A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer.
Prostate cancer is among the most common tumors in industrialized nations. However, little is known about the molecular events underlying its development. In the present study we used suppressive subtraction hybridization (SSH) in combination with laser-assisted microdissection in order to compare gene expression between prostate carcinoma and the normal prostate proper. Both are mixed tissues which consist of an epithelial and a stromal compartment. We first compared mRNA (cDNA) expression by SSH and then used real-time quantitative RT-PCR analysis of microdissected tissue probes in order to verify differential expression of subtracted cDNA clones. We also used differentially expressed cDNAs for the synthesis of radiolabelled riboprobes in order to attribute differential expression to specific cell types in tissue sections by in situ hybridization. Using this approach we found an up-regulation of ubiquitin carboxyl extension protein 1 (UBCEP-1) mRNA in prostate carcinoma cells compared to the normal glandular epithelium of the prostate proper. UBCEP-1 mediated ubiquitin chain elongation may promote prostate carcinoma development by increasing via the proteasome pathway the degradation of proteins which are involved in growth inhibition or apoptosis.
Ko
Yon
Y
Medizinische Poliklinik, University of Bonn, D-53111 Bonn, Germany. yonko@uni-bonn.de
Hahn
Tanja
T
Lu
Hong
H
Ma
Zhan Lu
ZL
Chen
Jian
J
Rothe
Marcus
M
Florin
Alexandra
A
Fronhoffs
Stefan
S
Albers
Peter
P
Sachinidis
Agapios
A
Vetter
Hans
H
Kaminski
Annette
A
Behrens
Peter
P
Wellmann
Axel
A
Wernert
Nicolas
N
D000368:Aged; D017209:Apoptosis; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D003720:Densitometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D007834:Lasers; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D018075:RNA, Complementary / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics*; D013050:Spectrometry, Fluorescence; D035181:TATA-Box Binding Protein / Q000378:metabolism; D013997:Time Factors; D014452:Ubiquitins / Q000096:biosynthesis* / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D018075:RNA, Complementary; D012333:RNA, Messenger; C408062:RPS27a protein, human; D012269:Ribosomal Proteins; D035181:TATA-Box Binding Protein; D014452:Ubiquitins