title: Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
abstract: The MUC1 transmembrane mucin is expressed on the surface of activated human T cells; however, the physiologic signals responsible for the regulation of MUC1 in T cells are not known. The present studies demonstrate that IL-7, but not IL-2 or IL-4, markedly induces MUC1 expression on CD3+ T cells. MUC1 was also up-regulated by IL-15, but to a lesser extent than that found with IL-7. The results show that IL-7 up-regulates MUC1 on CD4+, CD8+, CD25+, CD69+, naive CD45RA+, and memory CD45RO+ T cells. In concert with induction of MUC1 expression by IL-7, activated dendritic cells (DC) that produce IL-7 up-regulate MUC1 on allogeneic CD3+ T cells. DC also induce MUC1 expression on autologous CD3+ T cells in the presence of recall Ag. Moreover, DC-induced MUC1 expression on T cells is blocked by a neutralizing anti-IL-7 Ab. The results also demonstrate that DC induce polarization of MUC1 on T cells at sites opposing the DC-T cell synapse. These findings indicate that DC-mediated activation of Ag-specific T cells is associated with induction and polarization of MUC1 expression by an IL-7-dependent mechanism.
authors.affiliation: Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Baldev
authors.initials: B
authors.lastname: Vasir
authors.forename: David
authors.initials: D
authors.lastname: Avigan
authors.forename: Zekui
authors.initials: Z
authors.lastname: Wu
authors.forename: Keith
authors.initials: K
authors.lastname: Crawford
authors.forename: Shawn
authors.initials: S
authors.lastname: Turnquist
authors.forename: Jian
authors.initials: J
authors.lastname: Ren
authors.forename: Donald
authors.initials: D
authors.lastname: Kufe
mesh_terms: D017951:Antigen Presentation; D002450:Cell Communication / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007156:Immunologic Memory; D015851:Interleukin-7 / Q000502:physiology*; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D018396:Mucin-1 / Q000096:biosynthesis* / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
chemical_list: D015851:Interleukin-7; D018396:Mucin-1; D017493:Leukocyte Common Antigens