The level of some acute phase proteins, total protein, gamma-globulins and activity of lysozyme in blood plasma of rats supplemented with vitamin E and exposed to ozone.
In rats exposed for 28 days (5 hours a day) to ozone at a concentration of 0.5 ppm and receiving alpha-tocopherol at doses of 4.5 mg/rat and 15 mg/rat, levels of acute phase proteins (APP)--C-reactive protein (CRP), ceruloplasmin (Cp), total protein, gamma-globulins, and activity of lysozyme in blood serum were studied. The assays were performed in the presence of respective control groups, i.e. rats receiving the same doses of alpha-tocopherol but not exposed to ozone, a group of animals not supplemented with vitamin but exposed to ozone, a group of animals injected with physiological fluid and a control group not subjected to any of the treatments. The study revealed that the ozone-exposed animals had an increased lysozyme activity and a decreased total protein level. However, in rats protected by alpha-tocopherol and exposed to ozone, the concentration of APP, lysozyme activity and total protein were found to be decreased. Similar relationships also occurred in animals receiving alpha-tocopherol and not exposed to ozone.
Jakubowski
K
K
Department of Pathology and Pharmacology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland. jakar@uwm.edu.pl
Jedlińska-Krakowska
M
M
Siwicki
A K
AK
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001738:Biuret Reaction; D001798:Blood Proteins / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008297:Male; D009113:Muramidase / Q000097:blood*; D009391:Nephelometry and Turbidimetry / Q000662:veterinary; D010126:Ozone / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry / Q000662:veterinary; D014810:Vitamin E / Q000008:administration & dosage*; D005719:gamma-Globulins / Q000378:metabolism*
D000209:Acute-Phase Proteins; D000975:Antioxidants; D001798:Blood Proteins; D005719:gamma-Globulins; D014810:Vitamin E; D010126:Ozone; D009113:Muramidase