Stimulation of IL-8 production by Aralia cordate lectin in human colon carcinoma Caco-2 cells.
Some lectins are known to stimulate interleukin-8 (IL-8) productions in human colon carcinoma Caco-2 cells. Since IL-8 may cause deleterious effects, we examined this stimulatory activity of Aralia cordate lectin (ACL) and Wasabia japonica lectin (WJL), both of which we isolated recently. The results indicate that ACL exhibited strong stimulatory activity for IL-8 protein production, while WJL showed marginal activity. The activity of ACL was associated with high enhancement of IL-8 gene expression. The effect of ACL was abolished almost completely in the presence of brefeldin A, indicating that internalization into cells is necessary for ACL to exert activity. The findings suggest that ingestion of a large amount of raw vegetable Aralia cordate might cause unfavorable effects on the colon.
Koyama
Yu
Y
School of Food and Nutritional Sciences and Center of Excellence for the 21st Century, University of Shizuoka, Shizuoka, Japan.
Suzuki
Takuji
T
Kajiya
Akane
A
Isemura
Mamoru
M
D027983:Aralia / Q000737:chemistry*; D020126:Brefeldin A / Q000494:pharmacology; D018938:Caco-2 Cells; D003106:Colon / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D037121:Plant Lectins / Q000494:pharmacology*; D012333:RNA, Messenger; D031228:Wasabia / Q000737:chemistry
D016209:Interleukin-8; D037121:Plant Lectins; D012333:RNA, Messenger; D020126:Brefeldin A