title: Titania-coated monolithic silica as separation medium for high performance liquid chromatography of phosphorus-containing compounds.
abstract: A method of preparing titania-coated monolithic silica stationary phase has been developed to achieve liquid chromatographic separation of phosphorus-containing compounds, which have recently been attracting increasing attention in biochemical research. The titania-coated silica columns exhibited efficient separation with low pressure drop, which is a typical feature of monolithic structures, and also possessed phospho-selectivity, which is a unique property of the titania surface. The material characteristics of titania-coated monolithic silica were examined, and then resin-clad columns were applied to the HPLC analysis of phosphorylated compounds. Highly efficient separation of phosphorylated substances indicated that the novel titania-coated monolithic silica column will find applications as a useful tool in the field of biochemistry, especially in post-genomic analyses.
authors.affiliation: GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama 358-0032, Japan. s-miyazaki@gls.co.jp
authors.forename: Shota
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Muhammed Yusuf
authors.initials: MY
authors.lastname: Miah
authors.forename: Kei
authors.initials: K
authors.lastname: Morisato
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shintani
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kuroha
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakanishi