title: The transcription factor profile of human mast cells in comparison with monocytes and granulocytes.
abstract: Expression profiles of mRNAs and proteins for various transcription factors were determined for human skin mast cells (sMCs), leukemic HMC-1 MCs, monocytes and granulocytes. By quantitative RT-PCR, sMCs expressed lower levels of c-fos, PU.1, C/EBPalpha, and C/EBPepsilon than monocytes and granulocytes, but higher levels of MITF, SCL, GATA-1 and GATA-2. At the protein level, MITF, SCL, GATA-2, Elf-1 and c-fos were clearly detectable in sMCs. With the exception of c-fos, these proteins were absent or expressed only slightly in monocytes and granulocytes. The expression of NF-E2p45, GATA-1, PU.1, Ets-1, C/EBPalpha and C/EBPepsilon was below the detection limit in sMCs, but detectable in other myelocytes. The high expression of SCL and GATA-2 in sMCs is reminiscent of stem cells. The absence of C/EBPvarepsilon in sMCs, but strong expression in HMC-1, suggests it may impair MC maturation. In summary, mature human MCs can be characterized as C/EBPalpha(low), C/EBPvarepsilon-, PU.1(low), GATA-1(low), GATA-2+, SCL+, MITF(high).
authors.affiliation: Department of Dermatology and Allergy, University Hospital Charité Campus Mitte, Schumannstr. 20/21, 10117, Berlin, Germany. magda.babina@charite.de
authors.forename: M
authors.initials: M
authors.lastname: Babina
authors.forename: Y
authors.initials: Y
authors.lastname: Schülke
authors.forename: L
authors.initials: L
authors.lastname: Kirchhof
authors.forename: S
authors.initials: S
authors.lastname: Guhl
authors.forename: R
authors.initials: R
authors.lastname: Franke
authors.forename: S
authors.initials: S
authors.lastname: Böhm
authors.forename: T
authors.initials: T
authors.lastname: Zuberbier
authors.forename: B M
authors.initials: BM
authors.lastname: Henz
authors.forename: A F
authors.initials: AF
authors.lastname: Gombart
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D050989:GATA2 Transcription Factor; D020869:Gene Expression Profiling; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D051705:Sp3 Transcription Factor; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; C494712:Gata2 protein, mouse; C494713:Gata2 protein, rat; D014157:Transcription Factors; D051705:Sp3 Transcription Factor; D011494:Protein Kinases; C065999:Sp1 kinase