title: Determination of bulleyaconitine A in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry.
abstract: A sensitive and specific high-performance liquid chromatography (HPLC)-electrospray ionization tandem mass spectrometry (MS-MS) was developed for the determination of bulleyaconitine A (BLA) in human plasma. BLA and internal standard (I.S.) ketoconazole were extracted from the plasma by a liquid-liquid extraction. The supernatant was evaporated to complete dryness and reconstituted with acetonitrile containing 0.1% acetic acid before injecting into an ODS MS column. The gradient mobile phase was composed of a mixture of acetonitrile (containing 0.1% acetic acid, v/v) and 0.1% acetic acid aqueous solution eluted at 0.3 ml/min. BLA and I.S. were determined by multiple reaction monitoring using precursor-->product ion combinations at m/z 644.6-->584.3 and 531.2-->81.6, respectively. Linearity was established for the concentration range of 0.12-6 ng/ml. The recoveries of BLA ranged from 96.93 to 113.9% and the R.S.D. was within 20%. The method is rapid and applicable to the pharmacokinetic studies of BLA in human.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 200032, PR China.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Weng
authors.forename: Huinan
authors.initials: H
authors.lastname: Xu
authors.forename: Jianming
authors.initials: J
authors.lastname: Huang
authors.forename: Guoquan
authors.initials: G
authors.lastname: Wang
authors.forename: Teng
authors.initials: T
authors.lastname: Shen
authors.forename: Jianfang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C052509:bulleyaconitine A; D000157:Aconitine