title: Human monoclonal antibodies that neutralize anthrax toxin by inhibiting heptamer assembly.
abstract: A panel of human anti-anthrax protective antigen IgG1 monoclonal antibodies were evaluated to determine the mechanism of toxin neutralization. AVP-22G12, AVP-1C6 and AVP-21D9 bound to the protective antigen with picomolar affinities to distinct non-overlapping linear epitopes. Two of the antibodies neutralized the anthrax toxin by completely inhibiting the protective antigen oligomer assembly process in vitro.
authors.affiliation: Avanir Pharmaceuticals, Antibody Technology, 11388 Sorrento Valley Rd, San Diego, CA 92121, USA. fwang@avanir.com
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Paul
authors.initials: P
authors.lastname: Ruther
authors.forename: Ivy
authors.initials: I
authors.lastname: Jiang
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Sawada-Hirai
authors.forename: Shu Man
authors.initials: SM
authors.lastname: Sun
authors.forename: Rebecca
authors.initials: R
authors.lastname: Nedellec
authors.forename: Phillip R
authors.initials: PR
authors.lastname: Morrow
authors.forename: Angray S
authors.initials: AS
authors.lastname: Kang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D061067:Antibodies, Monoclonal, Humanized; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D000080908:Broadly Neutralizing Antibodies; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009500:Neutralization Tests
chemical_list: C000601130:AVP-21D9; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D057134:Antibodies, Neutralizing; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000080908:Broadly Neutralizing Antibodies; C030325:anthrax toxin