[Immune response to HIV-2 gp105 DNA vaccine in mice].
OBJECTIVE
To explore the immune response of mice vaccinated with HIV-2 gp105 nucleic acid vaccine.


METHODS
pVAX1-gp105 recombinant DNA vaccine was constructed by inserting HIV-2 gp105 cDNA into an eukaryotic expression vector pVAX1. BALB/c mice were immunized with pVAX1-gp105, pVAX1 and PBS, respectively via intramuscular injection. Anti-HIV-2 antibody was detected by ELISA. The percentages of CD4(+) and CD8(+) T cell subsets from immunized mice were analyzed by flow cytometry and the specific killing activity of splenic CTLs was measured by lactate dehydrogenase (LDH) release assay.


RESULTS
The titer of specific antibody, the numbers of CD4(+) and CD8(+) T cells and cytotoxic activity of specific CTLs in pVAX1-gp105 vaccination group were obviously higher than those in control group (P<0.01, P<0.05 and P<0.01, respectively).


CONCLUSIONS
pVAX1-gp105 nucleic acid vaccine can elicit specific cellular and humoral immunities in mice.
Li
Zi-Jian
ZJ
Key Laboratory of Genetic Engineering, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China. lzjgy1995@163.com
Jin
Ning-Yi
NY
Jiang
Wen-Zheng
WZ
Zhang
Li-Shu
LS
D016915:AIDS Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics; D015483:HIV Antibodies / Q000276:immunology; D015498:HIV-2 / Q000276:immunology*; D007114:Immunization; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
D016915:AIDS Vaccines; D015483:HIV Antibodies; D019444:Vaccines, DNA; D054299:env Gene Products, Human Immunodeficiency Virus; C069790:gp105 envelope protein, Human immunodeficiency virus 2; D007770:L-Lactate Dehydrogenase