Rhodotorula oryzae sp. nov., a novel basidiomycetous yeast species isolated from paddy rice.
A basidiomycetous yeast strain RO-203, which formed orange-red colored colonies, was isolated from a sample of paddy rice crops at the ripe stage in Japan. Morphological, physiological and biochemical characterization indicated that this strain belonged to the genus Rhodotorula. Molecular taxonomic analysis based on the 26S rDNA D1/D2 domain and internal transcribed spacer (ITS) region sequences showed that RO-203 represents an undescribed yeast species, for which the name Rhodotorula oryzae sp. nov. is proposed (type strain: AS 2.2363T = MAFF 516128T). The new species clustered in a branch together with Sakaguchia dacryoidea in phylogenetic trees based on the D1/D2 and ITS sequences. These two species differed by 2.3% and 12% nucleotide divergences in the D1/D2 and ITS regions, respectively.
Bai
Feng-Yan
FY
Systematic Mycology and Lichenology Laboratory, Institure of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China. baify@sun.im.ac.cn
Cai
Yimin
Y
Wang
Qi-Ming
QM
Ohkubo
Hiroto
H
D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D012248:Rhodotorula / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer