Cheongyeolsaseuptang inhibits production of TNF-alpha, IL-6 and IL-8 as well as NF-kappa B activation in human mast cells.
Traditional Korean medicine, Cheongyeolsaseuptang (CYSST) has been widely applied as a treatment of rheumatoid arthritis (RA) in Korea. However, its effect in experimental models remains unknown. Recent reports suggest that in patients with RA, synovial mast cells increase in number and show signs of activation and production of cytokines. In this study, we investigated the effect of CYSST on production of cytokines by activated human mast cell line, HMC-1. When CYSST (1mg/ml) was added, the production of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-8 was significantly inhibited about 37, 33.6, and 48%, respectively on phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated HMC-1 cells. In addition, CYSST inhibited PMA plus A23187-induced activation of nuclear factor-kappaB. These findings may help understanding the mechanism of action of this medicine leading to control activated mast cells on inflammatory condition like RA.
Kim
Su-Jin
SJ
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, South Korea.
Lee
Eon-Jeong
EJ
Song
Yung-Sun
YS
Jeong
Hyun-Ja
HJ
Lee
Kang-Min
KM
Kim
Hyung-Ryong
HR
Chae
Han-Jung
HJ
Shin
Tae-Yong
TY
Kim
Yun-Kyung
YK
Hong
Seung-Heon
SH
Kim
Hyung-Min
HM
D002460:Cell Line; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D007723:Korea; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D008518:Medicine, East Asian Traditional; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D028321:Plant Preparations / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000093242:Interleukin-6 Inhibitors
D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D028321:Plant Preparations; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors