An improved method for checking HTS/uHTS liquid-handling systems.
An efficient method is presented to determine precision and accuracy of multichannel liquid-handling systems under conditions near to application. The method consists of gravimetrical determination of accuracy and optical determination of precision based on the dilution of absorbing and fluorescent dye solutions in microplates. Mean delivery volume per well can be determined with precision better than a 0.04% coefficient of variation (CV). Optical signal precision, CV(S), is improved by multiwavelength measurements. Precision of absorbance measurement yields a better resolution than precision of fluorescence measurement (0.3% and 1.5%, respectively), indicating that absorbance measurements should be preferred. From CV(S), an upper bound of the precision of the volumes delivered is derived. Method performance is demonstrated with the dispenser CyBi-Drop and the pipettor CyBi-Well using different ejection principles; with commonly used fluids; with 96-, 384-, and 1536-well microplates; and with photometric and fluorometric indicators. Precision of the volumes delivered, as obtained with optimized methods, all plate formats, and both devices, is better than 2% CV with 2 microL set volume and about 1% CV with higher set volumes.
Rhode
Heidrun
H
Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, D-07740 Jena, Germany. Heidrun.Rhode@mti.uni.uni-jena.de
Schulze
Margarete
M
Renard
Simon
S
Zimmermann
Peter
P
Moore
Thomas
T
Cumme
Gerhard A
GA
Horn
Anton
A
D002138:Calibration; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000032:analysis; D008722:Methods; D010783:Photometry; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry
D005456:Fluorescent Dyes; D012996:Solutions