Investigating interaction of ligands with voltage dependent anion channel through noise analyses.
Ion channel-protein complexes inserted in the membrane act as molecular gates for transport across the membrane. The opening and closing of these gates can be controlled by one or more variables like ligands (small molecules, proteins, etc.), transmembrane voltage, and the concentration gradient of a chemical across the membrane. We have shown how current noise profile of voltage dependent anion channel can be used to monitor change in the gating of the channel after its modulation by various ligands. This is being proposed as a novel method to probe the interaction of ion channels with ligands.
Banerjee
Jyotirmoy
J
Department of Biophysics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
Ghosh
Subhendu
S
D000818:Animals; D001921:Brain / Q000502:physiology; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry; D008564:Membrane Potentials / Q000502:physiology*; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D010766:Phosphorylation / Q000187:drug effects; D010958:Plasminogen / Q000633:toxicity; D018272:Porins / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000633:toxicity; D051381:Rats; D050994:Voltage-Dependent Anion Channels; D051028:bcl-2-Associated X Protein
D008024:Ligands; D008051:Lipid Bilayers; D018272:Porins; D019253:Proto-Oncogene Proteins c-bcl-2; D050994:Voltage-Dependent Anion Channels; D051028:bcl-2-Associated X Protein; D010958:Plasminogen