Quantal size is independent of the release probability at hippocampal excitatory synapses.
Short-term synaptic plasticity changes the reliability of transmission during repetitive activation and allows different neuronal ensembles to encode distinct features of action potential trains. Identifying the mechanisms and the locus of expression of such plasticity is essential for understanding neuronal information processing. To determine the quantal parameters and the locus of alterations during short-term plasticity of cortical glutamatergic synapses, EPSCs were evoked in hippocampal oriens-alveus interneurons by CA1 pyramidal cells. The robust short-term facilitation of this connection allowed us to examine the transmission under functionally relevant but widely different release probability (P(r)) conditions. Paired whole-cell recordings permitted the functional and post hoc morphological characterization of the synapses. To determine the quantal size (q), the P(r), and the number of functional release sites (N(F)), two independent quantal analysis methods were used. Light and electron microscopy were performed to identify the number of synaptic junctions (N(EM)) between the recorded cells. The mean number of functional release sites (N(F(f)) = 2.9 +/- 0.4; n = 8) as inferred from a simple binomial model with no quantal variance agreed well with the mean of N(EM) (2.8 +/- 0.8; n = 6), but N(F(f)) never matched N(EM) when they were compared in individual pairs; however, including quantal variance in the model improved the functional prediction of the structural data. Furthermore, an increased P(r) (4.8 +/- 0.8-fold) fully accounted for the marked short-term facilitation of EPSCs (5.0 +/- 0.7-fold), and q was independent of P(r). Our results are consistent with the "one-release site, one-vesicle" hypothesis.
Biró
Agota A
AA
Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, 1083 Budapest, Hungary.
Holderith
Noémi B
NB
Nusser
Zoltan
Z
D000818:Animals; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D018698:Glutamic Acid / Q000494:pharmacology; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D007395:Interneurons / Q000502:physiology; D008297:Male; D008959:Models, Neurological; D009473:Neuronal Plasticity / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011336:Probability; D017966:Pyramidal Cells / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013572:Synaptic Vesicles / Q000502:physiology; D013997:Time Factors
D018377:Neurotransmitter Agents; D018698:Glutamic Acid