Large-scale and effective screening of Korean medicinal plants for inhibitory activity on matrix metalloproteinase-9.
Matrix metalloproteinase-9 (MMP-9) degrades type IV collagen constituting the major structural component of the basement membrane and extra cellular membrane. The enzymatic activity is found to be elevated in tumor tissues. With the aim of finding novel MMP-9 inhibitors from natural products, 87 extracts of oriental medicinal herbs, which are used as prescriptions for cancer treatment in traditional Korean medicine, were screened for their inhibitory activities towards MMP-9. It was found that most of the hexane and chloroform fractions as well as water extracts showed a weak inhibitory effect on MMP-9 activity at a concentration of 100mug/ml. However, a strong inhibition was found in the butanol fractions of Cinnamomum cassia PRESL, Magnolia obovata THUEB., Magnolia officinalis REHD. et WILS., Magnolia officinalis REHD. et WILS. var. biloba REHD. et WILS., and Euonymus alatus (THUNB.) SIEB. with inhibitory activity (>90%) at a concentration of 100 microg/ml.
Seo
Un-Kyo
UK
Department of Internal Medicine, Biochemistry and Pathology, College of Oriental Medicine, Dongguk University, Kyungju City, Kyungbuk 780-714, Republic of Korea.
Lee
Young-Ja
YJ
Kim
June-Ki
JK
Cha
Byung-Yoon
BY
Kim
Dong-Wook
DW
Nam
Kyung-Soo
KS
Kim
Cheorl-Ho
CH
D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D007723:Korea; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D008518:Medicine, East Asian Traditional; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018514:Plant Structures; D010946:Plants, Medicinal; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*
D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; D020780:Matrix Metalloproteinase 9