Identification of 14-hydroxy-retro-retinol and 4-hydroxy-retinol as endogenous retinoids in rats throughout neonatal development.
14-Hydroxy-retro-retinol was previously described as an in vivo and in vitro metabolite of retinol. Furthermore, the retinoid 4-hydroxy-retinol was identified as an endogenous occurring retinoid in the amphibian organism and an in vitro metabolite of retinol. We describe in the present study that 14-hydroxy-retro-retinol and 4-hydroxy-retinol are present in normal neonatal rat serum as endogenous occurring retinoids in normal non-vitamin A supplemented mammals (rats). Both retinoids were detected in serum and liver of neonatal rats at days 3 and 11 after birth. The respective concentrations at day 11 after birth were 41.8 +/- 2.8 ng/ml (serum)/ 104 +/- 6 ng/g (liver) for 4-hydroxy-retinol and 23 +/- 4.6 ng/ml (serum)/ 285 +/- 5 ng/g (liver) for 14-hydroxy-retro-retinol. Both retinoids could not be detected in adult rat serum and liver. From our experiments important physiological functions of these retinoids during postnatal development could be postulated.
Rühl
Ralph
R
Institute of Nutritional Sciences, University of Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany. ralphruehl@web.de
Hamscher
Gerd
G
Garcia
Ada L
AL
Nau
Heinz
H
Schweigert
Florian J
FJ
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000378:metabolism*
C496908:4-hydroxy-retinol; D004224:Diterpenes; D014801:Vitamin A; C072014:14-hydroxy-4,14-retro-retinol