title: Evidence for interspecific-recombination for three monopartite begomoviral genomes associated with the tomato leaf curl disease from central Sudan.
abstract: Two distinct viral genotypes were identified in the same tomato plant collected from Gezira, Sudan and are provisionally designated Tomato leaf curl Sudan virus (ToLCSDV-Gez) and Tomato yellow leaf curl virus-Sudan (TYLCV-SD). A third genotype was identified in tomato samples collected in Shambat, Sudan (ToLCSDV-Sha). The ToLCSDV-Gez and ToLCSDV-Sha isolates were approximately 90% identical, TYLCV-SD from Gezira shared approximately 93% identity with TYLCV-Mld. Recombination analyses identified two fragments in the ToLCSDV-Gez and TYLCV-SD genomes, providing evidence that these two genomes had undergone intermolecular recombination. A half unit size (737 nt) single-stranded satellite DNA was associated with ToLCSDV-Gez and TYLCV-SD.
authors.affiliation: Department of Plant Sciences, The University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Idris
authors.forename: J K
authors.initials: JK
authors.lastname: Brown
mesh_terms: D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013397:Sudan
chemical_list: D004276:DNA, Satellite; D004279:DNA, Viral