title: RGDS peptide inhibits activation of lymphocytes and adhesion of activated lymphocytes to human umbilical vein endothelial cells in vitro.
abstract: The Arg-Gly-Asp (RGD) motif is known to mediate cell adhesion to several extracellular matrix components as well as cell-cell interactions. In the present study, we investigated whether the RGDS peptide interferes with cell-cell recognition-based events such as allogeneic activation of PBMC and PBMC adhesion to human umbilical vein endothelial cells (HUVEC). We show here for the first time, to our knowledge, that RGDS significantly inhibits adhesion of activated PBMC to HUVEC; in addition, RGDS inhibits PBMC allogenenic activation in human mixed lymphocyte reaction assays. Caspases played a pivotal role in both events, because preventing their activation abolished or strongly reduced the observed inhibitory effect. The RGDS antirecognition effect was strongly increased by pretreatment of HUVEC with RGDS, which affected mostly T lymphocyte adhesion to HUVEC. These results indicate that PBMC allogeneic activation, as well as reciprocal recognition between activated PBMC and endothelial cells, are RGDS-dependent events that occur through a dual effect involving anti-adhesive and caspase-dependent mechanisms. These data suggest a potential role of RGDS in cell-mediated immunity, inflammation and organ transplantation.
authors.affiliation: Laboratory of Immunology, Shock Center, IASI, CNR, Rome, Italy. aldopozzetto@hotmail.com
authors.forename: Ubaldo
authors.initials: U
authors.lastname: Pozzetto
authors.forename: Maria Simona
authors.initials: MS
authors.lastname: Aguzzi
authors.forename: Nicola
authors.initials: N
authors.lastname: Maggiano
authors.forename: Enrico
authors.initials: E
authors.lastname: Scala
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Capelli
authors.forename: Marco
authors.initials: M
authors.lastname: Castagneto
authors.forename: Maurizio C
authors.initials: MC
authors.lastname: Capogrossi
authors.forename: Franco
authors.initials: F
authors.lastname: Citterio
authors.forename: Francesco
authors.initials: F
authors.lastname: Serino
authors.forename: Antonio
authors.initials: A
authors.lastname: Facchiano
mesh_terms: D020169:Caspases / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects*; D002450:Cell Communication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D006801:Humans; D007155:Immunologic Factors; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology* / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology*; D014471:Umbilical Veins
chemical_list: D007155:Immunologic Factors; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D020169:Caspases