title: Protein domain of unknown function DUF1023 is an alpha/beta hydrolase.
abstract: Pfam family DUF1023 consists entirely of uncharacterized proteins generated by sequencing the genomes of Actinobacteria (Bateman A., et al., Nucleic Acids Res. 2004;32 Database issue:D138-141.) Utilizing sequence similarity detection methods, we infer homology between DUF1023 and alpha/beta hydrolases. DUF1023 proteins conserve the core secondary structures in alpha/beta hydrolase fold, and share similar catalytic machinery as that of alpha/beta hydrolases. We predict DUF1023 spatial structure and deduce that they function as hydrolases utilizing catalytic Ser-His-Asp triad with the serine as a nucleophile.
authors.affiliation: Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas, Texas 75390-9038, USA.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Zheng
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Ginalski
authors.forename: Leszek
authors.initials: L
authors.lastname: Rychlewski
authors.forename: Nick V
authors.initials: NV
authors.lastname: Grishin
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D030562:Databases, Protein; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D020103:Rhizomucor / Q000201:enzymology
chemical_list: D029742:Caenorhabditis elegans Proteins; D006867:Hydrolases; D008049:Lipase