A repetitive movement detector used for automatic monitoring and quantification of scratching in mice.
We have designed an economical non-invasive movement detector for small animal studies and used it for monitoring and quantifying itch in mice. The system is based on a sensitive force transducer positioned below a recording platform holding a lightweight polystyrene recording box in which an animal is placed. A programmed micro-controller is used to discriminate between non-specific movement, grooming behaviour, and scratching movements made by the animal's hind limb. Following sub-dermal injection of histamine receptor agonists into the neck of a mouse, dose-related scratching occurred which was detected and quantified. There was 91% correlation between bouts of scratching as counted manually from playback of the video recording and recorded by the detector. The detector was also able rapidly to count the individual scratch movements of the hind limb that comprise a bout, with 95% accuracy in comparison with manual counting during slow motion playback of video tape, something that is impossible for an unaided observer to achieve because individual scratch movements are too fast to discriminate by eye. Separate detectors were used for the efficient non-invasive study of four animals simultaneously, and this number could easily be increased by adding more platforms. The system could also be modified to record the animal's position within the box, which would be of value in studies involving exploratory behaviour. In summary, the non-invasive multichannel repetitive movement detector will be very useful for accurate measurement of scratching during pruritus studies in small animals, with considerable savings in staff time and effort. It should therefore be a valuable tool for helping to investigate pruritus and in the evaluation of anti-pruritic drugs.
Brash
H M
HM
Department of Hepatology, University of Edinburgh, Royal Infirmary of Edinburgh, Edinburgh EH16 4SA, UK. hmb@staffmail.ed.ac.uk
McQueen
D S
DS
Christie
D
D
Bell
J K
JK
Bond
S M
SM
Rees
J L
JL
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001525:Behavioral Sciences / Q000295:instrumentation* / Q000379:methods; D004581:Electronics / Q000295:instrumentation* / Q000379:methods; D005121:Extremities / Q000502:physiology; D005260:Female; D017442:Histamine Agonists / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009068:Movement / Q000502:physiology*; D011537:Pruritus / Q000503:physiopathology*; D011968:Receptors, Histamine / Q000187:drug effects / Q000502:physiology; D014741:Video Recording / Q000295:instrumentation / Q000379:methods
D017442:Histamine Agonists; D011968:Receptors, Histamine