Conjugated poly(D,L-lactide-co-glycolide) for the preparation of in vivo detectable nanoparticles.
Cellular localization of nanoparticles (Np) represents an important target in the understanding of their distribution after endovenous injection. The need of suitable devices and methodologies capable to detect Np in tissues or in cellular districts can be satisfied by Np which have to be easily recognizable by simple methods. Conjugations of poly(D,L-lactide-co-glycolide) with fluorescein and biotin allow fluorescent and immuno-histochemically active Np to be obtained. The fluorescein Np are detectable using fluorescent microscopy whereas biotin Np can be detected by optical microscopy after streptavidin-biotin-peroxidase complexation. In vivo experiments confirm the ability of these particles to be easily detected in the brain parenchyma or in the liver cell population according to the infusion pathway.
Tosi
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G
Dipartimento di Scienze Farmaceutiche, University of Modena and Reggio Emilia, Via Campi.183, 41100 Modena, Italy.
Rivasi
F
F
Gandolfi
F
F
Costantino
L
L
Vandelli
M A
MA
Forni
F
F
D000818:Animals; D001710:Biotin / Q000737:chemistry / Q000493:pharmacokinetics*; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D019793:Fluorescein / Q000737:chemistry / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes; D007262:Infusions, Intravenous; D019344:Lactic Acid / Q000737:chemistry*; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D014018:Tissue Distribution
D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D001710:Biotin; D019793:Fluorescein