[The development and collagen ingredients analysis of submandibular gland acellular matrix].
OBJECTIVE
To develop submandibular gland acellular matrix and observe its structure and analyze its histological ingredients.


METHODS
The fresh submandibular glands of SD mice were cell-extracted by chemical detergent and histological ingredients were analyzed by immunohistochemistry.


RESULTS
Submandibular gland cells disappeared, matrix ingredients appeared network structure under microscopy. I, II and III type collagen proteins of submandibular gland before and after cell-extracted were positive under immunohistochemistry.


CONCLUSIONS
The main ingredients of submandibular gland acellular matrix were I, II and III type collagen proteins.
Deng
Chun-fu
CF
Department of Oral and Maxillofacial Surgery, First Clinical Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
Tan
Xue-xin
XX
Zhou
Qing
Q
Huang
Shao-hui
SH
Xu
Zhong-fei
ZF
Wang
Yu-xin
YX
D062088:Acellular Dermis / Q000378:metabolism*; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D013363:Submandibular Gland / Q000378:metabolism*
D003094:Collagen