title: UVB-induced leucocyte trafficking in the epidermis of photosensitive lupus erythematosus patients: normal depletion of Langerhans cells.
abstract: BACKGROUND
  The pathogenic mechanisms of UV-induced skin lesions of lupus erythematosus (LE) are unknown. In a recent study of pathogenic mechanisms of polymorphic light eruption (PLE), significantly more Langerhans cells (LCs) persisted in the epidermis after UVB overexposure than in healthy individuals. Interestingly, the same phenomenon was observed in one subacute cutaneous lupus erythematosus (SCLE) patient. It could therefore be hypothesized that both photodermatoses share a common pathogenic mechanism of photosensitivity. In the present study, we tested this hypothesis by investigating leucocyte trafficking in the initial phase of cutaneous LE after intense UVB exposure.
  
  
  METHODS
  In 22 photosensitive LE patients (12 chronic discoid lupus erythematosus, seven systemic lupus erythematosus and three SCLE) and nine age/sex-matched controls, uninvolved buttock skin was exposed to six minimal erythemal dose (MED) UVB radiation. Subsequently, biopsies were taken after 24, 48 and 72 h, and one control biopsy was taken from unirradiated skin. Skin sections were stained for the presence of LCs, neutrophils and macrophages. Areal percentages of positively stained cells within the epidermis were quantified and compared between the patients and controls.
  
  
  RESULTS
  A gradual decrease of epidermal LCs and a gradual increase of epidermal neutrophils and macrophages at several timepoints after six MED irradiation was observed equally in both LE patients and controls.
  
  
  CONCLUSIONS
  Immunohistopathology of irradiated uninvolved skin of photosensitive LE patients did not reveal the same pathologic trafficking of LCs and neutrophils as described for PLE patients. We conclude that different mechanisms are operative in the pathogenesis of PLE and photosensitive LE.
authors.affiliation: Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands. a.s.janssens@lumc.nl
authors.forename: Artiena Soe
authors.initials: AS
authors.lastname: Janssens
authors.forename: Eileen E L O
authors.initials: EE
authors.lastname: Lashley
authors.forename: Coby J
authors.initials: CJ
authors.lastname: Out-Luiting
authors.forename: Rein
authors.initials: R
authors.lastname: Willemze
authors.forename: Stan
authors.initials: S
authors.lastname: Pavel
authors.forename: Frank R
authors.initials: FR
authors.lastname: de Gruijl
mesh_terms: D000293:Adolescent; D000328:Adult; D004817:Epidermis / Q000473:pathology / Q000528:radiation effects*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000473:pathology / Q000528:radiation effects*; D007962:Leukocytes / Q000166:cytology / Q000528:radiation effects*; D008027:Light; D008178:Lupus Erythematosus, Cutaneous / Q000378:metabolism* / Q000473:pathology*; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000473:pathology*; D012867:Skin / Q000473:pathology; D013997:Time Factors; D014466:Ultraviolet Rays