Quality control of roots of Eleutherococcus senticosus by HPLC.
An HPLC method based on several known methods for the determination of eleutherosides B and E was developed, optimised and validated in terms of linearity, precision (repeatability and intermediate precision on different days and at different concentration levels) and accuracy (recovery). The extraction procedure, the extraction solvent and the extraction yield were evaluated and optimised. A reversed-phase RP-18 column gradient eluted with a two-phase system consisting of phosphoric acid:water (0.5:99.5) and acetonitrile was used to evaluate the samples; detection was at 220 nm. Although eleutherosides B and E are commercially available, they are very costly, and therefore ferulic acid was chosen as external standard. The correction factors for the response of ferulic acid against both eleutherosides were determined and validated. This method, accepted by the European Pharmacopoeia Commission, will be included in the monograph on Eleutherococcus senticosus roots to assay the content of eleutherosides B and E.
Apers
Sandra
S
Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium. Sandra.Apers@ua.ac.be
Naessens
Tania
T
Van Miert
Sabine
S
Pieters
Luc
L
Vlietinck
Arnold
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028001:Eleutherococcus / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D017705:Lignans; D015394:Molecular Structure; D010666:Phenylpropionates / Q000737:chemistry; D028321:Plant Preparations / Q000592:standards; D018517:Plant Roots / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005960:Glucosides; D017705:Lignans; D010666:Phenylpropionates; D028321:Plant Preparations; C421885:eleutheroside E; C028305:syringin