Microquantitative determination of hesperidin by pulse perturbation of the oscillatory reaction system.
A simple and reliable kinetic method for the determination of hesperidin (Hesp) is developed. It is based on potentiometric monitoring of the concentration perturbations of the matrix reaction system which is in a stable non-equilibrium stationary state close to the bifurcation point. The Bray-Liebhafsky oscillatory reaction is used as the matrix system. The response of the matrix to perturbations by different concentrations of Hesp is followed by using a Pt electrode. A linear relationship between maximal potential shift and the logarithm of Hesp concentrations is obtained between 7.5 and 599.4 microg mL-1. The limit of detection is 0.65 microg mL-1. The described procedure has been successfully applied to the determination of Hesp from different sources (capsules, industrial and hand-squeezed orange juice, and white wine).
Pejić
Natasa
N
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000, Belgrade, Serbia and Montenegro. bimesel@eunet.yu
Blagojević
Slavica
S
Anić
Slobodan
S
Vukojević
Vladana
V
Kolar-Anić
Ljiljana
L
D006569:Hesperidin / Q000032:analysis*; D008832:Microchemistry / Q000379:methods; D009991:Oscillometry; D012680:Sensitivity and Specificity
D006569:Hesperidin