title: Cloning and characterization of a molt-inhibiting hormone-like peptide from the prawn Marsupenaeus japonicus.
abstract: Recently, it was demonstrated by PCR amplification that an additional molt-inhibiting hormone (MIH)-like peptide was present in the kuruma prawn Marsupenaeus japonicus. In this study, a cDNA encoding this peptide designated Pej-MIH-B was cloned. The Pej-MIH-B gene was expressed strongly in the nerve cord, and weakly in the eyestalk. It was possible to isolate Pej-MIH-B from the sinus glands in the eyestalks. The recombinant Pej-MIH-B expressed in Escherichia coli showed low molt-inhibiting activity, but did not exhibit hyperglycemic activity. These results suggest that Pej-MIH-B does not function as MIH or CHH intrinsically, but may have some unknown functions.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8657, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohira
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Katayama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tominaga
authors.forename: Tetsu
authors.initials: T
authors.lastname: Takasuka
authors.forename: Teruaki
authors.initials: T
authors.lastname: Nakatsuji
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Sonobe
authors.forename: Katsumi
authors.initials: K
authors.lastname: Aida
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001681:Biological Assay; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D007447:Invertebrate Hormones; D010455:Peptides; D011994:Recombinant Proteins; C012445:molt-inhibiting hormone