Database independent detection of isotopically labeled MS/MS spectrum peptide pairs.
Mass spectrometry data generated in differential profiling of complex protein samples are classically exploited using database searches. In addition, quantitative profiling is performed by various methods, one of them using isotopically coded affinity tags, where one typically uses a light and a heavy tag. Here, we present a new algorithm, ICATcher, which detects pairs of light/heavy peptide MS/MS spectra independent of sequence databases. The method can be used for de novo sequencing and detection of posttranslational modifications. ICATcher is distributed as open source software.
Potthast
Frank
F
Functional Genomics Center Zurich, Uni/ETH Zurich, Winterthurer Strasse 190, 8057 Zurich, Switzerland. frank@fgcz.ethz.ch
Ocenasek
Jiri
J
Rutishauser
Dorothea
D
Pelikan
Martin
M
Schlapbach
Ralph
R
D000465:Algorithms; D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*
D010455:Peptides