Transfection activity of polyamidoamine dendrimers having hydrophobic amino acid residues in the periphery.
We designed poly(amidoamine) dendrimers with phenylalanine or leucine residues at their chain ends. Thereby, we achieved efficient gene transfection of cells through synergy of the proton sponge effect, which is induced by the internal tertiary amines of the dendrimer, and hydrophobic interaction by the hydrophobic amino acid residues in the dendrimer periphery. Dendrimers having 16, 29, 46, and 64 terminal phenylalanine residues were prepared by the reaction of the amine-terminated poly(amidoamine) G4 dendrimer and L-phenylalanine using condensing reagent 1,3-dicyclohexylcarbodiimide. Transfection activity of these phenylalanine-modified dendrimers for CV1 cells, an African green monkey kidney cell line, increased concomitant with the increasing number of the terminal phenylalanine residues, except for the dendrimer with 64 phenylalanine residues, which showed poor water solubility and hardly formed a complex with DNA at neutral pH. However, under weakly acidic conditions, the dendrimer with 64 phenylalanine residues formed a complex with DNA, thereby achieving highly efficient transfection. In contrast, the attachment of L-leucine residues was unable to improve the transfection activity of the parent dendrimer, probably because of the relatively lower hydrophobicity of this amino acid. The phenylalanine-modified dendrimer exhibited a higher transfection activity and a lower cytotoxicity than some widely used transfection reagents. For that reason, the phenylalanine-modified dendrimers are considered to be promising gene carriers.
Kono
Kenji
K
Department of Applied Materials Science, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan. kono@ams.osakafu-u.ac.jp
Akiyama
Hitoshi
H
Takahashi
Toshinari
T
Takagishi
Toru
T
Harada
Atsushi
A
D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry; D018014:Gene Transfer Techniques; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007668:Kidney / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D010649:Phenylalanine / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology; D018675:Toxicity Tests; D014162:Transfection / Q000379:methods*
D000596:Amino Acids; D011073:Polyamines; D010649:Phenylalanine; D004247:DNA; D008156:Luciferases