Rapid detection of methicillin resistance in teicoplanin-resistant coagulase-negative staphylococci by a penicillin-binding protein 2' latex agglutination method.
Thirty-five clinical isolates of coagulase-negative staphylococci with decreased glycopeptide sensitivity were examined by a penicillin-binding protein (PBP2') latex agglutination (LA) test and were compared to the detection of the mecA gene by PCR, and oxacillin susceptibility determined minimum inhibitory concentrations. The latex test demonstrated high sensitivity and specificity for detecting methicillin resistance in coagulase-negative staphylococci after PBP2' induction with oxacillin.
Knausz
Márta
M
Clinical Microbiology, Aladár Petz County Teaching Hospital, 13 Zrinyi Street, 9024, Györ, Hungary. m.knausz@axelero.hu
Ghidán
Agoston
A
Grossato
Anna
A
Rozgonyi
Ferenc
F
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods; D016106:Methicillin Resistance / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D041162:Staphylococcus haemolyticus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D017334:Teicoplanin / Q000494:pharmacology*
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C487495:MecA protein, Staphylococcus epidermidis; D046915:Penicillin-Binding Proteins; C071437:VanA ligase, Bacteria; D017334:Teicoplanin; D019729:Carbon-Oxygen Ligases