title: Synthetic surfaces for ribonuclease adsorption.
abstract: Intact RNA and DNA are of central importance to biochemical research and biotechnology. The preservation of these nucleic acids requires the absence of nuclease activity. Here, radical-mediated polymerization of vinylsulfonate on resin and glass surfaces is shown to produce a high-density poly(vinylsulfonate) coating that sequesters ribonucleases from aqueous solutions quickly and completely. The adsorptive efficacy of this coating exceeds that of other known coatings by > or =10(7)-fold. Surfaces coated with poly(vinylsulfonate) could be used to maintain the integrity of ribonucleic acids in a variety of contexts.
authors.affiliation: Department of Biochemistry and Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Bryan D
authors.initials: BD
authors.lastname: Smith
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Soellner
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Raines
mesh_terms: D000327:Adsorption; D006801:Humans; D012260:Ribonucleases / Q000737:chemistry*
chemical_list: D012260:Ribonucleases