A universal method for automated gene mapping.
Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.
Zipperlen
Peder
P
Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. peder.zipperlen@molbio.unizh.ch
Nairz
Knud
K
Rimann
Ivo
I
Basler
Konrad
K
Hafen
Ernst
E
Hengartner
Michael
M
Hajnal
Alex
A
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D004331:Drosophila melanogaster / Q000235:genetics*; D017238:Genes, Helminth; D017344:Genes, Insect; D023281:Genomics; D005838:Genotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017384:Sequence Deletion; D020080:Tandem Repeat Sequences