Ole e 2 and Ole e 10: new clinical aspects and genetic restrictions in olive pollen allergy.
BACKGROUND
The clinical characteristics in olive pollen allergy are dependent on the antigenic load, the allergens profile, and the genetic restrictions. Our objective was to determine specific response pattern in Ole e 2 and Ole e 10 sensitization at those levels.


METHODS
We studied 146 patients with seasonal rhinitis and/or asthma and positive prick test to Olea europaea pollen. IgE against Ole e 2 and Ole e 10 were detected by skin prick test and ELISA. HLA-DRB1 and HLA-DQB1 loci were typed by polymerase chain reaction sequence-specific primers method.


RESULTS
A total of 102 (69.9%) and 79 (54.0%) patients showed significant IgE antibody response against Ole e 2 and Ole e 10, respectively. There was a significant association between Ole e 2 (OR 2.2, P = 0.04) and Ole e 10 reactivities (OR 2.8, P = 0.007) with asthma. In addition, total and specific IgE antibody levels significantly correlated with asthma (P < 0.05). Patients who reacted to both allergens reached the highest asthma risk factor (OR 4.3, P = 0.002). Phenotypic frequency of DR7 (OR 5.4, Pc = 0.003) and DQ2 (OR 3.6, Pc = 0.02) were increased in positive Ole e 2 patients compared with control subjects. DR2(15) phenotypic frequency was significantly increased (OR 5.6, Pc = 0.02) in positive Ole e 10 patients compared with control subjects.


CONCLUSIONS
Our data suggest an association of Ole e 2 and Ole e 10 with bronchial asthma. Also, we found a genetic control of Ole e 2 and Ole e 10 IgE-specific responses that could be relevant to clinical disease in olive pollen allergy.
Quiralte
J
J
Unidad de Alergia, Complejo Hospitalario de Jaén, Jaén, Spain.
Llanes
E
E
Barral
P
P
Arias de Saavedra
J M
JM
Sáenz de San Pedro
B
B
Villalba
M
M
Florido
J F
JF
Rodríguez
R
R
Lahoz
C
C
Cárdaba
B
B
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant; D001249:Asthma / Q000209:etiology / Q000276:immunology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics; D015802:HLA-DR2 Antigen / Q000235:genetics; D015809:HLA-DR7 Antigen / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D006967:Hypersensitivity / Q000150:complications / Q000235:genetics / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D031658:Olea / Q000276:immunology*; D010641:Phenotype; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D012307:Risk Factors; D012882:Skin Tests
D000485:Allergens; D052179:Antigens, Plant; D006683:HLA-DQ Antigens; C053186:HLA-DQ2 antigen; D015802:HLA-DR2 Antigen; D015809:HLA-DR7 Antigen; C487628:Ole e 10 allergen, Olea europaea; C467887:Ole e 2 allergen, Olea europaea; D010940:Plant Proteins; D007073:Immunoglobulin E