title: Full NMR assignment and revised structure for the capsular polysaccharide from Streptococcus pneumoniae type 15B.
abstract: The capsular polysaccharide from Streptococcus pneumoniae Type 15B is a component of the 23-valent polysaccharide vaccine against pneumococcal disease. We report full NMR assignments for the native and de-O-acetylated polysaccharide, and confirm that the phosphorylated substituent is glycerol-2-phosphate rather than phosphocholine, located on O-3 of the side chain beta-Galp residue. The polysaccharide is O-acetylated on the terminal alpha-Gal residue, distributed between O-2, O-3, O-4 and O-6 in a ratio of 6:12:12:55, with approximately 15% of the repeat units not O-acetylated.
authors.affiliation: Laboratory for Molecular Structure, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts EN6 3QG, UK. cjones@nibsc.ac.uk
authors.forename: Christopher
authors.initials: C
authors.lastname: Jones
authors.forename: Xavier
authors.initials: X
authors.lastname: Lemercinier
mesh_terms: D000107:Acetylation; D016667:Bacterial Capsules / Q000737:chemistry*; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000737:chemistry*
chemical_list: D011135:Polysaccharides, Bacterial; C503193:capsular polysaccharide, Streptococcus pneumoniae type 15B