title: Detection of hypothetical proteins in 10 individual human tumor cell lines.
abstract: The search for new structures in tumors by genomics and proteomics methods is a major goal in tumor biology and may lead to the detection of markers or antigens for the generation of tumor vaccines. The aim of this study was to identify proteins that have been predicted so far based upon their nucleic acid sequence only or show poor identity to known proteins in tumor cell lines. Cell lines of neuroblastoma, colorectal, cervix carcinoma, adenocarcinoma of the ovary, lung and breast cancer, promyelocytic leukaemia, rhabdomyosarcoma, osteosarcoma and malignant melanoma were used. Cell lysates were run on 2D gel electrophoresis with subsequent in-gel digestion and MALDI-TOF-TOF analysis. A series of 10 hypothetical proteins (HPs) were observed and three of these proteins, hypothetical protein (Q9BTE6), CGI-83 protein (Q9Y392) and similar to CG11334 (Q9BV20), were so far described in tumors exclusively. The other seven proteins were already detected at the transcriptional level in normal and tumor cell lines or tissues. In conclusion, the three HPs observed in lung cancer and malignant melanoma may be candidates for development of tumor markers and generation of tumor vaccines.
authors.affiliation: Medical University of Vienna, Division of Basic Science, Department of Pediatrics, Währinger Gürtel 18, A-1090 Vienna, Austria.
authors.forename: Leila
authors.initials: L
authors.lastname: Afjehi-Sadat
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Maureen
authors.initials: M
authors.lastname: Felizardo
authors.forename: Kiseok
authors.initials: K
authors.lastname: Lee
authors.forename: Irene
authors.initials: I
authors.lastname: Slavc
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D045744:Cell Line, Tumor; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins