title: Oxidation of glycerol-3-phosphate in porcine and bovineadrenal cortex mitochondria.
abstract: The capabilities of porcine adrenal cortex mitochondria to oxidize glycerol-3-phosphate (GP) were studied. In comparison with bovine adrenal cortex mitochondria, porcine mitochondria oxidized GP about three times more actively (18.9 vs 6.1 nmol O(2)/min per mg protein in the presence of ADP) and the activity of mitochondrial glycerol-3-phosphate dehydrogenase was about four times higher (33.4 vs 8.2 nmol/min per mg protein). In porcine adrenal cortex mitochondria we found similar values for succinate and GP oxidation both in the absence and presence of ADP or deoxycorticosterone (DOC). Rotenone sensitivity of DOC stimulation of GP oxidation indicated that porcine adrenal cortex mitochondria are able to oxidize GP and thus to generate NADPH from GP, presumably via reverse electron transport followed by energy-dependent NADH-NADP transhydrogenation.
authors.affiliation: Department of Bioenergetics, Jedrzej Sniadecki University School of Physical Education and Sport, Gdańsk, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Popinigis
authors.forename: Jedrzej
authors.initials: J
authors.lastname: Antosiewicz
authors.forename: Jan Jacek
authors.initials: JJ
authors.lastname: Kaczor
authors.forename: Hana
authors.initials: H
authors.lastname: Rauchová
authors.forename: Giorgio
authors.initials: G
authors.lastname: Lenaz
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D010084:Oxidation-Reduction; D019802:Succinic Acid / Q000378:metabolism; D013552:Swine / Q000378:metabolism
chemical_list: D005994:Glycerophosphates; C029620:alpha-glycerophosphoric acid; D019802:Succinic Acid; D005993:Glycerolphosphate Dehydrogenase