[The factors affecting the efficiency of mutiplex PCR].
In the research,the outputs of different cycle parameters, PCR buffer and reaction volumes are compared. The results indicated that the annealing temperature, annealing time, elongated time and ingredient of PCR buffer affected mutiplex PCR, but the reaction volume and cycle number had few effect on it.
Huang
Yin-Hua
YH
National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Hu
Xiao-Xiang
XX
Xu
Wei-Zhuo
WZ
Gao
Yu
Y
Feng
Ji-Dong
JD
Sun
Han
H
Li
Ning
N