Determination of free radical scavenging activity of quercetin, rutin, luteolin and apigenin in H2O2-treated human ML cells K562.
We investigated protective effects of four flavonoids against H2O2- induced DNA damage in human myelogenous leukemia cells (K562) using the comet assay. The structural difference of studied flavonoids -- quercetin, rutin, luteolin and apigenin -- are characterized by the number of hydroxyl groups on the B ring. The presence of an o-dihydroxy structure on the B-ring confers a higher degree of stability to the flavonoid phenoxyl radicals by participating in electron delocalization and is, therefore, an important determinant for antioxidative potential. The results correlate with earlier published data obtained in murine leukemia cell line L1210. Hydrogen peroxide induced in human K562 cells a concentration-dependent increase of single cell DNA strand breaks. The strongest inhibition against H2O2-induced DNA damage (44%, 42%) was found in a range of luteolin and quercetin concentrations of 20-100 micromol/l. Protective effect of rutin (100 and 1000 micromol/l) was only marginal (8-10%). Apigenin had no protective effect on DNA single strand breaks induced by H2O2. Luteolin and quercetin are therefore effective in the protection of human single cell DNA from oxidative attack.
Horváthová
K
K
Department of Experimental Therapy of Tumors, Cancer Research Institute, Slovak Academy of Sciences, 833 91 Bratislava, Slovak Republic. katarina.horvathova@savba.sk
Novotný
L
L
Tóthová
D
D
Vachálková
A
A
D047310:Apigenin / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020014:K562 Cells / Q000187:drug effects*; D047311:Luteolin / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D012431:Rutin / Q000494:pharmacology*
D016166:Free Radical Scavengers; D012431:Rutin; D047310:Apigenin; D011794:Quercetin; D006861:Hydrogen Peroxide; D047311:Luteolin