title: Experimental determination of viability loss of Penicillium bilaiae conidia during convective air-drying.
abstract: A study was conducted on the drying of Penicillium bilaiae, a fungal micro-organism used to promote soil-bound phosphorous uptake in several crop species, such as wheat, canola and pulse crops. A wet pellet formed from a mixture of the inoculant and a starch-based carrier was air-dried to the appropriate water activity to extend the shelf-life of the viable fungal conidia. Convective air-drying was examined as a low-energy alternative to the more expensive freeze-drying technology that is currently in use. Experiments were conducted to measure the loss of conidia viability during drying in a fixed-bed, thin-layer convective dryer. The dryer air inlet temperature and relative humidity were controlled in experiments to determine the effect of thermal and dessicative stresses on conidial viability. The measured survivor fraction was determined to be dependent on solids temperature, moisture content and drying rate. Thermal stresses became significant for process temperatures above 30 degrees C, while the survivor fraction fell sharply below a dry basis moisture ratio of 30%. Slower drying kinetics associated with high inlet air relative humidity were found to significantly improve the recovery of viable conidia. By minimising environmental stresses, survivor fractions of up to 75% could be achieved, but this result fell dramatically with the introduction of more severe conditions. A general linear statistical model is used to quantify experimental error and the significance level of each factor.
authors.affiliation: Department of Chemical Engineering, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Timothy
authors.initials: T
authors.lastname: Friesen
authors.forename: Gordon
authors.initials: G
authors.lastname: Hill
authors.forename: Todd
authors.initials: T
authors.lastname: Pugsley
authors.forename: Greg
authors.initials: G
authors.lastname: Holloway
authors.forename: Deanna
authors.initials: D
authors.lastname: Zimmerman
mesh_terms: D000388:Air; D003890:Desiccation; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D010758:Phosphorus / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014867:Water
chemical_list: D014867:Water; D010758:Phosphorus