Calpain-10 gene polymorphisms and type 2 diabetes in West Africans: the Africa America Diabetes Mellitus (AADM) Study.
OBJECTIVE
To investigate whether the three single nucleotide polymorphisms (SNPs), SNP-43, -56, and -63 of CAPN10 were associated with type 2 diabetes in a West African cohort.


METHODS
A total of 347 diabetic subjects and 148 unaffected controls from four ethnic groups in two West African countries were enrolled in this study. After genotyping three SNPs of CAPN10 and one SNP from CYP19, the allele, genotype, and haplotype frequencies as well as the odds ratios were calculated to test their association with type 2 diabetes.


RESULTS
None of the alleles or genotypes was associated with type 2 diabetes. Although statistical analysis indicated that haplotype 221 was associated with type 2 diabetes (OR, 3.765; 95% CI, 1.577-8.989) in the two ethnic groups of Nigeria, the same haplotype did not show any association with type 2 diabetes in the two ethnic groups in Ghana (OR, 0.906; 95% CI, 0.322-2.552).


CONCLUSIONS
Considering the relatively low frequency of haplotype 221 and that none of the haplotypes including 221 was associated with any of the diabetes-related quantitative traits tested, it is concluded that SNP-43, -56, and -63 of the CAPN10 gene variants may play a limited role in the risk of type 2 diabetes risks in this cohort of West Africans.
Chen
Yuanxiu
Y
College of Medicine, National Human Genome Center at Howard University, Washington, DC 20059, USA.
Kittles
Rick
R
Zhou
Jie
J
Chen
Guanjie
G
Adeyemo
Adebowale
A
Panguluri
Ramesh K
RK
Chen
Weidong
W
Amoah
Albert
A
Opoku
Victoria
V
Acheampong
Joseph
J
Agyenim-Boateng
Kofi
K
Eghan
Benjamin Ackon
BA
Nyantaki
Awuah
A
Oli
Johnnie
J
Okafor
Godfrey
G
Ofoegbu
Esther
E
Osotimehin
Babatunde
B
Abbiyesuku
Fayeofori
F
Johnson
Thomas
T
Fasanmade
Olufemi
O
Rufus
Theresa
T
Furbert-Harris
Paulette
P
Daniel
Harold I
HI
Berg
Kate A
KA
Collins
Francis S
FS
Dunston
Georgia M
GM
Rotimi
Charles N
CN
D000483:Alleles; D044383:Black People / Q000235:genetics; D002154:Calpain / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005869:Ghana / Q000453:epidemiology; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D009549:Nigeria / Q000453:epidemiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
D002154:Calpain; C415197:calpain 10