title: Long-term treatment effects of Pueraria mirifica phytoestrogens on parathyroid hormone and calcium levels in aged menopausal cynomolgus monkeys.
abstract: To determine the effect of Pueraria mirifica (PM) on serum parathyroid hormone (PTH) and calcium levels on aged menopausal monkeys (Macaca fascicularis), subjects were treated with 10, 100, or 1,000 mg/day of PM. Blood samples were collected every 5 days for 30, 90, and 60 days during pre-treatment, treatment, and post-treatment periods, respectively. Sera were assayed for PTH, estradiol, and calcium levels. PM-1,000 had the strongest effect on the decrease in PTH (0.001<P< or =0.05) and calcium levels (0.001<P< or =0.03) during the treatment period. PTH levels remained low for the first 15 days of the post-treatment period (0.01< or =P < or =0.05). PM-10 induced a significant decrease in PTH level on day 80 (P=0.02) during the treatment period and a significant decrease in calcium level on day 75 (P<0.01). There were no changes in serum PTH and calcium levels throughout the study period in the PM-100 group. Estradiol levels decreased significantly during the treatment period in all treatment groups. The results suggest that long-term treatment with 1,000 mg/day of PM decreases serum PTH and calcium levels in aged menopausal monkeys, indicating that PM ameliorates bone loss caused by estrogen deficiency.
authors.affiliation: Biological Science Ph.D. Program, Faculty of Science, Chulalongkorn University, Bangkok, Tokyo, Japan.
authors.forename: Hataitip
authors.initials: H
authors.lastname: Trisomboon
authors.forename: Suchinda
authors.initials: S
authors.lastname: Malaivijitnond
authors.forename: Juri
authors.initials: J
authors.lastname: Suzuki
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Hamada
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000097:blood; D005260:Female; D008252:Macaca fascicularis; D008593:Menopause; D010281:Parathyroid Hormone / Q000097:blood / Q000378:metabolism*; D048789:Phytoestrogens / Q000378:metabolism* / Q000627:therapeutic use*; D028321:Plant Preparations / Q000627:therapeutic use; D029908:Pueraria / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010281:Parathyroid Hormone; D048789:Phytoestrogens; D028321:Plant Preparations; D004958:Estradiol; D002118:Calcium