Induction of apoptosis in bone marrow cells is mediated via purinergic receptors.
ATP activity in mouse bone marrow cells was in vitro estimated by expression of phosphatidylserine on the outer membrane surface using FITC-labeled annexin. ATP induced apoptosis in bone marrow cells. Purinergic receptor antagonists PPADS and suramin modulated the apoptotic effect of ATP on hemopoietic cells. Acute and subacute administration of doxorubicin, an inductor of oxidative burst, decreased cell sensitivity to ATP and abolished its apoptotic effect.
Uspenskaya
Yu A
YA
Department for Animal Anatomy and Physiology, Krasnoyarsk State Agrarian University.
Mikhutkina
S V
SV
Taksanova
E I
EI
Popova
N N
NN
Olovyannikova
R Ya
RY
Salmina
A B
AB
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology; D008297:Male; D051379:Mice; D009336:Necrosis / Q000503:physiopathology; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D011983:Receptors, Purinergic / Q000502:physiology*; D013498:Suramin / Q000494:pharmacology
D011983:Receptors, Purinergic; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; D011732:Pyridoxal Phosphate; D013498:Suramin; D004317:Doxorubicin; D000255:Adenosine Triphosphate