[Studies on anti-inflammatory and immunologic enhancement of kangning granules].
OBJECTIVE
To investigate the effect of Kangning granules on inflammatory and immunology.


METHODS
Pedal swelling induced by 1% agar in mice, dermal capillary hyper-permeability induced by histamine in rats were used to evaluate the anti-inflammatory of Kangning granules, and the clearance rate of intravenous charcoal particles was used to study its effect on non-specific immunologic functions. Serum haemolysin level and the number of antibody secretory cells in spleen were determined to assess its effects of specific immunologic functions. MTT colored method was used to determine the effect of Kangning granules on T- or B-Lymphocytes proliferation.


RESULTS
5, 10, 20 g x kg(-1) of Kangning Granules significantly inhibited pedal swelling induced by agar in mice (P < 0.05, P < 0.01). 2.4, 4.8, 9.6 g x kg(-1) of Kangning Granules evidently decreased dermal capillary hyper-permeability induced by histamine in rats (P < 0.05, P < 0.01). 10, 20 g x kg(-1) of Kangning Granules enhanced the phagocytic function and phagocytosing velocity in mice (P < 0.05). 5, 10, 20 g x kg(-1) of Kangning Granules obviously increased serum haemolysin level and the number of antibody secretory cells in spleen (P < 0.05, P < 0.01). 20 g x kg(-1) of Kangning Granules promoted B-Lymphocytes proliferation (P < 0.05).


CONCLUSIONS
Kangning granules significantly inhibited inflammatory reaction and increase immune functions in animals.
Wang
Yin-ye
YY
School of Pharmaceutical Sciences, Peking University, Beijing, 100083, China.
Liu
Xiao-yan
XY
Ai
Tie-min
TM
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D020900:Echinacea / Q000737:chemistry; D004487:Edema / Q000473:pathology; D005260:Female; D005534:Foot Diseases / Q000473:pathology; D007155:Immunologic Factors / Q000493:pharmacokinetics / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors