title: Ultrastructural defects of acrosome in infertile men.
abstract: To study the ultrastructural changes of acrosomes in fertile and infertile males and to identify the morphological alterations closely related to male infertility, forty spermatozoa from 8 fertile males and 60 spermatozoa from 12 infertile males were collected and observed by scanning electron microscope. There were significant changes in the morphology of the circular sulcus, acrosome membrane and head of the infertile sperm. The circular sulcus of spermatozoa was more serrated (39/60 vs 2/40) and blurred (40/60 vs 4/40) in the infertile group than in the fertile group. The acrosome of infertile group occupied a bigger proportion of sperm head (70+/-8% vs 57+/-6%) than in the fertile group. The acrosome membrane in the infertile group was less intact (18/60 vs 31/40) and less smooth (19/60 vs 32/40) than in the fertile group. More droplets were attached to the acrosome membrane in the infertile (39/60 vs 11/40) than in the fertile groups. The abnormality in acrosome ultrastructure seems to be significantly higher in infertile than in fertile males, suggesting a potential role of such defects for male fertility defect.
authors.affiliation: Department of Urology, The Affiliated Hospital of Jiaotong University, Shanghai Sixth People's Hospital, Shanghai, China. yujj917@163.com
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Yu
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Xu
mesh_terms: D000177:Acrosome / Q000648:ultrastructure*; D000328:Adult; D002470:Cell Survival; D006801:Humans; D007248:Infertility, Male / Q000473:pathology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology