Column selectivity in reversed-phase liquid chromatography. VIII. Phenylalkyl and fluoro-substituted columns.
As reported previously, five solute-column interactions (hydrophobicity, steric resistance, hydrogen-bond acidity and basicity, ionic interaction) quantitatively describe column selectivity for 163 alkyl-silica, polar-group and cyano columns. In the present study, solute retention and column selectivity for 11 phenyl and 5 fluoro-substituted columns were compared with alkyl-silica columns of similar ligand length. It is concluded that two additional solute-column interactions may be significant in affecting retention and selectivity for the latter columns: (a) dispersion interactions of varying strength as a result of significant differences in bonded-phase polarizability or refractive index and (b) pi-pi interactions in the case of phenyl columns and aromatic solutes. These 16 phenyl and fluoro columns were also characterized in terms of hydrophobicity, steric resistance, hydrogen-bond acidity and basicity, and ionic interaction.
Marchand
D H
DH
Department of Chemistry, University Wisconsin - River Falls, River Falls, WI 54022, USA.
Croes
K
K
Dolan
J W
JW
Snyder
L R
LR
Henry
R A
RA
Kallury
K M R
KM
Waite
S
S
Carr
P W
PW
D002853:Chromatography, Liquid / Q000295:instrumentation*; D006860:Hydrogen Bonding; D012031:Refractometry; D012680:Sensitivity and Specificity