Beneficial effect of auto-aggregating Lactobacillus crispatus on experimentally induced colitis in mice.
We tested the therapeutic relevance of auto aggregation in lactobacilli by comparing the effect on DSS induced colitis of viable Lactobacillus crispatus M247, isolated from healthy humans, to L. crispatus MU5, an isogenic spontaneous mutants of M247, the latter lacking the auto aggregation phenotype which allows the adhesion to human mucus. Aggregating L. crispatus M247, but not the non-aggregating MU5, was retrievable from mice feces and adherent to the colonic mucosa. Daily administration of L. crispatus M247, but not heat killed L. crispatus M247 or aggregation deficient L. crispatus MU5, dose-dependently reduced the severity of DSS colitis. Indeed, L. crispatus MU5 administered in a 30% sucrose solution, known to restore the aggregation phenotype, had a protective effect comparable to mice receiving L. crispatus M247. These results indicate that a surface-mediated property such as aggregation may play a pivotal role in the protective effects obtained by dietary supplementation with L. crispatus M247 during colitis.
Castagliuolo
Ignazio
I
School of Pharmacy, Department of Histology, University of Padua, Via A. Gabelli 63, Padua 35121 Padua, Italy. ignazio.castagliuolo@unipd.it
Galeazzi
Francesca
F
Ferrari
Susanna
S
Elli
Marina
M
Brun
Paola
P
Cavaggioni
Andrea
A
Tormen
Deborah
D
Sturniolo
Giacomo C
GC
Morelli
Lorenzo
L
Palù
Giorgio
G
D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D015212:Inflammatory Bowel Diseases / Q000628:therapy*; D007778:Lactobacillus / Q000254:growth & development / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000382:microbiology; D009093:Mucus / Q000382:microbiology; D009154:Mutation; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*
D016264:Dextran Sulfate