title: Identification and complete sequencing of novel human transcripts through the use of mouse orthologs and testis cDNA sequences.
abstract: The correct identification of all human genes, and their derived transcripts, has not yet been achieved, and it remains one of the major aims of the worldwide genomics community. Computational programs suggest the existence of 30,000 to 40,000 human genes. However, definitive gene identification can only be achieved by experimental approaches. We used two distinct methodologies, one based on the alignment of mouse orthologous sequences to the human genome, and another based on the construction of a high-quality human testis cDNA library, in an attempt to identify new human transcripts within the human genome sequence. We generated 47 complete human transcript sequences, comprising 27 unannotated and 20 annotated sequences. Eight of these transcripts are variants of previously known genes. These transcripts were characterized according to size, number of exons, and chromosomal localization, and a search for protein domains was undertaken based on their putative open reading frames. In silico expression analysis suggests that some of these transcripts are expressed at low levels and in a restricted set of tissues.
authors.affiliation: Laboratory of Molecular Biology and Genomics, Ludwig Institute for Cancer Research, São Paulo, SP, Brazil.
authors.forename: Elisa N
authors.initials: EN
authors.lastname: Ferreira
authors.forename: Lilian C
authors.initials: LC
authors.lastname: Pires
authors.forename: Raphael B
authors.initials: RB
authors.lastname: Parmigiani
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bettoni
authors.forename: Renato D
authors.initials: RD
authors.lastname: Puga
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Pinheiro
authors.forename: Luís Eduardo C
authors.initials: LE
authors.lastname: Andrade
authors.forename: Luciana O
authors.initials: LO
authors.lastname: Cruz
authors.forename: Theri L
authors.initials: TL
authors.lastname: Degaki
authors.forename: Milton
authors.initials: M
authors.lastname: Faria
authors.forename: Fernanda
authors.initials: F
authors.lastname: Festa
authors.forename: Daniel
authors.initials: D
authors.lastname: Giannella-Neto
authors.forename: Ricardo R
authors.initials: RR
authors.lastname: Giorgi
authors.forename: Gustavo H
authors.initials: GH
authors.lastname: Goldman
authors.forename: Fabiana
authors.initials: F
authors.lastname: Granja
authors.forename: Arthur
authors.initials: A
authors.lastname: Gruber
authors.forename: Christine
authors.initials: C
authors.lastname: Hackel
authors.forename: Flávio
authors.initials: F
authors.lastname: Henrique-Silva
authors.forename: Bettina
authors.initials: B
authors.lastname: Malnic
authors.forename: Carina V B
authors.initials: CV
authors.lastname: Manzini
authors.forename: Suely K N
authors.initials: SK
authors.lastname: Marie
authors.forename: Nilce M
authors.initials: NM
authors.lastname: Martinez-Rossi
authors.forename: Sueli M
authors.initials: SM
authors.lastname: Oba-Shinjo
authors.forename: Maria Ines M C
authors.initials: MI
authors.lastname: Pardini
authors.forename: Paula
authors.initials: P
authors.lastname: Rahal
authors.forename: Cláudia A
authors.initials: CA
authors.lastname: Rainho
authors.forename: Silvia R
authors.initials: SR
authors.lastname: Rogatto
authors.forename: Camila M
authors.initials: CM
authors.lastname: Romano
authors.forename: Vanderlei
authors.initials: V
authors.lastname: Rodrigues
authors.forename: Magaly M
authors.initials: MM
authors.lastname: Sales
authors.forename: Marcela
authors.initials: M
authors.lastname: Savoldi
authors.forename: Ismael D C G
authors.initials: ID
authors.lastname: da Silva
authors.forename: Neusa P
authors.initials: NP
authors.lastname: da Silva
authors.forename: Sandro J
authors.initials: SJ
authors.lastname: de Souza
authors.forename: Eloiza H
authors.initials: EH
authors.lastname: Tajara
authors.forename: Wilson A
authors.initials: WA
authors.lastname: Silva
authors.forename: Andrew J G
authors.initials: AJ
authors.lastname: Simpson
authors.forename: Mari C
authors.initials: MC
authors.lastname: Sogayar
authors.forename: Anamaria A
authors.initials: AA
authors.lastname: Camargo
authors.forename: Dirce M
authors.initials: DM
authors.lastname: Carraro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D013737:Testis / Q000737:chemistry*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D018076:DNA, Complementary