Effects of photodynamic therapy using a fractionated dosing of mono-L-aspartyl chlorin e6 in a murine tumor.
One of the 'second generation' photosensitizing agents is N-acetyl chlorin e6 (NPe6). This product has a strong absorbance band at 665 nm, permitting treatment at a greater depth of tumor than earlier agents based on porphyrin structures. We examined the effects of fractionated drug administration on photodynamic efficacy. Prior studies had shown that it is the level of NPe6 in the circulation that predicts for photodynamic efficacy, indicating vascular shut-down to be the predominant mode of tumor control. Although pharmacokinetic studies revealed that >99% of NPe6 was lost from the circulation, it appears that a fractionated dosage protocol can promote photodamage to neoplastic tissue in vivo. This study also indicated the potential utility of an implantable micro array for tumor irradiation.
Webber
John
J
Department of Surgery, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Leeson
Brendan
B
Fromm
David
D
Kessel
David
D
D000704:Analysis of Variance; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use*; D011166:Porphyrins / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays
D017319:Photosensitizing Agents; D011166:Porphyrins; C053434:Talaporfin