title: Involvement of the S100B in cAMP-induced cytoskeleton remodeling in astrocytes: a study using TRTK-12 in digitonin-permeabilized cells.
abstract: 1. Stellation of astrocytes in culture involves a complex rearrangement of microfilaments, intermediate filaments, and microtubules, which reflects in part the plasticity of these cells observed during development or after injury. 2. An astrocytic calcium-binding protein, S100B, has been implicated in the regulation of plasticity due to its ability to interact with cytoskeletal proteins. 3. We used digitonin-permeabilized astrocytes to introduce TRTK-12, a peptide that binds to the C-terminal of S100B and blocks its interaction with cytoskeletal proteins. 4. TRTK-12 was able to block cAMP-induced astrocyte stellation and this effect was dependent on the concentration of the peptide. These results support the idea that S100B has a modulatory role on astrocyte morphology.
authors.affiliation: Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Juliana K
authors.initials: JK
authors.lastname: Frizzo
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Tramontina
authors.forename: Francine
authors.initials: F
authors.lastname: Tramontina
authors.forename: Carmem
authors.initials: C
authors.lastname: Gottfried
authors.forename: Rodrigo B
authors.initials: RB
authors.lastname: Leal
authors.forename: Rosario
authors.initials: R
authors.lastname: Donato
authors.forename: Carlos-Alberto
authors.initials: CA
authors.lastname: Gonçalves
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D051347:CapZ Actin Capping Protein; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D004072:Digitonin / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009414:Nerve Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology; D010446:Peptide Fragments; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D051347:CapZ Actin Capping Protein; D009414:Nerve Growth Factors; D009842:Oligopeptides; D010446:Peptide Fragments; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat; C475482:TRTK-12 peptide; D000242:Cyclic AMP; D004072:Digitonin