Effects of advanced glycation end products on hyaluronan photolysis: a new mechanism of diabetic vitreopathy.
OBJECTIVE
To test the effects of advanced glycation end products (AGEs), which are increased in vitreous of diabetic patients, on photolysis of hyaluronan.


METHODS
Pullulan standards were used as molecular weight (MW) markers to obtain a calibration curve. 0.02% hyaluronan solutions were divided into AGE-added and AGE-free samples; each sample was irradiated using a xenon lamp or kept in the dark. Retention time (RT) was measured for each sample using high-performance liquid chromatography.


RESULTS
RTs and logarithm of MW of pullulan standards were negatively correlated. In hyaluronan samples exposed to light, RT increased significantly for both AGE-added and AGE-free samples compared with samples kept in the dark. RT in AGE-added samples was greater by 3% than that in AGE-free samples (p = 0.02).


CONCLUSIONS
Exposure to light decreases MW of hyaluronan; addition of AGEs promotes this change. The photosensitizer activity of AGEs may be associated with accelerated depolymerization of hyaluronan in diabetic patients.
Katsumura
Chihiro
C
Department of Ophthalmology, Osaka Medical College, Takatsuki, Osaka, Japan.
Sugiyama
Tetsuya
T
Nakamura
Kimitoshi
K
Obayashi
Hiroshi
H
Hasegawa
Goji
G
Oku
Hidehiro
H
Ikeda
Tsunehiko
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003930:Diabetic Retinopathy / Q000378:metabolism*; D005128:Eye Diseases / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism* / Q000528:radiation effects*; D008970:Molecular Weight; D010782:Photolysis; D014822:Vitreous Body / Q000378:metabolism*
D017127:Glycation End Products, Advanced; D006820:Hyaluronic Acid