DPPH free radical scavenger components from the fruits of Alpinia rafflesiana Wall. ex. Bak. (Zingiberaceae).
The methanol extract of the dried ripe fruits of Alpinia rafflesiana was investigated for its DPPH free radical scavenger constituents. 2',3',4',6'-Tetrahydroxychalcone (7), which has never been isolated from natural sources was found to be most active as a DPPH free radical scavenger with the IC50 value of 55 microM. Other known compounds isolated from this species include 5,6-dehydrokawain (1), flavokawin B (2). 1,7-diphenyl-5-hydroxy-6-hepten-3-one (3), (-)-pinocembrin (4), cardamonin (5) and (-)-pinostrobin (6). The DPPH free radical scavenger compounds were detected using TLC autographic analysis. The percentage inhibition of DPPH free radical scavenging activity was measured on isolates (5-7) using colorimetric analysis.
Mohamad
Habsah
H
Laboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Abas
Faridah
F
Permana
Dharma
D
Lajis
Nordin H
NH
Ali
Abdul Manaf
AM
Sukari
Mohd Aspollah
MA
Hin
Taufiq Y Y
TY
Kikuzaki
Hiroe
H
Nakatani
Nobuji
N
D032429:Alpinia / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006834:Hydrazines / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008296:Malaysia; D008958:Models, Molecular; D015394:Molecular Structure; D010851:Picrates; D012639:Seeds / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D001713:Biphenyl Compounds; D047188:Chalcones; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl