[Clinical significance of detecting RNA and anti HEV antibody in convalesent sera in patients with acute HEV hepatitis].
OBJECTIVE
To investigate the anti hepatitis E virus (HEV) and HEV RNA in acute and convalescent sera of patients with NonA-E acute hepatitis.


METHODS
The serum samples were taken from 95 patients who were diagnosed as acute NonA-E hepatitis. Enzyme immunoassay (EIA) was used for detecting anti-HEV Immunoglobulin G (IgG, Genolable and Wantai EIA anti-HEV kits). RT-PCR amplification of HEV RNA was based on the open reading frame 2 region of HEV and the PCR products were sequenced.


RESULTS
Sera from 95 patients who were negative for anti-HEV in acute phase were followed up for 11-35 days to detect the anti-HEV antibody in recovery phase, 16/95 (16.84%) were positive for anti-HEV (wantai EIA anti-HEV kits). Ten (62.50%) were positive for HEV RNA in acute phase. Sequence analysis showed that 4 were HEV genotype. 6 were HEV genotype; 12/95 (12.50%) were positive for anti-HEV (Genolable EIA anti-HEV kits). Seven were positive for HEV RNA; 4 belonged to HEV genotype, 3 were HEV genotype.


CONCLUSIONS
It is significant and necessary to detect anti HEV antibody and HEV RNA in patients with HEV infection during acute phase and convalesent phase.
Li
Zhuo
Z
Beijing You An hospital Affiliated to Capital University of Medical Sciences, Beijing 100054, China.
Hao
Wa
W
Lan
Hai-yun
HY
Gu
Wen-jie
WJ
Wang
You-chun
YC
D000208:Acute Disease; D000595:Amino Acid Sequence; D001483:Base Sequence; D003289:Convalescence; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood*; D016751:Hepatitis E / Q000235:genetics / Q000276:immunology / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; C400971:ORF2 protein, Hepatitis E virus; D012367:RNA, Viral; D014764:Viral Proteins