[A method for preparation of genomic DNA from Grifola frondosa and construction of a genomic library].
Grifola frondosa, is a valuable medicinal fungus. High quality total genomic DNA is difficult to prepare due to its high polysaccharide content. A method for the preparation of Grifola frondosa total genomic DNA and construction of Grifola frondosa, genomic library is described. Genomic DNA prepared by this method is digested by Sau3A I restriction enzyme. Constructed genomic library give a titer of 2 x 10(5) transformants/50mg , with a average insert size of 14kb. This has paved way for the cloning of other Grifola frondosa genes and molecular biology studies.
Xu
Zhi-Xiang
ZX
Key Laboratory of Genetic Engineering, Ministry of Education/School of Life Sciences, Sun Yat-sen University, Guangzhou 510 275, China. xuzhixiang123@163.net
Cheng
Du
D
Li
Bao-Jian
BJ
D004271:DNA, Fungal / Q000302:isolation & purification*; D015698:Genomic Library; D048691:Grifola / Q000235:genetics*
D004271:DNA, Fungal