title: [Cloning and sequencing of the upstream of mouse dentin sialophosphoprotein promoter].
abstract: OBJECTIVE
  To clone and sequence the upstream of mouse dentin sialophosphoprotein promoter.
  
  
  METHODS
  Genomic DNA was obtained from Balb/c mouse blood. The upstream of mouse dentin sialophosphoprotein promoter segments was obtained by PCR. Then the segments were inserted into T-vector. The plasmids were identified by digestion with the restriction enzyme analysis. The positive clone was sequenced and compared with Genebank.
  
  
  RESULTS
  The upstream of mouse dentin sialophosphoprotein promoter was divided into three sequences and three different target segments with 997 bp, 1004 bp and 674 bp in length were obtained. After identified, sequenced and compared with Genebank, the sequences of the segments were consistent with those displayed on Genebank by 99%.
  
  
  CONCLUSIONS
  The clone of the upstream of mouse dentin sialophosphoprotein promoter was successful. This work will help to study the regulation of DSPP expression.
authors.affiliation: Dept. of Conservative Dentistry, Faculty of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.forename: Qinq
authors.initials: Q
authors.lastname: Yu
authors.forename: Ming-zhen
authors.initials: MZ
authors.lastname: Xiao
authors.forename: Shou-liang
authors.initials: SL
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Qing-lin
authors.initials: QL
authors.lastname: Zhu
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010750:Phosphoproteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012795:Sialoglycoproteins / Q000235:genetics*; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein