title: Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells.
abstract: OBJECTIVE
  To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co-stimulatory molecule expression and interleukin-12 (IL-12) production from THP-1 cells.
  
  
  METHODS
  THP-1 cells were differentiated into macrophage-like cells by Me2SO, and then cultured with IFN-gamma (500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on a FACScan flow cytometer. IL-12p40, IL-12p70 were assayed by ELISA.
  
  
  RESULTS
  Triptolide suppressed CD80 and CD86 expressions on IFN-gamma (500 kU/L) and LPS (1 mg/L) activated THP-1 cells at nontoxic dosages of 2.5-0.625 microg/L. Furthermore, the production of IL-12p40 and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide.
  
  
  CONCLUSIONS
  Triptolide impairs the antigen-presenting function by inhibiting CD80 and CD86 expressions and decreased IL-12p40 and IL-12p70 (bioactive form) productions from the activated THP-1 cells.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Wu
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Feng
authors.forename: Yi-Fu
authors.initials: YF
authors.lastname: Wang
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zuo
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism*; D051940:B7-2 Antigen; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004852:Epoxy Compounds; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D018664:Interleukin-12 / Q000096:biosynthesis*; D053762:Interleukin-12 Subunit p40; D007951:Leukemia, Myeloid / Q000473:pathology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D021122:Protein Subunits / Q000096:biosynthesis; D029757:Tripterygium / Q000737:chemistry
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D004224:Diterpenes; D004852:Epoxy Compounds; D007166:Immunosuppressive Agents; D053762:Interleukin-12 Subunit p40; D008562:Membrane Glycoproteins; D010616:Phenanthrenes; D021122:Protein Subunits; D018664:Interleukin-12; C001899:triptolide