Transformation of Escherichia coli mediated by natural phospholipids.
Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCl2 or CaCl2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb).
Sato
Yuko
Y
Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University, Ibaraki, Japan.
Kumazawa
Noriyuki
N
Yoshikawa
Kenichi
K
Kurusu
Yasurou
Y
D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D010714:Phosphatidylethanolamines / Q000494:pharmacology*; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine