Leukemia inhibitory factor induces multi-lineage differentiation of adult stem-like cells in kidney via kidney-specific cadherin 16.
Side population (SP) is reported to be a stem cell-rich population. In the presence of leukemia inhibitory factor (LIF), cultured kidney SP cells differentiated into multi-lineage in collagen gel but not in synthesized polymer that has no cell adhesion factor. In cultured kidney SP cells, gene expression of kidney-specific cadherin 16 was specifically upregulated in collagen gel but not in synthesized polymer. Moreover, decreasing cadherin 16 expression using siRNA abolished LIF-induced multi-lineage differentiation of kidney SP in collagen gel. These results indicated that LIF induced multi-lineage differentiation of adult stem-like cells in kidney via cadherin 16.
Hishikawa
Keiichi
K
Department of Clinical Renal Regeneration, Graduate School of Medicine, University of Tokyo, Japan. hishikawa-tky@umin.ac.jp
Marumo
Takeshi
T
Miura
Shigeki
S
Nakanishi
Asato
A
Matsuzaki
Yumi
Y
Shibata
Katsunori
K
Kohike
Hiroko
H
Komori
Takuya
T
Hayashi
Matsuhiko
M
Nakaki
Toshio
T
Nakauchi
Hiromitsu
H
Okano
Hideyuki
H
Fujita
Toshiro
T
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019070:Cell Lineage / Q000187:drug effects / Q000502:physiology; D015850:Interleukin-6 / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000145:classification / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
D015820:Cadherins; C491946:Cdh16 protein, mouse; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse