title: Escherichia coli small heat shock proteins IbpA/B enhance activity of enzymes sequestered in inclusion bodies.
abstract: Escherichia coli small heat shock proteins, IbpA/B, function as molecular chaperones and protect misfolded proteins against irreversible aggregation. IbpA/B are induced during overproduction of recombinant proteins and bind to inclusion bodies in E. coli cells. We investigated the effect of DeltaibpA/B mutation on formation of inclusion bodies and biological activity of enzymes sequestered in the aggregates in E. coli cells. Using three different recombinant proteins: Cro-beta-galactosidase, beta-lactamase and rat rHtrA1 we demonstrated that deletion of the ibpA/B operon did not affect the level of produced inclusion bodies. However, in aggregates containing IbpA/B a higher enzymatic activity was detected than in the IbpA/B-deficient inclusion bodies. These results confirm that IbpA/B protect misfolded proteins from inactivation in vivo.
authors.affiliation: Department of Biochemistry, University of Gdańsk, Gdańsk, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kuczyńska-Wiśnik
authors.forename: Dorota
authors.initials: D
authors.lastname: Zurawa-Janicka
authors.forename: Joanna
authors.initials: J
authors.lastname: Narkiewicz
authors.forename: Joanna
authors.initials: J
authors.lastname: Kwiatkowska
authors.forename: Barbara
authors.initials: B
authors.lastname: Lipińska
authors.forename: Ewa
authors.initials: E
authors.lastname: Laskowska
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D002479:Inclusion Bodies / Q000201:enzymology* / Q000378:metabolism; D009154:Mutation; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C097578:IbpA protein, E coli; C097579:IbpB protein, E coli; D011994:Recombinant Proteins