Dose dependent accretion of docosahexaenoic acid (DHA) in cardiac membranes of rats fed egg yolk powder enriched in DHA.
We tested the hypothesis that enrichment of the diet with docosahexaenoic acid (DHA) enriched egg yolk powder could modify specifically the (n-3) fatty acids content of rat plasma, red blood cells and heart membranes. Dose-dependent effect of DHA was studied in rats supplemented during 4 weeks. Three groups of adult male rats, DHA10, DHA35 and DHA60 (n = 5 each), had their diet supplemented with 10 mg, 35 mg or 60 mg DHA/kg body weight/day, respectively. Fatty acid composition of membranes and plasma lipids were determined. A significant dose-dependent increase in DHA was observed in all three types of samples. Arachidonic acid (AA) levels did not change in heart and red blood cell membranes whereas it increased significantly in plasma with the DHA35 diet. These results contrast with that previously reported for fish oil supplementation where a decrease in AA levels was reported. Hence, DHA enriched egg yolk supplementation leads to a specific accretion of DHA without competition on AA status.
Gerbi
A
A
INSERM, U476, Faculté de Médecine Timone, Université Aix-Marseille, 27 Boulevard Jean Moulin, 13385 Marseille, France.
Bernard
M
M
Gleize
B
B
Coste
T C
TC
Maixent
J M
JM
Lan
C
C
Paganelli
F
F
Pieroni
G
G
D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D002462:Cell Membrane / Q000737:chemistry; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism*; D004530:Egg Yolk; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000097:blood; D006352:Heart Ventricles / Q000737:chemistry; D008297:Male; D008861:Microsomes / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000648:ultrastructure; D011208:Powders; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D015525:Fatty Acids, Omega-3; D011208:Powders; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid