title: Lipid compositions of human gastric fluid and epithelium: the role of sulfated lipids in gastric cytoprotection.
abstract: BACKGROUND
  Gastric sulfatide, whose carbohydrate moiety resembles that of the anti-ulcer drug sucralfate, has been shown to play a role in mucosal protection in an experimental ulcer model. To elucidate the functional significance of gastric lipids, precise determination of the lipids in human gastric fluid and epithelium was performed, and the anti-ulcer effects of all lipids in the fluid were measured in mouse ulcer models.
  
  
  METHODS
  The lipids in human gastric fluid and epithelium were analyzed by thin layer chromatography and immunostaining, and the anti-ulcer effects of gastric lipids were determined using mouse ulcer models.
  
  
  RESULTS
  Human gastric epithelium contained both sulfatide and cholesterol sulfate (CS) as sulfolipids, which were also detected in gastric fluid, showing their stable natures in the gastric fluid. Hemorrhaging in HCl-induced gastric lesions was suppressed in a dose-dependent manner by the administration of sulfolipid-containing liposomes, but suppression of stress ulcers was only accomplished with CS-containing liposomes, ie, not with sulfatide-containing ones, due to the longer retainment of CS than sulfatide in the stomach.
  
  
  CONCLUSIONS
  Among the lipids in human gastric fluid, CS was revealed to exhibit a gastroprotective activity, which was more effective than that of sulfatide.
authors.affiliation: Department of Biochemistry, Faculty of Science and Technology, Kinki University, Osaka 577-8502, Japan. iwamori@chem.kindai.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Iwamori
authors.forename: Hiroko
authors.initials: H
authors.lastname: Suzuki
authors.forename: Nobuko
authors.initials: N
authors.lastname: Ito
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Iwamori
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hanaoka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage; D002788:Cholesterol Esters / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D019610:Cytoprotection; D004195:Disease Models, Animal; D005260:Female; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006851:Hydrochloric Acid / Q000633:toxicity; D050356:Lipid Metabolism; D008055:Lipids / Q000627:therapeutic use; D008081:Liposomes; D051379:Mice; D008812:Mice, Hairless; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013433:Sulfoglycosphingolipids / Q000378:metabolism* / Q000627:therapeutic use
chemical_list: D016588:Anticarcinogenic Agents; D002788:Cholesterol Esters; D008055:Lipids; D008081:Liposomes; D013433:Sulfoglycosphingolipids; C015518:sulfolipids; C007045:cholesteryl sulfate; D006851:Hydrochloric Acid