[Effects of hepatectomized rat serum on the transdifferentiation of adult rat bone marrow cells into hepatocyte-like cells].
OBJECTIVE
To study the effects of hepatectomized rat serum and hepatocyte growth factor (HGF) on the transdifferentiation of adult rat bone marrow stem cells (ABMSCs) into hepatic parenchymal cells.


METHODS
The serum was collected from the rats 24 hours after being subjected to subtotal hepatectomy. ABMSCs were collected and cultured in DMEM/F12 (1:1) containing the hepaetectomized rat serum or HGF. The differentiated hepatocyte-like cells were labeled with CM-DiI and administrated by tail vein injection into the isogeneic rats. The cultured and injected cells were both identified by immunocytochemistry and cultured cells were assayed using RT-PCR and Western blot.


RESULTS
Hepatectomized rat serum and HGF were demonstrated to have the effect of inducing transdifferentiation of ABMSCs into hepatocyte-like cells in vitro. The differentiated cells expressed albumin mRNA and albumin after 7 days++'s co-incubation. Albumin-expressing and CM-DiI positive hepatocyte-like cells were characterized in livers and spleens of the rats injected with the cultivated cells.


CONCLUSIONS
ABMSCs could transdifferentiate into hepatic parenchymal cells by hepatectomized rat serum or HGF.
Zhou
Bo-Jiang
BJ
Department of Anatomy, Histology and Embryology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Zhong
Cui-Ping
CP
Gu
Yun-di
YD
Zhang
Xin-Hua
XH
Liang
Chun-Min
CM
Wu
Chao-Qun
CQ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003470:Culture Media; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology*; D008115:Liver Regeneration; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D013234:Stem Cells / Q000166:cytology*
D003470:Culture Media; D017228:Hepatocyte Growth Factor