Changes in sleep-wakefulness in the medial preoptic area lesioned rats: role of thermal preference.
Changes in sleep-wakefulness (S-W) were studied in adult male Wistar rats, along with body temperature (T(b)), locomotor activity (LMA) and thermal preference, after the lesion of the medial preoptic area (mPOA) with N-methyl-D-aspartic acid (NMDA). The sleep was decreased after the lesion of the mPOA, but there was recovery when the rats were given freedom to stay in an ambient temperature (T(amb)) which they preferred. When given a choice between three T(amb) (24, 27 and 30 degrees C), the rats preferred 27 degrees C before the mPOA lesion, and 24 degrees C during the initial days after the lesion. There was a shift in the thermal preference to 30 degrees C, on the fourth week after the lesion, which coincided with the considerable recovery of sleep. The preference for higher T(amb) probably helped to improve sleep, as T(amb) of 30 degrees C is known to promote sleep. When the lesioned rats were not given the freedom to select the T(amb), there was no recovery in sleep. The mPOA seems to be essential for increasing the durations of slow wave sleep (SWS) episodes, especially the light SWS (S1), as they remained shorter than the pre-lesion value, even when the rats were given freedom to stay in a preferred T(amb). The homeostatic recovery of sleep, especially the night time sleep, resulted in the disruption of circadian sleep rhythm. But, the LMA, T(b) and thermal preference maintained their diurnal variation. T(b) and LMA were elevated after the mPOA lesion and they remained so till the end of the study.
Ray
Baisali
B
Department of Physiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
Mallick
Hruda Nanda
HN
Kumar
Velayudhan Mohan
VM
D000704:Analysis of Variance; D000818:Animals; D001831:Body Temperature / Q000502:physiology*; D001833:Body Temperature Regulation; D002940:Circadian Rhythm; D004569:Electroencephalography / Q000379:methods; D004576:Electromyography / Q000379:methods; D004585:Electrooculography / Q000379:methods; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D011301:Preoptic Area / Q000293:injuries / Q000473:pathology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012890:Sleep / Q000502:physiology*; D013686:Telemetry / Q000379:methods; D013696:Temperature; D013997:Time Factors; D014851:Wakefulness / Q000502:physiology*