title: Metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 and its mutant.
abstract: The metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 from Bacillus megaterium and a mutant enzyme of it (AL4V; Ala74Gly, Phe87Val, Leu188Gln triple mutant) was examined. Both purified enzymes metabolized 1-monochloro-, 2,3-dichloro-, and 2,3,7-trichloro-dibenzo-p-dioxin, but not 2,3,7,8-tetrachloro-dibenzo-p-dioxin. The mutant AL4V had 2-12 times higher activity than the wild-type P450 BM-3 towards polychlorinated dibenzo-p-dioxins. The products were hydroxylated at an unsubstituted position and/or showing migration of the chloride and were less toxic derivatives with lower than 10% toxicity of the original compounds.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Woro Triarsi
authors.initials: WT
authors.lastname: Sulistyaningdyah
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Li
authors.forename: Raku
authors.initials: R
authors.lastname: Shinkyo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sakaki
authors.forename: Kuniyo
authors.initials: K
authors.lastname: Inouye
authors.forename: Rolf D
authors.initials: RD
authors.lastname: Schmid
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D001410:Bacillus megaterium / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D015202:Protein Engineering / Q000379:methods*; D012989:Soil Pollutants / Q000493:pharmacokinetics*; D013045:Species Specificity
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants; D003577:Cytochrome P-450 Enzyme System