title: Essential role of an adenylate cyclase in regulating Vibrio vulnificus virulence.
abstract: Vibrio vulnificus, a halophilic estuarine bacterium, causes a fatal septicemia and necrotizing wound infection. To investigate the role of cAMP in V. vulnificus virulence regulation, an in-frame deletion mutant of the cya gene encoding adenylate cyclase was constructed. The cya null mutation resulted in a pleiotropic change of virulence phenotypes. The production of hemolysin and protease, the motility, and the cytotoxicity were decreased by the cya mutation. The defects in the cya mutant were functionally complemented in trans by a plasmid carrying the wild type cya allele. The V. vulnificus cya mutant exhibited a 100-fold increase in LD50 to mice. The result indicates that cAMP plays an essential role in the global regulation of V. vulnificus virulence.
authors.affiliation: Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Chonnam National University Medical School, Gwangju 501-746, South Korea.
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Kim
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Choon Mee
authors.initials: CM
authors.lastname: Kim
authors.forename: Shee Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Joon Haeng
authors.initials: JH
authors.lastname: Rhee
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006460:Hemolysin Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D009154:Mutation; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms; D041261:Vibrio vulnificus / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; D000242:Cyclic AMP; D000262:Adenylyl Cyclases