Quartz-crystal microbalance-dissipation technique for the study of initial adsorption of fibronectin onto tresyl chloride-activated titanium.
The immobilization of cell-adhesive proteins onto titanium implants improves biological response at the implant-tissue interface. Previous studies demonstrated the easy and direct attachment of fibronectin onto titanium with the use of a 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) activation technique. The present study investigated the initial adsorption behavior of fibronectin on tresyl chloride-activated titanium by the quartz-crystal microbalance-dissipation (QCM-D) technique. The crystal resonant frequency and the dissipation shift of the oscillator were simultaneously measured by the injection of fibronectin/phosphate-buffered saline solution (pH = 7.4). The tresyl chloride-activated titanium surface showed a faster and greater decrease in frequency than that of untreated titanium, indicating that a greater amount of fibronectin was adsorbed in the former case during a 120-min adsorption. The dissipation-frequency plots revealed that, during the initial stage of adsorption, the bond between fibronectin and tresyl chloride-activated titanium is stronger than that between fibronectin and untreated titanium. The QCM-D technique can provide new insights into the adsorption mechanism of fibronectin.
Hayakawa
T
T
Department of Dental Materials, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-nishi, Matsudo, Chiba 271-8587, Japan. hayakawa@masact.nihon-u.ac.jp
Yoshinari
M
M
Nemoto
K
K
D000327:Adsorption; D001672:Biocompatible Materials; D015374:Biosensing Techniques; D020099:Coated Materials, Biocompatible; D003460:Crystallization; D005353:Fibronectins / Q000737:chemistry*; D008422:Materials Testing; D011791:Quartz; D013450:Sulfones / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D013450:Sulfones; D011791:Quartz; C032605:2,2,2-trifluoroethanesulfonyl chloride; D014025:Titanium