title: Influence of various alginate brands on the redifferentiation of dedifferentiated bovine articular chondrocytes in alginate bead culture under high and low oxygen tension.
abstract: We examined the influence of various alginates on the redifferentiation of dedifferentiated articular chondrocytes in alginate bead culture under low (5%) and (21%) high oxygen supply. Isolated bovine articular chondrocytes were dedifferentiated and multiplied by 2-week monolayer culture under 21% oxygen. They were subcultured at a density of 10(7) cells/mL in six different commercially available sodium alginates (1.2%, w/v) and held under 21 or 5% oxygen for 3 weeks. Proliferation (DNA measurement on days 0 and 21 of culture), collagen type II production (immunocytochemistry and Western blotting), and [(3)H]proline and [(35)S]sulfate incorporation were monitored. Collagen type II production was significantly stronger under 5% oxygen compared with 21% oxygen in two alginates (three other alginates nearly reached the significance level). However, alginate-based differences proved not to be significant. [(3)H]Proline incorporation was not influenced by alginate but showed strong oxygen dependency (up to 3-fold higher under 5% oxygen). For [(35)S]sulfate incorporation oxygen dependency was even stronger (up to 8-fold higher under 5% oxygen) and significant alginate-dependent differences were found for several alginates. The effects of the different alginates did not correlate with their pH, viscosity, or guluronic:mannuronic acid ratio. Thus, the type of alginate and even more, the oxygen supply, influence the redifferentiation and matrix production of dedifferentiated bovine articular chondrocytes.
authors.affiliation: Institute of Anatomy, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Domm
authors.forename: M
authors.initials: M
authors.lastname: Schünke
authors.forename: J
authors.initials: J
authors.lastname: Steinhagen
authors.forename: S
authors.initials: S
authors.lastname: Freitag
authors.forename: B
authors.initials: B
authors.lastname: Kurz
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000145:classification; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000502:physiology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D010100:Oxygen