Evolution of dorsal-ventral axis formation in arthropod appendages: H15 and optomotor-blind/bifid-type T-box genes in the millipede Glomeris marginata (Myriapoda: Diplopoda).
In Drosophila, the T-box genes optomotor-blind (omb) and H15 have been implicated in specifying the development of the dorso-ventral (DV) axis of the appendages. Results from the spider Cupiennius salei have suggested that this DV patterning system may be at least partially conserved. Here we extend the study of the DV patterning genes omb and H15 to a representative of the Myriapoda in order to add to the existing comparative data set and to gain further insight into the evolution of the DV patterning system in arthropod appendages. The omb gene of the millipede Glomeris marginata is expressed on the dorsal side of all appendages including trunk legs, maxillae, mandibles, and antennae. This is similar to what is known from Drosophila and Cupiennius and suggests that the role of omb in instructing dorsal fates is conserved in arthropods. Interestingly, the lobe-shaped portions of the mouthparts do not express omb, indicating that these are ventral components and thus may be homologous to the endites present in the corresponding appendages in insects. Concerning the H15 gene we were able to identify two paralogous genes in Glomeris. Both genes are expressed in the sensory organs of the maxilla and antenna, but only Gm-H15-1 is expressed along the ventral side of the trunk legs. The expression is more extensive than in Cupiennius, but less so than in Drosophila. In addition, no ventral expression domain is present in the maxilla, mandible, and antenna. Because of this, the role of H15 in the determination of ventral fate remains unclear.
Prpic
Nikola-Michael
NM
Department for Evolutionary Genetics, Institute for Genetics, University of Cologne, Weyertal 121, 50931 Köln, Germany.
Janssen
Ralf
R
Damen
Wim G M
WG
Tautz
Diethard
D
D000818:Animals; D001181:Arthropods; D019521:Body Patterning; D019070:Cell Lineage; D003001:Cloning, Molecular; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism / Q000502:physiology*; D019143:Evolution, Molecular; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007313:Insecta; D009419:Nerve Tissue Proteins / Q000378:metabolism / Q000502:physiology*; D010802:Phylogeny; D020825:T-Box Domain Proteins / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
D029721:Drosophila Proteins; C495572:H15 protein, Drosophila; D009419:Nerve Tissue Proteins; D020825:T-Box Domain Proteins; C072962:bi protein, Drosophila