Mutagenicity testing with transgenic mice. Part I: Comparison with the mouse bone marrow micronucleus test.
As part of a larger literature study on transgenic animals in mutagenicity testing, test results from the transgenic mutagenicity assays (lacI model; commercially available as the Big Blue(R) mouse, and the lacZ model; commercially available as the Mutatrade markMouse), were compared with the results on the same substances in the more traditional mouse bone marrow micronucleus test. 39 substances were found which had been tested in the micronucleus assay and in the above transgenic mouse systems. Although, the transgenic animal mutation assay is not directly comparable with the micronucleus test, because different genetic endpoints are examined: chromosome aberration versus gene mutation, the results for the majority of substances were in agreement. Both test systems, the transgenic mouse assay and the mouse bone marrow micronucleus test, have advantages and they complement each other. However, the transgenic animal assay has some distinct advantages over the micronucleus test: it is not restricted to one target organ and detects systemic as well as local mutagenic effects.
Wahnschaffe
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U
Fraunhofer Institute of Toxicology and Experimental Medicine ITEM, Department of Chemical Risk Assessment, Nikolai-Fuchs-Str, 1, 30625 Hannover, Germany. kielhorn@item.fraunhofer.de.
Bitsch
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A
Kielhorn
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J
Mangelsdorf
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I