title: Correlation of KIT and platelet-derived growth factor receptor alpha mutations with gene activation and expression profiles in gastrointestinal stromal tumors.
abstract: Activating mutations of KIT and platelet-derived growth factor receptor alpha (PDGFRA) are known to be alternative and mutually exclusive genetic events in the development of gastrointestinal stromal tumors (GISTs). We examined the effect of the mutations of these two genes on the gene expression profile of 22 GISTs using the oligonucleotide microarray. Mutations of KIT and PDGFRA were found in 17 cases and three cases, respectively. The remaining two cases had no detectable mutations in either gene. The mutation status of KIT and PDGFRA was directly related to the expression levels of activated KIT and PDGFRA, and was also related to the different expression levels of activated proteins that play key roles in the downstream of the receptor tyrosine kinase III family. To evaluate the impact of mutation status and the importance of the type of mutation in gene expression and clinical features, microarray-derived data from 22 GISTs were interpreted using a principal component analysis (PCA). Three relevant principal component representing mutation of KIT, PDGFRA and chromosome 14q deletion were identified from the interpretation of the oligonucleotide microarray data with PCA. After supervised analysis, there was at least a two fold difference in expression between GISTs with KIT and PDGFRA mutation in 70 genes. Our findings demonstrate that mutations of KIT and PDGFRA affect differential activation and expression of some genes, and can be used for the molecular classification of GISTs.
authors.affiliation: Department of Pathology, 134 Sichon-dong, Seodaemun-gu, CPO Box 8044, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Kang
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Nam
authors.forename: Hyunki
authors.initials: H
authors.lastname: Kim
authors.forename: Hwanseok
authors.initials: H
authors.lastname: Rhee
authors.forename: Nam-Gyun
authors.initials: NG
authors.lastname: Kim
authors.forename: Haeryoung
authors.initials: H
authors.lastname: Kim
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Hyung
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Noh
authors.forename: Joo-Hang
authors.initials: JH
authors.lastname: Kim
authors.forename: Chae-Ok
authors.initials: CO
authors.lastname: Yun
authors.forename: Edison T
authors.initials: ET
authors.lastname: Liu
authors.forename: Hoguen
authors.initials: H
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D004252:DNA Mutational Analysis; D005260:Female; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D025341:Principal Component Analysis; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D015533:Transcriptional Activation
chemical_list: D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D019009:Proto-Oncogene Proteins c-kit