[Detection and typing for swine leukocyte antigen].
Traditionally the cluster of swine leukocyte antigen (SLA) was typed by serological, cytological and biochemical methods. Many special molecular typing methods have been developed with the progress of molecular biological technology, such as PCR-RFLP, PCR-SSCP , MS and DNA sequencing. Here we discussed the advantages and disadvantages of each method based on the polymorphic and conservative (from the functional aspect, such as supertype and supermotif) characteristics of SLA, and illustrated the development of typing for SLA in the future. In addition, we pointed out the editorial mistakes about the serological haplotype of SLA in reference book and emphasized that the accurate polymorphism of SLA-DQB gene must be based on the cloning sequencing.
Li
Hua
H
The Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunan 650 223, China. okhua@yahoo.com.cn
Luo
Huai-Rong
HR
Zhang
Ya-Ping
YP
Qiu
Xiang-Pin
XP
Ye
Chun
C
D000818:Animals; D006239:Haplotypes / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000145:classification / Q000235:genetics; D000949:Histocompatibility Antigens Class II; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics* / Q000276:immunology
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen