Visible light-induced oxidation of unsaturated components of cutins: a significant process during the senescence of higher plants.
9-Hydroperoxy-18-hydroxyoctadec-10(trans)-enoic and 10-hydroperoxy-18-hydroxyoctadec-8(trans)-enoic acids deriving from type II (i.e. involving 1O2) photooxidation of 18-hydroxyoleic acid were detected after visible light-induced senescence experiments carried out with Petroselinum sativum and subsequent cutin depolymerisation. These results showed that in senescent plants, where the 1O2 formation rate exceeds the quenching capacity of the photoprotective system, 1O2 can migrate outside the chloroplasts and affect the unsaturated components of cutins. Significant amounts of 9,18-dihydroxyoctadec-10(trans)-enoic and 10,18-dihydroxyoctadec-8(trans)-enoic acids resulting from the reduction of these photoproducts of 18-hydroxyoleic acid were also detected in different natural samples. These results well support the significance of the photooxidation of the unsaturated components of higher plant cutins in the natural environment.
Rontani
Jean-François
JF
Laboratoire de Microbiologie Géochimie et Ecologie Marines (UMR 6117), Faculté des Scs. de Luminy, Centre d'Océanologie de Marseille (OSU), Campus de Luminy, case 901, F-13288 Marseille Cedex, France. rontani@com.univ-mrs.fr
Rabourdin
Adélaïde
A
Pinot
Franck
F
Kandel
Sylvie
S
Aubert
Claude
C
D004777:Environment; D008027:Light; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction / Q000528:radiation effects; D063245:Plant Development; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D010944:Plants / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D012996:Solutions / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D008563:Membrane Lipids; D012996:Solutions; D019301:Oleic Acid; C000521:cutin