[Determination of carvacrol and thymol in Mosla chinensis by HPLC].
OBJECTIVE
To establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis.


METHODS
The sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm.


RESULTS
The linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%.


CONCLUSIONS
The method is reliable, and can be used for quality control of M. chinensis.
Ji
Li
L
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Wang
Fang
F
Liu
Yuan-yan
YY
Tong
Yan
Y
Li
Xian-duan
XD
Feng
Xue-feng
XF
Huang
Lu-qi
LQ
Zhou
Guo-ping
GP
D002851:Chromatography, High Pressure Liquid; D000081007:Cymenes; D019686:Lamiaceae / Q000737:chemistry*; D039821:Monoterpenes / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013943:Thymol / Q000032:analysis*
D000081007:Cymenes; D039821:Monoterpenes; D013943:Thymol; C073316:carvacrol