Herbicide resistance in transgenic plants with mammalian P450 monooxygenase genes.
Transgenic potato and rice plants were generated by the introduction of human P450 species, CYP1A1, CYP2B6, CYP2C9 and CYP2C19, which metabolized a number of herbicides, insecticides and industrial chemicals. The transgenic potato plant T1977 co-expressing CYP1A1, CYP2B6 and CYP2C19 genes showed remarkable cross-resistance to several herbicides with different structures and modes of action due to metabolism of these herbicides by the P450 species expressed. The transgenic rice plant 2C9-57R2 expressing CYP2C9 gene showed resistance to sulfonylureas, and the transgenic rice plant 2C19-12R1 expressing CYP2C19 gene showed cross-resistance to certain herbicides with different structures and modes of action. These transgenic plants appear to be useful for herbicide resistance as well as phytoremediation of environmental contaminants.
Inui
Hideyuki
H
Research Center for Environmental Genomics, Kobe University, Kobe, 657-8501, Japan.
Ohkawa
Hideo
H
D000818:Animals; D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D004351:Drug Resistance / Q000235:genetics; D006540:Herbicides / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007306:Insecticides / Q000378:metabolism; D008322:Mammals; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000201:enzymology
D006540:Herbicides; D007306:Insecticides; D003577:Cytochrome P-450 Enzyme System