Endogenous protein kinase-C activity and phosphorylated proteins in messenger ribonucleoprotein complexes of developing embryos of alfalfa.
Developing somatic and zygotic embryos of alfalfa (Medicago sativa L.) exhibited endogenous protein kinase activity and protein acceptors of phosphate groups using both cell-free translational extracts and oligo(dT)-cellulose-column-purified mRNPs. The cell-free-translation extracts from pre-cotyledonary-stage somatic embryos had approximately 50- and 100-fold more protein kinase activity than cotyledonary-stage somatic and zygotic embryos. Several polypeptides were phosphorylated; some of them were unique to the early stage and some to the late-stage developing embryos. A 65 kDa protein was phosphorylated heavily in pre-cotyledonary-stage somatic embryos. This phosphorylated protein was comprised of three main components, two of which were phosphorylated heavily. Heat-shock treated-embryos lost their exitant kinase activity and at the same time another form of protein kinase activity was activated which phosphorylated a novel 28 kDa protein. Endogenous protein kinase activity was also observed within the mRNPs of polysomal and non-polysomal fractions of developing embryos, and this phosphorylated only 65, 43 and 30 kDa proteins within these fractions. A 30 kDa protein from the pre-cotyledonary-stage somatic embryos showed a higher affinity for accepting phosphate groups than the proteins from cotyledonary-stage somatic or zygotic embryos. The activity of protein kinase was largely c-AMP-independent, but was dependent on Ca2+, phospholipid and phorbol ester. The enzyme belongs to the protein kinase-C family; the 65 kDa protein cross-reacts with antibodies made against protein kinase-C (alpha- and beta-isoforms) and it may be an autophosphorylated protein.
Pramanik
S K
SK
Department of Botany, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. Spramani@jewl.morgan.edu
Williams
A L
AL
Bewley
J Derek
JD
D018548:Cotyledon / Q000201:enzymology / Q000276:immunology; D000242:Cyclic AMP / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology; D000455:Medicago sativa / Q000196:embryology* / Q000201:enzymology* / Q000276:immunology; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D046188:Protein Modification, Translational / Q000502:physiology; D012261:Ribonucleoproteins / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology / Q000276:immunology
D010940:Plant Proteins; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D000242:Cyclic AMP; D011493:Protein Kinase C