Dopamine receptor 2 regulates L-type voltage-gated calcium channel in primary cultured mouse midbrain neural network.
1. Synchronous oscillation of intracellular Ca2+ in the central nervous system is essential for neural development. We previously reported that endogenous dopamine was involved with synchronous Ca2+ oscillation of primary cultured midbrain neurons, and that regulation of dopamine in synchronous oscillation was distinctly different through dopamine receptor 1 (D1R) and 2 (D2R): the action of dopamine through D1R or D2R was facilitative or suppressive, respectively, to the Ca2+ influx of synchronous oscillation. 2. In the present study, we confirmed that the suppressive effects of D2R were mediated by the regulation of the L-type voltage-gated Ca2+ channel, not by the regulation of NMDA receptor on the Ca2+ influx in the midbrain neural network showing synchronous oscillation. 3. This evidence promotes better understanding of the regulation of neural activity by endogenous dopamine in networked neurons.
Yasumoto
Fumie
F
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. aa37169@mail.ecc.u-tokyo.ac.jp
Negishi
Takayuki
T
Ishii
Yoshiyuki
Y
Kyuwa
Shigeru
S
Kuroda
Yoichiro
Y
Yoshikawa
Yasuhiro
Y
D000818:Animals; D020746:Calcium Channels, L-Type / Q000502:physiology*; D002478:Cells, Cultured; D065127:Dopamine D2 Receptor Antagonists; D005260:Female; D008636:Mesencephalon / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D020891:Raclopride / Q000494:pharmacology; D017448:Receptors, Dopamine D2 / Q000502:physiology*
D020746:Calcium Channels, L-Type; D065127:Dopamine D2 Receptor Antagonists; D017448:Receptors, Dopamine D2; D020891:Raclopride