[Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion].
OBJECTIVE
To study effect of triptolide (TL) on neuronal apoptosis in cerebral tissue of rat after ischemia-reperfusion.


METHODS
Triptolide at dose 0.2 or 0.4 mg x kg(-1) was intraperitoneally injected once a day for 4 d. The focal cerebral ischemia-reperfusion model was established with thread embolism in middle artery before triptolide injection on the fourth day. Neurological deficit score of rats was evaluated; and immunohistochemical techniques were used to count positive cells of express of MPO and TUNEL in cerebraltissue.


RESULTS
Compared with the control group, the deficit of neural function was significantly improved, and the number of infiltrate of neutrophil and neuronal apoptosis in cerebral tissue was remarkably reduced in two TL-treated groups.


CONCLUSIONS
The results suggested that TL can inhibit infiltration of neutrophil and decrease the degree of neuronal apoptosis in cerebral tissue.
Wei
Deng-ming
DM
Medical School of Ningbo University, Ningbo 315211, China. weidengmin99@sohu.com
Huang
Guang-zhao
GZ
Zhang
Yi-gu
YG
Rao
Guang-xun
GX
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002545:Brain Ischemia / Q000473:pathology; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004852:Epoxy Compounds; D005260:Female; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020556:Neutrophil Infiltration / Q000187:drug effects; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*; D029757:Tripterygium / Q000737:chemistry
D004224:Diterpenes; D004852:Epoxy Compounds; D018696:Neuroprotective Agents; D010616:Phenanthrenes; C001899:triptolide