title: Internalization and trafficking mechanisms of coxsackievirus B3 in HeLa cells.
abstract: Coxsackievirus B3 (CVB3) is nonenveloped and has a single-stranded positive-sense RNA genome. CVB3 induces myocarditis and ultimately dilated cardiomyopathy. Although there are mounting evidences of an interaction between CVB3 particles and the cellular receptors, coxsackievirus and adenovirus receptor (CAR) and decay-accelerating factor (DAF), very little is known about the mechanisms of internalization and trafficking. In the present study, we used the CVB3 H3 strain, which is CAR-dependent but DAF-independent Woodruff variant and found that during entry, CVB3 particles were colocalized in clathrin, after interacting primarily with CAR, which was not recycled to the plasma membrane. We also found that CVB3 internalization was dependent on the function of dynamin, a large GTPase that has an essential role in endocytosis. Heat-shock cognate protein, Hsc70, which acts as a chaperone in the release of coat proteins from clathrin-coated vesicles (CCV), played a role in CVB3 trafficking processes. Moreover, endosomal acidification was crucial for CVB3 endocytosis. Finally, CVB3 was colocalized in early endosome autoantigen 1 (EEA1) molecules, which are involved in endosome-endosome tethering and fusion. In conclusion, these data together indicate that CVB3 uses clathrin-mediated endocytosis and is transcytosed to early endosomes.
authors.affiliation: Department of Biomedical Sciences, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul, 122-701 Korea.
authors.forename: Sun-Ku
authors.initials: SK
authors.lastname: Chung
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: In-Beom
authors.initials: IB
authors.lastname: Kim
authors.forename: Sang-Ick
authors.initials: SI
authors.lastname: Park
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Paek
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Nam
mesh_terms: D001483:Base Sequence; D002462:Cell Membrane / Q000502:physiology; D002966:Clathrin / Q000502:physiology; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D034281:Dynamins / Q000502:physiology; D004705:Endocytosis / Q000502:physiology; D011992:Endosomes / Q000502:physiology; D029822:Enterovirus B, Human / Q000502:physiology*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000502:physiology
chemical_list: C568771:CLMP protein, human; D002966:Clathrin; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D011991:Receptors, Virus; D034281:Dynamins