title: He-Ne laser effects on blood microcirculation during wound healing: a method of in vivo study through laser Doppler flowmetry.
abstract: OBJECTIVE
  Low-intensity laser therapy (LILT) is widely used for wound healing promotion and its mechanism of action may be due to an enhancement of blood supply. The aim of this study was to evaluate blood flow alterations in a wound healing model, using laser Doppler flowmetry (LDF) associated with a normalized perfusion parameter.
  
  
  METHODS
  An injury was provoked in 15 rats and blood flow was measured periodically over a period of 21 days. Control groups were established to evaluate LDF and He-Ne laser effects on microcirculation. A 1 J/cm(2) dose was utilized, with 6 mW/cm(2) irradiance.
  
  
  RESULTS
  The results demonstrated flow alterations provoked by lesion, and inflammatory response (P < 0.05). There were no statistical differences between groups.
  
  
  CONCLUSIONS
  The results did not show a significant sustained effect on microcirculation with this He-Ne dose.
authors.affiliation: Centro de Lasers e aplicacoes-Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, Brazil 05508-000. silnunez@aol.com
authors.forename: Silvia Cristina
authors.initials: SC
authors.lastname: Núñez
authors.forename: Gessé E C
authors.initials: GE
authors.lastname: Nogueira
authors.forename: Martha S
authors.initials: MS
authors.lastname: Ribeiro
authors.forename: Aguinaldo S
authors.initials: AS
authors.lastname: Garcez
authors.forename: José L
authors.initials: JL
authors.lastname: Lage-Marques
mesh_terms: D000818:Animals; D017078:Laser-Doppler Flowmetry; D028022:Low-Level Light Therapy / Q000379:methods*; D008833:Microcirculation / Q000000981:diagnostic imaging / Q000528:radiation effects*; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D014463:Ultrasonography; D014945:Wound Healing / Q000528:radiation effects*