Effects of Se-depletion on glutathione peroxidase and selenoprotein W gene expression in the colon.
Selenium (Se)-containing proteins have important roles in protecting cells from oxidative damage. This work investigated the effects of Se-depletion on the expression of the genes encoding selenoproteins in colonic mucosa from rats fed diets of different Se content and in human intestinal Caco-2 cells grown in Se-adequate or Se-depleted culture medium. Se-depletion produced statistically significant (P<0.05) falls in glutathione peroxidase (GPX) 1 mRNA (60-83%) and selenoprotein W mRNA (73%) levels, a small but significant fall in GPX4 mRNA (17-25%) but no significant change in GPX2. The data show that SelW expression in the colon is highly sensitive to Se-depletion.
Pagmantidis
Vasileios
V
School of Cell and Molecular Biosciences, University of Newcastle upon Tyne, NE1 7RU, UK.
Bermano
Giovanna
G
Villette
Stephane
S
Broom
Iain
I
Arthur
John
J
Hesketh
John
J
D000818:Animals; D015152:Blotting, Northern; D018938:Caco-2 Cells; D003106:Colon / Q000201:enzymology / Q000378:metabolism*; D018076:DNA, Complementary; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D008297:Male; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000378:metabolism*; D051151:Selenoprotein W; D051140:Selenoproteins
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; C495240:SELENOW protein, human; D051151:Selenoprotein W; D051140:Selenoproteins; D005979:Glutathione Peroxidase; D012643:Selenium