The aminoesters as inhibitors of plasma membrane H+-ATPase in the yeast Saccharomyces cerevisiae.
A set of oxalates of alpha-dimethylamino fatty acids n-alkyl esters (MEM-ns and n-MEM-8s) and n-dodecyl-N,N-dimethylalaninate (DMAL-12s) were synthesized. Their activities on the growth, transport, and ATPases from the yeast Saccharomyces cerevisiae were compared. The compounds differ in the number of carbon atoms in their aliphatic chain and in the position of that chain in their molecular structure. The tested aminoesters with twelve carbon atoms (MEM-10s and DMAL-12s) appeared to have the highest level of activity. These drugs inhibited plasma membrane H+-ATPase, but no inhibition of mitochondrial ATPase was observed. Under nitrogen-derepressed conditions, the aminoesters inhibited amino acid uptake by yeast cells.
Obłak
Ewa
E
Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 65/73, 51-148 Wrocław, Poland.
Lachowicz
Tadeusz M
TM
Luczyński
Jacek
J
Witek
Stanisław
S
D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D003651:Decanoates / Q000737:chemistry / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D010070:Oxalates / Q000737:chemistry / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors*
D000935:Antifungal Agents; D003651:Decanoates; D004791:Enzyme Inhibitors; D010070:Oxalates; D029701:Saccharomyces cerevisiae Proteins; C085388:dodecyl 2-(N,N-dimethylamino)propionate; D006180:Proton-Translocating ATPases; D000409:Alanine