Transcriptomic fingerprinting of bone marrow-derived hepatic beta2m-/Thy-1+ stem cells.
The aim of the present study was to determine if the bone marrow (BM) beta2m-/Thy-1+ stem cells isolated from common bile duct ligated (CBDL) rats possess hepatocyte-like characteristics in their global gene expression profiles. The Affymetrix RG U34A arrays were used to conduct transcriptomic profiling on BM beta2m-/Thy-1+ stem cells isolated from CBDL and control rats as well as primary hepatocytes. Forty-one probe sets were up-regulated more than 2-fold in CBDL-derived beta2m-/Thy-1+ BM stem cells compared to control BM stem cells. Twenty-seven probe sets were present in both CBDL-derived beta2m-/Thy-1+ BM stem cells and control hepatocytes but absent in control beta2m-/Thy-1+ BM stem cells, including Tcf1 and Dbp. Compared to the control beta2m-/Thy-1+ BM stem cells, CBDL-derived beta2m-/Thy-1+ BM stem cells shared more commonly expressed genes with hepatocytes. Overall, CBDL-derived beta2m-/Thy-1+ stem cells displayed a different transcriptomic fingerprint compared with beta2m-/Thy-1+ BM stem cells isolated from control rats; and CBDL-derived beta2m-/Thy-1+ stem cells started to express some hepatocyte-like genes.
Wang
Charles
C
Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA. charles.wang@cshs.org
Chelly
Marjorie R
MR
Chai
NingNing
N
Tan
Yongxi
Y
Hui
Thomas
T
Li
Hongmei
H
Farkas
Daniel L
DL
Demetriou
Achilles A
AA
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D003135:Common Bile Duct / Q000378:metabolism; D016172:DNA Fingerprinting; D020869:Gene Expression Profiling; D023281:Genomics; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D015854:Up-Regulation; D001613:beta 2-Microglobulin / Q000032:analysis
D012333:RNA, Messenger; D018800:Thy-1 Antigens; D001613:beta 2-Microglobulin