[Altered colonic transit in TNBS-induced experimental colitis in guinea pig and distribution of nitric oxide synthase in the colonic wall].
OBJECTIVE
Inflammation-induced alterations in smooth muscle contractility may be due to the effects on smooth muscle itself, neurotransmitters or enteric nerves. In dextran sulfate sodium-induced colitic rat, the delay in colonic transit was caused by decreased activity and production of neuronal nitric oxide synthase (nNOS) in the myenteric plexus of the distal colon. The aim of this study was to investigate the relationship between the delay in colonic transit and the distribution of inducible NOS (iNOS) and nNOS immunoreactive cells in the myenteric plexus of trinitrobenzene sulfonic acid (TNBS)-induced colitic guinea pig.


METHODS
Sacrificed and their colonic tissues of forty-five TNBS-induced colitic guinea pigs were used to measure the colonic transit, and analyzed by immunohistochemistry.


RESULTS
Colonic transit was delayed significantly at 3, 7 and 14 days after administration of TNBS. In control, nNOS immunoreactivity was present in the mucosa, submucosa, lamina propria, and ganglion cells of the myenteric plexus, while after TNBS treatment, reduced nNOS cells were found. However, the number of nNOS ganglion cells in the myenteric plexus was similar to those seen in controls. After administration of TNBS, iNOS immunoreactivity was increased in the mucosa and submucosa, but the number of iNOS positive ganglion cells in the myenteric plexus was not changed compared to control.


CONCLUSIONS
It is suggested that in TNBS-induced guinea pig colitis, delayed colonic transit is not associated with the expression of nNOS nor iNOS in the myenteric plexus.
Cho
Seung Hyun
SH
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Park
Hyojin
H
Chung
Jun Pyo
JP
Lee
Young Ho
YH
Ji
Sang Won
SW
No
Tae Woong
TW
Lee
Sang In
SI
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology*; D003106:Colon / Q000201:enzymology* / Q000294:innervation; D005772:Gastrointestinal Transit; D006168:Guinea Pigs; D008297:Male; D009197:Myenteric Plexus / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D014302:Trinitrobenzenesulfonic Acid
D014302:Trinitrobenzenesulfonic Acid; D019001:Nitric Oxide Synthase