Comparison of three homogeneous methods for measuring high-density lipoprotein cholesterol with a precipitation method in diabetic and non-diabetic subjects.
Three homogenous methods for the automated measurement of high-density lipoprotein-cholesterol (HDL-C) are studied and compared with a precipitation method in diabetic and non-diabetic subjects. All three methods meet the precision criteria of the National Cholesterol Education Programme (NCEP). Triglycerides did not cause significant interference up to 10 mmol/L in the immuno-inhibition method (Wako) and up to 40 mmol/L in the Randox and Roche methods. Haemoglobin, up to a concentration of 5 g/L, had only negligible effect on the performance of all three homogeneous methods. Bilirubin caused an increasing positive bias in all methods above a concentration of 50 micromol/L. In comparison with the precipitation method, the new homogeneous methods agreed for type 1 diabetic patients but showed a positive bias for the control subjects and patients with type 2 diabetes. The bias of HDL-C levels in type 2 diabetes may be sufficient to affect the calculation of cardiovascular risk, and may therefore influence the decision to prescribe lipid-lowering medication.
Saeed
B O
BO
Department of Biochemistry, Queen Elizabeth Hospital, Gateshead, UK. saeedbakri@hotmail.com
Keeka
J
J
Smart
P
P
D001663:Bilirubin / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods; D011232:Chemical Precipitation; D008076:Cholesterol, HDL / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000097:blood
D008076:Cholesterol, HDL; D006454:Hemoglobins; D014280:Triglycerides; D001663:Bilirubin