title: Improvement of the Aspergillus oryzae enolase promoter (P-enoA) by the introduction of cis-element repeats.
abstract: We constructed a protein expression vector with an improved enoA promoter that harbored 12 tandem repeats of the cis-acting element (region III) of Aspergillus oryzae. The improved promoter yielded reporter beta-glucuronidase (GUS) activity approximately 30-fold of the original promoter. Northern blot analysis confirmed that GUS expression was increased at the transcriptional level. The transformant harboring seven copies of the novel vector showed more than 100,000 U/mg GUS protein, which was approximately 30% of all the cell-free soluble proteins.
authors.affiliation: General Research Laboratory, Ozeki Corporation, Hyogo, Japan. hirokazu.tsuboi@ozeki.co.jp
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tsuboi
authors.forename: Akio
authors.initials: A
authors.lastname: Koda
authors.forename: Tomomi
authors.initials: T
authors.lastname: Toda
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Minetoki
authors.forename: Masato
authors.initials: M
authors.lastname: Hirotsune
authors.forename: Masayuki
authors.initials: M
authors.lastname: Machida
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020080:Tandem Repeat Sequences
chemical_list: D005966:Glucuronidase; D010751:Phosphopyruvate Hydratase