Plasma thrombin-activatable fibrinolytic inhibitor (TAFI) among healthy subjects and patients with vascular diseases: a validation study.
Thrombin activable fibrinolysis inhibitor antigen levels (TAFI Ag ) exhibit a great inter-individual variability in healthy populations. Our aim is to determine whether variability is due to physiologic variations depending on genetic control or due to validation of the method,in order to allow a better interpretation of the results inpatients with vascular diseases. With this purpose, we performed a strategy validation of specific ELISA method, Zymutest TAFI Ag Hyphen Biomed, base don a commercial monoclonal antibody. After methodology validation we have recently determined plasma TAFI Ag levels in several groups of diseases such as septic patients, menopause and cerebrovascular diseases. TAFI was finally determined in acute ischemic stroke to know its relationship with stroke evolution and response to thrombolytic treatments.
Monasterio
J
J
Vascular Biology and Haemostasis Research Unit, Universitary Hospital Vall d'Hebron, Barcelona, Spain. 5877jma@comb.es
Bermúdez
P
P
Quiroga
D
D
Francisco
E
E
Meneses
B
B
Montaner
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000925:Anticoagulants / Q000097:blood; D002545:Brain Ischemia / Q000097:blood; D025901:Carboxypeptidase B2 / Q000032:analysis / Q000097:blood* / Q000276:immunology; D002536:Cerebral Arteries / Q000378:metabolism; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D015203:Reproducibility of Results; D020521:Stroke / Q000097:blood; D013927:Thrombosis / Q000097:blood* / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D000925:Anticoagulants; D025901:Carboxypeptidase B2