PP2A-dependent transactivation of the cyclin A promoter by SV40 ST is mediated by a cell cycle-regulated E2F site.
The Simian Virus 40 (SV40) small-t antigen (ST) plays an important role in driving cell proliferation, enhancing transformation by the large-T (LT) antigen. Potential targets of ST are the cyclin kinase inhibitor p27 and the cyclin A gene itself. Transactivation of the cyclin A promoter by ST depends on the interaction of ST with protein phosphatase 2A (PP2A) and occurs through a cell cycle-regulated E2F site near the transcription start site of the promoter. A third SV40 early protein, 17KT, also transactivates the cyclin A promoter but, in this case, transactivation depends on the dnaJ domain of the protein.
Skoczylas
Christine
C
Department of Microbiology-Immunology, Northwestern University, 303 E. Chicago Avenue, Chicago, IL 60611, USA.
Henglein
Berthold
B
Rundell
Kathleen
K
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D019925:Cyclin A / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050684:E2F Transcription Factors; D006801:Humans; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D054648:Protein Phosphatase 2; D013539:Simian virus 40 / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
D000957:Antigens, Viral, Tumor; D018797:Cell Cycle Proteins; D019925:Cyclin A; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D014157:Transcription Factors; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2