Prevalence of human herpesvirus-8 infection in HIV-positive patients with and without Kaposi's sarcoma in Hungary.
Human herpesvirus-8 (HHV-8) infection of 130 Hungarian HIV-positive individuals with or without Kaposi's sarcoma was investigated from 158 serum and 122 peripheral blood samples using anti-latency-associated nuclear antigen (LANA) indirect immunofluorescence assay (IFA), recombinant orf65 and orfK8.1 antigen enzyme-linked immunosorbent assays (ELISAs), Western blot assays and orf26 specific nested polymerase chain reaction (PCR). The overall prevalence of HHV-8 infection was found to be 31.5% (41/130) among the Hungarian HIV-positive patients. This seroprevalence rate is 7-11-fold higher than that of healthy HIV-negative blood donors in Hungary. The highest prevalence of HHV-8 infection (36.1%, 35/97) was observed in homo- or bisexual patients. Similar to the serologic results, HHV-8 DNA was not always detectable in all serial samples previously shown to be positive for HHV-8 DNA.
Szalai
Erika
E
Department of Microbiology, St. László Hospital, Gyáli u. 5-7, 1097 Budapest, Hungary. erszalai@freemail.hu
Gerlei
Zsuzsanna
Z
Szlávik
János
J
Szládek
Györgyi
G
Patel
Ravi
R
Hunyadi
János
J
Gergely
Lajos
L
Juhász
Attila
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005814:Genes, Viral; D015658:HIV Infections / Q000150:complications*; D006566:Herpesviridae Infections / Q000150:complications* / Q000453:epidemiology*; D019288:Herpesvirus 8, Human / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000276:immunology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011994:Recombinant Proteins; D012307:Risk Factors; D012514:Sarcoma, Kaposi / Q000453:epidemiology*; D012725:Sexual Behavior; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D009687:Nuclear Proteins; C119019:ORF65 protein, human herpesvirus 8; D011994:Recombinant Proteins; D014764:Viral Proteins; C109540:latency-associated nuclear antigen