Non-standard structures of the Ni-Fe cofactor in the regulatory and the NAD-reducing hydrogenases from Ralstonia eutropha.
Spectroscopy on two oxygen-insensitive Ni-Fe hydrogenases from Ralstonia eutropha (NAD-reducing, soluble hydrogenase; hydrogen sensor, regulatory hydrogenase) reveals non-standard catalytic behaviour and unique structures of their Ni-Fe cofactors. Possible mechanistic implications are briefly discussed.
Löscher
S
S
Freie Universität Berlin, FB Physik, Arnimallee 14, D-14195 Berlin, Germany.
Burgdorf
T
T
Buhrke
T
T
Friedrich
B
B
Dau
H
H
Haumann
M
M
D020118:Cupriavidus necator / Q000201:enzymology*; D006864:Hydrogenase / Q000737:chemistry* / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism
D010088:Oxidoreductases; C043296:nickel-iron hydrogenase; C021376:hydrogen dehydrogenase; D006864:Hydrogenase