title: Structural analysis of the O-antigen polysaccharide from Escherichia coli O152.
abstract: The structure of the O-antigen polysaccharide (PS) from Escherichia coli O152 has been determined. Component analysis together with 1H, 13C and 31P NMR spectroscopy were used to elucidate the structure. Inter-residue correlations were determined by 1H,31P COSY, 1H,1H NOESY and 1H,13C heteronuclear multiple-bond correlation experiments. The PS is composed of pentasaccharide repeating units with the following structure: [structure: see text]. The structure is similar to that of the O-antigen polysaccharide from E. coli O173. The cross-reactivity between E. coli O152 and E. coli O3 may be explained by structural similarities in the branching region of their O-antigen polysaccharides.
authors.affiliation: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Olsson
authors.forename: Kristina
authors.initials: K
authors.lastname: Lycknert
authors.forename: Roland
authors.initials: R
authors.lastname: Stenutz
authors.forename: Andrej
authors.initials: A
authors.lastname: Weintraub
authors.forename: Göran
authors.initials: G
authors.lastname: Widmalm
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry*
chemical_list: D019081:O Antigens