Effects of aluminium and lead on ATPase activity of knockout +/- mouse cerebral synaptosomes in vitro.
In this in vitro study, changes in the activity of the neural membrane integral protein, ATPase, were recorded after the exposure of isolated synaptosomes to different concentrations of aluminium and lead. Both total ATPase activity and Mg(2+)-ATPase activity were studied. A specific mouse strain, heterozygous for a glial cell line-derived neurotrophic factor (GDNF), and the corresponding wildtype mouse cerebral tissue, were used for the synaptosome isolations. The ATPase activities of the +/- mouse synaptosomes were compared with those of wild-type synaptosomes. The decrease in total ATPase activity was similar in both types of synaptosomes, but after exposure to aluminium, the decrease of Mg(2+)-ATPase activity in the GDNF+/- synaptosomes was smaller than that in the wild-type synaptosomes. After exposure to lead, the protective effect of GDNF was not so clear. The synaptosomal effects of lead were already found at concentrations lower than those where cell toxicity appeared in SH-SY5Y cell cultures. Thus, synaptosomal ATPase activity was considered to be a sensitive marker for the detection of lead-induced neurotoxicity.
Kohila
Tarja
T
Viikki Laboratory Animal Centre, University of Helsinki, Finland.
Tähti
Hanna
H
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000032:analysis; D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000648:ultrastructure*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006579:Heterozygote; D007854:Lead / Q000633:toxicity*; D051379:Mice; D018345:Mice, Knockout; D009414:Nerve Growth Factors / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D009447:Neuroblastoma; D013574:Synaptosomes / Q000201:enzymology*; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured
D015415:Biomarkers; C495040:Gdnf protein, mouse; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D007854:Lead; D000255:Adenosine Triphosphate; D000535:Aluminum; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase