[Cytocompatibility of nanophase hydroxyapatite ceramics].
OBJECTIVE
To evaluate the cytocompatibility of nanophase hydroxyapatite ceramics in vitro.


METHODS
Hydroxyapatite (HA) was prepared via wet method. The grain size of the hydroxyapatite in the study was determined by scanning electron microscope and atomic force microscope with image analysis software. Primary osteoblast culture was established from rat calvaria. Cell adherence and proliferation on nanophase hydroxyapatite ceramics and conventional hydroxyapatite ceramics were examined at 1, 3, 5, 7 days. Morphology of the cells was observed by microscope.


RESULTS
The average grain size of the nanophase and conventional HA was 55 nm and 780 nm, respectively. Throughout 7 days period, osteoblast proliferation on the HA was similar to that on tissue culture borosilicate glass controls, osteoblasts could attach, spread and proliferate on HA. However, compared to conventional ceramics, osteoblast proliferation on nanophase HA was significantly better after 1, 3, 5 and 7 days.


CONCLUSIONS
Cytocompatibility of nanophase HA was significantly better than conventional ceramics.
Wen
Bo
B
Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Jilin University, Changchun 130041, China.
Chen
Zhi-qing
ZQ
Jiang
Yin-shan
YS
Yang
Zheng-wen
ZW
Xu
Yong-zhong
YZ
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics; D017886:Durapatite; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology*; D051381:Rats
D001672:Biocompatible Materials; D017886:Durapatite