Involvement of the S100B in cAMP-induced cytoskeleton remodeling in astrocytes: a study using TRTK-12 in digitonin-permeabilized cells.
1. Stellation of astrocytes in culture involves a complex rearrangement of microfilaments, intermediate filaments, and microtubules, which reflects in part the plasticity of these cells observed during development or after injury. 2. An astrocytic calcium-binding protein, S100B, has been implicated in the regulation of plasticity due to its ability to interact with cytoskeletal proteins. 3. We used digitonin-permeabilized astrocytes to introduce TRTK-12, a peptide that binds to the C-terminal of S100B and blocks its interaction with cytoskeletal proteins. 4. TRTK-12 was able to block cAMP-induced astrocyte stellation and this effect was dependent on the concentration of the peptide. These results support the idea that S100B has a modulatory role on astrocyte morphology.
Frizzo
Juliana K
JK
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Tramontina
Ana Carolina
AC
Tramontina
Francine
F
Gottfried
Carmem
C
Leal
Rodrigo B
RB
Donato
Rosario
R
Gonçalves
Carlos-Alberto
CA
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D051347:CapZ Actin Capping Protein; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D004072:Digitonin / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009414:Nerve Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology; D010446:Peptide Fragments; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism* / Q000494:pharmacology
D051347:CapZ Actin Capping Protein; D009414:Nerve Growth Factors; D009842:Oligopeptides; D010446:Peptide Fragments; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat; C475482:TRTK-12 peptide; D000242:Cyclic AMP; D004072:Digitonin