Genomic allelotyping for distinction of recurrent and de novo hepatocellular carcinoma after orthotopic liver transplantation.
Distinction between recurrent and de novo hepatocellular carcinoma (HCC) after orthotopic liver transplantation (OLT) bears important clinical and therapeutic implications. Techniques for molecular profiling of clinically suspected de novo and recurrent HCC are required since the histological/clinical discrimination of donor vs. recipient tumor origin is difficult. Multiple PCR amplification of 16 highly polymorphic short tandem repeat (STR) DNA sequences (routinely used for paternity and forensic assays) was applied in two patients who developed a second HCC after OLT. In both patients the technique provided reliable evidence that the two second HCC were recurrences of the primary tumor. Multiple STR genetic allelotyping is an effective tool for clear-cut discrimination of donor/recipient origin of a second HCC after OLT. Its application could be of great therapeutic relevance for such OLT patients.
Altimari
Annalisa
A
Molecular and Transplantation Pathology Laboratory of the F. Addarii Institute of Oncology, Centro Riferimento Trapianti Emilia-Romagna, Bologna, Italy.
Gruppioni
Elisa
E
Fiorentino
Michelangelo
M
Petraroli
Rosella
R
Pinna
Antonio Daniele
AD
Petropulacos
Kyriakoula
K
Ridolfi
Lorenza
L
Costa
Alessandro Nanni
AN
Grigioni
Walter Franco
WF
Grigioni
Antonia D'Errico
AD
D000328:Adult; D000483:Alleles; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery*; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D004273:DNA, Neoplasm