title: Surface modification of silicon and gold-patterned silicon surfaces for improved biocompatibility and cell patterning selectivity.
abstract: Clean silicon and gold-patterned silicon platforms were modified with methoxy-polyethylene glycol (M-PEG silane) via a self-assembly technique, which significantly improved their plasma protein resistance capability and cell patterning selectivity. Fibrinogen and IgG were used as model plasma proteins to study the efficacy of PEG layers in resisting protein adsorption. Selective cell patterning on the gold regions of a gold-patterned silicon substrate and tissue compatibility were studied with macrophage and fibroblast cells. The research also revealed how the presence of gold electrodes on a silicon substrate would influence the cell patterning selectivity. Our experimental results showed that the PEG-modified silicon surfaces had a high resistivity to protein and cell attachment and that the PEG-modified gold-patterned silicon surfaces nearly completely eliminated the protein adsorption and cell attachment on silicon. This study provides a new approach to developing biocompatible surfaces for silicon-based BioMEMS devices, particularly for biosensors where a metal-insulator format must be enforced.
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Sheeny
authors.initials: S
authors.lastname: Lan
authors.forename: Mandana
authors.initials: M
authors.lastname: Veiseh
authors.forename: Miqin
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D005340:Fibrinogen / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008422:Materials Testing; D051379:Mice; D011485:Protein Binding; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D007074:Immunoglobulin G; D012821:Silanes; D006046:Gold; D005340:Fibrinogen; D012825:Silicon