title: Sequence analysis of the Escherichia coli O15 antigen gene cluster and development of a PCR assay for rapid detection of intestinal and extraintestinal pathogenic E. coli O15 strains.
abstract: A collection of 33 Escherichia coli serogroup O15 strains was studied with regard to O:H serotypes and virulence markers and for detection of the O-antigen-specific genes wzx and wzy. The strains were from nine different countries, originated from healthy or diseased humans and animals and from food, and were isolated between 1941 and 2003. On the basis of virulence markers and clinical data the strains could be split into different pathogroups, such as uropathogenic E. coli, enteropathogenic E. coli, Shiga toxin-producing E. coli, and enteroaggregative E. coli. H serotyping and genotyping of the flagellin (fliC) gene revealed 11 different H types and a close association between certain H types, virulence markers, and pathogroups was found. Nucleotide sequence analysis of the O-antigen gene cluster revealed putative genes for biosynthesis of O15 antigen. PCR assays were developed for sensitive and specific detection of the O15-antigen-specific genes wzx and wzy. The high pathotype diversity found in the collection of 33 O15 strains contrasted with the high level of similarity found in the genes specific to the O15 antigen. This might indicate that the O15 determinant has been spread by horizontal gene transfer to a number of genetically unrelated strains of E. coli.
authors.affiliation: Division of Microbial Toxins, Robert Koch Institute, Berlin, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.forename: Jiang
authors.initials: J
authors.lastname: Tao
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
authors.forename: Gladys
authors.initials: G
authors.lastname: Krause
authors.forename: Sonja
authors.initials: S
authors.lastname: Zimmermann
authors.forename: Kerstin
authors.initials: K
authors.lastname: Gleier
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Xia
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D006801:Humans; D007410:Intestinal Diseases / Q000175:diagnosis / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013997:Time Factors; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; D019081:O Antigens