title: Identifying rodent hantavirus reservoirs, Brazil.
abstract: We describe the genetic analysis of samples from hantavirus pulmonary syndrome (HPS) patients from southern and southeastern states of Brazil and rodents captured at the presumed site of infection of these patients. A total of 65 samples that were antibody-positive for Sin Nombre or Laguna Negra virus by enzyme-linked immunosorbent assay were processed by nested reverse transcription-polymerase chain reaction (RT-PCR) by using several primer combinations in the M and S genome segments. PCR products were amplified and sequenced from samples from 11 HPS patient and 7 rodent samples. Phylogenetic analysis of nucleotide sequence differences showed the cocirculation of Araraquara and Juquitiba-like viruses, previously characterized from humans. Our genetic data indicate that Araraquara virus is associated with Bolomys lasiurus (hairy-tailed Bolo mouse) and the Juquitiba-like virus is associated with Oligoryzomys nigripes (black-footed pigmy rice rat).
authors.affiliation: Instituto Adolfo Lutz-São Paulo, São Paulo, Brazil. aksuzuki@ial.sp.gov.br
authors.forename: Akemi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Ivani
authors.initials: I
authors.lastname: Bisordi
authors.forename: Silvana
authors.initials: S
authors.lastname: Levis
authors.forename: Jorge
authors.initials: J
authors.lastname: Garcia
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Pereira
authors.forename: Renato P
authors.initials: RP
authors.lastname: Souza
authors.forename: Teresa K N
authors.initials: TK
authors.lastname: Sugahara
authors.forename: Noemi
authors.initials: N
authors.lastname: Pini
authors.forename: Delia
authors.initials: D
authors.lastname: Enria
authors.forename: Luiza T M
authors.initials: LT
authors.lastname: Souza
mesh_terms: D000328:Adult; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D004197:Disease Reservoirs; D005260:Female; D006476:Orthohantavirus / Q000235:genetics / Q000302:isolation & purification*; D018804:Hantavirus Pulmonary Syndrome / Q000453:epidemiology / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012377:Rodentia / Q000821:virology*
chemical_list: D012367:RNA, Viral