Cleavage at 5-methylcytosine in DNA by photosensitized oxidation with 2-methyl-1,4-naphthoquinone tethered oligodeoxynucleotides.
Photosensitized one-electron oxidation of 5-methylcytosine in DNA by 2-methyl-1,4-naphthoquinone, attached to 5'-end of an oligodeoxynucleotide strand, produced 5-formylcytosine and led to selective DNA strand cleavage at the original 5-methylcytosine configuration. This specified photoreaction is useful for positive display of 5-methylcytosine in DNA on a sequencing gel.
Yamada
Hisatsugu
H
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
Tanabe
Kazuhito
K
Nishimoto
Sei-Ichi
S
D044503:5-Methylcytosine / Q000737:chemistry*; D001345:Autoradiography; D004249:DNA Damage; D006801:Humans; D006868:Hydrolysis; D009285:Naphthoquinones / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010084:Oxidation-Reduction; D010761:Phosphorus Radioisotopes; D010777:Photochemistry; D013329:Structure-Activity Relationship; D024483:Vitamin K 3 / Q000737:chemistry*
D009285:Naphthoquinones; D009838:Oligodeoxyribonucleotides; D010761:Phosphorus Radioisotopes; D044503:5-Methylcytosine; D024483:Vitamin K 3