The polymerase (L) protein of rinderpest virus interacts with the host cell protein striatin.
Rinderpest virus (RPV) is a morbillivirus that causes a highly contagious disease affecting members of the order Artiodactyla. The viral L protein is the catalytic subunit of the RNA-dependent RNA polymerase. To search for host cell proteins with which L interacts, a library screen was performed using the yeast two-hybrid system. Several host cell proteins were recovered from the library screen as putative L-interactors; one of these was identified as striatin. A direct interaction between RPV L and striatin was confirmed using both co-immunoprecipitation assays and co-localisation studies using confocal microscopy. Striatin was also shown to co-localise with the RPV L protein in infected cells. The L proteins of morbilliviruses consist of three long highly conserved domains separated by short unconserved stretches of amino acids. The L domain with which striatin interacts was investigated by co-immunoprecipitation and striatin was shown to interact primarily with the central conserved domain.
Sleeman
Katrina
K
Molecular Virology and Parasitology, Institute for Animal Health, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Baron
Michael D
MD
D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D017124:Conserved Sequence; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012302:Rinderpest virus / Q000201:enzymology*; D014407:Tumor Cells, Cultured; D020798:Two-Hybrid System Techniques
D002148:Calmodulin-Binding Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012367:RNA, Viral; C491925:STRN protein, human; D012324:RNA-Dependent RNA Polymerase