Molecular analysis of S gene of spike glycoprotein of winter dysentery bovine coronavirus circulated in Korea during 2002-2003.
Since the molecular analysis of spike (S) glycoprotein gene of bovine coronavirus (BCoV) has been conducted and compared mainly among American and Canadian isolates and/or strains, it is unclear whether BCoV circulated in the other countries are distinctive in genetic characteristics. In the present study, we analyzed the S glycoprotein gene to characterize 10 winter dysentery (WD) coronavirus strains circulated in Korea during 2002-2003 and compared the nucleotide (nt) and deduced amino acid (aa) sequences with the other known BCoV. The phylogenetic analysis of the entire S glycoprotein gene revealed that the aa sequences of all Korean WD strains were more homologous to each other and were very closely related to respiratory bovine coronavirus (RBCV) strain OK and enteric bovine coronavirus (EBCV) strain LY-138, but were distinct from the other known BCoVs. Based on the phylogenetic analysis of the hypervariable region of the S1 subunit, all Korean WD strains clustered with the respiratory strain OK, BCQ3994 and the enteric strain LY-138, while the Canadian BCQ calf diarrhea and WD strains, and the American RBCV LSU, French EBCV F15 and avirulent VACC, L9, and Mebus strains clustered on a separate major branch. These data suggest that the WD strains circulated in Korea had a genetic property of both RBCV and EBCV and were significantly distinct from the ancestral enteric strain.
Jeong
Jae-Ho
JH
Laboratory of Animal Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Kim
Gye-Yeop
GY
Yoon
Soon-Seek
SS
Park
Su-Jin
SJ
Kim
You-Jung
YJ
Sung
Chang-Min
CM
Shin
Sung-Shik
SS
Lee
Bong-Joo
BJ
Kang
Mun-Il
MI
Park
Nam-Yong
NY
Koh
Hong-Bum
HB
Cho
Kyoung-Oh
KO
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D017124:Conserved Sequence; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017938:Coronavirus, Bovine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D019143:Evolution, Molecular; D007723:Korea; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics*
D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins