Design, synthesis, and application of particle-based fluorescence resonance energy transfer sensors for carbohydrates and glycoproteins.
This paper describes the development of novel particle-based fluorescence resonance energy transfer (FRET) sensors to quantify the concentration and monitor the binding affinity of carbohydrates and glycoproteins to lectins, which are carbohydrate binding proteins. The sensing approach is based on FRET between fluorescein (donor)-labeled lectin molecules, adsorbed on the surface of micrometric polymeric beads, and polymeric dextran molecules labeled with Texas Red (acceptor). The FRET efficiency of the donor-acceptor pair decreases in the presence of carbohydrates or glycoproteins that compete with the Texas Red-labeled dextran molecules on the lectinic binding sites. The inhibitory effect is concentration and time dependent. The sensing technique enables the discrimination between carbohydrates and glycoproteins based on their binding affinity to the FRET sensing particles as well as quantitative analysis of carbohydrates and glycoproteins in aqueous samples. In the future, the newly developed sensors could enable screening glycoprotein-based drugs for their binding affinity toward selective receptors.
Blagoi
Gabriela
G
Department of Chemistry, University of New Orleans, New Orleans, Louisiana 70148, USA.
Rosenzweig
Nitsa
N
Rosenzweig
Zeev
Z
D015374:Biosensing Techniques / Q000295:instrumentation*; D002241:Carbohydrates / Q000032:analysis*; D003208:Concanavalin A; D003911:Dextrans; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins / Q000378:metabolism; D011137:Polystyrenes; D013050:Spectrometry, Fluorescence; D014966:Xanthenes
D002241:Carbohydrates; D003911:Dextrans; D005456:Fluorescent Dyes; D006023:Glycoproteins; D037102:Lectins; D011137:Polystyrenes; D014966:Xanthenes; C042784:fluorescein isothiocyanate-concanavalin A; D003208:Concanavalin A; C034657:Texas red; D016650:Fluorescein-5-isothiocyanate