Chemoenzymatic synthesis of CD52 glycoproteins carrying native N-glycans.
A facile synthesis of homogeneous CD52 glycoproteins carrying native N-glycans was achieved using an endolycosidase-catalyzed oligosaccharide transfer as the key step. The synthesis consists of two steps: the solid phase synthesis of GlcNAc-CD52 and the transfer of a high-mannose type or complex type N-glycan from Man(9)GlcNAc(2) Asn or a sialglycopeptide to the GlcNAc-CD52, under the catalysis of the endo-beta-N-acetylglucosaminidases from Arthrobacter (Endo-A) and Mucor hiemalis (Endo-M), respectively.
Li
Hengguang
H
Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, 725 West Lombard Street, Baltimore, MD 21201, USA.
Singh
Suddham
S
Zeng
Ying
Y
Song
Haijing
H
Wang
Lai-Xi
LX
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D001173:Arthrobacter / Q000201:enzymology; D000074301:CD52 Antigen; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000138:chemical synthesis*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006031:Glycosylation; D009089:Mucor / Q000201:enzymology; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D006026:Glycoside Hydrolases