Synergistic action of MLL, a TRX protein with template activating factor-I, a histone chaperone.
MLL is involved in the process of gene activity maintenance. It is shown that the amino-terminal region of MLL (MLLN) interacts with TAF-Ibeta/SET. In this study, using yeast two-hybrid assays, we have found that the acidic region of TAF-Ibeta is essential for its binding to MLLN. Pull-down assays using GST-MLLN demonstrated that TAF-Ibeta and histones interact with GST-MLLN. MLLN and TAF-Ibeta synergistically upregulated the transcription level of Hoxa9 and co-immunoprecipitated in chromatin containing the Hoxa9 promoter region. These results suggest that TAF-Ibeta plays an important role in MLL-mediated transcription and possibly chromatin maintenance.
Shimoyama
Tae
T
Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba 305-8505, Japan.
Kato
Kohsuke
K
Miyaji-Yamaguchi
Mary
M
Nagata
Kyosuke
K
D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism / Q000502:physiology*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D056488:Histone Chaperones; D011495:Histone-Lysine N-Methyltransferase; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D051788:Myeloid-Lymphoid Leukemia Protein; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011519:Proto-Oncogenes / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D018398:Homeodomain Proteins; C497422:KMT2A protein, human; C513473:SET protein, human; D014157:Transcription Factors; C097899:homeobox protein HOXA9; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase