title: Role of adrenergic receptors in the reflex diuresis in rabbits during pulmonary lymphatic obstruction.
abstract: The role of adrenergic receptors in the reflex diuresis in response to pulmonary lymphatic drainage was examined in anaesthetized, artificially ventilated New Zealand White rabbits. Pulmonary lymphatic drainage was obstructed by raising the pressure in a pouch created from the right external jugular vein. This pulmonary lymphatic obstruction results in a reflex increase in urine flow and sodium excretion. This reflex is abolished by renal denervation and by administration of L-NAME, a non-selective inhibitor of nitric oxide synthase. Also, infusion of the relatively selective neuronal nitric oxide synthase blocker, 7-nitroindazole sodium salt, into the renal medulla abolished the reflex diuresis. In this study the effects of adrenergic receptor antagonists on the reflex increase in urine were observed. Both ureters were cannulated in order to determine urine flow from both kidneys separately. Prazosin, an alpha1 adrenergic receptor antagonist, was infused into the renal medulla of the right kidney, while the left kidney acted as control. Administration of prazosin in this manner did not block the reflex diuresis in response to pulmonary lymphatic obstruction in either kidney. However, rauwolscine, an alpha2 adrenergic receptor antagonist, abolished the reflex increase in urine and sodium excretion in the ipsilateral kidney while preserving it in the contralateral kidney. These findings suggest that the increase in urine flow in rabbits caused by pulmonary lymphatic obstruction is dependent upon activation of alpha2 adrenergic receptors within the renal medulla.
authors.affiliation: Division of Cardiovascular Medicine, Bioletti Way, TB 172, University of California, Davis, CA 95616, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: McCormick
authors.forename: E M
authors.initials: EM
authors.lastname: Bravo
authors.forename: C T
authors.initials: CT
authors.lastname: Kappagoda
mesh_terms: D000818:Animals; D004231:Diuresis; D007679:Kidney Medulla / Q000294:innervation* / Q000503:physiopathology*; D008171:Lung Diseases / Q000150:complications / Q000503:physiopathology*; D008206:Lymphatic Diseases / Q000150:complications / Q000503:physiopathology*; D008954:Models, Biological; D011817:Rabbits; D011941:Receptors, Adrenergic / Q000378:metabolism*; D012018:Reflex
chemical_list: D011941:Receptors, Adrenergic