Comparative effects of high glucose on different adult sympathetic neurons in culture.
In vivo, diabetes causes neuropathy in the sympathetic celiac/superior mesenteric ganglion (CG/SMG) but not the superior cervical ganglion (SCG). Therefore, our aim was to compare the effects of high glucose in vitro on adult rat SCG and CG/SMG neurons. High glucose decreased viability and neurite outgrowth and increased TUNEL staining in freshly dissociated neurons from both ganglia. In cultures established for 24 h before exposure to high glucose, a significant (p < 0.01) decrease in neurite-bearing neurons from CG/SMG but not SCG occurred. CG/SMG neurons took longer (p < 0.05) to initiate neurite outgrowth and had lower (p < 0.002) anti-oxidant defence enzyme activity. These properties may contribute to the selective development of neuropathy in CG/SMG in diabetes in vivo.
Semra
Yemane K
YK
Autonomic Neuroscience Institute, Department of Anatomy and Developmental Biology, University College London, Royal Free Campus, Rowland Hill Street, London, NW3 2PF, UK.
Smith
Neil C E
NC
Lincoln
Jill
J
D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005728:Ganglia, Sympathetic / Q000166:cytology*; D005947:Glucose / Q000494:pharmacology*; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D008297:Male; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011419:Propidium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
D011419:Propidium; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase; D005947:Glucose