Dietary vitamin E does not inhibit the promotion of liver carcinogenesis by polychlorinated biphenyls in rats.
In this study, the effect of dietary vitamin E on the hepatic tumor-promoting activity of PCB-77 and PCB-153 in female Sprague-Dawley rats (175-200 g) was investigated. One week after diethylnitrosamine injection, rats were fed purified diets containing 10, 50, or 250 mg/kg vitamin E in the form of alpha-tocopheryl acetate. Starting 1 wk later, we injected rats i.p. with vehicle (corn oil) or PCB-77 or PCB-153 (300 mumol/kg) every 14 d for 4 injections. All rats were killed 10 d after the last PCB injection. The number and volume of placental glutathione S-transferase (PGST)-positive foci were increased by PCB-77 but not by PCB-153. Vitamin E did not affect the induction of PGST-positive foci. PCB-77, but not PCB-153, increased hepatic NF-kappaB activity. In conclusion, dietary vitamin E supplementation does not protect against the induction of altered hepatic focal lesions by PCBs.
Glauert
Howard P
HP
Graduate Center for Nutritional Sciences, University of Kentucky, Lexington, KY 40506, USA. hglauert@uky.edu
Lu
Zijing
Z
Kumar
Amita
A
Bunaciu
R Petruta
RP
Patel
Sandip
S
Tharappel
Job C
JC
Stemm
Divinia N
DN
Lehmler
Hans-Joachim
HJ
Lee
Eun Y
EY
Robertson
Larry W
LW
Spear
Brett T
BT
D000818:Animals; D004032:Diet; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000517:prevention & control*; D016328:NF-kappa B / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
D016328:NF-kappa B; D014810:Vitamin E; D011078:Polychlorinated Biphenyls