[Study on the response of specific antibodies against SARS-CoV in patients infected with SARS].
OBJECTIVE
To study the response of specific antibodies against severe acute respiratory syndrome (SARS)-CoV in patients infected with SARS.


METHODS
IgM-capture, indirect and antigen-sandwiched enzyme linked immunosorbent assay (ELISA) were used to detect the SARS-CoV specific IgM, IgG and total antibodies in sera of clinical SARS patients or non-SARS individuals.


RESULTS
The positive rates of IgM, IgG and total antibodies to SARS-CoV in 146 sera of SARS patients collected in different phases of the disease were 61.64%, 53.43% and 69.86%, respectively. The earliest detectable days after onset of the disease for IgM and IgG to SRAS-CoV were 7 and 12 days, respectively. The specific IgM disappeared as early as 42 days after the onset of SARS. Of 70 sera from hepatitis A patients, 2 showed false positive results, while 127 sera from other patients were all negative, detected by the 3 methods. Serum from one medical worker who had been close contact to SARS patients was positive for anti-SARS-CoV IgG and total antibodies. These 3 methods used for detection were all not influenced by rheumatoid factor (RF).


CONCLUSIONS
All of the three methods were specific and sensitive for the detection of specific antibodies to SARS-CoV, and useful for epidemiological research and clinical diagnosis, but not for early diagnosis of SARS.
Mao
Pan-yong
PY
Institute of Infectious Diseases, Hospital 302 of the Chinese People's Liberation Army, Beijing 100039, China.
Zhu
Lei
L
Wang
You-chun
YC
Hou
Jun
J
Hu
Yan
Y
Shen
Hong-hui
HH
Xin
Shao-jie
SJ
Li
Bao-sen
BS
Mao
Yuan-li
YL
Zhuang
Hui
H
D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology* / Q000821:virology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M