title: Functional proteomics for the discovery of carbohydrate-related enzyme activities.
abstract: Carbohydrate metabolism is central to the growth and development of organisms. Thousands of genes that are believed to code for proteins that build and remodel saccharides have been uncovered by genome sequencing projects, thereby necessitating higher-throughput methods to delineate the chemical and biological functions of all these proteins. Recent methods discussed in this review have begun to address this problem by the design of new activity-based probes and mass-differentiated carbohydrate libraries. In addition, a comparative view of carbohydrate-related enzymes from all three branches of life has led to the discovery of unusual enzymes to simplify the synthesis of carbohydrates such as the sugar nucleotides required by glycosyltransferases.
authors.affiliation: Department of Chemistry and the Plant Sciences Institute, Iowa State University, Ames, IA 50011-3111, USA. npohl@iastate.edu
authors.forename: Nicola L
authors.initials: NL
authors.lastname: Pohl
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D040901:Proteomics
chemical_list: D002241:Carbohydrates; D004798:Enzymes