Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters.
Two methanogenic strains, 8-2T and 4-1, with rod-shaped (0.4-0.5 x 3-5 microm), non-motile cells, sometimes observed in chains, were isolated from two anaerobic digesters in Beijing, China. The two strains used H2/CO2 and formate for growth and produced methane. The temperature range for growth was 25-50 degrees C, with fastest growth at 37 degrees C. The pH ranges for growth and methane production were 6.5-8.0 for strain 8-2T and 6.8-8.6 for strain 4-1, with the fastest growth at pH 7.2 for strain 8-2T and pH 7.5-7.7 for strain 4-1. The G+C content of genomic DNA for strain 8-2T was 38.9 mol%. The similarity levels of the 16S rRNA sequence of strain 8-2T with other species of the genus Methanobacterium ranged from 93.8 to 96.0 %. Based on the phylogenetic analysis and phenotypic characteristics, the novel species Methanobacterium beijingense sp. nov. is proposed, with the type strain 8-2T (=DSM 15999T=CGMCC 1.5011T).
Ma
Kai
K
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Liu
Xiaoli
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Dong
Xiuzhu
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
D000693:Anaerobiosis; D019149:Bioreactors; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008697:Methane / Q000378:metabolism*; D017014:Methanobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008697:Methane