title: [Proteomic analysis of the salt tolerance mutant of wheat under salt stress].
abstract: Two dimensional electrophoresis was used to analyse the proteome of the salt-tolerant mutant of wheat (RH8706-49) and the salt-sensitive mutant of wheat (H8706-34) which had been treated by 1% NaCl for 72 hours. After being analysed by MALDI-TOF-MS and Mascot software, the qualitative and quantitative differences were identified between the two materials for five candidate proteins: H+-transporting two-sector ATPase, glutamine synthetase 2 precursor, putative 33 kD oxygen evolving protein of photosystem II and ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit. These five proteins are all belong to chloroplast proteins. They are likely to play a crucial role in keeping the function of the chloroplast and the whole cells when the plant was under salt-stress.
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
authors.forename: Chen-Min
authors.initials: CM
authors.lastname: Huo
authors.forename: Bao-Cun
authors.initials: BC
authors.lastname: Zhao
authors.forename: Rong-Chao
authors.initials: RC
authors.lastname: Ge
authors.forename: Yin-Zhu
authors.initials: YZ
authors.lastname: Shen
authors.forename: Zhan-Jing
authors.initials: ZJ
authors.lastname: Huang
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005974:Glutamate-Ammonia Ligase / Q000032:analysis; D009154:Mutation; D045332:Photosystem II Protein Complex / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics; D006180:Proton-Translocating ATPases / Q000032:analysis; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis; D012965:Sodium Chloride / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000235:genetics*
chemical_list: D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012965:Sodium Chloride; D006180:Proton-Translocating ATPases; D012273:Ribulose-Bisphosphate Carboxylase; C083818:glutamine synthetase 2; D005974:Glutamate-Ammonia Ligase