Quantitative determination of urinary 8-hydroxy-2'-deoxyguanosine level in healthy Japanese volunteers.
8-hydroxy-2'-deoxyguanosine (8-OHdG), as a measure of oxidative stress, was measured in healthy Japanese volunteers using an ELISA (New 8-OHdG Check, JICA). Analysis of daytime spot urine of 83 healthy male subjects and smoking habit, exercise and age revealed significant correlation only between the urinary level of 8-OHdG and age. As the inter-individual variation of 8-OHdG of the daytime spot urine was relatively high, we next determined inter-and intra-individual variation of 5 healthy volunteers. The levels of 8-OHdG/creatinine in morning spot urine significantly correlated with 8-OHdG levels in 24-h pool urine. Thus, a morning spot urine sample can be used for the measurement of 8-OHdG instead of inconvenient 24-h sampling.
Miwa
Misao
M
National Food Research Institute, Tsukuba 305-8642, Japan. m3miwa@nodai.ac.jp
Matsumaru
Hiroyuki
H
Akimoto
Yukari
Y
Naito
Shigehiro
S
Ochi
Hirotomo
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000375:Aging / Q000502:physiology; D015415:Biomarkers / Q000652:urine; D003404:Creatinine / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D007564:Japan; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress; D012016:Reference Values; D012044:Regression Analysis
D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine