Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b.
A novel gene for quinolone resistance was cloned from a transferable plasmid carried by a clinical isolate of Shigella flexneri 2b that was resistant to fluoroquinolones. The plasmid conferred low-level resistance to quinolones on Escherichia coli HB101. The protein encoded by the gene showed 59% amino acid identity with Qnr.
Hata
Mami
M
Department of Microbiology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsujimachi, Kita-ku, Nagoya, Aichi 462-8576, Japan. mami_hata@pref.aichi.lg.jp
Suzuki
Masahiro
M
Matsumoto
Masakado
M
Takahashi
Masao
M
Sato
Katsuhiko
K
Ibe
Shiro
S
Sakae
Kenji
K
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D015363:Quinolones / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012763:Shigella flexneri / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D015363:Quinolones