[Content determination of psoralen in root of Ficus hitra by HPLC method].
OBJECTIVE
To investigate the contents of psoralen in Ficus hitra.


METHODS
High Performance Liquid Chromatography was performed on Diamonsil C18. The chromatographic conditions were as follows: methanol-water (60:40) as mobile phase, flow rate being 1 ml/min and detecting wavelength at 245nm.


RESULTS
A good linearity of psoralen was shown in range of 0. 356 - 2. 848 mg/ml (r = 0.9999), the average recovery is 103.99%, RSD = 3.07% (n = 5). The method has good reproducibility, RSD = 1.59%


CONCLUSIONS
This method can supply evidence for the quality evaluation of Ficus hitra.
Liu
Chunling
C
Guangzhou University of Traditional Chinese Medicine, Guangzhou.
Xu
Honghua
H
Xu
Xiaofeng
X
Luo
Qisan
Q
Peng
Qianglin
Q
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030681:Ficus / Q000737:chemistry*; D005363:Ficusin / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D005363:Ficusin