title: Effect of long-term cold exposure on activities of cytochrome P450-containing monooxygenases and glutathione S-transferase in rat liver microsomes.
abstract: Long-term cold exposure (5 degrees C) was followed by induction of rat liver monooxygenases. We revealed an increase in activity of NADPH-cytochrome C reductase, total content of cytochrome P450 (CYP), and activities of its molecular forms CYP1A1, 1A2, 2B1/B2, 2E1, and 3A1/A2 in microsomes. These indexes reached maximum by the 10th day, but decreased with lengthening of cold exposure. Glutathione S-transferase activity decreased under these conditions. Changes in enzyme activity could be related to the increase in blood corticosterone concentration.
authors.affiliation: Institute of Molecular Biology and Biophysics, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Gromova
authors.forename: A Yu
authors.initials: AY
authors.lastname: Grishanova
authors.forename: M L
authors.initials: ML
authors.lastname: Perepechaeva
authors.forename: N G
authors.initials: NG
authors.lastname: Kolosova
authors.forename: A R
authors.initials: AR
authors.lastname: Kolpakov
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003345:Corticosterone / Q000097:blood; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase; D005982:Glutathione Transferase; D003345:Corticosterone