Solution state conformation and degradation of cyclopeptides containing an NGR motif.
In contrast to the RGD-peptides, head to tail cyclization of LNGRV and LNGRv caused only a marginal change in their integrin receptor affinity as shown by the limited effect and selectivity on the adhesion of endothelial cells to ECM components. Structure determination of the two cyclopeptides by NMR and MD, semiempirical and ab initio methods revealed that both are very flexible and take on multiple stable conformers in solution. This structural diversity, along with the presence of the Asn-Gly peptide bond, enhances succinimide ring formation leading to the hydrolysis of Asn. It has been demonstrated that c(LNGRV) suffers deamidation with time both in solution and during storage. As the isoaspartyl-peptide may co-elute with the asparginyl-peptide in the course of HPLC analysis, MS measurement is necessary to check the purity of peptides containing the NGR sequence. Our stability investigations raise the question whether the NGR motif or its hydrolysis product is effective in in vivo experiments.
Füzéry
Anna K
AK
Department of Organic Chemistry, Eötvös Loránd University, Budapest, Hungary.
Mihala
Nikolett
N
Szabó
Pál
P
Perczel
András
A
Giavazzi
Raffaella
R
Süli-Vargha
Helga
H
D020816:Amino Acid Motifs; D001120:Arginine / Q000737:chemistry; D001216:Asparagine / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004730:Endothelium, Vascular / Q000166:cytology; D005998:Glycine / Q000737:chemistry; D006801:Humans; D016023:Integrins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization
D016023:Integrins; D010456:Peptides, Cyclic; D001216:Asparagine; D001120:Arginine; D005998:Glycine