title: Load independence of temperature-dependent Ca2+ recirculation fraction in canine heart.
abstract: Intramyocardial Ca(2+) recirculation fraction (RF) critically determines the economy of excitation-contraction coupling. RF is obtainable from the exponential decay of the postextrasystolic potentiation of left ventricular (LV) contractility. We have shown that RF remains unchanged despite increasing LV volume (LVV) at normothermia, but decreases with increasing temperature at a constant LVV. However, it remains unknown whether the temperature-dependent RF was not due to the simultaneously changed peak LV pressure (LVP) at a constant LVV. We hypothesized that this temperature-dependent RF would be independent of the simultaneous change in LVP. We used nine excised, cross-circulated canine hearts and allowed their LVs to contract isovolumically. During stable regular beats at 500 msec intervals, we inserted an extrasystolic beat at 360 msec interval followed by the postextrasystolic beats (PESs) at 500 msec intervals. We equalized the temperature-dependent peak LVPs of the regular beats at 36 degrees C and 38 degrees C to the peak LVP level of the stable regular beat at 33 degrees C by adjusting LVV. We fitted the same equation: nEmax = a.exp[-(i - 1)/tau(e)] + b.exp[-(i - 1)/tau(s)]cos[pi(i - 1)] + 1, used before to the normalized Emax (maximum elastance) values of PESi (i = 1-6) relative to the regular beat Emax. RF given by exp(-1/tau(e)) decreased by 19% to 38 degrees C from 33 degrees C. The temperature coefficient (Q(10)) of 1/RF was significantly greater than 1.3. The present results indicated a similar temperature dependence of RF and its Q(10) to those we observed previously without equalizing peak LVP. Thus, the temperature-dependent RF is independent of ventricular loading conditions.
authors.affiliation: Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan. mizuno-ju@excite.co.jp
authors.forename: J
authors.initials: J
authors.lastname: Mizuno
authors.forename: S
authors.initials: S
authors.lastname: Mohri
authors.forename: J
authors.initials: J
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Mikane
authors.forename: J
authors.initials: J
authors.lastname: Araki
authors.forename: T
authors.initials: T
authors.lastname: Nishiyama
authors.forename: K
authors.initials: K
authors.lastname: Hanaoka
authors.forename: F
authors.initials: F
authors.lastname: Kajiya
authors.forename: H
authors.initials: H
authors.lastname: Suga
mesh_terms: D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics*; D004285:Dogs; D009200:Myocardial Contraction; D012519:Sarcoplasmic Reticulum / Q000502:physiology; D015398:Signal Transduction; D013696:Temperature; D016276:Ventricular Function
chemical_list: D002118:Calcium