title: Lactone synthesis activity in a site-directed mutant of an extradiol catechol dioxygenase enzyme.
abstract: A H115Y site-directed mutant of extradiol catechol dioxygenase MhpB catalyses an intramolecular lactonisation reaction upon its natural substrate.
authors.affiliation: University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Sharon
authors.initials: S
authors.lastname: Mendel
authors.forename: Andrew
authors.initials: A
authors.lastname: Arndt
authors.forename: Timothy D H
authors.initials: TD
authors.lastname: Bugg
mesh_terms: D019943:Amino Acid Substitution; D002384:Catalysis; D049308:Dioxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007783:Lactones / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D007783:Lactones; D010105:Oxygenases; D049308:Dioxygenases; C083582:3-carboxyethylcatechol 2,3-dioxygenase; C439587:extradiol dioxygenase