The contribution of C alpha-H...O hydrogen bonds to membrane protein stability depends on the position of the amide.
Structural analyses of membrane proteins reveal a large number of C(alpha)-H...O contacts between transmembrane helices, presumed to be hydrogen bonds. Recent experiments produced conflicting results for the contribution of such hydrogen bonds to membrane protein stability. An FTIR study estimated an energy of -0.88 kcal/mol for the G79-C(alpha)-H...I76-O hydrogen bond in glycophorin A, whereas a mutagenesis study showed that the A51-C(alpha)-H...T24-O(gamma) hydrogen bond does not stabilize bacteriorhodopsin. Here, we reconcile these results using molecular mechanics calculations and an implicit membrane model (IMM1). With explicit hydrogen atoms, the potential energy of the G79-C(alpha)-H...I76-O interaction in GpA ranges from -0.54 to -0.9 kcal/mol and its contribution to stability (effective energy) from -0.49 to -0.83 kcal/mol, depending on the structural model used. The average values of these quantities in GpA-like motifs are similar. In bR, the corresponding numbers for the A51-C(alpha)-H...T24-O(gamma) interaction are +0.15 and +0.32 kcal/mol. The difference results from the different arrangement of the interacting groups and specifically the position of the acceptor with respect to the C(alpha) and N atoms. This conclusion likely applies to soluble proteins as well.
Mottamal
Madhusoodanan
M
Department of Chemistry, City College of New York, 138th Street and Convent Avenue, New York, New York 10031, USA.
Lazaridis
Themis
T
D000409:Alanine / Q000737:chemistry; D000577:Amides / Q000737:chemistry*; D020816:Amino Acid Motifs; D001436:Bacteriorhodopsins / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D003198:Computer Simulation; D006021:Glycophorins / Q000737:chemistry; D006860:Hydrogen Bonding; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D010100:Oxygen / Q000737:chemistry; D017433:Protein Structure, Secondary; D013816:Thermodynamics; D013912:Threonine / Q000737:chemistry
D000577:Amides; D006021:Glycophorins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D013912:Threonine; D001436:Bacteriorhodopsins; D002244:Carbon; D000409:Alanine; D010100:Oxygen