title: pH-dependent quenching of the fluorescence of tryptophan residues in class A beta-lactamase from E. coli (TEM-1).
abstract: We performed an investigation of the pH-dependent quenching of the fluorescence of tryptophan residues of TEM-1 beta-lactamase from E. coli by uncharged and charged quenchers. pH-dependent Stern-Volmer constants (Ksv/pH) of tryptophan residues allowed us to determine subtle but discrete structurally and functionally important processes.
authors.affiliation: Institute of Organic Chemistry Bulgarian Academy of Science, Sofia 1113, Bulgaria. christov@sg.uji.es
authors.forename: Christo
authors.initials: C
authors.lastname: Christov
authors.forename: Dimitar
authors.initials: D
authors.lastname: Ianev
authors.forename: Alexandra
authors.initials: A
authors.lastname: Shosheva
authors.forename: Boris
authors.initials: B
authors.lastname: Atanasov
mesh_terms: D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013050:Spectrometry, Fluorescence / Q000379:methods; D014364:Tryptophan; D001618:beta-Lactamases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D029968:Escherichia coli Proteins; D014364:Tryptophan; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1