The effect of lipopolysaccharide on cholecystokinin in murine plasma and tissue.
Several mechanisms have been proposed for neuroimmune communication supporting sickness behavior (fever, anorexia, inactivity, and cachexia) following infection. We examined the role of cholecystokinin as a neurochemical intermediary of sickness behavior by determining plasma, duodenum, hypothalamus, and brainstem cholecystokinin concentrations 30 and 60 min and 12 h following intraperitoneal lipopolysaccharide (LPS) (0.25 and 2.5 mg/kg). Hypothalamic cholecystokinin was significantly lower in LPS- versus saline-treated mice 30 min (0.25 and 2.5 mg/kg) and 12 h (2.5 mg/kg) post-injection. Plasma cholecystokinin of LPS-treated mice was significantly lower than that of controls 1 and 12 h post-injection, a finding consistent with a non-endocrine action of peripheral cholecystokinin.
Weiland
Tracey J
TJ
School of Psychological Science, La Trobe University, Bundoora, Vic. 3086, Australia.
Kent
Stephen
S
Voudouris
Nicholas J
NJ
Shulkes
Arthur
A
D000818:Animals; D001921:Brain / Q000378:metabolism; D001933:Brain Stem / Q000187:drug effects; D002766:Cholecystokinin / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D013997:Time Factors
D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D002766:Cholecystokinin