title: Simple determination of riluzole in rat brain by high-performance liquid chromatography and spectrophotometric detection.
abstract: A simple method was developed for separation and quantification of riluzole in rat brain. The analyses were performed by high-performance liquid chromatography using a C18 reversed-phase column (Hypersil ODS) with UV detection at 264 nm. The mobile phase consisted of methanol-water containing 1% triethylamine adjusted with orthophosphoric acid to pH 3.2. The retention time was 8.6 min. A simple liquid-liquid extraction with ethyl acetate was used to obtain riluzole from brain samples. The limit of quantification was 10 ng/g. The recovery was about 80%. The relationship between peak areas and concentrations was linear over the range between 0.01 and 0.8 microg/g, with r2 value over 0.99. The assay provided good reproducibility and accuracy and proved to be suitable for pharmacokinetic studies of riluzole.
authors.affiliation: Department of Experimental and Clinical Pharmacology, School of Medicine, University of Catania, Viale A. Doria 6, Building 2-ex 12, I-95125 Catania, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Maltese
authors.forename: Francesco
authors.initials: F
authors.lastname: Maugeri
authors.forename: Filippo
authors.initials: F
authors.lastname: Drago
authors.forename: Claudio
authors.initials: C
authors.lastname: Bucolo
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018691:Excitatory Amino Acid Antagonists / Q000032:analysis*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019782:Riluzole / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D018691:Excitatory Amino Acid Antagonists; D019782:Riluzole