[The polymorphism of nine STR loci on the genetic original identification of complete hydatidiform mole].
To explore the genetic origin of hydatidiform mole (CHM), 33 cases of CHM were collected mainly from Harbin Red Cross Central hospital from 1998.6 to 2001.5 and studied by multiplex-PCR, products were separated using denaturing polyacrylamide gel and were detected by silver stain for 9 different STR loci analysis. Among 33 samples of CHM, DNA from only paternal origin was found in 27 cases (81.8%, 27/33), and from both parents in 6 cases (18.2%, 6/33); and in the former, the homozygous CHM and the heterozygous CHM were 22 cases (66.7%, 22/33) and 5 cases (15.1%, 5/33), respectively. There was difference between analysis of microsatellite DNA polymorphism and pathological diagnosis in hydatidiform moles' classification. The results suggest that the analysis of 9 polymorphic STR loci is suitable for genetic original identification of hydatidiform moles.
Ou
Chun-Yi
CY
Harbin Red Cross Central Hospital, Harbin 150 076, China. chunyi_ou@163.com
Chen
Meng-Hua
MH
Niu
Ming-Gang
MG
D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006828:Hydatidiform Mole / Q000145:classification / Q000235:genetics* / Q000473:pathology; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm