title: Effects of complete Freund's adjuvant on immunohistochemical distribution of IL-1beta and IL-1R I in neurons and glia cells of dorsal root ganglion.
abstract: OBJECTIVE
  To investigate the effects of complete Freund adjuvant (CFA) on inflammatory hyperalgesia and morphological change of the coexistence of interleukin-1 beta (IL-1beta) and type I IL-1 receptor (IL-1RI) in neurons and glia cells of rat dorsal root ganglion (DRG).
  
  
  METHODS
  The pain-related parameters and the expression of IL-1RI and IL-1beta positive neurons and glia cells of DRG in normal saline (NS) and adjuvant-induced arthritic (AA) group were examined with pain behavior assessment methods and immunohistochemical assay, respectively.
  
  
  RESULTS
  Five hours, 1 d, and 2 d after intra-articular injection of 50 microL CFA, tactile hyperalgesia induced by CFA was observed in the foot flexion and extension scores of the ipsilateral hindpaw of AA group. Three days after injection, the distribution of IL-1RI/IL-1beta double-stained coexisted neurons and glia cells were observed in ipsilateral DRG of both groups. The number of IL-1beta positive neurons, IL-1RI positive neurons, IL-1beta/IL-1RI double-stained neurons, and IL-1RI positive glia cells in ipsilateral DRG of the AA group were higher than that of NS group (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  The coexistence of IL-1beta and IL-1RI in neurons and nonneuronal cells suggests an as yet unknown autocrine and/or paracrine function of IL-1beta in the DRG. The function was enhanced in articular arthritis induced by CFA and could play an important role in hyperalgesia under inflammatory conditions.
authors.affiliation: Department of Neurobiology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.forename: Jun-Rui
authors.initials: JR
authors.lastname: Tang
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Ai
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wang
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: Cao
authors.forename: Ling-Li
authors.initials: LL
authors.lastname: Li
authors.forename: Chuan-You
authors.initials: CY
authors.lastname: Lin
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Guan
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology; D005620:Freund's Adjuvant; D005727:Ganglia, Spinal / Q000473:pathology*; D006930:Hyperalgesia / Q000139:chemically induced / Q000473:pathology*; D007375:Interleukin-1 / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D053573:Receptors, Interleukin-1 Type I
chemical_list: D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D005620:Freund's Adjuvant