The effect of O-glcnac glycosylation of rat liver nucleoproteins on their acute phase-dependent binding ability to the hormone responsive element of the haptoglobin gene.
We examined whether the transcriptional activation of the rat haptoglobin (Hp) gene during the acute phase (AP) response reflects the O-linked N-acetylglucosamine (O-GlcNAc) status of liver nucleoproteins (NPs) and their binding for the hormone responsive element (HRE). After deglycosylation with N-acetylglucosaminidase of the O-GlcNAc glycoproteins obtained by WGA, affinity chromatography and South-Western analysis, it was observed that only increased HRE binding ability of p64/p70 in control and p51 obtained from turpentine-treated rats can be directly attributed to the presence of O-GlcNAc residues. Therefore, expression of the rat Hp gene could be controlled by this modification of certain trans-acting NPs.
Grigorov
I
I
Department of Molecular Biology, Institute of Biological Research, Belgrade, Serbia and Montenegro. iligri@ibiss.bg.ac.yu
Cvetković
I
I
Milosavljević
T
T
Uskoković
A
A
Bogojević
D
D
Ivanović-Matić
S
S
Poznanović
G
G
Petrović
M
M
D000117:Acetylglucosamine / Q000235:genetics / Q000378:metabolism*; D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D006031:Glycosylation; D006242:Haptoglobins / Q000235:genetics* / Q000378:metabolism*; D006728:Hormones / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D020218:Response Elements / Q000187:drug effects / Q000235:genetics; D015533:Transcriptional Activation / Q000502:physiology
D000209:Acute-Phase Proteins; D006242:Haptoglobins; D006728:Hormones; D009698:Nucleoproteins; D000117:Acetylglucosamine