Monitoring thiobarbituric acid-reactive substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system.
Oxidative stress is a pivotal factor in neuronal degeneration. A simple method to quantify oxidative damage in culture and in situ is therefore important for studies of neurodegeneration. We present herein modifications of the standard assay for thiobarbituric acid-reactive substances (TBARs) for analyses of both cell cultures and brain tissue homogenates. Since the TBAR assay measures end-point oxidative damage, it is useful to assess the overall impact of oxidative stress-inducing and neuroprotective agents; interpretation is not potentially confounded by the presence or absence of transient products of oxidative damage.
Dawn-Linsley
Maria
M
Department of Biological Sciences, Center for Cellular Neurobiology and Neurodegeneration Research, University of Massachusetts Lowell, Lowell, MA 01854, USA.
Ekinci
Fatma J
FJ
Ortiz
Daniela
D
Rogers
Eugene
E
Shea
Thomas B
TB
D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology; D018753:Diet, Protein-Restricted / Q000379:methods; D004622:Embryo, Mammalian; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008991:Monitoring, Physiologic / Q000379:methods*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000150:complications / Q000378:metabolism
D001057:Apolipoproteins E; D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide