Quantitative expression of TEL in childhood acute lymphoblastic leukemia.
Loss of heterozygosity of chromosome 12p in human precursor B-cell ALL invariably results in loss of TEL coding sequences. Accompanied by a 12;21 translocation, such loss of heterozygosity ensures complete loss of the wild-type TEL. No inactivating mutations of the retained TEL allele have been reported in leukemias with hemizygous deletion. However, only minimal data reported the expression of the wild-type TEL in ALL. We now demonstrate that quantitative real-time RT-PCR from leukemic RNA samples could be indicative of compromised TEL expression in childhood ALL and therefore loss of TEL function.
Timson
G
G
King Fahad National Centre for Children's Cancer and Research, Riyadh, Saudi Arabia.
Gutiérrez
M I
MI
Bhatia
K
K
D000293:Adolescent; D000483:Alleles; D014408:Biomarkers, Tumor; D015139:Blotting, Southern; D002648:Child; D002675:Child, Preschool; D050676:Core Binding Factor Alpha 2 Subunit; D019175:DNA Methylation; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007223:Infant; D019656:Loss of Heterozygosity; D008297:Male; D009154:Mutation; D009687:Nuclear Proteins; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D050783:Proto-Oncogene Proteins c-ets; D012313:RNA / Q000378:metabolism; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D014178:Translocation, Genetic; D000097764:ETS Translocation Variant 6 Protein
D014408:Biomarkers, Tumor; D050676:Core Binding Factor Alpha 2 Subunit; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D015514:Oncogene Proteins, Fusion; D050783:Proto-Oncogene Proteins c-ets; D012313:RNA; D012097:Repressor Proteins; D000097764:ETS Translocation Variant 6 Protein; C102421:TEL-AML1 fusion protein