Neuromedin U receptor-2 mRNA and HCN channels mRNA expression in NMU-sensitive neurons in rat hypothalamic paraventricular nucleus.
We have characterized the neuromedin U (NMU)-sensitive neurons in the rat paraventricular nucleus (PVN) using whole-cell patch-clamp recordings and single-cell reverse transcription-multiplex polymerase chain reaction (single-cell RT-mPCR). Following completion of whole-cell recording, the NMU-sensitive neurons were examined for oxytocin (OT), vasopressin (VP), and corticotrophin-releasing hormone (CRH) mRNA expression using single-cell RT-mPCR. Of the NMU-sensitive neurons (n=23), 82% expressed OT mRNA, 9% expressed VP mRNA, 9% did not express the detected specific phenotypes mRNA. Further, the NMU-sensitive neurons (23/23) predominantly expressed NMU-receptor 2 (NMUR-2) mRNA, co-expressed HCN1 channel mRNA, HCN2 channel mRNA, and HCN3 channel mRNA but not HCN4 channel mRNA. These results suggest that NMU modulates the function of the PVN putative parvocellular neurons and is involved in the regulation of OTergic and VPergic neurons by enhanced HCN ion channels activity via NMU-receptor 2.
Qiu
De-Lai
DL
Department of Physiology, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake-cho, Miyazaki-gun, Miyazaki 889-1692, Japan.
Chu
Chun-Ping
CP
Tsukino
Hiromasa
H
Shirasaka
Tetsuro
T
Nakao
Hiroyuki
H
Kato
Kazuo
K
Kunitake
Takato
T
Katoh
Takahiko
T
Kannan
Hiroshi
H
D000818:Animals; D054815:Cyclic Nucleotide-Gated Cation Channels; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects* / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054815:Cyclic Nucleotide-Gated Cation Channels; C578667:Hcn1 protein, rat; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D008565:Membrane Proteins; D009479:Neuropeptides; D015221:Potassium Channels; D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter; C082444:neuromedin U receptor; C056900:neuromedin U