[The effect of Baicalin on gene expression profile in rat brain of focal cerebral ischemia].
OBJECTIVE
To explore the difference of genes expression profiles between focal cerebral ischemia tissue and that treated with Baicalin using cDNA microarray.


METHODS
The total RNAs were isolated from rat brains of sham-operation, vehicle (focal cerebral ischemia of rat brain) and baicalin-treated groups. mRNAs were reversely transcribed to cDNA with incorporation of fluorescent dUTP (Cy5 or Cy3 dUTP) to prepare hybridization probes. The PCR products of 4096 genes were spotted on the chip after a serial of treatment. The mixed probes were hybridized to the cDNA microarray. Axon Genepix 4000B and GenePixPro 3.0 software were used to scan and analyze the fluorescent signals.


RESULTS
The expressions of 199 and 12 genes were found up-regulated and down-regulated, respectively, in the vehicle group compared with the sham-operation one. But the numbers of genes whose expressions were up-regulated and down-regulated were 89 and 88, respectively, when comparing the gene expression in the Baicalin-treated rat brain with that in the vehicle group. Moreover, one down-regulated and three up-regulated genes in the vehicle group were up-regulated and down-regulated in the Baicalin-treated group, respectively. Expressions of three up-regulated genes in the vehicle group were further reinforced in the Baicalin-treatment group.


CONCLUSIONS
Multiple pathways and nodes may be involved in the pharmacological effect of Baicalin on brain ischemia.
Wang
Zhong
Z
Xi Yuan Hospital of China Academy of Traditional Chinese Medicine, Beijing, 100091, China. zhonw@sina.com
Ying
Kang
K
Zhang
Zhan-jun
ZJ
Liu
Jian-xun
JX
Zhang
Xiao-yan
XY
Xu
Li
L
Wei
Cui-e
CE
Huang
Yan
Y
Wang
Yong-yan
YY
D000818:Animals; D002545:Brain Ischemia / Q000235:genetics* / Q000378:metabolism*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010946:Plants, Medicinal / Q000737:chemistry; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D031363:Scutellaria / Q000737:chemistry; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
D005419:Flavonoids; D012333:RNA, Messenger; D014746:Vimentin; C038044:baicalin; D011770:Pyruvate Kinase; D019204:GTP-Binding Proteins