[In vitro effects of hematopoietic growth factors on CD34+ cell from patients with myelodysplastic syndromes].
OBJECTIVE
To investigate the role of recombinant human hematopoietic growth factors (HGFs) in the treatment of myelodysplastic syndromes (MDS) patients.


METHODS
CD34+ cells from 16 MDS patients were purified up to 96% approximately 99% by means of a immunomagnetic beads sorting system. The effects of individual and combination of HGFs on the proliferation and differentiation of the CD34+ cells were studied in semi -solid cultures.


RESULTS
CD34+ cells were impaired and none of the individual HGF (GM-CSF, IL-3, SCF) could effectively support their proliferation and differentiation. rh-SCF combined with GM-CSF, IL-3 or Epo could partly increase the colony forming capacity of MDS hematopoietic progenitor, however, an increase of undifferentiated blast cells colonies occurred in some high-risk MDS patients at the same time.


CONCLUSIONS
Proper combination of HGFs was essential for improving hematopoiesis in MDS patients.
Yang
X
X
Department of Hematology, Beijing Hospital.
Gu
X
X
Pei
X
X
Zhu
L
L
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D015672:Erythroid Precursor Cells / Q000187:drug effects / Q000473:pathology; D055014:Granulocyte-Macrophage Progenitor Cells / Q000187:drug effects / Q000473:pathology; D006410:Hematopoiesis / Q000187:drug effects; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000473:pathology*
D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors