Effects of 24,25(OH)2D3, 1,25(OH)2D3 and 25(OH)D3 on alkaline and tartrate-resistant acid phosphatase activities in fetal rat calvaria.
The aim of this work was to evaluate the effects of 24,25-dihydroxyvitamin D3, 24,25(OH)2D3, on alkaline phosphatase (AP) and tartrate-resistant acid phosphatase (TRAP) activities in fetal rat calvaria cultures. These actions were compared with those of 1,25-dihydroxyvitamin D3, 1,25(OH)2D3, and 25-hydroxyvitamin D3, 25(OH)D3, in similar experimental conditions. At 10 min, 30 min and at 24 h incubation time, 1,25(OH)2D3 (10(-10)M) and 25(OH)D3 (10(-7) M) produced a significant increase in AP and TRAP activities compared to control group (without vitamin D metabolites). However, 24,25(OH)2D3 (10(-7) M) only produced effects on phosphatase activities similar to those produced by 1,25(OH)2D3 and 25(OH)D3, after 24 h incubation time. These findings suggest that 1,25(OH)2D3 and 25(OH)2D3 could carry out actions in minutes (nongenomic mechanism), while 24,25(OH)2D3 needs longer periods of time to perform its biological actions (genomic mechanism).
Municio
M J
MJ
Laboratorio de Bioquímica, Sección de Fisiopatología Osea, Fundación Jiménez Díaz, Avda. Reyes Católicos 2, 28040 Madrid, Spain.
Traba
M L
ML
D015650:24,25-Dihydroxyvitamin D 3 / Q000494:pharmacology*; D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002112:Calcifediol / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology*; D005333:Fetus; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D013644:Tartrates / Q000494:pharmacology
D013644:Tartrates; D015650:24,25-Dihydroxyvitamin D 3; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D002117:Calcitriol; D002112:Calcifediol