AcrAB efflux pump plays a role in decreased susceptibility to tigecycline in Morganella morganii.
Transposon mutagenesis of a clinical isolate of Morganella morganii, G1492 (tigecycline MIC of 4 microg/ml), yielded two insertion knockout mutants for which tigecycline MICs were 0.03 microg/ml. Transposon insertions mapped to acrA, which is constitutively overexpressed in G1492, suggesting a role of the AcrAB efflux pump in decreased susceptibility to tigecycline in M. morganii.
Ruzin
Alexey
A
Wyeth Research, Department of Infectious Disease, 401 North Middletown Rd., Bld. 200, Rm. 3219, Pearl River, NY 10965, USA. ruzina@wyeth.com
Keeney
David
D
Bradford
Patricia A
PA
D004251:DNA Transposable Elements / Q000235:genetics; D005456:Fluorescent Dyes; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008969:Molecular Sequence Data; D020613:Morganella morganii / Q000187:drug effects* / Q000235:genetics*; D009154:Mutation / Q000502:physiology; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000078304:Tigecycline
D004251:DNA Transposable Elements; D005456:Fluorescent Dyes; D026901:Membrane Transport Proteins; D000078304:Tigecycline; D008911:Minocycline