title: IgE- and IgE+Ag-mediated mast cell migration in an autocrine/paracrine fashion.
abstract: Mast cells are the major effector cells for immediate hypersensitivity and chronic allergic reactions. These cells accumulate in mucosal tissues of allergic reactions, where immunoglobulin E (IgE) is produced locally. Here we provide evidence that, in addition to antigen that can attract IgE-bound mast cells, the type of IgE molecules that efficiently activate mast cells can promote the migration of mast cells in the absence of antigen. IgE- and IgE+Ag-mediated migration involves an autocrine/paracrine secretion of soluble factors including adenosine, leukotriene B4, and several chemokines. Their secretion depends on 2 tyrosine kinases, Lyn and Syk, and they are agonists of G-protein-coupled receptors and signal through phosphatidylinositol 3-kinase gamma, leading to mast cell migration. In mouse experiments, naive mast cells are attracted to IgE, and IgE-sensitized mast cells are attracted to antigen. Therefore, IgE and antigen are implicated in mast cell accumulation at allergic tissue sites with local high IgE levels.
authors.affiliation: Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 10 355 Science Center Dr, San Diego, CA 92121, USA.
authors.forename: Jiro
authors.initials: J
authors.lastname: Kitaura
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Masaaki
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Shaun
authors.initials: S
authors.lastname: Chung
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Michael
authors.initials: M
authors.lastname: Leitges
authors.forename: Dianqing
authors.initials: D
authors.lastname: Wu
authors.forename: Clifford A
authors.initials: CA
authors.lastname: Lowell
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology / Q000494:pharmacology; D019898:Autocrine Communication / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D018925:Chemokines / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology* / Q000494:pharmacology; D016023:Integrins / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D019899:Paracrine Communication / Q000276:immunology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism
chemical_list: D000941:Antigens; D018925:Chemokines; D016023:Integrins; D043562:Receptors, G-Protein-Coupled; D017455:Receptors, IgE; D007073:Immunoglobulin E; D019869:Phosphatidylinositol 3-Kinases