title: TEDOR with adiabatic inversion pulses: Resonance assignments of 13C/15N labelled RNAs.
abstract: We have examined via numerical simulations the performance characteristics of different 15N RF pulse schemes employed in the transferred echo double resonance (TEDOR) experimental protocol for generating 13C-15N dipolar chemical shift correlation spectra of isotopically labelled biological systems at moderate MAS frequencies (omega(r) approximately 10 kHz). With an 15N field strength of approximately 30-35 kHz that is typically available in 5 mm triple resonance MAS NMR probes, it is shown that a robust TEDOR sequence with significant tolerance to experimental imperfections sa as H1 inhomogeneity and resonance offsets can be effectively implemented using adiabatic heteronuclear dipolar recoupling pulse schemes. TEDOR-based 15N-13C and 15N-13C-13C chemical shift correlation experiments were carried out for obtaining 13C and 15N resonance assignments of an RNA composed of 97 (CUG) repeats which has been implicated in the neuromuscular disease myotonic dystrophy.
authors.affiliation: Abteilung Molekulare Biophysik/NMR-Spektroskopie, Institut für Molekulare Biotechnologie, 07745 Jena, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Riedel
authors.forename: Jörg
authors.initials: J
authors.lastname: Leppert
authors.forename: Oliver
authors.initials: O
authors.lastname: Ohlenschläger
authors.forename: Matthias
authors.initials: M
authors.lastname: Görlach
authors.forename: Ramadurai
authors.initials: R
authors.lastname: Ramachandran
mesh_terms: D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes; D012313:RNA / Q000737:chemistry*; D013997:Time Factors
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D012313:RNA