Evidence for preferential genotyping of a minority human immunodeficiency virus population due to primer-template mismatching during PCR-based amplification.
Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.
Martinez-Picado
Javier
J
IrsiCaixa Foundation, Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain. javiermp@ns.hugtip.scs.es
Morales-Lopetegi
Kristina
K
Villena
Cristina
C
Gutiérrez
Carolina
C
Izquierdo
Nuria
N
Marfil
Silvia
S
Clotet
Bonaventura
B
Ruiz
Lidia
L
D000328:Adult; D001482:Base Composition; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D016333:HIV Protease / Q000235:genetics*; D017320:HIV Protease Inhibitors / Q000494:pharmacology; D015497:HIV-1 / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic
D017931:DNA Primers; D004279:DNA, Viral; D017320:HIV Protease Inhibitors; D016333:HIV Protease