A fast method for preparation of cytochrome-C of high purity from pig heart by hydrophobic interaction chromatography.
A fast method for the preparation of cytochrome C (Cyt-C) of high purity from pig heart is presented. A simple-type column of hydrophobic interaction chromatography packed with big particles was employed for the fast purification in laboratory scale. The purity of the purified Cyt-C was verified by reversed-phase liquid chromatography, UV spectroscopy, and iron content in it. Compared with the common method, the new method has advantages of higher speed, simpler purification processes, higher mass recoveries and higher purity.
Yao
Cong
C
Institute of Modern Separation Science, Shaanxi Key Laboratory of Modern Separation Science, Northwest University, Xi'an 710069, China.
Ke
Congyu
C
Bai
Quan
Q
Wei
Yinmao
Y
Geng
Xindu
X
D000818:Animals; D002845:Chromatography / Q000379:methods*; D045304:Cytochromes c / Q000302:isolation & purification*; D057927:Hydrophobic and Hydrophilic Interactions; D009206:Myocardium / Q000737:chemistry*; D013552:Swine
D045304:Cytochromes c