title: Efficient secretion of human lysozyme from the yeast, Kluyveromyces lactis.
abstract: Efficient secretion of human lysozyme from the yeast, Kluyveromyces lactis, was achieved by using more stable vectors in the order of S11 replication origin-containing episomal vector < full-length K. lactis plasmid pKD1-containing vector < centromeric vector < chromosome-integrated vectors. Cells containing a PGK (phosphoglycerate kinase) promoter-driven integration vector grown in non-selective rich medium achieved the highest level of secretion, approximately 100 microg lysozyme secretion ml(-1) culture: this level was approximately 10-fold higher than that achieved by episomal vectors. An additional copy of the protein disulfide isomerase gene further facilitated the secretion.
authors.affiliation: Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Iwata
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Tanaka
authors.forename: Masae
authors.initials: M
authors.lastname: Suetsugu
authors.forename: Matsujiro
authors.initials: M
authors.lastname: Ishibashi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tokunaga
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Masao
authors.initials: M
authors.lastname: Tokunaga
mesh_terms: D024861:Genetic Enhancement / Q000379:methods; D006801:Humans; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics*; D009113:Muramidase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D014162:Transfection / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; D009113:Muramidase