[Application of GFP mark on inbred depuration of transgenic medaka (Oryzias latipes)].
A new expression vector with the promoter of the medaka (Oryzias latipes) beta-Actin promoter and green-fluorescent protein (GFP) gene was constructed based on Nde I site of pBluescript SK+.Microinjection experimentation proved that both expression rate of marker gene and the survival rate of transgenic embryos are high. The fourth generation of transgenic medaka,inbred variety,was obtained by heterozygote (F2 and F3) breeding of two generations and homozygote (F4) breeding of one generation based on mosaic (F1) of transgenic medaka. The detection results of stereo-fluorescence microscope show that green-fluorescent protein (GFP) gene is a kind of ideal marker gene.
Long
Hua
H
Key Laboratory of Freshwater Fish Germplasm Resources & Biotechnology of Agriculture Ministry, Yangtze River Fisheries Institute, Chinese Academy of Fisheries Sciences, Jingzhou, Hubei 434000,China. longhua0000@yahoo.com.cn
Muxia
Zheng-Ren
ZR