Sodium butyrate induces apoptosis in MSN neuroblastoma cells in a calcium independent pathway.
Sodium butyrate (NaBt), a histone deacetylase inhibitor, can cause apoptosis in a number of cancer cells. However, the mechanism of this action is poorly understood. Increased intracellular [Ca(2+)] level has been suggested as a likely mechanism, but there is little corroborating data. In this report we provide evidence that NaBt-treated MSN neuroblastoma cells undergo massive apoptosis in the presence of serum and regardless of external or internal [Ca(2+)] levels. Presented data suggest that apoptotic effect of NaBt is both time- and dose-dependent (LD50 1 mM); and that, presence of serum or cAMP, a second messenger molecule that modulates the apoptotic program in a wide variety of cells could not circumvent the apoptotic effect of NaBt. Our findings suggest that NaBt-induced apoptosis in MSN neuroblastoma cells occurs via a pathway that is independent of Ca(2+) flux, intracellular [Ca(2+)] or cAMP levels. Further, we also present data that exclude a role for PKC or histones acetylation.
Rozental
R
R
Department of Cell Biology & Anatomy, New York Medical College, Valhalla, New York 10595, USA.
Faharani
R
R
Yu
Y
Y
Johnson
J M
JM
Chan
S O
SO
Chiu
F C
FC
D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000473:pathology*; D003994:Bucladesine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor; D053938:DNA Fragmentation; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D013778:Tetrazolium Salts; D013806:Theophylline / Q000494:pharmacology; D013844:Thiazoles
D002087:Butyrates; D010726:Phosphodiesterase Inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles; D003994:Bucladesine; D013806:Theophylline; C022616:thiazolyl blue; D002118:Calcium