Monitoring expression of cytochrome P450 genes during postischemic rat liver reperfusion using DNA microarrays.
We investigated the expression of 50 cytochrome P450 (CYP) genes in the postischemic reperfused rat liver using a DNA microarray. Thirteen CYPs showed absent expression in all experiments and 2 CYPs were induced by pentobarbital, which was used as an anesthetic. Eight of the remaining 35 CYPs showed significantly decreased expression following ischemia-reperfusion. Monitoring CYP expression may be a powerful approach for elucidation of pathways regulating drug metabolism that may be involved in postischemic reperfusion liver injury.
Takahashi
Yasuo
Y
Division of Genomic Epidemiology & Clinical Trials, Advanced Medical Research Center, Nihon University School of Medicine, Tokyo, Japan.
Nishida
Yayoi
Y
Ishii
Yukimoto
Y
Ishikawa
Koichi
K
Asai
Satoshi
S
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000098:blood supply*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010634:Phenobarbital / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013250:Steroid Hydroxylases / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D019362:Cytochrome P-450 CYP2B1; C025619:steroid 16-beta-hydroxylase; D010634:Phenobarbital