title: Novel development-related alternative splices in human testis identified by cDNA microarrays.
abstract: Alternative splicing of premessenger RNA is an important regulatory mechanism that increases the diversity of proteins transcribed from a single gene. This is particularly important in the testis because germ cell expansion and differentiation require many cellular changes and regulatory steps. To investigate novel development-related alternative splicings in the human testis, complementary DNA microarray studies were conducted with the use of probes from human fetal testes, adult testes, and human spermatozoa. Of a total of 386 Unigene clusters found to be related to the development of the testis, 67 clusters showed a total of 74 novel alternative spliceoforms. Developmental stage-dependent expression was also performed for a novel Unigene, NYD-SP20 (Hs.351068), which had 4 possible novel spliceoforms and another Unigene, CRISP2 (cysteine-rich secretory protein 2, Hs.2042), which had 3 possible novel spliceoforms. These results indicate that alternative splicing plays an important role in the complicated processes of testis development and spermatogenesis.
authors.affiliation: Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Junhua
authors.initials: J
authors.lastname: Xiao
authors.forename: Lanlan
authors.initials: L
authors.lastname: Yin
authors.forename: Hu
authors.initials: H
authors.lastname: Zhu
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000235:genetics*; D017398:Alternative Splicing; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000502:physiology*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger