Tiam1 gene expression and its significance in colorectal carcinoma.
OBJECTIVE
To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis.


METHODS
Expressions of Tiam1 gene in 8 colorectal carcinoma cell lines were detected by reverse transcriptase-polymerase chain reaction. In vitro invasiveness was determined by means of Matrigel invasion assay. The correlation of Tiam1 expression with the invasive ability was also analyzed.


RESULTS
Tiam1 gene was highly expressed in LoVo and SW620, which were established from metastatic colorectal carcinomas in comparison with LS174T, SW480, HCT116, LST, HRT-18 and Hee8693, which were established from primary colorectal carcinomas. In vitro cell invasion demonstrated that LoVo and SW620 had a higher invasive ability than LS174T, SW480, HCT116, LST, HRT-18 and Hee8693. The expression of Tiam1 gene was highly related to the metastatic potential of colorectal carcinoma cells.


CONCLUSIONS
Tiam1 gene may play an important role in invasion and metastasis of colorectal carcinoma and is a metastasis-related gene.
Liu
Li
L
Department of Pathology, First Military Medical University, Guangzhou 510515, Guangdong Province, China.
Wu
De-Hua
DH
Ding
Yan-Qing
YQ
D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000556:secondary*; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D009361:Neoplasm Invasiveness; D011506:Proteins / Q000235:genetics*; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1
D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C087334:TIAM1 protein, human