[Obtaining of intertribal Brassica juncea+Arabidopsis thaliana somatic hybrids and study of transgenic trait behaviour].
Intertribal somatic hybridization between wild type Brassica juncea (L.) Czern. & Coss. and transgenic A. thaliana L. has been carried out. Genome of A. thaliana plants contained heterologous transposable element Spm/dSpm, reporter GUS gene, selective genes for kanamycin- (npt II) and phosphinothricin (bar) resistance. Hybrid nature of obtained plants was confirmed with their morphology, GUS hystochemical assay, PCR-RFLP, RAPD and isozyme analyses. It was determined that heterologous transposable element Spm/dSpm is able to function in hybrid plants. There was no complete elimination of A. thaliana genetic material in the hybrids and the transgenes were stably maintained.
Ovcharenko
O O
OO
Komarnyts'kyĭ
I K
IK
Cherep
M M
MM
Hleba
Iu Iu
IuIu
Kuchuk
M V
MV
D000681:Amylases / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004950:Esterases / Q000378:metabolism; D017343:Genes, Plant / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D006824:Hybridization, Genetic / Q000235:genetics*; D009149:Mustard Plant / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D018520:Plant Shoots / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000201:enzymology; D012641:Selection, Genetic
D017931:DNA Primers; D004251:DNA Transposable Elements; D018744:DNA, Plant; D004950:Esterases; D000681:Amylases