title: Enthalpy changes associated with protein binding to thin films.
abstract: Molecularly imprinted thin films consisting of proteins embedded in polymerised aminophenyl boronic acid have been made on glass supports. The protein contents of the films have been optimised to achieve a maximum energy of interaction between the film and the native template. The fabrication of the films and the subsequent removal from their surfaces of the imprint proteins has been shown to be a facile and easily reproduced process. The enthalpy changes associated with the rebinding of the films with their original templates (lysozyme and cytochrome c) and with non-native templates has been examined by micro-calorimetry. The results demonstrate that thin films can be successfully imprinted as shown by the significant reduction in the enthalpy (DeltaH) observed when the films were rebound with proteins other than the original templates. Additionally, it was shown that after binding, non-template proteins could be removed by washing and a greater enthalpy again observed when the films were rebound with the native protein compared to that which had been found with the non-native protein.
authors.affiliation: Chemical Engineering Department, National Cheng Kung University, Tainan 70101, Taiwan.
authors.forename: John
authors.initials: J
authors.lastname: Rick
authors.forename: Tse-Chuan
authors.initials: TC
authors.lastname: Chou
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry* / Q000648:ultrastructure*; D004735:Energy Transfer; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D009113:Muramidase / Q000737:chemistry* / Q000648:ultrastructure*; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D045304:Cytochromes c; D009113:Muramidase