Redox sensitive cysteine residues in calbindin D28k are structurally and functionally important.
Human calbindin D(28k) is a Ca(2+) binding protein that has been implicated in the protection of cells against apoptosis. In this study, the structural and functional significance of the five cysteine residues present in this protein have been investigated through a series of cysteine-to-serine mutations. The mutants were studied under relevant physiological redox potentials in which conformational changes were monitored using ANS binding. Urea-induced denaturations, as monitored by intrinsic tryptophan fluorescence, were also carried out to compare their relative stability. It was shown that the two N-terminal cysteine residues undergo a redox-driven structural change consistent with disulfide bond formation. The other cysteine residues are not by themselves sufficient at inducing structural change, but they accentuate the disulfide-dependent conformational change in a redox-dependent manner. Mass spectrometry data show that the three C-terminal cysteine residues can be modified by glutathione. Furthermore, under oxidizing conditions, the data display additional species consistent with the conversion of cysteine thiols to sulfenic acids and disulfides to disulfide-S-monoxides. The biological function of calbindin D(28k) appears to be tied to the redox state of the cysteine residues. The two N-terminal cysteine residues are required for activation of myo-inositol monophosphatase, and enzyme activation is enhanced under conditions in which these residues are oxidized. Last, oxidized calbindin D(28k) binds Ca(2+) with lower affinity than does the reduced protein.
Cedervall
Tommy
T
Department of Chemistry, Haverford College, 370 Lancaster Avenue, Haverford, Pennsylvania 19041, USA. tommy.cedervall@yahoo.com
Berggård
Tord
T
Borek
Valerie
V
Thulin
Eva
E
Linse
Sara
S
Akerfeldt
Karin S
KS
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D002021:Buffers; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000378:metabolism; D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D004789:Enzyme Activation / Q000235:genetics; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D011489:Protein Denaturation; D017510:Protein Folding; D064030:S100 Calcium Binding Protein G / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000737:chemistry; D014508:Urea / Q000737:chemistry
D000817:Anilino Naphthalenesulfonates; D002021:Buffers; C576498:CALB1 protein, human; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan; D014508:Urea; D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase; D005978:Glutathione; D003545:Cysteine; D002118:Calcium