The 3-hydroxy group and 4,5-trans double bond of sphingomyelin are essential for modulation of galactosylceramide transmembrane asymmetry.
The structural features of SPM that control the transbilayer distribution of beta-GalCer in POPC vesicles were investigated by (13)C- and (31)P-NMR spectroscopy using lipid analogs that share physical similarities with GalCer or SPM. The SPM analogs included N-palmitoyl-4,5-dihydro-SPM, 3-deoxy-SPM, 1-alkyl-2-amidophosphatidylcholine, and dipalmitoylphosphatidylcholine, a popular model "raft lipid". The transbilayer distributions of the SPM analogs and SPM in POPC vesicles were similar by (31)P-NMR. To observe the dramatic change in GalCer transbilayer distribution that occurs when SPM is included in POPC vesicles, the 3-OH group, 4,5-trans double bond, and amide linkage all were required in SPM. However, inclusion of 2 and 10 mol % dihydroSPM in SPM/POPC (1:1) vesicles mitigated and completely abrogated the effect of SPM on the transbilayer distribution of GalCer. Despite sharing some structural features with GalCer and localizing preferentially to the inner leaflet of POPC vesicles, dimyristoylphosphatidylethanolamine did not undergo a change in transbilayer distribution when SPM was incorporated into the vesicles. The results support the hypothesis that specific interactions may be favored among select sphingolipids in curvature-stressed membranes and emphasize the potential importance of the SPM-dihydroSPM ratio in membrane fission and fusion processes associated with vesicle biogenesis and trafficking.
Malewicz
Barbara
B
University of Minnesota, Hormel Institute, Austin, Minnesota 55912, USA.
Valiyaveettil
Jacob T
JT
Jacob
Kochurani
K
Byun
Hoe-Sup
HS
Mattjus
Peter
P
Baumann
Wolfgang J
WJ
Bittman
Robert
R
Brown
Rhoderick E
RE
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000818:Animals; D001703:Biophysics / Q000379:methods*; D002645:Chickens; D005699:Galactosylceramides / Q000737:chemistry*; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D021962:Membrane Microdomains / Q000737:chemistry; D008958:Models, Molecular; D009584:Nitrogen / Q000737:chemistry; D010063:Ovum; D010100:Oxygen / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D013107:Sphingolipids / Q000737:chemistry; D013109:Sphingomyelins / Q000737:chemistry*
D005699:Galactosylceramides; D008051:Lipid Bilayers; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D013107:Sphingolipids; D013109:Sphingomyelins; D015060:1,2-Dipalmitoylphosphatidylcholine; D009584:Nitrogen; D010100:Oxygen; C028694:1-palmitoyl-2-oleoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine