title: Sequence of a novel HLA-A*0301 intronic variant (A*03010103).
abstract: We report here the full-length sequence of a novel HLA-A*0301 allele, A*03010103, which differs from A*03010101 by a single nucleotide substitution (G>T) at position 492 within intron 2. The variant was originally identified by Reference Strand-mediated Conformational Analysis (RSCA) and was confirmed by cloning and sequencing. The difference in RSCA mobility between A*03010101 and A*03010103 demonstrates the sensitivity of RSCA to detect single nucleotide polymorphisms.
authors.affiliation: The Anthony Nolan Research Institute, The Royal Free Hospital, Hampstead, London, UK.
authors.forename: N P
authors.initials: NP
authors.lastname: Mayor
authors.forename: S T
authors.initials: ST
authors.lastname: Cox
authors.forename: A J
authors.initials: AJ
authors.lastname: McWhinnie
authors.forename: J R
authors.initials: JR
authors.lastname: Argüello
authors.forename: B E
authors.initials: BE
authors.lastname: Shaw
authors.forename: A-M
authors.initials: AM
authors.lastname: Little
authors.forename: J A
authors.initials: JA
authors.lastname: Madrigal
authors.forename: S G E
authors.initials: SG
authors.lastname: Marsh
mesh_terms: D001483:Base Sequence; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D015791:HLA-A3 Antigen; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data
chemical_list: D015234:HLA-A Antigens; C442141:HLA-A*03:01 antigen; D015791:HLA-A3 Antigen