Total and nematode-specific IgE responses in intestinal lymph of genetically resistant and susceptible sheep during infection with Trichostrongylus colubriformis.
Total and antigen-specific IgE responses in afferent (AIL) and efferent (EIL) intestinal lymph of sheep with a nematode resistant (R) or susceptible (S) genotype during challenge infection with the intestinal nematode parasite Trichostrongylus colubriformis were examined. Within each sheep line, lambs with a nematode naive or nematode field-primed pre-challenge status were used. Total IgE level in AIL and EIL was dependent on nematode infection and was further influenced by genotype or the immune phenotype (nematode immune mean FEC+/-SDM=77+/-179 or non-immune mean FEC+/-SDM=4016+/-4318) of the animal. During T. colubriformis challenge immune animals had higher levels of total IgE in lymph than non-immune sheep, R line sheep had higher concentrations of total IgE than S line sheep, and field-primed animals had higher total IgE levels than nematode naive animals. Concentrations of total IgE were consistently higher in AIL than EIL or serum and were higher in lymph draining the proximal than the distal jejunum demonstrating that polyclonal IgE in AIL was largely derived from the intestinal mucosa of the anatomical compartment where the nematodes reside. The consistently higher concentration of total IgE in AIL was dependent on phenotype or genotype and in S genotype sheep also on the pre-challenge status. Concentrations of nematode specific IgE were significantly higher in EIL than AIL indicating a preference for the production of IgE reacting with excretory secretory products of the infective T. colubriformis larvae in the regional lymph node.
Pernthaner
A
A
AgResearch Limited, Wallaceville Animal Research Centre, PO Box 40063, Ward Street, Upper Hutt, New Zealand.
Shaw
R J
RJ
McNeill
M M
MM
Morrison
L
L
Hein
W R
WR
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005260:Female; D020022:Genetic Predisposition to Disease; D007073:Immunoglobulin E / Q000276:immunology*; D007411:Intestinal Diseases, Parasitic / Q000235:genetics / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D008196:Lymph / Q000276:immunology / Q000469:parasitology; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology*; D014253:Trichostrongylosis / Q000276:immunology* / Q000469:parasitology / Q000662:veterinary*; D014254:Trichostrongylus / Q000276:immunology*
D000909:Antibodies, Helminth; D007073:Immunoglobulin E