Three-dimensional type I collagen cell culture systems for the study of bone pathophysiology.
Three-dimensional (3-D) type I collagen cell culture systems composed of reconstituted collagen fibres are able to support short- and long-term growth of various cell types, including cancer cell lines, endothelial cells, endometrial cells, hepatocytes, osteoblasts and fibroblasts and to sustain or even enhance cell differentiation, in vitro. In addition, 3-D culture systems have been successfully used in the investigation of complex biological processes, such as angiogenesis, wound healing, tumour invasion and metastasis. The latter suggested that 3-D culture systems have the potential to simulate cell-cell interactions, which take place in tissues under physiological and pathophysiological conditions. This review focuses on the investigational use of 3-D collagen cell culture systems in bone physiology and the pathophysiology of skeletal metastasis.
Themistocleous
G S
GS
Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece.
Katopodis
H
H
Sourla
A
A
Lembessis
P
P
Doillon
C J
CJ
Soucacos
P N
PN
Koutsilieris
M
M
D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism / Q000503:physiopathology* / Q000556:secondary; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D002450:Cell Communication; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000166:cytology*; D024042:Collagen Type I / Q000378:metabolism; D006801:Humans
D024042:Collagen Type I