title: Liquid chromatography determination of residue levels on apples treated with cetylpyridinium chloride.
abstract: Cetylpyridinium chloride (CPC) has been found to be effective in reducing microbial contamination in apples. A sensitive and specific HPLC method was developed to determine CPC residues in apples treated with CPC. This method involves ion exchange solid-phase extraction, and the use of stearylpyridinium chloride (SPC) as internal standard. Limit of quantitation, was 0.5 microg/ml of CPC for the apple ethanolic extracts. The observed residues in apple (2.35-4.35 microg/g of apple) were lower than those previously reported for chicken and beef. The method is specific, sensitive, reproducible and accurate.
authors.affiliation: Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 4301 West Markham, Slot 522-3, Little Rock, AR 72205, USA.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rodríguez-Morales
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Hamid
authors.initials: H
authors.lastname: Salari
authors.forename: Rafael
authors.initials: R
authors.lastname: Castillo
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Breen
authors.forename: Cesar M
authors.initials: CM
authors.lastname: Compadre
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D002138:Calibration; D002594:Cetylpyridinium / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D027845:Malus / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D000890:Anti-Infective Agents; D002594:Cetylpyridinium