Antioxidant effect of bovine serum albumin on membrane lipid peroxidation induced by iron chelate and superoxide.
Albumin is supposed to be the major antioxidant circulating in blood. This study examined the prevention of membrane lipid peroxidation by bovine serum albumin (BSA). Lipid peroxidation was induced by the exposing of enzymatically generated superoxide radicals to egg yolk phosphatidylcholine liposomes incorporating lipids with different charges in the presence of chelated iron catalysts. We used three kinds of Fe3+-chelates, which initiated reactions that were dependent on membrane charge: Fe3+-EDTA and Fe3+-EGTA catalyzed peroxidation in positively and negatively charged liposomes, respectively, and Fe3+-NTA, a renal carcinogen, catalyzed the reaction in liposomes of either charge. Fe3+-chelates initiated more lipid peroxidation in liposomes with increased zeta potentials, followed by an increase of their availability for the initiation of the reaction at the membrane surface. BSA inhibits lipid peroxidation by preventing the interaction of iron chelate with membranes, followed by a decrease of its availability in a charge-dependent manner depending on the iron-chelate concentration: one is accompanied and the other is unaccompanied by a change in the membrane charge. The inhibitory effect of BSA in the former at high concentrations of iron chelate would be attributed to its electrostatic binding with oppositely charged membranes. The inhibitory effect in the latter at low concentrations of iron chelate would be caused by BSA binding with iron chelates and keeping them away from membrane surface where lipid peroxidation is initiated. Although these results warrant further in vivo investigation, it was concluded that BSA inhibits membrane lipid peroxidation by decreasing the availability of iron for the initiation of membrane lipid peroxidation, in addition to trapping active oxygens and free radicals.
Fukuzawa
Kenji
K
Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi-1, Tokushima 770-8505, Japan. fukuzawa@ph.tokushima-u.ac.jp
Saitoh
Yasuaki
Y
Akai
Kaori
K
Kogure
Kentaro
K
Ueno
Satoru
S
Tokumura
Akira
A
Otagiri
Masaki
M
Shibata
Akira
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D007502:Iron Chelating Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008563:Membrane Lipids / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism* / Q000494:pharmacology
D000975:Antioxidants; D007502:Iron Chelating Agents; D008563:Membrane Lipids; D010455:Peptides; D013481:Superoxides; D012710:Serum Albumin, Bovine