Tissue localization and regulation by juvenile hormone of human allergen Bla g 4 from the German cockroach, Blattella germanica (L.).
The German cockroach, Blattella germanica (L.), produces several potent protein aeroallergens, including Bla g 4, a approximately 20 kDa lipocalin. RT-PCR, Northern analyses and in situ hybridization showed that Bla g 4 is expressed only in the adult male reproductive system. Western blotting and ELISA with rBla g 4 antiserum detected immunoreactivity in the utricles and the conglobate gland, but not in other tissues of the male reproductive system. The Bla g 4 protein content of males increased from adult emergence to day 14, but during copulation Bla g 4 was depleted in the male and transferred to the female within the spermatophore. Topical application of juvenile hormone III stimulated Bla g 4 production by both conglobate gland and utricles.
Fan
Y
Y
Department of Entomology and W. M. Keck Center for Behavioural Biology, North Carolina State University, Raleigh, NC 27695, USA.
Gore
J C
JC
Redding
K O
KO
Vailes
L D
LD
Chapman
M D
MD
Schal
C
C
D000367:Age Factors; D000485:Allergens / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D052179:Antigens, Plant; D015152:Blotting, Northern; D003058:Cockroaches / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005837:Genitalia, Male / Q000378:metabolism; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000378:metabolism*; D012737:Sex Factors
D000485:Allergens; D052179:Antigens, Plant; C097505:Blatella germanica allergen 4; D019476:Insect Proteins; D012333:RNA, Messenger; D012717:Sesquiterpenes; C036585:juvenile hormone III