title: Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli.
abstract: Six of 37 non-O157 Escherichia coli strains possessing Shiga toxin (Stx) 2 gene variant stx(2d) or stx(2e) secreted no detectable Stx. These isolates produced significantly less stx mRNA than Stx2d, Stx2e, Stx2c, or Stx2 secretors did. Standard screening procedures miss a significant subset of E. coli harboring stx(2) variants.
authors.affiliation: Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch-Str. 41, 48149 Münster, Germany.
authors.forename: Wenlan
authors.initials: W
authors.lastname: Zhang
authors.forename: Martina
authors.initials: M
authors.lastname: Bielaszewska
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Friedrich
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kuczius
authors.forename: Helge
authors.initials: H
authors.lastname: Karch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D022641:Shiga Toxin 2 / Q000235:genetics* / Q000378:metabolism / Q000633:toxicity*; D014158:Transcription, Genetic; D014709:Vero Cells
chemical_list: D022641:Shiga Toxin 2