Cytochrome b6 arginine 214 of Synechococcus sp. PCC 7002, a key residue for quinone-reductase site function and turnover of the cytochrome bf complex.
Quinone-reductase (Q(i)) domains of cyanobacterial/chloroplast cytochrome bf and bacterial/mitochondrial bc complexes differ markedly, and the cytochrome bf Q(i) site mechanism remains largely enigmatic. To investigate the bf Q(i) domain, we constructed the mutation R214H, which substitutes histidine for a conserved arginine in the cytochrome b(6) polypeptide of the cyanobacterium Synechococcus sp. SPCC 7002. At high light intensity, the R214H mutant grew approximately 2.5-fold more slowly than the wild type. Slower growth arose from correspondingly slower overall turnover of the bf complex. Specifically, as shown in single flash turnover experiments of cytochrome b(6) reduction and oxidation, the R214H mutation partially blocked electron transfer to the Q(i) site, mimicking the effect of the Q(i) site inhibitor 2-N-4-hydroxyquinoline-N-oxide. The kinetics of cytochrome b(6) oxidation were largely unaffected by hydrogen-deuterium exchange in the mutant but were slowed considerably in the wild type. This suggests that although protonation events influenced the kinetics of cytochrome b(6) oxidation at the Q(i) site in the wild type, electron flow limited this reaction in the R214H mutant. Redox titration of membranes revealed midpoint potentials (E(m,7)) of the two b hemes similar to those in the wild type. Our data define cytochrome b(6) Arg(214) as a key residue for Q(i) site catalysis and turnover of the cytochrome bf complex. In the recent cytochrome bf structures, Arg(214) lies near the Q(i) pocket and the newly discovered c(i) or x heme. We propose a model for Q(i) site function and a role for Arg(214) in plastoquinone binding.
Nelson
Matthew E
ME
Department of Biology and Microbiology, University of Wisconsin-Oshkosh, Oshkosh, Wisconsin 54901, USA.
Finazzi
Giovanni
G
Wang
Qing Jun
QJ
Middleton-Zarka
Kelly A
KA
Whitmarsh
John
J
Kallas
Toivo
T
D000483:Alleles; D000818:Animals; D001120:Arginine / Q000737:chemistry*; D001665:Binding Sites; D002696:Chlamydomonas / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism; D045346:Cytochrome b6f Complex / Q000737:chemistry*; D045347:Cytochromes b6 / Q000737:chemistry*; D004563:Electrochemistry; D004583:Electrons; D006418:Heme / Q000737:chemistry; D006859:Hydrogen; D007700:Kinetics; D008954:Models, Biological; D008957:Models, Genetic; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D010971:Plastoquinone / Q000737:chemistry; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D011809:Quinones / Q000737:chemistry*; D013053:Spectrophotometry; D046940:Synechococcus / Q000378:metabolism*; D013997:Time Factors
D045347:Cytochromes b6; D011809:Quinones; D006418:Heme; D006859:Hydrogen; D045346:Cytochrome b6f Complex; D001120:Arginine; D010971:Plastoquinone; D010100:Oxygen