DNA ploidy and S phase fraction of breast and ovarian tumor cells treated with a natural anthracycline analog (aloin).
DNA ploidy and S phase fraction analysis by flow cytometry on breast and ovarian tumor cells continuously exposed to aloin, a natural anthraquinone, at two concentrations (20-60 microg/ml) was done. Untreated breast and ovarian tumor cells (control) showed an aneuploid pattern, with a mean DNA index of 2.10+/-0.10 and S phase fraction of 28.46+/-1.5 and 17.40+/-0.75%, respectively. Treatment of breast and ovarian tumor cells with aloin showed a persistent aneuploid pattern and a significantly dose-dependent increase in the percentage of S phase fraction and in the proportion of cells cycling at a higher ploidy level (>G2M). The polyploidization indicates that aloin does not inhibit initiation of DNA synthesis and that cells replicated a full complement of DNA but had difficulty in M phase.
Esmat
Amr Y
AY
Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. amryesmat@hotmail.com
El-Gerzawy
Shadia M
SM
Rafaat
Amira
A
D000504:Aloe; D001943:Breast Neoplasms / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis; D004642:Emodin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D011003:Ploidies; D016196:S Phase / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D004247:DNA; D004642:Emodin; C006457:alloin