Hypermethylation in promoter region of retinoic acid receptor-beta gene and immunohistochemical findings on retinoic acid receptors in carcinogenesis of endometrium.
In the present study, we analyzed the immunohistochemical findings for the RA receptor (RAR), retinoic X receptor (RXR) and hypermethylation of promoter-region CpG island methylation of RAR-beta2. Immunohistochemistry indicated that though RXR-alpha and -gamma were present in endometrial hyperplasia and cancer, other retinoid receptors were only weakly detected. The hypermethylation of RAR-beta2 was found in 75.0% of endometrial hyperplasia samples and 92.2% of carcinomas. No normal endometria had methylation. This evidence may point to one of the reasons why endometrial hyperplasia acquires high proliferative capacity without differentiation, and the hypermethylation of RAR-beta2 may occur in the early stage of endometrial carcinogenesis.
Li
Rong
R
Department of Obstetrics and Gynecology, School of Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060-8543, Japan.
Saito
Tsuyoshi
T
Tanaka
Ryoichi
R
Satohisa
Seiro
S
Adachi
Katsuya
K
Horie
Miyabi
M
Akashi
Yushi
Y
Kudo
Ryuichi
R
D015153:Blotting, Western; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D004714:Endometrial Hyperplasia / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics*; D047628:Estrogen Receptor alpha / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D011401:Promoter Regions, Genetic; D011980:Receptors, Progesterone / Q000096:biosynthesis; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D047490:Retinoid X Receptor alpha / Q000096:biosynthesis*
D047628:Estrogen Receptor alpha; D011980:Receptors, Progesterone; D018168:Receptors, Retinoic Acid; D047490:Retinoid X Receptor alpha; C082925:retinoic acid receptor beta