The crystal structure of human adenylate kinase 6: An adenylate kinase localized to the cell nucleus.
Adenylate kinases (AKs) play important roles in nucleotide metabolism in all organisms and in cellular energetics by means of phosphotransfer networks in eukaryotes. The crystal structure of a human AK named AK6 was determined by in-house sulfur single-wavelength anomalous dispersion phasing methods and refined to 2.0-A resolution with a free R factor of 21.8%. Sequence analyses revealed that human AK6 belongs to a distinct subfamily of AKs present in all eukaryotic organisms sequenced so far. Enzymatic assays show that human AK6 has properties similar with other AKs, particularly with AK5. Fluorescence microscopy showed that human AK6 is localized predominantly to the nucleus of HeLa cells. The identification of a nuclear-localized AK sheds light on nucleotide metabolism in the nucleus and the energetic communication between mitochondria and nucleus by means of phosphotransfer networks.
Ren
Hui
H
National Laboratory of Protein Engineering and Plant Genetic Engineering and Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China.
Wang
Liya
L
Bennett
Matthew
M
Liang
Yuhe
Y
Zheng
Xiaofeng
X
Lu
Fei
F
Li
Lanfen
L
Nan
Jie
J
Luo
Ming
M
Eriksson
Staffan
S
Zhang
Chuanmao
C
Su
Xiao-Dong
XD
D021581:Active Transport, Cell Nucleus; D000263:Adenylate Kinase / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism
D007527:Isoenzymes; D009711:Nucleotides; D000263:Adenylate Kinase; C118782:adenylate kinase 5