A simple method to study cellular migration.
We describe here a simple and fast method for the characterisation of cell motion. By projecting on a single plane different positions of the cell a ribbon is generated, whose characteristics can be related to the type of motion. The proposed method allows both to determine, very quickly, the motility of a population of cells and to investigate and characterise properties of a single cell's motion. The methodology presented here can be applied to a large range of cell movement and also adapted and extended to other problems involving biological motion.
Ariano
P
P
Universita' di Torino, Dipartimento di Biologia Animale e dell'Uomo and INFM, Turin, Italy.
Distasi
C
C
Gilardino
A
A
Zamburlin
P
P
Ferraro
M
M
D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D003196:Computer Graphics; D003198:Computer Simulation; D004305:Dose-Response Relationship, Drug; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008954:Models, Biological; D009414:Nerve Growth Factors / Q000494:pharmacology
D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D005346:Fibroblast Growth Factors