title: Promotion of U937 cell adhesion on polypropylene surfaces bearing phosphorylcholine functionalities.
abstract: Phosphorylcholine (PC) groups were grafted onto ammonia plasma-treated biaxially-oriented polypropylene (BOPP) surfaces, via (a) reductive amination of phosphorylcholine glyceraldehyde and (b) a two-step procedure involving the chemical amplification of surface amine groups with tris(2-aminoethyl amine) and subsequent reductive amination of phosphorylcholine glyceraldehyde. The occurrence of grafting was ascertained by X-ray photoelectron spectroscopy (XPS) and attenuated total reflectance Fourier-transform infrared (ATR-FT-IR) spectroscopy. The wettability of PC-modified surfaces was assessed by dynamic contact-angle measurements using the Wilhelmy plate method. Human U937 macrophages adhered and proliferated to a significantly larger extent on PC-modified surfaces, compared to unmodified or ammonia plasma-modified BOPP.
authors.affiliation: Department of Chemistry and Faculty of Pharmacy, University of Montreal, Montréal, Québec, Canada H3C 3J7.
authors.forename: Yong-Kuan
authors.initials: YK
authors.lastname: Gong
authors.forename: Fackson
authors.initials: F
authors.lastname: Mwale
authors.forename: Michael R
authors.initials: MR
authors.lastname: Wertheimer
authors.forename: Francoise M
authors.initials: FM
authors.lastname: Winnik
mesh_terms: D000641:Ammonia / Q000737:chemistry; D002448:Cell Adhesion; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D015394:Molecular Structure; D010767:Phosphorylcholine / Q000032:analysis* / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry* / Q000378:metabolism*; D013057:Spectrum Analysis; D020298:U937 Cells
chemical_list: D011126:Polypropylenes; D010767:Phosphorylcholine; D000641:Ammonia