[A non-radioactive method used for Reverse Northern].
In this paper, a simple, rapid and safe method of Reverse Northern was introduced. A non-radioactive labeling system was used to label the probe. During the process, the quality of probe was normalized three times to rule out the possibility of false results. Samples were dotted as microarray and were hybridized effectively. The method is effective and expected to be widely used in laboratories.
Zhang
Yu-Wei
YW
Department of Pathology, School of Basic Medical Sciences, Southeast University, Nanjing 210 009, China. zyw917@hotmail.com
Lai
Mao-De
MD
D000230:Adenocarcinoma / Q000235:genetics; D000236:Adenoma / Q000235:genetics; D015152:Blotting, Northern / Q000379:methods*; D003110:Colonic Neoplasms / Q000235:genetics; D015342:DNA Probes; D018076:DNA, Complementary / Q000032:analysis; D004076:Digoxigenin; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007413:Intestinal Mucosa / Q000737:chemistry; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015342:DNA Probes; D018076:DNA, Complementary; D012334:RNA, Neoplasm; D004076:Digoxigenin