Separation of 2-aminobenzoic acid-derivatized glycosaminoglycans and asparagine-linked glycans by capillary electrophoresis.
A capillary electrophoresis method was developed for the analysis of oligosaccharides combined with derivatization with 2-aminobenzoic acid. Glycosaminoglycan delta-disaccharides were effectively resolved on a fused-silica capillary tube using 150 mM borate, pH 8.5, as a running electrolyte solution. This analytical method was applied to the identification of glycosaminoglycan in combination with enzymatic digestion. The separation of N-glycans or glucose-oligomers was performed with a phosphate buffer containing polyethylene glycol or borate as an electrolyte solution. This method is expected to be useful in the determination of oligosaccharide structures in a glycoprotein.
Sato
Kae
K
Department of Applied Biological Chemistry, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Sato
Kiichi
K
Okubo
Akira
A
Yamazaki
Sunao
S
D000818:Animals; D001216:Asparagine; D019765:Chondroitin ABC Lyase / Q000378:metabolism; D004187:Disaccharides / Q000032:analysis / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D006025:Glycosaminoglycans / Q000302:isolation & purification* / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification* / Q000378:metabolism; D062367:ortho-Aminobenzoates
D004187:Disaccharides; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D011134:Polysaccharides; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D001216:Asparagine; D010447:Peptide Hydrolases; C035855:keratinase; D019765:Chondroitin ABC Lyase