title: Yeast poly(A)-binding protein, Pab1, and PAN, a poly(A) nuclease complex recruited by Pab1, connect mRNA biogenesis to export.
abstract: In eukaryotic cells, pre-mRNAs undergo extensive processing in the nucleus prior to export. Processing is subject to a quality-control mechanism that retains improperly processed transcripts at or near sites of transcription. A poly(A) tail added by the normal 3'-processing machinery is necessary but not sufficient for export. Retention depends on the exosome. In this study, we identify the poly(A)-binding protein, Pab1, and the poly(A) nuclease, PAN, as important factors that couple 3' processing to export. Pab1 contains a nonessential leucine-rich nuclear export signal and shuttles between the nucleus and the cytoplasm. It can exit the nucleus either as cargo of exportin 1 or bound to mRNA. Pab1 is essential but several bypass suppressors have been identified. Deletion of PAB1 from these bypass suppressor strains results in exosome-dependent retention at sites of transcription. Retention is also seen in cells lacking PAN, which Pab1 is thought to recruit and which may be responsible for the final step of mRNA biogenesis, trimming of the poly(A) tail to the length found on newly exported mRNAs. The studies presented here suggest that proper loading of Pab1 onto mRNAs and final trimming of the tail allows release from transcription sites and couples pre-mRNA processing to export.
authors.affiliation: Department of Biochemistry, the Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: Ewan F
authors.initials: EF
authors.lastname: Dunn
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Hammell
authors.forename: Christine A
authors.initials: CA
authors.lastname: Hodge
authors.forename: Charles N
authors.initials: CN
authors.lastname: Cole
mesh_terms: D021581:Active Transport, Cell Nucleus; D005095:Exoribonucleases / Q000378:metabolism / Q000502:physiology*; D039102:Poly(A)-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D021382:Protein Sorting Signals; D021381:Protein Transport; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D005095:Exoribonucleases; D039102:Poly(A)-Binding Proteins; D021382:Protein Sorting Signals; D012322:RNA Precursors; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C450100:pab1 protein, S cerevisiae