title: Determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry.
abstract: We devised a sensitive and simple method for determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry. Bromide was alkylated with pentafluorobenzyl p-toluenesulphonate in the mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed using gas chromatography-mass spectrometry with the positive-ion EI mode. The lower limit of detection for the compound was 1 mg/l. The calibration curve for bromide was linear over the concentration range from 2 to 100 mg/l. With use of this method, levels of bromide in whole blood and urine were determined in cases of poisoning by inhaled brominated hydrocarbons.
authors.affiliation: Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, 7-7 Higashikoen, Hakata-ku, Fukuoka 812-8576, Japan.
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Kage
authors.forename: Keiko
authors.initials: K
authors.lastname: Kudo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ikeda
authors.forename: Akira
authors.initials: A
authors.lastname: Tsujita
authors.forename: Noriaki
authors.initials: N
authors.lastname: Ikeda
mesh_terms: D001565:Benzoates / Q000008:administration & dosage / Q000737:chemistry; D001965:Bromides / Q000097:blood* / Q000652:urine*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity; D005464:Fluorobenzenes; D005466:Fluorocarbons
chemical_list: D001565:Benzoates; D001965:Bromides; C090819:pentafluorobenzoic acid; D005464:Fluorobenzenes; D005466:Fluorocarbons