Cloning and expression of D-lactonohydrolase cDNA from Fusarium moniliforme in Saccharomyces cerevisiae.
A D-lactonohydrolase gene of about 1.1 kb was cloned from Fusarium moniliforme. The ORF sequence predicted a protein of 382 amino acids with a molecular mass of about 40 kDa. An expression plasmid carrying the gene under the control of the triose phosphate isomerase gene promotor was introduced into Saccharomyces cerevisiae, and the D-lactonohydrolase gene was successfully expressed in the recombinant strains.
Liu
Zhiqiang
Z
Laboratory of Biocatalysis, School of Biotechnology, Southern Yangtze University, Wuxi 214036, PR China.
Sun
Zhihao
Z
D000595:Amino Acid Sequence; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases; C501958:D-lactonohydrolase, Fusarium moniliforme