Promotion of U937 cell adhesion on polypropylene surfaces bearing phosphorylcholine functionalities.
Phosphorylcholine (PC) groups were grafted onto ammonia plasma-treated biaxially-oriented polypropylene (BOPP) surfaces, via (a) reductive amination of phosphorylcholine glyceraldehyde and (b) a two-step procedure involving the chemical amplification of surface amine groups with tris(2-aminoethyl amine) and subsequent reductive amination of phosphorylcholine glyceraldehyde. The occurrence of grafting was ascertained by X-ray photoelectron spectroscopy (XPS) and attenuated total reflectance Fourier-transform infrared (ATR-FT-IR) spectroscopy. The wettability of PC-modified surfaces was assessed by dynamic contact-angle measurements using the Wilhelmy plate method. Human U937 macrophages adhered and proliferated to a significantly larger extent on PC-modified surfaces, compared to unmodified or ammonia plasma-modified BOPP.
Gong
Yong-Kuan
YK
Department of Chemistry and Faculty of Pharmacy, University of Montreal, Montréal, Québec, Canada H3C 3J7.
Mwale
Fackson
F
Wertheimer
Michael R
MR
Winnik
Francoise M
FM
D000641:Ammonia / Q000737:chemistry; D002448:Cell Adhesion; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D015394:Molecular Structure; D010767:Phosphorylcholine / Q000032:analysis* / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry* / Q000378:metabolism*; D013057:Spectrum Analysis; D020298:U937 Cells
D011126:Polypropylenes; D010767:Phosphorylcholine; D000641:Ammonia