Size-selective separation of gold nanoparticles using isoelectric focusing electrophoresis (IEF).
Isoelectric focusing in a polyacrylamide pH gradient gel is used to analyze the size distribution of gold nanoparticles synthesized by a chemical route with mercaptosuccinic acid as a ligand. The isoelectric point of the nanoparticles is shown to be size dependent, allowing fractionation by electrophoresis. Each fraction has a narrow size distribution with a standard deviation lower than 0.4 nm.
Arnaud
Isabelle
I
Ecole Polytechnique Fudurale de Lausanne, Laboratoire d'Electrochimie Physique et Analytique, 1015-Lausanne, Switzerland.
Abid
Jean-Pierre
JP
Roussel
Christophe
C
Girault
Hubert H
HH