[The cloning and expression of a novel rPDCD5 gene from rice].
An EST related to the gene PCD was isolated from SSH (suppression subtractive hybridization) library of callus tissues of rice (Oryza sativa L.). Primers were designed to obtain its complete cDNA encoding putative apoptosis-related protein from Shanyou 63 (Oryza sativa L.). Sequencing indicated that the gene contained a 387 bp open reading frame, which encodes a protein containing 128 aa. Sequence alignment showed that the deduced protein is highly homologous to the known PDCD5. Real time quantitative PCR was performed to reveal that rPDCD5 was up-regulated in abiotic stress (low temperature and NaCl treatment).
Mi
Li-Ping
LP
Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China. mlp5155@sina.com
Wei
Chun
C
Huang
Jun-Sheng
JS
Du
Xi-Ling
XL
Qian
Xiao-Yin
XY
Li
Ke-Gui
KG
Shen
Guo-An
GA
Yang
Ji-Shui
JS
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D012965:Sodium Chloride