Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen.
We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galbeta1-3(Fucalpha1-4)GlcNAcbeta1-) occurs in the N-glycans of the pollen allergens.
Kimura
Yoshinobu
Y
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Okayama, Japan. yosh8mar@cc.okayama-u.ac.jp
Kamamoto
Maiko
M
Maeda
Megumi
M
Okano
Mitsuhiro
M
Yokoyama
Minehiko
M
Kino
Kosuke
K
D000485:Allergens / Q000737:chemistry*; D052179:Antigens, Plant; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis*; D018653:Juniperus / Q000737:chemistry / Q000276:immunology*; D007983:Lewis Blood Group Antigens; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology
D000485:Allergens; D052179:Antigens, Plant; D000939:Epitopes; D007983:Lewis Blood Group Antigens; C494219:Lewis a oligosaccharide; D009844:Oligosaccharides; D010940:Plant Proteins; D011134:Polysaccharides; C121951:jun a 1 protein, Juniperus ashei