Identification and expression of a conserved ubiquitin gene homologue of Spodoptera litura nucleopolyhedrovirus (spltNPV-I).
An ORF having a potential to code for a polypeptide of 79 amino acids has been identified within 993 nt sequence of 2 kb EcoRI-W fragment of Spodoptera litura nucleopolyhedrovirus (SpltNPV-I). Nucleotide and deduced amino acid sequence analyses showed its identity with the ubiquitin homologue of eukaryotes (79-80%), Melanoplus sanguinipes entomopoxvirus (76%) and other baculoviruses (72-89%). The ORF is under baculovirus late promoter motif RTAAG but unlike other baculoviruses, three such motifs at -6, -10 and -27 position are present in SpltNPV. The ORF expresses as a 10 kDa proteinin E. coli and the purified recombinant protein showed crossreactivity with the rabbit anti-ubiquitin antibodies.
Jain
Monika
M
Institute of Genomics and Integrative Biology (CSIR), Delhi University Campus, Delhi- 110 007, India.
Das
Rakha Hari
RH
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D017124:Conserved Sequence / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018411:Spodoptera / Q000821:virology*; D025801:Ubiquitin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D025801:Ubiquitin