title: Effects of steroid hormones on catalase activity in neuronal and glial cell systems.
abstract: The aim of the present study was to determine short- (1 and 3 h) and long-term (24 h) effects of glucocorticoids [GCs; corticosterone (CORT), dexamethasone (DEX) and 6-methylprednisolone (6-MP)] and gonadal steroids [GSs; 17beta-estradiol (E2), progesterone (PROG) and testosterone (TEST)] on the activity of the hydrogen-peroxide-detoxifying enzyme catalase (CAT) in neural hippocampal HT22 cells and glial C6 cells because such effects have been described in peripheral organ systems. In HT22 cells, only long-term treatment with glucocorticoids (10(-5) M) induced effects on catalase activity, whereas gonadal steroids (10(-5) M) affected catalase activity after both short- and long-term incubations. At a lower concentration of 10(-7) M, glucocorticoids exerted only short-term treatment effects on catalase activity, while gonadal steroids (10(-7) M) affected the enzyme activity after short- and long-term treatments. In C6 glial cells, both glucocorticoids (10(-7) M) and gonadal steroids (10(-7) M) induced short- and long-term treatment effects. Thereby, our data show that steroid hormones differentially regulate catalase activity in models of the central nervous system (CNS) in a time- and steroid-dependent manner.
authors.affiliation: Department of Psychiatry and Psychotherapy, Neurobiological Laboratory, Philipps-University of Marburg, Rudolf-Bultmann-Str. 8; 35033 Marburg, Germany.
authors.forename: A J
authors.initials: AJ
authors.lastname: Schmidt
authors.forename: J-C
authors.initials: JC
authors.lastname: Krieg
authors.forename: H
authors.initials: H
authors.lastname: Vedder
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D005938:Glucocorticoids / Q000494:pharmacology*; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D051379:Mice; D009457:Neuroglia / Q000187:drug effects / Q000201:enzymology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D051381:Rats
chemical_list: D005938:Glucocorticoids; D012739:Gonadal Steroid Hormones; D002374:Catalase