Presence of neuroglobin in cultured astrocytes.
Neuroglobin (Ngb), a recently discovered intracellular respiratory globin in neurons, may play a crucial role in oxygen homeostasis in the brain. We report preliminary findings indicating the presence of functional neuroglobin in primary cultures of cerebral cortical astrocytes. Reverse transcription real-time polymerase chain reaction (RRT-PCR) and immunostaining confirmed such presence in cultured astrocytes isolated from newborn mouse brain. Ngb antisense treatment increased apoptosis in ischemic astrocytes. The discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain.
Chen
Xiao Qian
XQ
Neuroscience Research Institute, Peking University, Key Laboratory of Neuroscience, Ministry of Education, Department of Neurobiology, Peking University Health Science Center, Beijing, People's Republic of China.
Qin
Lu Ye
LY
Zhang
Chen Gang
CG
Yang
Li Tao
LT
Gao
Zhen
Z
Liu
Shuang
S
Lau
Lok Ting
LT
Fung
Yin-Wan Wendy
YW
Greenberg
David A
DA
Yu
Albert Cheung-Hoi
AC
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D019556:COS Cells; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005914:Globins / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D000078787:Neuroglobin; D010101:Oxygen Consumption / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins