title: VCAM-1 expression on CD8+ cells correlates with enhanced anti-HIV suppressing activity.
abstract: CD8(+) cells from HIV-infected individuals showing the CD8(+) cell noncytotoxic antiviral response unexpectedly revealed mRNA for VCAM-1, a cell surface molecule found on endothelial cells. Uninfected subjects had undetectable levels of VCAM-1 mRNA in their CD8(+) cells. Flow cytometry analysis showed that up to 12% of the CD8(+) cells from HIV-positive individuals expressed VCAM-1 compared with 0.8% of the CD8(+) cells of HIV-negative individuals. Enrichment of the CD8(+)VCAM-1(+) cell population and subsequent coculture with CD4(+) cells acutely infected with HIV-1 showed that the VCAM-1(+)CD8(+) cells were able to suppress viral replication with 50% less input cells than the unseparated CD8(+) cell population. This study demonstrates, for the first time, the expression of VCAM-1 on CD8(+) cells. Moreover, the CD8(+)VCAM-1(+) cells show enhanced CD8(+) cell noncytotoxic antiviral response activity that could have clinical importance in HIV infection.
authors.affiliation: Department of Medicine, Division of Hematology/Oncology, University of California, San Francisco, CA 94143, USA.
authors.forename: Leyla S
authors.initials: LS
authors.lastname: Diaz
authors.forename: Hillary
authors.initials: H
authors.lastname: Foster
authors.forename: Mars R
authors.initials: MR
authors.lastname: Stone
authors.forename: Sue
authors.initials: S
authors.lastname: Fujimura
authors.forename: David A
authors.initials: DA
authors.lastname: Relman
authors.forename: Jay A
authors.initials: JA
authors.lastname: Levy
mesh_terms: D000208:Acute Disease; D000276:Adjuvants, Immunologic / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D019380:Anti-HIV Agents / Q000378:metabolism*; D000998:Antiviral Agents / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism / Q000821:virology; D002478:Cells, Cultured; D018920:Coculture Techniques; D018023:HIV Seronegativity / Q000276:immunology; D006679:HIV Seropositivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015497:HIV-1 / Q000254:growth & development / Q000276:immunology; D006801:Humans; D019409:Interleukin-15 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism / Q000821:virology; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
chemical_list: D000276:Adjuvants, Immunologic; D019380:Anti-HIV Agents; D000998:Antiviral Agents; D019409:Interleukin-15; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1