Interaction of enteropathogenic Escherichia coli with human intestinal mucosa: role of effector proteins in brush border remodeling and formation of attaching and effacing lesions.
Enteropathogenic Escherichia coli (EPEC) strains deliver effector proteins Tir, EspB, Map, EspF, EspH, and EspG into host cells to induce brush border remodeling and produce attaching and effacing (A/E) lesions on small intestinal enterocytes. In this study, the role of individual EPEC effectors in brush border remodeling and A/E lesion formation was investigated with an in vitro human small intestinal organ culture model of EPEC infection and specific effector mutants. tir, map, espB, and espH mutants produced "footprint" phenotypes due to close bacterial adhesion but subsequent loss of bacteria; an espB mutant and other type III secretion system mutants induced a "noneffacing footprint" associated with intact brush border microvilli, whereas a tir mutant was able to efface microvilli resulting in an "effacing footprint"; map and espH mutants produced A/E lesions, but loss of bacteria resulted in a "pedestal footprint." An espF mutant produced typical A/E lesions without associated microvillous elongation. An espG mutant was indistinguishable from the wild type. These observations indicate that Tir, Map, EspF, and EspH effectors play a role in brush border remodeling and production of mature A/E lesions.
Shaw
Robert K
RK
Institute of Child Health, University of Birmingham, Birmingham B16 8ET, United Kingdom.
Cleary
Jennifer
J
Murphy
Michael S
MS
Frankel
Gad
G
Knutton
Stuart
S
D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D004386:Duodenum / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000378:metabolism* / Q000382:microbiology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; C429282:EspG protein, E coli; C472832:EspH protein, E coli; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli; C073819:eaeA protein, E coli