title: Characterization of a novel pestivirus originating from a pronghorn antelope.
abstract: A unique pestivirus, isolated from a pronghorn antelope (Antilocopra americana), was characterized. Serum neutralization studies suggested that this virus was antigenically related to pestiviruses. Genomic characteristics, unique to pestiviruses, indicated that this virus belongs to the Pestivirus genus. These characteristics included the organization of the 5' untranslated region (5'-UTR), the presence and length of a viral Npro coding region, conservation of cysteine residues in Npro, conservation of predicted amino acid sequences flanking the cleavage sites between viral polypeptides Npro and C and between C and Erns and conservation of predicted hydrophobicity plots of Npro protein. While this data indicated the virus belongs to the Pestivirus genus, phylogenetic analysis in 5'-UTR, Npro and E2 regions suggested that it is the most divergent of the pestiviruses identified to date. This conclusion was also supported by the amino acid identity in coding regions. The corresponding values were much lower for the comparison of pronghorn pestivirus to other pestivirus genotypes than only between previous recognized genotypes. These results suggest the virus isolated from pronghorn antelope represents a new pestivirus genotype. It also represents the only pestivirus genotype first isolated from New World wildlife.
authors.affiliation: University of Veterinary Medicine, Department of Parasitology and Infectious Diseases, Komenskeho 73, 04181 Kosice, Slovakia. vilcek@uvm.sk
authors.forename: S
authors.initials: S
authors.lastname: Vilcek
authors.forename: J F
authors.initials: JF
authors.lastname: Ridpath
authors.forename: H
authors.initials: H
authors.lastname: Van Campen
authors.forename: J L
authors.initials: JL
authors.lastname: Cavender
authors.forename: J
authors.initials: J
authors.lastname: Warg
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000866:Antelopes / Q000821:virology*; D001483:Base Sequence; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010576:Pestivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D018182:Pestivirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D011499:Protein Processing, Post-Translational; D017385:Sequence Homology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D014764:Viral Proteins