title: Complete sequence and gene organization of the Nosema spodopterae rRNA gene.
abstract: By sequencing the entire ribosomal RNA (rRNA) gene of Nosema spodopterae, we show here that its gene organization follows a pattern similar to the Nosema type species, Nosema bombycis, i.e. 5'-large subunit rRNA (2,497 bp)-internal transcribed spacer (185 bp)-small subunit rRNA (1,232 bp)-intergenic spacer (277 bp)-5S rRNA (114 bp)-3'. Gene sequences and the secondary structures of large subunit rRNA, small subunit rRNA, and 5S rRNA are compared with the known corresponding sequences and structures of closely related microsporidia. The results suggest that the Nosema genus may be heterogeneous and that the rRNA gene organization may be a useful characteristic for determining which species are closely related to the type species.
authors.affiliation: Department of Entomology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Shu-Jen
authors.initials: SJ
authors.lastname: Tsai
authors.forename: Wei-Fone
authors.initials: WF
authors.lastname: Huang
authors.forename: Chung-Hsiung
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016818:Nosema / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D033761:Spores, Protozoan / Q000235:genetics
chemical_list: D021901:DNA, Intergenic; D016054:DNA, Protozoan; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S