title: [Experimental research on immunological rejection of brain tissue allograft].
abstract: OBJECTIVE
  To investigate the occurrence of rejection in brain tissue transplantation.
  
  
  METHODS
  Ventral mesencephalon(VM) single cell suspensions from newborn rats were allografted into the striata of Parkinson's disease model recipient rats. Surviving animals were sacrificed at 2, 4 and 6 wk after transplantation, and the brain tissues were stained with HE or immunocytochemically to inspect the expression of tyrosine hydroxylase (TH) and major histocompatibility complex class II antigens (MHC II).
  
  
  RESULTS
  High MHC II expression was observed within and around the allografts compared with control at 2 wk after grafting. MHC II expression then decreased at 4 wk and at 6 wk only few immunopositive cells remained. The number of TH positive neurons was low at 2 wk, but increased at 4 and 6 wk after transplantation.
  
  
  CONCLUSIONS
  The brain is not an "immunologically privileged site" and graft rejection exists in brain tissue transplantation. This rejection maybe induced by MHC II. Therefore it is necessary to use immunosuppressants in brain transplantation.
authors.affiliation: Neurobiology Institute, Medical College of Southeast University, Nanjing 210009, China. myhanyang@ton.com
authors.forename: Yang
authors.initials: Y
authors.lastname: Han
authors.forename: Shui-Lin
authors.initials: SL
authors.lastname: Zeng
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Zhi-Nian
authors.initials: ZN
authors.lastname: Lei
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D016380:Brain Tissue Transplantation / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008297:Male; D009068:Movement; D017072:Neostriatum / Q000276:immunology / Q000378:metabolism; D010300:Parkinson Disease / Q000276:immunology / Q000473:pathology / Q000601:surgery; D051381:Rats; D014184:Transplantation, Homologous; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000949:Histocompatibility Antigens Class II; D014446:Tyrosine 3-Monooxygenase