title: Isolation and characterization of a cDNA encoding phytochrome A in the non-photosynthetic parasitic plant, Orobanche minor Sm.
abstract: In this study, the isolation and characterization of a phytochrome A (PHYA) homologous cDNA (OmPHYA) in the non-photosynthetic holoparasitic plant Orobanche minor are described. The present findings provide the first report of the presence of a PHYA homolog in the holoparasite. This study found that OmPHYA is of similar size to the other PHYAs of green plants and shows 72, 77, and 77% amino acid sequence identity with PHYA in Arabidopsis, potato, and tobacco respectively. The OmPHYA contains a conserved chromophore attachment cysteine at position 323. Although OmPHYA shows high sequence identity with other PHYAs in green plants, 13 amino acid substitutions located in both the N and C-terminal domains are observed (a total of 26 amino acids). OmPHYA is encoded by a single gene within the O. minor genome. The abundance of the OmPHYA transcript as well as nuclear translocation of OmphyA occurs in a light-dependent manner.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
authors.forename: Chitra
authors.initials: C
authors.lastname: Trakulnaleamsai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okazawa
authors.forename: Chung-Il
authors.initials: CI
authors.lastname: An
authors.forename: Shin'ichiro
authors.initials: S
authors.lastname: Kajiyama
authors.forename: Ei'ichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoneyama
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Akio
authors.initials: A
authors.lastname: Kobayashi
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D018744:DNA, Plant / Q000737:chemistry / Q000302:isolation & purification*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D031672:Orobanche / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D010834:Phytochrome / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D051942:Phytochrome A; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; C497973:PHYA protein, Arabidopsis; D051942:Phytochrome A; D010834:Phytochrome; D017346:Protein Serine-Threonine Kinases