The growth of attenuated influenza vaccine donor strains in continuous cell lines.
The growth of the Russian live attenuated influenza vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57 and B/USSR/60/69 was studied in cells of the VERO and Madin-Darby canine kidney (MDCK) lines as six-well cultures and cell factories infected at different multiplicities of infection. Yields for A/Leningrad/134/17/57 and A/Leningrad/134/47/57 were comparable in either cell line over a range of multiplicities but were about 10-fold lower than in the allantoic fluids of infected chicken embryos. For both A/Leningrad/134/47/57 and B/USSR/60/69, yields from the MDCK line were about 10-fold higher than for the VERO line. For B/USSR/60/69, yields in eggs were approximately 100-fold higher than those obtained in the MDCK line. A feature of the growth of B/USSR/60/69 was its reduced capacity to produce infectious progeny in either cell line at multiplicities of infection of 2.0 or 1.0 pfu/cell. Inhibition was due probably due to the presence of defective-interfering particles and was not detected with A/Leningrad/134/17/57 or A/Leningrad/134/47/57 in cultures of either line infected at the same multiplicities. Yields for both A/Leningrad/134/47/57 and B/USSR/60/69 in cells of the MDCK line were comparable when grown in six-well cultures or cell factories.
Audsley
J M
JM
Department of Biotechnology and Environmental Biology, RMIT University, P.O. Box 71, Bundoora, Vic. 3083, Australia.
Tannock
G A
GA
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D009980:Influenza A virus / Q000254:growth & development* / Q000502:physiology; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D014709:Vero Cells; D010948:Viral Plaque Assay; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D007252:Influenza Vaccines; D014613:Vaccines, Attenuated