Biosynthesis of uridine diphosphate N-acetyl-L-fucosamine in a cell-free system from Salmonella arizonae O:59.
The conversion of uridine diphosphate N-acetyl-D-glucosamine into uridine diphosphate N-acetyl-L-fucosamine was demonstrated with enzymes from cytoplasmic fraction of Salmonella arizonae O:59 cells in the presence of NAD+ (NADP+) and NADPH. The reaction product was identified by ion-pair, reverse-phase HPLC with the use of synthetic nucleoside diphosphate sugar standards under conditions specially developed for separation of uridine diphosphate 2-acetamido-2,6-dideoxyhexoses. L-Fucose dehydrogenase from porcine liver was shown to be applicable for determination of N-acetyl-L-fucosamine, this enzyme being used to confirm L-configuration of the amino sugar residue in the sugar nucleotide formed.
Druzhinina
T N
TN
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, Moscow 119991, Russia.
Kalinchuk
N A
NA
Shibaev
V N
VN
D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D003593:Cytoplasm / Q000201:enzymology; D004590:Electrophoresis, Paper; D012476:Salmonella arizonae / Q000201:enzymology / Q000378:metabolism*; D013552:Swine; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000096:biosynthesis*
C423446:UDP-N-acetylfucosamine; D014539:Uridine Diphosphate Sugars; D014537:Uridine Diphosphate N-Acetylglucosamine; D002237:Carbohydrate Dehydrogenases; C020154:L-fucose dehydrogenase