In vitro genotoxic and cytotoxic effects of protein somatic products from helminths on donor blood lymphocytes.
Protein somatic products of adult helminths Hymenolepis nana and Toxocara canis and secretory-excretory somatic product of Trichinella spiralis larvae in vitro produced genotoxic and cytotoxic effects on donor blood lymphocytes, which manifested in accumulation of single-chain breaks, alkaline-labile sites in nuclear DNA, and apoptotic cells. This effect depended on the concentration of parasitic protein products during coculturing.
Bekish
Vl Ya
VY
Department of Medical Biology and General Genetics, Vitebsk State Medical University, Moscow. bekishvl@tut.by
Durnev
A D
AD
D000328:Adult; D000818:Animals; D001782:Blood Donors; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D015801:Helminth Proteins / Q000633:toxicity*; D006801:Humans; D048189:Hymenolepis nana / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D017166:Toxocara canis / Q000378:metabolism*; D017160:Trichinella spiralis / Q000378:metabolism*
D015801:Helminth Proteins