title: Effects of halothane, enflurane, and isoflurane on plasma and erythrocyte antioxidant enzymes and trace elements.
abstract: Alterations of the normal redox balance in mammals might be attributed to increases of plasma free-radical concentrations and/or a disruption of the protective mechanisms. These conditions lead to damage to cellular structure by the mechanism of lipoperoxidation, particularly in the liver, kidney, and central nervous system. In this study, the effect of general anesthesia on the oxidative metabolism of human plasma and erythrocytes was investigated. Forty-five patients undergoing anesthesia by using halothane, enflurane, or isoflurane were included in this study. Blood samples were taken preoperatively, the first hour, the first day, and the third day after the operation. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme activities and trace elements such as cofactor copper (Cu), zinc (Zn) and selenium (Se) levels were measured in plasma and red blood cells. Our results showed that halothane and enflurane administration increased the plasma GSH-Px activity and reduced zinc levels. In addition, they lowered SOD and GSH-Px activities and trace element levels on erythrocytes. Isoflurane had no effect on plasma antioxidant enzymes, but, similar to the others, isoflurane decreased the plasma zinc levels, erythrocyte SOD and GSH-Px activities and trace element levels.
authors.affiliation: Department of Emergency Medicine, Division of Anesthesiology, Gazi University Medical Faculty, Ankara, Turkey.
authors.forename: Hülya
authors.initials: H
authors.lastname: Türkan
authors.forename: Neslihan
authors.initials: N
authors.lastname: Bukan
authors.forename: Ahmet
authors.initials: A
authors.lastname: Sayal
authors.forename: Ahmet
authors.initials: A
authors.lastname: Aydin
authors.forename: M Hakan
authors.initials: MH
authors.lastname: Bukan
mesh_terms: D000328:Adult; D000768:Anesthesia, General / Q000009:adverse effects*; D000975:Antioxidants / Q000032:analysis*; D003300:Copper / Q000097:blood; D004737:Enflurane; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000097:blood*; D006221:Halothane; D006801:Humans; D007530:Isoflurane; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D012643:Selenium / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood*; D014131:Trace Elements / Q000097:blood*; D015032:Zinc / Q000097:blood
chemical_list: D000975:Antioxidants; D014131:Trace Elements; D003300:Copper; D004737:Enflurane; D007530:Isoflurane; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium; D015032:Zinc; D006221:Halothane