title: [The expression pattern of L-type voltage-gated Ca2+ channel in rat cochlea].
abstract: OBJECTIVE
  To investigate the molecular basis of the voltage-gated Ca2+ channel (VGCC) which controls the afferent synaptic neurotransmitters release in inner hair cell and their cellular expression.
  
  
  METHODS
  In this study we used different and complementary approaches to determine which types of 4 subunits of VGCC were expressed in the organ of Corti.
  
  
  RESULTS
  RT-PCR analysis showed that alpha1C and alpha1D were present in the organ of Corti total RNA extract, and the corresponding antisense riboprobes could detect the expression of alpha1C and alpha1D in IHCs and OHCs.
  
  
  CONCLUSIONS
  The voltage-gated Ca2+ channel expressed in sensory cell in organ of Corti are alpha1C and alpha1D subtypes.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, PLA General Hospital, Beijing, 100853, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Shen
authors.forename: Yinyan
authors.initials: Y
authors.lastname: Hu
authors.forename: Weiyan
authors.initials: W
authors.lastname: Yang
authors.forename: Dongyi
authors.initials: D
authors.lastname: Han
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000235:genetics* / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism; D005260:Female; D006198:Hair Cells, Auditory / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009435:Synaptic Transmission
chemical_list: D020746:Calcium Channels, L-Type; D012333:RNA, Messenger