Type-specific reactivity of anti-glycoprotein G antibodies from herpes simplex virus-infected individuals is maintained by single or dual type-specific residues.
Glycoprotein G-1 (gG-1) of herpes simplex virus type 1 (HSV-1) and gG-2 of HSV-2 are the only known HSV proteins that induce type-specific human antibody responses. Recently, it was shown that purified human anti-gG-1 and anti-gG-2 antibodies presented a type-specific reactivity to immunogenic stretches with high similarity between gG-1 and gG-2. In this study, the molecular basis for this type-specific recognition was investigated employing synthetic peptides covering the indicated regions, including substitutions of the type-specific residues. The results revealed that single or dual type-specific residues localized within regions of high similarity could induce significant structural differences, explaining the type-specific recognition of the human antibody response to the gG proteins.
Tunbäck
Petra
P
Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.|Department of Dermatovenereology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
Bergström
Tomas
T
Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
Löwhagen
Gun-Britt
GB
Department of Dermatovenereology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
Hoebeke
Johan
J
UPR9021 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Liljeqvist
Jan-Åke
JÅ
Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
D019943:Amino Acid Substitution; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D006561:Herpes Simplex / Q000097:blood / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000914:Antibodies, Viral; D010455:Peptides; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; C050591:glycoprotein gG-1, herpes simplex virus type 1