Extraction and proteomic analysis of proteins from normal and multiple sclerosis postmortem brain.
In this study, a reproducible fractionation procedure was developed to reduce levels of the abundant cytoskeletal proteins that are present in normal and pathological central nervous system (CNS) tissues. The fractionation and proteomic analysis techniques employed greatly facilitated comparison of the spectrum of proteins in normal postmortem brain with proteins in samples from patients with multiple sclerosis, an inflammatory demyelinating disease in which complex changes in protein expression occur as lesions develop. This approach may be of value for the proteomic identification and quantitation of proteins which undergo disease-related changes in CNS disorders, and also for protein expression studies on normal adult and developing CNS tissues.
Newcombe
Jia
J
NeuroResource, Department of Neuroinflammation, 1 Wakefield Street, Institute of Neurology, University College London, London WC1N 1PJ, England. j.newcombe@ion.ucl.ac.uk
Eriksson
Bodil
B
Ottervald
Jan
J
Yang
Yang
Y
Franzén
Bo
B
D000368:Aged; D001921:Brain / Q000473:pathology*; D001923:Brain Chemistry; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000302:isolation & purification; D011180:Postmortem Changes; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins