[Acellular porcine dermal matrix produced with different methods and an experimental study on its transplantation to skin wound].
OBJECTIVE
To observed the effect of healing quality of composite skin grafting consisting of acellular porcine dermal matrix combined with autologous split-thickness skin graft.


METHODS
Porcine skin was treated with dispase II/triton X-100 or hyperosmotic saline/sodium-dodecyl-sulfate (SDS) respectively, and acellular porcine dermal matrix I (APDMI) and APDM II were obtained. Sixty-three Sprague-Dawley rats with full-thickness skin defects on the back were separately covered with APDMT + split-thickness autologous skin, or APDM II + split-thickness autologous skin. The quality of wound healing was observed, the rates of survival and contraction of the grafts were calculated, the tissue samples were harvested for histological examination, and compared with that of autologous split-thickness skin graft.


RESULTS
The wound healing quality of composite skin I, and II was good. There was no significant difference in the rate of survival and contraction of the grafts between the two composite skin grafting groups. It was indicated by histological examination intact basal membrane. There was no significant difference in the survival rate between composite skin grafting groups and autologous split-thickness skin at the 6 th week after operation, but the contraction rates of the grafts in the composite skin groups were lower.


CONCLUSIONS
Full-thickness skin defect can be healed by covering with acellular porcine dermal matrix produced by two methods combined with split-thickness autograft, and it can help improve the quality of wound healing.
Ma
Zhong-feng
ZF
Department of Burns and Plastic Surgery, Burns Institute, 304th Clinical Department of General Hospital of PLA, Beijing 100037, China.
Chai
Jia-ke
JK
Yang
Hong-ming
HM
Liu
Qiang
Q
Xu
Ming-huo
MH
Yin
Hui-nan
HN
D000818:Animals; D020405:Dermis; D004195:Disease Models, Animal; D005260:Female; D006085:Graft Survival; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D013552:Swine; D014945:Wound Healing