title: [Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion].
abstract: OBJECTIVE
  To study effect of triptolide (TL) on neuronal apoptosis in cerebral tissue of rat after ischemia-reperfusion.
  
  
  METHODS
  Triptolide at dose 0.2 or 0.4 mg x kg(-1) was intraperitoneally injected once a day for 4 d. The focal cerebral ischemia-reperfusion model was established with thread embolism in middle artery before triptolide injection on the fourth day. Neurological deficit score of rats was evaluated; and immunohistochemical techniques were used to count positive cells of express of MPO and TUNEL in cerebraltissue.
  
  
  RESULTS
  Compared with the control group, the deficit of neural function was significantly improved, and the number of infiltrate of neutrophil and neuronal apoptosis in cerebral tissue was remarkably reduced in two TL-treated groups.
  
  
  CONCLUSIONS
  The results suggested that TL can inhibit infiltration of neutrophil and decrease the degree of neuronal apoptosis in cerebral tissue.
authors.affiliation: Medical School of Ningbo University, Ningbo 315211, China. weidengmin99@sohu.com
authors.forename: Deng-ming
authors.initials: DM
authors.lastname: Wei
authors.forename: Guang-zhao
authors.initials: GZ
authors.lastname: Huang
authors.forename: Yi-gu
authors.initials: YG
authors.lastname: Zhang
authors.forename: Guang-xun
authors.initials: GX
authors.lastname: Rao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002545:Brain Ischemia / Q000473:pathology; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004852:Epoxy Compounds; D005260:Female; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020556:Neutrophil Infiltration / Q000187:drug effects; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*; D029757:Tripterygium / Q000737:chemistry
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D018696:Neuroprotective Agents; D010616:Phenanthrenes; C001899:triptolide