Effects of buoyancy-driven convection on nucleation and growth of protein crystals.
Protein crystallization has been studied in presence or absence of buoyancy-driven convection. Gravity-driven flow was created, or suppressed, in protein solutions by means of vertically directed density gradients that were caused by generating suitable temperature gradients. The presence of enhanced mixing was demonstrated directly by experiments with crustacyanin, a blue-colored protein, and other materials. Combined with the vertical tube position the enhanced convection has two main effects. First, it reduces the number of nucleated hen-egg-white lysozyme (HEWL) crystals, as compared with those in a horizontal capillary. By enabling better nutrition from the protein in the solution, convection results in growth of fewer larger HEWL crystals. Second, we observe that due to convection, trypsin crystals grow faster. Suppression of convection, achieved by decreasing solution density upward in the capillary, can to some extent mimic conditions of growth in microgravity. Thus, impurity supply, which may have a detrimental effect on crystal quality, was avoided.
Nanev
Christo N
CN
Institute of Physical Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria. nanev@ipchp.ipc.bas.bg
Penkova
Anita
A
Chayen
Naomi
N
D000818:Animals; D002352:Carrier Proteins; D002645:Chickens; D018481:Convection; D003460:Crystallization; D003461:Crystallography; D004529:Egg White; D006112:Gravitation; D009113:Muramidase / Q000737:chemistry; D010179:Pancreas / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*; D013696:Temperature; D014357:Trypsin / Q000737:chemistry; D014893:Weightlessness
D002352:Carrier Proteins; D011506:Proteins; C018494:crustacyanins; C421864:hen egg lysozyme; D009113:Muramidase; D014357:Trypsin