title: Insect-resistant transgenic Pinus radiata.
abstract: Transgenic radiata pine (Pinus radiata D. Don) plants containing a Bacillus thuringiensis (Bt) toxin gene, crylAc, were produced by means of biolistic transformation of embryogenic tissue. Using the selectable marker gene nptII and corresponding geneticin selection, 20 independent transgenic lines from five genotypes were established. Over 200 plants regenerated from ten transgenic lines were successfully transferred to soil. The integration and expression of the introduced genes in transgenic tissue and/or plants were confirmed by PCR, Southern hybridisation and neomycin phosphotransferase II (NPTII) and Bt ELISA assays. Bioassays with larvae of the painted apple moth, Teia anartoides, demonstrated that transgenic plants displayed variable levels of resistance to insect damage, with one transgenic line being highly resistant to feeding damage.
authors.affiliation: New Zealand Forest Research Institute Ltd, Private Bag 3020, Rotorua, New Zealand. lynette.grace@forestresearch.co.nz
authors.forename: Lynette J
authors.initials: LJ
authors.lastname: Grace
authors.forename: Julia A
authors.initials: JA
authors.lastname: Charity
authors.forename: Belinda
authors.initials: B
authors.lastname: Gresham
authors.forename: Nod
authors.initials: N
authors.lastname: Kay
authors.forename: Christian
authors.initials: C
authors.lastname: Walter
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D001681:Biological Assay; D004731:Endotoxins / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005838:Genotype; D006460:Hemolysin Proteins; D007313:Insecta / Q000502:physiology*; D007814:Larva / Q000502:physiology; D009036:Moths / Q000502:physiology; D010572:Pest Control, Biological / Q000379:methods*; D028223:Pinus / Q000235:genetics* / Q000469:parasitology; D030821:Plants, Genetically Modified / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D005819:Genetic Markers; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis