title: Incubation of bovine PMNs with conditioned medium from BHV-1 infected peripheral blood mononuclear cells increases their susceptibility to Mannheimia haemolytica leukotoxin.
abstract: Active infection with bovine herpesvirus-1 (BHV-1) increases the susceptibility of cattle to secondary bacterial pneumonia with Mannheimia (Pasteurella) haemolytica A1. In the present study we found that bovine PMNs incubated with conditioned media from BHV-1 infected peripheral blood mononuclear cells (PBMCs) exhibited increased LFA-1 expression, enhanced LKT binding and increased LKT cytotoxicity. These effects were abrogated when the conditioned medium was pre-incubated with an anti-IL-1beta Mab before being added to the PMNs. These findings suggest that BHV-1 infection, and the resulting release of IL-1beta and perhaps other inflammatory cytokines, can stimulate activation of LFA-1 in bystander bovine PMNs, thus enhancing the binding and biological effects of LKT.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
authors.forename: F
authors.initials: F
authors.lastname: Leite
authors.forename: D
authors.initials: D
authors.lastname: Atapattu
authors.forename: C
authors.initials: C
authors.lastname: Kuckleburg
authors.forename: R
authors.initials: R
authors.lastname: Schultz
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology* / Q000821:virology*; D017077:Culture Media, Conditioned; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D005098:Exotoxins / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006566:Herpesviridae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007375:Interleukin-1 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D016978:Mannheimia haemolytica / Q000276:immunology*; D016871:Pasteurellaceae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D017077:Culture Media, Conditioned; D005098:Exotoxins; D007375:Interleukin-1; D016169:Lymphocyte Function-Associated Antigen-1; C018579:leukotoxin