Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice.
Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S1161, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S1161 transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.
Wang
Ai-Guo
AG
Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Moon
Hyung-Bae
HB
Lee
Young-Ho
YH
Yu
Seong-Lan
SL
Kwon
Hyun-Jung
HJ
Han
Ying-Hao
YH
Fang
Wan
W
Lee
Tae-Hoon
TH
Jang
Kyung-Lib
KL
Kim
Sang-Keun
SK
Yu
Dae-Yeul
DY
Lee
Dong-Seok
DS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006526:Hepatitis C / Q000473:pathology* / Q000821:virology; D006524:Hepatitis, Viral, Animal / Q000473:pathology* / Q000821:virology; D022781:Hepatocytes / Q000473:pathology / Q000821:virology; D008099:Liver / Q000473:pathology / Q000821:virology*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D019076:Transgenes; D014758:Viral Core Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus