Recombinant viral capsids as an efficient vehicle of oligonucleotide delivery into cells.
Delivery of oligonucleotides (ON) into cells is a technical challenge. In this study, we utilized the capsid of the hepatitis B virus (HBV) to meet this goal. A single and short open reading frame of the virus programs efficient capsid production in bacteria. We show that these capsids can encapsulate ON in vitro and then mediate their delivery into cells with extreme efficiency. This process is cell type non-specific, rendering the recombinant HBV capsid a potentially valuable vehicle for ON delivery into a wide range of cultured cells.
Cooper
Arik
A
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
Shaul
Yosef
Y
D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000235:genetics*; D015202:Protein Engineering
D036022:Capsid Proteins; D009841:Oligonucleotides