A protocol for direct sequencing of multiple gene specific PCR products from Discula umbrinella, a fungal endophyte, utilizing bufferless precast electrophoresis.
A protocol for direct sequencing of polymerase chain reaction (PCR) products from mycelia of Discula umbrinella, a fungal endophyte, using bufferless electrophoresis is described. This improved method allows researchers to conduct high-capacity screening of multiple gene regions for fungal endophytes applicable to microbial ecology and population genetic studies.
Cohen
Susan D
SD
Policy and Program Development, U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Riverdale, MD, 20737, USA. susan.d.cohen@aphis.usda.gov
D002687:Chitin Synthase / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D003904:Mitosporic Fungi / Q000235:genetics*; D020648:Peptide Elongation Factor 1 / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D029963:Quercus / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D002687:Chitin Synthase