title: Optimisation of storage driven denitrification by using on-line specific oxygen uptake rate monitoring during SND in a SBR.
abstract: This study builds on previous experience of maximising the formation of COD as poly-hydroxybutyrate (PHB) and now describes a feedback technique of preserving the use of PHB for denitrification resulting in enhanced nitrogen removal rather than allowing its wasteful oxidation by oxygen. The feedback technique uses on-line SOUR monitoring for detecting the end-point of nitrification and controlling the aerobic phase length accordingly. The laboratory SBR was operated such that all organic substrate (acetate) was rapidly converted to PHB, which then served as the electron donor for nitrogen removal via simultaneous nitrification and denitrification (SND) during the aerobic phase (up to 70% SND). During SBR cycling with a fixed aeration length (240 minutes), PHB was unnecessarily oxidised after ammonium depletion, resulting in little denitrification and poor total nitrogen removal (69%). However, when the aerobic phase length was controlled via the SOUR, up to 1.8 CmM PHB (58 mg L(-1) COD) could be preserved, enabling improved total nitrogen removal (86%). The drop in the SOUR after ammonium depletion was a reproducible event that could be detected even when using raw wastewater and fresh activated sludge. The SOUR-control technique holds promise to build up PHB over a number of SBR cycles. While advanced oxygen-control is used for improved N-removal in several existing WWTPs, this study investigates the importance of oxygen control with relevance to PHB driven SND in sequencing batch reactors.
authors.affiliation: School of Biological Sciences and Biotechnology, Murdoch University, Murdoch, Western Australia 6150, Australia.
authors.forename: K A
authors.initials: KA
authors.lastname: Third
authors.forename: S
authors.initials: S
authors.lastname: Sepramaniam
authors.forename: Z
authors.initials: Z
authors.lastname: Tonkovic
authors.forename: M
authors.initials: M
authors.lastname: Newland
authors.forename: R
authors.initials: R
authors.lastname: Cord-Ruwisch
mesh_terms: D000332:Aerobiosis; D000641:Ammonia / Q000737:chemistry / Q000378:metabolism*; D019149:Bioreactors; D006885:Hydroxybutyrates / Q000378:metabolism*; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism; D009930:Organic Chemicals / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods; D000094122:Polyhydroxybutyrates
chemical_list: D000641:Ammonia; D006885:Hydroxybutyrates; D009573:Nitrites; D009584:Nitrogen; D009930:Organic Chemicals; D010100:Oxygen; D011091:Polyesters; D012722:Sewage; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates