title: Inhibition of 6A8 alpha-mannosidase gene expression resulted in telomere length shortening in nasopharyngeal carcinoma cell CNE-2L2.
abstract: Telomere length shortening was observed in the nasopharyngeal carcinoma cell CNE-2L2 when 6A8 alpha-mannosidase expression was inhibited by antisense 6A8 DNA. Transduction with mock or an irrelevant DNA did not affect the telomere length in the carcinoma cells. Telomerase activity and mRNA transcription of TRF 1 and 2 were not changed in the cells treated with antisense 6A8. The Con A binding test showed an enhancement on the proteins isolated from the cells treated with antisense 6A8, but not on those from mock- or irrelevant DNA-treated cells. The data imply an association between glycosylation modification with telomere shortening in antisense 6A8-treated cells.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Jin
authors.forename: W
authors.initials: W
authors.lastname: Yue
authors.forename: G X
authors.initials: GX
authors.lastname: Shi
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: F T
authors.initials: FT
authors.lastname: Zhao
authors.forename: L P
authors.initials: LP
authors.lastname: Zhu
mesh_terms: D045744:Cell Line, Tumor; D003208:Concanavalin A / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics; D035182:TATA Box Binding Protein-Like Proteins / Q000235:genetics; D016615:Telomere; D035321:Telomeric Repeat Binding Protein 1 / Q000235:genetics; D035341:Telomeric Repeat Binding Protein 2; D043323:alpha-Mannosidase / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D009687:Nuclear Proteins; D035182:TATA Box Binding Protein-Like Proteins; C504753:TERF2 protein, human; D035321:Telomeric Repeat Binding Protein 1; D035341:Telomeric Repeat Binding Protein 2; D003208:Concanavalin A; D043323:alpha-Mannosidase