[Cloning and permanent expression in eukaryotic cells of rhTpo].
OBJECTIVE
To obtain recombinant human thrombopoietin (rhTpo).


METHODS
rhTpo gene was cloned from fetal liver mRNA by means of RT-PCR and subcloned into permanent expressing plasmid RC/CMV in order to form a recombinant permanent expressing plasmid RC/CMV/Tpo.


CONCLUSIONS
After being transferred into CHO cells, the eukaryotic permanent expression of rhTpo, which can enhance murine megakaryocyte colony growth in vitro, was obtained.
Cao
H
H
Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing.
Ge
Z
Z
Zhang
Q
Q
Liu
X
X
Sun
L
L
Zhang
X
X
Ding
Q
Q
D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013926:Thrombopoietin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D013926:Thrombopoietin