title: [Coxsackie virus B types were discriminated by RT-PCR].
abstract: OBJECTIVE
  To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.
  
  
  METHODS
  A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.
  
  
  RESULTS
  This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.
  
  
  CONCLUSIONS
  The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.
authors.affiliation: Laboratory of Chemobiology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China. zhenyongl@sabc.com.cn
authors.forename: Zhen-yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Zhi-tao
authors.initials: ZT
authors.lastname: Li
authors.forename: Yan-ming
authors.initials: YM
authors.lastname: Feng
authors.forename: Da-xiao
authors.initials: DX
authors.lastname: Shao
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Zhao
authors.forename: Tian-xing
authors.initials: TX
authors.lastname: Cui
authors.forename: Guo-cui
authors.initials: GC
authors.lastname: Yang
authors.forename: Ling-bo
authors.initials: LB
authors.lastname: Qu
authors.forename: Yu-fen
authors.initials: YF
authors.lastname: Zhao
mesh_terms: D017931:DNA Primers; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D007075:Immunoglobulin M