title: Growth kinetics of extremely halophilic archaea (family halobacteriaceae) as revealed by arrhenius plots.
abstract: Members of the family Halobacteriaceae in the domain Archaea are obligate extreme halophiles. They occupy a variety of hypersaline environments, and their cellular biochemistry functions in a nearly saturated salty milieu. Despite extensive study, a detailed analysis of their growth kinetics is missing. To remedy this, Arrhenius plots for 14 type species of the family were generated. These organisms had maximum growth temperatures ranging from 49 to 58 degrees C. Nine of the organisms exhibited a single temperature optimum, while five grew optimally at more than one temperature. Generation times at these optimal temperatures ranged from 1.5 h (Haloterrigena turkmenica) to 3.0 h (Haloarcula vallismortis and Halorubrum saccharovorum). All shared an inflection point at 31 +/- 4 degrees C, and the temperature characteristics for 12 of the 14 type species were nearly parallel. The other two species (Natronomonas pharaonis and Natronorubrum bangense) had significantly different temperature characteristics, suggesting that the physiology of these strains is different. In addition, these data show that the type species for the family Halobacteriaceae share similar growth kinetics and are capable of much faster growth at higher temperatures than those previously reported.
authors.affiliation: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640, USA.
authors.forename: Jessie L
authors.initials: JL
authors.lastname: Robinson
authors.forename: Brandy
authors.initials: B
authors.lastname: Pyzyna
authors.forename: Rachelle G
authors.initials: RG
authors.lastname: Atrasz
authors.forename: Christine A
authors.initials: CA
authors.lastname: Henderson
authors.forename: Kira L
authors.initials: KL
authors.lastname: Morrill
authors.forename: Anna Mae
authors.initials: AM
authors.lastname: Burd
authors.forename: Erik
authors.initials: E
authors.lastname: Desoucy
authors.forename: Rex E
authors.initials: RE
authors.lastname: Fogleman
authors.forename: John B
authors.initials: JB
authors.lastname: Naylor
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Steele
authors.forename: Dawn R
authors.initials: DR
authors.lastname: Elliott
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Leyva
authors.forename: Richard F
authors.initials: RF
authors.lastname: Shand
mesh_terms: D006216:Halobacteriaceae / Q000145:classification / Q000254:growth & development*; D006358:Hot Temperature; D007700:Kinetics; D009994:Osmolar Concentration; D012965:Sodium Chloride; D013816:Thermodynamics
chemical_list: D012965:Sodium Chloride