Use of bioinformatics and PCR in the search for ABC transporter homology among various bacteria.
Bioinformatics databases and search tools are utilised to produce polymerase chain reaction (PCR) primers for the amplification of an ABC transporter gene from the clinically important anaerobe Finegoldia magna. On sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of Enterococcus faecalis. Little sequence data is available for F. magna and the newly isolated DNA could be a useful tool in the identification of this organism in clinical specimens.
Jauhangeer
B R
BR
Molecular and Medical Microbiology Research Group, School of Biosciences, University of Westminster, London, UK. jauhanb@wmin.ac.uk
Wren
M W D
MW
MacDonald
R A C
RA
Perry
D
D
Greenwell
R
R
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016208:Databases, Factual; D006801:Humans; D010462:Peptostreptococcus / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid
D018528:ATP-Binding Cassette Transporters; D004269:DNA, Bacterial