[Chemical analysis of bronchoalveolar lavage in diagnostics of pneumonia].
Samples of bronchoalveolar lavages, obtained from 182 patients hospitalised at Vseobecna fakultni nemocnice and Nemocnice Kralovske Vinohrady, Prague, were analysed using solid phase microextraction-gas chromatography. At least 9 %, using less strong criteria up to 20 % of samples, contained higher volatile fatty acids (VFA), indicating the presence of fermenting anaerobic bacteria, found neither by cultivation nor by microscopy. Further compounds appeared in most of chromatographic examinations, tentatively labelled as "alcohol" and "acetoin". The second one occurred almost solely in samples from patients receiving the intensive care including mechanical ventilation. If characterised more precisely, it may serve as a potential marker of patients' status.
Julák
Jaroslav
J
Institute of Imunology and Microbiology, First Faculty of Medicine, Charles University, Prague, Czech Republic. jaroslav.julak@lf1.cuni.cz
Stránská
Eva
E
Rosová
Vlasta
V
Dohnalová
Alena
A
D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002849:Chromatography, Gas; D005232:Fatty Acids, Volatile / Q000032:analysis; D006801:Humans; D018410:Pneumonia, Bacterial / Q000175:diagnosis*
D015415:Biomarkers; D005232:Fatty Acids, Volatile