[Identification of ESTs of genes related to compaction in preimplantation embryos of mouse].
A total of 181 8-cell embryos and 241 8-cell compacted embryos were collected respectively from Kunmingbai mouse and their cDNA was synthesized directly using SMART PCR. Genes, which expressed differently between early 8-cell embryos and 8-cell compacted embryos, were investigated using the method of suppression subtractive hybridization (SSH). Then PCR production was cloned into pUCm-T vector respectively according to the size after isolated and purified. Twenty-seven ESTs (expressed sequence tags) of genes expressed differently between early 8-cell embryos and 8-cell compacted embryos have been isolated and cloned. seventeen of those were novel ESTs after being confirmed by blaster matching in GenBank for homology analysis. And they were banked into GenBank with accession numbers. All 17 ESTs might be for novel genes related to compaction in compacted embryos. And longer ESTs may be obtained by cloning according to the size.
Li
Wen
W
The Human Reproductive Engineering Lab of Xiangya College of Southern Centre University, Changsha 410 078, China. wenshui14@sina.com
Lu
Guang-Xiu
GX
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy
D018076:DNA, Complementary