title: Determination of oleanolic acid and ursolic acid in cornel by cyclodextrin-modified micellar electrokinetic chromatography.
abstract: A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method was established for the determination of oleanolic acid and ursolic acid in cornel. The two components were separated in the running buffer of 40 mmol/L sodium borate containing 5% methanol, 25 mmol/L SDS and 15 mmol/L hydroxypropyl-beta-cyclodextrin (HP-beta-CD). The applied voltage was 24 kV. The wavelength of detection was 200 nm. The temperature was kept at 25 C. Cinnamic acid was used as the internal standard. The analytical performance of the method was tested with respect to linearity, precision and recovery. The calibration curves were linear in the range of 10.15-243.6 microg/mL, r=0.9993 (oleanolic acid) and 10.07-241.7 microg/mL, r=0.9994 (ursolic acid); the intra-day precision (RSD) was less than 3.7% (oleanolic acid) and 4.1% (ursolic acid); the inter-day precision (RSD) was less than 4.2% (oleanolic acid) and 4.9% (ursolic acid). The limits of detection were 1.6 microg/mL for both components. The method proved to be sensitive, rapid, accurate and suitable for the determination of oleanolic acid and ursolic acid in cornel.
authors.affiliation: The Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Qi
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Lou
authors.forename: Changhai
authors.initials: C
authors.lastname: Liu
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Wu
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chai
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D029768:Cornus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D009828:Oleanolic Acid / Q000032:analysis*; D012680:Sensitivity and Specificity; D014315:Triterpenes / Q000032:analysis*; D047392:beta-Cyclodextrins / Q000737:chemistry*; D000097245:Ursolic Acid
chemical_list: D009828:Oleanolic Acid; D014315:Triterpenes; D047392:beta-Cyclodextrins; D000097245:Ursolic Acid