title: Effect of HTK on the microcirculation in the rat cremaster muscle during warm ischemia and reperfusion.
abstract: Histidine-tryptophan-ketoglutarate (HTK) preserves rat muscle function during cold storage. We examined the effect of HTK perfusion on preservation of microvascular function during 4 h of warm ischemia and subsequent reperfusion (I/R) in the rat cremaster muscle. Leukocyte-endothelium interactions, capillary perfusion, and arteriole diameters were quantified prior to HTK-perfusion and/or ischemia, and at 0, 1, and 2 h after restoration of blood flow. In all groups, the number of rolling leukocytes increased with time, whereas I/R induced a slight increase in leukocyte adhesion. After ischemia, capillary perfusion rapidly recovered to about 50% and returned to near normal (90%) after 2 h. HTK at 22 degrees C did not affect the assessed microcirculation variables, whereas HTK at 4 degrees C reduced leukocyte rolling, but not adhesion. Therefore, microvascular function of HTK-perfused muscles was not better preserved during warm I/R than that of nonperfused muscles. Contrary to other preservation solutions, HTK perfusion in itself was not detrimental to the microcirculation.
authors.affiliation: Department of Plastic, Reconstructive and Hand Surgery, Utrecht University Medical Center, Utrecht, The Netherlands. jbas@spch.azm.nl
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Bastiaanse
authors.forename: Gary L
authors.initials: GL
authors.lastname: Anderson
authors.forename: Ralph J P M
authors.initials: RJ
authors.lastname: Franken
authors.forename: Brigitte E P A
authors.initials: BE
authors.lastname: van der Heijden
authors.forename: Mirjam G A
authors.initials: MG
authors.lastname: Oude Egbrink
authors.forename: Dick W
authors.initials: DW
authors.lastname: Slaaf
authors.forename: Moshe
authors.initials: M
authors.lastname: Kon
mesh_terms: D000818:Animals; D002450:Cell Communication; D004730:Endothelium, Vascular / Q000502:physiology; D005947:Glucose / Q000494:pharmacology*; D007962:Leukocytes / Q000502:physiology; D008297:Male; D008353:Mannitol / Q000494:pharmacology*; D008833:Microcirculation / Q000187:drug effects*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D011343:Procaine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control; D013524:Surgical Flaps / Q000098:blood supply*
chemical_list: C039984:Bretschneider cardioplegic solution; D019188:Organ Preservation Solutions; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose