title: Cloning vectors based on cryptic plasmids isolated from lactic acid bacteria: their characteristics and potential applications in biotechnology.
abstract: Lactic acid bacteria (LAB) are Gram positive bacteria, widely distributed in nature, and industrially important as they are used in a variety of industrial food fermentations. The use of genetic engineering techniques is an effective means of enhancing the industrial applicability of LAB. However, when using genetic engineering technology, safety becomes an essential factor for the application of improved LAB to the food industry. Cloning and expression systems should be derived preferably from LAB cryptic plasmids that generally encode genes for which functions can be proposed, but no phenotypes can be observed. However, some plasmid-encoded functions have been discovered in cryptic plasmids originating from Lactobacillus, Streptococcus thermophilus, and Pediococcus spp. and can be used as selective marker systems in vector construction. This article presents information concerning LAB cryptic plasmids, and their structures, functions, and applications. A total of 134 cryptic plasmids collated are discussed.
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Shareck
authors.forename: Young
authors.initials: Y
authors.lastname: Choi
authors.forename: Byong
authors.initials: B
authors.lastname: Lee
authors.forename: Carlos B
authors.initials: CB
authors.lastname: Miguez
mesh_terms: D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D005516:Food Microbiology; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D005822:Genetic Vectors; D007778:Lactobacillus / Q000235:genetics*; D010957:Plasmids