[Molecular cloning and sequence analyzing of cytoplasmic ribosomal protein gene OsRPS7 from rice (Oryza sativa)].
Using the cDNA of rye cytoplasmic ribosomal protein ScRPS7 as a query probe, a highly homologous rice genomic contig was obtained from Huada rice genome database. The full-length cDNA sequence of rice cytoplasmic ribosomal protein S7 was assembled by informatics based on the contig. Furthermore, with the two primers designed according to this assembled cDNA, the full-length cDNA of rice ribosomal protein was cloned by RT-PCR and named as OsRPS7. The cDNA was 919bp in length and contained a complete Open Reading Frame (ORF) of 576bp, encoding a protein of 192 amino acid residues. The deduced amino acids of OsRPS7 showed 88%,72% and 72% identity with those from Secale cereale, Arabidopsis thaliana and Brassica oleracea, respectively. The genome structure of OsRPS7 was analyzed, and its function was predicted in this paper.
Gu
Zhi-Min
ZM
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. zhmgu.student@sina.com
Wang
Jian-Fei
JF
Huang
Ji
J
Zhang
Hong-Sheng
HS
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D001937:Brassica / Q000235:genetics; D003001:Cloning, Molecular; D020451:Contig Mapping; D003593:Cytoplasm / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis; D040342:Genetic Structures / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D012269:Ribosomal Proteins / Q000235:genetics*; D012434:Secale / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D018744:DNA, Plant; D012269:Ribosomal Proteins; C037442:ribosomal protein S7