Chiral separation of NBD-amino acids by ligand-exchange micro-channel electrophoresis.
The chiral separation of amino acid derivatives by ligand-exchange electrophoresis in a microchannel chip was performed for the first time. A Cu(II) complex with L-prolinamide was used as a chiral selector. The migration behaviors of eleven NBD-DL-amino acids were investigated by ligand-exchange capillary electrophoresis (LE-CE). The enantiomer of five NBD-amino acids (Ser, Thr, Val, Phe and His) could be separated by LE-CE using a 20 mM ammonium acetate buffer (pH 9.0) containing 10 mM copper acetate, 20 mM L-prolinamide and 1 mM SDS. NBD-His was eluted in the order D-form and L-form, while the elution order of another enantiomers was L-form and D-form. Under this condition, the enantioseparation of these five NBD-amino acids by ligand-exchange microchip electrophoresis (LE-ME) was investigated using a glass microchip. The enantioseparation of NBD-Ser, -Thr and -His could be successfully accomplished by LE-ME. LE-ME was superior to LE-CE in terms of the short migration time and a good enantiomeric separation.
Nakajima
Hizuru
H
Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan. nakajima-hizuru@c.metro-u.ac.jp
Kawata
Katsuhiko
K
Shen
Hui
H
Nakagama
Tatsuro
T
Uchiyama
Katsumi
K
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives*; D000596:Amino Acids / Q000302:isolation & purification*; D003300:Copper; D046209:Electrophoresis, Microchip; D008024:Ligands; D011392:Proline / Q000031:analogs & derivatives*; D013237:Stereoisomerism
D000596:Amino Acids; D008024:Ligands; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D003300:Copper; D011392:Proline; D009327:4-Chloro-7-nitrobenzofurazan; C056291:prolinamide