title: A mRNA PCR for the diagnosis of feline infectious peritonitis.
abstract: A reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of feline coronavirus (FCoV) messenger RNA in peripheral blood mononuclear cells (PBMCs) is described. The assay is evaluated as a diagnostic test for feline infectious peritonitis (FIP). It is based on a well-documented key event in the development of FIP: the replication of virulent FCoV mutants in monocytes/macrophages. To detect most feline coronavirus field strains, the test was designed to amplify subgenomic mRNA of the highly conserved M gene. The test was applied to 1075 feline blood samples (424 from healthy, 651 from sick cats suspected of FIP) and returned 46% of the diseased cats as positive for feline coronavirus mRNA in their peripheral blood cells; of the healthy cats, 5% tested positive. Of a group of 81 animals in which FIP had been confirmed by post-mortem examination, 75 (93%) tested positive, whereas 17 cats with different pathologies (non-FIP cases) all tested negative. In view of the low rate of false-positive results (high specificity) the mRNA RT-PCR may be a valuable addition to the diagnostic arsenal for FIP.
authors.affiliation: Institute of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands. f.simmons1@janbe.jnj.com
authors.forename: Fermin A
authors.initials: FA
authors.lastname: Simons
authors.forename: Harry
authors.initials: H
authors.lastname: Vennema
authors.forename: Jaime E
authors.initials: JE
authors.lastname: Rofina
authors.forename: Jan M
authors.initials: JM
authors.lastname: Pol
authors.forename: Marian C
authors.initials: MC
authors.lastname: Horzinek
authors.forename: Peter J M
authors.initials: PJ
authors.lastname: Rottier
authors.forename: Herman F
authors.initials: HF
authors.lastname: Egberink
mesh_terms: D000818:Animals; D002415:Cats; D016765:Coronavirus, Feline / Q000302:isolation & purification*; D016766:Feline Infectious Peritonitis / Q000175:diagnosis*; D012333:RNA, Messenger / Q000097:blood*; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral