Rapid G+ count and subpopulation assessment of the intestinal bacteria in Apodemus sylvaticus and Mus musculus by flow cytometry.
We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial population and to describe bacterial subpopulations in the intestine.
Moragues
M
M
Departament de Microbiologia, Universitat de Barcelona, 080 28 Barcelona, Spain.
Comas-Riu
J
J
Vives-Rego
J
J
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D015169:Colony Count, Microbial / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D007422:Intestines / Q000382:microbiology; D051379:Mice / Q000382:microbiology*; D009115:Muridae / Q000382:microbiology*; D009930:Organic Chemicals
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C461159:SYTO 13