title: A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer.
abstract: Prostate cancer is among the most common tumors in industrialized nations. However, little is known about the molecular events underlying its development. In the present study we used suppressive subtraction hybridization (SSH) in combination with laser-assisted microdissection in order to compare gene expression between prostate carcinoma and the normal prostate proper. Both are mixed tissues which consist of an epithelial and a stromal compartment. We first compared mRNA (cDNA) expression by SSH and then used real-time quantitative RT-PCR analysis of microdissected tissue probes in order to verify differential expression of subtracted cDNA clones. We also used differentially expressed cDNAs for the synthesis of radiolabelled riboprobes in order to attribute differential expression to specific cell types in tissue sections by in situ hybridization. Using this approach we found an up-regulation of ubiquitin carboxyl extension protein 1 (UBCEP-1) mRNA in prostate carcinoma cells compared to the normal glandular epithelium of the prostate proper. UBCEP-1 mediated ubiquitin chain elongation may promote prostate carcinoma development by increasing via the proteasome pathway the degradation of proteins which are involved in growth inhibition or apoptosis.
authors.affiliation: Medizinische Poliklinik, University of Bonn, D-53111 Bonn, Germany. yonko@uni-bonn.de
authors.forename: Yon
authors.initials: Y
authors.lastname: Ko
authors.forename: Tanja
authors.initials: T
authors.lastname: Hahn
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Zhan Lu
authors.initials: ZL
authors.lastname: Ma
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Marcus
authors.initials: M
authors.lastname: Rothe
authors.forename: Alexandra
authors.initials: A
authors.lastname: Florin
authors.forename: Stefan
authors.initials: S
authors.lastname: Fronhoffs
authors.forename: Peter
authors.initials: P
authors.lastname: Albers
authors.forename: Agapios
authors.initials: A
authors.lastname: Sachinidis
authors.forename: Hans
authors.initials: H
authors.lastname: Vetter
authors.forename: Annette
authors.initials: A
authors.lastname: Kaminski
authors.forename: Peter
authors.initials: P
authors.lastname: Behrens
authors.forename: Axel
authors.initials: A
authors.lastname: Wellmann
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wernert
mesh_terms: D000368:Aged; D017209:Apoptosis; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D003720:Densitometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D007834:Lasers; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D018075:RNA, Complementary / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics*; D013050:Spectrometry, Fluorescence; D035181:TATA-Box Binding Protein / Q000378:metabolism; D013997:Time Factors; D014452:Ubiquitins / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018075:RNA, Complementary; D012333:RNA, Messenger; C408062:RPS27a protein, human; D012269:Ribosomal Proteins; D035181:TATA-Box Binding Protein; D014452:Ubiquitins