title: Catalytic system of the reactive oxygen species on the C60 fullerene basis.
abstract: OBJECTIVE
  To study the ability of fullerenes C(60) to catalyse the reactions of generation of reactive oxygen species (ROS) in water solution after photoexcitation and to affect the vitality of tumor cells in vitro.
  
  
  METHODS
  The number and vitality of cultured Ehrlich carcinoma cells or rat thymocytes were determined using tripane blue, ROS levels were registered using the methods of electron paramagnetic resonance (EPR) spectroscopy and spin traps, photoirradiation of water solution of fullerenes C(60) with visible light was carried out using mercury lamp.
  
  
  RESULTS
  Irradiation of water solution of fullerenes C(60) (10(-5) M) was accompanied with generation of ROS with the rate of 10 nMol/ml/min. After addition of irradiated C(60) solution to suspension of thymocytes or ascite cells, the decrease of the number of vital cells by 67 and 58%, respectively, has been registered.
  
  
  CONCLUSIONS
  Photoirradiated water solutions of fullerenes C(60) may be potentially useful for photodynamic therapy of tumors as ROS catalytic system.
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine.
authors.forename: Anatolij P
authors.initials: AP
authors.lastname: Burlaka
authors.forename: Yevgeniy P
authors.initials: YP
authors.lastname: Sidorik
authors.forename: Svitlana V
authors.initials: SV
authors.lastname: Prylutska
authors.forename: Olga P
authors.initials: OP
authors.lastname: Matyshevska
authors.forename: Olexandr A
authors.initials: OA
authors.lastname: Golub
authors.forename: Yurij I
authors.initials: YI
authors.lastname: Prylutskyy
authors.forename: Peter
authors.initials: P
authors.lastname: Scharff
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D002384:Catalysis; D004578:Electron Spin Resonance Spectroscopy; D037741:Fullerenes / Q000494:pharmacology*; D008027:Light; D010777:Photochemistry; D010778:Photochemotherapy / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D037741:Fullerenes; D017382:Reactive Oxygen Species; D014867:Water; C069837:fullerene C60