title: Changes in production and metabolism of brain natriuretic peptide in rats with myocardial necrosis.
abstract: In this study, we employed rat model of acute myocardial necrosis induced by isoproterenol (ISO) to study the possible roles of corin, the protease uniquely distributing in myocardium to convert pro-brain natriuretic peptide (proBNP) to BNP, and neutral endopeptidase (NEP), the major enzyme to degrade BNP, in changing the levels of BNP. In rats with isoproterenol alone, the myocardium necrosis occurred and the cardiac function was inhibited; the BNP contents in plasma and myocardium were upregulated, so did the myocardial corin mRNA level; the NEP activity in plasma and myocardium were downregulated. Omapatrilat (OMA) treatment relieved myocardial lesions and improved cardiac function. In the plasma and myocardium, omapatrilat treatment increased BNP contents, reduced NEP activity; in myocardium, mRNA level of proBNP and corin decreased, but NEP mRNA expression increased. Our study confirmed that omapatrilat treated myocardial necrosis effectively and suggested that increased BNP in rats with myocardial necrosis could depend on increased production and conversion as well as decreased degradation.
authors.affiliation: Institute of Cardiovascular Diseases, Peking University First Hospital, Beijing, No. 8, Xi Shiku street, Beijing 100034, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Cai
authors.forename: Chun-Shui
authors.initials: CS
authors.lastname: Pan
authors.forename: Yong-Fen
authors.initials: YF
authors.lastname: Qi
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Jiang
authors.forename: Bing
authors.initials: B
authors.lastname: Geng
authors.forename: Chao-Shu
authors.initials: CS
authors.lastname: Tang
mesh_terms: D000818:Animals; D007545:Isoproterenol / Q000633:toxicity; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D020097:Natriuretic Peptide, Brain / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D009336:Necrosis / Q000139:chemically induced / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013841:Thiazepines / Q000494:pharmacology
chemical_list: D011725:Pyridines; D013841:Thiazepines; D020097:Natriuretic Peptide, Brain; C106266:omapatrilat; D007545:Isoproterenol