Age-specific threats induce CRF expression in the paraventricular nucleus of the hypothalamus and hippocampus of young rats.
Young animals respond to threatening stimuli in an age-specific way. Their endocrine and behavioral responses reflect the potential threat of the situation at a given age. The aim of the present study was to determine whether corticotropin-releasing factor (CRF) is involved in the endocrine and behavioral responses to threat and their developmental changes in young rats. Preweaning 14-day-old and postweaning 26-day-old rats were exposed to two age-specific threats, cat odor and an adult male rat. The acute behavioral response was determined during exposure. After exposure, the time courses of the corticosterone response and of CRF expression in the paraventricular nucleus of the hypothalamus (PVN) and in extrahypothalamic areas were assessed. Preweaning rats became immobile when exposed to cat odor or the male rat, whereas postweaning rats became immobile to cat odor only. Male exposure increased serum corticosterone levels in 14-day-old rats, but cat odor failed to increase levels at either age. Exposure induced elevation of CRF mRNA levels in the PVN that paralleled changes in corticosterone levels. CRF may thus play a role in endocrine regulation and its developmental changes during early life. Neither cat odor nor the adult male altered CRF mRNA levels in the bed nucleus of the stria terminalis (BNST) or the amygdala, but both stimuli increased levels in the hippocampus. Hippocampal CRF mRNA expression levels did not parallel cat odor or male-induced immobility, indicating that CRF is not involved in this response in young rats but may be involved in aspects of learning and memory.
Wiedenmayer
Christoph P
CP
Department of Psychiatry, Columbia University, New York, NY 10032, USA. cpw14@columbia.edu
Magariños
Ana M
AM
McEwen
Bruce S
BS
Barr
Gordon A
GA
D000367:Age Factors; D000679:Amygdala / Q000254:growth & development / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D002415:Cats; D003345:Corticosterone / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D005239:Fear / Q000502:physiology*; D005260:Female; D006624:Hippocampus / Q000254:growth & development / Q000502:physiology*; D008297:Male; D009812:Odorants; D010286:Paraventricular Hypothalamic Nucleus / Q000254:growth & development / Q000502:physiology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020318:Rats, Long-Evans
D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone