Two-photon excitation induced fluorescence of a trifluorophore-labeled DNA.
Two-photon excitation of a trifluorophore (6-carboxyfluorescein, N,N,N',N'-tetramethyl-6-carboxyrhodamine and cyanine-5 monofunctional dye) labeled DNA, which has a scaffold of 26 nucleotides, was achieved using focused laser light of a Q-switched Nd-YAG laser (1064 nm). The observed fluorescence signature (emission ratio from the three fluorophores) of the labeled DNA after two-photon excitation is very different from the fluorescence signatures produced by one-photon excitation at different wavelength. The additional fluorescence signatures produced by two-photon excitation of the fluorescent oligonucleotides will facilitate their use as combinatorial fluorescence energy transfer tags for multiplex genetic analysis.
Jockusch
Steffen
S
Department of Chemistry, Columbia University, New York, NY 10027, USA.
Li
Zengmin
Z
Ju
Jingyue
J
Turro
Nicholas J
NJ
D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D015394:Molecular Structure; D017785:Photons
D005456:Fluorescent Dyes; D004247:DNA