In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.
The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.
Supatto
Willy
W
Mechanics and Genetics of Developmental Embryology, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168, Curie Institute, 11 Rue Pierre et Marie Curie, F-75005 Paris, France.
Débarre
Delphine
D
Moulia
Bruno
B
Brouzés
Eric
E
Martin
Jean-Louis
JL
Farge
Emmanuel
E
Beaurepaire
Emmanuel
E
D000818:Animals; D002465:Cell Movement; D003599:Cytoskeleton / Q000502:physiology; D004331:Drosophila melanogaster / Q000196:embryology*; D047108:Embryonic Development; D007834:Lasers; D018613:Microscopy, Confocal; D009024:Morphogenesis