[The effect of opsonins on erythrocytes phagocytosis in the vitreous--laboratory research (Part A)].
OBJECTIVE
We performed experimental investigations in vitro into the opsonization of Rh-positive erythrocytes by IgG anti-RhD antibodies and phagocytosis of erythrocytes in the vitreous body.


METHODS
The vitreous has been taken from the corpses of young people. Anti-RhD antibodies came from preparations 'Gamma anti-D 150'. 1% Erytrocyte suspension of ccDee phenotype has been applied. Phagocytosis assay was performed in the medium of the vitreous and in Hank's solution.


RESULTS
Phagocytosis assay performed in the vitreous and in Hank's solution did not display statistically significant differences.


CONCLUSIONS
The investigations in vitro presented here, proved that immunological reactions are really occurring in the vitreous body. They provide a base for the explanation of activity mechanism of anti-RhD antibodies injected intraoculary in cases of hemorrhages into vitreous body.
Raczyńska
Krystyna
K
Katedry i Kliniki Chorób Oczu Akademii Medycznej w Gdańsku.
Iwaszkiewicz-Bilikiewicz
Barbara
B
D000328:Adult; D002448:Cell Adhesion / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D012107:Research Design; D012204:Rh-Hr Blood-Group System / Q000276:immunology; D014822:Vitreous Body / Q000276:immunology*
C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D009895:Opsonin Proteins; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen