OsMADS22, an STMADS11-like MADS-box gene of rice, is expressed in non-vegetative tissues and its ectopic expression induces spikelet meristem indeterminacy.
We report the cDNA sequence and gene expression patterns of OsMADS22, a novel member of the STMADS11-like family of MADS-box genes, from rice. In contrast to previously reported STMADS11-like genes, whose expression is detected in vegetative tissues, OsMADS22 is mainly expressed during embryogenesis and flower development. In situ hybridization analysis revealed that OsMADS22 expression is localized in the L1 layer of embryos and in developing stamen primordia. Ectopic expression of OsMADS22 in transgenic rice plants resulted in aberrant floral morphogenesis, characterized by a disorganized palea, an elongated glume, and a two-floret spikelet. The results are discussed in terms of rice spikelet development and a novel non-vegetative role for a STMADS11-like gene.
Sentoku
Naoki
N
National Agricultural Research Center for Hokkaido Region, Toyohira, Sapporo, 062-8555, Japan. sennao@affrc.go.jp
Kato
Hideki
H
Kitano
Hidemi
H
Imai
Ryozo
R
D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D035264:Flowers / Q000378:metabolism*; D017403:In Situ Hybridization; D008163:Luminescent Measurements; D026161:MADS Domain Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D012639:Seeds / Q000378:metabolism; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018076:DNA, Complementary; D026161:MADS Domain Proteins; D010940:Plant Proteins