method for Mycobacterium ulcerans based on PCR amplification of genomic regions between IS2404 and a frequently repeated GC-rich sequence. Application of this method to a global collection produced 10 M. ulcerans genotypes corresponding to their geographic origin.
Ablordey
Anthony
A
Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp, Belgium.
Kotlowski
Roman
R
Swings
Jean
J
Portaels
Françoise
F
D000349:Africa / Q000453:epidemiology; D001208:Asia / Q000453:epidemiology; D001315:Australia / Q000453:epidemiology; D015373:Bacterial Typing Techniques; D017931:DNA Primers; D004251:DNA Transposable Elements; D020862:GC Rich Sequence; D005838:Genotype; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology* / Q000382:microbiology; D019911:Mycobacterium ulcerans / Q000145:classification* / Q000235:genetics*; D010219:Papua New Guinea / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D004251:DNA Transposable Elements