Frequency of factor V leiden mutation.
OBJECTIVE
To determine the frequency of factor V leiden mutation.


METHODS
Observational study.


METHODS
One-year, January 2001 to December 2001 at the Armed Forces Institute of Pathology, Rawalpindi, Pakistan.


METHODS
Two hundred subjects each of apparently healthy and unrelated Punjabi and Pathan origins were included in the study. Peripheral blood samples were collected in EDTA and DNA extracted by phenol-chloroform extraction method. DNA analysis was done by PCR for restriction fragment length polymorphism. The product was digested overnight with Mn/1 and electrophoresed on acrylamide gel to detect 67 and 153 base pair fragments of factor V leiden against 37, 67 and 116 base pair fragments of normal factor V.


RESULTS
In the 400 subjects studied, only 5 cases of heterozygotes for factor V leiden were detected. The overall carrier rate was 1.3% (95% Cl 0.2-2.2%). The carrier rate in Punjabis and Pathans was 1% and 1.5% respectively.


CONCLUSIONS
This study confirms that the prevalence of factor V leiden is low in Asians and Africans as compared to the European population.
Nasiruddin
Department of Pathology, 136 Field Ambulance.
Zahur-ur-Rehman
Anwar
Masood
M
Ahmed
Suhaib
S
Ayyub
Muhammad
M
Ali
Waqar
W
D000328:Adult; D005165:Factor V / Q000235:genetics*; D005260:Female; D005820:Genetic Testing; D006801:Humans; D008297:Male; D010154:Pakistan / Q000453:epidemiology; D010865:Pilot Projects; D017354:Point Mutation; D015995:Prevalence; D019851:Thrombophilia / Q000453:epidemiology / Q000235:genetics
C095381:factor V Leiden; D005165:Factor V