title: Characterization of outer membrane protein fractions of Bdellovibrionales.
abstract: Bdellovibrio-and-like organisms (BALOs) are predatory bacteria that prey upon Gram-negative bacteria and are taxonomically subsumed in the order Bdellovibrionales. Despite their unique lifestyle, these bacteria show remarkable genotypic diversities. The outer membrane of the predators is likely to play an important role during the recognition and invasion stage, as well as in the intraperiplasmic growth phase. In this study, the outer membrane protein fractions of type strains of Bdellovibrio, Bacteriovorax and Peredibacter were investigated, revealing the presence of outer membrane proteins (Omps) similar to the major Omps of Bdellovibrio bacteriovorus. The primary structures of these Omps of Bdellovibrio sp. W, Bacteriovorax stolpii and Peredibacter starrii were elucidated by a combined mass spectrometric-reverse genetic approach. The similarity between the analyzed Omps of the investigated BALOs ranges from 32% to 89% showing conserved amino acid regions in their primary structure.
authors.affiliation: Department of Chemistry, Humboldt-Universitaet zu Berlin, 12489 Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Beck
authors.forename: Dominik
authors.initials: D
authors.lastname: Schwudke
authors.forename: Bernd
authors.initials: B
authors.lastname: Appel
authors.forename: Michael
authors.initials: M
authors.lastname: Linscheid
authors.forename: Eckhard
authors.initials: E
authors.lastname: Strauch
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001501:Bdellovibrio / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D001425:Bacterial Outer Membrane Proteins