Identification and distribution of endoplasmic reticulum iPLA2.
Our laboratory demonstrated that endoplasmic reticulum iPLA2 (ER-iPLA2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA2 isoform(s) responsible for ER-iPLA2 activity in different species and tissues. ER-iPLA2 activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA2gamma (group VIB PLA2) message in all tissues tested. Immunoblot analysis and PLA2 inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA2 in rabbit kidney and heart and rat kidney is iPLA2gamma. These results demonstrate the expression of ER-iPLA2gamma (group VIB) across species and tissues, and suggest that iPLA2gamma may play critical roles in oxidant-induced cell injury.
Kinsey
Gilbert R
GR
Department of Pharmaceutical Sciences, Medical University of South Carolina, 280 Calhoun, POB 250140, Charleston, SC 29425, USA.
Cummings
Brian S
BS
Beckett
Caroline S
CS
Saavedra
Geraldine
G
Zhang
Wenliang
W
McHowat
Jane
J
Schnellmann
Rick G
RG
D000595:Amino Acid Sequence; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D054522:Group VI Phospholipases A2; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D009281:Naphthalenes / Q000494:pharmacology; D009928:Organ Specificity; D063065:Organophosphonates / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D020033:Protein Isoforms / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011753:Pyrones / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity
D001095:Arachidonic Acids; D004791:Enzyme Inhibitors; D009281:Naphthalenes; D063065:Organophosphonates; D020033:Protein Isoforms; D011753:Pyrones; D012333:RNA, Messenger; C104810:methyl arachidonylfluorophosphonate; C068314:6-(bromomethylene)tetrahydro-3-(1-naphthaleneyl)-2H-pyran-2-one; D010741:Phospholipases A; D054522:Group VI Phospholipases A2; C516475:PLA2G6 protein, human; D054467:Phospholipases A2