title: High-resolution sequence-based DPA1 typing identified two novel DPA1 alleles, DPA1*010303 and DPA1*0303, from a Kenyan population.
abstract: We report here two novel DPA1 alleles, DPA1*010303 and DPA1*0303, identified from a Kenyan population during sequence-based HLA-DPA1 typing. Molecular cloning and sequencing of multiple clones confirmed that one of the new DPA1 alleles is identical to DPA1*010301 at exon 2, except for a single nucleotide substitution (ACG ACC) at codon 15. The new allele has been named by the WHO Nomenclature Committee as DPA1*010303. The second novel DPA1 allele is identical to DPA1*0301, except for a single nucleotide difference (GAA GAC) at codon 28 that changed the amino acid from Glu to Asp. The new allele has been named by the WHO Nomenclature Committee as DPA1*0303. Identification of the two novel DPA1 alleles reflects the genetic diversity of this East African population.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: J
authors.initials: J
authors.lastname: Bamforth
authors.forename: K
authors.initials: K
authors.lastname: Gill
authors.forename: C
authors.initials: C
authors.lastname: Cohen
authors.forename: R C
authors.initials: RC
authors.lastname: Brunham
authors.forename: F A
authors.initials: FA
authors.lastname: Plummer
mesh_terms: D001483:Base Sequence; D006682:HLA-DP Antigens / Q000235:genetics* / Q000276:immunology; D059867:HLA-DP alpha-Chains; D006801:Humans; D007630:Kenya; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D006682:HLA-DP Antigens; D059867:HLA-DP alpha-Chains; C071172:HLA-DPA1 antigen