Rapid and efficient extraction of genomic DNA from different phytopathogenic fungi using DNAzol reagent.
A modified procedure using the commercial DNAzol reagent was successfully applied to extract genomic DNA from 25 fungal species. The DNA yield varied from 306 to 1,927 microg g(-1) dry mycelia and the A(260)/A(280) ratio from 1.59 to 1.93. Compared with the method of J.L. Cenis (Nucleic Acids Res. 1992, 20: 2380) this procedure generated a higher DNA yield from 17 species and a higher A(260)/A(280) ratio from 23 species. But for four species, Cenis (1992) method was more suitable. No inhibitor of polymerase chain reaction was evident for the DNA extracted by the modified procedure, whereas some inhibitors remained in DNA of eight species extracted by the previous method.
Guo
Jian-Rong
JR
Institute of Phytopathology, University of Kiel, Hermann-Rodewald-Str. 9, D-24118 Kiel, Germany. jguo@phytomed.uni-kiel.de
Schnieder
F
F
Abd-Elsalam
K A
KA
Verreet
J A
JA
D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005658:Fungi / Q000235:genetics*; D007202:Indicators and Reagents / Q000737:chemistry*
D017931:DNA Primers; D004271:DNA, Fungal; D007202:Indicators and Reagents