DNA separation with low-viscosity sieving matrix on microfabricated polycarbonate microfluidic chips.
Microfluidic devices have been fabricated on polycarbonate (PC) substrates by use of a hot embossing method using a silicon master template. By adding auxiliary lines around the functional channel on the silicon master, its lifetime was significantly prolonged and the bonding strength of the PC cover plate to the microfluidic chip was greatly improved. More than 300 polycarbonate microfluidic chips have been replicated with the same silicon mold. CE separation of Phi X-174/HaeIII DNA restriction fragments, with high resolution efficiency and good reproducibility, was achieved on these devices using the low-viscosity sieving matrix HPMC-50. Temperature was found to have a significant effect on separation efficiency.
Ye
Mei-Ying
MY
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310027, P. R. China.
Yin
Xue-Feng
XF
Fang
Zhao-Lun
ZL
D010584:Bacteriophage phi X 174 / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification*; D006358:Hot Temperature; D044085:Microfluidics / Q000295:instrumentation*; D018613:Microscopy, Confocal; D008904:Miniaturization; D011076:Polycarboxylate Cement / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D014783:Viscosity
D004279:DNA, Viral; D011076:Polycarboxylate Cement; C005506:polycarbonate; D012825:Silicon