title: Evaluation of nested PCR in diagnosis of typhoid fever.
abstract: In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.
authors.affiliation: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. pradyot_micro@yahoo.co.in
authors.forename: Pradyot
authors.initials: P
authors.lastname: Prakash
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Mishra
authors.forename: Alok Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Gulati
authors.forename: Gopal
authors.initials: G
authors.lastname: Nath
mesh_terms: D000372:Agglutination Tests; D001769:Blood / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction / Q000379:methods*; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014435:Typhoid Fever / Q000175:diagnosis*
chemical_list: D003470:Culture Media