title: Differences in stimulation of CD45+ cells from mouse spleen population enriched in dendritic cells depending on the virulence of herpes simplex virus type 1 strains.
abstract: The interaction of CD28 with one of the B7 molecules (CD80 and CD86) on professional antigen-presenting cells (APC) is generally considered to be the most important co-stimulatory signal for T cell activation. Several lines of evidence suggest that dendritic cells (DC), the most potent antigen presenting cells known, play a role in the immunological control of herpes simplex virus (HSV) infections. The fact that CD86 is strongly up-regulated together with other co-stimulatory molecules during DC maturation suggests that it plays an important role in induction of immune response. To determine the effect of virulence on up-regulation of CD86, we stimulated population of spleen cells enriched in dendritic cells by HSV-1 strains characterised by different pathogenicity. We analysed cells, which express CD45 molecule. HSV-1 ts, earlier described as less virulent for mice, stimulated an increased expression of co-stimulatory molecule CD86 than wild strain did.
authors.affiliation: National Institute of Hygiene, Department of Virology, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rechnio
authors.forename: Joanna
authors.initials: J
authors.lastname: Siennicka
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Gut
authors.forename: Bogumiła
authors.initials: B
authors.lastname: Litwińska
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D051940:B7-2 Antigen; D003713:Dendritic Cells / Q000502:physiology*; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D013154:Spleen / Q000276:immunology*; D014774:Virulence
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D008562:Membrane Glycoproteins; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1