Identification of insertion sequence from a gamma-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26.
Tn5-derived mutants of the gamma-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, named ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.
Miyauchi
Keisuke
K
Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan. kmiya@vos.nagaokaut.ac.jp
Fukuda
Masao
M
Tsuda
Masataka
M
Takagi
Masamichi
M
Nagata
Yuji
Y
D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D020578:Sphingomonas / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001556:Hexachlorocyclohexane