Simultaneous determination of morphine, oxycodone, morphine-3-glucuronide, and noroxycodone concentrations in rat serum by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry.
An assay using high performance liquid chromatography (HPLC)-electrospray ionization-tandem mass spectrometry (ESI-MS-MS) was developed for simultaneously determining concentrations of morphine, oxycodone, morphine-3-glucuronide, and noroxycodone, in 50 microl samples of rat serum. Deuterated (d(3)) analogues of each compound were used as internal standards. Samples were treated with acetonitrile to precipitate plasma proteins; acetonitrile was removed from the supernatant by centrifugal evaporation before analysis. Limits of quantitation (ng/ml) and their between-day accuracy and precision (%deviation and %CV) were--morphine, 3.8 (4.3% and 7.6%); morphine-3-glucuronide, 5.0 (4.5% and 2.9%); oxycodone, 4.5 (0.4% and 9.3%); noroxycodone, 5.0 (8.5% and 4.6%).
Edwards
Stephen R
SR
School of Pharmacy, The University of Queensland, St Lucia, Brisbane, Qld 4072, Australia.
Smith
Maree T
MT
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009019:Morphinans / Q000097:blood*; D009020:Morphine / Q000097:blood*; D009022:Morphine Derivatives / Q000097:blood*; D010098:Oxycodone / Q000097:blood*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D009019:Morphinans; D009022:Morphine Derivatives; D009020:Morphine; C077256:noroxycodone; D010098:Oxycodone; C018108:morphine-3-glucuronide