title: Induction of inducible nitric oxide (NO) synthase mRNA and NO production in macrophages infected with influenza A/PR/8 virus and stimulated with its ether-split product.
abstract: We investigated the inductive activity of infective influenza A/PR/8/34 (PR8) virus and its ether-split product (ESP) on the expression of inducible nitric oxide (NO) synthase (iNOS) and NO production in RAW264.7 (RAW) cells, a murine macrophage (M psi) cell line, and thioglycolate-elicited peritoneal M psi (TPM). In both cells, PR8 virus infection induced iNOS mRNA between 4 hr and 24 hr, attaining a peak value at 12 hr. In correlation with induction of iNOS mRNA, NO amounts increased significantly from 12 to 24 hr. Moreover, this study demonstrated that ESP with the same hemagglutination titer as PR8 virus could induce iNOS mRNA and NO production, although the inductive activity of ESP was weaker than that of PR8 virus. Considering the dual role (beneficial and detrimental roles) of NO on certain inflammatory disorders and virus infections, the inductive activity of influenza virus on the iNOS-mediated NO production independent of its infectivity might contribute to a modification of influenza virus infection.
authors.affiliation: Department of Japanese Oriental Medicine, Toyama Medical and Pharmaceutical University, Toyama, Toyama, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Imanishi
authors.forename: Tsugunobu
authors.initials: T
authors.lastname: Andoh
authors.forename: Shinya
authors.initials: S
authors.lastname: Sakai
authors.forename: Miyuki
authors.initials: M
authors.lastname: Satoh
authors.forename: Yuko
authors.initials: Y
authors.lastname: Katada
authors.forename: Kyouka
authors.initials: K
authors.lastname: Ueda
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Terasawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ochiai
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D008264:Macrophages / Q000201:enzymology / Q000378:metabolism* / Q000821:virology*; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D009975:Orthomyxoviridae / Q000276:immunology* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D014764:Viral Proteins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse