[Diversity of microbial genes in paddy soil stressed by cadmium using DGGE].
Variations of diversity of microbial genes in submerged paddy soil stressed by heavy metal cadmium were studied using modern molecular biotechnology which includes directly extracting total DNA from paddy soil, amplifying 16S rDNA and their V3 variable region by PCR, the denaturing gradient gel electrophoresis (DGGE). Two methods for extraction and purification of microbial DNA were compared. Bacterial communities were quantified by analyzing the DGGE band patterns. The genetic clusters and correlative comparison of bacterial communities were analyzed based on the DGGE finger-print. The results showed that there are some significant differences between bacterial communities in paddy soils treated with different concentrations of cadmium. The information about effect of cadium on microbial population based on molecular biological techniques are conformed with that from traditional methods, but that obtained about variations of microbial genes in paddy soil is much more than results based on the latter methods. It could provide a new way and foundation to research microbial gene diversity in contaminated environment.
Duan
Xue-jun
XJ
College of Life Science, Zhejiang University, Hangzhou 310029, China.
Min
Hang
H
D044822:Biodiversity; D002104:Cadmium / Q000032:analysis* / Q000633:toxicity; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D012275:Oryza; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000633:toxicity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium