Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B.
Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-kappaB). The activation of NF-kappaB has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a transcriptional processivity and anti-termination factor, is sufficient to activate NF-kappaB in A549 cells. Electromobility shift assays show increased NF-kappaB complexes in the nuclei of M2-1-expressing cells. M2-1 protein is found in nuclei of M2-1-expressing cells and in RSV-infected cells. Co-immunoprecipitations of nuclear extracts of M2-1-expressing cells and of RSV-infected cells revealed an association of M2-1 with Rel A protein. Furthermore, the activation of NF-kappaB depends on the C-terminus of the RSV M2-1 protein, as shown by NF-kappaB-induced gene expression of a reporter gene construct.
Reimers
Kerstin
K
Klinik für Plastische, Hand-und Wiederherstellungschirurgie, Podbielskistrasse 380, D-30659 Hannover, Germany. reimers.kerstin@mh-hannover.de
Buchholz
Katja
K
Werchau
Hermann
H
D002460:Cell Line / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000494:pharmacology*
D016328:NF-kappa B; D014764:Viral Proteins