Surface bio-magnetism on bacterial cells adhesion and surface proteins secretion.
Extensive research works have been done on using magnetic fields on biological organism, but the results till date have been controversial [D.O. Carpenter, S. Ayrapetyan (Eds.), Biological Effects of Electric and Magnetic Fields, vol. 1, Academic Press, San Diego, 1994]. In spite of this, the study of surface magnetic effects on bacterial adhesion and cell growth has not been rigorously explored. The effects of surface magnetism, using perpendicularly polarized magnetic media, are evaluated on Bacillus licheniformis, a widely used bacterium in brewery [L. Kandra, a-Amylases of medical and industrial importance. J. Mol. Struct. (Theochem.), in press] and pharmaceutical [H. Ikram-ul et al., Production of alph amylase by Bacillus licheniformis using an economical medium. Bioresour. Technol. 87 (2003) 57-61] industries, by observing its adhesion and growth behavior. At different spin directions, we are able to observe a change on the biofilm formation, protein synthesis, and cell growth rate. Given that surface energy can easily penetrate through cells, this approach is an advantage over existing techniques that require direct physical contact to target cells. It also presents a new technique to cell adhesion and synthesis of surface proteins.
Chua
Loh-You
LY
School of Mechanical and Production Engineering, Nanyang Technological University, Nanyang Ave., Singapore 639798, Singapore.
Yeo
Swee-Hock
SH
D018829:Adhesins, Bacterial / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism; D001422:Bacterial Adhesion / Q000528:radiation effects; D001426:Bacterial Proteins / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms; D002448:Cell Adhesion; D016248:Computer Storage Devices; D003470:Culture Media; D046508:Culture Techniques; D004574:Electromagnetic Fields; D008280:Magnetics; D008565:Membrane Proteins / Q000378:metabolism; D008853:Microscopy; D018625:Microscopy, Atomic Force; D013499:Surface Properties; D013997:Time Factors
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D001672:Biocompatible Materials; D003470:Culture Media; D008565:Membrane Proteins