[Determination of alpha-cyperone in Rhizoma Cyperi oil by HPLC].
OBJECTIVE
To establish a method deteminating alpha-cyperone in Rhizome Cyperi oil by HPLC.


METHODS
The determination was carried out by Merck Lichrocart C18 column, with mobile phase consisted of methanol-water (68: 32), and detective wavelength 241 nm.


RESULTS
The calibration curves was linear in the range of 0. 081 - 0. 81 microg/ml (r = 0.9998). The average recovery of loading sample was 99.1%, RSD =2.2%.


CONCLUSIONS
The method is convenient for a good resolution.
Liang
Congqing
C
Guangdong College of Pharmacy, Guangzhou.
Luo
Yuansheng
Y
Zhong
Zhaojian
Z
Fu
Jun
J
Su
Chunyan
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D007659:Ketones / Q000032:analysis*; D019684:Magnoliopsida / Q000737:chemistry*; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027343:Rhizome / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D007659:Ketones; D009822:Oils, Volatile