LCC15-MB cells are MDA-MB-435: a review of misidentified breast and prostate cell lines.
Current stocks of the LCC15-MB cell line, which we originally isolated from a human breast-bone metastasis, were found to be genetically matched to the MDA-MB-435 cell line from the Lombardi Cancer Center (MDA-MB-435-LCC) using comparative genomic hybridisation, DNA microsatellite analysis and chromosomal number. LCC15-MB stocks used for our previously published studies as well as the earliest available LCC15-MB cells also showed identity to MDA-MB-435-LCC cells. The original karyotype reported for LCC15-MB cells was considerably different to that of MDA-MB-435 cells, indicating that the original LCC15-MB cells were lost to contamination by MDA-MB-435-LCC cells. Chromosome number is the simplest test to distinguish original LCC15-MB cells (n approximately 75) from MDA-MB-435 (n approximately 52). Collectively, our results prove that LCC15-MB cells currently available are MDA-MB-435 cells and we suggest their re-designation as MDA-MB-435-LCC15 cells. We also review the known misclassification of breast and prostate cancer cell lines to date and have initiated a register maintained at http://www.svi.edu.au/cell_lines_registry.doc.
Thompson
Erik W
EW
Invasion and Metastasis Unit, University of Melbourne, Department of Surgery, St. Vincent's Hospital, 29 Regent St., Fitzroy, 3065, Australia. rik@medstv.unimelb.edu.au
Waltham
Mark
M
Ramus
Susan J
SJ
Hutchins
Anne-Marie
AM
Armes
Jane E
JE
Campbell
Ian G
IG
Williams
Elizabeth D
ED
Thompson
Phillip R
PR
Rae
James M
JM
Johnson
Michael D
MD
Clarke
Robert
R
D001943:Breast Neoplasms / Q000145:classification* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D009693:Nucleic Acid Hybridization; D011003:Ploidies; D011471:Prostatic Neoplasms / Q000145:classification* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology