Localization of sphingosine kinase-1 in mouse sperm acrosomes.
Sphingosine kinase (SPHK) catalyzes sphingosine phosphorylation to form a bioactive lipid mediator, sphingosine-1-phosphate (S1P). In the current study, we report the presence of SPHK-1 in mouse spermatozoa. SPHK-1 was localized to the acrosomes of spermatozoa, and its expression was proven by RT-PCR and Western blot analysis. SPHK activity of mouse spermatozoa was 18.1 pmol/min/mg protein. Furthermore, we identified the presence of the S1P receptors S1P1, S1P2, S1P3, and S1P5, in mouse spermatozoa by RT-PCR. These results suggest that S1P produced by SPHK-1 would play a role in the acrosomal reaction through S1P receptors.
Matsumoto
Kenji
K
Gifu International Institute of Biotechnology, Gifu, Japan. kmatsumo@giib.or.jp
Banno
Yoshiko
Y
Murate
Takashi
T
Akao
Yukihiro
Y
Nozawa
Yoshinori
Y
D000177:Acrosome / Q000201:enzymology*; D000818:Animals; D015153:Blotting, Western; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099157:Sphingosine Kinase
D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase