[Polymorphism analysis of the goat growth hormone gene in the 5' regulatory sequence].
The polymorphism of goat growth hormone gene in 5'-region in 274 goats including Lubei white goat, introducted Boer goat, pure bred Boer goat , hybrid generation 1 of LuBei white goat and Boer goat , backcross of hybrid generation 1 and Boer goat were detected by PCR-SSCP with two different pairs of primers. The result showed that in 26-239bp most of the Boer goats and hybrid generation presented as AA genotype,while most LuBei white goats presented as BB genotype. In 225-429bp all breeds presented as more CC genotype. The amplified fragments of AA,BB and CC,DD of the two fragments were cloned and sequenced . The result showed that in 26-239bp AA genotype had one substitution mutation at 60 site (C-->T), and base C losing at 211 site and DD genotype had three substitution mutations at 264 site (T-->C), 292 site (T-->A) and 372 site (C-->T) in 225-429bp. The results above-mentioned first confirmed that there were polymorphisms in 5' region of GH gene.
Li
Mei-Yu
MY
College of Animal Science and Technology, LaiYang Agricultural University, Lai Yang Shan Dong 265200.
Min
Ling-Jiang
LJ
Sun
Guo-Qiang
GQ
Pan
Qing-Jie
QJ
Shen
Wei
W
Wang
Guang-Lian
GL
D024506:5' Flanking Region / Q000235:genetics*; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000145:classification / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
D013006:Growth Hormone