An improved method for the enzymatic transformation of nucleosides into 5'-monophosphates.
An improved method to transform nucleosides into 5'-monophosphates using nucleoside phosphotransferase from Erwinia herbicola is reported. The method is based on the shift in the equilibrium state of the reaction to the formation of desired product due to its precipitation by Zn2+. Under optimal conditions, the extent of nucleoside transformations into nucleoside-5'-monophosphates were 41-91% (mol).
Barai
Vladimir N
VN
Institute of Microbiology, National Academy of Sciences, 220141 Kuprevicha 2, Minsk, Belarus.
Kvach
Sergei V
SV
Zinchenko
Anatoli I
AI
Mikhailopulo
Igor A
IA
D004789:Enzyme Activation; D004885:Erwinia / Q000201:enzymology*; D009705:Nucleosides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D010770:Phosphotransferases / Q000737:chemistry*; D012995:Solubility; D015032:Zinc / Q000737:chemistry*
D009705:Nucleosides; D010710:Phosphates; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D015032:Zinc