title: Protective effect of prenatal water restriction on offspring cardiovascular homeostasis in response to hemorrhage.
abstract: We determined the cardiovascular and AVP responses of prenatally dehydrated (PreDehy) neonates to intravascular hemorrhage. Ewes with singleton fetuses were subjected to water restriction from 110 days of gestation to full term to achieve hypernatremia of 8-10 meq/l. Water and food were provided ad libitum to control ewes. After delivery, water and food were provided ad libitum to ewes from both groups, and newborns were allowed to nurse ad libitum. At 15 +/- 2 days of age, PreDehy and control lambs were prepared with bladder and femoral catheters and studied at 25 +/- 2 days of age. After a 2-h basal period, lambs were hemorrhaged to 30% of blood volume over 1 h (0.5% of blood volume/min) and monitored 1 h after hemorrhage. Neonatal arterial blood pressure was measured, and blood samples were collected. Basal plasma sodium levels, plasma osmolality, hematocrit, and mean arterial pressure were increased in PreDehy lambs compared with controls. Both groups had similar basal AVP levels and heart rate. In response to hemorrhage, all parameters remained significantly elevated in PreDehy lambs. Blood pressure decreased less in PreDehy lambs than in controls. The hemorrhage-AVP threshold (percent blood volume withdrawal at which plasma AVP values significantly increased) was markedly elevated (20 vs. 15%) and peak hemorrhage-induced AVP plasma levels were lower (5.6 +/- 1.5 vs. 10.1 +/- 1.5 pg/ml, P < 0.01) in PreDehy lambs than in controls. Thus offspring of dehydrated ewes demonstrate enhanced AVP secretory responses to hypotension. Despite potential long-term adverse effects of systemic hypertension, these results suggest a protective effect of prenatal water restriction on offspring cardiovascular homeostasis during blood volume reduction.
authors.affiliation: Dept. of Obstetrics and Gynecology, LABioMed at Harbor-UCLA Medical Center, 1124 West Carson St. Box 446, RB-1 Bldg., Torrance, CA 90502, USA. mdesai@obgyn.humc.edu
authors.forename: Mina
authors.initials: M
authors.lastname: Desai
authors.forename: Catalina
authors.initials: C
authors.lastname: Guerra
authors.forename: Shengbiao
authors.initials: S
authors.lastname: Wang
authors.forename: Michael G
authors.initials: MG
authors.lastname: Ross
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001810:Blood Volume; D002319:Cardiovascular System / Q000196:embryology*; D003681:Dehydration / Q000503:physiopathology; D005260:Female; D005865:Gestational Age; D005919:Glomerular Filtration Rate; D006470:Hemorrhage / Q000196:embryology / Q000517:prevention & control*; D006706:Homeostasis; D006801:Humans; D006955:Hypernatremia / Q000196:embryology*; D011247:Pregnancy; D012756:Sheep; D014868:Water Deprivation / Q000502:physiology*