[Mammalian carcinogen-induced warts tumor in Drosophilidae: a test sensitive to the blastomogenic action of chemical compounds].
A basically new system has been developed to screen carcinogens in Drosophilidae, which is based on somatic mutagenesis and recombination. The system may induce tumors in Drosophilidae, which are recorded in adult insects. The test uses recessive mutation in the suppressor gene of growth of warts (wts) tumors. The new system is sensitive to a wide spectrum of mutagenic carcinogens that are naturally encountered. The sensitivity of the system to polycyclic aromatic hydrocarbons and aromatic amides is higher than that of classical tests. Dominant p53 gene mutation that ceases mutagen-induced apoptosis has been shown to increase the incidence of wts tumors by many times. The magnitude of the effect depends on the rate of mutant p53 expression. The increasing effect of p53 mutation extends to both somatic recombination and point mutations and deletions at the wts locus.
Sidorov
R A
RA
Belitskiĭ
G A
GA
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D017209:Apoptosis; D001564:Benzo(a)pyrene / Q000633:toxicity; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000633:toxicity; D003627:Data Interpretation, Statistical; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D029721:Drosophila Proteins; D018428:Drosophilidae / Q000187:drug effects* / Q000235:genetics*; D005260:Female; D016153:Genes, Suppressor; D016158:Genes, p53; D005838:Genotype; D006579:Heterozygote; D008297:Male; D009154:Mutation; D017354:Point Mutation; D011494:Protein Kinases; D012680:Sensitivity and Specificity; D014860:Warts / Q000235:genetics
D000970:Antineoplastic Agents; D002273:Carcinogens; D029721:Drosophila Proteins; D001564:Benzo(a)pyrene; D011494:Protein Kinases; C093503:wts protein, Drosophila; D002945:Cisplatin; C043804:diamminedichlorodihydroxyplatinum IV; D004121:Dimethyl Sulfoxide