title: The effect of O-glcnac glycosylation of rat liver nucleoproteins on their acute phase-dependent binding ability to the hormone responsive element of the haptoglobin gene.
abstract: We examined whether the transcriptional activation of the rat haptoglobin (Hp) gene during the acute phase (AP) response reflects the O-linked N-acetylglucosamine (O-GlcNAc) status of liver nucleoproteins (NPs) and their binding for the hormone responsive element (HRE). After deglycosylation with N-acetylglucosaminidase of the O-GlcNAc glycoproteins obtained by WGA, affinity chromatography and South-Western analysis, it was observed that only increased HRE binding ability of p64/p70 in control and p51 obtained from turpentine-treated rats can be directly attributed to the presence of O-GlcNAc residues. Therefore, expression of the rat Hp gene could be controlled by this modification of certain trans-acting NPs.
authors.affiliation: Department of Molecular Biology, Institute of Biological Research, Belgrade, Serbia and Montenegro. iligri@ibiss.bg.ac.yu
authors.forename: I
authors.initials: I
authors.lastname: Grigorov
authors.forename: I
authors.initials: I
authors.lastname: Cvetković
authors.forename: T
authors.initials: T
authors.lastname: Milosavljević
authors.forename: A
authors.initials: A
authors.lastname: Uskoković
authors.forename: D
authors.initials: D
authors.lastname: Bogojević
authors.forename: S
authors.initials: S
authors.lastname: Ivanović-Matić
authors.forename: G
authors.initials: G
authors.lastname: Poznanović
authors.forename: M
authors.initials: M
authors.lastname: Petrović
mesh_terms: D000117:Acetylglucosamine / Q000235:genetics / Q000378:metabolism*; D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D006031:Glycosylation; D006242:Haptoglobins / Q000235:genetics* / Q000378:metabolism*; D006728:Hormones / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D020218:Response Elements / Q000187:drug effects / Q000235:genetics; D015533:Transcriptional Activation / Q000502:physiology
chemical_list: D000209:Acute-Phase Proteins; D006242:Haptoglobins; D006728:Hormones; D009698:Nucleoproteins; D000117:Acetylglucosamine