[Isolation and characterization of SIR73 gene fragment in salt resistant mutant of wheat involved in salt stress].
cDNA-AFLP (amplified fragment length polymorphism) is used to isolate genes differentially expressed in two wheat lines with the different resistance to NaCl derived from a single seed. A lot of cDNA fragments related to salt tolerance are obtained. Of with the number 73 cDNA fragment encodes for a transcription factors with an 32% similarity to human transcription factors in the relative amino acid which is named SIR73. Northern analysis confirms that SIR73 is strongly induced by NaCl stress and the expression in SR is more strongly induced than in SS.SIR73 may be involved in the regulation of gene expression in salt stress in wheat.
Chen
Gui-Ping
GP
College of Life Science, Hebei Normal University, Shijiazhuang,050016, China.
Ma
Wen-Shi
WS
Huang
Zhan-Jing
ZJ
Shen
Yin-Zhu
YZ