Optimization of alkaline protease production by Bacillus sp. using Taguchi methodology.
Optimization of alkaline protease production parameters by Bacillus sp. was investigated using Taguchi methodology. The pH of the medium was observed to be the most significant factor among all selected optimization parameters at an individual level. The combinatorial influence of least significant factors, inoculum level and salt solution concentration (at the individual level), resulted in an interacting severity index of 76%, suggesting their interactive role in the regulation of protease production in this microbial species. Protease production could be improved more than 100% with Taguchi's optimized conditions of the medium composition by this microorganism.
Prakasham
R S
RS
Biochemical and Environmental Engineering Centre, Indian Institute of Chemical Technology, Hyderabad 500 007, India. prakasam@iict.res.in
Rao
Ch Subba
ChS
Rao
R Sreenivas
RS
Rajesham
S
S
Sarma
P N
PN
D000704:Analysis of Variance; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D003470:Culture Media / Q000378:metabolism; D010450:Endopeptidases / Q000096:biosynthesis*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010461:Peptones / Q000378:metabolism; D012492:Salts / Q000378:metabolism; D013045:Species Specificity
D001426:Bacterial Proteins; D003470:Culture Media; D010461:Peptones; D012492:Salts; D010450:Endopeptidases; C087822:alkaline protease; D005947:Glucose