Donor age affects differentiation of rat ventral mesencephalic stem cells.
This study examined the effect of gestational age of rat ventral mesencephalon (VM) on the characteristics of neurospheres generated from this region and on the yield of each cell type after differentiation of these neurospheres. Neurospheres generated from embryonic day (E) 12 and E13 VM had significantly larger diameters and volumes than those from E14 VM. Subsequent differentiation of these neurospheres resulted in decreasing yield of neurones (E12>E13>E14) and increasing yields of both astroglia and oligodendroglia (E12<E13<E14) with increasing donor age. Dopaminergic neurones were generated in low numbers from E12 and E13 cultures, and were virtually absent in E14 cultures. This study suggests that gestational age is a critical factor in the selection of tissue to generate particular cell types from neural stem cells. This has important implications for the use of neural stem cells in transplantation approaches to neurodegenerative diseases.
O' Keeffe
Gerard W
GW
Department of Neuroscience/Anatomy, Biosciences Research Institute, National University of Ireland Cork (NUIC), College Road, Cork, Ireland.
Sullivan
Aideen M
AM
D000367:Age Factors; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D015415:Biomarkers; D016380:Brain Tissue Transplantation / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D019636:Neurodegenerative Diseases / Q000628:therapy; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018874:Spheroids, Cellular / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013378:Substantia Nigra / Q000166:cytology / Q000196:embryology / Q000502:physiology; D014019:Tissue Donors; D017557:Ventral Tegmental Area / Q000166:cytology / Q000196:embryology / Q000502:physiology
D015415:Biomarkers; D009419:Nerve Tissue Proteins; D004298:Dopamine