Soybean oil fat emulsion prevents cytochrome P450 mRNA down-regulation induced by fat-free overdose total parenteral nutrition in infant rats.
Changes in the mRNA expression of hepatic cytochrome P450 (CYP) isoenzymes associated with overdose of fat-free or fat-containing total parenteral nutrition (TPN) were investigated in infant rats. Three-week-old male Sprague-Dawley rats were divided into three groups: group 1 received an oral diet, group 2 received TPN without fat, and group 3 received TPN with 20% of calories from fat (soybean oil emulsion). After TPN administration for 4 d, serum aspartate aminotransferase (AST) levels in group 2 were significantly increased (p<0.01) compared with the other groups. The mRNA expression of hepatic CYP isoenzymes in group 2 decreased to 0.76 to 31% of that in group 1 (p<0.01), but that in group 3 was maintained at 32 to 84% of that in group 1. These results indicate the importance of including fat in TPN regimens to prevent not only hepatic dysfunction but also mRNA down-regulation of liver CYP isoenzymes.
Yamaguchi
Mari
M
Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima, Japan.
Yamauchi
Aiko
A
Nishimura
Masuhiro
M
Ueda
Nobuhiko
N
Naito
Shinsaku
S
D000818:Animals; D000831:Animals, Newborn; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D018752:Diet, Fat-Restricted / Q000009:adverse effects* / Q000379:methods; D015536:Down-Regulation / Q000187:drug effects* / Q000502:physiology; D062787:Drug Overdose; D004655:Emulsions; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D010288:Parenteral Nutrition / Q000009:adverse effects* / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013024:Soybean Oil / Q000008:administration & dosage*
D004655:Emulsions; D007527:Isoenzymes; D012333:RNA, Messenger; D013024:Soybean Oil; D003577:Cytochrome P-450 Enzyme System