title: Long-term potentiation of synaptic transmission at the mossy fiber-granule cell relay of cerebellum.
abstract: In the last decade, the physiology of cerebellar neurons and synapses has been extended to a considerable extent. We have found that the mossy fiber-granule cell relay can generate a complex form of long-term potentiation (mf-GrC LTP) following high-frequency mf discharge. Induction. Mf-GrC LTP depends on NMDA and mGlu receptor activation, intracellular Ca(2+) increase, PKC activation, and NO production. The preventative action of intracellular agents (BAPTA, PKC-inhibitors) and of membrane hyperpolarization, and the correlated increase in intracellular Ca(2+) observed using fluorescent dyes, indicate that induction occurs postsynaptically. Expression. Expression includes three components: (a) an increase of synaptic currents, (b) an increase of intrinsic excitability in GrC, and (c) an increase of intrinsic excitability in mf terminals. Based on quantal analysis, the EPSC increase is mostly explained by enhanced neurotransmitter release. NO is a candidate retrograde neurotransmitter which could determine both presynaptic current changes and LTP. NO cascade blockers inhibit both presynaptic current changes and LTP. The increase in intrinsic excitability involves a raise in apparent input resistance in the subthreshold region and a spike threshold reduction. Together with other forms of cerebellar plasticity, mf-GrC LTP opens new hypothesis on how the cerebellum processes incoming information.
authors.affiliation: Department of Cellular and Molecular Physiology and Pharmacology, University Pavia and INFM, Via Forlanini 6, I-27100 Pavia, Italy. dangelo@unipv.it
authors.forename: Egidio
authors.initials: E
authors.lastname: D'Angelo
authors.forename: Paola
authors.initials: P
authors.lastname: Rossi
authors.forename: David
authors.initials: D
authors.lastname: Gall
authors.forename: Francesca
authors.initials: F
authors.lastname: Prestori
authors.forename: Thierry
authors.initials: T
authors.lastname: Nieus
authors.forename: Arianna
authors.initials: A
authors.lastname: Maffei
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Sola
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D017774:Long-Term Potentiation / Q000502:physiology*; D009412:Nerve Fibers / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*