title: Effects of oxidized LDL on in vitro proliferation and spontaneous motility of human coronary artery endothelial cells.
abstract: OBJECTIVE
  To investigate the effects of low concentrations of oxidized LDL (oxLDL) on the proliferation and spontaneous motility of human coronary artery endothelial cells (HCAEC) in culture.
  
  
  METHODS
  Cultures of HCAEC were treated with low concentrations of native LDL (nLDL) isolated from human plasma and with LDL minimally oxidized through different chemical methods; the effects were compared.
  
  
  RESULTS
  Native LDL had no deleterious effects on in vitro proliferation and motility of HCAEC; however, at its highest concentration and for a longer exposure, nLDL inhibited cell proliferation. The LDL chemically oxidized by spermine nonoate (SNO) and 3-morpholinylsydnonimine (SYN-1) had significant inhibiting effects on in vitro proliferation and motility of HCAEC, which were proportional to the greatest concentrations and degrees of oxidation of LDL.
  
  
  CONCLUSIONS
  OxLDL has a cytotoxic effect, inhibiting the proliferation and spontaneous motility of HCAEC in culture. This effect is proportional to the concentration and degree of oxidation of LDL; native LDL is relatively innocuous.
authors.affiliation: Instituto do Coração, Faculdade de Medicina, USP, São Paulo, SP, Brazil. htxavier@cardiol.br
authors.forename: Hermes Toros
authors.initials: HT
authors.lastname: Xavier
authors.forename: Dulcinéia Saes Parra
authors.initials: DS
authors.lastname: Abdalla
authors.forename: Tania Leme da Rocha
authors.initials: TL
authors.lastname: Martinez
authors.forename: José Antonio Franchini
authors.initials: JA
authors.lastname: Ramires
authors.forename: Antonio Ricardo de Toledo
authors.initials: AR
authors.lastname: Gagliardi
mesh_terms: D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D003324:Coronary Artery Disease / Q000378:metabolism / Q000503:physiopathology; D003331:Coronary Vessels / Q000166:cytology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein