[Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence].
In this study, RT-PCR product of the NS1 gene of dengue-2 virus isolated in China was inserted into the DNA of T-vector plasmid. DNAs of the positive clones, which were identified by using the blue/white spot and PCR, were digested with enzymes, indicating that the inserted fragments were the same sizes as those of the amplified NS1 genes. The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not changed.
Qin
E
E
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100850.
Yu
M
M
Yang
P
P
Si
B
B
Xu
P
P
Yan
G
G
D003001:Cloning, Molecular / Q000379:methods; D003716:Dengue Virus / Q000235:genetics*; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*
C079340:NS1 protein, Dengue virus type 2; D017361:Viral Nonstructural Proteins