Experimental study of the killing effects of oxymatrine on human colon cancer cell line SW1116.
OBJECTIVE
To study the killing effects of oxymatrine (OM) on a human colon cancer cell line, SW1116, and evaluate its antineoplastic mechanism.


METHODS
Methyl thiazolyl tetrazolium (MTT) analysis, flow cytometry, polymerase chain reaction (PCR)-enzyme linked immunosorbent assay (ELISA) and RT-PCR methods were used respectively to determine the killing effects of OM and its influence on cell cycle distribution, telomerase activity and the expressions of hTERT, c-myc, p53 and mad1 in SW1116 cells.


RESULTS
Oxymatrine exhibited dose-dependent killing effects on SW1116 cells and induced G1/G0-phase arrest. It suppressed the telomerase activity of the cells in a dose- and time-dependent manner. After OM administration, the expression of hTERT in the SW1116 cells decreased, those of p53 and mad1 increased, and the expression of c-myc was unchanged.


CONCLUSIONS
Oxymatrine has dose-dependent killing effects on SW1116 cells and its antineoplastic activity might be attributed to inhibition of telomerase activity by means of its effects on hTERT and the upstream regulating genes.
Zou
Jian
J
Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai, China.
Ran
Zhi Hua
ZH
Xu
Qi
Q
Xiao
Shu Dong
SD
D000470:Alkaloids / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D016923:Cell Death; D003110:Colonic Neoplasms / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D011807:Quinolizines; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000093842:Matrines
D000470:Alkaloids; D000998:Antiviral Agents; D011807:Quinolizines; D019098:Telomerase; C037573:oxymatrine; D000093842:Matrines