Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.
Exposure of mice to a 120 dB SPL broad band click sound for 3 h per day induced expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in the organ of Corti, lateral wall tissue and spiral ganglion cells. In the organ of Corti, HIF-1alpha was expressed in inner and outer hair cells, pillar cells and Deiters' cells, but not in Hensen's cells or the tectorial, Reissner's or basement membrane. HIF-1alpha expression was definitely increased in mice with a permanent threshold shift, where the hearing level did not recover, even after 4 weeks. These results suggest that noise may induce tissue hypoxia in the inner ear and that the further study on the role of HIF-1alpha will be needed.
Chung
Jong Woo
JW
Department of Otolaryngology, Asan Medical Center, University of Ulsan, College of Medicine, 388-1 Pungnap-Dong, Songpa-Gu, Seoul, 138-736, Korea. jwchung@amc.seoul.kr
Kang
Hun Hee
HH
Shin
Jeong Eun
JE
Kim
Joung Uk
JU
D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D001309:Auditory Threshold / Q000502:physiology; D015153:Blotting, Western / Q000379:methods; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D007758:Ear, Inner / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009622:Noise; D009687:Nuclear Proteins / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; C497443:Hif1a protein, mouse; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D014157:Transcription Factors