title: Resin monomer 2-hydroxyethyl methacrylate (HEMA) is a potent inducer of apoptotic cell death in human and mouse cells.
abstract: Mechanisms by which the resin monomer 2-hydroxyethyl methacrylate (HEMA) induces hypersensitivity reactions in humans are not well-established, nor have the direct effects of HEMA on cell death been fully characterized. The objective of this study was to establish whether HEMA is capable of inducing apoptotic cell death, and whether differences exist in the levels of apoptotic death induced by HEMA in cells obtained from healthy individuals and from patients with established HEMA hypersensitivity. HEMA induced apoptotic death in Peripheral Blood Mononuclear Cells (PBMCs) obtained from both healthy and HEMA-sensitized patients and in the murine RAW cells in a dose-dependent manner. However, induction of cell death by HEMA was lower in PBMCs obtained from patients in comparison with healthy individuals. Studies reported in this paper demonstrate that HEMA induces apoptotic death, and that decreased susceptibility of lymphocytes to HEMA-mediated death might be an important mechanism for the generation and persistence of hypersensitivity reactions in patients.
authors.affiliation: Division of Oral Biology and Oral Medicine, UCLA School of Dentistry, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Los Angeles, CA 90095, USA.
authors.forename: A
authors.initials: A
authors.lastname: Paranjpe
authors.forename: L C F
authors.initials: LC
authors.lastname: Bordador
authors.forename: M-Y
authors.initials: MY
authors.lastname: Wang
authors.forename: W R
authors.initials: WR
authors.lastname: Hume
authors.forename: A
authors.initials: A
authors.lastname: Jewett
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D002460:Cell Line; D003188:Composite Resins / Q000009:adverse effects*; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects; D016555:Matched-Pair Analysis; D008689:Methacrylates / Q000009:adverse effects*; D051379:Mice; D009000:Monocytes / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000009:adverse effects; D011109:Polymethacrylic Acids / Q000009:adverse effects
chemical_list: D003188:Composite Resins; D008689:Methacrylates; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate