title: Transformation of Escherichia coli mediated by natural phospholipids.
abstract: Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCl2 or CaCl2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb).
authors.affiliation: Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University, Ibaraki, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kumazawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Yasurou
authors.initials: Y
authors.lastname: Kurusu
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D010714:Phosphatidylethanolamines / Q000494:pharmacology*; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine