Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus.
In Brazil, the main etiologic agent of Leishmaniasis that frequently presents with mucosal involvement belongs to the Viannia subgenus. The therapeutic conduct in this disease depends on the parasitological diagnosis, and classical methods are restricted in identifying the agent. In this paper we describe a polymerase chain reaction (PCR), which uses primers designed from mini-exons repetitive sequences. The PCR amplifies a 177bp fragment that can distinguish (Viannia) from (Leishmania) subgenus. This test could be a useful diagnostic tool.
Paiva
Byanca Regina
BR
Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brazil.
Passos
Luciana Neves
LN
Falqueto
Aloisio
A
Malafronte
Rosely dos S
Rdos S
Andrade
Heitor Franco de
HF
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D005091:Exons / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000235:genetics*; D007896:Leishmaniasis / Q000175:diagnosis / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan