title: Small fiber diameter fibro-porous meshes: tissue response sensitivity to fiber spacing.
abstract: The purpose of this research was to determine if fiber spacing for small fiber diameter fibro-porous meshes affected tissue response in vivo. Disk-shaped polyurethane meshes, with mean fiber diameters of 7.6 microm and fiber spacing between 6 and 68 microm, were implanted in rat subcutaneous dorsum for 5-week intervals and then prepared for light microscopy and morphological analysis. Results showed that implants with 12- to 68-microm spacing had no histologically apparent fibrous capsule around the perimeter, a result different from that for 6-microm spacing samples that had a capsule around a mean of 34.2% of the perimeter. For the 12- to 68-microm spacing range, a mean of 21.0% of individual fibers within the meshes were encapsulated. Qualitatively, it appeared that larger fibers were encapsulated more frequently than smaller ones. When nodeless or baggy meshes were implanted, cells tended to cluster three or more fibers into groups and then encapsulate each group. Over the 6- to 68-microm spacing range, cell nuclei volume fraction within the meshes increased from the 6- to the 29-microm spacing (p = 0.000) and then decreased from the 29- to the 68-microm spacing (p = 0.015). There was a trend of an increase in local vessel volume fraction with spacing over the 6- to 68-microm range, though the relationship was weak. The results indicate that the reason for the lack of encapsulation of small-fiber fibro-porous meshes is not exclusively a pore boundary explanation, as is proposed for small-pore porous meshes.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA. jsanders@u.washington.edu
authors.forename: J E
authors.initials: JE
authors.lastname: Sanders
authors.forename: S E
authors.initials: SE
authors.lastname: Lamont
authors.forename: S B
authors.initials: SB
authors.lastname: Mitchell
authors.forename: S G
authors.initials: SG
authors.lastname: Malcolm
mesh_terms: D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D005347:Fibroblasts / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes; D013526:Surgical Mesh
chemical_list: D001672:Biocompatible Materials; D011140:Polyurethanes