Discrepancies and interpretation problems in susceptibility testing of VIM-1-producing Klebsiella pneumoniae isolates.
Susceptibilities to beta-lactam antibiotics of five VIM-1-producing Klebsiella pneumoniae isolates were determined by broth microdilution, Etest, disk diffusion, and the automated systems Vitek 2, Phoenix, and MicroScan. Significant discrepancies were observed in the determination of susceptibility to imipenem and meropenem. Interpretation problems by the automated systems were also noted.
Giakkoupi
P
P
Department of Microbiology, National School of Public Health, 196 Alexandras Ave., Athens 11521, Greece.
Tzouvelekis
L S
LS
Daikos
G L
GL
Miriagou
V
V
Petrikkos
G
G
Legakis
N J
NJ
Vatopoulos
A C
AC
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology; D000077731:Meropenem; D008826:Microbial Sensitivity Tests / Q000379:methods; D011786:Quality Control; D015203:Reproducibility of Results; D013845:Thienamycins / Q000494:pharmacology*; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D013845:Thienamycins; D047090:beta-Lactams; D015378:Imipenem; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases; D000077731:Meropenem