[Study on expression of adherent proteins related to regulation of hematopoiesis in long-term cultured human bone marrow stromal cells].
OBJECTIVE
To study the molecular basis of bone marrow stromal cell supporting hematopoiesis.


METHODS
Immunoelectron microscopic localization of adherent protein related to regulation of hematopoiesis was performed by immunocolloidal gold labelling technique in the adherent layer of long-term cultured human bone marrow stromal cells (LTCHBMSC).


RESULTS
Adherent proteins were found to be expressed on LTCHBMSC. Double labelling revealed co-expression of these proteins and some specific markers for endothelial cells (vWF) and fibroblasts (Fb-sp). It was suggested that endothelial cells and fibroblasts in LTCHBMSC could synthesize and secrete adherent proteins, which bound to the surface of stromal cells and extracellular matrix.


CONCLUSIONS
Adherent proteins and all kinds of hematopoietic growth factors secreted by stromal cells constitute a complex network supporting hematopoiesis.
Liu
J
J
Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Qi
S
S
de Wynter
E
E
Teata
N G
NG
Dexter
T M
TM
Allen
T D
TD
D001854:Bone Marrow Cells / Q000166:cytology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006046:Gold / Q000378:metabolism; D006410:Hematopoiesis; D006801:Humans; D008854:Microscopy, Electron; D017154:Stromal Cells / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D015815:Cell Adhesion Molecules; D006046:Gold