title: Analysis of recognition of transfer-messenger RNA by the ribosomal decoding center.
abstract: Bacterial ribosomes stalled on defective mRNAs are rescued by tmRNA that functions as both tRNA and mRNA. The first ribosomal elongation cycle on tmRNA where tmRNA functions as tRNA is highly unusual: occupation of the ribosomal A site by tmRNA occurs without codon:anticodon pairing. Our analysis shows that in this case the role of a codon:anticodon duplex should be accomplished by a single unpaired triplet. In order that tmRNA could participate in the ribosomal elongation cycle, a triplet preceding the mRNA portion of tmRNA (the -1triplet) should be in the A-form and this form should be recognized by the ribosomal decoding center. A rule is derived that determines what triplets cannot be used as the -1triplet. The rule was tested with the -1triplets of all known 414 tmRNA species. All 23 observed -1triplets follow the formulated rule. The rule is also supported by the available data on base substitutions within the -1triplet.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991 Moscow, Russian Federation.
authors.forename: Valery I
authors.initials: VI
authors.lastname: Lim
authors.forename: Maria B
authors.initials: MB
authors.lastname: Garber
mesh_terms: D001665:Binding Sites; D003062:Codon; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008962:Models, Theoretical; D012329:RNA, Bacterial / Q000502:physiology*; D012270:Ribosomes / Q000235:genetics*; D055672:Static Electricity
chemical_list: D003062:Codon; D012329:RNA, Bacterial; C096222:tmRNA