[Integration sites of transgenes in transgenic animals].
To study the mechanism of transgene's integration in the host genome, first step is to clone the integration-site sequence. The method employed for this purpose includes selection from genomic pool, IPCR, TAIL-PCR and plasmid rescued. Different method fits different cases depending on how the transgenics have been produced. The data of integration-site sequences showed some similar features existed.
Wu
Bo
B
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Science, Wuhan 430072, China. wuboy530@sohu.com
Zhu
Zuo-Yan
ZY