title: ACE inhibitor and angiotensin II type 1 receptor blocker differently regulate ventricular fibrosis in hypertensive diastolic heart failure.
abstract: BACKGROUND
  Promoted myocardial stiffening has a crucial role in the transition to overt diastolic heart failure (DHF) in hypertensive hearts and is attributed to progressive ventricular fibrosis. Previous studies revealed the effects of an angiotensin II type 1 receptor blocker (ARB) and an angiotensin-converting enzyme inhibitor (ACEI) on the synthesis and degradation of collagens in the other phenotype of heart failure, systolic heart failure, which has a different pathophysiology; however, little is known about their effects in DHF.
  
  
  OBJECTIVE
  To investigate effects of an ACEI and an ARB on the regulatory system of ventricular fibrosis in hypertensive DHF.
  
  
  METHODS
  Dahl salt-sensitive rats fed a diet containing 8% NaCl from age 7 weeks (DHF model) were divided into three groups: six untreated rats, six rats treated with a subdepressor dose of an ARB, candesartan cilexetil (1 mg/kg per day), from age 8 weeks, and six rats treated with a subdepress or dose of an ACEI, temocapril hydrochloride (0.2 mg/kg per day), from age 8 weeks. Six Dahl salt-sensitive rats fed on normal chow served as controls. Data were collected when animals were aged 20 weeks.
  
  
  RESULTS
  The administration of an ARB or an ACEI inhibited ventricular fibrosis to the same degree. The ACEI decreased the level of type I collagen mRNA, but the decrease was less than that induced by the ARB. The difference in collagen synthesis was probably cancelled out by that in degradation: both in-vitro and in-situ zymography showed that gelatinase activity was greater in the rats treated with the ACEI than in those treated with the ARB.
  
  
  CONCLUSIONS
  An ARB and an ACEI inhibited ventricular fibrosis through different mechanisms in hypertensive DHF.
authors.affiliation: Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Osaka University, Suita, Japan. kazushiro@medone.med.osaka-u.ac.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mano
authors.forename: Junichi
authors.initials: J
authors.lastname: Yoshida
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sakata
authors.forename: Nagahiro
authors.initials: N
authors.lastname: Nishikawa
authors.forename: Mayu
authors.initials: M
authors.lastname: Nishio
authors.forename: Tomohito
authors.initials: T
authors.lastname: Ohtani
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Hori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miwa
authors.forename: Tohru
authors.initials: T
authors.lastname: Masuyama
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D057911:Angiotensin Receptor Antagonists; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000627:therapeutic use*; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D024042:Collagen Type I / Q000187:drug effects; D004452:Echocardiography; D005355:Fibrosis / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D006333:Heart Failure / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D006352:Heart Ventricles / Q000473:pathology* / Q000503:physiopathology; D006439:Hemodynamics / Q000187:drug effects; D006973:Hypertension / Q000150:complications*; D008297:Male; D051381:Rats; D020303:Rats, Inbred Dahl; D013777:Tetrazoles / Q000494:pharmacology*; D013841:Thiazepines / Q000627:therapeutic use*; D018487:Ventricular Dysfunction, Left / Q000188:drug therapy
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D057911:Angiotensin Receptor Antagonists; D000806:Angiotensin-Converting Enzyme Inhibitors; D001562:Benzimidazoles; D001713:Biphenyl Compounds; D024042:Collagen Type I; D013777:Tetrazoles; D013841:Thiazepines; C055603:temocapril hydrochloride; C077793:candesartan cilexetil