title: Evaluation of industrial, hospital and domestic wastewater genotoxicity with the Salmonella fluctuation test and the SOS chromotest.
abstract: An evaluation of the genotoxic potential of different wastewaters collected in the Rouen area was performed with the SOS chromotest (on Escherichia coli PQ37) and the Salmonella fluctuation test on Salmonella typhimurium strains TA98, TA100 and TA102 with or without metabolic activation. The samples were taken during two 1-week periods, one in January and one in April 2003. Six sites were selected for wastewater sampling in order to allow a comparative study between an area of mixed discharge (industrial, hospital and domestic) and an area of primarily domestic discharge. Out of a total of 71 daytime samples tested, 46 (65%) were positive in at least one assay: 22 samples out of 33 in January (67%), and 24 samples out of 38 in April (63%). The two genotoxicity tests have different sensitivities. Indeed, the Salmonella fluctuation test allowed the detection of 56% of the samples as genotoxic in January (18 out of 33), and 63% in April (24 out of 38) while the SOS chromotest allowed the detection of 18% of the samples as genotoxic, whatever the sampling period. The samples collected in domestic wastewater are at least as genotoxic as the samples collected in mixed wastewater. The possible source of the detected genotoxicity (industrial, hospital or domestic) is discussed. The results of this study show that the different types of wastewaters present a genotoxic risk. Additional studies should be undertaken in the analytical field in order to try to identify and quantify the compounds responsible for the genotoxicity. This difficult task will be necessary in order to identify the sources of toxicants and thus to take preventive and/or curative measures to limit the toxicity of the wastewater.
authors.affiliation: Laboratoire de Toxicologie, Faculté de Médecine et de Pharmacie, 22 Bd. Gambetta, F-76183 Rouen Cedex, France. bjolibois001@ch-compiegne.rss.fr
authors.forename: B
authors.initials: B
authors.lastname: Jolibois
authors.forename: M
authors.initials: M
authors.lastname: Guerbet
mesh_terms: D005602:France; D007220:Industrial Waste; D008506:Medical Waste; D009152:Mutagenicity Tests / Q000379:methods*; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism; D014865:Waste Disposal, Fluid; D014876:Water Pollution
chemical_list: D007220:Industrial Waste; D008506:Medical Waste