title: Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice.
abstract: Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S1161, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S1161 transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.
authors.affiliation: Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Wang
authors.forename: Hyung-Bae
authors.initials: HB
authors.lastname: Moon
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Seong-Lan
authors.initials: SL
authors.lastname: Yu
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Han
authors.forename: Wan
authors.initials: W
authors.lastname: Fang
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Lee
authors.forename: Kyung-Lib
authors.initials: KL
authors.lastname: Jang
authors.forename: Sang-Keun
authors.initials: SK
authors.lastname: Kim
authors.forename: Dae-Yeul
authors.initials: DY
authors.lastname: Yu
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006526:Hepatitis C / Q000473:pathology* / Q000821:virology; D006524:Hepatitis, Viral, Animal / Q000473:pathology* / Q000821:virology; D022781:Hepatocytes / Q000473:pathology / Q000821:virology; D008099:Liver / Q000473:pathology / Q000821:virology*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D019076:Transgenes; D014758:Viral Core Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus