Molecular cloning and expression of two closely related GTP-binding proteins from zebrafish.
Developmentally regulated GTP-binding proteins (DRGs) are a subclass of GTP-binding proteins that have been discovered recently. Here we report two zebrafish DRG cDNA clones closely related to human and mouse DRG genes. The two DRG sequences showed a high degree of similarity (55% identity, 72% similarity) at the amino acids level. Whole mount in situ hybridization revealed expression of zebrafish DRGs maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic. The expression of these two genes in different tissues follows a similar pattern, suggesting that they may serve a similar function.
Wei
Dongwang
D
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Yao
Jihua
J
Yang
Xueyan
X
Cheng
Lu
L
Lu
Daru
D
Xue
Jinglun
J
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010063:Ovum / Q000378:metabolism; D010802:Phylogeny; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; C409877:DRG2 protein, human; C078418:developmentally regulated GTP-binding protein; D019204:GTP-Binding Proteins