title: Molybdate-dependent expression of the periplasmic nitrate reductase in Bradyrhizobium japonicum.
abstract: The napEDABC genes of Bradyrhizobium japonicum encode the periplasmic nitrate reductase, an Mo-containing enzyme which catalyses the reduction of nitrate to nitrite when oxygen concentrations are limiting. In this bacterium, another set of genes, modABC, code for a high affinity ABC-type Mo transport system. A B. japonicum modA mutant has been obtained that is not capable of growing anaerobically with nitrate and lacks nitrate reductase activity. Under nitrate respiring conditions, when Mo concentrations are limiting, the B. japonicum modA mutant lacked both the 90 kDa protein corresponding to the NapA component of the periplasmic nitrate reductase, and the membrane-bound 25 kDa c-type cytochrome NapC. Regulatory studies using a napE-lacZ fusion indicated that napE expression was highly reduced in the modA mutant background when the cells were incubated anaerobically with nitrate under Mo-deficient conditions.
authors.affiliation: Fachbereich Biologie der Philipps Universität Marburg, Zellbiologie und Angewandte Botanik, Karl-von-Frisch-Str. 8, 35032 Marburg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Bonnard
authors.forename: A
authors.initials: A
authors.lastname: Tresierra-Ayala
authors.forename: E J
authors.initials: EJ
authors.lastname: Bedmar
authors.forename: M J
authors.initials: MJ
authors.lastname: Delgado
mesh_terms: D015153:Blotting, Western; D020369:Bradyrhizobium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D008982:Molybdenum / Q000494:pharmacology*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism*; D019897:Periplasm / Q000187:drug effects* / Q000201:enzymology
chemical_list: C044659:molybdate; D008982:Molybdenum; D009565:Nitrate Reductases; D050901:Nitrate Reductase