title: Oestrogen-modulated gene expression in the human endometrium.
abstract: To identify key regulatory mechanisms in the growth and development of the human endometrium, microarray analysis was performed on uncultured human endometrium collected during menstruation (M) and the late-proliferative (LATE-P)-phase of the menstrual cycle, as well as after 24 h incubation in the presence of oestradiol (17beta-E2). We demonstrate the expression of novel gene transcripts in the human endometrium. i.e. mucin-9, novel oestrogen-responsive gene transcripts, i.e. gelsolin and flotillin-1, and genes known to be expressed in human endometrium but not yet shown to be oestrogen responsive, i.e. connexin-37 and TFF1/pS2. Genes reported to be expressed during the implantation window and implicated in progesterone action, i.e. secretoglobin family 2A, member 2 (mammaglobin) and homeobox-containing proteins, were up-regulated in uncultured LATE-P-phase endometrium compared to M-phase endometrium. Some gene transcripts are regulated directly by 17beta-E2 alone, others are influenced by the in vivo environment as well. These observations emphasise that the regulation of endometrium maturation by oestrogen entails more then just stimulation of cell proliferation.
authors.affiliation: Research Institute GROW, Postbus 5800, 6202, AZ Maastricht, The Netherlands. Chamindiem@yahoo.co.uk
authors.forename: C
authors.initials: C
authors.lastname: Punyadeera
authors.forename: H
authors.initials: H
authors.lastname: Dassen
authors.forename: J
authors.initials: J
authors.lastname: Klomp
authors.forename: G
authors.initials: G
authors.lastname: Dunselman
authors.forename: R
authors.initials: R
authors.lastname: Kamps
authors.forename: F
authors.initials: F
authors.lastname: Dijcks
authors.forename: A
authors.initials: A
authors.lastname: Ederveen
authors.forename: A
authors.initials: A
authors.lastname: de Goeij
authors.forename: P
authors.initials: P
authors.lastname: Groothuis
mesh_terms: D000328:Adult; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D018260:Gelsolin / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008597:Menstrual Cycle / Q000502:physiology*; D046228:Microarray Analysis; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D018260:Gelsolin; D006023:Glycoproteins; D008565:Membrane Proteins; C091468:OVGP1 protein, human; C106354:flotillins; D004958:Estradiol