[Curcumin inhibits the proliferation of cultured human fetal retinal pigment epithelium cells].
OBJECTIVE
To investigate the effect of curcumin on the proliferation of human fetal retinal pigment epithelium (hfRPE) cells in vitro.


METHODS
Subcultured hfRPE cells were treated with different concentrations of curcumin (2.5, 5, 10, 20 microg/ml) for 24 or 48 hours. MTT assay and flow cytometry were performed to evaluate the effect of curcumin on the proliferation and cell cycle of cultured hfRPE cells.


RESULTS
Curcumin was effective in inhibiting the proliferation of subcultured hfRPE, the IC50 value at 24 and 48 hours were found to be 14.27 microg/ml, 12.7 microg/ml, respectively. Cell cycle analysis indicated that CUR blocked cells in G2/M phase.


CONCLUSIONS
Curcumin can inhibit the proliferation of hfRPE cells by cells cycle arrest.
Gong
Ling
L
Department of Ophthalmology, the 2nd Xiangya Hospital, Central South University, Changsha 410011, China. gl825@hotmail.com
Jiang
Deyong
D
Zhu
Xiaohua
X
Guo
Lihua
L
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D005333:Fetus; D006801:Humans; D010857:Pigment Epithelium of Eye / Q000166:cytology*
D000970:Antineoplastic Agents; D003474:Curcumin