title: Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine alpha-proteobacterium isolated from Mediterranean sea water.
abstract: A Gram-negative, slightly halophilic, strictly aerobic, chemo-organotrophic bacterium was isolated from Mediterranean sea water near Valencia (Spain). 16S rRNA gene sequence comparisons showed that the isolate represented a separate branch within the alpha-3 subclass of the Proteobacteria, now included within the order 'Rhodobacterales'. Jannaschia helgolandensis was the closest relative, but their low sequence similarity and other features indicated that they were not related at the genus level. Isolate 5SM22T produced bacteriochlorophyll a and grew on solid media as regular salmon-pink colonies. Cells are motile rods, with polar flagella. The DNA G+C content is 59.1 mol%. Morphological, physiological and genotypic differences from related, thus far known genera support the description of Thalassobacter stenotrophicus gen. nov., sp. nov. with strain 5SM22T (=CECT 5294T=DSM 16310T) as the type strain.
authors.affiliation: Departamento de Microbiología y Ecología, Facultad de Biología, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, Campus de Burjassot, 46100 València, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Macián
authors.affiliation: Colección Española de Cultivos Tipo (CECT), Facultad de Biología, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Departamento de Microbiología y Ecología, Facultad de Biología, Universitat de València, Campus de Burjassot, 46100 València, Spain.
authors.forename: D R
authors.initials: DR
authors.lastname: Arahal
authors.affiliation: Colección Española de Cultivos Tipo (CECT), Facultad de Biología, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Departamento de Microbiología y Ecología, Facultad de Biología, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, Campus de Burjassot, 46100 València, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Garay
authors.affiliation: Lehrstuhl für Mikrobiologie, Technische Universität München, Am Hochanger 4, D-85350 Freising, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Ludwig
authors.affiliation: Lehrstuhl für Mikrobiologie, Technische Universität München, Am Hochanger 4, D-85350 Freising, Germany.
authors.forename: K H
authors.initials: KH
authors.lastname: Schleifer
authors.affiliation: Departamento de Microbiología y Ecología, Facultad de Biología, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, Campus de Burjassot, 46100 València, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Pujalte
mesh_terms: D025541:Bacteriochlorophyll A / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D025541:Bacteriochlorophyll A; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S