title: Effect of an oversulfated exopolysaccharide on angiogenesis induced by fibroblast growth factor-2 or vascular endothelial growth factor in vitro.
abstract: The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube formation was related to alpha(6) integrin subunit expression, which was enhanced by 50% in the presence of the growth factors. Indeed, a monoclonal anti-alpha(6) blocking antibody abolished this vascular tube formation. EPS had no effect in any of the experimental conditions, underlying the importance of sulfation in the angiogenic effects of exopolysaccharide. By potentiating the angiogenic activity of FGF-2 and/or VEGF, OS-EPS, which possesses low anticoagulant activity and thus a low hemorrhagic risk, could potentially be used to accelerate vascular wound healing or to promote the growth of collateral blood vessels in ischemic tissues.
authors.affiliation: Institut Nationale de la Santé et de la Recherche Médicale U428, Université Paris V, 75006 Paris, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Matou
authors.forename: Sylvia
authors.initials: S
authors.lastname: Colliec-Jouault
authors.forename: Isabelle
authors.initials: I
authors.lastname: Galy-Fauroux
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Ratiskol
authors.forename: Corinne
authors.initials: C
authors.lastname: Sinquin
authors.forename: Jean
authors.initials: J
authors.lastname: Guezennec
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Fischer
authors.forename: Dominique
authors.initials: D
authors.lastname: Helley
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D042442:Vascular Endothelial Growth Factors / Q000494:pharmacology*
chemical_list: D011135:Polysaccharides, Bacterial; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2