Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5'-flanking region.
The results presented demonstrate the expression of pfkfb4 gene in adult testis and in a mouse spermatogonia germ cell line (GC-1spg). The genomic organization of the human pfkfb4 gene shows the existence of 14 exons and 13 introns, spanning 45 kb. A detailed analysis of the 5'-flanking region by transient transfection assays with different 5'-deletion promoter constructs in GC-1spg and mouse sertoli cells (TM-4), allows us to define the minimal promoter unit, containing several GC-rich and ETF sequences along the first -141 nucleotides involved in basal expression. This gene is activated by serum and chemical hypoxia (CoCl(2) treatment) whereas beta-estradiol decreases its expression.
Gómez
Marta
M
Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Campus de Bellvitge, Universitat de Barcelona, Feixa Llarga s/n E-08907 L'Hospitalet, Spain.
Manzano
Anna
A
Navarro-Sabaté
Aurea
A
Duran
Joan
J
Obach
Mercè
M
Perales
Jose C
JC
Bartrons
Ramon
R
D024506:5' Flanking Region / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005091:Exons / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D005854:Germ Cells / Q000378:metabolism; D006801:Humans; D007438:Introns / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D025481:Phosphofructokinase-2 / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017384:Sequence Deletion / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D013737:Testis / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution
D012333:RNA, Messenger; D008156:Luciferases; D025481:Phosphofructokinase-2