Structure of the armadillo repeat domain of plakophilin 1.
The p120ctn subfamily of armadillo domain proteins has roles in modulating intercellular adhesion by cadherin-containing junctions. We have determined the crystal structure of the arm repeat domain from plakophilin-1 (PKP1), a member of the p120ctn subfamily that is found in desmosomes. The structure reveals that the domain has nine instead of the expected ten arm repeats. A sequence predicted to be an arm repeat is instead a large insert which serves as a wedge that produces a significant bend in the overall domain structure. Structure-based sequence alignments indicate that the nine repeats and large insert are common to this subfamily of armadillo proteins. A prominent basic patch on the surface of the protein may serve as a binding site for partners of these proteins.
Choi
Hee-Jung
HJ
Department of Structural Biology, Stanford University School of Medicine, 299 Campus Drive West, Stanford, CA 94305-5126, USA.
Weis
William I
WI
D000595:Amino Acid Sequence; D051186:Armadillo Domain Proteins; D051177:Catenins; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D003896:Desmosomes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism; D051191:Plakophilins; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D016415:Sequence Alignment; D055672:Static Electricity; D040681:Structural Homology, Protein; D015534:Trans-Activators / Q000737:chemistry* / Q000378:metabolism*; D000095002:Delta Catenin
D051186:Armadillo Domain Proteins; D051177:Catenins; D015815:Cell Adhesion Molecules; D008024:Ligands; C495335:PKP1 protein, human; D010750:Phosphoproteins; D051191:Plakophilins; D011506:Proteins; D015534:Trans-Activators; D000095002:Delta Catenin; C000722347:CTNND1 protein, human