Relative expression and localization of the insulin-like growth factor system components in the fetal, child and adult intestine.
BACKGROUND
The insulin-like growth factors (IGFs) are important in the development and maintenance of the gastrointestinal tract.


OBJECTIVE
To compare the expression of IGFs and their receptors in the stomach and duodenum of the fetus, the child and the adult. To identify the cells mainly responsible for the production of the members of the IGF system.


METHODS
Tissue was obtained from fetus after abortion and from children and adults during diagnostic endoscopy and biopsy. The expression of the IGFs and their receptors was estimated by an RNAse protection assay and sections were stained with antisera to the components of IGF system.


RESULTS
The tissues from the stomach and the duodenum expressed the two IGFs and their receptors at all stages of life. The fetal IGF receptors I and II, were approximately ten times higher than in the child and IGF-II was five times higher. Immunohistochemical staining showed the components of the IGF system to be localized to the gastric glands and to the basotlateral border of the gastric epithelial cells. In the duodenum, they were concentrated at the apical portion of the epithelial tissue. They could also be identified in ganglion cells and nerves.


CONCLUSIONS
The IGFs and their receptors in the stomach and duodenum are expressed in all age groups and mostly are highest in the fetus. The IGF system proteins were located in the gastric glands and epithelium and in the apical portion of the villous epithelium of the duodenum.
Freier
Serem
S
Pediatric Gastroenterology Unit, Shaare Zedek Medical Centre, Jerusalem, 91031 Israel. zerem@zahav.net.il
Eran
Maya
M
Reinus
Constantin
C
Ariel
Ilana
I
Faber
Joseph
J
Wilschanski
Michael
M
Braverman
Danny
D
D037881:Aborted Fetus / Q000378:metabolism*; D000328:Adult; D001706:Biopsy; D002675:Child, Preschool; D005753:Gastric Mucosa / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D017451:Receptors, Somatomedin / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D013002:Somatomedins / Q000378:metabolism*; D013270:Stomach / Q000473:pathology
D012333:RNA, Messenger; D017451:Receptors, Somatomedin; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D012260:Ribonucleases