Biotin deficiency reduces expression of SLC19A3, a potential biotin transporter, in leukocytes from human blood.
In evaluating potential indicators of biotin status, we quantitated the expression of biotin-related genes in leukocytes from human blood of normal subjects before and after inducing marginal biotin deficiency. Biotin deficiency was induced experimentally by feeding an egg-white diet for 28 d. Gene expression was quantitated for the following biotin-related proteins: methylcrotonyl-CoA carboxylase chains A (MCCA) and B (MCCB); propionyl-CoA carboxylase chains A (PCCA) and B (PCCB); pyruvate carboxylase (PC); acetyl-CoA carboxylase isoforms A (ACCA) and B (ACCB); holocarboxylase synthetase (HCS); biotinidase; and 2 potential biotin transporters: sodium-dependent multivitamin transporter (SMVT) and solute carrier family 19 member 3 (SLC19A3). For 7 subjects who successfully completed the study, the abundance of the specific mRNAs was determined by quantitative real-time RT-PCR at d 0 and 28. At d 28, SLC19A3 expression had decreased to 33% of d 0 (P < 0.02 by two-tailed, paired t test). Expression of MCCA, PCCA, PC, ACCA, ACCB, HCS, biotinidase, and SMVT decreased to approximately 80% of d 0 (P < 0.05). Expression of the MCCB and PCCB chains that do not carry the biotin-binding motif did not change significantly; we speculate that expression of the biotin-binding chains of biotin-dependent carboxylases is more responsive to biotin status changes. These data provide evidence that expression of SLC19A3 is a relatively sensitive indicator of marginal biotin deficiency.
Vlasova
Tatyana I
TI
Biochemistry & Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Stratton
Shawna L
SL
Wells
Amanda M
AM
Mock
Nell I
NI
Mock
Donald M
DM
D000328:Adult; D001483:Base Sequence; D001710:Biotin / Q000172:deficiency*; D017931:DNA Primers; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D026901:Membrane Transport Proteins / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D026901:Membrane Transport Proteins; C492291:SLC19A3 protein, human; D001710:Biotin