title: Scarce evidence of yogurt lactic acid bacteria in human feces after daily yogurt consumption by healthy volunteers.
abstract: In a double-blind prospective study including 114 healthy young volunteers, the presence in human feces of the yogurt organisms Lactobacillus delbrueckii and Streptococcus thermophilus after repeated yogurt consumption (15 days) was analyzed by culture, specific PCR, and DNA hybridization of total fecal DNA. Detection of yogurt lactic acid bacteria in total fecal DNA by bacterial culture and PCR assay was consistently negative. DNA compatible with yogurt bacteria was found by hybridization experiments in only 10 (10.52%) of 96 individuals after consumption of fresh yogurt and in 2 (2.10%) of 96 individuals after consumption of pasteurized yogurt (P = 0.01).
authors.affiliation: Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Ctra. Colmenar, Km 9.1, Madrid 28.034, Spain. rosacampo@yahoo.com
authors.forename: Rosa
authors.initials: R
authors.lastname: del Campo
authors.forename: Daniel
authors.initials: D
authors.lastname: Bravo
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
authors.forename: Patricia
authors.initials: P
authors.lastname: Ruiz-Garbajosa
authors.forename: Raimundo
authors.initials: R
authors.lastname: García-Albiach
authors.forename: Alejandra
authors.initials: A
authors.lastname: Montesi-Libois
authors.forename: Francisco-Javier
authors.initials: FJ
authors.lastname: Yuste
authors.forename: Victor
authors.initials: V
authors.lastname: Abraira
authors.forename: Fernando
authors.initials: F
authors.lastname: Baquero
mesh_terms: D000328:Adult; D015169:Colony Count, Microbial; D003470:Culture Media; D004311:Double-Blind Method; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D048668:Lactobacillus delbrueckii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D019936:Probiotics; D011446:Prospective Studies; D048249:Streptococcus thermophilus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015014:Yogurt / Q000382:microbiology*
chemical_list: D003470:Culture Media