title: Yuk-Hap-Tang induces apoptosis by intervening mn-SOD in human cervical carcinoma HeLa cells.
abstract: Yuk-Hap-Tang (YHT) induces cell death in human cervical carcinoma HeLa cells. Caspase-3, -6 and -9 were markedly activated in HeLa cells treated with YHT. The preferred substrate for caspase-3 cysteine protease, PARP, was cleaved to its 85-kDa cleavage product. YHT increased the amount of the anti-apoptotic protein, Bcl-2, and the pro-apoptotic protein, Bax. Although p53 has been reported to accumulate in cancer cells in response to anticancer agents, the p53 expression level was not changed in HeLa cells treated with YHT. Manganese (Mn)-TBAP, a mitochondria-specific SOD mimetic agent and NAC/GSH (N-acetyl cysteine/ reduced glutathione) reduced the YHT-induced cytotoxicity and decreased the number of the YHT-induced apoptotic cells. Furthermore, YHT reduced the expression of Mn-SOD protein and its activity in HeLa cells. The data demonstrate that YHT induces the apoptosis of human cervical carcinoma HeLa cells by intervening Mn-SOD.
authors.affiliation: Department of Pharmacology and the Institute of Cardiovascular Research, School of Medicine, Chonbuk National University, Jeonju, Chonbuk 565-701, South Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Chae
authors.forename: J M
authors.initials: JM
authors.lastname: Park
authors.forename: G Y
authors.initials: GY
authors.lastname: Lee
authors.forename: H R
authors.initials: HR
authors.lastname: Park
authors.forename: S W
authors.initials: SW
authors.lastname: Chae
authors.forename: G S
authors.initials: GS
authors.lastname: Jeong
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: S B
authors.initials: SB
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Yoo
authors.forename: H R
authors.initials: HR
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D016166:Free Radical Scavengers / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007723:Korea; D008518:Medicine, East Asian Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000235:genetics*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013482:Superoxide Dismutase; D020169:Caspases