[Constructing the recombinant of pET28a-TAT-LacZ].
A synthesized double-stranded oligomeric nucleotide encoding 11-amino acid TAT protein transduction domain3 was inserted into pET28a vector after 6 histidine coding sequence, LacZ gene from pcDNA4/Myc-His/LacZ was digested by EcoR I and Hind III, then cloned into pET28a-TAT. The highly expressed TAT-beta-galactosidase was purified by affinity chromatography. TAT-beta-Gal fusion protein can across vascular smooth muscle cells in vitro. This result open new possibility for direct delivery of protein into tissues for therapy.
Yan
Shi-Rong
SR
Department of Clinical laboratory, Shiyan Peoples Hospital of Yunyang medical college, Shiyan, Hubei 442000, China. graceyan@netease.com
Gong
Jian
J
Yan
Jie
J
Qiu
Yun-Cheng
YC