[A new blocking method for DNA chip].
Suggested a new blocking method for DNA microarray: 1-iodo-propane. We compared the difference between the two block reagents and the blocking results in different blocking time. The results showed that 1-I-propane is batter-blocking reagent than succinic anhydride in DNA chip. 1h blocking time is enough for blocking.
He
Qun
Q
Center of Biochip, China Medical University, Shenyang 110 001, China. hhqqbear@sina.com.ccn
Zhao
Yu-Jie
YJ
D006847:Hydrocarbons, Iodinated; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013387:Succinic Anhydrides / Q000737:chemistry
D006847:Hydrocarbons, Iodinated; D013387:Succinic Anhydrides; C031801:succinic anhydride; C509670:propyl iodide