title: Dietary red palm oil improves reperfusion cardiac function in the isolated perfused rat heart of animals fed a high cholesterol diet.
abstract: It has been shown that dietary red palm oil (RPO) supplementation improved reperfusion function. However, no exact protective cellular mechanisms have been established. Our aim was to search for a possible cellular mechanism and a role for fatty acids. Rats were fed a standard rat chow, plus cholesterol and/or RPO-supplementation for 6 weeks. Functional recovery, myocardial phospholipid and cAMP/cGMP levels were determined in isolated rat hearts subjected to 25 min of normothermic total global ischaemia. Dietary RPO in the presence of cholesterol improved aortic output (AO) recovery (63.2+/-3.06%, P<0.05) vs. cholesterol only (36.5+/-6.2%). The improved functional recovery in hearts supplemented with RPO vs. control was preceded by an elevation in the cGMP levels early in ischaemia (RPO 132.9+/-36.3% vs. control 42.7+/-24.4%, P<0.05). Concurrently, cAMP levels decreased (RPO -8.3+/-6.9% vs. control 19.9+/-7.7%, P<0.05). Our data suggest that dietary RPO-supplementation improved reperfusion AO through mechanisms that may include activation of the NO-cGMP and inhibition of the cAMP pathway.
authors.affiliation: Faculty of Applied Sciences, Cape Technikon, Cape Town, South Africa.
authors.forename: A J
authors.initials: AJ
authors.lastname: Esterhuyse
authors.forename: E F
authors.initials: EF
authors.lastname: du Toit
authors.forename: A J S
authors.initials: AJ
authors.lastname: Benadè
authors.forename: J
authors.initials: J
authors.lastname: van Rooyen
mesh_terms: D000818:Animals; D002302:Cardiac Output; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D003326:Coronary Circulation; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D006321:Heart / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000178:diet therapy* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism; D000073878:Palm Oil; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage*; D051381:Rats; D020318:Rats, Long-Evans; D014280:Triglycerides / Q000097:blood
chemical_list: D002791:Cholesterol, Dietary; D010743:Phospholipids; D010938:Plant Oils; D014280:Triglycerides; D000073878:Palm Oil; D002784:Cholesterol; D000242:Cyclic AMP; D006152:Cyclic GMP