title: TRPV1b, a functional human vanilloid receptor splice variant.
abstract: Transient receptor potential (TRP) genes encode a family of related ion-channel subunits. This family consists of cation-selective, calcium-permeable channels that include a group of vanilloid receptor channels (TRPV) implicated in pain and inflammation. These channels are activated by diverse stimuli, including capsaicin, lipids, membrane deformation, heat, and protons. Six members of the TRPV family have been identified that differ predominantly in their activation properties. However, in neurons, TRPV channels do not account for the observed diversity of responses to activators. By probing human and rat brain cDNA libraries to identify TRPV subunits, we identified a novel human TRPV1 RNA splice variant, TRPV1b, which forms functional ion channels that are activated by temperature (threshold, approximately 47 degrees C), but not by capsaicin or protons. Channels with similar activation properties were found in trigeminal ganglion neurons, suggesting that TRPV1b receptors are expressed in these cells and contribute to thermal nociception.
authors.affiliation: Box 3209, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.forename: Dorian
authors.initials: D
authors.lastname: Henderson
authors.forename: Lieju
authors.initials: L
authors.lastname: Liu
authors.forename: Peter H
authors.initials: PH
authors.lastname: Reinhart
authors.forename: Sidney A
authors.initials: SA
authors.lastname: Simon
mesh_terms: D000595:Amino Acid Sequence; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003001:Cloning, Molecular; D006801:Humans; D007473:Ion Channels / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D012430:Ruthenium Red / Q000494:pharmacology; D013329:Structure-Activity Relationship; D050916:TRPV Cation Channels; D013696:Temperature; D012668:Trigeminal Ganglion / Q000737:chemistry / Q000502:physiology
chemical_list: D007473:Ion Channels; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D012430:Ruthenium Red; C071423:capsazepine; D002211:Capsaicin