title: The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies.
abstract: BACKGROUND: The permanently impaired protein folding during recombinant protein production resembles the stress encountered at extreme temperatures, under which condition the putative holding chaperones, IbpA/IbpB, play an important role. We evaluated the impact of ibpAB deletion or overexpression on stress responses and the inclusion body metabolism during production of yeast alpha-glucosidase in Escherichia coli. RESULTS: Deletion of ibpAB, which is innocuous under physiological conditions, impaired culture growth during alpha-glucosidase production. At higher temperatures, accumulation of stress proteins including disaggregation chaperones (DnaK and ClpB) and components of the RNA degradosome, enolase and PNP, was intensified. Overexpression of ibpAB, conversely, suppressed the heat-shock response under these conditions. Inclusion bodies of alpha-glucosidase started to disaggregate after arrest of protein synthesis in a ClpB and DnaK dependent manner, followed by degradation or reactivation. IbpA/IbpB decelerated disaggregation and degradation at higher temperatures, but did hardly influence the disaggregation kinetics at 15 degrees C. Overexpression of ibpAB concomitant to production at 42 degrees C increased the yield of alpha-glucosidase activity during reactivation. CONCLUSIONS: IbpA/IbpB attenuate the accumulation of stress proteins, and - at high temperatures - save disaggregated proteins from degradation, at the cost, however, of delayed removal of aggregates. Without ibpAB, inclusion body removal is faster, but cells encounter more intense stress and growth impairment. IbpA/IbpB thus exert a major function in cell protection during stressful situations.
authors.affiliation: Institute for Biotechnology, Department of Biochemistry/Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str, 3, D-06120 Halle, Germany. f.hoffmann@biochemtech.uni-halle.de.
authors.forename: Ha
authors.initials: H
authors.lastname: Lethanh
authors.forename: Peter
authors.initials: P
authors.lastname: Neubauer
authors.forename: Frank
authors.initials: F
authors.lastname: Hoffmann