Simultaneous determination of aflatoxins and ochratoxin A in food using a fully automated immunoaffinity column clean-up and liquid chromatography-fluorescence detection.
An automated HPLC method for the simultaneous detection of aflatoxins (AF) and ochratoxin A (OA) has been developed. The method uses an immunoaffinity column containing antibodies specific to both AF and OA. The samples were extracted with an acetonitrile/water mixture and diluted with phosphate buffer saline (PBS). The aqueous extracts were then transferred to an ASPEC HPLC system for automated clean-up using AflaOchra immunoaffinity columns. OA and AF were quantified using HPLC with fluorescence detection, with a run time of approximately 40 min. Limits of quantification were estimated as 0.2 microg/kg for OA and AFB1, AFB2, AFG1 and AFG2. Initial validation of this method gave average recoveries of 72-101% for OA and AF for a range of food products (maize cereal products and peanut butter). Within laboratory RSDr and RSDR for a 5.0 microg/kg spike level in maize cereals was found to be 7.6-10.1% (AF and OA) and 10.2-13.8%, respectively.
Chan
D
D
Central Science Laboratory, Sand Hutton, York, UK. d.chan@csl.gov.uk
MacDonald
S J
SJ
Boughtflower
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V
Brereton
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P
D000348:Aflatoxins / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000348:Aflatoxins; D009793:Ochratoxins; C025589:ochratoxin A