Macromolecular oxidation in anisotonic suspensions of mouse spleen cells.
Macromolecular oxidative alterations have been analysed in vitro in anisotonic suspensions of mouse splenocytes. Both hypertonicity and hypotonicity induced the generation of thiobarbituric acid reactive species (TBARS) and carbonylation of the proteins, which took place along with cell death. Addition of antioxidants partially inhibited oxidative changes in isotonic and hypotonic suspensions. Anisotonic shock of mouse splenocytes proved to be an inducer of oxidative stress. The oxidative macromolecular alterations might contribute to pathogenesis of cell death caused by osmotic stress.
Bilić
Ivan
I
Department of Pathophysiology, Medical School, University of Zagreb, Zagreb, Croatia. ibilic@mef.hr
Kovac
Zdenko
Z
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D006982:Hypertonic Solutions / Q000494:pharmacology*; D007038:Hypotonic Solutions / Q000494:pharmacology*; D066298:In Vitro Techniques; D046911:Macromolecular Substances / Q000032:analysis; D051379:Mice; D008808:Mice, Inbred CBA; D009997:Osmotic Pressure / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects / Q000502:physiology; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*
D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D046911:Macromolecular Substances; D017392:Thiobarbituric Acid Reactive Substances