Eco-epidemiological survey of Leishmania (Viannia) braziliensis American cutaneous and mucocutaneous leishmaniasis in Ribeira Valley River, Paraná State, Brazil.
Leishmaniasis is endemic since last century in Adrianópolis Municipality, Ribeira Valley and is a serious public health. A study carried out during 1993-2003 on epidemiological surveys conducted in rural communities showed 339 new cases of cutaneous leishmaniasis (CL) detected from four municipalities (Adrianópolis, Cerro Azul, Doutor Ulysses and Rio Branco do Sul). A larger prevalence of cutaneous lesions was observed in rural workers (36%), women with domestic activities (18%), and younger students (31%). Multiple lesions were noticed in 53% of patients, but only one case of mucocutaneous leishmaniasis was reported. Twenty stocks were isolated from patients with characteristics lesions and were identified as Leishmania (Viannia) braziliensis using multi-locus enzyme electrophoresis (MLEE) and Random Amplified DNA (RAPD). In Phlebotominae survey, five species were obtained. Lutzomyia intermedia sl. represented 97.5% in peridomiciliar area and 100% in domicile. A canine serological survey made (Indirect Immunofluorescence Antibody Test, IFAT and Enzyme Linked Immunosorbent Assay, ELISA) in six rural county of Adrianópolis Municipality during 1998-1999 showed that 15.1% (24/159) of dogs were sera reactive. No lesions were observed in dogs and no parasite was isolated from lymph node aspirates and biopsies. In wild reservoirs study, only seven animals (Cricetidae, Desmodus sp. and edentates) were captured, but no parasites were found in culture from deep organs. The paper presents results of our 10 years study on cutaneous leishmaniasis survey in the Ribeira River Valley, East Region of Paraná State, Brazil. Environment changes in this region are also discussed.
de Castro
Edilene Alcântara
EA
Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Patologia Básica, Centro Politécnico, Jardim das Américas, 81.531-990, Curitiba, Paraná, Brazil.
Luz
Ennio
E
Telles
Flávio Queiroz
FQ
Pandey
Ashok
A
Biseto
Alceu
A
Dinaiski
Marlene
M
Sbalqueiro
Ives
I
Soccol
Vanete Thomaz
VT
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D004197:Disease Reservoirs; D004285:Dogs / Q000469:parasitology; D004592:Electrophoresis, Starch Gel; D005260:Female; D006736:Horses / Q000469:parasitology; D006801:Humans; D007892:Leishmania braziliensis / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D011576:Psychodidae; D019105:Random Amplified Polymorphic DNA Technique; D012424:Rural Population; D012621:Seasons; D016036:Seroepidemiologic Studies; D012882:Skin Tests