Dynamic changes in intranuclear and subcellular localizations of mouse Prrp/DAZAP1 during spermatogenesis: the necessity of the C-terminal proline-rich region for nuclear import and localization.
Mouse Prrp (mPrrp)/DAZAP1 is a mouse ortholog of Xenopus Prrp, which is involved in vegetal pole localization of Vg1 mRNA in oocytes and is highly expressed in the testis. The mouse protein has been reported to be a shuttling protein which localizes in the nucleus of pre-meiotic spermatogenic cells and round spermatids, and shifts its location into the cytoplasm in elongating spermatids, suggesting that mPrrp may be involved in mRNA transport as well as that of the Xenopus ortholog. We reexamined immunohistochemical analyses of mPrrp/DAZAP1 during spermatogenesis utilizing a newly established monoclonal antibody and reconfirmed it to be a shuttling protein. We also carried out new observations that included remarkable intranuclear movement during spermatogenesis. In addition, we found that a long amino acid stretch which spanned over the C-terminal half of the protein was required for the nuclear import. These observations demonstrated dynamic changes in subnuclear and subcellular localization which might reflect specific functions during spermatogenesis.
Kurihara
Yasuyuki
Y
Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, Hodogaya, Yokohama, Japan. kurihara@mac.bio.bsk.ynu.ac.jp
Watanabe
Hiroyuki
H
Kawaguchi
Atsushi
A
Hori
Tamaki
T
Mishiro
Kentaro
K
Ono
Michio
M
Sawada
Hajime
H
Uesugi
Seiichi
S
D021581:Active Transport, Cell Nucleus; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011392:Proline / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D013087:Spermatids / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism
D000911:Antibodies, Monoclonal; C412481:DAZAP1 protein, human; C510165:Dazap1 protein, mouse; C426830:PRLH protein, Xenopus; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins; D049452:Green Fluorescent Proteins; D011392:Proline