Characterization of allergen-specific monocyte-derived dendritic cells generated from monocytes by a single-step procedure: effect on naïve and memory T cells.
BACKGROUND
Dendritic cells are one of the most potent antigen-presenting cells and when pulsed with allergen can modulate allergen-specific T-cell responses. We sought to establish a single-step method by which to generate allergen-specific monocyte-derived dendritic cells (MoDCs).


METHODS
Dermatophagoides farinae (Df)-prepulsed MoDCs were generated from monocytes by culturing with Df in the presence interleukin (IL)-4, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumour necrosis factor (TNF)-alpha simultaneously. Df-prepulsed MoDC were incubated with autologous naive and memory T cells in the absence of recall antigen, then proliferation and cytokine production by T cells was determined.


RESULTS
Generation of allergen-prepulsed MoDCs was confirmed by examining expression of surface molecules. Df-prepulsed MoDC selectively induced proliferation of Df-specific T cells in the absence of recall antigen. Under these conditions, Df-prepulsed MoDCs augmented but did not alter the cytokine production profile. In addition, Df-prepulsed MoDCs activated naive T cells leading to proliferation and selective production of IFN-gamma in allergic patients but not in healthy subjects.


CONCLUSIONS
These results suggest that Df-prepulsed MoDC generated from monocytes by a simple single-step manipulation can induce Df-specific cellular responses from both naive and memory T cells in the absence of recall antigen, and these cells potentially can be utilized as immune adjuvants in allergen-specific immunotherapy.
Takishita
T
T
Department of Otolaryngology - Head & Neck Surgery, Okayama University Graduate Schoolof Medicine and Dentistry, Okayama, Japan.
Okano
M
M
Takahashi
K
K
Yoshino
T
T
Sugata
Y
Y
Hattori
H
H
Ohuchi
S
S
Ogawa
T
T
Nishizaki
K
K
D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D000954:Antigens, Surface / Q000378:metabolism; D016022:Case-Control Studies; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis; D003584:Cytological Techniques; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D009000:Monocytes / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D039741:Antigens, Dermatophagoides; D000954:Antigens, Surface; D016207:Cytokines