[Achievements and applications in making chicken chimeras using BCs].
The technology of producing chicken chimeras using blastodermal cells is very important not only in the field of transgenic chicken bioreactor but also in searching for efficient ways to conserve avian genetic resource. The basic processes for producing chicken chimeras consist of: (1) Setting up the color model; (2) Separating and dissociating of donor embryos; (3) Compromising of the recipient embryos; (4) Windowing and recovering the recipient eggs; (5) Cells injecting; (6) Method of hatching. The progress, obstacles and prospects of producing chicken chimeras via BCs were discussed in this paper.
Yan
Hai-Feng
HF
Hunan Institute of Animal and Veterinary Science, Changsha 410 131, China. hfklagh@public.cs.hn.cn
Shao
Bing-Nan
BN
Trefil
P
P
Guo
Xiang-Xia
XX
Wu
Xiao-Lin
XL
D000818:Animals; D030801:Animals, Genetically Modified; D001756:Blastoderm / Q000166:cytology*; D017690:Cell Transplantation; D002642:Chick Embryo / Q000502:physiology; D002645:Chickens / Q000235:genetics*; D002678:Chimera / Q000235:genetics*; D004528:Egg Shell