[Cloning and expression of ethylene receptor gene Ad-ETR1 from kiwifruit].
According to the conserved amino acid sequence from ethylene receptors in other plants, a pair of degenerate primers was designed and a 657-bp cDNA fragment encoding an ethylene receptor fruit was obtained by RT-PCR from ripening kiwifruit (Actinidia deliciosa cv. Bruno) (Fig. 1). The cDNA fragment encoding 219 amino acids was named Ad-ETR1, and its sequences shared high similarity at both nucleotide and polypeptide level with the sequences from the plants of Arabidopsis, tomato, persimmon, avocado, citrus and peach (Fig. 2, Table 1). Northern blot analysis indicated that the levels of Ad-ETR1 mRNA increased during kiwifruit ripening and reached peak at 144 h after treatment, then dropped immediately. The expression of Ad-ETR1 could be induced by ethylene treatment, while acetylsalicylic acid (ASA) treatment inhibited its expression (Fig. 4). In consistency with the changes of Ad-ETR1 mRNA, ethylene or ASA treatment had marked effects on the kiwifruit ethylene production and fruit softening (Fig. 3). The significance of these results was discussed.
Wei
Shao-Chong
SC
Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Chen
Kun-Song
KS
Zhang
Yu
Y
Zhang
Wang-Shu
WS
Ding
Jian-Guo
JG
D029042:Actinidia / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005638:Fruit / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant