Inheritance of resistance and cross-resistance to deltamethrin in Plutella xylostella (Lepidoptera: Plutellidae) from Pakistan.
A field population of Plutella xylostella (L) from Pakistan was found to be highly resistant to deltamethrin (>500-fold) but had little or no resistance to spinosad, fipronil, indoxacarb, abamectin, Bacillus thuringiensis (Bt) var kurstaki, Bt var aizawai or Cry1Ac when compared with a susceptible laboratory population, Lab-UK. A sub-population was selected for six generations (laboratory G3-G8) with deltamethrin (delta-SEL), while a second sub-population was left unselected (UNSEL). Bioassays at G9 found that selection with deltamethrin gave resistance ratios of >230 compared with UNSEL (>6730 compared with Lab-UK). The delta-selected population showed no apparent cross-resistance to spinosad, fipronil or indoxacarb. Logit regression analysis of F1 reciprocal crosses between delta-SEL and Lab-UK indicated that resistance to deltamethrin in the delta-SEL population was inherited as an autosomal, incompletely dominant (D(LC) = 0.67) trait. A direct test of monogenic inheritance based on a back-cross of F1 progeny with delta-SEL suggested that resistance to deltamethrin was controlled by more than one factor.
Sayyed
Ali H
AH
Department of Biological Sciences, Imperial College London, Silwood Park campus, Ascot, Berkshire, SL5 7PY, UK. h.sayyed@imperial.ac.uk
Attique
M Naveed R
MN
Khaliq
Abdul
A
Wright
Denis J
DJ
D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004338:Drug Combinations; D004731:Endotoxins; D006460:Hemolysin Proteins; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides; D007559:Ivermectin / Q000031:analogs & derivatives; D007915:Lepidoptera / Q000235:genetics*; D007928:Lethal Dose 50; D018942:Macrolides; D009570:Nitriles; D010078:Oxazines; D010154:Pakistan; D011720:Pyrazoles; D011722:Pyrethrins
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004338:Drug Combinations; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D018942:Macrolides; D009570:Nitriles; D010078:Oxazines; D011720:Pyrazoles; D011722:Pyrethrins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C017180:decamethrin; C401104:indoxacarb; C048324:abamectin; D007559:Ivermectin; C082360:fipronil; C415329:spinosad