[Studies on tissue culture of Dendrobium lituiflorum].
OBJECTIVE
To select optimal media and conditions for the tissue culture of Dendrobium lituiforum.


METHODS
The basic media, light illumination condition, phytohormone, and natural aqueous extracts. were tested and compared. The chlorophyll contents and soluble protein contents were measured.


RESULTS
N6 medium was suitable for embryo germination and growth. 1/2 MS + NAA 0.5 mg x L(-1) cordd be used as the optimal protocorm multiplying media. Culture 20 days in darkness in advance, and then in light is useful to embryo germinate. 1/2 MS+ NAA 0-0.5 mg x L(-1) is beneficial to protocorm multiplication largely. N6 + 6-BA 2.0 mg x L(-1) + NAA 0.5 mg x L(-1) + 10% potato juice is useful to protocorm differentiation. Phytohormone and potato juice added to the media promoted chlorophyll content and souble protein content. N6 + NAA 0.5 mg x L(-1) + 10% banana juice is the best medium for plantlet rootage and strengthening.


CONCLUSIONS
Concentration of inorganic salt, nitrogen source and illumination condition are important to embryo germination and growth. The nitrogen source type has effect on the protocorm multiplication. The chlorophyll contents and souble protein contents may be index to indicate the growth condition of plantlets, which can help to select the optimal media.
Chang
Jun
J
School of Life Science, Nanjing University, Nanjing Normal 210097, China.
Ding
Xiao-yu
XY
Bao
Shu-ling
SL
Liu
Dong-yang
DY
He
Jia
J
Tang
Feng
F
Ding
Bing-zhong
BZ
D002734:Chlorophyll / Q000032:analysis*; D003470:Culture Media; D031667:Dendrobium / Q000737:chemistry / Q000254:growth & development*; D008027:Light; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development*; D046509:Tissue Culture Techniques
D003470:Culture Media; D010937:Plant Growth Regulators; D010940:Plant Proteins; D002734:Chlorophyll