Regulation of hyaluronan synthases in mouse uterine cervix.
We examined the expression pattern of hyaluronan synthase (HAS) mRNAs in the uterine cervix of pregnant mice. The expression levels of HAS-1 and -2 mRNAs peaked at delivery, whereas that of HAS-3 mRNA peaked on the 15th day of pregnancy. The regulation of HAS mRNA expression was examined in pregnant mouse uterine cervical fibroblasts. The expression of HAS-1, -2, and -3 mRNAs was significantly augmented by interleukin-1beta (IL-1beta). Progesterone significantly interfered with expression of HAS-1 and -2 mRNAs, but significantly increased the expression of HAS-3 mRNA. Low-molecular-weight hyaluronan significantly enhanced only the expression of HAS-1 mRNA. These results indicate that HAS in the uterine cervix is regulated in a complex manner by IL-1beta, progesterone, and low-molecular-weight hyaluronan, of which changes in the cervical tissue and serum closely participate in uterine cervical ripening and/or inflammation.
Uchiyama
Taro
T
Shiseido Co. Ltd., Pharmaceutical Development Center, Japan. taro.uchiyama@to.shiseido.co.jp
Sakuta
Tomohiro
T
Kanayama
Toshiji
T
D000818:Animals; D001483:Base Sequence; D002584:Cervix Uteri / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D044967:Serum / Q000187:drug effects / Q000378:metabolism; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
D007375:Interleukin-1; D012333:RNA, Messenger; D011374:Progesterone; D006820:Hyaluronic Acid; D014166:Transferases; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases