[Protective effects of Cleistocalyx operculatus on lipid peroxidation and trauma of neuronal cells].
OBJECTIVE
To observe the protective effects of Cleistocalyx operculatus on lipid peroxidation in rat liver microsomes and on the trauma of PC12 cells induced by H2O2.


METHODS
The mouse liver homogenate lipid peroxidation assay and PC12 Cell culture and Cell viability (MTT assay) were applied.


RESULTS
Cleistocalyx operculatus showed strong protective effects on lipid peroxidation in rat liver microsomes in a dose-dependent manner and exhibited potent protective effects on the trauma of PC12 cells induced by H2O2 (200 micromol x L(-1)) when the concentration reached 1.00 g x L(-1).


CONCLUSIONS
Cleistocalyx operculatus may be used as antioxidant to prevent or delay the pathogenesis of neural cell diseases.
Lu
Yan-hua
YH
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China.
Du
Chang-bin
CB
Wu
Zi-bin
ZB
Ye
Chun-lin
CL
Liu
Jian-wen
JW
Wei
Dong-zhi
DZ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D019610:Cytoprotection / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D027822:Myrtaceae / Q000737:chemistry; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D006861:Hydrogen Peroxide