RNAi as a tool to study cell biology: building the genome-phenome bridge.
In the few short years since its discovery, RNA interference (RNAi) has revolutionized the functional analysis of genomes: both technical and conceptual approaches to the investigation of gene function are being transformed as a result of this new technology. Genome-scale RNAi analyses have already been performed in the model organisms Caenorhabditis elegans (in vivo) and Drosophila melanogaster (in cell lines), ushering in a new era of RNAi-based approaches to probing the inner workings of the cell. The transformation of complex phenotypic data into mineable 'digitized' formats is fostering the emergence of a new area of bioinformatics related to the phenome.
Gunsalus
Kristin C
KC
Center for Comparative Functional Genomics, Department of Biology, New York University, 1009 Silver Center, 100 Washington Sq E, New York, New York 10003, USA.
Piano
Fabio
F
D000818:Animals; D001695:Biology / Q000379:methods*; D017173:Caenorhabditis elegans; D003585:Cell Biology; D004331:Drosophila melanogaster; D005821:Genetic Techniques; D016678:Genome; D006801:Humans; D010641:Phenotype; D034622:RNA Interference; D012984:Software