[The influence of theophilline on oxygen metabolism of neutrophils in vitro].
Theophylline is a drug used in the treatment of obstructive pulmonary disease and a strong immunomodulator and has anti-inflammatory potential. However, its mechanism of definite action is still poorly defined. In the present study the effect of teophylline on the in vitro formation and release of reactive oxygen species in polymorphonuclear neutrophils (PMN) was studied at concentration of 0.9-130 microg/ml. The neutrophils oxygen metabolism was assessed with methods: luminol-dependent chemiluminescence, cytochrome C reduction, phenol red oxidation, and Griess reaction. In vitro theophylline treatment most effectively inhibit neutrophil hydrogen peroxide and nitric oxide generation (at 90 and 130 microg/ml), superoxide production (at 1.3 microg/ml), and diminish chemiluminescence (at the smallest concentrations 0.9 and 1.3 microg/m). We concluded that the effects of theophylline on granulocyte function are likely to contribute to the drug's clinical efficacy.
Zielińska
Małgorzata
M
Zakład Biochemii, Katedry Biochemii Farmaceutycznej AM im. K. Marcinkowskiego w Poznaniu. mzielin@am.poznan.pl
Puszczewicz
Mariusz
M
D001993:Bronchodilator Agents / Q000494:pharmacology*; D002151:Calorimetry; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009504:Neutrophils / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology*
D001993:Bronchodilator Agents; D017382:Reactive Oxygen Species; D013806:Theophylline; D010100:Oxygen