Kinetics of glutamate and gamma-aminobutyric acid in cerebrospinal fluid in a canine model of complex partial status epilepticus induced by kainic acid.
An imbalance of excitatory and inhibitory transmitters in the brain has been suggested to cause epileptic seizures. In this study, we investigated the kinetics of glutamate (GLU) and gamma-aminobutyric acid (GABA) in cerebrospinal fluid (CSF-GLU and CSF-GABA, respectively) using a high performance liquid chromatography (HPLC) in a canine model of complex partial status epilepticus (CPSE) induced by the microinjection of kainic acid (KA) into the unilateral amygdala. During the acute phase (3, 6, 12 and 48 hr after the onset of CPSE), CSF-GLU was significantly increased, while CSF-GABA was decreased, although not significantly. In the chronic phase, both CSF-GLU and CSF-GABA were significantly lower than normal at 72 hr after the onset of CPSE, and their levels returned to normal at 2 months. Results of the present study demonstrate that CSF-GLU is gradually increased in relation with seizure severity, and suggested the possibility that CSF-GABA was consistently decreased during CPSE induced by KA in dogs.
Hasegawa
Daisuke
D
Department of Veterinary Radiology, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
Matsuki
Naoaki
N
Fujita
Michio
M
Ono
Kenichiro
K
Orima
Hiromitsu
H
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004285:Dogs; D018698:Glutamic Acid / Q000134:cerebrospinal fluid*; D007608:Kainic Acid / Q000633:toxicity*; D007700:Kinetics; D008845:Microinjections; D013226:Status Epilepticus / Q000139:chemically induced*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000134:cerebrospinal fluid*
D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D007608:Kainic Acid