title: Measuring forces between protein fibers by microscopy.
abstract: We propose a general scheme for measuring the attraction between mechanically frustrated semiflexible fibers by measuring their thermal fluctuations and shape. We apply this analysis to a system of sickle hemoglobin (HbS) fibers that laterally attract one another. These fibers appear to "zip" together before reaching mechanical equilibrium due to the existence of cross-links into a dilute fiber network. We are also able to estimate the rigidities of the fibers. These rigidities are found to be consistent with sickle hemoglobin "single" fibers 20 nm in diameter, despite recent experiments indicating that fiber bundling sometimes occurs. Our estimate of the magnitude of the interfiber attraction for HbS fibers is in the range 8 +/- 7 kBT/microm, or 4 +/- 3 k(B)T/microm if the fibers are assumed, a priori to be single fibers (such an assumption is fully consistent with the data). This value is sufficient to bind the fibers, overcoming entropic effects, although extremely chemically weak. Our results are compared to models for the interfiber attraction that include depletion and van der Waals forces. This technique should also facilitate a similar analysis of other filamentous protein assembles in the future, including beta-amyloid, actin, and tubulin.
authors.affiliation: Department of Physics, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Jones
authors.forename: J C
authors.initials: JC
authors.lastname: Wang
authors.forename: R W
authors.initials: RW
authors.lastname: Briehl
authors.forename: M S
authors.initials: MS
authors.lastname: Turner
mesh_terms: D001703:Biophysics / Q000379:methods*; D002845:Chromatography; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004227:Dithionite / Q000737:chemistry; D006451:Hemoglobin, Sickle / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances / Q000737:chemistry; D008857:Microscopy, Interference; D008954:Models, Biological; D015233:Models, Statistical; D011485:Protein Binding; D011487:Protein Conformation; D013696:Temperature; D013816:Thermodynamics
chemical_list: D003432:Cross-Linking Reagents; D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D046911:Macromolecular Substances; D004227:Dithionite