Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples.
A polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. Phenotypically these isolates shared several characteristics with members of the Alcaligenaceae and related genera. Random amplified polymorphic DNA fingerprinting and whole-cell protein analysis suggested the presence of multiple genomic groups, which was confirmed by DNA-DNA hybridization experiments. 16S rRNA gene sequence analysis indicated that these isolates were related to the genera Pelistega, Taylorella, Oligella, Pigmentiphaga, Alcaligenes, Kerstersia, Achromobacter and Bordetella and belonged to the family Alcaligenaceae. Based on the results of the present study the organisms were classified in a novel genus, Advenella gen. nov. This genus comprises one named species, Advenella incenata sp. nov. (type strain LMG 22250T=CCUG 45225T) and five currently unnamed genomic species. The DNA G+C content of members of the novel genus Advenella is between 54.0 and 57.7 mol%.
Coenye
Tom
T
Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium.
Vanlaere
Elke
E
Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium.
Samyn
Emly
E
Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium.
Falsen
Enevold
E
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
Larsson
Peter
P
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
Vandamme
Peter
P
Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium.
D000293:Adolescent; D000328:Adult; D042401:Alcaligenaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D003550:Cystic Fibrosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology* / Q000662:veterinary; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S