Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1.
Gametogenetin (Ggn) is a testicular germ cell-specific gene specifically expressed from late pachytene spermatocytes through round spermatids. The function of gametogenetin protein 1 (GGN1) remains unknown. Here, we used the yeast two-hybrid approach to look for more GGN1 interacting proteins. We found that gametogenetin binding protein 1 (GGNBP1), gametogenetin binding protein 2 (GGNBP2) and ornithine decarboxylase antizyme 3 (OAZ3) were potential GGN1 interaction partners. We determined the regions mediating the interactions and further showed the interactions between the proteins in mammalian cells by colocalization and coimmunoprecipitation experiments. Our work suggested that GGN1, GGNBP1, GGNBP2 and OAZ3 could be involved in a common process associated with spermatogenesis.
Zhang
Jin
J
Beijing Institute of Biotechnology, Beijing 100071, PR China.
Wang
Yan
Y
Zhou
Yu
Y
Cao
Zhiguo
Z
Huang
Peitang
P
Lu
Baisong
B
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D006801:Humans; D047468:Immunoprecipitation; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism; D013735:Testicular Hormones / Q000737:chemistry / Q000378:metabolism*; D013737:Testis / Q000166:cytology; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C497525:GGNBP1 protein, mouse; C497527:GGNBP2 protein, mouse; C484251:Ggn protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; C497526:Oaz3 protein, mouse; D013735:Testicular Hormones