Potentiation of a dendritic cell vaccine for murine renal cell carcinoma by CpG oligonucleotides.
OBJECTIVE
An ideal vaccine therapy for tumors should activate both effector and memory immune responses against tumor-specific antigens. Here we investigated the effect of CpG oligodeoxynucleotides (CpG-ODN) for their ability to potentiate the activity of tumor antigen-pulsed bone marrow-derived dendritic cells (DC) in a vaccine model for the treatment of murine renal cell carcinoma (RENCA).


METHODS
First we evaluated the effects of a murine renal cell carcinoma (RENCA) on immune cell activity in a mouse model using in vitro assays for T-cell proliferation and natural killer cell activation. To overcome the immune suppression of the tumor, we s.c. injected groups of 10 mice with dendritic cells and tumor cells. We compared the effect of different conditioning regimens of the DCs with RENCA antigen and/or CpG-ODNs before injection by measuring tumor size twice a week.


RESULTS
Tumor growth was shown to negatively affect spleen cell and T-cell proliferation, IFN-gamma production, natural killer cell activity, and NF-kappaB activation in T cells. In this model, we have shown that RENCA-pulsed CpG-ODN-treated DCs were able not only to significantly reduce tumor growth but also to prevent tumor implantation in 60% of mice. Tumor-free mice were resistant to tumor challenge and the immunity conferred by the vaccine was transferable and tumor specific.


CONCLUSIONS
This data show that RENCA down-modulates the immune response, and DC vaccine therapy, in conjunction with CpG-ODN, can restore tumor-specific immunity.
Chagnon
Fanny
F
Department of Surgery (Urology), McGill University, 1650 Cedar Avenue, Montreal, Quebec H3G 1A4, Canada.
Tanguay
Simon
S
Ozdal
Ozdem Levent
OL
Guan
Meng
M
Ozen
Zeynep Z
ZZ
Ripeau
Jean-Sébastien
JS
Chevrette
Mario
M
Elhilali
Mostafa M
MM
Thompson-Snipes
Lu Ann
LA
D000276:Adjuvants, Immunologic / Q000494:pharmacology / Q000627:therapeutic use; D000704:Analysis of Variance; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D016896:Treatment Outcome
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides