Humoral immune response against soluble and fractionate antigens in experimental sporotrichosis.
Sporotrichosis is a chronic granulomatous mycosis caused by the dimorphic fungus Sporothrix schenckii, which is widely distributed in nature, and presents a saprophytic mycelial form on plant debris and soil. The immunological mechanisms involved in the prevention and control of sporotrichosis are not yet fully understood. In this study, mice were studied after infection with Sporothrix schenckii. In the first week after infection, fungal loading increased and thence decreased drastically 14 days after infection. Analysis by immunoblotting showed that the sera of all mice tested had antibodies reacting only with a 70 kDa antigen, with predominance of IgG1 and IgG3. Taken together, our results show that antigens from S. schenckii induced a specific humoral response in infected mice.
Nascimento
Rosana C
RC
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, Bloco 17, 05508-900, São Paulo, Brazil.
Almeida
Sandro Rogério
SR
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D008099:Liver / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D013154:Spleen / Q000382:microbiology; D013175:Sporothrix / Q000254:growth & development / Q000276:immunology*; D013174:Sporotrichosis / Q000276:immunology* / Q000382:microbiology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G