title: B*4440: a novel HLA-B allele identified by sequence-specific oligonucleotide hybridization and sequence-specific amplification.
abstract: A novel human leukocyte antigen B (HLA-B) allele, B*4440, is described. The allele was identified in an adult stem cell donor of Caucasian origin. HLA-B*4440 most closely matches to B*4403 differing by a substitution of three nucleotides at codon 44, 45, and 50. Thus, low-resolution HLA typing using sequence-specific oligonucleotide hybridization or amplification using sequence-specific primers gave inconclusive results. DNA sequencing confirmed a variation of codons 44 and 45 (AGG AAG-->AGA GAG) and codon 50 (CCA-->CCG), resulting in an amino acid substitution Lys-->Glu at codon 45.
authors.affiliation: Institute of Transfusion Medicine, Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
authors.forename: G A
authors.initials: GA
authors.lastname: Heymann
authors.forename: B
authors.initials: B
authors.lastname: Hoppe
authors.forename: M
authors.initials: M
authors.lastname: Nagy
authors.forename: C
authors.initials: C
authors.lastname: Schoenemann
authors.forename: H
authors.initials: H
authors.lastname: Kiesewetter
authors.forename: A
authors.initials: A
authors.lastname: Salama
mesh_terms: D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013234:Stem Cells; D014019:Tissue Donors; D044465:White People
chemical_list: D015235:HLA-B Antigens