title: Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity.
abstract: Doxorubicin is an important and effective anticancer drug widely used for the treatment of various types of cancer but its clinical use is limited by dose-dependent cardiotoxicity. Elevated tissue levels of cellular superoxide anion/oxidative stress are a mechanism by which doxorubicin-induced cardiotoxicity. Selected medicinal plant extracts were tested for their antioxidant capacity and cardioprotective effect against doxorubicin-induced cardiotoxicity. The cardiac myoblasts H9c2 were incubated with the antioxidants ascorbic acid, trolox, N-acetylcysteine or selected medicinal plant extracts including; 1) ethanolic extracts from Curcuma longa L-EtOH Phyllanthus emblica L-EtOH, and Piper rostratum Roxb-EtOH; and 2) water extracts from Curcuma longa L-H2O and Morus alba L-H2O. The cardioprotective effects of these extracts were evaluated by crystal violet cytotoxicity assay. IC50s of doxorubicin were compared in the presence or absence of ascorbic acids, trolox, N-acetylcysteine or plant extracts. Morus alba L-H2O showed the highest antioxidant properties evaluated by ferric reducing/antioxidant power assay. Ascorbic acid and N-acetylcysteine had modest effects on the protection of doxorubicin-induced cytotoxicity while trolox showed insignificant protective effect. All plant extracts protected cardiac toxicity at different degrees except that Curcuma longa L-EtOH had no protective effect. Phyllanthus emblica-EtOH (100 microg/ml) showed the highest cardioprotective effect (approximately 12-fold doxorubicin IC50 increase). The data demonstrate that antioxidants from natural sources may be useful in the protection of cardiotoxicity in patients who receive doxorubicin.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. suvara@swu.ac.th
authors.forename: Suvara K
authors.initials: SK
authors.lastname: Wattanapitayakul
authors.forename: Linda
authors.initials: L
authors.lastname: Chularojmontri
authors.forename: Angkana
authors.initials: A
authors.lastname: Herunsalee
authors.forename: Suphan
authors.initials: S
authors.lastname: Charuchongkolwongse
authors.forename: Somchit
authors.initials: S
authors.lastname: Niumsakul
authors.forename: John A
authors.initials: JA
authors.lastname: Bauer
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D004353:Drug Evaluation, Preclinical; D005290:Ferric Compounds / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D032383:Myocytes, Cardiac / Q000187:drug effects; D010084:Oxidation-Reduction; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats
chemical_list: D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D002316:Cardiotonic Agents; D005290:Ferric Compounds; D004317:Doxorubicin