title: Using 2H2O to study the influence of feeding on protein synthesis: effect of isotope equilibration in vivo vs. in cell culture.
abstract: We previously reported that 2H2O can be used to measure rates of protein synthesis during prolonged steady-state conditions (Previs SF, Fatica R, Chandramouli V, Alexander JC, Brunengraber H, and Landau BR. Am J Physiol Endocrinol Metab 286: E665-E672, 2004). The underlying premise of our method is that following the administration of 2H2O, 2H atoms in body water rapidly equilibrate with free alanine before it is incorporated into newly synthesized proteins. We have now directly examined whether 2H2O can be used to measure the influence of a single meal on protein synthesis. In addition, we have compared the use of 2H2O for measuring rates of protein synthesis in vivo vs. in cell culture. Using a rat model, we observed rapid equilibration between 2H in body water and free alanine; therefore we were able to study the response of protein synthesis to a single meal. We observed that approximately 50% of the plasma albumin that is synthesized over the course of 24 h is made within approximately 5 h after eating (in rats trained to eat a complete 24-h ration of food in a single meal). Contrary to what we observed in vivo, feeding (the replenishment of cell culture medium) does influence the use of 2H2O for in vitro studies. In particular, since there can be slow equilibration of 2H between water and alanine in the cell culture medium, special consideration must be made to avoid underestimating the rate of protein synthesis in vitro.
authors.affiliation: Dept. of Nutrition, D-201, CWRU School of Medicine, Cleveland, OH 44106, USA.
authors.forename: Danielle A
authors.initials: DA
authors.lastname: Dufner
authors.forename: Ilya R
authors.initials: IR
authors.lastname: Bederman
authors.forename: Daniel Z
authors.initials: DZ
authors.lastname: Brunengraber
authors.forename: Nadia
authors.initials: N
authors.lastname: Rachdaoui
authors.forename: Faramarz
authors.initials: F
authors.lastname: Ismail-Beigi
authors.forename: Brett A
authors.initials: BA
authors.lastname: Siegfried
authors.forename: Scot R
authors.initials: SR
authors.lastname: Kimball
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Previs
mesh_terms: D000409:Alanine / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D017666:Deuterium Oxide / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D010750:Phosphoproteins / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D038601:Ribosomal Protein S6 / Q000378:metabolism; D019893:Ribosomal Protein S6 Kinases / Q000378:metabolism; D012709:Serum Albumin / Q000096:biosynthesis*
chemical_list: D002352:Carrier Proteins; C499526:Eif4ebp1 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D038601:Ribosomal Protein S6; D012709:Serum Albumin; D019893:Ribosomal Protein S6 Kinases; D017666:Deuterium Oxide; D000409:Alanine