title: Construction of a [NiFe]-hydrogenase deletion mutant of Desulfovibrio vulgaris Hildenborough.
abstract: A mutant of Desulfovibrio vulgaris Hildenborough lacking a gene for [NiFe] hydrogenase was generated. Growth studies, performed for the mutant in comparison with the wild-type, showed no strong differences during the exponential growth phase. However, the mutant cells died more rapidly in the stationary growth phase.
authors.affiliation: Max-Planck Institut für Bioanorganische Chemie, Stiftstr. 34-36, 45470 Mülheim a.d. Ruhr, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Goenka
authors.forename: J K
authors.initials: JK
authors.lastname: Voordouw
authors.forename: W
authors.initials: W
authors.lastname: Lubitz
authors.forename: W
authors.initials: W
authors.lastname: Gärtner
authors.forename: G
authors.initials: G
authors.lastname: Voordouw
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D017931:DNA Primers; D016969:Desulfovibrio vulgaris / Q000201:enzymology* / Q000235:genetics; D006864:Hydrogenase / Q000235:genetics*
chemical_list: D017931:DNA Primers; C043296:nickel-iron hydrogenase; D006864:Hydrogenase