Silole nanocrystals as novel biolabels.
A novel class of biofunctional silole nanocrystals with the potential to create highly sensitive immunoassay was firstly demonstrated. Biolabels were constructed by encapsulating nanocrystalline hexaphenylsilole [Ph2Si(CPh)4HPS] within ultrathin polyelectrolyte layers via the layer-by-layer (LbL) technique that provided an "interface" for the attachment of antibodies. A high ratio of fluorescent dyes to biomolecules (F/P ratio; 2.4 x 10(3)) was achieved without self-quenching problem. The aggregation-induced emission (AIE) feature offered silole biolabels the sensitivity 40- to 140-fold higher than that of a start-of-the-art immunoassay using directly fluorescent-labeled antibodies.
Chan
Cangel Pui-Yee
CP
Department of Chemistry, Biosensors and Bioelectronics Laboratory, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Haeussler
Matthias
M
Zhong Tang
Ben
B
Dong
Yongqiang
Y
Sin
King-keung
KK
Mak
Wing-cheung
WC
Trau
Dieter
D
Seydack
Matthias
M
Renneberg
Reinhard
R
D003460:Crystallization; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D036103:Nanotechnology; D010865:Pilot Projects; D013053:Spectrophotometry
D005456:Fluorescent Dyes