Insight into pollutant bioavailability and toxicity using Raman confocal microscopy.
Raman confocal microscopy was used to discriminate between cultures of Burkholderia xenovorans LB400 exposed to four different common environmental pollutants: phenanthrene, dodecane, 3-chlorobiphenyl and pentachlorophenol. Evidence is presented for the application of Raman spectroscopy as a bioassay for pollutant bioavailability and toxicity.
Singer
Andrew C
AC
Environmental Biotechnology Section, Centre for Ecology and Hydrology-Oxford, Mansfield Road, Oxford, OX1 3SR, UK. acsi@ceh.ac.uk
Huang
Wei E
WE
Helm
Jana
J
Thompson
Ian P
IP
D000473:Alkanes / Q000493:pharmacokinetics / Q000633:toxicity; D001682:Biological Availability; D001713:Biphenyl Compounds / Q000493:pharmacokinetics / Q000633:toxicity; D019117:Burkholderia / Q000187:drug effects* / Q000378:metabolism*; D004785:Environmental Pollutants / Q000493:pharmacokinetics* / Q000633:toxicity*; D018613:Microscopy, Confocal; D009930:Organic Chemicals / Q000493:pharmacokinetics* / Q000633:toxicity*; D010416:Pentachlorophenol / Q000493:pharmacokinetics / Q000633:toxicity; D010616:Phenanthrenes / Q000493:pharmacokinetics / Q000633:toxicity; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman
D000473:Alkanes; D001713:Biphenyl Compounds; D004785:Environmental Pollutants; D009930:Organic Chemicals; D010616:Phenanthrenes; C007548:n-dodecane; C031181:phenanthrene; D010416:Pentachlorophenol; C034280:3-chlorobiphenyl