Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites.
Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and M(r) between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin alpha- and beta-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons.
Lee
Eung-goo
EG
Department of Molecular Parasitology, School of Medicine, Sungkyunkwan University, Suwon, Republic of Korea.
Kim
Jae-hoon
JH
Shin
Yong-seung
YS
Shin
Gee-wook
GW
Kim
Young-rim
YR
Palaksha
K J
KJ
Kim
Dae-yong
DY
Yamane
Itsuro
I
Kim
Yong-hwan
YH
Kim
Gon-sup
GS
Suh
Myung-deuk
MD
Jung
Tae-sung
TS
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D013058:Mass Spectrometry; D018688:Neospora / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000254:growth & development; D020543:Proteome / Q000032:analysis; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics; D014122:Toxoplasma / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000254:growth & development
D000953:Antigens, Protozoan; D020543:Proteome; D015800:Protozoan Proteins