title: Cytotoxic activity of nepetin, a flavonoid from Eupatorium ballotaefolium HBK.
abstract: The present study evaluated the cytotoxic activity of nepetin and quercetin-3-O-glucoside, compounds isolated from the aerial parts of Eupatorium ballotaefolium. The antimitotic activity was determined as the ability to inhibit sea urchin eggs development and five tumor cells lines growth. Moreover, the activities of these compounds were compared to quercetin in the same models. Nepetin inhibited the proliferation of the five tumor cell lines, once quercetin-3-O-glucoside did not present any activity even at the highest tested concentration and quercetin only inhibited proliferation of the B16 cell line. On the sea urchin assay, nepetin and quercetin induced a dose-dependent inhibition on egg development, while quercetin-3-O-glucoside did not modify normalegg cleavage, even at the highest tested concentration (100 microg/ml).
authors.affiliation: Curso de Pós-Graduação em Farmacologia, Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: G C G
authors.initials: GC
authors.lastname: Militão
authors.forename: M R J R
authors.initials: MR
authors.lastname: Albuquerque
authors.forename: O D L
authors.initials: OD
authors.lastname: Pessoa
authors.forename: C
authors.initials: C
authors.lastname: Pessoa
authors.forename: M E A
authors.initials: ME
authors.lastname: Moraes
authors.forename: M O
authors.initials: MO
authors.lastname: de Moraes
authors.forename: L V
authors.initials: LV
authors.lastname: Costa-Lotufo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D036602:Eupatorium / Q000737:chemistry*; D047309:Flavones; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012617:Sea Urchins
chemical_list: D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D047309:Flavones; D005419:Flavonoids; C016527:isoquercitrin; D011794:Quercetin; C503624:eupafolin