On increasing protein-crystallization throughput for X-ray diffraction studies.
Two recent developments, a novel screening/optimization strategy that considerably reduces the number of trials required to produce diffraction-size crystals and a simple modification that doubles the screening capacity of the Douglas Instruments ORYX 1-6 protein-crystallization robot, have been implemented into a structural genomics project. The new two-step screening/optimization strategy yields diffraction-quality crystals directly from the screening process, reducing the need for further optimization. The ORYX modification involves the addition of extensions to the sample- and oil-delivery arms and software modifications that allow two plates to be set up simultaneously.
Shah
Ashit K
AK
SouthEast Collaboratory for Structural Genomics, B202 Life Sciences, University of Georgia, Georgia 30602, USA.
Liu
Zhi Jie
ZJ
Stewart
Patrick D
PD
Schubot
Florian D
FD
Rose
John P
JP
Newton
M Gary
MG
Wang
Bi Cheng
BC
D003460:Crystallization; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
D011506:Proteins