Induction of matrix metalloproteinase-9 in mice during Toxocara canis larvae migration.
The relationships between inflammation in organs with Toxocara canis larval migration and matrix metalloproteinase-9 (MMP-9) were investigated following the infection of mice with 1,000 infective eggs. Gelatinase activity was defined by gelatin zymography, optimum pH, inhibitor specificity and Western blot analysis. MMP-9 activity was present in the lungs, liver, muscles, and brain during T. canis larval migration. This enzyme had a molecular weight of about 94 kDa and showed maximum activity in the pH range of 6-8. The increased MMP-9 proteinases coincided with larval recovery and the degree of inflammation among the four organs. These results suggest that MMP-9 may be associated with the inflammatory reaction to larval toxocariasis during early migration, and may therefore be a useful marker during T. canis larvae migration.
Lai
S C
SC
Department of Parasitology, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, 402, Taichung , Taiwan.
Chen
K M
KM
Chen
H C
HC
Lee
H H
HH
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000469:parasitology / Q000473:pathology; D004790:Enzyme Induction; D006863:Hydrogen-Ion Concentration; D007249:Inflammation; D007814:Larva / Q000502:physiology; D008099:Liver / Q000201:enzymology / Q000469:parasitology / Q000473:pathology; D008168:Lung / Q000201:enzymology / Q000469:parasitology / Q000473:pathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000201:enzymology / Q000469:parasitology; D009928:Organ Specificity; D017166:Toxocara canis / Q000502:physiology*; D014120:Toxocariasis / Q000201:enzymology* / Q000469:parasitology* / Q000473:pathology
D020780:Matrix Metalloproteinase 9