[The modulation of pilose antler extract (PAE) on the proliferation of rat osteogenic cells UMR-106].
OBJECTIVE
To investigate the modulation of pilose antler extract (PAE) on rat osteogenic cells UMR-106 in vitro.


METHODS
Component P2 of PAE was isolated by Sephacryl S-200HR gel filtration chromatography. The proliferative effects of P2 and other components isolated by Sephacryl S-200HR on UMR-106 cells were investigated by MTT assay.


RESULTS
The P2 could significantly increase the proliferation rate of osteogenic cells. When the protein concentration of P2 was between 0.972 mg x L(-1) and 97.2 mg x L(-1), it could inhibit the proliferation of UMR-106 cells. But while the concentration was equal to or greater than 97.2 mg x L(-1), the P2 could increase the proliferation rate of cells, especially 477.92% at 9.72 g x L(-1), which was approximated to 499.62% of PAE. The molecular weight of the P2 was about 59 kDa determined by SDS-PAGE. On the other hand, inhibition was also observed in the sample of the P3, P4 and P5.


CONCLUSIONS
Those regulative factors in PAE which have different molecular weight can affect the proliferation of UMR-106 cells two-wayly. And this adjustment also relies on the dose of those factors. This finding may help us to understand the possible mechanism of Chinese traditional medicine from animal materials.
Chen
Xiao-chao
XC
Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China.
Ke
Li-jing
LJ
Chen
Gong-rui
GR
Liu
Shu-tao
ST
Huo
Yu-shu
YS
Rao
Ping-fan
PF
D000818:Animals; D000999:Antlers / Q000737:chemistry; D001859:Bone Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003670:Deer; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D012516:Osteosarcoma / Q000473:pathology*; D051381:Rats; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*
D008419:Materia Medica; D014020:Tissue Extracts