Effect of prolactin on release of selected enzymes from the isolated rabbit liver.
This report analyzes the effect of prolactin (PRL) on ALT and AST release from the rabbit liver into the preservation solution. Dissected and perfused livers were stored for 24 hours in Ringer's solution without (control group) or with PRL (experimental group). During the organ preservation, sample of the solution were obtained at 1, 8, 12, 16, 18, and 24 hours. It was found that PRL added to Ringer's solution significantly decreased the quantity and rate of released ALT (P < .081) and AST (P < .029) from the preserved liver. ALT was released 2.51 times more slowly (kappa = -0.03329 [h(-1)]) and AST 3.43 times more slowly (kappa = -0.08356 [h(-1)]) into Ringer's solution with PRL. The experimental group showed maintenance of the value of the de Ritis index at a stable level between 2.0-3.0. In conclusion, PRL added to a preservation solution significantly decreased the quantity and slowed the release rate of ALT and AST aminotransferases from the preserved rabbit liver, implying that this hormone has hepatoprotective properties.
Ryszka
F
F
Department of Applied Pharmacy and Drug Technology, Medical University of Silesia, Sosnowiec, Poland.
Dolińska
B
B
Sławski
M
M
Orkisz
W
W
D000410:Alanine Transaminase / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods; D011388:Prolactin / Q000494:pharmacology*; D011817:Rabbits
D011388:Prolactin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase