Effects of dietary supplements of folic acid and rumen-protected methionine on lactational performance and folate metabolism of dairy cows.
The present experiment was undertaken to determine the interactions between dietary supplements of folic acid and rumen-protected methionine on lactational performance and on indicators of folate metabolism during one lactation. Fifty-four multiparous Holstein cows were assigned to 9 blocks of 6 cows each according to their previous milk production. Within each block, 3 cows were fed a diet calculated to supply methionine as 1.75% metabolizable protein, equivalent to 70% of methionine requirement, whereas the 3 other cows were fed the same diet supplemented with 18 g of a rumen-protected methionine supplement. Within each diet, the cows received 0, 3, or 6 mg/d of folic acid per kg of body weight. Rumen-protected methionine increased milk total solid concentration but not yield. Supplementary folic acid increased crude protein and casein concentrations in milk of cows fed no supplementary methionine and the effect increased as lactation progressed; it also decreased milk lactose concentration. Folic acid supplements had the opposite effects on milk crude protein, casein, and lactose concentrations in cows fed rumen-protected methionine. Milk and milk component yields and dry matter intake were unchanged. Folic acid supplementation increased serum folates and this response was greater at 8 wk of lactation. It decreased serum cysteine in cows fed rumen-protected methionine, whereas it had no effect in cows fed no supplementary methionine. The highest serum concentrations of cysteine but the lowest of vitamin B(12) were observed at 8 wk of lactation. Serum clearance of folic acid following an i.v. injection of folic acid was slower at 8 wk of lactation. During this period, the high concentrations of serum folates and cysteine, the low serum concentrations of vitamin B(12) and methionine, and the slow serum clearance of folates strongly suggest that the vitamin B(12) supply was inadequate and interfered with folate use. It could explain the limited lactational response to supplementary folic acid observed in the present experiment.
Girard
C L
CL
Agriculture and Agri-Food Canada, Dairy and Swine Research and Development Centre, Lennoxville, QC, Canada J1M 1Z3. girardch@agr.gc.ca
Lapierre
H
H
Matte
J J
JJ
Lobley
G E
GE
D000818:Animals; D002417:Cattle / Q000502:physiology*; D003545:Cysteine / Q000097:blood; D004032:Diet; D019587:Dietary Supplements; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics*; D006454:Hemoglobins / Q000032:analysis; D006710:Homocysteine / Q000097:blood; D007700:Kinetics; D007774:Lactation / Q000502:physiology*; D008715:Methionine / Q000008:administration & dosage* / Q000097:blood; D008892:Milk / Q000737:chemistry; D012417:Rumen / Q000378:metabolism*; D014805:Vitamin B 12 / Q000097:blood
D006454:Hemoglobins; D006710:Homocysteine; D005492:Folic Acid; D008715:Methionine; D003545:Cysteine; D014805:Vitamin B 12