Development of an accelerated low-pH reversed-phase liquid chromatography column stability test.
The important experimental design criteria for an accelerated low-pH RPLC column stability test are discussed. The influence of method variables on the amount and rate of retention-loss and the final optimized parameters for the accelerated low-pH RPLC stability test are presented. The retention-loss curves for selected C8 and C18 stationary phases are compared. These studies indicate that ligand chain length, functionality and bonding density play an important role in determining the low-pH stability of a stationary phase. Additionally, elemental analysis data are used to infer the mechanism responsible for the observed retention-loss under low-pH conditions.
Trammell
Brian C
BC
Waters Corporation, 34 Maple Street, Milford, MA 01757, USA.
Boissel
Cheryl A
CA
Carignan
Christina
C
O'Shea
Daniel J
DJ
Hudalla
Christopher J
CJ
Neue
Uwe D
UD
Iraneta
Pamela C
PC
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D013696:Temperature