title: Chromatin remodeling by DNA bending, not twisting.
abstract: Single-stranded regions (gaps) in nucleosomal DNA interfere with action of the RSC chromatin-remodeling complex, monitored by exposure of restriction endonuclease cutting sites. Single-strand breaks (nicks) in the DNA, by contrast, have no effect. Gaps on one side of the cutting site are inhibitory, but gaps on the other side are not. A gap >100 bp from the cutting site is as effective as a gap <20 bp from the site. These findings suggest a remodeling process involving bending, but not twisting, of the DNA and further point to the propagation of a bent region (loop or bulge) from one end of the nucleosome to the other.
authors.affiliation: Department of Structural Biology, School of Medicine, Stanford University, Stanford, CA 94305, USA.
authors.forename: Yahli
authors.initials: Y
authors.lastname: Lorch
authors.forename: Barbara
authors.initials: B
authors.lastname: Davis
authors.forename: Roger D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D020087:Alu Elements; D000818:Animals; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D007700:Kinetics; D009707:Nucleosomes / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014982:Xenopus laevis
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D004247:DNA