title: Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria).
abstract: The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximately ethynylestradiol (EC50: 13-80 nM)>estrone (EC50: 218-241 nM)>DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 microM did not have any effect on in vitro vitellogenesis.
authors.affiliation: Institute for Risk Assessment Sciences (IRAS), Utrecht University, PO Box 80176, 3508 TD Utrecht, The Netherlands. t.rouhani@iras.uu.nl
authors.forename: T
authors.initials: T
authors.lastname: Rouhani Rankouhi
authors.forename: J T
authors.initials: JT
authors.lastname: Sanderson
authors.forename: I
authors.initials: I
authors.lastname: van Holsteijn
authors.forename: P
authors.initials: P
authors.lastname: van Kooten
authors.forename: A T C
authors.initials: AT
authors.lastname: Bosveld
authors.forename: M
authors.initials: M
authors.lastname: van den Berg
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D004970:Estrone / Q000494:pharmacology; D004997:Ethinyl Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008731:Methoxychlor / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D011896:Rana temporaria / Q000378:metabolism*; D014818:Vitellogenesis / Q000187:drug effects*; D014819:Vitellogenins / Q000096:biosynthesis / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol; D004958:Estradiol; D004967:Estrogens; D004970:Estrone; D004997:Ethinyl Estradiol; D008731:Methoxychlor; D010636:Phenols; D014819:Vitellogenins; C025810:ethinylestradiol-3-sulfate; C006780:bisphenol A; D000098826:Bisphenol A Compounds