title: Activity of a paromomycin hydrophilic formulation for topical treatment of infections by Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis.
abstract: Studies on in vitro skin permeation and in vivo anti-leishmanial activity in mice experimentally infected with Leishmania (Leishmania) major pointed out to the potential of a new paromomycin (PA) formulation (hydrophilic gel) for treatment of cutaneous leishmaniasis (CL). In this study, the activity of this formulation was evaluated in animals experimentally infected by Leishmania species that prevail in the New World. PA gel activity was compared to antimony treatment, since it is still the first choice treatment to the different clinical forms of leishmaniasis. The topical treatment activity with 10% PA gel in BALB/c mice infected by Leishmania (Leishmania) amazonensis was higher than that observed for parenteral antimony treatment, while the efficacy of these two regimes in hamsters infected by Leishmania (Viannia) braziliensis was similar. These results suggest that this formulation could be suitable for clinical studies and may represent an alternative novel formulation for topical treatment of CL.
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Av Antônio Carlos, 6627, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Gisele Santos
authors.initials: GS
authors.lastname: Gonçalves
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Fernandes
authors.forename: Renata Celi Carvalho
authors.initials: RC
authors.lastname: Souza
authors.forename: Jarbas Eustáquio
authors.initials: JE
authors.lastname: Cardoso
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Oliveira-Silva
authors.forename: Fabiana Cristina
authors.initials: FC
authors.lastname: Maciel
authors.forename: Ana
authors.initials: A
authors.lastname: Rabello
authors.forename: Lucas Antônio Miranda
authors.initials: LA
authors.lastname: Ferreira
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000967:Antimony Sodium Gluconate / Q000494:pharmacology; D006224:Cricetinae; D005260:Female; D005782:Gels; D007273:Injections, Intramuscular; D007892:Leishmania braziliensis; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy* / Q000469:parasitology; D008297:Male; D008536:Meglumine / Q000494:pharmacology; D000077485:Meglumine Antimoniate; D051379:Mice; D008807:Mice, Inbred BALB C; D009942:Organometallic Compounds / Q000494:pharmacology; D010303:Paromomycin / Q000494:pharmacology*; D011897:Random Allocation; D018709:Statistics, Nonparametric
chemical_list: D005782:Gels; D009942:Organometallic Compounds; D010303:Paromomycin; D008536:Meglumine; D000077485:Meglumine Antimoniate; D000967:Antimony Sodium Gluconate