Phylogenetic analysis of classical swine fever virus in Taiwan.
Two envelope glycoprotein (Erns and E2) regions of the classical swine fever virus (CSFV) were amplified by RT-PCR and sequenced directly from 158 specimens collected between 1989 and 2003 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and the Erns region provided better discrimination than the E2 region. One hundred and fifteen isolates out of the 158 isolates were clustered within subgroup 2.1 (further classified as 2.1a and 2.1b) and 2.2, which were considered to be likely of the introduced strains, whereas the remaining 43 isolates were clustered within subgroup 3.4 and were considered to be of the endemic strains. The subgroup 2.1a viruses were first detected in 1994 and predominated from 1995 onwards. However, subgroup 3.4 viruses were prevalent in the early years, not being isolated after 1996. We have observed a dramatic switch in genotype from subgroup 3.4 to 2.1a. The subgroup 2.1a isolates are closely related to the Paderborn and Lao isolates, whereas 2.1b isolates have a close relationship to the Chinese Guangxi isolates. The phylogenetic tree of 27 CSFV sequences based on the complete envelope glycoprotein gene (Erns-E2) displayed better resolution than that based on the complete open reading frame.
Pan
C H
CH
Department of Hog Cholera, Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan.
Jong
M H
MH
Huang
T S
TS
Liu
H F
HF
Lin
S Y
SY
Lai
S S
SS
D000818:Animals; D006691:Classical Swine Fever / Q000453:epidemiology* / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013624:Taiwan / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus