title: Novel method for the authentication of frigate tunas (Auxis thazard and Auxis rochei) in commercial canned products.
abstract: A novel procedure for the authentication of frigate tunas (Auxis thazard and Auxis rochei) in commercially canned products has been developed. Three mitochondrial regions were simultaneously amplified by multiplex-Polymerase Chain Reaction, one corresponding to the small rRNA 12S subunit as a positive amplification control and two species-specific fragments corresponding to cytochrome b for A. rochei and ATPase 6 for A. thazard, respectively. Testing of two different detection systems revealed the fluorescence-based approach as the most sensitive. The results demonstrate that this rapid, low-cost methodology is a reliable molecular tool for direct application in the authentication of canned products.
authors.affiliation: Laboratorio de Identificación de Especies Pesqueras y Acuícolas, CIFPA El Toruño, IFAPA Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, 11500 El Puerto de Santa María (Cádiz), Spain. carlos.infante.ext@juntadeandalucia.es
authors.forename: Carlos
authors.initials: C
authors.lastname: Infante
authors.forename: Gaetano
authors.initials: G
authors.lastname: Catanese
authors.forename: Marian
authors.initials: M
authors.lastname: Ponce
authors.forename: Manuel
authors.initials: M
authors.lastname: Manchado
mesh_terms: D000818:Animals; D001483:Base Sequence; D005396:Fish Products / Q000032:analysis; D005519:Food Preservation / Q000592:standards*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000032:analysis; D014413:Tuna / Q000235:genetics*
chemical_list: D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S