title: Temperature dependence of the melittin folding equilibrium studied by means of fluorescence excitation spectra.
abstract: The temperature dependence of fluorescence excitation spectra is investigated. This gives information on melittin folding equilibrium complementary to that obtained from fluorescence emission studies. A fit of the excitation spectra of melittin with three Gaussian functions gives good results. It suggests that in melittin a reversal of La and Lb electronic states of tryptophane takes place. The separate La and Lb bands exhibit a temperature dependence of the width and maximum.
authors.affiliation: Laser Centre VU, Faculty of Sciences, Vrije Universiteit, Department of Analytical Chemistry and Applied Spectroscopy, De Boelelaan 1083, Amsterdam, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Smoluch
authors.forename: M
authors.initials: M
authors.lastname: Gorseling
authors.forename: C
authors.initials: C
authors.lastname: Gooijer
authors.forename: G
authors.initials: G
authors.lastname: van der Zwan
mesh_terms: D008555:Melitten / Q000737:chemistry*; D017510:Protein Folding; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D008555:Melitten