Interaction between a nanovirus-like component and the Tobacco curly shoot virus/satellite complex.
The biological role of DNA1, a nanovirus-like component shown to be associated with the begomovirus/satellite complex, has not yet been identified. Here, we demonstrated that DNA1 of Tobacco curly shoot virus isolate Y35 (TbCSV-Y35) attenuated leaf-curling symptoms induced by TbCSV-Y35 or TbCSV-Y35 plus Y35 DNAbeta in the early stage of symptom development and induced leaf cluster at a later stage of symptom development in Nicotiana benthamiana plants. The leaf disc assay demonstrated that TbCSV-Y35 DNA1 replicated autonomously. Southern blot analysis revealed that TbCSV-Y35 DNA1 reduced viral DNA accumulation. Viral DNA accumulation was not reduced when plants were co-inoculated with TbCSV-Y35 DNAbeta, but the TbCSV-Y35 DNAbeta level was dramatically reduced in the presence of TbCSV-Y35 DNA1. To determine whether the interaction between TbCSV/satellite complex and DNA1 had isolate specificity, DNA1 of TbCSV isolate Y132 was cloned and sequenced. It was found to have 75% nucleotide sequence identity with TbCSV-Y35 DNA1. Infectivity tests showed that TbCSV-Y132 DNA1 had no effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAbeta in N. benthamiana plants, although Y132 DNA1 could replicate in these plants.
Wu
Pei-Jun
PJ
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
Zhou
Xue-Ping
XP
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004279:DNA, Viral; D017791:Geminiviridae / Q000235:genetics*; D017343:Genes, Plant; D025682:Nanovirus / Q000235:genetics*; D010942:Plant Viruses; D013997:Time Factors; D014026:Nicotiana / Q000378:metabolism / Q000821:virology*
D004279:DNA, Viral; D004247:DNA