Calcium-modulated ciliary membrane guanylate cyclase transduction machinery: constitution and operational principles.
Odorant transduction is a biochemical process by which the odorant signal generates the electric signal. The cilia of the olfactory neuroepithelium are the sites of this process. This study documents the detailed biochemical, structural and functional description of an odorant-responsive Ca2+ -modulated membrane guanylate cyclase transduction machinery in the cilia. Myristoylated (myr)-neurocalcin delta is the Ca2+ -sensor component and the cyclase, ONE-GC, the transduction component of the machinery. Myr-neurocalcin delta senses increments in free Ca2+, binds to a defined domain of ONE-GC and stimulates the cyclase. The findings enable the formulation of an odorant transduction model in which three pivotal signaling components--Ca2+, myr-neurocalcin delta and ONE-GC--of the transduction machinery are locked. A glaring feature of the model is that its Ca2+ -dependent operational principle is opposite to the phototransduction model.
Duda
Teresa
T
The Unit of Regulatory and Molecular Biology, Department of Cell Biology, SOM & NJMS, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA.
Fik-Rymarkiewicz
Ewa
E
Venkataraman
Venkateswar
V
Krishnan
Anuradha
A
Sharma
Rameshwar K
RK
D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002522:Chlorocebus aethiops; D002923:Cilia / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D017353:Gene Deletion; D006162:Guanylate Cyclase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D051596:Neurocalcin; D009831:Olfactory Mucosa / Q000187:drug effects / Q000201:enzymology*; D017434:Protein Structure, Tertiary; D044169:Receptors, Calcium-Sensing; D011994:Recombinant Proteins / Q000494:pharmacology
D003432:Cross-Linking Reagents; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing; D011994:Recombinant Proteins; D006162:Guanylate Cyclase; D002118:Calcium