Imaging protein behavior inside the living cell.
The genetically encoded fluorescent proteins (FPs) have transformed studies in cell biology by allowing the behavior of proteins to be tracked within the natural environment of the living cell. Progressively more complex imaging methods are being used to measure the mobility, co-localization and interactions of proteins labeled with the FPs. This review provides an overview of recent developments in live-cell imaging techniques to analyze the subcellular distribution and interactions of proteins in living cells.
Day
Richard N
RN
Departments of Medicine and Cell Biology, University of Virginia Health System, Charlottesville, VA 22908-0578, USA. rnd2v@virginia.edu
D042541:Intracellular Space / Q000737:chemistry; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000032:analysis* / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D008164:Luminescent Proteins; D011506:Proteins