Effective production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR.
The enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) using Erwinia herbicola cells involves the action of tyrosine phenol-lyase (Tpl, EC 4.1.99.2). Since Tpl is only synthesized under L-tyrosine-induced conditions, the addition of L-tyrosine to the medium is unavoidable when preparing cells (the enzyme source), but severely impedes the pure preparation of the final product L-DOPA. We circumvented this problem by using recombinant E. herbicola cells carrying a mutant transcriptional regulator TyrR, which is capable of activating the tpl promoter in the absence of L-tyrosine.
Koyanagi
Takashi
T
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Katayama
Takane
T
Suzuki
Hideyuki
H
Nakazawa
Hidetsugu
H
Yokozeki
Kenzo
K
Kumagai
Hidehiko
H
D004885:Erwinia / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D007980:Levodopa / Q000096:biosynthesis* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics; D014447:Tyrosine Phenol-Lyase / Q000235:genetics / Q000378:metabolism*
D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; C069256:TyrR protein, E coli; D007980:Levodopa; D014447:Tyrosine Phenol-Lyase