title: Nucleosomal structure of undamaged DNA regions suppresses the non-specific DNA binding of the XPC complex.
abstract: The XPC protein complex is a DNA damage detector of human nucleotide excision repair (NER). Although the XPC complex specifically binds to certain damaged sites, it also binds to undamaged DNA in a non-specific manner. The addition of a large excess of undamaged naked DNA competitively inhibited the specific binding of the XPC complex to (6-4) photoproducts and the NER dual incision step in cell-free extracts. In contrast, the addition of undamaged nucleosomal DNA as a competitor suppressed both of these inhibitory effects. Although nucleosomes positioned on the damaged site inhibited the binding of the XPC complex, the presence of nucleosomes in undamaged DNA regions may help specific binding of the XPC complex to damaged sites by excluding its non-specific binding to undamaged DNA regions.
authors.affiliation: Cellular Physiology Laboratory, Discovery Research Institute, RIKEN, Saitama 351-0198, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasuda
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sugasawa
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Shigenori
authors.initials: S
authors.lastname: Iwai
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Shiomi
authors.forename: Fumio
authors.initials: F
authors.lastname: Hanaoka
mesh_terms: D001483:Base Sequence; D002457:Cell Extracts; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D018983:DNA Footprinting; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D004268:DNA-Binding Proteins; D009707:Nucleosomes; D011994:Recombinant Proteins; C086984:XPC protein, human; D004247:DNA