High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus.
A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was overproduced in larger amounts in the thermophile than in Escherichia coli. The protein purified from the thermophile was active in PCR.
Moreno
Renata
R
Centro de Biología Molecular Severo Ochoa CSIC-UAM, Campus de Cantoblanco, 28049 Madrid, Spain.
Haro
Amparo
A
Castellanos
Angel
A
Berenguer
José
J
D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005821:Genetic Techniques; D006639:Histidine; D016133:Polymerase Chain Reaction; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015854:Up-Regulation
D006639:Histidine; C068508:Tth polymerase; D004259:DNA-Directed DNA Polymerase