[Determination of orientin and vitexin in Trollius chinesis preparation by HPLC].
OBJECTIVE
To establish a HPLC method for determining orientin and vitexin in Trollius chinesis preparation.


METHODS
The separation was carried out on Hypersil ODS column with a mixture of acetonitrile-1% acetic acid solution (15:85) as the mobile phase, at a flow rate of 1.0 mL x min(-1). The detection wavelength was set 340 nm.


RESULTS
Orientin showed a good linear relationship in the range of 8.42-84.2 microg x mL(-1) (r = 0.9999) and the average recovery is 98.3%, RSD is 0.8%. Vitexin showed a good linear relationship in the range of 8.07-80.7 microg x mL(-1) (r = 0.9999) and the average recovery is 102.4%. RSD is 1.7%.


CONCLUSIONS
The method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Trollius chinesis preparation.
Liu
Zhi
Z
Guizhou-Eakan Pharmaceutical Co., Ltd., Guiyang 550001, China.
Wang
Li
L
Li
Wen
W
Huang
Yong
Y
Xu
Zu-chao
ZC
D047310:Apigenin / Q000032:analysis*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D029626:Ranunculaceae / Q000737:chemistry*; D013607:Tablets
D002214:Capsules; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D005960:Glucosides; D013607:Tablets; D047310:Apigenin; C032731:vitexin; C065886:orientin