Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana.
Two solanesyl diphosphate synthases, designated SPS1 and SPS2, which are responsible for the synthesis of the isoprenoid side chain of either plastoquinone or ubiquinone in Arabidopsis thaliana, were identified. Heterologous expression of either SPS1 or SPS2 allowed the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli. SPS1-GFP was found to localize in the ER while SPS2-GFP localized in the plastid of tobacco BY-2 cells. These two different subcellular localizations are thought to be the reflection of their roles in solanesyl diphosphate synthesis in two different parts: presumably SPS1 and SPS2 for the side chains of ubiquinone and plastoquinone, respectively.
Jun
Luo
L
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, 690-8504 Japan.
Saiki
Ryoichi
R
Tatsumi
Kei
K
Nakagawa
Tsuyoshi
T
Kawamukai
Makoto
M
D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004925:Escherichia / Q000378:metabolism; D008969:Molecular Sequence Data; D018087:Plastids / Q000378:metabolism; D010971:Plastoquinone / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013729:Terpenes / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism; D014451:Ubiquinone / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D013729:Terpenes; D014451:Ubiquinone; D019883:Alkyl and Aryl Transferases; C039821:decaprenyl pyrophosphate synthetase; C022644:trans-octaprenyltranstransferase; C500830:SPS1 protein, Arabidopsis; C500831:SPS2 protein, Arabidopsis; C030571:ubiquinone 9; D010971:Plastoquinone