Determination of chemical agent optical clearing potential using in vitro human skin.
OBJECTIVE
Optical clearing is a method that temporarily reduces the optical scattering of biological tissues.


METHODS
To determine the optical clearing potential (OCP) of various chemical agents, we examined the change in reduced scattering coefficients of in vitro human skin after application directly to the dermal side.


CONCLUSIONS
We found that the mean agent OCP did not correlate with refractive index or osmolality. Lipophilic hyperosmotic agents had a mean OCP less than unity, indicating increased optical scattering.
Choi
Bernard
B
Beckman Laser Institute, University of California, Irvine, California 92612, USA. bchoi@laser.bli.uci.edu
Tsu
Laura
L
Chen
Eric
E
Ishak
Teresa S
TS
Iskandar
Sarah M
SM
Chess
Samuel
S
Nelson
J Stuart
JS
D046508:Culture Techniques; D006801:Humans; D009930:Organic Chemicals / Q000494:pharmacology*; D012016:Reference Values; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry*; D012869:Skin Absorption / Q000502:physiology*; D012879:Skin Physiological Phenomena; D041623:Tomography, Optical Coherence
D009930:Organic Chemicals