title: Optimum culture conditions for the production of N-substituted formamide deformylase by Arthrobacter pascens F164.
abstract: We investigated the optimum culture conditions for the production of a novel enzyme, N-substituted formamide deformylase, which acts mainly on N-benzylformamide, in Arthrobacter pascens F164. The highest enzyme activity was obtained when this strain F164 was cultivated in a synthetic medium with N-benzylformamide as sole nitrogen source. This deformylase was found to be an inducible enzyme depending on N-benzylformamide.
authors.affiliation: Institute of Applied Biochemistry, and Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fukatsu
authors.forename: Masahiko
authors.initials: M
authors.lastname: Goda
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Higashibata
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis*; D001173:Arthrobacter / Q000201:enzymology*; D003470:Culture Media / Q000737:chemistry; D004790:Enzyme Induction; D005559:Formamides / Q000378:metabolism
chemical_list: D003470:Culture Media; D005559:Formamides; D000581:Amidohydrolases; C493455:N-substituted formamide deformylase; C500252:N-benzylformamide