title: Multiple PolK (POLK) transcripts in mammalian testis.
abstract: The Y-family of DNA polymerases are capable of translesion synthesis both in vitro and in vivo. The mouse/human gene (Polkappa/POLK) that encodes the Y-family member Polk is highly expressed in mouse/human testis. Using RT-PCR to amplify the coding sequence of the PolK gene, 11 forms of mouse PolK and 5 forms of human POLK transcripts were identified from testis. This phenomenon is apparently specific to PolK since several other specialized DNA polymerases capable of TLS do not express functional alternative transcripts in the testis. The multiple mouse/human (PolK/POLK) transcripts may encode multiple Polkappa isoforms in testis.
authors.affiliation: Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9072, USA.
authors.forename: Caixia
authors.initials: C
authors.lastname: Guo
authors.forename: Tianshu
authors.initials: T
authors.lastname: Gao
authors.forename: Nils
authors.initials: N
authors.lastname: Confer
authors.forename: Susana
authors.initials: S
authors.lastname: Velasco-Miguel
authors.forename: Errol C
authors.initials: EC
authors.lastname: Friedberg
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*; D000099270:Y-Family DNA Polymerases
chemical_list: D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D012333:RNA, Messenger; C498613:Polk protein, mouse; D000099270:Y-Family DNA Polymerases