title: In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level.
abstract: The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A' reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level.
authors.affiliation: Department of Genetics, Microbiology and Toxicology, Stockholm University, S-10691 Stockholm, Sweden.
authors.forename: Victor V
authors.initials: VV
authors.lastname: Croitoru
authors.forename: Margarete
authors.initials: M
authors.lastname: Bucheli-Witschel
authors.forename: Leif A
authors.initials: LA
authors.lastname: Isaksson
mesh_terms: D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D039861:Prokaryotic Initiation Factor-1 / Q000235:genetics* / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D003062:Codon; D039861:Prokaryotic Initiation Factor-1; D012333:RNA, Messenger