A new trichrome staining method: its practice and application in normal tissues.
A new trichrome staining method is presented. The staining solution contains: chromotrope RH, lissamine green SF, phosphomolibdic acid and acetic acid in aquous solution. The steps of the technique are described, insisting on the staining solution and dehydration. The authors revealed the staining properties of the method in different tissue and organs. The importance of the method for the histological study and its possible applications in pathology is discussed.
Raica
M
M
Department of Histology and Cytology, Timişoara University of Medicine and Pharmacy.
Mederle
O
O
Raţ
G
G
D019342:Acetic Acid; D006801:Humans; D008086:Lissamine Green Dyes; D008982:Molybdenum; D009282:Naphthalenesulfonates; D016612:Paraffin Embedding; D010756:Phosphoric Acids; D013194:Staining and Labeling; D016707:Tissue Fixation
D008086:Lissamine Green Dyes; D009282:Naphthalenesulfonates; D010756:Phosphoric Acids; C070620:chromotrope 2R; D008982:Molybdenum; D019342:Acetic Acid; C003125:phosphomolybdic acid