High-resolution blood-brain barrier permeability and blood volume imaging using quantitative synchrotron radiation computed tomography: study on an F98 rat brain glioma.
The authors previously provided evidence of synchrotron radiation computed tomography (SRCT) efficacy for quantitative in vivo brain perfusion measurements using monochromatic X-ray beams. However, this technique was limited for small-animal studies by partial volume effects. In this paper, high-resolution absolute cerebral blood volume and blood-brain barrier permeability coefficient measurements were obtained on a rat glioma model using SRCT and a CCD camera (47x47 microm2 pixel size). This is the first report of in vivo high-resolution brain vasculature parameter assessment. The work gives interesting perspectives to quantify brain hemodynamic changes accurately in healthy and pathological small animals.
Adam
Jean-François
JF
Unité INSERM 647 RSRM (Rayonnement Synchrotron et Recherche Médicale), ID17-ESRF, Grenoble, France.
Nemoz
Christian
C
Bravin
Alberto
A
Fiedler
Stefan
S
Bayat
Sam
S
Monfraix
Sylvie
S
Berruyer
Gilles
G
Charvet
Anne Marie
AM
Le Bas
Jean-François
JF
Elleaume
Hélène
H
Estève
François
F
D000818:Animals; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging*; D001921:Brain / Q000098:blood supply; D001932:Brain Neoplasms / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D002560:Cerebrovascular Circulation / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods; D005910:Glioma / Q000000981:diagnostic imaging*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D010539:Permeability; D051381:Rats; D017356:Synchrotrons; D014057:Tomography, X-Ray Computed / Q000379:methods*