Expression of AT1-R in marmoset whole adrenal glands and adrenocortical cells in culture.
The marmoset adrenal is interesting as it is developmentally similar to humans and other primates, but in adulthood 17,20-lyase activity is very low. One possible explanation is an altered regulation of P450c17 expression by AT1-R. We investigated the expression and zonal distribution of the AT1-R in marmoset adrenal glands and adrenocortical cells in culture since it is known that AT1-R is a regulator of P450c17 expression and activity. AT1-R was expressed strongly in the ZG and to diminishing degrees through the remainder of the cortex. There was negative expression in a putative ZI. Dispersed adrenocortical cells retained AT1-R protein as detected by ICC. Cells cultured for several days and treated in serum-free media for 48 h maintained AT1-R expression, as measured by western. However, at that 48 h time point, treatment with Forsk, AII, TPA, or the combination of A+F or T+F did not appear to effect AT1-R expression. We conclude that marmoset adrenals express AT1-R similarly to humans and other higher mammals, adrenocortical cells retain AT1-R expression in culture, and consistent with other adrenal cell culture models, AT1-R expression is not lost in response to agonists long term.
Bird
Ian M
IM
Perinatal Research Labs, Department Ob/Gyn, University of Wisconsin, 7E Meriter Hospital/Park, 202 South Park St., Madison, WI 53715, USA. imbird@wisc.edu
Pattison
J Christina
JC
D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002144:Callithrix / Q000378:metabolism*; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D004338:Drug Combinations; D007150:Immunohistochemistry / Q000379:methods; D008253:Macaca mulatta; D008297:Male; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*; D013194:Staining and Labeling; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014018:Tissue Distribution; D015384:Zona Glomerulosa / Q000378:metabolism
D004338:Drug Combinations; D044140:Receptor, Angiotensin, Type 1; D000804:Angiotensin II; D005576:Colforsin; D013254:Steroid 17-alpha-Hydroxylase; D013755:Tetradecanoylphorbol Acetate