Acid hydrolysis of protein in a microcapillary tube for the recovery of tryptophan.
The acid hydrolysis of proteins was miniaturized and simplified by employing microcapillary tubes (100 microl in volume) with 6 M HCl containing 1% 2-mercaptoethanol and 3% phenol for an amino acid compositional analysis. The method not only eliminated the laborious evacuation step for the hydrolysis tube but also decreased the destruction of tryptophan during hydrolysis. The recovery of tryptophan was 79% by acid hydrolysis at 145 degrees C for 4 h. Since the acid mixture could be removed under vacuum, the hydrolysate was subjected to an amino acid analysis without neutralization or dilution.
Adebiyi
Abayomi Peter
AP
Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Jin
Dong-Hao
DH
Ogawa
Tomohisa
T
Muramoto
Koji
K
D000596:Amino Acids / Q000737:chemistry; D006851:Hydrochloric Acid; D006868:Hydrolysis; D008623:Mercaptoethanol; D008832:Microchemistry; D019800:Phenol; D011506:Proteins / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry*
D000596:Amino Acids; D011506:Proteins; D019800:Phenol; D008623:Mercaptoethanol; D014364:Tryptophan; D006851:Hydrochloric Acid