Fluid mixing in growing microscale vesicles conjugated by surfactant nanotubes.
This work addresses novel means for controlled mixing and reaction initiation in biomimetic confined compartments having volume elements in the range of 10(-12) to 10(-15) L. The method is based on mixing fluids using a two-site injection scheme into growing surfactant vesicles. A solid-state injection needle is inserted into a micrometer-sized vesicle (radius 5-25 microm), and by pulling on the needle, we create a nanoscale surfactant channel connecting injection needle and the vesicle. Injection of a solvent A from the needle into the nanotube results in the formation of a growing daughter vesicle at the tip of the needle in which mixing takes place. The growth of the daughter vesicle requires a flow of surfactants in the nanotube that generates a flow of solvent B inside the nanotube which is counterdirectional to the pressure-injected solvent. The volume ratio psi between solvent A and B inside the mixing vesicle was analyzed and found to depend only on geometrical quantities. The majority of fluid injected to the growing daughter vesicle comes from the pressure-based injection, and for a micrometer-sized vesicle it dominates. For the formation of one daughter vesicle (conjugated with a 100-nm radius tube) expanded from 1 to 200 microm in radius, the mixing ratios cover almost 3 orders of magnitude. We show that the system can be expanded to linear strings of nanotube-conjugated vesicles that display exponential dilution. Mixing ratios spanning 6 orders of magnitude were obtained in strings of three nanotube-conjugated micrometer-sized daughter vesicles.
Davidson
Max
M
Department of Physical Chemistry and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.
Dommersnes
Paul
P
Markström
Martin
M
Joanny
Jean-Francois
JF
Karlsson
Mattias
M
Orwar
Owe
O
D005456:Fluorescent Dyes / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008956:Models, Chemical; D043942:Nanotubes / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D010835:Phytohemagglutinins
D005456:Fluorescent Dyes; D008081:Liposomes; D037121:Plant Lectins; D030262:Soybean Proteins; D013501:Surface-Active Agents; C028824:soybean lectin; D010835:Phytohemagglutinins