Establishment of stable hFis1 knockdown cells with an siRNA expression vector.
Yeast Fis1p participates in mitochondrial fission, together with Dnm1p and Mdv1p. Recently, human Fis1 (hFis1) was reported to be involved in mitochondrial fission, together with Drp1. We established stable transformants with an hFis1 siRNA expression vector. In the stable hFis1 knockdown cells, hFis1 expression was suppressed to approximately 10%, and mitochondrial fission, induced by cisplatin treatment, was delayed. In addition, mouse Fis1 (mFis1) expression promoted mitochondrial fission and cell death in the hFis1 knockdown cells, suggesting that mFis1 complements the function of hFis1. These hFis1 siRNA expression vectors may be useful for studying the molecular function of mammalian Fis1.
Arai
Ryoichi
R
Protein Research Group and Genome Exploration Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Ito
Kaori
K
Wakiyama
Motoaki
M
Matsumoto
Eiko
E
Sakamoto
Ayako
A
Etou
Yuuka
Y
Otsuki
Makiko
M
Inoue
Mio
M
Hayashizaki
Yoshihide
Y
Miyagishi
Makoto
M
Taira
Kazunari
K
Shirouzu
Mikako
M
Yokoyama
Shigeyuki
S
D000818:Animals; D015153:Blotting, Western; D002945:Cisplatin / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D005816:Genetic Complementation Test; D005821:Genetic Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D013997:Time Factors
D018076:DNA, Complementary; C476525:FIS1 protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D034741:RNA, Small Interfering; D002945:Cisplatin