Genomic studies of transcription factor-DNA interactions.
A central issue in the regulation of gene expression is the physical association of transcription factors with relevant promoter sequences. Recently, technological advances have allowed researchers to analyze these processes on a genomic scale. In particular, the combination of the chromatin immunoprecipitation (ChIP) technique with microarray analysis (the 'ChIP to chip' experiment) is providing a wealth of new and surprising data on transcription factor-chromatin interactions. These advances are reviewed here. We also discuss future challenges in the area.
Sikder
Devanjan
D
Department of Internal Medicine, Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390-8573, USA. devanjan.sikder@utsouthwestern.edu
Kodadek
Thomas
T
D000818:Animals; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation; D023281:Genomics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic
D014157:Transcription Factors; D004247:DNA