title: Coptidis rhizoma induces apoptosis in human colorectal cancer cells SNU-C4.
abstract: Coptidis rhizoma has been used as traditional herb medicine in gastrointestinal disorders in the Eastern Asia. We investigated whether the anticancer effects of the C. rhizoma induced apoptosis on human colorectal cancer cells SNU-C4. The cytotoxic effect of C. rhizoma was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. To determine apoptotic cell death, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) and caspase-3 enzyme assay were performed. In this study, C. rhizoma treatment (100 microg/ml) revealed typical morphological apoptotic features. Additionally, C. rhizoma treatment (100 microg/ml) increased levels of BAX and CASPASE-3, and decreased levels of BCL-2. Caspase-3 enzyme activity by treatment of C. rhizoma (100 microg/ml) also significantly increased compared to the control (p < 0.05). These data indicate that C. rhizoma caused cell death by apoptosis through caspase pathways on human colorectal cancer cells SNU-C4.
authors.affiliation: Department of Dental Hygiene, Hanseo University, Seosan, Korea.
authors.forename: Youn Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Soon Ah
authors.initials: SA
authors.lastname: Kang
authors.forename: Mee Suk
authors.initials: MS
authors.lastname: Hong
authors.forename: Hae Jeong
authors.initials: HJ
authors.lastname: Park
authors.forename: Mi-Ja
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hi-Jun
authors.initials: HJ
authors.lastname: Park
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D015179:Colorectal Neoplasms; D000089042:Coptis chinensis; D017931:DNA Primers; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D007723:Korea; D016133:Polymerase Chain Reaction
chemical_list: D016588:Anticarcinogenic Agents; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; C107393:Coptidis rhizoma extract; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases