Heparin recovers AT1 receptor and its intracellular signal transduction in cultured vascular smooth muscle cells.
Although vascular smooth muscle cells (VSMCs) are widely used in cardiovascular research, their phenotypic change under various culture conditions is problematic to evaluate the experimental results obtained. The levels of angiotensin (Ang) type 1/2 (AT1/AT2) receptors as well as contractile and structural proteins are degraded through culture passages. The present study demonstrated that heparin recovered Ang receptors and differentiation markers, such as desmin, SM-22 and smooth muscle alpha-actin in VSMCs at the ninth passage. Heparin also potenciated Ang II-induced activation for ERK1/2 and p38. These results suggest a potential value of heparin-treated VSMCs as the model for analysis of Ang-mediated signal transduction under physiological condition.
Hashimoto
Tatsuo
T
Division of Cellular Pathobiology, Department of Pathology, Yokohama City University Graduate School of Medicine and School of Medicine, Yokohama, Japan. tatsuo_h@med.yokohama-cu.ac.jp
Kihara
Minoru
M
Sato
Keiko
K
Imai
Nozomi
N
Tanaka
Yutaka
Y
Sakai
Masashi
M
Tamura
Kouichi
K
Hirawa
Nobuhito
N
Toya
Yoshiyuki
Y
Kitamura
Hitoshi
H
Umemura
Satoshi
S
D000199:Actins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003893:Desmin / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D044140:Receptor, Angiotensin, Type 1 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000199:Actins; D003893:Desmin; D008840:Microfilament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; C051615:transgelin; D006493:Heparin; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases