title: [Recombination and fusion expression of porcine defensin gene PBD-I in E. coli].
abstract: The porcine defensin gene PBD-I was amplified by RT-PCR, then the gene was inserted into expression vector PinPoint(TM) Xa-3. Recombinant plasmid named as ppd-1 was transformed into E.Coli JM109, which could effectively produce fusion protein induced with IPTG. The positive clone of PBD-I gene expressed 17kDa fusion protein by SDS-PAGE electrophoresis. Expression of PBD-I gene didn't increase distinctly along with time. The expression of PBD-I gene lays a foundation in research on antimicrobial activities and its mechanism of the defensin.
authors.affiliation: Laboratory Animal Center, Third Military Medical University, Chongqing 400038, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Luo
authors.forename: Hong
authors.initials: H
authors.lastname: Wei