Transcriptional activation of cytochrome P450 1A1 with alpha-tocopherol.
Peroral administration of alpha-tocopherol in a daily dose of 150 mg/kg for 1, 4, 8, and 12 days leads to induction of cytochromes P450 1A in male rats. Activity of CYP1A1 and CYP1A2 increased most significantly one day after alpha-tocopherol administration (by 2.6 and 2.7 times, respectively). CYP1A1 was immunohistochemically detected in rat liver microsomes during this period. The content of CYP1A1 mRNA significantly increased in the liver. The amount of CYP1A2 mRNA and regulatory proteins for signal activation of CYP1A1 (AhR and Arnt) remained unchanged after treatment with alpha-tocopherol.
Sidorova
Yu A
YA
Laboratory for Biochemistry of Foreign Compounds, Institute of Molecular Biology and Biophysics, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Grishanova
A Yu
AY
Lyakhovich
V V
VV
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D019388:Cytochrome P-450 CYP1A2 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000276:immunology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D015533:Transcriptional Activation; D024502:alpha-Tocopherol / Q000494:pharmacology*
D012333:RNA, Messenger; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2; D024502:alpha-Tocopherol