[Effect of puerarin on L-type calcium channel in isolated rat ventricular myocytes].
OBJECTIVE
To observe the effect of Puerarin on L-type calcium channel in isolated rat ventricular myocytes.


METHODS
The cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion techniques at constant flow rate. Whole-cell recording of patch-clamp techniques was used to observe the current of L-type calcium channel.


RESULTS
Puerarin 2.4 mmol x L(-1) could inhibit the current of L-type calcium channel of rat ventricular myocytes and this inhibition was time-dependent. Purerarin elevated the current-voltage (I-V) curve of calcium current.


CONCLUSIONS
Puerarin can inhibit L-type calcium current of rat ventricular myocytes. Which implies that puerarin takes part in anti-myocardial ischemia and anti-arrhythmics partly due to the inhibition of L-type calcium channel.
Guo
Xiao-gang
XG
Department of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. gxg@mail.hz.zj.cn
Chen
Jun-zhu
JZ
Zhang
Xiong
X
Xia
Qiang
Q
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000302:isolation & purification / Q000494:pharmacology*; D020746:Calcium Channels, L-Type / Q000187:drug effects*; D002469:Cell Separation; D006352:Heart Ventricles / Q000166:cytology; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D032383:Myocytes, Cardiac / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D010946:Plants, Medicinal / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D000889:Anti-Arrhythmia Agents; D020746:Calcium Channels, L-Type; D007529:Isoflavones; C033607:puerarin