Micellar electrokinetic chromatography for determination of drug partition in phospholipids.
The lipophilicity of pipemidic, nalidixic and oxolinic acids was determined by forming phospholipidic micelles directly in an electrophoretic capillary. Phosphatidylcholine derivatives, namely L-alpha-dilauroyl phosphatidylcholine (DLPC) or L-alpha-dimiristoyl phosphatidylcholine (DMPC), were added in the run buffer (50 mM phosphate buffer at pH 7.4). To obtain a mixed micelle, phospholipidic derivatives and sodium cholate were together added in the run buffer. Considering the increasing of migration time when phosphatidylcholine derivative is added in the run buffer, Ks can be determined and then quinolones lipophilicity.
Quaglia
M G
MG
Dipartimento Studi Farmaceutici, Università degli Studi La Sapienza di Roma, P.le A. Moro 5-00185 Roma, Italy. mariagiovanna.quaglia@uniroma1.it
Fanali
S
S
Barbato
F
F
Donati
E
E
D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D009268:Nalidixic Acid / Q000032:analysis / Q000737:chemistry*; D010093:Oxolinic Acid / Q000032:analysis / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry*; D010876:Pipemidic Acid / Q000032:analysis / Q000737:chemistry*
D000890:Anti-Infective Agents; D010743:Phospholipids; D009268:Nalidixic Acid; D010093:Oxolinic Acid; D010876:Pipemidic Acid