CaNAT1, a heterologous dominant selectable marker for transformation of Candida albicans and other pathogenic Candida species.
A dominant selectable marker for Candida albicans and other Candida species, which confers resistance to nourseothricin, was characterized. In a heterologous promoter system and a recyclable cassette, the marker efficiently permitted deletion and complementation of C. albicans genes. Neither growth nor filamentous development was affected in strains expressing this marker.
Shen
Junqing
J
Division of Infectious Disease, Enders 7, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA.
Guo
Weihui
W
Köhler
Julia R
JR
D000935:Antifungal Agents / Q000494:pharmacology; D001191:Arylamine N-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000472:pathogenicity; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D013309:Streptothricins / Q000494:pharmacology; D014162:Transfection
D000935:Antifungal Agents; D015415:Biomarkers; D007527:Isoenzymes; D013309:Streptothricins; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1