Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography.
Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.
Li
Li
L
ChangChun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Science, ChangChun, PR China.
Tsao
Rong
R
Liu
Zhiqiang
Z
Liu
Shuying
S
Yang
Raymond
R
Young
J Christopher
JC
Zhu
Honghui
H
Deng
Zeyuan
Z
Xie
Mingyong
M
Fu
Zhihong
Z
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification*; D013058:Mass Spectrometry; D010636:Phenols / Q000302:isolation & purification*; D010943:Plantago / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D059808:Polyphenols
D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols