Capillary electrophoretic and micellar electrokinetic separations of asymmetric dimethyl-L-arginine and structurally related amino acids: quantitation in human plasma.
We report the development of efficient electrophoretic methods for the separation and quantification of L-arginine and six naturally occurring derivatives that are structurally and functionally related. Capillary electrophoresis (CE) employing a concentrated borate buffer at pH 9.4 achieves the separation of mixtures containing dimethyl-L-arginine, NG-monomethyl-L-arginine, L-arginine, L-homoarginine, L-ornithine, and L-citrulline as 4-fluoro-7-nitrobenzofurazan derivatives. In addition, the separation of the isomeric dimethyl-L-arginine derivatives (symmetric and asymmetric) is attained with baseline resolution by micellar electrokinetic chromatography (MEKC) when a high concentration of deoxycholic acid is added as a surfactant to the same running buffer. The influence of buffer type, concentration, and pH on the separation was studied to optimize separation conditions. The limit of quantitation (LOQ) for asymmetric dimethyl-L-arginine in aqueous solution was determined to be 20 microM using UV absorption in a CE separation and 0.1 microM using laser induced fluorescence (LIF) detection in an MEKC separation. This newly developed method was successfully applied for the quantitation of asymmetric dimethyl-L-arginine and L-arginine in human plasma samples at levels that might be used as a clinical diagnostic for cardiovascular disease (0.125 microM LOQ).
Trapp
Gabriele
G
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Sydow
Karsten
K
Dulay
Maria T
MT
Chou
Tina
T
Cooke
John P
JP
Zare
Richard N
RN
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary; D019075:Electrophoresis, Capillary; D006801:Humans
C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; C018524:N,N-dimethylarginine; D001120:Arginine; D009327:4-Chloro-7-nitrobenzofurazan