title: Rapid and selective micellar electrokinetic chromatography for simultaneous determination of amikacin, kanamycin A, and tobramycin with UV detection and application in drug formulations.
abstract: A simple and selective micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous determination of amikacin, tobramycin, and kanamycin A, performed in Tris buffer (180 mM; pH 9.1) with 300 mM sodium pentanesulfonate (SPS) as an anionic surfactant. Under this condition, good separation with high efficiency and the required short analysis time is achieved. The linear ranges of the method for the determination of amikacin, tobramycin, and kanamycin A were 0.1-0.5 mg / mL, 0.4-2.0 mg / mL, and 0.4-2.0 mg / mL, respectively; the detection limits (signal-to-noise ratio = 3; injection, 0.5 psi 5 s) were 0.08, 0.2, and 0.2 mg / mL, respectively. The small amount of sample required and the expeditiousness of the procedure allow content uniformity to be determined in individual commercial products.
authors.affiliation: Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University.
authors.forename: Hsin-Hua
authors.initials: HH
authors.lastname: Yeh
authors.affiliation: Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University.
authors.forename: Shun-Jin
authors.initials: SJ
authors.lastname: Lin
authors.affiliation: School of Medicine, Kaohsiung Medical University.
authors.forename: Ju-Yun
authors.initials: JY
authors.lastname: Ko
authors.affiliation: School of Medicine, Kaohsiung Medical University.
authors.forename: Chia-An
authors.initials: CA
authors.lastname: Chou
authors.affiliation: Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University.|Department of Pharmacy, Kaohsiung Medical University, Chung-Ho Memorial Hospital, Kaohsiung, Taiwan.
authors.forename: Su-Hwei
authors.initials: SH
authors.lastname: Chen
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry; D000583:Amikacin / Q000032:analysis / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007612:Kanamycin / Q000032:analysis / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014031:Tobramycin / Q000032:analysis / Q000302:isolation & purification*; D014325:Tromethamine
chemical_list: D017738:Alkanesulfonic Acids; D004364:Pharmaceutical Preparations; D014325:Tromethamine; C086946:1-pentanesulfonic acid; D007612:Kanamycin; D000583:Amikacin; D014031:Tobramycin