An on-line technique for monitoring propionic acid fermentation.
An on-line technique, based on measuring the increase in pressure due to CO(2) release in a closed air-tight reactor, was used to evaluate the fermentation of lactate by propionibacteria. The method was applied to batch cultures of Propionibacterium shermanii grown in yeast extract/sodium lactate medium containing lactate as a carbon source under micro-aerophilic conditions. Gas pressure evolution was compared both with substrate consumption and metabolites production and with acidification and growth. Linear relationships were found between gas pressure variation, lactate consumption and propionate and acetate production. The technique also enabled the evaluation of total CO(2) produced, by taking account of pressure, oxygen and pH measurements. These results tend to show that this simple and rapid method could be useful to monitor propionic acid bacteria growth.
Benjelloun
Houda
H
Laboratoire Génie des Procédés Industriels UMR CNRS 6067, Département Génie Chimique, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, France.
Rochex
Alice
A
Lecouturier
Didier
D
Dechemi
Siham
S
Lebeault
Jean-Michel
JM
D000085:Acetates / Q000378:metabolism; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D019344:Lactic Acid / Q000378:metabolism; D008365:Manometry / Q000379:methods; D009862:Online Systems; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D011312:Pressure; D011422:Propionates / Q000378:metabolism*; D011424:Propionibacterium / Q000254:growth & development / Q000378:metabolism
D000085:Acetates; D011422:Propionates; D002245:Carbon Dioxide; D019344:Lactic Acid; C029658:propionic acid; D010100:Oxygen