Meconium induced IL-8 production and intratracheal albumin alleviated lung injury in newborn pigs.
We have recently shown that albumin added to meconium before intratracheal instillation in newborn pigs limits detrimental effect on the lungs and reduces increase of IL-8. The aim of this study was to test the effect of albumin instillation as rescue treatment in meconium aspiration syndrome (MAS). MAS was induced in hypoxic piglets by lung instillation of meconium (MAS I = 675 mg/kg, n=12; MAS II=540 mg/kg, n=14). Morbidity and mortality differed (MAS I, dead=7/12; MAS II, dead=5/14). MAS groups were randomized to postmeconium instillation of either bovine albumin (30%, 1.4 mL/kg; MAS I, n=6; MAS II, n=7) or isotonic saline (9 mg/mL, 1.4 mL/kg; MAS I, n=6; MAS II, n=7). The controls (n=4) were tested by sequential instillation of saline (9 mg/mL, 5 mL/kg) and albumin (30%, 1.4 mL/kg). Lung function and gas exchange deteriorated significantly after instillation of meconium [oxygenation index (OI): MAS I, +814%; MAS II, +386%; ventilation index (VI): MAS I, +256%; MAS II, +162%; compliance: MAS I, -53%; MAS II, -44%]. Increases of tracheal IL-8 correlated to deterioration of lung function were 10- (MAS I) and 5-fold (MAS II) (p <0.001). Lung compliance was higher in albumin instillation versus saline instillation (MAS I, p=0.008; MAS II, p=0.002). Albumin did not influence intergroup differences in IL-8, hemodynamics, OI, or VI. MAS-induced IL-8 increases correlated with deterioration of lung function (OI, VI, and compliance). Rescue treatment with albumin in meconium aspiration improved lung compliance in piglets and may represent a new therapeutic approach to MAS.
Lindenskov
Paal H H
PH
Department of Pediatric Research, Institute for Surgical Research, Rikshospitalet University Hospital, N-0027 Oslo, Norway. p.h.h.lindenskov@klinmed.uio.no
Castellheim
Albert
A
Aamodt
Geir
G
Saugstad
Ola D
OD
D000418:Albumins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000831:Animals, Newborn; D001774:Blood Chemical Analysis; D002417:Cattle; D006439:Hemodynamics; D006801:Humans; D007231:Infant, Newborn; D016209:Interleukin-8 / Q000276:immunology* / Q000378:metabolism*; D008168:Lung / Q000187:drug effects; D055370:Lung Injury; D008470:Meconium / Q000276:immunology*; D008471:Meconium Aspiration Syndrome / Q000188:drug therapy* / Q000276:immunology / Q000401:mortality; D013223:Statistics as Topic; D013552:Swine
D000418:Albumins; D016209:Interleukin-8