title: [Dynamic expression of matrix metalloproteinase-2, membrane type-matrix metalloproteinase-2 in experimental hepatic fibrosis and its reversal in rat].
abstract: OBJECTIVE
  To investigate the expression dynamics and significance of matrix metalloproteinase-2 (MMP-2) membrane type-matrix metalloproteinase-2 (MT-MMP-2) in hepatic fibrosis and its reversal counterpart.
  
  
  METHODS
  An experimental CCl4 induced hepatic fibrosis rat model was established by intraperitoneal administration of carbon tetrachloride for 2, 4, 6, 8, 10 weeks, and normal rats were used as a control group. The immunohistochemical methods and in situ hybridization were used to detect MMP-2,MT-MMP-2 mRNA and related antigens in the liver.
  
  
  RESULTS
  MMP-2,MT-MMP-2 mRNA and related antigens were expressed in mesenchymal cells and parts of hepatocytes besides active pathological changes, especially in the fibrous septum and portal area. Expression of MMP-2,MT-MMP-2 mRNA and related antigens were increased in hepatic fibrosis and decreased gradually in its reversal counterpart.
  
  
  CONCLUSIONS
  This study suggested that mesenchymal cells are the main cellular origins of MMPs. The levels of MMP-2 and MT-MMP-2 antigens and gene expression were closely related to hepatic fibrosis. MMP-2 and MT-MMP-2 may play important roles in hepatic fibrosis and its reversal counterpart.
authors.affiliation: The No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Zhi-hai
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Shao-jie
authors.initials: SJ
authors.lastname: Xin
authors.forename: Jing-min
authors.initials: JM
authors.lastname: Zhao
authors.forename: Song-shan
authors.initials: SS
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Tie-yong
authors.initials: TY
authors.lastname: Yin
authors.forename: Guang-de
authors.initials: GD
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002252:Carbon Tetrachloride Poisoning; D015971:Gene Expression Regulation, Enzymologic; D022781:Hepatocytes / Q000201:enzymology; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000235:genetics; D053504:Matrix Metalloproteinases, Membrane-Associated; D059630:Mesenchymal Stem Cells / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D020782:Matrix Metalloproteinases; D053504:Matrix Metalloproteinases, Membrane-Associated; D020778:Matrix Metalloproteinase 2