title: Helicobacter pylori heat-shock protein 60 induces production of the pro-inflammatory cytokine IL8 in monocytic cells.
abstract: Interleukin 8 (IL8) is usually produced in both epithelial and monocytic cells during bacterial infections, causing inflammation. Helicobacter pylori induces production of IL8 from gastric epithelial cells via its cag pathogenicity island (cag PAI) system, LPS and outer-membrane protein. In some bacteria, heat-shock protein 60 (HSP60) also elicits a strong pro-inflammatory response in cells of the innate immune system. Three recombinant H. pylori HSP60 (rHSP60) proteins of different sizes were produced and one of these was used to raise two monoclonal antibodies (2E7 and 7B5). IL8 production was found to be induced in cultured monocytic cells treated with H. pylori cells or rHSP60 proteins, as measured by ELISA, and the amount of IL8 produced was dose-dependent. Pre-incubation of H. pylori cells or rHSP60 preparations with the antibody 2E7 significantly inhibited IL8 production from monocytic cells. These results indicated that HSP60 is closely associated with IL8 production in monocytic cells.
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Song-Nan
authors.initials: SN
authors.lastname: Lin
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ayada
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yokota
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Takenaka
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Kan
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Shyunji
authors.initials: S
authors.lastname: Hayashi
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Motowo
authors.initials: M
authors.lastname: Mizuno
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Hirai
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Fujinami
authors.affiliation: Departments of Bacteriology1, Dermatology2 and Medicine and Medical Science3, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan 4Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji, Minami-Kawauchi-cho, Kouchi-Gun, Tochigi 329-0498, Japan 5Gastroenterology, Hiroshima City Hospital, 7-33 Moto-cho, Naka-ku, Hiroshima 730-8518, Japan 6National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa 277-0882, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Oguma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002460:Cell Line; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005260:Female; D016480:Helicobacter pylori / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020298:U937 Cells
chemical_list: D000911:Antibodies, Monoclonal; D018834:Chaperonin 60; D016209:Interleukin-8; D010455:Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins