title: [Cloning and sequencing of p35 gene from Bombyx mori nuclear polyhedrosis virus strain Shandong].
abstract: According to the 5' and 3' untranslated region sequences of p35 gene from Bombyx mori nuclear polyhedrosis virus isolate T3, a pair of primers was designed employing computer analysis. With the primers,polymerase chain reaction (PCR) was performed and a 1080 bp fragment was obtained from BmNPV strain Shandong. Sequencing result showed that it was p35 gene (GenBank accession number: AY157746), and it included an open reading frame of 900 bp, encoding 299 amino acids with Mr=34.91 kDa and pI=6.40. BLAST analysis indicated that there were seven bases and three amino acids difference between p35 from BmNPV strain Shandong and that from BmNPV isolate T3.
authors.affiliation: Department of Sericultural Silk Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Jiang
authors.forename: Wan-Fang
authors.initials: WF
authors.lastname: Zhong
authors.forename: Ping-Zhong
authors.initials: PZ
authors.lastname: Cai
authors.forename: Xing-Yao
authors.initials: XY
authors.lastname: Xu
authors.forename: Wen-Zhao
authors.initials: WZ
authors.lastname: Yan