title: Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy.
abstract: Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Raman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Raman measurements. Autofluorescence signals from tissues, which interfere with the Raman signals, were greatly reduced using a confocal slit adjustment. Distinct Raman band differences between normal and BCC tissues for the amide I mode and the PO2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Raman depth profiling technique. We propose that confocal Raman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.
authors.affiliation: Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Choi
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Hoeil
authors.initials: H
authors.lastname: Chung
authors.forename: Dae-Gab
authors.initials: DG
authors.lastname: Gweon
authors.forename: Jeunghee
authors.initials: J
authors.lastname: Park
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sangyong
authors.initials: S
authors.lastname: Park
authors.forename: Chil-Hwan
authors.initials: CH
authors.lastname: Oh
mesh_terms: D002280:Basal Cell Carcinoma / Q000473:pathology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000166:cytology* / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology*; D013059:Spectrum Analysis, Raman