Precise epitope mapping of malaria parasite inhibitory antibodies by TROSY NMR cross-saturation.
We have applied NMR cross-saturation with TROSY detection to the problem of precisely mapping conformational epitopes on complete protein antigen molecules. We have investigated complexes of the Fab fragments of two antibodies that have parasite inhibitory activity, bound to the important malaria vaccine candidate antigen, Plasmodium falciparum MSP1(19). The results indicate remarkable overlap between these epitopes for inhibitory antibodies, and will provide a basis for theoretical modeling of the antibody-antigen interface.
Morgan
William D
WD
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom. wmorgan@nimr.mrc.ac.uk
Frenkiel
Thomas A
TA
Lock
Matthew J
MJ
Grainger
Munira
M
Holder
Anthony A
AA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism; D000913:Antibodies, Protozoan / Q000737:chemistry* / Q000378:metabolism; D000962:Antimalarials / Q000737:chemistry* / Q000378:metabolism; D001666:Binding Sites, Antibody; D018604:Epitope Mapping / Q000379:methods; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000378:metabolism; D020066:Merozoite Surface Protein 1 / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*; D011487:Protein Conformation; D012680:Sensitivity and Specificity; D012996:Solutions; D020349:Surface Plasmon Resonance / Q000379:methods
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000962:Antimalarials; D016056:Immunodominant Epitopes; D007140:Immunoglobulin Fab Fragments; D020066:Merozoite Surface Protein 1; D012996:Solutions