title: DNA-directed in vitro synthesis of elongation factor Tu.
abstract: The in vitro synthesis of EF-Tu was studied using the transducing phage lambdarifd18 DNA as template. The EF-Tu synthesized was identified by its immunological properties, gel analysis, and its ability to interact with GDP and EF-Ts. Two other unidentified polypeptides were also precipitated from the incubation mixture by EF-Tu antiserum. The synthesis of total EF-Tu immunoprecipitable material was depressed about one-half by guanosine-5'-diphosphate-3'-diphosphate which was shown to inhibit the transcription process.
authors.affiliation: Department of Biochemistry, Roche Institute of Molecular Biology, Nutley, New Jersey 07110, USA.
authors.forename: F
authors.initials: F
authors.lastname: Chu
authors.forename: D L
authors.initials: DL
authors.lastname: Miller
authors.forename: T
authors.initials: T
authors.lastname: Schulz
authors.forename: H
authors.initials: H
authors.lastname: Weissbach
authors.forename: N
authors.initials: N
authors.lastname: Brot
mesh_terms: D000906:Antibodies / Q000276:immunology; D002474:Cell-Free System; D002850:Chromatography, Gel; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006153:Guanosine Diphosphate / Q000378:metabolism; D006159:Guanosine Tetraphosphate / Q000494:pharmacology; D047468:Immunoprecipitation; D007700:Kinetics; D010444:Peptide Elongation Factor Tu / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000187:drug effects; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000906:Antibodies; D004269:DNA, Bacterial; D010455:Peptides; D006153:Guanosine Diphosphate; D006159:Guanosine Tetraphosphate; D010444:Peptide Elongation Factor Tu