Three-dimensional chemical structures by protein functionalized micron-sized beads bound to polylysine-coated silicone surfaces.
A novel method is described here that allows three-dimensional (3D) control of both chemistry and morphology by a series of wet chemical steps: the attachment of protein functionalized micron-sized beads onto a flat silicone surface that has been functionalized with a distinct chemical modification. Bovine serum albumin (BSA), laminin, or polylysine is covalently bound to 6.5-microm-diameter spherical beads. A chemical method is then used to bind these beads to a flat silicone surface that is subsequently functionalized with polylysine. This process leads to a nonspecific cell adhesive background on the flat surface (polylysine) with the option of differing chemistry on the third-dimension due to the protein BSA or laminin on the bead protruding from the surface. The beads do not detach during cyclic stretching in vitro. Neo-natal rat cardiac fibroblasts are cultured on the beaded surfaces and compared with fibroblasts cultured on nonbeaded, flat polylysine surfaces. Fibroblast plating density, integrin, and physical responses are examined as a function of varying the ligands on the beads.
Lateef
Syed S
SS
Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
Boateng
Samuel
S
Ahluwalia
Neil
N
Hartman
Thomas J
TJ
Russell
Brenda
B
Hanley
Luke
L
D000818:Animals; D002448:Cell Adhesion; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D016023:Integrins / Q000502:physiology; D007797:Laminin / Q000737:chemistry; D008722:Methods; D008863:Microspheres; D008954:Models, Biological; D009206:Myocardium / Q000166:cytology; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D013499:Surface Properties; D046509:Tissue Culture Techniques
D016023:Integrins; D007797:Laminin; D012828:Silicones; D011107:Polylysine; D012710:Serum Albumin, Bovine