title: Subcutaneous administration of collagen-polyvinylpyrrolidone down regulates IL-1beta, TNF-alpha, TGF-beta1, ELAM-1 and VCAM-1 expression in scleroderma skin lesions.
abstract: In this study the effect of collagen-polyvinylpyrrolidone (collagen-PVP) vs. triamcinolone acetonide (Triam) in scleroderma (SSc) skin lesions was evaluated. Ten SSc patients were treated weekly with subcutaneous injections of 0.2 mL Triam (8 mg/mL) or 0.2 mL collagen-PVP (1.66 mg collagen). Skin biopsies were obtained from lesions before and after treatment. Tissue sections were evaluated by histology and immunohistochemistry (ELAM-1, VCAM-1, IL-1beta, TNF-alpha, TGF-beta1 and PDGF). The corticoid-treated group showed abnormal tissue architecture while the biodrug-treatment restored cutaneous appendages and type I/III collagen proportion. Cytokine and adhesion molecule expression was almost inhibited with Triam, while collagen-PVP down-regulated it. Collagen-PVP improved the tissue architecture of SSc lesions and down-regulated some proinflammatory parameters, without the side effects induced by corticoids.
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Immunology and Rheumatology, Mexico. furuzawa@servidor.unam.mx
authors.forename: J
authors.initials: J
authors.lastname: Furuzawa-Carballeda
authors.forename: E
authors.initials: E
authors.lastname: Krötzsch
authors.forename: L
authors.initials: L
authors.lastname: Barile-Fabris
authors.forename: M
authors.initials: M
authors.lastname: Alcalá
authors.forename: R
authors.initials: R
authors.lastname: Espinosa-Morales
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004311:Double-Blind Method; D015536:Down-Regulation; D019040:E-Selectin / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007279:Injections, Subcutaneous; D008875:Middle Aged; D011205:Povidone / Q000008:administration & dosage* / Q000494:pharmacology; D012595:Scleroderma, Systemic / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014222:Triamcinolone Acetonide / Q000008:administration & dosage* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D019040:E-Selectin; D005938:Glucocorticoids; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D014222:Triamcinolone Acetonide; D011205:Povidone