title: High-resolution blood-brain barrier permeability and blood volume imaging using quantitative synchrotron radiation computed tomography: study on an F98 rat brain glioma.
abstract: The authors previously provided evidence of synchrotron radiation computed tomography (SRCT) efficacy for quantitative in vivo brain perfusion measurements using monochromatic X-ray beams. However, this technique was limited for small-animal studies by partial volume effects. In this paper, high-resolution absolute cerebral blood volume and blood-brain barrier permeability coefficient measurements were obtained on a rat glioma model using SRCT and a CCD camera (47x47 microm2 pixel size). This is the first report of in vivo high-resolution brain vasculature parameter assessment. The work gives interesting perspectives to quantify brain hemodynamic changes accurately in healthy and pathological small animals.
authors.affiliation: Unité INSERM 647 RSRM (Rayonnement Synchrotron et Recherche Médicale), ID17-ESRF, Grenoble, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Adam
authors.forename: Christian
authors.initials: C
authors.lastname: Nemoz
authors.forename: Alberto
authors.initials: A
authors.lastname: Bravin
authors.forename: Stefan
authors.initials: S
authors.lastname: Fiedler
authors.forename: Sam
authors.initials: S
authors.lastname: Bayat
authors.forename: Sylvie
authors.initials: S
authors.lastname: Monfraix
authors.forename: Gilles
authors.initials: G
authors.lastname: Berruyer
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Charvet
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Le Bas
authors.forename: Hélène
authors.initials: H
authors.lastname: Elleaume
authors.forename: François
authors.initials: F
authors.lastname: Estève
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging*; D001921:Brain / Q000098:blood supply; D001932:Brain Neoplasms / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D002560:Cerebrovascular Circulation / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods; D005910:Glioma / Q000000981:diagnostic imaging*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D010539:Permeability; D051381:Rats; D017356:Synchrotrons; D014057:Tomography, X-Ray Computed / Q000379:methods*