[Comparison of denatured and non-denatured PAGE-silver-staining detection of PCR product of microsatellites].
The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels.
Qu
Lu-Jiang
LJ
College of Animal Science and Technology, China Agricultural University, Beijing 100 094, China.
Li
Xian-Yao
XY
Du
Zhi-Qiang
ZQ
Zhang
Long-Chao
LC
Yang
Ning
N
D000818:Animals; D002645:Chickens / Q000145:classification / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016622:Silver Staining
D004247:DNA