Inhibitory avoidance task reveals differences in ectonucleotidase activities between male and female rats.
Studies demonstrated that endogenous levels of estrogen affect the long-term potentiation (LTP) and long-term depression (LTD). ATP and adenosine may play a role in the modulation of LTP. Our laboratory observed in previous studies that inhibitory avoidance task is associated with a decrease in hippocampal ectonucleotidase activities in adult male rats. To explore if ectonucleotidases are modulated in memory formation in female rats, as observed in males, we evaluated the effect of inhibitory avoidance training on synaptosomal NTP Dase and 5'-nucleotidase activities in rat hippocampus from both sexes. The results demonstrated a decrease in ATP, ADP and AMP hydrolysis (37%, 38% and 32%, respectively) immediately after training and a significant inhibition only in ATP hydrolysis (36%) 30 min post-training in male rats. There were no changes in ectonucleotidase activities from female rats. These findings provide support for the view that could exist biochemical differences in ectonucleotidase activities between males and females.
Rücker
Bárbara
B
Departamento de Bioquimica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Pereira
Grace S
GS
Fürstenau
Cristina R
CR
Izquierdo
Iván
I
Bonan
Carla D
CD
Sarkis
João J F
JJ
D015720:5'-Nucleotidase / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D004597:Electroshock; D005260:Female; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013574:Synaptosomes / Q000201:enzymology
D009419:Nerve Tissue Proteins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; C027201:ectoATPase