title: Formation of the B cell synapse: retention or recruitment?
abstract: Interaction of B cells with membrane antigen results in the formation of the B cell synapse: the B cell receptor (BCR) and antigen concentrate in the contact zone while CD45/B220 and the phosphatase SHP-1 are excluded. This study shows that, unlike in T cells, synapse formation does not require active transport processes (while subsequent antigen extraction and IgM downregulation do). The synapse architecture depends on the available protein ligands in the contact zone. Thus Syk, IgM and Fc receptor accumulation require the presence of ITAM-bearing BCRs, membrane antigen and membrane (IgG-containing) immune complexes, respectively. Remarkably, non-bound proteins are frequently not only homogeneously distributed but excluded from the contact zone. These results suggest that proteins mainly reach the contact zone by undirected diffusion, and in order not to be expelled by molecular crowding they require capture by and fixation to a binding protein.
authors.affiliation: Medical Research Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, United Kingdom. iber@maths.ox.ac.uk
authors.forename: D
authors.initials: D
authors.lastname: Iber
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D004792:Enzyme Precursors / Q000378:metabolism / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000378:metabolism / Q000502:physiology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D011961:Receptors, Fc / Q000276:immunology / Q000378:metabolism; D000072377:Syk Kinase
chemical_list: D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse