Curcumin stability and its effect on glutathione S-transferase P1-1 mRNA expression in K562 cells.
To investigate the stability of curcumin in physiological media, the absorption variation of a curcumin solution was measured in 0.1% and 10% FCS. Under daylight conditions, curcumin degraded very rapidly in 0.1% FCS and was found to be more stable in higher serum concentrations. Under dark conditions, almost no decomposition could be observed after 2 h, whether the measurements were performed in 0.1% or 10% FCS. Furthermore, depending on the medium concentration, differential glutathione S-transferase P1-1 mRNA expression could be observed in K562 cells after incubation with curcumin. Indeed, incubation in 0.1% FCS led to a decrease of mRNA expression, whereas incubation in 10% FCS induced an increase of mRNA production.
Blasius
Romain
R
Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hôpital Kirchberg, 9 rue Edward Steichen, L-2540 Luxembourg, Luxembourg.
Duvoix
Annelyse
A
Morceau
Franck
F
Schnekenburger
Michaël
M
Delhalle
Sylvie
S
Henry
Estelle
E
Dicato
Mario
M
Diederich
Marc
M
D001769:Blood; D015152:Blotting, Northern; D003470:Culture Media; D003474:Curcumin / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D012333:RNA, Messenger; D005982:Glutathione Transferase; D003474:Curcumin