title: [Rapid amplification of cDNA end and its application].
abstract: Rapid amplification of cDNA end (RACE) technique is a method of which the 3' and 5' fragments of cDNA can be rapidly obtained. In this review, the advantages and shortcomings RACE manipulation were pointed out and some important technical points in RACE protocols in the previous literatures were summarized.
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, the State Key Laboratory of Integrated Management of Pest Insects and Rodents, Beijing 100 080, China. wangsl@panda.ioz.ac.cn
authors.forename: Shao-Li
authors.initials: SL
authors.lastname: Wang
authors.forename: Cheng-Fa
authors.initials: CF
authors.lastname: Sheng
authors.forename: Chuan-Ling
authors.initials: CL
authors.lastname: Qiao
mesh_terms: D018076:DNA, Complementary / Q000235:genetics*; D005784:Gene Amplification; D015723:Gene Library; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger