title: A novel family of glucosyl 1,5-anhydro-d-fructose derivatives synthesised by transglucosylation with dextransucrase from Leuconostoc mesenteroides NRRL B-512F.
abstract: 1,5-Anhydro-d-fructose (AF), a metabolite of starch/glycogen degradation, is a good antioxidant. With the prospect of increasing its applications and use as a food ingredient, AF glucosylation catalysed by the dextransucrase from Leuconostoc mesenteroides NRRL B-512F was performed in the presence of sucrose. This led to AF glucosylated derivatives containing alpha-(1-->6) linkages named 1,5-anhydro-d-fructo-glucooligosaccharides (AFGOS). LC-MS analyses showed that AFGOS with a degree of polymerisation (DP) of up to 7 were synthesised. The amount of AFGOS produced and the average DP increased by using a high sucrose/AF molar ratio and high total sugar concentration. AFGOS were proved to present antioxidant properties quite similar to AF.
authors.affiliation: Laboratoire de Biotechnologie-Bioprocédés UMR CNRS 5504, UMR INRA 792, INSA DGBA 135 avenue de Rangueil 31077 Toulouse Cedex 04, France.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Richard
authors.forename: Shukun
authors.initials: S
authors.lastname: Yu
authors.forename: Pierre
authors.initials: P
authors.lastname: Monsan
authors.forename: Magali
authors.initials: M
authors.lastname: Remaud-Simeon
authors.forename: Sandrine
authors.initials: S
authors.lastname: Morel
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D004187:Disaccharides / Q000138:chemical synthesis*; D005947:Glucose / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry*; D007934:Leuconostoc / Q000201:enzymology*
chemical_list: C488015:1,4-anhydro-3-O-glucopyranosylfructose; D000975:Antioxidants; D004187:Disaccharides; D005964:Glucosyltransferases; C020025:dextransucrase; D005947:Glucose