title: Protein-4.2 association with band 3 (AE1, SLCA4) in Xenopus oocytes: effects of three natural protein-4.2 mutations associated with hemolytic anemia.
abstract: We have investigated the effects of coexpression of protein 4.2 and three protein-4.2 variants with band 3 in the Xenopus oocyte expression system. Normal protein 4.2 increased band-3-specific chloride transport in the oocytes. Protein 4.2 also coimmunoprecipitated with band 3 and colocalized with band 3 at the oocyte plasma membrane. The increase in band-3-mediated chloride transport and coimmunoprecipitation of protein 4.2 required the presence of the N-terminal cytoplasmic domain of band 3. Protein 4.2 also localized to the oocyte plasma membrane in the absence of band 3. The protein-4.2 variants 4.2 Tozeur (R310Q) and 4.2 Komatsu (D175Y) had impaired ability to bind to band 3 and these variants did not localize to the oocyte plasma membrane when expressed on their own or when coexpressed with band 3. Unexpectedly, 4.2 Nippon (A142T) behaved similarly to normal protein 4.2. In the absence of a crystal structure of protein 4.2, we propose a homology model of protein 4.2 based on the structure of the sequence-related protein transglutaminase. Using our results in oocytes and this homology model we speculate how these mutations affect protein 4.2 and result in hereditary spherocytosis.
authors.affiliation: Department of Biochemistry, School of Medical Sciences, University of Bristol, BS8 1TD, United Kingdom. ash.m.toye@bristol.ac.uk
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Toye
authors.forename: Sandip
authors.initials: S
authors.lastname: Ghosh
authors.forename: Mark T
authors.initials: MT
authors.lastname: Young
authors.forename: Graham K
authors.initials: GK
authors.lastname: Jones
authors.forename: Richard B
authors.initials: RB
authors.lastname: Sessions
authors.forename: Martine
authors.initials: M
authors.lastname: Ramaugé
authors.forename: Philippe
authors.initials: P
authors.lastname: Leclerc
authors.forename: Joyoti
authors.initials: J
authors.lastname: Basu
authors.forename: Jean
authors.initials: J
authors.lastname: Delaunay
authors.forename: Michael J A
authors.initials: MJ
authors.lastname: Tanner
mesh_terms: D000743:Anemia, Hemolytic / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics / Q000378:metabolism*; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003598:Cytoskeletal Proteins; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins; D018613:Microscopy, Confocal; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014982:Xenopus laevis
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C044791:erythrocyte membrane band 4.2 protein