Luteolin induced DNA damage leading to human lung squamous carcinoma CH27 cell apoptosis.
Luteolin is an active compound from the Lonicera japonica (Caprifoliaceae). Luteolin (50 microM)-induced human lung carcinoma CH27 cell apoptosis is a typical apoptosis that was accompanied by a significant DNA condensation and apoptotic body formation. Luteolin-induced apoptosis is related to its ability to change the expression of apoptotic markers, such as caspase-3 (caspase-dependent) and apoptosis-inducing factor (caspase-independent) protein expression. The alkaline microgel electrophoresis technique (comet assay), which is the most sensitive, was used for estimation of the luteolin-induced DNA single strand breaks in this study. DNA-damaging effects of luteolin on DNA single strand breaks have been demonstrated in our study. In this study, luteolin induced S-phase cell cycle arrest and increased the mRNA of DNA repair enzymes such as human MutT homologue, 8-oxoguanine-glycosylase and apurinic endonuclease. Our data suggested that luteolin induces CH27 cell apoptosis by caspase-dependent and -independent pathway and the effect of luteolin on apoptosis of CH27 cells is associated with DNA damage and the expression of DNA repair enzymes.
Leung
Henry Wing-Cheung
HW
Department of Radiation Oncology, Chi Mei Medical Center, Tainan, Taiwan.
Wu
Chun-Hsiung
CH
Lin
Chia-Hsien
CH
Lee
Hong-Zin
HZ
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D051033:Apoptosis Inducing Factor; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D045647:DNA Glycosylases / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics; D004273:DNA, Neoplasm / Q000187:drug effects / Q000235:genetics; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005420:Flavoproteins / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D047311:Luteolin / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; D004273:DNA, Neoplasm; D005420:Flavoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D010744:Phosphoric Monoester Hydrolases; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; C078207:8-oxodGTPase; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D045643:DNA Repair Enzymes; D047311:Luteolin