[Experimental studies on the expansion of cord blood CD34+ cells and the improvement of their grafting efficiency].
OBJECTIVE
To elucidate the expanding response of cord blood CD34+ cells to human hematopoietic growth factors (HGFs) and evaluate their grafting efficiency.


METHODS
CD34+ cells were isolated from umbilical cord blood by using a high-gradient magnetic cell sorting system (MACS), expanded with HGFs in a liquid culture system and evaluated of grafting efficiency with a panning assay on an adult bone marrow stroma.


RESULTS
The percentage of CD34+ CD38- subset in cord blood was much higher than that in bone marrow or peripheral blood. Treatment with a combination of HGFs, including SCF, IL-3, IL-6, G-CSF,and Epo increased hematopoietic progenitor cells by nearly 100 fold and nucleated cells by more than 500 fold. 68.0%-89.6% of the CD34+ cells treated with HGFs (GM-CSF or IL-3, alone or in combination) seeded in the pre-irradiated marrow stroma, whereas only 36% of the untreated ones did.


CONCLUSIONS
It is possible to expand cord blood CD34+ cells ex vivo and to improve their grafting efficiency with HGFs.
Pei
X
X
Beijing Institute of Radiation Medicine, Beijing.
Wang
L
L
Xu
L
L
Wu
Z
Z
D018952:Antigens, CD34 / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D036101:Cord Blood Stem Cell Transplantation; D005312:Fetal Blood / Q000166:cytology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006801:Humans
D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors