title: Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b.
abstract: A novel gene for quinolone resistance was cloned from a transferable plasmid carried by a clinical isolate of Shigella flexneri 2b that was resistant to fluoroquinolones. The plasmid conferred low-level resistance to quinolones on Escherichia coli HB101. The protein encoded by the gene showed 59% amino acid identity with Qnr.
authors.affiliation: Department of Microbiology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsujimachi, Kita-ku, Nagoya, Aichi 462-8576, Japan. mami_hata@pref.aichi.lg.jp
authors.forename: Mami
authors.initials: M
authors.lastname: Hata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Suzuki
authors.forename: Masakado
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Masao
authors.initials: M
authors.lastname: Takahashi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Sato
authors.forename: Shiro
authors.initials: S
authors.lastname: Ibe
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakae
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D015363:Quinolones / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012763:Shigella flexneri / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones