title: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure.
abstract: The effects of pesticide contamination on the metabolism of marine molluscs are poorly documented. We investigated the response of a marine bivalve, the Pacific oyster, Crassostrea gigas, using a suppression subtractive hybridization method to identify up- and down-regulated genes after a 30-day exposure period to herbicides (a cocktail of atrazine, diuron and isoproturon, and to the single herbicide glyphosate). A total of 137 unique differentially expressed gene sequences was identified, as well as their associated physiological process. The expression of 18 of these genes was analyzed by RT-PCR under laboratory experimental conditions. The metabolic functions they are associated with include xenobiotic detoxification, energy production, immune system response and transcription. This study provides a preliminary basis for studying the response of marine bivalves to long-term herbicide exposure in terms of regulated gene expression and characterizes new potential genetic markers of herbicide contamination.
authors.affiliation: Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Place Nicolas Copernic, F-29280 Plouzané, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Tanguy
authors.forename: Isabelle
authors.initials: I
authors.lastname: Boutet
authors.forename: Jean
authors.initials: J
authors.lastname: Laroche
authors.forename: Dario
authors.initials: D
authors.lastname: Moraga
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D006540:Herbicides / Q000633:toxicity*; D007928:Lethal Dose 50; D010125:Ostreidae / Q000187:drug effects* / Q000378:metabolism; D020365:RNA Helicases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D006540:Herbicides; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases; D020365:RNA Helicases