title: Hepatitis C virus core protein, cytochrome P450 2E1, and alcohol produce combined mitochondrial injury and cytotoxicity in hepatoma cells.
abstract: OBJECTIVE
  Alcohol consumption exacerbates liver injury in chronic hepatitis C, and enhanced mitochondrial oxidative stress is one possible mechanism. The aim of this study was to determine whether hepatitis C virus core protein and alcohol-inducible cytochrome P450 2E1 contribute to reactive oxygen species production and cytotoxicity in human hepatoma cells.
  
  
  METHODS
  Huh-7 cells expressing core protein, cytochrome P450 2E1, or both were exposed to 0.1 mmol/L tertiary butyl hydroperoxide, tumor necrosis factor alpha, and/or 25 mmol/L ethanol. Cytotoxicity, reactive oxygen species production, glutathione content, and mitochondrial membrane potential were measured.
  
  
  RESULTS
  Expression of core/cytochrome P450 2E1 synergistically enhanced cell death induced by either tertiary butyl hydroperoxide or tumor necrosis factor alpha. After tertiary butyl hydroperoxide treatment, total reactive oxygen species production was increased more than 3-fold compared with cells that did not express core and cytochrome P450 2E1. Mitochondrial depolarization and reduced glutathione depletion occurred as well, and cell death was prevented by inhibition of mitochondrial permeability transition or caspase activity. Confocal microscopy showed that the mitochondria themselves were the origin of the reactive oxygen species. In the absence of core/cytochrome P450 2E1 expression, mitochondrial changes and cell death did not occur. Ethanol treatment further decreased mitochondrial reduced glutathione content and exacerbated mitochondrial reactive oxygen species production, depolarization, and cell death. All these effects were prevented by the antioxidant N -acetylcysteine.
  
  
  CONCLUSIONS
  Mitochondrial reactive oxygen species production is induced by hepatitis C virus core and cytochrome P450 2E1, resulting in a reduction of mitochondrial antioxidant capacity and sensitivity to oxidants and tumor necrosis factor alpha. Alcohol further depletes mitochondrial reduced glutathione, which exacerbates depolarization and cell death. Sensitization of mitochondria to oxidative insults is thus a potential mechanism for alcohol-related exacerbation of liver injury in chronic hepatitis C.
authors.affiliation: Department of Neuroscience and Cell Biology, University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555-0632, USA
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Otani
authors.forename: Masaaki
authors.initials: M
authors.lastname: Korenaga
authors.forename: Michael R
authors.initials: MR
authors.lastname: Beard
authors.forename: Kui
authors.initials: K
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Qian
authors.forename: Lori A
authors.initials: LA
authors.lastname: Showalter
authors.forename: Aman K
authors.initials: AK
authors.lastname: Singh
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Steven A
authors.initials: SA
authors.lastname: Weinman
mesh_terms: D000438:Alcohols / Q000009:adverse effects*; D006528:Carcinoma, Hepatocellular; D016923:Cell Death / Q000502:physiology; D045744:Cell Line, Tumor; D002908:Chronic Disease; D019392:Cytochrome P-450 CYP2E1 / Q000276:immunology*; D000431:Ethanol / Q000009:adverse effects*; D005978:Glutathione; D006526:Hepatitis C / Q000503:physiopathology*; D006801:Humans; D008113:Liver Neoplasms; D008928:Mitochondria / Q000187:drug effects* / Q000276:immunology; D008954:Models, Biological; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha; D014758:Viral Core Proteins / Q000276:immunology*
chemical_list: D000438:Alcohols; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1; D005978:Glutathione