title: Ribosome bypassing at serine codons as a test of the model of selective transfer RNA charging.
abstract: Recently, a model of the flux of amino acids through transfer RNAs (tRNAs) and into protein has been developed. The model predicts that the charging level of different isoacceptors carrying the same amino acid respond very differently to variation in supply of the amino acid or of the rate of charging. It has also been shown that ribosome bypassing is specifically stimulated at 'hungry' codons calling for an aminoacyl-tRNA in short supply. We have constructed two reporters of bypassing, which differ only in the identity of the serine codon subjected to starvation. The stimulation of bypassing as a function of starvation differed greatly between the two serine codons, in good agreement with the quantitative predictions of the model.
authors.affiliation: Department of Genome Sciences, University of Washington, Box 357730, Seattle, Washington 98195-7730, USA.
authors.forename: Dale
authors.initials: D
authors.lastname: Lindsley
authors.forename: Paul
authors.initials: P
authors.lastname: Bonthuis
authors.forename: Jonathan
authors.initials: J
authors.lastname: Gallant
authors.forename: Teodora
authors.initials: T
authors.lastname: Tofoleanu
authors.forename: Johan
authors.initials: J
authors.lastname: Elf
authors.forename: Måns
authors.initials: M
authors.lastname: Ehrenberg
mesh_terms: D003062:Codon; D048168:Escherichia coli K12; D017930:Genes, Reporter; D007763:Lac Operon; D012362:RNA, Transfer, Ser / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012694:Serine / Q000235:genetics* / Q000378:metabolism
chemical_list: D003062:Codon; D012362:RNA, Transfer, Ser; D012694:Serine