TNF gene polymorphisms and Helicobacter Pylori infection in gastric carcinogenesis in Chinese population.
OBJECTIVE
Helicobacter pylori (H. pylori) is a major cause of chronic gastritis and peptic ulcer disease, and a definite carcinogen for gastric adenocarcinoma. However, the underlying pathogenic mechanisms have not been fully understood although the interactions between environmental, bacterial, and multiple genetic components are likely to be involved. Tumor necrosis factor (TNF) is a key cytokine involved in H. pylori-induced gastric inflammation. The present study aimed to determine the di-allelic polymorphisms of TNF gene and their association with H. pylori infection and gastroduodenal diseases in Chinese population of Han nationality.


METHODS
Two hundred and ten patients with gastroduodenal diseases (73 chronic gastritis, 78 duodenal ulcer, and 59 noncardia gastric cancer) and 264 healthy controls were genotyped by the PCR-RFLP method for TNF-alpha 308, lymphotoxin-alpha (LT-alpha) NcoI, and AspHI gene polymorphisms. H. pylori infection status was determined by a validated serological test.


RESULTS
H. pylori infection was detected in 90.5% of 210 patients and 62.1% of 264 healthy controls (p < 0.0001; odds ratio [OR]= 5.793; 95%CI: 3.431-9.780). Frequency of LT-alphaNcoI A/G genotype in patients with noncardia gastric cancer with H. pylori infection was significantly higher than that in H. pylori-positive healthy controls (64.0%vs 46.0%; p= 0.0297; OR = 2.026; 95%CI: 1.080-3.803). There were no other associations between TNF-alpha 308, LT-alphaNcoI, and AspHI gene polymorphisms and H. pylori infection in gastroduodenal diseases.


CONCLUSIONS
LT-alphaNcoI A/G heterozygous genotype was associated with H. pylori infection in patients with noncardia gastric cancer in Chinese Han population.
Li
Chun
C
Department of Internal Medicine, Wuhan University Zhongnan Hospital, Donghu Road 169, Wuhan 430 071, People's Republic of China.
Xia
Bing
B
Yang
Yi
Y
Li
Jin
J
Xia
Harry Hua-Xiang
HH
D002681:China / Q000453:epidemiology; D002908:Chronic Disease; D004381:Duodenal Ulcer / Q000382:microbiology; D005260:Female; D005756:Gastritis / Q000382:microbiology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000150:complications* / Q000453:epidemiology; D016480:Helicobacter pylori; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D015995:Prevalence; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha