title: An on-line technique for monitoring propionic acid fermentation.
abstract: An on-line technique, based on measuring the increase in pressure due to CO(2) release in a closed air-tight reactor, was used to evaluate the fermentation of lactate by propionibacteria. The method was applied to batch cultures of Propionibacterium shermanii grown in yeast extract/sodium lactate medium containing lactate as a carbon source under micro-aerophilic conditions. Gas pressure evolution was compared both with substrate consumption and metabolites production and with acidification and growth. Linear relationships were found between gas pressure variation, lactate consumption and propionate and acetate production. The technique also enabled the evaluation of total CO(2) produced, by taking account of pressure, oxygen and pH measurements. These results tend to show that this simple and rapid method could be useful to monitor propionic acid bacteria growth.
authors.affiliation: Laboratoire Génie des Procédés Industriels UMR CNRS 6067, Département Génie Chimique, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, France.
authors.forename: Houda
authors.initials: H
authors.lastname: Benjelloun
authors.forename: Alice
authors.initials: A
authors.lastname: Rochex
authors.forename: Didier
authors.initials: D
authors.lastname: Lecouturier
authors.forename: Siham
authors.initials: S
authors.lastname: Dechemi
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Lebeault
mesh_terms: D000085:Acetates / Q000378:metabolism; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D019344:Lactic Acid / Q000378:metabolism; D008365:Manometry / Q000379:methods; D009862:Online Systems; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D011312:Pressure; D011422:Propionates / Q000378:metabolism*; D011424:Propionibacterium / Q000254:growth & development / Q000378:metabolism
chemical_list: D000085:Acetates; D011422:Propionates; D002245:Carbon Dioxide; D019344:Lactic Acid; C029658:propionic acid; D010100:Oxygen