title: [The development and collagen ingredients analysis of submandibular gland acellular matrix].
abstract: OBJECTIVE
  To develop submandibular gland acellular matrix and observe its structure and analyze its histological ingredients.
  
  
  METHODS
  The fresh submandibular glands of SD mice were cell-extracted by chemical detergent and histological ingredients were analyzed by immunohistochemistry.
  
  
  RESULTS
  Submandibular gland cells disappeared, matrix ingredients appeared network structure under microscopy. I, II and III type collagen proteins of submandibular gland before and after cell-extracted were positive under immunohistochemistry.
  
  
  CONCLUSIONS
  The main ingredients of submandibular gland acellular matrix were I, II and III type collagen proteins.
authors.affiliation: Department of Oral and Maxillofacial Surgery, First Clinical Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Chun-fu
authors.initials: CF
authors.lastname: Deng
authors.forename: Xue-xin
authors.initials: XX
authors.lastname: Tan
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Shao-hui
authors.initials: SH
authors.lastname: Huang
authors.forename: Zhong-fei
authors.initials: ZF
authors.lastname: Xu
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Wang
mesh_terms: D062088:Acellular Dermis / Q000378:metabolism*; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D013363:Submandibular Gland / Q000378:metabolism*
chemical_list: D003094:Collagen