[Expression of ferritin receptor on placental microvillous membrane and its role in maternal-fetal iron transport].
OBJECTIVE
In order to investigate the expression of ferritin receptor (FnR) on human placental microvillous membrane in mid-pregnancy and its implication.


METHODS
Radioligand binding assay was used, with horse spleen 125I-apo-ferritin as radioligand.


RESULTS
The FnR expression in normal placental microvillous membrane in mid-pregnancy was (9.63 +/- 4.72) x 10(12) sites/mg pro, similar to that in normal placental of term delivery,and the dissociation constants (Kd) between them had also no significant difference: Competitive binding assay indicated that the receptor bound basic ferritin with higher affinity than acidic one.


CONCLUSIONS
The expression of FnR in mid-pregnancy almost come to mature level; The FnR on placenta might be involved in maternal-fetal iron transport, transfering iron from mother to fetal.
Kong
P
P
Oncology, Second Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041.
Liao
Q
Q
Luo
Q
Q
Li
Z
Z
Fu
R
R
Yang
X
X
D001052:Apoferritins / Q000378:metabolism; D001667:Binding, Competitive; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D005865:Gestational Age; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins / Q000378:metabolism*; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D008871:Microvilli / Q000378:metabolism; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013379:Substrate Specificity
D033862:Iron-Binding Proteins; D011956:Receptors, Cell Surface; C027293:ferritin receptor; D001052:Apoferritins; D007501:Iron