[Recombinant human macrophage colony-stimulating factor expressed in silkworm accelerates hematopoietic recovery of radiolesion mice].
OBJECTIVE
To demonstrate the hematopoietic enhancement activity of recombinant human macrophage colony-stimulating factor (rhM-CSF).


METHODS
Balb/c mice were sublethally irradiated with 60Co gamma-rays and then the rhM-CSF expressed in silkworm (10(3)U per individual for 7 days) was administered intraperitoneally.


RESULTS
(1) The peripheral white blood cell counts of treatment group at day 15 and day 20 after irradiation were significantly higher than those of normal control group; (2) At days 10, 15, 20, 25 and 30 after irradiation, the difference between the peripheral monocyte counts of the treatment and control group were statistically significant; (3) At days 15, 20 and 25 after irradiation, the difference between the peripheral granulocyte counts of the two groups were also significant; (4) At day 9 after irradiation, the bone marrow CFU GM yield of the control group were significantly lower than those of the treatment group.


CONCLUSIONS
rhM-CSF expressed in silkworm could accelerate hematopoietic recovery in irradiated mice.
Ji
X
X
Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 210029.
Yao
K
K
Qin
J
J
Li
H
H
Sun
K
K
Zhu
D
D
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006098:Granulocytes / Q000187:drug effects / Q000528:radiation effects; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*
D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor