SARS molecular detection external quality assurance.
Inactivated severe acute respiratory syndrome-associated coronavirus samples were used for an external quality assurance study within the World Health Organization SARS Reference and Verification Network and other reference institutions. Of 58 participants, 51 correctly detected virus in all samples > or =9,400 RNA copies per milliliter and none in negative samples. Commercial test kits significantly improved the outcome.
Drosten
Christian
C
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Doerr
Hans Wilhelm
HW
Lim
Wilina
W
Stöhr
Klaus
K
Niedrig
Matthias
M
D006801:Humans; D007753:Laboratories / Q000592:standards; D011786:Quality Control; D012367:RNA, Viral / Q000032:analysis*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*
D012367:RNA, Viral