Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae.
Three novel heterotrophic, Gram-negative, yellow-pigmented, aerobic, gliding, oxidase- and catalase-positive bacteria were isolated from algae collected in the Gulf of Peter the Great, Sea of Japan. 16S rRNA gene sequence analysis revealed that the strains studied represented members of the family Flavobacteriaceae and showed 93.5-93.8 % similarity with their closest relative, Psychroserpens burtonensis. The DNA G+C content of the strains was 34-37 mol%. The major respiratory quinone was MK-6. The predominant fatty acids were iso-C(15 : 0), anteiso-C(15 : 0), iso-C(15 : 1), iso-C(16 : 0)-3OH and iso-C(17 : 0)-3OH. On the basis of their phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the newly described bacteria have been assigned to the new genus Winogradskyella gen. nov., as Winogradskyella thalassocola sp. nov. (type strain, KMM 3907T=KCTC 12221T=LMG 22492T=DSM 15363T), Winogradskyella epiphytica sp. nov. (type strain, KMM 3906T=KCTC 12220T=LMG 22491T=CCUG 47091T) and Winogradskyella eximia sp. nov. (type strain, KMM 3944T (=KCTC 12219T=LMG 22474T).
Nedashkovskaya
Olga I
OI
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 let Vladivostoku 159, 690022, Vladivostok, Russia.
Kim
Seung Bum
SB
Korean Collection for Type Cultures, Biological Resource Center, Korea Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Han
Suk Kyun
SK
Korean Collection for Type Cultures, Biological Resource Center, Korea Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Snauwaert
Cindy
C
BCCM/LMG Bacteria Collection, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Vancanneyt
Marc
M
BCCM/LMG Bacteria Collection, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Swings
Jean
J
Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.|BCCM/LMG Bacteria Collection, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Kim
Kyung-Ok
KO
Korean Collection for Type Cultures, Biological Resource Center, Korea Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Lysenko
Anatoly M
AM
Institute of Microbiology of the Russian Academy of Sciences, Pr. 60 let October 7/2, Moscow, 117811, Russia.
Rohde
Manfred
M
Bereich Mikrobiologie, Abt. Mikrobielle Pathogenitat und Impfstoffforschung, GBF - Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Frolova
Galina M
GM
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 let Vladivostoku 159, 690022, Vladivostok, Russia.
Mikhailov
Valery V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 let Vladivostoku 159, 690022, Vladivostok, Russia.
Bae
Kyung Sook
KS
Korean Collection for Type Cultures, Biological Resource Center, Korea Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D056890:Eukaryota / Q000382:microbiology*; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S