title: Noninvasive mechanical ventilation of mice by using a balloon-tipped cannula.
abstract: Cardiovascular research in mouse models can lead to respiratory insufficiency with the need for temporary mechanical ventilation to reduce mortality. We developed a noninvasive method for brief ventilation in which a balloon-tipped cannula is inserted into the oral cavity of the mouse. The rubber balloon is constructed in such a way that it becomes inflated when the pressure in the tube rises. We tested the cannula in ten C57BL/6J mice which underwent mechanical ventilation by a membrane-pump-driven ventilator. Results of blood gas analyses in the ten cannulated animals were in the normal range after 1 h of mechanical ventilation and did not differ significantly from those of anesthetized and spontaneously breathing mice (n = 5) and mice mechanically ventilated via tracheal intubation (n = 5). Noninvasive mechanical ventilation by using the balloon-tipped cannula is a useful method which is quick and easy to manipulate.
authors.affiliation: Department of Anesthesiology, University of Saarland, D-66421 Homburg/Saar, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Winning
authors.forename: Hanno
authors.initials: H
authors.lastname: Huwer
authors.forename: Jasmin
authors.initials: J
authors.lastname: Redjai
authors.forename: Christian
authors.initials: C
authors.lastname: Naumann
authors.forename: Ilka
authors.initials: I
authors.lastname: Winning
authors.forename: Gunther
authors.initials: G
authors.lastname: Schneider
authors.forename: Michael
authors.initials: M
authors.lastname: Bauer
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis / Q000662:veterinary; D002404:Catheterization / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D007442:Intubation, Intratracheal / Q000662:veterinary; D008279:Magnetic Resonance Imaging / Q000662:veterinary; D051379:Mice; D008810:Mice, Inbred C57BL; D009055:Mouth; D012121:Respiration, Artificial / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D014730:Veterinary Medicine / Q000379:methods*