title: Modeling of phosphate ion transfer to the surface of osteoblasts under normal gravity and simulated microgravity conditions.
abstract: We have modeled the transport and accumulation of phosphate ions at the remodeling site of a trabecular bone consisting of osteoclasts and osteoblasts situated adjacent to each other in straining flows. Two such flows are considered; one corresponds to shear levels representative of trabecular bone conditions at normal gravity, the other corresponds to shear level that is representative of microgravity conditions. The latter is evaluated indirectly using a simulated microgravity environment prevailing in a rotating wall vessel bioreactor (RWV) designed by NASA. By solving the hydrodynamic equations governing the particle motion in a RWV using a direct numerical simulation (DNS) technique, the shear stress values on the surface of the microcarriers are found. In our present species transfer model, osteoclasts release phosphate ions (Pi) among other ions at bone resorption sites. Some of the ions so released are absorbed by the osteoblast, some accumulate at the osteoblast surface, and the remainder are advected away. The consumption of Pi by osteoblasts is assumed to follow Michaelis-Menten (MM) kinetics aided by a NaPi cotransporter system. MM kinetics views the NaPi cotransporter as a system for transporting extracellular Pi into the osteoblast. Our results show, for the conditions investigated here, the net accumulation of phosphate ions at the osteoblast surface under simulated microgravity conditions is higher by as much as a factor of three. Such increased accumulation may lead to enhanced apoptosis and may help explain the increased bone loss observed under microgravity conditions.
authors.affiliation: Department of Mechanical Engineering and Applied Mechanics, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104-6315, USA.
authors.forename: Karthik
authors.initials: K
authors.lastname: Mukundakrishnan
authors.forename: Portonovo S
authors.initials: PS
authors.lastname: Ayyaswamy
authors.forename: Makarand
authors.initials: M
authors.lastname: Risbud
authors.forename: Howard H
authors.initials: HH
authors.lastname: Hu
authors.forename: Irving M
authors.initials: IM
authors.lastname: Shapiro
mesh_terms: D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D001692:Biological Transport; D001842:Bone and Bones / Q000502:physiology; D003198:Computer Simulation; D006112:Gravitation; D006801:Humans; D007477:Ions; D007700:Kinetics; D008962:Models, Theoretical; D010006:Osteoblasts / Q000378:metabolism*; D010710:Phosphates / Q000737:chemistry; D012399:Rotation; D013026:Space Flight; D014893:Weightlessness
chemical_list: D007477:Ions; D010710:Phosphates; C018279:sodium phosphate