title: Immunisation with virion-loaded plasmacytoid or myeloid dendritic cells induces primary Th-1 immune responses.
abstract: Dendritic cells (DCs) induce different types of immune responses depending on their lineage and activation signals. When exposed to inactivated pseudorabiesvirus (iPRV), plasmacytoid but not myeloid DCs released IFN-alpha and IL-12. Remarkably, both iPRV-pulsed DC types were able to induce primary IFN-gamma producing T cells and IgG isotype switching in vivo. In contrast, tetanus toxoid pulsed DCs did not induce detectable primary immune responses. The efficacy of antiviral T and B cell priming proved dependent on the recipient's genotype. We conclude that either plasmacytoid or myeloid DCs pulsed with inactivated virus suffice to induce primary Th1-polarised immune responses.
authors.affiliation: Virology Unit, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, 3584 CL Utrecht, The Netherlands.
authors.forename: Marel C de
authors.initials: MC
authors.lastname: Wit
authors.forename: Marian C
authors.initials: MC
authors.lastname: Horzinek
authors.forename: Bart L
authors.initials: BL
authors.lastname: Haagmans
authors.forename: Virgil E J C
authors.initials: VE
authors.lastname: Schijns
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis; D016898:Interferon-alpha / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D015848:Interleukin-5; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D014771:Virion / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D016898:Interferon-alpha; D015848:Interleukin-5; D013745:Tetanus Toxoid; D014765:Viral Vaccines; D018664:Interleukin-12; D007371:Interferon-gamma