Effects of major dietary antioxidants on inflammatory markers of RAW 264.7 macrophages.
Oxidative stresses are involved in the process of chronic inflammatory diseases. The objective of this study was to evaluate and compare the anti-inflammatory activity of major dietary antioxidants. Murine RAW264.7 macrophages (4 x 10(6) cells) were incubated in DMEM containing 10% FBS supplemented with 5 to 20 microM of alpha-tocopherol, beta-carotene or quercetin for 24 hrs, and then treated with LPS for 16 hrs. The medium was collected to measure TNF-alpha, IL-6, PGE2 and nitrite. Expressions of cyclooxygenase-2(COX-2) and inducible nitric oxide synthase(iNOS) were determined using the harvested cells. Results indicate that TNF-alpha and IL-6 accumulations were significantly reduced by 5 to 20 microM quercetin treatment, and 20 microM of alpha-tocopherol treatment. Nitrite release was significantly reduced by 5 microM quercetin treatment. However, PGE2 accumulation was not affected by any of the antioxidants used. Expressions of COX-2 and iNOS were effectively reduced by 5 microM quercetin treatment. These findings indicate that dietary antioxidants possess significant anti-inflammatory activities, and quercetin is the most potent antioxidant.
Jung
Won-Jin
WJ
Department of Food and Nutrition, Sookmyung Women's University, 53-12 Chungpa-dong 2-ka, Yongsan-ku , Seoul, 140-742 Korea.
Sung
Mi-Kyung
MK
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2; D004032:Diet; D015232:Dinoprostone / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D011451:Prostaglandin-Endoperoxide Synthases / Q000187:drug effects / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology; D024505:Tocopherols / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism; D019207:beta Carotene / Q000494:pharmacology
D000975:Antioxidants; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D019207:beta Carotene; D011794:Quercetin; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D024505:Tocopherols