Frequent infection of Hylobates pileatus (pileated gibbon) with species-associated variants of hepatitis B virus in Cambodia.
As well as being distributed widely in human populations, hepatitis B virus (HBV) infections occur frequently in chimpanzee, gibbon and other ape populations in sub-Saharan Africa and South-East Asia. To investigate the frequency and genetic relationships of HBV infecting gibbons in Cambodia, pileated gibbons (Hylobates pileatus) that were originally wild-caught were screened for surface antigen. Twelve of 26 (46 %) were positive, of which 11 were positive for HBV DNA. Phylogenetic analysis of complete genome sequences revealed two distinct genetic groups in the gibbon/orangutan clade. Three were similar to previously described variants infecting H. pileatus in Thailand and eight formed a distinct clade, potentially representing distinct strains of HBV circulating in geographically separated populations in South-East Asia. Because of the ability of HBV to cross species barriers, large reservoirs of infection in gibbons may hamper ongoing attempts at permanent eradication of HBV infection from human populations in South-East Asia through immunization.
Sall
A A
AA
Institut Pasteur du Cambodge, 5 Boulevard Monivong, Phnom Penh, Cambodia.
Starkman
S
S
Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK.
Reynes
J M
JM
Institut Pasteur du Cambodge, 5 Boulevard Monivong, Phnom Penh, Cambodia.
Lay
S
S
Institut Pasteur du Cambodge, 5 Boulevard Monivong, Phnom Penh, Cambodia.
Nhim
T
T
Phnom Tamao Wildlife Rescue Center, Takeo Province, Cambodia.
Hunt
M
M
WildAid Cambodia, Villa 109, Street 99, Chamcarmon District, Phnom Penh, Cambodia.
Marx
N
N
WildAid Cambodia, Villa 109, Street 99, Chamcarmon District, Phnom Penh, Cambodia.
Simmonds
P
P
Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK.
D000818:Animals; D002160:Cambodia / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D004197:Disease Reservoirs / Q000662:veterinary*; D006509:Hepatitis B / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006922:Hylobates / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D013045:Species Specificity
D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens