title: In situ observation of molecular diffusion in solid supports using two-photon fluorescence microscopy.
abstract: We demonstrate that two-photon fluorescence microscopy is a useful tool for monitoring the diffusion of molecules through polymeric solid supports. As a proof of principle, TentaGel beads were bound to a cover slip, and diffusion of Rhodamine 6G through single beads was observed in real time. Diffusion experiments performed in 40 different beads indicate that there is a considerable degree of heterogeneity in diffusion rates from bead to bead.
authors.affiliation: Eugene F. Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, USA.
authors.forename: Mariko M
authors.initials: MM
authors.lastname: Taniguchi
authors.forename: Richard A
authors.initials: RA
authors.lastname: Farrer
authors.forename: John T
authors.initials: JT
authors.lastname: Fourkas
mesh_terms: D003116:Color; D004058:Diffusion; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008863:Microspheres; D012235:Rhodamines / Q000032:analysis
chemical_list: D012235:Rhodamines; C026188:rhodamine 6G