FPF1 transgene leads to altered flowering time and root development in rice.
AtFPF1 (FLOWERING PROMOTING FACTOR 1) is a gene that promotes flowering in Arabidopsis. An expression vector containing AtFPF1 driven by a Ubi-1 promoter was constructed. The gene was introduced into rice callus by Agrobacterium-mediated transformation and fertile plants were obtained. The presence of AtFPF1 in rice plants was confirmed by PCR, Southern and Northern blot analyses, as well as by beta-glucuronidase assay. The results showed that, as in Arabidopsis, AtFPF1 reduced flowering time in rice. Furthermore, introduction of AtFPF1 enhanced adventitious root formation but inhibited root growth in rice during the seedling stage. The results suggest that AtFPF1 promotes flowering time in both dicots and monocots, and plays a role in the initiation of adventitious roots in rice.
Xu
Ming-Li
ML
Research Center for Molecular & Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Chinese Academy of Sciences, Beijing, 100093, China.
Jiang
Jia-Fu
JF
Ge
Lei
L
Xu
Yun-Yuan
YY
Chen
Hui
H
Zhao
Yuan
Y
Bi
Yu-Rong
YR
Wen
Jiang-Qi
JQ
Chong
Kang
K
D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D018745:Genome, Plant; D005966:Glucuronidase / Q000378:metabolism; D006131:Growth Inhibitors / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000254:growth & development*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013997:Time Factors; D014170:Transformation, Genetic / Q000235:genetics
D029681:Arabidopsis Proteins; C108185:FPF1 protein, Arabidopsis; D006131:Growth Inhibitors; D005966:Glucuronidase