title: Influence of portal blood on the development of systemic inflammation associated with experimental acute pancreatitis.
abstract: BACKGROUND
  The liver is a source of systemic proinflammatory mediators in acute pancreatitis. We have investigated the effects of blood from the pancreas and intestine in liver activation and lung inflammation during early stages of experimental acute pancreatitis in a rat model.
  
  
  METHODS
  A portosystemic shunt and a mesosystemic shunt were created to prevent the passage of blood coming from the pancreas and the intestine, respectively, to the liver. Pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. After 3 hours, the inflammatory process in the lung and intestine, plasma levels of tumor necrosis factor (TNF)-alpha and their soluble receptor, and mRNA expression of inflammatory mediators in the lung were evaluated.
  
  
  RESULTS
  Portocaval shunting of blood prevented the inflammatory process in the lung, an increase in plasma TNF-alpha concentration, and the expression of TNF-alpha, interleukin (IL)-1beta, and heat-shock protein (HSP)-72 in the lung, but had no effect on plasma levels of soluble TNF-alpha receptor or on expression of inducible nitric oxide synthase (iNOS) and macrophage inflammatory protein (MIP)-2 in the lung. In contrast, mesocaval shunting of blood did not modify any of the parameters evaluated.
  
  
  CONCLUSIONS
  Pancreatic blood, but not intestinal blood, plays a key role in liver activation during experimental acute pancreatitis.
authors.affiliation: Department of Surgery, Hospital Clinic, University of Barcelona, c/Rosselló 161, 7o 08036 Barcelona, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Hoyos
authors.forename: Susana
authors.initials: S
authors.lastname: Granell
authors.forename: Nicolas
authors.initials: N
authors.lastname: Heredia
authors.forename: Oriol
authors.initials: O
authors.lastname: Bulbena
authors.forename: Daniel
authors.initials: D
authors.lastname: Closa
authors.forename: Laureano
authors.initials: L
authors.lastname: Fernández-Cruz
mesh_terms: D000208:Acute Disease; D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D007422:Intestines / Q000098:blood supply; D008099:Liver / Q000098:blood supply; D008168:Lung / Q000276:immunology; D008297:Male; D010179:Pancreas / Q000098:blood supply; D010195:Pancreatitis / Q000097:blood* / Q000209:etiology* / Q000235:genetics / Q000276:immunology; D011168:Portal System; D011170:Portasystemic Shunt, Surgical; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D012333:RNA, Messenger; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha