Study of the reaction between the nucleic acid and Y-BPMPHD-CTMAB complex and its analytical application.
The fluorescence quenching of the Y-BPMPHD-CTMAB by nucleic acids is reported. It is considered that the Y-BPMPHD-CTMAB can form a large complex with nucleic acid through the electrostatic attraction in the pH range of 4.2-6.8. Under optimal conditions, the difference of fluorescence intensity between the system without and with nucleic acids is proportional to the concentration of nucleic acids over the range of 4.5 x 10(-8)-1.2 x 10(-5) g/mL for fsDNA and 3.2 x 10(-8)-3.0 x 10(-5) g/mL for yRNA, respectively. The detection limits are 14.0 ng/mL for fsDNA and 21.0 ng/mL for yRNA. The method is applied for the determination of nucleic acids in actual sample, and the result obtained is satisfactory.
Wu
Xia
X
Key Lab for Colloid & Interface Chemistry of Educational Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Sun
Shuna
S
Yang
Jinghe
J
Wang
Yuebo
Y
Li
Yuxiang
Y
Su
Benyu
B
D002021:Buffers; D002138:Calibration; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009696:Nucleic Acids / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
D002021:Buffers; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D013501:Surface-Active Agents