Effect of thermal injury on splenic myelopoiesis.
Thermal injury increases the number of macrophage progenitors in the bone marrow but leads to a decrease in the number of granulocyte progenitors. In the spleen, thermal injury increases the numbers of myeloid progenitors, but the lineage commitment of these cells is unknown. In this study mice were given a scald burn, and the number of splenic myeloid progenitors as well as their progeny was determined. BrdU uptake was used to monitor the de novo production of splenocytes for 8 days after the burn. Burn injury increased the numbers of splenic granulocyte-macrophage (GM), granulocyte (G), and macrophage (M) progenitors at postburn day 8 by 12-, 11-, and 18-fold, respectively. Scald injury increased the number of mature PMN (CD11b GR1(bright)) in the spleen and increased the number of white pulp monocyte/macrophages. Increased numbers of BrdU-positive PMN and monocyte/macrophages were seen after injury. Burn macrophages produced increased levels of the anti-inflammatory hematopoietic cytokine G-CSF. Our work clearly shows that the increased myelopoiesis observed postinjury leads to the production of mature myeloid cells. However, the effects of thermal injury on progenitors in the spleen and marrow are not equivalent.
Noel
John G
JG
Research, Shriners Hospital for Children, 3229 Burnet Avenue, Cincinnati, OH 45229, USA. waterdog02@aol.com
Guo
Xialing
X
Wells-Byrum
Denise
D
Schwemberger
Sandy
S
Caldwell
Charles C
CC
Ogle
Cora K
CK
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002056:Burns; D004396:Coloring Agents / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D006358:Hot Temperature; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000378:metabolism; D038042:Myelopoiesis; D013154:Spleen / Q000166:cytology* / Q000293:injuries / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D004396:Coloring Agents; D016207:Cytokines; D004791:Enzyme Inhibitors; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D001973:Bromodeoxyuridine