title: Effects of elastase on the mechanical and failure properties of engineered elastin-rich matrices.
abstract: Pulmonary emphysema and vessel wall aneurysms are diseases characterized by elastolytic damage to elastin fibers that leads to mechanical failure. To model this, neonatal rat aortic smooth muscle cells were cultured, accumulating an extracellular matrix rich in elastin, and mechanical measurements were made before and during enzymatic digestion of elastin. Specifically, the cells in the cultures were killed with sodium azide, the cultures were lifted from the flask, cut into small strips, and fixed to a computer-controlled lever arm and a force transducer. The strips were subjected to a broadband displacement signal to study the dynamic mechanical properties of the samples. Also, quasi-static stress-strain curves were measured. The dynamic data were fit to a linear viscoelastic model to estimate the tissues' loss (G) and storage (H) modulus coefficients, which were evaluated before and during 30 min of elastase treatment, at which point a failure test was performed. G and H decreased significantly to 30% of their baseline values after 30 min. The failure stress of control samples was approximately 15 times higher than that of the digested samples. Understanding the structure-function relationship of elastin networks and the effects of elastolytic injury on their mechanical properties can lead to the elucidation of the mechanism of elastin fiber failure and evaluation of possible treatments to enhance repair in diseases involving elastolytic injury.
authors.affiliation: Dept. of Biochemistry, 715 Albany St., Boston, MA 02118, USA.
authors.forename: Lauren D
authors.initials: LD
authors.lastname: Black
authors.forename: Kelly K
authors.initials: KK
authors.lastname: Brewer
authors.forename: Shirley M
authors.initials: SM
authors.lastname: Morris
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Schreiber
authors.forename: Paul
authors.initials: P
authors.lastname: Toselli
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Nugent
authors.forename: Bela
authors.initials: B
authors.lastname: Suki
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Stone
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D004548:Elasticity; D004549:Elastin / Q000737:chemistry* / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000502:physiology*; D010196:Pancreatic Elastase / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013718:Tensile Strength
chemical_list: D002457:Cell Extracts; D004549:Elastin; D010196:Pancreatic Elastase