Techniques of image analysis for quantitative immunohistochemistry.
The aim of this paper was to evaluate the usefulness of digital image analysis techniques to measure the amount and strength of immunohistochemical markers. The new method, based on the spatial visualization technique, was confronted with methods of colour sampling and grey scale thresholding. Examples of applications of the techniques for apoptosis and proliferation markers are also presented.
Kaczmarek
E
E
Laboratory of Morphometry and Medical Image Processing, University of Medical Sciences, Poznań, Poland. elka@amp.edu.pl
Górna
A
A
Majewski
P
P
D015415:Biomarkers / Q000032:analysis; D006042:Goiter / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods*; D019394:Ki-67 Antigen / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
D015415:Biomarkers; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen