title: IgE, IgG1, and IgG4 antibody responses to Blomia tropicalis in atopic patients.
abstract: BACKGROUND
  Allergens from house dust mites (HDMs), Dermatophagoides pteronyssinus and Blomia tropicalis are clinically relevant in atopic respiratory diseases in tropical countries.
  
  
  OBJECTIVE
  To evaluate immunoglobulin (Ig)E, IgG1, and IgG4 antibody responses to B. tropicalis in Brazilian atopic patients.
  
  
  METHODS
  About 110 patients with allergic rhinitis with/without asthma and 33 control subjects underwent skin prick testing (SPT) with HDM extracts, and their sera were tested for IgE and IgG subclass antibodies to D. pteronyssinus and B. tropicalis by enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
  
  
  RESULTS
  Most patients (56%) had positive SPT to B. tropicalis extract (B. tropicalis+ group), although 51% were reactive to both B. tropicalis and D. pteronyssinus and 6% were sensitized to B. tropicalis only. IgE-ELISA detected 43%B. tropicalis positivity with high-specific IgE levels in B. tropicalis+ patients. Specific IgG4 levels were higher in B. tropicalis+ than B. tropicalis- groups and correlated with specific IgE levels. The IgG1 levels to B. tropicalis were higher in patients than controls. The major allergenic B. tropicalis components recognized by B. tropicalis+ patient sera were the 54, 66, and 68 kDa proteins. The IgG4-binding protein profiles closely resembled that of IgE. The IgG1 antibodies recognizing multiple B. tropicalis protein species were detected in sera of all three patient groups.
  
  
  CONCLUSIONS
  A large percentage of our allergic patients are B. tropicalis+. They are more frequently sensitized to high-molecular weight (HMW) B. tropicalis components than the major low-molecular weight (11-15 kDa) allergens detected in other studies. The results suggest that HMW B. tropicalis antigenic components are potential candidates for evaluating allergen exposure and sensitization, and for immunotherapy treatment.
authors.affiliation: Division of Immunology, Institute of Biomedical Sciences, Federal University of Uberlândia, MG, Brazil.
authors.forename: E A L
authors.initials: EA
authors.lastname: Pereira
authors.forename: D A O
authors.initials: DA
authors.lastname: Silva
authors.forename: J P
authors.initials: JP
authors.lastname: Cunha-Júnior
authors.forename: K C
authors.initials: KC
authors.lastname: Almeida
authors.forename: R
authors.initials: R
authors.lastname: Alves
authors.forename: S J
authors.initials: SJ
authors.lastname: Sung
authors.forename: E A
authors.initials: EA
authors.lastname: Taketomi
mesh_terms: D000328:Adult; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D001249:Asthma / Q000209:etiology; D016022:Case-Control Studies; D004391:Dust; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D008925:Mites / Q000276:immunology*; D008970:Molecular Weight; D012221:Rhinitis, Allergic, Perennial / Q000150:complications / Q000276:immunology
chemical_list: D000941:Antigens; D004391:Dust; D007074:Immunoglobulin G; D007073:Immunoglobulin E