title: In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.
abstract: DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular domain of DivIB was found to associate with a heterodimer of those of DivIC and FtsL. The heterodimerization of DivIC and FtsL was artificially constrained by fusion with interacting coiled-coils. Immunofluorescence experiments showed that DivIC is always localized at mid-cell, in contrast to DivIB and FtsL, which are co-localized with DivIC only during septation. Taken together, our data suggest that assembly of the trimeric complex DivIB/DivIC/FtsL is regulated during the cell cycle through controlled formation of the DivIC/FtsL heterodimer.
authors.affiliation: Laboratoire d'Ingénierie des Macromolécules, Institut de Biologie Structurale (CEA/CNRS UMR 5075/UJF), 41 rue Jules Horowitz, 38027 Grenoble, France.
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Noirclerc-Savoye
authors.forename: Audrey
authors.initials: A
authors.lastname: Le Gouëllec
authors.forename: Cécile
authors.initials: C
authors.lastname: Morlot
authors.forename: Otto
authors.initials: O
authors.lastname: Dideberg
authors.forename: Thierry
authors.initials: T
authors.lastname: Vernet
authors.forename: André
authors.initials: A
authors.lastname: Zapun
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D019281:Dimerization; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013296:Streptococcus pneumoniae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C085861:DivIB protein, Bacillus subtilis; C110609:DivIC protein, Bacillus subtilis; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C078387:ftsL protein, E coli