In vivo labeling and turnover of prolyl hydroxylase and a related immunoreactive protein.
Prolyl hydroxylase and an immunologically related protein (CRP) were purified from neonatal rabbit skin at various time periods following administration of 3H-leucine. The peak incorporation of label into prolyl hydroxylase was found to be 12 hours, while peak incorporation into CRP occurred within 2 hours. Semi-log plots of the loss of radioactivity from these protein pools against time indicated an apparent T 1/2 for prolyl hydroxylase of 78 hours, and a T 1/2 of CRP of 44 hours. Calculated Kd values indicate that that breakdown of active enzyme does not account for the amount of CRP found in tissues.
Chichester
C O
CO
Department of Pharmacology and Toxicology, University of Rhode Island, Kingston, Rhode Island 02881, USA.
Fuller
G C
GC
Cardinale
G J
GJ
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D007930:Leucine / Q000378:metabolism; D011393:Procollagen-Proline Dioxygenase / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012867:Skin / Q000201:enzymology; D013997:Time Factors; D014316:Tritium / Q000737:chemistry
D000906:Antibodies; D000941:Antigens; D011506:Proteins; D014316:Tritium; D011393:Procollagen-Proline Dioxygenase; D007930:Leucine