[Study on purification of total flavonoids from seed residue of Hippophae rhamnoides with macroporous resin].
OBJECTIVE
To study the conditions and parameters of purifying total flavonoids from seed residue of H. rhamnoides.


METHODS
Absorption capacity of three resins for total flavonoids was compared. With the yield and purity as indexes, the process of absorbing and purifying total flavonoids from seed residue with D101 macroporous resin absorbent was selected by orthogonal design.


RESULTS
The D101 resin was the best of the three resins and its absorption capacity was determined to be 63.00 mg x g(-1) wet resin. The optimum process condition was 30% ethanol as eluting solvent, wet resin vs crude herbs: 2:1, diameter vs height: 1:10, eluting solvent vs crude herbs: 10:1, absorbing time for 3 h. The yield of total flavonoids from seeds residue of H. rhamnoides by this process was 2.39%, and the purity reached 64.81%.


CONCLUSIONS
The process is simple and convenient and the regeneration of resin is easy. So this method of purification is advisable.
Cao
Qun-hua
QH
Life Sciences School of East China Normal University, Shanghai, 200062, China.
Qu
Wei-jing
WJ
Li
Jia-gui
JG
Deng
Yun-xia
YX
D000431:Ethanol; D005419:Flavonoids / Q000302:isolation & purification*; D031281:Hippophae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D012639:Seeds / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*
D005419:Flavonoids; D012117:Resins, Synthetic; D000431:Ethanol