title: Variations in the insecticide-resistance spectrum of Anopheles stephensi after selection with deltamethrin or a deltamethrin-piperonyl-butoxide combination.
abstract: When the larvae of Anopheles stephensi, a malaria vector, were selected with deltamethrin for 40 successive generations, there was a 60-fold increase in larval resistance to deltamethrin but no increase in the resistance of the adult mosquitoes. This result, and the observation that deltamethrin selection of adults for 40 generations resulted in only a six-fold increase in adult resistance to deltamethrin, indicated some stage specificity. When F(24) deltamethrin-resistant larvae were selected with 1:5 deltamethrin-piperonyl butoxide (deltamethrin-PBO), instead of deltamethrin alone, for 16 generations, the level of resistance to deltamethrin in the F(40) larvae was reduced by 6%-21%. Similarly, selection with deltamethrin-PBO of adults of the parental strain for 20 generations reduced the speed of the development of resistance to deltamethrin, compared with that seen using selection with deltamethrin alone. Deltamethrin selection appears to select initially a monooxygenase-based mechanism. When the monooxygenase-based mechanism is blocked, by treatment with PBO, selection of a kdr-type mechanism is accelerated, as is evident from increased cross-resistance to 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) in the adults selected with deltamethrin-PBO. The implications of these results are discussed in terms of the management of the larval and adult stages of An. stephensi .
authors.affiliation: Department of Zoology, University of Delhi, Delhi - 110007, India. saritasanjay@rediffmail.com
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Thomas
authors.forename: A
authors.initials: A
authors.lastname: Sahgal
authors.forename: A
authors.initials: A
authors.lastname: Verma
authors.forename: T
authors.initials: T
authors.lastname: Samuel
authors.forename: M K K
authors.initials: MK
authors.lastname: Pillai
mesh_terms: D000818:Animals; D000852:Anopheles / Q000187:drug effects* / Q000235:genetics; D003634:DDT / Q000494:pharmacology; D004199:Disease Vectors; D004338:Drug Combinations; D007305:Insecticide Resistance / Q000187:drug effects; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D008288:Malaria / Q000635:transmission; D009154:Mutation; D009570:Nitriles; D010882:Piperonyl Butoxide / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D012641:Selection, Genetic
chemical_list: D004338:Drug Combinations; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D003634:DDT; D010882:Piperonyl Butoxide