title: Effects of glutamine-containing total parenteral nutrition on phagocytic activity and anabolic hormone response in rats undergoing gastrectomy.
abstract: OBJECTIVE
  To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats undergoing a gastrectomy.
  
  
  METHODS
  Rats with an internal jugular catheter were divided into 2 experimental groups and received total parenteral nutrition (TPN). The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in amino acid content. One group received conventional TPN (control), and in the other group, 25% of the total amino acid nitrogen was replaced with Gln. After receiving TPN for 3 d, one-third of the rats in each experimental group were sacrificed as the baseline group. The remaining rats underwent a partial gastrectomy and were killed 1 and 3 d, respectively, after surgery. Plasma, peritoneal lavage fluid (PLF), and urine samples were collected for further analysis.
  
  
  RESULTS
  The Gln group had fewer nitrogen losses 1 and 2 d after surgery (d1, 16.6+/-242.5 vs -233.4+/-205.9 mg/d, d2, 31.8+/-238.8 vs -253.4+/-184.6 mg/d, P<0.05). There were no differences in plasma growth hormone (GH) and insulin-like growth factor-1 levels between the 2 groups before or after surgery. The phagocytic activity of peritoneal macrophages was higher in the Gln group than in the control group 1 d after surgery (A 1185+/-931 vs 323+/-201, P<0.05). There were no differences in the phagocytic activities of blood polymorphonuclear neutrophils between the 2 groups at the baseline or on the postoperative days. No significant differences in interleukin-1beta or interleukin-6 concentrations in PLF were observed between the 2 groups. However, tumor necrosis factor-alpha level in PLF was significantly lower in the Gln group than in the control group on postoperative d 3.
  
  
  CONCLUSIONS
  TPN supplemented with Gln can improve the nitrogen balance, and enhance macrophage phagocytic activity at the site of injury. However, Gln supplementation has no effect on phagocytic cell activity in the systemic circulation, GH and insulin-like growth factor-1 might not be responsible for attenuating nitrogen losses in rats with a partial gastrectomy.
authors.affiliation: Institute of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan 110, China.
authors.forename: Chen-Hsien
authors.initials: CH
authors.lastname: Lee
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Chiu
authors.forename: Soul-Chin
authors.initials: SC
authors.lastname: Chen
authors.forename: Chih-Hsiung
authors.initials: CH
authors.lastname: Wu
authors.forename: Sung-Ling
authors.initials: SL
authors.lastname: Yeh
mesh_terms: D000818:Animals; D001835:Body Weight; D005743:Gastrectomy; D005973:Glutamine / Q000097:blood / Q000494:pharmacology*; D013006:Growth Hormone / Q000097:blood* / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000502:physiology; D009584:Nitrogen / Q000378:metabolism; D010289:Parenteral Nutrition, Total / Q000379:methods*; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005973:Glutamine; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D009584:Nitrogen