Antioxidative effect of kaempferol and its equimolar mixture with phenyltin compounds on UV-irradiated liposome membranes.
The work investigates the possibility of the protective action of kaempferol on phosphatidylcholine liposome membranes exposed to the pro-oxidative action of diphenyltin dichloride (DPhT) and triphenyltin chloride (TPhT) induced by UV radiation (lambda = 253.7 nm). The concentrations of kaempferol and its equimolar mixtures with DPhT and TPhT were determined so that they induce 50% inhibition in oxidation of liposomes irradiated with UV. They are 11.6, 10.0, and 4.5 microM/L, which constitute the following sequence of antioxidative activity: kaempferol/triphenyltin > kaempferol/diphenyltin > kaempferol. This relationship is confirmed by the results on the antiradical ability of kaempferol and its mixtures with DPhT and TPhT toward the free radical 2,2-diphenyl-1-picrylhydrazyl. Similar sequences obtained in both studies suggest a possible mechanism of the antiradical action of the mixtures as free radical scavengers. Kaempferol's ability, then documented, to form complexes with phenyltins indicates (a) a possible way to liquidate the peroxidation caused by the free radical forms of phenyltins and (b) the stabilizing role of chelating in the antioxidative action of the kaempferol/phenyltins. The differentiation in the action of the compounds studied may, among others, result from different localizations in the liposome membrane, which is indicated by the results of the fluorometric studies.
Gabrielska
Janina
J
Department of Physics and Biophysics, Agricultural University, Norwida 25, 50-375 Wrocław, Poland. jaga@ozi.ar.wroc.pl
Soczyńska-Kordala
Monika
M
Przestalski
Stanisław
S
D000975:Antioxidants / Q000494:pharmacology*; D004347:Drug Interactions; D044949:Kaempferols / Q000737:chemistry / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry* / Q000528:radiation effects; D009947:Organotin Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry; D014466:Ultraviolet Rays
D000975:Antioxidants; D044949:Kaempferols; D008081:Liposomes; D009947:Organotin Compounds; D010713:Phosphatidylcholines; C063613:phenyltin; C006552:kaempferol