New lignan metabolites in rat urine.
Ten potential lignan metabolites were quantified in rat urine extracts using liquid chromatography-tandem mass spectrometry. The rats were orally administered with the plant lignans 7-hydroxymatairesinol, matairesinol, lariciresinol or secoisolariciresinol, or with the mammalian lignan enterolactone. The samples were enzymatically hydrolysed and solid-phase extracted before analysis. Of the analysed compounds, only trace amounts of 7-oxoenterolactone could be detected in the urine extracts before administration, but after administration of any of the lignans, the excretion of 7-oxoenterolactone increased and monodemethylated matairesinol and 4,4'-dihydroxyenterolactone could be detected. In addition, other novel lignan metabolites were detected, i.e., 7-oxomatairesinol, alpha-conidendrin, and alpha- and beta-conidendric acid.
Smeds
Annika I
AI
Department of Organic Chemistry, Abo Akademi University, Biskopsgatan 8, FIN-20500 Turku/Abo, Finland. ansmeds@abo.fi
Saarinen
Niina M
NM
Eklund
Patrik C
PC
Sjöholm
Rainer E
RE
Mäkelä
Sari I
SI
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017705:Lignans / Q000378:metabolism* / Q000652:urine*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D017705:Lignans; C419306:hydroxymatairesinol