Development of Q-chromosome-specific DNA markers in tobacco and their use for identification of a tobacco monosomic line.
We developed seven Q-chromosome-specific DNA markers in Nicotiana tabacum by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis using two hybrid lines, and we were able to identify tobacco monosomic plants among F1 progeny derived from the cross N. tabacum Haplo-QxN. tabacum cv. Samsun NN using Q-chromosome-specific DNA markers. Based on the results, we discuss the roles of the Q chromosome in embryo sac development and embryogenesis. Here, we propose a new method for identifying DNA markers for a particular chromosome in the genus Nicotiana.
Tezuka
Takahiro
T
School of Agriculture, Ibaraki University, Ami, Ibaraki, 300-0393 Japan.
Onosato
Katsura
K
Hijishita
Saori
S
Marubashi
Wataru
W
D002678:Chimera / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D009006:Monosomy / Q000235:genetics*; D012639:Seeds / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development*
D005819:Genetic Markers