title: Localization of ubiquitin carboxyl-terminal hydrolase-L1 in cynomolgus monkey placentas.
abstract: Ubiquitin carboxyl-terminal hydrolase-L1 (UCH-L1) is a restrictedly expressed enzyme in neural and reproductive tissues, and it is considered to have a significant role in reproduction. In the present study, we investigated the localization of UCH-L1 in placenta of cynomolgus monkeys (Macaca fascicularis). UCH-L1 protein was detected in cytotrophoblasts of chorionic plate and villi, and decidual cells of decidua basalis in cynomolgus monkey placenta, and the amount of UCH-L1 protein in whole placenta increased as pregnancy progressed. These results supported that UCH-L1 is necessary for placental and fetal development in primate placenta. This is the first report to demonstrate the presence of UCH-L1 in primate placenta, and the cynomolgus monkey may be a useful model for the study of the functions of the ubiquitin-proteasome system in human pregnancy.
authors.affiliation: Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. aa37162@mail.ecc.u-tokyo.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Sekiguchi
authors.forename: A
authors.initials: A
authors.lastname: Takatori
authors.forename: T
authors.initials: T
authors.lastname: Negishi
authors.forename: J
authors.initials: J
authors.lastname: Kwon
authors.forename: T
authors.initials: T
authors.lastname: Kokubo
authors.forename: Y
authors.initials: Y
authors.lastname: Ishii
authors.forename: S
authors.initials: S
authors.lastname: Kyuwa
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005260:Female; D005865:Gestational Age; D007124:Immunoenzyme Techniques; D008252:Macaca fascicularis / Q000502:physiology*; D023421:Models, Animal; D010920:Placenta / Q000166:cytology / Q000201:enzymology*; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D044767:Ubiquitin-Protein Ligases; D043222:Ubiquitin Thiolesterase