Valproic acid induces apoptosis dependent of Yca1p at concentrations that mildly affect the proliferation of yeast.
Valproic acid (VPA) inhibited the growth of yeast in a dose-dependent manner with complete inhibition attained at 100 mM. When cells were exposed to 25 mM VPA, the wild-type died showing apoptotic markers, while yca1Delta deleted of YCA1 encoding yeast caspase 1 survived. On the other hand, when cells were exposed to 50 mM VPA, both the wild-type and yca1Delta died showing morphological features similar to those of the autophagic death of cdc28 which was also independent of YCA1. Thus, these results suggested that yeast cells die via YCA1-dependent apoptosis when their proliferative activity is mildly impaired.
Mitsui
Kazuhiro
K
University of Yamanashi, Faculty of Medicine, Department of Biochemistry 2, 1110 Shimokato, Tamaho, Nakakoma, Yamanashi 409-3898, Japan.
Nakagawa
Daichi
D
Nakamura
Masahiro
M
Okamoto
Taro
T
Tsurugi
Kunio
K
D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy; D015415:Biomarkers; D049109:Cell Proliferation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D014635:Valproic Acid / Q000494:pharmacology*
D015415:Biomarkers; D029701:Saccharomyces cerevisiae Proteins; D014635:Valproic Acid