title: Colonic short-chain fatty acids inhibit encystation of Entamoeba invadens.
abstract: Entamoeba parasites multiply as trophozoites in the layer of mucus that overlies the colonic epithelium. In response to stimuli that are not understood, trophozoites stop multiplying and differentiate into cysts that are released to infect another host. In the colon, Entamoeba trophozoites are exposed to the large variety of biochemicals that are carried into or are produced within this organ. The normal bacterial population of the colon releases large amounts of short-chain fatty acids (SCFAs). These compounds have effects on the growth, differentiation and repair of the colonic epithelium that correlate with de-creased activity of a Class I/II histone deacetylase (HDAC). We found that the formation of cysts, but not the growth of trophozoite-stage Entamoeba invadens parasites, was inhibited by physiologic concentrations of SCFAs. Variable levels of cyst formation did occur if SCFA concentrations were lowered. Specific inhibitors of Class I/II-type HDACs also prevented encystation, and trophozoites exposed to these compounds had increased levels of acetylation of histone H4 and other nuclear proteins. These results suggest that production of the infectious cyst stage of Entamoeba parasites is regulated in part by the levels of SCFAs made by the bacterial population of the colon.
authors.affiliation: Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Byers
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Faigle
authors.forename: Daniel
authors.initials: D
authors.lastname: Eichinger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D003106:Colon / Q000382:microbiology / Q000469:parasitology; D004747:Entamoeba / Q000254:growth & development*; D005232:Fatty Acids, Volatile / Q000378:metabolism / Q000502:physiology*; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D008969:Molecular Sequence Data
chemical_list: D005232:Fatty Acids, Volatile; D006657:Histones; D006655:Histone Deacetylases