title: Occurrence, characterisation and detection of potential virulence determinants of emerging aquatic bacterial pathogens from the Philippines and Thailand.
abstract: Strains of Aeromonas spp., 'non-cholera vibrios' (NCVs) and Plesiomonas shigelloides isolated from aquatic environments, fish and human diarrhoeal cases in the Philippines and Thailand were characterised for potential virulence markers. Thus, the production of cytotoxin, cell-associated and cell-free haemolysin and their capacity to adhere to human intestinal (Henle 407) cells in vitro was investigated. In addition, the occurrence of tlh and tdh haemolysin genes and urease activity among V. parahaemolyticus strains was investigated. The results showed that strains recovered from clinical sources (human and fish) produced these virulence factors, whereas these are absent in environmental strains.
authors.affiliation: Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna, Philippines 4031 [corrected]
authors.forename: R P
authors.initials: RP
authors.lastname: Maluping
authors.forename: C R
authors.initials: CR
authors.lastname: Lavilla-Pitogo
authors.forename: A
authors.initials: A
authors.lastname: DePaola
authors.forename: J M
authors.initials: JM
authors.lastname: Janda
authors.forename: K
authors.initials: K
authors.lastname: Krovacek
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D000818:Animals; D001422:Bacterial Adhesion; D003603:Cytotoxins / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology*; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology* / Q000662:veterinary; D006460:Hemolysin Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D010679:Philippines; D016981:Plesiomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D013785:Thailand; D014510:Urease / Q000032:analysis; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000032:analysis*; D014871:Water Microbiology
chemical_list: D003603:Cytotoxins; D006460:Hemolysin Proteins; D037521:Virulence Factors; D014510:Urease