title: [Chlamydia pneumoniae infection in patients with advanced carotid atherosclerosis].
abstract: Despite the growing number of scientific reports showing different markers of infection caused by Chlamydia pneumoniae in patients with advanced atherosclerosis, there is still no clear confirmation of a pathogenetic link between this infection and atherogenesis. The aim of the present study was to investigate the presence C. pneumoniae DNA in peripheral blood mononuclear cells and carotid endarterectomy samples obtained from patients with advanced atherosclerosis according to the presence specific antibodies against C. pneumoniae in serum. The levels of IgG, IgM and IgA antibodies to C. pneumoniae were determined by enzyme immunoassay (EIA) in sera of 36 patients with advanced atherosclerosis undergoing carotid endarterectomy. The presence of C. pneumoniae DNA in the carotid samples and in peripheral blood mononuclear cells was investigated by a nested polymerase chain reaction (PCR). We have not confirm the presence of C. pneumoniae DNA either in the carotid endarterectomy samples or in peripheral blood mononuclear cells, both in patients having the specific antibodies against C. pneumoniae and in seronegative patients. In the studied group of patients with advanced carotid atherosclerosis there was no correlation between the presence of specific antibodies against C. pneumoniae and the presence of C. pneumoniae DNA in endarterectomy samples and peripheral blood mononuclear cells.
authors.affiliation: Narodowy Instytut Zdrowia Publicznego, Warszawa. paolo@cls.edu.pl
authors.forename: Michał
authors.initials: M
authors.lastname: Karyński
authors.forename: Piotr
authors.initials: P
authors.lastname: Słowiński
authors.forename: Paweł
authors.initials: P
authors.lastname: Grzesiowski
authors.forename: Janusz
authors.initials: J
authors.lastname: Fiett
authors.forename: Edyta
authors.initials: E
authors.lastname: Podsiadły
authors.forename: Jarosław
authors.initials: J
authors.lastname: Walory
authors.forename: Stanisława
authors.initials: S
authors.lastname: Tylewska-Wierzbanowska
authors.forename: Walerian
authors.initials: W
authors.lastname: Staszkiewicz
authors.forename: Waleria
authors.initials: W
authors.lastname: Hryniewicz
mesh_terms: D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D002340:Carotid Artery Diseases / Q000382:microbiology*; D023521:Chlamydophila Infections / Q000150:complications*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial