Intraocular levels of methotrexate after oral low-dose treatment in chronic uveitis.
OBJECTIVE
To determine the intraocular levels of methotrexate in low-dose treatment of noninfectious uveitis.


METHODS
One day after oral administration, the methotrexate level was measured in the aqueous humor and serum of a patient with noninfectious uveitis, who underwent cataract surgery. A fluorescence polarization immunoassay was used for determination.


RESULTS
After oral administration, methotrexate was only measurable in aqueous humor but not in serum.


CONCLUSIONS
In uveitis, orally administered low-dose methotrexate reaches detectable levels in aqueous humor, even in the absence of detectable levels in serum.
Puchta
Joachim
J
Department of Ophthalmology, Johann Wolfgang Goethe University, Frankfurt/Main, Germany. j.puchta@gmx.de
Hattenbach
Lars-Olof
LO
Baatz
Holger
H
D000284:Administration, Oral; D000293:Adolescent; D001082:Aqueous Humor / Q000378:metabolism*; D001682:Biological Availability; D002908:Chronic Disease; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008727:Methotrexate / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014605:Uveitis / Q000188:drug therapy* / Q000378:metabolism*
D007166:Immunosuppressive Agents; D008727:Methotrexate