title: Phenotypic characterization and distribution of dendritic cells in parotid gland tumors.
abstract: Evidence on the relationship between the infiltration of dendritic cells (DCs) and prognosis in head and neck tumors exists. Interestingly, only limited information is available regarding the maturation state and distribution of DCs in parotid gland tumors. The purpose of our study was therefore to extend these observations and to investigate in more detail the density and distribution of mature DCs and Langerhans cells (LCs) in parotid gland tumors. We present immunohistochemical evidence of characterization and distribution of DCs and LCs in parotid gland tumors, enclosed pleomorphic adenomas and malignant parotid tumors. Two populations of mature DCs could be identified, P55(+)-DCs and DC-LAMP(+)-DCs, whereas LCs could be identified as Langerin(+)-LCs. The overall impression was that parotid gland tumors contained only few mature DCs and LCs. Considering the sparsity of mature DCs in the malignant tissues, anti-tumor response can be only limited. On the basis of our data, we imply that the application of DC vaccination in combination with other modalities for treatment of parotid gland carcinoma should be taken into account. In this regard, the utilization of DC immunotherapy for management of minimal residual disease after resection of primary tumor can be promising. Putative targets expressed in this type of tumors have to be defined.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital, Innsbruck, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Wischatta
authors.forename: Thomas
authors.initials: T
authors.lastname: Brunhuber
authors.forename: Andreas
authors.initials: A
authors.lastname: Neher
authors.forename: Laco
authors.initials: L
authors.lastname: Kacani
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Romani
authors.forename: Annelies
authors.initials: A
authors.lastname: Schrott-Fischer
authors.forename: Georg Mathias
authors.initials: GM
authors.lastname: Sprinzl
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000032:analysis; D051907:Lysosomal Membrane Proteins; D008297:Male; D037241:Mannose-Binding Lectins / Q000032:analysis; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000276:immunology / Q000473:pathology*; D010641:Phenotype; D011379:Prognosis
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C121916:CD207 protein, human; D006684:HLA-DR Antigens; D037181:Lectins, C-Type; D051907:Lysosomal Membrane Proteins; D037241:Mannose-Binding Lectins