title: Molecular characterisation of Trypanosoma rangeli strains isolated from Rhodnius ecuadoriensis in Peru, R. colombiensis in Colombia and R. pallescens in Panama, supports a co-evolutionary association between parasites and vectors.
abstract: We present data on the molecular characterisation of strains of Trypanosoma rangeli isolated from naturally infected Rhodnius ecuadoriensis in Peru, from Rhodnius colombiensis, Rhodnius pallescens and Rhodnius prolixus in Colombia, and from Rhodnius pallescens in Panama. Strain characterisation involved a duplex PCR with S35/S36/KP1L primers. Mini-exon gene analysis was also carried out using TrINT-1/TrINT-2 oligonucleotides. kDNA and mini-exon amplification indicated dimorphism within both DNA sequences: (i) KP1, KP2 and KP3 or (ii) KP2 and KP3 products for kDNA, and 380 bp or 340 bp products for the mini-exon. All T. rangeli strains isolated from R. prolixus presented KP1, KP2 and KP3 products with the 340 bp mini-exon product. By contrast, all T. rangeli strains isolated from R. ecuadoriensis, R. pallescens and R. colombiensis, presented profiles with KP2 and KP3 kDNA products and the 380 bp mini-exon product. Combined with other studies, these results provide evidence of co-evolution of T. rangeli strains associated with different Rhodnius species groups east and west of the Andean mountains.
authors.affiliation: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Ibagué, Colombia.
authors.forename: D A
authors.initials: DA
authors.lastname: Urrea
authors.forename: J C
authors.initials: JC
authors.lastname: Carranza
authors.forename: C A Cuba
authors.initials: CA
authors.lastname: Cuba
authors.forename: R
authors.initials: R
authors.lastname: Gurgel-Gonçalves
authors.forename: F
authors.initials: F
authors.lastname: Guhl
authors.forename: C J
authors.initials: CJ
authors.lastname: Schofield
authors.forename: O
authors.initials: O
authors.lastname: Triana
authors.forename: G A
authors.initials: GA
authors.lastname: Vallejo
mesh_terms: D000818:Animals; D003105:Colombia; D021901:DNA, Intergenic / Q000235:genetics; D018105:DNA, Kinetoplast / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D006790:Host-Parasite Interactions / Q000235:genetics; D010176:Panama; D010568:Peru; D010802:Phylogeny; D012239:Rhodnius / Q000469:parasitology*; D014345:Trypanosoma / Q000145:classification / Q000235:genetics*
chemical_list: D021901:DNA, Intergenic; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan