[Study on transformation of snowdrop lectin gene to chrysanthemum and aphid resistance of the transgenic plants].
Agrobacterium-mediated transformation in chrysanthemum was studied to prevent the insect pest of aphid (Mizus persicae). The gna gene was successfully transferred into chrysanthemum by leaf dish, and 93 transgenic clones were obtained. The highest transformation frequency 11.21% was achieved on the optimization facts, which were medium YEB with pH5.6, bacterial concentration OD600 = 0.4, precultivation for one day, cocultivation for four days, the cocultivation media supplemented with GA3 0.5 mg/L and leaf explants growed for 45 days. The results from PCR and FQ-PCR analysis confirmed that gna gene was integrated into the genome of chrysanthemum plants. The insect bioassay with aphid showed that the aphid resistance of different transgenic plants was difference, and the rate of aphid population inhibition of them were from 10% to 84% with an average rate of 39.4%. The leaf-extracts from different transgenic plants showed varying actinties in red-blood cell bioassay.
Wang
Guan-Lin
GL
College of Life Science, Liaoning Normal University, Dalian 116029, China. guanlinwang@163.com
Liu
Yan-Hong
YH
Guo
Shao-Hua
SH
Wang
Yu
Y
Ji
Yan
Y
Fang
Hong-Jun
HJ
D000818:Animals; D001042:Aphids; D031202:Chrysanthemum / Q000235:genetics*; D037241:Mannose-Binding Lectins / Q000235:genetics*; D037121:Plant Lectins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C063117:snowdrop lectin