Influence of portal blood on the development of systemic inflammation associated with experimental acute pancreatitis.
BACKGROUND
The liver is a source of systemic proinflammatory mediators in acute pancreatitis. We have investigated the effects of blood from the pancreas and intestine in liver activation and lung inflammation during early stages of experimental acute pancreatitis in a rat model.


METHODS
A portosystemic shunt and a mesosystemic shunt were created to prevent the passage of blood coming from the pancreas and the intestine, respectively, to the liver. Pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. After 3 hours, the inflammatory process in the lung and intestine, plasma levels of tumor necrosis factor (TNF)-alpha and their soluble receptor, and mRNA expression of inflammatory mediators in the lung were evaluated.


RESULTS
Portocaval shunting of blood prevented the inflammatory process in the lung, an increase in plasma TNF-alpha concentration, and the expression of TNF-alpha, interleukin (IL)-1beta, and heat-shock protein (HSP)-72 in the lung, but had no effect on plasma levels of soluble TNF-alpha receptor or on expression of inducible nitric oxide synthase (iNOS) and macrophage inflammatory protein (MIP)-2 in the lung. In contrast, mesocaval shunting of blood did not modify any of the parameters evaluated.


CONCLUSIONS
Pancreatic blood, but not intestinal blood, plays a key role in liver activation during experimental acute pancreatitis.
Hoyos
Sergio
S
Department of Surgery, Hospital Clinic, University of Barcelona, c/Rosselló 161, 7o 08036 Barcelona, Spain.
Granell
Susana
S
Heredia
Nicolas
N
Bulbena
Oriol
O
Closa
Daniel
D
Fernández-Cruz
Laureano
L
D000208:Acute Disease; D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D007422:Intestines / Q000098:blood supply; D008099:Liver / Q000098:blood supply; D008168:Lung / Q000276:immunology; D008297:Male; D010179:Pancreas / Q000098:blood supply; D010195:Pancreatitis / Q000097:blood* / Q000209:etiology* / Q000235:genetics / Q000276:immunology; D011168:Portal System; D011170:Portasystemic Shunt, Surgical; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018836:Inflammation Mediators; D012333:RNA, Messenger; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha