title: Toxigenic status of Clostridium difficile in a large Spanish teaching hospital.
abstract: The aim of this study was to evaluate the toxigenic status of circulating strains of Clostridium difficile in a large teaching hospital. Overall 220 isolates were studied of which 199 (90.5 %) produced both large clostridial toxins detected by conventional methods. Ten more strains (4.5 %) had toxin A and B genes detectable by PCR. Eleven (5.0 %) variant strains (A- B+) were detected among the isolates studied and 10 strains (4.5 %) had the binary toxin genes (cdtA and cdtB).
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ''Gregorio Marañón'', C/Doctor Esquerdo, 46, 28007 Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Alonso
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ''Gregorio Marañón'', C/Doctor Esquerdo, 46, 28007 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martín
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ''Gregorio Marañón'', C/Doctor Esquerdo, 46, 28007 Madrid, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Peláez
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ''Gregorio Marañón'', C/Doctor Esquerdo, 46, 28007 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Marín
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ''Gregorio Marañón'', C/Doctor Esquerdo, 46, 28007 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Creixéms
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ''Gregorio Marañón'', C/Doctor Esquerdo, 46, 28007 Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Bouza
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D016360:Clostridioides difficile / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis / Q000382:microbiology; D004768:Enterotoxins / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D006784:Hospitals, Teaching; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile