Common co-lipids, in synergy, impart high gene transfer properties to transfection-incompetent cationic lipids.
Efficacious cationic transfection lipids usually need either DOPE or cholesterol as co-lipid to deliver DNA inside the cell cytoplasm in non-viral gene delivery. If both of these co-lipids fail in imparting gene transfer properties, the cationic lipids are usually considered to be transfection inefficient. Herein, using both the reporter gene assay in CHO, COS-1 and HepG2 cells and the whole cell histochemical X-gal staining assay in representative CHO cells, we demonstrate that common co-lipids DOPE, Cholesterol and DOPC, when act in synergy, are capable of imparting improved gene transfer properties to a novel series of cationic lipids (1-5). Contrastingly, lipids 1-5 became essentially transfection-incompetent when used in combination with each of the pure co-lipid components alone.
Mukherjee
Koushik
K
Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad 500 007, India.
Sen
Joyeeta
J
Chaudhuri
Arabinda
A
D000818:Animals; D002412:Cations / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000494:pharmacology; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D015394:Molecular Structure; D010316:Particle Size; D010713:Phosphatidylcholines / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D014162:Transfection / Q000295:instrumentation* / Q000379:methods*
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D002412:Cations; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D004247:DNA; D002784:Cholesterol; D003850:Deoxyribonuclease I; C017251:1,2-oleoylphosphatidylcholine