title: [Studies on influence of fungal elicitor on hairy root of Panax ginseng biosynthesis ginseng saponin and biomass].
abstract: OBJECTIVE
  To study influence of fungal elicitors on the biomass and ginseng saponin biosynthesis of hairy roots of Panax ginseng (HRPG).
  
  
  METHODS
  Fungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV-spectrophotometry and RP-HPLC.
  
  
  RESULTS
  Fungal elicitors coula not only can influence on HRPG biomass and total ginseng sponin, but also improve or decrease some monomeric sponin. The total ginseng sponin could be increased to 3.649% but Rg1 and Re could not be detected when A. niger elicitors wss 20 mg x L(-1) in the culture fluid.
  
  
  CONCLUSIONS
  Fungal elicitor has specificity influence on secondary metabolite of HRPG. HRPG can biosynthesize specially active component by using specific fungal elicitor is used.
authors.affiliation: School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210038, China. firejj217@hotmail.com
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Jia-yi
authors.initials: JY
authors.lastname: Ding
authors.forename: Qian-yun
authors.initials: QY
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry / Q000502:physiology; D018920:Coculture Techniques; D005670:Fusarium / Q000737:chemistry / Q000502:physiology*; D036145:Ginsenosides / Q000096:biosynthesis*; D005894:Panax / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D036145:Ginsenosides; D011134:Polysaccharides; C049864:ginsenoside Re; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1