Protective properties of melatonin-loaded nanoparticles against lipid peroxidation.
The aim of this study was to prepare melatonin-loaded nanoparticles (nanocapsules and nanospheres) by nanoprecipitation, using Eudragit S100 as polymer. The potential of these systems to protect lipids against peroxidation was evaluated in comparison to melatonin in aqueous solution and nanoemulsion. Liposomes and microsomes were used as model of a lipid membrane and lipid peroxidation was induced by free radical ascorbyl. Nanocapsule and nanosphere suspensions presented total recoveries of melatonin near 100% and associated drug around 55%. The zeta potential values were negative and the hydrodynamic diameter of particles were lower than 255 nm. The results demonstrate that the lipids were protected against peroxidation from 8 to 51% due to the presence of the melatonin and that this effect depended on the drug dose, the type of the lipid substrate and the type of colloid, in which melatonin was incorporated. Nanocapsules and nanospheres provided an important increase in the antioxidant effect of melatonin against lipid peroxidation.
Schaffazick
S R
SR
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil.
Pohlmann
A R
AR
de Cordova
C A S
CA
Creczynski-Pasa
T B
TB
Guterres
S S
SS
D004304:Dosage Forms; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008550:Melatonin / Q000494:pharmacology*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods / Q000639:trends; D020011:Protective Agents / Q000494:pharmacology*
D004304:Dosage Forms; D020011:Protective Agents; D008550:Melatonin