Granulocyte colony-stimulating factor decreases tumor necrosis factor production in whole blood: role of interleukin-10 and prostaglandin E(2).
Previous reports have indicated that the administration of granulocyte colony-stimulating factor (G-CSF) decreases ex vivo tumor necrosis factor (TNF) production in humans. In this study, we report that daily pretreatment of mice with G-CSF for three days decreases ex vivo lipopolysaccharide (LPS)-induced TNF production in whole blood. Conversely, production of interleukin-10 (IL-10) and prostaglandin E(2) (PGE(2)) is increased. The inhibitory effect of G-CSF pretreatment on TNF production is partially reversed by addition of an anti-IL-10 antibody, and completely reversed by combined addition of anti-IL-10 antibody and the cyclooxygenase (COX) inhibitor, ketoprofen. These results suggest that G-CSF decreases TNF production in this experimental model by increasing production of IL-10 and PGE(2), which are both known inhibitors of TNF production.
Agnello
Davide
D
"Mario Negri" Institute for Pharmacological Research, Milan, Italy.
Mascagni
Patrizia
P
Bertini
Riccardo
R
Villa
Pia
P
Senaldi
Giorgio
G
Ghezzi
Pietro
P
D000818:Animals; D001773:Blood Cells / Q000502:physiology*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D066298:In Vitro Techniques; D007279:Injections, Subcutaneous; D016753:Interleukin-10 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D048069:Tumor Necrosis Factors / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D048069:Tumor Necrosis Factors; D016753:Interleukin-10; D016179:Granulocyte Colony-Stimulating Factor; D015232:Dinoprostone