Characterisation of iota-carrageenans oligosaccharides with high-performance liquid chromatography coupled with evaporative light scattering detection.
Enzymatically digested oligo-iota-carrageenans were separated with liquid chromatography, coupled to evaporative light scattering detection. As expected, compared to oligo-kappa-carrageenans, the additional sulphate group in the neocarrabiose unit of iota-carrageenans significantly modified the separation mechanisms on ion-exchange chromatography, porous graphitic carbon and ion-pair chromatography. The oligomers were then isolated and characterised off-line with electrospray ionisation mass spectrometry in positive-ion mode. The tetrasaccharide, hexasaccharide and octasaccharide that were identified were associated with protonated heptylamine molecules whose number depended on the number of sulphate groups.
Antonopoulos
A
A
Institut de Chimie Organique et Analytique (ICOA), UMR CNRS 6005, Université d'Orléans, BP 6759, 45067 Orléans Cedex 2, France.
Favetta
P
P
Lafosse
M
M
Helbert
W
W
D000837:Anion Exchange Resins; D002351:Carrageenan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D009844:Oligosaccharides / Q000737:chemistry*; D012542:Scattering, Radiation; D021241:Spectrometry, Mass, Electrospray Ionization
D000837:Anion Exchange Resins; D009844:Oligosaccharides; D002351:Carrageenan