title: The crystal structure of human adenylate kinase 6: An adenylate kinase localized to the cell nucleus.
abstract: Adenylate kinases (AKs) play important roles in nucleotide metabolism in all organisms and in cellular energetics by means of phosphotransfer networks in eukaryotes. The crystal structure of a human AK named AK6 was determined by in-house sulfur single-wavelength anomalous dispersion phasing methods and refined to 2.0-A resolution with a free R factor of 21.8%. Sequence analyses revealed that human AK6 belongs to a distinct subfamily of AKs present in all eukaryotic organisms sequenced so far. Enzymatic assays show that human AK6 has properties similar with other AKs, particularly with AK5. Fluorescence microscopy showed that human AK6 is localized predominantly to the nucleus of HeLa cells. The identification of a nuclear-localized AK sheds light on nucleotide metabolism in the nucleus and the energetic communication between mitochondria and nucleus by means of phosphotransfer networks.
authors.affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering and Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
authors.forename: Liya
authors.initials: L
authors.lastname: Wang
authors.forename: Matthew
authors.initials: M
authors.lastname: Bennett
authors.forename: Yuhe
authors.initials: Y
authors.lastname: Liang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zheng
authors.forename: Fei
authors.initials: F
authors.lastname: Lu
authors.forename: Lanfen
authors.initials: L
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Nan
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.forename: Staffan
authors.initials: S
authors.lastname: Eriksson
authors.forename: Chuanmao
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Su
mesh_terms: D021581:Active Transport, Cell Nucleus; D000263:Adenylate Kinase / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D009711:Nucleotides; D000263:Adenylate Kinase; C118782:adenylate kinase 5