The effects of dimethyl sulfoxide on liver damage caused by ischemia-reperfusion.
BACKGROUND
The aim of this study was to investigate the effects of dimethyl sulfoxide on liver damage caused by ischemia-reperfusion after portal vein clamping.


METHODS
Forty New Zealand rabbits were divided into three groups with the portal veins of all the rabbits except the sham group clamped for 30 minutes: group I, sham procedure; group II, control group; and group III, 500 mg/kg DMSO. The drug was administered IM in the left inguinal region 30 minutes before the operation. Blood samples (5 mL) were taken from the animals at 15, 30, and 45 minutes. At the end of the experiment 1 g of liver tissue samples were obtained. Malondialdhyde (MDA), nitric oxide (NO), AST, ALT, and LDH plasma levels were measured in the blood samples. Liver tissue samples stained with hematoxylin eosin were examined under light microscopy for histopathological changes.


RESULTS
The liver enzymes in both clamping groups increased significantly compared with the sham group (P < .01). Enzyme levels of the DMSO group decreased significantly compared to the control clamping group (P < .05). Similar to the enzyme changes, MDA and NO levels increased in the portal vein clamping versus the sham group and decreased in the drug-administered group versus the control clamped group (P < .03). The severity of histopathological changes was less in the DMSO group than in the clamped controls.


CONCLUSIONS
DMSO decreased the severity of liver damage after portal vein clamping.
Sahin
M
M
Department of General Surgery, Selçuk University, Medical Faculty, Konya, Turkey.
Avsar
F M
FM
Ozel
H
H
Topaloglu
S
S
Yilmaz
B
B
Pasaoglu
H
H
Avunduk
M C
MC
Erikoglu
M
M
Hengirmen
S
S
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004195:Disease Models, Animal; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood / Q000201:enzymology / Q000473:pathology*
D009569:Nitric Oxide; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D004121:Dimethyl Sulfoxide