[A probability analysis for HLA matching in adult stem cell transplantation treating nervous genetic diseases].
The aim of this study was to investigate the clinical feasibility of adult stem cell transplantation for lethal mono-gene inherited disease, Duchenne muscular dystrophy (DMD). A total of 30 blood samples from DMD patients were genotyped with HLA-A,-B and -DR alleles by means of polymerase chain reaction-reverse sequence specific oligonucleotide (PCR-RSSO). The HLA gene types in 30 DMD patients were compared with those of 668 unrelated donors from Umbilical Cord Blood Center of Guangdong Province and 34 910 unrelated donors from Chinese Bone Marrow Bank. The results showed that HLA gene of the DMD group was inherited in normal distribution. There was no striking difference of HLA-A, -B and -DR alleles expression between the DMD patients group and control healthy group. 25% of the DMD patients got suitable donors for stem cell transplantation, in which 15 patients found donors with >or= 5/6 HLA match at the Umbilical Cord Blood Center of Guangdong Province, i.e. occupying 50% of the total. Eight patients got 6/6 HLA matching donors at the Chinese Bone Marrow Bank, i.e. occupying 26% of the total. It is concluded that stem cells transplantation therapy for DMD patients is feasible, which will benefit these patients suffered from the lethal neuromuscular disease, and create a new way to treat this tough nervous system disease.
Xiao
Lu-Lu
LL
Guangzhou Blood Center, Guangzhou, 510095 China. lulux-640@hotmail.com
Chen
Wei
W
Zhang
Cheng
C
Liu
Zhuo-Lin
ZL
Ye
Xin
X
Zhang
Wei-Dong
WD
Yi
Yan
Y
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001771:Blood Banks; D002648:Child; D002675:Child, Preschool; D036101:Cord Blood Stem Cell Transplantation; D005240:Feasibility Studies; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D020388:Muscular Dystrophy, Duchenne / Q000097:blood / Q000235:genetics / Q000601:surgery*
D006680:HLA Antigens