Single-molecule fluorescence spectroscopy: new probes of protein function and dynamics.
Single-molecule fluorescence methods provide new tools for the study of biological systems. Single-pair fluorescence resonance energy transfer has provided detailed information about dynamics and structure of the Ca2+-signaling protein calmodulin. Single-molecule polarization modulation spectroscopy has probed the mechanism by which calmodulin activates the plasma membrane Ca2+ pump.
Johnson
Carey K
CK
Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA. ckjohnson@ku.edu
Osborn
Kenneth D
KD
Allen
Michael W
MW
Slaughter
Brian D
BD
D000818:Animals; D020013:Calcium Signaling; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D013050:Spectrometry, Fluorescence
D002147:Calmodulin; D000252:Calcium-Transporting ATPases