Citricoccus alkalitolerans sp. nov., a novel actinobacterium isolated from a desert soil in Egypt.
An actinobacterium, strain YIM 70010T, which was isolated from a desert soil sample collected in Egypt, was subjected to a polyphasic taxonomy study. The organism was alkalitolerant and its optimum growth occurred at pH 8.0-9.0. The isolate contained chemotaxonomic markers that were characteristic of the genus Citricoccus, i.e. the peptidoglycan type Lys-Gly-Glu (variation A4alpha), the predominant menaquinone MK-9(H2) and a polar lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unknown glycolipids. The major fatty acids were anteiso-C(15 : 0) and iso-C(15 : 0). The G+C content of the genomic DNA was 63.8 mol%. Strain YIM 70010T exhibited a 16S rRNA gene sequence similarity of 99.6 % and DNA-DNA relatedness value of 56 % with Citricoccus muralis DSM 14442T. The phenotypic characteristics and DNA-DNA relatedness data indicate that strain YIM 70010T can be distinguished from C. muralis (DSM 14442T). Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus Citricoccus, Citricoccus alkalitolerans sp. nov. (type strain, YIM 70010)=CCTCC AA 203008T=DSM 15665T=KCTC 19012T) is proposed.
Li
Wen-Jun
WJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.|The Key Laboratory for Microbial Resources of Ministry of Education, P. R. China, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Chen
Hua-Hong
HH
Department of Chemistry, Chuxiong Normal College, Chuxiong, Yunnan, 675000, P. R. China.|The Key Laboratory for Microbial Resources of Ministry of Education, P. R. China, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Zhang
Yu-Qin
YQ
The Key Laboratory for Microbial Resources of Ministry of Education, P. R. China, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Kim
Chang-Jin
CJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Park
Dong-Jin
DJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Jae-Chan
JC
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of Ministry of Education, P. R. China, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Jiang
Cheng-Lin
CL
The Key Laboratory for Microbial Resources of Ministry of Education, P. R. China, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
D015373:Bacterial Typing Techniques; D016227:Benzoquinones / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003889:Desert Climate; D004534:Egypt; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D008835:Micrococcaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D016227:Benzoquinones; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008055:Lipids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; C004532:quinone