title: Isoprostanes, prostaglandins and tocopherols in pre-eclampsia, normal pregnancy and non-pregnancy.
abstract: This study is designed to evaluate whether oxidative stress and inflammation are involved in severe pre-eclampsia compared to normal pregnancy and non-pregnancy. We have measured plasma and urinary levels of 8-iso-PGF2alpha, a major isoprostane as an indicator of oxidative stress; plasma and urinary 15-keto-dihydro-PGF2alpha, a major metabolite of cyclooxygenase-catalysed PGF2alpha as an indicator of inflammatory response, and plasma -alpha-and -gamma-tocopherol in 18 pre-eclamptic, 19 normal pregnancy and 20 non-pregnant women. Pregnant women had significantly higher levels of 8-iso-PGF2alpha and PGF2alpha metabolite as compared to the non-pregnancy. Levels of 8-iso-PGF2alpha in the pre-eclamptic women did not differ from the normal pregnancy but PGF2alpha metabolite levels were significantly higher in normal pregnancy. On the other hand, gamma-tocopherol levels were significantly lower in pre-eclampsia than normal pregnancy. In contrast, the concentration of alpha-tocopherol was very similar between the groups. alpha-and gamma-tocopherol levels were significantly lower in pregnancy compared to non-pregnancy. Although no direct evidence of oxidative stress and inflammatory response was observed in severe pre-eclampsia, a reduction of gamma-tocopherol suggests the possible precedence of oxidative stress in this condition. Higher levels of isoprostanes and prostaglandin metabolite in late pregnancy suggest the importance of both free radicals and cyclooxygenase-catalysed oxidation products in normal biological processes of pregnancy.
authors.affiliation: Department of Obstetrics and Gynaecology, Saitama Medical School, Moroyama, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ishihara
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeda
authors.forename: Bengt
authors.initials: B
authors.lastname: Vessby
authors.forename: Samar
authors.initials: S
authors.lastname: Basu
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000652:urine; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D011225:Pre-Eclampsia / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011247:Pregnancy; D011263:Pregnancy Trimester, Third / Q000097:blood / Q000652:urine; D013548:Sweden; D024502:alpha-Tocopherol / Q000097:blood; D024504:gamma-Tocopherol / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C075750:8-epi-prostaglandin F2alpha; D024504:gamma-Tocopherol; D015237:Dinoprost; D024502:alpha-Tocopherol