title: Fluorescence in situ hybridization and chromosome studies after transfusion in newborns: is a waiting period necessary?
abstract: BACKGROUND
  Delaying chromosome studies after transfusion is common practice in many neonatal intensive care units (NICUs). Yet, no evidence exists to support this practice.
  
  
  OBJECTIVE
  To investigate the effects of filtration and irradiation on chromosome detection, and to evaluate donor chromosome interference after transfusion.
  
  
  METHODS
  Packed red blood cells (PRBCs) were evaluated by fluorescence in situ hybridization (FISH) and chromosome analyses. To evaluate donor leukocyte survival, blood was collected from female neonates who received male-donated PRBCs.
  
  
  RESULTS
  Irradiated, leukodepleted blood had no Y chromosome detection by FISH. Irradiated, microaggregate filtered blood had Y chromosome detection in all samples by FISH but no metaphase growth. No donor chromosomes were detected in neonates after transfusion.
  
  
  CONCLUSIONS
  Delaying chromosome or FISH analysis in transfused neonates who have received irradiated blood is unnecessary.
authors.affiliation: Advocate Lutheran General Children's Hospital, Department of Pediatrics, Park Ridge, Illinois, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Fynn
authors.forename: Violeta
authors.initials: V
authors.lastname: Komotos
authors.forename: Debra
authors.initials: D
authors.lastname: Rita
authors.forename: Raghbir
authors.initials: R
authors.lastname: Benawra
authors.forename: Lorene
authors.initials: L
authors.lastname: Schweig
authors.forename: Denise
authors.initials: D
authors.lastname: Angst
authors.forename: Bhagya
authors.initials: B
authors.lastname: Puppala
mesh_terms: D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006085:Graft Survival; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007231:Infant, Newborn; D007621:Karyotyping / Q000379:methods; D047589:Leukocyte Reduction Procedures / Q000379:methods; D007962:Leukocytes / Q000166:cytology; D008297:Male