[Expression related to vascular endothelial growth factor C and induced nitride oxide synthesizase in lymph node micrometastasis of oral squamous cell carcinoma].
OBJECTIVE
To investigate the relationship of vascular endothelial growth factor C (VEGF-C) and induced nitride oxide synthesizase (iNOS) expression in lymph node micrometastasis of oral squamous cell carcinoma.


METHODS
Samples were obtained from 47 cases of oral squamous cell carcinoma and 15 cases with normal oral mucosa, VEGF-C and iNOS mRNA expression were detected by RT-PCR method. Lymph node micrometastasis of 10 normal lymph nodes and 355 lymph nodes from 47 cases of oral squamous cell carcinoma was detected with immunohistochemical reaction in cytokeratin antibody.


RESULTS
The percentages in tumors with higher expression were 57.4% for VEGF-C, 68.1% for iNOS (P < 0.05). They were significantly higher than that of normal groups. Significant positive relationship was found between VEGF-C and iNOS (P < 0.01). The positive rate of cytokeratin (CK) was 48.9%. Significant positive relationship was found between VEGF-C and CK, iNOS and CK (P < 0.01). The expression rates of CK in positive group of VEGF-C and iNOS were 63.0%, 65.6% respectively, and were significant higher than negative groups.


CONCLUSIONS
Expression of VEGF-C and iNOS in lymph node micrometastasis of oral squamous cell carcinoma is significant related.
Xie
Zhi-jian
ZJ
Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomaological Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.
Yang
Xiao-feng
XF
Wu
Qiu-liang
QL
Gu
Zhi-yuan
ZY
Zhang
Yin-kai
YK
D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008207:Lymphatic Metastasis; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D061206:Neoplasm Micrometastasis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*
D042582:Vascular Endothelial Growth Factor C; D007633:Keratins; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II