A comparison of gas-liquid chromatography and a titration method for the determination of plasma non-esterified fatty add levels during pregnancy.
The level of plasma non-esterified fatty acids (NEFA) was measured by gas-liquid chromatography (GLC) and a titration method in 194 samples collected during pregnancy and from four days to 24 weeks post partum. Both techniques indicated a similar pattern of changes in plasma NEFA associated with pregnancy. The titration estimates of NEFA level were usually greater than those measured by GLC, and there was some suggestion that the disparity between the methods was increased at the end of pregnancy and was reduced at six weeks after delivery.
Young
M
M
M.R.C. Reproduction and Growth Unit, Princess Mary Maternity Hospital, Newcastle upon Tyne NE2 3RD.
Gilmore
E A
EA
McDonald-Gibson
R G
RG
Elliott
J A
JA
D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008137:Longitudinal Studies; D049590:Postpartum Period / Q000097:blood; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000097:blood; D011262:Pregnancy Trimester, Second / Q000097:blood; D011263:Pregnancy Trimester, Third / Q000097:blood; D017186:Titrimetry / Q000379:methods*
D005230:Fatty Acids, Nonesterified