G protein threshold behavior in the human neutrophil oxidant response: measurement of G proteins available for signaling in responding and nonresponding subpopulations.
Threshold behavior is an important aspect of signal transduction pathways that allows for responses to be turned on or off. Human neutrophil responses to N-formyl peptides, including oxidant production and release, exhibit threshold behavior with respect to the number of G proteins available for signaling; progressive treatment of neutrophils with pertussis toxin causes the conversion of responding cells to nonresponding cells. To quantify the threshold level of G proteins required for signaling of N-formyl peptide stimulated oxidant production in a neutrophil population, we used a plasma membrane associated G protein quantification assay in conjunction with a sorting flow cytometer and measured differences in the average number of G proteins available for signaling per cell in both the responding and the nonresponding subpopulations after pertussis toxin treatment. Although there appeared to be a threshold separating responding cells and nonresponding cells for a given sample, no discrete threshold was measured across multiple treatment conditions. A mathematical model of the early steps in signaling suggests that cell-to-cell variability in signal parameters, such as numbers of signal components and values of kinetic rate constants, obscures the measurement of a discrete threshold and leads to an apparent decrease in the threshold level of G proteins available for signaling as the total G proteins are decreased.
Chang
Peter S
PS
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Axelrod
Daniel
D
Omann
Geneva M
GM
Linderman
Jennifer J
JJ
D002469:Cell Separation; D005434:Flow Cytometry; D020962:Heterotrimeric GTP-Binding Proteins / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism*; D015398:Signal Transduction
D016877:Oxidants; D009240:N-Formylmethionine Leucyl-Phenylalanine; D020962:Heterotrimeric GTP-Binding Proteins