Genetic diversity of Tunisian date palms (Phoenixdactylifera L.) revealed by nuclear microsatellite polymprohism.
Fourteen microsatellite loci of Phoenix dactylifera were targeted to examine the genetic diversity in Tunisian date-palms germplasm. They showed a high level of polymorphism in 49 accessions from three main oases with little geographic structure within Tunisia. The microsatellite data agrees with previous analyses of Tunisian germplasm using other molecular markers. 100% of local date-palms accessions were successfully fingerprinted and easily distinguished by the help of only three loci. The possibility of using microsatellites for large scale molecular labelling of offshoots and in vitro plantlets and their implication in the plant material certification is discussed.
Zehdi
Salwa
S
Laboratoire de Génétique Moléculaire, Immunologie & Biotechnologie, Faculté des Sciences de Tunis, Campus Universitaire, El Manar I, Tunis, Tunisia.
Trifi
Mokhtar
M
Billotte
Norbert
N
Marrakchi
Mohamed
M
Pintaud
Jean Christophe
JC
D028023:Arecaceae / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014416:Tunisia
D018744:DNA, Plant