title: Cone function studied with flicker electroretinogram during progressive retinal degeneration in RCS rats.
abstract: The Royal College of Surgeons (RCS) rat has a primary defect in retinal pigment epithelial cells that leads to the progressive loss of photoreceptors and central visual responsiveness. While most rods are lost by 90 days of age (P90), cones degenerate more slowly, and can be detected anatomically up to 2 years of age, despite massive neuronal death and retinal remodelling. To examine how this progressive degenerative process impacts on cone function, we recorded the electroretingram to white light flashes (1.37 log cd s m(-2)) presented at frequencies ranging from 3 to 50 Hz, under light adapted conditions (29.8 cd m(-2)). Pigmented dystrophic and congenic non-dystrophic RCS rats aged from 18 to 300 days were studied. In all responsive animals at all ages, maximal amplitudes were obtained at 3 Hz. In both non-dystrophic and dystrophic rats, there was an increase from P18 to P21 in response amplitude and critical fusion frequency. After P21, these two parameters declined progressively with age in dystrophic rats. Other changes included prolongation in latency, which was first detected prior to the initiation of amplitude reduction. While phase shifts were also detected in dystrophic RCS rats, they appeared at later degenerative stages. The latest age at which responses could be elicited in dystrophic rats was at P200, with positive waves being replaced by negative deflections. The effect of increments in the intensity of background illumination was tested at P50 in both groups. This caused a diminution in flicker response amplitude and critical fusion frequencies in non-dystrophics, while in dystrophic animals, response amplitudes were reduced only at low frequencies and critical fusion frequencies were unaltered. In conclusion, although dystrophic RCS rats undergo a progressive decline in cone function with age, the flicker responsiveness at P21 is comparable to that of non-dystrophic congenic rats, suggesting normal developmental maturation of the cone system in this animal model of retinal degeneration. Flicker responses can be recorded up to P200, at which point the retina has undergone severe regressive and reactive changes in its connectivity patterns. The fact that responses at this age consist of solely negative deflections might be a reflection of the highly pathological state of the retina.
authors.affiliation: Moran Eye Center, Ophthalmology and Visual Sciences, University of Utah, 75 North Medical Drive, Salt Lake City, UT 84132, USA.
authors.forename: I
authors.initials: I
authors.lastname: Pinilla
authors.forename: R D
authors.initials: RD
authors.lastname: Lund
authors.forename: Y
authors.initials: Y
authors.lastname: Sauvé
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D004596:Electroretinography / Q000379:methods; D004847:Epithelial Cells / Q000502:physiology; D005425:Flicker Fusion / Q000502:physiology; D010775:Photic Stimulation / Q000379:methods; D010857:Pigment Epithelium of Eye / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D011930:Reaction Time / Q000502:physiology; D017949:Retinal Cone Photoreceptor Cells / Q000503:physiopathology*; D012162:Retinal Degeneration / Q000503:physiopathology*; D012174:Retinitis Pigmentosa / Q000503:physiopathology