title: Complete genome sequence of bacteriophage T5.
abstract: The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading frames (ORFs), 61 were annotated; these annotated ORFs are mainly enzymes involved in phage DNA replication, repair, and nucleotide metabolism. At least five endonucleases that believed to help inducing nicks in T5 genomic DNA, and a DNA ligase gene was found to be split into two separate ORFs. Analysis of T5 early promoters suggests a probable motif AAA{3, 4 T}nTTGCTT{17, 18 n}TATAATA{12, 13 W}{10 R} for strong promoters that may strengthen the step modification of host RNA polymerase, and thus control transcription of phage DNA. The distinct protein domain profile and a mosaic genome structure suggest an origin from the common genetic pool.
authors.affiliation: James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Myriam
authors.initials: M
authors.lastname: Vincent
authors.forename: Yongqiao
authors.initials: Y
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Bao
authors.forename: Huimin
authors.initials: H
authors.lastname: Kong
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
mesh_terms: D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA