Microarrays, antiobesity and the liver.
In this review, the microarray technology and especially oligonucleotide arrays are exemplified with a practical example taken from the perilipin-/- mice and using the dChip software, available for non-lucrative purposes. It was found that the liver of perilipin-/- mice was healthy and normal, even under high-fat diet when compared with the results published for the scd1-/- mice, which under high-fat diets had a darker liver, suggestive of hepatic steatosis. Scd1 is required for the biosynthesis of monounsaturated fatty acids and plays a key role in the hepatic synthesis of triglycerides and of very-low-density lipoproteins. Both models of obesity resistance share many similar phenotypic antiobesity features, however, the perilipin-/- mice had a significant downregulation of stearoyl CoA desaturases scd1 and scd2 in its white adipose tissue, but a normal level of both genes inside the liver, even under high-fat diet. Here, different microarray methodologies are discussed, and also some of the most recent discoveries and perspectives regarding the use of microarrays, with an emphasis on obesity gene expression, and a personal remark on my findings of increased expression for hemoglobin transcripts and other hemo related genes (hemo-like), and for leukocyte like (leuko-like) genes inside the white adipose tissue of the perilipin-/- mice. In conclusion, microarrays have much to offer in comparative studies such as those in antiobesity, and also they are methodologies adequate for new astounding molecular discoveries [free full text of this article Online].
Castro-Chávez
Fernando
F
Departamento de Biología Molecular en Medicina, Hospital Civil de Belén, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, México. fdocc@yahoo.com
D000818:Animals; D002352:Carrier Proteins; D004041:Dietary Fats / Q000008:administration & dosage; D015870:Gene Expression; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors
D002352:Carrier Proteins; D004041:Dietary Fats; D009687:Nuclear Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins; D011506:Proteins; C051065:THRSP protein, human; C501477:Thrsp protein, mouse; D014157:Transcription Factors; D013230:Stearoyl-CoA Desaturase