Isolation and structural identification of diarabinosyl 8-O-4-dehydrodiferulate from maize bran insoluble fibre.
The first saccharide ester of a dehydrodiferulic acid (DFA) other than 5-5-DFA has been isolated from maize bran insoluble fibre after acidic hydrolysis and fractionation by gel chromatography and semi-preparative RP-HPLC. HPLC-MS along with 1D, 2D and 3D NMR spectra provided the requisite structural evidence that it is the di-5-O-l-arabinosyl ester of 8-O-4-DFA. Although a range of DFAs have been well authenticated as components released from the cell walls of grasses, the only structural evidence for a DFA attached to polysaccharides had been from 5-5-DFA. The isolation of the 8-O-4-ester demonstrates that polysaccharides in maize cell walls, and presumably in all grasses, are cross-linked through dehydrodiferulates other than 5-5-dehydrodiferulate.
Allerdings
Ella
E
Department of Food Chemistry, Institute of Biochemistry and Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Ralph
John
J
Schatz
Paul F
PF
Gniechwitz
Diana
D
Steinhart
Hans
H
Bunzel
Mirko
M
D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry* / Q000302:isolation & purification; D004043:Dietary Fiber / Q000032:analysis; D004187:Disaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D003313:Zea mays / Q000737:chemistry*
D003373:Coumaric Acids; D004043:Dietary Fiber; D004187:Disaccharides; C498109:diarabinosyl 8-O-4-dehydrodiferulate