title: [Effect of danshensu, protocatechualdehyde and danshen injection on calcium ion concentration in cytoplasm of human erythrocytes].
abstract: OBJECTIVE
  To study the predominant calcium-antagonist components of Danshen injection.
  
  
  METHODS
  The effects of danshensu, protocatechualdehyde and Danshen injection on calcium concentration in cytoplasm of erythrocytes were examined in vitro by the fluorescent Ca+ -chelator fura-2.
  
  
  RESULTS
  Either DS182 or PCAD can decrease in dose-dependent cytosolic free calcium concentration in human erythrocytes. They had additive effect when mixed, which was similar to Danshen injection.
  
  
  CONCLUSIONS
  DS182 and PCAD may be predominant calcium-antagonist components of Danshen injection.
authors.affiliation: Department of Cadiology, Renji Hospital of Shanghai Second Medical University, Shanghai 200001, China. shenlinghong@medmail.com.cn
authors.forename: Ling-hong
authors.initials: LH
authors.lastname: Shen
authors.forename: Bin-yao
authors.initials: BY
authors.lastname: Wang
authors.forename: Chang-qian
authors.initials: CQ
authors.lastname: Wang
authors.forename: Xiu-lan
authors.initials: XL
authors.lastname: Xie
authors.forename: Guo-rui
authors.initials: GR
authors.lastname: Yu
authors.forename: Zhong-yi
authors.initials: ZY
authors.lastname: Yao
authors.forename: Yuan-long
authors.initials: YL
authors.lastname: Zhu
authors.forename: Bao-jin
authors.initials: BJ
authors.lastname: Yang
mesh_terms: D000328:Adult; D001547:Benzaldehydes / Q000302:isolation & purification / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002396:Catechols / Q000302:isolation & purification / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007267:Injections; D007773:Lactates / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D010946:Plants, Medicinal / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; D002396:Catechols; D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde; D002118:Calcium