title: Differential expression of histochemical characteristics in the developing olfactory receptor cells in a flatfish, barfin flounder (Verasper moseri).
abstract: Differentiation of the histochemical characteristics of the olfactory receptor cells (ORC) was examined by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and calretinin (CR) and lectin histochemistry for Phaseolus vulgaris agglutinin-L (PHA-L) in the developing olfactory epithelium (OE) of the barfin flounder. PGP 9.5 immunoreactivity was diffuse and CR immunoreactivity was restricted at day 7, but these immunoreactivities became intense in the OE toward day 91. Crypt cells were first identified at day 56. PHA-L staining was faint at day 28, but became intense toward day 91. These findings suggest that PGP 9.5-immunopositive cells, CR-immunopositive cells, crypt cells and PHA-L-reactive cells differentiate independently in the developing OE and constitute subsets of the ORC in the OE.
authors.affiliation: Laboratory of Veterinary Anatomy, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Mori
authors.forename: Shouichiro
authors.initials: S
authors.lastname: Saito
authors.forename: Masafumi
authors.initials: M
authors.lastname: Amano
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamanome
authors.forename: Kazumi
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Oikawa
authors.forename: Kunio
authors.initials: K
authors.lastname: Yamamori
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D064032:Calbindin 2; D002454:Cell Differentiation / Q000502:physiology*; D005432:Flounder / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D009831:Olfactory Mucosa / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D064032:Calbindin 2; D010835:Phytohemagglutinins; D010940:Plant Proteins; D064030:S100 Calcium Binding Protein G; C101135:phytohemagglutinin L protein, Phaseolus vulgaris; D043222:Ubiquitin Thiolesterase