title: Potential application of a glucose-transport-deficient mutant of Schizosaccharomyces pombe for removing gluconic acid from grape must.
abstract: Musts from rotten grapes typically contain high levels of gluconic acid, which can raise severe problems in winemaking processes. In this work, the ability of the glucose-transport-deficient mutant YGS-5 of Schizosaccharomyces pombe to completely or partly remove gluconic acid from a synthetic glucose-containing medium and the potential use of this yeast strain for the same purpose in musts and wines were examined. Surprisingly, the S. pombe YGS-5 strain successfully removed 93% of the initial gluconic acid (2.5 gL(-1)) and 80% of the initial malic acid (1.0 gL(-1)) within 30 h after inoculation. Also, the yeast strain produced no volatile compounds other than those obtained in fermentations conducted with the wine yeast Saccharomyces cerevisiae. S. pombe YGS-5 could thus be used to remove gluconic acid present in musts from rotten grapes. On the basis of these results, various ways of using S. pombe YGS-5 to treat musts containing gluconic acid in order to solve the problems due to the high gluconic acid concentrations in botrytized grape must are proposed.
authors.affiliation: Departamento de Química Agrícola y Edafología, Edificio C-3, Universidad de Córdoba, Campus Universitario Rabanales, 14014 Córdoba, Spain.
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Peinado
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Moreno
authors.forename: Manuel
authors.initials: M
authors.lastname: Medina
authors.forename: Juan C
authors.initials: JC
authors.lastname: Mauricio
mesh_terms: D005285:Fermentation; D005511:Food Handling / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D005942:Gluconates / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000172:deficiency* / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics*; D027843:Vitis / Q000737:chemistry*; D014920:Wine
chemical_list: D005942:Gluconates; D009004:Monosaccharide Transport Proteins; C030691:gluconic acid