Seminested polymerase chain reaction and heteroduplex analysis detects the monoclonality of IgH rearrangement in follicular lymphoma patients with high sensitivity.
A new method, combining seminested polymerase chain reaction (PCR) with heteroduplex analysis, was utilized to detect follicular lymphoma (FL) cells in peripheral blood. The method, based on the detection of IgH rearrangements in DNA, detected the presence of monoclonal B cells in FL patients with a high frequency.
Oehadian
Amaylia
A
Koide
Naoki
N
Mu
Mya Mya
MM
Hassan
Ferdaus
F
Yoshida
Tomoaki
T
Yokochi
Takashi
T
D001402:B-Lymphocytes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008224:Lymphoma, Follicular / Q000235:genetics*; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D007143:Immunoglobulin Heavy Chains; D009692:Nucleic Acid Heteroduplexes