title: [Determination of sodium monofluoroacetate in human blood and food samples by ion chromatography].
abstract: A method was developed for the determination of sodium monofluoroacetate in human blood by ion chromatography. The analysis was performed on a Dionex Ion Pac AS11 column (250 mm x 4 mm i.d.) with an AG11 guard column (50 mm x 4 mm i.d.). A conductivity detector, an SRS suppressor and a 25 microL sample loop were used. The eluent was 2.0 mmol/L Na2B4O7 with a flow rate of 1.0 mL/min. A blood sample of 0.2 mL was pipetted into a test tube followed by adding 1.0 mL of pure water. And then acetonitrile was used to dilute it to 3.0 mL. After the protein was settled down from the blood sample, the supernatant layer was filtered through a 0.45 microm filter before injected into the ion chromatographic system. Good linear relationship between the peak area and the concentration of sodium monofluoroacetate was found with correlation coefficient (r2) of 0.9978 within the range from 0.10 to 10.0 mg/L. The detection limit for blood was 2.5 mg/L. This method also can be used for the determination of sodium monofluoroacetate in food samples.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Wenbing
authors.initials: W
authors.lastname: Shi
authors.forename: Haiyun
authors.initials: H
authors.lastname: Zhou
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.forename: Qinying
authors.initials: Q
authors.lastname: Deng
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D005463:Fluoroacetates / Q000032:analysis* / Q000097:blood*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D012275:Oryza / Q000737:chemistry; D012378:Rodenticides / Q000032:analysis* / Q000097:blood
chemical_list: D005463:Fluoroacetates; D012378:Rodenticides; C014815:fluoroacetic acid