Expression and function of Toll-like receptor 2 in canine blood phagocytes.
Toll-like receptors (TLRs) are a family of highly conserved pattern recognition receptors (PRR) of mammals that participate in the activation of innate immune responses against microbial infections. Among these receptors, TLR2 is essential for the recognition of conserved structural components of bacteria, protozoa and fungi. Until now, expression of TLR2 in dogs has not been investigated. In this work we describe a partial sequence of the gene coding for canine TLR2 and show that TLR2 mRNA is constitutively expressed in canine blood PMNs. We also show that stimulation of purified PMNs with lipoteichoic acid (LTA), a ligand of TLR2, leads to the release of proinflammatory chemokine IL-8. Furthermore, TLR2 protein is easily detectable by flow cytometry on the canine peripheral blood granulocyte and monocyte cell surface, and slightly on lymphocytes. These findings suggest that, also in dogs as in humans the initial antibacterial response of PMNs could be elicited through engagement of TLR2.
Bazzocchi
Chiara
C
Dipartimento di Patologia Animale Igiene e Sanità Pubblica Veterinaria, Sezione di Patologia Generale e Parassitologia, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
Mortarino
Michele
M
Comazzi
Stefano
S
Bandi
Claudio
C
Franceschi
Alberto
A
Genchi
Claudio
C
D000818:Animals; D001483:Base Sequence; D004285:Dogs / Q000097:blood / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D018375:Neutrophil Activation / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013682:Teichoic Acids / Q000276:immunology; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors
D016209:Interleukin-8; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D013682:Teichoic Acids; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; C009900:lipoteichoic acid