title: The fabrication of protein chip based on surface plasmon resonance for detection of pathogens.
abstract: Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
authors.forename: Woochang
authors.initials: W
authors.lastname: Lee
authors.forename: Bum Suk
authors.initials: BS
authors.lastname: Chun
authors.forename: Young Min
authors.initials: YM
authors.lastname: Bae
authors.forename: Won Hong
authors.initials: WH
authors.lastname: Lee
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D001419:Bacteria / Q000276:immunology* / Q000302:isolation & purification*; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008422:Materials Testing; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000276:immunology*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
chemical_list: D001672:Biocompatible Materials; C029152:G-substrate; D009419:Nerve Tissue Proteins