Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells.
Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/mlx24 h) reduced c-fos activation by S1P (100 microM-187+/-6% vs. 411+/-27%) and lysophosphatidic acid (LPA; 100 microM-90+/-34% vs. 188+/-41%), but not by sphingosylphosphorylcholine (SPC; 100 microM-390+/-47% vs. 420+/-44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.
Segura
Bradley J
BJ
Department of Surgery, University of Michigan Medical Center, 2101 Taubman Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109-0331, USA.
Zhang
Weizhen
W
Xiao
Liqun
L
Logsdon
Craig D
CD
Mulholland
Michael W
MW
D000818:Animals; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004963:Estrenes / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000502:physiology*; D005910:Glioma; D008246:Lysophospholipids / Q000494:pharmacology*; D051379:Mice; D037342:Pertussis Toxin / Q000494:pharmacology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013997:Time Factors
D004963:Estrenes; D008246:Lysophospholipids; D010726:Phosphodiesterase Inhibitors; D011760:Pyrrolidinones; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; C060506:sphingosine 1-phosphate; D037342:Pertussis Toxin; D013110:Sphingosine; C032881:lysophosphatidic acid