title: Antigenicity of two turkey astrovirus isolates.
abstract: Astroviruses are positive-sense single-stranded RNA viruses. These viruses cause gastroenteritis in humans and in a variety of animal species, including turkey poults. Only human astroviruses are well characterized antigenically. In the current study, two turkey astrovirus isolates, TAstV1987 and TAstV2001, were antigenically compared using cross-neutralization tests in turkey embryos, as well as cross-reactivity of the two isolates by an enzyme-linked immunosorbent assay (ELISA). The antigenic relatedness values (R) were calculated using the Archetti and Horsfall formula. The R value based on the cross-neutralization tests was 0.56%, which indicates that TAstV1987 and TAstV2001 belong to different serotypes; the R value of the two viruses based on ELISA was 70.7%, which suggests these two viruses share common antigen(s).
authors.affiliation: Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.forename: Y M
authors.initials: YM
authors.lastname: Saif
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D001257:Mamastrovirus / Q000145:classification* / Q000276:immunology*; D009500:Neutralization Tests; D012703:Serotyping / Q000379:methods / Q000662:veterinary*; D014422:Turkeys / Q000821:virology*
chemical_list: D000914:Antibodies, Viral