[Coxsackie virus B types were discriminated by RT-PCR].
OBJECTIVE
To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.


METHODS
A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.


RESULTS
This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.


CONCLUSIONS
The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.
Li
Zhen-yong
ZY
Laboratory of Chemobiology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China. zhenyongl@sabc.com.cn
Li
Zhi-tao
ZT
Feng
Yan-ming
YM
Shao
Da-xiao
DX
Zhao
Da-peng
DP
Cui
Tian-xing
TX
Yang
Guo-cui
GC
Qu
Ling-bo
LB
Zhao
Yu-fen
YF
D017931:DNA Primers; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D007075:Immunoglobulin M