5-aminolevulinic acid mediated photodynamic therapy of Lewis lung carcinoma: a role of tumor infiltration with different cells of immune system.
OBJECTIVE
To investigate the effect of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) as a photosensitizer on the character of infiltration of experimental tumors by different cells of immune system.


METHODS
The effect of ALA-PDT on subcutaneously implanted Lewis lung carcinoma in c57Bl/6 mice was studied. ALA at a dose of 500 mg/kg was given per os, and 4 h later tumors were subjected to laser irradiation (632 nm, 150 mW/cm(2), 20 min). The evaluation of the tumor infiltration by myelomonocytic and lymphoid cells and oxygen-dependent metabolism of peritoneal macrophages (NBT-test) were carried out.


RESULTS
It is shown that ALA-PDT resulted in fast and massive infiltration of irradiated tumors by myelomonocytic cells, stimulation of the peritoneal macrophages metabolic activity and augmentation of the content of tumor infiltrating lymphocytes.


CONCLUSIONS
The immunomodulation after ALA-PDT occurs via generation of inflammation and direct laser light activation of immune system cells.
Skivka
Larisa M
LM
T.G. Shevchenko Kyiv National University, Kyiv, Ukraine. realmed@i.com.ua
Gorobets
Oksana B
OB
Kutsenok
Vadim V
VV
Lozinsky
Miron O
MO
Borisevich
Anatoliy N
AN
Fedorchuk
Alexander G
AG
Kholin
Vladimir V
VV
Gamaleya
Nikolai F
NF
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D007155:Immunologic Factors; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000502:physiology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*
D007155:Immunologic Factors; D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid