Mouse testican-2. Expression, glycosylation, and effects on neurite outgrowth.
Mouse testican-2 was cloned, sequenced, and shown to be a proteoglycan with a multidomain structure closely similar to that of the human ortholog, previously described as a calcium binding extracellular matrix molecule of the BM-40/SPARC/osteonectin family (Vannahme, C., Schübel, S., Herud, M., Gösling, S., Hülsmann, H., Paulsson, M., Hartmann, U., and Maurer, P. (1999). J. Neurochem. 73, 12-20). Recombinant mouse testican-2 was used to prepare specific antibodies that allowed the detection of testican-2 in various brain structures but also in lung, testis, and in several endocrine glands. Although the testican-2 expressed in EBNA-293 cells carried both heparan sulfate and chondroitin/dermatan sulfate glycosaminoglycan chains, the tissue form always contained only heparan sulfate. Both tissue-derived and recombinant testican-2 carried N-linked glycans. Tissue-derived forms of testican-2 were detected as proteoglycans of varying size, whereas a portion of the molecules produced by EBNA-293 cells were core proteins, lacking glycosaminoglycans. Both the proteoglycan and core protein forms of testican-2 inhibited neurite extension from cultured primary cerebellar neurons and may play regulatory roles in the development of the central nervous system.
Schnepp
Anke
A
Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany.
Komp Lindgren
Patricia
P
Hülsmann
Hanni
H
Kröger
Stephan
S
Paulsson
Mats
M
Hartmann
Ursula
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019084:Fluorescent Antibody Technique, Indirect; D006031:Glycosylation; D051379:Mice; D008969:Molecular Sequence Data; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D011509:Proteoglycans / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology / Q000502:physiology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology
D011509:Proteoglycans; D011994:Recombinant Proteins; C504404:SPOCK2 protein, human; C504370:Spock1 protein, mouse; C504405:Spock2 protein, mouse