title: [Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain].
abstract: OBJECTIVE
  To study the antigenicity of SARS associated coronavirus (CoV) spike S1 (12-672Aa) domain.
  
  
  METHODS
  BALB/c mice were immunized with a plasmid bearing codon-optimized SARS-CoV (Tor2 strain) S1 domain and then boosted with purified S1 protein; the SARS-CoV specific IgG antibody was tested by ELISA and neutralization antibody was determined by in vitro microneutralization assay.
  
  
  RESULTS
  S1 domain of SARS-CoV spike, which has been demonstrated harboring the receptor binding domain, successfully elicited SARS-CoV specific IgG antibody in mouse after combined immunization with DNA and purified S1 protein; the antibody elicited solely by S1 could potently neutralize SARS-CoV (HKU-39849) in vitro, 50% of 1 000 TCID50 SARS-CoV challenged cells were protected from viral infection by a 1:1499.68 dilution of mice sera immunized with S1 protein, but negative control sera showed no protection.
  
  
  CONCLUSIONS
  S1 domain of SARS-CoV spike protein, which is responsible for receptor binding, can efficiently and sufficiently induce highly potent neutralizing antibody in mice. This result suggested that S1 domain could be an effective subunit vaccines against SARS-CoV.
authors.affiliation: Department of Etiology, Institute of Basic Medical Science, CAMS and PUMC, Beijing 100005, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Yan-han
authors.initials: YH
authors.lastname: Li
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Li
authors.forename: Xin-ming
authors.initials: XM
authors.lastname: Tu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Guo-yong
authors.initials: GY
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Shu-hui
authors.initials: SH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D004622:Embryo, Mammalian; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D007668:Kidney / Q000166:cytology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins