[Effects of total alkaloids of Tripterygium hypoglaucum Hutch on tk gene of mouse lymphoma cells].
OBJECTIVE
To investigate the effects of total alkaloids of Tripterygium hypoglaucum Hutch (THH) on the tk gene of mouse lymphoma cells.


METHODS
L5178Y cells were infected with total alkaloids of THH with different concentrations and put into single-cell wells at different time phases. Then the numbers of positive wells were counted and the cell plating efficiency, relative suspension growth and mutation frequency were determined.


RESULTS
Total alkaloids of THH (0.1-2.0 g x L(-1)) induced tk locus mutation with mutation frequency 2-9 times higher than that of spontaneous mutation frequency of L5178Y cells. There were two different phenotypes of mutation colonies, large colony and small colony, but the main colony was large colony. This phenomenon might be related with the mutagenesis of THH.


CONCLUSIONS
Total alkaloids of THH can exert toxicity and mutagenic effects on tk gene in L5178Y cells, and there may be range limit in gene mutation.
Liu
Sheng-xue
SX
Department of Health Toxicology, Third Military Medical University, Chongqing 400038, China.
Cao
Jia
J
Yuan
Jian
J
Huang
Ping
P
Shua
Pei-qiang
PQ
Honma
Masamitsu
M
D000470:Alkaloids / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007940:Leukemia L5178 / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D051379:Mice; D009153:Mutagens / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry; D017354:Point Mutation; D013937:Thymidine Kinase / Q000235:genetics*; D029757:Tripterygium / Q000737:chemistry
D000470:Alkaloids; D009153:Mutagens; D013937:Thymidine Kinase