title: Method for localization of sialic acid on cell surface and cell interior in peripheral blood: a pilot study.
abstract: This article presents a method for identification and localization of cell surface and intracellular sialoglycoconjugates of peripheral blood cells. To reveal cell surface conjugates, a sample of peripheral blood was incubated with lectin after centrifugation and rinsing. For intracellular localization in leukocytes, RBCs were lysed and the membranes were permeabilized prior to cytochemical reaction. Fluorescein isothiocyanate conjugated lectins were used for visualization in fluorescence microscope. All lectins bound specifically to the surface of erythrocytes. Confocal microscopy showed surface and intracellular labeling of permeabilized leukocytes. A part of the signal in eosinophils originated from binding of anionic fluorophore to cationic granular proteins.
authors.affiliation: Department of Clinical Neuroscience, Karolinska Hospital, Stockholm, Sweden. maritta.ponnio@cns.ki.se
authors.forename: Maritta
authors.initials: M
authors.lastname: Pönniö
authors.forename: Jori
authors.initials: J
authors.lastname: Soukka
authors.forename: Pekka
authors.initials: P
authors.lastname: Sillanaukee
authors.forename: Johan
authors.initials: J
authors.lastname: Franck
authors.forename: Lahja
authors.initials: L
authors.lastname: Seveus
mesh_terms: D000328:Adult; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000378:metabolism; D007962:Leukocytes / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D010865:Pilot Projects
chemical_list: D037102:Lectins; D019158:N-Acetylneuraminic Acid