[Cloning and sequence analysis of variable region gene of anti-idiotype monoclonal antibody against Vibrio alginolyticus].
OBJECTIVE
To clone and sequence V(H) and V(L) genes of anti-idiotype monoclonal antibody (mAb) against vibrio alginolyticus.


METHODS
Total RNA was extracted from hybridoma cell AL1 secreting mAb against vibrio alginolyticus and cDNA was amplified by RT-PCR. Then the cDNA was inserted into PMD18-T vector and its sequence was analyzed.


RESULTS
The V(H) gene contained 369 bp and encoded 123 amino acid residues; the V(L) gene contained 339 bp and encoded 113 amino acid residues. There were four FRs, three CDRs and two characteristic cysteine residues in the V(H) and V(L) genes, respectively.


CONCLUSIONS
The successful cloning of the V(H) and V(L) genes of anti-idiotype mAb against vibrio alginolyticus provides a sound basis for construction of gene-engineering vaccine of the anti-idiotype mAb against vibrio alginolyticus.
Fu
Jian-Fang
JF
Department of Histology and Embryology, Fourth Military Medical University, Xi'an 710032, China. jianff@fmmu.edu.cn
Huang
Wei-Quan
WQ
Xia
Yong-Juan
YJ
Yang
An-Gang
AG
D000595:Amino Acid Sequence; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry* / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D005818:Genetic Engineering; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D044149:Vibrio alginolyticus / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region