title: Rapid binding of concanavalin A and maltose-polyrotaxane conjugates due to mobile motion of alpha-cyclodextrins threaded onto a poly(ethylene glycol).
abstract: Maltose-polyrotaxane conjugates (Mal-PRXs), in which maltose-conjugated alpha-cyclodextrins (alpha-CDs) are threaded onto a poly(ethylene glycol) (PEG) chain capped with benzyloxycarbonyl-L-tyrosine, were characterized in terms of their molecular motion and the relation to multivalent interactions between the maltose moiety and concanavalin A from Canavalia ensiformis (Con A). Spin-lattice relaxation time (T1) and spin-spin relaxation time (T2) of alpha-CD C(1), maltosyl C(1), and PEG methylene protons in the Mal-PRXs revealed that the mobile motion of alpha-CDs in the polyrotaxane governed the molecular motion of maltosyl groups in alpha-CDs and threading PEG chain. The association constant (Ka) of the Mal-PRXs with 22, 38 and 53% of alpha-CD threading was 5.7 x 10(4), 1.1 x 10(6), and 5.3 x 10(5) (M(-1)-maltose), respectively. The largest Ka value of the Mal-PRX with 38% of alpha-CD threading was well correlated with the T1 and T2 values of maltosyl groups and alpha-CD, suggesting that the mobile motion of maltose-conjugated alpha-CDs in the Mal-PRX contributes to the highest affinity with Con A. Initial rate of binding with Con A was also governed by the mobile motion of maltose-conjugated alpha-CDs. Therefore, we concluded that both highly molecular motion due to the mobile motion of maltose-conjugated alpha-CDs and multivalency of the Mal-PRXs contributes to inducing rapid Con A binding.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatunokuchi, Ishikawa 923-1292, Japan.
authors.forename: Tooru
authors.initials: T
authors.lastname: Ooya
authors.forename: Hideto
authors.initials: H
authors.lastname: Utsunomiya
authors.forename: Masaru
authors.initials: M
authors.lastname: Eguchi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yui
mesh_terms: D003208:Concanavalin A / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D007700:Kinetics; D008320:Maltose / Q000737:chemistry*; D008956:Models, Chemical; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D043862:Rotaxanes / Q000737:chemistry*; D014443:Tyrosine / Q000031:analogs & derivatives; D047391:alpha-Cyclodextrins / Q000737:chemistry*
chemical_list: D003505:Cyclodextrins; D004337:Drug Carriers; D043862:Rotaxanes; D047391:alpha-Cyclodextrins; C475673:polyrotaxane; D020442:Poloxamer; D003208:Concanavalin A; D011092:Polyethylene Glycols; D014443:Tyrosine; D008320:Maltose