LDOC1, a novel MZF-1-interacting protein, induces apoptosis.
LDOC1 was isolated as a gene encoding a leucine-zipper protein whose expression was decreased in pancreatic and gastric cancer cell lines in 1999. Here, we found that overexpression of LDOC1 caused externalization of the cell membrane phosphatidylserine, which was characteristic for early-phase apoptotic events, and reduced cell viability in some human cell lines. The apoptotic process was triggered by a loss of the mitochondrial membrane potential, leading to both caspase-3-dependent and -independent pathways. Furthermore, a transcription factor, MZF-1, was revealed to interact with LDOC1 and enhance the activity of LDOC1 for inducing apoptosis.
Inoue
Mikiya
M
Department of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, Nihon University Graduate School of Medical Sciences, 30-1 Oyaguchi, Kami-machi, Itabashi-ku, Toyko 173-8610, Japan.
Takahashi
Kyoko
K
Niide
Osamu
O
Shibata
Masahiko
M
Fukuzawa
Masahiro
M
Ra
Chisei
C
D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000502:physiology*; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; C120625:LDOC1 protein, human; C085969:MZF1 protein, human; D009687:Nuclear Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D020169:Caspases