title: Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry.
abstract: A crucial aim upon the completion of the human genome is the verification and functional annotation of all predicted genes and their protein products. Here we describe the mapping of peptides derived from accurate interpretations of protein tandem mass spectrometry (MS) data to eukaryotic genomes and the generation of an expandable resource for integration of data from many diverse proteomics experiments. Furthermore, we demonstrate that peptide identifications obtained from high-throughput proteomics can be integrated on a large scale with the human genome. This resource could serve as an expandable repository for MS-derived proteome information.
authors.affiliation: Nestlé Research Center, 1000 Lausanne 26, Switzerland. fdesiere@yahoo.com <fdesiere@yahoo.com>
authors.forename: Frank
authors.initials: F
authors.lastname: Desiere
authors.forename: Eric W
authors.initials: EW
authors.lastname: Deutsch
authors.forename: Alexey I
authors.initials: AI
authors.lastname: Nesvizhskii
authors.forename: Parag
authors.initials: P
authors.lastname: Mallick
authors.forename: Nichole L
authors.initials: NL
authors.lastname: King
authors.forename: Jimmy K
authors.initials: JK
authors.lastname: Eng
authors.forename: Alan
authors.initials: A
authors.lastname: Aderem
authors.forename: Rose
authors.initials: R
authors.lastname: Boyle
authors.forename: Erich
authors.initials: E
authors.lastname: Brunner
authors.forename: Samuel
authors.initials: S
authors.lastname: Donohoe
authors.forename: Nelson
authors.initials: N
authors.lastname: Fausto
authors.forename: Ernst
authors.initials: E
authors.lastname: Hafen
authors.forename: Lee
authors.initials: L
authors.lastname: Hood
authors.forename: Michael G
authors.initials: MG
authors.lastname: Katze
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Kennedy
authors.forename: Floyd
authors.initials: F
authors.lastname: Kregenow
authors.forename: Hookeun
authors.initials: H
authors.lastname: Lee
authors.forename: Biaoyang
authors.initials: B
authors.lastname: Lin
authors.forename: Dan
authors.initials: D
authors.lastname: Martin
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Ranish
authors.forename: David J
authors.initials: DJ
authors.lastname: Rawlings
authors.forename: Lawrence E
authors.initials: LE
authors.lastname: Samelson
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Shiio
authors.forename: Julian D
authors.initials: JD
authors.lastname: Watts
authors.forename: Bernd
authors.initials: B
authors.lastname: Wollscheid
authors.forename: Michael E
authors.initials: ME
authors.lastname: Wright
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Lihong
authors.initials: L
authors.lastname: Yang
authors.forename: Eugene C
authors.initials: EC
authors.lastname: Yi
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D030562:Databases, Protein; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D015894:Genome, Human; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000235:genetics*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012984:Software
chemical_list: D010455:Peptides; D020543:Proteome