Prevention against oxidative stress of eukaryotic cell membranes by selenium compounds of the rat.
By a combination of trace techniques and various biochemical methods, information about the characteristics of a 15-kDa selenoprotein was obtained. After labeling of rats in vivo with [(75)Se]selenite, subcellular fractionation of the homogenates of the prostate, lung, brain, thyroid gland, and large intestine, and gel electrophoretic separation of the proteins and subcellular fractionation, 15-kDa (75)Se was found in the cytosols of the tissues prostate > brain > lung > thyroid gland > large intestine after autoradiography. After coelectrophoresis of the separated 15-kDa labeled band obtained from each cytosolic fraction, the 15-kDa (75)Se band migrated in the same way as the combined bands isolated from the five tissue cytosols. After proteolytic cleavage in the gel of the 15-kDa labeled band obtained from the cytosol of each tissue and re-electrophoresis, the same labeled peptide pattern was found in each gel slice after autoradiography. By means of reversed-phase HPLC, we characterized a selenocysteine-containing protein that has enzymatic activity like that of glutathione peroxidase.
Kyriakopoulos
A
A
Department of Molecular Trace Element Research in the Life Sciences, Hahn-Meitner-Institut, Glienicker Str. 100, D-14109 Berlin, Germany. kyriakopoulos@hmi.de
Bukalis
K
K
Roethlein
D
D
Hoppe
B
B
Graebert
A
A
Behne
D
D
D000818:Animals; D001345:Autoradiography; D002462:Cell Membrane / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D018036:Selenium Compounds / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018036:Selenium Compounds