Non-aqueous capillary electrophoresis for simultaneous separation and determination of three major active components in traditional medicinal preparations.
A simple and sensitive non-aqueous capillary electrophoresis method has been developed for simultaneous assay of three bioactive components (puerarin, daidzein and wogonin) in three traditional medicinal preparations for the first time. Optimum separation of the analytes was obtained on a 47 cm x 75 microm i.d. capillary using a non-aqueous buffer system of 20% acetonitrile, 25 mm ammonium acetate and apparent pH 9.00, with applied voltage and capillary temperature of 20 kV and 16 degrees C, respectively. The relative standard deviations (RSDs) of the migration times and the peak areas of the three analytes were in the ranges 2.5--4.0% and 3.2--3.9%, respectively. Detection limits of puerarin, daidzein and wogonin were 0.090, 0.145 and 0.090 microg mL(-1), respectively. In the tested concentration range, good linear relationships (correlation coef fi cients: 0.9998 for puerarin, 0.9998 for daidzein and 0.9978 for wogonin) between peak areas and concentrations of the analytes were observed. This method has been successfully applied to simultaneous determination of the three bioactive components with recoveries from 91.0 to 114.0%.
Li
Cunhong
C
Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
Zhang
Jiyou
J
Chen
Anjia
A
Xie
Jianping
J
Chen
Xingguo
X
Hu
Zhide
Z
D000085:Acetates; D000097:Acetonitriles; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D044950:Flavanones / Q000032:analysis* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification*; D000432:Methanol; D015203:Reproducibility of Results; D013696:Temperature
D000085:Acetates; D000097:Acetonitriles; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D007529:Isoflavones; C004742:daidzein; C085514:wogonin; C018824:ammonium acetate; D000432:Methanol; C032159:acetonitrile; C033607:puerarin