[Protection of vascular endothelial cells from TNF-alpha induced injury by quercetin].
OBJECTIVE
To investigate the effects of quercetin (Que) on the TNF-alpha injured human umbilical vein endothelial cell line (ECV-304).


METHODS
The experiments were performed in culture of TNF-alpha-induced ECV-304 in vitro. Cell viability was assessed with MTT assay. Lipid peroxidation of ECV-304 was monitored as malonaldehyde (MDA) with a fluorometric assay. NO and SOD of ECV-304 were monitored with colorimetry. The expression of NF-kappaB was detected with immunocytochemistry.


RESULTS
TNF-alpha inhibited the ECV-304 prolifteration. Preincubation of ECV-304 with Que for 24h before TNF-alpha exposure increased the cell viability. Que significantly decreased the level of NO and MDA and the expression of NF-kappaB, and increased the activity of SOD in TNF-alpha-induced ECV-304.


CONCLUSIONS
These results demonstrate that Que can produce the protective action on TNF-alpha-induced cultured ECV-304 and its mechanism of action may be related to the decrease of NO, antioxidant effect of lipids. However, antioxidant effect might be exerted through NF-kappaB activation pathway.
Lin
Rong
R
Medical School of Xi' an Jiaotong University, Xi'an.
Liu
Juntian
J
Gan
Weijie
W
D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis*; D009569:Nitric Oxide / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008315:Malondialdehyde; D011794:Quercetin; D013482:Superoxide Dismutase