Functional genomics using high-throughput RNA interference.
RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells.
Vanhecke
Dominique
D
Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Fabeckstrasse 60-62, 14195 Berlin, Germany.
Janitz
Michal
M
D000818:Animals; D020869:Gene Expression Profiling; D015723:Gene Library; D020868:Gene Silencing; D023281:Genomics; D006801:Humans; D046228:Microarray Analysis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000502:physiology
D034741:RNA, Small Interfering