TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants.
Nuclear matrix attachment regions (MARs) are thought to influence the expression of the flanking genes. TM2, a new DNA fragment isolated from tobacco, can bind with the rice nuclear matrix in vitro. In this study, we investigated the effect of TM2 on transgene expression under the control of three different promoters in stably transformed rice calli and plants. The presence of TM2 flanking the transgene increased the expression of constructs based on the constitutive CaMV 35S and maize ubiquitin gene promoters in both resistant calli and transformed plants. The GUS expression directed by the photosynthetic-tissue-specific PNZIP promoter was also increased in photosynthetic tissues of transformants. However, TM2 did not change the gene expression pattern controlled by the PNZIP promoter. The effect of TM2 in transgenic plants was stronger than that in transgenic calli based on all three promoters. Our results indicate that TM2, as a novel strong MAR, can be used to increase the transgene expression levels in the whole plant or in particular tissues of monocotyledons.
Xue
Hua
H
College of Life Sciences, Shandong Agricultural University, 61 Daizong Street, Taian, Shandong, 271018, People's Republic of China.
Yang
Yu-Tao
YT
Wu
Chang-Ai
CA
Yang
Guo-Dong
GD
Zhang
Meng-Meng
MM
Zheng
Cheng-Chao
CC
D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D015530:Nuclear Matrix; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000166:cytology / Q000235:genetics*; D014170:Transformation, Genetic; D019076:Transgenes; D025801:Ubiquitin / Q000235:genetics
D018744:DNA, Plant; D025801:Ubiquitin; D005966:Glucuronidase