title: Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques.
abstract: Hypoglycemic effect of Gentiana olivieri Griseb. (Gentianaceae) flowering herbs on oral administration were studied using in vivo models in normal, glucose-hyperglycemic and streptozotocin-induced diabetic rats. Through in vivo bioassay-guided fractionation processes isoorientin, a known C-glycosylflavone, was isolated from the ethylacetate fraction by silica gel column chromatography as the main active ingredient from the plant. Isoorientin exhibited significant hypoglycemic and antihyperlipidemic effects at 15 mg/kg b.w.dose. Isoorientin concentration of the extracts and fractions were determined by HPLC in order to establish a correlation between the hypoglycaemic activity.
authors.affiliation: Gazi University, Faculty of Pharmacy, Etiler 06330 Ankara, Turkey.
authors.forename: Ekrem
authors.initials: E
authors.lastname: Sezik
authors.forename: Mustafa
authors.initials: M
authors.lastname: Aslan
authors.forename: Erdem
authors.initials: E
authors.lastname: Yesilada
authors.forename: Shigeru
authors.initials: S
authors.lastname: Ito
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D030012:Gentiana / Q000737:chemistry*; D006943:Hyperglycemia / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D007928:Lethal Dose 50; D047311:Luteolin / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D008297:Male; D051379:Mice; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D012997:Solvents; D014280:Triglycerides; D002784:Cholesterol; C057912:homoorientin; D047311:Luteolin