Biosynthesis of iso-fatty acids in myxobacteria: iso-even fatty acids are derived by alpha-oxidation from iso-odd fatty acids.
The biosynthesis of iso-fatty acids was investigated in the myxobacteria Myxococcus xanthus and Stigmatella aurantiaca by feeding labeled precursors of these fatty acids and by conducting detailed analysis of the fatty acid profiles and fatty acid MS and NMR spectra. These experiments revealed that in S. aurantiaca all iso-even fatty acids are formed via alpha-oxidation of iso-odd fatty acids and not by using valine-derived isobutyryl-CoA.
Bode
Helge B
HB
Institut für Pharmazeutische Biotechnologie, Universität des Saarlandes, P.O. Box 151150, 66041 Saarbrücken, Germany.
Dickschat
Jeroen S
JS
Kroppenstedt
Reiner M
RM
Schulz
Stefan
S
Müller
Rolf
R
D000596:Amino Acids / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D013058:Mass Spectrometry; D009231:Myxococcales / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D013302:Streptomyces / Q000378:metabolism
D000596:Amino Acids; D005227:Fatty Acids