[Demecolcine-induced enucleation of Kunming mouse oocyte].
On the basis of exiting technique pathway of demecolcine-induced enucleation(IE), several factors (Demecolcine concentration, time of demecolcine inition and treatment, oocytes age) affecting the IE rate were tested using Kunming mouse oocytes. The experiments' results demonstrated that: In experiment 1,activated oocytes could be enucleated efficiently by treating with KSOM medium containing 0.4 microg/mL or 0.5 microg/mL demecolcine for 60 min, but 0.5 microg/mL group gained the higher IE rate(33.3%). In experiment 2, maximum IE rate (31.9% -approximately 24.5%) were obtained when oocytes were exposed to 0.5 microg/mL demecolcine between 0 and 5 min postactivation and treated for 60 -approximately 180 min. In experiment 3,oocytes collected from Kunming mouse at 17 -approximately 18h after hCG administration were favoriate to demecolcine-IE(27.1%). By comparison and analysis of the data, we established the optimized IE procedure.
Wang
Qiang
Q
Institute of Bio-Engineering, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi, Yangling 712 100,China.
An
Zhi-Xing
ZX
Gu
Ling
L
Ma
Li-Bing
LB
Zheng
Yue-Mao
YM
Zhang
Yong
Y
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects*; D019976:Cloning, Organism / Q000379:methods*; D003703:Demecolcine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D009865:Oocytes / Q000187:drug effects*; D013997:Time Factors
D003703:Demecolcine