title: Prognostic value of structural chromosomal rearrangements and small cell clones with high hyperdiploidy in children with acute lymphoblastic leukemia.
abstract: In this study, 107 children with acute lymphoblastic leukemia (ALL) were analysed for the presence of hyperdiploidy by cytogenetics and interphase fluorescence in situ hybridisation (I-FISH). Structural aberrations in hyperdiploid cells were investigated by multiple colour FISH (mFISH). Clones with high hyperdiploidy (>50 chromosomes) (HeH) were found in 46 patients (43%). In nine of these (20%), the abnormal clone was present in <20% of the total cell population. There was no significant difference in EFS between those patients with HeH in 2.5-20% or >20% of cells. Structural rearrangements in the HeH clone were found in 10 patients (22%). In this study, HeH karyotypes containing structural aberrations were an indication of a poor prognosis in childhood ALL.
authors.affiliation: Center of Oncocytogenetics, Institute of Clinical Biochemistry and laboratory diagnosis, General Faculty Hospital and 1st Faculty of Medicine of Charles University, 12808 Prague 2, Czech Republic. zuze@vfn.cz
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Zemanova
authors.forename: Kyra
authors.initials: K
authors.lastname: Michalova
authors.forename: Lenka
authors.initials: L
authors.lastname: Sindelarova
authors.forename: Petr
authors.initials: P
authors.lastname: Smisek
authors.forename: Jana
authors.initials: J
authors.lastname: Brezinova
authors.forename: Sarka
authors.initials: S
authors.lastname: Ransdorfova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vavra
authors.forename: Alena
authors.initials: A
authors.lastname: Dohnalova
authors.forename: Jan
authors.initials: J
authors.lastname: Stary
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification* / Q000235:genetics* / Q000401:mortality; D011379:Prognosis
chemical_list: D004273:DNA, Neoplasm