Rapid and selective micellar electrokinetic chromatography for simultaneous determination of amikacin, kanamycin A, and tobramycin with UV detection and application in drug formulations.
A simple and selective micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous determination of amikacin, tobramycin, and kanamycin A, performed in Tris buffer (180 mM; pH 9.1) with 300 mM sodium pentanesulfonate (SPS) as an anionic surfactant. Under this condition, good separation with high efficiency and the required short analysis time is achieved. The linear ranges of the method for the determination of amikacin, tobramycin, and kanamycin A were 0.1-0.5 mg / mL, 0.4-2.0 mg / mL, and 0.4-2.0 mg / mL, respectively; the detection limits (signal-to-noise ratio = 3; injection, 0.5 psi 5 s) were 0.08, 0.2, and 0.2 mg / mL, respectively. The small amount of sample required and the expeditiousness of the procedure allow content uniformity to be determined in individual commercial products.
Yeh
Hsin-Hua
HH
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University.
Lin
Shun-Jin
SJ
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University.
Ko
Ju-Yun
JY
School of Medicine, Kaohsiung Medical University.
Chou
Chia-An
CA
School of Medicine, Kaohsiung Medical University.
Chen
Su-Hwei
SH
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University.|Department of Pharmacy, Kaohsiung Medical University, Chung-Ho Memorial Hospital, Kaohsiung, Taiwan.
D017738:Alkanesulfonic Acids / Q000737:chemistry; D000583:Amikacin / Q000032:analysis / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007612:Kanamycin / Q000032:analysis / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014031:Tobramycin / Q000032:analysis / Q000302:isolation & purification*; D014325:Tromethamine
D017738:Alkanesulfonic Acids; D004364:Pharmaceutical Preparations; D014325:Tromethamine; C086946:1-pentanesulfonic acid; D007612:Kanamycin; D000583:Amikacin; D014031:Tobramycin