Aspergillus oryzae produces compounds inhibiting cholesterol biosynthesis downstream of dihydrolanosterol.
The formation of cholesterol synthesis inhibiting molecules by five different strains of the koji mold Aspergillus oryzae was studied. After growing these strains on a complex liquid medium we found in crude organic phase extracts and specific fractions there from compounds inhibiting cholesterol synthesis in human hepatic T9A4 cells in vitro at enzyme sites downstream of dihydrolanosterol. This was evidenced by using different radioactively labeled precursors, namely acetate, mevalonate, 24,25-dihydro-[24,25-(3)H2]-lanosterol or [3-(3)H]-lathosterol.
Hajjaj
Hassan
H
Nestlé Research Center, Nestec Ltd., Vers-chez-les-Blanc, P.O. Box 44, CH-1000 Lausanne 26, Switzerland.
Duboc
Philippe
P
Fay
Laurent B
LB
Zbinden
Irène
I
Macé
Katherine
K
Niederberger
Peter
P
D001236:Aspergillus oryzae / Q000378:metabolism*; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000378:metabolism
D003470:Culture Media; D004791:Enzyme Inhibitors; D007810:Lanosterol; D002784:Cholesterol