[Determination of sennosides and degraded products in the process of sennoside metabolism by HPLC].
A method for the separation and determination of sennosides A and B and the main composition (sennidins A and B) in degraded products of sennosides by linear gradient high performance liquid chromatography has been developed. Separation conditions were as follows: column, a Spherisorb C18 column (250 mm x 4.6 mm i.d., 10 microm); column temperature, 40 degrees C; detection wavelength, 360 nm; mobile phase A, 1.25% acetic acid aqueous solution; mobile phase B, methanol; linear gradient, 100% A --> (20 min) 100% B. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of real-time analysis in the process of sennoside metabolism.
Sun
Yan
Y
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China. sunyan@dicp.ac.cn
Li
Xuetuo
X
Yu
Xingju
X
D000880:Anthraquinones / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D012676:Senna Extract / Q000032:analysis / Q000737:chemistry; D000081226:Sennosides
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D000081226:Sennosides; D012676:Senna Extract