Liquid chromatography determination of residue levels on apples treated with cetylpyridinium chloride.
Cetylpyridinium chloride (CPC) has been found to be effective in reducing microbial contamination in apples. A sensitive and specific HPLC method was developed to determine CPC residues in apples treated with CPC. This method involves ion exchange solid-phase extraction, and the use of stearylpyridinium chloride (SPC) as internal standard. Limit of quantitation, was 0.5 microg/ml of CPC for the apple ethanolic extracts. The observed residues in apple (2.35-4.35 microg/g of apple) were lower than those previously reported for chicken and beef. The method is specific, sensitive, reproducible and accurate.
Rodríguez-Morales
Sergio
S
Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 4301 West Markham, Slot 522-3, Little Rock, AR 72205, USA.
Zhou
Xiang
X
Salari
Hamid
H
Castillo
Rafael
R
Breen
Philip J
PJ
Compadre
Cesar M
CM
D000890:Anti-Infective Agents / Q000032:analysis*; D002138:Calibration; D002594:Cetylpyridinium / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D027845:Malus / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000890:Anti-Infective Agents; D002594:Cetylpyridinium