title: [Cytocompatibility of nanophase hydroxyapatite ceramics].
abstract: OBJECTIVE
  To evaluate the cytocompatibility of nanophase hydroxyapatite ceramics in vitro.
  
  
  METHODS
  Hydroxyapatite (HA) was prepared via wet method. The grain size of the hydroxyapatite in the study was determined by scanning electron microscope and atomic force microscope with image analysis software. Primary osteoblast culture was established from rat calvaria. Cell adherence and proliferation on nanophase hydroxyapatite ceramics and conventional hydroxyapatite ceramics were examined at 1, 3, 5, 7 days. Morphology of the cells was observed by microscope.
  
  
  RESULTS
  The average grain size of the nanophase and conventional HA was 55 nm and 780 nm, respectively. Throughout 7 days period, osteoblast proliferation on the HA was similar to that on tissue culture borosilicate glass controls, osteoblasts could attach, spread and proliferate on HA. However, compared to conventional ceramics, osteoblast proliferation on nanophase HA was significantly better after 1, 3, 5 and 7 days.
  
  
  CONCLUSIONS
  Cytocompatibility of nanophase HA was significantly better than conventional ceramics.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Jilin University, Changchun 130041, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Yin-shan
authors.initials: YS
authors.lastname: Jiang
authors.forename: Zheng-wen
authors.initials: ZW
authors.lastname: Yang
authors.forename: Yong-zhong
authors.initials: YZ
authors.lastname: Xu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics; D017886:Durapatite; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology*; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite