Secretoglobins in the human pituitary: high expression of lipophilin B and its down-regulation in pituitary adenomas.
Secretoglobins are small secreted proteins, the expression of which has mostly been associated with secretory mucosal epithelia. Several secretoglobins have been implicated in the development of various human cancers. Allelic deletions of chromosome 11q13 correlates with the invasiveness of pituitary tumors. Intriguingly, several secretoglobin genes are located on 11q13; however, for most of these genes the expression in the pituitary and pituitary tumors have not been investigated. Antibodies specific for the secretoglobin lipophilin B (SCGB1D2, BU101) were developed and used in an immunohistochemical analysis of a human normal tissue microarray. Prominent lipophilin B immunoreactivity was found in the secretory cells of the anterior pituitary. Eight of nine analyzed pituitary adenomas showed a reduction in lipophilin B immunoreactivity compared to normal pituitary. However, there was no apparent association between lipophilin B immunoreactivity and hormone production or tumor invasiveness. Expression of eight different secretoglobin mRNAs were analyzed in normal pituitary and the pituitary adenoma cell line HP75 by highly specific quantitative real-time reverse transcription-PCR assays. Lipophilins B and C (SCGB2A1, mammaglobin B) were the most prominently expressed secretoglobin mRNAs in the pituitary. No secretoglobin mRNA was detected in the HP75 cells. The present report demonstrates, for the first time, lipophilin B expression in the pituitary and its apparent down-regulation in pituitary adenomas.
Sjödin
Anna
A
Department of Radiation Sciences, Oncology, Umeå University, 901 87 Umeå, Sweden.
Guo
Dongsheng
D
Lund-Johansen
Morten
M
Krossnes
Bård Kronen
BK
Lilleng
Peer
P
Henriksson
Roger
R
Hedman
Håkan
H
D000236:Adenoma / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D060788:Mammaglobin B; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000378:metabolism*; D011510:Proteolipids / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D060765:Secretoglobins; D014598:Uteroglobin
D060788:Mammaglobin B; D009185:Myelin Proteins; D011510:Proteolipids; D012333:RNA, Messenger; C556464:SCGB1D2 protein, human; C556467:SCGB2A1 protein, human; D060765:Secretoglobins; D014598:Uteroglobin