title: Studies on the membrane interactions of the cyclotides kalata B1 and kalata B6 on model membrane systems by surface plasmon resonance.
abstract: In this study we have demonstrated the interactions of kalata B1 and its naturally occurring analogue kalata B6 with five model lipid membranes and have analyzed the binding kinetics using surface plasmon resonance. Two kalata peptides showed a higher affinity for the phosphatidylethanolamine-containing membranes, indicating that the peptides would bind selectively to bacterial membranes. Also we have optimized the procedure for the immobilization of five liposome mixtures and have shown that the procedure provides reproducible levels of immobilized liposomes and could be used to screen the selective binding of putative antimicrobial peptides to model mammalian or microbial phospholipid membranes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia. hiroshi.kamimori@shinonogi.co.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamimori
authors.forename: Kristopher
authors.initials: K
authors.lastname: Hall
authors.forename: David J
authors.initials: DJ
authors.lastname: Craik
authors.forename: Marie-Isabel
authors.initials: MI
authors.lastname: Aguilar
mesh_terms: D047169:Cyclotides / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D008567:Membranes, Artificial; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D020349:Surface Plasmon Resonance
chemical_list: D047169:Cyclotides; D008081:Liposomes; D008567:Membranes, Artificial; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C091930:kalata B1; C501913:kalata B6; C483858:phosphatidylethanolamine