title: Factors influencing axillary shoot proliferation and adventitious budding in cedar.
abstract: We developed procedures for in vitro cloning of Cedrus atlantica Manetti and C. libani A. Rich explants from juvenile and mature plants. Explant size was one determinant of the frequency of axillary bud break in both species. Shoot tips and nodal explants mainly developed calli, whereas bud sprouting occurred in defoliated microcuttings cultured on a modified Murashige and Skoog medium without growth regulators. Isolation and continuous subculture of sprouted buds on the same medium allowed cloning of microcuttings from C. atlantica and C. libani seedlings and bicentennial C. libani trees, thus providing a desirable alternative for multiplying mature trees that have demonstrated superior characteristics. We also report adventitious bud differentiation from isolated embryos of C. atlantica. Neither auxin treatments nor other methods tested, including infection with Agrobacterium rhizogenes, were effective in inducing root initiation.
authors.affiliation: Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100-Burjasot (Valencia), Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Renau-Morata
authors.forename: Javier
authors.initials: J
authors.lastname: Ollero
authors.forename: Isabel
authors.initials: I
authors.lastname: Arrillaga
authors.forename: Juan
authors.initials: J
authors.lastname: Segura
mesh_terms: D028203:Cedrus / Q000254:growth & development*; D010937:Plant Growth Regulators; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics*; D012639:Seeds / Q000254:growth & development; D013696:Temperature; D046509:Tissue Culture Techniques
chemical_list: D010937:Plant Growth Regulators