title: Preproinsulin-specific CD8+ T cells secrete IFNgamma in human type 1 diabetes.
abstract: In animal models autoreactive CD8(+) T cells are crucial in the development of type 1 diabetes (T1D); however, their role in human T1D is still not known. To address the role of CD81 T cells we performed a pilot study by investigating CD8(+) T cell-mediated cytokine secretion after in vitro stimulation with 94 preproinsulin (PPI) peptides. We were able to show that CD8(+) T cells contribute to a strong IFNgamma reactivity against PPI in human T1D. Further investigations defining epitope specificity, cytokine secretion, and cytotoxic capacity are important to clarify their role in T1D development.
authors.affiliation: Department of Internal Medicine I, Division of Endocrinology, University of Ulm, Robert-Koch Str. 8, 89081 Ulm, Germany.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rathmann
authors.forename: Tarvo
authors.initials: T
authors.lastname: Rajasalu
authors.forename: Silke
authors.initials: S
authors.lastname: Rosinger
authors.forename: Michael
authors.initials: M
authors.lastname: Schlosser
authors.forename: Thomas
authors.initials: T
authors.lastname: Eiermann
authors.forename: Bernhard O
authors.initials: BO
authors.lastname: Boehm
authors.forename: Ivana
authors.initials: I
authors.lastname: Durinovic-Belló
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005968:Glutamate Decarboxylase / Q000097:blood; D015789:HLA-A2 Antigen / Q000097:blood; D006683:HLA-DQ Antigens / Q000097:blood; D015803:HLA-DR3 Antigen / Q000097:blood; D015804:HLA-DR4 Antigen / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D010865:Pilot Projects; D011384:Proinsulin / Q000276:immunology*; D011498:Protein Precursors / Q000276:immunology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases / Q000097:blood; D054635:Receptor-Like Protein Tyrosine Phosphatases, Class 8; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015789:HLA-A2 Antigen; D006683:HLA-DQ Antigens; D015803:HLA-DR3 Antigen; D015804:HLA-DR4 Antigen; D007328:Insulin; D008565:Membrane Proteins; D010446:Peptide Fragments; D011498:Protein Precursors; D015847:Interleukin-4; C013737:preproinsulin; D007371:Interferon-gamma; D011384:Proinsulin; C516862:PTPRN protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases; D054635:Receptor-Like Protein Tyrosine Phosphatases, Class 8; C439756:GAD65 (217-236); D005968:Glutamate Decarboxylase