N-acetylglucosaminyltransferase I activity of bovine oviduct epithelial cells: stimulation by luteinizing hormone, vascular endothelial growth factor and tumor necrosis factor alpha.
N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was found in media cultured with bovine oviduct epithelial cells (BOEC) obtained from non-pregnant cows during the follicular phase. Combined treatment with specific hormones increased GnT I release from BOEC. Luteinizing hormone (LH; 10 ng/ml) alone slightly, but together with 17beta-estradiol (E2; 1 ng/ml), synergistically increased GnT I activity. Vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF) alpha, which have been shown to have their highest activities in the bovine oviduct during the periovulatory period, also increased in GnT I activity. This study provides the first evidence of an increase of GnT I release from BOEC in vitro, and shows that endocrine as well as local factors such as LH, VEGF and TNFalpha increase this activity. The results suggest that GnT I activity in the bovine oviduct may contribute to the induction of glycosylation and thereby contributing to the provision of the optimal microenvironment for fertilization and early development of the embryos.
Nozaki
Hirofumi
H
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Wijayagunawardane
Missaka P B
MP
Kodituwakku
Suranga P
SP
Yoshida
Takashi
T
Nakamura
Tadashi
T
Arai
Ikichi
I
Urashima
Tadasu
T
Miyamoto
Akio
A
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005498:Follicular Phase / Q000502:physiology; D007986:Luteinizing Hormone / Q000494:pharmacology*; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D010057:Oviducts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D004958:Estradiol; D007986:Luteinizing Hormone; D017351:N-Acetylglucosaminyltransferases; C024850:alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I