Activity of olive oil phenols on lymphomonocyte cytosolic calcium.
Phenols, present in the Mediterranean diet, have antioxidant properties and are free radical scavengers; however, the molecular mechanisms of their beneficial effects are not yet fully understood. The level of cytosolic calcium ([Ca2+]i) is an important signal also in nonexcitable cells, including immune cells, and regulates fundamental processes. In this paper, we determine [Ca2+]i in human lymphomonocytes incubated with two olive oil phenols: 3,4-(dihydroxyphenyl)ethanol and p-(hydroxyphenyl)ethanol. Both tested phenols increase [Ca2+]i in a dose-dependent way. This effect is antagonized by nifedipine and is noticeable both in the presence and in the absence of calcium in the extracellular medium.
Palmerini
Carlo A
CA
Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Università di Perugia, Via del Giochetto, 06127 Perugia, Italy. arienti@unipg.it
Carlini
Enrico
E
Saccardi
Carla
C
Servili
Maurizio
M
Montedoro
Ginafrancesco
G
Arienti
Giuseppe
G
D002118:Calcium / Q000008:administration & dosage / Q000032:analysis*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects; D003600:Cytosol / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure*; D009000:Monocytes / Q000187:drug effects / Q000648:ultrastructure*; D009543:Nifedipine / Q000494:pharmacology; D000069463:Olive Oil; D010636:Phenols / Q000494:pharmacology*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; C005975:3,4-dihydroxyphenylethanol; C011867:4-hydroxyphenylethanol; D009543:Nifedipine; D010626:Phenylethyl Alcohol; D002118:Calcium