title: Cytoplasmic-to-nuclear volume ratio affects AP-1 complex formation as an indicator of cell cycle responsiveness.
abstract: Cytoplasmic volume undergoes a series of changes during mitosis in eukaryotes; in turn, signaling events such as osmotic stress can regulate the cytoplasmic volume in cells. In some organisms, increase in cytoplasmic-to-nuclear volume ratio was seen to affect the growth potential in cells, however, the mechanistics of such a regulation, if at all present, was unclear. In a computational model, we have constructed a growth factor-induced signaling pathway leading to AP-1 heterodimer formation through transcriptional regulation, and analyzed the effects of increasing the cytoplasmic-to-nuclear ratio on c-jun transcription and AP-1 complex. We have observed that larger cytoplasmic volumes caused both an increase in the final AP-1 product and a delay in the time of AP-1 accumulation.
authors.affiliation: Institute of Biomedical Engineering, Boğaziçi University, Bebek, Istanbul, Turkey.
authors.forename: Izzet
authors.initials: I
authors.lastname: Oney
authors.forename: Isil Aksan
authors.initials: IA
authors.lastname: Kurnaz
authors.forename: M Levent
authors.initials: ML
authors.lastname: Kurnaz
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000502:physiology*; D048429:Cell Size; D019295:Computational Biology; D003198:Computer Simulation; D003593:Cytoplasm / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000494:pharmacology / Q000502:physiology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000502:physiology; D008954:Models, Biological; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000502:physiology; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D018808:Transcription Factor AP-1 / Q000378:metabolism*
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D004815:Epidermal Growth Factor; D020929:Mitogen-Activated Protein Kinase Kinases