title: Semicarbazide-functionalized Si(111) surfaces for the site-specific immobilization of peptides.
abstract: The covalent attachment of semicarbazide-functionalized layers to hydrogen-terminated Si(111) surfaces is reported. The surface modification, based on the photoinduced hydrosilylation of a Si(111) surface with protected semicarbazide-functionalized alkenes, was investigated by means of X-ray photoelectron spectroscopy (XPS), contact angle measurements, and atomic force microscopy (AFM). The removal of the protecting group yielded a semicarbazide-terminated monolayer which was reacted with peptides bearing a glyoxylyl group for site-specific alpha-oxo semicarbazone ligation.
authors.affiliation: Institut d'Electronique et de Microélectronique et de Nanotechnologie, IEMN, (CNRS, UMR 8520) Département ISEN, 41 bd Vauban, 59046 Lille Cedex, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Coffinier
authors.forename: C
authors.initials: C
authors.lastname: Olivier
authors.forename: A
authors.initials: A
authors.lastname: Perzyna
authors.forename: B
authors.initials: B
authors.lastname: Grandidier
authors.forename: X
authors.initials: X
authors.lastname: Wallart
authors.forename: J-O
authors.initials: JO
authors.lastname: Durand
authors.forename: O
authors.initials: O
authors.lastname: Melnyk
authors.forename: D
authors.initials: D
authors.lastname: Stiévenard
mesh_terms: D004133:Diminazene / Q000031:analogs & derivatives* / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013053:Spectrophotometry; D014867:Water / Q000737:chemistry
chemical_list: D010455:Peptides; D014867:Water; C003915:diminazene aceturate; D004133:Diminazene; D012825:Silicon