Differential regulation of nuclear receptors, neuropeptides and peptide hormones in the hypothalamus and pituitary of food restricted rats.
Food restriction is associated with a number of endocrine disturbances. We validated the experimental conditions for several house-keeping genes and determined the effects of 12 day 50% food restriction on hypothalamic and pituitary transcription of genes involved in different neuroendocrine systems, using real-time quantitative polymerase chain reaction (PCR). A total of 7 nuclear receptors and 12 neuropeptides and peptide hormones were investigated in the dorsal and ventral hypothalamus and the pituitary gland in rats. In the hypothalamus, food restriction reduced mRNA levels of estrogen receptor alpha (ERalpha), progesterone receptor, glucocorticoid receptor, thyroid hormone receptor alpha and beta, pro-opiomelanocortin (POMC), growth hormone-releasing factor (GHRF), corticotropin-releasing factor (CRF), thyrotropin-releasing factor (TRF), somatostatin, and increased that of neuropeptide Y (NPY). In the pituitary, the treatment reduced growth hormone (GH), luteinizing hormone beta (LHbeta) and thyrotropin beta, but increased ERalpha mRNA levels. The study provides a map of how food restriction affects the regulation of a number of transcripts involved in neuroendocrine control.
Lindblom
Jonas
J
Department of Neuroscience, Division of Pharmacology, Box 593 BMC, S-751 24 Uppsala, Sweden. Jonas.Lindblom@neuro.uu.se
Haitina
Tatjana
T
Fredriksson
Robert
R
Schiöth
Helgi B
HB
D000818:Animals; D001835:Body Weight / Q000502:physiology; D018076:DNA, Complementary / Q000096:biosynthesis; D004435:Eating / Q000502:physiology; D005508:Food Deprivation / Q000502:physiology*; D005786:Gene Expression Regulation; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000378:metabolism*; D036361:Peptide Hormones / Q000378:metabolism*; D010913:Pituitary-Adrenal System / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D018076:DNA, Complementary; D009479:Neuropeptides; D036361:Peptide Hormones; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D012313:RNA