[In vitro induction and expansion of dendritic cells from rat bone marrow and their characterization].
OBJECTIVE
To induce and expand dendritic cells (DC) from rat bone marrow in vitro and identify their biological characterization.


METHODS
The rat bone marrow cells were collected and cultured for 48 hours and the floating cells were removed. Then IL-4 and GM-CSF were added into the fresh medium. After 2 weeks, the morphological character of the cultured DCs was observed under light microscope and transmission electron microscope. Expressions of MHC class II molecule, B7-1 and B7-2 were detected by flow cytometry. The cultured DCs were co-cultured with allogenic T cells derived from rat spleen. T cell proliferation was measured by MTT colorimetry.


RESULTS
The cultured DCs had the typical morphological characterization of DC, and the expression rates of MHC class II molecule, B7-1 and B7-2 were 74.2%, 81% and 76% respectively. The cultured DCs could notably stimulate the proliferation of allogeneic T cells.


CONCLUSIONS
The adherent culture of rat bone marrow cells, and co-culture with IL-4 and GM-CSF can obtain a number of high purity of DCs, which lay the foundation for study on DC's function.
Wang
Yun-Fu
YF
Department of Neurology, Taihe Hospital of Yunyang Medical College, Shiyan 442000, China. wyfymc@sina.com
Sun
Sheng-Gang
SG
He
Guo-Hou
GH
Chen
Ji-Xiang
JX
Yang
Jing-Ning
JN
Li
Luo-Qing
LQ
Cao
Xue-Bing
XB
Wu
Xiong-Wen
XW
D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D049109:Cell Proliferation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology
D000949:Histocompatibility Antigens Class II