Evaluation of nested PCR in diagnosis of typhoid fever.
In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.
Prakash
Pradyot
P
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. pradyot_micro@yahoo.co.in
Mishra
Om Prakash
OP
Singh
Alok Kumar
AK
Gulati
Anil Kumar
AK
Nath
Gopal
G
D000372:Agglutination Tests; D001769:Blood / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction / Q000379:methods*; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014435:Typhoid Fever / Q000175:diagnosis*
D003470:Culture Media