Down-regulation of the kps region 1 capsular assembly operon following attachment of Escherichia coli type 1 fimbriae to D-mannose receptors.
A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to d-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli.
Schwan
William R
WR
Department of Microbiology, University of Wisconsin-La Crosse, 1725 State Street, La Crosse, WI 54601, USA. schwan.will@uwlax.edu
Beck
Michael T
MT
Hultgren
Scott J
SJ
Pinkner
Jerry
J
Woolever
Nathan L
NL
Larson
Thomas
T
D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology; D016667:Bacterial Capsules / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D017930:Genes, Reporter; D037181:Lectins, C-Type / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D033903:Periplasmic Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic / Q000502:physiology; D001743:Urinary Bladder / Q000382:microbiology / Q000502:physiology
D029968:Escherichia coli Proteins; C076835:FtsZ84 protein, E coli; C088016:KpsD protein, E coli; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D033903:Periplasmic Proteins; D011956:Receptors, Cell Surface