title: Dynamic electric field assisted multi-dimensional liquid chromatography of biological samples.
abstract: Complex biological samples require very high resolution separation strategies. The platform introduced here capitalises on the hyphenation of liquid chromatographic (LC) and electric potential gradient electrochromatographic multi-dimensional separation genres. First-dimension selectivity is provided by simultaneous size exclusion (SEC) and strong cation exchange (SCX) chromatography modes, while the second dimension comprises reversed phase (RP) characteristics in a dynamic (time-variant) electric field. The time-variant potential gradient with reversal of polarity is applied across the second dimension monolithic capillary throughout the duration of the solvent strength gradient elution. Hence, the platform offers comprehensive on-line sample clean-up (matrix depletion, analyte enrichment), fractionation (first dimention LC), and separation (second dimension LC) with the prospect of altering selectivity via polarity reversal dynamic electric field tuning.
authors.affiliation: Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, Duesbergweg 10-14, D-55099 Mainz, Germany. tom.hennessy@ungerversum.de
authors.forename: T P
authors.initials: TP
authors.lastname: Hennessy
authors.forename: M
authors.initials: M
authors.lastname: Quaglia
authors.forename: O
authors.initials: O
authors.lastname: Kornysova
authors.forename: B A
authors.initials: BA
authors.lastname: Grimes
authors.forename: D
authors.initials: D
authors.lastname: Lubda
authors.forename: K K
authors.initials: KK
authors.lastname: Unger
mesh_terms: D001774:Blood Chemical Analysis; D002850:Chromatography, Gel / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012680:Sensitivity and Specificity