title: Fructose metabolizing enzymes from mouse liver: influence of age and caloric restriction.
abstract: The influence of caloric restriction (CR) on the activities of liver fructose metabolizing enzymes and metabolite levels were studied in young (3 months) and old (30 months) mice. Fructokinase activity was increased (P<0.05) in both young and old CR mice when compared to controls while triokinase activity was increased (P<0.05) only in old CR versus control mice. Aldolase was not altered by CR in either old or young mice. No age-related differences in activities were observed in controls although a trend towards an increase was observed for triokinase, while significant age-related increases were observed for fructokinase and triokinase, but not aldolase, in CR mice. Both young and old mice on CR showed significant decreases in fructose and fructose-1-phosphate, however, no age-related changes in metabolite levels were observed for either control or CR mice. A fructose-1-phosphate kinase activity was also measured and found to be unchanged in both young and old mice on CR, but the activity was significantly lower in the old mice compared with young. We show here that the enzymes involved in fructose metabolism are influenced by CR and that this could contribute to alterations in gluconeogenesis and glycolysis observed with CR.
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, One Shields Ave., Davis, CA 95616, USA. khagopian@ucdavis.edu
authors.forename: Kevork
authors.initials: K
authors.lastname: Hagopian
authors.forename: Jon J
authors.initials: JJ
authors.lastname: Ramsey
authors.forename: Richard
authors.initials: R
authors.lastname: Weindruch
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000367:Age Factors; D000818:Animals; D031204:Caloric Restriction; D002352:Carrier Proteins / Q000502:physiology; D005632:Fructose / Q000032:analysis / Q000378:metabolism*; D005636:Fructosephosphates / Q000032:analysis / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D005636:Fructosephosphates; C499483:Gckr protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; C080906:glucokinase regulatory protein; C032284:fructose-1-phosphate; D005632:Fructose