title: [Peripheral nervous system in limited systemic sclerosis].
abstract: OBJECTIVE
  PNS is involved in Systemic Sclerosis (SSc) since the earliest phases. Our aim is to perform an ultrastructural study on skin PNS fibers in SSc.
  
  
  METHODS
  Skin biopsies were taken from forearms of 8 patients affected by limited SSc (lSSc) and 3 controls and processed for transmission electron microscopy (TEM). The semithin sections (2 mm) were observed at light microscope and optical fields were chosen for ultrathin sections (1 mm) preparation and TEM examination.
  
  
  RESULTS
  In lSSc skin, in the semithin sections, damaged areas are close to apparently spared areas. At TEM, in early lSSc patients, signs of inflammation and damaged microvessels are visible in derma. PNS fibers are no damaged. In advanced lSSc, fibrosis prevails on inflammation, and slight ultrastructural alterations of PNS fibers are evident in papillar derma.
  
  
  CONCLUSIONS
  PNS lesions are different in severity in lSSc according to the disease duration, resulting more severe in advanced than in early phase.
authors.affiliation: Dipartimento di Medicina Interna, Divisione di Reumatologia, Università di Firenze, 50139 Firenze, Italy. angedelr@tin.it
authors.forename: A
authors.initials: A
authors.lastname: Del Rosso
authors.forename: L
authors.initials: L
authors.lastname: Ibba Manneschi
authors.forename: A
authors.initials: A
authors.lastname: Tani
authors.forename: D
authors.initials: D
authors.lastname: Nosi
authors.forename: A
authors.initials: A
authors.lastname: Franca Milia
authors.forename: S
authors.initials: S
authors.lastname: Generini
authors.forename: S
authors.initials: S
authors.lastname: Guiducci
authors.forename: D
authors.initials: D
authors.lastname: Melchiorre
authors.forename: A
authors.initials: A
authors.lastname: Pignone
authors.forename: M
authors.initials: M
authors.lastname: Matucci Cerinic
mesh_terms: D001706:Biopsy; D016009:Chi-Square Distribution; D003627:Data Interpretation, Statistical; D005260:Female; D005355:Fibrosis; D016591:Histocytological Preparation Techniques; D006801:Humans; D008297:Male; D008833:Microcirculation; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009412:Nerve Fibers / Q000648:ultrastructure*; D017933:Peripheral Nervous System / Q000503:physiopathology*; D045745:Scleroderma, Limited / Q000473:pathology / Q000503:physiopathology*; D012867:Skin / Q000294:innervation* / Q000473:pathology; D013997:Time Factors