title: Emerging linezolid-resistant, vancomycin resistant Enterococcus faecium from a patient of a haematological unit in Poland.
abstract: The oxazolidinone antimicrobial, linezolid, has been approved for the treatment of infections caused by various gram-positive bacteria, including vancomycin-resistant enterococci (VRE). This is the first report on isolation of a linezolid resistant, vancomycin-resistant Enterococcus faecium (LRVREF) strain in Poland, from a Haematological Unit patient in the Clinical Hospital in Gdańsk. PCR-RFLP analysis of rDNA and allele-specific PCR of the domain V region of the 23S ribosomal RNA gene demonstrated the presence of the G2576U mutation previously reported to be associated with linezolid resistance. Both assays detected heterozygous in this position.
authors.affiliation: Department of Microbiology, Gdańsk University of Technology, ul G Narutowicza 11/12, 80-952 Gdańsk, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Krawczyk
authors.forename: Alfred
authors.initials: A
authors.lastname: Samet
authors.forename: Marek
authors.initials: M
authors.lastname: Bronk
authors.forename: Andrzej
authors.initials: A
authors.lastname: Hellmann
authors.forename: Józef
authors.initials: J
authors.lastname: Kur
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000328:Adult; D024881:Drug Resistance, Bacterial; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D000069349:Linezolid; D008297:Male; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020713:Vancomycin Resistance
chemical_list: D000081:Acetamides; D023303:Oxazolidinones; D000069349:Linezolid