title: In vitro and in vivo translational efficiencies of the 5' untranslated region from eight genotype 2 bovine viral diarrhea virus field isolates.
abstract: We determined the in vitro and in vivo translational efficiency mediated by the internal ribosomal entry site (IRES) from eight BVDV2 field isolates varying in virulence using a bicistronic reporter vector in rabbit reticulocyte lysates (RRL), and in primate and bovine cell lines. Using a T7-promoter system, the high virulence isolates had greater translational efficiencies in bovine lymphocytes (BL-3 cells), than did the low virulence isolates. The low virulence isolates translated with greater efficiencies than the high virulence isolates in RRL, African green monkey kidney (CV-1) and bovine turbinate (BT) cells. Our results demonstrate that despite a high degree of sequence identity in the 5' untranslated region (UTR), subtle differences in the primary and secondary structures, as well as differences in cell lines, influence translational efficiencies.
authors.affiliation: Department of Veterinary and Biomedical Sciences, Veterinary Basic Science, University of Nebraska, East Campus Loop and Fair Street, VBS 210, Lincoln, NE 68583-0905, USA.
authors.forename: Christina L
authors.initials: CL
authors.lastname: Topliff
authors.forename: Seung K
authors.initials: SK
authors.lastname: Chon
authors.forename: Ruben O
authors.initials: RO
authors.lastname: Donis
authors.forename: Kent M
authors.initials: KM
authors.lastname: Eskridge
authors.forename: Clayton L
authors.initials: CL
authors.lastname: Kelling
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology; D002417:Cattle; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000472:pathogenicity; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D014774:Virulence
chemical_list: D020121:5' Untranslated Regions; D004279:DNA, Viral