Brevundimonas mediterranea sp. nov., a non-stalked species from the Mediterranean Sea.
Six strains of Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from the Mediterranean Sea. 16S rRNA gene sequence analysis indicated that the strains were affiliated within the alphaproteobacterial genus Brevundimonas, with Brevundimonas intermedia (99.4 %) and Brevundimonas vesicularis (99.2 %) as their closest relatives. This affiliation was supported by chemotaxonomic data (major polar lipids: phosphatidyl diacylglycerol, sulfoquinovosyl diacylglycerol and phosphatidyl glucopyranosyl diacylglycerol; major fatty acids: C(18 : 1), C(16 : 0), C(16 : 1), C(15 : 0), C(17 : 1)omega8c, 11-Me-C(18 : 1)omega5t). The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the strains from all recognized Brevundimonas species. The strains therefore represent a novel species, for which the name Brevundimonas mediterranea sp. nov. is proposed, with the type strain V4.BO.10T)(=LMG 21911T=CIP 107934T).
Fritz
Ingo
I
Max-Planck-Institute for Molecular Genetics, Department of Vertebrate Genomics, Ihnestraße 73, 14195 Berlin, Germany.
Strömpl
Carsten
C
GBF - National Research Center for Biotechnology, Division of Microbiology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Nikitin
Denis I
DI
Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Octyabrya 7, korp 2, Moscow 117811, Russia.
Lysenko
A M
AM
Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Octyabrya 7, korp 2, Moscow 117811, Russia.
Abraham
Wolf-Rainer
WR
GBF - National Research Center for Biotechnology, Division of Microbiology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
D042063:Caulobacteraceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S