Sargassum hemiphyllum inhibits atopic allergic reaction via the regulation of inflammatory mediators.
Sargassum hemiphyllum (SH) has long been used in Korean folk medicine for the therapeutic treatment of various allergic diseases. The effects of SH in previous experimental models, however, have been inconclusive. We studied the effects of methanol extract of SH on mast cells. Our experiments showed that SH significantly inhibited compound 48/80-induced histamine and beta-hexosaminidase release from rat peritoneal mast cells. SH inhibited interleukin (IL)-8 and tumor necrosis factor (TNF)-alpha release induced by phorbol 12-myristate 13-acetate and A23187 from HMC-1, and it also showed an inhibitory effect on the anti-dinitrophenyl IgE antibody-induced passive cutaneous anaphylaxis reaction. In addition, SH inhibited the increase of TNF-alpha-induced NF-kappaB protein levels, transcription factor of TNF-alpha from 293T cells. A period of 48 h exposure to SH had little effect on HMC-1 cell viability. Our results suggest that SH has an inhibitory effect on the atopic allergic reaction and thus this may be useful in the treatment of allergic inflammatory diseases, such as atopic dermatitis.
Na
Ho-Jeong
HJ
College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Moon
Phil-Dong
PD
Ko
Seong-Gum
SG
Lee
Hee-Jung
HJ
Jung
Hyun-Ah
HA
Hong
Seung-Heon
SH
Seo
Youngwan
Y
Oh
Jay-Min
JM
Lee
Bong-Ho
BH
Choi
Byoung-Wook
BW
Kim
Hyung-Min
HM
D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D002460:Cell Line; D045744:Cell Line, Tumor; D003876:Dermatitis, Atopic / Q000276:immunology / Q000517:prevention & control*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016209:Interleukin-8 / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D007723:Korea; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008518:Medicine, East Asian Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D008517:Phytotherapy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D044647:Sargassum; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000037:antagonists & inhibitors / Q000378:metabolism
D018926:Anti-Allergic Agents; D018836:Inflammation Mediators; D016209:Interleukin-8; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D001619:beta-N-Acetylhexosaminidases