title: A novel scaffold protein, TANC, possibly a rat homolog of Drosophila rolling pebbles (rols), forms a multiprotein complex with various postsynaptic density proteins.
abstract: We cloned from the rat brain a novel gene, tanc (GenBank Accession No. AB098072), which encoded a protein containing three tetratricopeptide repeats (TPRs), ten ankyrin repeats and a coiled-coil region, and is possibly a rat homolog of Drosophila rolling pebbles (rols). The tanc gene was expressed widely in the adult rat brain. Subcellular distribution, immunohistochemical study of the brain and immunocytochemical studies of cultured neuronal cells indicated the postsynaptic localization of TANC protein of 200 kDa. Pull-down experiments showed that TANC protein bound PSD-95, SAP97, and Homer via its C-terminal PDZ-binding motif, -ESNV, and fodrin via both its ankyrin repeats and the TPRs together with the coiled-coil domain. TANC also bound the alpha subunit of Ca2+/calmodulin-dependent protein kinase II. An immunoprecipitation study showed TANC association with various postsynaptic proteins, including guanylate kinase-associated protein (GKAP), alpha-internexin, and N-methyl-D-aspartate (NMDA)-type glutamate receptor 2B and AMPA-type glutamate receptor (GluR1) subunits. These results suggest that TANC protein may work as a postsynaptic scaffold component by forming a multiprotein complex with various postsynaptic density proteins.
authors.affiliation: Department of Neuroplasticity, Institute on Ageing and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan. suzukit@sch.md.shinshu-u.ac.jp
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Qing-Bao
authors.initials: QB
authors.lastname: Tian
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.forename: Nobuteru
authors.initials: N
authors.lastname: Usuda
authors.forename: Nobuteru
authors.initials: N
authors.lastname: Usada
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Fujii
authors.forename: Shogo
authors.initials: S
authors.lastname: Endo
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D017089:Ankyrin Repeat / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D003435:Crotalid Venoms / Q000235:genetics / Q000378:metabolism; D000076122:Disks Large Homolog 4 Protein; D004330:Drosophila; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism*; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D003435:Crotalid Venoms; D000076122:Disks Large Homolog 4 Protein; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D018091:Receptors, AMPA; C501449:Dlg4 protein, rat; C499875:TANC1 protein, rat; C033652:postsynaptic density proteins; C443104:rols protein, Drosophila