title: Maturation by LctT is required for biosynthesis of full-length lantibiotic lacticin 481.
abstract: In lantibiotic lacticin 481 biosynthesis, LctT cleaves the precursor peptide and exports mature lantibiotic. Matrix-assisted laser desorption ionization-time of flight mass spectrometry revealed that a truncated form of lacticin 481 is produced in the absence of LctT or after cleavage site inactivation. Production of truncated lacticin 481 is 4-fold less efficient, and its specific activity is about 10-fold lower.
authors.affiliation: Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne Sud, BP 92116, 56321 Lorient Cedex, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Uguen
authors.forename: Thomas
authors.initials: T
authors.lastname: Hindré
authors.forename: Sandrine
authors.initials: S
authors.lastname: Didelot
authors.forename: Christel
authors.initials: C
authors.lastname: Marty
authors.forename: Dominique
authors.initials: D
authors.lastname: Haras
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Le Pennec
authors.forename: Karine
authors.initials: K
authors.lastname: Vallée-Réhel
authors.forename: Alain
authors.initials: A
authors.lastname: Dufour
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D004798:Enzymes; C481644:LctM protein, Lactococcus lactis; D011498:Protein Precursors; C082508:lacticin 481