Marine fish egg hydration is aquaporin-mediated.
The positive buoyancy of marine fish eggs in sea water, allowed by hydration of the oocyte, is critical for their survival and dispersion in the ocean. We isolated an aquaporin, SaAQP1o, that belongs to a unique subfamily of aquaporin-1-like channels specifically evolved in teleosts and mainly expressed in the ovary. We further show that hormone-induced fish oocyte hydration is a highly controlled process based on the interplay between protein hydrolysis and the translocation of SaAQP1o to the plasma membrane, indicating a specialized physiological role for this aquaporin.
Fabra
Mercedes
M
Center of Aquaculture-Institut de Recerca i Tecnologia Agroalimentaries, Tarragona, Spain, and Reference Center in Aquaculture, Barcelona, Spain.
Raldúa
Demetrio
D
Power
Deborah M
DM
Deen
Peter M T
PM
Cerdà
Joan
J
D000595:Amino Acid Sequence; D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000502:physiology*; D005075:Biological Evolution; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary; D005260:Female; D005399:Fishes / Q000235:genetics / Q000502:physiology; D008627:Mercuric Chloride / Q000494:pharmacology; D008871:Microvilli / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology*; D010053:Ovary; D010539:Permeability; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021541:Sea Bream / Q000235:genetics / Q000502:physiology*; D014867:Water / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics
D020346:Aquaporins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D014867:Water; D051398:Aquaporin 1; D008627:Mercuric Chloride