[The implication of circulating CD34+ Thy-1(+) cells measurement during autologous peripheral blood stem cell mobilization].
OBJECTIVE
To estimate the amount of peripheral blood stem cells(PBSC) right during mobilization and determine the optimal time for PBSC collection.


METHODS
A serial measurement of circulating CD34+ Thy-1(+) cells were carried out during PBSC mobilization by chemotherapy and granulocyte colony stimulating factor(G-CSF) using dual-color direct immunofluorescence flow cytometry. Colony-culture in vitro was performed at the same time to evaluate the clonogenic capacity of the peripheral blood progenitor cells (PBPCs).


RESULTS
Circulating CD34+ Thy-1(+) cells, CD34+ cells and colony-forming cells increased by 48.6-, 50- and 53.1- fold, respectively, after mobilization. The peak levels were reached at 12 approximately 14 days after chemotherapy. The proportion of CD34+ cells and the candidate stem cells, CD34+ Thy-1(+) subset, in mononuclear cells (MNCs) exceeded baseline by 10.5- and 13.8- fold, respectively. The percentage of CD34+ cells coexpressing Thy-1 was highest in the earlist phase of mobilization.


CONCLUSIONS
PBSCs, especially primitive progenitor cells, increased significantly during mobilization with chemotherapy and G-CSF. Flow cytometric assay of circulating CD34 Thy-1(+) cells promptly provides a more reliable indicator for clinical collection of PBSC.
Yang
B
B
Department of Hematology, The Third Clinical School of Medicine, Beijing Medical University, Beijing 100083.
Wang
L
L
Chen
P
P
D000328:Adult; D018952:Antigens, CD34 / Q000097:blood* / Q000378:metabolism*; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000097:blood* / Q000378:metabolism*; D055815:Young Adult
D018952:Antigens, CD34; D018800:Thy-1 Antigens