MIR spectroscopic analysis on sugar metabolic and ethanol productive kinetics of suspension TBY-2 and rice cells pre-cultured in various media.
The influence of sugars in pre-cultivation media suspended plant cells on the kinetics of the sugar uptake and the ethanol production was studied by mid-infrared spectroscopy using a Fourier transform infrared spectrometer (FT-IR) equipped with an attenuate total reflection accessory (ATR). We performed the plant cell cultivation with Nicotiana tabacum cv. Bright Yellow No.2 (TBY-2) cells and Oryza sativa L., Japonica, cv. Nipponbare (rice) cells, respectively, in pre-culture and culture media, which had various types of glucose, fructose, sucrose or glucose-fructose mixtures. The results confirmed the kinetic differences between the TBY-2 cells and rice cells. These results suggested that the TBY-2 cells consumed sugar before growth and the rice cells consumed sugar after growth, moreover, the ethanol content increased just after cell growth was activated based on the non-dimensional cultivation time for the cell growth behavior.
Yamanaka
Atsushi
A
Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, Tsu, Mie 514-8507, Japan.
Hashimoto
Atsushi
A
Kanou
Mikihito
M
Kameoka
Takaharu
T
D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D003470:Culture Media / Q000032:analysis / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D012275:Oryza / Q000378:metabolism*; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014026:Nicotiana / Q000378:metabolism*
D002241:Carbohydrates; D003470:Culture Media; D000431:Ethanol