Tea polyphenol epigallocatechin-3-gallate associates with plasma membrane lipid rafts: lipid rafts mediate anti-allergic action of the catechin.
High-affinity IgE receptor FcepsilonRI is key molecule in the IgE-mediated allergic reactions. Epigallocatechin-3-gallate (EGCG) has a suppressive effect of the expression of the FcepsilonRI. We show here that EGCG highly associates with plasma membrane microdomains, lipid rafts. The disruption of these lipid rafts caused a reduction of the amount of raft-associated EGCG and the FcepsilonRI -suppressive effect of EGCG. These results suggest that the interaction between EGCG and the lipid rafts is important for EGCG's ability to downregulate FcepsilonRI expression.
Tachibana
Hirofumi
H
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. tatibana@agr.kyushu-u.ac.jp
Fujimura
Yoshinori
Y
Yamada
Koji
K
D000975:Antioxidants / Q000494:pharmacology*; D001491:Basophils / Q000187:drug effects / Q000276:immunology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D006634:Histamine H1 Antagonists; D006801:Humans; D021962:Membrane Microdomains / Q000187:drug effects / Q000502:physiology*; D017455:Receptors, IgE / Q000187:drug effects*; D013662:Tea
D000975:Antioxidants; D006634:Histamine H1 Antagonists; D017455:Receptors, IgE; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate