Lentiviral transgenesis.
Transgenic animals are relevant for many fields of modern biomedicine and agriculture. However, the inefficiencies of the presently available techniques--DNA microinjection and retroviral gene transfer--have led to an explosion of costs for transgenics especially in farm animals. The recent success in transferring genes to early embryos of different species (mouse, rat, pig, cattle) by viral vectors derived from lentiviruses, has established lentiviral transgenesis as an exciting alternative to the classical method of DNA microinjection. In addition, lentiviral vectors can be used to transfer genes into embryonic stem cells. Due to its high efficacy and versatility, lentiviral transgenesis should have a big impact on transgenic research.
Pfeifer
Alexander
A
Department of Pharmacy, Institute for Pharmacology, Center for Drug Research, Ludwig-Maximilians University, Munich, Germany. alexander.pfeifer@cup.uni-muenchen.de
D000818:Animals; D000829:Animals, Domestic; D030801:Animals, Genetically Modified; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016086:Lentivirus