title: Activation of STAT3 signaling in human stomach adenocarcinoma drug-resistant cell line and its relationship with expression of vascular endothelial growth factor.
abstract: OBJECTIVE
  To investigate the difference in activation of STAT3 signaling between two human stomach adenocarcinoma cell lines: 5-fluorouracil resistant cell line and its parental cell line, and to evaluate its relationship with the expression of vascular endothelial growth factor (VEGF).
  
  
  METHODS
  Western blot and electrophoretic mobility shift assay (EMSA) were used to detect the expression of phospho-STAT3 protein and constitutive activation of STAT3 in two human stomach adenocarcinoma cell lines, 5-fluorouracil resistant cell line SGC7901/R and its parental cell line SGC7901, respectively. The mRNA expression of VEGF was analysed by semi-quantitative RT-PCR. The expressive intensity of VEGF protein was measured by immunocytochemistry.
  
  
  RESULTS
  The expressions of phospho-STAT3 protein and constitutive activation of STAT3 between two human stomach adenocarcinoma cell lines were different. Compared with the parental cell line SGC7901, the STAT3-DNA binding activity and the expressive intensity of phospho-STAT3 protein were lower in the drug-resistant cell line SGC7901/R. The expression levels of VEGF mRNA and its encoded protein were also decreased in drug-resistant cell line.
  
  
  CONCLUSIONS
  Over-expression of VEGF may be correlated with elevated STAT3 activation in parental cell line. Lower VEGF expression may be correlated with decreased STAT3 activation in resistant cell line, which may have resulted from negative feedback regulation of STAT signaling.
authors.affiliation: Department of Gastroenterology, Ruijin Hospital of Shanghai Second Medical University, 197 Ruijin er Road, Shanghai 200025, China. graceyu1028@sohu.com
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Min-Min
authors.initials: MM
authors.lastname: Qiao
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Yun-Lin
authors.initials: YL
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma; D015153:Blotting, Western; D045744:Cell Line, Tumor / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm / Q000502:physiology; D025461:Feedback, Physiological; D006801:Humans; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D050796:STAT3 Transcription Factor; D015398:Signal Transduction / Q000502:physiology; D013274:Stomach Neoplasms; D015534:Trans-Activators / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators; D042461:Vascular Endothelial Growth Factor A; D004247:DNA