Identification of 4-methylspinaceamine--a Pictet-Spengler condensation reaction product of histamine with acetaldehyde--in human urine.
This study reports the first identification of 4-methylspinaceamine (4-MSPA)-a Pictet Spengler condensation reaction product of histamine with acetaldehyde-in human urine. 4-MSPA was identified and quantified as follows: the target compound was partially purified by solvent extraction from a urine sample spiked with N-methylpiperazine (N-MP) as an internal standard, then derivatized to a naphthylthiourea derivative with 1-naphthylisothiocyanate (NITC) and finally analyzed by HPLC. For verification, 4-MSPA was also analyzed by ion spray-mass spectrometry (IS-MS), using 4-MSPA-d4 as an internal standard. The amount of 4-MSPA in human urine varied between individuals and from day-to-day, ranging from undetectable to 0.80 nmol/mL.
Ohya
Takeshi
T
Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyakidai, Sakado, Saitama 350-0295, Japan. tohya@josai.ac.jp
Niitsu
Masaru
M
D000079:Acetaldehyde / Q000737:chemistry*; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006632:Histamine / Q000737:chemistry*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry / Q000652:urine*; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D011725:Pyridines / Q000737:chemistry / Q000652:urine*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization
C496999:4-methylspinaceamine; D007093:Imidazoles; D011725:Pyridines; D006632:Histamine; D000079:Acetaldehyde