Novel biodegradable cholesterol-modified polyrotaxane hydrogels for cartilage regeneration.
Cholesterol was introduced to a hydrolyzable polyrotaxane (PRx), not only to improve cell proliferation and glycosaminoglycan (GAG) production, but also to control the degradation rate of the hydrogels. The cholesterol was introduced to hydrolyzable PRx species by threading many alpha-cyclodextrins (alpha-CDs) on a poly(ethylene glycol) (PEG) chain having hydrolyzable ester linkages at the terminals; the PRx species were then cross-linked with other PEGs to prepare cholesterol-modified PRx hydrogels. The degree of cholesterol substitution was varied in the range of 1-25%. These hydrogels were examined to clarify the effect of cholesterol groups on mechanical properties, erosion time and chondrocyte proliferation. Highly porous biodegradable cholesterol-modified PRx hydrogels were fabricated using a combination of potassium hydrogen carbonate (as an effervescent salt) and citric acid. This fabrication process enabled the homogeneous expansion of pores within the polymer matrices, leading to well-interconnected macroporous hydrogels with a mean pore size of around 200-400 microm, ideal for high-density chondrocyte seeding. Time to complete degradation of the hydrogels was shortened by increasing the degree of substitution due to the aggregation of alpha-CDs through hydrophobic interaction of cholesterol groups. The presence of approx. 10% cholesterol improved the chondrocyte proliferation and GAG production. The modification of cholesterols to PRx is a good approach for creating new biodegradable hydrogels in terms of chondrocyte culture and controlling degradation time of the hydrogels.
Tachaboonyakiat
Wanpen
W
Innovation Plaza Ishikawa, Japan Science and Technology Agency, 2-13 Asahidai, Tatsunokuchi, Ishikawa 923-1211, Japan.
Furubayashi
Tomoyuki
T
Katoh
Masakazu
M
Ooya
Tooru
T
Yui
Nobuhiko
N
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002784:Cholesterol / Q000737:chemistry*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D003505:Cyclodextrins / Q000737:chemistry*; D004548:Elasticity; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D020442:Poloxamer / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D012038:Regeneration; D043862:Rotaxanes / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D003505:Cyclodextrins; D006025:Glycosaminoglycans; D020100:Hydrogels; D043862:Rotaxanes; C020341:glucosaminoglycans; C475673:polyrotaxane; D014867:Water; D020442:Poloxamer; D002784:Cholesterol