Cryopreservation alters antigenicity of allografts in a porcine model of transplant vasculopathy.
The need for arterial grafts in coronary surgery to complement autologous vessels has generated interest in cryopreservation of small diameter allografts. We evaluated functional and histologic changes occurring in cryopreserved allografts 3 months after porcine femoral artery transplants.


METHODS
Twenty recipient and 15 donor pigs included a control group of 16 fresh and 12 cryopreserved nonimplant arteries were used. Fresh (n=5) and cryopreserved (n=5) autografts were implanted to assess cryopreservation effects in the absence of rejection. Fresh allografts with or without treatment with cyclosporine (CsA) (n=6 of 8) and cryopreserved allografts with or without treatment with CsA (n=6 of 10) were performed to study the antigenicity of cryopreserved allografts. Arteries were stained with hematoxylin and eosin, Masson's trichrome, and orcein for morphometric analyses and immunostained to identify endothelial cells, smooth muscle cells, T lymphocytes, and macrophages.


RESULTS
Among nonimplant arteries, cryopreservation reduced alpha-actin expression and increased the luminal area. All implanted autografts were patent. Cryopreserved autografts showed reduced alpha-actin expression and developed intimal hyperplasia compared to fresh autografts. Treatment with CsA improved the patency of fresh allografts from 0% to 83% (P <.01) and of cryopreserved allografts from 40% to 100% (P <.05). Cryopreserved allografts showed substantial intimal hyperplasia, and fresh allografts had more T lymphocyte infiltration in the intimal layer with aneurysmal dilatation.


CONCLUSIONS
Cryopreservation reduces the deposition of inflammatory cells and prevents the thrombosis or aneurysmal lesions observed in fresh allografts. Therefore, cryopreservation modifies the antigenicity of vascular allografts.
Solanes
N
N
Institut de Malalties Cardiovasculars, Institut d'Investigacions Biomèdiques Agustí Pi Sunyer, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain. nsolanes@clinic.ub.es
Rigol
M
M
Castellà
M
M
Khabiri
E
E
Ramírez
J
J
Segalés
J
J
Roqué
M
M
Agustí
E
E
Pérez-Villa
F
F
Roig
E
E
Pomar
J L
JL
Sanz
G
G
Heras
M
M
D000818:Animals; D003331:Coronary Vessels / Q000187:drug effects / Q000276:immunology / Q000637:transplantation*; D015925:Cryopreservation / Q000379:methods*; D016572:Cyclosporine / Q000494:pharmacology; D006098:Granulocytes / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D023421:Models, Animal; D013552:Swine; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous / Q000276:immunology*; D017539:Tunica Intima / Q000276:immunology; D017540:Tunica Media / Q000276:immunology
D007166:Immunosuppressive Agents; D007519:Isoantigens; D016572:Cyclosporine