Suppressive effects of a water extract of Ulmus davidiana Planch (Ulmaceae) on collagen-induced arthritis in mice.
Ulmus davidiana Planch (Ulmaceae) has long been known to have anti-inflammatory and protective effects on damaged tissue, inflammation and bone resorption. Therefore, this study was undertaken to address (1) whether the water extract of the bark of Ulmus davidiana Planch (Ulmaceae) (UD) can modulate the expression of inducible inflammatory cytokines in mice; (2) in order to assess the therapeutic effects of UD in collagen-induced arthritis (CIA) in mice. DBA/1 mice were immunized with bovine type II collagen. After a second collagen immunization, mice were treated with UD orally at 100mg/kg once a day for 3 weeks. Paws were evaluated macroscopically for redness, swelling and deformities. The levels of TNF-alpha and IL-1beta in the ankle were examined. The severity of arthritis within the knee joints was evaluated by histological assessment of cartilage destruction and pannus formation. Administration of UD significantly suppressed the progression of CIA and inhibited the production of TNF-alpha and IL-1beta in the paws. The erosion of cartilage was dramatically reduced in mouse knees after treatment with UD. In the serum of UD-treated mice, the levels of IL-4 and IL-10, anti-inflammatory cytokines, were increased. From the results, it was concluded that administration of UD has therapeutic effects on CIA including protection of cartilage and RA for a potential therapy.
Kim
Kap-Sung
KS
Department of Acupuncture, Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University and National Research Laboratory for Glycobiology, Kyungju, Kyungbuk 780-714, Korea.
Lee
Seung-Duk
SD
Kim
Kyung-Ho
KH
Kil
Sang-Yong
SY
Chung
Kang-Hyun
KH
Kim
Cheorl-Ho
CH
D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D007166:Immunosuppressive Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D007378:Interleukins / Q000097:blood; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use; D018547:Plant Stems; D051381:Rats; D017207:Rats, Sprague-Dawley; D034403:Ulmus; D014867:Water / Q000494:pharmacology
D007166:Immunosuppressive Agents; D007378:Interleukins; D010936:Plant Extracts; D014867:Water