[Degradation of biomolecules: a comparative study of diagenesis of DNA and proteins in human bone tissue].
Diagenesis of macromolecules is a not yet fully understood process that can be important for anthropological and forensic research. Trying to elucidate the diagenesis of DNA and proteins we investigated the process of fragmentation of DNA and razemisation of aspartic acid in human bone material. We created an in vitro-model of accelerated aging by incubating bone samples in hot water. A comparison of diagenesis of molecules in those artificially aged samples with altogether 30 historical bones from different regions and of different ages was carried out. The in vitro-model showed the expected positive correlation between the increase of razemisation of aspartic acid and DNA fragmentation, while there was a much lesser correlation when investigating historical bones. The in vitro-model showed the expected correlation between the increase of razemisation of aspartic acid and DNA fragmentation and to a much lesser extent in historical bones. This study shows that diagenesis is probably influenced by additional forces affecting different macromolecules in different ways.
Harbeck
Michaela
M
Institut für Rechtsmedizin, Christian-Albrechts-Universität Kiel.
Ritz-Timme
Stefanie
S
Schröder
Inge
I
Oehmichen
Manfred
M
von Wurmb-Schwark
Nicole
N
D000365:Age Determination by Skeleton; D000591:Amino Acid Isomerases / Q000235:genetics*; D001224:Aspartic Acid / Q000235:genetics*; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D026603:D-Aspartic Acid / Q000235:genetics*; D005554:Forensic Medicine; D005819:Genetic Markers / Q000235:genetics*; D049690:History, Ancient; D006801:Humans; D066298:In Vitro Techniques; D046912:Multiprotein Complexes / Q000235:genetics*; D010164:Paleopathology; D016133:Polymerase Chain Reaction / Q000379:methods; D013237:Stereoisomerism; D020080:Tandem Repeat Sequences
D005819:Genetic Markers; D046912:Multiprotein Complexes; D001224:Aspartic Acid; D026603:D-Aspartic Acid; D000591:Amino Acid Isomerases