title: [Protection of vascular endothelial cells from TNF-alpha induced injury by quercetin].
abstract: OBJECTIVE
  To investigate the effects of quercetin (Que) on the TNF-alpha injured human umbilical vein endothelial cell line (ECV-304).
  
  
  METHODS
  The experiments were performed in culture of TNF-alpha-induced ECV-304 in vitro. Cell viability was assessed with MTT assay. Lipid peroxidation of ECV-304 was monitored as malonaldehyde (MDA) with a fluorometric assay. NO and SOD of ECV-304 were monitored with colorimetry. The expression of NF-kappaB was detected with immunocytochemistry.
  
  
  RESULTS
  TNF-alpha inhibited the ECV-304 prolifteration. Preincubation of ECV-304 with Que for 24h before TNF-alpha exposure increased the cell viability. Que significantly decreased the level of NO and MDA and the expression of NF-kappaB, and increased the activity of SOD in TNF-alpha-induced ECV-304.
  
  
  CONCLUSIONS
  These results demonstrate that Que can produce the protective action on TNF-alpha-induced cultured ECV-304 and its mechanism of action may be related to the decrease of NO, antioxidant effect of lipids. However, antioxidant effect might be exerted through NF-kappaB activation pathway.
authors.affiliation: Medical School of Xi' an Jiaotong University, Xi'an.
authors.forename: Rong
authors.initials: R
authors.lastname: Lin
authors.forename: Juntian
authors.initials: J
authors.lastname: Liu
authors.forename: Weijie
authors.initials: W
authors.lastname: Gan
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis*; D009569:Nitric Oxide / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008315:Malondialdehyde; D011794:Quercetin; D013482:Superoxide Dismutase