title: Collecting baseline corticosterone samples in the field: is under 3 min good enough?
abstract: Evaluating corticosterone (CORT) responses to stress in free-living vertebrates requires knowing the unstressed titers prior to capture. Based upon laboratory data, the assumption has been that samples collected in less than 3 min of capture will reflect these unstressed concentrations. This assumption was tested for six species using samples collected from 945 individuals at 0-6 min after capture. Samples were from five avian species trapped at multiple times of year and one reptilian species, comprising a total of 14 different data sets for comparisons. For seven of 14 data sets, including five species, there was no significant increase in corticosterone titers within 3 min of capture. In six of the 14 data sets, corticosterone titers increased significantly after 2 min, and in one data set, the increase started at 1.5 min. In all seven of the cases showing an increase before 3 min, however, corticosterone titers from the time of increase to 3 min were significantly lower than titers after 30 min of restraint stress. These results indicate a high degree of confidence for these species that samples collected in less than 2 min reflect unstressed (baseline) concentrations, and that samples collected from 2-3 min also will likely reflect baseline concentrations but at worst are near baseline.
authors.affiliation: Department of Biology, Tufts University, Medford, MA 02155, USA. michael.romero@tufts.edu
authors.forename: L Michael
authors.initials: LM
authors.lastname: Romero
authors.forename: J Michael
authors.initials: JM
authors.lastname: Reed
mesh_terms: D000818:Animals; D001717:Birds / Q000097:blood* / Q000502:physiology; D003345:Corticosterone / Q000097:blood*; D004704:Endocrinology / Q000379:methods; D007076:Iguanas / Q000097:blood* / Q000502:physiology; D013315:Stress, Psychological / Q000097:blood*; D013997:Time Factors
chemical_list: D003345:Corticosterone