title: Agonists of toll-like receptors 2 and 4 activate airway smooth muscle via mononuclear leukocytes.
abstract: BACKGROUND
  Toll-like receptors 2 and 4 (TLR2, TLR4) enable cellular responses to bacterial lipoproteins, LPS, and endogenous mediators of cell damage. They have an established role in the activation of leukocytes, endothelial cells, and some smooth muscle cell types, but their roles in airway smooth muscle are uncertain.
  
  
  OBJECTIVE
  To determine the roles of TLRs in activation of airway smooth muscle.
  
  
  METHODS
  Airway smooth muscle cells were cultured with TLR agonists, in the presence or absence of mononuclear leukocytes.
  
  
  RESULTS
  We observed expression of TLR2 and TLR4 mRNAs, which could be upregulated by treatment with proinflammatory cytokines in primary human airway smooth muscle, but no important functional responses to agonists of these TLRs were seen. Coincubation of airway smooth muscle with peripheral blood mononuclear cells, at concentrations as low as 250 mononuclear cells/ml, resulted in a marked cooperative response to TLR stimuli, and synergistic production of cytokines, including chemokines (interleukin [IL-]-8) and IL-6. This cooperative response was greater when monocytes were enriched and was transferable using supernatants from LPS-stimulated peripheral blood mononuclear cells. Activation of cocultures required IL-1 generation from mononuclear cells, and was blocked by IL-1 receptor antagonist, though IL-1 generation alone was not sufficient to account for the magnitude of mononuclear cell-dependent coculture activation.
  
  
  CONCLUSIONS
  These data indicate that potent amplification of inflammation induced by TLR agonists, such as LPS, may be achieved by cooperativity between airway smooth muscle and leukocytes involved in immune surveillance or inflammation.
authors.affiliation: Academic Unit of Respiratory Medicine, Division of Genomic Medicine, University of Sheffield, M Floor, Royal Hallamshire Hospital, Sheffield, S10 2JF, UK.
authors.forename: Gavin E
authors.initials: GE
authors.lastname: Morris
authors.forename: Moira K B
authors.initials: MK
authors.lastname: Whyte
authors.forename: Gillian F
authors.initials: GF
authors.lastname: Martin
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Jose
authors.forename: Steven K
authors.initials: SK
authors.lastname: Dower
authors.forename: Ian
authors.initials: I
authors.lastname: Sabroe
mesh_terms: D016207:Cytokines / Q000235:genetics / Q000494:pharmacology; D004926:Escherichia coli; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000235:genetics / Q000494:pharmacology; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000276:immunology; D008211:Lymphocyte Cooperation / Q000187:drug effects* / Q000235:genetics; D009130:Muscle, Smooth / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D012132:Respiratory Muscles / Q000187:drug effects* / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D051195:Toll-Like Receptor 2 / Q000819:agonists* / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000819:agonists* / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4