The biocide triclosan selects Stenotrophomonas maltophilia mutants that overproduce the SmeDEF multidrug efflux pump.
The possibility that triclosan selects Stenotrophomonas maltophilia mutants overexpressing the multidrug resistance pump SmeDEF is analyzed. Five out of 12 triclosan-selected mutants were less susceptible to antibiotics than the wild-type strain and overproduced SmeDEF. Results are discussed in relation to current debates on the potential selection of antibiotic-resistant bacteria by household biocides.
Sanchez
Patricia
P
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Moreno
Eduardo
E
Martinez
Jose L
JL
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D026901:Membrane Transport Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000235:genetics*; D014260:Triclosan / Q000494:pharmacology*
D000891:Anti-Infective Agents, Local; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C417265:SmeD protein, Stenotrophomonas maltophilia; C417266:SmeE protein, Stenotrophomonas maltophilia; C417267:SmeF protein, Stenotrophomonas maltophilia; D014260:Triclosan