title: Characterization of allergen-specific monocyte-derived dendritic cells generated from monocytes by a single-step procedure: effect on naïve and memory T cells.
abstract: BACKGROUND
  Dendritic cells are one of the most potent antigen-presenting cells and when pulsed with allergen can modulate allergen-specific T-cell responses. We sought to establish a single-step method by which to generate allergen-specific monocyte-derived dendritic cells (MoDCs).
  
  
  METHODS
  Dermatophagoides farinae (Df)-prepulsed MoDCs were generated from monocytes by culturing with Df in the presence interleukin (IL)-4, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumour necrosis factor (TNF)-alpha simultaneously. Df-prepulsed MoDC were incubated with autologous naive and memory T cells in the absence of recall antigen, then proliferation and cytokine production by T cells was determined.
  
  
  RESULTS
  Generation of allergen-prepulsed MoDCs was confirmed by examining expression of surface molecules. Df-prepulsed MoDC selectively induced proliferation of Df-specific T cells in the absence of recall antigen. Under these conditions, Df-prepulsed MoDCs augmented but did not alter the cytokine production profile. In addition, Df-prepulsed MoDCs activated naive T cells leading to proliferation and selective production of IFN-gamma in allergic patients but not in healthy subjects.
  
  
  CONCLUSIONS
  These results suggest that Df-prepulsed MoDC generated from monocytes by a simple single-step manipulation can induce Df-specific cellular responses from both naive and memory T cells in the absence of recall antigen, and these cells potentially can be utilized as immune adjuvants in allergen-specific immunotherapy.
authors.affiliation: Department of Otolaryngology - Head & Neck Surgery, Okayama University Graduate Schoolof Medicine and Dentistry, Okayama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takishita
authors.forename: M
authors.initials: M
authors.lastname: Okano
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
authors.forename: Y
authors.initials: Y
authors.lastname: Sugata
authors.forename: H
authors.initials: H
authors.lastname: Hattori
authors.forename: S
authors.initials: S
authors.lastname: Ohuchi
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Nishizaki
mesh_terms: D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D000954:Antigens, Surface / Q000378:metabolism; D016022:Case-Control Studies; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis; D003584:Cytological Techniques; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D009000:Monocytes / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D039741:Antigens, Dermatophagoides; D000954:Antigens, Surface; D016207:Cytokines