[Study on tissue culture of pasqueflower].
OBJECTIVE
To speed up seedling production of pasqueflower (Puzlsatilla chinenses) and their modernization in pasqueflower.


METHODS
With tissue culture method, primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment combinations of adding different hormones.


RESULTS
The appropriate medium for different culture stages were MS + 6-BA 1.0-3.0 mg x L(-1) + NAA 0-0.05 mg x L(-1) + Sucrose 30 g x L(-1) in primary culture, MS + 6-BA 0.2 mg x L(-1) + NAA 0.02 mg x L(-1) + BR 0.00001 mg x L(-1) + Sucrose 30 g x L(-1) in differentiation and subculture of cluster buds, 1/2 MS + NAA 0.4 mg x L(-1) + Sucrose 20 g x L(-1) in rooting.


CONCLUSIONS
Applying stem tip and flower buds as explants, high frequency propagation of seedlings can be achieved with plant tissue culture in Pasqueflower.
Zhang
Zi-xue
ZX
Anhui Technical Teacher College, Fengyang 233100 Anhui, China. zzxue2003@yahoo.com.cn
Ding
Wei-qun
WQ
Tang
Yong
Y
Shi
Wei-jing
WJ
Ye
Wen-cai
WC
D035264:Flowers / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development; D031946:Pulsatilla / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
D010937:Plant Growth Regulators