title: Effects of unsaturated fatty acids on calcium-activated potassium current in gastric myocytes of guinea pigs.
abstract: OBJECTIVE
  To investigate the effects of exogenous unsaturated fatty acids on calcium-activated potassium current (I(K(Ca))) in gastric antral circular myocytes of guinea pigs.
  
  
  METHODS
  Gastric myocytes were isolated by collagenase from the antral circular layer of guinea pig stomach. The whole-cell patch clamp technique was used to record I(K(Ca)) in the isolated single smooth muscle cells with or without different concentrations of arachidonic acid (AA), linoleic acid (LA), and oleic acid (OA).
  
  
  RESULTS
  AA at concentrations of 2,5 and 10 micromol/L markedly increased I(K(ca)) in a dose-dependent manner. LA at concentrations of 5, 10 and 20 micromol/L also enhanced I(K(Ca)) in a dose-dependent manner. The increasing potency of AA, LA, and oleic acid (OA) on I(K(Ca)) at the same concentration (10 micromol/L) was in the order of AA>LA>OA. AA (10 micromol/L)-induced increase of I(K(ca)) was not blocked by H-7 (10 micromol/L), an inhibitor of protein kinase C (PKC), or indomethacin (10 micromol/L), an inhibitor of the cyclooxygenase pathway, and 17-octadecynoic acid (10 micromol/L), an inhibitor of the cytochrome P450 pathway, but weakened by nordihydroguaiaretic acid (10 micromol/L), an inhibitor of the lipoxygenase pathway.
  
  
  CONCLUSIONS
  Unsaturated fatty acids markedly increase I(K(ca)), and the enhancing potencies are related to the number of double bonds in the fatty acid chain. The lipoxygenase pathway of unsaturated fatty acid metabolism is involved in the unsaturated fatty acid-induced increase of I(K(ca)) in gastric antral circular myocytes of guinea pigs.
authors.affiliation: Shanghai Jiaotong University School of Medicine, Shanghai 200030, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zheng
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Li
authors.forename: Zheng-Yuan
authors.initials: ZY
authors.lastname: Jin
authors.forename: Jia-Bin
authors.initials: JB
authors.lastname: Sun
authors.forename: Zai-Liu
authors.initials: ZL
authors.lastname: Li
authors.forename: Wen-Xie
authors.initials: WX
authors.lastname: Xu
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D019787:Linoleic Acid / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology*; D019301:Oleic Acid / Q000494:pharmacology; D024681:Potassium Channels, Calcium-Activated / Q000502:physiology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011706:Pyloric Antrum / Q000166:cytology* / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D005231:Fatty Acids, Unsaturated; D024681:Potassium Channels, Calcium-Activated; D016718:Arachidonic Acid; D019301:Oleic Acid; D019787:Linoleic Acid; D011493:Protein Kinase C