Binding effect of polychlorinated compounds and environmental carcinogens on rice bran fiber.
To accelerate the fecal excretion of polycyclic biphenyl (PCB), polychlorinated dibenzofurans (PCDFs), polychlorinated-p-dioxines (PCDDs) and various mutagens and carcinogens, their binding effect on rice bran fiber (RBF) was investigated for nine heterocyclic amines, six nitroarenes, 4-nitroquinoline-N-oxide, benzo[a]pyrene, furylfuramide, two kinds of flavonoid compounds and formaldehyde and ascorbic acid. PCBs, PCDFs and PCDDs suspended in nonane were incubated with RBF (10 mg/ml) at 37 degrees C and after centrifugation, unbound chemicals in the supernatant were analyzed by high-performance liquid chromatography (HPLC) and gas chromatography (GC). The binding effects on RBF were enhanced more than other dietary fibers (DFs), which were tested including corn, wheat bran, spinach, Hijiki (a kind of seaweed), sweet potatoes and burdock fibers. It was found that the binding effects were related to lignin contents. Binding of 3-amino-1(or 1,4)-dimethyl-5H-pyrido[4,3-b]indole (Trp-p-1 and Trp-p-2), food-derived carcinogens and 1-nitropyrene (1-NP), suspended in methanol, to RBF occurred within 10 min of incubation at 37 degrees C at pH 5-7, and decreased below pH 4; binding of food-derived carcinogens was pH dependent. The binding effects to RBF and pulp lignin were obtained at ratio of over 90%, while corn fiber and cellulose were at ratios of 4-30%. Polycyclic aromatic compounds were related to the number of rings, showing high binding effects to chemical structures with triple rings. Binding of 1-NP and PCB to RBF was not influenced in any aerobic and anaerobic bacterial cultures. It was also found that RBF was capable of binding even conjugates containing mutagens such as glucuronides and sulfates, as well as metabolites in urine. It was suggested, therefore, that mutagens and carcinogens were available for the fecal excretion of residual chemicals and their metabolites, and also for the fecal excretion of PCBs, PCDFs and related compound residues in patients of Yusho disease, who suffered food poisoning due to rice oil contaminated with PCB in Japan.
Sera
Nobuyuki
N
Department of Health Science, Fukuoka Institute of Health and Environmental Research, Mukaisano, Dazaifu 818-0135, Japan.
Morita
Kunimasa
K
Nagasoe
Masami
M
Tokieda
Hisako
H
Kitaura
Taeko
T
Tokiwa
Hiroshi
H
D001420:Bacteria, Aerobic / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000378:metabolism; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology; D002243:Carbolines / Q000737:chemistry; D002274:Carcinogens, Environmental / Q000494:pharmacology*; D000072338:Dibenzofurans, Polychlorinated; D004043:Dietary Fiber; D004147:Dioxins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008031:Lignin / Q000737:chemistry; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry; D012275:Oryza / Q000737:chemistry* / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D012907:Smoking / Q000652:urine
D001572:Benzofurans; D002243:Carbolines; D002274:Carcinogens, Environmental; D000072338:Dibenzofurans, Polychlorinated; D004043:Dietary Fiber; D004147:Dioxins; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D008031:Lignin; D011078:Polychlorinated Biphenyls; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole; C032668:1-nitropyrene