Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential of a large fraction of B-cell specific and housekeeping genes whose function can be analyzed by disruption in the chicken DT40 B-cell line.
Caldwell
Randolph B
RB
Institute of Molecular Radiobiology, GSF, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany. randolph.caldwell@gsf.de <randolph.caldwell@gsf.de>
Kierzek
Andrzej M
AM
Arakawa
Hiroshi
H
Bezzubov
Yuri
Y
Zaim
Jolanta
J
Fiedler
Petra
P
Kutter
Stefan
S
Blagodatski
Artem
A
Kostovska
Diyana
D
Koter
Marek
M
Plachy
Jiri
J
Carninci
Piero
P
Hayashizaki
Yoshihide
Y
Buerstedde
Jean-Marie
JM
D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001482:Base Composition / Q000235:genetics; D002060:Bursa of Fabricius / Q000166:cytology* / Q000378:metabolism; D002460:Cell Line; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D019295:Computational Biology; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics*; D030541:Databases, Genetic; D008214:Lymphocytes / Q000378:metabolism*; D009928:Organ Specificity; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014158:Transcription, Genetic / Q000235:genetics
D030161:Avian Proteins; D018387:Codon, Initiator; D018076:DNA, Complementary; D012333:RNA, Messenger