title: [Cloning and sequencing of Sry gene of muntiacus].
abstract: Using the primers from SRY gene--HMG Box for PCR amplification in genomic DNA of Muntiacus reevesi cell strains, a 220bp fragment was obtained in the male but not in the female. The 220bp fragment was cloned into the pGEM-T vector using T/A clone method. The identified positive clone was sequenced. The result shows that 82.6% nucleotides(152bp/184bp) are homologous between Muntiacus Sry and human SRY gene. It suggests that SRY is highly conserved during evolution.
authors.affiliation: Genetic Research Center, Medical College of Southeast University, Nanjing 210009, China. luxiaoxuan@hotmail.com
authors.forename: Xiao-Xuan
authors.initials: XX
authors.lastname: Lu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiang-Nian
authors.initials: XN
authors.lastname: Shan