Study of oral tolerance and its indirect effects in adoptive cell transfer experiments.
Parenteral exposure to antigens to which oral tolerance had been previously induced results in the inhibition of immune responses to other unrelated antigens. Herein we tested whether indirect effects of oral tolerance could be adoptively transferred. Anti-Ova- and antihemoglobin-specific responsiveness as well as oral tolernace to Ova were transferred to irradiated, but not to normal, nonirradiated recepients. Irradiation, thus, facilitated adoptive transfer of oral tolerance. However, the inhibitory (indirect) effects upon the unrelated immunogen were not adoptively transferred, even to irradiated recepients. In addition, we studied adoptively transferred CFSE-labeled spleen cells by flow cytometry in recipient spleen, inguinal lymph nodes, and bone marrow, both in irradiated and nonirradiated recipients, 1, 3, or 5 days after cell transfer. Comparing the percent and absolute number of CFSE-labeled cells in each organ displayed significant differences in the dynamics of decay of adoptively transferred cells from tolerant or immune donors.
da Cunha
André Pires
AP
Laboratório de Imunobiologia, Departamento de Bioquímica e Imunologia, ICB-UFMG, Av. Antônio Carlos, 6627 Pampulha, Belo Horizonte, MG, Brazil.
Vaz
Nelson Monteiro
NM
Carvalho
Claudia Rocha
CR
D000284:Administration, Oral; D019264:Adoptive Transfer; D000818:Animals; D000917:Antibody Formation; D001853:Bone Marrow / Q000276:immunology; D006454:Hemoglobins / Q000276:immunology*; D007108:Immune Tolerance; D018928:Immunity, Mucosal / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D006454:Hemoglobins; D010047:Ovalbumin