Kinetochore-spindle microtubule interactions during mitosis.
The kinetochore is a proteinaceous structure that assembles onto centromeric DNA and mediates chromosome attachment to microtubules during mitosis. This description is deceivingly simple: recent proteomic studies suggest that the diminutive kinetochores of Saccharomyces cerevisiae are comprised of at least 60 proteins organized into as many as 14 different subcomplexes. Many of these proteins, such as the centromeric histone variant CENP-A, and entire subcomplexes, such as the Ndc80(Hec1) complex, are conserved from yeast to humans despite the diverse nature of the DNA sequences on which they assemble. There have recently been advances in our understanding of the molecular basis of how kinetochores establish dynamic attachments to spindle microtubules, and how these attachments are correctly oriented to ensure segregation of sister chromatids to daughter cells.
Kline-Smith
Susan L
SL
Ludwig Institute for Cancer Research, Department of Cellular & Molecular Medicine, University of California, San Diego, 9500 Gilman Dr, CMM-East, Rm 3080, La Jolla, California 92093, USA.
Sandall
Sharsti
S
Desai
Arshad
A
D000818:Animals; D002842:Chromatids / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D008938:Mitosis; D008954:Models, Biological; D009687:Nuclear Proteins / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D012854:Sister Chromatid Exchange; D008941:Spindle Apparatus
D006657:Histones; C112472:NDC80 protein, S cerevisiae; D009687:Nuclear Proteins; D011108:Polymers; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA