[Tumor necrosis factor-alpha gene polymorphisms and risk of coronary heart disease].
Tumor necrosis factor-alpha(TNF-alpha) is a cytokine that has multiple functions. It could be involved in cardiovascular pathophysiology. Given this possibility, we measured serum TNF-alpha level by ELISA and use PCR-RFLP methods to examine the distribution of TNF-alpha locus gene polymorphism at -857 and -863 sites in coronary heart disease(CHD) patients and matched controls. We found the serum level of TNF-alpha of CHD is higher than that of controls(P<0.05),-863 genotype and allele have significant difference between two groups(P<0.05),and there are no difference in two groups at -857 site.
Xiang
Ping-Xia
PX
Department of Medical Laboratory Science, RenMin Hospital of Wuhan University, Wuhan 430060,China. estheresther@sina.com
Li
Yan
Y
Zhang
Ping-An
PA
D000368:Aged; D000483:Alleles; D003327:Coronary Disease / Q000097:blood / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha