Increased efficiency of phorbol ester-induced lytic reactivation of Kaposi's sarcoma-associated herpesvirus during S phase.
Expression of Kaposi's sarcoma-associated herpesvirus (KSHV) lytic genes is thought to be essential for the establishment and progression of KSHV-induced diseases. The inefficiency of lytic reactivation in various in vitro systems hampers the study of lytic genes in the context of whole virus. We report here increased expression of KSHV lytic genes and increased release of progeny virus when synchronized cultures of body cavity-based lymphoma-1 cells are treated with a phorbol ester during S phase of the cell cycle.
McAllister
Shane C
SC
Vaccine and Gene Therapy Institute, Oregon Health and Science University, 505 NW 185th Ave., Beaverton, OR 97006, USA.
Hansen
Scott G
SG
Messaoudi
Ilhem
I
Nikolich-Zugich
Janko
J
Moses
Ashlee V
AV
D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts / Q000821:virology; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D005814:Genes, Viral / Q000502:physiology; D019288:Herpesvirus 8, Human / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D010703:Phorbol Esters / Q000494:pharmacology*; D016196:S Phase / Q000502:physiology*; D012514:Sarcoma, Kaposi / Q000821:virology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D004279:DNA, Viral; D010703:Phorbol Esters