Comparative assessment of genotyping methods for study genetic diversity of Fusarium oxysporum isolates.
In this study, we evaluated three PCR-based methods for the molecular typing of nonpathogenic Fusarium oxysporum isolates: random amplified polymorphic DNA (RAPD), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplified fragment length polymorphism (AFLP). The analyses were performed using 64 isolates of F. oxysporum collected from cotton-producing areas in Egypt. A number of polymorphic RAPD, PCR-RFLP and AFLP bands were scored in all isolates and the genetic similarity among them was assessed. Clustering analysis separated the isolates into two main groups, with similarities ranging from 87 to 100% for RAPD, 80 to 100% for PCR-RFLP and 88 to 97% for AFLP, respectively. The obtained data suggested that all three types of markers are equally informative, but the three assays differed in the amount of detected polymorphic bands. AFLP fingerprinting was also found to be more differentiating than other techniques for the typing of F. oxysporum populations.
Abd-Elsalam
Kamel A
KA
Agricultural Research Center, Plant Pathology Research Institute, Giza, Egypt. kaabdelsalam@msn.com
Guo
Jian-Rong
JR
Schnieder
Frank
F
Asran-Amal
Abdel-Mongy
AM
Verreet
Joseph-Alexander
JA
D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005670:Fusarium / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique
D021903:DNA, Ribosomal Spacer