title: Chemoprevention against hepatocellular carcinoma of Cornus officinalis in vitro.
abstract: The water extracts of Cornus officinalis Sieb. et Zuce against hepatocellular carcinoma (HCC) was studied for its chemopreventive potential. Three HCC cell lines (HepG2, SK-Hep1 and PLC/PRF/5) and three leukemic cell lines (U937, K562 and Raji) were tested with XTT assay. Extracts of C. officinalis inhibited all these HCC cells and leukemic cells at a concentration of 100 microg/ml (P < 0.05) and was dose-dependent (P < 0.0001). P53 (P< 0.0001) and Ras (P = 0.001) significantly affected its activity against HCC. Extracts of C. officinalis also possessed the anti-oxidant activity through free radicals scavenging activity at a concentration of 50 microg/ml (P < 0.05). In summary, our experiment implied that C. officinalis might be a candidate for chemopreventive agent against HCC through the antioxidant and anti-neoplastic effects.
authors.affiliation: Graduate Institute of Medicine, College of Medicine, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Jung-San
authors.initials: JS
authors.lastname: Chang
authors.forename: Lien-Chai
authors.initials: LC
authors.lastname: Chiang
authors.forename: Fen-Fang
authors.initials: FF
authors.lastname: Hsu
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Lin
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D029768:Cornus; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D020298:U937 Cells; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D014969:Xanthine Oxidase