Prolonged platelet preservation by transient metabolic suppression.
BACKGROUND
In this study whether metabolic suppression can be used to preserve platelet (PLT) function during prolonged storage was investigated.


METHODS
Washed human PLTs were incubated without glucose and with antimycin A to block energy generation. Metabolic suppressed PLTs (MSPs) were stored for 72 hours at different temperatures to find the optimal storage temperature. Controls were incubated with 5 mmol per L glucose and stored at 22 and 4 degrees C.


RESULTS
Following metabolic recovery with glucose, MSPs stored at 37, 22, and 4 degrees C showed an increase in basal P-selectin expression (PSE) reaching greater than 40 percent after about 2, 20, and 48 hours; a decrease in thrombin receptor-activating peptide SFLLRN (TRAP)-induced PSE inversely related to the increase in basal PSE; and a decrease in TRAP-induced aggregation reaching less than 30 percent after about 4, 24, and more than 72 hours. When compared with control suspensions, MSPs stored at 4 degrees C better preserved a low basal PSE and in addition showed a better adhesion to surface coated-von Willebrand factor and fibrinogen in a flow chamber.


CONCLUSIONS
Metabolic suppression before storage at 4 degrees C contributes to better preservation of PLT function.
Badlou
B A
BA
Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center Utrecht, the Netherlands.
Ijseldijk
M J W
MJ
Smid
W M
WM
Akkerman
J W N
JW
D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D003080:Cold Temperature; D004734:Energy Metabolism; D005340:Fibrinogen / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D019007:P-Selectin; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism; D017713:Platelet Transfusion; D014841:von Willebrand Factor / Q000378:metabolism
D019007:P-Selectin; D019038:Platelet Glycoprotein GPIb-IX Complex; D014841:von Willebrand Factor; D005340:Fibrinogen; D005947:Glucose