title: [Study on expression of adherent proteins related to regulation of hematopoiesis in long-term cultured human bone marrow stromal cells].
abstract: OBJECTIVE
  To study the molecular basis of bone marrow stromal cell supporting hematopoiesis.
  
  
  METHODS
  Immunoelectron microscopic localization of adherent protein related to regulation of hematopoiesis was performed by immunocolloidal gold labelling technique in the adherent layer of long-term cultured human bone marrow stromal cells (LTCHBMSC).
  
  
  RESULTS
  Adherent proteins were found to be expressed on LTCHBMSC. Double labelling revealed co-expression of these proteins and some specific markers for endothelial cells (vWF) and fibroblasts (Fb-sp). It was suggested that endothelial cells and fibroblasts in LTCHBMSC could synthesize and secrete adherent proteins, which bound to the surface of stromal cells and extracellular matrix.
  
  
  CONCLUSIONS
  Adherent proteins and all kinds of hematopoietic growth factors secreted by stromal cells constitute a complex network supporting hematopoiesis.
authors.affiliation: Institute of Hematology, CAMS and PUMC, Tianjin 300020.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Qi
authors.forename: E
authors.initials: E
authors.lastname: de Wynter
authors.forename: N G
authors.initials: NG
authors.lastname: Teata
authors.forename: T M
authors.initials: TM
authors.lastname: Dexter
authors.forename: T D
authors.initials: TD
authors.lastname: Allen
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006046:Gold / Q000378:metabolism; D006410:Hematopoiesis; D006801:Humans; D008854:Microscopy, Electron; D017154:Stromal Cells / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D015815:Cell Adhesion Molecules; D006046:Gold