Folding, stability and polymerization properties of FtsZ chimeras with inserted tubulin loops involved in the interaction with the cytosolic chaperonin CCT and in microtubule formation.
To attain its native conformation, the cytoskeletal protein tubulin needs the concourse of several molecular chaperones, among others the cytosolic chaperonin CCT. It has been previously described that denatured tubulin interacts with CCT in a quasi-folded conformation using several loops located throughout its sequence. These loops are also involved in microtubule formation and are absent in its prokaryote homologue FtsZ, which in vitro folds by itself and does not interact with CCT. Several FtsZ/tubulin chimeric proteins were generated by inserting consecutively one, two or three of the CCT-binding domains of tubulin into the corresponding sequence of FtsZ from Methanococccus jannaschii. The insertion of any of the CCT-binding loops generates in the FtsZ/tubulin chimeras the ability to interact with CCT. The accumulation of CCT-binding loops induces in the FtsZ/tubulin chimeras unfolding and refolding properties that are more similar to tubulin than to its prokaryote counterpart. Finally, the insertion of some of these loops generates in the FtsZ/tubulin chimeras more complex polymeric structures than those found for FtsZ. These results reinforce the notion that CCT has coevolved with tubulin to deal with the folding problems encountered by the eukaryotic protein with the appearance of the new sequences involved in microtubule formation.
Bertrand
Sara
S
Centro Nacional de Biotecnología, C.S.I.C Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Barthelemy
Isabel
I
Oliva
María A
MA
Carrascosa
José L
JL
Andreu
José M
JM
Valpuesta
José M
JM
D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000378:metabolism*; D017017:Methanococcus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012345:RNA, Transfer, Amino Acid-Specific; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D016415:Sequence Alignment; D014404:Tubulin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
D019843:Archaeal Proteins; C119586:FtsZ protein, Methanococcus jannaschii; D012345:RNA, Transfer, Amino Acid-Specific; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins