title: Pregnancy-associated plasma protein A during hemodialysis with polyamide and diacetate cellulosic membranes.
abstract: Pregnancy-associated plasma protein A (PAPP-A) is a new prognostic factor of acute coronary syndrome in the general population. It is elevated in hemodialysis (HD) patients and at baseline, it was shown to be related to inflammation and oxidative stress. The aim of the study was to examine the relationship of PAPP-A and oxidative stress and inflammatory markers to HD treatment. Studied parameters were determined in 10 chronic HD patients treated with low flux polyamide (1st session) and diacetate cellulosic membranes (2nd session) at the beginning, after 15 minutes and at the end of the dialysis session. TRACE method (Time Resolved Amplified Cryptate Emission) was used for PAPP-A assessment. Results were evaluated with ANOVA. PAPP-A levels did not depend on the type of HD membrane but changed significantly with the time of the HD session. They increased significantly from the beginning of HD to 15 min and then decreased to the end of the HD session - p<0.05 15 min of HD vs start, p<0.01 end vs start, p<0.0001 end vs 15 min of HD for polyamide membrane and p=0.05 15 min of HD vs start, p<0.01 end vs start, p<0.0001 end vs 15 min of HD for diacetate cellulosic membrane. Changes in other parameters and differences between membranes were only minimal. We can conclude that PAPP-A as a marker of cardiovascular damage shows significant changes during the HD session. Its initial increase might be ascribed to its release from complexes or storage. During dialysis, it might be destroyed or cleaved and removed as free fragments. Its levels both before and after the HD session are higher than in healthy subjects.
authors.affiliation: Institute of Medical Biochemistry, Department of Nephrology, 1st Faculty of Medicine and General University Hospital, Charles University, Prague-Czech Republic. marta.kalousova@seznam.cz
authors.forename: M
authors.initials: M
authors.lastname: Kalousová
authors.forename: J
authors.initials: J
authors.lastname: Lachmanová
authors.forename: M
authors.initials: M
authors.lastname: Mokrejsová
authors.forename: B
authors.initials: B
authors.lastname: Míková
authors.forename: L
authors.initials: L
authors.lastname: Fialová
authors.forename: I M
authors.initials: IM
authors.lastname: Malbohan
authors.forename: V
authors.initials: V
authors.lastname: Tesar
authors.forename: T
authors.initials: T
authors.lastname: Zimá
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000175:diagnosis* / Q000209:etiology / Q000401:mortality; D002482:Cellulose / Q000494:pharmacology*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000175:diagnosis / Q000401:mortality / Q000628:therapy*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009757:Nylons / Q000494:pharmacology*; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis / Q000378:metabolism*; D011336:Probability; D011379:Prognosis; D011446:Prospective Studies; D006435:Renal Dialysis / Q000009:adverse effects / Q000379:methods*; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D008567:Membranes, Artificial; D009757:Nylons; D002482:Cellulose; D011266:Pregnancy-Associated Plasma Protein-A