title: SigB-dependent in vitro transcription of prfA and some newly identified genes of Listeria monocytogenes whose expression is affected by PrfA in vivo.
abstract: Recent studies have identified several new genes in Listeria monocytogenes which are positively or negatively affected by PrfA and grouped into three classes (E. Milohanic et al., Mol. Microbiol. 47:1613-1625, 2003). In vitro transcription performed with promoters of some class III genes showed strict SigB-dependent but PrfA-independent transcription initiation. Transcription starting at the prfA promoter PprfA2 was also optimal with SigB-loaded RNA polymerase, suggesting a direct link between SigB- and PrfA-dependent gene expression.
authors.affiliation: Biocenter (Microbiology), University of Würzburg, 97074 Würzburg, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Rauch
authors.forename: Qin
authors.initials: Q
authors.lastname: Luo
authors.forename: Stefanie
authors.initials: S
authors.lastname: Müller-Altrock
authors.forename: Werner
authors.initials: W
authors.lastname: Goebel
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000502:physiology; D010454:Peptide Termination Factors; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D010454:Peptide Termination Factors; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D015534:Trans-Activators