title: Semi-nested PCR detection of Bartonella henselae in Ixodes persulcatus ticks from Western Siberia, Russia.
abstract: Questing adult Ixodes persulcatus ticks from Western Siberia, Russia were tested for infections with Bartonella spp. using seminested PCR assay with primers specific to the groEL gene. The proportion of ticks infected with Bartonella spp. was 44% in 2002 (n = 50) and 38% in 2003 (n = 50). Nucleotide sequences of a portion of the PCR products corresponded to Bartonella henselae species.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia. mov@niboch.nsc.ru
authors.forename: O V
authors.initials: OV
authors.lastname: Morozova
authors.forename: F C
authors.initials: FC
authors.lastname: Cabello
authors.forename: A K
authors.initials: AK
authors.lastname: Dobrotvorsky
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001474:Bartonella Infections / Q000635:transmission; D018416:Bartonella henselae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D005260:Female; D018884:Ixodes / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D012800:Siberia; D013045:Species Specificity
chemical_list: D018834:Chaperonin 60; D004269:DNA, Bacterial