Biomedical applications of fluorescence imaging in vivo.
Optical imaging can advance knowledge of cellular biology and disease at the molecular level in vitro and, more recently, in vivo. In vivo optical imaging has enabled real-time study to track cell movement, cell growth, and even some cell functions. Thus, it can be used in intact animals for disease detection, screening, diagnosis, drug development, and treatment evaluation. This review includes a brief introduction to fluorescence imaging, fluorescent probes, imaging devices, and in vivo applications in animal models. It also describes a quantitative fluorescence detection method with a reconstruction algorithm for determining the location of fluorophores in tissue and addresses future applications of in vivo fluorescence imaging.
Hassan
Moinuddin
M
Laboratory of Integrative and Medical Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Building 9 Room B1E11, Bethesda, Maryland 20892, USA.
Klaunberg
Brenda A
BA
D000465:Algorithms; D000818:Animals; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D005453:Fluorescence; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted; D051379:Mice; D023421:Models, Animal; D009374:Neoplasms, Experimental / Q000473:pathology; D051381:Rats
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins