5-hydroxyuracil can form stable base pairs with all four bases in a DNA duplex.
NMR studies, UV-monitored melting experiments, and ab initio calculations show that 5-hydroxyuracil, produced by the oxidative de-amination of cytosines by reactive oxygen species, can form stable base-pairs with dA, dG, dC and dT residues in a DNA duplex, providing a basis for the in-vivo incorporation of 5-hydroxyuracil during DNA replication.
Thiviyanathan
Varatharasa
V
Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Somasunderam
Anoma
A
Volk
David E
DE
Gorenstein
David G
DG
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry*; D010084:Oxidation-Reduction; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry
D009705:Nucleosides; C045975:5-hydroxyuracil; D014498:Uracil; D004247:DNA