title: Identification of an unusual VanA element in glycopeptide-resistant Enterococcus faecium in Brazil following international transfer of a bone marrow transplant patient.
abstract: A vancomycin-resistant Enterococcus (VRE) was isolated from a blood culture of a patient in a Brazilian hospital who had a treatment history of a bone marrow transplant in the USA. The organism was identified as Enterococcus faecium, which exhibited an MIC (minimum inhibitory concentration) >or= 256 microg/mL for vancomycin. This was confirmed by E-test and the vanA gene was detected by PCR. Overlapping PCR revealed a left IR deletion and an additional 1.5 kb fragment between vanSH genes. DdeI digestion of vanRSHAX genes showed the determinant to be a T type variant, and the element was cloned and sequenced. These results revealed an IS1251 downstream of nucleotide 5820 of the VanA element. Insertions like this have not been reported previously in Brazil, but have been detected in the USA. The genotype and association with a patient previously treated in the USA suggest that this VRE was introduced from abroad, probably through inter-hospital strain spread.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
authors.forename: I L B C
authors.initials: IL
authors.lastname: Camargo
authors.forename: P F
authors.initials: PF
authors.lastname: Del Peloso
authors.forename: C F
authors.initials: CF
authors.lastname: Da Costa Leite
authors.forename: G H
authors.initials: GH
authors.lastname: Goldman
authors.forename: A L C
authors.initials: AL
authors.lastname: Darini
mesh_terms: D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics*; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D001938:Brazil; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D004251:DNA Transposable Elements; D016984:Enterococcus faecium / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014187:Transportation of Patients; D014481:United States; D020713:Vancomycin Resistance
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases