Sequence of a novel HLA-A*0301 intronic variant (A*03010103).
We report here the full-length sequence of a novel HLA-A*0301 allele, A*03010103, which differs from A*03010101 by a single nucleotide substitution (G>T) at position 492 within intron 2. The variant was originally identified by Reference Strand-mediated Conformational Analysis (RSCA) and was confirmed by cloning and sequencing. The difference in RSCA mobility between A*03010101 and A*03010103 demonstrates the sensitivity of RSCA to detect single nucleotide polymorphisms.
Mayor
N P
NP
The Anthony Nolan Research Institute, The Royal Free Hospital, Hampstead, London, UK.
Cox
S T
ST
McWhinnie
A J
AJ
Argüello
J R
JR
Shaw
B E
BE
Little
A-M
AM
Madrigal
J A
JA
Marsh
S G E
SG
D001483:Base Sequence; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D015791:HLA-A3 Antigen; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data
D015234:HLA-A Antigens; C442141:HLA-A*03:01 antigen; D015791:HLA-A3 Antigen