Use of magnetic beads to extract fungal DNA.
Authors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T. ajelloi, Epidermophyton floccosum, belonging to the dermatophytes group. Extraction products underwent polymerase chain reaction (PCR) fingerprinting with the appropriate primers to point out any disagreement in the genomic profiles. After having determined that the genomic profiles obtained from the DNA extracted from the same strain with the two methods correspond perfectly, the authors concluded that the extraction method with magnetic beads from fungal cells is simpler and quicker than with P/C extraction, greatly facilitating the obtainment of fungal DNA.
Faggi
E
E
Department of Public Health, Microbiology Unit, University of Florence, Florence, Italy.
Pini
G
G
Campisi
E
E
D003883:Arthrodermataceae / Q000145:classification / Q000235:genetics*; D002176:Candida albicans / Q000145:classification / Q000235:genetics*; D002725:Chloroform; D003455:Cryptococcus neoformans / Q000145:classification / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D008280:Magnetics; D016533:Mycological Typing Techniques; D019800:Phenol; D016133:Polymerase Chain Reaction / Q000379:methods; D011137:Polystyrenes
D004271:DNA, Fungal; D011137:Polystyrenes; D019800:Phenol; D002725:Chloroform