Simultaneous determination of l- and d-lactic acid in plasma by capillary electrophoresis.
A novel method for simultaneous determination of d- and l-lactic acids in plasma was presented by capillary electrophoresis with photodiode array detection at 195nm. The separation was performed in an uncoated fused-silica capillary. The parameters influencing the resolution and the migration time of lactic acids were optimized. When 150mM phosphate-Tris buffer (pH 7.0) consisting of 220mM 2-hydroxypropyl-beta-cyclodextrin and 0.2mM tetradecyltrimethylammonium bromide was utilized as the running buffer, highly effective chiral separation of d- and l-lactic acids was achieved at about 42min at an effective voltage of -25kV. The resolution of lactic acid enantiomers was >/=1.25. The limits of detection of d- and l-lactic acids in standard solution without any pretreatment were 80 and 50muM (S/N=3), respectively. Sample pretreatment was preceded by protein-removal procedure with acetonitrile. With a pre-concentration procedure by 10 times, the limits of detection of d- and l-lactic acids were 20 and 15muM (S/N=10), respectively. The satisfactory analytical performance of the proposed method was validated.
Tan
Li
L
Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, China; Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, China.
Wang
Yu
Y
Liu
Xiaoquan
X
Ju
Huangxian
H
Li
Jieshou
J
D000818:Animals; D002021:Buffers; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D051381:Rats; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D002021:Buffers; D004573:Electrolytes; D019344:Lactic Acid