Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU.
Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.
Sato
Hiromi
H
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226, USA.
Feix
Jimmy B
JB
Hillard
Cecilia J
CJ
Frank
Dara W
DW
D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism; D007700:Kinetics; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013379:Substrate Specificity; D013816:Thermodynamics
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D010743:Phospholipids; C111749:pseudomonas exoprotein A protein, Pseudomonas aeruginosa; D010740:Phospholipases