[Effect on synthesis of nitric oxide in myocardium by local cryoablation].
To study the effect on synthesis of nitric oxide in myocardium by local cryoablation and to investigate its mechanism.



Myocardium was cryoablated locally by a probe cooled to -60 degrees C and rewarmed by normal salt solution, nitric oxide and its synthesis enzyme were measured before and after cryoablation. L-arginine or methylene blue was added before and during cryoablation and the effect of these drugs on synthesis of nitric oxide was studied.



Nitric oxide and its synthesis enzyme decreased after cryoablation; L-arginine preserved the synthesis of nitric oxide and methylene blue inhibited the synthesis of nitric oxide. However, nitric oxide in serum did not change.



Nitric oxide and its synthesis enzyme in myocardium decrease after cryoablation.
Zhao
Bai-qin
BQ
The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Zhu
Jia-guang
JG
Zhang
Ming
M
Cheng
Hai-feng
HF
Fan
Jun-qiang
JQ
D000818:Animals; D003452:Cryosurgery; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011817:Rabbits
D009569:Nitric Oxide; D019001:Nitric Oxide Synthase