Analgesic activity of di-caffeoylquinic acids from roots of Lychnophora ericoides (Arnica da serra).
The Brazilian medicinal plant Lychnophora ericoides Mart. (Asteraceae) has been used in traditional medicine to treat wounds, pain and inflammation. As part of our continuing investigation of this commercial phytomedicine, we focused on the polar fractions of the plant, since it is employed as alcoholic and hydroalcoholic preparations. The analgesic bioguided fractionation of the root polar extract yielded 3,5-di-O-[E]-caffeoylquinic acid, 4,5-di-O-[E]-caffeoylquinic acid and 3,4,5-tri-O-[E]-caffeoylquinic acid. The n-butanol fraction and the di-caffeoylquinic acids showed significant analgesic activity in the acetic acid-induced mouse writhing test at low but not at high doses. These findings support, at least in part, the validity of the use of Lychnophora ericoides roots in traditional medicine.
dos Santos
Michel David
MD
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do café s/n degree, CEP 14040-903, Ribeirão Preto, São Paulo, SP, Brazil.
Gobbo-Neto
Leonardo
L
Albarella
Laura
L
de Souza
Glória Emília Petto
GE
Lopes
Norberto Peporine
NP
D019342:Acetic Acid; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000627:therapeutic use*; D018650:Arnica; D004305:Dose-Response Relationship, Drug; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009005:Monosaccharides / Q000302:isolation & purification / Q000627:therapeutic use*; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000627:therapeutic use*
C500584:3,4,5-tri-O-(E)-caffeoylquinic acid; C500585:3,5-di-O-(E)-caffeoylquinic acid; C500583:4,5-di-O-(E)-caffeoylquinic acid; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009005:Monosaccharides; D010936:Plant Extracts; D011801:Quinic Acid; D019342:Acetic Acid