[Comparison study on inhibitory effect of extracts from leaves of Ginkgo biloba L. on the oxidation of human low density lipoproteins].
OBJECTIVE
To compare the effects of extracts from leaves of Ginkgo biloba L. (EGB761), ginkgo flavonoids (GF) and ginkgo terpenlactones (GT) on the oxidation of human plasma low density lipoproteins (LDL) induced by Fe2+ or Cu2+.


METHODS
LDL was pretreated with EGB761, GF and GT, then was oxidized by CuSO4 or FeSO4 6 micromol/L for 4 h. Fluorescence Spectroscopy methods were adopted to determine the contents of lipofusion (LF), malondialdehyde (MDA) and vitamin E (VitE) of LDL oxidized by Fe2+ or Cu2+ and protected by EGB761, GF or GT.


RESULTS
After the oxidation of LDL induced by Fe2+ or Cu2+, VitE was exhausted, while MDA and LF were markedly increased. EGB761, GF and GT prevented the decrease of VitE and increases of MDA and LF in the ox-LDL. The inhibitory effect was GF > EGB761 > GT.


CONCLUSIONS
GF played the main role in anti-oxidation.
Huang
Peili
P
Faculty of Public Health, Capital University of Medical Sciences, Beijing 100054.
Li
Jianxin
J
Zhang
Shuhua
S
Wang
Hui
H
D000975:Antioxidants / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D020441:Ginkgo biloba; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000975:Antioxidants; D005419:Flavonoids; D008077:Lipoproteins, LDL; D010936:Plant Extracts