[Research progress of methods of SSR primers development].
SSRs is one of molecular markers technology based on DNA length polymorphism and an efficient tool for population genetic studies and primary genetic linkage maps construction. Because of a special primer marker, It's necessary to know a species DNA sequence in order to design primers for PCR testing. That is to say, there is a problem of SSR primer development. For the progress of SSR marker technology, the methods of developing SSR primer could be divided into four kinds: traditional constructing and screening genome library procedure, the SSR richment procedure, avoiding screening genome library procedure and database search procedure. This paper reviewed these four methods' operation processes and their advantages and disadvantages. In addition, transferability of SSR primers in closely related species were introduced in recent years.
Li
Ming-Fang
MF
National Key Bio-technology Laboratory for Tropical Crops, Chinese Academy of Tropical Agriculture Science, Haikou 571 101, China. mingfangl@hotmail.com
Zheng
Xue-Qin
XQ
D000818:Animals; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D020224:Expressed Sequence Tags; D015698:Genomic Library; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites
D017931:DNA Primers; D004247:DNA