[Antitumor effect of immunizations with fusions of dendritic and hepatocellular carcinoma cells in mice].
OBJECTIVE
To investigate the effects of immunization with fusions of dendritic cells and H22 cells on tumor-bearing mice and their possible mechanisms.


METHODS
Fusion cells of DC and H22 cells were prepared with polyethylene glycol (PEG). Expression of MHC and costimulatory molecules by dendritomas were determined by FACs. To study the antitumor immune preventative and therapeutic effects, fusions were subcutaneously injected into tumor-bearing mice. The cytotoxic T lymphocyte (CTL) activity was determined by LDH method, the expression of TNF-a and IFN-g in tumors were assayed by RT-PCR.


RESULTS
The data showed that the hybridomas of DC and H22 cells acquired both DC and H22 cell phenotypes. Immunization of BALB/C mice with DC/H22 fusions induced potent CTL activity (mean CTL activity=0.624+/-0.024, compared with DC + H22, DC, H22 groups, F = 65.46) and a protective immunity against a high dose of H22 tumor challenge. After treatment with hybridomas, the survival time of tumor-bearing mice was greatly extended (x2=18.45). The expression levels of TNF-a and IFN-g mRNA were remarkably increased (TNF-a, F = 47.84; IFN-g, F = 37.23).


CONCLUSIONS
The hybridomas of DC and H22 cells could induce effective antitumor immune responses and may have a useful potential in prevention and management of the recurrences and metastases of HCC.
Zhang
Hao
H
The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
Zheng
Shu-shen
SS
Jiang
Guo-ping
GP
Zhou
Lin
L
Xie
Hai-yang
HY
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology; D002459:Cell Fusion; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006825:Hybridomas; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D014611:Vaccination
D019496:Cancer Vaccines; D014409:Tumor Necrosis Factor-alpha; D011092:Polyethylene Glycols; D007371:Interferon-gamma