title: Upregulation of DNA repair genes in active cirrhosis associated with hepatocellular carcinoma.
abstract: Phenotypic changes in injured livers involve complex network of genes whose interplays may lead to fibrosis and cirrhosis, a major risk of hepatocellular carcinoma. Gene expression profiles in fibrotic livers were analyzed by using cDNA microarray, hierarchical clustering and gene ontology. Analyses of a major cluster of upregulated genes in cirrhosis identified a new set of genes involved in DNA repair and damage. The upregulation of DNA repair genes was confirmed by real-time quantitative polymerase chain reaction and associated with necroinflammatory activity (P<0.001). Increased DNA repair activity in cirrhosis with inflammatory activity may reflect increased DNA damages as a consequence of chronic liver injury.
authors.affiliation: INSERM U620, Détoxication et Réparation Tissulaire, Facultés de Médecine et Pharmacie, Université de Rennes I, 2 Av. Léon Bernard, 35043 Rennes Cedex, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Zindy
authors.forename: Lise
authors.initials: L
authors.lastname: Andrieux
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnier
authors.forename: Orlando
authors.initials: O
authors.lastname: Musso
authors.forename: Sophie
authors.initials: S
authors.lastname: Langouët
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Campion
authors.forename: Bruno
authors.initials: B
authors.lastname: Turlin
authors.forename: Bruno
authors.initials: B
authors.lastname: Clément
authors.forename: Nathalie
authors.initials: N
authors.lastname: Théret
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004720:Endonucleases / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000276:immunology / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008099:Liver / Q000276:immunology / Q000473:pathology; D008103:Liver Cirrhosis / Q000150:complications* / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation; D051760:Xeroderma Pigmentosum Group A Protein
chemical_list: D004268:DNA-Binding Proteins; C497314:XPA protein, human; D051760:Xeroderma Pigmentosum Group A Protein; C071447:ERCC1 protein, human; D004720:Endonucleases