Rapid quantitation of immunoglobulin G antibodies specific for blood group antigens A and B by surface plasmon resonance.
BACKGROUND
The measurement of immunoglobulin (Ig) G blood group A/B antibody(anti-A/B) levels is important for ABO-unmatched organ recipients because the effective removal of the antibodies improves their prognosis. Currently existing methods to detect IgG anti-A/B suffer limitations owing to high costs, low throughput, and poor adaptability to automation.


METHODS
We have developed a rapid means to quantitate IgG anti-A/B by surface plasmon resonance (SPR). To investigate the accuracy, a serially diluted plasma sample from a donor was measured with the SPR method. Moreover, IgG anti-A/B titers in 45 healthy volunteers were measured both by the SPR and by the standard tube test (TT) method, as were plasma samples from two ABO-unmatched organ recipients.


RESULTS
The change in titers when the same plasma was diluted was precisely reflected by the SPR method. The coefficients of correlation between SPR and TT methods for IgG anti-A and anti-B were 0.85 and 0.56, respectively. The SPR values also paralleled the TT values, which showed a decline in titers after the removal of antibodies by double-filtration plasmapheresis or plasma exchange.


CONCLUSIONS
This SPR method can be used to measure IgG anti-A/B titers in the plasma very quickly and quantitatively.
Kimura
Shinya
S
Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kawahara-cho Shogoin, Sakyo-ku, Kyoto, Japan.
Yurugi
Kimiko
K
Segawa
Hidekazu
H
Kuroda
Junya
J
Sato
Kiyoshi
K
Nogawa
Masaki
M
Yuasa
Takeshi
T
Egawa
Hiroto
H
Tanaka
Koichi
K
Maekawa
Taira
T
D000017:ABO Blood-Group System / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D020349:Surface Plasmon Resonance
D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007075:Immunoglobulin M