title: LCC15-MB cells are MDA-MB-435: a review of misidentified breast and prostate cell lines.
abstract: Current stocks of the LCC15-MB cell line, which we originally isolated from a human breast-bone metastasis, were found to be genetically matched to the MDA-MB-435 cell line from the Lombardi Cancer Center (MDA-MB-435-LCC) using comparative genomic hybridisation, DNA microsatellite analysis and chromosomal number. LCC15-MB stocks used for our previously published studies as well as the earliest available LCC15-MB cells also showed identity to MDA-MB-435-LCC cells. The original karyotype reported for LCC15-MB cells was considerably different to that of MDA-MB-435 cells, indicating that the original LCC15-MB cells were lost to contamination by MDA-MB-435-LCC cells. Chromosome number is the simplest test to distinguish original LCC15-MB cells (n approximately 75) from MDA-MB-435 (n approximately 52). Collectively, our results prove that LCC15-MB cells currently available are MDA-MB-435 cells and we suggest their re-designation as MDA-MB-435-LCC15 cells. We also review the known misclassification of breast and prostate cancer cell lines to date and have initiated a register maintained at http://www.svi.edu.au/cell_lines_registry.doc.
authors.affiliation: Invasion and Metastasis Unit, University of Melbourne, Department of Surgery, St. Vincent's Hospital, 29 Regent St., Fitzroy, 3065, Australia. rik@medstv.unimelb.edu.au
authors.forename: Erik W
authors.initials: EW
authors.lastname: Thompson
authors.forename: Mark
authors.initials: M
authors.lastname: Waltham
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Ramus
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Hutchins
authors.forename: Jane E
authors.initials: JE
authors.lastname: Armes
authors.forename: Ian G
authors.initials: IG
authors.lastname: Campbell
authors.forename: Elizabeth D
authors.initials: ED
authors.lastname: Williams
authors.forename: Phillip R
authors.initials: PR
authors.lastname: Thompson
authors.forename: James M
authors.initials: JM
authors.lastname: Rae
authors.forename: Michael D
authors.initials: MD
authors.lastname: Johnson
authors.forename: Robert
authors.initials: R
authors.lastname: Clarke
mesh_terms: D001943:Breast Neoplasms / Q000145:classification* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D009693:Nucleic Acid Hybridization; D011003:Ploidies; D011471:Prostatic Neoplasms / Q000145:classification* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology