Dystrophin Dp71 in PC12 cell adhesion.
Previously, we reported that PC12 cells with decreased Dp71 expression (antisense-Dp71 cells) display deficient nerve-growth-factor-induced neurite outgrowth. In this study, we show that disturbed neurite outgrowth of antisense-Dp71 cells is accompanied by decreased adhesion activity on laminin, collagen and fibronectin. In wild-type cells, the immunostaining of Dp71 and beta1-integrin overlaps in the basal area contacting the substrate, but staining of both proteins decrease in the antisense-Dp71 cells. Morphology of antisense-Dp71 cells at the electron microscopic level is characterized by the lack of filopodia, cellular projections involved in adhesion. Our findings suggest that Dp71 is required for the efficient PC12 cell attachment to beta1-integrin-dependent substrata and that decreased adhesion activity of the antisense-Dp71 cells could determine their deficiency to extend neurites.
Enríquez-Aragón
Jose Arturo
JA
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN, 07360 México, D. F., México.
Cerna-Cortés
Joel
J
Bermúdez de León
Mario
M
García-Sierra
Francisco
F
González
Everardo
E
Mornet
Dominique
D
Cisneros
Bulmaro
B
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000187:drug effects / Q000502:physiology; D016189:Dystrophin / Q000031:analogs & derivatives* / Q000502:physiology*; D005353:Fibronectins / Q000187:drug effects / Q000502:physiology; D005455:Fluorescent Antibody Technique / Q000379:methods; D039641:Integrin beta Chains / Q000378:metabolism; D007797:Laminin / Q000187:drug effects / Q000502:physiology; D008855:Microscopy, Electron, Scanning / Q000379:methods; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D018709:Statistics, Nonparametric
D016189:Dystrophin; D005353:Fibronectins; D039641:Integrin beta Chains; D007797:Laminin; D020319:Oligodeoxyribonucleotides, Antisense; C085339:apo-dystrophin 1; D003094:Collagen