[The culture of retinal muller cells and expression of its glutamate transporter GLAST].
OBJECTIVE
To culture retinal Müller cells (RMC) of infantile and mice in postnatal day 3 (P3) and study the expression of GLAST.


METHODS
RMCs of infantile and mice in P3 were cultured by tissue inoculation and shaken to allow detachment of other retinal cells. Then the morphologic and growth characteristics of the cells was oberseved every day. The second passage cells were identified with GFAP, Vimentin and the expression of GLAST was observed with immunofluorescent staining.


RESULTS
The cultured RMCs reached confluence in 15-20 days. These cells were positive in GFAP, Vimentin and GLAST antibody staining.


CONCLUSIONS
RMCs of infantile and mice in P3 can be successfully cultured by tissue inoculation, GLAST widely expresses on RMCs membrane.
Zhou
Xia
X
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China.
Xia
Xiaobo
X
Wang
Yuke
Y
Huang
Peigang
P
Liu
Shuangzhen
S
Xu
Xueliang
X
D027322:Amino Acid Transport System X-AG / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009457:Neuroglia / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000378:metabolism
D027322:Amino Acid Transport System X-AG