title: A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl- secretory pathways to cause diarrhea.
abstract: Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a human pathogen that causes diarrhea. We investigated the effects of ASH on Cl(-) transport in human colonic epithelial cells. ASH increased short-circuit currents (Isc) and (125)I efflux from Caco-2 cells, indicating ASH activate Cl(-) secretion. Additions of inhibitors of cyclic AMP dependent Cl(-) channels, glybenclamide and NPPB suppressed the Isc and (125)I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP-dependent Cl(-) secretory pathway could be related with diarrhea induced by A. sobria.
authors.affiliation: Department of Nutrition, School of Medicine, Tokushima University, 3-18-5 Kuramoto-cho, Tokushima City, Tokushima 770-8503, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Tanoue
authors.forename: Akira
authors.initials: A
authors.lastname: Takahashi
authors.forename: Keinosuke
authors.initials: K
authors.lastname: Okamoto
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Fujii
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Taketani
authors.forename: Nagakatsu
authors.initials: N
authors.lastname: Harada
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakano
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakaya
mesh_terms: D000333:Aeromonas / Q000737:chemistry*; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D018938:Caco-2 Cells; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D018118:Chloride Channels / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D003967:Diarrhea / Q000378:metabolism / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000633:toxicity*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D051379:Mice
chemical_list: D001427:Bacterial Toxins; D018118:Chloride Channels; D002712:Chlorides; D006460:Hemolysin Proteins; D000242:Cyclic AMP