title: Insertional polymorphism of a non-LTR mobile element (NLRCth1) in European populations of Chironomus riparius (Diptera, Chironomidae) as detected by transposon insertion display.
abstract: The midge Chironomus riparius is distributed all over the Palearctic region and is well characterized both at the morphological and cytogenetic levels. Here we describe a population study based on the insertional polymorphism of the retroposon NLRCth1, by means of a S-SAP (sequence-specific amplification polymorphism) derived technique (transposon insertion display; TID). While a previous study of allozyme polymorphism in Russian samples showed little variability, all the amplicons we identified are polymorphic. Genetic distances between 6 natural populations were calculated according to Nei and did not show a positive correlation with geographic distances. The genetic diversity detected among individuals of a given population was one order of magnitude higher than that among populations. However, the value of phi(ST) was significant (p < 0.001) and indicates that natural populations are more genetically differentiated than random samples of individuals.
authors.affiliation: Department of Animal and Human Biology, University of Turin, via Accademia Albertina, 13, 10123 Torino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Zampicinini
authors.forename: A
authors.initials: A
authors.lastname: Blinov
authors.forename: P
authors.initials: P
authors.lastname: Cervella
authors.forename: V
authors.initials: V
authors.lastname: Guryev
authors.forename: G
authors.initials: G
authors.lastname: Sella
mesh_terms: D000483:Alleles; D000818:Animals; D002031:Bulgaria; D002683:Chironomidae / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004251:DNA Transposable Elements; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D007091:Image Processing, Computer-Assisted; D007558:Italy; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012426:Russia; D020079:Terminal Repeat Sequences
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D005819:Genetic Markers