Identification of bifunctional GA and AG intrastrand crosslinks formed between cisplatin and DNA.
A combination of enzymatic digestion and electrospray ionisation mass spectrometry (ESI-MS) was used to characterise bifunctional adducts in which cisplatin is bound to GA base sequences in 8mer and 16mer oligonucleotides that do not contain other, higher affinity binding sites. The extent of formation of bifunctional adducts with GA base sequences was significant, but less than that seen with similar oligonucleotides containing either AG or GG sequences.
Gupta
Rajesh
R
Department of Chemistry, University of Wollongong, Northfields Avenue, Wollongong, NSW 2522, Australia.
Beck
Jennifer L
JL
Sheil
Margaret M
MM
Ralph
Stephen F
SF
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D002945:Cisplatin / Q000737:chemistry*; D003432:Cross-Linking Reagents; D018736:DNA Adducts / Q000737:chemistry*; D005092:Exonucleases; D066298:In Vitro Techniques; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D043264:Phosphodiesterase I; D021241:Spectrometry, Mass, Electrospray Ionization
D003432:Cross-Linking Reagents; D018736:DNA Adducts; D009838:Oligodeoxyribonucleotides; C057270:cisplatin-DNA adduct; D005092:Exonucleases; C022650:spleen exonuclease; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom; D002945:Cisplatin