[A strategy for targeting gene therapy against cancer mediated by epidermal growth factor receptor].
OBJECTIVE
To establish a protocol for the targeting gene therapy against cancer with rich epidermal growth factor receptor (EGFR).


METHODS
A recombinant pcDNA3.1-PE III mut was constructed and combined with a non-viral vector, a fusion protein histone H1, epidermal growth factor C-loop previously expressed by us, to be a protein-DNA complex in vitro. Using the complex to treat BT-325 and Hela cancer cells with EGFR and JK cells without EGFR. The killing rates of the cells was calculated after 48 h of incubation at 37 degrees C.


RESULTS
To BT-325 and Hela cells, the killing rates were 46.03% and 48.12% respectively. To JK cells, the complex had no killing function.


CONCLUSIONS
The protocol for targeting gene therapy against cancer with EGFR has been established successfully.
Fang
Hua-Sheng
HS
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Hong
Mei
M
Zhang
Shu-Zheng
SZ
Lu
Sheng-Dong
SD
D036002:ADP Ribose Transferases / Q000235:genetics / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000235:genetics / Q000494:pharmacology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D002477:Cells; D004247:DNA / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D005098:Exotoxins / Q000235:genetics / Q000494:pharmacology*; D018390:Gene Targeting; D015316:Genetic Therapy; D005822:Genetic Vectors; D006657:Histones / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D014162:Transfection; D037521:Virulence Factors / Q000235:genetics / Q000494:pharmacology*; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D004247:DNA; D066246:ErbB Receptors; D005098:Exotoxins; D006657:Histones; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A