title: [Gene chip analysis of mutation of HBV-DNA open reading frame].
abstract: OBJECTIVE
  To detect the mutation of HBV-DNA open reading frame (ORF), provide evidences for clinical diagnosis and treatment.
  
  
  METHODS
  By using gene chip technique, HBV-DNA was amplified by PCR, incorporated with fluorescence, hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF.
  
  
  RESULTS
  The mutations of HBV-DNA ORF existed widespread. The rates of mutation on Pre-C 1896, PreC 1814, BCP 1762, BCP 1764, P 528, P 552MI, P 552MV were 23.5%, 3.9%, 55.9%, 53.9%,39.2%, 38.2%, 10.8%, respectively.
  
  
  CONCLUSIONS
  The gene chip technique possesses extremely high sensitivity and reliability,it is one of the effective methods to detect gene mutation. The mutation of HBV-DNA has important influence on the stability and progress of the disease, and on the judgement of prognosis.
authors.affiliation: Jinan Infectious Disease Hospital, Jinan 250021, China.
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Chen
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Du
authors.forename: Yan-qin
authors.initials: YQ
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: An
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D006512:Hepatitis B Core Antigens / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016366:Open Reading Frames / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens