Uniformly sized molecularly imprinted polymer for atropine and its application to the determination of atropine and scopolamine in pharmaceutical preparations containing Scopolia extract.
A uniformly sized molecularly imprinted polymer (MIP) for atropine has been prepared. The MIP was prepared using 2-(trifluoromethyl) acrylic acid and ethylene glycol dimethacrylate as a functional monomer and cross-linker, respectively, by a multi-step swelling and thermal polymerization method. The selectivity factor, which is defined as the ratio of the retention factors (k) on the molecularly imprinted and non-imprinted polymers, k(imprinted)/k(non-imprinted), was 2.2 for atropine on the MIP. The obtained MIP was applied for the determination of tropane alkaloids (atropine and scopolamine) in a commercial gastrointestinal drug by a column-switching HPLC system, consisting of an MIP material as a pre-column, and a conventional cation-exchange analytical column. An interference peak was observed at the retention time of atropine derived from pre-column. However, since the peak area was less than 0.5% the peak area of atropine of a standard solution under the analytical conditions of this study (0.2 microg of atropine was loaded), this interference was negligible in the determination of atropine. On the other hand, no interference peak was observed at the retention time of scopolamine. Calibration curves of atropine and scopolamine showed good linearity in the range of 0.02-0.9 microg/ml (r=0.9999) and 0.003-0.09 microg/ml (r=0.9998), respectively. The mean recoveries of atropine and scopolamine from a placebo pharmaceutical preparation sample were 98.9 and 99.9%, respectively. The intra-day precision (measured by relative standard deviation, R.S.D. (%)) of both ingredients was less than 2.0%. The optimized column-switching system was applied successfully to the determination of atropine and scopolamine in a commercial gastrointestinal drug.
Nakamura
Masanori
M
Taisho Pharmaceutical Co. Ltd., 403 Yoshino-cho 1-chome, Kita-ku, Saitama-shi, Saitama 331-9530, Japan. masanori.nakamura@po.rd.taisho.co.jp
Ono
Masaki
M
Nakajima
Toshiaki
T
Ito
Yuji
Y
Aketo
Takao
T
Haginaka
Jun
J
D000179:Acrylates / Q000737:chemistry; D001285:Atropine / Q000302:isolation & purification*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D005765:Gastrointestinal Agents / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012015:Reference Standards; D012601:Scopolamine / Q000302:isolation & purification*; D032603:Scopolia / Q000737:chemistry*; D012680:Sensitivity and Specificity
C411824:2-(trifluoromethyl)acrylic acid; D000179:Acrylates; D003432:Cross-Linking Reagents; D005765:Gastrointestinal Agents; D008689:Methacrylates; D010936:Plant Extracts; D011108:Polymers; C004919:ethylene dimethacrylate; D001285:Atropine; D012601:Scopolamine