title: Oligonucleotides containing modified bases. I. Inhibition of DNA and RNA polymerases by partially thiolated oligocytidylic acids.
abstract: A series of oligomers of cytidylic acid were prepared and partially (6-9% of the bases) thiolated in the 5 positions. The modified oligomers showed increasing inhibition with increasing chain length of both the DNA polymerase-alpha from regenerating rat liver and the DNA-dependent RNA polymerase of E. coli, but the minimum chain length for observable inhibitory activity was 5 nucleotide units for the DNA polymerase-alpha and 16 units for the RNA polymerase.
authors.affiliation: Department of Medicinal Chemistry, State University of New York at Buffalo, Buffalo, NY 14214, USA.
authors.forename: Y K
authors.initials: YK
authors.lastname: Ho
authors.forename: M P
authors.initials: MP
authors.lastname: Kung
authors.forename: T J
authors.initials: TJ
authors.lastname: Bardos
mesh_terms: D000818:Animals; D003568:Cytidine Monophosphate / Q000737:chemistry / Q000494:pharmacology*; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009841:Oligonucleotides / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I; D003568:Cytidine Monophosphate