Analysis of isoflavone daidzein in Puerariae radix with micelle-mediated extraction and preconcentration.
Nonionic surfactant oligo(ethylene glycol) monoalkyl ether (Genapol X-080) was employed as an alternative and effective solvent for the extraction of daidzein from Puerariae radix for the first time. Optimum experimental conditions were established. With 5% Genapol X-080 (w/v), liquid/solid ratio of 25:1 (mL/g), and ultrasonic-assisted extraction for 45 min, the extraction percentage of daidzein reached the highest value. For the preconcentration of daidzein by cloud-point extraction (CPE), sodium chloride was added to the solution to facilitate the phase separation and increase the preconcentration factor by reducing the volume of the surfactant-rich phase. The preconcentration factor for daidzein was about 13. Satisfactory results were obtained for the analysis of daidzein from P. radix with this established method.
He
Jiantao
J
Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
Zhao
Ziwei
Z
Shi
Zhihong
Z
Zhao
Meiping
M
Li
Yuanzong
Y
Chang
Wenbao
W
D002851:Chromatography, High Pressure Liquid; D007529:Isoflavones / Q000032:analysis*; D008823:Micelles; D010936:Plant Extracts / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*
D007529:Isoflavones; D008823:Micelles; D010936:Plant Extracts; C004742:daidzein