Immunohistochemical localization of cyclooxygenase isoforms in the organ of Corti and the spiral ganglion cells of guinea pig cochlea.
Prostaglandins have been used in experimental models and clinical studies for the therapy of sudden hearing loss and tinnitus with conflicting results. However, little is known about the rate-limiting enzymes of prostaglandin synthesis in the inner ear, the generally constitutively expressed cyclooxygenase 1 (COX-1) and the distress-inducible cyclooxygenase 2 (COX-2). To extend our knowledge concerning the physiological expression and localization of these two enzymes, immunohistochemical stainings of the guinea pig cochlea were performed. Light microscopical analysis revealed a homogenous distribution of COX-1 within nearly all cell types of the organ of Corti, but no COX-1 expression in the cuticular plates of pillar cells. COX-2 was found to be expressed in all cell types, with much stronger expression in Hensen cells, neighboring Deiters cells and cuticular plates of outer hair cells. Both COX-1 and COX-2 immunoreactions were also found in the spiral ganglion. We conclude that both COX subtypes are expressed in the guinea pig cochlea under physiological conditions. The prominent expression of the distress-inducible COX-2 isoform in cell types under mechanical stress during noise reception might support the hypothesis of a cytoprotective function of COX products in hearing and in cellular stress situations like intense noise exposure.
Ziegler
E A
EA
Department of Otorhinolaryngology, Head and Neck Surgery, Johannes Gutenberg University Medical School, Mainz, Germany.
Brieger
J
J
Heinrich
U R
UR
Mann
W J
WJ
D000818:Animals; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D004195:Disease Models, Animal; D006168:Guinea Pigs; D003639:Hearing Loss, Sudden / Q000188:drug therapy / Q000201:enzymology; D007150:Immunohistochemistry / Q000379:methods*; D009925:Organ of Corti / Q000187:drug effects / Q000201:enzymology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D011465:Prostaglandins, Synthetic / Q000378:metabolism / Q000627:therapeutic use; D013136:Spiral Ganglion / Q000187:drug effects / Q000201:enzymology*; D014012:Tinnitus / Q000188:drug therapy / Q000201:enzymology
D011465:Prostaglandins, Synthetic; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases