title: Time course of discordant BMIPP and thallium uptake after ischemia and reperfusion in a rat model.
abstract: Serial changes in fatty acid metabolism or use associated with acute ischemia and reperfusion were examined in rat hearts.
  
  
  METHODS
  Male Wistar rats were subjected to occlusion of the left coronary artery for 20 min followed by reperfusion. After release of the occlusion, groups of animals were allowed to recover for intervals of 20 min (n = 9), 1 d (n = 9), 3 d (n = 6), 7 d (n = 6), or 30 d (n = 6). Hearts were excised 15-20 min after injection of 0.74 MBq of (125)I-15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid (BMIPP) and 14.8 MBq of (201)Tl. One minute before resection, the left coronary artery was reoccluded and 185 MBq of (99m)Tc-sestamibi were injected to document the area at risk. Triple-tracer autoradiography was performed to assess tracer uptake. Uptake ratios of BMIPP and (201)Tl in the area at risk were calculated on the basis of the count density in the lesion divided by that in the normally perfused area.
  
  
  RESULTS
  (201)Tl uptake did not change throughout the observation period (P = 0.25). In contrast, BMIPP uptake increased early in the acute phase (20 min and 1 d), decreased during the subacute phase (7 d), and subsequently recovered in the chronic phase (30 d).
  
  
  CONCLUSIONS
  The present investigation clearly illustrated that BMIPP uptake is higher than (201)Tl uptake in the acute phase, that BMIPP uptake is lower than (201)Tl uptake in the subacute phase, and that BMIPP uptake and (201)Tl uptake are similar in the chronic phase. These results yield data essential to the precise interpretation of BMIPP images.
authors.affiliation: PET Center, Kanazawa Cardiovascular Hospital, Kanazawa, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Higuchi
authors.forename: Junichi
authors.initials: J
authors.lastname: Taki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nakajima
authors.forename: Seigo
authors.initials: S
authors.lastname: Kinuya
authors.forename: Masanobu
authors.initials: M
authors.lastname: Namura
authors.forename: Norihisa
authors.initials: N
authors.lastname: Tonami
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000493:pharmacokinetics*; D006321:Heart / Q000000981:diagnostic imaging*; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D007462:Iodobenzenes / Q000493:pharmacokinetics*; D008297:Male; D008657:Metabolic Clearance Rate; D009206:Myocardium / Q000378:metabolism*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000000981:diagnostic imaging* / Q000378:metabolism*; D013793:Thallium / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D005227:Fatty Acids; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D019275:Radiopharmaceuticals; C049338:iodofiltic acid; C027549:thallium chloride; D013793:Thallium