title: Evaluation of fatty acid metabolism in hearts after ischemia-reperfusion injury using a dual-isotope autoradiographic approach and tissue assay for metabolites of tracer.
abstract: We investigated whether changes in myocardial uptake of fatty acid tracer after reperfusion following transient myocardial ischemia were closely related to alterations in intracellular fatty acid oxidation.
  
  
  METHODS
  Using a fatty acid tracer of (131)I- and (125)I-labeled 15-(p-iodophenyl)-9-methylpentadecanoic acid (9MPA), the myocardial uptake and metabolites were determined by dual-tracer autoradiography and thin-layer chromatography in rats 3 or 14 d after reperfusion following 5 or 15 min of ischemia induced by coronary artery ligation.
  
  
  RESULTS
  9MPA metabolites processed via beta-oxidation were lower in the ischemic region (IR) than in non-IR 3 d after 5 min of ischemia, despite no reduction of tracer uptake in IR. Oxidation of 9MPA was recovered 14 d after 15 min of ischemia in association with normalization of tracer uptake in IR, whereas both uptake and oxidation of 9MPA were markedly impaired 3 d after 15 min of ischemia, accompanied by slow clearance of myocardial tracer.
  
  
  CONCLUSIONS
  Normal uptake of fatty acid tracer early after reperfusion does not always imply preserved intracellular fatty acid oxidation. However, reduction of tracer uptake might reflect impaired fatty acid oxidation.
authors.affiliation: Second Department of Internal Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Igarashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nozawa
authors.forename: Nozomu
authors.initials: N
authors.lastname: Fujii
authors.forename: Bun-ichi
authors.initials: B
authors.lastname: Kato
authors.forename: Makoto
authors.initials: M
authors.lastname: Nonomura
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuki
authors.forename: Teruo
authors.initials: T
authors.lastname: Nakadate
authors.forename: Akihiko
authors.initials: A
authors.lastname: Igawa
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Asanoi
authors.forename: Minoru
authors.initials: M
authors.lastname: Inoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inoue
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D005227:Fatty Acids / Q000378:metabolism / Q000493:pharmacokinetics*; D006321:Heart / Q000000981:diagnostic imaging*; D007462:Iodobenzenes / Q000493:pharmacokinetics*; D008657:Metabolic Clearance Rate; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000000981:diagnostic imaging* / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D007462:Iodobenzenes; D019275:Radiopharmaceuticals; C067799:4-iodophenyl-9-methylpentadecanoic acid