[The cloning and sequence analysis of cDNA of the partial E genomic region of Dengue type 4 virus from a Chinese patient].
The partial envelope (E) protein gene of Dengue virus type 4 (DV4), derived from the serum of a patient with dengue fever in an epidemic during October 1993 in Guangdong Province of China, was cloned and sequenced. DV RNA was converted to cDNA by reverse transcription with random primers before the polymerase chain reaction using DV4 specific primers was performed. The amplified product of 421bp was subsequently filled in 3' recessed ends, isolated, purified and inserted into pUC18 and pUC19 plasmid vector. Their sequences were determined by dideoxy nucleotide chain termination method. A comparison between DV4 Chinese strain and several other previously reported strains shows the homology to be 93.72% for the same dengue serotype, 61.26-64.40% for the other dengue serotypes, only 40.31% for Japanese encephalitis virus, which belongs to the member of flavivirus family. A conserved sequence with 12 amino acids observed from deduced protein sequence probably represents an essential functional element among flavivirus family.
Li
G
G
Department of Infectious Diseaes, Sun Yatsen University of Medical Sciences, Guangzhou 510630.
Yao
J
J
Peng
W
W
Wang
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics*; D003715:Dengue / Q000235:genetics / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics*; D055815:Young Adult
D018076:DNA, Complementary; D014759:Viral Envelope Proteins; C466981:glycoprotein E, dengue virus type 4