title: Multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) to identify recurrent chromosomal alterations in breast tumour cell lines.
abstract: Recurrent chromosome breakpoints in tumour cells may point to cancer genes, but not many have been molecularly characterised. We have used multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) on breast tumour cell lines to identify regions of chromosome break created by inversions, duplications, insertions and translocations on chromosomes 1, 5, 8, 12 and 17. We delineate a total of 136 regions of break, some of them occurring with high frequency. We further describe two examples of dual-colour FISH characterisation of breakpoints, which target the 1p36 and 5p11-12 regions. Both breaks involve genes whose function is unknown to date. The mbanding-FISH strategy constitutes an efficient first step in the search for potential cancer genes.
authors.affiliation: Laboratory of Molecular Cytogenetics, Department of Molecular Oncology, Paoli-Calmettes Institute-UMR599 INSERM, Marseille Cancer Research Institute, Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Letessier
authors.forename: M-J
authors.initials: MJ
authors.lastname: Mozziconacci
authors.forename: A
authors.initials: A
authors.lastname: Murati
authors.forename: J
authors.initials: J
authors.lastname: Juriens
authors.forename: J
authors.initials: J
authors.lastname: Adélaïde
authors.forename: D
authors.initials: D
authors.lastname: Birnbaum
authors.forename: M
authors.initials: M
authors.lastname: Chaffanet
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping