title: Evolution of self-reactive IgG antibody repertoires in patients with relapsing-remitting multiple sclerosis.
abstract: We have previously demonstrated a distortion of self-reactive IgG antibody repertoires in patients with multiple sclerosis (MS) compared to controls, by immunoblotting assays, using human brain homogenates. The analysis of the immune profiles against human brain antigens allowed us to distinguish MS patients, and to associate a particular pattern of reactivity for each clinical form of MS. The aim of the present study was to evaluate the evolution of such patterns in patients with relapsing-remitting MS (RRMS). In a first step, we confirmed, by western blotting using human brains as source of antigens, the existence of specific repertoires of IgG reactivity in whole serum collected from healthy subjects (n = 32) and from untreated patients with RRMS (n = 56). In a second step, the evaluation of patterns was performed at baseline and 1 year later in untreated RRMS patients (n = 15), and in RRMS patients treated with IFN-beta (n = 41). In both groups, little change in IgG reactivity in whole serum was found. However, a higher degree of stability was noted in treated versus untreated patients (P < 0.01). Our results have showed a specific and relatively stable pattern of reactivity for each RRMS individual tested against brain antigens even after a 1-year treatment prevailing in treated patients suggesting that IFN-beta could stabilize IgG antibody repertoires.
authors.affiliation: Laboratoire d'immunologie EA2686, Faculté de Médecine, 1, Place de Verdun, 59045 Lille Cedex, France.
authors.forename: Almeras
authors.initials: A
authors.lastname: Lionel
authors.forename: J
authors.initials: J
authors.lastname: de Seze
authors.forename: Lefranc
authors.initials: L
authors.lastname: Didier
authors.forename: Fily-Nalewajk
authors.initials: FN
authors.lastname: Sandrine
authors.forename: Dubucquoi
authors.initials: D
authors.lastname: Sylvain
authors.forename: Vermersch
authors.initials: V
authors.lastname: Patrick
authors.forename: Prin
authors.initials: P
authors.lastname: Lionel
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D001921:Brain / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G