Cre-mediated recombination in cell lineages that express the progesterone receptor.
Using gene-targeting methods, a progesterone receptor Cre knockin (PR-Cre) mouse was generated in which Cre recombinase was inserted into exon 1 of the PR gene. The insertion positions the Cre gene downstream (and under the specific control) of the endogenous PR promoter. As for heterozygotes for the progesterone receptor knockout (PRKO) mutation, mice heterozygous for the Cre knockin insertion are phenotypically indistinguishable from wildtype. Crossing the PR-Cre with the ROSA26R reporter revealed that Cre excision activity is restricted to cells that express PR in progesterone-responsive tissues such as the uterus, ovary, oviduct, pituitary gland, and mammary gland. Initial characterization of the PR-Cre mouse underscores the utility of this model to precisely ablate floxed target genes specifically in cell lineages that express the PR. In the wider context of female reproductive tissue ontology, this model will be indispensable in tracing the developmental fate of cell lineages that descend from PR positive progenitors.
Soyal
Selma M
SM
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Mukherjee
Atish
A
Lee
Kevin Y-S
KY
Li
Jie
J
Li
Huaiguang
H
DeMayo
Francesco J
FJ
Lydon
John P
JP
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005821:Genetic Techniques; D006579:Heterozygote; D019426:Integrases / Q000235:genetics*; D007763:Lac Operon; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010053:Ovary / Q000378:metabolism; D010057:Oviducts / Q000378:metabolism; D010641:Phenotype; D010902:Pituitary Gland / Q000378:metabolism; D011980:Receptors, Progesterone / Q000172:deficiency / Q000235:genetics*; D011995:Recombination, Genetic; D014599:Uterus / Q000378:metabolism
D006063:Chorionic Gonadotropin; D011980:Receptors, Progesterone; C045073:Cre recombinase; D019426:Integrases