title: Identification of extracellular and intracellular signaling components of the mammary adipose tissue and its interstitial fluid in high risk breast cancer patients: toward dissecting the molecular circuitry of epithelial-adipocyte stromal cell interactions.
abstract: It has become clear that growth and progression of breast tumor cells not only depend on their malignant potential but also on factors present in the tumor microenvironment. Of the cell types that constitute the mammary stroma, the adipocytes are perhaps the least well studied despite the fact that they represent one of the most prominent cell types surrounding the breast tumor cells. There is compelling evidence demonstrating a role for the mammary fat pad in mammary gland development, and some studies have revealed the ability of fat tissue to augment the growth and ability to metastasize of mammary carcinoma cells. Very little is known, however, about which factors adipocytes produce that may orchestrate these actions and how this may come about. In an effort to shed some light on these questions, we present here a detailed proteomic analysis, using two-dimensional gel-based technology, mass spectrometry, immunoblotting, and antibody arrays, of adipose cells and interstitial fluid of fresh fat tissue samples collected from sites topologically distant from the tumors of high risk breast cancer patients that underwent mastectomy and that were not treated prior to surgery. A total of 359 unique proteins were identified, including numerous signaling molecules, hormones, cytokines, and growth factors, involved in a variety of biological processes such as signal transduction and cell communication; energy metabolism; protein metabolism; cell growth and/or maintenance; immune response; transport; regulation of nucleobase, nucleoside, and nucleic acid metabolism; and apoptosis. Apart from providing a comprehensive overview of the mammary fat proteome and its interstitial fluid, the results offer some insight as to the role of adipocytes in the breast tumor microenvironment and provide a first glance of their molecular cellular circuitry. In addition, the results open new possibilities to the study of obesity, which has a strong association with type 2 diabetes, hypertension, and coronary heart disease.
authors.affiliation: Department of Proteomics in Cancer, Institute of Cancer Biology, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark. jec@cancer.dk
authors.forename: Julio E
authors.initials: JE
authors.lastname: Celis
authors.forename: José M A
authors.initials: JM
authors.lastname: Moreira
authors.forename: Teresa
authors.initials: T
authors.lastname: Cabezón
authors.forename: Pavel
authors.initials: P
authors.lastname: Gromov
authors.forename: Esbern
authors.initials: E
authors.lastname: Friis
authors.forename: Fritz
authors.initials: F
authors.lastname: Rank
authors.forename: Irina
authors.initials: I
authors.lastname: Gromova
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000473:pathology*; D002457:Cell Extracts; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D007424:Intracellular Fluid / Q000378:metabolism*; D042361:Mammary Glands, Human / Q000166:cytology* / Q000601:surgery; D013058:Mass Spectrometry; D008408:Mastectomy; D017830:Octoxynol / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D012306:Risk
chemical_list: D000906:Antibodies; D002457:Cell Extracts; D020543:Proteome; D017830:Octoxynol