Design of a multiplex PCR for genotyping 16 short tandem repeats in degraded DNA samples.
The molecular genotyping of individuals and reconstruction of kinship through short and high polymorphic DNA markers, so-called short tandem repeats (STR), has become an important and efficient method in anthropology and forensic science. The here introduced experimental design describes a multiplex PCR capable of simultaneously amplifying 16 STRs and the sex determinant locus amelogenin in a short fragment lengths range from 84 bp to 275 bp. Thus, the design depends predominantly on the routines for DNA typing of historical samples with highly degraded ancient DNA. It is shown, that the newly designed multiplex PCR is suitable for successful typing of both forensic and historical material.
Schilz
Felix
F
Historical Anthropology and Human Ecology, Institute of Zoology and Anthropology, University of Göttingen. fschilz@gwdg.de
Hummel
Susanne
S
Herrmann
Bernd
B
D000483:Alleles; D053523:Amelogenin; D000883:Anthropology / Q000379:methods; D002048:Burial; D002874:Chromosome Mapping; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods; D003746:Dental Enamel Proteins / Q000235:genetics*; D005060:Europe; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D049690:History, Ancient; D006801:Humans; D010164:Paleopathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D019849:Sex Determination Processes; D020080:Tandem Repeat Sequences / Q000235:genetics*
D053523:Amelogenin; D003746:Dental Enamel Proteins; D005819:Genetic Markers