Rapid separation of microorganisms by quartz microchip capillary electrophoresis.
We developed and optimized a system coupling microchip capillary electrophoresis (MCE) and laser-induced fluorescence (LIF) detection for the analysis of microorganisms. The MCE-LIF system successfully separated pure cultures of lactic acid bacteria and Saccharomyces cerevisiae within 200 s. The results indicate that the MCE system can be conveniently used for the rapid and highly sensitive detection of microorganisms. Thus, MCE can provide a cheap and simple method for the on-line detection of microbial contamination.
Shintani
Tomoyoshi
T
Textile Industrial Institute of Ehime Prefecture, 2-5-48 Higashimura-minami Imabari, Ehime 799-1507, Japan. shintani@iri.pref.ehime.jp
Torimura
Masaki
M
Sato
Hiroaki
H
Tao
Hiroaki
H
Manabe
Takashi
T
D001419:Bacteria / Q000302:isolation & purification*; D046209:Electrophoresis, Microchip; D048668:Lactobacillus delbrueckii / Q000302:isolation & purification; D046208:Microchip Analytical Procedures; D011791:Quartz; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification; D048249:Streptococcus thermophilus / Q000302:isolation & purification
D011791:Quartz