title: Trypanosoma cruzi infection disrupts vinculin costameres in cardiomyocytes.
abstract: Chagas' disease cardiomyopathy is an important manifestation of Trypanosoma cruzi infection, leading to cardiac dysfunction and serious arrhythmias. We have here investigated by indirect immunofluorescence assay the distribution of vinculin, a focal adhesion protein with a major role in the transmission of contraction force, during the T. cruzi-cardiomyocyte infection in vitro and in vivo. No change in vinculin distribution was observed after 24 h of infection, where control and T. cruzi-infected cardiomyocytes displayed vinculin localized at costameres and intercalated discs. On the other hand, a clear disruption of vinculin costameric distribution was noted after 72 h of infection. A significant reduction in the levels of vinculin expression was observed at all times of infection. In murine experimental Chagas' disease, alteration in the vinculin distribution was also detected in the infected myocardium, with no costameric staining in infected myocytes and irregular alignment of intercalated discs in cardiac fibers. These data suggest that the disruption of costameric vinculin distribution and the enlargement of interstitial space due to inflammatory infiltration may contribute to the reduction of transmission of cardiac contraction force, leading to alterations in the heart function in Chagas' disease.
authors.affiliation: Departamento de Ultra-estrutura e Biologia Celular, Laboratório de Ultra-estrutura Celular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Tatiana G
authors.initials: TG
authors.lastname: Melo
authors.forename: Danielle S
authors.initials: DS
authors.lastname: Almeida
authors.forename: Maria de Nazareth S L
authors.initials: Mde N
authors.lastname: de Meirelles
authors.forename: Mirian Claudia
authors.initials: MC
authors.lastname: Pereira
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D002598:Chagas Cardiomyopathy / Q000469:parasitology / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D018450:Disease Progression; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D006321:Heart / Q000469:parasitology; D006790:Host-Parasite Interactions; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000469:parasitology / Q000648:ultrastructure*; D009210:Myofibrils / Q000378:metabolism / Q000648:ultrastructure*; D014349:Trypanosoma cruzi / Q000472:pathogenicity; D016596:Vinculin / Q000378:metabolism*
chemical_list: D016596:Vinculin