[Regulation of hepatic stellate cell activation by interleukin-10/platelet derived growth factor/mitogen-activated protein kinase pathway].
OBJECTIVE
To investigate the regulatory effect of interleukin-10 (IL10) on the activation of hepatic stellate cells (HSC) through platelet derived growth factor (PDGF) and mitogen-activated protein kinase (MAPK) pathways.


METHODS
HSC were divided randomly into 4 groups. Group 1 served as a control. HSC were incubated with 1 ng/ml, 5 ng/ml, and 25 ng/ml IL-10 in groups 2, 3 and 4. RT-PCR and western blot were used to detect the expression of PDGF and MAPK protein ERK and p38 and alpha-SMA.


RESULTS
Compared with the control group, expressions of ERK, p38 and alpha-SMA of groups 2, 3 and 4 were significantly lower (F values were 240.47, 21.39, 28.86 respectively. IL-10 inhibited PDGF and MAPK protein ERK and p38 and alpha-SMA expression in a dose-dependent way.


CONCLUSIONS
IL-10 inhibits activation of HSC through the PDGF/MAPK pathway.
Li
Tao
T
Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing 100044, China.
Leng
Xi-Sheng
XS
Qin
Zhi-Zhong
ZZ
Song
Sheng-Han
SH
Zhao
Li
L
Xiong
Liang-Fa
LF
Peng
Ji-Run
JR
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D016753:Interleukin-10 / Q000494:pharmacology*; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D051381:Rats; D015398:Signal Transduction
D010982:Platelet-Derived Growth Factor; D016753:Interleukin-10; D020928:Mitogen-Activated Protein Kinases