Heat-accelerated fixation and rapid dissection of the pediatric brain at autopsy: a pragmatic approach to the difficulties of organ retention.
We investigated whether it is possible to accelerate the examination of a pediatric brain at autopsy and thus facilitate its return to the body before a funeral without compromising the quality of the neuropathologic examination. Accelerated fixation and next-day dissection of the brain was performed in selected cases over a 2-year period by using a microwave histologic tissue processor (MicroMed T/T MEGA, Milestone, Sorisole, Italy). Direct comparison of the histologic appearance and immunohistochemical reactivity of 2 cases, 1 fixed by conventional methods and 1 fixed with the accelerated method, was performed in a blinded fashion by a specialist neuropathologist. Examination of rapidly fixed brain by conventional thin coronal sections was readily achieved. There was no appreciable difference between tissue sections stained with hematoxylin and eosin and prepared from conventional formalin-fixed cortical and cerebellar brain tissue and that fixed by rapid heat acceleration. Immunocytochemical studies were not adversely affected by the accelerated heat-fixation process of tissue. Heat-accelerated fixation is a potential method of speeding up the examination of the brain at autopsy without unduly compromising the quality of the neuropathologic examination.
Barrett
Ciara
C
Department of Histopathology, Our Lady's Hospital for Sick Children, Crumlin, Dublin, Ireland.
Brett
Francesca
F
Grehan
David
D
McDermott
Michael B
MB
D000293:Adolescent; D001344:Autopsy / Q000379:methods; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D004210:Dissection / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008872:Microwaves; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods*