title: Seminested polymerase chain reaction and heteroduplex analysis detects the monoclonality of IgH rearrangement in follicular lymphoma patients with high sensitivity.
abstract: A new method, combining seminested polymerase chain reaction (PCR) with heteroduplex analysis, was utilized to detect follicular lymphoma (FL) cells in peripheral blood. The method, based on the detection of IgH rearrangements in DNA, detected the presence of monoclonal B cells in FL patients with a high frequency.
authors.forename: Amaylia
authors.initials: A
authors.lastname: Oehadian
authors.forename: Naoki
authors.initials: N
authors.lastname: Koide
authors.forename: Mya Mya
authors.initials: MM
authors.lastname: Mu
authors.forename: Ferdaus
authors.initials: F
authors.lastname: Hassan
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokochi
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008224:Lymphoma, Follicular / Q000235:genetics*; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D007143:Immunoglobulin Heavy Chains; D009692:Nucleic Acid Heteroduplexes