title: A Rieske ferredoxin typifying a subtype within Rieske proteins: spectroscopic, biochemical and stability studies.
abstract: A new subtype of archaeal Rieske ferredoxin (RFd) has been identified in the genome of the thermoacidophilic archaeon Acidianus ambivalens. The gene is inserted in an atypical genomic context in a gene cluster encoding a NiFe hydrogenase. Sequence and phyletic analysis showed that the protein is related to bacterial RFd but not to any of the known archaeal Rieske proteins. The recombinant 14 kDa protein isolated from Escherichia coli behaved as a dimer in solution. It contained approximately 2 Fe/mol and all visible and EPR spectroscopic features typical of Rieske centre-containing proteins. However, its redox potential (+170 mV) was significantly higher than those of canonical RFd. This difference is rationalized in terms of the protein structure environment, as discrete amino acid substitutions in key positions around the metal centre account for the higher potential.
authors.affiliation: Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany. kletzin@bio.tu-darmstadt.de
authors.forename: Arnulf
authors.initials: A
authors.lastname: Kletzin
authors.forename: Ana S
authors.initials: AS
authors.lastname: Ferreira
authors.forename: Torsten
authors.initials: T
authors.lastname: Hechler
authors.forename: Tiago M
authors.initials: TM
authors.lastname: Bandeiras
authors.forename: Miguel
authors.initials: M
authors.lastname: Teixeira
authors.forename: Cláudio M
authors.initials: CM
authors.lastname: Gomes
mesh_terms: D041541:Acidianus / Q000737:chemistry* / Q000145:classification; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D014450:Electron Transport Complex III / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D005288:Ferredoxins / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D020745:Genome, Archaeal; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008967:Molecular Biology; D010084:Oxidation-Reduction; D010802:Phylogeny; D011487:Protein Conformation; D011489:Protein Denaturation; D016415:Sequence Alignment; D013057:Spectrum Analysis; D013696:Temperature
chemical_list: D019843:Archaeal Proteins; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; D007501:Iron; D014450:Electron Transport Complex III