TGF-beta1-enhanced TCP-coated sensate scaffolds can detect bone bonding.
Porous polybutylene terephthalate (PBT) scaffold systems were tested as orthopedic implants to determine whether these scaffolds could be used to detect strain transfer following bone growth into the scaffold. Three types of scaffold systems were tested: porous PBT scaffolds, porous PBT scaffolds with a thin beta-tricalcium phosphate coating (LC-PBT), and porous PBT scaffolds with the TCP coating vacuum packed into the scaffold pores (VI-PBT). In addition, the effect of applying TGF-beta1 to scaffolds as an enhancement was examined. The scaffolds were placed onto the femora of rats and left in vivo for 4 months. The amount of bone ingrowth and the strain transfer through various scaffolds was evaluated by using scanning electron microscopy, histology, histomorphometry, and cantilever bend testing. The VI-PBT scaffold showed the highest and most consistent degree of mechanical interaction between bone and scaffold, providing strain transfers of 68.5% (+/-20.6) and 79.2% (+/-8.7) of control scaffolds in tension and compression, respectively. The strain transfer through the VI-PBT scaffold decreased to 29.1% (+/-24.3) and 30.4% (+/-25.8) in tension and compression when used with TGF-beta1. TGF-beta1 enhancement increased the strain transfer through LC-PBT scaffolds in compression from 9.4% (+/-8.7) to 49.7% (+/-31.0). The significant changes in mechanical strain transfer through LC-PBT and VI-PBT scaffolds correlated with changes in bone ingrowth fraction, which was increased by 39.6% in LC-PBT scaffolds and was decreased 21.3% in VI-PBT scaffolds after TGF-beta1 enhancement. Overall, the results indicate that strain transfer through TCP-coated PBT scaffolds correlate with bone ingrowth after implantation, making these instrumented scaffolds useful for monitoring bone growth by monitoring strain transfer.
Szivek
J A
JA
Orthopedic Research Laboratory, Department of Orthopedic Surgery, Arizona Arthritis Center, College of Medicine, University of Arizona, P.O. Box 245194, Tucson, Arizona 85724, USA. szivek@u.arizona.edu
Margolis
D S
DS
Garrison
B K
BK
Nelson
E
E
Vaidyanathan
R K
RK
DeYoung
D W
DW
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001846:Bone Development; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology*; D001842:Bone and Bones / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry*; D005269:Femur / Q000473:pathology; D008422:Materials Testing; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry; D051381:Rats; D012984:Software; D013314:Stress, Mechanical; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C041733:poly(1,4-butylene terephthalate); C018392:tricalcium phosphate