Comparison of Hsc70 orthologs from polar and temperate notothenioid fishes: differences in prevention of aggregation and refolding of denatured proteins.
Although a great deal is known about the cellular function of molecular chaperones in general, very little is known about the effect of temperature selection on the function of molecular chaperones in nonmodel organisms. One major unanswered question is whether orthologous variants of a molecular chaperone from differential thermally adapted species vary in their thermal responses. To address this issue, we utilized a comparative approach to examine the temperature interactions of a major cytosolic molecular chaperone, Hsc70, from differently thermally adapted notothenioids. Using in vitro assays, we measured the ability of Hsc70 to prevent thermal aggregation of lactate dehydrogenase (LDH). We further compared the capacity of Hsc70 to refold chemically denatured LDH over the temperature range of -2 to +45 degrees C. Hsc70 purified from the temperate species exhibited greater ability to prevent the thermal denaturation of LDH at 55 degrees C compared with Hsc70 from the cold-adapted species. Furthermore, Hsc70 from the Antarctic species lost the ability to competently refold chemically denatured LDH at a lower temperature compared with Hsc70 from the temperate species. These data indicate the function of Hsc70 in notothenioid fishes maps onto their thermal history and that temperature selection has acted on these molecular chaperones.
Place
Sean P
SP
Department of Ecology, Evolution, and Marine Biology, University of California, Santa Barbara, USA. place.sean@mayo.edu
Hofmann
Gretchen E
GE
D000818:Animals; D000864:Antarctic Regions; D004777:Environment; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000502:physiology; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D010473:Perciformes / Q000502:physiology*; D011489:Protein Denaturation / Q000502:physiology; D017510:Protein Folding; D020033:Protein Isoforms; D020673:Protein Renaturation; D013696:Temperature; D013997:Time Factors
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D020033:Protein Isoforms; D007770:L-Lactate Dehydrogenase