title: The energy profiling of short interfering RNAs is highly predictive of their activity.
abstract: The technology of knockdown of gene expression by synthetic short interfering RNAs (siRNAs) is currently diffused because of its relative simplicity and reliability. Despite the claims that active siRNAs can be easily identified, how to select active siRNAs with high efficiency it is not fully defined. Here, we report that energy profiling can be used in the guidelines for siRNA design.
authors.affiliation: Laboratorio di Terapia Genica e Molecolare, Istituto di Fisiologia Clinica, CNR, Pisa, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Poliseno
authors.forename: Monica
authors.initials: M
authors.lastname: Evangelista
authors.forename: Alberto
authors.initials: A
authors.lastname: Mercatanti
authors.forename: Laura
authors.initials: L
authors.lastname: Mariani
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Citti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Rainaldi
mesh_terms: D000818:Animals; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics; D013552:Swine; D013816:Thermodynamics
chemical_list: D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins