title: Quantitative neuropathology of the entorhinal cortex region in patients with hippocampal sclerosis and temporal lobe epilepsy.
abstract: OBJECTIVE
  Clinical, radiologic, and experimental evidence indicates that the entorhinal cortex (EC) region may be linked to the pathophysiology of hippocampal sclerosis (HS) in patients with temporal lobe epilepsy. Few neuropathologic studies of this region have been undertaken in patients with HS undergoing surgery, some suggesting preferential loss of layer III neurones.
  
  
  METHODS
  We carried out a quantitative analysis in 26 patients with HS, nine patients with lesional temporal lobe epilepsy (LTLE), and eight postmortem controls. We measured neuronal densities in EC by using a three-dimensional cell-counting technique on NeuN immunostained and Nissl-stained sections. We also quantified the density of calretinin-positive interneurones in this region and the density of neurones in adjacent subiculum and CA1 subfields. We also assessed the patterns of gliosis in the EC in the patient groups and the presence of any neocortical neurone loss.
  
  
  RESULTS
  No significant difference was found in the mean neuronal densities in the EC region between HS and LTLE groups or postmortem controls. Laminar gliosis in midcortical layers was seen in a proportion of HS cases but also in the LTLE group. No significant difference was seen in the density of calretinin interneurones and no correlation between the presence of neocortical neuronal loss and EC neuronal densities.
  
  
  CONCLUSIONS
  A stereotypical pattern of neuronal loss and gliosis in the EC region in patients with HS is not confirmed that distinguishes this pathologic process from that in patients with lesional TLE.
authors.affiliation: Division of Neuropathology and Department of Clinical and Experimental Epilepsy, National Hospital for Neurology and Neurosurgery and Institute of Neurology, London, England.
authors.forename: Stephen
authors.initials: S
authors.lastname: Dawodu
authors.forename: Maria
authors.initials: M
authors.lastname: Thom
mesh_terms: D000818:Animals; D038481:Anterior Temporal Lobectomy; D001927:Brain Diseases / Q000473:pathology*; D002452:Cell Count; D004195:Disease Models, Animal; D018728:Entorhinal Cortex / Q000473:pathology*; D004833:Epilepsy, Temporal Lobe / Q000473:pathology* / Q000601:surgery; D005911:Gliosis / Q000473:pathology; D006624:Hippocampus / Q000473:pathology* / Q000601:surgery; D006801:Humans; D009474:Neurons / Q000473:pathology; D012598:Sclerosis / Q000473:pathology; D013702:Temporal Lobe / Q000473:pathology / Q000601:surgery