Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach.
Common to numerous differentiation pathways in vertebrate organisms is the regulation of key genes through epigenetic mechanisms. Less well studied is to what extent cells of a given differentiation state, but examined at different points within the life history of an organism, are distinct at the level of the epigenome. A few instances of such variation have been reported, and it would be of considerable value to have at hand a means to characterize additional examples more efficiently. We describe an integrated approach to this task, and further present evidence for regions of age-related histone H4 acetylation change extending over tens to hundreds of kilobases. Broad similarity between two distinct regions of such change suggests a previously unsuspected link between developmental programs and aging.
Russanova
Valya R
VR
National Institute for Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Hirai
Tazuko H
TH
Tchernov
Andrei V
AV
Howard
Bruce H
BH
D000107:Acetylation; D000375:Aging / Q000235:genetics*; D042002:Chromatin Assembly and Disassembly; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002874:Chromosome Mapping; D015894:Genome, Human; D006657:Histones / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction
D006657:Histones