First derivation in Spain of human embryonic stem cell lines: use of long-term cryopreserved embryos and animal-free conditions.
The first two human embryonic stem cell lines (VAL-1 and VAL-2) have been derived in Spain with long-term cryopreserved embryos under animal-free conditions. In the first series, 40 human embryos that had been cryopreserved at day 2 of development were thawed after >5 years. A derivation efficiency of 5% per frozen embryo or 12.5% per blastocyst was obtained.
Simón
Carlos
C
Valencia Stem Cell Bank, Centro Superior de Alta Tecnología, Valencia, Spain. csimon@ivi.es
Escobedo
Carmen
C
Valbuena
Diana
D
Genbacev
Olga
O
Galan
Amparo
A
Krtolica
Ana
A
Asensi
Angeles
A
Sánchez
Eva
E
Esplugues
Juan
J
Fisher
Susan
S
Pellicer
Antonio
A
D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D013234:Stem Cells / Q000166:cytology*