title: Origins of mouse inbred strains deduced from whole-genome scanning by polymorphic microsatellite loci.
abstract: Microsatellite loci are uniformly distributed at approximately 100-kbp intervals on all chromosomes except the chromosome Y, and genetic information about more than 9000 loci and high-throughput polymorphism analysis are now available. Taking advantage of these properties, we carried out whole-genome scanning using eight common inbred strains (CIS) of laboratory mice, including A/J, C57BL/6J, CBA/J, DBA/2J, SM/J, SWR/J, NC/Nga, and 129/SvJ, and eight wild-derived inbred strains (WIS), BGL2/Ms, CAST/Ei, JF1/Ms, MSM/Ms, NJL/Ms, PGN2/Ms, SK/CamEi, and SWN/Ms. We selected and located 1226 informative loci at 1.2-cM average intervals on all of the chromosomes of the 16 strains and compared the polymorphisms of the eight CIS with those from the eight WIS as subspecies representatives. More than 50% of the loci can be identified as WIS (therefore, subspecies-specific) alleles in the CIS genomes. We also discovered that the CIS chromosomes form a mosaic structure with an average ratio of domesticus to non-domesticus alleles of 3:1. Furthermore, the domesticus alleles were present much more frequently on the CIS chromosome X than on their autosomes, suggesting that successive backcrossing of non-domesticus stocks to domesticus stocks had been undergone at the beginning of CIS history.
authors.affiliation: Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), Tokyo, 113-8613, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sakai
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kikkawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Miura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Kazuo
authors.initials: K
authors.lastname: Moriwaki
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Shiroishi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Satta
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Takahata
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yonekawa
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D016678:Genome; D023281:Genomics / Q000379:methods; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic
chemical_list: D004272:DNA, Mitochondrial