title: Towards royal jelly proteome.
abstract: The recent availability of the honey-bee Apis mellifera genome and trascriptome of both the female castes, has stimulated new efforts in investigating the protein composition of royal jelly (RJ), its role in caste differentiation and its quality and typicality by a proteomic approach. This study is aimed both to separate and identify proteins of royal jelly and to detect some of them in honey-bee pollen-bread by using two-dimensional gel electrophoresis, mass spectrometry and by de novo sequencing. All the identified proteins belonged to the Apis mellifera genome. Apalbumin 1 was also confirmed to be present in honey-bee pollen-bread where the presence of apalbumin 2 was also found. In addition several fragments of apalbumin 1 and apalbumin 3 were also found in RJ. These could be the result of protease activity other than that of serine-protease. This study is a contribution to the description of royal jelly proteome.
authors.affiliation: Istituto di Biofisica del CNR, Pisa, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Scarselli
authors.forename: Elena
authors.initials: E
authors.lastname: Donadio
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Giuffrida
authors.forename: Donatella
authors.initials: D
authors.lastname: Fortunato
authors.forename: Amedeo
authors.initials: A
authors.lastname: Conti
authors.forename: Ettore
authors.initials: E
authors.lastname: Balestreri
authors.forename: Romano
authors.initials: R
authors.lastname: Felicioli
authors.forename: Mauro
authors.initials: M
authors.lastname: Pinzauti
authors.forename: Anna Gloria
authors.initials: AG
authors.lastname: Sabatini
authors.forename: Antonio
authors.initials: A
authors.lastname: Felicioli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000737:chemistry*; D005260:Female; D006722:Honey; D019476:Insect Proteins / Q000032:analysis*; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000099219:Royal Jelly
chemical_list: D005227:Fatty Acids; D019476:Insect Proteins; D020543:Proteome; D000099219:Royal Jelly