Culture methods differ on the isolation of Salmonella enterica serotypes from naturally contaminated swine fecal samples.
Four culture methods (A, B, C, and D) were comparatively evaluated for their ability to isolate Salmonella enterica from pooled swine fecal samples (n = 100). None of the methods was able to isolate Salmonella from all positive samples. The relative sensitivity of the culture methods evaluated was 82%, 94%, 95%, and 78% for methods A, B, C, and D, respectively. The comparison of sensitivities showed that methods B and C performed significantly better (P < 0.05) than methods A and D. Although relative sensitivities of methods B and C were equal, from the 89 positive samples concomitantly detected by both, 35 (39.3%) had different serotypes (no match) isolated by each method. On the basis of the results of this study, it was concluded that culture methods differ on the isolation of S. enterica serotypes from naturally contaminated swine fecal samples. Depending on the objective(s) of investigations on the ecology and epidemiology of S. enterica in swine populations, a method or a combination of methods should be considered for more reliable results.
Rostagno
Marcos H
MH
National Animal Disease Center, USDA, Ames, IA 50010, USA.
Gailey
Jared K
JK
Hurd
H Scott
HS
Mckean
James D
JD
Leite
Romulo C
RC
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary*; D046508:Culture Techniques / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D019779:Salmonella enterica / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*