title: Benzamil, a blocker of epithelial Na(+) channel-induced upregulation of artery oxygen pressure level in acute lung injury rabbit ventilated with high frequency oscillation.
abstract: The epithelial Na(+) transport via an epithelial Na(+) channel (ENaC) expressed in the lung epithelium would play a key role in recovery from lung edema at acute lung injury by removing the fluid in lung luminal space. The lung edema causes dysfunction of gas exchange, decreasing oxygen pressure level of artery [P(aO(2))]. To study if ENaC plays a key role in recovering P(aO(2)) from a decreased level to a normal one in acute lung injury, we applied benzamil (20microM, a specific blocker of ENaC) to the lung luminal space in acute lung injury treated with high frequency oscillation ventilation (HFOV) that is a lung-protective ventilation with a lower tidal volume and a smaller pressure swing than conventional mechanical ventilation (CMV). Benzamil facilitated the recovery of P(aO(2)) in acutely injured lung with HFOV but not CMV. The observation suggests that in acutely injured lung treated with HFOV an ENaC blocker, benzamil, can be applied as a therapeutic drug for acute lung injury combing with HFOV.
authors.affiliation: Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Taguchi
authors.forename: Naomi
authors.initials: N
authors.lastname: Niisato
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Sawabe
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Yasutomi
authors.initials: Y
authors.lastname: Hirai
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Marunaka
mesh_terms: D000584:Amiloride / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D006612:High-Frequency Ventilation / Q000379:methods; D010100:Oxygen / Q000378:metabolism*; D011175:Positive-Pressure Respiration / Q000379:methods; D011817:Rabbits; D012128:Respiratory Distress Syndrome / Q000378:metabolism*; D026941:Sodium Channel Blockers / Q000494:pharmacology*; D015222:Sodium Channels / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D026941:Sodium Channel Blockers; D015222:Sodium Channels; C013407:benzamil; D000584:Amiloride; D010100:Oxygen