In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury.
Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.
Souza
Elen Mello de
EM
Laboratório de Biologia Celular, Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
Henriques-Pons
Andrea
A
Bailly
Christian
C
Lansiaux
Amelie
A
Araújo-Jorge
Tânia Cremonine
TC
Soeiro
Maria de Nazaré Correia
Mde N
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002318:Cardiovascular Diseases / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D018929:Cell Culture Techniques; D004796:Clinical Enzyme Tests; D003402:Creatine Kinase / Q000032:analysis*; D052279:Creatine Kinase, MB Form; D052283:Creatine Kinase, MM Form; D007527:Isoenzymes / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D032383:Myocytes, Cardiac / Q000201:enzymology* / Q000648:ultrastructure; D012680:Sensitivity and Specificity
D015415:Biomarkers; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form; D052283:Creatine Kinase, MM Form