Phenol-containing saline solution as a diluent for adenosine 5'-monophosphate in bronchial challenge testing.
OBJECTIVE
To investigate the effect of dissolving adenosine 5'-monophosphate (AMP) with phenol-containing saline solution on the stability and the bronchoconstrictive properties of this indirect agonist.


METHODS
Eleven subjects with asthma well controlled with short-acting inhaled beta2-agonists as required or with inhaled corticosteroids were studied. Bronchial challenge tests with AMP dissolved with either normal saline solution or saline solution containing 0.4% phenol were performed on separate days. Furthermore, to assess the potential influence of the phenol-containing saline solution on the stability of the bronchoconstrictor agent, AMP solutions in concentrations of 40 microg/mL and 400 microg/mL were prepared in saline solution and phenol-containing saline solution and, after 30 min, the AMP levels were determined by high-performance liquid chromatography (HPLC) assay.


RESULTS
The geometric mean AMP provocative concentration causing a 20% fall in FEV1 (PC20) was 13.49 mg/mL (95% confidence interval [CI], 6.76 to 26.91) for the saline solution method, and AMP PC20 for the saline solution with phenol method was 8.91 mg/mL (95% CI, 3.39 to 23.44) [p = 0.18]. No significant differences were found between the concentrations of AMP made in saline solution compared to those made in phenol-containing saline solution measured by HPLC.


CONCLUSIONS
These observations indicate that normal saline solution with or without phenol can be used as the diluent for AMP. However, since a potential risk with AMP of industrial sources is the bacterial contamination, adding a preservative such as phenol to a saline solution diluent might be recommended.
Prieto
Luis
L
Sección de Alergología, Hospital Universitario Dr Peset, Valencia, Spain. prieto_jes@gva.es
Badiola
Carlos
C
Cortijo
Julio
J
Pérez-Francés
Carmen
C
Gutiérrez
Valentina
V
Lanuza
Amparo
A
D000249:Adenosine Monophosphate / Q000008:administration & dosage* / Q000737:chemistry; D000280:Administration, Inhalation; D000328:Adult; D001249:Asthma / Q000175:diagnosis*; D001985:Bronchial Provocation Tests; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010636:Phenols / Q000032:analysis; D012965:Sodium Chloride / Q000737:chemistry
D010636:Phenols; D000249:Adenosine Monophosphate; D012965:Sodium Chloride