Alternative promotion of the mouse acyl-CoA synthetase 6 (mAcsl6) gene mediates the expression of multiple transcripts with 5'-end heterogeneity: genetic organization of mAcsl6 variants.
We report four variants and alternative promoter usage for the mouse acyl-CoA synthetase 6 (mAcsl6) gene. The variants, which were organized into 26 exons and 25 introns spanning 55 kb of DNA on mouse chromosome 11, were classified according to their 5'-UTRs and alternative splicing of exon 13. Alignment of the nucleotide sequences showed that the mAcsl6 variant 1 (mAcsl6_v1) and mAcsl6_v2 used a different promoter and had different splicing patterns than mAcsl6_v3 and mAcsl6_v4. The results of the promoter analysis suggest that the mAcsl6 promoter 1 (mAcsl6_pr1) region has a negative regulatory function. To verify this result, we constructed id vector constructs that contained the promoter regions mAcsl6_pr1 and 2, and the chimeric transcript. Although the mAcsl6_pr1 region was deleted, the mAcsl6_v1 and 2 transcripts were detected consistently.
Lee
Eun Ju
EJ
Embryology and Gamete Biotechnology, School of Agricultural Biotechnology, Seoul National University, San 56-1, Shilim-Dong, Kwanak-Gu, Seoul 151-742, Republic of Korea.
Kim
Hi Chul
HC
Cho
Yong Yeon
YY
Byun
Sung June
SJ
Lim
Jeong Mook
JM
Ryoo
Zae Young
ZY
D020121:5' Untranslated Regions / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005091:Exons / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D014644:Genetic Variation / Q000235:genetics*; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016415:Sequence Alignment; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000235:genetics*
D020121:5' Untranslated Regions; D003066:Coenzyme A Ligases; C475689:Acsl6 protein, mouse