[Endothelial nitric oxide synthase gene transfected endothelial cells inhibit smooth muscle cell proliferation and platelet aggregation early in vitro].
OBJECTIVE
To test the function of endothelial cells transfected eNOS gene.


METHODS
To test the function of eNOS-overexpressing endothelial cells inhibiting smooth muscle cell proliferation and platelet aggregation in vitro by colorimetry, MTT and (3)H-TdR permeate, respectively, in four different endothelial cells groups.


RESULTS
The platelet aggregation is significantly decreasing between the groups of EC and eNOS at the different time (P < 0.05), is 42.2 and 32.6 separately at the time of 120 h. The SMC proliferation is significantly decreasing between the groups of EC and eNOS at the different time (P < 0.05). It is 0.28 and 0.22 by MTT test, 4691 and 3995 by (3)H-TdR permeate separately at the time of 120 h.


CONCLUSIONS
eNOS-overexpressing endothelial cells inhibited significantly smooth muscle cells proliferation and platelet aggregation in vitro; which shows powerful effect 48 hours post transfection and lasts up to 120 hours at the least; and were held back by L-NAME.
Xue
Guan-Hua
GH
Department of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Zhang
Ji-Wei
JW
Zhang
Lan
L
Zhang
Hao
H
Zhang
Bai-Gen
BG
D000818:Animals; D002450:Cell Communication; D049109:Cell Proliferation; D018920:Coculture Techniques; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D009569:Nitric Oxide / Q000378:metabolism* / Q000502:physiology; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D010974:Platelet Aggregation / Q000502:physiology*; D014162:Transfection
D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III