Pyrosequencing-based approach for rapid detection of rifampin-resistant Mycobacterium tuberculosis.
Rifampin resistance in Mycobacterium tuberculosis could be determined within 2 h by using pyrosequencing-based approach. Pyrosequencing results of rifampin-resistant (n = 21) and rifampin-susceptible (n = 20) M. tuberculosis isolates were concordant with those based on drug susceptibility testing and conventional DNA sequencing. Results showed pyrosequencing-based approach is a rapid, sensitive, and efficient detection method of rifampin-resistant M. tuberculosis.
Zhao
Jin Rong
JR
Institute of Genetic Diagnosis, Fourth Military Medical University, Xi'an 710032, People's Republic of China. zhaojrr@126.com
Bai
Yu Jie
YJ
Zhang
Qing Hua
QH
Wang
Yan
Y
Luo
Ming
M
Yan
Xiao Jun
XJ
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012293:Rifampin / Q000494:pharmacology*; D012494:Sampling Studies; D012680:Sensitivity and Specificity
D000904:Antibiotics, Antitubercular; D004269:DNA, Bacterial; D007202:Indicators and Reagents; D012293:Rifampin