Influence of temperature, salinity and pH on the growth of environmental Aeromonas and Vibrio species isolated from Mai Po and the Inner Deep Bay Nature Reserve Ramsar Site of Hong Kong.
Four environmental bacterial isolates including Aeromonas hydrophila MP-3, A. salmonicida MP-4, Vibrio vulnificus MP-2 and V. cholerae MP-1 isolated from sediment and water of Mai Po Nature Reserve of Hong Kong were examined for their responses to temperature, pH and salinity under laboratory conditions in this study. V. cholerae MP-1 was found to resist vibriostatic agent O/129 at concentration of 10 microg/ml. In addition, bacterial growth under test conditions was measured and the results were fitted into the Gompertz model to obtain important parameters related to bacterial growth, lag time (lambda), specific growth rate (mu(m)), and maximum biomass (A) for comparison. V. cholerae MP-1 did not show any apparent growth at 15 degrees C, but was adapted to a much wider environmental pH from 5.2 to 9.2 for growth while V. vulnificus MP-2 was more sensitive to pH changes yielding the highest biomass at pH 6.2. A. salmonicida MP-4 was surprisingly tolerant to salinity as high as 60.0 per thousand NaCl and grew almost equally well as under conditions of other treatments. All four bacterial isolates showed a wide spectrum of plasticity to the environmental conditions and they pose a potential threat to public health and animal health.
Wang
Yanling
Y
Laboratory of Environmental Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, P.R. China.
Gu
Ji-Dong
JD
D000333:Aeromonas / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D006723:Hong Kong; D006863:Hydrogen-Ion Concentration; D011621:Pteridines / Q000494:pharmacology; D012965:Sodium Chloride; D013696:Temperature; D014734:Vibrio cholerae / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D041261:Vibrio vulnificus / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D014871:Water Microbiology
D003470:Culture Media; D011621:Pteridines; C021594:O 129; D012965:Sodium Chloride