[Experimental study on antivirus activity of traditional Chinese medicine].
OBJECTIVE
To investigate the difference of anticytomegaloviral activity of three kinds of traditional Chinese medicines which are the injections of Jinye Baidu, Radix Isatidis and Indigowoa in vitro.


METHODS
The inhibitory activity of three traditional Chinese medicines against human cytomeglovirus (HCMV AD169) infected human embryo lung fibroblasts (HELF) was observed by cytopathic effect method (CPE) and MTT method in vitro. According their value of A, anticytomegaloviral activity has evaluated.


RESULTS
Experimental study in vitro showed that the 50% toxicity dose (TD50) of Jinye Detoxifying, Radix Isatidis root and Indigowoa were 20, 10.23, 20.23 g x L(-1) respectively; the 50% inhibitory concertration (IC50) were 5.65, 3, 5.71 g x L(-1) respectively; the therapeutic index (TI) were 3.54, 3.41 and 3.54 respectively. It suggested that three traditional Chinese medicines had anticytomeglovirus activity and their effect increased with their concentration.


CONCLUSIONS
Three traditional Chinese medicines of the parenteral solution of Jinye Detoxifying, Radix Isatidis root and Indigowoa have antiviral activity when they are diluted in 1:200. They are safe and valuable drug for inhibiting cytomeglovirus infection.
He
Chao-man
CM
Department of Obstetrics and Gynecology, Affiliated Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wen
Liang-zhen
LZ
D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D020128:Inhibitory Concentration 50; D031219:Isatis / Q000737:chemistry*; D031239:Lonicera / Q000737:chemistry; D008168:Lung / Q000166:cytology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D032134:Saururaceae / Q000737:chemistry
D000998:Antiviral Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal