[Changes in level of organic acids in fructus crataegi after processing].
OBJECTIVE
To compare and analyze the organic acids level in fruit of Crataegus pinnatifida var. major before and after processing.


METHODS
The amount of total organic acids and individual citric acid, malate acid, ursolic acid and oleanolic acid in fruit of C. pinnatifida var. major before and after processing were determined by pH titration and HPLC analysis.


RESULTS
Good linear relationship between peakarea and amount was noted for 0.28-9.02 microg of malate acid, with a correlation coefficient of 0.9998. Regression equation was Y = 2344.7 + 49309.6 X. Good linear relationships between the logarithm of peak area and amount were noted for 0.348-1.74 microg of usolic acid and 0.498-2.48 microg of oleanolic acid, with a correlation coefficient of 0.9998 in each case. Regression equations were Y = 1.3628 X + 5.9508 and Y = 1.4447 X + 5.8236, respectively.


CONCLUSIONS
The lipid-soluble organic acids mostly remain whereas only about 70% of the water-soluble acids remain after processing in fruit of C. pinnatifida var. major.
Yang
Bin
B
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. ybinmm@hotmail.com
Li
Hua
H
Zhao
Yu-xin
YX
Li
Man-ling
ML
D019343:Citric Acid / Q000032:analysis; D027825:Crataegus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D006358:Hot Temperature; D008293:Malates / Q000032:analysis*; D009828:Oleanolic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical; D014315:Triterpenes / Q000032:analysis*; D000097245:Ursolic Acid
D019343:Citric Acid; D004365:Drugs, Chinese Herbal; D008293:Malates; D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid; C030298:malic acid