title: A design of fluorescent probes for superoxide based on a nonredox mechanism.
abstract: Fluorometric detection of O2-* is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward O2-* over H2O2, t-BuOOH, NaOCl, 1O2, HO*, NO*, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2-* released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan. h-maeda@phs.osaka-u.ac.jp
authors.forename: Hatsuo
authors.initials: H
authors.lastname: Maeda
authors.forename: Kayoko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nomura
authors.forename: Iho
authors.initials: I
authors.lastname: Kohno
authors.forename: Leila
authors.initials: L
authors.lastname: Hafsi
authors.forename: Noritsugu
authors.initials: N
authors.lastname: Ueda
authors.forename: Shoko
authors.initials: S
authors.lastname: Yoshida
authors.forename: Masako
authors.initials: M
authors.lastname: Fukuda
authors.forename: Yuka
authors.initials: Y
authors.lastname: Fukuyasu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Norio
authors.initials: N
authors.lastname: Itoh
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D015195:Drug Design; D005452:Fluoresceins / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005470:Fluorometry; D005978:Glutathione / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D019271:Hypoxanthine / Q000737:chemistry; D009249:NADP / Q000737:chemistry; D010084:Oxidation-Reduction; D013451:Sulfonic Acids / Q000737:chemistry; D013482:Superoxide Dismutase / Q000737:chemistry; D013481:Superoxides / Q000032:analysis / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000737:chemistry
chemical_list: D001557:Benzenesulfonates; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013451:Sulfonic Acids; D013481:Superoxides; D019271:Hypoxanthine; C001073:2,4-dinitrobenzenesulfonic acid; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D005978:Glutathione