title: Stimulation of mycelia growth in several mushroom species by rice husks.
abstract: When supplemented to the culture medium of mushroom Coprinus cinereus, rice husks soaked beforehand in methanol stimulated mycelia growth up to a concentration of 80 mg/ml dose-dependently, whereas the non-treated stimulated mycelia growth up to 20 mg/ml. This result suggests the existence of both stimulatory and inhibitory substances in rice husks. Since momilactone A (MLA) is recognized as one of the phytoalexins in rice husks, its biological activity against mycelia growth was tested. Momilactone A inhibited mycelia growth at 5 microg/disc, whereas the methanol extract of husks did so at 1 mg/disc, wherein 0.2 microg of MLA was estimated by LC/MS/MS. Thus the phytoalexins including MLA should inhibit mycelia growth. Rice husks stimulated mycelia growth in some edible mushroom species such as Grifola frondosa (maitake), Lentinus edodes (shiitake), Pleurotus eryngii (eringi), and P. ostreatus (hiratake). Our findings might lead to the development of new profitable cultivation methods for mushrooms using rice husks.
authors.affiliation: Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan. hanai@akita-pu.ac.jp
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Hanai
authors.forename: Sayuri
authors.initials: S
authors.lastname: Ishida
authors.forename: Chiaki
authors.initials: C
authors.lastname: Saito
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Maita
authors.forename: Miyako
authors.initials: M
authors.lastname: Kusano
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tamogami
authors.forename: Masana
authors.initials: M
authors.lastname: Noma
mesh_terms: D000363:Agaricales / Q000254:growth & development*; D000383:Agriculture / Q000379:methods; D025282:Mycelium / Q000254:growth & development*; D012275:Oryza / Q000737:chemistry*; D013997:Time Factors