title: [Study on tissue culture of Ficus hirta].
abstract: The tissue culture of Ficus hirta Vahl. was studied. The nodes were used as explants and 1/2MS media with different plant growth regulators were tested. The result showed that the adventitious bud differentiation medium was 1/2MS + BA1.0 mg/L + NAA1.0 mg/L and 1/2MS + BA1.5 mg/L + NAA0.5 mg/L, the media for multiplication was 1/2MS +6 - BA0.5 mg/L, and the medium for rooting was 1/2MS + IBA1 mg/L. The cause of nigrescence in the tissue culture and its preventive methods were also discussed in this paper.
authors.affiliation: Zhongkai Agrotechnical College, Guangzhou.
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Huang
authors.forename: Wanfeng
authors.initials: W
authors.lastname: Zhang
authors.forename: Honghua
authors.initials: H
authors.lastname: Xu
authors.forename: Chunling
authors.initials: C
authors.lastname: Liu
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology; D003470:Culture Media; D030681:Ficus / Q000254:growth & development* / Q000502:physiology; D008027:Light; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development* / Q000502:physiology; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D001205:Ascorbic Acid