Evidence for mutual exclusion in the tryptic hydrolysis of two peptide bonds in maleylated trypsinogen.
Treatment of completely maleylated trypsinogen with trypsin resulted in a mutual exclusion of hydrolysis of the two arginyl peptide bonds wherein one half of the molecules of the modified zymogen were cleaved at the Arg55-Leu56 bond and the other half of the molecules were cleaved at the Arg105- Val106 bond and no molecules were cleaved at both arginyl bonds.
Grooms
T A
TA
Department of Biological Chemistry, University of Cincinnati, College of Medicine, 45267 Cincinnati, Ohio, USA.
Ogle
J D
JD
D006868:Hydrolysis; D008299:Maleic Anhydrides / Q000737:chemistry*; D012972:Sodium Hydroxide; D013379:Substrate Specificity; D017186:Titrimetry; D014357:Trypsin / Q000378:metabolism*; D014362:Trypsinogen / Q000737:chemistry* / Q000378:metabolism*
D008299:Maleic Anhydrides; D012972:Sodium Hydroxide; D014362:Trypsinogen; D014357:Trypsin