Receptor editing in peripheral B cell tolerance.
Receptor editing or secondary Ig gene rearrangement occurs in immature, autoreactive B cells to maintain self-tolerance. Here we show that nonspontaneously autoimmune mice immunized with a peptide mimetope of DNA develop peptide- and DNA-reactive antibodies. Antigen-specific B cells display a follicular B cell phenotype. As these cells move into the memory compartment, many express RAG protein and acquire expression of both kappa and lambda light chains. Thus, this study provides evidence for receptor editing occurring in a mature, antigen-activated B cell population. Because the receptor editing observed here occurred in an autoreactive response to antigen, it may function to maintain peripheral tolerance.
Rice
Jeffrey S
JS
Department of Microbiology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Newman
Jeffrey
J
Wang
Chuansheng
C
Michael
Daniel J
DJ
Diamond
Betty
B
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000276:immunology; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics; D007108:Immune Tolerance / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology
D004268:DNA-Binding Proteins; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2; D049452:Green Fluorescent Proteins