Effect of Haimiding on the functioning of red cell membrane of FC and H22 tumor-bearing mice.
OBJECTIVE
To study the effect of Haimiding on the functioning of red cell membrane of FC and H(22) tumor-bearing mice.


METHODS
The membrane fluidity of red cells is measured with DPH fluorescence probe as a marker; the amount of red cell membrane proteins is measured using polyacrylamide gel electrophoresis; the amount of sialic acid (SA) on the surface of red cell membrane and the sealability of these cells are measured using colorimetric analysis.


RESULTS
Haimiding can lower the membrane fluidity of red cells in tumor-bearing mice and the amount of their membrane proteins, while increasing the amount of sialic acid in the membrane of red cells in these mice and enhancing the ability of the membrane of their red cells to reseal.


CONCLUSIONS
The anti-tumor effect of Haimiding on tumor-bearing mice is due to its ability to improve and restore the functions of the membrane of their red cell and to enhance the immune effect of the organisms.
Ji
Yu-Bin
YB
Postdoctoral Research Station, The Institute of Materia Medica, Harbin Commercial University, 138 Tongda Street, Daoli District, Harbin 150076, Heilongjiang Province, China. sygao2002@sina.com
Gao
Shi-Yong
SY
Cheng
Wei-Ping
WP
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D008113:Liver Neoplasms / Q000188:drug therapy*; D008297:Male; D008516:Medicine, Chinese Traditional; D008560:Membrane Fluidity / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009368:Neoplasm Transplantation
D000972:Antineoplastic Agents, Phytogenic; D008565:Membrane Proteins; D019158:N-Acetylneuraminic Acid