Production of nitric oxide by hypoxic radiosensitizer sanazole.
OBJECTIVE
In this work we investigated the ability of hypoxia-selective radiosensitizer--sanazole to produce nitric oxide (NO).


METHODS
NO formation was determined by spectophotometric method in the reaction with sanazole and oxyhemoglobin. In suspensions of lymphoma EL-4 and mastocytoma P 8815 cell NO production was estimated indirectly as nitrite concentration in the supernatant fraction.


RESULTS
Transformation of oxyhemoglobin by sanazole to methemoglobin suggested the dissociation of nitro group in aqueous solution and denitration of molecules. Addition of sanazole to hypoxic tumor cell suspension resulted in the increase of nitrite content in tissue culture medium.


CONCLUSIONS
Presented data suggest the ability of sanazole to produce NO that may be important in a probable mechanism for antitumor and immunomodulating properties of this radiosensitizer.
Kondakova
Irina V
IV
Institute of Oncology, Russian Academy of Medical Sciences, Tomsk, Russia. oncology@info.tsu.ru
Tcheredova
Varvara V
VV
Zagrebelnaya
Gelena V
GV
Cherdyntseva
Nadezda V
NV
Kagiya
Tsutomu V
TV
Choinzonov
Evgeny L
EL
D000818:Animals; D015687:Cell Hypoxia; D008223:Lymphoma / Q000473:pathology*; D034801:Mastocytoma / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental; D009569:Nitric Oxide / Q000032:analysis*; D010108:Oxyhemoglobins / Q000378:metabolism; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*
D010108:Oxyhemoglobins; D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D009569:Nitric Oxide