Mode of action of cellulases on dyed cotton with a reactive dye.
Cotton woven fabrics which were previously dyed with a reactive dye were treated with a commercial cellulase preparation. Dyeing with a reactive dye for cotton apparently inhibited the weight loss activity and saccharification activity of cellulase. In addition, dyed cotton was treated with highly purified cellulases which were exo-type cellulases (Cellobiohydrolase I (CBH I) and Cellobiohydrolase II (CBH II)) and endo-type cellulase (Endoglucanase II (EG II)). Exo-type cellulases were inhibited more than endo-type cellulase by dyeing in the case of saccharification activity. CBH I was severely inhibited by dyeing as compared with CBH II or EG II from the viewpoint of morphological changes in the fiber surface. Dyes on the cellulose substrates severely influenced CBH I in spite of the rare modification, because CBH I hydrolyzed cellulose with true-processive action. The change in the activity of each cellulase component on dyed cotton can affect the synergistic action of cellulases.
Yamada
Minoru
M
Techno Center, Tokai Senko K.K., Aichi, Japan.
Amano
Yoshihiko
Y
Horikawa
Eisuke
E
Nozaki
Kouichi
K
Kanda
Takahisa
T
D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry* / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry*; D032561:Cotton Fiber; D013783:Textile Industry / Q000379:methods
D004396:Coloring Agents; C056373:endoglucanase 2; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase