title: Angiotensinogen and plasminogen activator inhibitor-1 gene polymorphism in relation to chronic allograft dysfunction.
abstract: Chronic allograft dysfunction (CAD) is the most common cause of allograft failure in the long-term, and current immunologic strategies have little effect on this condition. The renin-angiotensin system (RAS) plays important roles progression of chronic renal disease. It is thought that plasminogen activator inhibitor-1 (PAI-1) functions in the RAS, in addition to involvement in thrombotic risk and fibrosis. This study investigated possible links between angiotensinogen (AGT) genotypes (M235T/MM, MT, TT) and PAI-1 genotypes (4G4G, 4G5G, 5G5G) and CAD assessments of both types of polymorphism were performed in 82 renal allograft recipients. One hundred healthy subjects were also investigated for AGT polymorphism, and 80 healthy subjects for PAI-1 polymorphism. Genotypes were determined using polymerase chain reaction (PCR) sequence-specific primers, and PCR followed by restriction fragment length polymorphism analysis. Kidney recipients with CAD had significantly lower frequencies of the MM genotype and the M allele than the recipients without CAD (p < 0.05 and <0.001). The transplant recipients with CAD also had significantly lower frequencies of the 5G5G genotype and the 5G allele than those without CAD (p < 0.001 and <0.05). Determination of AGT M235T and PAI-1 genotypes prior to transplantation may help identify patients who at risk for chronic renal transplant dysfunction.
authors.affiliation: Department of Nephrology, Gazi University, Ankara, Turkey. akadriyer@hotmail.com
authors.forename: Kadriye
authors.initials: K
authors.lastname: Reis
authors.forename: Turgay
authors.initials: T
authors.lastname: Arinsoy
authors.forename: Ulver
authors.initials: U
authors.lastname: Derici
authors.forename: Sevim
authors.initials: S
authors.lastname: Gonen
authors.forename: Zerrin
authors.initials: Z
authors.lastname: Bicik
authors.forename: Oguz
authors.initials: O
authors.lastname: Soylemezoglu
authors.forename: Unal
authors.initials: U
authors.lastname: Yasavul
authors.forename: Enver
authors.initials: E
authors.lastname: Hasanoglu
authors.forename: Sukru
authors.initials: S
authors.lastname: Sindel
mesh_terms: D000328:Adult; D000808:Angiotensinogen / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000235:genetics*; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011110:Polymorphism, Genetic; D012084:Renin-Angiotensin System / Q000502:physiology
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D000808:Angiotensinogen