title: The role of antioxidant supplementation in cardiac transplantation: an experimental study in rats.
abstract: BACKGROUND
  We investigated three antioxidants, inositol hexaphosphate (IP6), superoxide dismutase (SOD), and catalase (CAT), using a Langendorf model of heart transplantation.
  
  
  METHODS
  Rat hearts were mounted on a Langendorf perfusion apparatus with addition of IP6, SOD+CAT, IP6+SOD+CAT to St. Thomas Hospital solution (n=6 for each) versus a control group (n=6), not containing supplementation. Global ischemia was achieved for 6 hours.
  
  
  RESULTS
  The worst peak-to-peak (PP) and +dp/dt maximum values were observed in the IP6+SOD+CAT group, the values being significantly lower than those in the SOD+CAT group. The lowest plasma creatine kinase (CK), CK-muscle-band (CK-MB), and lactate dehydrogenase levels were measured from the SOD+CAT group. The highest values for CK were in the control group, and those for CK-MB were in the IP6 group. The lowest myocardial malondialdehyde and adenosine triphosphate values were observed in the SOD+CAT group.
  
  
  CONCLUSIONS
  Supplementing St. Thomas Hospital solution with IP6 did not ameliorate myocardial damage following global ischemia. The contractility deteriorated further when IP6+SOD+CAT were used together; however, SOD+CAT improved cardiac mechanical functions, and significantly reduced myocardial damage.
authors.affiliation: Department of Cardiovascular Surgery, SureyyapasaThoracic and Cardiovascular Disease Hospital, Istanbul, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Kazimoglu
authors.forename: A K
authors.initials: AK
authors.lastname: Bozkurt
authors.forename: O
authors.initials: O
authors.lastname: Suzer
authors.forename: D
authors.initials: D
authors.lastname: Konukoglu
authors.forename: C
authors.initials: C
authors.lastname: Koksal
authors.forename: T
authors.initials: T
authors.lastname: Kurdal
authors.forename: M S
authors.initials: MS
authors.lastname: Turhan
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D016027:Heart Transplantation / Q000502:physiology*; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D007295:Inositol Phosphates; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D002374:Catalase; D013482:Superoxide Dismutase