title: Immunolocalization of steroidogenic enzymes in the corpus luteum and placenta of the Japanese Shiba goat.
abstract: In this study, we performed immunohistochemistry of cholesterol side-chain cleavage cytochrome P450 (P450scc), 3beta-hydroxysteroid dehydrogenase (3betaHSD), cytochrome 17alpha-hydroxylase P450 (P450c17), and cytochrome P450 aromatase (P450arom) in the corpus luteum and placenta of Shiba goats. The aim was to clarify the steroidogenic capability of the corpus luteum and placenta of Shiba goats. Ovaries containing corpora lutea were obtained from four adult Shiba goats during the luteal phase (day10; n=2) and pregnancy (90 and 120 days of gestation). Placenta was obtained from one Shiba goat on day 120 of gestation. The sections of the ovaries and placentae were immunostained using the avidin-biotin-peroxidase complex method (ABC) with polyclonal antibodies generated against steroidogenic enzymes of mammalian origin. All luteal cells expressed P450scc, 3betaHSD, P450c17 and P450arom. The distribution of P450scc, 3betaHSD, P450c17 and P450arom were not different during the luteal phase and pregnancy. P450arom showed a weak positive staining in late pregnancy (120 days). In addition, immunoreactions for P450c17 and P450arom were observed in syncytiotrophoblast of the placenta of one Shiba goat. These results indicate that, in Shiba goats, corpus luteum is not only an important source of progesterone but also has the ability to synthesize androgen and estrogen during the luteal phase and pregnancy. Also the placenta has the ability to synthesize androgen and estrogen in late pregnancy.
authors.affiliation: Faculty of Biological Science and Technology, BeiJing Forestry University, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Medan
authors.forename: LongQuan
authors.initials: L
authors.lastname: Ren
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Toshio
authors.initials: T
authors.lastname: Tsubota
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D003338:Corpus Luteum / Q000201:enzymology*; D005260:Female; D006041:Goats / Q000502:physiology*; D007150:Immunohistochemistry; D008183:Luteal Phase / Q000502:physiology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism*
chemical_list: D011374:Progesterone; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D013250:Steroid Hydroxylases; D001141:Aromatase; D002786:Cholesterol Side-Chain Cleavage Enzyme