Preparation and properties of gelatin-immobilized beta-glucosidase from Pyrococcus furiosus.
Hyperthermostable beta-glucosidase from Pyrococcus furiosus was enclosed in gelatin gel by cross-linking with transglutaminase. Gelatin-immobilized beta-glucosidase was considerably more thermostable than the native enzyme. Lyophilized immobilisate was stored at 90 degrees C for 1 month without loss of activity. The immobilized beta-glucosidase catalyzed transglucosylation of 5-phenylpentanol with 10.0 equivalent of cellobiose at pH 5.0 and 70 degrees C for 12 h to afford 5-phenylpentyl beta-D-glucopyranoside in 41% yield. The immobilized enzyme was more effective than the native one in transglucosylation. The gelatin-immobilized Pfu-beta-glucosidase recovered from the first run of the reaction was reusable on successive runs.
Nagatomo
Hidetaka
H
Faculty of Engineering, University of Miyazaki, Miyazaki, Japan.
Matsushita
Yoh-ichi
Y
Sugamoto
Kazuhiro
K
Matsui
Takanao
T
D002475:Cellobiose / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry*; D005960:Glucosides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D000439:Pentanols / Q000378:metabolism; D020027:Pyrococcus furiosus / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005960:Glucosides; D000439:Pentanols; C017930:5-phenylpentan-1-ol; C033857:phenylglucoside; D002475:Cellobiose; C025193:4-nitrophenyl beta-D-glucoside; D005780:Gelatin; D001617:beta-Glucosidase