Developmental profiles of 5-aminolevulinate, porphobilinogen and porphobilinogen synthase activity in Pieris brassicae related to the synthesis of the bilin-binding protein.
The bilin-binding protein (BBP) occurs as a major soluble protein in haemolymph, fat body, epidermis and wings of Pieris brassicae. It is a member of the lipocalin protein superfamily with yet unknown function. Here, we studied the developmental regulation of tetrapyrrole biosynthesis that provides the bilin ligand as the predominating end product. The levels of the precursors 5-aminolevulinate (ALA) and porphobilinogen (PBG) varied during larval-pupal transition in accordance with the activity of the related enzyme porphobilinogen synthase (PBGS). During adult development, both precursors were low while PBGS activity increased parallel to the formation of BBP, as shown in previous work. A competitive inhibitor of PBGS was partially purified from the meconium and characterised as a heat-stabile acidic compound. Label from [14C]ALA, injected into developing pupae of different age, was found to 80% in the hind wings and to 20% in the forewings after adult eclosion, reflecting the unequal distribution of BBP between the pairs of wings. This contrasted to the activity of PBGS that was equally active in forewings and hind wings. Together with the variation of enzyme activity during wing development our results led us propose that the (hind) wings may play a role in the synthesis of the tetrapyrrole ligand of BBP.
Kayser
Hartmut
H
Abteilung für Biologie I, Universität Ulm, Albert-Einstein-Allee 11, D-89069 Ulm, Germany. hartmut.kayser@syngenta.com
Krull-Savage
Ute
U
Rilk-van Gessel
Roland
R
D000096:Acetone / Q000031:analogs & derivatives* / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism*; D000818:Animals; D002080:Butterflies / Q000254:growth & development / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000096:biosynthesis*; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D011162:Porphobilinogen / Q000378:metabolism*; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D013997:Time Factors; D014921:Wings, Animal / Q000378:metabolism
D002352:Carrier Proteins; D019476:Insect Proteins; C053849:bilin-binding protein, insect; D000096:Acetone; D011162:Porphobilinogen; D000622:Aminolevulinic Acid; D000623:Porphobilinogen Synthase; C006495:aminoacetone