title: [Different enzymatic activities recruitment by specific domains of TIF2 are involved in NF-kappaB transactivation].
abstract: We have previously shown that nuclear receptor coactivator overexpression significantly enhanced NF-kappaB activity in a dose response manner. We studied the mechanism by which TIF2 regulates NF-kappaB activity. We determined that: 1) the p38 specific inhibitor reduces 50% NF-kappaB transcriptional activity, even in cells that overexpress distinct TIF2 deletions; 2) there is a physical interaction between TIF2 and p38 and RelA determined through in vitro translated protein binding assays; 3) TIF2 is a p38 substrate; 4) there is a physical interaction between TIF2 and IKK in TNF-alpha 20 ng/ml stimulated or not HEK 293 cell protein extract, and IkappaB only in basal conditions, determined by binding pull down assays. This NF-kappaB complex regulates its activity and targets gene expression in a determined physiologic context depending on the coactivator complex content.
authors.affiliation: Instituto de Investigaciones Médicas Alfredo Lanari, Buenos Aires, Argentina.
authors.forename: Ignacio M
authors.initials: IM
authors.lastname: Nojek
authors.forename: Santiago E
authors.initials: SE
authors.lastname: Werbajh
authors.forename: Georgina P
authors.initials: GP
authors.lastname: Colo
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Rubio
authors.forename: Lorena D
authors.initials: LD
authors.lastname: Franco
authors.forename: Victor E
authors.initials: VE
authors.lastname: Nahmod
authors.forename: Monica A
authors.initials: MA
authors.lastname: Costas
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D051958:Nuclear Receptor Coactivator 2; D010766:Phosphorylation; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: C498001:NCOA2 protein, human; D016328:NF-kappa B; D051958:Nuclear Receptor Coactivator 2; D014157:Transcription Factors; D048051:p38 Mitogen-Activated Protein Kinases