Oligomeric compounds formed from 2,5-xylidine (2,5-dimethylaniline) are potent enhancers of laccase production in Trametes versicolor ATCC 32745.
Numerous chemicals, including the xenobiotic 2,5-xylidine, are known to induce laccase production in fungi. The present study was conducted to determine whether the metabolites formed from 2,5-xylidine by fungi could enhance laccase activity. We used purified laccases to transform the chemical and then we separated the metabolites, identified their chemical structure and assayed their effect on enzyme activity in liquid cultures of Trametes. versicolor. We identified 13 oligomers formed from 2,5-xylidine. (4E)-4-(2,5-dimethylphenylimino)-2,5-dimethylcyclohexa-2,5-dienone at 1.25 x 10(-5) M was an efficient inducer, resulting in a nine-fold increase of laccase activity after 3 days of culture. Easily synthesized in one step (67% yield), this compound could be used in fungal bioreactors to obtain a great amount of laccases for biochemical or biotechnological purposes, with a low amount of inducer.
Kollmann
Albert
A
Unité de Phytopharmacie et Médiateurs Chimiques, INRA, Route de Saint-Cyr, 78026 Versailles Cedex, France.
Boyer
François-Didier
FD
Ducrot
Paul-Henri
PH
Kerhoas
Lucien
L
Jolivalt
Claude
C
Touton
Isabelle
I
Einhorn
Jacques
J
Mougin
Christian
C
D000814:Aniline Compounds / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D004790:Enzyme Induction; D042845:Laccase / Q000096:biosynthesis*; D013997:Time Factors
D000814:Aniline Compounds; C016835:2,5-xylidene; D042845:Laccase