Insulin-like growth factor-I alters apoptosis related genes and reduces apoptosis in porcine parthenotes developing in vitro.
The aim of this study was to determine the effects of IGF-I on the viability and development of porcine presumptive diploid parthenotes developing in vitro. In vitro derived 2-cell parthenotes were cultured to blastocysts with or without exogenous IGF-I in the presence or absence of BSA. The addition of 10 or 100 ng/ml IGF-I into NCSU 23 medium did not enhance the development of 2-cell parthenotes to the blastocyst stage. However, IGF-I increased cell numbers (P < 0.001) in the presence of BSA. Similarly, IGF-I alone did not affect apoptosis in the blastocysts but when combined with BSA, it reduced apoptosis (P < 0.05). Real time reverse-transcription polymerase chain reaction revealed that IGF-I significantly decreased the mRNA expression (pro-apoptotic gene Fas or Bak and anti-apoptotic gene Bcl-xL) ratios of both Fas/Bcl-xL (P < 0.01) and Bak/Bcl-xL (P < 0.01) in the presence of 0.4% BSA. These data suggest that IGF-I synergies with BSA to act as a mitogen and apoptotic survival factor in porcine preimplantation parthenotes.
Cui
Xiang-Shun
XS
Department of Animal Sciences, Chungbuk National University, Gaesin-dong, Cheongju, Chungbuk, 361-763, South Korea.
Jeong
Yu-Jeong
YJ
Jun
Jin-Hyun
JH
Kim
Nam-Hyung
NH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D002452:Cell Count; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D009865:Oocytes / Q000502:physiology; D010312:Parthenogenesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013552:Swine / Q000196:embryology*; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051020:bcl-X Protein; D019014:fas Receptor / Q000235:genetics
D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051020:bcl-X Protein; D019014:fas Receptor; D012710:Serum Albumin, Bovine; D007334:Insulin-Like Growth Factor I