Polymorphism of interleukin-10 promoter and tumor necrosis factor receptor II in Vietnamese patients with systemic lupus erythematosus.
The polymorphism of the interleukin-10 (IL-10) promoter and tumor necrosis factor receptor II (TNFRII) in Vietnamese patients with systemic lupus erythematosus (SLE) was examined by using the polymerase chain reaction (PCR) method with genomic DNA and allele-specific primers. In the frequency of IL-10 promoter 1082 genotypes consisting of AA, A/G and GG, the allele frequency of G in the SLE patients was significantly higher than that in the healthy controls. On the other hand, there was no statistical difference in the frequency of TNF receptor (TNFR) II 196 genotypes between the SLE patients and healthy controls. It was therefore suggested that the polymorphism of the IL-10 promoter, but not TNFRII, might participate in the pathogenesis of SLE in Vietnamese.
Khoa
Pham Dang
PD
Department of Microbiology and Immunology, Aichi Medical University School of Medicine, 480-1195 Nagakute, Aichi, Japan. yokochi@aichi-med-u.ac.jp
Sugiyama
Tsuyoshi
T
Yokochi
Takashi
T
D000293:Adolescent; D000328:Adult; D002648:Child; D003430:Cross-Sectional Studies; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000097:blood / Q000235:genetics*; D014744:Vietnam / Q000453:epidemiology
D005819:Genetic Markers; D047889:Receptors, Tumor Necrosis Factor, Type II; D016753:Interleukin-10; D004247:DNA