[Experimental evaluation of possible transmission of human alpha-2 interferon artificial gene to the other microorganism species].
Described within the case study are experimental evaluation data on the remote results of introduction of gene-engineered microorganisms (GEM) of the B-7092 B. subtilis strain into the environment with the follow-up ranging from 1 to 7 years. No bacteria of the above GEM were detected (among selected 19 bacterial cultures) in soil samples from an agricultural farm, where the VETOM 1.1 probiotic was used for the treatment and prevention of cattle; primary and precise identification (two-round PCR with specific primers) was applied. The introduction of the B-7092 B. subtilis strain into the environment did not result in its unlimited growth and proliferation in soil. No transfer of plasmid genes (genes of leukocytic human alpha-2 interferon) from the B-7092 B. subtilis strain GEM to microorganism species available in the environment (soil) was registered. No recombinant plasmid DNA from lysed GEM cells was detected in soil samples.
Stavskiĭ
E A
EA
Grishaeva
O N
ON
Leliak
A I
AI
Larina
N M
NM
Timukina
M A
MA
Mit'ko
L V
LV
Koval'
R S
RS
Serebrov
V V
VV
Renau
I V
IV
Kul'tenko
O V
OV
D000383:Agriculture; D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics* / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy; D022761:Gene Transfer, Horizontal; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000235:genetics*; D007962:Leukocytes / Q000378:metabolism; D010957:Plasmids / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D019936:Probiotics / Q000627:therapeutic use; D011994:Recombinant Proteins; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D012987:Soil