Alpha-subunit positions methionine 180 and glutamate 214 of Pseudomonas stutzeri OX1 toluene-o-xylene monooxygenase influence catalysis.
Alpha-subunit position M180 of toluene-o-xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o-cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p-nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.
Vardar
Gönül
G
Department of Chemical Engineering, University of Connecticut, Storrs, CT 06269-3222, USA.
Wood
Thomas K
TK
D019943:Amino Acid Substitution; D000596:Amino Acids / Q000235:genetics / Q000502:physiology*; D003408:Cresols / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D044223:Pseudomonas stutzeri / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
D000596:Amino Acids; D003408:Cresols; D021122:Protein Subunits; D010105:Oxygenases; C114360:toluene 2-xylene monooxygenase; C034047:2-cresol