[Analysis of codon usage in potato and its application in the modification of t-PA gene].
Bioperl-1.0 was used under Hongqi LINUX system to program the codon analysis software. According to the analysis of 98 codon DNA sequences with this software, the codon usage in potato was calculated and 4 codons have been inferred to the optimal codons. The codons of tissue plasminogen activator (t-PA) gene sequence have been reconstructed according to the results. The t-PA gene sequence containing the optimal codons of potato will be used for t-PA production by potato bioreactor.
Bai
Xi
X
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
Xu
Jian-Zhen
JZ
Li
Lin
L
Guo
Zheng
Z
Li
Jie
J
Zhu
Yan-Ming
YM
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D005815:Genetic Code; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012984:Software; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000235:genetics* / Q000378:metabolism
D003062:Codon; D010940:Plant Proteins; D010959:Tissue Plasminogen Activator