Prevention of postsurgical tissue adhesion by anti-inflammatory drug-loaded pluronic mixtures with sol-gel transition behavior.
Sol-gel transition temperature-controllable Pluronic F127/F68 mixtures including mildly crosslinked alginate and nonsteroidal anti-inflammatory drug (ibuprofen) were prepared to evaluate their potential as tissue adhesion barrier gels. The sol-gel transition temperatures of the Pluronic mixtures could be controlled by adjusting F127/F68 ratio and polymer concentration. The mildly crosslinked alginate with still flow property provided the residence stability of Pluronic mixture gels in the body. Ibuprofen was loaded in Pluronic mixtures to reduce inflammatory response in the body and, thus, to prevent tissue adhesion. The gelation temperatures of the Pluronic mixtures were not affected by the alginate but lowered by the addition of ibuprofen. The in vitro drug release behavior and in vivo peritoneal tissue adhesion of the Pluronic mixtures with the sol-gel transition just below body temperatures were investigated. The drug release behavior from the ibuprofen (1 wt%)-loaded Pluronic mixture gels at 37 degrees C was examined using a membrane-less dissolution model. The drug in the mixture gels was released continuously up to about 45-65% of the total loading amount during the first 7 days. For in vivo evaluation of tissue anti-adhesion potential, the Pluronic mixtures with/without drug were coated on the peritoneal wall defects of rats and their tissue adhesion extents and tissue reactions (inflammatory response, granulation tissue formation, and toxicity in organs) were compared. It was observed that ibuprofen has a positive effect for the peritoneal tissue anti-adhesion. The Pluronic F127/F68/alginate/ibuprofen mixture gel (25 wt% of F127/F68 [7/3], 1 wt% ibuprofen) was highly effective for the prevention of peritoneal tissue adhesion and showed a relatively low inflammatory response and non-toxicity, and thus can be a good candidate material as a coatable or injectable tissue adhesion barrier gel.
Oh
Se Heang
SH
Department of Polymer Science and Engineering, Hannam University, 133 Ojeong Dong, Daedeog Gu, Daejeon 306-791, Korea.
Kim
Jin Kyeong
JK
Song
Kyu Sang
KS
Noh
Seung Moo
SM
Ghil
Sung Ho
SH
Yuk
Soon Hong
SH
Lee
Jin Ho
JH
D000464:Alginates; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001672:Biocompatible Materials; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007052:Ibuprofen / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D010537:Peritoneum / Q000502:physiology / Q000601:surgery; D020442:Poloxamer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000187:drug effects; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D000464:Alginates; D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020442:Poloxamer; D020723:Glucuronic Acid; D007052:Ibuprofen