Specific distribution in homobasidiomycete hymenophores of the transcripts of Ras protein and G-protein alpha-subunit genes.
Results of in situ RNA-RNA hybridization showed a complementary distribution of the transcripts of Ras protein gene (ras) and trimeric G-protein alpha-subunit gene (ga1) in the hymenophores of white-rot basidiomycete Lentinula edodes and ectomycorrhizal basidiomycete Lyophyllum shimeji. The ras gene is present mostly in outer region of trama (the region branching out into the subhymenium) and in trams cells, while the ga1 gene is present mostly in hymenium (on which a large number of basidiospores are formed) and in subhymenium (on the top of which hymenium is formed). This suggests that the ras and ga1 genes play distinct physiological roles in the hymenophores of both basidiomycete fungi.
Tanaka
Yoshinori
Y
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Kaneko
Shinya
S
Katsukawa
Shiho
S
Yamazaki
Takashi
T
Ohta
Akira
A
Miyazaki
Yasumasa
Y
Shishido
Kazuo
K
D000363:Agaricales / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D044385:GTP-Binding Protein alpha Subunits / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015388:Organelles / Q000737:chemistry / Q000378:metabolism; D012331:RNA, Fungal / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D020942:Shiitake Mushrooms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018631:ras Proteins / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D004271:DNA, Fungal; D044385:GTP-Binding Protein alpha Subunits; D012331:RNA, Fungal; D012333:RNA, Messenger; D018631:ras Proteins