Marinomonas pontica sp. nov., isolated from the Black Sea.
A Gram-negative, polarly flagellated bacterium was isolated from a sea-water sample collected from the Karadag Natural Reserve of the Eastern Crimea and characterized to clarify its taxonomic position. 16S rRNA gene sequence-based phylogenetic analysis of this novel organism revealed Marinomonas vaga, Marinomonas communis, Marinomonas mediterranea, Marinomonas primoryensis and 'Marinomonas protea' as its closest relatives (similarity 95-97 %). The G+C content of the DNA was 46.5 mol%. The organism grew between 4 and 33 degrees C, tolerated 10 % NaCl, was slightly alkaliphilic and was not able to degrade starch, gelatin, agar or Tween 80. Phosphatidylethanolamine (53.4 %) and phosphatidylglycerol (46.6 %) were the predominant phospholipids. The major fatty acids were 16 : 0 (15.5 %), 16 : 1omega7 (26.7 %) and 18 : 1omega7 (47.1 %). The phylogenetic, genetic and physiological properties of the organism placed it within a novel species, proposed as Marinomonas pontica sp. nov., the type strain of which is 46-16T (=LMG 22531T=KMM 3492T=UCM 11075T).
Ivanova
Elena P
EP
Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia.
Onyshchenko
Olga M
OM
Institute of Microbiology and Virology, Ukraine Academy of Sciences, Ukraine.
Christen
Richard
R
UMR6543 CNRS - Université de Nice Sophia Antipolis, Centre de Biochimie, Parc Valrose, F06108 Nice Cedex 2, France.
Lysenko
Anatoly M
AM
Institute of Microbiology of the Russian Academy of Sciences, 117811 Moscow, Russian Federation.
Zhukova
Natalia V
NV
Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041, Palchevskogo Street 17, Vladivostok, Russian Federation.
Shevchenko
Ludmila S
LS
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
Kiprianova
Elena A
EA
Institute of Microbiology and Virology, Ukraine Academy of Sciences, Ukraine.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D044130:Oceanospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014455:Ukraine
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S