Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1.
Importin alpha1/Rch1, an importin alpha family member, mediates the nuclear import of karyophilic proteins. The present study reports on a monoclonal antibody (MAb) directed against mammalian importin alpha1/Rch1, which was produced by the hybridization of mouse myeloma cells with lymph node cells of an immunized rat. The MAb 1A6 specifically recognized importin alpha1/Rch1 among the importin alpha isoforms, as evidenced by Western blotting. Furthermore, 1A6 detected importin alpha1/Rch1 in HeLa cells by immunofluorescence. This MAb will be useful in immunolocalization and immunoblotting experiments, conducted on different tissue types, to determine the levels of expression of importin alpha1/Rch1 throughout development, as well as further analyses of the biological function and cellular dynamics of this protein.
Kamikubo
Yuji
Y
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Osaka, Japan.
Sakaguchi
Naoko
N
Shikata
Keiji
K
Furuta
Maiko
M
Miyamoto
Yoichi
Y
Imamoto
Naoko
N
Yoneda
Yoshihiro
Y
Ogino
Kenji
K
Tachibana
Taro
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002467:Cell Nucleus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D020033:Protein Isoforms / Q000276:immunology; D051381:Rats; D028901:alpha Karyopherins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D020033:Protein Isoforms; D028901:alpha Karyopherins; C087978:karyopherin alpha 2