Mannosylated PLP(139-151) induces peptide-specific tolerance to experimental autoimmune encephalomyelitis.
SJL mice immunized with mannosylated (M-) PLP(139-151) in complete adjuvant do not develop EAE and little CNS mononuclear cell infiltration; other mannosylated peptides were ineffective in this experimental setting. Despite apparently normal T cell responses, M-PLP(139-151)-immunized mice show impaired delayed-type-sensitivity to PLP(139-151) but a normal response to other peptides. After re-immunization with PLP(139-151) in complete adjuvant, these mice are largely tolerant to EAE, show less T cell proliferation and decreased peptide-specific IgG2a. Our data suggest that M-PLP(139-151) induces peptide-specific tolerance to EAE via a mechanism of deletion or impaired migration of encephalitogenic T cells.
Luca
Mariken E
ME
Division of Biomedical Research, TNO Prevention and Health, Zernikedreef 9, 2333 CK Leiden, The Netherlands.
Kel
Junda M
JM
van Rijs
Wouter
W
Wouter Drijfhout
Jan
J
Koning
Frits
F
Nagelkerken
Lex
L
D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000276:immunology / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000473:pathology; D016056:Immunodominant Epitopes / Q000276:immunology; D007279:Injections, Subcutaneous; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008358:Mannose / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D018991:Myelin Proteolipid Protein / Q000008:administration & dosage* / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000276:immunology* / Q000378:metabolism; D017634:Self Tolerance / Q000276:immunology*; D012720:Severity of Illness Index; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology
D016056:Immunodominant Epitopes; D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; C435023:myelin proteolipid protein (139-151); D008358:Mannose