title: The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes.
abstract: We describe developmentally regulated genes in two strains of Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria; of these, about 60 were novel. Only two genes -- encoding HSP83 and an importin-related protein -- appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level.
authors.affiliation: DKFZ, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Brems
authors.forename: D Lys
authors.initials: DL
authors.lastname: Guilbride
authors.forename: Drifa
authors.initials: D
authors.lastname: Gundlesdodjir-Planck
authors.forename: Christian
authors.initials: C
authors.lastname: Busold
authors.forename: Van-Duc
authors.initials: VD
authors.lastname: Luu
authors.forename: Michaela
authors.initials: M
authors.lastname: Schanne
authors.forename: Jörg
authors.initials: J
authors.lastname: Hoheisel
authors.forename: Christine
authors.initials: C
authors.lastname: Clayton
mesh_terms: D000818:Animals; D001769:Blood / Q000469:parasitology; D018507:Gene Expression Regulation, Developmental; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D020543:Proteome; D015800:Protozoan Proteins; D012333:RNA, Messenger