title: Pregnancy after ICSI with ejaculated immotile spermatozoa from a patient with immotile cilia syndrome: a case report and review of the literature.
abstract: This study presents a case of intracytoplasmic sperm injection (ICSI) with ejaculated immotile spermatozoa from a patient with immotile cilia syndrome. Semen analysis of the patient suffering from immotile cilia syndrome revealed an extreme oligoasthenoteratozoospermia (OAT: count <1.4 x 10(6)/ml, 0% motility and 3% normal morphology). Electron microscopy of sperm flagella showed the absence of inner and outer dynein arms. During the ICSI cycle, the hypo-osmotic swelling test (HOS) was used for the identification of viable spermatozoa in the pool of immotile spermatozoa for ICSI. A normal fertilization rate was found in eight out of the 12 oocytes. A first fresh double embryo transfer resulted in a late miscarriage at 21 weeks. A second healthy singleton pregnancy occurred after transfer of two frozen-thawed embryos from the same ICSI procedure. Although only one successful ICSI case of the immotile cilia syndrome combined with HOS is described here, HOS might be a simple but valuable tool to obtain normal fertilization and pregnancy for patients suffering from immotile spermatozoa.
authors.affiliation: Genk Institute for Fertility Technology, Department of Obstetrics and Gynaecology, Ziekenhuis Oost-Limburg, Schiepse Bos 6, 3600 Genk, Belgium.
authors.forename: Karen
authors.initials: K
authors.lastname: Peeraer
authors.forename: Martine
authors.initials: M
authors.lastname: Nijs
authors.forename: Dominique
authors.initials: D
authors.lastname: Raick
authors.forename: Willem
authors.initials: W
authors.lastname: Ombelet
mesh_terms: D000328:Adult; D002925:Ciliary Motility Disorders / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D004542:Ejaculation; D005260:Female; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D009995:Osmosis; D011247:Pregnancy; D012720:Severity of Illness Index; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure