Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method.
Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.
Song
Tianyan
T
Division of Bacterial Pathogenesis, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. k018763@med.u-ryukyu.ac.jp
Toma
Claudia
C
Nakasone
Noboru
N
Iwanaga
Masaaki
M
D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015342:DNA Probes; D003967:Diarrhea / Q000382:microbiology; D004405:Dysentery, Bacillary / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D012760:Shigella / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015342:DNA Probes; C063500:ipaH protein, Shigella flexneri