title: Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting.
abstract: Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.
authors.affiliation: Department of Experimental, Environmental Medicine and Medical Biotechnologies, University of Milano-Bicocca, Monza, Italy. fulvio.magni@unimib.it
authors.forename: Fulvio
authors.initials: F
authors.lastname: Magni
authors.forename: Cecilia
authors.initials: C
authors.lastname: Sarto
authors.forename: Cristina
authors.initials: C
authors.lastname: Valsecchi
authors.forename: Stefano
authors.initials: S
authors.lastname: Casellato
authors.forename: Stefano Ferrero
authors.initials: SF
authors.lastname: Bogetto
authors.forename: Silvano
authors.initials: S
authors.lastname: Bosari
authors.forename: Andrea
authors.initials: A
authors.lastname: Di Fonzo
authors.forename: Roberto A
authors.initials: RA
authors.lastname: Perego
authors.forename: Matteo
authors.initials: M
authors.lastname: Corizzato
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Doro
authors.forename: Carmen
authors.initials: C
authors.lastname: Galbusera
authors.forename: Francesco
authors.initials: F
authors.lastname: Rocco
authors.forename: Paolo
authors.initials: P
authors.lastname: Mocarelli
authors.forename: Marzia
authors.initials: M
authors.lastname: Galli Kienle
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007672:Kidney Cortex / Q000737:chemistry*; D010449:Peptide Mapping; D020033:Protein Isoforms / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D012016:Reference Values; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020033:Protein Isoforms; D020543:Proteome