title: Antibody response to collagen after functional implantation of different polyester vascular prostheses in pigs.
abstract: Besides inflammation, specific immune responses are seen also after implantation of biomaterials. The aim was to investigate the humoral response to bovine collagen type I following implantation of various polyester (Dacron) prostheses into pigs. In 24 randomized pigs, the infrarenal aorta was replaced with a segment of collagen-impregnated, woven polyester prosthesis of low, medium, or high porosity. IgG antibodies were detected by immunoassay using native and denatured collagen type I as a target for blood samples taken on day 1 (implantation), 10, 17, 24, 62, and 116. As generally observed, antibodies to native and denatured collagen are of low titer and were significantly correlated with enhanced binding to the denatured form (p < 0.001). The highest overall antibody prevalence to native and denatured collagen was obtained on day 116 with 68% and on day 62 with 59%, respectively. Prostheses with high porosity induced an early immune response on day 10; those with low and medium porosity induced the highest antibody levels later after 2 months. Collagen antibodies neither correlated with serum IgG contents nor with antibodies to the prosthesis polyester matrix. Thus, humoral immune response against implant components may provide a further parameter in describing biocompatibility but also a potential marker that may facilitate monitoring of individual perigraft reaction.
authors.affiliation: Institute of Pathophysiology of the Ernst Moritz Arndt University Greifswald, Karlsburg D-17495, Germany. schlosse@uni-greifswald.de
authors.forename: M
authors.initials: M
authors.lastname: Schlosser
authors.forename: R
authors.initials: R
authors.lastname: Zippel
authors.forename: A
authors.initials: A
authors.lastname: Hoene
authors.forename: G
authors.initials: G
authors.lastname: Urban
authors.forename: T
authors.initials: T
authors.lastname: Ueberrueck
authors.forename: F
authors.initials: F
authors.lastname: Marusch
authors.forename: A
authors.initials: A
authors.lastname: Koch
authors.forename: L
authors.initials: L
authors.lastname: Meyer
authors.forename: L
authors.initials: L
authors.lastname: Wilhelm
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D002417:Cattle; D003094:Collagen / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D011091:Polyesters; D013552:Swine; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D007074:Immunoglobulin G; D011091:Polyesters; D003094:Collagen