Reduction of the transcription level of the mitochondrial genome in human glioblastoma.
Screening of human fetal brain cDNA library by glioblastoma (GB) and normal human brain total cDNA probes revealed 80 differentially hybridized clones. Hybridization of the DNA from selected clones and the same cDNA probes confirmed this difference for 38 clones, of which eight clones contained Alu-repeat inserts with increased levels in GB. Thirty clones contained cDNAs corresponding to mitochondrial genes for ATP synthase subunit 6 (ATP6), cytochrome c oxidase subunit II (COXII), cytochrome c oxidase subunit III (COXIII), NADH dehydrogenase subunit 1 (ND1), NADH dehydrogenase subunit 4 (ND4), and mitochondrial 12S rRNA. The levels of all these mitochondrial transcripts were decreased in glioblastomas as compared to tumor-adjacent histologically normal brain. Earlier we found the same for cytochrome c oxidase subunit I (COXI) Serial Analysis of Gene Expression (SAGE) showed lower content of the tags for all mitochondrial genes in GB SAGE libraries and together with our experimental data could serve as evidence of general inactivation of the mitochondrial genome in glioblastoma--the most malignant and abundant form of human brain tumor.
Dmitrenko
Vladimir
V
Department of Molecular Biology, Institute of Molecular Biology and Genetics, 150 Zabolotnogo str., 03143 Kiev, Ukraine.
Shostak
Katherina
K
Boyko
Oxana
O
Khomenko
Olexiy
O
Rozumenko
Vladimir
V
Malisheva
Tatiana
T
Shamayev
Mikhail
M
Zozulya
Yuriy
Y
Kavsan
Vadym
V
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics*; D002417:Cattle; D015342:DNA Probes; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D006824:Hybridization, Genetic; D008928:Mitochondria / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D003576:Electron Transport Complex IV