Structure-aided optimization of kinase inhibitors derived from alsterpaullone.
In order to perform computer-aided design of novel alsterpaullone derivatives, the vicinity of the entrance to the ATP-binding site was scanned for areas that could be useful as anchoring points for additional protein-ligand interactions. Based on the alignment of alsterpaullone in a CDK1/cyclin B homology model, substituents were attached to the 2-position of the parent scaffold to enable contacts within the identified areas. Synthesis of the designed structures revealed three derivatives (3-5) with kinase-inhibitory activity similar to alsterpaullone. The novel 2-cyanoethylalsterpaullone (7) proved to be the most potent paullone described so far, exhibiting inhibitory concentrations for CDK1/ cyclin B and GSK-3beta in the picomolar range.
Kunick
Conrad
C
Institut für Pharmazeutische Chemie, Technische Universität Braunschweig, Beethovenstrasse 55, 38106 Braunschweig, Germany. c.kunick@tu-bs.de
Zeng
Zhihong
Z
Gussio
Rick
R
Zaharevitz
Daniel
D
Leost
Maryse
M
Totzke
Frank
F
Schächtele
Christoph
C
Kubbutat
Michael H G
MH
Meijer
Laurent
L
Lemcke
Thomas
T
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001552:Benzazepines / Q000737:chemistry* / Q000494:pharmacology; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D015195:Drug Design; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008958:Models, Molecular; D008968:Molecular Conformation; D047428:Protein Kinase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D018411:Spodoptera; D013329:Structure-Activity Relationship
D001552:Benzazepines; D007211:Indoles; D047428:Protein Kinase Inhibitors; C120793:alsterpaullone; D000255:Adenosine Triphosphate; D016203:CDC2 Protein Kinase