Anti-bone resorption activity of deer antler aqua-acupunture, the pilose antler of Cervus korean TEMMINCK var. mantchuricus Swinhoe (Nokyong) in adjuvant-induced arthritic rats.
Effect of deer antler aqua-acupunture (DAA), prepared from the pilose antler of Cervus korean TEMMINCK var. mantchuricus Swinhoe, a traditional immunosuppressive acupuncture, was evaluated to assess the reductions in bone mass, strength, and turnover in adjuvant-induced arthritic rats. For measuring the above parameters, a 20-day dosing experiment was performed using 6-week-old female Lewis rats. Arthritis was induced by injecting the adjuvant into the hind paw of the Lewis rats. The age-dependent increases in the body weight, lumbar bone mineral content and density (BMC and BMD) and compressive strength were disturbed in the arthritic rats. At 10 days, the histomorphometric parameters of bone formation (BFR/BS and BFR/BV) and the serum osteocalcin levels were significantly reduced compared with the baseline controls of Lewis rats. However, the BMC values corrected for body weight did not differ significantly between the arthritic and normal rats, and the bone minerals were not reduced when they were compared with the baseline controls. At 20 days, the parameters of bone minerals and strength of the lumbar body in the arthritic rats, both with and without correction for body weight, were significantly reduced compared with the baseline controls. The trabecular mineralizing surface remained significantly reduced and the osteoclast numbers were increased. DAA at the doses of 10, 20, 50 and 100 microg/kg, administered by Shinsu (B23) acupuncture daily from the start of the experiment, significantly prevented the development of the chronic paw edema at 20 days. The reductions in the parameters such as bone minerals, strength, and trabecular bone formation, and the increase in osteoclast number were alleviated by this DAA. Age-dependent increases in the lumbar height, disturbed by the adjuvant injection, were also maintained. These results indicated that a 20-day-period is necessary to obtain sufficient reductions in the bone mass and strength of the lumbar body concerning the model of secondary osteoarthritis in adjuvant arthritic rats. DAA was able to prevent these reductions by modulating the bone turnover in this arthritis model.
Kim
Kyung-Ho
KH
Department of Acupuncture, Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University and National Research Laboratory for Glycobiology, Kyungju, Kyungbuk 780-714, Republic of Korea.
Kim
Kap-Sung
KS
Choi
Byeong-Joon
BJ
Chung
Kang-Hyun
KH
Chang
Young-Chae
YC
Lee
Seung-Duk
SD
Park
Kwan-Kyu
KK
Kim
Hyung-Min
HM
Kim
Cheorl-Ho
CH
D026881:Acupuncture; D000367:Age Factors; D000818:Animals; D000999:Antlers / Q000737:chemistry*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology; D001688:Biological Products / Q000302:isolation & purification / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001862:Bone Resorption / Q000517:prevention & control*; D003670:Deer; D004487:Edema / Q000517:prevention & control; D005260:Female; D006614:Hindlimb; D008159:Lumbar Vertebrae / Q000187:drug effects / Q000473:pathology; D009161:Mycobacterium / Q000276:immunology; D015675:Osteocalcin / Q000097:blood; D010010:Osteoclasts / Q000187:drug effects / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew
D001688:Biological Products; D015675:Osteocalcin