Disturbances in epithelial ionic secretion in different experimental models of colitis.
This paper studies the disturbances in ionic secretion in the colon of rats with different models of acute and chronic colitis measured as changes in short-circuit current. The aim was to verify whether the reported inhibition of basal and stimulated secretion in the trinitrobenzene sulfonic acid and mytomicin C models are applicable to experimental colitis as such. All models showed remarkable similarity in ion transport as determined in Ussing chambers, with downregulated basal as well as carbachol evoked secretion. The EC(50) of carbachol was unchanged in all cases. Iodoacetamide and oxazolone colitis models were notable exceptions in that the dose response curves for carbachol were unaltered compared to controls. The reason is unclear but seems to be unrelated to either interferon gamma or interleukin 4 levels or to the severity of the inflammatory response.
Pérez-Navarro
Rubén
R
Department of Pharmacology, School of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071 Granada, Spain. fsanchez@ugr.es
Ballester
Isabel
I
Zarzuelo
Antonio
A
Sánchez de Medina
Fermín
F
D019342:Acetic Acid; D000208:Acute Disease; D000477:Alkylating Agents; D000818:Animals; D030801:Animals, Genetically Modified; D002700:Chloramines; D002908:Chronic Disease; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D015796:HLA-B27 Antigen / Q000235:genetics; D007202:Indicators and Reagents; D007460:Iodoacetamide; D007477:Ions / Q000378:metabolism; D010081:Oxazolone; D018408:Patch-Clamp Techniques; D051381:Rats; D014302:Trinitrobenzenesulfonic Acid
D000477:Alkylating Agents; D002700:Chloramines; D015796:HLA-B27 Antigen; D007202:Indicators and Reagents; D007477:Ions; D010081:Oxazolone; D014302:Trinitrobenzenesulfonic Acid; D016264:Dextran Sulfate; C030816:chloramine; D019342:Acetic Acid; D007460:Iodoacetamide