Pseudocohnilembus persalinus (Ciliophora: Scuticociitida) is an additional species causing scuticociliatosis in olive flounder Paralichthys olivaceus.
In the present study, Pseudocohnilembus persalinus was first reported as a species causing scuticociliatosis in olive flounder Paralichthys olivaceus. Based on the stained specimens, P. persalinus was clearly differentiated from Uronema marinum, which has been shown to be a cause of scuticociliatosis in farmed olive flounder in Korea from its characteristic oral infraciliature structure. The 1754 bp small subunit ribosomal RNA (SS rRNA) gene sequence of P. persalinus showed 95% homology with the partial sequence of P. hargisi SS rRNA. Moreover, multiplex PCR based on the species-specific amplification of the SS rRNA gene sequence enabled us to distinguish P. persalinus from U. marinum in a simple and rapid manner. P. persalinus was clearly differentiated from U. marinum even when the host was infected simultaneously with both species. These data suggest that the multiplex PCR procedure would make it possible to avoid the cumbersome and time-consuming procedures of morphological analysis for the definitive identification of ciliates.
Kim
Sung Mi
SM
Department of Aquatic Life Medicine, Pukyong National University, Pusan 608-737, Korea.
Cho
Jae Bum
JB
Lee
Eun Hye
EH
Kwon
Se Ryun
SR
Kim
Sung Koo
SK
Nam
Yoon Kwon
YK
Kim
Ki Hong
KH
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000469:parasitology; D016798:Ciliophora / Q000166:cytology* / Q000235:genetics*; D016770:Ciliophora Infections / Q000453:epidemiology / Q000662:veterinary*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005393:Fish Diseases / Q000453:epidemiology* / Q000469:parasitology; D005432:Flounder; D005880:Gills / Q000469:parasitology; D006652:Histological Techniques; D007723:Korea / Q000453:epidemiology; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012867:Skin / Q000469:parasitology; D013045:Species Specificity
D017931:DNA Primers; D012335:RNA, Ribosomal