Bone marrow stem cells and biological scaffold for bone repair in aging and disease.
The loss of bone mass observed in aging enhances the risk of fractures. The process of bone repair in aging is slow and limited due to reduced activity of the osteoblasts. Bone marrow stem cells (MSCs) residing in the bone marrow are the progenitors for osteoblasts. The ability to enhance healing of bone defect in aging by MSCs can contribute in the prevention of the complications resulting from long-term immobilization that are especially fatal in old age. Our aim was to test the ability of MSCs inserted into a biological scaffold to enhance bone defect repair. Osteoprogenitor cells were selected from rat bone marrow stem cells cultured in DMEM medium supplemented with FCS, antibiotics, ascorbic acid, beta-glycerophosphate, and dexamethasone. The selected osteogenic subpopulation was identified by osteocalcin immunohistochemistry as well as Alizarin red S and von Kossa staining which are specific for bone matrix and mineral deposition. Committed osteoprogenitor cells cultured on the hydrogel scaffold were transplanted into the area of a rat tibia segmental bone defect and examined after 6 weeks. Radiology images revealed that 6 weeks post-implantaion, calcified material was present in the site of the defect, indicating new bone formation. It is concluded that committed osteogenic MSCs contained in a biocompatible scaffold can provide a promising surgical tool for enhancement of bone defect healing that will minimize the complications of bone repair in aging and disease.
Srouji
S
S
Department of Oral and Maxillofacial Surgery, Carmel Medical Center, Haifa, Israel.
Livne
E
E
D000375:Aging; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001798:Blood Proteins / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D017102:Fracture Healing; D005994:Glycerophosphates / Q000494:pharmacology; D006133:Growth Substances; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D014945:Wound Healing
D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D005994:Glycerophosphates; D006133:Growth Substances; D015675:Osteocalcin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003907:Dexamethasone; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid