Lactone synthesis activity in a site-directed mutant of an extradiol catechol dioxygenase enzyme.
A H115Y site-directed mutant of extradiol catechol dioxygenase MhpB catalyses an intramolecular lactonisation reaction upon its natural substrate.
Mendel
Sharon
S
University of Warwick, Coventry CV4 7AL, UK.
Arndt
Andrew
A
Bugg
Timothy D H
TD
D019943:Amino Acid Substitution; D002384:Catalysis; D049308:Dioxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007783:Lactones / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D007783:Lactones; D010105:Oxygenases; D049308:Dioxygenases; C083582:3-carboxyethylcatechol 2,3-dioxygenase; C439587:extradiol dioxygenase