title: The Shwachman-Bodian-Diamond syndrome protein family is involved in RNA metabolism.
abstract: A combination of structural, biochemical, and genetic studies in model organisms was used to infer a cellular role for the human protein (SBDS) responsible for Shwachman-Bodian-Diamond syndrome. The crystal structure of the SBDS homologue in Archaeoglobus fulgidus, AF0491, revealed a three domain protein. The N-terminal domain, which harbors the majority of disease-linked mutations, has a novel three-dimensional fold. The central domain has the common winged helix-turn-helix motif, and the C-terminal domain shares structural homology with known RNA-binding domains. Proteomic analysis of the SBDS sequence homologue in Saccharomyces cerevisiae, YLR022C, revealed an association with over 20 proteins involved in ribosome biosynthesis. NMR structural genomics revealed another yeast protein, YHR087W, to be a structural homologue of the AF0491 N-terminal domain. Sequence analysis confirmed them as distant sequence homologues, therefore related by divergent evolution. Synthetic genetic array analysis of YHR087W revealed genetic interactions with proteins involved in RNA and rRNA processing including Mdm20/Nat3, Nsr1, and Npl3. Our observations, taken together with previous reports, support the conclusion that SBDS and its homologues play a role in RNA metabolism.
authors.affiliation: Ontario Center for Structural Proteomics, University of Toronto, Canada.
authors.forename: Alexei
authors.initials: A
authors.lastname: Savchenko
authors.forename: Nevan
authors.initials: N
authors.lastname: Krogan
authors.forename: John R
authors.initials: JR
authors.lastname: Cort
authors.forename: Elena
authors.initials: E
authors.lastname: Evdokimova
authors.forename: Jocelyne M
authors.initials: JM
authors.lastname: Lew
authors.forename: Adelinda A
authors.initials: AA
authors.lastname: Yee
authors.forename: Luis
authors.initials: L
authors.lastname: Sánchez-Pulido
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Andrade
authors.forename: Alexey
authors.initials: A
authors.lastname: Bochkarev
authors.forename: James D
authors.initials: JD
authors.lastname: Watson
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kennedy
authors.forename: Jack
authors.initials: J
authors.lastname: Greenblatt
authors.forename: Timothy
authors.initials: T
authors.lastname: Hughes
authors.forename: Cheryl H
authors.initials: CH
authors.lastname: Arrowsmith
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Rommens
authors.forename: Aled M
authors.initials: AM
authors.lastname: Edwards
mesh_terms: D000123:Acetyltransferases; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019611:Archaeoglobus fulgidus / Q000378:metabolism; D018360:Crystallography, X-Ray; D023281:Genomics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D063210:N-Terminal Acetyltransferase B; D009687:Nuclear Proteins / Q000737:chemistry; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism / Q000502:physiology*; D040901:Proteomics / Q000379:methods; D012313:RNA / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity
chemical_list: C105432:MDM20 protein, S cerevisiae; C077734:NPL3 protein, S cerevisiae; C068036:NSR1 protein, S cerevisiae; D009687:Nuclear Proteins; D011506:Proteins; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; C470521:SBDS protein, human; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D000123:Acetyltransferases; D063210:N-Terminal Acetyltransferase B