title: Leaf axil sampling of midwest U.S. maize for mycotoxigenic Fusarium fungi using PCR analysis.
abstract: PCR analysis was used to detect Fusarium species generically, as well as the mycotoxin-producing species F. subglutinans, F. proliferatum, and F. verticillioides in leaf axil and other maize tissues during ear fill in a multiyear study in central Illinois. The frequency of Fusarium detected varied from site to site and year to year. Fusarium was generically detected more frequently in leaf axil material than in leaf/husk lesions. In two growing seasons, the leaf axil samples were also tested for the presence of the mycotoxin producing species F. proliferatum, F. subglutinans, and F. verticillioides. Overall, F. proliferatum and F. verticillioides were detected less often than F. subglutinans. Fusarium was generically and specifically detected most commonly where visible fungal growth was present in leaf axil material.
authors.affiliation: U.S.D.A., Agricultural Research Service, National Center for Agricultural Utilization Research, Crop Bioprotection Research Unit, 1815 N. University St., Peoria, IL 61614, USA. dowdpf@mail.ncaur.usda.gov
authors.forename: Patrick F
authors.initials: PF
authors.lastname: Dowd
authors.forename: C Jason
authors.initials: CJ
authors.lastname: Barnett
authors.forename: Eric T
authors.initials: ET
authors.lastname: Johnson
authors.forename: James J
authors.initials: JJ
authors.lastname: Beck
mesh_terms: D005670:Fusarium / Q000145:classification* / Q000235:genetics; D009183:Mycotoxins / Q000032:analysis* / Q000096:biosynthesis; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction; D014481:United States; D003313:Zea mays / Q000382:microbiology*
chemical_list: D009183:Mycotoxins