VCAM-1 expression on CD8+ cells correlates with enhanced anti-HIV suppressing activity.
CD8(+) cells from HIV-infected individuals showing the CD8(+) cell noncytotoxic antiviral response unexpectedly revealed mRNA for VCAM-1, a cell surface molecule found on endothelial cells. Uninfected subjects had undetectable levels of VCAM-1 mRNA in their CD8(+) cells. Flow cytometry analysis showed that up to 12% of the CD8(+) cells from HIV-positive individuals expressed VCAM-1 compared with 0.8% of the CD8(+) cells of HIV-negative individuals. Enrichment of the CD8(+)VCAM-1(+) cell population and subsequent coculture with CD4(+) cells acutely infected with HIV-1 showed that the VCAM-1(+)CD8(+) cells were able to suppress viral replication with 50% less input cells than the unseparated CD8(+) cell population. This study demonstrates, for the first time, the expression of VCAM-1 on CD8(+) cells. Moreover, the CD8(+)VCAM-1(+) cells show enhanced CD8(+) cell noncytotoxic antiviral response activity that could have clinical importance in HIV infection.
Diaz
Leyla S
LS
Department of Medicine, Division of Hematology/Oncology, University of California, San Francisco, CA 94143, USA.
Foster
Hillary
H
Stone
Mars R
MR
Fujimura
Sue
S
Relman
David A
DA
Levy
Jay A
JA
D000208:Acute Disease; D000276:Adjuvants, Immunologic / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D019380:Anti-HIV Agents / Q000378:metabolism*; D000998:Antiviral Agents / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism / Q000821:virology; D002478:Cells, Cultured; D018920:Coculture Techniques; D018023:HIV Seronegativity / Q000276:immunology; D006679:HIV Seropositivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015497:HIV-1 / Q000254:growth & development / Q000276:immunology; D006801:Humans; D019409:Interleukin-15 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism / Q000821:virology; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
D000276:Adjuvants, Immunologic; D019380:Anti-HIV Agents; D000998:Antiviral Agents; D019409:Interleukin-15; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1