title: Regulation of interleukin-8 secretion in human intestinal epithelial Caco-2 cells by alpha-humulene.
abstract: It is well known that various cytokines such as interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha are expressed and secreted from intestinal epithelial cells and that these cytokines affect the immune cells beneath the intestinal epithelial monolayers. As the secretion of these cytokines is likely to be regulated by food-derived substances, we focused on those food substances which regulate the secretion of IL-8 in human intestinal epithelial Caco-2 cells. 72 food samples extracted with 40% ethanol were tested, and the extracts of peppermint and dokudami significantly increased the IL-8 secretion. Among the compounds known to be contained in peppermint and dokudami, alpha-humulene substantially increased the IL-8 secretion.alpha-Humulene had no significant effect on the secretion of such other soluble factors as TNF-alpha, IL-1beta, IL-6, or NGF, suggesting that the effect of alpha-humulene was specific for IL-8 secretion. The expression level of IL-8 mRNA was significantly increased by treating with alpha-humulene. These results suggest that the secretion of IL-8 by alpha-humulene is regulated at the transcriptional level.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. asatsu@mail.ecc.u-tokyo.ac.jp
authors.forename: Hideo
authors.initials: H
authors.lastname: Satsu
authors.forename: Tomoko
authors.initials: T
authors.lastname: Matsuda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Toshimitsu
authors.forename: Akira
authors.initials: A
authors.lastname: Mori
authors.forename: Tatsumasa
authors.initials: T
authors.lastname: Mae
authors.forename: Misuzu
authors.initials: M
authors.lastname: Tsukagawa
authors.forename: Mikio
authors.initials: M
authors.lastname: Kitahara
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D045744:Cell Line, Tumor; D003110:Colonic Neoplasms; D005504:Food Analysis; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology*; D000081222:Monocyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: D016209:Interleukin-8; D000081222:Monocyclic Sesquiterpenes; D012717:Sesquiterpenes; C042686:humulene