Synthesis of novel fluorogenic L-Fmoc lysine derivatives as potential tools for imaging cells.
Two coumarin-labeled lysines were conveniently prepared as fluorescent probes. 7-Methoxy and 7-diethylamino coumarin-3-carboxylic acids were synthesized according to a modification of known procedures. Labeling at lysine was achieved in solution via the active N-hydroxysuccinimide ester of the carboxylic acid coumarin derivatives to give the target compounds in good yield. Spectroscopic data (UV-vis and fluorescence) were recorded for all compounds.
Berthelot
Thomas
T
Groupe de Chimie Bio-Organique, INSERM U577, Université Victor Segalen Bordeaux 2, France.
Laïn
Georges
G
Latxague
Laurent
L
Déleris
Gérard
G
D000596:Amino Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D002477:Cells / Q000378:metabolism; D005449:Fluorenes / Q000138:chemical synthesis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008239:Lysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D005449:Fluorenes; D005456:Fluorescent Dyes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D008239:Lysine