[Effect of Yigu capsule on NO and NOS of rat osteoblasts in vitro].
OBJECTIVE
The effects of Yigu Capsule on NO and NOS of osteoblasts were studied.


METHODS
(1) Forty 12-month old Sprague-daweley female rats were divided into randomly four groups (Yigu Capsule high dose group, medium dose group and low dose group; saline group), the drug-containing serum and control serum were prepared. (2) The new-born Sprague-daweley rat Osteoblasts were cultured with different Yigu Capsule drug-containing serum concentration and at different exposure time. The mRNA levels of eNOS and iNOS were measured by RT-PCR; the activities of eNOS and iNOS were measured by test kit at different exposure time. NO secretion of osteoblasts was observed, too.


RESULTS
The mRNA levels and the activities of eNOS and iNOS and NO secretion of osteoblasts were higher than the control groups respectively after 48,72 and 96 hours (P < 0.01 or P <0.05).


CONCLUSIONS
Yigu Capsule drug-containing serum can promote NOS mRNA and NOS activities expressions and NO secretion of osteoblasts. These results may be parts of the mechanism of Yigu Capsule preventing and treating osteoporosis.
Zhang
Ronghua
R
Jinan University, Guangzhou.
Wang
Tingchun
T
Zhu
Xiaofeng
X
Cai
Yu
Y
Peng
Keping
K
Zheng
Shifu
S
D000818:Animals; D002214:Capsules; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D002214:Capsules; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase