title: Follicular phase serum levels of luteinizing hormone do not influence delivery rates in in vitro fertilization cycles down-regulated with a gonadotropin-releasing hormone agonist and stimulated with recombinant follicle-stimulating hormone.
abstract: OBJECTIVE
  To assess the value of serum LH measurements in early and late follicular phase as predictors of ovarian response and IVF outcome in patients treated with recombinant FSH with GnRH agonist (GnRH-a) pituitary down-regulation.
  
  
  METHODS
  Retrospective cohort analysis.
  
  
  METHODS
  Institutional.
  
  
  METHODS
  Women undergoing 157 consecutive IVF cycles suppressed with leuprolide acetate (LA) started in the midluteal phase and stimulated with recombinant FSH. Only women <40 years of age and with a basal cycle day 3 serum FSH </=9 IU/L were included.
  
  
  METHODS
  Serum LH levels were measured on cycle days 3 (D3) and 10 (D10).
  
  
  METHODS
  Delivery rates. Other secondary outcome measures included fertilization rate, clinical pregnancy rate, and parameters of ovarian response (peak E(2), number of metaphase II oocytes, and number of ampules of recombinant FSH).
  
  
  RESULTS
  No significant differences were found with respect to ovarian response, fertilization rate, and outcome of pregnancy, when three threshold values of D3 and D10 serum LH (1, 1.5, and 2 mIU/mL) were analyzed. In addition, no significant differences were found between conception (n = 87) and no conception (n = 71) groups with respect to D3 or D10 LH. Receiver operator characteristic (ROC) analysis showed that neither the serum LH concentration on D3 nor on D10 was able to discriminate between conception and nonconception cycles (area under the curve [AUC](ROC)= 0.54, AUC(ROC)= 0.56), or between delivered pregnancies and first trimester pregnancy loss (AUC(ROC)= 0.53, AUC(ROC) = 0.61).
  
  
  CONCLUSIONS
  The suppressed levels of early and late follicular serum LH in women <40 years of age with normal ovarian function desensitized with a GnRH-a and treated with recombinant FSH are not predictive of ovarian response, pregnancy, or delivery. These data do not support the use of exogenous LH supplementation in this clinical scenario.
authors.affiliation: The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, Virgina 23507, USA.
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Cabrera
authors.forename: Laurel
authors.initials: L
authors.lastname: Stadtmauer
authors.forename: Jacob F
authors.initials: JF
authors.lastname: Mayer
authors.forename: William E
authors.initials: WE
authors.lastname: Gibbons
authors.forename: Sergio
authors.initials: S
authors.lastname: Oehninger
mesh_terms: D000328:Adult; D036861:Delivery, Obstetric; D015536:Down-Regulation; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005498:Follicular Phase / Q000097:blood*; D006801:Humans; D016729:Leuprolide / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology; D012189:Retrospective Studies
chemical_list: D011994:Recombinant Proteins; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D016729:Leuprolide