Chromatin remodeling by DNA bending, not twisting.
Single-stranded regions (gaps) in nucleosomal DNA interfere with action of the RSC chromatin-remodeling complex, monitored by exposure of restriction endonuclease cutting sites. Single-strand breaks (nicks) in the DNA, by contrast, have no effect. Gaps on one side of the cutting site are inhibitory, but gaps on the other side are not. A gap >100 bp from the cutting site is as effective as a gap <20 bp from the site. These findings suggest a remodeling process involving bending, but not twisting, of the DNA and further point to the propagation of a bent region (loop or bulge) from one end of the nucleosome to the other.
Lorch
Yahli
Y
Department of Structural Biology, School of Medicine, Stanford University, Stanford, CA 94305, USA.
Davis
Barbara
B
Kornberg
Roger D
RD
D020087:Alu Elements; D000818:Animals; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D007700:Kinetics; D009707:Nucleosomes / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014982:Xenopus laevis
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA