[Transcriptional and electron microscopic analysis of extracellular matrix proteoglycans in acute acetaminophen intoxication].
Acetaminophen (APAP) OTC regimen is not compatible with kidney and liver toxic effects. In order to further understand the determinism of the repair process, we have studied the expression of some target extracellular matrix proteoglycan components in an animal model.


METHODS
An acute APAP toxic dose (800 mg/bw) was administered to 45 mice aged 1 month. At 24 hours, tissue samples were processed for light microscopy, electromicroscopy and RT-PCR analysis.


RESULTS
Extensive necrotic lesions involving hepatocytes and tubular epithelial cells associate strong positivity for proteoglycans, detected as convoluted filaments with lateral projections, frequently associated with collagen fibers, cell surface and other matrix components. Overexpression for perlecan, syndecan 1 and 4 and low expression for biglycan was assessed by RT-PCR.


CONCLUSIONS
Extracellular matrix and cell surface components are early involved in both lesional mechanism and repair reaction post acetaminophen injury.
Cozma
Laurette Graziella
LG
Institutul de Sănătate Publică Iaşi, Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi, Facultatea de Medicină.
Alexa
Ioana Dana
ID
Dobrescu
Gioconda
G
D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D058578:Biglycan; D056486:Chemical and Drug Induced Liver Injury; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans / Q000032:analysis; D008107:Liver Diseases / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D023421:Models, Animal; D011509:Proteoglycans / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans
D018712:Analgesics, Non-Narcotic; C546885:Bgn protein, mouse; D058578:Biglycan; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508242:Sdc1 protein, mouse; C508306:Sdc4 protein, mouse; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans; C071927:perlecan; D000082:Acetaminophen