title: Effect of sono-abrasion in the microleakage of a pit and fissure sealant.
abstract: OBJECTIVE
  To assess the microleakage of a pit and fissure sealant after preparing enamel with sono-abrasion, used alone or associated with acid etching. This technique was compared with classical enamel preparations i.e. acid etching and diamond bur associated with etching.
  
  
  METHODS
  Ninety 3rd molars were used. In the mesial halves, the fissures were prepared with sono-abrasion and acid etching for 15 seconds. Then, the samples were randomly assigned to three groups of 30. They were either treated with acid etching alone (group 1), widened mechanically with a bur and etched for 15 seconds (group 2) or prepared with sono-abrasion alone (group 3). Then, the resin-based sealant was applied according to the manufacturer's recommendations. The teeth were thermocycled and placed in a 1% solution of methylene blue. The teeth showing microleakage and the means of infiltration were assessed with an image analysis system.
  
  
  RESULTS
  The poorest results were obtained with sono-abrasion alone which showed a greater number of specimens with microleakage (73.3%) (p < 0.001). They also showed the highest mean of microleakage (0.85+/-0.79 mm vs 0.1+/-0.26 for acid; 0.35+/-0.85 for bur associated with etching p < 0.0001); and 0.19+/-0.45 for sono-abrasion associated with etching.
  
  
  CONCLUSIONS
  No significant difference was noted between the three types of enamel preparation using etching. Sono-abrasion can be used for preparing dental enamel prior to sealing the tooth but it does not eliminate the need for etching.
authors.affiliation: Department of Public Health, Faculty of Odontology, University of Nice-Sophia Antipolis, Nice, France. Laurence.Lupi.Pegurier@wanadoo.fr
authors.forename: L
authors.initials: L
authors.lastname: Lupi-Pegurier
authors.forename: M
authors.initials: M
authors.lastname: Muller-Bolla
authors.forename: M F
authors.initials: MF
authors.lastname: Bertrand
authors.forename: G
authors.initials: G
authors.lastname: Ferrua
authors.forename: M
authors.initials: M
authors.lastname: Bolla
mesh_terms: D000134:Acid Etching, Dental; D020906:Air Abrasion, Dental; D003731:Dental Caries / Q000517:prevention & control*; D003743:Dental Enamel / Q000648:ultrastructure*; D003763:Dental Leakage / Q000517:prevention & control*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008964:Molar, Third; D010895:Pit and Fissure Sealants / Q000627:therapeutic use*; D019246:Tooth Preparation / Q000295:instrumentation / Q000379:methods*
chemical_list: D010895:Pit and Fissure Sealants