title: Production of recombinant human tenascin-C module containing a cell adhesion recognition motif of RGD.
abstract: Recombinant human tenascin peptide (hTNCIII3) that includes the Arg-Gly-Asp (RGD) cell recognition site was expressed in Escherichia coli using a prokaryotic expression system. Addition of recombinant hTNCIII3 peptide enhanced cell adhesion and survival of human chondrocytes by about 3-fold in each case.
authors.affiliation: Department of Biochemistry, Inha University College of Medicine, Incheon 400-712, Korea. juhjang@inha.ac.kr
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Hwang
authors.forename: Chong-Pyoung
authors.initials: CP
authors.lastname: Chung
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008422:Materials Testing; D009842:Oligopeptides / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000494:pharmacology; D019063:Tenascin / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D019063:Tenascin; C047981:arginyl-glycyl-aspartic acid