Delivery of siRNA and siRNA expression constructs to adult mammals by hydrodynamic intravascular injection.
Extensive use of RNA interference in mammals has been hindered by the inability to effectively deliver small interfering RNAs (siRNAs) or DNA-based constructs designed to express siRNAs. In this chapter, we describe the high-pressure or hydrodynamic intravascular injection technique used to deliver these nucleic acids to mice and nonhuman primates. Emphasis is placed on the use of this technique for delivery to the liver.
Lewis
David L
DL
Mirus Corporation, Madison, WI 53719, USA.
Wolff
Jon A
JA
D000818:Animals; D007275:Injections, Intravenous; D008099:Liver / Q000378:metabolism; D008322:Mammals; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000378:metabolism
D034741:RNA, Small Interfering