title: Phosphatidylinositol-4,5-bisphosphate: actin dynamics and the regulation of ATP-dependent and -independent secretion.
abstract: It has long been believed that the cortical actin cytoskeleton plays an important role in regulating the secretion of hormones and neurotransmitters. In this study, we investigated the control of actin dynamics in primary neuroendocrine cells and determined the relationship of actin dynamics to various components of the secretory response. The amount of cortical f-actin in chromaffin cells was quantified in confocal images of cells stained with Alexa Fluor 568 phalloidin. Manipulations that decreased levels of phosphatidylinositol-4,5-bisphosphate (PIP(2)) (e.g., removal of ATP, the expression of a protein that can sequester PIP(2)) rapidly reduced the amount of cortical actin. In contrast, cytoskeletal disruptors such as latrunculin were much less able to reduce cortical actin levels, indicating that the amount of cortical f-actin depends more strongly on PIP(2) than on the availability of g-actin. Not only does PIP(2) regulate actin, but actin regulates the level of PIP(2), as revealed by PIP(2) labeling studies. Manipulation of cortical actin had differing effects on the ATP-dependent and -independent components of secretion. ATP-dependent secretion was particularly sensitive to changes in cortical actin stability and was inhibited by expression of a protein (Yersinia pestis protein kinase A) that disassembles cortical f-actin and by pharmacological agents that promote either disassembly or stabilization of actin. The data suggest that an ATP-dependent component of secretion requires rapid changes in actin dynamics. These results point to a complex web of interactions involving PIP(2), actin, and the secretory response.
authors.affiliation: Department of Pharmacology, University of Michigan Medical School, 1301 MSRB III, Ann Arbor, MI 48109-0632, USA. mbittner@umich.edu
authors.forename: Mary A
authors.initials: MA
authors.lastname: Bittner
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Holz
mesh_terms: D000199:Actins / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000378:metabolism; D003599:Cytoskeleton / Q000502:physiology; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000032:analysis / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D010738:Type C Phospholipases / Q000737:chemistry
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D019269:Phosphatidylinositol 4,5-Diphosphate; D013844:Thiazoles; D053778:Thiazolidines; D019382:Human Growth Hormone; D000255:Adenosine Triphosphate; C079893:ypkA protein, Yersinia; D017346:Protein Serine-Threonine Kinases; D010738:Type C Phospholipases; C037068:latrunculin B