[Detection of differentially expressed genes in hepatocellularcarcinoma cells SMMC-7721 treated with Typhonium giganteum extract by mRNA differential display].
OBJECTIVE
To screen and identify the differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responsing to the aqueous extract from dried powdered rhizomes of Typhonium giganteum (AEoTGE).


METHODS
The response of hepatocellular carcinoma cells SMMC-7721 to AEoTGE was explored with the technique of mRNA differential display.


RESULTS
After hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours, 1 gene expression was upgrade and 1 gene expression was downgrade induced by AEoTGE.


CONCLUSIONS
The research has provided important clues for the molecular mechanism of how hepatocarcinoma cells responseing to T. giganteum.
Wang
Shun-qi
SQ
Molecular Cytogenetics Lab, Nankai University, Tianjin 300071, China.
Ni
Hong
H
Cheng
Hua
H
Wang
Guang-liang
GL
Wang
Tong-shun
TS
Chen
Li
L
D029064:Araceae / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D012334:RNA, Neoplasm / Q000235:genetics; D027343:Rhizome / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D012334:RNA, Neoplasm