title: Ribosomal protein L1 recognizes the same specific structural motif in its target sites on the autoregulatory mRNA and 23S rRNA.
abstract: The RNA-binding ability of ribosomal protein L1 is of profound interest since the protein has a dual function as a ribosomal protein binding rRNA and as a translational repressor binding its mRNA. Here, we report the crystal structure of ribosomal protein L1 in complex with a specific fragment of its mRNA and compare it with the structure of L1 in complex with a specific fragment of 23S rRNA determined earlier. In both complexes, a strongly conserved RNA structural motif is involved in L1 binding through a conserved network of RNA-protein H-bonds inaccessible to the solvent. These interactions should be responsible for specific recognition between the protein and RNA. A large number of additional non-conserved RNA-protein H-bonds stabilizes both complexes. The added contribution of these non-conserved H-bonds makes the ribosomal complex much more stable than the regulatory one.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences 142290 Pushchino, Moscow region, Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Nevskaya
authors.forename: Svetlana
authors.initials: S
authors.lastname: Tishchenko
authors.forename: Azat
authors.initials: A
authors.lastname: Gabdoulkhakov
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Nikonova
authors.forename: Oleg
authors.initials: O
authors.lastname: Nikonov
authors.forename: Alexei
authors.initials: A
authors.lastname: Nikulin
authors.forename: Olga
authors.initials: O
authors.lastname: Platonova
authors.forename: Maria
authors.initials: M
authors.lastname: Garber
authors.forename: Stanislav
authors.initials: S
authors.lastname: Nikonov
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Piendl
mesh_terms: D001665:Binding Sites; D006706:Homeostasis; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D019642:RNA, Archaeal / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000737:chemistry* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D019642:RNA, Archaeal; D012333:RNA, Messenger; D012338:RNA, Ribosomal, 23S; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; C028546:ribosomal protein L1