[Breeding transgenic plants with safe or no selective markers].
The bio-safety of selective markers in transgenic plants has been a hot spot in the field of plant genetic engineering. To solve the problem of selective markers in the transgenic plants, two means of producing transgenic plants have been developed. One is the utilization of bio-safe positive selective markers which are genes mainly related to metabolism of auxins and carbohydrates. The other is the establishment of transformation systems allowing marker genes to be eliminated from the transgenic plants, which include co-ransformation, double T-DNA border vectors, site-specific recombination and transposition. All these approaches of plant genetic engineering will benefit breeding transgenic plants with bio-safety.
Li
Xiao-Bing
XB
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. xbli@genetics.ac.cn
Chen
Cai-Yan
CY
Zhai
Wen-Xue
WX