Interaction of N-acetylglutamate kinase with a PII-like protein in rice.
PII protein in bacteria is a sensor for 2-oxoglutarate and a transmitter for glutamine signaling. We identified an OsGlnB gene that encoded a bacterial PII-like protein in rice. Yeast two-hybrid analysis showed that an OsGlnB gene product interacted with N-acetylglutamate kinase 1 (OsNAGK1) and PII-like protein (OsGlnB) itself in rice. In cyanobacteria, NAGK is a key enzyme in arginine biosynthesis. Transient expression of OsGlnB cDNA or OsNAGK1 cDNA fused with sGFP in rice leaf blades strongly suggested that the PII-like protein as well as OsNAGK1 protein is located in chloroplasts. Both OsGlnB and OsNAGK1 genes were expressed in roots, leaf blades, leaf sheaths and spikelets of rice, and these two genes were coordinately expressed in leaf blades during the life span. Thus, PII-like protein in rice plants is potentially able to interact with OsNAGK1 protein in vivo. This finding will provide a clue to the precise physiological function of PII-like protein in rice.
Sugiyama
Kenjiro
K
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan.
Hayakawa
Toshihiko
T
Kudo
Toru
T
Ito
Takashi
T
Yamaya
Tomoyuki
T
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000378:metabolism; D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005971:Glutamates / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D051922:PII Nitrogen Regulatory Proteins; D010770:Phosphotransferases / Q000378:metabolism*; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D005971:Glutamates; D051922:PII Nitrogen Regulatory Proteins; C473074:PII protein, Arabidopsis; D010940:Plant Proteins; D010770:Phosphotransferases; D017851:Phosphotransferases (Carboxyl Group Acceptor); C018721:acetylglutamate kinase; C016195:N-acetylglutamic acid