Analysis of thyroglobulin iodination by tandem mass spectrometry using immonium ions of monoiodo- and diiodo-tyrosine.
Peptides containing a monoiodo- or diiodo-tyrosine residue (monoiodo-Y, diiodo-Y) were found to generate abundant immonium ions following collision-induced dissociation at m/z 261.97 and 387.87 Da, respectively. These residue-specific marker ions are between about 140 mDa (monoiodo-Y) and 300 mDa (diiodo-Y) mass deficient relative to any other peptide fragment ions of unmodified peptides, qualifying them as highly specific marker ions for tyrosine iodination when analyzed by high resolution tandem mass spectrometry (MS/MS). Two new iodination sites (Y-364 and Y-2165) were pinpointed in bovine thyroglobulin by MS/MS using these iodotyrosine-specific marker ions and combined tryptic/chymotryptic digestion.
Salek
Mogjiborahman
M
Central Spectroscopy Unit, German Cancer Research Center, Heidelberg, Germany.
Lehmann
Wolf D
WD
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism; D017357:Cyclotrons; D004105:Diiodotyrosine / Q000737:chemistry*; D005240:Feasibility Studies; D007455:Iodine / Q000737:chemistry*; D007477:Ions; D008970:Molecular Weight; D007470:Monoiodotyrosine / Q000737:chemistry*; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013954:Thyroglobulin / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism
D007477:Ions; D010455:Peptides; D004105:Diiodotyrosine; D013954:Thyroglobulin; D007455:Iodine; D002918:Chymotrypsin; D014357:Trypsin; D007470:Monoiodotyrosine