[The relationship between beta fibrinogen gene-455G/A polymorphisms and Budd-Chiari syndrome].
OBJECTIVE
To study the effect of the -455G-->A substitution on influencing fibrinogen levels and morbidity in patients with Budd-Chiari syndrome (BCS).


METHODS
53 patients with BCS diagnosed by color Doppler-ultrasound and venography and 105 healthy persons as control were observed. Assay of plasma fibrinogen was performed by the method of enzymatic reaction. DNA was extracted from white cells using the phenol/chloroform method. beta fibrinogen gene was detected by polymerase chain reaction- restriction fragment length polymorphism techniques using thermostable Taq polymerase under conditions recommended by the manufacturer.


RESULTS
The frequencies of -455GA+AA genotype were significantly increased in patients with BCS compared with normal controls (P <0.05, OR=2.04, 95% CI: 1.03-4.02). There was a significant difference in plasma fibrinogen levels between BCS subjects and control subjects (P <0.01). Either in patients with BCS or in healthy controls, the plasma fibrinogen levels was significantly increased seen in the subjects with -455A alleles (0.01< P <0.05).


CONCLUSIONS
beta fibrinogen gene -455G/A polymorphism is associated with increased plasma fibrinogen levels and may be an important risk factor in the pathogenesis of BCS.
Wang
Su-yun
SY
Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, China.
Wei
Ying-fei
YF
Li
Jing-dong
JD
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D006502:Budd-Chiari Syndrome / Q000235:genetics*; D005260:Female; D005340:Fibrinogen / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
C115499:BBeta fibrinogen; D005340:Fibrinogen