Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves.
Poplar, whose genome is the first to be sequenced among woody plants, is a favorable model for plant biologists to enable them to understand molecular processes of growth, development and responses to environmental stimuli in trees. The sequence will allow the development of a strategy for improving environmental stress tolerance in forest trees. In this study, we have generated a full-length enriched cDNA library from leaves of axenically grown poplar (Populus nigra var. italica) subjected to environmental stress treatments by dehydration, high salinity, chilling, heat, abscisic acid (ABA) and H2O2. We sequenced >30,000 expressed sequence tags (ESTs) from the cDNA library and consequently collected approximately 4,500 non-redundant clones. We further analyzed cDNAs encoding an ERF/AP2-domain transcription factor which is specific in plants and plays an important role under stress. Thirteen candidates containing the ERF/AP2 domain were found within our EST resource. Some of them showed stress-responsive gene expression. We report here the first collection of full-length enriched stress-related ESTs of poplar and discuss environmental stress responses of forest trees in the light of comparative genomics.
Nanjo
Tokihiko
T
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687 Japan. nanjo@affrc.go.jp
Futamura
Norihiro
N
Nishiguchi
Mitsuru
M
Igasaki
Tomohiro
T
Shinozaki
Kazuo
K
Shinohara
Kenji
K
D000040:Abscisic Acid / Q000494:pharmacology; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics*; D003681:Dehydration / Q000235:genetics*; D020224:Expressed Sequence Tags / Q000737:chemistry*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016877:Oxidants / Q000494:pharmacology; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D032107:Populus / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D018076:DNA, Complementary; D016877:Oxidants; D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid