[Study on anti-HPV activity of Asarum heterotropoides].
OBJECTIVE
To screen anti-HPV effective fraction from Asarum heterotropoides.


METHODS
Asarum heterotropoides was extracted with systematic solvents (petroleum benzine, ether, ethyl acetate, n-butanol, ethanol and distilled water) accordingly. The Polymerase Chain Reaction (FQ-PCR) was applied in the vitro pharmacodynamics for the extracts from Asarum heterotropoides. The amplification of HPV-DNA in the lesions of the isolated Condyloma acuminatum.


RESULTS
After the water extracts of Asarum heterotropoides was used for HPV-DNA, the PCR amplification showed negative, with the minimum effective concentration 0.4 g/ml. But the other solvents extracts from Asarum heterotropoides appeared positive.


CONCLUSIONS
The water extracts from Asarum heterotropoides are effective on anti-Human papillomavirus.
Deng
Yuanhui
Y
Guangdong Hospital of TCM, Guangzhou 510120.
Feng
Yi
Y
Sun
Jing
J
Zhou
Dan
D
Yang
Liu
L
Lai
Jiaying
J
D000998:Antiviral Agents / Q000494:pharmacology*; D030023:Asarum / Q000737:chemistry*; D003218:Condylomata Acuminata / Q000821:virology; D004279:DNA, Viral / Q000187:drug effects / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D027383:Papillomaviridae / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods
D000998:Antiviral Agents; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal