[The expression of p53, MDM2 and Ref1 gene in cultured retina neurons of SD rats treated with vitamin B1 and/or elevated pressure].
OBJECTIVE
To investigate the expression of p53, MDM2 and Ref1 gene in cultured retina neurons of SD rats treated with Vitamin B1 and (or) elevated pressure.


METHODS
The retinal neuron of postnatal SD rats were cultured in vivo, the elevated pressure was produced after 7 days, and the total RNA was extracted after another 2 days, expression of p53, MDM2 and Ref1 gene were analyzed with RT-PCR.


RESULTS
The expression level of p53 and MDM2 gene were increased in elevated pressure group, normal with Ref1 gene expression. But the expression of p53 and MDM2 gene were decreased significantly in elevated pressure group treated with vitamine B1 compare to the elevated group.


CONCLUSIONS
Apoptosis seem to be a mechanism of cell death in retinal neurons of SD rats with elevated pressure.Vitamine B1 have protect effects against elevated pressure.
Yang
Zhikuan
Z
Zhongshan Ophthalmic Center, Sun Yat-sen Univerity, Guangzhou 510060, China.
Ge
Jian
J
Yin
Wei
W
Shen
Huangxuan
H
Liu
Haiquan
H
Guo
Yan
Y
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics; D015870:Gene Expression; D016158:Genes, p53 / Q000235:genetics*; D011312:Pressure; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D012313:RNA / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013831:Thiamine / Q000494:pharmacology*
D012313:RNA; C497208:Mdm2 protein, rat; D051736:Proto-Oncogene Proteins c-mdm2; C470319:Apex1 protein, rat; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D013831:Thiamine