Effects of sleep deprivation, nicotine, and selenium on wound healing in rats.
Effects of sleep deprivation (SD), nicotine, and selenium (Se) on wound healing were studied in 50 male rats (Sprague-Dawley strain). Full-skin-thickness burns were produced in animals. Then, SD, nicotine, and Se administrations were applied to animals in different groups. Wound healing was assessed by pathological analysis of wound by counting fibroblasts, capillary vessels, polymorphonuclear leucocytes (PNLs), and by measuring radiolabeled immunoglobulin G (IgG) amount in wound area by radio-pharmaceutical and immunoscintigraphic procedures. The number of fibroblasts and capillary vessels were higher in control and Se groups than in sleep deprivation and nicotine groups, and the number of PNLs and the radiolabeled polyvalent IgG levels were higher in SD and nicotine groups than in control and Se groups. The results suggest that SD and nicotine may delay wound healing and that Se supplementation may accelerate wound healing by preventing nicotine-induced oxidative stress and lipid peroxidation.
Gümüştekín
Kenan
K
Department of Physiology, Medical School, Atatürk University, Erzurum, Turkey.
Seven
Bedrí
B
Karabulut
Nezíhe
N
Aktaş
Omer
O
Gürsan
Nesrín
N
Aslan
Sahín
S
Keleş
Mustafa
M
Varoglu
Erhan
E
Dane
Senol
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004195:Disease Models, Animal; D004347:Drug Interactions; D005347:Fibroblasts / Q000187:drug effects; D007074:Immunoglobulin G / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D016719:Radioimmunodetection / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000293:injuries; D012892:Sleep Deprivation; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000975:Antioxidants; D007074:Immunoglobulin G; D018722:Nicotinic Agonists; D009538:Nicotine; D012643:Selenium