Production of minichaperone (sht GroEL191-345) and its function in the refolding of recombinant human interferon gamma.
The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg/L broth. In the refolding of recombinant human interferon gamma (rhuIFN-gamma) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.
Guan
Yi-Xin
YX
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China.
Fei
Zheng-Zheng
ZZ
Luo
Man
M
Yao
Shan-Jing
SJ
Cho
Man-Gi
MG
D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry* / Q000378:metabolism; D017510:Protein Folding; D011994:Recombinant Proteins
D018834:Chaperonin 60; D011994:Recombinant Proteins; D007371:Interferon-gamma