title: Glucose sensor based on glucose oxidase immobilized by zirconium phosphate.
abstract: Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. Reportedly, alpha-ZrP is one of the candidates that are expected to improve the stability of enzymes immobilized on solid surfaces. We intercalated GOxs into ZrP (GOx/ZrP), cast the GOx/ZrP suspension in polyvinylalcohol on a platinum electrode, and dried it in a vacuum oven. The morphological layered structure was investigated by scanning electron microscopy. The enzymatic activities, which were determined by open-circuit potentiometric technique, reached the highest when GOxs were immobilized in ZrP at ca. pH 5. In vitro tests showed good linear responses in the 0-25 mM range and the sensitivity of 0.14 nA mM(-1) at 0.4 V vs. Ag/AgCl. The sensors, as made, were stable for more than 3 days within a limited deterioration.
authors.affiliation: Department of Chemistry, Sungshin Women's University, 249-1, Dongsun-dong, Seongbuk-gu, Seoul 136-742, Korea.
authors.forename: Sejin
authors.initials: S
authors.lastname: Park
authors.forename: Taek Dong
authors.initials: TD
authors.lastname: Chung
authors.forename: Sun Kil
authors.initials: SK
authors.lastname: Kang
authors.forename: Ran-A
authors.initials: RA
authors.lastname: Jeong
authors.forename: Hankil
authors.initials: H
authors.lastname: Boo
authors.forename: Hee Chan
authors.initials: HC
authors.lastname: Kim
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D010984:Platinum / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012680:Sensitivity and Specificity; D015040:Zirconium / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; C027006:zirconium phosphate; D010984:Platinum; D011142:Polyvinyl Alcohol; D015040:Zirconium; D005949:Glucose Oxidase; D005947:Glucose