Hybridization dependent cleavage of internally modified disulfide-peptide nucleic acids.
Selectivity of the cleavage of single stranded over hybridized forms of internally modified disulfide-peptide nucleic acids (PNA) has been optimized using a series of phosphines and thiols, which have different sizes and charges. For the most selective cleaver found (tris-(carboxyethyl)-phosphine), reactivity of single stranded PNA is 33 times higher than that of the PNA-DNA duplex. Selectivity of single stranded disulfide-PNA cleavage has been explained in terms of electrostatic interaction between the substrate and the cleaver.
Boll
Iris
I
Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 270, D-69120 Heidelberg, Germany.
Krämer
Roland
R
Mokhir
Andriy
A
D004247:DNA / Q000737:chemistry*; D004220:Disulfides; D006868:Hydrolysis; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010720:Phosphines; D013438:Sulfhydryl Compounds
D004220:Disulfides; D020135:Peptide Nucleic Acids; D010720:Phosphines; D013438:Sulfhydryl Compounds; D004247:DNA