title: Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria.
abstract: Mitochondria perform a central function in the biogenesis of cellular iron-sulphur (Fe/S) proteins. It is unknown to date why this biosynthetic pathway is indispensable for life, the more so as no essential mitochondrial Fe/S proteins are known. Here, we show that the soluble ATP-binding cassette (ABC) protein Rli1p carries N-terminal Fe/S clusters that require the mitochondrial and cytosolic Fe/S protein biogenesis machineries for assembly. Mutations in critical cysteine residues of Rli1p abolish association with Fe/S clusters and lead to loss of cell viability. Hence, the essential character of Fe/S clusters in Rli1p explains the indispensable character of mitochondria in eukaryotes. We further report that Rli1p is associated with ribosomes and with Hcr1p, a protein involved in rRNA processing and translation initiation. Depletion of Rli1p causes a nuclear export defect of the small and large ribosomal subunits and subsequently a translational arrest. Thus, ribosome biogenesis and function are intimately linked to the crucial role of mitochondria in the maturation of the essential Fe/S protein Rli1p.
authors.affiliation: Institute of Biochemistry, University Medical School of Pecs, Hungary.
authors.forename: Gyula
authors.initials: G
authors.lastname: Kispal
authors.forename: Katalin
authors.initials: K
authors.lastname: Sipos
authors.forename: Heike
authors.initials: H
authors.lastname: Lange
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Fekete
authors.forename: Tibor
authors.initials: T
authors.lastname: Bedekovics
authors.forename: Tamás
authors.initials: T
authors.lastname: Janáky
authors.forename: Jochen
authors.initials: J
authors.lastname: Bassler
authors.forename: Daili J
authors.initials: DJ
authors.lastname: Aguilar Netz
authors.forename: Janneke
authors.initials: J
authors.lastname: Balk
authors.forename: Carmen
authors.initials: C
authors.lastname: Rotte
authors.forename: Roland
authors.initials: R
authors.lastname: Lill
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001693:Biological Transport, Active; D003600:Cytosol / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D018528:ATP-Binding Cassette Transporters; D004271:DNA, Fungal; C121939:HCR1 protein, S cerevisiae; D007506:Iron-Sulfur Proteins; D010448:Peptide Initiation Factors; C492990:RLI1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins