Different role of functional domains of hTR in DNA binding to telomere and telomerase reconstruction.
Even if template sequence of hTR played an essential role in telomere binding, a 326 nucleotide fragment of hTR containing template, pseudoknot, and CR4-5 domains is critical for both binding with telomeric DNA and reconstitution of telomerase activity. A functional study with antisense oligonucleotides suggested that targeted disruption of the template region efficiently abrogated both telomeric DNA binding and telomerase activity, whereas disruption of the CR4-5 region induced only loss of telomerase activity. hTR interacts with telomeric DNA via structural region composed of the template, pseudoknot, and CR4-5 domains, however, each structural domain plays a distinct role in telomere binding and telomerase activity reconstitution.
Yeo
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Cancer Metastasis Research Center, Yonsei Cancer Center, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
Rha
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Jeung
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Shen
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Yang
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An
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Roh
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Chung
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D001665:Binding Sites; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D012313:RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019098:Telomerase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*; D013698:Templates, Genetic
D016376:Oligonucleotides, Antisense; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; C422952:telomerase RNA; D012313:RNA; D004247:DNA; D019098:Telomerase