Identification and complete sequencing of novel human transcripts through the use of mouse orthologs and testis cDNA sequences.
The correct identification of all human genes, and their derived transcripts, has not yet been achieved, and it remains one of the major aims of the worldwide genomics community. Computational programs suggest the existence of 30,000 to 40,000 human genes. However, definitive gene identification can only be achieved by experimental approaches. We used two distinct methodologies, one based on the alignment of mouse orthologous sequences to the human genome, and another based on the construction of a high-quality human testis cDNA library, in an attempt to identify new human transcripts within the human genome sequence. We generated 47 complete human transcript sequences, comprising 27 unannotated and 20 annotated sequences. Eight of these transcripts are variants of previously known genes. These transcripts were characterized according to size, number of exons, and chromosomal localization, and a search for protein domains was undertaken based on their putative open reading frames. In silico expression analysis suggests that some of these transcripts are expressed at low levels and in a restricted set of tissues.
Ferreira
Elisa N
EN
Laboratory of Molecular Biology and Genomics, Ludwig Institute for Cancer Research, São Paulo, SP, Brazil.
Pires
Lilian C
LC
Parmigiani
Raphael B
RB
Bettoni
Fabiana
F
Puga
Renato D
RD
Pinheiro
Daniel G
DG
Andrade
Luís Eduardo C
LE
Cruz
Luciana O
LO
Degaki
Theri L
TL
Faria
Milton
M
Festa
Fernanda
F
Giannella-Neto
Daniel
D
Giorgi
Ricardo R
RR
Goldman
Gustavo H
GH
Granja
Fabiana
F
Gruber
Arthur
A
Hackel
Christine
C
Henrique-Silva
Flávio
F
Malnic
Bettina
B
Manzini
Carina V B
CV
Marie
Suely K N
SK
Martinez-Rossi
Nilce M
NM
Oba-Shinjo
Sueli M
SM
Pardini
Maria Ines M C
MI
Rahal
Paula
P
Rainho
Cláudia A
CA
Rogatto
Silvia R
SR
Romano
Camila M
CM
Rodrigues
Vanderlei
V
Sales
Magaly M
MM
Savoldi
Marcela
M
da Silva
Ismael D C G
ID
da Silva
Neusa P
NP
de Souza
Sandro J
SJ
Tajara
Eloiza H
EH
Silva
Wilson A
WA
Simpson
Andrew J G
AJ
Sogayar
Mari C
MC
Camargo
Anamaria A
AA
Carraro
Dirce M
DM
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D013737:Testis / Q000737:chemistry*; D014158:Transcription, Genetic / Q000235:genetics*
D018076:DNA, Complementary