title: [Immune potency of recombinant adeno-associated virus combined with recombinant adenovirus vaccine containing HIV-1 gp120].
abstract: OBJECTIVE
  To study the immune effect of recombinant adeno-associated virus (rAAV) combined with recombinant adenovirus (rAdV) vaccine in BALB/c mice.
  
  
  METHODS
  The codon-modified HIV-1 gp120 gene was inserted into plasmid of adeno-associated virus and adenovirus vector separately. Then the rAAV and rAdV vaccines were constructed. BALB/c mice were immunized with rAAV and rAdV vaccines in different administration scheme. The IgG antibody was detected by ELISA and CTL response was detected by intracellular cytokine stain assay.
  
  
  RESULTS
  Both rAAV and rAdV vaccine could express gp120 gene; the mice primed with rAAV at week 0, 2 and boosted with rAdV at week 5, 14 and 20 elicited the strongest gp120 specific CTL and IgG antibody response.
  
  
  CONCLUSIONS
  The mice primed with rAAV and boosted with rAdV could elicit specific CTL response and IgG antibody.
authors.affiliation: Department of Oncogenic Virus and HIV, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Feng
authors.forename: Shuang-qing
authors.initials: SQ
authors.lastname: Yu
authors.forename: Guo-min
authors.initials: GM
authors.lastname: Chen
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Wu
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Zuo
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Dong
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D000229:Dependovirus / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D015497:HIV-1; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011995:Recombination, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology* / Q000378:metabolism
chemical_list: D015699:HIV Envelope Protein gp120; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D007371:Interferon-gamma