title: [Comparison of denatured and non-denatured PAGE-silver-staining detection of PCR product of microsatellites].
abstract: The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels.
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing 100 094, China.
authors.forename: Lu-Jiang
authors.initials: LJ
authors.lastname: Qu
authors.forename: Xian-Yao
authors.initials: XY
authors.lastname: Li
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Du
authors.forename: Long-Chao
authors.initials: LC
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
mesh_terms: D000818:Animals; D002645:Chickens / Q000145:classification / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016622:Silver Staining
chemical_list: D004247:DNA