title: Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings.
abstract: An inexpensive enzyme-linked immunosorbent assay method for human immunodeficiency virus type 1 quantitation, ultrasensitive p24 antigen assay (Up24), was compared with RNA viral load assay (VL). Up24 had 100% sensitivity of detection at a viral load of >/=30,000, with sensitivity of 46.4% at a viral load of <30,000 (232 specimens from 65 seropositive subjects). The assay was highly reproducible, with excellent correlation between duplicates and among three laboratories.
authors.affiliation: Global AIDS Program, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mail Stop A-12, Atlanta, GA 30333, USA. rrespess@cdc.gov
authors.forename: Richard A
authors.initials: RA
authors.lastname: Respess
authors.forename: Ada
authors.initials: A
authors.lastname: Cachafeiro
authors.forename: David
authors.initials: D
authors.lastname: Withum
authors.forename: Susan A
authors.initials: SA
authors.lastname: Fiscus
authors.forename: Daniel
authors.initials: D
authors.lastname: Newman
authors.forename: Bernard
authors.initials: B
authors.lastname: Branson
authors.forename: Oliviero E
authors.initials: OE
authors.lastname: Varnier
authors.forename: Kim
authors.initials: K
authors.lastname: Lewis
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Dondero
mesh_terms: D000328:Adult; D016655:HIV Core Protein p24 / Q000097:blood*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D016655:HIV Core Protein p24; D012367:RNA, Viral