[Study on inhibitory effect of CpG-ODN on HBV replication in vitro].
OBJECTIVE
To study the inhibitory effect of synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG dinucleotides (CpG-ODN) on hepatitis B virus(HBV) replication in vitro.


METHODS
CpG-ODN was used to stimulate peripheral blood mononuclear cells(PBMCs). The IFN-alpha and IFN-gamma levels in the culture supernatant of stimulated PBMCs were measured by ELISA. The culture supernatant was added to HBV-transfected HepG2.2.15 cells, and after 2, 4, 6 and 8 days, HBsAg, HBeAg and HBV DNA levels in culture supernatant of 2.2.15 cells were detected by ELISA and quantitative PCR respectively.


RESULTS
The CpG-ODN alone could not inhibit HBV replication, whereas the culture supernatant of PMBCs activated with CpG-ODN could remarkably inhibit the production of HBsAg, HBeAg and HBV DNA by 2.2.15 cells, reaching the maximal inhibitory rates of 90.8%, 31.3% and 32.2%, respectively on the 8th day.


CONCLUSIONS
The study indicates that, as a novel immunoregulator, CpG-ODN can inhibit indirectly HBV replication in vitro.
Li
Ning
N
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China. nxli@hotmail.com
Fan
Xue-Gong
XG
Chen
Zhao-Hui
ZH
Zhu
Cai
C
D002460:Cell Line; D018899:CpG Islands / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects / Q000276:immunology; D004279:DNA, Viral / Q000096:biosynthesis; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects* / Q000276:immunology
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D009838:Oligodeoxyribonucleotides