Clonality analysis of cell lineages in acute myeloid leukemia with inversion 16.
Bone marrow from 5 patients with acute myeloid leukemia with inversion 16, inv(16), was studied by a combination of fluorescence activated cell sorting (FACS) and fluorescence in situ hybridization (FISH) to establish the extent of cell-lineage involvement of inv(16). In all cases, interphase FISH demonstrated the inv(16) in 80-92% of blasts, monocytes, granulocytes, and a proportion of B-cells (21-41%). In contrast, inv(16) was not detectable above threshold levels in mature T-cells. The current study provides a direct evidence for the clonal involvement of myeloid lineage cells and B-lymphocytes and suggests that T-cells are not part of the malignant clone in this disease.
Chang
Hong
H
Department of Laboratory Hematology, Princess Margaret Hospital, University Health Network, 610 University Avenue, 4-320 Toronto, ON, M5G 2M9, Canada. hong.chang@uhn.on.ca
Nayar
Rakash
R
Li
Dan
D
Sutherland
D Robert
DR
D000208:Acute Disease; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000473:pathology; D007446:Chromosome Inversion / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000473:pathology