Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin fragment.
There is a synergistic effect between fibronectin and fibroblast growth factor 2 (FGF-2) on osteoblast cell adhesion through an extracellular, signal-regulated kinase pathway. Here we describe the engineering of a fusion protein containing fibronectin fragment (FNIII9-10) connected to the COOH-terminus of FGF-2. Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone (p < 0.05).
Jang
Jun-Hyeog
JH
Department of Biochemistry, Inha University College of Medicine, Jung Gu, Incheon 400-712, Korea. juhjang@inha.ac.kr
Chung
Chong-Pyoung
CP
D000327:Adsorption; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods
D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D016222:Fibroblast Growth Factor 2