Complete nucleotide sequence and genome organization of a dsRNA partitivirus infecting Pleurotus ostreatus.
The nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus (P. ostreatus virus 1; PoV1) were determined and compared to the sequences of the other mycoviruses belonging to partitiviruses and totivirues. PoV1 dsRNA-1 and dsRNA-2 had genomes of 2296 and 2223 nucleotides, respectively. The purified virus preparations contained isometric particles of 28-30 nm in diameter, and also the same two dsRNAs were isolated from purified virus preparations. The sequences of PoV1 dsRNA-1 and dsRNA-2 had GC contents of 48.4 and 51.5%, respectively. dsRNA-1 had 78 and 97 nucleotides of 5'- and 3'-untranslated region (UTR) while dsRNA-2 had 114 and 198 nucleotides of 5'- and 3'-UTR, respectively. Computer analysis of putative open reading frame (ORF) shows that dsRNA-1 and dsRNA-2 contain a single ORF encoding proteins of 82.2 and 71.1 kDa that show high sequence identity with RNA-dependent RNA polymerase and capsid protein of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis they were found to form a distinct virus clade with partitiviruses, and were more distantly related to totiviruses.
Lim
Won-Seok
WS
School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, South Korea.
Jeong
Ji Hye
JH
Jeong
Rae-Dong
RD
Yoo
Young Bok
YB
Yie
Se Won
SW
Kim
Kook-Hyung
KH
D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D017124:Conserved Sequence; D016679:Genome, Viral; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D020076:Pleurotus / Q000821:virology*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017385:Sequence Homology
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase