The stretched intermediate model of B-Z DNA transition.
There have been numerous attempts to describe the mechanism of B-Z transition. Our simulations based on the stochastic difference equation with length algorithm show that a short DNA oligomer will tend to unwind and overstretch during the transition. The overstretching of DNA is then understood from the Zhou, Zhang, and Ou-Yang model. Unlike the Harvey model, the stretched intermediate model does not pose any steric dilemma; we are able to show that the chain sense reversal progresses spontaneously using the stretched intermediate model. A nonlinear DNA model is used to describe the origins and mechanism of base rotation in the stretched intermediate state of DNA. We also propose an experiment that can verify the existence of a stretched intermediate state during B-Z transition, thus opening up fresh grounds for experimentation in this field.
Lim
Wilber
W
Department of Physics, Faculty of Science, National University of Singapore, Singapore 117542. choonsiang.lim@yale.edu
Feng
Yuan Ping
YP
D003198:Computer Simulation; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D043542:DNA, Z-Form / Q000032:analysis / Q000737:chemistry*; D004548:Elasticity; D008956:Models, Chemical; D008958:Models, Molecular; D009038:Motion; D009690:Nucleic Acid Conformation; D013314:Stress, Mechanical; D019415:Torque
D043542:DNA, Z-Form; D004247:DNA