title: Neurite extension in central neurons: a novel role for the receptor tyrosine kinases Ror1 and Ror2.
abstract: Neurite elongation and branching are key cellular events during brain development as they underlie the formation of a properly wired neuronal network. Here we report that the receptor tyrosine kinases Ror1 and Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons. Upon Ror1 or Ror2 suppression using antisense oligonucleotides or RNA interference (RNAi), neurons extended shorter and less branched minor processes when compared to those in control cells. In addition, Ror-depleted cells elongated longer, albeit less branched, axons than seen in control cells. Conversely, Ror overexpression both in non-neuronal cells and in hippocampal neurons resulted in the enhanced extension of short and highly branched processes. These phenotypes were accompanied by changes in the microtubule-associated proteins MAP1B and MAP2. Taken together, these results support a novel role for Ror receptors as modulators of neurite extension in central neurons.
authors.affiliation: Department of Cell and Molecular Biology, Feinberg School of Medicine and Institute for Neuroscience, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Paganoni
authors.forename: Adriana
authors.initials: A
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010641:Phenotype; D034622:RNA Interference / Q000502:physiology; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D016376:Oligonucleotides, Antisense; D011956:Receptors, Cell Surface; C085284:microtubule-associated protein 1B; C078716:ROR1 protein, human; C078717:ROR2 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; C477011:Ror1 protein, mouse; C477010:Ror2 protein, mouse