title: Antioxidative ability in a linoleic acid oxidation system and scavenging abilities against active oxygen species of enzymatic hydrolysates from pollen Cistus ladaniferus.
abstract: The enzymatic hydrolysates from pollen Cistus ladaniferus were digested and prepared using three kinds of enzymes (pepsin, trypsin, and papain) and the antioxidative properties were investigated. The yields, total phenolic contents, and protein contents of these hydrolysates were as follows: yields (about 21-45%), total phenolics (10.39-14.33 microg/mg sample powder), and proteins (129.62-137.35 microg/mg sample powder), respectively. The hydrolysates possessed strongly antioxidative and scavenging abilities against reactive oxygen species. The present studies revealed that hydrolysates from honeybee-collected pollen are of benefit not only to the materials of health food diets, but also to patients with various diseases such as cancer, cardiovascular diseases, and diabetes.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Agriculture, Hokkaido 099-2493, Japan. t1nagai@seibutu.bioindustry.nodai.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagai
authors.forename: Reiji
authors.initials: R
authors.lastname: Inoue
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Suzuki
authors.forename: Takao
authors.initials: T
authors.lastname: Myoda
authors.forename: Toshio
authors.initials: T
authors.lastname: Nagashima
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds; D029758:Cistus / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000737:chemistry; D007202:Indicators and Reagents / Q000494:pharmacology; D019787:Linoleic Acid / Q000378:metabolism*; D016877:Oxidants / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D010206:Papain / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry; D019800:Phenol / Q000737:chemistry; D010851:Picrates / Q000494:pharmacology; D011058:Pollen / Q000737:chemistry / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000737:chemistry; D013997:Time Factors; D014357:Trypsin / Q000737:chemistry
chemical_list: D000578:Amidines; D000975:Antioxidants; D001713:Biphenyl Compounds; D007202:Indicators and Reagents; D016877:Oxidants; D010851:Picrates; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; D019800:Phenol; C046728:2,2'-azobis(2-amidinopropane); D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D014357:Trypsin; D010206:Papain; D010434:Pepsin A; D010100:Oxygen