title: [Generation and identification of rice T-DNA insertional mutant lines].
abstract: By using the matured embryos of Japonica rice variety Zhonghua No. 11 as explants, rice transformation was performed by Agrobacterium-mediated co-cultivation method, resulting in 1489 independent transgenic rice plants that carry a T-DNA insertion. Genomic DNA gel-blot and PCR analyses showed that 69.8% of the total lines contain the inserted T-DNA. The flanking sequence of T-DNA in transgenic rice plants was analyzed using Tail-PCR. In addition, we have evaluated 1066 T1 transgenic lines on heading days, plant height and panicles per hill, and found different types of mutants from a number of lines.
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China.
authors.forename: Shuang-Yong
authors.initials: SY
authors.lastname: Yan
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Zhi
authors.forename: Xin-Jie
authors.initials: XJ
authors.lastname: Liu
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhang
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Tan
authors.forename: Shi-Gui
authors.initials: SG
authors.lastname: Li
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA