title: The inactivation of phospholipase A2 by scalaradial: a biomimetic study by electrospray mass spectrometry.
abstract: A biomimetic approach was employed to shed light on the nature of chemical reactions occurring in the covalent inactivation of phospholipase A(2) (PLA(2)) by scalaradial (1), a marine dialdehyde terpenoid endowed with potent anti-inflammatory activity. To this end, a detailed study of the reaction profile between the nitrogenous nucleophile isopropylamine and scalaradial was performed under biologically relevant conditions.
authors.affiliation: Dipartimento di Scienze Farmaceutiche and Centro Regionale di Competenza in Diagnostica e Farmaceutica Molecolari, Università di Salerno, via Ponte Don Melillo, 84084, Fisciano (SA), Italy.
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Monti
authors.forename: Agostino
authors.initials: A
authors.lastname: Casapullo
authors.forename: Raffaele
authors.initials: R
authors.lastname: Riccio
authors.forename: Luigi
authors.initials: L
authors.lastname: Gomez-Paloma
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006718:Homosteroids / Q000737:chemistry*; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D011437:Propylamines / Q000737:chemistry*; D054830:Sesterterpenes; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013729:Terpenes / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006718:Homosteroids; D011437:Propylamines; D054830:Sesterterpenes; D013729:Terpenes; C071197:scalaradial; D010741:Phospholipases A; D054467:Phospholipases A2; C035263:2-propylamine