title: Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea.
abstract: Three moderately halophilic, spore-forming strains, designated BH030062T, BH030049 and BH030080, were isolated from a solar saltern in Korea. Phylogenetic analyses and comparative 16S rRNA gene sequence studies revealed that the isolates represent a novel distinct monophyletic lineage within the phyletic group classically defined as the genus Bacillus and are most closely related to members of the genera Gracilibacillus (93.7-95.1 % similarity), Virgibacillus (93.5-94.8 %), Halobacillus (94.8-95.9 %), Filobacillus (94.4-94.8 %) and Lentibacillus (93.3-93.7 %). Strain BH030062T was strictly aerobic, rod-shaped, Gram-positive and motile by means of peritrichous flagella. It grew in the presence of 1-15 % (w/v) NaCl and at temperatures of 15-45 degrees C. The cell wall peptidoglycan contained A1gamma-meso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids were iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(16 : 0). DNA G+C content was about 41 mol% and the major isoprenoid quinone was MK-7. On the basis of their physiological and molecular properties, the isolates represent a new genus, Pontibacillus gen. nov., and novel species, Pontibacillus chungwhensis sp. nov. The type strain is BH030062T (=KCTC 3890T=DSM 16287T).
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeundong, Yusong, Daejon 305-333, Republic of Korea.
authors.forename: Jee-Min
authors.initials: JM
authors.lastname: Lim
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeundong, Yusong, Daejon 305-333, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeundong, Yusong, Daejon 305-333, Republic of Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Song
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeundong, Yusong, Daejon 305-333, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride