title: Field gradient electrophoresis.
abstract: The class of equilibrium gradient methods utilizes the opposition of two forces, at least one of which changes in magnitude with position, to separate and concentrate analytes. The drawback of many methods of this type is that the production of two opposing forces requires in comparison to standard methods, such as capillary electrophoresis, a relatively complex apparatus. In addition, for techniques such as electric field gradient focusing, hydrodynamic flow leads to Taylor dispersion, which limits the attainable concentration factor. We propose a new method, gradient field electrophoresis, which achieves analyte separation and focusing with only one spatially varying force, an electric field gradient. A model for the method is developed and used to analyze peak capacity. Experimental results for a protein (R-phycoerythrin) are given and compared to the model.
authors.affiliation: Department of Electrical and Computer Engineering, Brigham Young University, Provo, UT 84602, USA. warnick@ee.byu.edu
authors.forename: Karl F
authors.initials: KF
authors.lastname: Warnick
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Francom
authors.forename: Paul H
authors.initials: PH
authors.lastname: Humble
authors.forename: Ryan T
authors.initials: RT
authors.lastname: Kelly
authors.forename: Adam T
authors.initials: AT
authors.lastname: Woolley
authors.forename: Milton L
authors.initials: ML
authors.lastname: Lee
authors.forename: H Dennis
authors.initials: HD
authors.lastname: Tolley
mesh_terms: D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D007525:Isoelectric Focusing; D008962:Models, Theoretical; D010799:Phycoerythrin / Q000302:isolation & purification; D055672:Static Electricity
chemical_list: D010799:Phycoerythrin