title: First derivation in Spain of human embryonic stem cell lines: use of long-term cryopreserved embryos and animal-free conditions.
abstract: The first two human embryonic stem cell lines (VAL-1 and VAL-2) have been derived in Spain with long-term cryopreserved embryos under animal-free conditions. In the first series, 40 human embryos that had been cryopreserved at day 2 of development were thawed after >5 years. A derivation efficiency of 5% per frozen embryo or 12.5% per blastocyst was obtained.
authors.affiliation: Valencia Stem Cell Bank, Centro Superior de Alta Tecnología, Valencia, Spain. csimon@ivi.es
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.forename: Carmen
authors.initials: C
authors.lastname: Escobedo
authors.forename: Diana
authors.initials: D
authors.lastname: Valbuena
authors.forename: Olga
authors.initials: O
authors.lastname: Genbacev
authors.forename: Amparo
authors.initials: A
authors.lastname: Galan
authors.forename: Ana
authors.initials: A
authors.lastname: Krtolica
authors.forename: Angeles
authors.initials: A
authors.lastname: Asensi
authors.forename: Eva
authors.initials: E
authors.lastname: Sánchez
authors.forename: Juan
authors.initials: J
authors.lastname: Esplugues
authors.forename: Susan
authors.initials: S
authors.lastname: Fisher
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
mesh_terms: D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D013234:Stem Cells / Q000166:cytology*