Multiple 5'-splice variants of the rat glutamate transporter-1.
In most brain areas, uptake of extracellular glutamate predominantly occurs through the glutamate transporter subtype, glutamate transporter-1 (GLT-1), which is enriched in astroglia. Here, we report the identification of five splice variants of the 5'-leader sequence of rat GLT-1 which contain varying numbers of upstream open reading frames and encode putative GLT-1 proteins with two distinct N-terminal modifications. We further demonstrate that the identified rat 5'-GLT-1 splice variants are expressed in a brain region-specific manner. Our findings point to potential influences of RNA splicing on glial glutamate transport in the intact and injured rat brain.
Rozyczka
Joanna
J
Institute of Anatomy, University of Leipzig, Medical Faculty, Leipzig, Germany.
Engele
Jürgen
J
D017398:Alternative Splicing; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D002490:Central Nervous System / Q000033:anatomy & histology / Q000378:metabolism; D027342:Excitatory Amino Acid Transporter 2 / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D014644:Genetic Variation / Q000502:physiology*; D022821:RNA Splice Sites / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D027342:Excitatory Amino Acid Transporter 2; D022821:RNA Splice Sites; D012333:RNA, Messenger