[Study on experimental induction of fluconazole resistance in Candida albicans].
To investigate the phenotype and genotype variation between the Fluconazole resistant C. albicans isolates and the corresponding susceptible ones, our research established a resistance-induction mode in vitro. Comparisons were done on drug resistance maintainability, metabolic profile and the doubling time in the logarithmic growth phase. Genotypes were determined by ERIC-PCR. The Fluconazole resistant isolates appeared in strain 435, A06, B07 and C01 from total 22 clinical Fluconazole susceptible isolates after being incubated for 45-80 days in YEPD broth with increasing Fluconazole concentration. The parent isolates had a same metabolic profile and a similar growth doubling time to their filial generation. The same ERIC-PCR profiles were also found between the susceptible parents and their resistant filial isolates. The resistant isolates maintained drug resistance for 24 days after growing on drug-free medium. It was supposed that candida albicans had a latent capacity to evolve resistance to azoles under a certain antifungal drug selective pressure, and the acquired resistance could maintain in drug-free media for a certain period. The resistant isolate with no adaptive cost may be prone to vogue among people. ERIC-PCR could be used in epidemiological study as a stable marker.
Zhu
Yu Ning
YN
Woman's Hospital, School of Medicine, Zhejiang University, Hangzhou.
Lu
Shi Ming
SM
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction
D000935:Antifungal Agents; D004271:DNA, Fungal; D015725:Fluconazole