Electrochemical characteristics of a platinum electrode modified with a matrix of polyvinyl butyral and colloidal Ag containing immobilized horseradish peroxidase.
A new hydrogen peroxide biosensor was constructed, which consisted of a platinum electrode modified by a matrix of polyvinyl butyral (PVB) and nanometer-sized Ag colloid containing immobilized horseradish peroxidase (HRP), and using Co(bpy)3(3+) as mediator in the hydrogen peroxide solution. The electrochemical characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. The modified process was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The HRP immobilized on colloidal Ag was stable and retained its biological activity. The sensor displays excellent electrocatalytic response to the reduction of H2O2. Analytical parameters such as pH and temperature were also studied. Linear calibration for H2O2 was obtained in the range of 1x10(-5) to 1x10(-2) M under optimized conditions. The sensor was highly sensitive to H2O2, with a detection limit of 2x10(-6) M, and the sensor achieved 95% of steady-state current within 10 s. The sensor exhibited high sensitivity, selectivity and stability.
Yuan
Ruo
R
Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing, 400715, P. R. China. Yuanruo@swnu.edu.cn
Liu
Yan
Y
Li
Qun-Fang
QF
Chai
Ya-Qin
YQ
Mo
Chang-Li
CL
Zhong
Xia
X
Tang
Dian-Ping
DP
Dai
Jian-Yuan
JY
D002384:Catalysis; D003102:Colloids; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010984:Platinum; D012834:Silver / Q000032:analysis*
D003102:Colloids; D004800:Enzymes, Immobilized; D012834:Silver; D010984:Platinum; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase