Similarity between the association factor of ribosomal subunits and the protein Stm1p from Saccharomyces cerevisiae.
A ribosome association factor (AF) was isolated from the yeast Sacchharomyces cerevisiae. Partial amino acid sequence of AF was determined from its fragment of 25 kDa isolated by treating AF with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-Bromoindolenine (BNPS-skatole). This sequence has a 86% identity to the product of the single-copy S. cerevisiae STM1 gene that is apparently involved in several events like binding to quadruplex and triplex nucleic acids and participating in apoptosis, stability of telomere structures, cell cycle, and ribosomal function. Here we show that AF and Stm1p share some characteristics: both bind to quadruplex and Pu triplex DNA, associates ribosomal subunits, and are thermostable. These observations suggest that these polypeptides belong to a family of proteins that may have roles in the translation process.
Correia
Heriberto
H
Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad de Carabobo, Núcleo Aragua, Maracay, Aragua, Venezuela.
Medina
Rafael
R
Hernández
Alexandra
A
Bustamante
Ekaterina
E
Chakraburtty
Kalpana
K
Herrera
Flor
F
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*; D020539:Sequence Analysis, Protein
D008565:Membrane Proteins; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D029702:Schizosaccharomyces pombe Proteins; C439675:Stm1 protein, S pombe