Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification.
A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.
Ro
Hyeon-Su
HS
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea.
Jung
Sun Ok
SO
Kho
Byung Hoon
BH
Hong
Hyung Pyo
HP
Lee
Jae Sung
JS
Shin
Yong-Beom
YB
Kim
Min Gon
MG
Chung
Bong Hyun
BH
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D004950:Esterases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*
D011994:Recombinant Proteins; D006639:Histidine; D004950:Esterases