title: Utilization of amylose-lipid complexes as molecular nanocapsules for conjugated linoleic Acid.
abstract: Amylose-conjugated linoleic acid (CLA) complexes were produced by water/dimethyl sulfoxide (DMSO) and KOH/HCl complexation methods. The formation of amylose V form was confirmed by X-ray diffraction (XRD), and complexes formed at 30, 60, and 90 degrees C exhibit melting temperatures exceeding 88 degrees C. Atomic force microscopy (AFM) images showed distinct difference in complex organization, with complexes formed in water/DMSO showing spherical shape with typical diameter of 150 nm. Complexes formed by KOH/HCl showed elongated structure with typical width of 43-160 nm. Water/DMSO complexes exhibit superior protection to CLA against oxidation. All complexes showed high retention of CLA in simulated stomach conditions, and the digestion of complexes by amylases results in high hydrolysis and CLA release by pancreatin and alpha-amylase. Only moderate release was detected following hydrolysis by amyloglucosidase and beta-amylase. It is therefore suggested that amylose-CLA complexes can serve as molecular nanocapsules for protection and delivery of CLA.
authors.affiliation: Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
authors.forename: Inbal
authors.initials: I
authors.lastname: Lalush
authors.forename: Hagit
authors.initials: H
authors.lastname: Bar
authors.forename: Imad
authors.initials: I
authors.lastname: Zakaria
authors.forename: Sigal
authors.initials: S
authors.lastname: Eichler
authors.forename: Eyal
authors.initials: E
authors.lastname: Shimoni
mesh_terms: D000681:Amylases / Q000737:chemistry; D000688:Amylose / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D019787:Linoleic Acid / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction; D010179:Pancreas / Q000201:enzymology; D013696:Temperature; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D008055:Lipids; D000688:Amylose; D019787:Linoleic Acid; D000681:Amylases; D006851:Hydrochloric Acid