title: Bovine herpesvirus 1 VP22 enhances the efficacy of a DNA vaccine in cattle.
abstract: For this study, the intercellular trafficking ability of bovine herpesvirus 1 (BHV-1) VP22 was applied to improve the efficacy of a DNA vaccine in calves. A plasmid encoding a truncated version of glycoprotein D (tgD) fused to VP22 was constructed. The plasmid encoding tgD-VP22 elicited significantly enhanced and more balanced immune responses than those induced by a plasmid encoding tgD. Furthermore, protection against a BHV-1 challenge was obtained in calves immunized with the plasmid encoding tgD-VP22, as shown by significant reductions in viral excretion. However, less significant protection was observed for animals vaccinated with the tgD-expressing plasmid, correlating with the lower level of immunity observed prechallenge. This is the first report of the use of VP22 as a transport molecule in the context of a DNA vaccine for a large animal species.
authors.affiliation: Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, SK S7N 5E3, Canada.
authors.forename: Chunfu
authors.initials: C
authors.lastname: Zheng
authors.forename: Lorne A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: Sylvia
authors.initials: S
authors.lastname: van Drunen Littel-van den Hurk
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D019556:COS Cells; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000276:immunology / Q000378:metabolism; D022283:Herpesvirus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D022283:Herpesvirus Vaccines; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014764:Viral Proteins; D015678:Viral Structural Proteins; C046998:bovine herpesvirus type-1 glycoproteins; C434023:tegument protein VP22, bovine herpesvirus 1