[Anticancer effects of various fractions extracted from Dioscorea bulbifera on mice bearing HepA].
OBJECTIVE
To investigate the anti-cancer activities and the possible mechanism of Chinese herb Dioscrea bulbifera.


METHODS
The herb was extracted sequentially with petroleum ether, ethanol and water. The anticancer screen were carried out in vivo with HepA in mice.


RESULTS
The inhibitory effects on the formation of ascites volume and HepA cell viability in ascites were found in those extracted fractions except water fraction, the petroleum ether fraction being the strongest. Life span of mice bearing HepA ascites was prolonged after exposed to 100 mg x kg(-1) petroleum ether fraction and shortened after exposed to water fraction significantly. Besides, abnormal microstructure on HepA cells surface was found and it was supposed to be potential effect against viability of HepA which was convinced with the regeneration of HepA cells from ascites in mice exposed to petroleum ether fraction.


CONCLUSIONS
Anticancer active compounds are mainly extracted by petroleum ether from hydrophobic constituents of Dioscrea bulbifera and the anticancer effects were related to direct toxicity on tumor cell.
Yu
Ze-lan
ZL
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Liu
Xin-rong
XR
McCulloch
Michael
M
Gao
Jin
J
D000473:Alkanes; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D001201:Ascites / Q000473:pathology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D027742:Dioscorea / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol; D008113:Liver Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry
D000473:Alkanes; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D000431:Ethanol; C004544:naphtha