The heminested RT-PCR for the study of rabies virus pathogenesis.
The aim of the present trial was to evaluate the heminested RT-PCR for the study of rabies virus distribution in mice inoculated experimentally. Inoculation was by the intramuscular route in 150 mice, using the dog street rabies virus. Groups of five animals were killed at different times. Fragments of different organs were collected and the material was tested by Fluorescent Antibody Test (FAT) and heminested RT-PCR (hn RT-PCR). Positive results were obtained beginning on the 10th day after inoculation in the brain, spinal cord, salivary gland, limbs, lungs, liver, spleen, urinary bladder, tongue and right kidney. Hn RT-PCR was shown to be more efficient for the study of rabies virus distribution in different tissues and organs.
Lima
K C
KC
School of Veterinary Medicine and Zootechny, Department of Veterinary Hygiene and Public Health, Botucatu, UNESP, Brazil.
Megid
J
J
Silva
A V
AV
Cortez
A
A
D000818:Animals; D005455:Fluorescent Antibody Technique; D051379:Mice; D011820:Rabies virus / Q000235:genetics / Q000472:pathogenicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*