Hybrid lethality of cultured cells of an interspecific F1 hybrid of Nicotiana gossei Domin and N. tabacum L.
Cultured cells were established from the hypocotyl of F(1) hybrid seedlings of Nicotiana gossei Domin and N. tabacum L. The cultured cells started to die at 26 degrees C, but not at 37 degrees C, which is similar to what occurred in cells of the original hybrid plants. An increase in the number of cells without cytoplasmic strands and acidification of the cytoplasm followed by decomposition of the mitochondria and chloroplasts indicated that vacuolar collapse plays a central role in the execution of cell death. Oxygen but not light was required for cell death. Cellular levels of the superoxide anion and hydrogen peroxide temporarily increased during the early phase at 26 degrees C, while no such oxidative burst was observed at 37 degrees C. The reactive oxygen intermediates are potentially involved in the death of the hybrid cells.
Mino
Masanobu
M
Graduate School of Agriculture, Kyoto Prefectural University, Shimogamo, Sakyo-ku, Japan. mino@kpu.ac.jp
Misaka
Yuko
Y
Ueda
Junko
J
Ogawa
Ken'ichi
K
Inoue
Masayoshi
M
D016923:Cell Death; D002678:Chimera; D002736:Chloroplasts / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008027:Light; D008928:Mitochondria / Q000502:physiology; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism; D013696:Temperature; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D014617:Vacuoles / Q000502:physiology
D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide