Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo.
Embryonic stem cells (ES cells), bone marrow-derived mesenchymal stem cells, umbilical cord blood-derived mesenchymal stem cells, and hepatic stem cells in liver have been known as a useful source that can induce to differentiate into hepatocytes. In this study, we examined whether human adipose tissue-derived stromal cells (hADSC) can differentiate into hepatic lineage in vitro. hADSC, that were induced to differentiate into hepatocyte-like cells by the treatment of HGF and OSM, had morphology similar to hepatocytes. Addition of DMSO enhanced differentiation into hepatocytes. RT-PCR and immunocytochemical analysis showed that hADSC express albumin and alpha-fetoprotein during differentiation. Differentiated hADSC showed LDL uptake and production of urea. Additionally, transplanted hADSC to CCl4-injured SCID mouse model were able to be differentiated into hepatocytes and they expressed albumin in vivo. Mesenchymal stem cells isolated from human adipose tissue are immunocompatible and are easily isolated. Therefore, hADSC may become an alternative source to hepatocyte regeneration or liver cell transplantation.
Seo
Min Jeong
MJ
Department of Physiology, College of Medicine, Pusan National University, Pusan 602-739, Republic of Korea.
Suh
Su Young
SY
Bae
Yong Chan
YC
Jung
Jin Sup
JS
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism* / Q000637:transplantation; D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D048429:Cell Size / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008875:Middle Aged; D053683:Oncostatin M; D010455:Peptides / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014508:Urea / Q000378:metabolism
D008077:Lipoproteins, LDL; C508351:OSM protein, human; C508352:Osm protein, mouse; D010455:Peptides; D053683:Oncostatin M; D017228:Hepatocyte Growth Factor; D014508:Urea; D004121:Dimethyl Sulfoxide