Role of CpG islands in the up-regulation of NMDA receptor NR2B gene expression following chronic ethanol treatment of cultured cortical neurons of mice.
Our earlier studies have indicated that chronic ethanol treatment produces an increase in the rate of NR2B gene expression but acute ethanol treatment doesn't cause any change in the expression in adult cortex and cultured fetal cortical neurons. To determine the molecular mechanism involved in the up-regulation of NR2B gene expression, we studied methylation in CpG islands in the regions (5843-6276) and (6477-6763) in mouse fetal cortical cultured neurons. However, acute administration of ethanol (3.5 g/kg, i.p.) as a 25% solution diluted in 0.9% saline to the adult mice did not cause any change in the methylation status of the NR2B gene. Therefore, these observations indicate that chronic ethanol causes demethylation in CpG islands in the regions (5843-6276) and (6477-6763), thereby probably resulting in increased expression of the NR2B gene. In contrast, acute ethanol treatment did not cause any change in the NR2B gene expression or its demethylation. We also investigated the changes in hypersensitive sites in CpG islands and transcription initiation sites in the cortical cultured neurons following chronic ethanol treatment. These experiments revealed that chronic ethanol treatment does not alter the pattern of hypersensitive sites in adult cortex and cultured cortical neurons.
Marutha Ravindran
C R
CR
Department of Pharmacology, The University of Texas Health Science Center, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA.
Ticku
Maharaj K
MK
D000375:Aging / Q000235:genetics / Q000378:metabolism; D020268:Alcohol-Induced Disorders, Nervous System / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D018899:CpG Islands / Q000187:drug effects* / Q000235:genetics; D019175:DNA Methylation / Q000187:drug effects*; D003850:Deoxyribonuclease I / Q000235:genetics / Q000494:pharmacology; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D000431:Ethanol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
C121001:NR2B NMDA receptor; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D000431:Ethanol; D003850:Deoxyribonuclease I