Strain differentiation of Trichophyton rubrum by random amplification of polymorphic DNA (RAPD).
Trichophyton rubrum is an important cause of dermatomycoses. Molecular strain typing methods have recently been developed to address questions about epidemiology and source of relapse following treatment. This report describes the application of RAPD for molecular strain differentiation of this fungus utilizing the primers 1- (5'-d[GGTGCGGGAA]-3') and 6- (5'-d[CCCGTCAGCA]-3'). A total of five RAPD patterns were observed among 10 strains of T. rubrum, with each of the primers used. We conclude that RAPD analysis using primers 1 and 6 can be used in epidemiological studies.
Baeza
Lilian Cristiane
LC
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, 14801-902 Araraquara, SP, Brazil.
Giannini
Maria José Soares Mendes
MJ
D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques; D019105:Random Amplified Polymorphic DNA Technique; D014249:Trichophyton / Q000145:classification* / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal