title: Interaction of U-box E3 ligase SNEV with PSMB4, the beta7 subunit of the 20 S proteasome.
abstract: Recognition of specific substrates for degradation by the ubiquitin-proteasome pathway is ensured by a cascade of ubiquitin transferases E1, E2 and E3. The mechanism by which the target proteins are transported to the proteasome is not clear, but two yeast E3s and one mammalian E3 ligase seem to be involved in the delivery of targets to the proteasome, by escorting them and by binding to the 19 S regulatory particle of the proteasome. In the present study, we show that SNEV (senescence evasion factor), a protein with in vitro E3 ligase activity, which is also involved in DNA repair and splicing, associates with the proteasome by directly binding to the beta7 subunit of the 20 S proteasome. Upon inhibition of proteasome activity, SNEV does not accumulate within the cells although its co-localization with the proteasome increases significantly. Since immunofluorescence microscopy also shows increased co-localization of SNEV with ubiquitin after proteasome inhibition, without SNEV being ubiquitinated by itself, we suggest that SNEV shows E3 ligase activity not only in vitro but also in vivo and escorts its substrate to the proteasome. Since the yeast homologue of SNEV, Prp19, also interacts with the yeast beta7 subunit of the proteasome, this mechanism seems to be conserved during evolution. Therefore these results support the hypothesis that E3 ligases might generally be involved in substrate transport to the proteasome. Additionally, our results provide the first evidence for a physical link between components of the ubiquitin-proteasome system and the spliceosome.
authors.affiliation: Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Marlies
authors.initials: M
authors.lastname: Löscher
authors.forename: Klaus
authors.initials: K
authors.lastname: Fortschegger
authors.forename: Gustav
authors.initials: G
authors.lastname: Ritter
authors.forename: Martina
authors.initials: M
authors.lastname: Wostry
authors.forename: Regina
authors.initials: R
authors.lastname: Voglauer
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Schmid
authors.forename: Steven
authors.initials: S
authors.lastname: Watters
authors.forename: A Jennifer
authors.initials: AJ
authors.lastname: Rivett
authors.forename: Paul
authors.initials: P
authors.lastname: Ajuh
authors.forename: Angus I
authors.initials: AI
authors.lastname: Lamond
authors.forename: Hermann
authors.initials: H
authors.lastname: Katinger
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D017173:Caenorhabditis elegans; D002352:Carrier Proteins / Q000737:chemistry; D002460:Cell Line; D017124:Conserved Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000502:physiology; D045643:DNA Repair Enzymes; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D000072260:RNA Splicing Factors; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry* / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D044767:Ubiquitin-Protein Ligases; D003546:Cysteine Endopeptidases; C108898:PSMB4 protein, human; D046988:Proteasome Endopeptidase Complex; D045643:DNA Repair Enzymes; C417041:PRPF19 protein, human