title: Phosphorylation-dependent translocation of sphingosine kinase to the plasma membrane drives its oncogenic signalling.
abstract: Sphingosine kinase (SK) 1 catalyzes the formation of the bioactive lipid sphingosine 1-phosphate, and has been implicated in several biological processes in mammalian cells, including enhanced proliferation, inhibition of apoptosis, and oncogenesis. Human SK (hSK) 1 possesses high instrinsic catalytic activity which can be further increased by a diverse array of cellular agonists. We have shown previously that this activation occurs as a direct consequence of extracellular signal-regulated kinase 1/2-mediated phosphorylation at Ser225, which not only increases catalytic activity, but is also necessary for agonist-induced translocation of hSK1 to the plasma membrane. In this study, we report that the oncogenic effects of overexpressed hSK1 are blocked by mutation of the phosphorylation site despite the phosphorylation-deficient form of the enzyme retaining full instrinsic catalytic activity. This indicates that oncogenic signaling by hSK1 relies on a phosphorylation-dependent function beyond increasing enzyme activity. We demonstrate, through constitutive localization of the phosphorylation-deficient form of hSK1 to the plasma membrane, that hSK1 translocation is the key effect of phosphorylation in oncogenic signaling by this enzyme. Thus, phosphorylation of hSK1 is essential for oncogenic signaling, and is brought about through phosphorylation-induced translocation of hSK1 to the plasma membrane, rather than from enhanced catalytic activity of this enzyme.
authors.affiliation: Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide SA 5000, Australia. stuart.pitson@imvs.sa.gov.au
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Pitson
authors.forename: Pu
authors.initials: P
authors.lastname: Xia
authors.forename: Tamara M
authors.initials: TM
authors.lastname: Leclercq
authors.forename: Paul A B
authors.initials: PA
authors.lastname: Moretti
authors.forename: Julia R
authors.initials: JR
authors.lastname: Zebol
authors.forename: Helen E
authors.initials: HE
authors.lastname: Lynn
authors.forename: Binks W
authors.initials: BW
authors.lastname: Wattenberg
authors.forename: Mathew A
authors.initials: MA
authors.lastname: Vadas
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D006801:Humans; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D014162:Transfection; D000099157:Sphingosine Kinase
chemical_list: D017931:DNA Primers; D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase