title: Salinity inhibits post transcriptional processing of chloroplast 16S rRNA in shoot cultures of jojoba (Simmondsia chinesis).
abstract: Chloroplast metabolism is rapidly affected by salt stress. Photosynthesis is one of the first processes known to be affected by salinity. Here, we report that salinity inhibits chloroplast post-transcriptional RNA processing. A differentially expressed 680-bp cDNA, containing the 3' sequence of 16S rRNA, transcribed intergenic spacer, exon 1 and intron of tRNA(Ile), was isolated by differential display reverse transcriptase PCR from salt-grown jojoba (Simmondsia chinesis) shoot cultures. Northern blot analysis indicated that although most rRNA appears to be fully processed, partially processed chloroplast 16S rRNA accumulates in salt-grown cultures. Thus, salinity appears to decrease the processing of the rrn transcript. The possible effect of this decreased processing on physiological processes is, as yet, unknown.
authors.affiliation: Department of Life Sciences and Doris and Bertie Center for Bioenergetics in Life Sciences, Ben-Gurion University, Beer-Sheva, 84105, Israel.
authors.forename: Ela
authors.initials: E
authors.lastname: Mizrahi-Aviv
authors.forename: David
authors.initials: D
authors.lastname: Mills
authors.forename: Aliza
authors.initials: A
authors.lastname: Benzioni
authors.forename: Dudy
authors.initials: D
authors.lastname: Bar-Zvi
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant; D019684:Magnoliopsida / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018520:Plant Shoots / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D018749:RNA, Plant / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*; D012492:Salts; D046509:Tissue Culture Techniques
chemical_list: D018744:DNA, Plant; D018749:RNA, Plant; D012336:RNA, Ribosomal, 16S; D012492:Salts