Study of mRNA expression of Arabidopsis thaliana under stimulation using modified differential display RT-PCR with silver staining.
We studied differential expressions of Arabidopsis thaliana seedlings under sound stimulation using modified differential display RT-PCR with silver staining in this paper. Six differentially expressed cDNA fragments were obtained. Molecular weight of fragments was between 200 and 600 bp, respectively. On the base of the experimentation, we considered sound stress would have been a positive or negative influence on plant growth. The experimental results indicated some genes was activated, meanwhile some were restrained under after stimulation under sound stress.
Bochu
Wang
W
Key Lab for Biomechanics and Tissue Engineering under the State Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China. wangbc2000@126.com
Jin
Zhang
Z
Biao
Li
L
Daohong
Wang
W
Chuanren
Duan
D
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D018749:RNA, Plant / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016622:Silver Staining / Q000379:methods*; D014508:Urea / Q000494:pharmacology
D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D018749:RNA, Plant; D012313:RNA; D014508:Urea; D004247:DNA