Assessing immunoglobulin heavy chain rearrangements in pediatric CD20-positive and CD20-negative classic Hodgkin's disease.
Approximately 15% of all cases of childhood classical Hodgkin's disease (HD) express CD20, a B-cell marker associated with immunoglobulin heavy chain rearrangements. Immunoglobulin heavy chain rearrangements in Reed-Sternberg cells could be used to assess minimal residual disease (MRD), as was shown with immunoglobulin heavy chain patient-specific primers (PSPs) in non-Hodgkin's lymphoma. The aim of this study was to analyze pediatric HD for future design of immunoglobulin heavy chain PSP for MRD detection. DNA was extracted from paraffin-embedded tissue from unstained slides of 8 pediatric CD20+ nodular sclerosis HD cases and 10 CD20-nodular sclerosis HD cases. Immunoglobulin heavy chain polymerase chain reaction and sequencing were performed on 16 of 18 cases, which had adequate DNA for further analysis. Sequence analysis from 3 cases (19% of HD cases) demonstrated unique V(D)J regions, which could potentially be used to design PSP. Unique PSPs could be used to assess MRD in advanced-stage HD specimens. Future studies should focus on improved detection and analysis of more cases to identify appropriate specimens in assessing clinical implications of MRD detection.
Shiramizu
Bruce
B
University of Hawaii, Honolulu, Hawaii, USA. bshirami@hawaii.edu
Perkins
Sherrie L
SL
Bergeron
Sharon
S
Lones
Mark
M
Kelly
Kara
K
Weiner
Michael
M
Cairo
Mitchell S
MS
D000293:Adolescent; D015703:Antigens, CD / Q000032:analysis / Q000097:blood; D018951:Antigens, CD20 / Q000032:analysis / Q000097:blood*; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D006689:Hodgkin Disease / Q000097:blood / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008297:Male; D009367:Neoplasm Staging
D015703:Antigens, CD; D018951:Antigens, CD20; D017931:DNA Primers; D004273:DNA, Neoplasm