title: Establishment of stable hFis1 knockdown cells with an siRNA expression vector.
abstract: Yeast Fis1p participates in mitochondrial fission, together with Dnm1p and Mdv1p. Recently, human Fis1 (hFis1) was reported to be involved in mitochondrial fission, together with Drp1. We established stable transformants with an hFis1 siRNA expression vector. In the stable hFis1 knockdown cells, hFis1 expression was suppressed to approximately 10%, and mitochondrial fission, induced by cisplatin treatment, was delayed. In addition, mouse Fis1 (mFis1) expression promoted mitochondrial fission and cell death in the hFis1 knockdown cells, suggesting that mFis1 complements the function of hFis1. These hFis1 siRNA expression vectors may be useful for studying the molecular function of mammalian Fis1.
authors.affiliation: Protein Research Group and Genome Exploration Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Arai
authors.forename: Kaori
authors.initials: K
authors.lastname: Ito
authors.forename: Motoaki
authors.initials: M
authors.lastname: Wakiyama
authors.forename: Eiko
authors.initials: E
authors.lastname: Matsumoto
authors.forename: Ayako
authors.initials: A
authors.lastname: Sakamoto
authors.forename: Yuuka
authors.initials: Y
authors.lastname: Etou
authors.forename: Makiko
authors.initials: M
authors.lastname: Otsuki
authors.forename: Mio
authors.initials: M
authors.lastname: Inoue
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyagishi
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002945:Cisplatin / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D005816:Genetic Complementation Test; D005821:Genetic Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; C476525:FIS1 protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D034741:RNA, Small Interfering; D002945:Cisplatin