[Cloning of ZFY /ZFX gene of the tufted deer and identification of its sex].
According to the human sex differentiation related ZFY and ZFX genes, a pair of primers were designed , and fragments were amplified from the genomic DNA of male or female tufted deer. Subsequently the amplified fragments were cloned into the vector pMD18T and were sequenced. It is found that the sequences of ZFY gene and ZFX gene have 91% homology. Based on the different nucleotides, restriction site of Ava II was found to be specific to ZFX gene. The results show that the combination of PCR with Ava II digestion is a simple and sensitive way to identify the tufted deer sex.
Jiang
Hua-Yun
HY
College of Life Science, Nanjing Normal University, Nanjing 210 097, China. flowerjhy@sohu.com
Cao
Xiang-Rong
XR
Zhang
Xi-Ran
XR
Hu
Jun
J
Xu
Chun-Mao
CM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D003670:Deer / Q000235:genetics*; D005260:Female; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017385:Sequence Homology; D019849:Sex Determination Processes; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C054430:ZFY protein, human; C062076:zinc finger protein, X-linked