[HLA-DR-positive T lymphocytes in the bone marrow and peripheral blood of patients with severe aplastic anemia and their in vitro inhibitory effecton BFU-E and CFU-GM].
OBJECTIVE
[corrected] To elucidate the role of immunodysfunction in the pathogenesis of severe aplastic anemia (SAA).


METHODS
HLA-DR+ T cells were detected in bone marrow (BM) and peripheral blood (PB) of 20 SAA patients and controls (9 for BM and 11 for PB). PHA-LYCM conditioned medium was prepared, and its in vitro effect on normal bone marrow BFU-E and CFU-GM was studied.


RESULTS
The percentages of HLA-DR+ T lymphocytes in BM and PB of newly diagnosed SAA patients were significantly higher than that of controls (P<0.001), and that of recovered SAA (rSAA) patients after ALG therapy (P<0.05). Compared with controls, the PBA-LYCMs from newly diagnosed SAA patients showed significant inhibitory effect on normal BFU-E and CFU-GM (P<0.001).


CONCLUSIONS
The activated T lymphocyte may play an important role in the pathogenesis of SAA.
Xia
C
C
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
Chu
Y
Y
Zhang
J
J
Shao
Z
Z
Tian
Z
Z
Chen
G
G
Tang
X
X
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000188:drug therapy / Q000276:immunology* / Q000628:therapy; D000961:Antilymphocyte Serum / Q000627:therapeutic use; D016022:Case-Control Studies; D002648:Child; D015672:Erythroid Precursor Cells / Q000276:immunology*; D055014:Granulocyte-Macrophage Progenitor Cells / Q000276:immunology*; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D016896:Treatment Outcome; D055815:Young Adult
D000961:Antilymphocyte Serum; D006684:HLA-DR Antigens