Water-soluble rylene dyes as high-performance colorants for the staining of cells.
The synthesis of perylene and terrylene chromophores carrying a single poly(ethylene oxide) chain is presented. These chromophores reveal a strong solvatochromic behavior: High fluorescence in nonpolar solvents and weak fluorescence in polar solvents which is mainly attributed to aggregation. Therefore, such chromophores are attractive candidates as sensitive fluorescent probes reflecting the polarity of their environment. In particular, their suitability for the staining of cellular membranes is presented in detail.
Weil
Tanja
T
Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany. Tanja.Weil@merz.de
Abdalla
Moustafa A
MA
Jatzke
Claudia
C
Hengstler
Jan
J
Müllen
Klaus
K
D000042:Absorption; D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D002725:Chloroform / Q000737:chemistry; D006224:Cricetinae; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry; D015394:Molecular Structure; D010569:Perylene / Q000737:chemistry; D011083:Polycyclic Compounds / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012680:Sensitivity and Specificity; D012995:Solubility; D013194:Staining and Labeling; D014050:Toluene / Q000737:chemistry; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D015335:Molecular Probes; D011083:Polycyclic Compounds; D014867:Water; D014050:Toluene; D011092:Polyethylene Glycols; D010569:Perylene; D002725:Chloroform