title: Time course of dehydrating effects of isosorbide on experimentally induced endolymphatic hydrops in guinea pigs.
abstract: Osmotic diuretics are therapeutic agents used to reduce endolymphatic hydrops. However, glycerol-induced change in endolymph volume is followed by a rebound phenomenon. In this study, we investigated the rebound phenomenon occurring with isosorbide, an osmotic diuretic used as a therapeutic agent for Ménière's disease in Japan. Forty guinea pigs underwent surgical obliteration of the endolymphatic sac. Thirty received isosorbide orally 1 month after surgery. These animals were sacrificed 3, 6, or 12 h after isosorbide intake. The remaining 10 animals served as controls. Quantitative assessment of changes in the endolymphatic space was performed light-microscopically. Isosorbide reduced cochlear endolymph volume, with a peak reduction 6 h after intake. Thereafter, no prominent rebound phenomenon was noted. Clinically, since isosorbide is orally administered every 8 h, rebound phenomenon need not be considered in the treatment with isosorbide.
authors.affiliation: Department of Otolaryngology, Kochi Medical School, Nankoku, Japan. kakigia@kochi-ms.ac.jp
authors.forename: Akinobu
authors.initials: A
authors.lastname: Kakigi
authors.forename: Setsuko
authors.initials: S
authors.lastname: Takeda
authors.forename: Taizo
authors.initials: T
authors.lastname: Takeda
authors.forename: Shoichi
authors.initials: S
authors.lastname: Sawada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Azuma
authors.forename: Kasumi
authors.initials: K
authors.lastname: Higashiyama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yamakawa
authors.forename: Shunji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003053:Cochlear Duct / Q000187:drug effects; D004195:Disease Models, Animal; D004234:Diuretics, Osmotic / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004710:Endolymph / Q000187:drug effects* / Q000378:metabolism; D018159:Endolymphatic Hydrops / Q000139:chemically induced / Q000188:drug therapy*; D005990:Glycerol / Q000737:chemistry / Q000494:pharmacology; D006168:Guinea Pigs; D007547:Isosorbide / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012008:Recurrence; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D004234:Diuretics, Osmotic; D005990:Glycerol; D007547:Isosorbide