Lack of EGF receptor contributes to drug sensitivity of human germline cells.
Germline mutations have been associated with generation of various types of tumour. In this study, we investigated genetic alteration of germline tumours that affect the drug sensitivity of cells. Although all germline tumour cells we tested were hypersensitive to DNA-damaging drugs, no significant alteration was observed in their DNA repair activity or the expression of DNA repair proteins. In contrast, germline tumours expressed very low level of epidermal growth factor receptor (EGFR) compared to drug-resistant ovarian cancer cells. An immunohistochemical analysis indicated that most of the primary germline tumours we tested expressed very low level of EGFR. In accordance with this, overexpression of EGFR in germline tumour cells showed an increase in drug resistance, suggesting that a lack of EGFR, at least in part, contributes to the drug sensitivity of germline tumours.
Park
S-J
SJ
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA. slee@iupui.edu
Armstrong
S
S
Kim
C-H
CH
Yu
M
M
Robertson
K
K
Kelley
M R
MR
Lee
S-H
SH
D015153:Blotting, Western; D004260:DNA Repair / Q000502:physiology; D045643:DNA Repair Enzymes / Q000096:biosynthesis; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D066246:ErbB Receptors / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009373:Neoplasms, Germ Cell and Embryonal / Q000188:drug therapy / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics; D014407:Tumor Cells, Cultured
D066246:ErbB Receptors; D045643:DNA Repair Enzymes