Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro.
The effect of culture medium, washing, temperature, and sonication on the infectivity of Chlamydia pneumoniae for HL cells in experiments with heparin or heparinase treatment was studied. The presence of fetal calf serum (FCS) was necessary, and simple washing of elementary bodies increased their infectivity titer. Prolonged sonication was detrimental. Detailed description of the experimental conditions is necessary in studies of chlamydial attachment and infectivity.
Yan
Ying
Y
Department of Medical Microbiology, University of Oulu, P.O. Box 5000, 90014 Oulu, Finland. ying.yan@oulu.fi
Silvennoinen-Kassinen
Sylvi
S
Leinonen
Maija
M
Saikku
Pekka
P
D001431:Bacteriological Techniques / Q000379:methods*; D002690:Chlamydia Infections / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000254:growth & development / Q000472:pathogenicity*; D003470:Culture Media / Q000592:standards; D005260:Female; D006367:HeLa Cells; D006493:Heparin / Q000378:metabolism; D019763:Heparin Lyase / Q000378:metabolism; D006801:Humans; D044967:Serum / Q000378:metabolism
D003470:Culture Media; D006493:Heparin; D019763:Heparin Lyase