Typing of Haemophilus paragallinarum strains by using enterobacterial repetitive intergenic consensus-based polymerase chain reaction.
The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) technique was used for fingerprinting of reference strains and Mexican isolates of Haemophilus paragallinarum. A total of nine ERIC patterns were given by the nine serovar reference strains of this bacteria. Two Modesto (C-2) reference strains from different sources showed the same ERIC pattern. Seventeen ERIC patterns were obtained among 29 Mexican isolates included in the study, belonging to serovars prevalent in Mexico (A-1, A-2, B-1, and C-2). Obtained results indicate that the ERIC-PCR technique could be used as a molecular laboratory tool for subtyping of H. paragallinarum.
Soriano
V E
VE
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, 50000, Mexico.
Téllez
G
G
Hargis
B M
BM
Newberry
L
L
Salgado-Miranda
C
C
Vázquez
J C
JC
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods / Q000662:veterinary; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry; D021901:DNA, Intergenic / Q000737:chemistry*; D014644:Genetic Variation; D044134:Haemophilus paragallinarum / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
D004269:DNA, Bacterial; D021901:DNA, Intergenic