[Expressions of cyclooxygenase-2 and cholecystokinin-B receptor mRNA in human colonic cancer and their significances].
OBJECTIVE
Some researches suggested that expressions of cyclooxygenase-2 (COX-2) and cholecystokinin-B (CCK-B) receptor were elevated in various gastrointestinal tumors, which contributed to progression of tumor. This study was to detect expressions of COX-2 and CCK-B receptor in human colonic cancer tissue and relevant adjacent noncancerous mucosa, explore their significances in tumorigenesis and development of colorectal cancer.


METHODS
Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect expressions of COX-2 and CCK-B receptor in 35 specimens of colonic cancer tissues and relevant adjacent noncancerous mucosa.


RESULTS
mRNA level of COX-2 in colonic cancer tissue was significantly higher than that in normal mucosa (1.3+/-0.3 vs. 0.5+/-0.1, P < 0.05), it was positively related with lymph node metastasis, while not related with other clinicopathologic parameters, such as age, gender, differentiation degree, depth of invasion, and clinical stage (P > 0.05). mRNA level of CCK-B receptor in colonic cancer tissue was significantly higher than that in normal mucosa (0.7+/-0.1 vs. 0.2+/-0.1, P < 0.05).


CONCLUSIONS
Expressions of COX-2 and CCK-B receptor were up-regulated in colonic cancer tissue.
Liu
Zhu-Jun
ZJ
Department of Gastroenterology, People's Hospital, Wuhan University, Wuhan, Hubei, 430061, P. R. China. lzj19761125@126.com
Yu
Jie-Ping
JP
Yu
Hong-Gang
HG
Zhang
Xian-Feng
XF
Luo
He-Sheng
HS
D000328:Adult; D000368:Aged; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043887:Receptor, Cholecystokinin B / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D012333:RNA, Messenger; D043887:Receptor, Cholecystokinin B; D051546:Cyclooxygenase 2