title: Synthesis and characterization of the diastereomers Lambda- and Delta-[Ru(bpy)2(m-bpy-L-Arg-Gly-L-Asn-L-Ala-L-His-L-Glu-L-Arg)]Cl2 1H NMR studies on their interactions with the deoxynucleotide duplex d[(5'-GCGCTTAAGCGC-3')2] and d[(5'-CGCGATCGCG-3')2].
abstract: The diastereomeric complexes Lambda- and Delta-[Ru(bpy)(2)(m-bpy-7p)]Cl(2), (bpy=2,2'-bipyridine, m-bpy-7p=4-methyl-4'-Arg-Gly-Asn-Ala-His-Glu-Arg-CONH(2)-2,2'-bipyridine) were synthesized and characterized and their binding properties to the deoxynucleotide duplexes d(5'-CGCGATCGCG-3')(2) and d(5'-GCGCTTAAGCGC-3')(2) were studied by means of (1)H NMR spectroscopy. 7p is part of the recognition loop of the restriction endonuclease MunI, a type II restriction enzyme from Mycoplasma unidentified which recognizes the palindromic hexanucleotide sequence C/AATTG and cleaves it as indicated by the slash. The Delta-isomer binds to the terminal CG/GC major groove of d(CGCGATCGCG)(2) decanucleotide, whereas the Lambda-isomer approaches the GCT/CGA sequence. On the other hand, weak binding of the Delta-isomer to the end of d(GCGCTTAAGCGC)(2) into two different orientations is observed. In the case of the Lambda-isomer, the bpy ligand(s) are located into the major groove of the central TT/AA sequence. The role of appended peptide sequences in sequence selectivity binding to DNA is being addressed.
authors.affiliation: Laboratory of Inorganic and General Chemistry, Department of Chemistry, University of Ioannnina, Ioannina 45110, Greece.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Myari
authors.forename: Nick
authors.initials: N
authors.lastname: Hadjiliadis
authors.forename: Achilleas
authors.initials: A
authors.lastname: Garoufis
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008667:Metalloproteins / Q000138:chemical synthesis* / Q000737:chemistry*; D015394:Molecular Structure; D009174:Mycoplasma / Q000201:enzymology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012428:Ruthenium; D013237:Stereoisomerism
chemical_list: D008667:Metalloproteins; D009838:Oligodeoxyribonucleotides; D012428:Ruthenium; C059776:endodeoxyribonuclease MfeI; D015252:Deoxyribonucleases, Type II Site-Specific