Light induction of the carotenoid biosynthesis pathway in Blakeslea trispora.
A gene of Blakeslea trispora has been cloned by heterologous hybridization with the Mucor circinelloides crgA gene, a repressor of light-inducible carotenogenesis. This gene is the ortholog of the M. circinelloides crgA, since it was able to restore the wild-type phenotype of a null crgA mutant of M. circinelloides. The expression of B. trispora crgA gene is light-induced and photoadapted, as occurs for M. circinelloides crgA. Light induction and photoadaptation of B. trispora crgA was also observed in M. circinelloides, which suggests that the mechanisms involved in light regulation are basically conserved between these filamentous fungi. Conservation of the regulatory pathway that controls carotene biosynthesis was supported by the light-induced and photoadapted expression of all structural carotenogenic genes of B. trispora. Consequently, the beta-carotene content of dark grown mycelia of B. trispora increased upon illumination with white light.
Quiles-Rosillo
María D
MD
Departamento de Genética y Microbiología , Facultad de Biología, Universidad de Murcia, 30071 Murcia, Spain.
Ruiz-Vázquez
Rosa M
RM
Torres-Martínez
Santiago
S
Garre
Victoriano
V
D000595:Amino Acid Sequence; D001483:Base Sequence; D002338:Carotenoids / Q000096:biosynthesis* / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D015870:Gene Expression / Q000528:radiation effects*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D008027:Light; D008969:Molecular Sequence Data; D009090:Mucorales / Q000235:genetics* / Q000502:physiology; D017434:Protein Structure, Tertiary; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C404790:CRGA protein, Mucor circinelloides; D005656:Fungal Proteins; D002338:Carotenoids