The fabrication of protein chip based on surface plasmon resonance for detection of pathogens.
Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip.
Oh
Byung-Keun
BK
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
Lee
Woochang
W
Chun
Bum Suk
BS
Bae
Young Min
YM
Lee
Won Hong
WH
Choi
Jeong-Woo
JW
D001419:Bacteria / Q000276:immunology* / Q000302:isolation & purification*; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008422:Materials Testing; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000276:immunology*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D001672:Biocompatible Materials; C029152:G-substrate; D009419:Nerve Tissue Proteins