[Determination of aloin content in callus of Aloe vera var. chinensis].
The relationship between aloin accumulation of Aloe vera var. chinensis and the callus cultured by the roots, stems and leaves as explants. The aloin content in callus was determined by means of HPLC and TLC. The results showed that on the MS medium with NAA 1 mg/L + 6-BA 0.5 mg/L, the differentiation degree of the callus induced from the leaves was in the highest level, meanwhile the callus contained the most aloin. The aloin content was low in the callus from stems. There was no aloin in callus from roots. It was also found that on the MS medium with 2,4-D 1 mg/L + 6-BA 0.5 mg/L, the callus differentiation was in low level and without aloin, no matter what organs were used.
Wang
Hongxing
H
College of Life Science, Henan Normal University, Xinxiang 453002.
Li
Faqi
F
Wang
Taixia
T
Li
Jianjun
J
Li
Junying
J
Yang
Xiangfu
X
Li
Jingyuan
J
D000504:Aloe / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004642:Emodin / Q000031:analogs & derivatives* / Q000032:analysis; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media; D004365:Drugs, Chinese Herbal; D010937:Plant Growth Regulators; D004642:Emodin; C006457:alloin