title: Repetitive treatments of colon HT-29 cells with diallyl disulfide induce a prolonged hyperacetylation of histone H3 K14.
abstract: Diallyl disulfide (DADS) is a sulfur compound derived from garlic. Several studies carried out in rodents have revealed protective effects of DADS against colon carcinogenesis. The antipromoting effects of DADS may be partly related to its ability to inhibit tumoral cell proliferation. In a previous study, we have shown that in two human colon tumor cell lines (HT-29 and Caco-2) seeded at a low density (0.2 x 10(6) cells/100-mm petri dish), DADS antiproliferative effects were associated with a transient increase of histone H3 K14 acetylation. Moreover, DADS could inhibit nuclear histone deacetylase activity. Therefore, in the present study, we examined the possible effects of different experimental conditions (HT-29 cells at high density, repetitive treatments with DADS) on the pattern of DADS-induced histone hyperacetylation. Using HT-29 cells seeded at a higher density (5 x 10(6) cells/100-mm petri dish), we found that DADS induced histone H3 K14 hyperacetylation rapidly (3 h). When administrated as single treatments, the DADS effect on histone H3 K14 remained transient. In contrast, repetitive treatment with DADS resulted in a prolonged hyperacetylation of histone H3 K14. Whatever the cell culture conditions were, DADS had no effect on histone H4 acetylation. Thus, in vitro, the cell density and pattern of DADS treatment influenced the HT-29 nuclear response to DADS. DADS belongs to food-borne molecules that may play a role in chromatin remodeling and contribute to the nutritional modulation of gene expression.
authors.affiliation: Laboratoire de Nutrition et Sécurité Alimentaire, Institut National de al Recherche Agronomique, 78352 Jouy-en-Josas cedex, France. druesne@jouy.inra.fr
authors.forename: Nathalie
authors.initials: N
authors.lastname: Druesne
authors.forename: Anthony
authors.initials: A
authors.lastname: Pagniez
authors.forename: Camille
authors.initials: C
authors.lastname: Mayeur
authors.forename: Muriel
authors.initials: M
authors.lastname: Thomas
authors.forename: Claire
authors.initials: C
authors.lastname: Cherbuy
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Duée
authors.forename: Paule
authors.initials: P
authors.lastname: Martel
authors.forename: Catherine
authors.initials: C
authors.lastname: Chaumontet
mesh_terms: D000107:Acetylation; D000498:Allyl Compounds / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D004220:Disulfides / Q000494:pharmacology*; D019073:HT29 Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans
chemical_list: D000498:Allyl Compounds; D004220:Disulfides; D006657:Histones; C028009:diallyl disulfide