Subcutaneous administration of collagen-polyvinylpyrrolidone down regulates IL-1beta, TNF-alpha, TGF-beta1, ELAM-1 and VCAM-1 expression in scleroderma skin lesions.
In this study the effect of collagen-polyvinylpyrrolidone (collagen-PVP) vs. triamcinolone acetonide (Triam) in scleroderma (SSc) skin lesions was evaluated. Ten SSc patients were treated weekly with subcutaneous injections of 0.2 mL Triam (8 mg/mL) or 0.2 mL collagen-PVP (1.66 mg collagen). Skin biopsies were obtained from lesions before and after treatment. Tissue sections were evaluated by histology and immunohistochemistry (ELAM-1, VCAM-1, IL-1beta, TNF-alpha, TGF-beta1 and PDGF). The corticoid-treated group showed abnormal tissue architecture while the biodrug-treatment restored cutaneous appendages and type I/III collagen proportion. Cytokine and adhesion molecule expression was almost inhibited with Triam, while collagen-PVP down-regulated it. Collagen-PVP improved the tissue architecture of SSc lesions and down-regulated some proinflammatory parameters, without the side effects induced by corticoids.
Furuzawa-Carballeda
J
J
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Immunology and Rheumatology, Mexico. furuzawa@servidor.unam.mx
Krötzsch
E
E
Barile-Fabris
L
L
Alcalá
M
M
Espinosa-Morales
R
R
D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004311:Double-Blind Method; D015536:Down-Regulation; D019040:E-Selectin / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007279:Injections, Subcutaneous; D008875:Middle Aged; D011205:Povidone / Q000008:administration & dosage* / Q000494:pharmacology; D012595:Scleroderma, Systemic / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014222:Triamcinolone Acetonide / Q000008:administration & dosage* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015815:Cell Adhesion Molecules; D016207:Cytokines; D019040:E-Selectin; D005938:Glucocorticoids; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D014222:Triamcinolone Acetonide; D011205:Povidone