Increasing digesta viscosity using carboxymethylcellulose in weaned piglets stimulates ileal goblet cell numbers and maturation.
Intestinal mucin, a family of glycoproteins secreted by goblet cells, is the main constituent of the mucus protecting the gastrointestinal tract. For optimal mucosal protection, both the quantitative and qualitative characteristics of mucin are essential. To evaluate how viscosity influences ileal apparent digestibility and mucin biology, a highly viscous nonfermentable soluble polysaccharide, carboxymethylcellulose (CMC), was fed to weaned piglets for 15 d. The ileal crude mucin concentration was determined by ethanol precipitation, and changes in goblet cell subtypes were analyzed by the histochemistry of ileal and colonic tissues. As expected, CMC increased the viscosity of ileal digesta and the moisture of feces (P < 0.001). The crude mucin concentration and output at the ileum were higher (P < 0.05) in pigs fed CMC than those fed the control diet. Increasing intestinal content viscosity in pigs fed CMC had no significant effects on the ileal apparent digestibility of dry matter, organic matter, nitrogen, and minerals. The number of total ileal goblet cells per villus also was higher (+30%, P < 0.05) in pigs fed the CMC diet compared with controls. This increase was essentially accounted for by increased numbers of acidic and acidic sulfated mucin-containing cells (+30%, P < 0.05). Trends (P = 0.06) toward decreased numbers of neutral and acidic mucin-containing cells in ileal crypts were also noted. In conclusion, increasing intestinal content viscosity in weaned piglets fed CMC increased the ileal mucin output and numbers and maturation of goblet cells in ileal villi without effects on the apparent digestibility of the diet.
Piel
Christelle
C
Unité Mixte de Recherche sur le Veau et le Porc, INRA-Agrocampus Rennes, Domaine de la Prise, 35590 Saint-Gilles, France.
Montagne
Lucile
L
Sève
Bernard
B
Lallès
Jean-Paul
JP
D000375:Aging; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002452:Cell Count; D004032:Diet; D004063:Digestion / Q000502:physiology*; D007082:Ileum / Q000254:growth & development; D007413:Intestinal Mucosa / Q000166:cytology* / Q000254:growth & development*; D013552:Swine; D014783:Viscosity; D014886:Weaning
D000596:Amino Acids