Transcriptional regulation of the Schizosaccharomyces pombe gene encoding glutathione S-transferase I by a transcription factor Pap1.
In a previous study, a gst gene was isolated from the fission yeast Schizosaccharomyces pombe. This gene was dubbed gst I, and was characterized using the gstI-lacZ fusion plasmid pYSH2000. In this work, four additional fusion plasmids, pYSHSD1, pYSHSD2, pYSHSD3 and pYSHSD4, were constructed, in order to carry (respectively) 770, 551, 358 and 151 bp upstream regions from the translational initiation point. The sequence responsible for induction by aluminum, mercury and hydrogen peroxide was located in the range between -1,088 and -770 bp upstream of the S. pombe gst I gene. The same region was identified to contain the nucleotide sequence responsible for regulation by Pap1, and has one putative Pap1 binding site, TTACGTAT, located in the range between -954 approximately -947 bp upstream of the gst I gene. Negatively acting sequences are located between -1,088 and -151 bp. These findings imply that the Pap1 protein is involved in basal and inducible transcription of the gst I gene in the fission yeast S. pombe.
Kim
Hong-Gyum
HG
Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
Kim
Byung-Chul
BC
Kim
Kyunghoon
K
Park
Eun-Hee
EH
Lim
Chang-Jin
CJ
D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics*; D016350:Leucine Zippers / Q000235:genetics; D000073718:Pancreatitis-Associated Proteins; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors / Q000502:physiology*; D001616:beta-Galactosidase / Q000378:metabolism
D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D005982:Glutathione Transferase; D001616:beta-Galactosidase