title: Electrophoretic separations of twelve phenothiazines and N-demethyl derivatives by using capillary zone electrophoresis and micellar electrokinetic chromatography with non ionic surfactant.
abstract: We focused our work on the separation of phenothiazines that are important drugs used for the treatment of psychic diseases. For a better understanding of the metabolism of these solutes, we wanted to separate not only a mixture of 12 phenothiazines but also a mixture containing phenothiazines and their N-demethyl metabolites by capillary electrophoresis. Separations in capillary zone electrophoresis were performed using 3 x 10(-2) mol/L H3PO4 (pH 2.5) but the obtained resolutions were not entirely satisfactory especially with regard to phenothiazine -N-demethyl derivative pairs. To improve the obtained results, we have performed separations by using micellar electrokinetic chromatography. In this approach, we used a running electrolyte containing 3 x 10(-2) mol/L H3PO4 electrolyte (pH 2.5) and octaethylene glycol monododecyl ether (C12E8) as neutral surfactant. By introducing 2 x 10(-3) mol/L C12E8 in the electrolyte, 11 out of 12 phenothiazines have been baseline separated. With respect to the separation of a mixture containing 3 phenothiazines and their 3 demethyl derivatives, we obtained an excellent separation by using a running electrolyte prepared with 7.5 x 10(-4) mol/L C12E8 and 3 x 10(-2) mol/L H3PO4.
authors.affiliation: UMR 6180 Chirotechnologies Catalyse et Biocatalyse, Université Aix-Marseille III, Faculté des Sciences et Techniques St Jérôme, 13397 Marseille Cedex 07, France.
authors.forename: D C
authors.initials: DC
authors.lastname: Le
authors.forename: C J
authors.initials: CJ
authors.lastname: Morin
authors.forename: M
authors.initials: M
authors.lastname: Beljean
authors.forename: A M
authors.initials: AM
authors.lastname: Siouffi
authors.forename: P L
authors.initials: PL
authors.lastname: Desbène
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010640:Phenothiazines / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D010640:Phenothiazines; D013501:Surface-Active Agents