title: Albumin is not present in the murine interphotoreceptor matrix, or in that of transgenic mice lacking IRBP.
abstract: OBJECTIVE
  The interphotoreceptor matrix mediates interactions between the retinal pigment epithelium, photoreceptors, and Muller cells. Each of these cells contributes to specific proteoglycans, proteins, and growth factors in the interphotoreceptor matrix. Some components, such as interphotoreceptor retinoid binding protein (IRBP), are virtually unique to the interphotoreceptor matrix. It has been proposed that serum albumin, thought to be present in the interphotoreceptor matrix, could act as a surrogate retinoid binding protein within the subretinal space of transgenic mice lacking interphotoreceptor retinoid binding protein. To address this question, we sought to determine whether albumin is present in the interphotoreceptor matrix of IRBP+/+ mice or IRBP-/- mice.
  
  
  METHODS
  We examined the distribution of albumin in IRBP-/- mice and IRBP+/+ mice using immunofluorescence and immunoperoxidase histochemistry.
  
  
  RESULTS
  The distribution of albumin within the corneal stroma, sclera, and capillaries is consistent with previous work. Serum albumin could not be detected in the interphotoreceptor matrix. The distribution of albumin in IRBP-/- mice was similar to that of their wildtype counterparts.
  
  
  CONCLUSIONS
  Serum albumin is not a component of the interphotoreceptor matrix of IRBP+/+ mice or IRBP-/- mice.
authors.affiliation: Department of Ophthalmology, University of Virginia, Charlottesville, USA.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Liao
authors.forename: Federico
authors.initials: F
authors.lastname: Gonzalez-Fernandez
mesh_terms: D000818:Animals; D002196:Capillaries / Q000378:metabolism; D003319:Corneal Stroma / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005136:Eye Proteins / Q000502:physiology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology; D010857:Pigment Epithelium of Eye / Q000166:cytology; D012177:Retinol-Binding Proteins / Q000172:deficiency / Q000502:physiology*; D012590:Sclera / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D012709:Serum Albumin; D000099214:Retinol-Binding Proteins, Interstitial