Micropatterning of cells using modulated magnetic fields.
A new technique of cell micropatterning was presented. Mouse osteoblast cells (MC3T3-E1) were seeded on a substrate whose surface was exposed to a periodically modulated magnetic field (a line pattern with a 200- or 600-microm pitch) produced by a field modulator inserted into a homogeneous magnetic field of 1 T generated by an electromagnet. The cells were trapped consistent with the line profile of the modulated field. The trapping efficiency was enhanced by adding Mn(II)EDTA (paramagnetic) to the cultivation medium. The cells were subsequently incubated in the magnetic field. The same technique was applied to whole blood to pattern red blood cells.
Kimura
Tsunehisa
T
Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo 192-0397, Japan. kimura-tsunehisa@c.metro-u.ac.jp
Sato
Yukiko
Y
Kimura
Fumiko
F
Iwasaka
Masakazu
M
Ueno
Shoogo
S
D016475:3T3 Cells; D000818:Animals; D018914:Cells, Immobilized; D004912:Erythrocytes; D006801:Humans; D008280:Magnetics; D051379:Mice