Degradation of acyl-homoserine lactone molecules by Acinetobacter sp. strain C1010.
A bacterium C1010, isolated from the rhizospheres of cucumbers in fields in Korea, degraded the microbial quorum-sensing molecules, hexanoyl homoserine lactone (HHSL), and octadecanoyl homoserine lactone (OHSL). Morphological characteristics and 16S rRNA sequence analysis identified C1010 as Acinetobacter sp. strain C1010. This strain was able to degrade the acyl-homoserine lactones (AHLs) produced by the biocontrol bacterium, Pseudomonas chlororaphis O6, and a phytopathogenic bacterium, Burkholderia glumae. Co-cultivation studies showed that the inactivation of AHLs by C1010 inhibited production of phenazines by P. chlororaphis O6. In virulence tests, the C1010 strain attenuated soft rot symptom caused by Erwinia carotovora ssp. carotovora. We suggest Acinetobacter sp. strain C1010 could be a useful bacterium to manipulate biological functions that are regulated by AHLs in various Gram-negative bacteria.
Kang
Beom Ryong
BR
Agricultural Plant Research Center and Institute of Agriculture Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, South Korea.
Lee
Jung Hoon
JH
Ko
Sug Ju
SJ
Lee
Yong Hwan
YH
Cha
Jae Soon
JS
Cho
Baik Ho
BH
Kim
Young Cheol
YC
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism*; D000150:Acinetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018553:Cucumis sativus / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D012275:Oryza / Q000382:microbiology; D016973:Pectobacterium carotovorum / Q000254:growth & development / Q000472:pathogenicity; D010572:Pest Control, Biological; D010619:Phenazines / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D011549:Pseudomonas / Q000254:growth & development; D012988:Soil Microbiology
D010619:Phenazines; C088386:homoserine lactone; D015107:4-Butyrolactone