title: Monitoring of polychlorinated biphenyls in Belgian human adipose tissue samples.
abstract: Polychlorinated biphenyls (PCBs) were monitored in Belgian human adipose tissue samples from deceased individuals (n=100). Their mean age was 52, ranging from 2 to 91 years. There were 57 men and 43 women. Other known variables were date of autopsy and place of residence. No information about diet or occupation was available. The seven marker congeners PCB 28, 52, 101, 118, 138, 153 and 180 were analysed in the samples with a GC-MS/MS method validated according to Commission Decision 2002/657/EC. Extracted fat was cleaned-up over a glass column filled with n-hexane, acid silica, deactivated alumina and anhydrous sodium sulfate. The whole procedure was subjected to a rigorous quality control programme with retention times, ion chromatograms and intensity ratios of the monitored product ions as identification criteria. The total PCB concentration ranged between 10 and 1640 ng g-1 fat, with a mean value of 658 ng g-1 fat. In the age groups of 0-9 (n=1), 10-19 (n=4), 20-29 (n=11), 30-39 (n=13), 40-49 (n=15), 50-59 (n=14), 60-69 (n=14), 70-79 (n=20), 80-89 (n=6) and 90-99 (n=2), the mean total PCB concentrations were 10, 134, 253, 445, 557, 687, 807, 962, 959, and 1191 ng g-1 fat, respectively. So, there was an increase of PCB body burden with age. For the male subjects (n=57; mean age of 53) the mean total PCB concentration was 633 ng g-1 fat. For the female subjects (n=43; mean age of 52) it was 690 ng g-1 fat. There was no significant sex-related difference in the concentrations of marker PCBs.
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium. sarah.desaeger@ugent.be
authors.forename: S
authors.initials: S
authors.lastname: De Saeger
authors.forename: H
authors.initials: H
authors.lastname: Sergeant
authors.forename: M
authors.initials: M
authors.lastname: Piette
authors.forename: N
authors.initials: N
authors.lastname: Bruneel
authors.forename: W
authors.initials: W
authors.lastname: Van de Voorde
authors.forename: C
authors.initials: C
authors.lastname: Van Peteghem
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001344:Autopsy; D001530:Belgium; D002648:Child; D002675:Child, Preschool; D004785:Environmental Pollutants / Q000097:blood*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D011078:Polychlorinated Biphenyls / Q000097:blood*; D012016:Reference Values; D012737:Sex Factors
chemical_list: D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls