Regulation of tau exon 10 splicing by a double stem-loop structure in mouse intron 10.
Tau exon 10 (E10) splicing is a crucial event in its developmental change of tau isoform and tauopathy. To investigate the splicing mechanism, we isolated and compared mouse tau genomic sequence with human sequence. We identified a new element in mouse intron 10 (I10) to suppress E10 splicing, which was located just after the stem-loop region previously proposed in human sequence and found to potentially form another stem-loop. Human I10 with a mutation (+29G to A) causing a decreased E10 splicing was also predicted to form similar double stem-loop, suggesting that this element is universally involved in regulation of E10 splicing.
Yamashita
Takenari
T
Department of Neuroscience, Osaka City University Medical School, 1-4-3 Asahimachi, Abenoku, Osaka 545-8585, Japan.
Tomiyama
Takami
T
Li
Qiang
Q
Numata
Hideharu
H
Mori
Hiroshi
H
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005091:Exons / Q000235:genetics*; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D007438:Introns / Q000235:genetics*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D024801:Tauopathies / Q000235:genetics; D016875:tau Proteins / Q000235:genetics*
C054369:MAPT protein, human; D008869:Microtubule-Associated Proteins; D016875:tau Proteins