Human monoclonal antibodies that neutralize anthrax toxin by inhibiting heptamer assembly.
A panel of human anti-anthrax protective antigen IgG1 monoclonal antibodies were evaluated to determine the mechanism of toxin neutralization. AVP-22G12, AVP-1C6 and AVP-21D9 bound to the protective antigen with picomolar affinities to distinct non-overlapping linear epitopes. Two of the antibodies neutralized the anthrax toxin by completely inhibiting the protective antigen oligomer assembly process in vitro.
Wang
Fei
F
Avanir Pharmaceuticals, Antibody Technology, 11388 Sorrento Valley Rd, San Diego, CA 92121, USA. fwang@avanir.com
Ruther
Paul
P
Jiang
Ivy
I
Sawada-Hirai
Ritsuko
R
Sun
Shu Man
SM
Nedellec
Rebecca
R
Morrow
Phillip R
PR
Kang
Angray S
AS
D000911:Antibodies, Monoclonal / Q000276:immunology*; D061067:Antibodies, Monoclonal, Humanized; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D000080908:Broadly Neutralizing Antibodies; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009500:Neutralization Tests
C000601130:AVP-21D9; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D057134:Antibodies, Neutralizing; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000080908:Broadly Neutralizing Antibodies; C030325:anthrax toxin