Functional link between ribosome formation and biogenesis of iron-sulfur proteins.
In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron-sulfur (Fe-S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe-S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe-S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in pre-rRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe-S proteins.
Yarunin
Alexander
A
Biochemie-Zentrum der Universität Heidelberg (BZH), Heidelberg, Germany.
Panse
Vikram Govind
VG
Petfalski
Elisabeth
E
Dez
Christophe
C
Tollervey
David
D
Hurt
Eduard C
EC
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001693:Biological Transport, Active; D004271:DNA, Fungal / Q000235:genetics; D039621:Eukaryotic Initiation Factor-3 / Q000235:genetics / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D004271:DNA, Fungal; D039621:Eukaryotic Initiation Factor-3; D007506:Iron-Sulfur Proteins; C102265:NBP35 protein, S cerevisiae; C492990:RLI1 protein, S cerevisiae; D012322:RNA Precursors; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; D019204:GTP-Binding Proteins