Monoxenic production of the entomopathogenic nematode Steinernema carpocapsae using culture media containing agave juice (aguamiel) from Mexican maguey-pulquero (Agave spp). Effects of the contents of nitrogen, carbohydrates and fat on infective juvenile production.
The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (aguamiel) from Mexican maguey-pulquero (Agave spp) as the main carbohydrate source. After 20 days of fermentation, a maximum viable IJ concentration of 249,000 IJ/ml and an initial nematode population multiplication factor of x620 were achieved when medium M4 was used (aguamiel concentration in this medium was 28% by vol.). M4 medium contained (w/v): 0.3% total nitrogen, 3.2% total carbohydrates and 3.0% total fat. According to the results obtained, total carbohydrates concentration appeared to be of great importance in obtaining high IJ concentrations.
Islas-López
Marco-Antonio
MA
Centro de Investigaciones en Ciencia y Tecnología de los Alimentos, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Avenida Universidad km 1, Rancho Universitario, CP 43600 Tulancingo, Hidalgo, Mexico.
Sanjuan-Galindo
René
R
Rodríguez-Hernández
Adriana-Inés
AI
Chavarría-Hernández
Norberto
N
D027882:Agave / Q000737:chemistry*; D000818:Animals; D050260:Carbohydrate Metabolism; D003470:Culture Media / Q000737:chemistry*; D005223:Fats / Q000378:metabolism*; D008800:Mexico; D009584:Nitrogen / Q000378:metabolism*; D010572:Pest Control, Biological; D017168:Rhabditida / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D013997:Time Factors; D020593:Xenorhabdus
D003470:Culture Media; D005223:Fats; D009584:Nitrogen