[Distribution of the HIV/AIDS associated CCR5Delta32,CCR2b-64I,SDF1-3'A allelesin Chinese Dai and Chingpaw populations from Dehong autonomous prefecture of Yunnan Province].
The purpose of the work is to investigate the frequencies and polymorphisms of HIV-1 resistant CCR5delta32,CCR2b-64I,SDF1-3'A alleles in Chinese Dai and Chingpaw populations. Whole blood samples from 101 Dai subjects and 113 Chingpaw were collected randomly and their genomic DNA were extracted with QIAgen Blood Kits. Allelic frequencies were identified by PCR-RFLP analysis. Allelic polymorphisms in Dai population or Chingpaw population and both sexes in the samples were analyzed by chi(2) test. The frequencies of CCR5delta32,CCR2b-64I,SDF1-3'A alleles in Dai population were 0.0000,0.2130,0.2030, respectively. The frequencies of CCR5delta32,CCR2b-64I,SDF1-3'A alleles in Chingpaw population were 0.000, 0.1637, 0.1770, respectively. Distributions of the CCR2b-64I,SDF1-3'A alleles among the both populations were in accordance with Hardy-Weinberg equilibrium. No statistical difference was found in the allelic frequencies of both CCR2b-64I and SDF1-3'A between male and female individuals. The frequencies of CCR5delta32,CCR2b-64I alleles in Chinese Dai and Chingpaw populations are similar to that in Chinese Han population, while the frequency of SDF1-3'A allele in Chinese Dai and Chingpaw populations are lower in contrast to that in Chinese Han population. The genotyping and polymorphism of CCR5delta32,CCR2b-64I,SDF1-3'A alleles in Chinese Dai and Chingpaw populations of Yunnan Province are the first time studied in China. The significance of the three mutant alleles conferring genetic resistance to HIV-1 and AIDS progression remains to be clarified.
Ye
Jun-Jie
JJ
Institute of Family Planning of Yunnan Province, Kunming, 650021, China. yejunjie111@sina.com
Wang
Fu-Sheng
FS
Peng
Lin
L
Jin
Lei
L
Ding
Ming
M
Liu
Ming-Xu
MX