Simultaneous estimation of a Y-specific fragment, an X-specific fragment and sex determination of forensic studies in real-time PCR.
The human sex test in forensic multiplexes is based on the amelogenin gene on both the X and Y chromosomes commonly used in sex genotyping. In this work sex determination across the quantification of nuclear DNA was achieved by real-time PCR of a segment of the X-Y homologous amelogenin (AMG) gene that allowed the simultaneous estimation of a Y- and an X-specific fragment. The AMG real-time PCR design has been used to quantify a set of forensic casework samples.
Roccazzello
A M
AM
Istituto Ricerca Medica e Ambientale, Via Paolo Vasta 158/c, 95024 Acireale (CT), Italia. roccazzello@irma-srl.com
Tringali
G
G
Barbaro
A
A
Cormaci
P
P
Barbaro
A
A
Insirello
E
E
D053523:Amelogenin; D016022:Case-Control Studies; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D019849:Sex Determination Processes; D014083:Tooth Germ
C507199:AMELX protein, human; D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA