title: Molecular tripods showing fluorescence enhancement upon binding to streptavidin.
abstract: We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Kim
authors.forename: Hey Young
authors.initials: HY
authors.lastname: Yoon
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Oh-Hoon
authors.initials: OH
authors.lastname: Kwon
authors.forename: Du-Jeon
authors.initials: DJ
authors.lastname: Jang
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Hong
mesh_terms: D001710:Biotin / Q000378:metabolism; D005453:Fluorescence; D011485:Protein Binding; D019809:Streptavidin / Q000378:metabolism*
chemical_list: D001710:Biotin; D019809:Streptavidin