title: [Analysis of clonality with immunoglobulin heavy chain gene rearrangement fingerprinting map in childhood acute lymphoblastic leukemia].
abstract: OBJECTIVE
  To investigate the types of the immunoglobulin heavy chain (IgH) gene rearrangement and the co-existing clones in childhood acute lymphoblastic leukemia (ALL).
  
  
  METHODS
  Polymerase chain reaction (PCR) amplification and single-strand conformation polymorphism (SSCP) were used to construct the IgH-PCR-SSCP gene fingerprinting.
  
  
  RESULTS
  Of the 34 gene fingerprinting maps obtained from newly diagnosed ALL, 9 (32.14%) were one allelic rearrangement and 15 (53.75%) were biallelic in mono-clonal rearrangements. Four cases (14.29%) with high white cell count and precursor-B immunophenotype were oligoclonal rearrangements.
  
  
  CONCLUSIONS
  There were multiple IgH bi-allelic rearrangements and co-existing clones in childhood ALL.
authors.affiliation: Department of Pediatrics, PLA General Hospital, Beijing 100853.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Lu
authors.forename: P
authors.initials: P
authors.lastname: Zhu
authors.forename: H
authors.initials: H
authors.lastname: Xue
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D005455:Fluorescent Antibody Technique; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016130:Immunophenotyping; D008297:Male; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology
chemical_list: D007143:Immunoglobulin Heavy Chains