pH-dependent quenching of the fluorescence of tryptophan residues in class A beta-lactamase from E. coli (TEM-1).
We performed an investigation of the pH-dependent quenching of the fluorescence of tryptophan residues of TEM-1 beta-lactamase from E. coli by uncharged and charged quenchers. pH-dependent Stern-Volmer constants (Ksv/pH) of tryptophan residues allowed us to determine subtle but discrete structurally and functionally important processes.
Christov
Christo
C
Institute of Organic Chemistry Bulgarian Academy of Science, Sofia 1113, Bulgaria. christov@sg.uji.es
Ianev
Dimitar
D
Shosheva
Alexandra
A
Atanasov
Boris
B
D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013050:Spectrometry, Fluorescence / Q000379:methods; D014364:Tryptophan; D001618:beta-Lactamases / Q000737:chemistry* / Q000302:isolation & purification
D029968:Escherichia coli Proteins; D014364:Tryptophan; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1