title: The implantation of Taenia solium metacestodes in mice induces down-modulation of T-cell proliferation and cytokine production.
abstract: The purpose of this study was to determine the effect of the implantation of Taenia solium metacestodes and the treatment with suppressive metacestode factor (F1) on the ability of spleen cells from Balb/c mice to produce cytokines. Cytokine production was estimated 12 days following the implantation or 4 days after the last dose of F1 (five doses) by RT-PCR and flow cytometry analyses. Spleen cells were obtained from metacestode-implanted, F1-treated and control mice. They were stimulated with concanavalin A (ConA) ex vivo and used for RT-PCR studies and for CD25 expression and intracellular cytokine production estimations using specific monoclonal antibodies labeled with phycoerithrin or fluorescein. Results of the RT-PCR showed that all cells expressed IFN-gamma, IL-2 and IL-4 mRNAs. IL-10 mRNA was not expressed in any case. Flow cytometry analyses showed that both spleen CD4+ and CD8+ cells from metacestode-implanted or treated-F1 mice expressed significantly diminished percentages of CD25 when compared with control cells (P<0.05). The estimation of intracellular cytokines showed that the production of IL-2 and IL-4 in CD8+ cells, and of IFN-gamma in CD4+ cells from mice implanted with metacestodes was significantly impaired when compared with the values from control cells (P<0.05).
authors.affiliation: Servicio de Hematología, Hospital General de México OD, México City, Mexico.
authors.forename: Lilian
authors.initials: L
authors.lastname: Hernández-Mendoza
authors.forename: José Luis
authors.initials: JL
authors.lastname: Molinari
authors.forename: Esperanza
authors.initials: E
authors.lastname: Garrido
authors.forename: Isabel
authors.initials: I
authors.lastname: Cortés
authors.forename: Sandra
authors.initials: S
authors.lastname: Solano
authors.forename: Enrique
authors.initials: E
authors.lastname: Miranda
authors.forename: Patricia
authors.initials: P
authors.lastname: Tato
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003551:Cysticercosis / Q000276:immunology*; D003552:Cysticercus / Q000276:immunology; D016207:Cytokines / Q000032:analysis* / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D041201:Taenia solium / Q000276:immunology*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2