Cetyl-pyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage.
Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subjected to long-term storage received from remote areas of the world.
Pardini
Manuela
M
Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Varaine
Francis
F
Iona
Elisabetta
E
Arzumanian
Erchanik
E
Checchi
Francesco
F
Oggioni
Marco Rinaldo
MR
Orefici
Graziella
G
Fattorini
Lanfranco
L
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002594:Cetylpyridinium / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D013183:Sputum / Q000187:drug effects* / Q000382:microbiology*; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D000891:Anti-Infective Agents, Local; D003470:Culture Media; D002594:Cetylpyridinium