A simple, efficient, and economical method for recovering DNA from agarose gel.
A simple method of recovering DNA from agarose gel that is fast, inexpensive, and friendly both to operators and environment is described. Two rows of wells are made in an agarose gel, and a DNA sample is loaded into the well nearest to the negative pole for separation by electrophoresis. Recovery is accomplished by pipetting the DNA-containing TAE buffer from the well near the positive pole after target DNA fragments have migrated into the well. A recovery rate of up to 94 +/- 2.3% was observed with this method.
Fan
Chang-Fa
CF
Beijing Institute of Pharmacology and Toxicology, Beijing, PR China. fanchang@sohu.com
Mei
Xing-Guo
XG
D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005782:Gels / Q000032:analysis* / Q000737:chemistry*; D012685:Sepharose / Q000032:analysis* / Q000737:chemistry*; D014461:Ultracentrifugation / Q000379:methods
D005782:Gels; D004247:DNA; D012685:Sepharose