[Effects of shuangshen tongguan (SSTG) on TNF-alpha, ICAM-1 during myocardial ischemia-reperfusion injury].
OBJECTIVE
To observe the effects of SSTG on infarction size and tumor necrosis factor-alpha (TNF-alpha), intercellular adhesion molecular-1 (ICAM-1) levels in serum during reperfusion injury of acute myocardial ischemia.


METHODS
Anterior descending branch of coronary artery was ligated and released to create myocardial ischemia-reperfusion injury. The size and weight of infarction area and the contents of TNF-alpha, IGAM-1 in serum were assayed by N-BT staining and ELISA respectively.


RESULTS
The size and weight of infarct area and the contents of TNF-alpha, ICAM-1 in serum were significantly increased compared with the normal group and were obviously decreased after being treated with SSIG.


CONCLUSIONS
Ischemia-reperfusion stimulated the secretion of TNF-alpha and ICAM-1, which play an important role in ischemia-reperfusion injury. SSTG might protect myocardium from ischemia-reperfusion injury by suppressing over-secretion of TNF-alpha and ICAM-1 and reducing the size and weight of infarction area.
Han
Xiao
X
Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China.
Liu
Jian-xun
JX
Ma
Xiao-bin
XB
Wang
Yang-hui
YH
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000097:blood* / Q000473:pathology; D009206:Myocardium / Q000473:pathology*; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1