Repair of oxidative DNA lesions in blood lymphocytes isolated from Sprague-Dawley rats; the influence of dietary intake of lignin.
Living organisms possess a variety of self-protective mechanisms which decrease the free radical attack on DNA and so reduce the risk of cancer. Protection of DNA by endogenous antioxidant systems may be significantly increased by numerous exogenously administered antioxidants. Many of them represent important dietary factors. Biopolymer lignin with its phenolic structure can be included into this group of micronutrients. The aim of the present work was to investigate: 1. the effect of biopolymer lignin, given to Sprague-Dawley (SD) rats in diet, on the level of oxidative DNA lesions induced by oxidative stress in freshly isolated peripheral blood lymphocytes in vitro and 2. the influence of lignin on kinetics of rejoining of DNA strand breaks induced in lymphocytes under these conditions. As model oxidative agents were used H2O2 and visible light in the presence of the photosensitizer Methylene Blue. We found out that dietary intake of lignin caused a significant decrease of H2O2-induced DNA strand breaks and visible light-induced oxidative DNA lesions in freshly isolated rat lymphocytes, but it did not influence the kinetics of rejoining of DNA strand breaks.
Lábaj
J
J
Lab. of Mutagenesis and Carcinogenesis, Cancer Research Institute of the Slovak Academy of Sciences, 833 91 Bratislava, Slovak Republic. juraj21@yahoo.com
Wsólová
L
L
Lazarová
M
M
Kosíková
B
B
Slamenová
D
D
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair; D004032:Diet; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008027:Light; D008031:Lignin / Q000494:pharmacology*; D008214:Lymphocytes / Q000000981:diagnostic imaging; D008297:Male; D008751:Methylene Blue; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D014463:Ultrasonography
D000975:Antioxidants; D008031:Lignin; D006861:Hydrogen Peroxide; D008751:Methylene Blue