title: Evaluation of pulsed Doppler tissue velocity imaging for assessing systolic function of murine global heart failure.
abstract: The feasibility of Doppler tissue imaging (DTI) for assessing global systolic function has not been determined in small animals, particularly at near-conscious heart rates. Therefore, we compared DTI measurements with conventional M-mode-derived fractional shortening in murine global left ventricular systolic dysfunction induced by intraperitoneal doxorubicin (Dox) injection. In all, 13 female C57BL mice received 20 mg/kg of Dox and 12 mice received saline injection (controls). DTI signals were obtained from the inferior wall through parasternal short-axis views. The heart rate was kept at near-conscious level throughout DTI measurements (approximately 500/min). Left ventricular systolic dysfunction was detectable by measurements of fractional shortening from 4 to 14 days after Dox administration. Among DTI measurements, peak systolic velocity and time to peak systolic velocity decreased from 4 to 14 days after Dox injection. Our results indicate that these new DTI measurements appear feasible to assess global left ventricular systolic dysfunction in mice.
authors.affiliation: Cardiovascular Branch, National Heart, Lung, and Blood Institute/NIH, Building 10/7B15, 10 Center Drive, MSC-1650, Bethesda, MD 20892, USA.
authors.forename: Satoaki
authors.initials: S
authors.lastname: Matoba
authors.forename: Paul M
authors.initials: PM
authors.lastname: Hwang
authors.forename: Tammy
authors.initials: T
authors.lastname: Nguyen
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Shizukuda
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic; D001783:Blood Flow Velocity / Q000187:drug effects; D004195:Disease Models, Animal; D004317:Doxorubicin; D018619:Echocardiography, Doppler, Pulsed; D005260:Female; D006333:Heart Failure / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology; D015588:Observer Variation; D015203:Reproducibility of Results; D013318:Stroke Volume / Q000187:drug effects; D013599:Systole / Q000187:drug effects; D018487:Ventricular Dysfunction, Left / Q000000981:diagnostic imaging* / Q000503:physiopathology*
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin