Swietenia mahagony extract shows agonistic activity to PPAR(gamma) and gives ameliorative effects on diabetic db/db mice.
OBJECTIVE
To search the peroxisome proliferator-activated receptor g (PPAR(gamma)) agonists from Swietenia mahagony extract (SmE) and observe the possible ameliorative effects of SmE on diabetic db/db mice.


METHODS
The PPAR(gamma) agonistic activity of SmE was screened by yeast-two hybrid system. The blood glucose levels of diabetic db/db mice were measured using a blood glucose level monitor and the data were statistically analyzed by NDST8.8W software.


RESULTS
By using the clinical drug rosiglitazone as a positive control, it was found that the PPARg agonistic activity of SmE at a concentration of 50 microg/L was approximately half that of 35.7 microg/L (0.1 micromol/L) of rosiglitazone. At the dose of 1000 mg/kg, SmE remarkably decreased the blood glucose concentration of db/db mice from (15.26+/-2.98) to (7.58+/-2.20) mmol/L, and reduced the blood glucose levels by 55.49% compared with the control group (P<0.01).


CONCLUSIONS
SmE shows agonistic activity to PPARg and can ameliorate the blood glucose levels of diabetic db/db mice. SmE may be thus used as a potential agent for diabetes therapy.
Li
Dan-Dan
DD
Drug Discovery and Design Center, State Key Lab of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
Chen
Jun-Hua
JH
Chen
Qing
Q
Li
Guo-Wei
GW
Chen
Jing
J
Yue
Jian-Min
JM
Chen
Min-Li
ML
Wang
Xiao-Ping
XP
Shen
Jian-Hua
JH
Shen
Xu
X
Jiang
Hua-Liang
HL
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D029582:Meliaceae / Q000737:chemistry; D051379:Mice; D047495:PPAR gamma / Q000819:agonists*; D010946:Plants, Medicinal / Q000737:chemistry; D012639:Seeds
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D047495:PPAR gamma