title: [Effect of insulin, cAMP, and dexamethasone on phosphoenolpyruvate carboxykinase gene promoter in vitro].
abstract: OBJECTIVE
  To study the effect of insulin, cyclic adenosine monophosphate (cAMP), and dexamethasone (DEX) on 550 bp (-600 -/+ 69) fragment of phosphoenolpyruvate carboxykinase (PEPCK) gene promoter by reporter gene.
  
  
  METHODS
  The recombinant pGL2-PEPCK-Luc and the control plasmid pSV-beta-Galactosidase were co-transfected to rat hepatoma cell line (CBRH7919) by lipofectin. By measuring luciferase activity, we evaluated in vitro regulation of PEPCK gene promoter on reporter gene transcription.
  
  
  RESULTS
  cAMP and DEX stimulated PEPCK promoter obviously; meanwhile, they also had accumulative effects. At different physiological concentrations, insulin had a suppressive effect on PEPCK promoter, which was dose-independent.
  
  
  CONCLUSIONS
  There is a perfect feedback mechanism for PEPCK promoter in hepatoma cell. 550 bp (-600 -/+ 69) fragment of PEPCK may be a candidate gene in the gene therapy of diabetes.
authors.affiliation: Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. fengkai@medmail.com.cn
authors.forename: Kai
authors.initials: K
authors.lastname: Feng
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D000242:Cyclic AMP / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000187:drug effects* / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D007328:Insulin; D011994:Recombinant Proteins; D003907:Dexamethasone; D000242:Cyclic AMP; D008156:Luciferases; D010729:Phosphoenolpyruvate Carboxykinase (GTP)