Revascularization of ischemic tissues by PDGF-CC via effects on endothelial cells and their progenitors.
The angiogenic mechanism and therapeutic potential of PDGF-CC, a recently discovered member of the VEGF/PDGF superfamily, remain incompletely characterized. Here we report that PDGF-CC mobilized endothelial progenitor cells in ischemic conditions; induced differentiation of bone marrow cells into ECs; and stimulated migration of ECs. Furthermore, PDGF-CC induced the differentiation of bone marrow cells into smooth muscle cells and stimulated their growth during vessel sprouting. Moreover, delivery of PDGF-CC enhanced postischemic revascularization of the heart and limb. Modulating the activity of PDGF-CC may provide novel opportunities for treating ischemic diseases.
Li
Xuri
X
The Center for Transgene Technology and Gene Therapy, Flanders Interuniversitary Institute for Biotechnology (VIB), University of Leuven, Leuven, Belgium.
Tjwa
Marc
M
Moons
Lieve
L
Fons
Pierre
P
Noel
Agnes
A
Ny
Annelii
A
Zhou
Jian Min
JM
Lennartsson
Johan
J
Li
Hong
H
Luttun
Aernout
A
Pontén
Annica
A
Devy
Laetitia
L
Bouché
Ann
A
Oh
Hideyasu
H
Manderveld
Ann
A
Blacher
Silvia
S
Communi
David
D
Savi
Pierre
P
Bono
Françoise
F
Dewerchin
Mieke
M
Foidart
Jean-Michel
JM
Autiero
Monica
M
Herbert
Jean-Marc
JM
Collen
D
D
Heldin
Carl-Henrik
CH
Eriksson
Ulf
U
Carmeliet
Peter
P
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D006614:Hindlimb / Q000098:blood supply / Q000187:drug effects; D006801:Humans; D007511:Ischemia / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D008222:Lymphokines; D051379:Mice; D008833:Microcirculation / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D019000:Phosphotyrosine / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; C411423:platelet-derived growth factor C; D019000:Phosphotyrosine; D017479:Receptors, Platelet-Derived Growth Factor