New GABAergic interneurons in the adult neocortex and striatum are generated from different precursors.
Ongoing neurogenesis in the adult mammalian dentate gyrus and olfactory bulb is generally accepted, but its existence in other adult brain regions is highly controversial. We labeled newly born cells in adult rats with the S-phase marker bromodeoxyuridine (BrdU) and used neuronal markers to characterize new cells at different time points after cell division. In the neocortex and striatum, we found BrdU-labeled cells that expressed each of the eight neuronal markers. Their size as well as staining for gamma-aminobutyric acid (GABA), glutamic acid decarboxylase 67, calretinin and/or calbindin, suggest that new neurons in both regions are GABAergic interneurons. BrdU and doublecortin-immunoreactive (BrdU+/DCX+) cells were seen within the striatum, suggesting migration of immature neurons from the subventricular zone. Surprisingly, no DCX+ cells were found within the neocortex. NG2 immunoreactivity in some new neocortical neurons suggested that they may instead be generated from the NG2+ precursors that reside within the cortex itself.
Dayer
Alexandre G
AG
Unit on Neuroplasticity, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA.
Cleaver
Kathryn M
KM
Abouantoun
Thamara
T
Cameron
Heather A
HA
D027322:Amino Acid Transport System X-AG / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000032:analysis; D001973:Bromodeoxyuridine / Q000378:metabolism; D064032:Calbindin 2; D064026:Calbindins; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D003342:Corpus Striatum / Q000737:chemistry / Q000166:cytology*; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D005968:Glutamate Decarboxylase / Q000032:analysis; D050491:Glutamate Plasma Membrane Transport Proteins; D007150:Immunohistochemistry; D007395:Interneurons / Q000737:chemistry / Q000166:cytology*; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000032:analysis; D019579:Neocortex / Q000737:chemistry / Q000166:cytology*; D009419:Nerve Tissue Proteins / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D016601:RNA-Binding Proteins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D027161:Satellite Cells, Perineuronal / Q000737:chemistry / Q000166:cytology; D027981:Symporters / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis*; D000099216:Chondroitin Sulfate Proteoglycan 4
D027322:Amino Acid Transport System X-AG; D000941:Antigens; D001973:Bromodeoxyuridine; D064032:Calbindin 2; D064026:Calbindins; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D005968:Glutamate Decarboxylase; D050491:Glutamate Plasma Membrane Transport Proteins; D007527:Isoenzymes; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011509:Proteoglycans; D016601:RNA-Binding Proteins; D064030:S100 Calcium Binding Protein G; D027981:Symporters; D005680:gamma-Aminobutyric Acid; D000099216:Chondroitin Sulfate Proteoglycan 4; C576764:CALB2 protein, human; C576511:Calb2 protein, mouse; C576512:Calb2 protein, rat; C000714328:DCX protein, human; C000714847:Dcx protein, mouse; C000714848:Dcx protein, rat; C449760:Dpysl3 protein, rat; C490263:Elavl3 protein, mouse; C402565:glutamate decarboxylase 1