title: Ultramorphology and histochemistry of fat body cells from last instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae).
abstract: The fat body cells of Pachycondyla (=Neoponera) villosa are disposed in a single layer between the cuticle and the digestive tract, forming a group of cells enclosed by a thin membrane. Histological studies have revealed three different cellular types: trophocyte (more abundant), urate, (located among the trophocytes), and oenocyte (the scarcest), usually observed laying near the cuticle. Histochemically, the trophocytes showed a positive reaction for basic proteins in the nucleus and cytoplasm, as well as a strong positive reaction in the cytoplasmic granules. The test for carbohydrates showed a strong positive reaction throughout the cytoplasm, while the test for lipids was positive for the cytoplasmic vesicles. The urate cells showed a positive reaction for basic proteins in the nucleus and in the areas of the cytoplasm surrounding the vesicles. These cells did not react to the PAS test or to Sudan Black B. The oenocytes showed a weak positive reaction to PAS and a strong positive reaction to Sudan Black B and Mercuric-bromophenol Blue.
authors.affiliation: UNESP, Campus Litoral Paulista, Unidade de São Vicente, Praça Infante Dom Henrique s/n Parque Bitaru, CEP 13330-900, São Vicente, SP, Brazil.
authors.forename: F J
authors.initials: FJ
authors.lastname: Zara
authors.forename: F H
authors.initials: FH
authors.lastname: Caetano
mesh_terms: D000818:Animals; D001000:Ants / Q000737:chemistry / Q000648:ultrastructure*; D002241:Carbohydrates / Q000032:analysis*; D005216:Fat Body / Q000737:chemistry / Q000648:ultrastructure*; D006651:Histocytochemistry; D019476:Insect Proteins / Q000032:analysis*; D007814:Larva / Q000737:chemistry / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D019476:Insect Proteins; D008055:Lipids