Applications of array biosensor for detection of food allergens.
Although food is a necessity, compounds within food products can be dangerous and life-threatening for people with food allergies. These allergy-causing compounds, such as proteins from eggs and milk, must be identified on the labels of commercial products. Unintentional contamination of food with these compounds occurs as a result of storage, manufacturing procedures, or cleaning procedures. A sensitive, specific, and rapid method to identify foods containing allergens is required by the food industry. The array biosensor, a rapid detection system, may provide a solution to this need. The array biosensor performs fluorescent immunoassays on the surface of a planar waveguide by first running samples, then fluorescently labeled antibodies, over a surface patterned with capture antibodies. An optical image is captured by a charged-coupled device camera and converted into fluorescence values. Signal intensity and spot location provide information on the compound and its concentration. The array biosensor has been successfully demonstrated for toxin, bacteria, and virus detection at low levels in under 20 min in food, clinical samples, and environmental matrixes. An assay for detection of ovalbumin as an indicator of egg contamination has been developed with limits of detection of 25 pg/mL in buffer and 1.3 ng/mL (13 ng/g) in non-egg pasta extract (buffer:pasta 10:1, v/w).
Shriver-Lake
Lisa C
LC
Naval Research Laboratory, Washington, DC 20375, USA. lcs@cbmse.nrl.navy.mil
Taitt
Chris Rowe
CR
Ligler
Frances S
FS
D000485:Allergens / Q000032:analysis*; D000906:Antibodies / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis; D015374:Biosensing Techniques; D004527:Egg Proteins / Q000032:analysis / Q000276:immunology; D005506:Food Contamination; D005512:Food Hypersensitivity; D005516:Food Microbiology; D007091:Image Processing, Computer-Assisted; D007118:Immunoassay; D010047:Ovalbumin / Q000032:analysis / Q000276:immunology
D000485:Allergens; D000906:Antibodies; D001427:Bacterial Toxins; D004527:Egg Proteins; D010047:Ovalbumin