title: Civets are equally susceptible to experimental infection by two different severe acute respiratory syndrome coronavirus isolates.
abstract: Severe acute respiratory syndrome (SARS) was caused by a novel virus now known as SARS coronavirus (SARS-CoV). The discovery of SARS-CoV-like viruses in masked palm civets (Paguma larvata) raises the possibility that civets play a role in SARS-CoV transmission. To test the susceptibility of civets to experimental infection by different SARS-CoV isolates, 10 civets were inoculated with two human isolates of SARS-CoV, BJ01 (with a 29-nucleotide deletion) and GZ01 (without the 29-nucleotide deletion). All inoculated animals displayed clinical symptoms, such as fever, lethargy, and loss of aggressiveness, and the infection was confirmed by virus isolation, detection of viral genomic RNA, and serum-neutralizing antibodies. Our data show that civets were equally susceptible to SARS-CoV isolates GZ01 and BJ01.
authors.affiliation: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 150001, China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Wu
authors.forename: Changchun
authors.initials: C
authors.lastname: Tu
authors.forename: Chaoan
authors.initials: C
authors.lastname: Xin
authors.forename: Hua
authors.initials: H
authors.lastname: Xuan
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Meng
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yedong
authors.initials: Y
authors.lastname: Yu
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Guan
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xunnan
authors.initials: X
authors.lastname: Yin
authors.forename: Gary
authors.initials: G
authors.lastname: Crameri
authors.forename: Muping
authors.initials: M
authors.lastname: Wang
authors.forename: Changwen
authors.initials: C
authors.lastname: Li
authors.forename: Shengwang
authors.initials: S
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Hua
authors.initials: H
authors.lastname: Xiang
authors.forename: Jinfu
authors.initials: J
authors.lastname: Sun
authors.forename: Jinding
authors.initials: J
authors.lastname: Chen
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Sun
authors.forename: Shoulin
authors.initials: S
authors.lastname: Gu
authors.forename: Nihong
authors.initials: N
authors.lastname: Liu
authors.forename: Dexia
authors.initials: D
authors.lastname: Fu
authors.forename: Bryan T
authors.initials: BT
authors.lastname: Eaton
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
authors.forename: Xiangang
authors.initials: X
authors.lastname: Kong
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000503:physiopathology* / Q000635:transmission; D002415:Cats; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000503:physiopathology* / Q000517:prevention & control / Q000821:virology; D045949:Viverridae