Isolation and molecular characterisation of equine rotaviruses from Germany.
A total of 26 rotavirus positive faecal samples of diarrhoeal foals, and 8 equine rotavirus isolates were examined. Viral RNA patterns were generated, G typing was performed by PCR, and a P[12]-specific DNA probe was developed for P typing. Furthermore, five equine rotavirus isolates were sequenced in the genomic regions coding for VP7 and part of VP4. Rotaviruses of genotype G3 P[12] were found in 22 faecal samples and G14 P[12] type could be found in 4 faecal samples. These findings confirm that in Germany G3 P[12] is the predominating type of equine rotaviruses.
Elschner
Mandy
M
Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, D-07743 Jena, Germany. m.elschner@jena.bfav.de
Schrader
Christina
C
Hotzel
Helmut
H
Prudlo
Jutta
J
Sachse
Konrad
K
Eichhorn
Werner
W
Herbst
Werner
W
Otto
Peter
P
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000662:veterinary; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D005838:Genotype; D005858:Germany; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins