Oxidative stress related markers in the "VITA" and the centenarian projects.
Oxidative stress seems to play an important role in the pathophysiology of Alzheimer's disease (AD). At present there are no easily accessible biochemical markers for AD. We performed activity assays for platelet MAO-B and erythrocyte Cu/Zn-SOD as well as Western blotting for these two proteins. Moreover, we assessed plasma lactoferrin and performed RFLP-analysis for the MAO-B-intron-13-polymorphism in patients from the Vienna-Transdanube Aging (VITA) and from the so called centenarian project. The first one, VITA, is a community-based cohort study of all 75 years old inhabitants of a geographical region of Vienna. The centenarian project investigates chronic care in-old patients suffering from AD. In both sexes platelet MAO-B activity increased significantly in the AD group, and Cu/Zn-SOD activity decreased, but the latter effect was significant only in females. No significant difference was found regarding plasma lactoferrin. No correlation was found between MAO-Bi13 and MAO-B platelet activity or allele MAO-Bi13 and disease frequency. These results point to the possibility that a combination of MAO-B and SOD activity levels might be useful tools for an early diagnosis of AD.
Grünblatt
E
E
Clinic for Psychiatry and Psychotherapy, Department Clinical Neurochemistry, Bayerische Julius-Maximilians-University, Fuchsleinstr 15, Würzburg, Germany. edna.gruenblatt@mail.uni-wuerzburg.de
Schlösser
R
R
Fischer
P
P
Fischer
M O
MO
Li
J
J
Koutsilieri
E
E
Wichart
I
I
Sterba
N
N
Rujescu
D
D
Möller
H J
HJ
Adamcyk
W
W
Dittrich
B
B
Müller
F
F
Oberegger
K
K
Gatterer
G
G
Jellinger
K J
KJ
Mostafaie
N
N
Jungwirth
S
S
Huber
K
K
Tragl
K H
KH
Danielczyk
W
W
Riederer
P
P
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000201:enzymology* / Q000453:epidemiology; D000704:Analysis of Variance; D001317:Austria / Q000453:epidemiology; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D015331:Cohort Studies; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000097:blood; D008297:Male; D008609:Mental Status Schedule / Q000706:statistics & numerical data; D008995:Monoamine Oxidase / Q000097:blood* / Q000235:genetics; D018384:Oxidative Stress / Q000502:physiology*; D010949:Plasma / Q000378:metabolism; D011110:Polymorphism, Genetic; D012044:Regression Analysis; D012737:Sex Factors; D013482:Superoxide Dismutase / Q000097:blood*
D015415:Biomarkers; D013482:Superoxide Dismutase; D008995:Monoamine Oxidase; D007781:Lactoferrin