Effects of bemiparin on airway responses to antigen in sensitized Brown-Norway rats.
Heparins have demonstrated activity in asthma. The effects of bemiparin, a low molecular weight heparin, were examined on antigen-induced responses in sensitized Brown-Norway rats. Inhaled bemiparin (1 mg/ml) reduced the acute bronchospasm produced by aerosol antigen, prevented airway hyperresponsiveness to 5-hydroxytryptamine postantigen exposure, and reduced the eosinophil count (from 0.205+/-0.062 to 0.054+/-0.016 x 10(6) cells/ml in antigen and antigen+bemiparin groups, respectively; P<0.05), eosinophil peroxidase activity, and proteins in the bronchoalveolar lavage fluid (BALF), as well as the transiently augmented mucin Muc5ac expression. Hyperresponsiveness to adenosine was not affected by bemiparin. In similar experiments, inhaled fondaparinux (1 mg/ml) did not affect the antigen-induced responses, while a low-anticoagulant low molecular weight heparin was effective. In conclusion, bemiparin showed beneficial effects in experimental asthma, probably unrelated to its anticoagulant activity, which extends the previous positive findings obtained with other heparins.
Suchankova
Jana
J
Department of Pharmacology, University of Valencia, Avenida Blasco Ibanez 15, E-46010 Valencia, Spain.
Mata
Manuel
M
Cortijo
Julio
J
Morcillo
Esteban J
EJ
D000818:Animals; D000941:Antigens / Q000633:toxicity*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology* / Q000503:physiopathology; D006495:Heparin, Low-Molecular-Weight / Q000627:therapeutic use*; D007114:Immunization; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN
D000941:Antigens; D006495:Heparin, Low-Molecular-Weight; C411345:bemiparin