[Experimental and clinical research of "Keyouling" on treatment of condyloma acuminata].
OBJECTIVE
To investigate the effects of traditional Chinese medicine "Keyouling" in treatment of condyloma acuminata (CA).


METHODS
The effects of "Keyouling" at different concentrations on growth and proliferation of rabbits' prepuce epithelial cells and CA warts tissue were assessed by means of cellular primary culture and subculture, and the effects of "Keyouling" at different concentrations on the expression level of HPV6.11 DNA and expression level of HPV6.11 DNA of different area of CA patient's penis were quantitatively detected by means of FQ-PCR.


RESULTS
"Keyouling" had no effect on growth and proliferation of rabbits' prepuce cuticle cells, but it had significant inhibition on CA warts tissue cells and expression of HPV6.11 DNA. Concentrations of "Keyouling" assumed negative correlated with cell proliferation rate of CA warts tissue and expression level of HPV6.11 DNA, and also had significant inhibition on expression of HPV6.11 DNA of negative cutaneous lesion area and cutaneous lesion area of CA patients.


CONCLUSIONS
"Keyouling" had significant anti HPV6.11 effect, could significantly restrain growth and proliferation of CA warts tissue cells, while it had no damaging effect on normal organic cuticle cells. It was suggested that "Keyouling" was safe in clinical use and had good therapeutic effect on CA and subclinical infection.
Jiang
Yi
Y
Department of Andrology, The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China.
Zhang
Shu-wu
SW
Wang
Jiu-yuan
JY
Wang
Cai-ming
CM
D000293:Adolescent; D000328:Adult; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003218:Condylomata Acuminata / Q000378:metabolism / Q000473:pathology*; D004279:DNA, Viral / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology; D052140:Human papillomavirus 11 / Q000235:genetics; D052163:Human papillomavirus 6 / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D010409:Penile Diseases / Q000378:metabolism / Q000473:pathology*; D010413:Penis / Q000378:metabolism / Q000473:pathology; D011817:Rabbits
D004279:DNA, Viral; D004365:Drugs, Chinese Herbal