title: Isolation of recombinant antibodies (scFvs) to grapevine virus B.
abstract: A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
authors.affiliation: Istituto di Virologia Vegetale del CNR, Sezione di Bari, Italy. csvvps04@area.ba.cnr.it
authors.forename: P
authors.initials: P
authors.lastname: Saldarelli
authors.forename: H
authors.initials: H
authors.lastname: Keller
authors.forename: M
authors.initials: M
authors.lastname: Dell'Orco
authors.forename: A
authors.initials: A
authors.lastname: Schots
authors.forename: V
authors.initials: V
authors.lastname: Elicio
authors.forename: A
authors.initials: A
authors.lastname: Minafra
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D007128:Immunoglobulin Fragments / Q000276:immunology / Q000302:isolation & purification*; D019151:Peptide Library; D010942:Plant Viruses / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification*; D027843:Vitis / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; C055966:immunoglobulin Fv