Determination of picroside II in dog plasma by HPLC and its application in a pharmacokinetics study.
A sensitive and simple high-performance liquid chromatography method with UV detection was developed and validated for determining picroside II in dog plasma. Paeoniflorin was employed as internal standard and the sample pre-treatment procedure consists of deproteinization by addition of acetonitrile. Chromatographic separations were performed on a Shimadzu VP-ODS column (250 x 4.6 mm i.d., 5 microm). The mobile phase consisted of acetonitrile-0.1% acetic acid aqueous (v/v), 23:77, v/v, at a rate of 1 mL/min. Detection was carried out at a wavelength of 266 nm. Calibration standards ranged from 0.25 to 500 microg/mL in dog plasma and the mean correlation coefficient of 0.9981 was found for the linear calibration curves (n = 6). The limit of quantification (LOQ) was 0.25 microg/mL. Intra- and inter-assay RSD ranged from 0.70 to 7.5%. Accuracy (%bias) ranged from -6.3 to 6.0%. This method was applied to the pharmacokinetic study of picroside II in dogs. The study demonstrated the plasma picroside II concentration-time curves were fitted to the two-compartment open model and showed linear pharmacokinetics.
Yang
Fu-Chuan
FC
Institute of Medicinal Plants Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100094, People's Republic of China.
Yang
Shi-Lin
SL
Xu
Li-Zhen
LZ
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000097:blood* / Q000493:pharmacokinetics*; D004285:Dogs; D004355:Drug Stability; D005615:Freezing; D005960:Glucosides / Q000097:blood* / Q000493:pharmacokinetics*; D057888:Iridoid Glucosides; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002934:Cinnamates; D005960:Glucosides; D057888:Iridoid Glucosides; C416837:picroside II