Oxidative stress responses during cassava post-harvest physiological deterioration.
A major constraint to the development of cassava (Manihot esculenta Crantz) as a crop to both farmers and processors is its starchy storage roots' rapid post-harvest deterioration, which can render it unpalatable and un-marketable within 24-72 h. An oxidative burst occurs within 15 min of the root being injured, that is followed by the altered regulation of genes, notably for catalase and peroxidase, related to the modulation of reactive oxygen species, and the accumulation of secondary metabolites, some of which show antioxidant properties. The interactions between these enzymes and compounds, in particular peroxidase and the coumarin, scopoletin, are largely confined to the vascular tissues where the visible symptoms of deterioration are observed. These, together with other data, are used to develop a tentative model of some of the principal events involved in the deterioration process.
Reilly
Kim
K
Department of Biology & Biochemistry, University of Bath, UK.
Gómez-Vásquez
Rocío
R
Buschmann
Holger
H
Tohme
Joe
J
Beeching
John R
JR
D000975:Antioxidants / Q000378:metabolism; D015152:Blotting, Northern; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000378:metabolism; D018556:Crops, Agricultural / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D002365:Manihot / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012603:Scopoletin / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D003374:Coumarins; D007527:Isoenzymes; D017382:Reactive Oxygen Species; D013481:Superoxides; C030123:coumarin; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D012603:Scopoletin