Confinement of transmembrane cell receptors in tunable stripe micropatterns.
We report a simple method to confine transmembrane cell receptors in stripe micropatterns of a lipid/lipopolymer monolayer, which are formed as result of the transfer onto a solid substrate. The stripes are aligned perpendicular to the meniscus, whose periodicity can systematically be tuned by the transfer velocity. This strongly suggests the dominant role of the cooperative interaction between the film and substrate. Selective fluorescence labeling of lipids and lipopolymers confirms that the observed patterns coincide with the demixing of two species. Covalent coupling of polymer headgroups enables us to use the stripe patterns as a support for a lipid bilayer membrane. Spreading of lipid vesicles with platelet integrin alphaIIbbeta3 on a self-assembled membrane micropattern demonstrates that cell adhesion receptors are selectively incorporated into the lipopolymer-rich region. The method established here provides us with a tunable template for the confinement of receptor proteins to geometrically control the cell adhesion.
Purrucker
Oliver
O
Lehrstuhl für Biophysik E22, Technische Universität München, James-Franck-Strasse, 85748 Garching, Germany.
Förtig
Anton
A
Lüdtke
Karin
K
Jordan
Rainer
R
Tanaka
Motomu
M
D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines / Q000737:chemistry; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000737:chemistry* / Q000378:metabolism; D014783:Viscosity
D008055:Lipids; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C021016:1,2-diphytanoylphosphatidylcholine; C027891:1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine