title: On increasing protein-crystallization throughput for X-ray diffraction studies.
abstract: Two recent developments, a novel screening/optimization strategy that considerably reduces the number of trials required to produce diffraction-size crystals and a simple modification that doubles the screening capacity of the Douglas Instruments ORYX 1-6 protein-crystallization robot, have been implemented into a structural genomics project. The new two-step screening/optimization strategy yields diffraction-quality crystals directly from the screening process, reducing the need for further optimization. The ORYX modification involves the addition of extensions to the sample- and oil-delivery arms and software modifications that allow two plates to be set up simultaneously.
authors.affiliation: SouthEast Collaboratory for Structural Genomics, B202 Life Sciences, University of Georgia, Georgia 30602, USA.
authors.forename: Ashit K
authors.initials: AK
authors.lastname: Shah
authors.forename: Zhi Jie
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Patrick D
authors.initials: PD
authors.lastname: Stewart
authors.forename: Florian D
authors.initials: FD
authors.lastname: Schubot
authors.forename: John P
authors.initials: JP
authors.lastname: Rose
authors.forename: M Gary
authors.initials: MG
authors.lastname: Newton
authors.forename: Bi Cheng
authors.initials: BC
authors.lastname: Wang
mesh_terms: D003460:Crystallization; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D011506:Proteins