title: Single-feature inking and stamping: a versatile approach to molecular patterning.
abstract: A technique for micrometer-scale patterning of multiple functional biological molecules on surfaces is demonstrated. The technique is referred to as single-feature inking and stamping (SFINKS). It combines elements of dip-pen nanolithography and microcontact printing. "Inked" atomic force microscopy probes are used to ink individual features of an elastomer stamp. From a single stamp, we printed three different probe ssDNA with <10 mum resolution and showed that they specifically hybridize the complementary DNA labeled with different fluorophores. As a further demonstration of SFINKS' versatility, we patterned a silane onto a silicon wafer consisting of four subpatterns separated by >100 mum and composed of 2 mum lines. We discuss why patterns such as these are impractical with available techniques. Furthermore, we comment on the prospects for multiple stamping after a single inking.
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872, USA.
authors.forename: Jonathon D
authors.initials: JD
authors.lastname: Gerding
authors.forename: Dale M
authors.initials: DM
authors.lastname: Willard
authors.forename: Alan
authors.initials: A
authors.lastname: Van Orden
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D010363:Pattern Recognition, Automated / Q000379:methods*
chemical_list: D004277:DNA, Single-Stranded; D009841:Oligonucleotides