title: Repeated immunization induces the increase in fucose content on antigen-specific IgG N-linked oligosaccharides.
abstract: OBJECTIVE
  In order to investigate whether repeated immunization induces changes in IgG glycosylation, we analyzed the composition of oligosaccharides on antigen-specific IgGs obtained from mice that received different amounts of immunization boosts.
  
  
  METHODS
  Three groups of mice were immunized with ovalbumin (OVA) and boosted once, twice, or three times, respectively, with an interval of 1 week. The patterns of oligosaccharides present in anti-OVA specific IgGs were analyzed using lectin-enzyme-linked immunosorbent assay (ELISA) and lectin-blot.
  
  
  RESULTS
  The repeated injection of OVA induced both the production of specific IgGs and an increase of fucose content in N-linked oligosaccharides of the IgGs. The lowest IgG fucosylation was observed in mice boosted once, whereas the highest fucosylation rate was observed in mice boosted three times. ELISA assay demonstrated that there was a positive relationship between the fucose content and amount of immunization boosts.
  
  
  CONCLUSIONS
  IgG fucosylation increases during repeated immunization with ovalbumin. The alteration of IgG fucosylation may have important biological significance.
authors.affiliation: Department of Biochemistry, School of Medicine, Akita University, 1-1-1 Hondo, Akita 010-8543, Japan.
authors.forename: Naxin
authors.initials: N
authors.lastname: Guo
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Masuda
authors.forename: Masami
authors.initials: M
authors.lastname: Kawagoe
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Ueno
authors.forename: Takashi
authors.initials: T
authors.lastname: Kameda
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005643:Fucose / Q000032:analysis*; D006031:Glycosylation; D007114:Immunization; D007074:Immunoglobulin G / Q000737:chemistry*; D037102:Lectins / Q000032:analysis; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D010047:Ovalbumin / Q000008:administration & dosage
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D037102:Lectins; D009844:Oligosaccharides; D005643:Fucose; D010047:Ovalbumin