Two-dimensional electrophoresis protein profiling as an analytical tool for human acute leukemia classification.
Two-dimensional electrophoresis (2-DE) was used to profile the proteins of leukemic cells from 61 cases of akute leukemia (AL) characterized by the French-American-British (FAB) classification. The differentially expressed protein spots were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electrospray ionization-tandem MS (ESI-MS/MS). The distinct protein profiles (DPPs) of AL FAB subtypes were explored successfully, including acute myeloid leukemia (AML), its subtypes (M2, M3, and M5), and acute lymphoid leukemia (ALL), which were homogeneous within different samples of the same subgroup but clearly differed from all other subgroups. We also found a group of proteins differentially expressed between AL cells and normal white blood cells. Among the DPPs of AL subtypes, some proteins have been reported, but most of them were first reported here to mark AML differentiation and to discriminate AML from ALL. These data show that 2-DE protein profiling could be used as an analytical tool for facilitating molecular definition of human AL classification and understanding the mechanism of leukemogensis, and the extension of the present analysis to the currently less well-defined AL will identify additional subgroups and may promote the identification of new targets for specific treatment approaches.
Cui
Jiu-Wei
JW
Department of Hematology and Oncology, The First Clinical Hospital of Jilin University, Changchun, China.
Wang
Jie
J
He
Kun
K
Jin
Bao-Feng
BF
Wang
Hong-Xia
HX
Li
Wei
W
Kang
Li-Hua
LH
Hu
Mei-Ru
MR
Li
Hui-Yan
HY
Yu
Ming
M
Shen
Bei-Fen
BF
Wang
Guan-Jun
GJ
Zhang
Xue-Min
XM
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007938:Leukemia / Q000145:classification*; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis*; D010449:Peptide Mapping
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins