Suppressed TGF-beta1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment.
OBJECTIVE
Flashlamp pulsed-dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor-beta1 (TGF-beta1)-induction and up-regulation of matrix metalloproteinase (MMP) expression in keloid regression.


METHODS
Keloid tissues obtained from 10 patients with intra-lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10-18 J/cm2 (mean 14.0 J/cm2)]. Immunohistochemical (IHC) staining of TGF-beta1 and MMP-1 and MMP-13 expressions in keloid tissue was performed. Western blot analysis of MMP-1 and MMP-13 expressions in extracellular matrix was evaluated.


RESULTS
IHC staining indicated that expression of TGF-beta1 was significantly reduced in keloid tissues after PDL irradiation. MMP-13 but not MMP-1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP-13 but not MMP-1 significant increased in keloid tissues after PDL treatment.


CONCLUSIONS
Regression of keloids regressed after PDL treatments are associated with down-regulation of TGF-beta1 expression and up-regulation of MMP-13 activity.
Kuo
Yur-Ren
YR
Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
Wu
Wen-Shan
WS
Jeng
Seng-Feng
SF
Wang
Feng-Sheng
FS
Huang
Hui-Chen
HC
Lin
Cha-Zon
CZ
Yang
Kuender D
KD
D000293:Adolescent; D000328:Adult; D002648:Child; D017364:Collagenases / Q000378:metabolism* / Q000528:radiation effects; D015536:Down-Regulation / Q000528:radiation effects; D005260:Female; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D053685:Laser Therapy; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism* / Q000528:radiation effects; D053509:Matrix Metalloproteinase 13; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000378:metabolism* / Q000528:radiation effects; D053773:Transforming Growth Factor beta1; D015854:Up-Regulation / Q000528:radiation effects
C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D020781:Matrix Metalloproteinase 1