Decreased sensory neuropeptide release in isolated bronchi of rats with cisplatin-induced neuropathy.
We studied if attenuated neurogenic bronchoconstriction was associated with a change in sensory neuropeptide release in preparations from rats with cisplatin-induced neuropathy. Electrical field stimulation (100 stimuli, 20 V, 0.1 ms, 20 Hz) induced an increase in the release of somatostatin, calcitonin gene-related peptide (CGRP) and substance P determined by radioimmunoassay from baseline 0.18+/-0.01, 0.17+/-0.01 and 0.86+/-0.02, to 0.59+/-0.02, 1.77+/-0.04 and 5.96 fmol/mg wet tissue weight, respectively, in organ fluid of tracheal tubes from rats. This was significantly attenuated to post-stimulation values of 0.36+/-0.02, 0.45+/-0.02, 4.68+/-0.24 fmol/mg wet tissue weight for somatostatin, CGRP, and substance P, respectively, with a significant decrease in field stimulation-induced contraction of bronchial preparations from animals 11 days after a 5-day treatment period with cisplatin (1.5 mg/kg i.p. once a day). The cisplatin-treated animals developed sensory neuropathy characterized by a 40% decrease in femoral nerve conduction velocity. The results show that a decrease in tracheo-bronchial sensory neuropeptide release associates with feeble bronchomotor responses in rats with cisplatin-induced sensory neuropathy.
Horvath
Peter
P
Department of Pharmacology and Pharmacotherapy, University of Debrecen, H-4032 Debrecen, Nagyerdei krt 98, Hungary.
Szilvassy
Judith
J
Nemeth
Jozsef
J
Peitl
Barna
B
Szilasi
Maria
M
Szilvassy
Zoltan
Z
D000818:Animals; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism*; D002945:Cisplatin / Q000633:toxicity*; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects / Q000502:physiology; D008297:Male; D009431:Neural Conduction / Q000187:drug effects / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism*; D011129:Polyradiculoneuropathy / Q000139:chemically induced* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009479:Neuropeptides; D002945:Cisplatin