Solid-phase artificial chaperone-assisted refolding using insoluble beta-cyclodextrin-acrylamide copolymer beads.
Solid-phase refolding methods are advantageous since they facilitate both separation of solid additives from the refolded protein and recycling of the additives. Beta-cyclodextrin-acrylamide copolymer hydrogel beads were used as a matrix for detergents in solid-phase artificial chaperone-assisted refolding and improved the yield of lysozyme (up to 65%) and carbonic anhydrase B (up to 80%), compared with conventional solid host matrices.
Yamaguchi
Satoshi
S
Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Hong
Chai
C
Mannen
Teruhisa
T
Tsukiji
Sinya
S
Nagamune
Teruyuki
T
D020106:Acrylamide / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D024401:Carbonic Anhydrase I / Q000737:chemistry*; D004789:Enzyme Activation; D008863:Microspheres; D018832:Molecular Chaperones / Q000737:chemistry*; D009113:Muramidase / Q000032:analysis / Q000737:chemistry*; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry; D017510:Protein Folding; D020673:Protein Renaturation; D012995:Solubility; D047392:beta-Cyclodextrins / Q000737:chemistry*
D018832:Molecular Chaperones; D011108:Polymers; D047392:beta-Cyclodextrins; D020106:Acrylamide; D009113:Muramidase; D024401:Carbonic Anhydrase I; C031215:betadex