title: Presence of neuroglobin in cultured astrocytes.
abstract: Neuroglobin (Ngb), a recently discovered intracellular respiratory globin in neurons, may play a crucial role in oxygen homeostasis in the brain. We report preliminary findings indicating the presence of functional neuroglobin in primary cultures of cerebral cortical astrocytes. Reverse transcription real-time polymerase chain reaction (RRT-PCR) and immunostaining confirmed such presence in cultured astrocytes isolated from newborn mouse brain. Ngb antisense treatment increased apoptosis in ischemic astrocytes. The discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain.
authors.affiliation: Neuroscience Research Institute, Peking University, Key Laboratory of Neuroscience, Ministry of Education, Department of Neurobiology, Peking University Health Science Center, Beijing, People's Republic of China.
authors.forename: Xiao Qian
authors.initials: XQ
authors.lastname: Chen
authors.forename: Lu Ye
authors.initials: LY
authors.lastname: Qin
authors.forename: Chen Gang
authors.initials: CG
authors.lastname: Zhang
authors.forename: Li Tao
authors.initials: LT
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Lok Ting
authors.initials: LT
authors.lastname: Lau
authors.forename: Yin-Wan Wendy
authors.initials: YW
authors.lastname: Fung
authors.forename: David A
authors.initials: DA
authors.lastname: Greenberg
authors.forename: Albert Cheung-Hoi
authors.initials: AC
authors.lastname: Yu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D019556:COS Cells; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005914:Globins / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D000078787:Neuroglobin; D010101:Oxygen Consumption / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins