An autoregulatory circuit for long-range self-organization in Dictyostelium cell populations.
Nutrient-deprived Dictyostelium amoebae aggregate to form a multicellular structure by chemotaxis, moving towards propagating waves of cyclic AMP that are relayed from cell to cell. Organizing centres are not formed by founder cells, but are dynamic entities consisting of cores of outwardly rotating spiral waves that self-organize in a homogeneous cell population. Spiral waves are ubiquitously observed in chemical reactions as well as in biological systems. Although feedback control of spiral waves in spatially extended chemical reactions has been demonstrated in recent years, the mechanism by which control is achieved in living systems is unknown. Here we show that mutants of the cyclic AMP/protein kinase A pathway show periodic signalling, but fail to organize coherent long-range wave territories, owing to the appearance of numerous spiral cores. A theoretical model suggests that autoregulation of cell excitability mediated by protein kinase A acts to optimize the number of signalling centres.
Sawai
Satoshi
S
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA. ssawai@molbio.princeton.edu
Thomason
Peter A
PA
Cox
Edward C
EC
D000818:Animals; D002633:Chemotaxis; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D025461:Feedback, Physiological; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D015398:Signal Transduction; D013997:Time Factors
D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases