title: Lack of human herpesvirus 8 infection in lungs of Japanese patients with primary pulmonary hypertension.
abstract: Samples of lung tissue, taken at autopsy, from 10 Japanese patients with primary pulmonary hypertension (PPH) and samples of lung tissue from 12 Japanese patients with secondary pulmonary hypertension were tested for the presence of human herpesvirus 8 (HHV-8). All samples from patients with PPH contained plexiform lesions around pulmonary arterial vessels, but immunohistochemistry failed to detect the HHV-8-encoded latency-associated nuclear antigen. HHV-8 DNA could not be amplified by polymerase chain reaction for the HHV-8-encoded K1 and KS330(233) genes in any sample. These data suggest that HHV-8 infection is not associated with PPH in Japanese patients.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. katano@nih.go.jp
authors.forename: Harutaka
authors.initials: H
authors.lastname: Katano
authors.forename: Kinji
authors.initials: K
authors.lastname: Ito
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shibuya
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Saji
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000175:diagnosis*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D006976:Hypertension, Pulmonary / Q000821:virology*; D007150:Immunohistochemistry; D007223:Infant; D007564:Japan; D008168:Lung / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D009687:Nuclear Proteins; C109540:latency-associated nuclear antigen