title: Anomeric specificity of D-glucose production by rat hepatocytes.
abstract: The anomeric specificity of D-glucose metabolism in intact hepatocytes remains a matter of debate. This issue was further investigated in the present study, which is based on the quantification of the alpha- and beta-anomers of the 13C-enriched isotopomers of D-glucose generated by rat liver cells exposed to either D-[1-13C] fructose or D-[2-13C] fructose in the presence of D2O. The D-[1-13C]glucose/D-[6-13C]glucose paired ratios found in the cells exposed to D-[1-13C] fructose and the D-[2-13C]glucose/D-[5-13C]glucose paired ratios found in the cells exposed to D-[2-13C] fructose yielded a paired beta/alpha ratio averaging (mean +/- S.E.M.) 79.3 +/- 6.1%. In the case of the isotopomers of D-glucose formed by gluconeogenesis, the D-[2-13C]glucose/D-[5-13C]glucose and D-[3-13C]glucose/D-[4-13C]glucose paired ratios found in cells exposed to D-[1-13C] fructose, as well as the D-[1-13C]glucose/D-[6-13C]glucose and D-[3-13C]glucose/D-[4-13C]glucose paired ratios found in cells exposed to D-[2-13C]fructose, yielded an alpha/beta paired ratio averaging 75.0 +/- 5.8%. Last, in the cells exposed to D-[2-13C]fructose, the beta/alpha ratio for the C2-deuterated isotopomers of D-[2-13C]glucose represented 78.9 +/- 3.7% of that for the C5-deuterated isotopomers of D-[5-13C]glucose. The three values representative of the anomeric specificity of D-glucose production by liver cells were not significantly different from one another, with an overall mean value of 76.9 +/- 3.6%. These findings unambiguously document that the anomeric specificity of phosphoglucoisomerase is operative in intact hepatocytes, resulting in a preferential output of the alpha-anomer of 13C-enriched D-glucose under the present experimental conditions.
authors.affiliation: Laboratory of Experimental Hormonology (ULB) and High Resolution NMR Centre (VUB), Brussels Free Universities, Brussels, Belgium. malaisse@ulb.ac.be
authors.forename: W J
authors.initials: WJ
authors.lastname: Malaisse
authors.forename: R
authors.initials: R
authors.lastname: Willem
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002478:Cells, Cultured; D017666:Deuterium Oxide; D005632:Fructose / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity
chemical_list: D002247:Carbon Isotopes; D005636:Fructosephosphates; D005632:Fructose; D019298:Glucose-6-Phosphate; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; D017666:Deuterium Oxide