title: A physiological role for tRNA nucleotidyltransferase during bacteriophage infection.
abstract: Bacteriophage infection of E. coli cells deficient in the enzyme tRNA nucleotidyltransferase (cca mutants) resulted in greatly decreased production of viable progeny phage compared to wild type cells. This decrease amounted to as much as 90% in the case of T-even bacteriophages, and 50-65% for T-odd bacteriophages. However, infection by the RNA phages, Qbeta and f2, was unaffected by the cca mutation. Examination of T4 infection of cca hosts indicated that phage development proceeded normally, that near-normal numbers of progeny particles were formed, but that most of these particles were non-viable. Possible functions for E. coli tRNA nucleotidyltransferase during bacteriophage infection are discussed.
authors.affiliation: Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Morse
authors.forename: M P
authors.initials: MP
authors.lastname: Deutscher
mesh_terms: D017122:Bacteriophage T4 / Q000254:growth & development / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D012316:RNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D012316:RNA Nucleotidyltransferases; C023198:tRNA nucleotidyltransferase