[A simple and effective method for extracting total RNA from domestic fungus].
With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols, polymeric carbohydrates and proteoglycans. Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol, sodium acetate were used to precipitate RNA selectively. Pure and intact RNA can be effectively prepared by this method.
Zhou
Kai-Song
KS
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Xue
Jiu-Gang
JG
Chang
Ning
N
Chen
Chang
C
Zhang
Han-Xing
HX
Zhang
Chang-Kai
CK