title: System, trends and perspectives of proteomics in dicot plants Part II: Proteomes of the complex developmental stages.
abstract: This review is devoted to the proteomes of the complex developmental stages of dicotyledoneous (dicot) plant materials. The two core technologies, two-dimensional gel electrophoresis (2-DGE) and mass spectrometry (MS), independently or in combination with each other, are propelling dicot plant proteomics to new discoveries and functions, with the establishment of tissue-specific and organelle proteomes, mostly in Arabidopsis thaliana and Medicago truncatula, revealing their complexity and specificity. These experimental proteomes have provided a good start towards the establishment of high-density 2-DGE reference maps and peptide mass fingerprint databases, for not only the model dicot plants, A. thaliana and M. truncatula, but also other important dicot plants, which will serve as a basis for proteomes of many other dicot plants and plant materials.
authors.affiliation: Research Laboratory for Agricultural Biotechnology and Biochemistry (RLABB), G.P.O. Box 8207, Kathmandu, Nepal. gkagrawal@onebox.com
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.forename: Masami
authors.initials: M
authors.lastname: Yonekura
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Iwahashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwahashi
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D046913:Medicago truncatula / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D015388:Organelles / Q000235:genetics / Q000378:metabolism; D063245:Plant Development; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010944:Plants / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D020543:Proteome; D040901:Proteomics / Q000379:methods*
chemical_list: D010940:Plant Proteins; D020543:Proteome