title: Recombinant Jembrana disease virus gag proteins identify several different antigenic domains but do not facilitate serological differentiation of JDV and nonpathogenic bovine lentiviruses.
abstract: In Indonesia, it is suspected that there are two bovine lentiviruses circulating in the cattle population: a pathogenic Jembrana disease virus (JDV), and a nonpathogenic bovine immunodeficiency-like virus (BIV). Both viruses cross-react antigenically and cannot be differentiated by current serological tests using JDV antigens. To identify possible type-specific epitopes, a series of recombinant protein constructs including the matrix, capsid and nucleocapsid proteins were produced from JDV gag and the expressed proteins were tested by Western blot using JDV and BIV hyperimmune sera. JDV matrix and truncated capsid proteins were recognised by both JDV and BIV hyperimmune sera indicating that there were multiple cross-reactive epitopes present in JDV gag. At least three epitopic regions were identified in these constructs, including the major homology region, by monoclonal antibody binding studies. JDV nucleocapsid recombinant protein was not recognised by either JDV or BIV hyperimmune sera and none of the recombinant gag proteins were able to differentiate between JDV positive sera from Jembrana disease endemic and Jembrana disease-free areas. Additionally, a 40 amino acid recombinant subunit protein encompassing the region recently found to contain an epitope unique to BIV [Zheng, L., Zhang, S., Wood, C., Kapil, S., Wilcox, G.E., Loughin, T.A., Minocha, H.C., 2001. Differentiation of two bovine lentiviruses by a monoclonal antibody on the basis of epitope specificity. Clin. Diagn. Lab. Immunol. 8, 283-287] was tested but was not recognised by either JDV positive sera from Jembrana disease-endemic or Jembrana disease-free areas.
authors.affiliation: Department of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia. mdesport@murdoch.edu.au
authors.forename: Moira
authors.initials: M
authors.lastname: Desport
authors.forename: Meredith E
authors.initials: ME
authors.lastname: Stewart
authors.forename: Carol A
authors.initials: CA
authors.lastname: Sheridan
authors.forename: William G F
authors.initials: WG
authors.lastname: Ditcham
authors.forename: Surachmi
authors.initials: S
authors.lastname: Setiyaningsih
authors.forename: W Masa
authors.initials: WM
authors.lastname: Tenaya
authors.forename: Nining
authors.initials: N
authors.lastname: Hartaningsih
authors.forename: Graham E
authors.initials: GE
authors.lastname: Wilcox
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D018604:Epitope Mapping; D015683:Gene Products, gag / Q000276:immunology*; D016087:Immunodeficiency Virus, Bovine / Q000276:immunology*; D017960:Lentiviruses, Bovine / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D011994:Recombinant Proteins