Metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 and its mutant.
The metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 from Bacillus megaterium and a mutant enzyme of it (AL4V; Ala74Gly, Phe87Val, Leu188Gln triple mutant) was examined. Both purified enzymes metabolized 1-monochloro-, 2,3-dichloro-, and 2,3,7-trichloro-dibenzo-p-dioxin, but not 2,3,7,8-tetrachloro-dibenzo-p-dioxin. The mutant AL4V had 2-12 times higher activity than the wild-type P450 BM-3 towards polychlorinated dibenzo-p-dioxins. The products were hydroxylated at an unsubstituted position and/or showing migration of the chloride and were less toxic derivatives with lower than 10% toxicity of the original compounds.
Sulistyaningdyah
Woro Triarsi
WT
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Ogawa
Jun
J
Li
Qing-Shan
QS
Shinkyo
Raku
R
Sakaki
Toshiyuki
T
Inouye
Kuniyo
K
Schmid
Rolf D
RD
Shimizu
Sakayu
S
D001410:Bacillus megaterium / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D015202:Protein Engineering / Q000379:methods*; D012989:Soil Pollutants / Q000493:pharmacokinetics*; D013045:Species Specificity
D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants; D003577:Cytochrome P-450 Enzyme System