Small Jab1-containing subcomplex is regulated in an anchorage- and cell cycle-dependent manner, which is abrogated by ras transformation.
Jab1 interacts with a variety of cell cycle and signal transduction regulators to control cell proliferation, differentiation, and tumorigenesis. In this study, we employed a non-denaturing gel electrophoresis method to separate different Jab1-containing complexes, the COP9 signalosome complex and the small Jab1-containing subcomplex. The formation of the small Jab1 complex was dependent on a low cell density and anchorage to a solid support, and enhanced during the early G1 phase of the cell cycle, which was abrogated in ras-transformed cells. The small Jab1-containing subcomplex may be a novel mediator of anchorage and cell-cell contact-dependent signal transduction.
Fukumoto
Akihisa
A
First Department of Surgery, Nara Medical University, Nara, Japan.
Tomoda
Kiichiro
K
Kubota
Makoto
M
Kato
Jun-ya
JY
Yoneda-Kato
Noriko
N
D000818:Animals; D000075686:COP9 Signalosome Complex; D002448:Cell Adhesion; D002452:Cell Count; D002453:Cell Cycle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003260:Contact Inhibition; D004268:DNA-Binding Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D046912:Multiprotein Complexes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010447:Peptide Hydrolases; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D046912:Multiprotein Complexes; D014157:Transcription Factors; D010447:Peptide Hydrolases; C501490:Cops5 protein, mouse; D000075686:COP9 Signalosome Complex; D018631:ras Proteins