[Quantitative assessment of allospecific interleukin-2-secreting helper T lymphocyte in cord blood with limiting dilution assay].
OBJECTIVE
In order to investigate whether cord blood transplantation could induce severe graft versus host disease (GVHD) similar to that in bone marrow transplantation.


METHODS
The frequency of allospecific IL-2-secreting helper T lymphocyte (AIHTL) in 17 cord blood and 14 adult peripherial blood samples were detected by limiting dilution assay.


RESULTS
No statistically significant difference between the cord blood and adult peripheral blood AIHTLs was revealed.


CONCLUSIONS
Alloreactivity of cord blood was the same as that of adult blood. HLA typing and powerful immunosuppressive therapy should be adopted to reduce the incidence of GVHD in cord blood transplantation.
Jian
Q
Q
Huashan Hospital, Shanghai Medical University, Shanghai 200040.
Xie
Y
Y
Xie
H
H
D000328:Adult; D000918:Antibody Specificity; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D006086:Graft vs Host Disease / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007201:Indicator Dilution Techniques; D007231:Infant, Newborn; D007376:Interleukin-2 / Q000378:metabolism*; D007519:Isoantigens / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism*
D006680:HLA Antigens; D007376:Interleukin-2; D007519:Isoantigens