Expression of GLUT12 in the fetal membranes of the human placenta.
The aim of this study was to characterize the expression of the novel glucose transporter GLUT12 in the fetal membranes of the human placenta. RT-PCR and Western blotting of extracts of amnion and choriodecidua from four normal term placentas identified GLUT12 mRNA and protein expression. In all four samples the signals for GLUT12 were markedly stronger in the choriodecidua than in the amnion, whereas the signals for GLUT1, a glucose transporter know to be expressed in fetal membranes, were similar for the two tissues. In further studies, paraffin sections of fetal membranes were analyzed by immunohistochemistry with GLUT12 and GLUT1-specific polyclonal antibodies. GLUT12 immunoreactivity was localized predominantly to the trophoblast cells in the chorion and to a lesser extent to decidual cells and to epithelial and fibroblast cells of the amnion. GLUT1 was localized to chorionic trophoblast cells and amniotic epithelial and fibroblast cells. GLUT12 expression was predominantly cytoplasmic, whereas GLUT1 was associated with the membrane of the cells. These results show that GLUT12 is expressed in cells of human fetal membranes and suggest that GLUT12 may play a role in the facilitation of glucose transport into these cells.
Gude
N M
NM
Department of Perinatal Medicine, Royal Women's Hospital, Carlton, Australia. neil.gude@rwh.org.au
Stevenson
J L
JL
Murthi
P
P
Rogers
S
S
Best
J D
JD
Kalionis
B
B
King
R G
RG
D000328:Adult; D015139:Blotting, Southern; D003593:Cytoplasm / Q000378:metabolism; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D051246:Glucose Transport Proteins, Facilitative; D006801:Humans; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D027342:Excitatory Amino Acid Transporter 2; D051246:Glucose Transport Proteins, Facilitative; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C449579:SLC2A12 protein, human