title: Tissue retention and adverse reaction after intravenous injection of hematoporphyrin derivatives in dogs.
abstract: We evaluated changes in hematology and chemical profile, and the tissue retention of hematoporphyrin derivative (HpD) following the intravenous injection in dogs. HpD at concentrations of 1, 5, 10, and 15 mg/kg was intravenously injected to 16 dogs (n=4 each) and complete blood count (CBC) and blood chemistry were performed on days 1, 3, 5, and 7 after the injection. To examine tissue retention, HpD (5 mg/kg) was administered to 15 dogs and 3 dogs were euthanized on days 1, 2, 7, 14, and 28 after the injection, respectively, to collect the skin, muscle, small intestine, spleen, kidney and liver as tissue samples. There were no significant changes in CBC and blood chemical profile except for an increase in LDH concentrations in dogs given 10 and 15 mg/kg of HpD at day 3. The levels of HpD retention in the tissues were ranked in the following order: liver > kidney > spleen > intestine > muscle > skin.
authors.affiliation: Department of Veterinary Surgery, Faculty of Agriculture, Tottori University, 101 Minami 4-chome, Koyama-cho, Tottori 680-8553, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yonemura
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Miyatake
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Toru
authors.initials: T
authors.lastname: Hirano
authors.forename: Isao
authors.initials: I
authors.lastname: Sakata
authors.forename: Saburo
authors.initials: S
authors.lastname: Minami
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D004285:Dogs / Q000378:metabolism*; D006415:Hematoporphyrins / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism*; D007275:Injections, Intravenous; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013997:Time Factors
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents; D007770:L-Lactate Dehydrogenase