title: Proteomics of immune-challenged Drosophila melanogaster larvae hemolymph.
abstract: In the last decade, the fruit fly Drosophila melanogaster has emerged as a promising invertebrate model for the investigation of innate immunity, in part because of its well characterised genetics. The information provided by the innumerous reports on Drosophila's immune response indicates that a large number of genes, in addition to the well-known antimicrobial peptide genes, are both up- and down-regulated upon immune challenge. Nevertheless, their contribution to fighting off infection has not been seriously addressed. With the application of recent advances in proteomics, the effects of an immune challenge in the overall modification of Drosophila 2-DE protein patterns were investigated. The aim of this study was to investigate hemolymph proteins differentially expressed between control and immunised larvae sets, which could be related solely to the Drosophila immune response. The list of immune-related protein spots included heat shock proteins and other proteins with chaperone properties, serine proteases, phenol oxidase, and Drosophila antioxidant system components, which accounted for 21% of the total of 70 identified proteins, metabolic enzymes implicated in pathways such as cellular respiration, fatty-acid oxidation, protein biosynthesis, and structural proteins.
authors.affiliation: Department of Chemistry, University of Aveiro, Portugal.
authors.forename: Sofia
authors.initials: S
authors.lastname: de Morais Guedes
authors.forename: Rui
authors.initials: R
authors.lastname: Vitorino
authors.forename: Rosário
authors.initials: R
authors.lastname: Domingues
authors.forename: Kenneth
authors.initials: K
authors.lastname: Tomer
authors.forename: A J Ferrer
authors.initials: AJ
authors.lastname: Correia
authors.forename: Francisco
authors.initials: F
authors.lastname: Amado
authors.forename: Pedro
authors.initials: P
authors.lastname: Domingues
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000276:immunology; D029721:Drosophila Proteins / Q000276:immunology* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000276:immunology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000276:immunology; D006458:Hemolymph / Q000276:immunology* / Q000378:metabolism*; D007113:Immunity, Innate / Q000276:immunology; D007814:Larva / Q000276:immunology / Q000378:metabolism; D020543:Proteome / Q000276:immunology / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D029721:Drosophila Proteins; D020543:Proteome