title: [Knockdown of STAT3 expression using siRNA inhibits the growth of prostate cancer cell lines].
abstract: OBJECTIVE
  To study the effects of pSilencer 1.0-U6-siRNA-STAT3 on the growth of PC3 and LNCaP cells.
  
  
  METHODS
  Three pairs of DNA template coding siRNA were synthesized against STAT3 to reconstruct pSilencer 1.0-U6-STAT3-siRNA, which was transfected into PC3 and LNCaP cells. The STAT3 expression in PC3 cells and LNCaP were transfected with pSilencer 1.0-U6-siRNA-STAT3, and it was detected by Western blot and Northern blot. MTT and FCM were used to observe the growth-inhibiting ratio and apoptosis in PC3 cells.
  
  
  RESULTS
  Western blot and Northern blot analyses demonstrated that pSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit the expression of STAT3 in PC3 and LNCaP cells; MIT and FCM results showed that it could suppress the growth of PC3 cells and LNCaP and induce apoptosis of PC3 cells in vitro.
  
  
  CONCLUSIONS
  PSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of PC3 and LNCaP cells and induce the apoptosis of PC3 cells.
authors.affiliation: Department of Pathophysiology, School of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Li-fang
authors.initials: LF
authors.lastname: Gao
authors.forename: De-qi
authors.initials: DQ
authors.lastname: Xu
authors.forename: Yue-ting
authors.initials: YT
authors.lastname: Shao
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Xue-jian
authors.initials: XJ
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor