title: Vaccine-induced protection against Borna disease in wild-type and perforin-deficient mice.
abstract: Borna disease virus (BDV) can persistently infect the central nervous system and induce CD8+ T-cell-mediated neurological disease in MRL mice. To determine whether specific immune priming would prevent disease, a prime-boost immunization protocol was established in which intramuscular injection of a recombinant parapoxvirus expressing BDV nucleoprotein (BDV-N) was followed by intraperitoneal infection with vaccinia virus expressing BDV-N. Immunized wild-type and perforin-deficient mice remained healthy after intracerebral infection with BDV and contained almost no virus in the brain at 5 weeks post-challenge. Immunization failed to induce resistance against BDV in mice lacking mature CD8+ T cells. Immunization of perforin-deficient mice with a poxvirus vector expressing mutant BDV-N lacking the known CD8+ T-cell epitope did not efficiently block multiplication of BDV in the brain and did not prevent neurological disease, indicating that vaccine-induced immunity to BDV in wild-type and perforin-deficient mice resulted from the action of CD8+ T cells.
authors.affiliation: Department of Virology, University of Freiburg, D-79104 Freiburg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hausmann
authors.affiliation: Department of Virology, University of Freiburg, D-79104 Freiburg, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Baur
authors.affiliation: Department of Virology, University of Freiburg, D-79104 Freiburg, Germany.
authors.forename: Karin R
authors.initials: KR
authors.lastname: Engelhardt
authors.affiliation: Federal Research Center for Virus Diseases of Animals, Institute for Immunology, D-72076 Tuebingen, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Fischer
authors.affiliation: Federal Research Center for Virus Diseases of Animals, Institute for Immunology, D-72076 Tuebingen, Germany.
authors.forename: Hanns-Joachim
authors.initials: HJ
authors.lastname: Rziha
authors.affiliation: Department of Virology, University of Freiburg, D-79104 Freiburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Staeheli
mesh_terms: D000818:Animals; D001890:Borna Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D001891:Borna disease virus / Q000235:genetics / Q000276:immunology; D001921:Brain / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors; D007273:Injections, Intramuscular; D008562:Membrane Glycoproteins / Q000172:deficiency / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D009698:Nucleoproteins / Q000096:biosynthesis / Q000235:genetics; D018157:Parapoxvirus / Q000235:genetics; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D018984:Epitopes, T-Lymphocyte; D008562:Membrane Glycoproteins; D009698:Nucleoproteins; D052899:Pore Forming Cytotoxic Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D054353:Perforin