Benzyl isothiocyanate inhibits oxidative stress in mouse skin: Involvement of attenuation of leukocyte infiltration.
The exposure of benzyl isothiocyanate (BITC) to mouse skin resulted in the attenuation of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative damage through not only inhibition of the NADPH oxidase system but also leukocyte clearance at inflamed region. In spite of little ability to affect TPA-induced edema formation, pretreatments of mouse skin with BITC before the first or second TPA treatment significantly decrease the H2O2 level. A histological study also demonstrated that BITC enhanced the terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL)-positive index in mouse skin, suggesting that BITC might accelerate the disappearance of infiltrated leukocytes. Thus, these gathered data further supported that BITC has a potential as an anti-inflammatory agent.
Nakamura
Yoshimasa
Y
Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan. yossan@agr.nagoya-u.ac.jp
Miyoshi
Noriyuki
N
Takabayashi
Satoko
S
Osawa
Toshihiko
T
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007249:Inflammation / Q000517:prevention & control*; D017879:Isothiocyanates / Q000494:pharmacology*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D012867:Skin / Q000187:drug effects / Q000473:pathology*; D012879:Skin Physiological Phenomena; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; C031403:benzyl isothiocyanate; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate