Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting.
We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P=0.057, r=0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.
Imade
Godwin E
GE
AIDS Prevention Initiative Nigeria, Jos University Teaching Hospital, Jos, Nigeria. ereimade@yahoo.co.uk
Badung
Bitrus
B
Pam
Sunday
S
Agbaji
Oche
O
Egah
Daniel
D
Sagay
Atiene S
AS
Sankalé
Jean-Louis
JL
Kapiga
Saidi
S
Idoko
John
J
Kanki
Phyllis
P
D000328:Adult; D001331:Automation; D018791:CD4 Lymphocyte Count / Q000191:economics / Q000379:methods*; D005434:Flow Cytometry / Q000191:economics / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000191:economics / Q000379:methods; D009549:Nigeria; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity