title: Eukaryotic arylamine N-acetyltransferase. Investigation of substrate specificity by high-throughput screening.
abstract: Arylamine N-acetyltransferases (NAT; EC 2.3.1.5) catalyse the transfer of acetyl groups from acetylCoA to xenobiotics, including drugs and carcinogens. The enzyme is found extensively in both eukaryotes and prokaryotes, yet the endogenous roles of NATs are still unclear. In order to study the properties of eukaryotic NATs, high-throughput substrate and inhibitor screens have been developed using pure soluble recombinant Syrian hamster NAT2 (shNAT2) protein. The assay can be used with a wide range of compounds and was used to determine substrate specificity of shNAT2. We describe the expression and characterisation of shNAT2 and also purified recombinant human NAT1 and NAT2, including the use of the assay to explore the substrate specificities of each of the enzymes. Hamster NAT2 has similar substrate specificity to human NAT1, acetylating para-aminobenzoate but not arylhydrazine and hydralazine compounds. The overlapping but distinct substrate-specific activity profiles of human NAT1 and NAT2 were clearly observed from the screen. Naturally occurring compounds were tested as substrates or inhibitors of shNAT2 and succinylCoA was found to be a potent inhibitor of shNAT2.
authors.affiliation: Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK. akane.kawamura@pharm.ox.ac.uk
authors.forename: Akane
authors.initials: A
authors.lastname: Kawamura
authors.forename: James
authors.initials: J
authors.lastname: Graham
authors.forename: Adeel
authors.initials: A
authors.lastname: Mushtaq
authors.forename: Stefanos A
authors.initials: SA
authors.lastname: Tsiftsoglou
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Vath
authors.forename: Patrick E
authors.initials: PE
authors.lastname: Hanna
authors.forename: Carston R
authors.initials: CR
authors.lastname: Wagner
authors.forename: Edith
authors.initials: E
authors.lastname: Sim
mesh_terms: D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D006224:Cricetinae; D005057:Eukaryotic Cells / Q000737:chemistry / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008027:Light; D011817:Rabbits; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D012542:Scattering, Radiation; D013379:Substrate Specificity / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; D001191:Arylamine N-Acetyltransferase