Estrogenic activity of a polyphenolic extract of the leaves of Epimedium brevicornum.
The estrogenic activity of a polyphenolic extract of the leaves of Epimedium brevicornum and five fractions obtained by solid-phase extraction were investigated using estrogen-responsive bioassays, a yeast cell assay and the Ishikawa Var-I assay. The extract was found to exhibit significant estrogenic activity in both assays. Furthermore, bioassay-guided fractionation led to localisation of the estrogenicity in the relatively non-polar fractions of the polyphenolic extract.
De Naeyer
An
A
Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Pharmacognosy and Phytochemistry, Harelbekestraat 72, B-9000 Ghent, Belgium.
Pocock
Victoria
V
Milligan
Stuart
S
De Keukeleire
Denis
D
D004305:Dose-Response Relationship, Drug; D034181:Epimedium; D006801:Humans; D048789:Phytoestrogens / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D011960:Receptors, Estrogen / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics
D048789:Phytoestrogens; D010936:Plant Extracts; D011960:Receptors, Estrogen