[Protective effects of acidic fibroblast growth factor on intestinal ischemia/reperfusion in rats].
OBJECTIVE
To observe the change in hepatic and renal functions, change in the plasma D-lactate level, and the expression of proliferating cell nuclear antigen (PCNA) after intestinal I/R injury, so as to explore the effects of reconstructive human acid fibroblast growth factor(aFGF) on intestinal I/R injury in rats.


METHODS
One hundred and twenty-six Wistar rats were divided into sham-operated, ischemia (45 minutes) plus reperfusion, reconstructive human aFGF treatment (2, 4, 8 microg aFGF) and wild type aFGF(2, 6, 12, and 24 hours, respectively) groups. Hepatic and renal functions and the levels of plasma D-lactate were determined and the expression of PCNA was assessed.


RESULTS
Compared with all other groups, bowel barrier function and hepatic and renal functions showed most marked deterioration in sham-operated group. The damages were less marked in reconstructive human aFGF group compared with other groups 24 hours after ischemia/reperfusion of the intestine, and the protective effect was best shown when 4 microg of aFGF was given. The trend of expression of PCNA was similar to that of changes in D-lactate level.


CONCLUSIONS
Wild type reconstructive human aFGF treatment significantly improves the outcome of ischemia/reperfusion injury to the intestine, and the effect is dose-dependent.
Weng
Li-xin
LX
304th Clinical Department of General Hospital of PLA, Beijing 100037, China.
Fu
Xiao-bing
XB
Li
Xiu-xia
XX
Sun
Tong-zhu
TZ
Zheng
Shu-yun
SY
Chen
Wei
W
Li
Jun-you
JY
D000818:Animals; D004195:Disease Models, Animal; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
D018809:Proliferating Cell Nuclear Antigen; D016220:Fibroblast Growth Factor 1; D019344:Lactic Acid