[The antithrombin action of stichopus japonicus acid mucopolysaccharide (Sjamp) is mediated by heparin cofactor II].
OBJECTIVE
To further evaluate the antithrombin mechanism of stichopus japonicus acid mucopolysaccharide (Sjamp).


METHODS
The antithrombin mechanism of Sjamp was checked by assaying its effects on the thrombin activity in normal human pooled plasma,purified human heparin cofactor II (HC II) system and antithrombin III (AT III) system.


RESULTS
Sjamp inhibited thrombin activity in the presence of HC II with a second order rate constant of 1.56 x 10(7) m(-1) x min(-1), which was 4.6 times as much as that of AT III.


CONCLUSIONS
Sjamp was similar to dermatan sulfate both in the efficiency and in the mechanism of antithrombin.
Zhang
G
G
Department of Hematology, The second affiliated Hospital, Hunan Medical University, Changsha, 410011.
D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000494:pharmacology*; D015844:Heparin Cofactor II / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D047528:Stichopus / Q000737:chemistry*; D013917:Thrombin / Q000037:antagonists & inhibitors*
D006025:Glycosaminoglycans; D015844:Heparin Cofactor II; D000990:Antithrombin III; D013917:Thrombin