SigB-dependent in vitro transcription of prfA and some newly identified genes of Listeria monocytogenes whose expression is affected by PrfA in vivo.
Recent studies have identified several new genes in Listeria monocytogenes which are positively or negatively affected by PrfA and grouped into three classes (E. Milohanic et al., Mol. Microbiol. 47:1613-1625, 2003). In vitro transcription performed with promoters of some class III genes showed strict SigB-dependent but PrfA-independent transcription initiation. Transcription starting at the prfA promoter PprfA2 was also optimal with SigB-loaded RNA polymerase, suggesting a direct link between SigB- and PrfA-dependent gene expression.
Rauch
Marcus
M
Biocenter (Microbiology), University of Würzburg, 97074 Würzburg, Germany.
Luo
Qin
Q
Müller-Altrock
Stefanie
S
Goebel
Werner
W
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000502:physiology; D010454:Peptide Termination Factors; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D010454:Peptide Termination Factors; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D015534:Trans-Activators