Use of epichlorohydrin-treated chitosan resin as an adsorbent to isolate kappa-casein glycomacropeptide from sweet whey.
This study was undertaken to develop a method to isolate glycomacropeptide (GMP), a bioactive compound, from sweet whey by using chitosan resins as anion exchangers. Shrimp shells were used to prepare two chitosan (polyglucosamine) resins, one with the primary amine (-NH(2)) (resin A) and the other with the secondary amine (-NH-) (resin B) as the major functional group. These resins were tested as adsorbents for the isolation of GMP from sweet whey, and the results obtained were compared with those obtained with commercial anion exchangers. The most important finding in this experiment was that the GMP binding capacity of resin A was much higher than that of resin B. Resin A may be the anion exchanger to be tested for industrial scale production of GMP. Amino acid analysis of the GMP-depleted whey fraction suggests that this product can replace sweet whey as an ingredient in various food products including infant formulas, bakery products, and beverages.
Nakano
Takuo
T
Alberta Dairy Association Research Unit, Department of Agricultural Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. tnakano@ualberta.ca
Ikawa
Noriaki
N
Ozimek
Lech
L
D000327:Adsorption; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000837:Anion Exchange Resins / Q000737:chemistry; D002364:Caseins / Q000302:isolation & purification*; D048271:Chitosan / Q000737:chemistry*; D004811:Epichlorohydrin / Q000494:pharmacology*; D006020:Glycopeptides / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D000067816:Whey Proteins
D000596:Amino Acids; D000837:Anion Exchange Resins; D002364:Caseins; D006020:Glycopeptides; D008894:Milk Proteins; D012117:Resins, Synthetic; D000067816:Whey Proteins; C020251:kappa-casein glycomacropeptide; D004811:Epichlorohydrin; D048271:Chitosan