title: [Preparation and characterization of nanoemulsion].
abstract: OBJECTIVE
  To prepare nanoemulsion-encapsulated BSA-FITC (NEBSA-FITC), study its characteristics, and measure its uptake by dendritic cells (DCs) and peritoneal macrophages.
  
  
  METHODS
  NEBSA-FITC was prepared by a method of interfacial polymerization.The encapsulation rate, drug-carrying capacity and stability of the nanoemulsion were determined by Sephadex-G100 chromatography. The shape and size of NEBSA-FITC were observed under electron microscope. The uptake of NEBSA-FITC by DCs and macrophage cells was detected by FACS and laser confocal microscopy.
  
  
  RESULTS
  The mean size of NEBSA-FITC was (25+/-10) nm. The encapsulation rate was 91%, the drug-carrying capacity was 0.091 g/L and NEBSA-FITC had a good stability. The FACS analysis showed that DCs and macrophage cells could take in more NEBSA-FITC than free BSA. The observation under laser confocal microscope found that NEBSA-FITC was located in the cytoplasm of DCs.
  
  
  CONCLUSIONS
  Nanoemulsion can be efficiently taken by DCs and macrophage cells, and therefore may be promising efficient carrier of APCs-targeted antitumor vaccine.
authors.affiliation: Department of Pathology, Fourth Military Medical University, Xi'an 710032, China. sunyuj@fmmu.edu.cn
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Sun
authors.forename: Dao-Cheng
authors.initials: DC
authors.lastname: Wu
authors.forename: Yun-Xin
authors.initials: YX
authors.lastname: Cao
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Sui
mesh_terms: D000818:Animals; D001692:Biological Transport; D002417:Cattle; D003713:Dendritic Cells / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism*; D004655:Emulsions; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D036103:Nanotechnology; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate