[Flow injection chemiluminescent detection of acemetacin in KMnO4 - Na2 SO3 system].
OBJECTIVE
To study the sensitizing effect of acemetacin (ACE) on the weak chemiluminescent (CL) reaction of KMnO4 with sulfite and establish a fast and convenient method for CL detection of ACE.


METHODS
Using the sensitizing effect of ACE on KMnO4-Na2SO3 system and flow injection technique to determine the concentration of ACE.


RESULTS
Under optimal conditions, the CL intensity of 1.0 x 10(-2) mol x L(-1) H3PO4 - 5.0 x 10(-5) mol x L(-1) KMnO4 - 4.0 x 10(-4) mol x L(-1) Na2SO3 was proportional to the concentration of ACE ranging from 1.0 x 10(-7) to 1.0 x 10(-5) mol x L(-1). The detection limit of ACE was 6.9 x 10(-8) mol x L(-1) at 3sigma. Satisfactory results were obtained for determination of ACE at 2.5 x 10(-6) mol x L(-1).


CONCLUSIONS
The present method showed good precision, high sensitivity and selectivity and could be used for fast and convenient detection of ACE. It would be of significance to the clinical and pharmacological study of acemetacin.
Zhang
Zhi-Jie
ZJ
State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Nanjing University, Nanjing 210093, China.
Zhuang
Ya-Feng
YF
Ju
Huang-Xian
HX
D017022:Flow Injection Analysis / Q000379:methods*; D007213:Indomethacin / Q000031:analogs & derivatives* / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods*; D011196:Potassium Permanganate / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry
D013447:Sulfites; D011196:Potassium Permanganate; C026784:acemetacin; C025026:sodium sulfite; D007213:Indomethacin