A novel family of glucosyl 1,5-anhydro-d-fructose derivatives synthesised by transglucosylation with dextransucrase from Leuconostoc mesenteroides NRRL B-512F.
1,5-Anhydro-d-fructose (AF), a metabolite of starch/glycogen degradation, is a good antioxidant. With the prospect of increasing its applications and use as a food ingredient, AF glucosylation catalysed by the dextransucrase from Leuconostoc mesenteroides NRRL B-512F was performed in the presence of sucrose. This led to AF glucosylated derivatives containing alpha-(1-->6) linkages named 1,5-anhydro-d-fructo-glucooligosaccharides (AFGOS). LC-MS analyses showed that AFGOS with a degree of polymerisation (DP) of up to 7 were synthesised. The amount of AFGOS produced and the average DP increased by using a high sucrose/AF molar ratio and high total sugar concentration. AFGOS were proved to present antioxidant properties quite similar to AF.
Richard
Gaëtan
G
Laboratoire de Biotechnologie-Bioprocédés UMR CNRS 5504, UMR INRA 792, INSA DGBA 135 avenue de Rangueil 31077 Toulouse Cedex 04, France.
Yu
Shukun
S
Monsan
Pierre
P
Remaud-Simeon
Magali
M
Morel
Sandrine
S
D000975:Antioxidants / Q000737:chemistry*; D004187:Disaccharides / Q000138:chemical synthesis*; D005947:Glucose / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry*; D007934:Leuconostoc / Q000201:enzymology*
C488015:1,4-anhydro-3-O-glucopyranosylfructose; D000975:Antioxidants; D004187:Disaccharides; D005964:Glucosyltransferases; C020025:dextransucrase; D005947:Glucose