title: Human IgA inhibits adherence of Acanthamoeba polyphaga to epithelial cells and contact lenses.
abstract: Specific anti-Acanthamoeba IgA antibodies have been detected in the serum and tears of patients and healthy individuals. However, the role of human secretory IgA antibodies in inhibiting the adherence of Acanthamoeba had not been previously investigated. Therefore, the purpose of this study was to purify secretory IgA from human colostrum and analyze its effect on the adherence of Acanthamoeba trophozoites to contact lenses and Madin-Darby canine kidney (MDCK) cells. IgA antibodies to Acanthamoeba polyphaga in colostrum of healthy women as well as in saliva and serum of healthy subjects were analyzed by ELISA and Western blot analysis. In serum, saliva, and colostrum, we detected IgA antibodies that recognized several antigens of A. polyphaga. In addition, colostrum and IgA antibodies purified from it inhibited adherence of A. polyphaga trophozoites to contact lenses and MDCK cells. These results suggest that IgA antibodies may participate in the resistance to the amoebic infection, probably by inhibiting the adherence of the trophozoites to contact lenses and corneal epithelial cells.
authors.affiliation: Department of Biochemistry, School of Medicine, National Polytechnic Institute, Plan de San Luis y Díaz Mirón, Mexico.
authors.forename: Rafael
authors.initials: R
authors.lastname: Campos-Rodríguez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Oliver-Aguillón
authors.forename: Luz M
authors.initials: LM
authors.lastname: Vega-Pérez
authors.forename: Adriana
authors.initials: A
authors.lastname: Jarillo-Luna
authors.forename: Dolores
authors.initials: D
authors.lastname: Hernández-Martínez
authors.forename: Saúl
authors.initials: S
authors.lastname: Rojas-Hernández
authors.forename: Marco A
authors.initials: MA
authors.lastname: Rodríguez-Monroy
authors.forename: Víctor
authors.initials: V
authors.lastname: Rivera-Aguilar
authors.forename: Arturo
authors.initials: A
authors.lastname: González-Robles
mesh_terms: D000048:Acanthamoeba / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion / Q000276:immunology; D002460:Cell Line; D003126:Colostrum / Q000276:immunology; D003261:Contact Lenses / Q000469:parasitology*; D004285:Dogs; D004847:Epithelial Cells / Q000469:parasitology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D012463:Saliva / Q000276:immunology
chemical_list: D007070:Immunoglobulin A