title: Molecular cloning and characterization of a novel cold-regulated gene from Capsella bursa-pastoris.
abstract: A novel cor gene was cloned from Capsella bursa-pastoris (designated Cbcor15b) by RACE-PCR. The full-length cDNA of Cbcor15b was 652bp and contained a 417bp open reading frame (ORF) encoding a 139-amino acid hydrophilic protein. Multiple alignments showed that Cbcor15b had high similarity with other cold-regulated genes from Arabidopsis thaliana (cor15b, cor15a), Brassica napus (bn115, bn19 and bn26) and genes encoding late embryogenesis abundant (LEA) proteins. The predicted CbCOR15B protein was found to have a potential chloroplast signal sequence cleavage site, two cAMP- and cGMP-dependent protein kinase (PKA and PKG) phosphorylation sites. Cold acclimation assay showed that Cbcor15b was relevant to cold acclimation. Our study implies that Cbcor15b might have similar functions possessed by other cor genes in increasing plants' freezing tolerance.
authors.affiliation: State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SITU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Sixiu
authors.initials: S
authors.lastname: Liu
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wang
authors.forename: Zhengqiu
authors.initials: Z
authors.lastname: Fan
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Pang
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Sun
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Wang
authors.forename: Kexuan
authors.initials: K
authors.lastname: Tanga
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D029687:Capsella / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D029681:Arabidopsis Proteins; C116166:COR15 protein, Arabidopsis; D018076:DNA, Complementary; D010940:Plant Proteins