Chloroperoxidase from Caldariomyces fumago is active in the presence of an ionic liquid as co-solvent.
Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylimidazolium methylsulfate. The enzyme activity in these ionic liquid co-solvent systems is retained for 24 h, but it falls to 3 h using non-ionic organic solvents such as t-BuOH or acetone.
Sanfilippo
Claudia
C
CNR Istituto Chimica Biomolecolare, Sezione CT, Via del Santuario 110, I-95028 Valverde CT, Italy. claudia.sanfilippo@icb.cnr.it
D'Antona
Nicola
N
Nicolosi
Giovanni
G
D001203:Ascomycota / Q000201:enzymology*; D002711:Chloride Peroxidase / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D002951:Citrates / Q000737:chemistry*; D004789:Enzyme Activation; D007477:Ions; D007700:Kinetics; D009281:Naphthalenes / Q000737:chemistry*; D012996:Solutions; D012997:Solvents / Q000737:chemistry*; D020122:tert-Butylhydroperoxide / Q000737:chemistry*
D002951:Citrates; D007477:Ions; D009281:Naphthalenes; D012996:Solutions; D012997:Solvents; C086079:1,2-dihydronaphthalene; D020122:tert-Butylhydroperoxide; D002711:Chloride Peroxidase