title: Identification of insertion sequence from a gamma-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26.
abstract: Tn5-derived mutants of the gamma-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, named ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan. kmiya@vos.nagaokaut.ac.jp
authors.forename: Keisuke
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Masao
authors.initials: M
authors.lastname: Fukuda
authors.forename: Masataka
authors.initials: M
authors.lastname: Tsuda
authors.forename: Masamichi
authors.initials: M
authors.lastname: Takagi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nagata
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D020578:Sphingomonas / Q000235:genetics* / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001556:Hexachlorocyclohexane