title: Functional link between ribosome formation and biogenesis of iron-sulfur proteins.
abstract: In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron-sulfur (Fe-S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe-S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe-S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in pre-rRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe-S proteins.
authors.affiliation: Biochemie-Zentrum der Universität Heidelberg (BZH), Heidelberg, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Yarunin
authors.forename: Vikram Govind
authors.initials: VG
authors.lastname: Panse
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Petfalski
authors.forename: Christophe
authors.initials: C
authors.lastname: Dez
authors.forename: David
authors.initials: D
authors.lastname: Tollervey
authors.forename: Eduard C
authors.initials: EC
authors.lastname: Hurt
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001693:Biological Transport, Active; D004271:DNA, Fungal / Q000235:genetics; D039621:Eukaryotic Initiation Factor-3 / Q000235:genetics / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D004271:DNA, Fungal; D039621:Eukaryotic Initiation Factor-3; D007506:Iron-Sulfur Proteins; C102265:NBP35 protein, S cerevisiae; C492990:RLI1 protein, S cerevisiae; D012322:RNA Precursors; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; D019204:GTP-Binding Proteins