title: High dose of Garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis.
abstract: We investigated the ability of Garcinia cambogia extract containing (-)-hydroxycitric acid (HCA) to suppress body fat accumulation in developing male Zucker obese (fa/fa) rats. We also examined histopathologically the safety of its high doses. Diets containing different levels of HCA (0, 10, 51, 102 and 154 mmol/kg diet) were fed to 6-week-old rats for 92 or 93 days. Each diet group was pair-fed to the 154 mmol HCA/kg diet group. Epididymal fat accumulation and histopathological changes in tissues were observed. The highest dose of HCA-containing Garcinia cambogia (154 mmol HCA/kg diet) showed significant suppression of epididymal fat accumulation in developing male Zucker obese rats, compared with the other groups. However, the diets containing 102 mmol HCA/kg diet and higher (778 and 1244 mg HCA/kg BW/d, respectively) caused potent testicular atrophy and toxicity, whereas diets containing 51 mmol HCA/kg diet (389 mg HCA/kg BW/d) or less did not. Accordingly, 51 mmol HCA/kg diet (389 mg HCA/kg BW/d) was deemed to be the no observed adverse effect level (NOAEL).
authors.affiliation: Division of Food Science, Incorporated Administrative Agency, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan. msaito@nih.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Ueno
authors.forename: S
authors.initials: S
authors.lastname: Ogino
authors.forename: K
authors.initials: K
authors.lastname: Kubo
authors.forename: J
authors.initials: J
authors.lastname: Nagata
authors.forename: M
authors.initials: M
authors.lastname: Takeuchi
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000633:toxicity; D002951:Citrates / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D029761:Garcinia cambogia / Q000737:chemistry*; D020738:Leptin / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D011924:Rats, Zucker; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000097:blood; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D019440:Anti-Obesity Agents; D002951:Citrates; D020738:Leptin; D010936:Plant Extracts; D013739:Testosterone; C007999:hydroxycitric acid; D001275:ATP Citrate (pro-S)-Lyase