title: Influence of immobilization parameters on growth and lactic acid production by Streptococcus thermophilus and Lactobacillus bulgaricus co-immobilized in calcium alginate gel beads.
abstract: Streptococcus thermophilus and Lactobacillus bulgaricus were co-immobilized in different systems with varying calcium (0.1-1.5 M) and alginate (1-2%, w/v) concentrations. Highest lactic acid production was 35 g l(-1) when both bacteria were in high viscosity beads (1%, w/v alginate) hardened in 0.1 M CaCl2 . The gel bead composition affected size and distribution of entrapped lactic acid bacteria.
authors.affiliation: Departamento de Química y CC.MM., (Area de Bioquímica), Facultad de Ciencias Experimentales, Universidad de Huelva, Campus del Carmen, 21017 Huelva, Spain. garbayo@uhu.es
authors.forename: I
authors.initials: I
authors.lastname: Garbayo
authors.forename: C
authors.initials: C
authors.lastname: Vílchez
authors.forename: J M
authors.initials: JM
authors.lastname: Vega
authors.forename: J E
authors.initials: JE
authors.lastname: Nava-Saucedo
authors.forename: J N
authors.initials: JN
authors.lastname: Barbotin
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D018914:Cells, Immobilized / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods*; D005782:Gels / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000502:physiology*; D008422:Materials Testing; D008863:Microspheres; D048249:Streptococcus thermophilus / Q000502:physiology*; D014783:Viscosity
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D005782:Gels; D006603:Hexuronic Acids; D019344:Lactic Acid; D020723:Glucuronic Acid