title: The use of an hydrogen peroxide multipurpose isolator for inhouse preparation of human embryo culture media: a unique successful Swiss randomized prospective study.
abstract: OBJECTIVE
  To develop inhouse made (IHM) embryo culture medium with a Multipurpose Isolator and compare the embryo development in a prospective randomized study with commercial media.
  
  
  METHODS
  Fertilization by intracytoplasmic single sperm injection (ICSI) of Metaphase II oocytes obtained after 96 controlled ovarian hyperstimulation cycles in patients not older than 37 years. Transfer of zygotes to IHM or commercial Cook Sydney IVF Cleavage medium (SIC) immediately after pronucleus observation. Evaluation of embryo cleavage and score, pregnancy, and implantation rate.
  
  
  RESULTS
  From 100 zygotes cultured in SIC, 61% were at the 4 cell stage 45 h after ICSI compared to 77% (78/101) in the IHM, P < 0.05. The mean embryo score with IHM was 3.9 +/- 0.9 compared to 3.5 +/- 1.2 with SIC, P < 0.05. The clinical pregnancy rate per transfer was 38.9% (37/95), the implantation rate was 23% (46/200), and no differences were observed between the groups.
authors.affiliation: Fertility Laboratory, Frauenklinik, Kantonsspital Baden, Switzerland. marc.vandenbergh@ksb.ch
authors.forename: Marc J G
authors.initials: MJ
authors.lastname: Van den Bergh
authors.forename: Antonino
authors.initials: A
authors.lastname: Siragusa
authors.forename: André
authors.initials: A
authors.lastname: Dubied
authors.forename: Priska
authors.initials: P
authors.lastname: Vonbach
authors.forename: Mary Fahy
authors.initials: MF
authors.lastname: Deshe
authors.forename: Klara
authors.initials: K
authors.lastname: Flügel
authors.forename: Sandra
authors.initials: S
authors.lastname: Ruflin
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Stutz
authors.forename: Kirsten
authors.initials: K
authors.lastname: Teufelberger
authors.forename: Anette
authors.initials: A
authors.lastname: Kratzer
authors.forename: Cornelia
authors.initials: C
authors.lastname: Urech
authors.forename: Michael
authors.initials: M
authors.lastname: Hohl
mesh_terms: D000328:Adult; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D003470:Culture Media / Q000592:standards*; D004203:Disinfection / Q000379:methods; D010064:Embryo Implantation / Q000187:drug effects; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D006861:Hydrogen Peroxide / Q000627:therapeutic use*; D007185:Incubators; D008297:Male; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013557:Switzerland
chemical_list: D000891:Anti-Infective Agents, Local; D003470:Culture Media; D006861:Hydrogen Peroxide