title: Hydrogen peroxide produced by two amino acid oxidases mediates antibacterial actions.
abstract: The antibacterial actions of two amino acid oxidases, a D-amino acid oxidase from hog kidney and a L-amino acid oxidase from the venom of Agkistrodon halys, were investigated, demonstrating that both enzymes were able to inhibit the growth of both Gram-positive and Gram-negative bacteria, and that hydrogen peroxide, a product of their enzymatic reactions, was the antibacterial factor. However, hydrogen peroxide generated in the enzymatic reactions was not sufficient to explain the degree to which bacterial growth was inhibited. A fluorescence labeling assay showed that both of these two enzymes could bind to the surfaces of bacteria. To the best of our knowledge, this is the first report regarding the antibacterial activity of the D-amino acid oxidases.
authors.affiliation: Key Laboratory of Structural Biology, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, The People's Republic of China. zhanghm77@yahoo.com
authors.forename: Hongmin
authors.initials: H
authors.lastname: Zhang
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Yang
authors.forename: Mingxuan
authors.initials: M
authors.lastname: Sun
authors.forename: Maikun
authors.initials: M
authors.lastname: Teng
authors.forename: Liwen
authors.initials: L
authors.lastname: Niu
mesh_terms: D017836:Agkistrodon; D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002473:Cell Wall / Q000378:metabolism; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D015536:Down-Regulation; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D050830:L-Amino Acid Oxidase; D013552:Swine
chemical_list: D006861:Hydrogen Peroxide; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D003605:D-Amino-Acid Oxidase