[Influence of mobile phase composition on chiral separation of albendazole sulfoxide racemates].
A chiral stationary phase (CSP) was prepared by coating amylose-tris(3,5-dimethylphenyl carbamate) (ADMPC) onto aminopropylated spherical silica gel. Albendazole sulfoxide (ABZSO) was directly resolved on the ADMPC-CSP by normal-phase high performance liquid chromatography (HPLC) using a series of binary and ternary mobile phases. The results indicated that the retention and stereoselectivity of ABZSO on ADMPC-CSP were significantly influenced by the kinds and the contents of the alcohols in mobile phases. The use of methanol and/or ethanol as alcoholic modifier achieved better separation of ABZSO. Effective and efficient chiral resolution was obtained by using ternary mobile phases: hexane/2-propanol/methanol or hexane/ethanol/methanol.
Zhai
Zongde
Z
Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China. zzdlz@yahoo.com.cn
Zhang
Hong
H
Dun
Huijuan
H
Liu
Chunhui
C
Chen
Liren
L
Li
Yongmin
Y
D019840:2-Propanol / Q000737:chemistry; D015766:Albendazole / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D000871:Anthelmintics / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry; D007536:Isomerism; D000432:Methanol / Q000737:chemistry; D013237:Stereoisomerism
D000871:Anthelmintics; D006586:Hexanes; D000431:Ethanol; D015766:Albendazole; C027186:albendazole sulfoxide; D019840:2-Propanol; D000432:Methanol