[GM-CSF regulates the Vp16 induced leukemic cell apoptosis].
OBJECTIVE
To explore the regulatory effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on the apoptosis of leukemic cells induced by Vp16.


METHODS
High expression retrovirus vector, N2A/CMV/GM-CSF, was constructed and transferred into human leukemic cell line HL-60, and Vp16 (final concentration 10microg/ml) was administered to transferred or nontransferred HL-60 cells.


RESULTS
After Vp16 treatment, characteristic changes for apoptosis emerged in HL-60 cells. However, these findings did not emerge in GM-CSF gene transferred HL-60 cells.


CONCLUSIONS
GM-CSF can suppress the leukemic cell apoptosis induced by Vp16.
Li
X
X
Institute of Hematology, Union Hospital, Tongji Medical University, Wuhan 430022.
Li
C
C
Zou
P
P
Chen
Q
Q
Chen
Y
Y
Wang
B
B
Zhang
Z
Z
Hou
Y
Y
D017209:Apoptosis / Q000187:drug effects*; D005047:Etoposide / Q000494:pharmacology*; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*
D005047:Etoposide; D016178:Granulocyte-Macrophage Colony-Stimulating Factor