Up-regulation of pathogenesis-related proteins in the apoplast of Malus domestica after application of a non-pathogenic bacterium.
The intercellular washing fluid (IWF) of Malus domestica cv. Holsteiner Cox before and after application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the leaves was investigated in a comparative manner. SDS-PAGE in combination with ESI Q-ToF mass spectrometry, and homology search in relevant data bases revealed the highly up-regulated expression of several pathogenesis-related plant proteins in the apoplast of the leaves treated with P. fluorescens. These proteins were beta3-1,3-glucanase, chitinase, thaumatin-like protein, ribonuclease-like protein, and a hevein-like protein. Moreover, a 9 kDa non-specific lipid transfer protein was significantly reduced after the application of P. fluorescens. The possible relevance of a pre-treatment of apple cultivars with the non-pathogenic bacterium P. fluorescens Bk3, as an alternative method to the treatment with fungicides, for increasing the resistance of susceptible apple cultivars against an infection with the fungus Venturia inaequalis is discussed.
Kürkcüoglu
Sophia
S
Institut für Botanik, Universität Hannover, D-30419 Hannover, Germany.
Piotrowski
Markus
M
Gau
Achim E
AE
D000595:Amino Acid Sequence; D018506:Gene Expression Regulation, Plant; D027845:Malus / Q000235:genetics* / Q000382:microbiology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011551:Pseudomonas fluorescens / Q000254:growth & development*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D010446:Peptide Fragments; D010940:Plant Proteins