Production of monoclonal antibody for the detection of meat and bone meal in animal feed.
For the detection of prohibited meat and bone meal (MBM) in animal feed, monoclonal antibodies (MAbs) were raised against heat-stable h-caldesmon purified from bovine intestinal smooth muscle. The obtained hybridoma cells were screened against extracts of the bovine MBM and heat-treated smooth muscle, and MAb 5E12 was identified as having the best performance. Antibody 5E12 did not react with animal feed, milk product, plant proteins, and other ingredients used for commercial animal feed except for the gelatin. This antibody diluted to 100-fold was able to detect MBM mixed in animal feed at 0.05% in an ELISA, and it showed strong affinity toward bovine smooth muscle autoclaved at 130 degrees C. Therefore, this antibody can be used in the ELISA system for field testing of the presence of MBM in animal feed.
Kim
Shin-Hee
SH
Department of Nutritional Sciences, College of Human Environmental Sciences, Oklahoma State University, Stillwater, OK 74078, USA. kimshin@okstate.edu
Huang
Tung-Shi
TS
Seymour
Thomas A
TA
Wei
Cheng-i
CI
Kempf
Stephen C
SC
Bridgman
C Roger
CR
Clemens
Roger A
RA
An
Haejung
H
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001688:Biological Products; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D006358:Hot Temperature; D006825:Hybridomas / Q000276:immunology; D007422:Intestines / Q000276:immunology; D008460:Meat / Q000032:analysis*; D051379:Mice; D008903:Minerals / Q000032:analysis*; D009130:Muscle, Smooth / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D001688:Biological Products; D008903:Minerals; C027762:bone meal