title: Proteome analysis of skin distinguishes acute guttate from chronic plaque psoriasis.
abstract: Psoriasis is a disease with considerable heterogeneity in clinical presentation. This is the first study using two-dimensional gel electrophoresis to compare global protein expression patterns in lesional and non-lesional skin from subjects with acute guttate psoriasis associated with streptococcal throat infection and chronic plaque psoriasis. Samples from experimentally induced contact eczema and normal skin from healthy controls were also included. Proteins with statistically significant differences in expression were used in hierarchical cluster analyses resulting in separation of the different samples into groups. Chronic plaque and guttate psoriasis samples were distinctly separated, indicating that they represent discrete phenotypes at the protein expression level. Interestingly, there was a trend in which guttate psoriasis lesions clustered closer to eczema than to chronic plaque psoriasis lesions, indicating that the duration of the inflammatory reaction may affect clustering. Several of the differentially expressed proteins were identified by mass spectrometry.
authors.affiliation: Department of Dermatology, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Lina M
authors.initials: LM
authors.lastname: Carlén
authors.forename: Fabio
authors.initials: F
authors.lastname: Sánchez
authors.forename: Ann-Charlotte
authors.initials: AC
authors.lastname: Bergman
authors.forename: Susanne
authors.initials: S
authors.lastname: Becker
authors.forename: Daniel
authors.initials: D
authors.lastname: Hirschberg
authors.forename: Bo
authors.initials: B
authors.lastname: Franzén
authors.forename: Jonathan
authors.initials: J
authors.lastname: Coffey
authors.forename: Hans
authors.initials: H
authors.lastname: Jörnvall
authors.forename: Gert
authors.initials: G
authors.lastname: Auer
authors.forename: Ayodele A
authors.initials: AA
authors.lastname: Alaiya
authors.forename: Mona
authors.initials: M
authors.lastname: Ståhle
mesh_terms: D000208:Acute Disease; D002908:Chronic Disease; D016000:Cluster Analysis; D003937:Diagnosis, Differential; D004485:Eczema / Q000175:diagnosis / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D010641:Phenotype; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D011565:Psoriasis / Q000175:diagnosis* / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
chemical_list: D020543:Proteome