title: [Molecular screening for moyamoya disease by use of expressed sequence tag on chromosome 3p].
abstract: Moyamoya disease is a well-known cerebrovascular disorder of unknown pathogenesis affecting terminal portion of internal carotid arteries and causing ischemic attacks. Its familial occurrence suggests genetic background. We hypothesized that paternally imprinted gene might be associated with this disorder. To identify the expressed sequence tags (ESTs) with monoallelic expressions on chromosome 3, we used mouse A9 hybrid cells having human chromosome 3. Two ESTs showed only maternal expression in mouse A9 hybrid cells, and four showed non-expression in the lymphocytes derived from moyamoya patients. Although these ESTs are clustered on the same 150 kb region, we finally failed to identify cDNA in this region.
authors.affiliation: Division of Medical Genetics, Kanagawa Children's Medical Center, Yokohama, Kanagawa. tyamamoto-jes@umin.ac.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Akasaka
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Ohtani
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Shiro
authors.initials: S
authors.lastname: Kashiwagi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ichiyama
authors.forename: Miki
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kato
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Maegaki
authors.forename: Akira
authors.initials: A
authors.lastname: Oka
authors.forename: Kousaku
authors.initials: K
authors.lastname: Ohno
mesh_terms: D000328:Adult; D002648:Child; D002893:Chromosomes, Human, Pair 3; D020224:Expressed Sequence Tags; D005260:Female; D006801:Humans; D008297:Male; D009072:Moyamoya Disease / Q000235:genetics*; D010375:Pedigree