[Preliminary study on hematopoietic constituents of si-wu-tang].
OBJECTIVE
To make preliminary study on the chemical foundation of hematopoietic effect of si-wu-tang.


METHODS
Si-wu-tang was fractionated by using precipitation method with ethanol and liquid-liquid extraction. Activity was investigated on radiated mice as model of blood deficiency.


RESULTS
At first si-wu-tang was separated into three fractions, fraction A, B and C. Activity study showed that fraction B and C could raise the amount of peripheral white blood cell in radiated mice. Then fraction B and C were respectively further separated into fraction B1, B2, B3 and fraction C1, C2, C3. Activity study showed that fraction C2, C3 and B3 could raise the amount of peripheral white blood cell in radiated mice. Furthermore, effect of fraction C1, C2, C3, B and paeoniflorin on hematopoietic progenitor cell in bone marrow of radiated mice were investigated. Fraction C2, C3, B and paeoniflorin could promote the proliferation of hematopoietic progenitor cell in bone marrow of radiated mice.


CONCLUSIONS
Fraction C2, C3, B and paeoniflorin are responsible for hematopoietic activity of si-wu-tang.
Liang
Qian-de
QD
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Lu
Xiao-qin
XQ
Ma
Zeng-chun
ZC
Tan
Hong-ling
HL
Ma
Bai-ping
BP
Gao
Yue
Y
Wang
Sheng-qi
SQ
D029971:Angelica sinensis / Q000737:chemistry; D000818:Animals; D001565:Benzoates / Q000302:isolation & purification / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000528:radiation effects*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007958:Leukocyte Count; D030004:Ligusticum / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry; D014916:Whole-Body Irradiation; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C403258:si-wu-tang; C015423:peoniflorin; D006027:Glycosides