Novel development-related alternative splices in human testis identified by cDNA microarrays.
Alternative splicing of premessenger RNA is an important regulatory mechanism that increases the diversity of proteins transcribed from a single gene. This is particularly important in the testis because germ cell expansion and differentiation require many cellular changes and regulatory steps. To investigate novel development-related alternative splicings in the human testis, complementary DNA microarray studies were conducted with the use of probes from human fetal testes, adult testes, and human spermatozoa. Of a total of 386 Unigene clusters found to be related to the development of the testis, 67 clusters showed a total of 74 novel alternative spliceoforms. Developmental stage-dependent expression was also performed for a novel Unigene, NYD-SP20 (Hs.351068), which had 4 possible novel spliceoforms and another Unigene, CRISP2 (cysteine-rich secretory protein 2, Hs.2042), which had 3 possible novel spliceoforms. These results indicate that alternative splicing plays an important role in the complicated processes of testis development and spermatogenesis.
Huang
Xiaoyan
X
Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
Li
Jianmin
J
Lu
Li
L
Xu
Min
M
Xiao
Junhua
J
Yin
Lanlan
L
Zhu
Hu
H
Zhou
Zuomin
Z
Sha
Jiahao
J
D000328:Adult; D000368:Aged; D000375:Aging / Q000235:genetics*; D017398:Alternative Splicing; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000502:physiology*
D017931:DNA Primers; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger