Overexpression of small glutamine-rich TPR-containing protein promotes apoptosis in 7721 cells.
It is known that small glutamine-rich TPR-containing protein (SGT) is the member of TPR motif family. However, the biological functions of SGT remain unclear. In this paper, we report that SGT plays a role in apoptotic signaling. Ectopic expression of SGT enhances DNA fragment and nucleus breakage after the induction of apoptosis. Increasing mRNA level of SGT is also observed in 7721 cells undergoing apoptosis, knockdown the expression of endogenous SGT contributes to the decrease of apoptosis of 7721 cells. Deletion analysis reveals that TPR domain is critical to pro-apoptotic function of SGT. Furthermore, we demonstrated that the PARP cleavage and cytochrome c release are enhanced when SGT is overexpressed in 7721 cells during apoptosis. Collectively, our results indicate that SGT is a new pro-apoptotic factor.
Wang
Hanzhou
H
State Key Laboratory of Genetic Engineering & Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, People's Republic of China.
Shen
Hailian
H
Wang
Yanlin
Y
Li
Zejuan
Z
Yin
Hongyan
H
Zong
Hongliang
H
Jiang
Jianhai
J
Gu
Jianxin
J
D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D045304:Cytochromes c / Q000378:metabolism; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D018832:Molecular Chaperones; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*
D002352:Carrier Proteins; D018832:Molecular Chaperones; D011506:Proteins; C114575:SGTA protein, human; D045304:Cytochromes c; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases