[Expression of iron responsive element binding protein mRNA and analysis of aconitase activity in iron deficiency anemia rats].
OBJECTIVE
To explore the regulatory mechanism of iron responsive element binding protein (IRE-BP) in iron metabolism.


METHODS
The expression level of IRE-BP mRNA and the aconitase activity were studied in iron deficiency anemia (IDA) rat model.


RESULTS
No distinct change of IRE-BP mRNA expression was observed in iron deficiency rats. The aconitase activity of liver cytoplasmic protein was much lower in iron deficiency rats than that in controls and treatment of liver cytoplasmic protein with FeSO4 resulted in a strong stimulation of the enzymatic activity.


CONCLUSIONS
Both posttranslational and posttranscriptional regulation of IRE-BP may take part in iron metabolism balance.
Li
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X
Department of Hematology, Second Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041.
Li
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F
Liao
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Q
D000154:Aconitate Hydratase / Q000378:metabolism*; D018798:Anemia, Iron-Deficiency / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D005260:Female; D005786:Gene Expression Regulation; D007501:Iron / Q000378:metabolism; D035925:Iron-Regulatory Proteins / Q000235:genetics*; D008099:Liver / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D035925:Iron-Regulatory Proteins; D012333:RNA, Messenger; D007501:Iron; D000154:Aconitate Hydratase