title: Role of contact lens wear, bacterial flora, and mannose-induced pathogenic protease in the pathogenesis of amoebic keratitis.
abstract: The ocular surface is continuously exposed to potential pathogens, including free-living amoebae. Acanthamoeba species are among the most ubiquitous amoebae, yet Acanthamoeba keratitis is remarkably rare. The pathogenesis of Acanthamoeba keratitis is a complex, sequential process. Here we show that Acanthamoeba keratitis is profoundly affected by mannosylated proteins on the ocular surface, which stimulate the amoebae to elaborate a 133-kDa pathogenic protease. The mannose-induced protease (MIP133) mediates apoptosis of the corneal epithelium, facilitates corneal invasion, and degrades the corneal stroma. We show that contact lens wear upregulates mannosylated proteins on the corneal epithelium, stimulates MIP133 secretion, and exacerbates corneal disease. Corynebacterium xerosis, a constituent of the ocular flora, contains large amounts of mannose and is associated with Acanthamoeba keratitis. The present results show that amoebae exposed to C. xerosis produce increased amounts of MIP133 and more severe corneal disease. Oral immunization with MIP133 mitigates Acanthamoeba keratitis and demonstrates the feasibility of antidisease vaccines for pathogens that resist immune elimination.
authors.affiliation: Department of Ophthalmology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9057, USA.
authors.forename: Hassan
authors.initials: H
authors.lastname: Alizadeh
authors.forename: Sudha
authors.initials: S
authors.lastname: Neelam
authors.forename: Michael
authors.initials: M
authors.lastname: Hurt
authors.forename: Jerry Y
authors.initials: JY
authors.lastname: Niederkorn
mesh_terms: D015823:Acanthamoeba Keratitis / Q000209:etiology* / Q000503:physiopathology; D000818:Animals; D003261:Contact Lenses; D003352:Corynebacterium / Q000378:metabolism*; D006224:Cricetinae; D007700:Kinetics; D008358:Mannose / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*
chemical_list: D010447:Peptide Hydrolases; D008358:Mannose