title: Intracellular mediators of procyanidin-induced lipolysis in 3T3-L1 adipocytes.
abstract: We have previously reported that grape seed procyanidins stimulate long-term lipolysis on 3T3-L1 fully differentiated adipocytes. To unravel the molecular mechanism by which procyanidins exert this effect, we checked the involvement of two main cellular targets in adipose cells: protein kinase A (PKA) and peroxisome proliferator-activated receptor-gamma (PPAR-gamma). Procyanidin treatment increased intracellular cAMP levels in 3T3-L1 adipocytes, and their lipolytic effect was inhibited by simultaneous treatment with H89, a PKA specific inhibitor. BRL49653, a very highly specific ligand of PPAR-gamma, totally abolished the lipolytic effect of procyanidins. Simultaneous to this long-term lipolytic effect, the mRNA levels of some differentiation adipocyte markers decreased, although there were no changes in the triglyceride content of the cells. BRL49653 did not antagonize the decrements of differentiation markers. These results support a mediation of PPAR-gamma and PKA on the lipolytic effects of procyanidins on 3T3-L1 adipocytes.
authors.affiliation: Departament de Bioquímica i Biotecnologia, CeRTA, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Montse
authors.initials: M
authors.lastname: Pinent
authors.forename: M Cinta
authors.initials: MC
authors.lastname: Bladé
authors.forename: M Josepa
authors.initials: MJ
authors.lastname: Salvadó
authors.forename: Lluís
authors.initials: L
authors.lastname: Arola
authors.forename: Anna
authors.initials: A
authors.lastname: Ardévol
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D047495:PPAR gamma / Q000819:agonists / Q000502:physiology; D044945:Proanthocyanidins / Q000494:pharmacology*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D044946:Biflavonoids; D004791:Enzyme Inhibitors; D047495:PPAR gamma; D044945:Proanthocyanidins; D045162:Thiazolidinediones; D000077154:Rosiglitazone; C017674:procyanidin; D002392:Catechin; D017868:Cyclic AMP-Dependent Protein Kinases