Determination of the ionophoric coccidiostats narasin, monensin, lasalocid and salinomycin in eggs by liquid chromatography/tandem mass spectrometry.
A sensitive and selective liquid chromatographic tandem mass spectrometric method (LC/MS/MS) for the simultaneous detection of the ionophoric coccidiostats narasin, monensin, lasalocid and salinomycin in whole eggs has been developed. A very simple sample preparation consisting of an extraction with an organic solvent was carried out. Sample extracts were injected into the LC/MS/MS system on a C18 column and an isocratic elution was performed. Nigericin was used as internal standard. The precursor ions produced by electrospray positive ionisation were selected for collisional dissociation with argon into product ions. Validation of the methods was performed based on Commission Decision 2002/657/EC.1 CC(alpha) was found to be 1 microg/kg for all four compounds. Monitoring of Belgian egg samples in 2004 revealed that residues of salinomycin, lasalocid and monensin could be found.
Mortier
Leen
L
Ministry of the Flemish Community, Agricultural Research Centre Ghent (CLO), Department of Animal Product Quality and Transformation Technology (DVK), Brusselsesteenweg 370, 9090 Melle, Belgium. L.Mortier@clo.fgov.be
Daeseleire
Els
E
Van Peteghem
Carlos
C
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003049:Coccidiostats / Q000032:analysis*; D004531:Eggs; D005506:Food Contamination / Q000032:analysis*; D007476:Ionophores / Q000032:analysis*; D007832:Lasalocid / Q000032:analysis; D008985:Monensin / Q000032:analysis; D011714:Pyrans / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000095662:Polyether Polyketides
D003049:Coccidiostats; D007476:Ionophores; D007832:Lasalocid; D008985:Monensin; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides; C013612:narasin