Anti-clastogenic ingredients in Cassia nomame extract.
The suppressing effect of the hot-water extract of Cassia nomame (Sieb.) HONDA was studied on the frequency of chromosomal aberrations in Chinese hamster ovary K1 (CHO-K1) cells. CHO-K1 cells were pretreated with 2.5 microM Mitomycin C (MMC) for 1 h and incubated with or without the extract in medium for 10-24 h. The frequency of chromosome aberrations in observed 100 metaphase cells was significantly lower with the extract than that without the extract. Moreover, the suppressing effect of the four fractions collected by high performance liquid chromatography (HPLC) was also examined on the same procedure. The frequency of cells with chromosome aberrations in cells cultured with each collected fraction was lower than in those without the extract. The suppressing effect of the collected fractions on chromosomal aberrations, however, was less than that of the total extract. This result suggests that the ingredients which have the suppressing effect of chromosomal aberrations are also contained in the other fraction of the extract.
Konishi
Taiji
T
Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan. konisi@stn.naagaokaut.ac.jp
Naitou
Kazufumi
K
Kadowaki
Shin'ichi
S
Takahara
Yoshinori
Y
Yamamoto
Koji
K
D000818:Animals; D016587:Antimutagenic Agents / Q000032:analysis* / Q000494:pharmacology; D016466:CHO Cells; D002366:Cassia / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D008940:Mitotic Index; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
D016587:Antimutagenic Agents; D010936:Plant Extracts