title: Monitoring expression of cytochrome P450 genes during postischemic rat liver reperfusion using DNA microarrays.
abstract: We investigated the expression of 50 cytochrome P450 (CYP) genes in the postischemic reperfused rat liver using a DNA microarray. Thirteen CYPs showed absent expression in all experiments and 2 CYPs were induced by pentobarbital, which was used as an anesthetic. Eight of the remaining 35 CYPs showed significantly decreased expression following ischemia-reperfusion. Monitoring CYP expression may be a powerful approach for elucidation of pathways regulating drug metabolism that may be involved in postischemic reperfusion liver injury.
authors.affiliation: Division of Genomic Epidemiology & Clinical Trials, Advanced Medical Research Center, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Nishida
authors.forename: Yukimoto
authors.initials: Y
authors.lastname: Ishii
authors.forename: Koichi
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Asai
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000098:blood supply*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010634:Phenobarbital / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013250:Steroid Hydroxylases / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
chemical_list: D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D019362:Cytochrome P-450 CYP2B1; C025619:steroid 16-beta-hydroxylase; D010634:Phenobarbital