title: Rearrangement of simian virus 40 regulatory region is not required for induction of progressive multifocal leukoencephalopathy in immunosuppressed rhesus monkeys.
abstract: Rearrangements of the JC virus (JCV) regulatory region (RR) are consistently found in the brains of patients with progressive multifocal leukoencephalopathy (PML), whereas the archetype RR is present in their kidneys. In addition, the C terminus of the large T antigen (T-Ag) shows greater variability in PML than does the rest of the coding region. To determine whether similar changes in simian virus 40 (SV40) are necessary for disease induction in monkeys, we sequenced the SV40 RR and the C terminus of the T-Ag from the brain of simian/human immunodeficiency virus (SHIV)-infected monkey 18429, which presented spontaneously with an SV40-associated PML-like disease, as well as from the peripheral blood mononuclear cells (PBMC), kidneys, and brains of SV40-seronegative, SHIV-infected monkeys 21289 and 21306, which were inoculated with the 18429 brain SV40 isolate. These animals developed both SV40-associated PML and meningoencephalitis. Thirteen types of SV40 RR were characterized. Compared to the SV40 archetype, we identified RRs with variable deletions in either the origin of replication, the 21-bp repeat elements, or the late promoter, as well as deletions or duplications of the 72-bp enhancer. The archetype was the most prominent RR in the brain of monkey 18429. Shortly after inoculation, a wide range of RRs could be found in the PBMC of monkeys 21289 and 21306. However, the archetype RR became the predominant type in their blood, kidneys, and brains at the time of sacrifice. On the contrary, the T-Ag C termini remained identical in all compartments of the three animals. These results indicate that unlike JCV in humans, rearrangements of SV40 RR are not required for brain disease induction in immunosuppressed monkeys.
authors.affiliation: Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, RE-213, 330 Brookline Ave., Boston, MA 02215, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Dang
authors.forename: Michael K
authors.initials: MK
authors.lastname: Axthelm
authors.forename: Norman L
authors.initials: NL
authors.lastname: Letvin
authors.forename: Igor J
authors.initials: IJ
authors.lastname: Koralnik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000737:chemistry / Q000235:genetics; D002478:Cells, Cultured; D005347:Fibroblasts / Q000821:virology; D006678:HIV / Q000472:pathogenicity; D015658:HIV Infections / Q000150:complications*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000503:physiopathology* / Q000821:virology; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D016415:Sequence Alignment; D017384:Sequence Deletion; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications*; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity; D013539:Simian virus 40 / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D000957:Antigens, Viral, Tumor