title: [Determination of protopine and isocorydine in root of Dactylicapnos scandens by HPLC].
abstract: OBJECTIVE
  To establish a HPLC method for determination of protopine and isocorydine in root of Dactylicapnos scandens.
  
  
  METHODS
  The separation was performed in a PHENOMENEX-C18 column with a mobile phase of methanol-0.2% phosphoric acid (adjusted to pH 7.0 with triethylamine)(50:50), The detection wavelength was at 254 nm and the flow rate was 0.8 mL x min(-1).
  
  
  RESULTS
  The average recovery of Protopine and Isocorydine was 97.9%, 98.6% respectively, and RSD 1.3%, 1.4%.
  
  
  CONCLUSIONS
  This method is accurate, simple and reliable. It can be used for quality control of D. scandens.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
authors.forename: Tian-qing
authors.initials: TQ
authors.lastname: Yan
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Yang
authors.forename: Tie-min
authors.initials: TM
authors.lastname: Ai
mesh_terms: D001060:Aporphines / Q000032:analysis*; D053119:Benzophenanthridines; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D028101:Papaveraceae / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D001060:Aporphines; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; C030168:isocorydine; C009093:protopine