Effects of bile acids on proliferation and production of proteinase activity of Uronema marinum (Ciliophora: Scuticociliatida).
Little is known about the effects of bile acids in relation to infectivity on the biological characteristics of Uronema marinum, a serious opportunistic parasite of farmed olive flounder Paralichthys olivaceus. In this study, we examined the effects of bile acids on the proliferation of U. marinum and on proteinase production in vitro. Proliferation of U, marinum was significantly enhanced by lithocholic acid (LCA) at 30 and 60 pmol, and by chenodeoxycholic acid (CDCA) at 0.06 pmol. In contrast, a significant decrease in proliferation was observed with cholic acid (CA) at 30 and 60 micromol, and with deoxycholic acid (DCA) at all amounts used. Proteinase production from live U. marinum was significantly increased by LCA, whereas CA significantly decreased proteinase production. CDCA and DCA had no effect on proteinase production. Although the types and concentrations of bile acids in the faeces of olive flounder are not known, the present results suggest that bile acids in the culturing water might influence the proliferation and production of proteinases in U. marinum, resulting in an increased possibility of scuticociliatosis in olive flounder farms.
Kim
Hyoung Jun
HJ
Department of Aquatic Life Medicine, Pukyong National University, Pusan 608-737, South Korea.
Choi
Su Kyung
SK
Choi
Seung Hyuk
SH
Kim
Min Sun
MS
Kim
Sung Koo
SK
Nam
Yoon Kwon
YK
Kim
Ki Hong
KH
D000818:Animals; D017756:Aquaculture; D001647:Bile Acids and Salts / Q000633:toxicity*; D002635:Chenodeoxycholic Acid / Q000494:pharmacology; D019826:Cholic Acid / Q000633:toxicity; D016798:Ciliophora / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D016770:Ciliophora Infections / Q000662:veterinary*; D003840:Deoxycholic Acid / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005393:Fish Diseases / Q000469:parasitology*; D005432:Flounder; D008095:Lithocholic Acid / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000378:metabolism*; D018709:Statistics, Nonparametric
D001647:Bile Acids and Salts; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D008095:Lithocholic Acid; D010447:Peptide Hydrolases; D019826:Cholic Acid