Mimicking carboxyterminal phosphorylation differentially effects subcellular distribution and cell-to-cell movement of Tobacco mosaic virus movement protein.
Phosphorylation of Tobacco mosaic virus movement protein (TMV-MP) at three carboxyterminal Ser/Thr sites negatively regulates TMV-MP gating function and viral spread in Nicotiana tabacum but not in Nicotiana benthamiana, indicating a host dependant inactivation strategy. Here, we examine the effect of mimicking carboxyterminal phosphorylation on cell-to-cell transport of TMV-MP protein itself in host plants Nicotiana clevelandii, N. benthamiana, Nicotiana glutinosa and N. tabacum. Since TMV-MP transport function was inactivated only in N. tabacum, this host was chosen to explore the contribution of individual carboxyterminal phosphorylation sites. Selective mimicking of phosphorylation at one site enhances TMV-MP cell-to-cell transport, whereas a negative effect requires mimicking of phosphorylation at two or three sites. Potentially, during viral infection in N. tabacum, MP phosphorylation may occur sequentially: first, MP phosphorylation at a single site might ensure effective viral movement; only thereafter, further phosphorylation events may lead to inactivation of TMV-MP transport function.
Trutnyeva
Kateryna
K
Max F. Perutz Laboratories, Department of Medical Biochemistry, University Departments at the Vienna Biocenter, Medical University of Vienna, Dr. Bohrgasse 9, A-1030 Vienna, Austria.
Bachmaier
Radostina
R
Waigmann
Elisabeth
E
D001483:Base Sequence; D002450:Cell Communication; D002473:Cell Wall / Q000821:virology; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D010766:Phosphorylation; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013347:Subcellular Fractions / Q000821:virology; D014026:Nicotiana / Q000254:growth & development / Q000502:physiology / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism*
D017931:DNA Primers; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins