title: Isolation and molecular characterisation of equine rotaviruses from Germany.
abstract: A total of 26 rotavirus positive faecal samples of diarrhoeal foals, and 8 equine rotavirus isolates were examined. Viral RNA patterns were generated, G typing was performed by PCR, and a P[12]-specific DNA probe was developed for P typing. Furthermore, five equine rotavirus isolates were sequenced in the genomic regions coding for VP7 and part of VP4. Rotaviruses of genotype G3 P[12] were found in 22 faecal samples and G14 P[12] type could be found in 4 faecal samples. These findings confirm that in Germany G3 P[12] is the predominating type of equine rotaviruses.
authors.affiliation: Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, D-07743 Jena, Germany. m.elschner@jena.bfav.de
authors.forename: Mandy
authors.initials: M
authors.lastname: Elschner
authors.forename: Christina
authors.initials: C
authors.lastname: Schrader
authors.forename: Helmut
authors.initials: H
authors.lastname: Hotzel
authors.forename: Jutta
authors.initials: J
authors.lastname: Prudlo
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.forename: Werner
authors.initials: W
authors.lastname: Eichhorn
authors.forename: Werner
authors.initials: W
authors.lastname: Herbst
authors.forename: Peter
authors.initials: P
authors.lastname: Otto
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000662:veterinary; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D005838:Genotype; D005858:Germany; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins