ATP-dependent and ATP-independent roles for the Rad54 chromatin remodeling enzyme during recombinational repair of a DNA double strand break.
The efficient and accurate repair of DNA double strand breaks (DSBs) is critical to cell survival, and defects in this process can lead to genome instability and cancers. In eukaryotes, the Rad52 group of proteins dictates the repair of DSBs by the error-free process of homologous recombination (HR). A critical step in eukaryotic HR is the formation of the initial Rad51-single-stranded DNA presynaptic nucleoprotein filament. This presynaptic filament participates in a homology search process that leads to the formation of a DNA joint molecule and recombinational repair of the DSB. Recently, we showed that the Rad54 protein functions as a mediator of Rad51 binding to single-stranded DNA, and here, we find that this activity does not require ATP hydrolysis. We also identify a novel Rad54-dependent chromatin remodeling event that occurs in vivo during the DNA strand invasion step of HR. This ATP-dependent remodeling activity of Rad54 appears to control subsequent steps in the HR process.
Wolner
Branden
B
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation St., Worcester, Massachusetts 01605, USA.
Peterson
Craig L
CL
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000737:chemistry*; D015139:Blotting, Southern; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004265:DNA Helicases; D004260:DNA Repair; D045643:DNA Repair Enzymes; D005277:Fenfluramine / Q000031:analogs & derivatives* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry; D016678:Genome; D006801:Humans; D006868:Hydrolysis; D047468:Immunoprecipitation; D008836:Micrococcal Nuclease / Q000378:metabolism; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D013997:Time Factors
D002843:Chromatin; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D005277:Fenfluramine; C001584:benfluorex; D000255:Adenosine Triphosphate; D004247:DNA; D008836:Micrococcal Nuclease; D000251:Adenosine Triphosphatases; C075468:RAD54 protein, S cerevisiae; D004265:DNA Helicases; D045643:DNA Repair Enzymes