Autologous heterotopic transplantation of ovarian tissue in sheep.
The aims of this study were [1] to test the feasibility of autologous heterotopic transplantation of large ovarian tissue samples (approximately 2 cm in diameter and 2 or 3 mm thick) without vascular anastomosis and [2] to compare follicle count and microvessel density in fresh and grafted 2-mm vs. 3-mm tissue samples in an animal model. After transplantation, we observed regular menstrual cycles and ovulation proved by high E(2) levels and intermittent P peaks in all Suffolk sheep, and histologic examination revealed no statistically significant differences in the number of primordial follicles and microvessel density between samples 2 mm and 3 mm in diameter.
Denschlag
Dominik
D
Department of Obstetrics and Gynecology, University of Freiburg, School of Medicine, Freiburg, Germany. ddenschl@frk.ukl.uni-freiburg.de
Knobloch
Christine
C
Kockrow
Andrea
A
Baessler
Anna
A
Goebel
Heike
H
Wellens
Eckhard
E
Haberstroh
Joerg
J
Tempfer
Clemens
C
Keck
Christoph
C
D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D005298:Fertility; D010053:Ovary / Q000637:transplantation*; D011374:Progesterone / Q000097:blood; D012756:Sheep; D014182:Transplantation, Autologous / Q000379:methods*
D011374:Progesterone; D004958:Estradiol