title: Varicosity-Schwann cell interactions mediated by ATP in the mouse vas deferens.
abstract: Schwann cells, from a variety of sources, are known to possess P2Y purinergic metabotropic receptors. However, it is not known if Schwann cells associated with autonomic nerve terminals possess such receptors and if so whether these receptors are activated by the endogenous release of ATP from the nerve terminals. We show that such Schwann cells in the vas deferens give evoked calcium transients on nerve stimulation. These transients are mediated, at least in part, by the endogenous release of ATP, which acts on Schwann cell P2Y receptors to release calcium from within the cells. This work suggests the possibility that Schwann cells are active participants in the process of junctional transmission in the autonomic nervous system.
authors.affiliation: The Neurobiology Laboratory, Department of Physiology and Institute for Biomedical Research, University of Sydney, NSW, Australia.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Lin
authors.forename: M R
authors.initials: MR
authors.lastname: Bennett
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000320:Adrenergic Fibers / Q000528:radiation effects; D000814:Aniline Compounds; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002452:Cell Count / Q000379:methods; D004347:Drug Interactions; D004558:Electric Stimulation / Q000379:methods; D004963:Estrenes / Q000494:pharmacology; D005071:Evoked Potentials / Q000187:drug effects; D005456:Fluorescent Dyes / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009930:Organic Chemicals; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology; D011799:Quinazolines / Q000494:pharmacology; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000187:drug effects* / Q000502:physiology; D013498:Suramin / Q000494:pharmacology; D013997:Time Factors; D014230:Triazoles / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology; D014649:Vas Deferens / Q000166:cytology* / Q000187:drug effects; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D004963:Estrenes; D005456:Fluorescent Dyes; C403124:Oregon Green BAPTA-dextran; D009930:Organic Chemicals; D010726:Phosphodiesterase Inhibitors; D010975:Platelet Aggregation Inhibitors; D011760:Pyrrolidinones; D011799:Quinazolines; D009418:S100 Proteins; D014230:Triazoles; D014966:Xanthenes; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; C059715:Fluo-3; D002110:Caffeine; D011732:Pyridoxal Phosphate; D013498:Suramin; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate; C052410:9-chloro-2-(2-furyl)-(1,2,4)triazolo(1,5-c)quinazolin-5-imine