[An efficient macro-method of genomic DNA isolation from Actinidia chinensis leaves].
It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharose, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.
Chen
Kun-Song
KS
Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Huajiachi Campus, Hangzhou 310 029, China. akun@zju.edu.cn
Li
Fang
F
Xu
Chang-Jie
CJ
Zhang
Shang-Long
SL
Fu
Cheng-Xin
CX
D029042:Actinidia / Q000737:chemistry / Q000235:genetics*; D015139:Blotting, Southern; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D018744:DNA, Plant / Q000302:isolation & purification*; D018745:Genome, Plant; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics
D002593:Cetrimonium Compounds; D018744:DNA, Plant; D000077286:Cetrimonium