title: Mapping and functional characterization of the TAF11 interaction with TFIIA.
abstract: TFIIA interacts with TFIID via association with TATA binding protein (TBP) and TBP-associated factor 11 (TAF11). We previously identified a mutation in the small subunit of TFIIA (toa2-I27K) that is defective for interaction with TAF11. To further explore the functional link between TFIIA and TAF11, the toa2-I27K allele was utilized in a genetic screen to isolate compensatory mutants in TAF11. Analysis of these compensatory mutants revealed that the interaction between TAF11 and TFIIA involves two distinct regions of TAF11: the highly conserved histone fold domain and the N-terminal region. Cells expressing a TAF11 allele defective for interaction with TFIIA exhibit conditional growth phenotypes and defects in transcription. Moreover, TAF11 imparts changes to both TFIIA-DNA and TBP-DNA contacts in the context of promoter DNA. These alterations appear to enhance the formation and stabilization of the TFIIA-TBP-DNA complex. Taken together, these studies provide essential information regarding the molecular organization of the TAF11-TFIIA interaction and define a mechanistic role for this association in the regulation of gene expression in vivo.
authors.affiliation: Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Robinson
authors.forename: G
authors.initials: G
authors.lastname: Yatherajam
authors.forename: R T
authors.initials: RT
authors.lastname: Ranallo
authors.forename: A
authors.initials: A
authors.lastname: Bric
authors.forename: M R
authors.initials: MR
authors.lastname: Paule
authors.forename: L A
authors.initials: LA
authors.lastname: Stargell
mesh_terms: D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D035561:Transcription Factor TFIIA / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C105463:TAF11 protein, S cerevisiae; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein; D035561:Transcription Factor TFIIA; D035362:Transcription Factor TFIID