Detection of interleukin-6 in vaginal secretions of women with preterm premature rupture of membranes and its association with neonatal infection: a rapid immunochromatographic test.
OBJECTIVE
The purpose of this study was to evaluate the diagnostic value of an interleukin-6 (IL-6) bedside test of vaginal secretions for neonatal infection in cases of preterm premature rupture of membranes.


METHODS
This prospective clinical study included 73 patients. Interleukin-6 protein in vaginal secretions was determined with an immunochromatographic bedside test in <20 minutes.


RESULTS
Elevated C-reactive protein level (>20 mg/dL; odds ratio, 5.1; 95% CI, 0.9-28.6) and positive interleukin-6 level (odds ratio, 4.6; 95% CI, 1.2-18.4) were both associated with neonatal infection. After adjustment, only interleukin-6 remained associated with neonatal infection (odds ratio, 4.5; 95% CI, 1.1-18.5). The sensitivity of interleukin-6 for the prediction of neonatal infection was 79% (95% CI, 65-92); its specificity was 56% (95% CI, 42-70); its positive predictive value was 30% (95% CI, 12-47), and its negative predictive value was 92% (95% CI, 84-99).


CONCLUSIONS
Interleukin-6 protein determination by this new immunochromatographic test is a noninvasive prenatal vaginal marker of neonatal infection.
Kayem
Gilles
G
Department of Obstetrics and Gynecology, Cochin-Saint Vincent-de-Paul Hospital, Univerisity Paris V, France. gilles.kayem@chicreteil.fr
Goffinet
François
F
Batteux
Frédéric
F
Jarreau
Pierre H
PH
Weill
Bernard
B
Cabrol
Dominique
D
D000328:Adult; D001424:Bacterial Infections / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D002821:Chorioamnionitis / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes; D005602:France / Q000453:epidemiology; D006801:Humans; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000032:analysis* / Q000235:genetics; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D011296:Prenatal Diagnosis; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis; D014621:Vagina / Q000378:metabolism*
D015850:Interleukin-6; D012333:RNA, Messenger