Processing of DNA for nonhomologous end-joining by cell-free extract.
In mammalian cells, nonhomologous end-joining (NHEJ) repairs DNA double-strand breaks created by ionizing radiation and V(D)J recombination. We have developed a cell-free system capable of processing and joining noncompatible DNA ends. The system had key features of NHEJ in vivo, including dependence on Ku, DNA-PKcs, and XRCC4/Ligase4. The NHEJ reaction had striking properties. Processing of noncompatible ends involved polymerase and nuclease activities that often stabilized the alignment of opposing ends by base pairing. To achieve this, polymerase activity efficiently synthesized DNA across discontinuities in the template strand, and nuclease activity removed a limited number of nucleotides back to regions of microhomology. Processing was suppressed for DNA ends that could be ligated directly, biasing the reaction to preserve DNA sequence and maintain genomic integrity. DNA sequence internal to the ends influenced the spectrum of processing events for noncompatible ends. Furthermore, internal DNA sequence strongly influenced joining efficiency, even in the absence of processing. These results support a model in which DNA-PKcs plays a central role in regulating the processing of ends for NHEJ.
Budman
Joe
J
Department of Medicine, Stanford University, Stanford, CA 94305-5151, USA.
Chu
Gilbert
G
D001483:Base Sequence; D002457:Cell Extracts / Q000737:chemistry*; D002460:Cell Line; D002474:Cell-Free System; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics*; D013698:Templates, Genetic
D002457:Cell Extracts; D004247:DNA; D003851:Deoxyribonucleases