Role of oxidative phosphorylation in histatin 5-induced cell death in Saccharomyces cerevisiae.
The susceptibility of Saccharomyces cerevisiae to the anti-microbial peptide, histatin 5, was tested after pre-growth in fermentable and non-fermentable carbon sources and in the absence or presence of the uncoupler of oxidative phosphorylation, carbonyl cyanide m-chlorophenylhydrazone (CCCP). S. cerevisiae was more resistant to histatin 5 when grown on a fermentable carbon source compared to growth on a non-fermentable carbon source, indicating an important role for oxidative phosphorylation in histatin 5-induced cell death. Oxidative phosphorylation is a pre-requisite for histatin 5-induced cell death in Candida albicans but this is not the case in S. cerevisiae. Incubation of CCCP-treated S. cerevisiae cells with histatin 5 still resulted in cell death. These results suggest that histatin 5-induced cell death in S. cerevisiae differs from that in C. albicans.
De Smet
Kris
K
Unit of Fundamental and Applied Molecular Biology, Department for Molecular Biomedical Research, Ghent University and VIB, Technologiepark 927, B-9052 Ghent, Belgium.
Reekmans
Rieka
R
Contreras
Roland
R
D017209:Apoptosis / Q000187:drug effects*; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004347:Drug Interactions; D005947:Glucose / Q000378:metabolism; D054808:Histatins; D010084:Oxidation-Reduction / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D012471:Salivary Proteins and Peptides / Q000494:pharmacology*; D013045:Species Specificity
C068075:HTN3 protein, human; D054808:Histatins; D012471:Salivary Proteins and Peptides; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D005947:Glucose