Isolation, tissue distribution and prokaryotic expression of a novel human X-linked gene LHFPL1.
We report here the cloning and characterization of a novel human lipoma HMGIC fusion partner-like 1 (LHFPL1) gene, isolated from human brain cDNA library, and mapped to Xq23 by browsing the UCSC genomic database. LHFPL1 contains an ORF with length of 660bp, encoding a protein with a signal peptide sequence and three transmembrane regions, and its predicted molecular weight is 23.7kDa which coincides with the result of prokaryotic expression. LHFPL1 protein is postulated to be localized at endoplasmic reticulum. RT-PCR amplification in seventen human tissues revealed that LHFPL1 is expressed widely in all tissues, especially highly in lung, thymus, skeleton muscle, colon and ovary. In addition, it was demonstrated that LHFPL1 is also transcribed in six liver tumor cell lines.
Huang
Chaoqun
C
The Institute of Genetics, Fudan University, Handan Road 220, Shanghai 20043, People's Republic of China.
Guo
Jinhu
J
Liu
Shen
S
Shan
Yuxi
Y
Wu
Shiliang
S
Cai
Yongping
Y
Yu
Long
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D041321:Chromosomes, Human, X; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D016415:Sequence Alignment
C500213:LHFPL1 protein, human; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins