title: Type-specific reactivity of anti-glycoprotein G antibodies from herpes simplex virus-infected individuals is maintained by single or dual type-specific residues.
abstract: Glycoprotein G-1 (gG-1) of herpes simplex virus type 1 (HSV-1) and gG-2 of HSV-2 are the only known HSV proteins that induce type-specific human antibody responses. Recently, it was shown that purified human anti-gG-1 and anti-gG-2 antibodies presented a type-specific reactivity to immunogenic stretches with high similarity between gG-1 and gG-2. In this study, the molecular basis for this type-specific recognition was investigated employing synthetic peptides covering the indicated regions, including substitutions of the type-specific residues. The results revealed that single or dual type-specific residues localized within regions of high similarity could induce significant structural differences, explaining the type-specific recognition of the human antibody response to the gG proteins.
authors.affiliation: Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.|Department of Dermatovenereology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
authors.forename: Petra
authors.initials: P
authors.lastname: Tunbäck
authors.affiliation: Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergström
authors.affiliation: Department of Dermatovenereology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
authors.forename: Gun-Britt
authors.initials: GB
authors.lastname: Löwhagen
authors.affiliation: UPR9021 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
authors.forename: Johan
authors.initials: J
authors.lastname: Hoebeke
authors.affiliation: Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
authors.forename: Jan-Åke
authors.initials: JÅ
authors.lastname: Liljeqvist
mesh_terms: D019943:Amino Acid Substitution; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D006561:Herpes Simplex / Q000097:blood / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D010455:Peptides; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; C050591:glycoprotein gG-1, herpes simplex virus type 1