Determination of resting membrane potential of individual neuroblastoma cells (IMR-32) using a potentiometric dye (TMRM) and confocal microscopy.
The potentiometric dye, Tetramethylrhodamine methyl ester (TMRM) has been extensively used with fluorometry or optical microscopy to evaluate the electric potential across plasma or mitochondrial membranes. We present here a TMRM confocal microscopy-based potential measurement technique. Corrections are introduced to minimize nonspecific dye binding and insensitivity to low background levels. We have used this technique to compare the resting membrane potential of proliferating and differentiated human neuroblastoma cells (IMR-32).
Mao
Chen
C
Cellular Bioengineering Laboratory, Faculty of Engineering, Driftmier Engineering Center, University of Georgia, Athens, Georgia 30602, USA.
Kisaalita
William S
WS
D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005456:Fluorescent Dyes; D006801:Humans; D008564:Membrane Potentials; D018613:Microscopy, Confocal / Q000379:methods; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D012235:Rhodamines
D005456:Fluorescent Dyes; D012235:Rhodamines; C401833:tetramethylrhodamine methyl ester