Expression of lysyl oxidase isoforms in MC3T3-E1 osteoblastic cells.
Covalent intermolecular cross-linking of collagen is initiated by the action of lysyl oxidase (LOX) on the telopeptidyl lysine and hydroxylysine residues. Recently, several LOX isoforms, i.e., LOX-like proteins 1-4 (LOXL1-4), have been identified but their specific tissue distribution and functions are still largely unknown. In this study, mRNA expression of LOX and LOXL1-4 in MC3T3-E1 osteoblastic cells was screened by RT-PCR and quantitatively analyzed by real-time PCR during cell differentiation and matrix mineralization. The results demonstrated that LOX and all LOXLs, except LOXL2, were expressed in this cell line and that the expression pattern during cell differentiation and matrix mineralization was distinct from one another. This indicates that the expression of LOX and its isoforms is highly regulated during osteoblast differentiation, suggesting their distinct roles in collagen matrix stabilization and subsequent mineralization.
Atsawasuwan
Phimon
P
CB#7455, Dental Research Center, University of North Carolina at Chapel Hill, NC 27599-7455, USA.
Mochida
Yoshiyuki
Y
Parisuthiman
Duenpim
D
Yamauchi
Mitsuo
M
D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D024042:Collagen Type I; D020033:Protein Isoforms; D012333:RNA, Messenger; D008249:Protein-Lysine 6-Oxidase