Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-iota in human glioma cells.
Cyclin-dependent kinase activating kinase (CAK) is a trimeric complex composed of cdk7, cyclin H and MAT1. CAK/cdk7 functions as a master cell cycle regulator by phosphorylating cyclin-dependent kinases for cell cycle progression. We have previously reported that protein kinase C-iota (PKC-iota) associates with CAK/cdk7. In this investigation, immunofluorescence confocal microscopy was used to provide further evidence for the co-localization of PKC-iota with CAK/cdk7. PKC-iota was labeled with Alexa Fluor 488 (green fluorescent dye) and CAK/cdk7 was labeled with Alexa Fluor 555 (red fluorescent dye). The fusion of the red and green fluorescent colors produced a yellow color, which was used to quantify co-localization of PKC-iota and CAK/cdk7. Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG cells.
Bicaku
Elona
E
Department of Chemistry, University of South Florida, USA.
Patel
Rekha
R
Acevedo-Duncan
Mildred
M
D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000201:enzymology*; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D018613:Microscopy, Confocal; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction; D000095043:Cyclin-Dependent Kinase-Activating Kinase; D000099293:Protein Kinase C-lambda
D018844:Cyclin-Dependent Kinases; D007527:Isoenzymes; D011493:Protein Kinase C; D000095043:Cyclin-Dependent Kinase-Activating Kinase; D000099293:Protein Kinase C-lambda; C000722416:CDK7 protein, human