title: Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite.
abstract: To investigate the role of clathrin-mediated trafficking during the Leishmania lifecycle, open reading frames encoding clathrin heavy chain and the beta-adaptins, major components of the adaptor complexes, have been analysed both in silico and experimentally. The Leishmania genome encodes three beta-adaptins, which arose at a time predating speciation of these divergent trypanosomatids. Unlike Trypanosoma brucei, both clathrin heavy chain and beta-adaptin1 are constitutively expressed throughout the Leishmania life cycle. Clathrin relocalises in amastigotes relative to promastigotes, consistent with developmental alterations to the morphology of the endo-membrane system.
authors.affiliation: Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College, London SW7 2AY, UK. p.w.denny@durham.ac.uk
authors.forename: Paul W
authors.initials: PW
authors.lastname: Denny
authors.forename: Gareth W
authors.initials: GW
authors.lastname: Morgan
authors.forename: Mark C
authors.initials: MC
authors.lastname: Field
authors.forename: Deborah F
authors.initials: DF
authors.lastname: Smith
mesh_terms: D033966:Adaptor Protein Complex beta Subunits / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D015153:Blotting, Western; D033922:Clathrin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D018503:Genome, Protozoan; D006056:Golgi Apparatus / Q000737:chemistry; D018320:Leishmania major / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016366:Open Reading Frames; D010802:Phylogeny
chemical_list: D033966:Adaptor Protein Complex beta Subunits; D000913:Antibodies, Protozoan; D033922:Clathrin Heavy Chains