title: Effects of repeated injection of local anesthetic on sciatic nerve blocks response.
abstract: In order to examine whether repeated sciatic nerve blocks showed tachyphylaxis and continuity of sciatic nerve with spinal cord affected development of tachyphylaxis when assayed in vivo by duration of depression compound action potentials (CAP), rats were anesthetized with halothane, ventilated, monitored and supported with stable hemodynamics and temperature. Posterior tibial nerve distally and sciatic nerve in thigh were exposed, placed on bipolar silver electrodes for stimulation and recording respectively. Three sequential sciatic nerve blocks were performed between these electrodes using 0.15 ml of 3% chloroprocaine. Nine rats were chosen to observe the effects of repeated sciatic nerve blocks on CAP. In another 18 rats, a second investigator exposed the sciatic nerve near its origin at spinal cord and randomly performed nerve cut and sham (n=9), and closed the incision blinding the electrophysiologic investigator. The results showed that electrical stimulated tibial nerve induced sciatic nerve Aalpha/beta, Adelta, C fiber mediated CAP waves. CAP amplitudes were remained stable during whole experimental procedure. CAP amplitudes were decreased completely with 3% chloroprocaine blocked sciatic nerve and recovered fully. The duration of CAP depression were reduced with repeated blocks. There were no selective blocked effects on Aalpha/beta, Adelta, C fiber mediated CAP. With sciatic nerve cut proximally, there was no statistical significant tachyphylaxis with 3% chloroprocaine repeated blocked sciatic nerve, and the duration of first and third blocked Adelta fiber mediated CAP was 108+/-20 and 92+/-14 min respectively (P>0.05). In normal rats the duration of first and third blocked Adelta fiber mediated CAP was 110+/-20 and 75+/-16 min respectively (P<0.05). It was suggested that tachyphylaxis to local anesthetics can occur in rats repeated blocked sciatic nerve when assayed in vivo by duration of depression CAP. The continuity of sciatic nerve with spinal cord is one of the important factors affecting the development of tachyphylaxis.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Shantou University Medical College, Shantou 515041 , China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Huaiping
authors.initials: H
authors.lastname: Liu
authors.forename: R T
authors.initials: RT
authors.lastname: Wilder
authors.forename: C B
authors.initials: CB
authors.lastname: Berde
mesh_terms: D000779:Anesthetics, Local / Q000008:administration & dosage*; D000818:Animals; D009407:Nerve Block; D011343:Procaine / Q000008:administration & dosage / Q000031:analogs & derivatives; D051381:Rats; D012584:Sciatic Nerve; D013618:Tachyphylaxis / Q000502:physiology
chemical_list: D000779:Anesthetics, Local; D011343:Procaine; C004616:chloroprocaine