Development of a cDNA array for chicken gene expression analysis.
BACKGROUND
The application of microarray technology to functional genomic analysis in the chicken has been limited by the lack of arrays containing large numbers of genes.


RESULTS
We have produced cDNA arrays using chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. From a total of 363,838 chicken ESTs representing 24 different adult or embryonic tissues, a set of 11,447 non-redundant ESTs were selected and added to an existing collection of clones (4,162) from immune tissues and a chicken bursal cell line (DT40). Quality control analysis indicates there are 13,007 useable features on the array, including 160 control spots. The array provides broad coverage of mRNAs expressed in many tissues; in addition, clones with expression unique to various tissues can be detected.


CONCLUSIONS
A chicken multi-tissue cDNA microarray with 13,007 features is now available to academic researchers from http://genomics@fhcrc.org. Sequence information for all features on the array is in GenBank, and clones can be readily obtained. Targeted users include researchers in comparative and developmental biology, immunology, vaccine and agricultural technology. These arrays will be an important resource for the entire research community using the chicken as a model.
Burnside
Joan
J
Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Room 229, Newark, DE 19711, USA. joan@udel.edu
Neiman
Paul
P
Tang
Jianshan
J
Basom
Ryan
R
Talbot
Richard
R
Aronszajn
Mark
M
Burt
David
D
Delrow
Jeff
J
D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D016000:Cluster Analysis; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism*; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D023281:Genomics; D051379:Mice; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger