title: HPLC-MS-based methods for the study of metabonomics.
abstract: The development and use of HPLC-MS for the study of metabonomics is reviewed. To date the technique has been applied to the analysis of urine samples obtained from studies in rodents in investigations of physiological variation (e.g., factors such as strain, gender, diurnal variation, etc.) and toxicity. Examples are provided of the use of conventional HPLC, capillary methods and the recently introduced high-resolution systems based on a combination of high pressure and small particle size ("UPLC"). Comparison is also made of the use of 1H NMR spectroscopy and HPLC-MS for the analysis of biofluid samples and the advantages and limitations of the two approaches are assessed. Likely future developments are considered.
authors.affiliation: Department of Drug Metabolism and Pharmacokinetics, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK104TG, UK. Ian.Wilson@astrazeneca.com
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
authors.forename: Robert
authors.initials: R
authors.lastname: Plumb
authors.forename: Jennifer
authors.initials: J
authors.lastname: Granger
authors.forename: Hilary
authors.initials: H
authors.lastname: Major
authors.forename: Rebecca
authors.initials: R
authors.lastname: Williams
authors.forename: Eva M
authors.initials: EM
authors.lastname: Lenz
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D014018:Tissue Distribution