title: [Study of polymorphism at new Y-STR DYS605 in a Chinese Han population of Shanxi].
abstract: We study the polymorphism at DYS605 ,a new tetranucleotide Y-STR locus,in a Chinese Han population of Shanxi to meet the need of more genetic markers in forensic practice and genetic analysis. DNA were extracted from 128 unrelated male venous blood, and amplified using GDB primers. PCR products were detected using non-denaturing polyacrylamide gel electrophoresis and silver staining. Five alleles named 22,21,20,19,18 were observed with frequency of 0.0156;0.1797;0.4531;0.2891;0.0625. PCR products were not found in female DNA. Using a long enough gel for a long electrophoresis time is strongly encouraged because the rung between allele 20 and allele19 is smaller than expected. Allele sequences show that the repetitive units of DYS605 were composed with the variant units and non-variant units.
authors.affiliation: School of Forensic Medicine, Shanxi Medical University, Taiyuan 030 001, China. zgengqian@163.com
authors.forename: Geng-Qian
authors.initials: GQ
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Zhang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Xu
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Xing
authors.forename: Ying-Yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Yun
mesh_terms: D000483:Alleles; D044466:Asian People; D002681:China / Q000208:ethnology; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic