Solid sampling technique for direct detection of condensed tannins in bark by matrix-assisted laser desorption/ionization mass spectrometry.
A novel method for the direct analysis of condensed tannin components in bark was developed on the basis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a simple solid sampling technique. The MALDI mass spectra obtained from the wood (bark) powder sample clearly showed a series of peaks corresponding to the sodium ion adducts of condensed tannin oligomers up to around m/z 3000. The results indicate that the condensed tannins in the bark sample used in this work mostly consist of combinations of flavan-3-ol units such as profisetinidin (PF), prorobinetinidin (PR) and prodelphinidin (PD), at least up to 10-mers (m/z approximately 3000).
Ishida
Yasuyuki
Y
Research Division of Energy Science, EcoTopia Research Institute, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan.
Kitagawa
Kuniyuki
K
Goto
Kuniyoshi
K
Ohtani
Hajime
H
D000045:Acacia / Q000378:metabolism*; D024301:Plant Bark / Q000378:metabolism*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013634:Tannins / Q000032:analysis* / Q000737:chemistry*; D014934:Wood
D010936:Plant Extracts; D013634:Tannins