title: [Effects of the box-3 region of the LIFRalpha-chain cytoplasmic domain (gp190CT3) on the proliferation and differentiation of HL-60 cells].
abstract: OBJECTIVE
  To study the effects of Box-3 region of the leukemia inhibitory factor receptor (LIFR) alpha-chain cytoplasmic domain on the proliferation and differentiation of HL-60 cells.
  
  
  METHODS
  Expression vector of gp190CT3 was constructed and expressed in HL-60 cells. The expression level of gp190CT3 was assayed by immunocytochemistry. The growth of wild type and gp190CT3 transfected HL-60 cells were examined under microscope. The PCNA levels were assayed by Western blot, and the levels of CD15 by flow cytometry.
  
  
  RESULTS
  The gp190CT3 transfected HL-60 cells were enlarged in size and their proliferation was slower than that of wild type. The expression level of PCNA was down-regulated while the level of CD15 up-regulated in transfected HL-60 cells as compared with that of the wild type cells.
  
  
  CONCLUSIONS
  The Box-3 region of the leukemia inhibitory factor receptor alpha-chain cytoplasmic domain (gp190CT3) participates the LIFR signal transduction in inhibiting the growth and inducing the differentiation of HL-60 cells.
authors.affiliation: Histology and Embryology Department, Second Military Medical University, Shanghai 200433, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Shan-rong
authors.initials: SR
authors.lastname: Liu
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Tang
authors.forename: Feng-mei
authors.initials: FM
authors.lastname: Wang
authors.forename: Hou-qi
authors.initials: HQ
authors.lastname: Liu
mesh_terms: D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation; D005822:Genetic Vectors / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D053606:Receptors, OSM-LIF / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D016256:Lewis X Antigen; D018809:Proliferating Cell Nuclear Antigen; D053606:Receptors, OSM-LIF