title: [Effects of ethyl pyruvate on cell-mediated immune function in rats with delayed resuscitation after burn injury].
abstract: OBJECTIVE
  To investigate the effects of ethyl pyruvate (EP) on cell-mediated immune function in rats with delayed resuscitation after burn injury, and its potential regulatory mechanism.
  
  
  METHODS
  Wistar rats were subjected to 30% full-thickness scald injury with delayed resuscitation. One hundred and three male rats were randomly divided into normal controls (n=7), sham scald group (n=32), scald group (n=32) in which 40 ml/kg normal saline was infused peritoneally 6 hours after scald, and EP treatment group (n=32) in which 40 mg/kg EP was injected peritoneally 6 hours after scald. Animals were sacrificed on postburn day 1, 3, 5, and 7, and spleen was collected to determine splenocyte proliferation, IL-2 production and cell-surface IL-2 receptor (IL-2R) expression.
  
  
  RESULTS
  Splenic lymphocyte proliferation responses to T cell mitogen, concanavalin A (Con A), were depressed from 1 to 7 days after scald injury (all P<0.05). Meanwhile, in comparison with sham scald group, burn injury resulted in a significant decrease in splenic production of IL-2 on postburn day 1, 3, as well as 5, and a marked suppression of IL-2R expression on days 1 and 3 postburn (all P<0.05). Treatment with EP after burn injury showed a dramatic restoration of lymphocyte proliferation rate and increased production of IL-2 at various time points (all P<0.05). However, treatment with EP did not affect IL-2R expression compared with scalded rats (all P>0.05).
  
  
  CONCLUSIONS
  Treatment with EP could markedly elevate splenic T lymphocyte proliferation response and increase production of IL-2 following burn injury, thereby improving cell-mediated immunity in thermally injured rats with delayed resuscitation.
authors.affiliation: Burns Institute, 304 th Clinical Department of General Hospital of PLA, Beijing 100037, China.
authors.forename: Yue-qing
authors.initials: YQ
authors.lastname: Dong
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Yao
authors.forename: Peng
authors.initials: P
authors.lastname: Wei
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Dong
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Sheng
mesh_terms: D000818:Animals; D002056:Burns / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation; D004195:Disease Models, Animal; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D011773:Pyruvates / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D011773:Pyruvates; D015375:Receptors, Interleukin-2; C046522:ethyl pyruvate