Listeria's right-handed helical rocket-tail trajectories: mechanistic implications for force generation in actin-based motility.
Listeria monocytogenes forms right-handed helical rocket tail trajectories during actin-based motility in cell-free extracts, and this stereochemical feature is consistent with actoclampin's affinity-modulated, clamped-filament elongation model [Dickinson and Purich, 2002: Biophys J 82:605-617]. In that mechanism, right-handed torque is generated by an end-tracking molecular motor, each comprised of a filament barbed end and clamping protein that processively traces the right-handed helix of its filament partner. By contrast, torque is not a predicted property of those models (e.g., elastic propulsion, elastic Brownian ratchet, tethered ratchet, and insertional polymerization models) requiring filament barbed ends to depart/detach from the motile object's surface during/after each monomer-addition step. Helical trajectories also explain why Listeria undergoes longitudinal-axis rotation on a length-scale matching the helical periodicity of Listeria's rocket tails.
Zeile
William L
WL
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610-0245, USA.
Zhang
Fangliang
F
Dickinson
Richard B
RB
Purich
Daniel L
DL
D000199:Actins / Q000502:physiology*; D000818:Animals; D008089:Listeria monocytogenes / Q000502:physiology*; D009068:Movement / Q000502:physiology; D051381:Rats
D000199:Actins