[Evaluation of anti-HCV detection kits using recombinant antigens derived from various HCV regions].
OBJECTIVE
To evaluate the first and second assay kits currently used in blood centers for screening HCV infected blood, and to provide basis for a better match of the two assay kits.


METHODS
Using the newly developed multi-recombinant-HCV-antigen supplementary assay kit, the authors evaluated concurrently the specificity and sensitivity of two domestic and one imported anti-HCV detection kits.


RESULTS
Discrepancy in specificity and sensitivity existed among the two domestic HCV kits, and overall quality was slightly below that of leading or main stream imported HCV kit.


CONCLUSIONS
The newly developed multi-recombinant-HCV-antigen supplementary assay kit is useful in the evaluation of HCV antibody detection kit currently in use. It provides qualified assessing kit to capture antibodies against various HCV antigens. The present paper provided guidance for selecting a better match of the two screening kits and improved screening efficiency.
Deng
Ping
P
Changchun Municipal Blood Center, Changchun 130031, China.
Zhang
Hui-jie
HJ
Li
Yan
Y
Liu
Wei
W
Wang
Qiu-ping
QP
Chu
Ji-hui
JH
Zhang
He-qui
HQ
D001782:Blood Donors; D005069:Evaluation Studies as Topic; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood* / Q000276:immunology; D018936:Hepatitis C Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity
D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D011933:Reagent Kits, Diagnostic