In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.
DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular domain of DivIB was found to associate with a heterodimer of those of DivIC and FtsL. The heterodimerization of DivIC and FtsL was artificially constrained by fusion with interacting coiled-coils. Immunofluorescence experiments showed that DivIC is always localized at mid-cell, in contrast to DivIB and FtsL, which are co-localized with DivIC only during septation. Taken together, our data suggest that assembly of the trimeric complex DivIB/DivIC/FtsL is regulated during the cell cycle through controlled formation of the DivIC/FtsL heterodimer.
Noirclerc-Savoye
Marjolaine
M
Laboratoire d'Ingénierie des Macromolécules, Institut de Biologie Structurale (CEA/CNRS UMR 5075/UJF), 41 rue Jules Horowitz, 38027 Grenoble, France.
Le Gouëllec
Audrey
A
Morlot
Cécile
C
Dideberg
Otto
O
Vernet
Thierry
T
Zapun
André
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D019281:Dimerization; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013296:Streptococcus pneumoniae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C085861:DivIB protein, Bacillus subtilis; C110609:DivIC protein, Bacillus subtilis; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C078387:ftsL protein, E coli