title: Novel insertion sequence-like elements in phytoplasma strains of the aster yellows group are putative new members of the IS3 family.
abstract: Novel insertion sequence (IS)-like elements were isolated and characterized from phytoplasma strains in the aster yellows (AY) group (16SrI). The IS-like elements were cloned from phytoplasma strains AY1 and NJAY or PCR-amplified from 15 additional strains representing nine subgroups in the AY group using primers based on sequences of the putative transposases (Tpases). All IS-like elements contained sequences encoding similar Tpases of 321 amino acids (320 for strain CPh). Substantial amino acid sequence variability suggested multiple species of Tpases or IS-like elements exist in the AY phytoplasma group. These Tpases have an identical DDE motif that is most similar to the DDE consensus of Tpases in the IS3 family.
authors.affiliation: Molecular Plant Pathology Laboratory, USDA, ARS, Beltsville, MD 20705, USA. leeim@ba.ars.usda.gov
authors.forename: I-M
authors.initials: IM
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: K D
authors.initials: KD
authors.lastname: Bottner
mesh_terms: D000595:Amino Acid Sequence; D031173:Aster Plant / Q000382:microbiology*; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D045582:Phytoplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology; D019895:Transposases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C473498:IS3 transposase; D019895:Transposases