Enhanced repair of large osteochondral defects using a combination of artificial cartilage and basic fibroblast growth factor.
The purpose of this study was to examine the efficacy of a combination of artificial cartilage and basic fibroblast growth factor (bFGF) for the repair of large osteochondral defects. The artificial cartilage was a three-dimensional fabric (3-DF) composed of an ultra-high molecular weight polyethylene fiber with a triaxial three-dimensional structure. We implanted 3-DF impregnated with type I collagen gel containing 500 ng of bFGF (bFGF-treated group) or 3-DF impregnated with type I collagen gel alone (non-treated group) into a large full-thickness osteochondral defect (6 x 6 x 3 mm) of the patellar groove of rabbits. The defect area was examined grossly, histologically and biomechanically 4-48 weeks after surgery. Bone ingrowth into and around the 3-DF was evaluated with micro-computed tomography (micro-CT). Addition of bFGF to the 3-DF greatly accelerated cartilage formation on the articular surface and subchondral bone formation into and around the 3-DF, and improved biomechanical properties. These findings suggest that a combination of artificial cartilage and bFGF is clinically useful in cases involving large osteochondral defects.
Fukuda
Aki
A
Department of Orthopaedic Surgery, Mie University Faculty of Medicine, 2-174 Edobashi, Tsu city, Mie 514-8507, Japan. aki0611@clin.medic.mie-u.ac.jp
Kato
Ko
K
Hasegawa
Masahiro
M
Hirata
Hitoshi
H
Sudo
Akihiro
A
Okazaki
Kenshi
K
Tsuta
Kaoru
K
Shikinami
Yasuo
Y
Uchida
Atsumasa
A
D000818:Animals; D001187:Artificial Organs; D001672:Biocompatible Materials / Q000737:chemistry; D002358:Cartilage, Articular / Q000187:drug effects / Q000293:injuries* / Q000503:physiopathology* / Q000601:surgery; D024042:Collagen Type I / Q000737:chemistry*; D003131:Combined Modality Therapy; D019245:Compressive Strength; D004343:Drug Implants / Q000008:administration & dosage*; D005260:Female; D005264:Femoral Fractures / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D006244:Hardness; D008422:Materials Testing; D011817:Rabbits; D016896:Treatment Outcome
D001672:Biocompatible Materials; D024042:Collagen Type I; D004343:Drug Implants; D016222:Fibroblast Growth Factor 2