[Morphological and cytochemitry features of CD34+ hematopoietic cells isolated from human bone marrow].
OBJECTIVE
To explore the cytomorphological and cytochemitry features of CD34+ hematopoietic cells.


METHODS
Highly purified CD34+ hematopoietic cells isolated from human bone marrow by a two-step method of immunomagnetic beads-FACS sorting were comprehensively examined.


CONCLUSIONS
CD34+ hematopoietic cells could be sorted into three types in light of morphology and cytochemical staining. Type I was considered as candidates for stem cells,which were most blastlike in morphology and slightly larger than lymphocytes in size with negative reactions to all cytochemical stainings. Type II was considered as multipotent progenitor cells, with sizes similar to small lymphocytes and negative reactions to all cytochemical stainings. Type III was identified as committed progenitor cells whose sizes were heterogeneous and whose reactions to cytochemical stainings ranged from +/- to + +.
Xi
Y
Y
Department of Immunology, Affiliated 307 Hospital, Academy of Military Medical Sciences, Beijing 100039.
Li
X
X
Zhang
S
S
Tang
P
P
Mao
N
N
Wei
W
W
Kong
F
F
D018952:Antigens, CD34 / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation; D006410:Hematopoiesis; D006801:Humans; D013194:Staining and Labeling
D018952:Antigens, CD34