Crystal structure of the complex formed between a group I phospholipase A2 and a naturally occurring fatty acid at 2.7 A resolution.
This is the first evidence of a naturally bound fatty acid to a group I Phospholipase A(2) (PLA(2)) and also to a PLA(2) with Asp 49. The fatty acid identified as n-tridecanoic acid is observed at the substrate recognition site of PLA(2) hydrophobic channel. The complex was isolated from the venom of Bungarus caeruleus (Common Indian Krait). The primary sequence of the PLA(2) was determined using the cDNA method. Three-dimensional structure has been solved by the molecular replacement method and refined using the CNS package to a final R factor of 19.8% for the data in the resolution range from 20.0 to 2.7 A. The final refined model is comprised of 912 protein atoms, one sodium ion, one molecule of n-tridecanoic acid, and 60 water molecules. The sodium ion is located in the calcium-binding loop with a sevenfold coordination. A characteristic extra electron density was observed in the hydrophobic channel of the enzyme, into which a molecule of n-tridecanoic acid was clearly fitted. The MALDI-TOF measurements of the crystals had earlier indicated an increase in the molecular mass of PLA(2) by 212 Da over the native PLA(2). A major part of the ligand fits well in the binding pocket and interacts directly with His 48 and Asp 49. Although the overall structure of PLA(2) in the present complex is similar to the native structure reported earlier, it differs significantly in the folding of its calcium-binding loop.
Singh
Garima
G
Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India. tps@aiims.aiims.ac.in.
Jasti
Jayasankar
J
Saravanan
K
K
Sharma
Sujata
S
Kaur
Punit
P
Srinivasan
A
A
Singh
Tej P
TP
D000473:Alkanes / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D017838:Bungarus / Q000378:metabolism; D002118:Calcium / Q000737:chemistry; D018360:Crystallography, X-Ray; D018076:DNA, Complementary / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000737:chemistry; D004583:Electrons; D005227:Fatty Acids / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012910:Snake Venoms; D012964:Sodium / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000473:Alkanes; D018076:DNA, Complementary; D003998:Dicarboxylic Acids; D005227:Fatty Acids; D012910:Snake Venoms; D001224:Aspartic Acid; D006639:Histidine; C094073:tridecanedioic acid; D012964:Sodium; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium