Kinetics of MTT-formazan exocytosis in phagocytic and non-phagocytic cells.
MTT is taken up by cells by endocytosis and reduced to formazan in the endosomal/lysosomal compartment. Formazan is deposited intracellularly as blue granules and is later exocytosed as needle-like formazan crystals. The present study involves an analysis of the pattern of exocytosis of MTT in different cell types showing clearcut differences in the response that can be associated to their ability to phagocytose. To further assess the characteristics of the exocytic mechanism of MTT/formazan, different experimental conditions were assayed. When culture medium with decreasing serum concentration was used as a metabolic modulator no variations were observed in the proportion of cells with formazan crystals. Conversely, the markedly sensitivity of phagocytic cells to increasing concentrations of genistein constituted a remarkable difference with non-phagocytic cells. These results must be considered when the modulation of MTT exocytosis is used as a signal of the progress of human diseases.
Molinari
Beatriz L
BL
National Research Council, Buenos Aires, Argentina. beamolin@cnea.gov.ar <beamolin@cnea.gov.ar>
Tasat
Deborah R
DR
Palmieri
Mónica A
MA
Cabrini
Rómulo L
RL
D000818:Animals; D001943:Breast Neoplasms; D002460:Cell Line; D045744:Cell Line, Tumor; D003470:Culture Media; D011992:Endosomes / Q000502:physiology / Q000648:ultrastructure; D005089:Exocytosis / Q000502:physiology*; D005260:Female; D005562:Formazans / Q000493:pharmacokinetics*; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D010586:Phagocytes / Q000166:cytology* / Q000502:physiology; D013778:Tetrazolium Salts / Q000493:pharmacokinetics*
D003470:Culture Media; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; D019833:Genistein