Effects of D-myo-inositol 1-phosphate on the transfer function of phosphatidylinositol transfer protein alpha.
The lipid metabolite D-myo-inositol-1-phosphate is shown to increase the phospholipid transfer activity of phosphatidylinositol transfer protein alpha from liposomal and liver microsomal membranes. Dose-response curves indicated substantial enhancements of transfer in the low mM range that upon normalization were independent of membrane composition or the identity of the transferred phospholipid. The unnormalized effect is potentiated by anionic membrane surface charge and substantial membrane phosphatidylethanolamine content consistent with alterations of the protein's membrane binding affinity and alterations of surface electrostatic interactions as contributing factors.
Komatsu
Hiroaki
H
Department of Pathology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Westerman
Jan
J
Snoek
Gerry T
GT
Taraschi
Theodore F
TF
Janes
Nathan
N
D000818:Animals; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000008:administration & dosage / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000378:metabolism; D008564:Membrane Potentials; D008862:Microsomes, Liver / Q000378:metabolism; D049294:Phospholipid Transfer Proteins / Q000378:metabolism*
D004337:Drug Carriers; D007295:Inositol Phosphates; D008081:Liposomes; D008563:Membrane Lipids; D049294:Phospholipid Transfer Proteins; C002647:inositol 1-phosphate