[Effect of tanshinone microemulsion on reversing MDR in human tumor cells].
OBJECTIVE
To study the effect of tanshinone microemulsion (Tan-M) on the cytotoxicity to human leukemia-cell-line (K562/ADM) and the reversion of MDR in vitro.


METHODS
Microemulsion being supposed as the control group, MT method is adopted to test cytotoxicity and the reverse of MDR.


RESULTS
Obvious cytotoxicity to K562/ADM was observed for tan-M. Cell non-toxic dosage (growth quotiety > 95%) of Tan-M is 0.2 microg x mL(-1). Low toxic dosage (growth quotiety 85-90%) was 0.7 microg x mL(-1). Cell non-toxic dosage of was 0.7 microg x mL(-1) and low toxic dosage was 1.2 microg x mL(-1). Cell non-toxic dosage of Tan-M (0.2 microg x mL(-1)) significantly lowered the IC50 of K562/ADM by ADM (P < 0.01), and reversed MDR was 3.88 times. Low toxic dosage of Tan-M reversed MDR was 3.97 times. E-M (0.2 microg x mL(-1)) reversed MDR was 2.62 times.


CONCLUSIONS
The result indicates that tanshinone microemulsion possesses cell-toxic effects on human leukemia cell-line and may reverse MDR of tumor cells.
Fan
Qing
Q
Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China. FQ731@sina.com.cn
Fan
Guang-jun
GJ
Yang
Pei-man
PM
Zhao
Jin-yao
JY
D045784:Abietanes; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D004655:Emulsions; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D010616:Phenanthrenes / Q000008:administration & dosage / Q000494:pharmacology*
D045784:Abietanes; D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D010616:Phenanthrenes; C021751:tanshinone; D004317:Doxorubicin