[Construction of an eukaryotic expression plasmid for short peptides used in DNA immunization].
OBJECTIVE
To construct a plasmid which efficiently express short peptides in DNA immunization.


METHODS
The plasmid containing peptide-expressing cassette (PEC) was constructed and its effect in DNA immunization was investigated, using a DHBV B cell epitope as the short peptide. The peptide in vitro was detected by DOT-EIA. The BALB/c mice were immunized with the empty plasmid or the recombinant plasmid, and the specific antibodies against the epitope in the sera of the mice were determined by ELISA.


RESULTS
The plasmid containing peptide-expressing cassette (PEC) was successfully constructed. Recombinant epitope-based plasmid could efficiently express the short peptide in vitro and induce immune response against it in DNA immunization.


CONCLUSIONS
The constructed vector provides highly efficient short peptide expression in DNA immunization.
Xu
Huan-Bin
HB
Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, China.
Xu
Wei
W
Chu
Yi-Wei
YW
Wang
Ying
Y
Zhang
Rui-Hua
RH
Xiong
Si-Dong
SD
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005057:Eukaryotic Cells / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D015611:Hepatitis B Virus, Duck / Q000276:immunology; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism
D010455:Peptides; D019444:Vaccines, DNA; D004262:DNA Restriction Enzymes