Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay.
A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.
Zsak
L
L
Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944-0848, USA. lzsak@piadc.ars.usda.gov
Borca
M V
MV
Risatti
G R
GR
Zsak
A
A
French
R A
RA
Lu
Z
Z
Kutish
G F
GF
Neilan
J G
JG
Callahan
J D
JD
Nelson
W M
WM
Rock
D L
DL
D000357:African Swine Fever / Q000175:diagnosis*; D000358:African Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D015342:DNA Probes; D014066:Palatine Tonsil / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine / Q000821:virology; D019914:Taq Polymerase
D015342:DNA Probes; D019914:Taq Polymerase