[Genetic analysis of cultivated ginseng population with the assistance of RAPD technology].
OBJECTIVE
To provide reference for breeding of cultivated ginseng by researching the genetic diversities among different strains.


METHODS
To make up the systematic diagram of genetic relationship by calculating the proximity coefficient with SPSS 10.0 software and clustered by between-groups linkage method.


RESULTS
The selected 18 random primers used in PCR amplification to produce 145 bands in 17 samples, among which 53 bands (36.5%) are polymorphic. The results of cluster analysis show that the genetic relationship between different strains of Da-maya is closer than that of Er-maya, which proves that it is feasible to differentiate the cultivated groups in molecular level on outside character.


CONCLUSIONS
RAPD is a favorable molecular marker to assist ginseng breeding.
Shao
Ai-juan
AJ
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Li
Xin
X
Huang
Lu-qi
LQ
Wei
Jian-he
JH
Lin
Shu-fang
SF
D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D014644:Genetic Variation; D005894:Panax / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D017931:DNA Primers; D018744:DNA, Plant