Cytochrome C release induces apoptosis of nasopharyngeal carcinoma (NPC) by antitumor glyfoline.
The mechanism of action, leakage of cytochrome c from mitochondria into cytosol, for the antineoplastic compound glyfoline was examined. Additionally, our current studies revealed that glyfoline induced apoptotic changes and arrested cell cycle procession at the G2/M phase in nasopharyngeal carcinoma (NPC). A reverse transcriptase polymerase chain reaction (RT-PCR) showed no specific changes of apoptosis-related gene expression (i. e., bax, ICE-alpha,beta, bcl-2, and c-myc). However, no similar changes were detected in fibroblasts and peripheral lymphocytes after glyfoline treatment suggesting that glyfoline has a higher affinity for tumor cells than for normal cells.
Su
Tsann-Long
TL
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. tlsu@ibms.sinica.edu.tw
Huang
Hwei-Ming
HM
Wang
Chung-Kwe
CK
Wu
Han-Chung
HC
Lin
Chin-Tarng
CT
D000166:Acridines / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor / Q000187:drug effects / Q000378:metabolism; D045304:Cytochromes c / Q000187:drug effects* / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020128:Inhibitory Concentration 50; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027821:Rutaceae
D000166:Acridines; D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D010936:Plant Extracts; C075802:glyfoline; D045304:Cytochromes c