title: Detection of human influenza A viruses by loop-mediated isothermal amplification.
abstract: Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test.
authors.affiliation: Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR. llmpoon@hkucc.hku.hk
authors.forename: Leo L M
authors.initials: LL
authors.lastname: Poon
authors.forename: Cynthia S W
authors.initials: CS
authors.lastname: Leung
authors.forename: Kwok H
authors.initials: KH
authors.lastname: Chan
authors.forename: Jack H C
authors.initials: JH
authors.lastname: Lee
authors.forename: Kwok Y
authors.initials: KY
authors.lastname: Yuen
authors.forename: Yi
authors.initials: Y
authors.lastname: Guan
authors.forename: Joseph S M
authors.initials: JS
authors.lastname: Peiris
mesh_terms: D000199:Actins / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral