title: Validation of intraoperative diagnoses using smear preparations from stereotactic brain biopsies: intraoperative versus final diagnosis--influence of clinical factors.
abstract: OBJECTIVE
  Despite improvements in imaging techniques, histopathological diagnosis is still an important tool in neuro-oncology. At Freiburg University Hospital in Germany, approximately 450 patients per year undergo a serial stereotactic biopsy to obtain a diagnosis. We analyzed the accuracy of intraoperative diagnosis for rapid establishment of treatment options. Furthermore, we wanted to find out whether the location and histopathology of the tumors as well as the age and sex of the patients affected accuracy. Because of the large number of biopsies performed per year, parameters could also be evaluated for rare cerebral lesions.
  
  
  METHODS
  We retrospectively analyzed 5000 consecutive stereotactic brain biopsies from 4589 patients. The digital database comprises the intraoperative and final diagnoses, the location of the tumors, and the sex and age of the patients. Regression analysis was performed to identify parameters that had a significant impact on the results.
  
  
  RESULTS
  Intraoperative diagnosis was correct in 90.3% of biopsies. This included complete correlation in 81.3% of the biopsies and partial correlation in 9% of the biopsies. In 5.1% of the biopsies, no correlation between the intraoperative and final diagnosis was obtained. In 4.6% of the biopsies, no diagnosis could be made during or after surgery. A high correlation was found for World Health Organization Type II astrocytomas and, with regression analysis, for World Health Organization Type I astrocytomas, glioblastomas, and metastases.
  
  
  CONCLUSIONS
  Intraoperative diagnosis with stereotactic biopsy has high validity. Immediate treatment based on the intraoperative diagnosis can be justified (e.g., for metastases or glioblastomas). Stereotactic biopsy with an exact histopathological diagnosis is strongly recommended for planning adequate therapy for patients with unidentified brain lesions.
authors.affiliation: Abteilung Neuropathologie, Universität Freiburg, Freiburg, Germany. tilgner@nz.ukl.uni-freiburg.de
authors.forename: Johannes
authors.initials: J
authors.lastname: Tilgner
authors.forename: Manfred
authors.initials: M
authors.lastname: Herr
authors.forename: Christoph
authors.initials: C
authors.lastname: Ostertag
authors.forename: Benedikt
authors.initials: B
authors.lastname: Volk
mesh_terms: D001706:Biopsy / Q000379:methods*; D001932:Brain Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007432:Intraoperative Period; D008297:Male; D016634:Radiosurgery; D015203:Reproducibility of Results; D012189:Retrospective Studies