Phospholipase D-catalyzed synthesis of novel phospholipid-phytosterol conjugates.
In the present study, plant sterols were modified to form amphiphiles by synthesizing phospholipid derivatives from them so they could be formulated in different functional foods and possibly improve their effects as therapeutic agents. The new phosphatidyl derivatives, phosphatidyl-sitosterols, were synthesized by the transfer reaction of a phosphatidyl residue from PC to beta-sitosterol by phospholipase D (PLD EC 3.1.4.4) from Streptomyces sp. in a biphasic medium.
Hossen
Monjur
M
Food Protein R&D Center, Texas A&M University, College Station, Texas 77843-2476, USA.
Hernandez
Ernesto
E
D002384:Catalysis; D002855:Chromatography, Thin Layer; D015394:Molecular Structure; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D010840:Phytosterols / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013302:Streptomyces / Q000201:enzymology
D010743:Phospholipids; D010840:Phytosterols; D010739:Phospholipase D