The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes.
We describe developmentally regulated genes in two strains of Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria; of these, about 60 were novel. Only two genes -- encoding HSP83 and an importin-related protein -- appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level.
Brems
Stefanie
S
DKFZ, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany.
Guilbride
D Lys
DL
Gundlesdodjir-Planck
Drifa
D
Busold
Christian
C
Luu
Van-Duc
VD
Schanne
Michaela
M
Hoheisel
Jörg
J
Clayton
Christine
C
D000818:Animals; D001769:Blood / Q000469:parasitology; D018507:Gene Expression Regulation, Developmental; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D020543:Proteome; D015800:Protozoan Proteins; D012333:RNA, Messenger