Comparative effect of the fungicide Prochloraz-Mn on Agaricus bisporus vegetative-mycelium and fruit-body cell walls.
Fungicides to control mycopathogens of commercial Agaricus bisporus, a mushroom cultivated for human consumption, are a major field of study, since these chemicals are toxic to both the host and its fungal parasites. The fungicide Prochloraz-Mn, used at its LD50 for A. bisporus, partially inhibited protein biosynthesis in the vegetative mycelial cell walls of this mushroom and caused significant changes in cell-wall polysaccharide structure, as deduced by methylation analysis and gas liquid chromatography-mass spectrometry (GLC-MS). Furthermore, the aggregated mycelial walls showed distinct alterations in their overall chemical composition following the administration of Prochloraz-Mn at the LD50 and the LD50 x1000. As expected, GLC-MS studies indicated that the latter dose caused more appreciable differences in polysaccharide structure. The decrease in mushroom crop yields obtained from industrial cultures treated with Prochloraz-Mn to control V. fungicola infection depended on the dose of the fungicide employed, whereas fruit-body morphology was only slightly affected at the highest Prochloraz-Mn concentration used.
Bernardo
Dolores
D
Biological Research Center, CSIC, Madrid, Spain.
Pérez Cabo
Amelia
A
Novaes-Ledieu
Monique
M
Pardo
José
J
García Mendoza
Concepción
C
D000364:Agaricus / Q000187:drug effects* / Q000254:growth & development; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000187:drug effects* / Q000254:growth & development; D005659:Fungicides, Industrial / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D007093:Imidazoles / Q000494:pharmacology*; D025282:Mycelium / Q000187:drug effects* / Q000254:growth & development; D011134:Polysaccharides / Q000378:metabolism
D005659:Fungicides, Industrial; D007093:Imidazoles; D011134:Polysaccharides; C045362:prochloraz