A study of signal transduction for the two diuretic peptides of Diploptera punctata.
We investigated second messengers involved in the action of the CRF-related peptide Dippu-DH46 and the calcitonin-like peptide Dippu-DH31 in Diploptera punctata. Dippu-DH46 causes a dose-dependent increase in intracellular cAMP levels, its diuretic activity is mimicked by cAMP agonists, but is attenuated by Rp-cAMPS. Dippu-DH46 acts synergistically with kinins and thapsigargin; both mobilize intracellular Ca2+. Dippu-DH46 also acts synergistically with cAMP agonists, and its effect is inhibited by a PKC inhibitor, suggesting it also activates intracellular Ca2+. Dippu-DH31 has no effect on cAMP levels and its activity is not blocked by cAMP agonists. Neither peptide stimulated cGMP levels in a dose-dependent manner, nor does cGMP have any effect on fluid secretion.
Tobe
S S
SS
Department of Zoology, University of Toronto, Toronto, Canada ON M5S 3G5.
Zhang
J R
JR
Schooley
D A
DA
Coast
G M
GM
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D003058:Cockroaches / Q000378:metabolism*; D004232:Diuretics / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology; D007705:Kinins / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000096:biosynthesis / Q000494:pharmacology; D010455:Peptides / Q000187:drug effects / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D019284:Thapsigargin / Q000494:pharmacology; D013997:Time Factors
D004232:Diuretics; D007211:Indoles; D007705:Kinins; D008301:Maleimides; D009712:Nucleotides, Cyclic; D010455:Peptides; D019284:Thapsigargin; D011493:Protein Kinase C; D000252:Calcium-Transporting ATPases; C070515:bisindolylmaleimide I