Antibacterial activity of seed proteins of Robinia pseudoacacia.
A low molecular weight cationic peptide was isolated from Robinia pseudoacacia seed and tested in vitro against seven bacteria (Corynebacterium michiganense, Staphylococcus aureus, Bacillus subtilis, Erwinia carotovora subsp. carotovora, Pseudomonas syringae pv syringae, Xanthomonas campestris pv campestris, and Escherichia coli). The peptide inhibited the growth of the tested strains. The effective concentrations required for 50% inhibition of bacterial growth ranged between 20 and 120 microg ml(-1) protein. S. aureus was found to be the most sensitive strain, however, E. coli was not affected much when compared with others. Reduction of antibacterial activity of the peptide with CaCl2 addition into the growth medium was also observed.
Talas-Oğraş
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TUBITAK, Research Institute for Genetic Engineering and Biotechnology, P.O Box. 21 41470 Gebze-Kocaeli, Turkey. tijen@rigeb.gov.tr
Ipekçi
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Bajroviç
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Gözükirmizi
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D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010940:Plant Proteins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D031301:Robinia; D012639:Seeds
D000900:Anti-Bacterial Agents; D010940:Plant Proteins