title: Cathepsin B and tumor proteolysis: contribution of the tumor microenvironment.
abstract: Tumor-stromal interactions induce expression of matrix metalloproteinases and serine proteases and, as shown recently, the cysteine protease cathepsin B. We speculate that such interactions upregulate the transcription factor Ets1, resulting in increased cathepsin B expression. This would be consistent with the observed concomitant upregulation of matrix metalloproteinases and serine proteases as well as with the ability of extracellular matrices and their binding partners to alter cathepsin B expression and secretion. Using a confocal assay to analyze the contribution of tumor-stromal interactions to proteolysis, we have been able to confirm enhanced degradation of extracellular matrices by all three classes of proteases.
authors.affiliation: Department of Pharmacology, Wayne State University, Detroit, MI 48201, USA. bsloane@med.wayne.edu
authors.forename: Bonnie F
authors.initials: BF
authors.lastname: Sloane
authors.forename: Shiqing
authors.initials: S
authors.lastname: Yan
authors.forename: Izabela
authors.initials: I
authors.lastname: Podgorski
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Linebaugh
authors.forename: Michael L
authors.initials: ML
authors.lastname: Cher
authors.forename: Jianxin
authors.initials: J
authors.lastname: Mai
authors.forename: Dora
authors.initials: D
authors.lastname: Cavallo-Medved
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Sameni
authors.forename: Julie
authors.initials: J
authors.lastname: Dosescu
authors.forename: Kamiar
authors.initials: K
authors.lastname: Moin
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000032:analysis / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000201:enzymology* / Q000473:pathology; D017154:Stromal Cells / Q000502:physiology
chemical_list: D002401:Cathepsin B