title: Column selectivity in reversed-phase liquid chromatography. VIII. Phenylalkyl and fluoro-substituted columns.
abstract: As reported previously, five solute-column interactions (hydrophobicity, steric resistance, hydrogen-bond acidity and basicity, ionic interaction) quantitatively describe column selectivity for 163 alkyl-silica, polar-group and cyano columns. In the present study, solute retention and column selectivity for 11 phenyl and 5 fluoro-substituted columns were compared with alkyl-silica columns of similar ligand length. It is concluded that two additional solute-column interactions may be significant in affecting retention and selectivity for the latter columns: (a) dispersion interactions of varying strength as a result of significant differences in bonded-phase polarizability or refractive index and (b) pi-pi interactions in the case of phenyl columns and aromatic solutes. These 16 phenyl and fluoro columns were also characterized in terms of hydrophobicity, steric resistance, hydrogen-bond acidity and basicity, and ionic interaction.
authors.affiliation: Department of Chemistry, University Wisconsin - River Falls, River Falls, WI 54022, USA.
authors.forename: D H
authors.initials: DH
authors.lastname: Marchand
authors.forename: K
authors.initials: K
authors.lastname: Croes
authors.forename: J W
authors.initials: JW
authors.lastname: Dolan
authors.forename: L R
authors.initials: LR
authors.lastname: Snyder
authors.forename: R A
authors.initials: RA
authors.lastname: Henry
authors.forename: K M R
authors.initials: KM
authors.lastname: Kallury
authors.forename: S
authors.initials: S
authors.lastname: Waite
authors.forename: P W
authors.initials: PW
authors.lastname: Carr
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation*; D006860:Hydrogen Bonding; D012031:Refractometry; D012680:Sensitivity and Specificity