title: NF-kappa B activation is not required for Chlamydia trachomatis inhibition of host epithelial cell apoptosis.
abstract: Chlamydia trachomatis, an obligate intracellular bacterial species, is known to inhibit host cell apoptosis. However, the chlamydial antiapoptotic mechanism is still not clear. Because NF-kappaB activation is antiapoptotic, we tested the potential role of NF-kappaB activation in chlamydial antiapoptotic activity in the current study. First, no obvious NF-kappaB activation was detected in the chlamydia-infected cells when these cells were resistant to apoptosis induced via either the intrinsic or extrinsic apoptosis pathways. Second, inhibition of NF-kappaB activation with pharmacologic reagents failed to block the chlamydial antiapoptotic activity. Finally, NF-kappaB p65 gene deletion did not prevent chlamydia from inhibiting host cell apoptosis. These observations together have demonstrated that NF-kappaB activation is not required for the chlamydial antiapoptotic activity.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
authors.forename: Yangming
authors.initials: Y
authors.lastname: Xiao
authors.forename: Youmin
authors.initials: Y
authors.lastname: Zhong
authors.forename: Heng
authors.initials: H
authors.lastname: Su
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Paul
authors.initials: P
authors.lastname: Chiao
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhong
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000187:drug effects / Q000276:immunology*; D003513:Cycloheximide / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*; D017353:Gene Deletion; D006367:HeLa Cells; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D019311:Staurosporine / Q000494:pharmacology; D051996:Transcription Factor RelA
chemical_list: D004224:Diterpenes; D016328:NF-kappa B; D012717:Sesquiterpenes; D051996:Transcription Factor RelA; C002669:parthenolide; C047382:kamebakaurin; D003513:Cycloheximide; D019311:Staurosporine