[Bioassay of recombinant human platelet factor 4].
OBJECTIVE
To assay the biological activity of recombinant human platelet factor 4 (rhPF4).


METHODS
The bioactivity of rhPF4 was assayed in vitro and in vivo in comparison with native PF4 by using murine and human megakaryocyte culture systems.


RESULTS
rhPF4, like native PF4, had an inhibitory activity on megakaryocytopoiesis in vitro and in vivo in mice. The inhibitory activity could be neutralized by heparin. rhPF4 also had an inhibitory activity on human megakaryocytic leukemia cell line (Meg-01).


CONCLUSIONS
rhPF4 might be applied to clinical practice.
Han
Z
Z
Shanghai BEITE Biotechnology Co. Ltd, Shanghai 200093.
Shen
Q
Q
Han
Z
Z
D000818:Animals; D001681:Biological Assay; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010978:Platelet Factor 4 / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*
D011994:Recombinant Proteins; D010978:Platelet Factor 4; D006493:Heparin