[Determination of hydromorphone in Beagle dogs plasma by liquid chromatography-tandem mass spectrometry].
OBJECTIVE
To establish a LC/MS/MS method for determination of hydromorphone (HYD) in Beagle dog plasma.


METHODS
After incubation with beta-glucuronidase for 16 h, an aliquot of 0.1 mL plasma was treated by liquid-liquid extraction. The analytes of interest were separated on a Zorbax SB C8 column with the mobile phase consisting of methanol-water- formic acid (65: 35: 1). Atmospheric pressure chemical ionization source of MS was applied and operated in positive ion mode.


RESULTS
The linear calibration curve was obtained in the concentration range of 0.80 - 200.0 microg x L(-1). The lower limit of quantification was 0.80 microg x L(-1). The inter-day and intra-day precision (RSD) was below 6.0%, and the accuracy (RE) was within 1% calculated from QC samples. The method was used to determine the pharmacokinetic parameters of HYD after a single oral administration of 4 mg HYD sustained release tablets to Beagle dogs.


CONCLUSIONS
The method was proved to be specific, sensitive, and suitable for the pharmacokinetic study of HYD sustained release formulation.
Li
Xiao-Yan
XY
Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, China.
Chen
Xiao-Yan
XY
Yan
Qing-Ying
QY
Zhang
Zhi-Hong
ZH
Xu
Jing-Hua
JH
Jin
Xin
X
Zhong
Da-Fang
DF
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003692:Delayed-Action Preparations; D004285:Dogs; D005260:Female; D004091:Hydromorphone / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D003692:Delayed-Action Preparations; D004091:Hydromorphone