Cell-based biosensors based on light-addressable potentiometric sensors for single cell monitoring.
Cell-based biosensors incorporate cells as sensing elements that convert changes in immediate environment to signals for processing. This paper reports an investigation on light-addressable potentiometric sensor (LAPS) to be used as a possible cell-base biosensor that will enable us to monitor extracellular action potential of single living cell under stimulant. In order to modify chip surface and immobilize cells, we coat a layer of poly-L-ornithine and laminin on surface of LAPS chip on which rat cortical cells are grown well. When 10 microg/ml acetylcholine solution is administrated, the light pointer is focused on a single neuronal cell and the extracellular action potential of the targeted cell is recorded with cell-based biosensor based on LAPS. The results demonstrate that this kind of biosensor has potential to monitor electrophysiology of living cell non-invasive for a long term, and to evaluate drugs primarily.
Xu
Gaixia
G
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of the Ministry of Education, Department of Biomedical Engineering, PO Box 1590, Zhejiang University, Hangzhou 310027, China.
Ye
Xuesong
X
Qin
Lifeng
L
Xu
Ying
Y
Li
Yan
Y
Li
Rong
R
Wang
Ping
P
D000109:Acetylcholine / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D018914:Cells, Immobilized; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008027:Light; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D055095:Optics and Photonics / Q000295:instrumentation; D018462:Photobiology / Q000295:instrumentation / Q000379:methods; D051381:Rats
D000109:Acetylcholine