Biodiversity analysis of microbial community in the chem-bioflocculation treatment process.
Total DNA was directly extracted from environmental samples and amplified with polymerase chain reaction (PCR) technique. The PCR products were fingerprinted via denaturing gradient gel electrophoresis (DGGE). Significant differences were observed in the microbial community structures between traditional treatment process and chem-bioflocculation process. The microbial community structure shift at different sampling locations in chem-bioflocculation process and on two typical operational conditions was studied. 16S rDNA V3 regions of some dominant species were sequenced and the species were identified. The microbial communities were stable in both the chem-bioflocculation process and the activated sludge process under various experimental conditions presented in this work. The attached growth treatment process was less stable when operational conditions changed.
Xia
Siqing
S
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Engineering, Tongji University, 200092 Shanghai, China. siqingxia@mail.tongji.edu.cn
Wang
Feng
F
Fu
Yigang
Y
Yang
Dianhai
D
Ma
Xingmao
X
D001419:Bacteria; D044822:Biodiversity; D016172:DNA Fingerprinting; D004275:DNA, Ribosomal / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004783:Environmental Microbiology; D005427:Flocculation; D016133:Polymerase Chain Reaction; D011157:Population Dynamics; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014865:Waste Disposal, Fluid
D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S