title: Quantitative determination of urinary 8-hydroxy-2'-deoxyguanosine level in healthy Japanese volunteers.
abstract: 8-hydroxy-2'-deoxyguanosine (8-OHdG), as a measure of oxidative stress, was measured in healthy Japanese volunteers using an ELISA (New 8-OHdG Check, JICA). Analysis of daytime spot urine of 83 healthy male subjects and smoking habit, exercise and age revealed significant correlation only between the urinary level of 8-OHdG and age. As the inter-individual variation of 8-OHdG of the daytime spot urine was relatively high, we next determined inter-and intra-individual variation of 5 healthy volunteers. The levels of 8-OHdG/creatinine in morning spot urine significantly correlated with 8-OHdG levels in 24-h pool urine. Thus, a morning spot urine sample can be used for the measurement of 8-OHdG instead of inconvenient 24-h sampling.
authors.affiliation: National Food Research Institute, Tsukuba 305-8642, Japan. m3miwa@nodai.ac.jp
authors.forename: Misao
authors.initials: M
authors.lastname: Miwa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsumaru
authors.forename: Yukari
authors.initials: Y
authors.lastname: Akimoto
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Naito
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Ochi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000375:Aging / Q000502:physiology; D015415:Biomarkers / Q000652:urine; D003404:Creatinine / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D007564:Japan; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress; D012016:Reference Values; D012044:Regression Analysis
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine