Genetically modified rice seeds accumulating GLP-1 analogue stimulate insulin secretion from a mouse pancreatic beta-cell line.
Glucagon-like peptide-1 (7-36) amide (GLP-1) is the most potent physiological insulinotropic hormone in humans. We produced large amounts of a GLP-1 analogue, [Ser8, Gln26, Asp34]-GLP-1, which is resistant to trypsin-digestion, as part of a chimeric rice seed storage protein, a 26 kDa globulin, in genetically modified rice seeds. Junction sites between GLP-1 analogue and globulin were replaced by tryptic cleavage sites. The highest level of GLP-1 analogue accumulation was approximately 20-50 microg per seed. We found that GLP-1 analogue derived from trypsin-digested genetically modified rice seeds stimulated insulin secretion from a mouse pancreatic beta-cell line, MIN6.
Sugita
Koichi
K
Forestry Science Laboratory, Nippon Paper Industries Co., Ltd., 5-21-1, Oji, Kita-ku, Tokyo 114-0002, Japan.
Endo-Kasahara
Saori
S
Tada
Yoshifumi
Y
Lijun
Yang
Y
Yasuda
Hiroshi
H
Hayashi
Yuji
Y
Jomori
Takahito
T
Ebinuma
Hiroyasu
H
Takaiwa
Fumio
F
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics; D005916:Globulins / Q000235:genetics / Q000378:metabolism; D005934:Glucagon / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D052216:Glucagon-Like Peptide 1; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
D005916:Globulins; D007328:Insulin; D010446:Peptide Fragments; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D052216:Glucagon-Like Peptide 1; D005934:Glucagon