The aadB gene cassette is associated with blaSHV genes in Klebsiella species producing extended-spectrum beta-lactamases.
Integrons were detected in 37 (72.5%) of 51 Klebsiella spp. producing extended-spectrum beta-lactamases by PCR with primers that targeted integrase genes and cassette regions. PCR and amplicon sequencing of the cassette regions revealed aadB and aadA2 gene cassettes that confer resistance to a range of aminoglycosides. aadB was associated with a class 1 integron on a 28-kb plasmid, pES1, that also contained bla(SHV-12) and IS26.
Jones
Louisa A
LA
School of Biotechnology and Biomolecular Sciences, Faculty of Science, University of New South Wales, Sydney 2052, Australia.
McIver
Christopher J
CJ
Kim
Mi-Jurng
MJ
Rawlinson
William D
WD
White
Peter A
PA
D000617:Aminoglycosides / Q000494:pharmacology; D022761:Gene Transfer, Horizontal / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D019426:Integrases / Q000235:genetics; D032023:Integrons / Q000235:genetics; D007709:Klebsiella / Q000235:genetics*; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
D000617:Aminoglycosides; D019426:Integrases; D001618:beta-Lactamases