[Analysis of partial sequence of the gene for a novel Br protein].
A strain of halophilic archaeum AB1 was isolated and purified from Aibi Lake located in the north of Xinjiang Uygur Autonomous Region. Partial DNA fragment encoding a bacteriorhodopsin (Br) protein as well as 16S rRNA of AB1 was amplified by PCR, and their nucleotide sequences were determined subsequently. On the basis of homology and phylognetic analysis about 16S rRNA gene (16S rDNA), it could be speculated that the strain AB1 is a novel member of the genus Natronococcus. The hydropathy analysis of Br fragment revealed that the AB1 Br had a transmembrane heptahelical structure similar to that of other Brs. On the other hand, homology alignment using the deduced partial amino acid sequence of Br protein of AB1 with other Br proteins showed that AB1 Br protein is obviously different to others. These facts indicated that the Br in halophilic archaeum AB1 is a new Br protein.
Xu
Xue-Wei
XW
College of Life Sciences, Zhejiang University, 310027 Hangzhou, China. xxwju@hotmail.com
Wu
Min
M
Ruan
Hong
H
Huang
Wei-Da
WD
Tohty
Dilbar
D
D000595:Amino Acid Sequence; D001436:Bacteriorhodopsins / Q000235:genetics*; D001483:Base Sequence; D019641:DNA, Archaeal / Q000235:genetics*; D008969:Molecular Sequence Data; D019634:Natronococcus / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S; D001436:Bacteriorhodopsins