Coptidis rhizoma induces apoptosis in human colorectal cancer cells SNU-C4.
Coptidis rhizoma has been used as traditional herb medicine in gastrointestinal disorders in the Eastern Asia. We investigated whether the anticancer effects of the C. rhizoma induced apoptosis on human colorectal cancer cells SNU-C4. The cytotoxic effect of C. rhizoma was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. To determine apoptotic cell death, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) and caspase-3 enzyme assay were performed. In this study, C. rhizoma treatment (100 microg/ml) revealed typical morphological apoptotic features. Additionally, C. rhizoma treatment (100 microg/ml) increased levels of BAX and CASPASE-3, and decreased levels of BCL-2. Caspase-3 enzyme activity by treatment of C. rhizoma (100 microg/ml) also significantly increased compared to the control (p < 0.05). These data indicate that C. rhizoma caused cell death by apoptosis through caspase pathways on human colorectal cancer cells SNU-C4.
Kim
Youn Jung
YJ
Department of Dental Hygiene, Hanseo University, Seosan, Korea.
Kang
Soon Ah
SA
Hong
Mee Suk
MS
Park
Hae Jeong
HJ
Kim
Mi-Ja
MJ
Park
Hi-Jun
HJ
Kim
Hye Kyung
HK
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D015179:Colorectal Neoplasms; D000089042:Coptis chinensis; D017931:DNA Primers; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D007723:Korea; D016133:Polymerase Chain Reaction
D016588:Anticarcinogenic Agents; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; C107393:Coptidis rhizoma extract; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases