title: The effect of IL-13 and IL-13R130Q, a naturally occurring IL-13 polymorphism, on the gene expression of human airway smooth muscle cells.
abstract: BACKGROUND
  Growing evidence shows that interleukin 13 (IL-13) may play an essential role in the development of airway inflammation and bronchial hyper-responsiveness (BHR), two defining features of asthma. Although the underlying mechanisms remain unknown, a number of reports have shown that IL-13 may exert its deleterious effects in asthma by directly acting on airway resident cells, including epithelial cells and airway smooth muscle cells. In this report, we hypothesize that IL-13 may participate in the pathogenesis of asthma by activating a set of "pro-asthmatic" genes in airway smooth muscle (ASM) cells.
  
  
  METHODS
  Microarray technology was used to study the modulation of gene expression of airway smooth muscle by IL-13 and IL-13R130Q. TaqMan Real Time PCR and flow cytometry was used to validate the gene array data.
  
  
  RESULTS
  IL-13 and the IL-13 polymorphism IL-13R130Q (Arg130Gln), recently associated with allergic asthma, seem to modulate the same set of genes, which encode many potentially interesting proteins including vascular cellular adhesion molecule (VCAM)-1, IL-13Ralpha2, Tenascin C and Histamine Receptor H1, that may be relevant for the pathogenesis of asthma.
  
  
  CONCLUSIONS
  The data supports the hypothesis that gene modulation by IL-13 in ASM may be essential for the events leading to the development of allergic asthma.
authors.affiliation: Centocor Inc,, 200 Great Valley Parkway, Malvern, PA 19355, USA. fsyed@cntus.jnj.com
authors.forename: Farhat
authors.initials: F
authors.lastname: Syed
authors.forename: Reynold A
authors.initials: RA
authors.lastname: Panettieri
authors.forename: Omar
authors.initials: O
authors.lastname: Tliba
authors.forename: Chris
authors.initials: C
authors.lastname: Huang
authors.forename: Katherine
authors.initials: K
authors.lastname: Li
authors.forename: Michelle
authors.initials: M
authors.lastname: Bracht
authors.forename: Bernard
authors.initials: B
authors.lastname: Amegadzie
authors.forename: Don
authors.initials: D
authors.lastname: Griswold
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yassine
authors.initials: Y
authors.lastname: Amrani
mesh_terms: D002478:Cells, Cultured; D004252:DNA Mutational Analysis; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics* / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D011110:Polymorphism, Genetic; D014132:Trachea / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D018793:Interleukin-13; D019010:Vascular Cell Adhesion Molecule-1