title: Tumor recognition following Vgamma9Vdelta2 T cell receptor interactions with a surface F1-ATPase-related structure and apolipoprotein A-I.
abstract: Vgamma9Vdelta2 T lymphocytes, a major gammadelta T lymphocyte subset in humans, display cytolytic activity against various tumor cells upon recognition of yet uncharacterized structures. Here, we show that an entity related to the mitochondrial F1-ATPase is expressed on tumor cell surface and promotes tumor recognition by Vgamma9Vdelta2 T cells. When immobilized, purified F1-ATPase induces selective activation of this lymphocyte subset. The Vgamma9Vdelta2 T cell receptors (TCR) and the F1-ATPase also bind a delipidated form of apolipoprotein A-I (apo A-I), as demonstrated by surface plasmon resonance. Moreover, the presence of apo A-I in the culture medium is required for optimal activation of Vgamma9Vdelta2 T cells by tumors expressing F1-ATPase. This study thus describes an unanticipated tumor recognition mechanism by Vgamma9Vdelta2 lymphocytes and a possible link between gammadelta T cell immunity and lipid metabolism.
authors.affiliation: Inserm U601, Institut de Biologie, 9 quai Moncousu, F-44035 Nantes Cedex 01, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Scotet
authors.forename: Laurent O
authors.initials: LO
authors.lastname: Martinez
authors.forename: Ethan
authors.initials: E
authors.lastname: Grant
authors.forename: Ronald
authors.initials: R
authors.lastname: Barbaras
authors.forename: Paul
authors.initials: P
authors.lastname: Jenö
authors.forename: Martine
authors.initials: M
authors.lastname: Guiraud
authors.forename: Bernard
authors.initials: B
authors.lastname: Monsarrat
authors.forename: Xavier
authors.initials: X
authors.lastname: Saulquin
authors.forename: Sophie
authors.initials: S
authors.lastname: Maillet
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Estève
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lopez
authors.forename: Bertrand
authors.initials: B
authors.lastname: Perret
authors.forename: Xavier
authors.initials: X
authors.lastname: Collet
authors.forename: Marc
authors.initials: M
authors.lastname: Bonneville
authors.forename: Eric
authors.initials: E
authors.lastname: Champagne
mesh_terms: D016632:Apolipoprotein A-I / Q000378:metabolism*; D006801:Humans; D007103:Immobilization; D019169:Jurkat Cells; D007700:Kinetics; D008024:Ligands; D008213:Lymphocyte Activation; D009369:Neoplasms / Q000276:immunology*; D011485:Protein Binding; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D016632:Apolipoprotein A-I; D008024:Ligands; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D006180:Proton-Translocating ATPases