title: [Protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline in vitro].
abstract: OBJECTIVE
  To investigate the cardio-protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline.
  
  
  METHODS
  After adding crocetin, the primary culture of cardiac myocyte was injured by 1.0 micromol x L(-1) noradrenaline. The activity of lactic dehydrogenase (LDH), mitochondrion succinic dehydrogenase (MSDH) and ATPase were assayed. The mitochondrion membrane potential was detected by Rh123. The percentage of cardiac myocyte apoptosis was observed by flow cytometry.
  
  
  RESULTS
  Crocetin significantly decreased the activity of LDH in culture supernatant, increased the activity of MSDH, ATPase (Na+-K+ ATPase, Ca2+ ATPase) and mitochondrion membrane potential.
  
  
  CONCLUSIONS
  Crocetin could alleviate the disturbance of energy metabolism and decrease the percentage of apoptosis of cardiac myocyte treated with noradrenaline.
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China. sxc9860@sina.com
authors.forename: Xiang-chun
authors.initials: XC
authors.lastname: Shen
authors.forename: Zhi-yu
authors.initials: ZY
authors.lastname: Qian
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Ya-juan
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002338:Carotenoids / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D027622:Crocus / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000201:enzymology*; D009638:Norepinephrine / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D014801:Vitamin A / Q000031:analogs & derivatives
chemical_list: D020011:Protective Agents; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D009638:Norepinephrine