Efficient secretion of human lysozyme from the yeast, Kluyveromyces lactis.
Efficient secretion of human lysozyme from the yeast, Kluyveromyces lactis, was achieved by using more stable vectors in the order of S11 replication origin-containing episomal vector < full-length K. lactis plasmid pKD1-containing vector < centromeric vector < chromosome-integrated vectors. Cells containing a PGK (phosphoglycerate kinase) promoter-driven integration vector grown in non-selective rich medium achieved the highest level of secretion, approximately 100 microg lysozyme secretion ml(-1) culture: this level was approximately 10-fold higher than that achieved by episomal vectors. An additional copy of the protein disulfide isomerase gene further facilitated the secretion.
Iwata
Takako
T
Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Tanaka
Ryoichi
R
Suetsugu
Masae
M
Ishibashi
Matsujiro
M
Tokunaga
Hiroko
H
Kikuchi
Masakazu
M
Tokunaga
Masao
M
D024861:Genetic Enhancement / Q000379:methods; D006801:Humans; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics*; D009113:Muramidase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D014162:Transfection / Q000379:methods*
D011994:Recombinant Proteins; D009113:Muramidase