In situ observation of molecular diffusion in solid supports using two-photon fluorescence microscopy.
We demonstrate that two-photon fluorescence microscopy is a useful tool for monitoring the diffusion of molecules through polymeric solid supports. As a proof of principle, TentaGel beads were bound to a cover slip, and diffusion of Rhodamine 6G through single beads was observed in real time. Diffusion experiments performed in 40 different beads indicate that there is a considerable degree of heterogeneity in diffusion rates from bead to bead.
Taniguchi
Mariko M
MM
Eugene F. Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, USA.
Farrer
Richard A
RA
Fourkas
John T
JT
D003116:Color; D004058:Diffusion; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008863:Microspheres; D012235:Rhodamines / Q000032:analysis
D012235:Rhodamines; C026188:rhodamine 6G