title: Cleavage of integrin by mu-calpain during hypoxia in human endometrial cells.
abstract: OBJECTIVE
  The distribution and activation of mu-calpain and possible cleavage of integrin in human endometrial cells under hypoxic condition were investigated.
  
  
  METHODS
  Human endometrial epithelial and stromal cells were subjected to hypoxia, and subsequently used for immunostaining and western blot analysis.
  
  
  RESULTS
  The proform of mu-calpain was detected in the cytoplasm of normal cells, and displayed a substantial decrease after hypoxia. Conversely, the active form of mu-calpain was not detected in normal cells, but was abundant after hypoxia. The cytoplasmic domain of integrin beta3 was also detected in the cytoplasm of endometrial cells. Western blot analysis confirmed that both the proform of mu-calpain and the integrin beta3 cytoplasmic domain decreased during hypoxia.
  
  
  CONCLUSIONS
  Mu-calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin beta3 cytoplasmic domain may give some adverse effects to the function of human endometrium.
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan. k-aoyama@xd5.so-net.ne.jp
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Aoyama
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Tamao
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Mayumi S
authors.initials: MS
authors.lastname: Ogasawara
authors.forename: Katsuo
authors.initials: K
authors.lastname: Ikuta
authors.forename: Koji
authors.initials: K
authors.lastname: Aoki
authors.forename: Klas
authors.initials: K
authors.lastname: Blomgren
authors.forename: Kaoru
authors.initials: K
authors.lastname: Suzumori
mesh_terms: D015153:Blotting, Western; D002154:Calpain / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D039661:Integrin beta3 / Q000378:metabolism*; D018613:Microscopy, Confocal
chemical_list: D039661:Integrin beta3; D002154:Calpain; C449002:mu-calpain