Expression of a cephalosporin C esterase gene in Acremonium chrysogenum for the direct production of deacetylcephalosporin C.
A recombinant fungal microorganism capable of producing deacetylcephalosporin C was constructed by transforming a cephalosporin C esterase gene from Rhodosporidium toruloides into Acremonium chrysogenum. The cephalosporin C esterase gene can be expressed from its endogenous R. toruloides promoter or from the Aspergillus nidulans trpC promoter under standard Acremonium chrysogenum fermentation conditions. The expression of an active cephalosporin C esterase enzyme in A. chrysogenum results in the conversion of cephalosporin C to deacetylcephalosporin C in vivo, a novel fermentation process for the production of deacetylcephalosporin C. The stability of deacetylcephalosporin C in the fermentation broth results in a 40% increase in the cephalosporin nucleus.
Basch
J
J
Analytical Biochemistry, Technical Operations, Bristol-Myers Squibb Company, Syracuse, NY 13221-4755, USA.
Franceschini
T
T
Tonzi
S
S
Chiang
S-J D
SJ
D000164:Acremonium / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D002511:Cephalosporins / Q000378:metabolism*; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal
D002511:Cephalosporins; C025071:deacetylcephalosporin C; D002265:Carboxylic Ester Hydrolases; C019861:cephalosporin-C deacetylase