A random peptide library fused to CCR5 for selection of mimetopes expressed on the mammalian cell surface via retroviral vectors.
A random peptide library was expressed on the surface of a mammalian cell by applying retroviral vectors. The random sequence was fused to the CCR5 chemokine receptor, which served as a scaffold to present the library at the cell surface. We used this library to isolate an epitope mimetope in a proof of principle system. This approach can become a tool for rapid creation of peptidic expression domains in a eukaryotic environment. Applications include the creation of decoys for receptors in cell-cell interactions, screening for molecules that drive ligand expression on target cells in two-cell interaction screens, among other utilities.
Wolkowicz
Roland
R
Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, School of Medicine, Stanford University, Stanford, California 94305-5175, USA.
Jager
Gina C
GC
Nolan
Garry P
GP
D001483:Base Sequence; D001671:Biochemistry / Q000379:methods*; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry; D005434:Flow Cytometry; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D019169:Jurkat Cells; D008024:Ligands; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D019713:Receptors, CCR5 / Q000737:chemistry*; D012190:Retroviridae / Q000235:genetics*
D018076:DNA, Complementary; D000939:Epitopes; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D019713:Receptors, CCR5; D004247:DNA