[Molecular mutations of rpoB gene of multidrug resistant Mycobacterium tuberculosis isolates from China].
To characterize rpoB mutations of multidrug resistant Mycobacterium tuberculosis clinical isolates from China, mutations in the 81 bp rifampin resistance determining region (RRDR) and mutation V176F locating in the beginning of the rpoB gene were analyzed by DNA sequencing. Eighty six Mycobacterium tuberculosis clinical isolates, including 72 rifampin resistant strains and 14 rifampin sensitive strains were sequenced. Six five mutations of 22 distinct kinds, 21 point mutations and one insertion, were observed in 65 of 72 resistant isolates. The most frequent mutations were in codon 531 (41%), 526 (40%), and 516 (4%). Mutations were not found in seven (10%) of the resistant isolates. Six new alleles within the RRDR, along with five novel mutations outside the RRDR, are reported. None of isolates contained the V176 mutation.
Yue
Jun
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, College of Life Science, Fudan University, Shanghai 200433, China. yuejunnan@yahoo.com.cn
Zeng
Er-Liang
EL
Xie
Jian-Ping
JP
Li
Yao
Y
Liang
Li
L
Wang
Hong-Hai
HH
D001426:Bacterial Proteins / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*
C119094:ARR-2 protein, Bacteria; D001426:Bacterial Proteins; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit