title: Salmonella-induced filament formation is a dynamic phenotype induced by rapidly replicating Salmonella enterica serovar typhimurium in epithelial cells.
abstract: Salmonella enterica serovar Typhimurium has the fascinating ability to form tubular structures known as Salmonella-induced filaments (Sifs) in host cells. Here, we show that the prevalence of the Sif phenotype in HeLa cells is affected by host cell density, growth, and the multiplicity of infection. Sif formation was observed in cells that displayed rapid intracellular bacterial replication and was found to be dynamic, being maximal 8 to 10 h postinfection and declining thereafter. The virulence factors SpvB and SseJ were found to negatively modulate Sif formation. Our findings demonstrate the complex and dynamic nature of the Sif phenotype.
authors.affiliation: Infection, Immunity, Injury and Repair (IIIR) Program, Hospital for Sick Children, 555 University Ave., Toronto, Ontario, Canada M5G 1X8.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Birmingham
authors.forename: Xiuju
authors.initials: X
authors.lastname: Jiang
authors.forename: Maikke B
authors.initials: MB
authors.lastname: Ohlson
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Miller
authors.forename: John H
authors.initials: JH
authors.lastname: Brumell
mesh_terms: D002455:Cell Division / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D012480:Salmonella Infections; D012486:Salmonella typhimurium / Q000166:cytology / Q000502:physiology*; D013997:Time Factors