Measurement of isoflavones using liquid chromatography with multi-channel coulometric electrochemical detection.
We evaluated the use of HPLC multi-channel coulometric electrochemical detection (HPLC-ECD) for the routine measurement of phytochemicals by analyzing standards and urine samples. Compounds were separated chromatographically and electrochemically with high sensitivity (10 ng/ml of isoflavones in urine). HPLC-ECD will enable better investigation into the relationship between lifestyle and the effects of phytochemicals on humans.
Ouchi
Kazuyuki
K
Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-0054, Japan.
Gamache
Paul
P
Acworth
Ian
I
Watanabe
Shaw
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D060754:Equol; D019833:Genistein / Q000032:analysis*; D007202:Indicators and Reagents; D007529:Isoflavones / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D007202:Indicators and Reagents; D007529:Isoflavones; D060754:Equol; C004742:daidzein; D019833:Genistein