[Study on mutation of ATM/PI3K region in NPC cell lines with different radiosensitivity].
To try to elucidate the relationship between the radiosensitivity of NPC cell lines and ATM gene, this study was designed to investigate the mutation of ATM/PI3K region in NPC cell lines with different radiosensitivity. Two NPC cell lines of CNE1 and CNE2 with different radiosensitivities were established. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to get a 546bp fragment (8578nt-9123nt) of ATM cDNA, containing the PI-3 kinase domain (8753-8815bp). Direct sequencing of RT-PCR product was applied to determine the mutations in the gene. The sequence of the 546bp fragment is identical to the sequence of ATM gene published in GenBank, that is, there are no any mutations on the fragment of ATM/PI3K we examined either in CNE1 or in CNE2. It indicates that there may be no correlations between the mutation of ATM/PI3K and the variation of radiosensitivity in NPC cell lines CNE1, CNE2.
Wang
Hong-Mei
HM
Department of Forensic Medicine, Sun Yat-Sen University, Guangzhou 510080, China. hongmei0613@sina.com
Wu
Xin-Yao
XY
Xia
Yun-Fei
YF
Huang
Yan-Mei
YM
Liu
Xiu-Fang
XF