title: Technetium-99m-HMPAO labeled leukocyte single photon emission computerized tomography (SPECT) for assessing Crohn's disease extent and intestinal infiltration.
abstract: OBJECTIVE
  Scintigraphy using radiolabeled leukocytes is a useful technique for assessing intestinal infiltration in Crohn's disease (CD). However, limits of planar images include overlapping activity in other organs and low specificity. To investigate the usefulness of (99m)Tc-HMPAO (hexametyl propylene amine oxime) labeled leukocyte single photon emission computerized tomography (SPECT) for assessing CD lesions, in comparison with planar images.
  
  
  METHODS
  Twenty-two inflammatory bowel disease patients (19 CD; 2 ulcerative colitis, UC; 1 ileal pouch) assessed by conventional endoscopy or radiology were enrolled. Leukocytes were labeled with (99m)Tc-HMPAO. SPECT images were acquired at 2 h and planar images at 30 min and 2 h. Bowel uptake was quantitated in nine regions (score 0-3).
  
  
  RESULTS
  Both SPECT and planar images detected a negative scintigraphy (score 0) in the UC patient with no pouchitis and a positive scintigraphy (score 1-3) in the 21 patients showing active inflammation by conventional techniques. SPECT showed a higher global score than planar images (0.71 +/- 0.09 vs 0.30 +/- 0.05; p < 0.001), and in particular in the right iliac fossa (p= 0.003), right and left flank (p < 0.001; p= 0.02), hypogastrium (p= 0.002), and mesogastrium (p < 0.001). SPECT provided a better visualization and a higher uptake than planar images in patients with ileal and ileocolonic CD (6.45 +/- 0.82 vs 2.8 +/- 0.55, p < 0.001; 5.5 +/- 1.6 vs 2.6 +/- 0.7, p= 0.03), and with perianal CD (6.6 +/- 1.6 vs 3.4 +/- 1.2; p= 0.03).
  
  
  CONCLUSIONS
  (99m)Tc-HMPAO labeled leukocyte SPECT provides a more detailed visualization of CD lesions than planar images. This technique may better discriminate between intestinal and bone marrow uptake, thus being useful for assessing CD lesions within the pelvis, including perianal disease.
authors.affiliation: Dipartimento di Medicina Interna, Cattedra di Gastroenterologia, Università di Roma Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. biancone@med.uniroma2.it
authors.forename: L
authors.initials: L
authors.lastname: Biancone
authors.forename: O
authors.initials: O
authors.lastname: Schillaci
authors.forename: F
authors.initials: F
authors.lastname: Capoccetti
authors.forename: R M
authors.initials: RM
authors.lastname: Bozzi
authors.forename: D
authors.initials: D
authors.lastname: Fina
authors.forename: C
authors.initials: C
authors.lastname: Petruzziello
authors.forename: A
authors.initials: A
authors.lastname: Geremia
authors.forename: G
authors.initials: G
authors.lastname: Simonetti
authors.forename: F
authors.initials: F
authors.lastname: Pallone
mesh_terms: D003093:Colitis, Ulcerative / Q000000981:diagnostic imaging; D003424:Crohn Disease / Q000000981:diagnostic imaging* / Q000473:pathology; D005260:Female; D006801:Humans; D007422:Intestines / Q000000981:diagnostic imaging* / Q000473:pathology; D007553:Isotope Labeling; D007962:Leukocytes; D008297:Male; D008875:Middle Aged; D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime