Analysis of gums by capillary electrophoresis with laser induced fluorescence.
Gums were derivatised with the fluorescence reagent, 9-aminopyrene-1,4,6-trisulfonic acid followed by microcentrifuge filtration. The resulting high mass fractions were analysed by capillary electrophoresis (CE) on a polyacrylamide coated capillary with laser induced fluorescence (LIF) detection. A wide pH range of electrolytes was used to study the influences on the electrophoretic mobilities and on the peak shapes of the gums. In this way, the separation of a mixture of five commercial gums, namely iota carrageenan, kappa carrageenan, alginic acid, xanthan and carboxymethyl cellulose (CMC), could be achieved at pH 3.2 with a 25 mmol/L trisodium citrate buffer. It is also shown that a mixture of Arabic gum, Karaya gum and CMC could be separated at pH 7.8 in a similar buffer.
Peng
Jiayu
J
Department of Organic Chemistry, Ghent University, Krijgslaan 281-S4, B-9000 Ghent, Belgium. pengjiayu@hotmail.com
Sandra
Pat
P
D000180:Acrylic Resins / Q000032:analysis* / Q000737:chemistry; D000464:Alginates / Q000032:analysis; D002266:Carboxymethylcellulose Sodium / Q000032:analysis; D002351:Carrageenan / Q000032:analysis; D045424:Complex Mixtures / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D005453:Fluorescence; D005881:Gingiva / Q000737:chemistry*; D020723:Glucuronic Acid / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D007202:Indicators and Reagents; D007834:Lasers; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011721:Pyrenes / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000464:Alginates; D002266:Carboxymethylcellulose Sodium; D002351:Carrageenan; D045424:Complex Mixtures; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007202:Indicators and Reagents; D011135:Polysaccharides, Bacterial; D011721:Pyrenes; D013451:Sulfonic Acids; D000099166:Polyacrylamides; C492698:9-aminopyrene-1,4,6-trisulfonic acid; C002563:xanthan gum