Light-induced wilting and its molecular mechanism in epicotyls of dark-germinated pea (Pisum sativum L.) seedlings.
Possible mechanisms behind the light-induced wilting of dark-germinated pea (Pisum sativum L.) epicotyls were studied. Illumination with photosynthetically active radiation caused a fast turgor loss and wilting in the middle segments of the epicotyls accompanied by accumulation of water in the intercellular cavities. During this process, room temperature fluorescence emission spectra showed gradual bleaching of porphyrin-type pigments, which was lessened by incubating the epicotyls with excess ascorbate before illumination. Detection of singlet oxygen and lipid peroxidation products in the illuminated epicotyls suggested the occurrence of porphyrin-photosenzitized membrane damage as a cause of disordered water status and sequential wilting.
Erdei
Noémi
N
Department of Plant Anatomy Eötvös University Pázmámy P. s. 1/c H-1117 Budapest, Hungary.
Barta
Csengele
C
Hideg
Eva
E
Böddi
Béla
B
D018525:Germination; D008027:Light; D015227:Lipid Peroxidation / Q000528:radiation effects; D018532:Pisum sativum / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D018462:Photobiology; D011166:Porphyrins / Q000378:metabolism / Q000528:radiation effects; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D026082:Singlet Oxygen / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014867:Water / Q000378:metabolism
D011166:Porphyrins; D014867:Water; D026082:Singlet Oxygen