title: Addition of recombinant human growth hormone to in vitro maturation medium of bovine oocytes.
abstract: The aim of this study was to increase the bovine embryonic development rate, adding recombinant human growth hormone (rhGH) to maturation medium of bovine oocytes. Oocytes were matured for 24 h in TCM 199 Earle's salts and five treatments were developed: T1, 0.01 IU/ml of recombinant human follicle stimulating hormone (rhFSH); T2, 0.01 IU/ml of rhFSH + 100 ng/ml of rhGH; T3, 0.01 IU/ml of rhFSH + 1000 ng/ml of rhGH; T4, 100 ng/ml of rhGH; and T5, 1000 ng/ml of rhGH at 39 degrees C and 5% of CO(2) in air and saturated humidity. In vitro fertilization from cumulus-oocyte complexes was conducted in TALP-Fert medium (18-22 h) and spermatozoa were selected by Percoll gradient. Zygotes were incubated in SOFaaci medium in 5% of CO(2) in air, 5% of O(2) at 39 degrees C and saturated humidity for 11 days. There was no statistical difference in cleavage rate and embryo production on day 7 and day 9 among treatments. However, the hatching rate increased significantly in the T4 and T5 treatments (11.0 and 12.8%, respectively), compared with the T1 treatment (4.6%) (p < 0.05). Therefore, the rhGH addition to the oocyte maturation medium showed beneficial effects on the hatching rate of in vitro-produced bovine embryos.
authors.affiliation: Department of Veterinarian Clinic and Surgery, Veterinary School of the University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: S E
authors.initials: SE
authors.lastname: Pozzobon
authors.forename: M A
authors.initials: MA
authors.lastname: Lagares
authors.forename: D S
authors.initials: DS
authors.lastname: Brum
authors.forename: F G
authors.initials: FG
authors.lastname: Leivas
authors.forename: M I B
authors.initials: MI
authors.lastname: Rubin
mesh_terms: D000818:Animals; D002417:Cattle; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D019382:Human Growth Hormone