Generation of a polyclonal rabbit anti-mouse tissue factor antibody by nucleic acid immunisation.
Tissue factor (TF) the cellular receptor and cofactor for factor VII, initiates coagulation and has also been implicated in several coagulation-independent functions, including inflammation, angiogenesis and tumour metastasis. Investigations of TF expression in mouse models of these processes has been limited by the availability of antibodies that specifically recognise mouse TF. We have generated a rabbit polyclonal antibody to mTF by DNA immunisation. This has yielded an antiserum that recognises native mTF in immunohistochemical and flow cytometric analyses. Furthermore, the antiserum is inhibitory in coagulation assays. This antiserum will be a valuable investigative tool in the analysis of mTF expression.
Mumford
Andrew D
AD
Haemostasis and Thrombosis, MRC Clinical Sciences Centre, The Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, United Kingdom.
Chen
Daxin
D
Dorling
Anthony
A
Kemball-Cook
Geoffrey
G
McVey
John H
JH
D000818:Animals; D000917:Antibody Formation; D003001:Cloning, Molecular; D004247:DNA / Q000008:administration & dosage* / Q000276:immunology; D007106:Immune Sera / Q000096:biosynthesis*; D007114:Immunization / Q000379:methods*; D007118:Immunoassay; D051379:Mice; D009000:Monocytes; D011817:Rabbits; D013925:Thromboplastin / Q000032:analysis / Q000235:genetics* / Q000276:immunology*
D007106:Immune Sera; D004247:DNA; D013925:Thromboplastin