Protective effect of a low K+ cardioplegic solution on myocardial Na,K-ATPase activity.
Long duration ischemia in hypothermic conditions followed by reperfusion alters membrane transport function and in particular Na,K-ATPase. We compared the protective effect of two well-described cardioplegic solutions on cardiac Na,K-ATPase activity during reperfusion after hypothermic ischemia. Isolated perfused rat hearts (n = 10) were arrested with CRMBM or UW cardioplegic solutions and submitted to 12 hr of ischemia at 4 degrees C in the same solution followed by 60 min of reperfusion. Functional recovery and Na,K-ATPase activity were measured at the end of reperfusion and compared with control hearts and hearts submitted to severe ischemia (30 min at 37 degrees C) followed by reflow. Na,K-ATPase activity was not altered after 12 hr of ischemia and 1 hr reflow when the CRMBM solution was used for preservation (55 +/- 2 micromolPi/mg prot/hr) compared to control (53 +/- 2 micromol Pi/mg prot/hr) while it was significantly altered with UW solution (44 +/- 2 micromol Pi/mg prot/hr, p < 0.05 vs control and CRMBM). Better preservation of Na,K-ATPase activity with the CRMBM solution was associated with higher functional recovery compared to UW as represented by the recovery of RPP, 52 +/- 12% vs 8 +/- 5%, p < 0.05 and coronary flow (70 +/- 2% vs 50 +/- 8%, p < 0.05). The enhanced protection provided by CRMBM compared to UW may be related to its lower K+ content.
Bernard
M
M
Centre de Resonance Magnétique Biologique et Médicale, CNRS UMR 6612, Faculté de Médecine de Marseille, 27 Bd Jean Moulin, 13005 Marseille, France. monique.bernard@medecine.univ-mrs.fr
Robert
K
K
Caus
T
T
Desrois
M
M
Paganelli
F
F
Cozzone
P J
PJ
Maixent
J M
JM
D000241:Adenosine / Q000737:chemistry; D000493:Allopurinol / Q000737:chemistry; D000818:Animals; D002314:Cardioplegic Solutions / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D019188:Organ Preservation Solutions / Q000737:chemistry; D011188:Potassium / Q000032:analysis*; D020011:Protective Agents / Q000737:chemistry*; D011887:Raffinose / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
C099594:CRMBM solution; D002314:Cardioplegic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D020011:Protective Agents; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D011188:Potassium