title: Live birth of a normal healthy baby after a frozen embryo transfer with blastocysts that were frozen and thawed twice.
abstract: OBJECTIVE
  To report the birth of a normal healthy baby after a frozen ET (FET) with blastocysts that were frozen and thawed twice.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Private infertility practice.
  
  
  METHODS
  A 26-year-old female who presented with male factor infertility and polycystic ovarian disease.
  
  
  METHODS
  One cycle of IVF-ET and FET no. 1 followed by the refreezing of blastocysts and FET no. 2.
  
  
  METHODS
  Clinical pregnancy and live birth.
  
  
  RESULTS
  Thirty-six months after a successful IVF-ET cycle, FET no. 1 was performed with eight frozen blastocysts that were thawed. One blastocyst was transferred to the uterus and four were refrozen. The first FET resulted in a singleton pregnancy that ended in a spontaneous abortion after 7 weeks' gestation. Six months later, FET no. 2 was performed. All four refrozen blastocysts were thawed and transferred to the uterus. The second FET resulted in a singleton pregnancy and the birth of a normal healthy baby (male) weighing 3,005 g after 38 weeks' gestation.
  
  
  CONCLUSIONS
  Human blastocysts can be refrozen/thawed and produce a normal healthy baby after an FET. Further studies will be required to determine survival, implantation, and live birth rates with refrozen/thawed human blastocysts.
authors.affiliation: The Center for Reproductive Medicine, Lubbock, Texas 79410, USA. crmivflab@yahoo.com
authors.forename: L Keith
authors.initials: LK
authors.lastname: Smith
authors.forename: Ellen H
authors.initials: EH
authors.lastname: Roots
authors.forename: M Janelle Odom
authors.initials: MJ
authors.lastname: Dorsett
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000502:physiology*; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy