A proteomic strategy for the identification of caspase-associating proteins.
We report the efficient in vivo labelling of caspases expressed inside apoptotic HeLa cells using fluoromethyl ketone (fmk)-containing probes; preliminary results indicated that these probes may be used to identify caspase-associating proteins.
Tan
Eunice L P
EL
Department of Biological Scienes, National University of Singapore, Singapore 117543, Republic of Singapore.
Panicker
Resmi C
RC
Chen
Grace Y J
GY
Yao
Shao Q
SQ
D020169:Caspases / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics
D015335:Molecular Probes; D011506:Proteins; D020169:Caspases