title: Differential localization of rat Eag1 and Eag2 K+ channels in hippocampal neurons.
abstract: Two isoforms of rat ether-a-go-go (Eag) K channels, rEag1 and rEag2, are widely expressed in many regions of the brain. The neurophysiological roles of these channels, however, are unclear. We addressed this issue by studying their subcellular localizations in hippocampal neurons. Immunofluorescence studies using markers for different compartments of neurons demonstrated a differential expression pattern of rEag1 and rEag2 K channels in the somatodendritic region. Furthermore, rEag1 K channels were in close proximity to synaptophysin and densin-180, but not GAD65. Our data suggest that both rEag1 and rEag2 K channels may play a pivotal role in the regulation of the excitability of dendrites and somas, and that rEag1 K channels may modulate the postsynaptic signaling of glutamatergic synapses.
authors.affiliation: School of Medicine, Fu Jen Catholic University, Taipei County, Taiwan.
authors.forename: Chung-Jiuan
authors.initials: CJ
authors.lastname: Jeng
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Chang
authors.forename: Chih-Yung
authors.initials: CY
authors.lastname: Tang
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D003712:Dendrites / Q000378:metabolism; D051638:Ether-A-Go-Go Potassium Channels; D005786:Gene Expression Regulation / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D051381:Rats; D012795:Sialoglycoproteins / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013569:Synapses / Q000378:metabolism; D016708:Synaptophysin / Q000378:metabolism; D014162:Transfection / Q000379:methods; D016875:tau Proteins / Q000378:metabolism
chemical_list: D051638:Ether-A-Go-Go Potassium Channels; D007527:Isoenzymes; C496886:Kcnh1 protein, rat; C402401:Kcnh5 protein, rat; C504461:LRRC7 protein, human; C504463:Lrrc7 protein, rat; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012795:Sialoglycoproteins; D016708:Synaptophysin; D016875:tau Proteins; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2