[Simultaneous determination of 4 anthraquinones in Kuhuang injection by RP-HPLC].
OBJECTIVE
To develop a new high-performance liquid chromatographic method for simultaneous quantitative determination of 4 anthraquinones in Kuhuang injection.


METHODS
The chromatographic separation was performed on a Lichrospher C18 column (4.6 mm x 250 mm, 5 microm,), A linear gradient elution of A (CH3COOH: H2O = 1:100) and B (CH3COOH: H2O: CH3CN = 1:20:80) was used. The flow rate was 0.8 mL x min(-1) and column temperature was set at 35 degrees C. The UV detection wavelength was set at 254 nm.


RESULTS
The recoveries of rhein, emodin, chrysophanol and physcion were 98.9%, 100.5%, 102.5% and 99.0%, respectively. Their linear ranges were 0.0875-1.75 microg for rhein, 0.0825-1.65 microg for emodin, 0.159-3.17 microg for chrysophanol and 0.0525-1.05 microg for physcion, respectively.


CONCLUSIONS
The method was accurate, repeatable and suitable to determine the contents of 4 anthraquinones in Kuhuang injection.
Wu
Yong-jiang
YJ
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027.
Chen
Jian-jun
JJ
Cheng
Yi-yu
YY
D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D004642:Emodin / Q000031:analogs & derivatives* / Q000032:analysis; D007267:Injections; D010946:Plants, Medicinal / Q000737:chemistry*; D012250:Rheum / Q000737:chemistry; D029909:Sophora / Q000737:chemistry
D000880:Anthraquinones; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid; C020491:rhein