Phototoxic effect of visible light on Porphyromonas gingivalis and Fusobacterium nucleatum: an in vitro study.
The antibacterial effect of visible light irradiation combined with photosensitizers has been reported. The objective of this was to test the effect of visible light irradiation without photosensitizers on the viability of oral microorganisms. Strains of Porphyromonas gingivalis, Fusobacterium nucleatum, Streptococcus mutans and Streptococcus faecalis in suspension or grown on agar were exposed to visible light at wavelengths of 400-500 nm. These wavelengths are used to photopolymerize composite resins widely used for dental restoration. Three photocuring light sources, quartz-tungsten-halogen lamp, light-emitting diode and plasma-arc, at power densities between 260 and 1300 mW/cm2 were used for up to 3 min. Bacterial samples were also exposed to a near-infrared diode laser (wavelength, 830 nm), using identical irradiation parameters for comparison. The results show that blue light sources exert a phototoxic effect on P. gingivalis and F. nucleatum. The minimal inhibitory dose for P. gingivalis and F. nucleatum was 16-62 J/cm2, a value significantly lower than that for S. mutans and S. faecalis (159-212 J/cm2). Near-infrared diode laser irradiation did not affect any of the bacteria tested. Our results suggest that visible light sources without exogenous photosensitizers have a phototoxic effect mainly on Gram-negative periodontal pathogens.
Feuerstein
Osnat
O
Department of Prosthodontics, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel.
Persman
Nir
N
Weiss
Ervin I
EI
D003470:Culture Media; D016967:Fusobacterium nucleatum / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D008027:Light; D017319:Photosensitizing Agents / Q000633:toxicity*; D016966:Porphyromonas gingivalis / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D013696:Temperature
D003470:Culture Media; D017319:Photosensitizing Agents