[Diagnosis of hepatitis E virus infection in sporadic acute hepatitis].
The solid-phase enzyme immunoassay (EIA) was used for detecting anti-HEV immunoglobulin G (IgG) and M (IgM) by using a recombinant chimeric antigen (ORF3-2) from the open reading frame (ORF) 2 and 3 of hepatitis E virus gene. These sera were collected from the patients with sporadic acute viral hepatitis. The positive rate for anti-HEV IgG was 15.22% (7) among 46 patients with acute hepatitis, 5 of the 7 IgG positive sera were also positive (71.4%) for anti-HEV IgM. 91.8% (93) and 76.4% (78) were positive separately for anti-HEV IgG and IgM among 102 patients of suspected HEV infection (these patients were negative for anti-HAV, anti-HBV and anti-HCV), about 2/3 patients of HEV infection detected were simultaneously HEV-IgG and IgM positive. In control group of 98 normal persons, 4 (4.08%) were positive for anti-HEV IgG, but none were IgM positive. Paired sera of the patients in the acute phase and convalescent phase were detected, the titers of anti-HEV IgG with 4-16 fold decrease were seen in 6 cases and with 2-fold rise in 1 case.
Jiang
Y
Y
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Bi
S
S
Li
J
J
Lu
J
J
Liao
S
S
Zhao
H
H
Wang
P
P
Zhan
M
M
Liu
C
C
D000208:Acute Disease; D000914:Antibodies, Viral / Q000276:immunology; D016751:Hepatitis E / Q000175:diagnosis* / Q000276:immunology* / Q000821:virology; D016752:Hepatitis E virus / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M