Identification of epithelial auto-antigens associated with periodontal disease.
We previously reported evidence that patients with periodontitis have serum antibodies to oral Gram positive bacteria that are cross-reactive with epithelial antigens. In the present report cross-reactive epithelial antigens including CD24, lactate dehydrogenase A [LDM-A], antioxidant protein 2 [AOP 2] and nuclear factor of activated T cells 5 [NFAT 5], were identified by screening a cDNA expression library with pooled patient sera. Titres of antibodies to CD24 peptide correlated negatively with indices of periodontal disease severity. Strong expression of CD24 in the reactive periodontal epithelium and inflamed gingival attachment contrasted with low to undetectable expression in the external gingival epithelium. In periodontitis, a local action of these auto-reactive antibodies could modulate the regulatory potential associated with expression of CD24 in this epithelium.
Ye
P
P
Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, NSW, Australia.
Simonian
M
M
Nadkarni
M A
MA
Decarlo
A A
AA
Chapple
C C
CC
Hunter
N
N
D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D051927:CD24 Antigen; D003429:Cross Reactions; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000276:immunology; D000080864:Lactate Dehydrogenase 5; D008562:Membrane Glycoproteins / Q000276:immunology*; D009061:Mouth Mucosa / Q000276:immunology*; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology; D010544:Peroxidases / Q000276:immunology; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000276:immunology; D014157:Transcription Factors
D000942:Antigens, Bacterial; D015703:Antigens, CD; D001324:Autoantigens; D051927:CD24 Antigen; C497889:CD24 protein, human; D007527:Isoenzymes; D008562:Membrane Glycoproteins; C118125:NFAT5 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5; D010544:Peroxidases; C515977:PRDX6 protein, human; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins