[Therapeutic effect of cationic liposome-mediated interleukin-12 gene delivery on murine melanoma in vivo].
To investigate the therapeutic effect of cationic liposome-mediated interleukin-12 gene delivery on established murine melanoma in vivo.



The lipofectin encapsulated pCmIL-12 plasmid was given to C57BL/6 mice on the day 3,5,7,9 after inoculation of B16 melanoma cells. The tumor size, the survival time of mice and the NK cell activity were observed.



The pCmIL-12 plasmid coupled with cationic liposome inhibited the tumor growth and improved the survival of mice bearing established melanoma. The activity of NK cells was also enhanced after interleukin-12 gene delivery in vivo.



Cationic liposome-mediated interleukin-12 gene delivery has significantly therapeutic effects on mice melanoma in vivo.
Chen
Wei
W
Institute of Immunology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Shao
Chuan-sen
CS
Shen
Jian-gen
JG
Pan
Yi
Y
Zheng
Shu
S
D000818:Animals; D002412:Cations; D004247:DNA / Q000627:therapeutic use; D005260:Female; D018664:Interleukin-12 / Q000235:genetics / Q000627:therapeutic use*; D007694:Killer Cells, Natural / Q000276:immunology; D008081:Liposomes; D008546:Melanoma, Experimental / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured
D002412:Cations; D008081:Liposomes; D018664:Interleukin-12; D004247:DNA