title: Characterization of a truncated lipoarabinomannan from the Actinomycete Turicella otitidis.
abstract: Lipoarabinomannan (LAM) lipoglycans have been characterized from a range of mycolic acid-containing actinomycetes and from the amycolate actinomycete Amycolatopsis sulphurea. To further understand the structural diversity of this family, we have characterized the lipoglycan of the otic commensal Turicella otitidis. T. otitidis LAM (TotLAM) has been determined to consist of a mannosyl phosphatidylinositol anchor unit carrying an (alpha 1-->6)-linked mannan core and substituted with terminal-arabinosyl branches. Thus, TotLAM has a novel truncated LAM structure. Using the human monocytic THP-1 cell line, it was found that TotLAM exhibited only minimal ability to induce tumor necrosis factor alpha. These findings contribute further to our understanding of actinomycete LAM diversity and allow further speculation as to the correlation between LAM structure and the immunomodulatory activities of these lipoglycans.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale du Centre National de la Recherche Scientifique, Toulouse, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Gilleron
authors.forename: Natalie J
authors.initials: NJ
authors.lastname: Garton
authors.forename: Jérôme
authors.initials: J
authors.lastname: Nigou
authors.forename: Thérèse
authors.initials: T
authors.lastname: Brando
authors.forename: Germain
authors.initials: G
authors.lastname: Puzo
authors.forename: Iain C
authors.initials: IC
authors.lastname: Sutcliffe
mesh_terms: D039903:Actinobacteria / Q000737:chemistry*; D007294:Inositol / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C050016:lipoarabinomannan; D007294:Inositol