The effect of adult hemoglobin on red blood cell nitric oxide levels during fetal development.
OBJECTIVE
To compare the levels of S-nitrosohemoglobin (HbSNO) at different gestational ages in newborn infants and correlate the levels of HbSNO with HbA and HbF.


METHODS
Cord blood samples of 22 newborn infants of different gestational ages (25-41 weeks) were analyzed. The levels of HbF and HbA were determined by HPLC and of HbSNO by chemiluminescence.


RESULTS
The level of HbSNO varied from 28.1 to 145.3 nM. There was a significant correlation with gestational age (r(2) = 0.5469, p < 0.0001) and with the relative amount of HbA (r(2) = 0.8144, p < 0.0001).


CONCLUSIONS
The increases in HbSNO in fetal red cells is directly related to the relative amount of HbA.
Bard
Harry
H
Pediatric Department, Sainte-Justine's Hospital, University of Montreal, Montreal, Canada. harry.bard@umontreal.ca
English
Ann M
AM
Gagnon
Carmen
C
Bellemin
Karine
K
D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000378:metabolism*; D005312:Fetal Blood; D047109:Fetal Development; D005319:Fetal Hemoglobin / Q000378:metabolism*; D006441:Hemoglobin A / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D008163:Luminescent Measurements
D006454:Hemoglobins; C103574:S-nitrosohemoglobin; D006441:Hemoglobin A; D005319:Fetal Hemoglobin