Transformed roots of Crepis capillaris--a [corrected] sensitive system for the evaluation of the clastogenicity of abiotic agents.
The presence of a large number of pollutants, including mutagenic agents in the environment is a problem of a major concern. Rapid progress in plant biotechnology, especially in the development of cell transformation methods, including the production of transformed roots -- 'hairy roots' -- has opened new possibilities to use transformed root cultures in plant bioassays for the evaluation mutagenic effects of different agents. We have used Crepis capillaris hairy roots for evaluation of cytogenetic effects of mutagenic treatment. Effects of maleic acid hydrazide (MH) and X-ray treatment were analysed in chromosomal aberration, sister chromatid exchange (SCE) and TUNEL tests. Comparison of cytogenetic effects in hairy roots and roots of seedlings showed a much higher sensitivity of hairy roots, which makes them convenient material for monitoring DNA damage after mutagenic treatment.
Juchimiuk
Jolanta
J
Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland. juchimiu@us.edu.pl
Maluszynska
Jolanta
J
D002274:Carcinogens, Environmental / Q000633:toxicity; D002869:Chromosome Aberrations; D027468:Crepis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D046508:Culture Techniques; D053938:DNA Fragmentation; D006540:Herbicides / Q000633:toxicity; D020287:In Situ Nick-End Labeling; D008300:Maleic Hydrazide / Q000633:toxicity; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects; D012854:Sister Chromatid Exchange; D014965:X-Rays
D002274:Carcinogens, Environmental; D006540:Herbicides; D009153:Mutagens; D008300:Maleic Hydrazide