title: Anti-CXCR4 monoclonal antibodies recognizing overlapping epitopes differ significantly in their ability to inhibit entry of human immunodeficiency virus type 1.
abstract: CXCR4 is one of two physiologically relevant human immunodeficiency type 1 (HIV-1) entry coreceptors. Studies of CXCR4 mutants have not clearly identified the determinants of coreceptor function and specificity. We therefore used a panel of monoclonal antibodies to further elucidate CXCR4 expression, structure, and function. Our findings show the existence of conformational subpopulations of CXCR4 that are in equilibrium on the cell surface but are not cell type specific as previously reported. HIV-1 X4 isolates can interact with multiple CXCR4 conformations in order to gain entry into target cells.
authors.affiliation: Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, USA.
authors.forename: Xavier
authors.initials: X
authors.lastname: Carnec
authors.forename: Lan
authors.initials: L
authors.lastname: Quan
authors.forename: William C
authors.initials: WC
authors.lastname: Olson
authors.forename: Uriel
authors.initials: U
authors.lastname: Hazan
authors.forename: Tatjana
authors.initials: T
authors.lastname: Dragic
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D018604:Epitope Mapping; D015497:HIV-1 / Q000378:metabolism / Q000472:pathogenicity*; D006367:HeLa Cells; D006801:Humans; D017354:Point Mutation; D011487:Protein Conformation; D019718:Receptors, CXCR4 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes
chemical_list: D000911:Antibodies, Monoclonal; D019718:Receptors, CXCR4