[Gene chip analysis of mutation of HBV-DNA open reading frame].
OBJECTIVE
To detect the mutation of HBV-DNA open reading frame (ORF), provide evidences for clinical diagnosis and treatment.


METHODS
By using gene chip technique, HBV-DNA was amplified by PCR, incorporated with fluorescence, hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF.


RESULTS
The mutations of HBV-DNA ORF existed widespread. The rates of mutation on Pre-C 1896, PreC 1814, BCP 1762, BCP 1764, P 528, P 552MI, P 552MV were 23.5%, 3.9%, 55.9%, 53.9%,39.2%, 38.2%, 10.8%, respectively.


CONCLUSIONS
The gene chip technique possesses extremely high sensitivity and reliability,it is one of the effective methods to detect gene mutation. The mutation of HBV-DNA has important influence on the stability and progress of the disease, and on the judgement of prognosis.
Chen
Shi-jun
SJ
Jinan Infectious Disease Hospital, Jinan 250021, China.
Du
Wen-jun
WJ
Lu
Yan-qin
YQ
Xu
Wei
W
An
Yong
Y
D004279:DNA, Viral / Q000235:genetics*; D006512:Hepatitis B Core Antigens / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016366:Open Reading Frames / Q000235:genetics*
D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens