[Studies on the fingerprint and quantitative analyzes of quercetin from Aster tataricus].
OBJECTIVE
To establish a qualitative and quantitative method with RP-HPLC for controlling the quality of Aster tataricus L. f.


METHODS
The experimental conditions of the RP-HPLC method were as follows: Ultrasphere ODS C18 columns (250 mm x 4.6 mm, 5 microm), and the mobile phase methanol-acetonitrile (1:2) -0.4% phosphoric acid, the flow rate was 1.0 ml/min, the detection wave-lengths was at 360 nm and the column temperature was 25 degrees C. The qualitative fingerprint of the samples dealt by ultrasonic bath and the quantitative measurement of quercetin were finished under the selected chromatographic conditions.


RESULTS
Under the qualitative conditions, the 10 mutual peaks in RP-HPLC fingerprint of the samples from 6 different Aster tataricus L. f. can be used as index peaks for qualitative identification. In RP-HPLC quantitative analysis, the contents of quercetin in Aster tataricus L. f. were 0.135% approximately 0.246%.


CONCLUSIONS
The analytical method is simple, quick and accurate for controlling the quality of the medicinal material of Aster tataricus L. f.
Zhou
Junhui
J
Biology and Medicine Key Laboratory of Northwest University, Xi'an.
Wu
Weiping
W
Xie
Zhimin
Z
Sun
Wenji
W
D031173:Aster Plant / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D009716:Numerical Analysis, Computer-Assisted; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D011794:Quercetin / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D011794:Quercetin