title: Free colorectal cancer cells on the peritoneal surface: correlation with pathologic variables and survival.
abstract: BACKGROUND
  Clinicopathologic staging of colorectal cancer remains the best predictor of survival. Prognostication for an individual with colorectal cancer remains elusive. This study was designed to investigate the incidence of free surface colorectal cancer cells detected by cytology during elective open curative resection, to correlate their presence with particular clinicopathologic variables and determine whether their presence was predictive of cancer-specific survival.
  
  
  METHODS
  Over a six-year period in one institution, all elective colon and intraperitoneal rectal cancer specimens were assessed during primary resection for the presence of free colorectal cancer cells by means of a simple and tested specimen imprint cytology methodology. Clinicopathologic variables were assessed prospectively and blinded to cytology results. All patients were followed up routinely until death and if the patient was not seen within the last six months, information was obtained from the New South Wales Registry of Births, Deaths and Marriages in Australia.
  
  
  RESULTS
  Overall, 26 of 281 (9.25 percent) colorectal cancers had positive cytology for cancer cells on the peritoneal surface of the bowel. Poorly differentiated tumors were significantly associated with positive cytology. Tumor penetration, presence of vascular or neural invasion, mucinous characteristics, lymph node status, and operative procedure performed were not statistically significant predictors of positive cytology. Overall, 43 of the 281 patients (15.3 percent) died during the mean follow-up period of 49.2 months from cancer-related deaths. Of these patients, 8 had positive cytology and 35 had negative cytology results. Cancer-specific survival assessed with the log-rank test was significantly associated with positive cytology in univariate (P = 0.008) and multivariate analysis (P < 0.001).
  
  
  CONCLUSIONS
  In this study, the presence of free surface colorectal cancer cells has been shown to be predictive of survival and is independent of direct peritoneal invasion and lymph node status. Thus, further assessment of this simple prognostic variable is warranted and selection of patients with positive cytology for possible adjuvant therapies may be beneficial.
authors.affiliation: Department of Colorectal Surgery and Surgical Outcomes, Research Center, Royal Prince Alfred Hospital, Sydney, Australia.
authors.forename: Sriskanthan
authors.initials: S
authors.lastname: Baskaranathan
authors.forename: Jeanette
authors.initials: J
authors.lastname: Philips
authors.forename: Pauline
authors.initials: P
authors.lastname: McCredden
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Solomon
mesh_terms: D000230:Adenocarcinoma / Q000401:mortality / Q000473:pathology / Q000601:surgery; D000704:Analysis of Variance; D002423:Cause of Death; D003585:Cell Biology / Q000592:standards*; D003082:Colectomy; D015179:Colorectal Neoplasms / Q000401:mortality / Q000473:pathology / Q000601:surgery; D003131:Combined Modality Therapy; D017558:Elective Surgical Procedures; D006801:Humans; D016343:Monitoring, Intraoperative / Q000379:methods / Q000592:standards*; D009364:Neoplasm Recurrence, Local / Q000209:etiology / Q000401:mortality; D009366:Neoplasm Seeding; D009367:Neoplasm Staging; D009517:New South Wales / Q000453:epidemiology; D018579:Patient Selection; D010537:Peritoneum; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D011446:Prospective Studies; D012042:Registries; D016019:Survival Analysis; D016896:Treatment Outcome