Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
The Arf tumor suppressor protein inhibits cell proliferation through both p53-dependent and -independent mechanisms. Two rat monoclonal antibodies raised against a peptide corresponding to amino acids 54-75 of the mouse p19(Arf) protein reacted with the native protein expressed in mammalian cells. Neither antibody detected human or golden hamster Arf proteins. The two antibodies, both of IgG2b isotype, are directed to adjacent epitopes contained within residues 54-62 and 62-75, respectively, of the mouse p19(Arf) protein, and both were highly efficient in detecting p19(Arf) by immunoprecipitation, immunoblotting, and immunofluorescence. One antibody proved useful for immunohistochemical staining of p19(Arf) in fixed sections of mouse testes, revealing low levels of protein expression within the nucleoli of spermatogonia. This indicates that these antibodies should be useful in detecting the endogenous p(19Arf) protein at specific stages of mouse development and during early stages of tumorigenesis.
Bertwistle
David
D
Department of Genetics and Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Zindy
Frederique
F
Sherr
Charles J
CJ
Roussel
Martine F
MF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D051379:Mice; D041681:NIH 3T3 Cells; D051381:Rats; D013045:Species Specificity; D025803:Tumor Suppressor Protein p14ARF / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; C417743:Cdkn2a protein, mouse; D019941:Cyclin-Dependent Kinase Inhibitor p16; D000939:Epitopes; D025803:Tumor Suppressor Protein p14ARF