title: The nuclear 16-kD protein methylation increases in the early period of liver regeneration in a hepatectomized rat.
abstract: Methylation events play a critical role in various cellular processes including regulation of gene transcription and proliferation. We observed that methyltransferase activity underwent time-dependent changes in the cytosol of the rat hepatocytes upon partial hepatectomy. However,any change in the methylation of nuclear proteins is not clear during hepatocyte proliferation. The nuclear fraction possesses basal level of methyltransferase to catalyze methylation of several proteins ranging from 7 to 70 kD prior to any hepatecmony. The specific p16 (16 kD)band was transiently and heavily methylated post 1 day hepatectomy, and then became non-detectable, but not in the control liver. Methylation of p16 band was completely inhibited by exogenously added histones, particularly 2AS, 1,2A and 2B subtypes. The methylated p16 protein remains stable in either acid or alkali- induced demethylation conditions, indicating that methylation is not likely to occur on isoaspartyl or C-terminal cysteinyl residues. Exogenous addition of non-hydrolyzable GTP caused a dose-dependent suppression of a p16 methylation suggesting that G-proteins might play a role as an endogenous methylation inhibitor in vivo. Taken together, we have identified the proliferation event associated-methylation of the nuclear p16 protein in the hepatocytes undergoing liver regeneration.
authors.affiliation: Department of Biochemistry, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Kyounghwa
authors.initials: K
authors.lastname: Lee
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Lee
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Kim
authors.forename: Meyoung Kon
authors.initials: MK
authors.lastname: Kim
authors.forename: Jong Seol
authors.initials: JS
authors.lastname: Han
authors.forename: Yoon Sik
authors.initials: YS
authors.lastname: Hong
authors.forename: Gil Hong
authors.initials: GH
authors.lastname: Park
authors.forename: Kyo Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D000468:Alkalies / Q000494:pharmacology; D000818:Animals; D049109:Cell Proliferation; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006498:Hepatectomy; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D006657:Histones / Q000494:pharmacology; D008115:Liver Regeneration / Q000187:drug effects / Q000502:physiology*; D008745:Methylation / Q000187:drug effects; D009687:Nuclear Proteins / Q000378:metabolism*; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D000468:Alkalies; D006657:Histones; D009687:Nuclear Proteins; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D012965:Sodium Chloride