Proteome analysis of skin distinguishes acute guttate from chronic plaque psoriasis.
Psoriasis is a disease with considerable heterogeneity in clinical presentation. This is the first study using two-dimensional gel electrophoresis to compare global protein expression patterns in lesional and non-lesional skin from subjects with acute guttate psoriasis associated with streptococcal throat infection and chronic plaque psoriasis. Samples from experimentally induced contact eczema and normal skin from healthy controls were also included. Proteins with statistically significant differences in expression were used in hierarchical cluster analyses resulting in separation of the different samples into groups. Chronic plaque and guttate psoriasis samples were distinctly separated, indicating that they represent discrete phenotypes at the protein expression level. Interestingly, there was a trend in which guttate psoriasis lesions clustered closer to eczema than to chronic plaque psoriasis lesions, indicating that the duration of the inflammatory reaction may affect clustering. Several of the differentially expressed proteins were identified by mass spectrometry.
Carlén
Lina M
LM
Department of Dermatology, Karolinska University Hospital, Stockholm, Sweden.
Sánchez
Fabio
F
Bergman
Ann-Charlotte
AC
Becker
Susanne
S
Hirschberg
Daniel
D
Franzén
Bo
B
Coffey
Jonathan
J
Jörnvall
Hans
H
Auer
Gert
G
Alaiya
Ayodele A
AA
Ståhle
Mona
M
D000208:Acute Disease; D002908:Chronic Disease; D016000:Cluster Analysis; D003937:Diagnosis, Differential; D004485:Eczema / Q000175:diagnosis / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D010641:Phenotype; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D011565:Psoriasis / Q000175:diagnosis* / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
D020543:Proteome