Expression of interleukin-8 gene in radical prostatectomy specimens is associated with advanced pathologic stage.
BACKGROUND
We previously reported that relative expression of E-cadherin, matrix metalloproteinases (MMPs)-2 and -9, and vascular endothelial growth factor (VEGF)/vascular permeability factor in radical prostatectomy specimens (RP) can distinguish organ-confined cancers from advanced prostate cancers. Here, we evaluate the expression of interleukin-8 (IL-8) and basic fibroblast growth factor (bFGF), two other genes involved in angiogenesis and metastasis, in RP specimens.


METHODS
The expression level of IL-8 and bFGF mRNA in the invasive edge of 41 prostate cancers of different stages was determined using a rapid colorimetric in situ hybridization (ISH) technique. Gene expression levels of IL-8 and bFGF were correlated with the Gleason score and pathologic stage to ascertain their relationship to prostate cancer progression.


RESULTS
The expression of IL-8 and bFGF genes was detected by ISH in histologically normal prostate gland epithelium as well as in glands with foci of cancer. Increased mRNA expression of IL-8 was associated with both the Gleason score and pathologic stage of tumors and distinguished organ-confined from non-confined tumors (P = 0.002). In contrast, the expression of bFGF mRNA did not correlate with the Gleason score or pathologic stage.


CONCLUSIONS
Overexpression of Il-8 mRNA, but not bFGF mRNA, in RP specimens is directly associated with progression of prostate cancer.
Uehara
Hisanori
H
Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
Troncoso
Patricia
P
Johnston
Dennis
D
Bucana
Corazon D
CD
Dinney
Colin
C
Dong
Zhongyun
Z
Fidler
Isaiah J
IJ
Pettaway
Curtis A
CA
D014408:Biomarkers, Tumor; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D011379:Prognosis; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000503:physiopathology* / Q000601:surgery; D012333:RNA, Messenger / Q000032:analysis
D014408:Biomarkers, Tumor; D016209:Interleukin-8; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2