A mutation in a cuticle collagen causes hypersensitivity to the endocrine disrupting chemical, bisphenol A, in Caenorhabditis elegans.
A novel mutant gene, bis-1 (bisphenol A sensitive) has been isolated in the nematode, Caenorhabditis elegans, that affects the response to endocrine disrupting chemicals (EDC). The bis-1(nx3) allele is hypersensitive to bisphenol A (BPA), is allelic to a collagen gene (col-121), and is expressed in hypodermal cells. Among the collagen mutants so far studied, bis-1(nx3), dpy-2(e8), dpy-7(e88) and dpy-10(e128) showed BPA sensitivity. The isolated mutant may work as a useful tool for the assay of EDC toxicity since the physiological effect of the collagen mutation (glycine substitution) indicates an increased sensitivity to BPA.
Watanabe
Masahito
M
Department of Cell Biology, Japanese Institute of Pearl Science, 4-28 Amanuma, Hiratsuka, Kanagawa 254-0031, Japan.
Mitani
Nanako
N
Ishii
Naoaki
N
Miki
Keizaburo
K
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001559:Benzhydryl Compounds; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D010636:Phenols / Q000494:pharmacology*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003094:Collagen; D016166:Free Radical Scavengers; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds