Role of phosphatidylinositol-3-kinase and protein kinase C zeta in the adenylate cyclase signal mechanism of action of relaxin in muscle tissues of rats and mollusks.
We showed that phosphatidylinositol-3-kinase and protein kinase C zeta are involved in the adenylate cyclase signal mechanism of relaxin action. A selective inhibitor of phosphatidylinositol-3-kinase wortmannin blocked the stimulatory effect of relaxin on adenylate cyclase in rat skeletal muscles and Anodonta cygnea smooth muscles. Antibodies against protein kinase C zeta abolished the relaxin-induced stimulation of adenylate cyclase in rat muscles, but not in mollusk muscles. Our results indicate that phosphatidylinositol-3-kinase and protein kinase C zeta play a role in the adenylate cyclase signal mechanism of relaxin action.
Shpakov
A O
AO
Laboratory of Molecular Endocrinology, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg. shpakov@hormone.ief.spb.su
Kuznetsova
L A
LA
Plesneva
S A
SA
Pertseva
M N
MN
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008974:Mollusca / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D012065:Relaxin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D000077191:Wortmannin; D000099221:Protein Kinase C zeta
D000262:Adenylyl Cyclases; D000730:Androstadienes; D004791:Enzyme Inhibitors; D007328:Insulin; D019869:Phosphatidylinositol 3-Kinases; D011493:Protein Kinase C; D012065:Relaxin; D000077191:Wortmannin; D000099221:Protein Kinase C zeta