Proteomics and physiology of erythritol-producing strains.
In-depth knowledge bases on physiological properties of microbes are required to design a better microbial system at a gene level and to develop an industrially viable process in an optimized scheme. Proteomic analyses of industrially useful microorganisms are particularly important for achieving such objectives. In this review, industrial application of erythritol in food and pharmaceutical areas and proteomic techniques for erythritol-producing microbes were presented. Proteomic technologies for erythritol-producing strains such as Candida magnoliae contained protein or peptide sample preparation for two-dimensional electrophoresis and mass spectrometry, analysis of proteome with matrix assisted laser desorption-ionization/time-of-flight mass spectrometry, liquid chromatography/electrospray ionization/tandem mass spectrometry and similarity searching algorithms. The proteomic information was applied to predict the carbon metabolism of erythritol-synthesizing microorganisms.
Park
Yong-Cheol
YC
School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Korea.
Lee
Do-Yup
DY
Lee
Dae-Hee
DH
Kim
Hyo-Jin
HJ
Ryu
Yeon-Woo
YW
Seo
Jin-Ho
JH
D002175:Candida / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004896:Erythritol / Q000096:biosynthesis*; D016681:Genome, Fungal; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004896:Erythritol