Optimization of chitosan film as a substitute for animal and human epidermal sheets for in vitro permeation of polar and non polar drugs.
The present investigation is aimed at preparing chitosan films capable of simulating the flux of modal drugs, 5-fluorouracil (5-FU) and indomethacin (INDO), across rat, rabbit and human cadaver epidermal sheets. Application of statistical design revealed that the concentration of chitosan, crosslinking time and concentration of crosslinking agent significantly influenced the in vitro flux of 5-FU and INDO across chitosan films. Multiple linear regression revealed a linear influence of all these active variables on 5-FU and INDO flux. It was deduced from atomic absorption spectroscopic analyses, DSC and IR spectroscopic data that 5% (m/V) sodium tripolyphosphate (NaTPP) produced optimum crosslinking of chitosan films. The in vitro permeation of both 5-FU and INDO across optimized film formulations was found to be comparable to that obtained across rat, rabbit and human epidermal sheets. These results indicate that optimized chitosan films have a potential to be developed as a substitute for animal and human cadaver epidermal sheets for preliminary in vitro permeation studies.
Rana
Vikas
V
Department of Pharmaceutical Sciences, Government Polytechnic for Women, Patiala-147002, India.
Babita
Kumar
K
Goyal
Dinesh
D
Gorea
Rakesh
R
Tiwary
Ashok
A
D000279:Administration, Cutaneous; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D016503:Drug Delivery Systems; D004817:Epidermis / Q000737:chemistry; D005472:Fluorouracil / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D008954:Models, Biological; D010539:Permeability; D011122:Polyphosphates / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D018709:Statistics, Nonparametric
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011122:Polyphosphates; D048271:Chitosan; C005692:triphosphoric acid; D005472:Fluorouracil; D007213:Indomethacin