Investigation of artificial biomembrane systems in biopartitioning micellar chromatography by method of mathematical design.
An attempt to create and study an artificial membrane system was realized via biopartitioning micellar liquid chromatography. Towards this end the known formula of membrane permeability (on the basis of Fick's diffusion equation) was modified so that membrane permeability may be estimated in terms of chromatographic characteristics. The two-factoral experiments on the basis of mathematical design of second order were carried out. The regression equations are derived which describe the dependence of membrane permeability on the concentration of polyoxyethylene (23) lauryl ether in the mobile phase and its flow-rate for compounds with biomedical significance. Some regularities were revealed, which characterize the permeability of compounds of the different nature through membranes. The extremal dependence (with passing through minimum) of permeability on the concentration of non-ionic surfactant was observed for anionic compounds. The increasing character of permeability in relation with flow-rate of mobile phase was recognized for cationic samples. Both dependences were basically fulfilled for zwitterionic compounds.
Rukhadze
Marina D
MD
Department of Chemistry and Laboratory of Neurobiology, I. Javakhishvili Tbilisi State University, I. Chavchavadze Ave. 3, Tbilisi, 0128, Georgia. marina_rukhadze@yahoo.com
Bezarashvili
George S
GS
Kutkhashvili
Maya G
MG
Sigua
Kethevan I
KI
D000838:Anions; D001462:Barbital / Q000737:chemistry; D019817:Benzoic Acid / Q000737:chemistry; D002220:Carbamazepine / Q000737:chemistry; D002412:Cations; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000379:methods*; D004058:Diffusion; D004295:Dihydroxyphenylalanine / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008433:Mathematics; D008567:Membranes, Artificial; D008823:Micelles; D010424:Pentobarbital / Q000031:analogs & derivatives* / Q000737:chemistry; D010539:Permeability; D010649:Phenylalanine / Q000737:chemistry; D000077423:Polidocanol; D011092:Polyethylene Glycols; D012256:Riboflavin / Q000737:chemistry; D012439:Saccharin / Q000737:chemistry; D013501:Surface-Active Agents
D000838:Anions; D002412:Cations; D008567:Membranes, Artificial; D008823:Micelles; D013501:Surface-Active Agents; D000077423:Polidocanol; D002220:Carbamazepine; D011092:Polyethylene Glycols; D010649:Phenylalanine; D001462:Barbital; D004295:Dihydroxyphenylalanine; C055631:benzobamil; D019817:Benzoic Acid; D012439:Saccharin; D010424:Pentobarbital; D012256:Riboflavin