Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography.
We studied the use of micellar electrokinetic capillary chromatography for separating eight penicillins. The method consists of (i) an electrophoretic separation based on micellar electrokinetic capillary chromatography, which uses sodium dodecyl sulfate (SDS) as surfactant; (ii) a sample stacking technique called reverse electrode polarity stacking mode (REPSM); and (iii) direct UV detection. The background electrolyte that gave complete separation contained 20 mM sodium borate buffer and 60 mM SDS. The sensitivity of the method was improved by an enrichment step that used on-column stacking. The limits of detection were at the microg.L(-1) level for the penicillins and did not detract from the peak resolution.
Puig
Patricia
P
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Borrull
Francesc
F
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Calull
Marta
M
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Aguilar
Carme
C
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D001881:Borates; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010406:Penicillins / Q000302:isolation & purification*; D010710:Phosphates; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate
D000900:Anti-Bacterial Agents; D001881:Borates; D002021:Buffers; D010406:Penicillins; D010710:Phosphates; D012967:Sodium Dodecyl Sulfate