Expression of SERCA2a is not regulated by calcineurin or upon mechanical unloading in skeletal muscle regeneration.
This study investigates to what extent the expression of the slow myosin heavy chain (MyHCI) isoform and the slow type sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) isoform are co-regulated in fibers of regenerating skeletal soleus muscle. Both overexpression of cain, a calcineurin inhibitor, or partial tenotomy prevented the expression of MyHCI but left SERCA2a expression unaffected in fibers of regenerating soleus muscles. These data complement those from different experimental models and clearly show that the expression of MyHCI and SERCA2a--the major proteins mediating, respectively, the slow type of contraction and relaxation--are not coregulated in regenerating soleus muscle.
Zádor
Erno
E
Institute of Biochemistry, Faculty of Medicine, Albert Szent-Györgyi Medical and Pharmaceutical Center, University of Szeged, BOBox 427, Dóm tér 9, H-6701 Szeged, Hungary. erno@biochem.szote.u-szeged.hu
Fenyvesi
Rita
R
Wuytack
Frank
F
D000818:Animals; D019703:Calcineurin / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D009126:Muscle Relaxation; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
D019703:Calcineurin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases