Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coli.
Treatment of Escherichia coli with non-lethal doses of heat or benzyl alcohol (BA) causes transient membrane fluidization and permeabilization, and induces the rapid transcription of heat-shock genes in a sigma32-dependent manner. This early response is followed by a rapid adaptation (priming) of the cells to otherwise lethal elevated temperature, in strong correlation with an observed remodeling of the composition and alkyl chain unsaturation of membrane lipids. The acquisition of cellular thermotolerance in BA-primed cells is unrelated to protein denaturation and is not accompanied by the formation of major heat-shock proteins, such as GroEL and DnaK. This suggests that the rapid remodeling of membrane composition is sufficient for the short-term bacterial thermotolerance.
Shigapova
Natalia
N
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary.
Török
Zsolt
Z
Balogh
Gábor
G
Goloubinoff
Pierre
P
Vígh
László
L
Horváth
Ibolya
I
D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D019905:Benzyl Alcohol / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects* / Q000648:ultrastructure; D018869:Heat-Shock Response / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D006358:Hot Temperature; D008560:Membrane Fluidity / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*
D019905:Benzyl Alcohol