Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography.
A reversed phase high performance liquid chromatography method was developed for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors (NRTIs) lamivudine, didanosine, stavudine, zidovudine and abacavir in plasma. The method involved solid-phase extraction with Oasis MAX cartridges from plasma, followed by high performance liquid chromatography with a SymmetryShield RP 18 column and ultraviolet detection set at a wavelength of 260 nm. The assay was validated over the concentration range of 0.015-5 mg/l for all five NRTIs. The average accuracies for the assay were 92-102%, inter- and intra-day coefficients of variation (CV) were <2.5% and extraction recoveries were higher than 97%. This method proved to be simple, accurate and precise, and is currently in use in our laboratory for the quantitative analysis of NRTIs in plasma.
Verweij-van Wissen
C P W G M
CP
Department of Clinical Pharmacy, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. C.Verweij@akf.umcn.nl
Aarnoutse
R E
RE
Burger
D M
DM
D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016049:Didanosine / Q000097:blood; D015224:Dideoxynucleosides / Q000097:blood*; D004355:Drug Stability; D006678:HIV / Q000201:enzymology; D015658:HIV Infections / Q000188:drug therapy; D006801:Humans; D019259:Lamivudine / Q000097:blood; D011786:Quality Control; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood* / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D018119:Stavudine / Q000097:blood; D015215:Zidovudine / Q000097:blood; D000099227:Abacavir
D019380:Anti-HIV Agents; D016049:Didanosine; D015224:Dideoxynucleosides; D019259:Lamivudine; D018894:Reverse Transcriptase Inhibitors; D018119:Stavudine; D015215:Zidovudine; D000099227:Abacavir