title: Two distinctive pathways for recruitment of naive and primed IgM+ B cells to the gut lamina propria.
abstract: Intestinal IgA+ B cells are generated from IgM+ B cells by in situ class switching in two separate gut microenvironments: organized follicular structures and lamina propria (LP). However, the origin of IgM+ B cells in the gut LP is unknown. Transfer experiments to reconstitute IgM+ B cells and IgA plasma cells in LP of aly/aly mice, which are defective in all organized follicular structures because of an NF-kappaB-inducing kinase (NIK) mutation, revealed that naive B cells can directly migrate to the LP. This migration requires NIK-dependent activation of gut stromal cells. By contrast, the entry of gut-primed IgM+ B cells to the LP is independent of stromal cells with functional NIK. Our results indicate that naive B cells directly migrate to the LP by a distinct pathway from gut-primed B cells.
authors.affiliation: RIKEN Research Center for Allergy and Immunology, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Bob
authors.initials: B
authors.lastname: Meek
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Doi
authors.forename: Tasuku
authors.initials: T
authors.lastname: Honjo
authors.forename: Sidonia
authors.initials: S
authors.lastname: Fagarasan
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000276:immunology / Q000502:physiology; D002465:Cell Movement; D004247:DNA / Q000235:genetics; D004064:Digestive System / Q000166:cytology* / Q000276:immunology*; D007075:Immunoglobulin M / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007075:Immunoglobulin M; D004247:DNA