Differential expression of Hyaluronic Acid Binding Protein 1 (HABP1)/P32/C1QBP during progression of epidermal carcinoma.
Our laboratory has characterized a novel cell surface glycoprotein, Hyaluronic Acid Binding Protein 1 (HABP1), interacting specifically with hyaluronan (HA) and regulating HA-mediated cellular event. The involvement of HA in different stages of carcinoma is well documented. In the present communication, the expression profile of HABP1 was investigated from initiation to progression of epidermal carcinoma in mice, induced by benzo[a]pyrene (B[a]P) exposure. During tumor initiation, HABP1 accumulated in inflammatory subsquamous tissue and with progression, the protein, was also seen to overexpress in papillomatic and acanthotic tissue. With the onset of metastasis, HABP1 overexpression was confined to metastatic islands, while it disappeared gradually from the surrounding mass. Such expression profiles in metastasized tissue were supported by decreased levels of HABP1, both at protein and transcript levels. These observations taken together suggest that the changes in HABP1 level coincide with specific stages of tumor progression, that lead to disruption of its interaction with HA, implying a role in the regulation of tumor metastasis.
Ghosh
Ilora
I
Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
Chowdhury
Anindya Roy
AR
Rajeswari
Moganty R
MR
Datta
Kasturi
K
D000818:Animals; D001564:Benzo(a)pyrene; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D024101:Mitochondrial Proteins; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D010212:Papilloma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011485:Protein Binding; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
C490193:C1qbp protein, mouse; D002273:Carcinogens; D018960:Hyaluronan Receptors; D024101:Mitochondrial Proteins; D001564:Benzo(a)pyrene; D006820:Hyaluronic Acid