[Preparation and specificity analysis of anti-Drosophila Dxl6 antibody].
In order to study the function of Dxl6 which is a novel member of SR protein family, its cDNA was cloned by RT-PCR, and the sequences of its RS domain and its middle part were subcloned into two fusion express vectors, pGEX-4T-1His(6)C and pET32a. After expressing in E.coli BL21, the truncated proteins of Dxl6 RS domain part and Dxl6 middle part in pGEX-4T-1His(6)C were purified and used to immunize rabbits. Purified antibodies against the RS domain and Dxl6 middle part were obtained by affinity chromatography with the expressed products of Dxl6 RS domain part and Dxl6 middle part in pET32a, respectively. The result shows the antibody against Dxl6 RS domain has a good specificity to Dxl6 in Drosophila larvae by Western blot analysis.
Wang
Shan-Zhi
SZ
Genetic Research Center, Department of Genetics and Developmental Biology, Southeast University, Nanjing, Jiangsu 210 009, China. wsz.seu@163.net
Yuan
Liu-Di
LD
Wan
Yong-Qi
YQ
Liu
Li
L
Xie
Wei
W
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017344:Genes, Insect; D005822:Genetic Vectors; D007106:Immune Sera / Q000276:immunology; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000068103:Serine-Arginine Splicing Factors
D000906:Antibodies; D018076:DNA, Complementary; D007106:Immune Sera; D019476:Insect Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C106161:Srsf3 protein, mouse; D000068103:Serine-Arginine Splicing Factors