Physicochemical characterization of casein phosphopeptide-amorphous calcium phosphate nanocomplexes.
Milk caseins stabilize calcium and phosphate ions and make them available to the neonate. Tryptic digestion of the caseins yields phosphopeptides from their polar N-terminal regions that contain clusters of phosphorylated seryl residues. These phosphoseryl clusters have been hypothesized to be responsible for the interaction between the caseins and calcium phosphate that lead to the formation of casein micelles. The casein phosphopeptides stabilize calcium and phosphate ions through the formation of complexes. The calcium phosphate in these complexes is biologically available for intestinal absorption and remineralization of subsurface lesions in tooth enamel. We have studied the structure of the complexes formed by the casein phosphopeptides with calcium phosphate using a range of physicochemical techniques including x-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, and equilibrium binding analyses. The amorphous nature of the calcium phosphate phase was confirmed by two independent methods: x-ray powder diffraction and selected area diffraction. In solution, the ion activity product of a basic amorphous calcium phosphate phase was the only ion product that was a function of bound phosphate independent of pH, consistent with basic amorphous calcium phosphate being the phase stabilized by the casein phosphopeptides. Detailed investigations of calcium and calcium phosphate binding using a library of synthetic homologues and analogues of the casein phosphopeptides have revealed that although the fully phosphorylated seryl-cluster motif is pivotal for the interaction with calcium and phosphate, other factors are also important. In particular, calcium binding and calcium phosphate stabilization by the peptides was influenced by peptide net charge, length, and sequence.
Cross
Keith J
KJ
Centre for Oral Health Science, School of Dental Science, The University of Melbourne, Melbourne, Victoria 3010, Australia.
Huq
N Laila
NL
Palamara
Joseph E
JE
Perich
John W
JW
Reynolds
Eric C
EC
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D002364:Caseins / Q000737:chemistry; D015723:Gene Library; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D008823:Micelles; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008892:Milk; D010455:Peptides / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry; D011485:Protein Binding; D012694:Serine / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; D002364:Caseins; D007477:Ions; D008823:Micelles; D010455:Peptides; D010710:Phosphates; D010750:Phosphoproteins; C519480:amorphous calcium phosphate; D012694:Serine; C020243:calcium phosphate; D014357:Trypsin; D002118:Calcium