title: Frequency of factor V leiden mutation.
abstract: OBJECTIVE
  To determine the frequency of factor V leiden mutation.
  
  
  METHODS
  Observational study.
  
  
  METHODS
  One-year, January 2001 to December 2001 at the Armed Forces Institute of Pathology, Rawalpindi, Pakistan.
  
  
  METHODS
  Two hundred subjects each of apparently healthy and unrelated Punjabi and Pathan origins were included in the study. Peripheral blood samples were collected in EDTA and DNA extracted by phenol-chloroform extraction method. DNA analysis was done by PCR for restriction fragment length polymorphism. The product was digested overnight with Mn/1 and electrophoresed on acrylamide gel to detect 67 and 153 base pair fragments of factor V leiden against 37, 67 and 116 base pair fragments of normal factor V.
  
  
  RESULTS
  In the 400 subjects studied, only 5 cases of heterozygotes for factor V leiden were detected. The overall carrier rate was 1.3% (95% Cl 0.2-2.2%). The carrier rate in Punjabis and Pathans was 1% and 1.5% respectively.
  
  
  CONCLUSIONS
  This study confirms that the prevalence of factor V leiden is low in Asians and Africans as compared to the European population.
authors.affiliation: Department of Pathology, 136 Field Ambulance.
authors.lastname: Nasiruddin
authors.lastname: Zahur-ur-Rehman
authors.forename: Masood
authors.initials: M
authors.lastname: Anwar
authors.forename: Suhaib
authors.initials: S
authors.lastname: Ahmed
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ayyub
authors.forename: Waqar
authors.initials: W
authors.lastname: Ali
mesh_terms: D000328:Adult; D005165:Factor V / Q000235:genetics*; D005260:Female; D005820:Genetic Testing; D006801:Humans; D008297:Male; D010154:Pakistan / Q000453:epidemiology; D010865:Pilot Projects; D017354:Point Mutation; D015995:Prevalence; D019851:Thrombophilia / Q000453:epidemiology / Q000235:genetics
chemical_list: C095381:factor V Leiden; D005165:Factor V