A suitable Xylella fastidiosa CVC strain for post-genome studies.
The genome sequence of the pathogen Xylella fastidiosa Citrus Variegated Chlorosis (CVC) strain 9a5c has revealed many genes related to pathogenicity mechanisms and virulence determinants. However, strain 9a5c is resistant to genetic transformation, impairing mutant production for the analysis of pathogenicity mechanisms and virulence determinants of this fastidious phytopathogen. By screening different strains, we found out that cloned strains J1a12, B111, and S11400, all isolated from citrus trees affected by CVC, are amenable to transformation, and J1a12 has been used as a model strain in a functional genomics program supported by FAPESP (São Paulo State Research Foundation). However, we have found that strain J1a12, unlike strains 9a5c and B111, was incapable of inducing CVC symptoms when inoculated in citrus plants. We have now determined that strain B111 is an appropriate candidate for post-genome studies of the CVC strain of X. fastidiosa.
Teixeira
Diva do Carmo
Ddo C
Fundo de Defesa da Citricultura (Fundecitrus), Av. Dr. Adhemar Pereira de Barros, 201, 14807-040, V. Melhado-Araraquara, SP, Brazil. diva@fundecitrus.com.br
Rocha
Sanvai Regina Prado
SR
de Santos
Mateus Almeida
MA
Mariano
Anelise Galdino
AG
Li
Wen Bin
WB
Monteiro
Patricia Brant
PB
D032084:Citrus sinensis / Q000382:microbiology*; D003470:Culture Media; D016680:Genome, Bacterial; D009154:Mutation; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial; D014774:Virulence; D044167:Xylella / Q000235:genetics / Q000472:pathogenicity*
D003470:Culture Media