title: Typing of Haemophilus paragallinarum strains by using enterobacterial repetitive intergenic consensus-based polymerase chain reaction.
abstract: The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) technique was used for fingerprinting of reference strains and Mexican isolates of Haemophilus paragallinarum. A total of nine ERIC patterns were given by the nine serovar reference strains of this bacteria. Two Modesto (C-2) reference strains from different sources showed the same ERIC pattern. Seventeen ERIC patterns were obtained among 29 Mexican isolates included in the study, belonging to serovars prevalent in Mexico (A-1, A-2, B-1, and C-2). Obtained results indicate that the ERIC-PCR technique could be used as a molecular laboratory tool for subtyping of H. paragallinarum.
authors.affiliation: Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, 50000, Mexico.
authors.forename: V E
authors.initials: VE
authors.lastname: Soriano
authors.forename: G
authors.initials: G
authors.lastname: Téllez
authors.forename: B M
authors.initials: BM
authors.lastname: Hargis
authors.forename: L
authors.initials: L
authors.lastname: Newberry
authors.forename: C
authors.initials: C
authors.lastname: Salgado-Miranda
authors.forename: J C
authors.initials: JC
authors.lastname: Vázquez
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods / Q000662:veterinary; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry; D021901:DNA, Intergenic / Q000737:chemistry*; D014644:Genetic Variation; D044134:Haemophilus paragallinarum / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic