[Protective effects of salidroside on injury induced by hypoxia/hypoglycemia in cultured neurons].
OBJECTIVE
To study the protective effects of salidroside on injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cell.


METHODS
Apoptosis and intracellular free calcium concentration ([Ca2+]i) were measured by flow cytometry, morphological changes and neuronal necrosis were observed with fluorescence microscope, and the lactic dehydrogenate (LDH) release was measured by LDH kits.


RESULTS
Hypoxia/hypoglycemia cultures for 2 hours induced neuronal apoptosis and necrosis. They were 18.59% (P < 0.01) and 4.94% (P < 0.01) respectively. There were morphological changes including chromatin condensation, nuclear fragmentation and formed apoptotic bodies after exposed to hypoxia/hypoglycemia for 2, 4, 6, 12 hours. After 2 hours of hypoxia/hypoglycemia, neuronal [Ca2+]i and the release of LDH were significantly increased. They were 8.46 nmol/L (P < 0.01) and 16.59% (P < 0.01) respectively. The effects were enhanced with the extending time of hypoxia/hypoglycemia. Salidroside might have significantly decreased the percentage of neuronal apoptosis and necrosis, reduced neuronal [Ca2+]i and the release of LDH. The effects of salidroside were strengthened with the increasing of Salidroside dosage.


CONCLUSIONS
Salidroside has effect of anti-neuronal apoptosis. This effect might be related to its function of decreasing intracellular free calcium concentration.
Zhang
Wen-sheng
WS
College of Resources Science & Technology, Beijing Normal University, Beijing 100875, China. zws@bnu.edu.cn
Zhu
Ling-qun
LQ
Niu
Fu-ling
FL
Deng
Rui-chun
RC
Ma
Chuan-xiang
CX
D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia / Q000187:drug effects; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007003:Hypoglycemia / Q000378:metabolism / Q000473:pathology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D029771:Rhodiola / Q000737:chemistry
D005960:Glucosides; D018696:Neuroprotective Agents; D010636:Phenols; D007770:L-Lactate Dehydrogenase; C009172:rhodioloside; D002118:Calcium