title: Osteopontin regulates multiple functions contributing to human colon cancer development and progression.
abstract: Osteopontin (OPN) is a secreted phosphoglycoprotein known to interact with a number of integrin receptors. While increased OPN expression has been reported in a number of human cancers, and its cognate receptors (alphav-beta3, alphav-beta5, and alphav-beta1 integrins and CD44) have been identified, its role in colon cancer development and progression has not been extensively studied. We previously identified, using a combination of gene expression and tissue microarrays, that increased OPN expression is concordant with tumor stage. The current study examined the functional role of OPN in colon cancer progression and metastatic potential. The principal findings of this study were that both endogenous OPN expression (via stable transfection) as well as exogenous OPN (added to culture medium) enhanced the motility and invasive capacity of human colon cancer cells in vitro. OPN appeared to regulate motility though interaction with CD44. OPN expression also reduced intercellular (homotypic) adhesion, an important characteristic of metastatic cancer cells. Stable transfection of four poorly tumorigenic human colon cancer cell lines with OPN also resulted in enhanced tumorigenicity in vivo with increased proliferation and increased CD31 positive microvessel counts, concordant with the degree of OPN expression. Collectively, these results suggest that OPN may affect multiple functional components contributing to human colon cancer progression and solidifies its role in this process.
authors.affiliation: Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612-9497, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Irby
authors.forename: S M
authors.initials: SM
authors.lastname: McCarthy
authors.forename: T J
authors.initials: TJ
authors.lastname: Yeatman
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000098:blood supply / Q000188:drug therapy* / Q000556:secondary; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008833:Microcirculation; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009389:Neovascularization, Pathologic; D053495:Osteopontin; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012795:Sialoglycoproteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000637:transplantation
chemical_list: D018960:Hyaluronan Receptors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin