Continuous production of neo-fructooligosaccharides by immobilization of whole cells of Penicillium citrinum.
To produce neo-fructooligosaccharides (neo-FOSs) in a 500 ml continuous packed-bed reactor using whole cell immobilization of Penicillium citrinum KCCM 11663, the optimum reaction conditions were 50 degrees C, pH 6 with 600 g sucrose l-1 being fed as substrate at 1.3 ml min-1 . Under these conditions, the maximum neo-FOSs production was 49 g l-1. In a packed-bed reactor, continuous production of neo-FOSs was possible for 50 d indicating a potential for industrial production.
Park
Man Chul
MC
Department of Chemical and Biological Engineering, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul, 136-701, Korea.
Lim
Jung Soo
JS
Kim
Jin Chul
JC
Park
Seung Won
SW
Kim
Seung Wook
SW
D019149:Bioreactors; D018914:Cells, Immobilized; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D009844:Oligosaccharides / Q000096:biosynthesis*; D010407:Penicillium / Q000166:cytology* / Q000378:metabolism*
D009844:Oligosaccharides; C116580:fructooligosaccharide