Presence of CCK-A, B receptors and effect of gastrin and cholecystokinin on growth of pancreatobiliary cancer cell lines.
OBJECTIVE
To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines.


METHODS
Five pancreatic and 6 biliary cancer cell lines with 2 conrtol cells were used in this study. Cell proliferation study was done using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and direct cell count method. Reverse transcription-polymerase chain reaction (RT-PCR) and slot blot hybridization were performed to examine and quantify the expression of hormonal receptors in these cell lines.


RESULTS
SNU-308 showed a growth stimulating effect by gastrin-17, as did SNU-478 by both gastrin-17 and CCK-8. The trophic effect of these two hormones was completely blocked by specific antagonists (L-365, 260 for gastrin and L-364, 718 for CCK). Other cell lines did not respond to gastrin or CCK. In RT-PCR, the presence of CCK-A receptor and CCK-B/gastrin receptor mRNA was detected in all biliary and pancreatic cancer cell lines. In slot blot hybridization, compared to the cell lines which did not respond to hormones, those that responded to hormones showed high expression of receptor mRNA.


CONCLUSIONS
Gastrin and CCK exert a trophic action on some of the biliary tract cancers.
Jang
Jin-Young
JY
Department of Surgery, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul, 110-744, Korea.
Kim
Sun-Whe
SW
Ku
Ja-Lok
JL
Park
Yong-Hyun
YH
Park
Jae-Gahb
JG
D001661:Biliary Tract Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002766:Cholecystokinin / Q000378:metabolism / Q000494:pharmacology*; D005755:Gastrins / Q000378:metabolism / Q000494:pharmacology*; D006727:Hormone Antagonists / Q000494:pharmacology; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D043886:Receptor, Cholecystokinin A / Q000235:genetics* / Q000378:metabolism; D043887:Receptor, Cholecystokinin B / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles
D005755:Gastrins; D006727:Hormone Antagonists; D043886:Receptor, Cholecystokinin A; D043887:Receptor, Cholecystokinin B; D013778:Tetrazolium Salts; D013844:Thiazoles; C013550:gastrin 17; D002766:Cholecystokinin; C022616:thiazolyl blue