title: Crystal structure of Mycobacterium tuberculosis D-3-phosphoglycerate dehydrogenase: extreme asymmetry in a tetramer of identical subunits.
abstract: Phosphoglycerate dehydrogenases exist in at least three different structural motifs. The first D-3-phosphoglycerate dehydrogenase structure to be determined was from Escherichia coli and is a tetramer composed of identical subunits that contain three discernable structural domains. The crystal structure of D-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis has been determined at 2.3 A. This enzyme represents a second structural motif of the D-3-phosphoglycerate dehydrogenase family, one that contains an extended C-terminal region. This structure is also a tetramer of identical subunits, and the extended motif of 135 amino acids exists as a fourth structural domain. This intervening domain exerts quite a surprising characteristic to the structure by introducing significant asymmetry in the tetramer. The asymmetric unit is composed of two identical subunits that exist in two different conformations characterized by rotation of approximately 180 degrees around a hinge connecting two of the four domains. This asymmetric arrangement results in the formation of two different and distinct domain interfaces between identical domains in the asymmetric unit. As a result, the surface of the intervening domain that is exposed to solvent in one subunit is turned inward in the other subunit toward the center of the structure where it makes contact with other structural elements. Significant asymmetry is also seen at the subunit level where different conformations exist at the NAD-binding site and the putative serine-binding site in the two unique subunits.
authors.affiliation: Department of Biochemistry & Biophysics, Texas A & M University, College Station, Texas 77843, USA.
authors.forename: Sanghamitra
authors.initials: S
authors.lastname: Dey
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Grant
authors.forename: James C
authors.initials: JC
authors.lastname: Sacchettini
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000502:physiology*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D050543:Phosphoglycerate Dehydrogenase; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012997:Solvents / Q000494:pharmacology
chemical_list: D008024:Ligands; D012997:Solvents; D002237:Carbohydrate Dehydrogenases; D050543:Phosphoglycerate Dehydrogenase