Effects of functional group position on spatial representations of aliphatic odorants in the rat olfactory bulb.
Principles of olfactory coding can be clarified by studying the olfactory bulb activity patterns that are evoked by odorants differing systematically in chemical structure. In the present study, we used series of aliphatic esters, ketones, and alcohols (27 odorants total) to determine the effects of functional group position on glomerular-layer activity patterns. These patterns were measured as uptake of [(14)C]2-deoxyglucose and were mapped into standardized data matrices for statistical comparison across different rats. The magnitude of the effect of position differed greatly for the different functional groups. For ketones, there was little or no effect of position on evoked patterns. For esters, uptake in individual glomerular modules increased, whereas uptake in others decreased with changing group position, yet the overall patterns remained similar. For alcohols, group position had a profound effect on evoked activity patterns. For example, moving the hydroxyl group in either heptanol or nonanol from the first to the fourth carbon changed the activity patterns so greatly that the major areas of response did not overlap. Within every functional group series, however, responses were globally chemotopic, such that pairs of odorants with the smallest difference in functional group position evoked the most similar patterns. These results help to define further the specificities of glomeruli within previously described glomerular modules, and they show that functional group position can be an important feature in encoding an odorant molecule.
Johnson
Brett A
BA
Department of Neurobiology and Behavior, University of California, Irvine, Irvine, CA 92697-4550, USA. bajohnso@uci.edu
Farahbod
Haleh
H
Saber
Sepideh
S
Leon
Michael
M
D000438:Alcohols / Q000737:chemistry; D000818:Animals; D000831:Animals, Newborn; D001931:Brain Mapping; D002250:Carbon Radioisotopes; D003847:Deoxyglucose / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D007659:Ketones / Q000737:chemistry; D008968:Molecular Conformation; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009812:Odorants; D009830:Olfactory Bulb / Q000033:anatomy & histology* / Q000187:drug effects / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000187:drug effects / Q000502:physiology*; D009833:Olfactory Pathways / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D018035:Receptors, Odorant / Q000502:physiology; D012903:Smell / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology
D000438:Alcohols; D002250:Carbon Radioisotopes; D004952:Esters; D007659:Ketones; D018035:Receptors, Odorant; D003847:Deoxyglucose