title: Unilateral kidney-selective gene transfer following the administration of naked plasmid DNA to the kidney surface in mice.
abstract: We developed a gene transfer following the administration of naked plasmid DNA (pDNA) to the kidney surface in mice, and found that the luciferase levels produced in the applied kidney were significantly higher than those produced in another kidney. In contrast, stable renal gene expression was not observed in the case of intraperitoneal or intravenous administration of pDNA. The level of gene expression after instillation of pDNA to the kidney surface reached maximum at 12 h and gradually diminished thereafter. The production of luciferase was saturated at 5 microg of pDNA, and was not affected by instillation volume. Furthermore, pDNA uptake from the kidney surface was proved by in situ experiments using a glass-made diffusion cell. We demonstrated a novel unilateral kidney-selective gene transfer following the administration of naked pDNA to the kidney surface in mice.
authors.affiliation: Graduate School of Biomedical Sciences, Nagasaki University, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Hirayama
authors.forename: Koyo
authors.initials: K
authors.lastname: Nishida
authors.forename: Shintaro
authors.initials: S
authors.lastname: Fumoto
authors.forename: Mikiro
authors.initials: M
authors.lastname: Nakashima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Junzo
authors.initials: J
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D018014:Gene Transfer Techniques; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage*
chemical_list: D019444:Vaccines, DNA