Comparative replication kinetics of two cytopathic feline lentiviruses ex vivo.
Feline immunodeficiency virus infection of cats provides a model to elucidate mechanisms of lentiviral pathogenesis. We isolated a non-domestic FIV from a Pallas' cat, FIV-Oma, which replicates in feline PBMCs and CRFK cells. To gain insights into FIV pathogenesis, we compared rates of viral replication and apoptosis of FIV-Oma with FIV-PPR in the MYA-1 T-cell line. To minimize heterogeneity of virus, infections were initiated with virus derived from molecular clones. Viral DNA and RNA levels, assessed by qPCR and qRT-PCR, apoptosis, and supernatant reverse transcriptase were slower in FIV-Oma infections. Immunostaining for cellular Gag showed that few cells were productively infected. The majority of cells infected with either virus instead became apoptotic. Apoptosis was detectable within 6 h PI, suggesting activation of a signaling pathway. We propose that apoptosis is due to interaction of virus with cells, and is the usual outcome of infection by cytopathic FIVs in these cells.
Sutton
Claudia A
CA
Department of Microbiology and Immunology, Veterinary Medical Center, College of Veterinary Medicine, Cornell University, C5-153 Ithaca, NY 14853, USA.
Gordnier
Pamela M
PM
Avery
Roger J
RJ
Casey
James W
JW
D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats / Q000821:virology*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D016181:Feline Acquired Immunodeficiency Syndrome / Q000473:pathology; D016089:Immunodeficiency Virus, Feline / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes; D014759:Viral Envelope Proteins / Q000737:chemistry; D014779:Virus Replication / Q000502:physiology*
D004279:DNA, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins