title: Biosynthesis of irregular diterpenes in Anisotome lyallii by head-to-head coupling of geranyl diphosphate.
abstract: Anisotomenes, bicyclic irregular diterpenes found in the genus Anisotome(family Apiaceae), are shown to be products of a unique biosynthetic pathway, involving head-to-head coupling of two geranyl diphosphate units. (13)C labelling studies with [1-(13)C]-glucose on plantlets of A. lyallii also revealed that the isoprene subunits were formed via the MEP pathway. The in vitro-cultured plant material also yielded a new irregular, linear diterpene alcohol, that shares the same biosynthetic pathway.
authors.affiliation: Plant Extracts Research Unit, New Zealand Institute for Crop & Food Research Limited, P. O. Box 56, Dunedin, New Zealand. vanklinkj@crop.cri.nz
authors.forename: John W
authors.initials: JW
authors.lastname: van Klink
authors.forename: Hans
authors.initials: H
authors.lastname: Becker
authors.forename: Nigel B
authors.initials: NB
authors.lastname: Perry
mesh_terms: D019661:Apiaceae / Q000737:chemistry / Q000378:metabolism*; D002247:Carbon Isotopes; D011756:Diphosphates / Q000737:chemistry / Q000378:metabolism*; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D008968:Molecular Conformation; D011522:Protons; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D002247:Carbon Isotopes; D011756:Diphosphates; D004224:Diterpenes; D011522:Protons; C511282:geranyl diphosphate; D005947:Glucose