title: CXCR3-positive B cells found at elevated frequency in the peripheral blood of patients with MALT lymphoma are attracted by MIG and belong to the lymphoma clone.
abstract: Chemokine receptors mediate the migration of lymphocytes through binding of their ligands. CXCR3 is expressed in Th1 T cells; however, CXCR3 was recently reported in B-cell mucosa-associated lymphoid tissue (MALT)-type lymphoma and splenic marginal zone lymphoma. To investigate whether CXCR3-positive B lymphocytes in peripheral blood (PB) migrate to MALT and spleen, and whether the lymphoma clone is present in PB, we studied 16 cases of MALT lymphoma. In MALT cases, CXCR3-positive B lymphocytes in PB could migrate to MIG, the CXCR3 ligand. Immunohistochemical analysis showed that MALT lymphoma cells expressed CXCR3, whereas epithelial glands and/or stromal cells expressed MIG. In the PCR analysis for VH gene rearrangements, MALT lymphoma showed monoclonal or oligoclonal bands. In addition, in 8 of 16 MALT cases, the VH gene rearrangement of MALT lymphoma had the same bands as the CXCR3-positive B lymphocytes in PB. In 4 cases, the same clones of DNA sequences were confirmed in MALT lymphoma and CXCR3-positive B lymphocytes of PB. The findings support the theory that CXCR3-positive B lymphocytes in PB of MALT patients belong to the lymphoma clone and migrate to MIG-expressing mucosa-associated lymphoid tissue. It seemed to be associated with the dissemination of MALT lymphoma.
authors.affiliation: Department of Pathology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suefuji
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: Kennosuke
authors.initials: K
authors.lastname: Karube
authors.forename: Riko
authors.initials: R
authors.lastname: Kawano
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nabeshima
authors.forename: Junnji
authors.initials: J
authors.lastname: Suzumiya
authors.forename: Naofumi
authors.initials: N
authors.lastname: Hayabuchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kikuchi
mesh_terms: D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000502:physiology*; D016022:Case-Control Studies; D002465:Cell Movement; D054370:Chemokine CXCL9; D019743:Chemokines, CXC / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008024:Ligands; D018442:Lymphoma, B-Cell, Marginal Zone / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane; D016133:Polymerase Chain Reaction; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000032:analysis*
chemical_list: C080810:CXCL9 protein, human; C515187:CXCR3 protein, human; D054370:Chemokine CXCL9; D019743:Chemokines, CXC; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine