Isolation and partial characterization of equine herpesvirus type 1 in Czechia.
Equine herpesvirus type 1 was determined as the etiological cause of an abortion storm in Czechia in 2003 after the virus strain was isolated from aborted fetus and identified by serological means and by PCR technique. Cloning and sequencing of the glycoprotein D confirmed the identity of the isolates and showed molecular relationships to known EHV-1 strains. Comparison of glycoprotein D sequences with corresponding sequence of EHV-1 reference strains (Kentucky-A and Ab1) revealed high nucleotide homology. The Czech isolate of EHV-1 virus does not differ significantly from the Ab1 strain regarding the glycoprotein D gene and does not bear the frameshift in the 3' terminus which occurs in the Kentucky-A strain.
Molinková
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D
Institute of Microbiology and Immunology, Veterinary and Pharmaceutical University, 612 42 Brno, Czechia.
Celer
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Jahn
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D000034:Abortion, Veterinary / Q000453:epidemiology / Q000821:virology; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018153:Czech Republic / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology; D006736:Horses / Q000821:virology*; D008969:Molecular Sequence Data; D011247:Pregnancy; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1