mRNA for chosen pro- and anti-inflammatory cytokines in T-lymphocytes in paediatric leukemias and lymphomas--a preliminary report.
We assessed mRNA for chosen pro- and anti-inflammatory cytokines in T-lymphocytes of peripheral blood in paediatric patients with leukemias and lymphomas. Levels of four different cytokine mRNAs (IFN-gamma, IL-10, IL-4, TGF-beta) were determined by the real-time PCR technique. In the whole examined group, at the time of diagnosis, we noted lower amounts of mRNA for TGF-beta1, comparing to respective values in the control patients. In the ALL group, we observed the following: 1) at the time of diagnosis: lower amounts of mRNA for IL-4 and for TGF-beta1, comparing to respective values in the control group; 2) lower amounts of mRNA for IL-10 after remission induction, comparing to the time of diagnosis. In our opinion, "immunedysregulation" in lymphoproliferative diseases in children is not caused by IFN-gamma deficiency. The deficit of anti-inflammatory cytokines, i.e., IL-4, TGF-beta, with higher amounts of IL-10, suggests their role in cancer development.
Luczyński
W
W
Department of Paediatric Oncology, Medical University of Białystok, Poland. vlodek@amb.edu.pl
Kovalchuk
O
O
Krawczuk-Rybak
M
M
Malinowska
I
I
Mitura-Lesiuk
M
M
Matysiak
M
M
Kowalczyk
J
J
Chyczewski
L
L
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000235:genetics*; D006689:Hodgkin Disease / Q000235:genetics / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics*; D012074:Remission Induction; D013601:T-Lymphocytes / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000235:genetics
D016207:Cytokines; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10