In vivo and in vitro tissue-specific expression of green fluorescent protein using the cre-lox system in mouse embryonic stem cells.
Embryonic stem cells (ES) are pluripotent and may therefore serve as a source for the generation of specific cell types required for future therapies based on cell replacement. The isolation of defined cell populations from a certain lineage or tissue is a prerequisite for the analysis of the potential of such ES-derived cells in animal transplantation studies. Here, using the Cre/loxP system, we report the generation of murine ES cells conditionally expressing the hrGFP gene at the cell surface. Such ES cells can be differentiated in vitro into neurons displaying GFP activity in neurites. Transgenic mice derived from these ES cells permit the targeting of GFP-expression to specific tissues and provide material from the three germ layers suitable for molecular and biochemical analysis.
Schindehütte
Jan
J
Georg-August University of Göttingen, Department of Clinical Neurophysiology, Göttingen, Germany.
Fukumitsu
Hidefumi
H
Collombat
Patrick
P
Griesel
Gundula
G
Brink
Christopher
C
Baier
Paul C
PC
Capecchi
Mario R
MR
Mansouri
Ahmed
A
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases