[Genetic polymorphisms of five STR loci on chromosome 21 in Chinese Han population].
To elucidate the genetic polymorphisms of five STR loci on chromosome 21 in Chinese Han population and construct a preliminary database, EDTA-blood specimens were collected from unrelated individuals in Beijing. The DNAs were extracted with Chelex method and were amplified by PCR. The PCR products were analyzed by the PAG electrophoresis or by the approach of the automated fluorescent detection. The five STR loci consist of simple repeat motif and its distributions of genotypes are agreement with Hardy-Weinberg equation. Its polymorphism information content is all over 0.50. The obtained data can not only be used as evidences for genetic diagnosis of Down Syndrome, but also for calculating the probabilities in the paternity test and individual identification.
Chen
Zhen-Bin
ZB
The Laboratory of Electron Microscopy of Fujian Medical University, Fuzhou 350 004, China. czb86233@163.com
Zhu
Jin-Ling
JL
Yan
Mei
M
Liang
Yan
Y
Zhou
Yan
Y
Tan
Shu-Zhen
SZ
Xiao
Bai
B
Liu
Jing-Zhong
JZ
D000483:Alleles; D044466:Asian People; D002681:China / Q000208:ethnology; D002891:Chromosomes, Human, Pair 21; D018076:DNA, Complementary / Q000235:genetics; D004314:Down Syndrome / Q000235:genetics; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D010334:Paternity; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D018076:DNA, Complementary; D005819:Genetic Markers