[Full-length sequence of hepatitis B virus isolated from high incidence hepatocellular carcinoma area-Longan county].
OBJECTIVE
To understand full-length sequence of HBV isolated from high incidence hepatocellular carcinoma area-Longan county, Guangxi.


METHODS
The nested polymerase chain reaction (nPCR) was used for amplifying the whole HBV DNA in sera of asymptomatic carriers. The products were sequenced by clone sequencing and homological analysis.


RESULTS
This isolate contained 3 215 bases. The genotype was C and the serotype was adw. There were 40 point mutations in polymerase gene which made 11 amino acids change. There were 11,2 and 3 point mutations in PreS1, PreS2 and S gene respectively which made 3,1,1, amino acids change. Six point mutations including the double mutations (nt 1762 A to T, 1764 G to A) were found in X gene leading to 4 amino acids change. There were 13 point mutations in C gene which made 2 amino acids change. No mutation was found in a determinant and Pre C. The isolate was quite close to the isolate from Vietnamese in evolution while far from the genotype C isolates from Shanghai, Beijing and Tibet.


CONCLUSIONS
No special sequence was found in the isolate from high incidence hepatocellular carcinoma area, Longan county, Guangxi.
Ge
Xian-min
XM
Guangxi Institute of Occupational Health, Nanning 530021, China.
Fang
Zhong-liao
ZL
Li
Dan-ya
DY
Zhao
Lian-san
LS
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology; D002353:Carrier State / Q000821:virology*; D002681:China / Q000453:epidemiology; D005838:Genotype; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008113:Liver Neoplasms / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D017385:Sequence Homology