The use of an hydrogen peroxide multipurpose isolator for inhouse preparation of human embryo culture media: a unique successful Swiss randomized prospective study.
OBJECTIVE
To develop inhouse made (IHM) embryo culture medium with a Multipurpose Isolator and compare the embryo development in a prospective randomized study with commercial media.


METHODS
Fertilization by intracytoplasmic single sperm injection (ICSI) of Metaphase II oocytes obtained after 96 controlled ovarian hyperstimulation cycles in patients not older than 37 years. Transfer of zygotes to IHM or commercial Cook Sydney IVF Cleavage medium (SIC) immediately after pronucleus observation. Evaluation of embryo cleavage and score, pregnancy, and implantation rate.


RESULTS
From 100 zygotes cultured in SIC, 61% were at the 4 cell stage 45 h after ICSI compared to 77% (78/101) in the IHM, P < 0.05. The mean embryo score with IHM was 3.9 +/- 0.9 compared to 3.5 +/- 1.2 with SIC, P < 0.05. The clinical pregnancy rate per transfer was 38.9% (37/95), the implantation rate was 23% (46/200), and no differences were observed between the groups.
Van den Bergh
Marc J G
MJ
Fertility Laboratory, Frauenklinik, Kantonsspital Baden, Switzerland. marc.vandenbergh@ksb.ch
Siragusa
Antonino
A
Dubied
André
A
Vonbach
Priska
P
Deshe
Mary Fahy
MF
Flügel
Klara
K
Ruflin
Sandra
S
Stutz
Jacqueline
J
Teufelberger
Kirsten
K
Kratzer
Anette
A
Urech
Cornelia
C
Hohl
Michael
M
D000328:Adult; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D003470:Culture Media / Q000592:standards*; D004203:Disinfection / Q000379:methods; D010064:Embryo Implantation / Q000187:drug effects; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D006861:Hydrogen Peroxide / Q000627:therapeutic use*; D007185:Incubators; D008297:Male; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013557:Switzerland
D000891:Anti-Infective Agents, Local; D003470:Culture Media; D006861:Hydrogen Peroxide