36 kDa glycoprotein isolated from Rhus verniciflua Stokes fruit has a protective activity to glucose/glucose oxidase-induced apoptosis in NIH/3T3 cells.
The present study was carried out to investigate the antioxidative and antiapoptotic activities of 36 kDa RVS glycoprotein isolated from Rhus verniciflua Stokes fruits (RVS) in NIH/3T3 cells in vitro. The results showed that the RVS glycoprotein has scavenging activities of free radicals and hydroxyl radicals, and protects from glucose/glucose oxidase (G/GO)-induced cellular damage in NIH/3T3 cells dose-dependently. For example, cell viability was 43.5% in the G/GO treatment alone, whereas it was 93.8% in the co-treatment with RVS glycoprotein (200 microg/ml). We also demonstrated that RVS glycoprotein inhibits activities of the nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) induced by G/GO, and prevents from G/GO-induced apoptosis in the NIH/3T3 cells. In this regard, the results in this study indicated that RVS glycoprotein has a strong antioxidative activity and an antiapoptotic effect through the modulation activities of NF-kappaB and AP-1in NIH/3T3 cells.
Ko
Jeong-Hyeon
JH
Molecular Biochemistry Laboratory and Biodefensive Substances Group, Institute of Biotechnology, Chonnam National University, Kwangju, #521, 300 Yongbong-Dong 500-757, South Korea.
Lee
Sei-Jung
SJ
Lim
Kye-Taek
KT
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D005947:Glucose / Q000494:pharmacology*; D005949:Glucose Oxidase / Q000494:pharmacology*; D008562:Membrane Glycoproteins; D051379:Mice; D009077:Mucins / Q000494:pharmacology*; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D041681:NIH 3T3 Cells; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology; D029045:Rhus / Q000737:chemistry*; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors; D000098853:Podoplanin
D000975:Antioxidants; D004338:Drug Combinations; D005947:Glucose; D005949:Glucose Oxidase; D008562:Membrane Glycoproteins; D009077:Mucins; D016328:NF-kappa B; D010940:Plant Proteins; D018808:Transcription Factor AP-1; C117468:PDPN protein, human; D000098853:Podoplanin