title: Antioxidant properties and TLC characterization of four Chilean Haplopappus-species known as bailahuén.
abstract: "Bailahuén" is the common name of a medicinal shrub native to Chile where this resinous herb is widely used for its liver stimulating properties. Although the official species is Haplopappus baylahuen Remy (Asteraceae), other species of the same genus are also used in different regions as "bailahuén". A thin layer chromatography (TLC) method for rapid identification of different species and detection of adulterations is described for four of the species: Haplopappus baylahuen, Haplopappus taeda Reiche, Haplopappus multifolius Phil., and Haplopappus remyanus Wedd. To confirm efficiency in all species antioxidant properties were screened in resins, infusions, and methanolic extracts by tests of lipid peroxidation in erythrocytes and free radical scavenging activity by DPPH. In both studies Haplopappus baylahuen showed the lowest antioxidant capacity. In DPPH analyses, infusion and resins of Haplopappus baylahuen also showed the lowest and Haplopappus remyanus the major inhibiting activity of free radicals, while Haplopappus multifolius proved to have the highest result when the methanolic extracts were used. The chemical characterization of the studied species showed important levels of flavonoids and coumarins, with flavonoids predominating in Haplopappus taeda, coumarins in Haplopappus multifolius and both of them in Haplopappus baylahuen and Haplopappus remyanus.
authors.affiliation: Universidad de Talca, Facultad de Ciencias Agrarias, Departamento de Horticultura, Casilla 747, Talca, Chile. hvogel@utalca.cl
authors.forename: Hermine
authors.initials: H
authors.lastname: Vogel
authors.forename: Mauricio
authors.initials: M
authors.lastname: González
authors.forename: Francesca
authors.initials: F
authors.lastname: Faini
authors.forename: Iván
authors.initials: I
authors.lastname: Razmilic
authors.forename: Jaime
authors.initials: J
authors.lastname: Rodríguez
authors.forename: José San
authors.initials: JS
authors.lastname: Martín
authors.forename: Francisco
authors.initials: F
authors.lastname: Urbina
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D002677:Chile; D002855:Chromatography, Thin Layer / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D031206:Haplopappus; D006801:Humans; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D013045:Species Specificity
chemical_list: D000975:Antioxidants; D010936:Plant Extracts