Simultaneous determination of trans, transmuconic acid and s-phenylmercapturic acid by high pressure liquid chromatography and its application.
The simultaneous determination of urinary trans,trans-muconic acid (t,t-MA) and S-phenylmercapturic acid (S-PMA) was performed by liquid extraction with ethyl acetate and reversed-phase high performance liquid chromatography (RP-HPLC) on a Hypersil-ODS column using the gradient mobile phase of methanol and 0.0012 N perchloric acid and diode array detection at 205 and 264 nm for S-PMA and t,t-MA, respectively. The retention times for t,t-MA and S-PMA were 3.8 and 12.3 minutes, respectively. The recoveries of t,t-MA and S-PMA were > 97%; between-day precisions were all within 8% RSD (100x SD/mean). The method was applied to analyze the urinary t,t-MA and S-PMA of 59 service station attendants exposed to average benzene concentrations in the air of 0.20+/-0.18 ppm. Significant differences in pre-shift and post-shift urinary t,t-MA between smokers and non-smokers were found.
Tharnpoophasiam
Prapin
P
Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Kongtip
Pornpimol
P
Wongwit
Waranya
W
Fungladda
Wijitr
W
Kitayaporn
Dwip
D
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine*; D001554:Benzene / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D007221:Industry; D008297:Male; D016273:Occupational Exposure / Q000032:analysis*; D016272:Occupational Health; D010578:Petroleum; D012907:Smoking / Q000652:urine; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis*
D010578:Petroleum; C005939:muconic acid; C053003:S-phenyl-N-acetylcysteine; D003404:Creatinine; D001554:Benzene; D000111:Acetylcysteine; D013011:Sorbic Acid