Can luminescent quantum dots be efficient energy acceptors with organic dye donors?
We assessed the ability of luminescent quantum dots (QDs) to function as energy acceptors in fluorescence resonance energy transfer (FRET) assays, with organic dyes serving as donors. Either AlexaFluor 488 or Cy3 dye was attached to maltose binding protein (MBP) and used with various QD acceptors. Steady-state and time-resolved fluorescence measurements showed no apparent FRET from dye to QD. We attribute these observations to the dominance of a fast radiative decay rate of the donor excitation relative to a slow FRET decay rate. This is due to the long exciton lifetime of the acceptor compared to that of the dye, combined with substantial QD direct excitation.
Clapp
Aaron R
AR
US Naval Research Laboratory, Optical Sciences Division, Code 5611, Washington, DC 20375, USA.
Medintz
Igor L
IL
Fisher
Brent R
BR
Anderson
George P
GP
Mattoussi
Hedi
H
D002232:Carbocyanines / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006834:Hydrazines / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D058619:Maltose-Binding Proteins; D045663:Quantum Dots
C417561:Alexa 488 hydrazide; D002232:Carbocyanines; D002352:Carrier Proteins; D006834:Hydrazines; D058619:Maltose-Binding Proteins; C089187:cyanine dye 3