title: Catabolic role of a three-component salicylate oxygenase from Sphingomonas yanoikuyae B1 in polycyclic aromatic hydrocarbon degradation.
abstract: Sphingomonas yanoikuyae B1 possesses several different multicomponent oxygenases involved in metabolizing aromatic compounds. Six different pairs of genes encoding large and small subunits of oxygenase iron-sulfur protein components have previously been identified in a gene cluster involved in the degradation of both monocyclic and polycyclic aromatic hydrocarbons. Insertional inactivation of one of the oxygenase large subunit genes, bphA1c, results in a mutant strain unable to grow on naphthalene, phenanthrene, or salicylate. The knockout mutant accumulates salicylate from naphthalene and 1-hydroxy-2-naphthoic acid from phenanthrene indicating the loss of salicylate oxygenase activity. Complementation experiments verify that the salicylate oxygenase in S. yanoikuyae B1 is a three-component enzyme consisting of an oxygenase encoded by bphA2cA1c, a ferredoxin encoded by the adjacent bphA3, and a ferredoxin reductase encoded by bphA4 located over 25kb away. Expression of bphA3-bphA2c-bphA1c genes in Escherichia coli demonstrated the ability of salicylate oxygenase to convert salicylate to catechol and 3-, 4-, and 5-methylsalicylate to methylcatechols.
authors.affiliation: Department of Biology, Institute of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Okyoung
authors.initials: O
authors.lastname: Cho
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Choi
authors.forename: Gerben J
authors.initials: GJ
authors.lastname: Zylstra
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Seong-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Joon H
authors.initials: JH
authors.lastname: Lee
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Sohn
authors.forename: Gi-Seok
authors.initials: GS
authors.lastname: Kwon
authors.forename: Young Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Eungbin
authors.initials: E
authors.lastname: Kim
mesh_terms: D001673:Biodegradation, Environmental; D002396:Catechols / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism; D006841:Hydrocarbons, Aromatic / Q000737:chemistry / Q000378:metabolism; D005810:Multigene Family; D009281:Naphthalenes / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism*; D012459:Salicylates / Q000378:metabolism; D020578:Sphingomonas / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D002396:Catechols; D005288:Ferredoxins; D006841:Hydrocarbons, Aromatic; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012459:Salicylates; D010105:Oxygenases; D005287:Ferredoxin-NADP Reductase