title: Protection of mitochondrial system by Hippophae rhamnoides L. against radiation-induced oxidative damage in mice.
abstract: The whole extract of the fresh berries of Hippophae rhamnoides L. (RH-3), which has been reported to provide protection to whole mice, various tissues, cells and cell organelles against lethal irradiation, was further investigated for its effects on mitochondria isolated from mouse liver. Superoxide anion, reduced (GSH) and oxidized glutathione (GSSG) levels, NADH-ubiquinone oxidoreductase (complex I), NADH-cytochrome c oxidoreductase (complex I/II), succinate-cytochrome c oxidoreductase (complex II/III), mitochondrial membrane potential (MMP), lipid peroxidation (LPx) and protein oxidation (PO) were determined for RH-3-mediated radioprotective manifestation. Pre-irradiation treatment of mice with RH-3 (30 mg kg(-1,) i. p.; single dose; -30 min) significantly inhibited the radiation-induced increase in superoxide anions, GSSG, thiobarbituric acid reactive substances (TBARS), complex I, complex I/III activity and MMP maximally at 4 h (P < 0.05). This treatment inhibited the oxidation of proteins (P < 0.05) at all the time periods studied here. This study suggests that pre-irradiation treatment of mice with RH-3 protects the functional integrity of mitochondria from radiation-induced oxidative stress.
authors.affiliation: Radiation Biology Division, Institute of Nuclear Medicine and Allied Sciences, Brig. S. K. Mazumdar Road, Delhi-110 054, India. goelharish@hotmail.com
authors.forename: Harish Chandra
authors.initials: HC
authors.lastname: Goel
authors.forename: Damodar
authors.initials: D
authors.lastname: Gupta
authors.forename: Shobha
authors.initials: S
authors.lastname: Gupta
authors.forename: A P
authors.initials: AP
authors.lastname: Garg
authors.forename: Madhu
authors.initials: M
authors.lastname: Bala
mesh_terms: D000818:Animals; D042967:Electron Transport Complex I / Q000378:metabolism; D005638:Fruit / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D031281:Hippophae / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D010936:Plant Extracts; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D011837:Radiation-Protective Agents; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014916:Whole-Body Irradiation
chemical_list: D010936:Plant Extracts; D011506:Proteins; D011837:Radiation-Protective Agents; D013481:Superoxides; D013384:Succinate Cytochrome c Oxidoreductase; D042967:Electron Transport Complex I; D005978:Glutathione