Heavy metal determination by biosensors based on enzyme immobilised by electropolymerisation.
The work describes the original application of biosensors based on enzyme immobilised by electropolymerisation to heavy metal determination. An inhibition detection scheme has been employed for detecting Hg2+ by an established glucose biosensor based on glucose oxidase immobilised in poly-o-phenylenediamine. The investigated enzymatic inhibition appears reversible and mixed, in agreement with data for the enzyme in solution. A low response time (<2 min) and a rapid recovery of response by EDTA seem the most interesting characteristics of the proposed biosensor at the present stage of development, along with the well known easy preparation of this kind of biosensors. The occurrence of a high response also for Cu2+ opens the possibility to apply the biosensor in total toxic metal content determination.
Malitesta
C
C
Laboratorio di Chimica Analitica, Dipartimento di Scienza dei Materiali, Università di Lecce, Via Arnesano, 73100 Lecce, Italy. cosimino.malitesta@unile.it
Guascito
M R
MR
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005949:Glucose Oxidase / Q000032:analysis / Q000037:antagonists & inhibitors*; D008628:Mercury / Q000032:analysis / Q000737:chemistry; D019216:Metals, Heavy / Q000032:analysis* / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry*; D011108:Polymers / Q000032:analysis / Q000737:chemistry; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D019216:Metals, Heavy; D010655:Phenylenediamines; D011108:Polymers; D005949:Glucose Oxidase; D008628:Mercury