title: HLA-A gene polymorphism defined by high-resolution sequence-based typing in 161 Northern Chinese Han people.
abstract: Human leukocyte antigen (HLA) system is the most polymorphic region known in the human genome. In the present study, we analyzed for the first time the HLA-A gene polymorphisms defined by the high-resolution typing methods-sequence-based typing (SBT) in 161 Northern Chinese Han people. A total of 74 different HLA-A gene types and 36 alleles were detected. The most frequent alleles were A*110101 (GF=0.2360), A*24020101 (GF=0.1646), and A*020101 (GF=0.1553); followed by A*3303 (GF=0.1180), A*3001 (GF=0.0590), and A*310102 (GF=0.0404). The frequencies of following alleles, A*0203, A*0205, A*0206, A*0207, A*030101, A*2423, A*2601, A*3201, and A*3301, are all higher than 0.0093. The homozygous alleles include A*020101, A*110101, A*24020101 and A*310102. Heterozygosity (H), polymorphism information content (PIC), discrimination power (DP) and probability of paternity exclusion (PPE) of HLA-A in the samples were calculated and their values were 0.8705, 0.8491, 0.6014, and 0.9475, respectively. These results by SBT analysis of HLA-A polymorphism in Northern Chinese Han population, especially the allele subtypes character, will be of great interest for clinical transplantation, disease-associated study and forensic identification. Implementation of high-resolution typing methods allows a significantly wider spectrum of HLA variation including rare alleles. This spectrum will further be extensively utilized in many fields.
authors.affiliation: The State Laboratory of the Ministry of Health for Forensic Sciences, Xi'an 710061, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Yan
authors.forename: Ruilin
authors.initials: R
authors.lastname: Wang
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Li
authors.forename: Yajun
authors.initials: Y
authors.lastname: Deng
authors.forename: Dongying
authors.initials: D
authors.lastname: Wu
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhang
authors.forename: Lidong
authors.initials: L
authors.lastname: Wang
authors.forename: Chunrong
authors.initials: C
authors.lastname: Zhang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Huanming
authors.initials: H
authors.lastname: Yang
authors.forename: Shengbin
authors.initials: S
authors.lastname: Li
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D004252:DNA Mutational Analysis / Q000379:methods*; D005787:Gene Frequency; D005838:Genotype; D015234:HLA-A Antigens / Q000097:blood / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012699:Serology
chemical_list: D015234:HLA-A Antigens