title: Axenic culture of Gyrodinium impudicum strain KG03, a marine red-tide microalga that produces exopolysaccharide.
abstract: An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and designated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.
authors.affiliation: Korea Polar Research Institute, KORDI, Ansan P.O. Box 29, Seoul 425 600, Republic of Korea.
authors.forename: Joung Han
authors.initials: JH
authors.lastname: Yim
authors.forename: Hong Kum
authors.initials: HK
authors.lastname: Lee
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D046508:Culture Techniques; D004141:Dinoflagellida / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D056890:Eukaryota / Q000302:isolation & purification; D008386:Marine Biology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D011134:Polysaccharides; D012340:RNA, Ribosomal, 5.8S