title: Rapid method to screen resistance of potato plants against Myzus persicae (Homoptera: Aphididae) in the laboratory.
abstract: With the objective to develop a potato, Solanum tuberosum L., resistance program against aphids, we propose a rapid screening method with Myzus persicae (Sulzer) in the laboratory. We aimed to optimize the duration of the whole procedure and to decrease the frequency of measurements. In a first experiment, intrinsic rate of natural increase (r(m)) values were compared between adult aphids reared throughout their entire life and adults reared only during a period equivalent to their prereproductive period. No significant differences were observed. In a second experiment, four groups of aphids were distinguished according to the sampling frequency, i.e., those whose biological parameters were evaluated every single, second, third, and fourth day. Except for the fourth-day experiment, the r(m) values estimated on aphids reared on the three potato lines were not significantly different whatever sampling frequency of single, second, or third day used to check aphids. Thus, screening efforts in laboratory can be largely optimized by evaluating adult aphids only during a period equivalent to their prereproductive period and assessing M. persicae populations every third day. Our method is reliable and adapted to screen a large number of potato plants against M. persicae because it allows an average 70% reduction in the time required for the whole experimental process.
authors.affiliation: Biologie des Entomophages (UPRES EA 2084-2085), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Le Roux
authors.forename: Julien
authors.initials: J
authors.lastname: Saguez
authors.forename: Charles
authors.initials: C
authors.lastname: Vincent
authors.forename: Philippe
authors.initials: P
authors.lastname: Giordanengo
mesh_terms: D000818:Animals; D001042:Aphids / Q000254:growth & development*; D002688:Chitinases / Q000235:genetics; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics; D013997:Time Factors
chemical_list: D002688:Chitinases