title: Multi-slice CT for visualization of acute pulmonary embolism: single breath-hold subtraction technique.
abstract: OBJECTIVE
  The purpose of our preliminary animal study was to evaluate the feasibility of a new subtraction technique for visualization of perfusion defects within the lung parenchyma in segmental and subsegmental pulmonary embolism (PE).
  
  
  METHODS
  In three healthy pigs, PE were artificially induced by fresh human clot material. Within a single breath-hold, CT angiography (CTA) was performed on a 16-slice multi-slice CT scanner (SOMATOM Sensation 16; Siemens, Forchheim, Germany) before and after intravenous application of 80 mL of contrast-medium, followed by a saline chaser. Scan parameters were 120 kV and 100 mAs (eff.), using a collimation of 16 x 1.5 mm and a table speed/rot. of 36 mm (pitch: 1.5; rotation time: 0.5 s). A new 3D subtraction technique was developed, which is based on automated segmentation, non-linear spatial filtering and non-rigid registration. Data were analysed using a color-encoded "compound view" of parenchymal enhancement and CTA information displayed in axial, coronal and sagittal orientation.
  
  
  RESULTS
  Subtraction was technically feasible in all three data sets. The mean scan time for each series was 4.7 s, interscan delay was 14.7 s, respectively. Therefore, an average breath-hold of approximately 24 s was required for the overall scanning procedure. Downstream of occluded segmental and subsegmental arteries, perfusion defects were clearly assessable, showing lower or missing enhancement compared to normally perfused lung parenchyma. In all pigs, additional peripheral areas with triangular shaped perfusion defects were delineated, considered typical for PE.
  
  
  CONCLUSIONS
  Our initial results from the animal model studied show that perfusion imaging of PE is feasible within a single breath-hold. It allows a comprehensive assessment of perfusion deficits as the direct proof of a pulmonary embolus, can be combined with an indirect visual quantification of the density changes in the adjacent lung tissue.
authors.affiliation: Department of Diagnostic Radiology, University of Technology, Aachen, Germany. wildberg@rad.rwth-aachen.de
authors.forename: J E
authors.initials: JE
authors.lastname: Wildberger
authors.forename: E
authors.initials: E
authors.lastname: Klotz
authors.forename: H
authors.initials: H
authors.lastname: Ditt
authors.forename: A H
authors.initials: AH
authors.lastname: Mahnken
authors.forename: E
authors.initials: E
authors.lastname: Spüntrup
authors.forename: R W
authors.initials: RW
authors.lastname: Günther
mesh_terms: D000208:Acute Disease; D000792:Angiography; D000818:Animals; D004195:Disease Models, Animal; D005240:Feasibility Studies; D011655:Pulmonary Embolism / Q000000981:diagnostic imaging*; D013382:Subtraction Technique; D013552:Swine; D036542:Tomography, Spiral Computed; D014057:Tomography, X-Ray Computed / Q000379:methods*