Identification of jasmonic acid and its methyl ester as gum-inducing factors in tulips.
The purpose of this study was to identify endogenous factors that induce gummosis and to show their role in gummosis in tulip (Tulipa gesneriana L. cv. Apeldoorn) stems. Using procedures to detect endogenous factors that induce gum in the stem of tulips, jasmonic acid (JA) and methyl jasmonate (JA-Me) were successfully identified using gas-liquid chromatography-mass spectrometry. Total amounts of JA and JA-Me designated as jasmonates in tulip stems were also estimated at about 70-80 ng/g fresh weight, using deuterium-labeled jasmonates as internal standards. The application of JA and JA-Me as lanolin pastes substantially induced gums in tulip stems with ethylene production. The application of ethephon, an ethylene-generating compound, however, induced no gummosis although it slightly affected jasmonate content in tulip stems. These results strongly suggest that JA and JA-Me are endogenous factors that induce gummosis in tulip stems.
Skrzypek
Edyta
E
College of Integrated Arts and Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
Miyamoto
Kensuke
K
Saniewski
Marian
M
Ueda
Junichi
J
D000085:Acetates / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000502:physiology*; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D031447:Tulipa / Q000187:drug effects / Q000378:metabolism*
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D011134:Polysaccharides; C011006:jasmonic acid; C072239:methyl jasmonate