title: New lignan metabolites in rat urine.
abstract: Ten potential lignan metabolites were quantified in rat urine extracts using liquid chromatography-tandem mass spectrometry. The rats were orally administered with the plant lignans 7-hydroxymatairesinol, matairesinol, lariciresinol or secoisolariciresinol, or with the mammalian lignan enterolactone. The samples were enzymatically hydrolysed and solid-phase extracted before analysis. Of the analysed compounds, only trace amounts of 7-oxoenterolactone could be detected in the urine extracts before administration, but after administration of any of the lignans, the excretion of 7-oxoenterolactone increased and monodemethylated matairesinol and 4,4'-dihydroxyenterolactone could be detected. In addition, other novel lignan metabolites were detected, i.e., 7-oxomatairesinol, alpha-conidendrin, and alpha- and beta-conidendric acid.
authors.affiliation: Department of Organic Chemistry, Abo Akademi University, Biskopsgatan 8, FIN-20500 Turku/Abo, Finland. ansmeds@abo.fi
authors.forename: Annika I
authors.initials: AI
authors.lastname: Smeds
authors.forename: Niina M
authors.initials: NM
authors.lastname: Saarinen
authors.forename: Patrik C
authors.initials: PC
authors.lastname: Eklund
authors.forename: Rainer E
authors.initials: RE
authors.lastname: Sjöholm
authors.forename: Sari I
authors.initials: SI
authors.lastname: Mäkelä
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017705:Lignans / Q000378:metabolism* / Q000652:urine*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017705:Lignans; C419306:hydroxymatairesinol