Purification and cDNA cloning of NADPH-dependent aldoketoreductase, involved in asymmetric reduction of methyl 4-bromo-3-oxobutyrate, from Penicillium citrinum IFO4631.
Penicillium citrinum was found to catalyze the reduction of methyl 4-bromo-3-oxobutyrate to methyl (S)-4-bromo-3-hydroxybutyrate [(S)-BHBM] with high optical purity. From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated. Escherichia coli cells overexpressing KER produced (S)-BHBM in the presence of an NADPH-regeneration system.
Asako
Hiroyuki
H
Organic Synthesis Research Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan. asako@sc.sumitomo-chem.co.jp
Wakita
Ryuhei
R
Matsumura
Kenji
K
Shimizu
Masatoshi
M
Sakai
Jun
J
Itoh
Nobuya
N
D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism
D018076:DNA, Complementary; D006885:Hydroxybutyrates; C496653:methyl-4-bromo-3-hydroxybutyrate; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase