Optimum culture conditions for the production of N-substituted formamide deformylase by Arthrobacter pascens F164.
We investigated the optimum culture conditions for the production of a novel enzyme, N-substituted formamide deformylase, which acts mainly on N-benzylformamide, in Arthrobacter pascens F164. The highest enzyme activity was obtained when this strain F164 was cultivated in a synthetic medium with N-benzylformamide as sole nitrogen source. This deformylase was found to be an inducible enzyme depending on N-benzylformamide.
Fukatsu
Hiroshi
H
Institute of Applied Biochemistry, and Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
Goda
Masahiko
M
Hashimoto
Yoshiteru
Y
Higashibata
Hiroki
H
Kobayashi
Michihiko
M
D000581:Amidohydrolases / Q000096:biosynthesis*; D001173:Arthrobacter / Q000201:enzymology*; D003470:Culture Media / Q000737:chemistry; D004790:Enzyme Induction; D005559:Formamides / Q000378:metabolism
D003470:Culture Media; D005559:Formamides; D000581:Amidohydrolases; C493455:N-substituted formamide deformylase; C500252:N-benzylformamide