title: Rapid separation of microorganisms by quartz microchip capillary electrophoresis.
abstract: We developed and optimized a system coupling microchip capillary electrophoresis (MCE) and laser-induced fluorescence (LIF) detection for the analysis of microorganisms. The MCE-LIF system successfully separated pure cultures of lactic acid bacteria and Saccharomyces cerevisiae within 200 s. The results indicate that the MCE system can be conveniently used for the rapid and highly sensitive detection of microorganisms. Thus, MCE can provide a cheap and simple method for the on-line detection of microbial contamination.
authors.affiliation: Textile Industrial Institute of Ehime Prefecture, 2-5-48 Higashimura-minami Imabari, Ehime 799-1507, Japan. shintani@iri.pref.ehime.jp
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Shintani
authors.forename: Masaki
authors.initials: M
authors.lastname: Torimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sato
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tao
authors.forename: Takashi
authors.initials: T
authors.lastname: Manabe
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D046209:Electrophoresis, Microchip; D048668:Lactobacillus delbrueckii / Q000302:isolation & purification; D046208:Microchip Analytical Procedures; D011791:Quartz; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification; D048249:Streptococcus thermophilus / Q000302:isolation & purification
chemical_list: D011791:Quartz