title: Frat is dispensable for canonical Wnt signaling in mammals.
abstract: Wnt-signal transduction through beta-catenin is thought to require the inhibition of GSK3 by Frat/GBP. To investigate the role of Frat in mammalian development, we have generated mice with targeted mutations in all three murine Frat homologs. We show that Frat is normally expressed at sites of active Wnt signaling. Surprisingly, Frat-deficient mice do not display gross abnormalities. Moreover, canonical Wnt signaling in primary cells is unaffected by the loss of Frat. These studies show that Frat is not an essential component of the canonical Wnt pathway in higher organisms, despite the strict requirement of Frat/GBP for maternal Wnt signaling in Xenopus.
authors.affiliation: Division of Molecular Genetics, Centre of Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX, Amsterdam, The Netherlands.
authors.forename: Renée
authors.initials: R
authors.lastname: van Amerongen
authors.forename: Martijn
authors.initials: M
authors.lastname: Nawijn
authors.forename: Jonathan
authors.initials: J
authors.lastname: Franca-Koh
authors.forename: John
authors.initials: J
authors.lastname: Zevenhoven
authors.forename: Hanneke
authors.initials: H
authors.lastname: van der Gulden
authors.forename: Jos
authors.initials: J
authors.lastname: Jonkers
authors.forename: Anton
authors.initials: A
authors.lastname: Berns
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D011518:Proto-Oncogene Proteins; D015398:Signal Transduction / Q000502:physiology*; D051153:Wnt Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C491818:Frat1 protein, mouse; C488487:Frat2 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; C491814:Peg12 protein, mouse; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins