Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant huang-qin (Scutellaria baicalensis Georgi) using high-performance liquid chromatography with diode array and mass spectrometric detection.
A method of analysis of eight flavones using high-performance liquid chromatography (HPLC)-diode array detection (DAD)-mass spectrometry (MS) in root and aerial tissues of the medicinal plant Scutellaria baicalensis was developed. The identity of the analytes was confirmed using retention time, UV-vis and mass spectral comparisons to commercial standards. Both UV-vis and mass spectral patterns were characterized for glycosylated flavones. Two additional flavone glycosides were tentatively identified as chrysin-7-glucuronide and wogonoside, but not quantified. Greenhouse and in vitro-grown tissues were analyzed with flavone concentrations ranges of 0.14-150 and 0.030-1.7 microg/mg for greenhouse root and shoot tissue, respectively, and 0.0068-6.4 and 0.082-1.5 microg/mg for in vitro-grown roots and shoots, respectively.
Horvath
Christopher R
CR
Department of Plant Agriculture, University of Guelph, Guelph, Ont., Canada.
Martos
Perry A
PA
Saxena
Praveen K
PK
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047309:Flavones / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry*; D018520:Plant Shoots / Q000737:chemistry*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D047309:Flavones