Isolation of recombinant antibodies (scFvs) to grapevine virus B.
A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
Saldarelli
P
P
Istituto di Virologia Vegetale del CNR, Sezione di Bari, Italy. csvvps04@area.ba.cnr.it
Keller
H
H
Dell'Orco
M
M
Schots
A
A
Elicio
V
V
Minafra
A
A
D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D007128:Immunoglobulin Fragments / Q000276:immunology / Q000302:isolation & purification*; D019151:Peptide Library; D010942:Plant Viruses / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification*; D027843:Vitis / Q000821:virology*
D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; C055966:immunoglobulin Fv