Microarray-based resequencing of multiple Bacillus anthracis isolates.
We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 x 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 x 10(-6)). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.
Zwick
Michael E
ME
Biological Defense Research Directorate, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA. mzwick@genetics.emory.edu
Mcafee
Farrell
F
Cutler
David J
DJ
Read
Timothy D
TD
Ravel
Jacques
J
Bowman
Gregory R
GR
Galloway
Darrell R
DR
Mateczun
Alfred
A
D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D002876:Chromosomes, Bacterial / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011995:Recombination, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000191:economics / Q000379:methods* / Q000592:standards; D013997:Time Factors