Effect of carotenoids and ascorbic acid of Japanese persimmons on cellular lipid peroxidation in HepG2 cells.
In this experiment, we examine the functional property of carotenoids; beta-cryptoxanthin (Cry), zeaxanthin (Zea), beta-carotene (Car)) and ascorbic acid (AsA). The accumulation amounts of Cry, Zea and Car in HepG2 cells cultured in the high concentration medium were larger than that in a low concentration. Further those accumulation amounts in long incubation time within 24 hours were greater than that in a shorter time. When the added carotenoid concentration, with or without hydrogen peroxide, increased from 0 to 5 microM in the culture medium, the thiobarbituric acid reaction substance (TBARS) values in the HepG2 cells decreased significantly (p < 0.05). The decrease of TBARS values shows the antioxidative property of the carotenoids. When AsA and Tocopherol(Toc) were added to the medium from 0 to 20 microM, the TBARS values, with or without hydrogen peroxide, decreased significantly with increasing concentrations of AsA and Toc respectively (p < 0.05). The decreased amount of TBARS in 5 microM Cry compared with control(0 microM) was the largest among 6 antioxidants (Cry, Car, Zea, Retinol(Ret), AsA, Toc) used in this experiment.
Hosotani
Keisuke
K
Faculty of Education, Wakayama University, Wakayama, Japan. hosotani@center.wakayama-u.ac.jp
Kawahata
Atsushi
A
Koyama
Kanako
K
Murakami
Chikako
C
Yoshida
Hiromi
H
Yamaji
Ryoichi
R
Inui
Hiroshi
H
Nakano
Yoshihisa
Y
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D001692:Biological Transport; D006528:Carcinoma, Hepatocellular; D002338:Carotenoids / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D045744:Cell Line, Tumor; D029788:Diospyros / Q000737:chemistry*; D006801:Humans; D007564:Japan; D015227:Lipid Peroxidation / Q000187:drug effects*; D008113:Liver Neoplasms; D010936:Plant Extracts / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014801:Vitamin A / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000494:pharmacology
D000975:Antioxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A; D002338:Carotenoids; D024502:alpha-Tocopherol; D001205:Ascorbic Acid