title: [9-cis retinoic acid induces apoptosis in HL-60 cells].
abstract: OBJECTIVE
  To investigate the apoptosis of HL-60 cells induced by 9-cis retinoic acid (9-cis RA), and illustrate the possible molecular mechanism.
  
  
  METHODS
  Apoptosis was detected by morphological observation, DNA electrophoresis and flow cytometric cell cycle analysis. Bcl-2 expression was detected by flow cytometry.
  
  
  RESULTS
  9-cis RA initiated apoptosis of HL-60 cells after inducing them irreversibly commited to differentiation. Bcl-2 was downregulated during the differentiation and apoptosis process. 9-cis RA was more potent than all-trans retinoic acid (ATRA) did in inducing terminal differentiation associated apoptosis and in downregulation of Bcl-2 expression.
  
  
  CONCLUSIONS
  9-cis RA can induce apoptosis in HL-60 cells. Downregulation of Bcl-2 expression appears to play an important role in the apoptosis of the differentiated leukemic cells.
authors.affiliation: Department of Hematology, Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing.
authors.forename: Z
authors.initials: Z
authors.lastname: Hui
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.forename: E
authors.initials: E
authors.lastname: Ai
authors.forename: R
authors.initials: R
authors.lastname: Zhao
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Ju
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Gao
mesh_terms: D000077556:Alitretinoin; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D000077556:Alitretinoin; D014212:Tretinoin