title: [GM-CSF regulates the Vp16 induced leukemic cell apoptosis].
abstract: OBJECTIVE
  To explore the regulatory effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on the apoptosis of leukemic cells induced by Vp16.
  
  
  METHODS
  High expression retrovirus vector, N2A/CMV/GM-CSF, was constructed and transferred into human leukemic cell line HL-60, and Vp16 (final concentration 10microg/ml) was administered to transferred or nontransferred HL-60 cells.
  
  
  RESULTS
  After Vp16 treatment, characteristic changes for apoptosis emerged in HL-60 cells. However, these findings did not emerge in GM-CSF gene transferred HL-60 cells.
  
  
  CONCLUSIONS
  GM-CSF can suppress the leukemic cell apoptosis induced by Vp16.
authors.affiliation: Institute of Hematology, Union Hospital, Tongji Medical University, Wuhan 430022.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Zou
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Hou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D005047:Etoposide / Q000494:pharmacology*; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*
chemical_list: D005047:Etoposide; D016178:Granulocyte-Macrophage Colony-Stimulating Factor