title: Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana.
abstract: A novel Arabidopsis thaliana gene (AtNADK-1) was identified based on its response to radiation and oxidative stress. Levels of AtNADK-1 mRNA increase eight-fold following exposure to ionising radiation and are enhanced three-fold by treatment with hydrogen peroxide. The gene also appears to be differentially regulated during compatible and incompatible plant-pathogen interactions in response to Pseudomonas syringae pv. tomato. The full-length AtNADK-1 cDNA encodes a 58-kDa protein that shows high sequence homology to the recently defined family of NAD(H) kinases. Recombinant AtNADK-1 utilises ATP to phosphorylate both NAD and NADH, showing a two-fold preference for NADH. Using reverse genetics, we demonstrate that AtNADK-1 deficient plants display enhanced sensitivity to gamma irradiation and to paraquat-induced oxidative stress. Our results indicate that this novel NAD(H) kinase may contribute to the maintenance of redox status in Arabidopsis thaliana.
authors.affiliation: Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire de Bioénergétique Cellulaire, CEA Cadarache, Bât 156, 13108 Saint-Paul Lez Durance, France.
authors.forename: Jean-Guy
authors.initials: JG
authors.lastname: Berrin
authors.forename: Olivier
authors.initials: O
authors.lastname: Pierrugues
authors.forename: Catherine
authors.initials: C
authors.lastname: Brutesco
authors.forename: Béatrice
authors.initials: B
authors.lastname: Alonso
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Montillet
authors.forename: Dominique
authors.initials: D
authors.lastname: Roby
authors.forename: Michael
authors.initials: M
authors.lastname: Kazmaier
mesh_terms: D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000528:radiation effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D044224:Pseudomonas syringae; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D006861:Hydrogen Peroxide; D010770:Phosphotransferases; C500558:NADK-1 protein, Arabidopsis; D017853:Phosphotransferases (Alcohol Group Acceptor)