Serum albumin biosynthesis and secretion by resting and lectin stimulated human lymphocytes.
Normal human peripheral lymphocytes, cultured in serum-deprived medium, synthetized and released serum albumin and some glycoproteins into the culture supernatant. With the use of [3H]leucine, it was shown that this biosynthetic activity was increased about 2-3 times when the mitogenic lectin from Robinia pseudo acacia was added to the lymphocyte culture medium.
Goussault
Y
Y
Centre de Recherches sur les Protéines, Faculté de Médecine Lariboisière-Saint Louis, Universitê Paris VII, 45 Rue des Saints Pères, Paris, France.
Sharif
A
A
Bourrillon
R
R
D002478:Cells, Cultured; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D037121:Plant Lectins / Q000494:pharmacology*; D012709:Serum Albumin / Q000096:biosynthesis* / Q000378:metabolism*
D017077:Culture Media, Conditioned; D037121:Plant Lectins; C047720:Robinia lectin; D012709:Serum Albumin