Quantitative evaluation of immuno- and histochemical reaction intensity by spatial visualization techniques.
The aim of this study was an application of spatial visualization techniques for quantitative measurements of immuno- and histochemical reactions. For a quantitative histochemical study, specimens, collected from patients with chronic gastritis, were stained with paS/AB, while for immunohistochemical evaluation, specimens were used, collected from patients with chronic parathyroiditis and were analyzed with Ki-67 proliferation marker and apoptosis bcl-2 protein. The new technique permitted to obtain quantitative objective results. Statistical cluster analysis of those results indicated small groups of cases for reevaluation and supported the final diagnosis.
Górna
A
A
Laboratory of Morphometry and Medical Image Processing, University of Medical Sciences, Poznań, Poland.
Kaczmarek
E
E
Nieruchalska
E
E
Jarmołowska-Jurczyszyn
D
D
Majewski
P
P
D044963:Biopsy, Fine-Needle / Q000379:methods*; D005756:Gastritis / Q000473:pathology*; D006042:Goiter / Q000473:pathology*; D006801:Humans; D006965:Hyperplasia; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods; D010279:Parathyroid Diseases / Q000473:pathology