title: Use of a Beet necrotic yellow vein virus RNA-5-derived replicon as a new tool for gene expression.
abstract: A new gene-expression system based on RNA-5 of Beet necrotic yellow vein virus (BNYVV) was constructed to allow the expression of recombinant proteins in virally infected cells. Replication and expression levels of the RNA-5-based replicon containing the green fluorescence protein (GFP) gene were compared with those obtained with the well-characterized RNA-3-derived replicon (Rep-3). When RNA-3 and/or RNA-4 BNYVV RNAs were added to the inoculum, the expression levels of RNA-5-encoded GFP were considerably reduced. To a lesser extent, RNA-3-derived GFP expression was also affected by the presence of RNA-4 and -5. Both RNA-3- and RNA-5-derived molecules were able to express proteins within the same infected cells. Together with Rep-3, the RNA-5-derived replicon thus provides a new tool for the co-expression of different recombinant proteins. In Beta macrocarpa, Rep-5-GFP was able to move in systemic tissues in the presence of RNA-3 and thus provides a new expression system that is not restricted to the inoculated leaves.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Schmidlin
authors.affiliation: Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Link
authors.affiliation: Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Mutterer
authors.affiliation: Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Guilley
authors.affiliation: Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France.
authors.forename: David
authors.initials: D
authors.lastname: Gilmer
mesh_terms: D017790:Closterovirus / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D018515:Plant Leaves / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012093:Replicon
chemical_list: D012367:RNA, Viral; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins