title: Modulation of an outer membrane protease contributes to the virulence defect of Shigella flexneri strains carrying a mutation in the virK locus.
abstract: The Shigella actin assembly protein IcsA is removed from the bacterial surface by the protease IcsP. We show that decreased intracellular spreading of virK::Tn10 mutants is due in part to significant increases in IcsP and IcsP-mediated cleavage of IcsA and that IcsP expression is a critical determinant of Shigella virulence.
authors.affiliation: Bacterial Pathogenesis Laboratories, University Park, 65 Landsdowne Street, Cambridge, MA 02139, USA.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Wing
authors.forename: Seth R
authors.initials: SR
authors.lastname: Goldman
authors.forename: Shabeen
authors.initials: S
authors.lastname: Ally
authors.forename: Marcia B
authors.initials: MB
authors.lastname: Goldberg
mesh_terms: D000199:Actins / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D002465:Cell Movement / Q000502:physiology; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism*; D012763:Shigella flexneri / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D000199:Actins; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D010447:Peptide Hydrolases; C060971:SopA protein, Bacteria