title: Analytical and semipreparative resolution of enatiomers of albendazole sulfoxide by HPLC on amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phases.
abstract: Broad spectrum anthelmintic agent-albendazole sulfoxide (ABZSO) have been separated and semiprepared on amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phases by HPLC using mobile phases contained with n-hexane and different alcohols. For analytical separation the influence of the nature and content of alcoholic modifiers on separation were systemically studied. Then, the analytical methods were scaled up to semipreparative loading to obtain small quantities (about 1 g) of both ABZSO enantiomers. Especially, different loading amounts were investigated for their effect on various parameters of semipreparative HPLC. In addition, optical rotation and circular dichroism (CD) of both ABZSO enantiomers collected were determined and single enantiomers were found stable in configuration for 1 year.
authors.affiliation: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. zzdlz@yahoo.com.cn
authors.forename: Zhai
authors.initials: Z
authors.lastname: Zongde
authors.forename: Luo
authors.initials: L
authors.lastname: Xingping
authors.forename: Wu
authors.initials: W
authors.lastname: Xiaomei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hong
authors.forename: Shi
authors.initials: S
authors.lastname: Yanping
authors.forename: Chen
authors.initials: C
authors.lastname: Liren
authors.forename: Li
authors.initials: L
authors.lastname: Yongmin
mesh_terms: D015766:Albendazole / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification*; D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D044367:Phase Transition; D048448:Phenylcarbamates / Q000737:chemistry*
chemical_list: D048448:Phenylcarbamates; C080033:Chiralpak AD; D000688:Amylose; D015766:Albendazole; C027186:albendazole sulfoxide