[Effects of arsenic trioxide on the subcellular localization of PML/PML-RARalpha protein in leukemic cells].
OBJECTIVE
In order to illustrate the possible roles of PML-RARalpha protein in arsenic trioxide (AsO3)-induced NB4 cell apoptosis.


METHODS
Effects of As2O3 on the subcellular localization of PML-RARalpha in NB4 cells were studied.


RESULTS
(1) Anti-PML serum staining was reduced and PML granules emerged in the perinuclear cytoplasm in a diffuse pattern in HL-60 cells under As2O3 treatment; (2) abnormal PML/PML-RARalpha granules were decreased; (3) NB4 cells accumulated anti-PML serum staining granules in the cytoplasms were increased and similar accumulation also found in apoptotic cells; and (4) pretreatment with all-trans retinoic acid (ATRA) for 24 or 48 hours did not alter the As2O3 effects.


CONCLUSIONS
As2O3-induced apoptosis was independent of the retinoic acid signal pathway, and it might be regulated by PML/PML-RARalpha and/or other related genes.
Ni
J
J
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University 200025.
Chen
G
G
Zhu
J
J
Zhong
H
H
Tang
W
W
Li
X
X
Xiong
S
S
Shen
Z
Z
Chen
S
S
Wang
Z
Z
Chen
L
L
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects* / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D009687:Nuclear Proteins / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*; D000071156:Promyelocytic Leukemia Protein; D021381:Protein Transport / Q000187:drug effects; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D000072482:Retinoic Acid Receptor alpha; D014157:Transcription Factors / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D000970:Antineoplastic Agents; D001152:Arsenicals; D009687:Nuclear Proteins; D010087:Oxides; D000071156:Promyelocytic Leukemia Protein; C000607071:RARA protein, human; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human; D014212:Tretinoin; D000077237:Arsenic Trioxide