Pro-oxidant action of phloxine B on fission yeast Schizosaccharomyces pombe.
A Schizosaccharomyces pombe mutant deficient in Cu,Zn-superoxide dismutase (sod1 mutant) was hypersensitive to phloxine B, which is used as a food-colouring agent and also to distinguish diploid strains of Sz. pombe from haploid strains, under illumination with light. The pro-oxidant nature of phloxine B was confirmed biochemically. The carbonyl content of proteins (which represents protein oxidation) increased, and the reduced form of glutathione was transiently decreased by phloxine B treatment under illumination with light. When cells were treated with phloxine B under light, carbonyl content of proteins in the sod1 mutant was greater than that in the wild-type and amount of glutathione was much decreased in the sod1 mutant compared with the wild-type. Genes induced by oxidative stress were induced by phloxine B under illumination with light and some were induced by phloxine B without light.
Mutoh
Norihiro
N
Department of Genetics, Institute for Developmental Research, Aichi Human Service Center, Kagiya-cho, Kasugai, Aichi 480-0392, Japan. normutoh@inst-hsc.jp
Kawabata
Masao
M
Nakagawa
Chiaki W
CW
Kitajima
Satoko
S
D015152:Blotting, Northern; D010697:Eosine I Bluish / Q000494:pharmacology*; D005505:Food Coloring Agents / Q000494:pharmacology*; D005816:Genetic Complementation Test; D005978:Glutathione / Q000378:metabolism; D008027:Light; D009154:Mutation; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000172:deficiency* / Q000378:metabolism
D005505:Food Coloring Agents; D016877:Oxidants; D012331:RNA, Fungal; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D005978:Glutathione; D010697:Eosine I Bluish