title: Interaction between trehalose and alkaline-earth metal ions.
abstract: We investigated the interaction between trehalose and alkaline-earth metal ions. The nuclear relaxation times of carbon atoms of trehalose were shortened by addition of the alkaline-earth chloride salts, MgCl2, CaCl2, and SrCl2, indicating that trehalose formed metal-complexes with the alkaline-earth metal chlorides. From the data of the 1H-1H coupling constants of trehalose in the presence of the alkaline-earth chlorides, it appeared that trehalose formed complexes with MgCl2, and CaCl2 at the various complexing sites: Mg2+ was coordinated to O-4 and O-4' of trehalose, and Ca2+ to O-2 and O-3. We succeeded in the preparation of two types of crystals of the trehalose/CaCl2. One was a crystal consisting of trehalose, CaCl2, and water in a ratio of 1:1:1. The other was an anhydrous crystal containing trehalose and CaCl2 in a ratio of 1:2. Several applications of the complexing between trehalose and the metal ions for food processing are proposed.
authors.affiliation: Amase Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan. okukaz@hayashibara.co.jp
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Oku
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kurose
authors.forename: Michio
authors.initials: M
authors.lastname: Kubota
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Kurimoto
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Tujisaka
authors.forename: Minoru
authors.initials: M
authors.lastname: Sakurai
mesh_terms: D000818:Animals; D002122:Calcium Chloride / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D003460:Crystallization; D015636:Magnesium Chloride / Q000737:chemistry*; D008460:Meat; D008968:Molecular Conformation; D010710:Phosphates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D018724:Spinacia oleracea / Q000737:chemistry; D013324:Strontium / Q000737:chemistry*; D013552:Swine; D014199:Trehalose / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D010710:Phosphates; D017680:Potassium Compounds; D015636:Magnesium Chloride; D002734:Chlorophyll; C013216:potassium phosphate; D014199:Trehalose; C025700:strontium chloride; D002122:Calcium Chloride; D013324:Strontium