Lipid bilayer tethered inside a nanoporous support: a solid-state nuclear magnetic resonance investigation.
(31)P and (1)H solid-state nuclear magnetic resonance (NMR) experiments have been designed with the aim of studying directly the formation of supported bilayers tethered inside nanoporous aluminum oxide supports as a model of biomimetic membranes. The static and magic angle spinning (31)P NMR spectra of the supported bilayers have been compared with the experimental and simulated spectra of a simpler model with cylindrical geometry, namely a phospholipid bilayer adsorbed on an oriented polymer sheet. The broadening observed for the nanoporous model is most likely due to the presence of paramagnetic ions in the aluminum oxide. A phospholipid lateral diffusion coefficient of (2.8 +/- 0.4) x 10(-8) cm(2)/s has been measured for the tethered bilayer on a spherical support, indicating a good fluidity as compared with adsorbed membrane models.
Wattraint
Olivier
O
Unité de Génie Enzymatique et Cellulaire, UMR 6022 du CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France.
Arnold
Alexandre
A
Auger
Michèle
M
Bourdillon
Christian
C
Sarazin
Catherine
C
D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D016062:Porosity
D008051:Lipid Bilayers; D010743:Phospholipids