Protein disulfide isomerase immunoreactivity and protein level changes in neurons and astrocytes in the gerbil hippocampal CA1 region following transient ischemia.
We investigated the temporal and spatial alterations of protein disulfide isomerase (PDI) immunoreactivity and protein level in the hippocampus proper after 5 min transient forebrain ischemia in gerbils. PDI immunoreactivity was significantly altered in the hippocampal CA1 region. PDI immunoreactivity in the sham-operated animals was found in non-pyramidal cells. At 30 min after ischemia, PDI immunoreactivity was shown in the pyramidal cells of the stratum pyramidale (SP): the PDI immunoreactivity in the pyramidal cells was increased up to 12 h after ischemia. Thereafter PDI immunoreactivity was decreased, and the PDI immunoreactivity was shown in non-pyramidal cells 2 days after ischemia. Four to 5 days after ischemia, almost pyramidal cells in the CA1 region were lost because the delayed neuronal death occurred. At this time period, PDI immunoreactivity was expressed in some astrocytes as well as some neurons. The results of the Western blot analysis were consistent with the immunohistochemical data. These findings suggest that increase of PDI in pyramidal cells may play a critical role in resistance to ischemic damage at early time after ischemic insult, and that expression of this protein in astrocytes at late time after ischemic insult is partly implicated in the acquisition of tolerance against ischemic stress.
Hwang
In Koo
IK
Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
Yoo
Ki-Yeon
KY
Kim
Dae Won
DW
Han
Byoung Hee
BH
Kang
Tae-Cheon
TC
Choi
Soo Young
SY
Kim
Jong Sung
JS
Won
Moo Ho
MH
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001253:Astrocytes / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D005849:Gerbillinae; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism / Q000473:pathology; D011930:Reaction Time / Q000502:physiology; D013312:Stress, Physiological / Q000378:metabolism / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology
D005904:Glial Fibrillary Acidic Protein; D019704:Protein Disulfide-Isomerases