[A multiplex for simultaneous amplification of three Y-STR loci using modified primers].
OBJECTIVE
To develop a multiplex method for simultaneous detection of Y-STR loci.


METHODS
The primers of Y-STR loci were devised for use in the simultaneous amplification of three Y-STR loci (DYS390, DYS391, DYS393).


RESULTS
Simultaneous amplification of the three Y-STR loci was successfully performed using the modified primers in which the fragment length of DYS391 was shortened from 279-287 bp to 142-150 bp, and at the same time, the virile specificity of DYS393 was enhanced.


CONCLUSIONS
The use of new-devised primers of Y-STR loci is a valid approach to rapid detection of multiple Y-STRs loci; it is worthy to be recommended in the field of forensic science.
Jia
Yi
Y
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Liu
Kuan-lin
KL
Zhou
Bin
B
Lü
Mei-li
ML
Liang
Wei-bo
WB
Zhang
Lin
L
D041322:Chromosomes, Human, Y; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics*
D017931:DNA Primers; D004247:DNA