[Determination of baicalin in Xiaoyanling Decoction by high-performance liquid chromatography].
OBJECTIVE
To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction.


METHODS
HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H(2)O:H(3)PO(4)=53:0.2) 40:60, and the detective wavelength was 277 nm.


RESULTS
The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%).


CONCLUSIONS
The content of baicalin in Xiaoyanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.
Jian
Jun-Yi
JY
School of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China. jiang14031@sina.com
Li
Xiang
X
Qiu
Rong-Li
RL
Zhang
Hai-Xia
HX
Wu
Zhi-Ping
ZP
D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D006801:Humans
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C038044:baicalin