Single nucleotide polymorphism for animal fibre identification.
Animal fibres are highly valuable industrial products often adulterated during marketing. Currently, there is no precise method available to identify and differentiate the fibres. In this study, a PCR-RFLP technique was exploited to differentiate cashmere and wool fibres derived from goat and sheep, respectively. The presence of DNA in animal hair shafts has enabled the isolation of DNA from scoured cashmere and wool fibres. The mitochondrial cytochrome b sequences of both species were amplified by PCR using primers designed from conserved regions. The polymorphism observed between the two species was detected by restricting the amplified product by endonucleases viz., BamH1 and Ssp1. The RFLP profile clearly distinguishes the cashmere and wool fibres and this technique can also be exploited to test adulteration in animal fibres qualitatively.
Subramanian
Selvi
S
Department of Biotechnology, PSG College of Technology, Coimbatore 641 004, India. selvi@scientist.com
Karthik
T
T
Vijayaraaghavan
N N
NN
D000818:Animals; D016172:DNA Fingerprinting / Q000379:methods*; D006041:Goats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012756:Sheep / Q000235:genetics*; D013784:Textiles / Q000032:analysis* / Q000145:classification*