A novel vesicle derived directly from endoplasmic reticulum is involved in the transport of vacuolar storage proteins in rice endosperm.
We found novel vesicles derived from rough endoplasmic reticulum (ER) in rice endosperm. The novel vesicles had characteristic structures different from that of the ER-derived protein body type I and the Golgi-derived dense vesicles. Immunocytochemical analysis revealed that the novel vesicles are derived directly from the aggregates of vacuolar storage proteins in the rough ER. In addition, BiP, an ER-resident molecular chaperone, was localized in the novel vesicles, but also in protein storage vacuoles (PSVs). These results suggest that the novel vesicles mediate transport of vacuolar storage proteins directly from the ER to PSVs in rice endosperm.
Takahashi
Hideyuki
H
Laboratory of Genetic Engineering, Graduate School of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan.
Saito
Yuhi
Y
Kitagawa
Tomoya
T
Morita
Shigeto
S
Masumura
Takehiro
T
Tanaka
Kunisuke
K
D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000378:metabolism; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism* / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D012275:Oryza / Q000378:metabolism* / Q000648:ultrastructure*; D010940:Plant Proteins / Q000378:metabolism*
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D010940:Plant Proteins; C077221:BIP protein, Arabidopsis