High dose of Garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis.
We investigated the ability of Garcinia cambogia extract containing (-)-hydroxycitric acid (HCA) to suppress body fat accumulation in developing male Zucker obese (fa/fa) rats. We also examined histopathologically the safety of its high doses. Diets containing different levels of HCA (0, 10, 51, 102 and 154 mmol/kg diet) were fed to 6-week-old rats for 92 or 93 days. Each diet group was pair-fed to the 154 mmol HCA/kg diet group. Epididymal fat accumulation and histopathological changes in tissues were observed. The highest dose of HCA-containing Garcinia cambogia (154 mmol HCA/kg diet) showed significant suppression of epididymal fat accumulation in developing male Zucker obese rats, compared with the other groups. However, the diets containing 102 mmol HCA/kg diet and higher (778 and 1244 mg HCA/kg BW/d, respectively) caused potent testicular atrophy and toxicity, whereas diets containing 51 mmol HCA/kg diet (389 mg HCA/kg BW/d) or less did not. Accordingly, 51 mmol HCA/kg diet (389 mg HCA/kg BW/d) was deemed to be the no observed adverse effect level (NOAEL).
Saito
M
M
Division of Food Science, Incorporated Administrative Agency, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan. msaito@nih.go.jp
Ueno
M
M
Ogino
S
S
Kubo
K
K
Nagata
J
J
Takeuchi
M
M
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000633:toxicity; D002951:Citrates / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D029761:Garcinia cambogia / Q000737:chemistry*; D020738:Leptin / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D011924:Rats, Zucker; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000097:blood; D015430:Weight Gain / Q000187:drug effects*
D019440:Anti-Obesity Agents; D002951:Citrates; D020738:Leptin; D010936:Plant Extracts; D013739:Testosterone; C007999:hydroxycitric acid; D001275:ATP Citrate (pro-S)-Lyase