[Establishment and evaluation on the method for platelet intracellular trehalose determination].
This study was aimed to establish an applicable, convenient and rapid method for platelet intracellular trehalose determination, so that the technique of trehalose loading could be optimized and used in research on freeze-drying platelets. Protein from loaded-trehalose platelets was deposited by using trichloroacetic acid, trechalose concentration in platelets was determinated by sulfuric-anthrone reaction and was analyzed by high performance liquid chromatography (HPLC). The results showed that when platelets were loaded with 1.7% of trehalose in loading solution, intracellular concentration determinated by sulfuric-anthrone reaction was 0.22% and derterminated by HPLC was 0.2%. The recovery rate of trehalose determinated by sulfuric-anthrone reaction was 100.7% and stability and repeatability of results were good. In conclusion, the method is convenient, rapid, accurate and highly sensitive for determination of platelet intracellar trehalose.
Zhou
Jun
J
Department of Blood Transfusion, Gerneral Hospital of PLA, Center of Clinical Transfusion of PLA, Beijing 100853, China.
Liu
Jing-Han
JH
Ouyang
Xi-Lin
XL
Zhuang
Yuan
Y
Li
Xi-Jin
XJ
D001792:Blood Platelets / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D015203:Reproducibility of Results; D013464:Sulfuric Acids / Q000737:chemistry; D014199:Trehalose / Q000032:analysis*
D013464:Sulfuric Acids; D014199:Trehalose; C033158:sulfuric acid