title: Focused ion beam/scanning electron microscopy studies of Porcellio scaber (Isopoda, Crustacea) digestive gland epithelium cells.
abstract: The focused ion beam (FIB) was used to prepare cross sections of precisely selected regions of the digestive gland epithelium of a terrestrial isopod P. scaber (Isopoda, Crustacea) for scanning electron microscopy (SEM). The FIB/SEM system allows ad libitum selection of a region for gross morphologic to ultrastructural investigation, as the repetition of FIB/SEM operations is unrestricted. The milling parameters used in our work proved to be satisfactory to produce serial two-dimensional (2-D) cuts and/or three-dimensional (3-D) shapes on a submicrometer scale. A final, cleaning mill at lower ion currents was employed to minimize the milling artifacts. After cleaning, the milled surface was free of filament- and ridge-like milling artifacts. No other effects of the cleaning mill were observed.
authors.affiliation: Department of Biology, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobne
authors.forename: Marziale
authors.initials: M
authors.lastname: Milani
authors.forename: Alexis
authors.initials: A
authors.lastname: Zrimec
authors.forename: Maja Berden
authors.initials: MB
authors.lastname: Zrimec
authors.forename: Francesco
authors.initials: F
authors.lastname: Tatti
authors.forename: Kazimir
authors.initials: K
authors.lastname: Draslar
mesh_terms: D000818:Animals; D004848:Epithelium / Q000648:ultrastructure; D043143:Hepatopancreas / Q000648:ultrastructure*; D033321:Isopoda / Q000033:anatomy & histology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*