Interleukin-6 negatively regulates Porphyromonas gingivalis fimbria-stimulated fibronectin expression in human gingival fibroblasts.
Our previous study demonstrated that fibronectin (FN) is a negative regulator of Porphyromonas gingivalis fimbria-induced pathogenesis in the initiation and development of chronic periodontal diseases. We show herein the regulatory action of interleukin-6 (IL-6) on FN expression in fimbria-treated human gingival fibroblasts. Interestingly, the decrease in FN expression in the cells treated with fimbriae at a high dose (8 microg of protein ml(-1)) was negated by treatment with anti-IL-6 antibody. Also, the increase in FN expression in cells treated with fimbriae at a low dose (1 microg of protein ml(-1)) was inhibited by exogenous IL-6. These results suggest that P. gingivalis fimbria-stimulated FN expression in human gingival fibroblasts is negatively regulated by endogenous IL-6.
Murakami
Yukio
Y
Department of Oral Diagnosis, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado City, Saitama 350-0283, Japan.
Shoji
Masao
M
Hirata
Atsushi
A
Tanaka
Shoji
S
Hanazawa
Shigemasa
S
Fujisawa
Seiichiro
S
D000328:Adult; D000368:Aged; D016866:Bacteroidaceae Infections / Q000276:immunology / Q000382:microbiology; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000382:microbiology*; D005353:Fibronectins / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D005881:Gingiva / Q000166:cytology / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000276:immunology / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000276:immunology*
D005353:Fibronectins; D015850:Interleukin-6