Cleavage of integrin by mu-calpain during hypoxia in human endometrial cells.
OBJECTIVE
The distribution and activation of mu-calpain and possible cleavage of integrin in human endometrial cells under hypoxic condition were investigated.


METHODS
Human endometrial epithelial and stromal cells were subjected to hypoxia, and subsequently used for immunostaining and western blot analysis.


RESULTS
The proform of mu-calpain was detected in the cytoplasm of normal cells, and displayed a substantial decrease after hypoxia. Conversely, the active form of mu-calpain was not detected in normal cells, but was abundant after hypoxia. The cytoplasmic domain of integrin beta3 was also detected in the cytoplasm of endometrial cells. Western blot analysis confirmed that both the proform of mu-calpain and the integrin beta3 cytoplasmic domain decreased during hypoxia.


CONCLUSIONS
Mu-calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin beta3 cytoplasmic domain may give some adverse effects to the function of human endometrium.
Aoyama
Kazufumi
K
Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan. k-aoyama@xd5.so-net.ne.jp
Ozaki
Yasuhiko
Y
Nakanishi
Tamao
T
Ogasawara
Mayumi S
MS
Ikuta
Katsuo
K
Aoki
Koji
K
Blomgren
Klas
K
Suzumori
Kaoru
K
D015153:Blotting, Western; D002154:Calpain / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D039661:Integrin beta3 / Q000378:metabolism*; D018613:Microscopy, Confocal
D039661:Integrin beta3; D002154:Calpain; C449002:mu-calpain