Cytotoxicity of two novel formulations of calcium phosphate cements: a comparative in vitro study.
The purpose was to evaluate the cytotoxicity of two novel formulations (alpha and beta) of calcium phosphate cements. Positive control, represented by a commercial hydroxyapatite cement, and negative control were included for comparative purposes. A continuous lineage of fibroblastic cells was used, and the effect of the tested materials on both cell proliferation and viability was assessed by counting cell number on hemocytometer and by the trypan blue exclusion test, respectively. Study design attempted to simulate clinical use by allowing direct and indirect contact of cells and cements. Results were analyzed by the Kruskal-Wallis test and indicated that the beta formulation was extremely cytotoxic (P < 0.001), because this material induced the greatest reduction on cell proliferation and viability. The alpha formulation behaved similarly to the positive control regarding its effect on cell proliferation and viability. Thus, it is concluded that alpha formulation has promise for further evaluation of its behavior in vivo.
Rossa
Carlos
C
Department of Periodontics, School of Dentistry at Araraquara, State University of São Paulo, Araraquara, São Paulo, Brazil. crossa@umich.edu
Marcantonio
Elcio
E
Santos
Luis A
LA
Boschi
Anselmo O
AO
Raddi
Maria Stella G
MS
D002130:Calcium Phosphates / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003738:Dental Cements / Q000633:toxicity*; D005347:Fibroblasts; D006801:Humans; D018675:Toxicity Tests / Q000379:methods*
D002130:Calcium Phosphates; D003738:Dental Cements