Effects of oxidized LDL on in vitro proliferation and spontaneous motility of human coronary artery endothelial cells.
OBJECTIVE
To investigate the effects of low concentrations of oxidized LDL (oxLDL) on the proliferation and spontaneous motility of human coronary artery endothelial cells (HCAEC) in culture.


METHODS
Cultures of HCAEC were treated with low concentrations of native LDL (nLDL) isolated from human plasma and with LDL minimally oxidized through different chemical methods; the effects were compared.


RESULTS
Native LDL had no deleterious effects on in vitro proliferation and motility of HCAEC; however, at its highest concentration and for a longer exposure, nLDL inhibited cell proliferation. The LDL chemically oxidized by spermine nonoate (SNO) and 3-morpholinylsydnonimine (SYN-1) had significant inhibiting effects on in vitro proliferation and motility of HCAEC, which were proportional to the greatest concentrations and degrees of oxidation of LDL.


CONCLUSIONS
OxLDL has a cytotoxic effect, inhibiting the proliferation and spontaneous motility of HCAEC in culture. This effect is proportional to the concentration and degree of oxidation of LDL; native LDL is relatively innocuous.
Xavier
Hermes Toros
HT
Instituto do Coração, Faculdade de Medicina, USP, São Paulo, SP, Brazil. htxavier@cardiol.br
Abdalla
Dulcinéia Saes Parra
DS
Martinez
Tania Leme da Rocha
TL
Ramires
José Antonio Franchini
JA
Gagliardi
Antonio Ricardo de Toledo
AR
D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D003324:Coronary Artery Disease / Q000378:metabolism / Q000503:physiopathology; D003331:Coronary Vessels / Q000166:cytology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein