title: Chromatographic detection of residual cellular DNA on short monolithic columns.
abstract: Analysis of crude samples from biotechnological processes is often required to demonstrate that residual host cell impurities are reduced or eliminated during purification. Current knowledge suggests that a continuous-cell-line DNA can be considered a cellular contaminant rather than a significant risk factor requiring removal to extremely low levels. Anion-exchange chromatography is one of the most important methods used in the downstream processing and analysis of different biomolecules. In this article, an application using Convective Interaction Media monolithic columns to improve the detection of residual cellular DNA is described.
authors.affiliation: Institute of Immunology Inc., Rockefeller Street 10, 10000 Zagreb, Croatia. dforcic@imz.hr
authors.forename: Dubravko
authors.initials: D
authors.lastname: Forcic
authors.forename: Karmen Branovic
authors.initials: KB
authors.lastname: Cakanic
authors.forename: Jelena
authors.initials: J
authors.lastname: Ivancic
authors.forename: Renata
authors.initials: R
authors.lastname: Jug
authors.forename: Milos
authors.initials: M
authors.lastname: Barut
authors.forename: Ales
authors.initials: A
authors.lastname: Strancar
authors.forename: Renata
authors.initials: R
authors.lastname: Mazuran
mesh_terms: D002460:Cell Line; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D015894:Genome, Human; D006801:Humans; D008094:Lithium / Q000737:chemistry; D008459:Measles virus / Q000737:chemistry
chemical_list: D004247:DNA; D008094:Lithium