Interstrand photocrosslinking of DNA via p-carbamoylvinyl phenol nucleoside.
We report a novel interstrand photocrosslinking of oligodeoxynucleotides (ODNs). In this system, a modified ODN containing p-carbamoylvinyl phenol nucleoside reacts by photoirradiation at 366 nm with adenine residue of a complementary template ODN to yield a crosslinked ODN in 97% yield.
Yoshimura
Yoshinaga
Y
PRESTO, Japan Science and Technology Agency, Ishikawa 923-1292, Japan. yosinaga@jaist.ac.jp
Ito
Yoshiaki
Y
Fujimoto
Kenzo
K
D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000528:radiation effects; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D008958:Models, Molecular; D008968:Molecular Conformation; D009705:Nucleosides / Q000737:chemistry* / Q000528:radiation effects; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects; D010636:Phenols / Q000737:chemistry* / Q000528:radiation effects; D013379:Substrate Specificity; D013696:Temperature
D003432:Cross-Linking Reagents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D010636:Phenols; D004247:DNA