title: LexA analog (dra0074) is a regulatory protein that is irrelevant to recA induction.
abstract: The protein DRA0074 is suggested to be another LexA in Deinococcus radiodurans, having similar motifs and RecA-mediated cleavage activity to D. radiodurans LexA (dra0344). However, its function has not been studied. We disrupted the gene dra0074 and measured its effect on RecA induction using fusion translation, immunoblot, and proteomic analysis. Results showed that the product of gene dra0074 is not involved in RecA induction, but is a regulator of other metabolisms in D. radiodurans.
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, 310029, Hangzhou, China.
authors.forename: Duohong
authors.initials: D
authors.lastname: Sheng
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zheng
authors.forename: Bing
authors.initials: B
authors.lastname: Tian
authors.forename: Binghui
authors.initials: B
authors.lastname: Shen
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Hua
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D034301:Deinococcus / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D015151:Immunoblotting; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D040901:Proteomics; D011938:Rec A Recombinases / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein