Induction of coronary arteritis with administration of CAWS (Candida albicans water-soluble fraction) depending on mouse strains.
The intraperitoneal administration of CAWS (water-soluble extracellular polysaccharide fraction obtained from the culture supernatant of Candida albicans) to mice induces coronaritis similar to Kawasaki disease. We analyzed differences in the production of cytokines involved in the occurrence of coronary arteritis among mouse strains, C3H/HeN, C57BL/6, DBA/2 and CBA/J that were injected with CAWS at 4 mg/mouse for 5 consecutive days in the first week and the fifth week of administration. The incidence of arteritis was 100% in C57BL/6, C3H/HeN and DBA/2 mice, but only 10% in CBA/J mice. The coronary arteritis observed in DBA/2 mice was the most serious, with several mice expiring during the observation period. The CAWS-sensitive strains revealed increased levels of IL-6 and IFN-gamma during the course of a specific response to CAWS by spleen cells. In contrast, IL-10 levels were observed to increase markedly in CAWS-resistant CBA/J mice, but not the CAWS-sensitive strains. However, TNF-alpha levels were more elevated only in DBA/2 mice. The difference in disease development and cytokine production strongly suggests that the genetic background of the immune response to CAWS contributes to the occurrence of coronary arteritis.
Nagi-Miura
Noriko
N
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.
Shingo
Yuko
Y
Adachi
Yoshiyuki
Y
Ishida-Okawara
Akiko
A
Oharaseki
Toshiaki
T
Takahashi
Kei
K
Naoe
Shiro
S
Suzuki
Kazuo
K
Ohno
Naohito
N
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001167:Arteritis / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D002176:Candida albicans / Q000737:chemistry* / Q000276:immunology; D003331:Coronary Vessels / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011134:Polysaccharides / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity*; D013045:Species Specificity
D016207:Cytokines; D011134:Polysaccharides