Lateral gene transfer in the deep sea of Mariana Trench: identification of nar gene cluster encoding membrane-bound nitrate reductase from Pseudomonas sp. strain MT-1.
The genes encoding membrane-bound nitrate reductase and its locus from Pseudomonas sp. strain MT-1, which is isolated from the sediment of Mariana Trench, were identified. To some extent, the gene organization in the cluster was different from those of other Pseudomonads. Quite interestingly, two genes encoding putative nitrate transporter (narK and narM) showed higher homologies to counterparts of organisms belonging to other genera than those of Pseudomonads. Especially, narM showed no significant homology to the genes for nitrate transporter of Pseudomonads, and was homologous to those of some marine bacteria. Further, arrangements of NarL- and Fnr-binding motifs in the cluster were different from those of P. stutzeri, closely related strain with MT-1. These observations clearly indicated that lateral transfer of genes in nar gene cluster had occurred in deep sea, and it may contribute to bacterial adaptation to environment of there.
Tamegai
Hideyuki
H
Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajosui, Setagaya-ku, Tokyo, Japan. htamegai@chs.nihon-u.ac.jp
Kato
Chiaki
C
Horikoshi
Koki
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D040461:Gene Components; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D010140:Pacific Ocean; D010802:Phylogeny; D011549:Pseudomonas / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D009565:Nitrate Reductases; D050901:Nitrate Reductase