title: High-performance liquid chromatography with ultraviolet detection for therapeutic drug monitoring of everolimus.
abstract: We developed and validated a high-performance liquid chromatography-ultraviolet (HPLC-UV) method for determining everolimus concentrations in human whole blood. Sample preparation involved a solid-phase extraction after protein precipitation. The separation of everolimus from internal standard (IS) and endogenous components was achieved using an isocratic elution on an octyl column. The method showed a linear relationship between peak height ratios and blood concentrations in the range of 1-200 ng/mL (r(2)=0.9997). The observed intra- and inter-day assay imprecision had a coefficient of variation (CV)=12.8%, and inaccuracy was 11.4%. The method was found to be precise, accurate, and sensible making it useful for routine therapeutic monitoring of everolimus.
authors.affiliation: Department of Medicine and Transplantation, Ospedali Riuniti di Bergamo-Mario Negri Institute for Pharmacological Research, Via Gavazzeni 11, 24125 Bergamo, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Baldelli
authors.forename: Stefano
authors.initials: S
authors.lastname: Murgia
authors.forename: Simona
authors.initials: S
authors.lastname: Merlini
authors.forename: Stefania
authors.initials: S
authors.lastname: Zenoni
authors.forename: Norberto
authors.initials: N
authors.lastname: Perico
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Remuzzi
authors.forename: Dario
authors.initials: D
authors.lastname: Cattaneo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D004355:Drug Stability; D000068338:Everolimus; D016027:Heart Transplantation; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood* / Q000627:therapeutic use; D008297:Male; D012680:Sensitivity and Specificity; D020123:Sirolimus / Q000031:analogs & derivatives* / Q000097:blood* / Q000627:therapeutic use; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007166:Immunosuppressive Agents; D000068338:Everolimus; D020123:Sirolimus