Thermodynamics of a protein acylation: activation of Escherichia coli hemolysin toxin.
HlyC, hemolysin-activating lysine-acyltransferase, catalyses the acylation (from acyl-acyl carrier protein [ACP]) of Escherichia coli prohemolysin (proHlyA) on the epsilon-amino groups of specific lysine residues, 564 and 690 of the 1024 amino acid primary structure, to form hemolysin (HlyA). Isothermal titration calorimetry was used to measure the thermodynamic properties of the protein acylation of proHlyA-derived structures, altered by substantial deletions and separation of the acylation sites into two different peptides and site directed mutation analyses of acylation sites. Acylation of proHlyA-derived proteins catalyzed by HlyC was overall an exothermic reaction driven by a negative enthalpy. The reaction, whose kinetics are compatible to a ping-pong mechanism, is composed of two partial reactions. The first, the formation of an acyl-HlyC intermediate, was entropically driven, most likely by noncovalent complex formation between acyl-ACP and HlyC; enthalpy-driven acyl transfer followed, resulting in acyl-HlyC and ACPSH product formation. The second partial reaction was an energetically unfavorable acyl transfer from acyl-enzyme intermediate to the final acyl acceptor, a proHlyA derivative. Overall the acylation of proHlyA-derived proteins catalyzed by HlyC was driven by the energetics of the acyl enzyme intermediate reaction. Of the two acylation sites, intactness of the site equivalent to proHlyA K564 was more important for acylation reaction thermodynamic stability.
Worsham
Lesa M S
LM
Department of Biochemistry and Molecular Biology, James H. Quillen College of Medicine, Box 70, 581 East Tennessee State University, Johnson City, Tennessee 37614, USA.
Langston
Keisha G
KG
Ernst-Fonberg
M Lou
ML
D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049148:Aminoacylation; D002151:Calorimetry / Q000379:methods; D002384:Catalysis; D019277:Entropy; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013816:Thermodynamics
D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D010446:Peptide Fragments; D011498:Protein Precursors; D000217:Acyltransferases; C092237:HlyC protein, E coli