title: Effect of bone marrow-derived monocytes transfected with RNA of mouse colon carcinoma on specific antitumor immunity.
abstract: OBJECTIVE
  To investigate the effect of bone marrow-derived monocytes transfected with RNA of CT-26 (a cell line of mouse colon carcinoma) on antitumor immunity.
  
  
  METHODS
  Mouse bone marrow-derived monocytes were incubated with mouse granulocyte macrophage colony stimulating factor (mGM-CSF) in vitro, and the purity of monocytes was detected by flow cytometry. Total RNA of CT-26 was obtained by TRIzol's process, and monocytes were transfected by TransMessenger in vitro. The activity of cytotoxic T lymphocytes (CTL) in vivo was estimated by the modified lactate dehydrogenase (LDH) release assay. Changes of tumor size in mice and animal's survival time were observed in different groups.
  
  
  RESULTS
  Monocytes from mouse bone marrow were successfully incubated, and the positive rate of CD11b was over 95%. Vaccination of the monocytes transfected with total RNA induced a high level of specific CTL activity in vivo, and made mice resistant to the subsequent challenge of parental tumor cells. In vivo effects induced by monocytes transfected with total RNA were stronger than those induced by monocytes pulsed with tumor cell lysates.
  
  
  CONCLUSIONS
  Antigen presenting cells transfected with total RNA of CT-26 can present endogenous? tumor antigens, activate CTL, and effectively induce specific antitumor immunity.
authors.affiliation: Department of Oncology, Nanjing General Hospital of PLA, Nanjing 210002, Jiangsu Province, China. zxy_6906@tom.com
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Chu
authors.forename: Long-Bang
authors.initials: LB
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Zang
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Huai-Cheng
authors.initials: HC
authors.lastname: Geng
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000276:immunology* / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods; D007167:Immunotherapy / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D012334:RNA, Neoplasm / Q000276:immunology*; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D012334:RNA, Neoplasm