Behaviour of dioxin in pig adipocytes.
Due to their lipophilic properties, dioxins can be integrated into the lipidic vacuole of adipocytes (fat cells). The aim of this study was to determine the kinetics of incorporation and release of 3H-labelled palmitic acid and 14C-labelled 2,3,7,8-TCDD in isolated adipocytes from pigs. The incorporation of 2,3,7,8-TCDD and palmitic acid was found to be concomitant under conditions of lipogenesis, under the effect of increasing quantities of insulin and in the presence of glucose. Release of these two compounds was found to be dependant on a lipolytic agent (epinephrine). These results suggest the risk of a strong increase of 2,3,7,8-TCDD, induced by lipolysis, in the blood of animals or humans previously exposed to this dioxin.
Irigaray
P
P
Laboratoire de Sciences Animales, INRA-INPL-UHP, Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires (ENSAIA), 2, 54 500 Vandoeuvre-Lès-Nancy, France. philippe.irigaray@ensaia.inpl-nancy.fr
Mejean
L
L
Laurent
F
F
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D004785:Environmental Pollutants / Q000378:metabolism / Q000493:pharmacokinetics*; D004837:Epinephrine / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D050356:Lipid Metabolism; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology; D019308:Palmitic Acid / Q000378:metabolism / Q000493:pharmacokinetics; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism / Q000493:pharmacokinetics*; D013552:Swine; D014316:Tritium
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D004785:Environmental Pollutants; D007328:Insulin; D000072317:Polychlorinated Dibenzodioxins; D014316:Tritium; D019308:Palmitic Acid; D004837:Epinephrine