title: Herpes B-virus seroreactivity in a colony of Macaca mulatta: data from the Sabana Seca Field Station, a new specific pathogen-free program.
abstract: The demand for B-virus-free animals for biomedical research is increasing, while at the same time the availability of such animals is decreasing. The establishment of Specific Pathogen-Free (SPF) breeding macaque colonies is a priority of the National Institutes of Health. Nevertheless, it is well known that seroreactivity to B-virus can be difficult to interpret, particularly as it can vary over time in a single animal. The aim of the present study was to implement a reliable algorithm to examine B-virus reactivity among the rhesus monkey population of the Caribbean Primate Research Center. The sensitivity and specificity of our assay were determined using reports from two different laboratories as references. Whereas 95.4% of animals showed consistent serological status and 4.6% of animals recruited to this SPF program showed serovariability to B-virus over the initial 2 years of examination. Implications for all SPF programs are discussed in this article.
authors.affiliation: Caribbean Primate Research Center, Unit of Comparative Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, PR 00936, USA. csariol@rcm.upr.edu
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Sariol
authors.forename: Teresa
authors.initials: T
authors.lastname: Arana
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Maldonado
authors.forename: Melissa
authors.initials: M
authors.lastname: Gerald
authors.forename: Janis
authors.initials: J
authors.lastname: Gonzalez-Martinez
authors.forename: Maria
authors.initials: M
authors.lastname: Rodriguez
authors.forename: Edmundo N
authors.initials: EN
authors.lastname: Kraiselburd
mesh_terms: D000465:Algorithms; D000822:Animal Husbandry; D000818:Animals; D001947:Breeding / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006566:Herpesviridae Infections / Q000662:veterinary*; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology*; D008137:Longitudinal Studies; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000276:immunology / Q000821:virology*; D009316:National Institutes of Health (U.S.); D011647:Puerto Rico; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013047:Specific Pathogen-Free Organisms; D014481:United States