Sample preparation including sol-gel immunoaffinity chromatography for determination of bisphenol A in canned beverages, fruits and vegetables.
The paper describes the development of a very simple method to prepare samples of canned food (beverages, fruits and vegetables) for the determination of bisphenol A by isocratic HPLC with fluorescence detection. The new sample preparation method makes use of the selectivity of bisphenol A antibodies immobilized in a silica matrix by an inexpensive and simple sol-gel technique. In spite of applying highly complex food matrices, immunoaffinity columns could be used for clean-up of at least 15 real samples. Limits of detection (S/N=3) ranged from 0.1 ng/ml for beverages to 4.3 ng/g for vegetables.
Braunrath
R
R
Institute of Analytical Chemistry, University of Vienna, Austria.
Cichna
M
M
D001559:Benzhydryl Compounds; D001628:Beverages / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D005782:Gels; D010636:Phenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014675:Vegetables / Q000737:chemistry*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D005782:Gels; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds