title: Interference of dextran in biuret-type assays of serum proteins.
abstract: Dextran interference in biuret-type assays of total serum proteins was investigated in a Belgian National External Quality Assurance Survey with 256 participants. In vitro supplementation of therapeutic (10% Gentran 70) dextran concentrations showed a broadly varying (from 0 to 20%) negative interference. The analytical interference was found to depend on both the sodium hydroxide and tartrate concentrations in the reagent formulation. The dry chemistry biuret method was not affected by the dextran interference. In a number of cases, the effects observed may be of clinical importance. Both clinicians and laboratory staff should be aware of the persistence of this analytical problem.
authors.affiliation: Department of Clinical Chemistry, University Hospital Ghent, Ghent, Belgium. joris.delanghe@ugent.be
authors.forename: Joris R
authors.initials: JR
authors.lastname: Delanghe
authors.forename: Nicole
authors.initials: N
authors.lastname: Hamers
authors.forename: Youri E
authors.initials: YE
authors.lastname: Taes
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Libeer
mesh_terms: D001738:Biuret Reaction / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D019963:Clinical Chemistry Tests; D003911:Dextrans / Q000737:chemistry*; D006801:Humans; D010952:Plasma Substitutes / Q000737:chemistry; D011933:Reagent Kits, Diagnostic; D012044:Regression Analysis
chemical_list: D001798:Blood Proteins; D003911:Dextrans; D010952:Plasma Substitutes; D011933:Reagent Kits, Diagnostic