Expression profiling of sciatic nerve in a Charcot-Marie-Tooth disease type 1a mouse model.
Expression profiling was performed on sciatic nerve of normal mice and of transgenic mice overexpressing the peripheral myelin protein 22 kDa (PMP22). These mice represent a model for the hereditary peripheral neuropathy Charcot-Marie Tooth type 1A. Comparison of the profiles reveals that the proteasomal degradation pathway and various signaling mechanisms are up-regulated in the diseased nerve. The down-regulated processes represent cell shape and adhesion as well as cellular activity and metabolism. In addition, we found that the most significantly up-regulated differences could not be mapped on known transcripts and thus might represent not identified transcripts. Our data will be helpful to direct future research aimed at deciphering the molecular pathogenesis of the most prevalent hereditary peripheral neuropathy.
ten Asbroek
Anneloor L M A
AL
Neurogenetics Laboratory, Academic Medical Center, Amsterdam, The Netherlands. a.l.tenasbroek@amc.uva.nl
Verhamme
Camiel
C
van Groenigen
Marjon
M
Wolterman
Ruud
R
de Kok-Nazaruk
Maryla M
MM
Baas
Frank
F
D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002607:Charcot-Marie-Tooth Disease / Q000378:metabolism*; D020934:Ciliary Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D017630:Connexins / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism; D018991:Myelin Proteolipid Protein / Q000235:genetics / Q000378:metabolism; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism; D012584:Sciatic Nerve / Q000378:metabolism* / Q000473:pathology; D000097002:Gap Junction beta-1 Protein
D020934:Ciliary Neurotrophic Factor; D017630:Connexins; D047908:Intracellular Signaling Peptides and Proteins; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D000097002:Gap Junction beta-1 Protein; C047516:Plp1 protein, mouse; C489545:Pmp22 protein, mouse