Spatial and temporal control of expression of therapeutic genes using heat shock protein promoters.
Heat-shock protein promoters, particularly hsp70, have been used for gene therapy strategies because of their efficiency and the possibility of induction by external heat. This review describes some of the characteristics of hsp70 promoters that make them attractive for use in gene therapy. The human hsp70B promoter is especially promising because of its dose response effect with regard to temperature. Spatial and temporal control of transgene expression using hsp70 promoters necessitates non-invasive methods of local heat deposition and accurate local control of temperature. Special emphasis is given to Focused Ultrasound heating guided by Magnetic Resonance temperature mapping.
Rome
Claire
C
Laboratory for Molecular and Functional Imaging, ERT CNRS/Université Victor Segalen, Case 117, 146 rue Léo Saignat, F-33076 Bordeaux, France.
Couillaud
Franck
F
Moonen
Chrit T W
CT
D045744:Cell Line, Tumor; D003201:Computers; D004305:Dose-Response Relationship, Drug; D005334:Fever; D015316:Genetic Therapy / Q000379:methods*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D008279:Magnetic Resonance Imaging; D011401:Promoter Regions, Genetic; D013696:Temperature; D013997:Time Factors; D019076:Transgenes
D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins