[The killing effect of IL-2 and IFN-alpha activated bone marrow on K562 leukemic cell].
OBJECTIVE
To evaluate the killing effect of IL-2 and IFN-alpha activated bone marrow cells on K562 cells.


METHODS
Semi-solid colony assay was used.


RESULTS
Bone marrow from leukemia patients in remission was activated in vitro with IL-2 for 3 days. The activated bone marrow (ABM) displayed killing effects of 0.31 approximately 2.30 logs on K562 cells, this killing effects was further increased to 0. 30 approximately > 3.15 logs when IFN-alpha added with IL-2 to the marrow for activation. IL-2 alone or in combination with IFN-alpha showed no inhibition of CFU-GM and K562 cells. Compared with IL-2 or IFN-alpha alone, the combination of the two cytokines could more effectively maintain the killing effect of ABM on leukemic cells.


CONCLUSIONS
IFN-alpha can augment the purging effect of IL-2 ABM and combination of the two cytokines can effectively maintain the cytotoxicity of ABM.
Li
C
C
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
Yan
W
W
Hao
Y
Y
Feng
S
S
Qiu
L
L
Liu
H
H
Han
J
J
Li
X
X
Feng
S
S
D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D020014:K562 Cells; D007938:Leukemia / Q000473:pathology*
D016898:Interferon-alpha; D007376:Interleukin-2