Optical imaging of neuronal populations during decision-making.
We investigated decision-making in the leech nervous system by stimulating identical sensory inputs that sometimes elicit crawling and other times swimming. Neuronal populations were monitored with voltage-sensitive dyes after each stimulus. By quantifying the discrimination time of each neuron, we found single neurons that discriminate before the two behaviors are evident. We used principal component analysis and linear discriminant analysis to find populations of neurons that discriminated earlier than any single neuron. The analysis highlighted the neuron cell 208. Hyperpolarizing cell 208 during a stimulus biases the leech to swim; depolarizing it biases the leech to crawl or to delay swimming.
Briggman
K L
KL
Division of Biological Sciences, University of California-San Diego, La Jolla, CA 92093-0357, USA.
Abarbanel
H D I
HD
Kristan
W B
WB
D000704:Analysis of Variance; D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000502:physiology; D004396:Coloring Agents; D003657:Decision Making; D016002:Discriminant Analysis; D004558:Electric Stimulation; D031541:Fluorescence Resonance Energy Transfer; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000502:physiology*; D007395:Interneurons / Q000502:physiology; D007865:Leeches / Q000166:cytology / Q000502:physiology*; D008124:Locomotion; D008564:Membrane Potentials; D008839:Microelectrodes; D009046:Motor Neurons / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D025341:Principal Component Analysis; D013550:Swimming
D004396:Coloring Agents