title: Nuclear actin is partially associated with Cajal bodies in human cells in culture and relocates to the nuclear periphery after infection of cells by adenovirus 5.
abstract: Cajal bodies are intra-nuclear structures enriched in proteins involved in transcription and mRNA processing. In this study, immunofluorescence microscopy experiments using a highly specific antibody to actin revealed nuclear actin spots that colocalized in part with p80 coilin-positive Cajal bodies. Actin remained associated with Cajal bodies in cells extracted to reveal the nuclear matrix. Adenovirus infection, which is known to disassemble Cajal bodies, resulted in loss of actin from these structures late in infection. In infected cells, nuclear actin was observed to relocate to structures at the periphery of the nucleus, inside the nuclear envelope. Based on these findings, it is suggested that actin may play an important role in the organization or function of the Cajal body.
authors.affiliation: School of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: L J E
authors.initials: LJ
authors.lastname: Gedge
authors.forename: E E
authors.initials: EE
authors.lastname: Morrison
authors.forename: G E
authors.initials: GE
authors.lastname: Blair
authors.forename: J H
authors.initials: JH
authors.lastname: Walker
mesh_terms: D000199:Actins / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000472:pathogenicity*; D036022:Capsid Proteins / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D020541:Coiled Bodies / Q000378:metabolism* / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D015530:Nuclear Matrix / Q000378:metabolism / Q000821:virology
chemical_list: D000199:Actins; D036022:Capsid Proteins; C043322:penton protein, adenovirus