title: [Serum G-CSF levels in patients with chronic aplastic anemia].
abstract: OBJECTIVE
  To explore the pathogenesis of aplastic anemia.
  
  
  METHODS
  The spleen cells of BALB/c miceimmunized with rhG-CSF were fused with myeloma cell line SP2/0. Three murine hybridomas producing monoclonal antibody (McAb) against rhG-CSF were obtained. Serum levels of G-CSF in 54 cases of chronic aplastic anemia (CAA) were detected by ELISA method.
  
  
  RESULTS
  Serum G-CSF levels in 70.4% (38 of 54 cases) of the CAA patients were increased (272.76 +/- 58.39ng/L). There was a negative correlation between blood neutrophil counts and serum G-CSF levels (r = -0.535, P<0.01). CFU-GM were detected in 12 cases of CAA, and all was decreased (17.92 +/- 10.28/2 x 10(5) nucleated cell). Serum G-CSF levels in almost 30% (12 of 54 cases) of the CAA patients were not increased.
  
  
  CONCLUSIONS
  The hematopoietic progenitor cells of CAA patients were impaired, and some CAA could be treated with rhG-CSF and immunosuppressive agents.
authors.affiliation: Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: G
authors.initials: G
authors.lastname: Sun
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Shen
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D000741:Anemia, Aplastic / Q000097:blood*; D000818:Animals; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D016179:Granulocyte Colony-Stimulating Factor