title: [Effect of tanshinone microemulsion on reversing MDR in human tumor cells].
abstract: OBJECTIVE
  To study the effect of tanshinone microemulsion (Tan-M) on the cytotoxicity to human leukemia-cell-line (K562/ADM) and the reversion of MDR in vitro.
  
  
  METHODS
  Microemulsion being supposed as the control group, MT method is adopted to test cytotoxicity and the reverse of MDR.
  
  
  RESULTS
  Obvious cytotoxicity to K562/ADM was observed for tan-M. Cell non-toxic dosage (growth quotiety > 95%) of Tan-M is 0.2 microg x mL(-1). Low toxic dosage (growth quotiety 85-90%) was 0.7 microg x mL(-1). Cell non-toxic dosage of was 0.7 microg x mL(-1) and low toxic dosage was 1.2 microg x mL(-1). Cell non-toxic dosage of Tan-M (0.2 microg x mL(-1)) significantly lowered the IC50 of K562/ADM by ADM (P < 0.01), and reversed MDR was 3.88 times. Low toxic dosage of Tan-M reversed MDR was 3.97 times. E-M (0.2 microg x mL(-1)) reversed MDR was 2.62 times.
  
  
  CONCLUSIONS
  The result indicates that tanshinone microemulsion possesses cell-toxic effects on human leukemia cell-line and may reverse MDR of tumor cells.
authors.affiliation: Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China. FQ731@sina.com.cn
authors.forename: Qing
authors.initials: Q
authors.lastname: Fan
authors.forename: Guang-jun
authors.initials: GJ
authors.lastname: Fan
authors.forename: Pei-man
authors.initials: PM
authors.lastname: Yang
authors.forename: Jin-yao
authors.initials: JY
authors.lastname: Zhao
mesh_terms: D045784:Abietanes; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D004655:Emulsions; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D010616:Phenanthrenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D045784:Abietanes; D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D010616:Phenanthrenes; C021751:tanshinone; D004317:Doxorubicin