title: Preparation and evaluation of uniform-sized molecularly imprinted polymer beads used for the separation of sulfamethazine.
abstract: Uniform-sized molecularly imprinted polymer (MIP) beads were prepared using a one-step swelling and polymerization method. The obtained sulfamethazine (SMZ)-imprinted polymer showed high affinity and selectivity toward SMZ and other structurally related sulfonamides in acetonitrile or water-acetonitrile mobile phases, particularly in high aqueous systems. The column performance of the MIPs for SMZ and its analogues could be improved by elevating the column temperature and optimizing the flow rate. The hydrogen-bonding effect plays a significant role in the recognition process of SMZ-imprinted polymer systems in organic media, while the ion-exchange effect, as well as hydrophobic effect, dominates the retention mechanism in aqueous-rich media, in addition to shape recognition.
authors.affiliation: Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Dihua
authors.initials: D
authors.lastname: Shangguan
authors.forename: Guoquan
authors.initials: G
authors.lastname: Liu
mesh_terms: D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007474:Ion Exchange; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013418:Sulfamethazine / Q000302:isolation & purification*; D013449:Sulfonamides / Q000737:chemistry; D013696:Temperature; D014867:Water
chemical_list: D000097:Acetonitriles; D011137:Polystyrenes; D013449:Sulfonamides; D014867:Water; D013418:Sulfamethazine; C032159:acetonitrile