Molecular cloning and characterization of a novel cold-regulated gene from Capsella bursa-pastoris.
A novel cor gene was cloned from Capsella bursa-pastoris (designated Cbcor15b) by RACE-PCR. The full-length cDNA of Cbcor15b was 652bp and contained a 417bp open reading frame (ORF) encoding a 139-amino acid hydrophilic protein. Multiple alignments showed that Cbcor15b had high similarity with other cold-regulated genes from Arabidopsis thaliana (cor15b, cor15a), Brassica napus (bn115, bn19 and bn26) and genes encoding late embryogenesis abundant (LEA) proteins. The predicted CbCOR15B protein was found to have a potential chloroplast signal sequence cleavage site, two cAMP- and cGMP-dependent protein kinase (PKA and PKG) phosphorylation sites. Cold acclimation assay showed that Cbcor15b was relevant to cold acclimation. Our study implies that Cbcor15b might have similar functions possessed by other cor genes in increasing plants' freezing tolerance.
Liu
Sixiu
S
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SITU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China.
Wang
Xinglong
X
Fan
Zhengqiu
Z
Pang
Yongzhen
Y
Sun
Xiaofen
X
Wang
Xiangrong
X
Tanga
Kexuan
K
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D029687:Capsella / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017385:Sequence Homology
D029681:Arabidopsis Proteins; C116166:COR15 protein, Arabidopsis; D018076:DNA, Complementary; D010940:Plant Proteins