title: On peptide de novo sequencing: a new approach.
abstract: A procedure is presented for the automatic determination of the amino acid sequence of peptides by processing data obtained from mass spectrometry analysis. This is a basic and relevant problem in the field of proteomics. Furthermore, it has an even higher conceptual and applicative interest in peptide research, as well as in other connected fields. The analysis does not rely on known protein databases, but on the computation of all amino acid sequences compatible with the given spectral data. By formulating a mathematical model for such combinatorial problems, the structural limitations of known methods are overcome, and efficient solution algorithms can be developed. The results are very encouraging both from the accuracy and computational points of view.
authors.affiliation: PolyDART: Data Analysis Research Team for Polymers, 03015 Fiuggi (FR), Italy. bruni@dis.uniroma1.it
authors.forename: Renato
authors.initials: R
authors.lastname: Bruni
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Gianfranceschi
authors.forename: Giorgio
authors.initials: G
authors.lastname: Koch
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D013058:Mass Spectrometry; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D020539:Sequence Analysis, Protein
chemical_list: D010455:Peptides