title: The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
abstract: Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its approximately 20-megabase genome, which contains approximately 6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.
authors.affiliation: Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. bjloftus@tigr.org
authors.forename: Brendan J
authors.initials: BJ
authors.lastname: Loftus
authors.forename: Eula
authors.initials: E
authors.lastname: Fung
authors.forename: Paola
authors.initials: P
authors.lastname: Roncaglia
authors.forename: Don
authors.initials: D
authors.lastname: Rowley
authors.forename: Paolo
authors.initials: P
authors.lastname: Amedeo
authors.forename: Dan
authors.initials: D
authors.lastname: Bruno
authors.forename: Jessica
authors.initials: J
authors.lastname: Vamathevan
authors.forename: Molly
authors.initials: M
authors.lastname: Miranda
authors.forename: Iain J
authors.initials: IJ
authors.lastname: Anderson
authors.forename: James A
authors.initials: JA
authors.lastname: Fraser
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Allen
authors.forename: Ian E
authors.initials: IE
authors.lastname: Bosdet
authors.forename: Michael R
authors.initials: MR
authors.lastname: Brent
authors.forename: Readman
authors.initials: R
authors.lastname: Chiu
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Doering
authors.forename: Maureen J
authors.initials: MJ
authors.lastname: Donlin
authors.forename: Cletus A
authors.initials: CA
authors.lastname: D'Souza
authors.forename: Deborah S
authors.initials: DS
authors.lastname: Fox
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Grinberg
authors.forename: Jianmin
authors.initials: J
authors.lastname: Fu
authors.forename: Marilyn
authors.initials: M
authors.lastname: Fukushima
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Haas
authors.forename: James C
authors.initials: JC
authors.lastname: Huang
authors.forename: Guilhem
authors.initials: G
authors.lastname: Janbon
authors.forename: Steven J M
authors.initials: SJ
authors.lastname: Jones
authors.forename: Hean L
authors.initials: HL
authors.lastname: Koo
authors.forename: Martin I
authors.initials: MI
authors.lastname: Krzywinski
authors.forename: June K
authors.initials: JK
authors.lastname: Kwon-Chung
authors.forename: Klaus B
authors.initials: KB
authors.lastname: Lengeler
authors.forename: Rama
authors.initials: R
authors.lastname: Maiti
authors.forename: Marco A
authors.initials: MA
authors.lastname: Marra
authors.forename: Robert E
authors.initials: RE
authors.lastname: Marra
authors.forename: Carrie A
authors.initials: CA
authors.lastname: Mathewson
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Mitchell
authors.forename: Mihaela
authors.initials: M
authors.lastname: Pertea
authors.forename: Florenta R
authors.initials: FR
authors.lastname: Riggs
authors.forename: Steven L
authors.initials: SL
authors.lastname: Salzberg
authors.forename: Jacqueline E
authors.initials: JE
authors.lastname: Schein
authors.forename: Alla
authors.initials: A
authors.lastname: Shvartsbeyn
authors.forename: Heesun
authors.initials: H
authors.lastname: Shin
authors.forename: Martin
authors.initials: M
authors.lastname: Shumway
authors.forename: Charles A
authors.initials: CA
authors.lastname: Specht
authors.forename: Bernard B
authors.initials: BB
authors.lastname: Suh
authors.forename: Aaron
authors.initials: A
authors.lastname: Tenney
authors.forename: Terry R
authors.initials: TR
authors.lastname: Utterback
authors.forename: Brian L
authors.initials: BL
authors.lastname: Wickes
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Wortman
authors.forename: Natasja H
authors.initials: NH
authors.lastname: Wye
authors.forename: James W
authors.initials: JW
authors.lastname: Kronstad
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Lodge
authors.forename: Joseph
authors.initials: J
authors.lastname: Heitman
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
authors.forename: Claire M
authors.initials: CM
authors.lastname: Fraser
authors.forename: Richard W
authors.initials: RW
authors.lastname: Hyman
mesh_terms: D017398:Alternative Splicing; D002473:Cell Wall / Q000378:metabolism; D015825:Chromosomes, Fungal / Q000235:genetics; D019295:Computational Biology; D003455:Cryptococcus neoformans / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D004251:DNA Transposable Elements; D005656:Fungal Proteins / Q000378:metabolism; D015723:Gene Library; D005800:Genes, Fungal; D016681:Genome, Fungal; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011134:Polysaccharides / Q000378:metabolism; D016372:RNA, Antisense; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D005656:Fungal Proteins; D011134:Polysaccharides; D016372:RNA, Antisense; D037521:Virulence Factors