Involvement of acetosyringone in plant-pathogen recognition.
In this study, acetosyringone was identified as one of the major extracellular phenolics in tobacco suspension cells and was shown to have bioactive properties that influence early events in plant-bacterial pathogenesis. In our model system, tobacco cell suspensions treated with bacterial isolate Pseudomonas syringae WT (HR+) undergo a resistant interaction characterized by a burst in oxygen uptake several hours after inoculation. When the extracellular concentration of acetosyringone in tobacco cell suspensions was supplemented with exogenous acetosyringone, the burst in oxygen uptake occurred as much as 1.5h earlier. The exogenous acetosyringone had no effect on tobacco suspensions undergoing susceptible interactions with Pseudomonas tabaci or a non-resistant interaction with a near-isogenic mutant derivative of isolate P. syringae WT (HR+). Resistant interactions with isolate P. syringae WT (HR+) also produce an oxidative burst which oxidizes the extracellular acetosyringone. This study demonstrates that acetosyringone, and likely other extracellular phenolics, may have bioactive characteristics that can influence plant-bacterial pathogenesis.
Baker
C Jacyn
CJ
Molecular Plant Pathology Laboratory, ARS, USDA, Beltsville, MD 20705, USA. bakerc@ba.ars.usda.gov
Mock
Norton M
NM
Whitaker
Bruce D
BD
Roberts
Daniel P
DP
Rice
Clifford P
CP
Deahl
Kenneth L
KL
Aver'yanov
Andrey A
AA
D000098:Acetophenones / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D011549:Pseudomonas / Q000472:pathogenicity*; D044224:Pseudomonas syringae / Q000472:pathogenicity; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology*; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*
D000098:Acetophenones; C051667:acetosyringone; D010100:Oxygen