title: Healthy girl born after cryopreservation of gametes and ICSI in a patient with seminoma.
abstract: This study reports birth in a case of intracytoplasmic sperm injection with cryopreserved oocytes and spermatozoa banked after radiotherapy and prior to chemotherapy due the occurrence of two non-synchronous seminomas. A 30-year-old male with a diagnosis of seminoma cryopreserved six vials of spermatozoa. After oncological treatment was completed, his partner, a 24-year-old woman, underwent ovarian stimulation. Seventeen oocytes were retrieved; one was at the germinal vesicle stage and two were injected, resulting in two embryos. Fourteen metaphase II oocytes were frozen. The woman presented moderated ovarian hyperstimulation syndrome, and embryo cryopreservation was indicated. After endometrial preparation, two embryos were transferred and a pregnancy was achieved. The woman suffered eclampsia during week 28 of gestation. Caesarean section was performed and a preterm girl weighing 1000 g was born, but died 2 weeks after delivery. A year later, a second procedure was begun. Frozen oocytes and one vial of semen were thawed. Eight of the 14 oocytes survived and were microinjected; two became fertilized and one good quality cleaved embryo was transferred. Pregnancy was achieved and a healthy girl was born with a birth weight of 2800 g. Oocyte cryopreservation associated with sperm banking in cancer patients is a useful tool for couples seeking deferred parenthood.
authors.affiliation: Department of Reproductive Medicine, Cer Medical Institute, Humboldt 2263 (1425), Buenos Aires, Argentina. cermed@satlink.com
authors.forename: Judith
authors.initials: J
authors.lastname: Notrica
authors.forename: Andrea
authors.initials: A
authors.lastname: Divita
authors.forename: Fernando
authors.initials: F
authors.lastname: Neuspiller
authors.forename: Gabriela
authors.initials: G
authors.lastname: Arenas
authors.forename: Ester Polak
authors.initials: EP
authors.lastname: de Fried
mesh_terms: D000328:Adult; D015925:Cryopreservation; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D011256:Pregnancy Outcome; D012662:Semen Preservation; D018239:Seminoma / Q000628:therapy; D020554:Sperm Injections, Intracytoplasmic; D013736:Testicular Neoplasms / Q000628:therapy; D014015:Tissue Banks; D016896:Treatment Outcome