Metal phosphonates applied to biotechnologies: a novel approach to oligonucleotide microarrays.
A new process for preparing oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well-defined interface. Oligonucleotide probes terminated with phosphate are spotted directly on to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. The mixed organic/inorganic thin films have also been extended for use arraying DNA duplex probes, and therefore represent a viable general approach to DNA-based bioarrays. Ideas for interfacing mixed organic/inorganic interfaces to other bioapplications are also discussed.
Bujoli
Bruno
B
Laboratoire de Synthèse Organique, UMR CNRS 6513 & FR CNRS 2465, 2 Rue de la Houssinière, BP92208, 44322 Nantes Cedex 03, France. bujoli@chimie.univ-nantes.fr
Lane
Sarah M
SM
Nonglaton
Guillaume
G
Pipelier
Muriel
M
Léger
Jean
J
Talham
Daniel R
DR
Tellier
Charles
C
D001665:Binding Sites; D004247:DNA / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry*
D015345:Oligonucleotide Probes; D009942:Organometallic Compounds; D063065:Organophosphonates; D004247:DNA; D015040:Zirconium