[The recurrent nasal polyp analysed by mRNA difference to demonstrate].
OBJECTIVE
To look for the correlated gene of nasal polyp.


METHODS
The nasal polyp was analysed by mRNA difference to demonstrate, and the fragment of writing was analysed and appraised.


RESULTS
The amplification products of normal conchae tissue were compared with the nasal polyp tissue under the condition of background with one accord. The total 11 difference fragments were discovered during the 150-700 bp. One of the fragments (X-1) was completely homology with the section series of the eight chromosome long arm. There was a zine finger protein in the upper stream and the down stream. The fragment expressed in nasal polyp tissue.


CONCLUSIONS
Based on polymerase chain reaction (pcr), using differential display R. T. PCR (DD-RTPCR) technique, the difference express gene of nasal polyp could be effectively compared with normal nasal conchae tissue or cells, the correlated gene of nasal polyp tissue could screened out. The fragment (X-1) that was completely homology with the section series of the eight chromosome long arm may be a new identified gene which cause the nasal polyp.
Ni
Guansen
G
Department of Otorhinolaryngology, Shanghai Branch of First People's Hospital, Shanghai, 200081, China.
Chen
Wenwen
W
Zhu
Ying
Y
Zhao
Hanfang
H
D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger