title: Orally administered ethanol: transepidermal pathways and effects on the human skin barrier.
abstract: Ethanol intake is associated with a variety of skin diseases. The aim of the present study was (1) to identify the pathways of release of orally administered ethanol through the skin, and (2) to investigate the effects of a single oral dose of ethanol on the penetration of topically applied substances into the skin. Ethanol evaporation via the skin was measured using the new technique of ion mobility spectrometry (IMS). Transepidermal water loss (TEWL) and skin surface temperature were simultaneously measured before and after ethanol consumption. Measurements were performed on skin sites with different stratum corneum (SC) thickness, and density of follicles and sweat glands. These appendages were selectively sealed to investigate their participation in ethanol evaporation. The penetration of a topically applied UV filter substance was studied before and after ethanol consumption after removing the SC with adhesive tape. Ethanol evaporation was measured within 5 min of consumption, while the skin surface temperature remained nearly constant. The sealing of the appendages did not have a significant effect on ethanol evaporation. On the forehead, a higher TEWL value was measured than on the forearm. On both skin sites, an increase in TEWL was observed after ethanol ingestion. No influence of orally administered ethanol on the penetration of the topically applied UV filter substance was observed. The results indicate that ethanol evaporation occurs via the lipid layers without a significant effect on the penetration of the topically applied substance.
authors.affiliation: Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charité, 10098 Berlin, Germany. ute.jacobi@charite.de
authors.forename: Ute
authors.initials: U
authors.lastname: Jacobi
authors.forename: Jens
authors.initials: J
authors.lastname: Bartoll
authors.forename: Wolfram
authors.initials: W
authors.lastname: Sterry
authors.forename: Jürgen
authors.initials: J
authors.lastname: Lademann
mesh_terms: D000284:Administration, Oral; D000328:Adult; D002492:Central Nervous System Depressants / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004817:Epidermis / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D008297:Male; D012871:Skin Diseases / Q000378:metabolism; D012881:Skin Temperature; D013057:Spectrum Analysis / Q000379:methods; D014835:Volatilization
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol