title: [Protective effects of Cleistocalyx operculatus on lipid peroxidation and trauma of neuronal cells].
abstract: OBJECTIVE
  To observe the protective effects of Cleistocalyx operculatus on lipid peroxidation in rat liver microsomes and on the trauma of PC12 cells induced by H2O2.
  
  
  METHODS
  The mouse liver homogenate lipid peroxidation assay and PC12 Cell culture and Cell viability (MTT assay) were applied.
  
  
  RESULTS
  Cleistocalyx operculatus showed strong protective effects on lipid peroxidation in rat liver microsomes in a dose-dependent manner and exhibited potent protective effects on the trauma of PC12 cells induced by H2O2 (200 micromol x L(-1)) when the concentration reached 1.00 g x L(-1).
  
  
  CONCLUSIONS
  Cleistocalyx operculatus may be used as antioxidant to prevent or delay the pathogenesis of neural cell diseases.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Lu
authors.forename: Chang-bin
authors.initials: CB
authors.lastname: Du
authors.forename: Zi-bin
authors.initials: ZB
authors.lastname: Wu
authors.forename: Chun-lin
authors.initials: CL
authors.lastname: Ye
authors.forename: Jian-wen
authors.initials: JW
authors.lastname: Liu
authors.forename: Dong-zhi
authors.initials: DZ
authors.lastname: Wei
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D019610:Cytoprotection / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D027822:Myrtaceae / Q000737:chemistry; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D006861:Hydrogen Peroxide