title: Relative expression of Pseudomonas aeruginosa virulence genes analyzed by a real time RT-PCR method during lung infection in rats.
abstract: The bacterial aspects of the events occurring during the first days of lung colonization by Pseudomonas aeruginosa are still unknown. The aim of this study was to develop a real time RT-PCR method for the direct quantification of the transcripts of three P. aeruginosa virulence genes: exoS, lasI and algD, during the first seven days of a rat lung infection. Our results document differences in bacterial gene expression throughout P. aeruginosa infection. ExoS transcripts levels were very high in the first days of infection, but a significant decrease was then progressively observed. Transcription of algD occurred on the 4th day and increased regularly over time suggesting a balance in the transcription of exoS and algD. The strong expression of exoS during the first 3 days was correlated to 29% of mortality among infected rats. On the contrary, the increase of algD expression after this period was associated to the development of a chronic infection with no further mortality. LasI transcription remained more constant throughout the infection.
authors.affiliation: EA 3925, Laboratoire de Bactériologie-Hygiène, Hôpital Calmette, Unité de Gastroentérologie, Hépatologie et Nutrition, Clinique de Pédiatrie, Hôpital Jeanne de Flandre, CHRU, 59037 Lille, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Joly
authors.forename: Joly
authors.initials: J
authors.lastname: Béatrice
authors.forename: Maud
authors.initials: M
authors.lastname: Pierre
authors.forename: Pierre
authors.initials: P
authors.lastname: Maud
authors.forename: Stéphane
authors.initials: S
authors.lastname: Auvin
authors.forename: Auvin
authors.initials: A
authors.lastname: Stéphane
authors.forename: Franc
authors.initials: F
authors.lastname: Colin
authors.forename: Collet
authors.initials: C
authors.lastname: Franc Cois
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gottrand
authors.forename: Gottrand
authors.initials: G
authors.lastname: Frédéric
authors.forename: Benoit
authors.initials: B
authors.lastname: Guery
authors.forename: Guery
authors.initials: G
authors.lastname: Benoit
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Husson
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology*; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C081237:LasI protein, Pseudomonas aeruginosa; D037521:Virulence Factors; D036002:ADP Ribose Transferases; C444820:exoenzyme S