Measurement of 1J(Ni,Calpha(i)), 1J(Ni,C'i-1), 2J(Ni,Calpha(i-1)), 2J(H(N)i,C'i-1) and 2J(H(N)i,Calpha(i)) values in 13C/15N-labeled proteins.
Use of partial or selective (13)C/(15)N labeling of specific amino acid residues in a given protein to measure the values of (1)J((15)N(i),(13)C(alpha) (i)), (2)J((1)H(N),(13)C(alpha) (i)), (2)J((15)N(i),(13)C(alpha) (i-1)), (1)J((15)N(i),(13)C'(i-1)) and (2)J((1)H(N),(13)C'(i-1)) is described. This was achieved by recording a sensitivity-enhanced 2D [(15)N-(1)H] HSQC experiment, without mixing the spin states of C(alpha) and C' during the course of entire experiment.
Mukherjee
Sulakshana
S
Department of Chemical Sciences, Tata Institute of Fundamental Research, Mumbai 400 005, India.
Mustafi
Sourajit M
SM
Atreya
H S
HS
Chary
K V R
KV
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002118:Calcium / Q000737:chemistry; D002247:Carbon Isotopes / Q000737:chemistry; D004748:Entamoeba histolytica / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards*; D009587:Nitrogen Isotopes / Q000737:chemistry; D015800:Protozoan Proteins / Q000737:chemistry*; D012015:Reference Standards
D000596:Amino Acids; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D015800:Protozoan Proteins; D002118:Calcium