Inhibition of neoplastic transformation of benzo[alpha]pyrene-treated BALB/c 3T3 murine cells by a phytochemical extract of passionfruit juice.
The phytochemical composition of passionfruit juice (PFJ) was hypothesized to have valuable anti-cancer activity, and this was tested in a BALB/c 3T3 neoplastic transformation model. A higher concentration of PFJ compared with a lower concentration was effective in reducing the number, size, and invasiveness of transformed foci. When incubated with another mammalian cell line, the MOLT-4, PFJ was unable to alter the cell cycle kinetics while at the same time was successful in inducing the activity of caspase-3, an enzyme that commits the cell to apoptosis. This suggests that phytochemicals found in PFJ were able to produce the changes in transformed foci due to apoptotic mechanisms rather than by a reduction in cell proliferation. These beneficial results were achieved at levels that could theoretically be attained in the plasma after consumption of the juice.
Rowe
Cheryl A
CA
Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA.
Nantz
Meri P
MP
Deniera
Cindy
C
Green
Kristen
K
Talcott
Stephen T
ST
Percival
Susan S
SS
D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001564:Benzo(a)pyrene / Q000633:toxicity; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000517:prevention & control*; D029598:Passiflora / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D010936:Plant Extracts; D001564:Benzo(a)pyrene; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases