A novel actinomycete strain de-replication approach based on the diversity of polyketide synthase and nonribosomal peptide synthetase biosynthetic pathways.
The actinomycetes traditionally represent one of the most important sources for the discovery of new metabolites with biological activity; and many of these are described as being produced by polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). We present a strain characterization system based on the metabolic potential of microbial strains by targeting these biosynthetic genes. After an initial evaluation of the existing bias derived from the PCR detection in a well defined biosynthetic systems, we developed a new fingerprinting approach based on the restriction analysis of these PKS and NRPS amplified sequences. This method was applied to study the distribution of PKS and NRPS biosynthetic systems in a collection of wild-type actinomycetes isolated from tropical soil samples that were evaluated for the production of antimicrobial activities. We discuss the application of this tool as an alternative characterization approach for actinomycetes and we comment on the relationship observed between the presence of PKS-I, PKS-II and NRPS sequences and the antimicrobial activities observed in some of the microbial groups tested.
Ayuso
Angel
A
Centro de Investigación Básica, Merck Sharp and Dohme de España S.A., Josefa Valcárcel 38, Madrid, 28027, Spain.
Clark
Desmond
D
González
Ignacio
I
Salazar
Oscar
O
Anderson
Annaliesa
A
Genilloud
Olga
O
D039903:Actinobacteria / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D016172:DNA Fingerprinting; D005798:Genes, Bacterial; D014644:Genetic Variation; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000096:biosynthesis / Q000235:genetics*; D048630:Polyketide Synthases / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D014329:Tropical Climate
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase