Nucleotide sequence analysis of nucleocapsid protein gene of canine distemper virus isolates in Thailand.
The C-terminal part of the nucleocapsid protein gene of 13 canine distemper virus (CDV) isolates from Thailand, were analyzed. The nucleotide sequences were assigned to two clusters; cluster A exhibited a high degree of homology with the vaccine strain Onderstepoort, 99.10 and 97.61%, respectively, in the two isolates examined. Cluster B appeared closely related to virulent strains registered in the GeneBank database and to the virulent reference strain (A75/17); a total of 11 samples were analyzed, with 94.63-99.10% homology at the same position. The deduced amino acid sequences correlated with the two-nucleotide sequence clusters. However, there was no association among the CDV groups with histories of vaccination, sex, ages, clinical findings and evidence of viral antigen in tissues.
Keawcharoen
J
J
Division of Virology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330 Thailand.
Theamboonlers
A
A
Jantaradsamee
P
P
Rungsipipat
A
A
Poovorawan
Y
Y
Oraveerakul
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004216:Distemper / Q000821:virology*; D004217:Distemper Virus, Canine / Q000235:genetics* / Q000302:isolation & purification; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013785:Thailand; D014765:Viral Vaccines / Q000235:genetics; D014774:Virulence
D004279:DNA, Viral; D019590:Nucleocapsid Proteins; D014765:Viral Vaccines