Changes in amino acids and lipids during embryogenesis of European lobster, Homarus gammarus (Crustacea: Decapoda).
We studied the amino acid and lipid dynamics during embryogenesis of Homarus gammarus. Major essential amino acids (EAA) in the last stage of embryonic development were arginine, lysine and leucine; major nonessential amino acids (NEAA) were glutamic acid, aspartic acid, valine and glycine. The highest percent of utilization occurred in respect to EAA (27.8%), mainly due to a significant decrease (p<0.05) of methionine (38.3%) and threonine (36.0%). NEAA also decreased significantly (p<0.05, 11.4%), namely serine (38.1%), tyrosine (26.4%) and glutamic acid (25.7%). In contrast, the free amino acid content increased significantly (p<0.05) during embryonic development, especially the free nonessential amino acids (FNEAA). In the last stage, the most abundant FNEAA were glycine, proline, alanine and taurine, and the major free essential amino acids (FEAA) were arginine, lysine and leucine. Lipid content decreased significantly (p<0.05) during embryonic development. A substantial decrease in all neutral lipid classes was observed (>80% of utilization). Major fatty acids were 16:0, 18:0, 18:1n-9, 18:2n-6, 18:3n-3, 20:5n-3 and 22:6n-3. Unsaturated (UFA) and saturated fatty acids (SFA) were used up at similar rates (76.5% and 76.3%, respectively). Within UFA, monounsaturates (MUFA) were consumed more than polyunsaturates (PUFA) (82.9% and 67.5%, respectively).
Rosa
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Departamento de Inovação Tecnológica e Valorização dos Produtos da Pesca, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal. rrosa@ipimar.pt
Calado
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Andrade
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Narciso
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Nunes
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D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D047108:Embryonic Development; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005260:Female; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000145:classification; D008121:Nephropidae / Q000196:embryology* / Q000378:metabolism*; D010063:Ovum / Q000737:chemistry / Q000254:growth & development; D014867:Water / Q000032:analysis
D000596:Amino Acids; D005227:Fatty Acids; D008055:Lipids; D014867:Water