title: Micropatterning proteins and cells on polylactic acid and poly(lactide-co-glycolide).
abstract: Techniques for micropatterning proteins and cells on biomaterials are important in tissue engineering applications. Here, we present a method for patterning proteins and cells on poly(lactic acid) (PLA) and poly(lactide-co-glycolide) (PLGA) substrates that are routinely used as scaffolds in engineering tissues. Poly(oligoethyleneglycol methacrylate) (poly-OEGMA) or poly(oligoethyleneglycol methacrylate-co-methacrylic acid) (poly(OEGMA-co-MA)) was microcontact printed onto substrates to create cell resistant areas. Proteins adsorbed onto the unprinted regions whereas the polymer printed regions effectively repel non-specific protein adsorption. NIH 3T3 fibroblasts remain confined within the patterns on the PLGA and PLA films for up to 2 weeks and aligned their actin cytoskeleton along the line patterns. In comparison to unpatterned cells, fibroblasts confined within line-shaped patterns show fewer actin filaments. This method for controlling the spatial morphology and distribution of cells on synthetic biomaterials could have significant applications in tissue engineering.
authors.affiliation: Department of Chemical and Materials Engineering, University of Cincinnati, 497 Rhodes Hall, Cincinnati, OH 45221, USA.
authors.forename: Chien-Chi
authors.initials: CC
authors.lastname: Lin
authors.forename: Carlos C
authors.initials: CC
authors.lastname: Co
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Ho
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D016764:Cell Polarity; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C033616:poly(lactide)