title: Replication of an Egfr-wing shape association in a wild-caught cohort of Drosophila melanogaster.
abstract: Linkage disequilibrium mapping has been used extensively in medical and evolutionary genetics to map causal polymorphisms within genes associated with disease status or phenotypic variation for a trait. However, the initial findings of most nonhuman studies have not been replicated in subsequent studies, due in part to false positives, as well as additional factors that can render true positives unreplicable. These factors may be more severe when the initial study is performed using an experimental population of organisms reared under controlled lab conditions. We demonstrate that despite considerable phenotypic differences for wing shape between a lab-reared experimental population and a wild-caught cohort of Drosophila melanogaster, an association between a putative regulatory polymorphism in Egfr and wing shape can be replicated. These results are discussed both within the framework of future association-mapping studies and within the context of the evolutionary dynamics of alleles in populations.
authors.affiliation: Department of Genetics, North Carolina State University, Raleigh, 27695-7614, USA. i_dworkin@ncsu.edu
authors.forename: Ian
authors.initials: I
authors.lastname: Dworkin
authors.forename: Arnar
authors.initials: A
authors.lastname: Palsson
authors.forename: Greg
authors.initials: G
authors.lastname: Gibson
mesh_terms: D000483:Alleles; D000704:Analysis of Variance; D000818:Animals; D002874:Chromosome Mapping; D015331:Cohort Studies; D004331:Drosophila melanogaster / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism / Q000502:physiology*; D019143:Evolution, Molecular; D005787:Gene Frequency; D005838:Genotype; D015810:Linkage Disequilibrium; D008957:Models, Genetic; D010641:Phenotype; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014921:Wings, Animal / Q000033:anatomy & histology / Q000196:embryology*
chemical_list: D066246:ErbB Receptors