Stability of chitosan-pDNA complex powder prepared by supercritical carbon dioxide process.
The present study examined the stability of a gene in powders prepared with supercritical carbon dioxide (CO(2)) from the viewpoints of the ternary structure of DNA and in vivo transfection potential. An aqueous chitosan-pCMV-Luc complex solution containing mannitol was injected into the stream of a supercritical CO(2)/ethanol admixture to precipitate a gene powder. The obtained gene powders and gene solutions were placed in stability chambers at 25 or 40 degrees C for 4 weeks. The integrity and transfection potency of the gene were examined by electrophoresis and in vivo pulmonary transfection study in mice. The supercritical CO(2) process decreased the supercoiled DNA during the manufacturing process; however, the decrease in the remaining supercoiled and open circular DNA in the powders during storage was much slower than that in solutions. In addition, the powders had higher transfection potency than the solutions containing the same amount of DNA. The effect of chitosan on the stability of DNA in solutions was not obvious in the solutions but it improved the stability of DNA in powders during manufacturing and storage. Thus, a gene powder with a cationic vector is a promising ready-to-use formulation for inhalation therapy of pulmonary diseases.
Okamoto
Hirokazu
H
Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan. okamotoh@ccmfs.meijo-u.ac.jp
Sakakura
Yuki
Y
Shiraki
Keiko
K
Oka
Kumiko
K
Nishida
Seiko
S
Todo
Hiroaki
H
Iida
Kotaro
K
Danjo
Kazumi
K
D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000032:analysis / Q000138:chemical synthesis*; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000138:chemical synthesis* / Q000235:genetics; D004270:DNA, Circular / Q000032:analysis / Q000138:chemical synthesis; D004278:DNA, Superhelical / Q000032:analysis / Q000138:chemical synthesis; D004355:Drug Stability; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D010957:Plasmids / Q000032:analysis / Q000138:chemical synthesis / Q000235:genetics; D011208:Powders
D004270:DNA, Circular; D004278:DNA, Superhelical; D011208:Powders; D002245:Carbon Dioxide; D004247:DNA; D048271:Chitosan