Occurrence of the strA-strB streptomycin resistance genes in Pseudomonas species isolated from kiwifruit plants.
The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA-->CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gln to Arg.
Han
Hyo Shim
HS
Department of Biology, Sunchon National University, Sunchon 540-742, Republic of Korea.
Koh
Young Jin
YJ
Hur
Jae-Seoun
JS
Jung
Jae Sung
JS
D029042:Actinidia / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D007564:Japan; D007723:Korea; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D017421:Sequence Analysis; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D013307:Streptomycin