title: Effects of lactoferrin, phytic acid, and EDTA on oxidation in two food emulsions enriched with long-chain polyunsaturated fatty acids.
abstract: The influence of the addition of metal chelators on oxidative stability was studied in a milk drink and in a mayonnaise system containing highly polyunsaturated lipids. Milk drinks containing 5% (w/w) of specific structured lipid were supplemented with lactoferrin (6-24 muM) and stored at 2 degrees C for up to 9 weeks. Mayonnaise samples with 16% fish oil and 64% rapeseed oil (w/w) were supplemented with either lactoferrin (8-32 muM), phytic acid (16-124 muM), or EDTA (16-64 muM) and were stored at 20 degrees C for up to 4 weeks. The effect of the metal chelators was evaluated by determination of peroxide values, secondary volatile oxidation products, and sensory analysis. Lactoferrin reduced the oxidation when added in concentrations of 12 muM in the milk drink and 8 muM in the mayonnaise, whereas it was a prooxidant at higher concentrations in both systems. In mayonnaise, EDTA was an effective metal chelator even at 16 muM, whereas phytic acid did not exert a distinct protective effect against oxidation. The differences in the equimolar effects of the metal chelators are proposed to be due to differences in their binding constants to iron and their different stabilities toward heat and low pH.
authors.affiliation: Department of Seafood Research, Danish Institute for Fisheries Research, and BioCentrum-DTU, Building 221, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. nsn@dfu.min.dk
authors.forename: Nina S
authors.initials: NS
authors.lastname: Nielsen
authors.forename: Arni
authors.initials: A
authors.lastname: Petersen
authors.forename: Anne S
authors.initials: AS
authors.lastname: Meyer
authors.forename: Maike
authors.initials: M
authors.lastname: Timm-Heinrich
authors.forename: Charlotte
authors.initials: C
authors.lastname: Jacobsen
mesh_terms: D000818:Animals; D001628:Beverages / Q000032:analysis; D004492:Edetic Acid / Q000008:administration & dosage*; D004655:Emulsions / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D005395:Fish Oils; D005524:Food Technology; D005527:Food, Fortified / Q000032:analysis*; D007781:Lactoferrin / Q000008:administration & dosage*; D008892:Milk / Q000737:chemistry; D010084:Oxidation-Reduction; D010833:Phytic Acid / Q000008:administration & dosage*; D010938:Plant Oils; D000074262:Rapeseed Oil; D012677:Sensation
chemical_list: D004655:Emulsions; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D010938:Plant Oils; D000074262:Rapeseed Oil; D010833:Phytic Acid; D004492:Edetic Acid; D007781:Lactoferrin