title: Scale-up of biopesticide production processes using wastewater sludge as a raw material.
abstract: Studies were conducted on the production of Bacillus thuringiensis (Bt)-based biopesticides to ascertain the performance of the process in shake flasks, and in two geometrically similar fermentors (15 and 150 l) utilizing wastewater sludge as a raw material. The results showed that it was possible to achieve better oxygen transfer in the larger capacity fermentor. Viable cell counts increased by 38-55% in the bioreactor compared to shake flasks. As for spore counts, an increase of 25% was observed when changing from shake flask to fermentor experiments. Spore counts were unchanged in bench (15 l) and pilot scale (5.3-5.5 e(+08) cfu/ml; 150 l). An improvement of 30% in the entomotoxicity potential was obtained at pilot scale. Protease activity increased by two to four times at bench and pilot scale, respectively, compared to the maximum activity obtained in shake flasks. The maximum protease activity (4.1 IU/ml) was obtained in pilot scale due to better oxygen transfer. The Bt fermentation process using sludge as raw material was successfully scaled up and resulted in high productivity for toxin protein yield and a high protease activity.
authors.affiliation: INRS Eau, Terre et Environnement (INRS-ETE), 2800 Rue Einstein, CP 7500, Sainte-Foy, QC, G1V4C7, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Yezza
authors.forename: R D
authors.initials: RD
authors.lastname: Tyagi
authors.forename: J R
authors.initials: JR
authors.lastname: Valèro
authors.forename: R Y
authors.initials: RY
authors.lastname: Surampalli
authors.forename: J
authors.initials: J
authors.lastname: Smith
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D019149:Bioreactors; D015169:Colony Count, Microbial; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D007814:Larva / Q000254:growth & development; D007915:Lepidoptera / Q000254:growth & development*; D010572:Pest Control, Biological; D015203:Reproducibility of Results; D012697:Serine Endopeptidases / Q000378:metabolism*; D012722:Sewage; D013171:Spores, Bacterial / Q000502:physiology; D014865:Waste Disposal, Fluid
chemical_list: D012722:Sewage; D012697:Serine Endopeptidases; C032923:microbial serine proteinases