title: Biological weighting function of the UV-B-induced impairment of phototaxis in the freshwater ciliate Ophryoglena flava.
abstract: We determined the biological weighting function (BWF) of the effect of UV radiation on phototaxis of the freshwater, histophagous ciliate Ophryoglena flava. Dose-effect curves were measured by exposing the cells to 12 different irradiation regimens obtained with two different levels of UV-B radiation and by using six filters with cutoff wavelengths ranging from 280 to 335 nm. The results show that there are significant damages to phototaxis at the doses used and that the effect increases when the cutoff is shifted toward short wavelengths. The data were used to calculate the BWF of phototaxis impairment by applying a nonlinear fit procedure. The BWF thus obtained decays exponentially with increasing wavelength in agreement with similar findings reported in the literature for other systems.
authors.affiliation: Institute of Biophysics CNR, Research Area of Pisa, via G. Moruzzi 1, Pisa, Italy. roberto.marangoni@pi.ibf.cnr.it
authors.forename: Roberto
authors.initials: R
authors.lastname: Marangoni
authors.forename: Fabio
authors.initials: F
authors.lastname: Marroni
authors.forename: Domenico
authors.initials: D
authors.lastname: Gioffré
authors.forename: Francesco
authors.initials: F
authors.lastname: Ghetti
authors.forename: Giuliano
authors.initials: G
authors.lastname: Colombetti
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000528:radiation effects; D016798:Ciliophora / Q000502:physiology* / Q000528:radiation effects*; D005618:Fresh Water; D009068:Movement / Q000502:physiology / Q000528:radiation effects*; D014466:Ultraviolet Rays