Rapid enantioseparation of 1-aminoindan by microchip electrophoresis with linear-imaging UV detection.
Chiral separations of 1-aminoindan (AI) by cyclodextrin electrokinetic chromatography (CDEKC) were investigated on microfluidic quartz chips. By using a microchip electrophoresis (MCE) instrument equipped with a linear-imaging UV detector, the separation process of the enantiomeric compounds was observed. When sulfated beta-cyclodextrin was employed as a chiral selector, the baseline separation of AI could be achieved within 1 min with a high repeatability. The relative standard deviation of the migration time was less than 6%. The fastest separation was achieved in 14 s, utilizing a separation length of only 6.1 mm. These results show that the MCE analysis employing a linear imaging UV detector has a significant potential for fast chiral analysis.
Kitagawa
Fumihiko
F
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. fkitagawa@mbox.kudpc.kyoto-u.ac.jp
Aizawa
Shiori
S
Otsuka
Koji
K
D046209:Electrophoresis, Microchip; D007189:Indans / Q000737:chemistry / Q000302:isolation & purification*
D007189:Indans; C016992:1-aminoindan