Isolation and properties of a pure bacterial strain capable of fluorobenzene degradation as sole carbon and energy source.
A pure bacterial strain capable of aerobic biodegradation of fluorobenzene (FB) as the sole carbon and energy source was isolated by selective enrichment from sediments collected from a polluted site. 16S rRNA and fatty acid analyses support that strain F11 belongs to a novel genus within the alpha-2 subgroup of the Proteobacteria, possibly within a new clade related to the order Rhizobiales. In batch cultures, growth of strain F11 on FB led to stoichiometric release of fluoride ion. Maximum experimental growth rate of 0.04 h-1 was obtained at FB concentration of 0.4 mM. Growth kinetics were described by the Luong model. An inhibitory effect with increasing FB concentrations was observed, with no growth occurring at concentrations higher than 3.9 mM. Strain F11 was shown to be able to use a range of other organic compounds, including other fluorinated compounds such as 2-fluorobenzoate, 4-fluorobenzoate and 4-fluorophenol. To our knowledge, this is the first time biodegradation of FB, as the sole carbon and energy source, by a pure bacterium has been reported.
Carvalho
M F
MF
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr António Bernardino de Almeida, 4200-072 Porto, Portugal.
Ferreira Jorge
R
R
Pacheco
C C
CC
De Marco
P
P
Castro
P M L
PM
D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001565:Benzoates / Q000378:metabolism; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005459:Fluorides / Q000378:metabolism; D005464:Fluorobenzenes / Q000378:metabolism* / Q000494:pharmacology; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010636:Phenols / Q000378:metabolism; D010802:Phylogeny; D011174:Portugal; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D000900:Anti-Bacterial Agents; D001565:Benzoates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005464:Fluorobenzenes; D010636:Phenols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C106880:4-fluorophenol; C022894:2-fluorobenzoic acid; D005459:Fluorides; C022893:4-fluorobenzoic acid