title: Low-temperature growth of Shewanella oneidensis MR-1.
abstract: Shewanella oneidensis MR-1 is a mesophilic bacterium with a maximum growth temperature of approximately 35 degrees C but the ability to grow over a wide range of temperatures, including temperatures near zero. At room temperature ( approximately 22 degrees C) MR-1 grows with a doubling time of about 40 min, but when moved from 22 degrees C to 3 degrees C, MR-1 cells display a very long lag phase of more than 100 h followed by very slow growth, with a doubling time of approximately 67 h. In comparison to cells grown at 22 degrees C, the cold-grown cells formed long, motile filaments, showed many spheroplast-like structures, produced an array of proteins not seen at higher temperature, and synthesized a different pattern of cellular lipids. Frequent pilus-like structures were observed during the transition from 3 to 22 degrees C.
authors.affiliation: Department of Biological Sciences, 3651 Trousdale Pkwy., AHF 107, University of Southern California, Los Angeles, CA 90089-0371, USA.
authors.forename: Randa
authors.initials: R
authors.lastname: Abboud
authors.forename: Radu
authors.initials: R
authors.lastname: Popa
authors.forename: Virginia
authors.initials: V
authors.lastname: Souza-Egipsy
authors.forename: Carol S
authors.initials: CS
authors.lastname: Giometti
authors.forename: Sandra
authors.initials: S
authors.lastname: Tollaksen
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Mosher
authors.forename: Robert H
authors.initials: RH
authors.lastname: Findlay
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Nealson
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D003080:Cold Temperature; D008055:Lipids / Q000032:analysis; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D020592:Shewanella / Q000737:chemistry / Q000254:growth & development* / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D008055:Lipids