Immunosuppresive agents have an influence on ischemia-reperfusion injury in kidneys procured from a non-heart-beating donor: experimental study.
Ischemic-reperfusion injury (IRI) has a considerable influence on the function of kidneys from non-heart-beating donors (NHBD) after transplantation. IRI is accompanied by a marked inflammatory reaction with the production of reactive oxygen species and of the proinflammatory cytokine tumor necrosis factor alpha. The effect on the development of ischemic-reperfusion injury of early treatment of the donor with mycophenolate mofetil and tacrolimus was monitored in an animal model of a NHBD. The study demonstrated that the combination of the two immunosuppressives reduced the production in the NHBD kidney of tumor necrosis factor alpha, an indicator of the degree of inflammatory reaction after reperfusion, to a considerable extent but not of malondialdehyde or reduced glutathione. Pre-treatment of marginal donors with these immunosuppressants may improve the immediate function of transplanted kidneys by reducing cytokine production.
Treska
V
V
Department of Surgery, University Hospital, Plzen, Czech Republic. treska@fnplzen.cz
Molacek
J
J
Kuntscher
V
V
Liska
V
V
Kobr
J
J
Racek
J
J
Kormunda
S
S
D000818:Animals; D006323:Heart Arrest; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D023421:Models, Animal; D015427:Reperfusion Injury / Q000517:prevention & control*; D013552:Swine; D014019:Tissue Donors; D014184:Transplantation, Homologous / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007166:Immunosuppressive Agents; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde