title: Oligomeric compounds formed from 2,5-xylidine (2,5-dimethylaniline) are potent enhancers of laccase production in Trametes versicolor ATCC 32745.
abstract: Numerous chemicals, including the xenobiotic 2,5-xylidine, are known to induce laccase production in fungi. The present study was conducted to determine whether the metabolites formed from 2,5-xylidine by fungi could enhance laccase activity. We used purified laccases to transform the chemical and then we separated the metabolites, identified their chemical structure and assayed their effect on enzyme activity in liquid cultures of Trametes. versicolor. We identified 13 oligomers formed from 2,5-xylidine. (4E)-4-(2,5-dimethylphenylimino)-2,5-dimethylcyclohexa-2,5-dienone at 1.25 x 10(-5) M was an efficient inducer, resulting in a nine-fold increase of laccase activity after 3 days of culture. Easily synthesized in one step (67% yield), this compound could be used in fungal bioreactors to obtain a great amount of laccases for biochemical or biotechnological purposes, with a low amount of inducer.
authors.affiliation: Unité de Phytopharmacie et Médiateurs Chimiques, INRA, Route de Saint-Cyr, 78026 Versailles Cedex, France.
authors.forename: Albert
authors.initials: A
authors.lastname: Kollmann
authors.forename: François-Didier
authors.initials: FD
authors.lastname: Boyer
authors.forename: Paul-Henri
authors.initials: PH
authors.lastname: Ducrot
authors.forename: Lucien
authors.initials: L
authors.lastname: Kerhoas
authors.forename: Claude
authors.initials: C
authors.lastname: Jolivalt
authors.forename: Isabelle
authors.initials: I
authors.lastname: Touton
authors.forename: Jacques
authors.initials: J
authors.lastname: Einhorn
authors.forename: Christian
authors.initials: C
authors.lastname: Mougin
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D004790:Enzyme Induction; D042845:Laccase / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D000814:Aniline Compounds; C016835:2,5-xylidene; D042845:Laccase