The influence of oxidative stress on permeability of capillary vessels in the cheek pouch of hamsters with alloxan-induced diabetes.
BACKGROUND
The aim of the study was to assess the influence of oxidative stress on the increase of permeability of capillary vessels in animals with alloxan-induced diabetes.


METHODS
The studies were performed in microcirculation system of hamster cheek pouch. After the blockade of histamine receptors and administration of diamine oxidase (DAO) and histamine into circulation fluorescein angiography was done. In addition, the influence of superoxide dismutase, aminoguanidine (DAO inhibitor) and trascolan (protease inhibitor) on vascular permeability caused by superoxide radical generation in DAO/histamine system was assessed.


RESULTS
The number of extravasal leakages in the group receiving HA and DAO was significantly higher (p < 0.001) than in the groups receiving potential vascular "sealers", e.g. SOD, aminoguanidine or trascolan. In the group receiving aminoguanidine the number of leakages was significantly lower (p < 0.05) compared to the group receiving SOD or trascolan.


CONCLUSIONS
The protective effect of aminoguanidine, superoxide dismutase or trascolan decreasing the vascular permeability, suggests that the increased vascular permeability is a result of superoxide radical generation by diamine oxidase.
Ciechanowski
K
K
Department of Nephrology, Pomeranian Medical University, Szczecin, Poland. kazcie@sci.pam.szczecin.pl
Kedzierska
K
K
Gołembiewska
E
E
Mikłaszewicz
A
A
Domański
L
L
Bober
J
J
Dutkiewicz
G
G
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002196:Capillaries / Q000187:drug effects* / Q000503:physiopathology*; D002199:Capillary Permeability / Q000187:drug effects*; D002610:Cheek / Q000098:blood supply* / Q000503:physiopathology*; D006224:Cricetinae; D018384:Oxidative Stress / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D006631:Amine Oxidase (Copper-Containing)