Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting.
Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.
Magni
Fulvio
F
Department of Experimental, Environmental Medicine and Medical Biotechnologies, University of Milano-Bicocca, Monza, Italy. fulvio.magni@unimib.it
Sarto
Cecilia
C
Valsecchi
Cristina
C
Casellato
Stefano
S
Bogetto
Stefano Ferrero
SF
Bosari
Silvano
S
Di Fonzo
Andrea
A
Perego
Roberto A
RA
Corizzato
Matteo
M
Doro
Giancarlo
G
Galbusera
Carmen
C
Rocco
Francesco
F
Mocarelli
Paolo
P
Galli Kienle
Marzia
M
D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007672:Kidney Cortex / Q000737:chemistry*; D010449:Peptide Mapping; D020033:Protein Isoforms / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D012016:Reference Values; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020033:Protein Isoforms; D020543:Proteome