title: Nonlymphoid peritoneal cells suppress the T cell response to Mls.
abstract: Comparative analyses of the ability of lymphoid tissue to present the minor lymphocyte stimulatory (Mls) superantigen Mls-1a in vitro revealed that all tissues containing mature B cells, except peritoneal cavity (PerC) cells, induced Mls-1a-specific T cell activation. Irradiation and mitomycin C treatment, addition of IL-2 and IL-12, and neutralization of IL-10 and TGF-beta did not restore Mls-1a antigen presentation by PerC cells. Co-culture studies revealed that PerC cells actively suppress the T cell response to Mls-1a. PerC cells from severe-combined immune-defective (SCID) mice also suppressed this response indicating that nonlymphoid cells mediate this effect. These results suggest that in addition to antigen processing and presentation, resident peritoneal cavity cells may temper lymphocyte activation.
authors.affiliation: Department of Biology, Rider University, Lawrenceville, NJ 08648-3099, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Rosini
authors.forename: Robin
authors.initials: R
authors.lastname: Matlack
authors.forename: Justin
authors.initials: J
authors.lastname: Taylor
authors.forename: Koko F
authors.initials: KF
authors.lastname: Howell
authors.forename: Kenneth
authors.initials: K
authors.lastname: Yeh
authors.forename: Anthony
authors.initials: A
authors.lastname: Pennello
authors.forename: James E
authors.initials: JE
authors.lastname: Riggs
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D017209:Apoptosis; D001402:B-Lymphocytes / Q000276:immunology; D002450:Cell Communication / Q000276:immunology; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology / Q000494:pharmacology / Q000502:physiology; D005260:Female; D007108:Immune Tolerance; D008213:Lymphocyte Activation / Q000276:immunology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology*; D016685:Mitomycin / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D016747:Minor Lymphocyte Stimulatory Antigens; D016685:Mitomycin