[Inhibition of phospholipase C gamma1 signaling pathway promotes apoptosis of human colorectal carcinoma cells].
OBJECTIVE
To investigate the effects of inhibiting phospholipase C gamma1 signaling pathway on the apoptosis of human colorectal carcinoma cells.


METHODS
SW620 cells were treated with U73122 in vitro to inhibit the phospholipase C gamma1 signalling pathway and examined under light microscope and transmission electron microscope for analyzing changes in apoptotic behavior of the cells. MTT assay was used to evaluate the cell killing effects, and the percentage of apoptotic cells analyzed using flow cytometry.


RESULTS
After inhibition of the phospholipase C gamma1 signaling pathway by U73122, SW620 cells exhibited obvious apoptotic morphology, the viable cells decreased dramatically, and the percentage of apoptotic cells rose to above 50%.


CONCLUSIONS
Inhibition of phospholipase C gamma1 signaling pathway can induce apoptosis of human colorectal carcinoma cells.
Liu
Jun
J
Department of Cell Biology, Southern Medical University, Guangzhou 510515, China.
Li
Ming
M
Zeng
Wei-Sen
WS
Bai
Xiao-Chun
XC
Luo
Shen-Qiu
SQ
D017209:Apoptosis / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000473:pathology*; D004963:Estrenes / Q000494:pharmacology*; D006801:Humans; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D051966:Phospholipase C gamma / Q000037:antagonists & inhibitors*; D011760:Pyrrolidinones / Q000494:pharmacology*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D004963:Estrenes; D010726:Phosphodiesterase Inhibitors; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D051966:Phospholipase C gamma