Protectivity of an immunoaffinity-purified 39 kDa capsular protein of avian Pasteurella multocida in mice.
A 39 kDa protein of avian Pasteurella multocida strain P-1059 (serovar A:3) was purified from a crude capsular extract by immunoaffinity chromatography by using a ligand of purified mouse monoclonal antibody to the 39 kDa capsular protein of the strain. Protective activity of the purified 39 kDa protein antigen was determined by inoculation of ddY mice twice with 25 or 125 mug of the protein and challenge-exposure with 10 or 50 LD(50) of strains P-1059 or X-73 (serovar A:1). The results showed that the antigen gave high protection (60 to 100%). These results indicated that the 39 kDa protein of avian P. multocida is a cross-protective antigen over serovars A:1 and A:3.
Ali
Hussam Al-Haj
HA
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Nippon Veterinary and Animal Science University, Musashino, Tokyo 180-8602, Japan.
Sawada
Takuo
T
Noda
Ken
K
D000818:Animals; D000911:Antibodies, Monoclonal; D016667:Bacterial Capsules / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002846:Chromatography, Affinity; D008024:Ligands; D051379:Mice / Q000276:immunology* / Q000382:microbiology; D010326:Pasteurella Infections / Q000517:prevention & control*; D016979:Pasteurella multocida / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D008024:Ligands