[The I1307K mutation and protein expression of APC gene in gastric cancer].
In order to explore the correlation of the abnormalities of tumor suppressor gene APC with the carcinogenesis and progression of gastric cancer. The I1307K mutation of APC gene in gastric cancer was analysed using Amplification Refractory Mutation System PCR(ARMS ,PCR),also the expression of APC protein in gastric cancer of different stages was detected by immunohistochemical method. We found that there wasn't I1307K mutation of APC gene in 62 cases of blood samples of susceptible population in high incidence areas of gastric cancer and 45 cases of gastric cancer tissues. The positive rates of APC protein in gastric cancer (both early and progressive gastric cancer) were significantly lower than that in normal mucosa,the positive rates of APC protein in progressive gastric cancer were significantly lower than that in early gastric cancer,the positive rates of APC protein in gastric cancer with lymph node metastasis were significantly lower than that in gastric cancer without lymph node metastasis. So it was thought that there might be no correlation between the I1307K mutation of APC gene and carcinogenesis of gastric cancer in China,but the decreased expression of APC protein was closely related to the carcinogenesis,progression and lymph node metastasis of gastric cancer.
Sun
Xiu-Ju
XJ
Department of Molecular Genetics, China Medical University, Shenyang 110001, China. sun1019@hotmail.com
Zheng
Zhi-Hong
ZH
Fu
Hao
H
Xu
Hui-Mian
HM
Hao
Dong-Mei
DM
Yuan
Yuan
Y
Sun
Kai-Lai
KL