title: [Paternity analysis in deficiency cases with related putative fathers: simulation of a deficiency analysis in 27 families].
abstract: During the last few years, the number of privately ordered paternity investigations has increased considerably. Probably due to financial reasons in more and more cases only the putative father and the child are investigated. Additionally, very often only one method, such as STR analysis, is employed. This raises the question whether such a reduced analysis leads to reliable and clear results when investigating cases with related putative fathers. We investigated 165 individuals from 27 families using the AmpFlSTRIdentifiler multiplex PCR and calculated the paternity probabilities of the children to their biological fathers, uncles, grand fathers and other relatives. In more than 30% less than three exclusions between child and relative were detected. In five cases no exclusions were found between child and uncle, always leading to paternity probabilities >99.9%. These results show that the calculation of high probabilities (>99.9%) does not necessarily lead to the accurate conclusion of fatherhood. In many of our cases misleadingly the brother of the real father or another close relative would have been declared to be the biological father.
authors.affiliation: Aus dem Institut für Rechtsmedizin der Christian-Albrechts-Universität zu Kiel.
authors.forename: Nicole
authors.initials: N
authors.lastname: von Wurmb-Schwark
authors.forename: Victoria
authors.initials: V
authors.lastname: Mályusz
authors.forename: Eva
authors.initials: E
authors.lastname: Simeoni
authors.forename: Manfred
authors.initials: M
authors.lastname: Oehmichen
authors.forename: Eberhard
authors.initials: E
authors.lastname: Lignitz
authors.forename: Christina
authors.initials: C
authors.lastname: Lüdcke
authors.forename: Antje
authors.initials: A
authors.lastname: Repenning
authors.forename: Micaela
authors.initials: M
authors.lastname: Poetsch
mesh_terms: D041322:Chromosomes, Human, Y / Q000235:genetics; D003198:Computer Simulation; D016172:DNA Fingerprinting / Q000379:methods* / Q000706:statistics & numerical data; D005188:False Negative Reactions; D005220:Fathers / Q000145:classification / Q000706:statistics & numerical data; D005820:Genetic Testing / Q000379:methods* / Q000706:statistics & numerical data*; D005838:Genotype; D006801:Humans; D008297:Male; D008957:Models, Genetic; D015233:Models, Statistical; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics*