The effect of conjugated linoleic acid on transepithelial calcium transport and mediators of paracellular permeability in human intestinal-like Caco-2 cells.
Conjugated linoleic acid (CLA) increases paracellular permeability across human intestinal-like Caco-2 cell monolayers, which transport Ca predominantly by the transcellular route. In vivo, however, paracellular Ca transport is the predominant route of Ca transport. Therefore, the objective of this study was to investigate the effect of CLA on transepithelial Ca transport in Caco-2 cells transporting Ca predominantly by the paracellular route. Cells were seeded onto permeable transport membranes and allowed to differentiate, over 14 d, into intestinal-like cell monolayers. Monolayers (n=9/treatment) were exposed to 0 (control) or 80 microM- 18:2, -cis-9, trans-11 CLA or -trans-10, cis-12 CLA for 14 d prior to Ca transport studies. Overall transepithelial Ca transport as well as transcellular and parcellular Ca transport was significantly increased (P<0.001) by exposure of Caco-2 cells to both isomers of CLA, an effect which appeared to be related to altered localization of zona occludens 1 (a tight junction protein).
Jewell
C
C
Department of Food and Nutritional Sciences, University College, Western Road, Cork, Ireland.
Cusack
S
S
Cashman
K D
KD
D001692:Biological Transport; D018938:Caco-2 Cells; D002118:Calcium / Q000502:physiology*; D002463:Cell Membrane Permeability; D005455:Fluorescent Antibody Technique; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000494:pharmacology*; D013237:Stereoisomerism; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism
D044243:Linoleic Acids, Conjugated; D002118:Calcium