[Preparation and characterization of nanoemulsion].
OBJECTIVE
To prepare nanoemulsion-encapsulated BSA-FITC (NEBSA-FITC), study its characteristics, and measure its uptake by dendritic cells (DCs) and peritoneal macrophages.


METHODS
NEBSA-FITC was prepared by a method of interfacial polymerization.The encapsulation rate, drug-carrying capacity and stability of the nanoemulsion were determined by Sephadex-G100 chromatography. The shape and size of NEBSA-FITC were observed under electron microscope. The uptake of NEBSA-FITC by DCs and macrophage cells was detected by FACS and laser confocal microscopy.


RESULTS
The mean size of NEBSA-FITC was (25+/-10) nm. The encapsulation rate was 91%, the drug-carrying capacity was 0.091 g/L and NEBSA-FITC had a good stability. The FACS analysis showed that DCs and macrophage cells could take in more NEBSA-FITC than free BSA. The observation under laser confocal microscope found that NEBSA-FITC was located in the cytoplasm of DCs.


CONCLUSIONS
Nanoemulsion can be efficiently taken by DCs and macrophage cells, and therefore may be promising efficient carrier of APCs-targeted antitumor vaccine.
Sun
Yu-Jing
YJ
Department of Pathology, Fourth Military Medical University, Xi'an 710032, China. sunyuj@fmmu.edu.cn
Wu
Dao-Cheng
DC
Cao
Yun-Xin
YX
Sui
Yan-Fang
YF
D000818:Animals; D001692:Biological Transport; D002417:Cattle; D003713:Dendritic Cells / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism*; D004655:Emulsions; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D036103:Nanotechnology; D012710:Serum Albumin, Bovine / Q000378:metabolism
D004337:Drug Carriers; D004655:Emulsions; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate