title: Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum erythrocyte binding ligand 1 (EBL-1)-derived peptides.
abstract: Erythrocyte binding ligand 1 (EBL-1) is a member of the ebl multigene family involved in Plasmodium falciparum invasion of erythrocytes. We found that five EBL-1 high-activity binding peptides (HABPs) bound specifically to erythrocytes: 29895 ((41)HKKKSGELNNNKSGILRSTY(60)), 29903 ((201)LYECGK-KIKEMKWICTDNQF(220)), 29923 ((601)CNAILGSYADIGDIVRGLDV(620)), 29924((621)WRDINTNKLSEK-FQKIFMGGY(640)), and 30018 ((2481)LEDIINLSKKKKKSINDTSFY(2500)). We also show that binding was saturable, not sialic acid-dependent, and that all peptides specifically bound to a 36-kDa protein on the erythrocyte membrane. The five HABPs inhibited in vitro merozoite invasion depending on the peptide concentration used, suggesting their possible role in the invasion process.
authors.affiliation: Fundación Instituto de Inmunología de Colombia, Carrera 50 No. 26-00, Bogotá, Colombia. hernando_curtidor@fidic.org.co
authors.forename: Hernando
authors.initials: H
authors.lastname: Curtidor
authors.forename: Luis E
authors.initials: LE
authors.lastname: Rodríguez
authors.forename: Marisol
authors.initials: M
authors.lastname: Ocampo
authors.forename: Ramses
authors.initials: R
authors.lastname: López
authors.forename: Javier E
authors.initials: JE
authors.lastname: García
authors.forename: John
authors.initials: J
authors.lastname: Valbuena
authors.forename: Ricardo
authors.initials: R
authors.lastname: Vera
authors.forename: Alvaro
authors.initials: A
authors.lastname: Puentes
authors.forename: Magnolia
authors.initials: M
authors.lastname: Vanegas
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry*; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010963:Plasmodium falciparum / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry*
chemical_list: D000953:Antigens, Protozoan; D003432:Cross-Linking Reagents; C066001:Duffy antigen binding protein, Plasmodium; D008024:Ligands; D010455:Peptides; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D019158:N-Acetylneuraminic Acid