Use of an alternative Archaea-specific probe for methanogen detection.
An alternative 16S rRNA-targeted oligonucleotide probe specific for Archaea was developed and used for detection of methanogens in anaerobic reactors. The designed probe was checked for its specificity by computer-aided comparative sequence analysis. For in situ application, optimal stringency conditions were adjusted by performing whole cell hybridization using target and nontarget organisms. Anaerobic sludge samples were examined by in situ hybridization for methanogenic populations. The relative abundance of methanogens was monitored with epifluorescence microscopy. Individual cells could be visualized with strong fluorescence signals after hybridization with the newly developed probe.
Jupraputtasri
Wiwat
W
School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand.
Boonapatcharoen
Nimaradee
N
Cheevadhanarak
Supapon
S
Chaiprasert
Pawinee
P
Tanticharoen
Morakot
M
Techkarnjanaruk
Somkiet
S
D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D019605:Euryarchaeota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D012722:Sewage / Q000382:microbiology
D015342:DNA Probes; D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S; D012722:Sewage