title: Suaveolindole, a new mass-limited antibacterial indolosesquiterpene from Greenwayodendron suaveolens obtained via high-throughput natural products chemistry methods.
abstract: Utilizing high-throughput isolation, purification, and analysis methods applied to a natural products library, a new mass-limited antibacterial indolosesquiterpene, suaveolindole (1), was obtained from Greenwayodendron suaveolens. The miniaturization of the structure elucidation of 1 was performed primarily using the CapNMR probe. Compound 1 was found to possess significant in vitro antibacterial activity against the Gram-positive bacteria Bacillus subtilis (ATCC 43223), Staphylococcus aureus (ATTC 6538P), and methicillin-resistant Staphylococcus aureus (ATTC 33591), with MIC values of 4, 8, and 8 microg/mL, respectively.
authors.affiliation: Lead Discovery and Rapid Structure Elucidation Group, Sequoia Sciences, Inc., San Diego, California 92121, USA.
authors.forename: Hye-Dong
authors.initials: HD
authors.lastname: Yoo
authors.forename: Peadar A
authors.initials: PA
authors.lastname: Cremin
authors.forename: Lu
authors.initials: L
authors.lastname: Zeng
authors.forename: Eliane
authors.initials: E
authors.lastname: Garo
authors.forename: Caroline T
authors.initials: CT
authors.lastname: Williams
authors.forename: Chris M
authors.initials: CM
authors.lastname: Lee
authors.forename: Matt G
authors.initials: MG
authors.lastname: Goering
authors.forename: Mark
authors.initials: M
authors.lastname: O'Neil-Johnson
authors.forename: Gary R
authors.initials: GR
authors.lastname: Eldridge
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Hu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D005681:Gabon; D007711:Klebsiella pneumoniae / Q000187:drug effects; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D012717:Sesquiterpenes; C498139:suaveolindole