title: Leukemia inhibitory factor induces multi-lineage differentiation of adult stem-like cells in kidney via kidney-specific cadherin 16.
abstract: Side population (SP) is reported to be a stem cell-rich population. In the presence of leukemia inhibitory factor (LIF), cultured kidney SP cells differentiated into multi-lineage in collagen gel but not in synthesized polymer that has no cell adhesion factor. In cultured kidney SP cells, gene expression of kidney-specific cadherin 16 was specifically upregulated in collagen gel but not in synthesized polymer. Moreover, decreasing cadherin 16 expression using siRNA abolished LIF-induced multi-lineage differentiation of kidney SP in collagen gel. These results indicated that LIF induced multi-lineage differentiation of adult stem-like cells in kidney via cadherin 16.
authors.affiliation: Department of Clinical Renal Regeneration, Graduate School of Medicine, University of Tokyo, Japan. hishikawa-tky@umin.ac.jp
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hishikawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Marumo
authors.forename: Shigeki
authors.initials: S
authors.lastname: Miura
authors.forename: Asato
authors.initials: A
authors.lastname: Nakanishi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Matsuzaki
authors.forename: Katsunori
authors.initials: K
authors.lastname: Shibata
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kohike
authors.forename: Takuya
authors.initials: T
authors.lastname: Komori
authors.forename: Matsuhiko
authors.initials: M
authors.lastname: Hayashi
authors.forename: Toshio
authors.initials: T
authors.lastname: Nakaki
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakauchi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Toshiro
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019070:Cell Lineage / Q000187:drug effects / Q000502:physiology; D015850:Interleukin-6 / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000145:classification / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D015820:Cadherins; C491946:Cdh16 protein, mouse; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse