title: [Screening of angiotensin converting enzyme inhibitors from Salvia miltiorrhizae].
abstract: OBJECTIVE
  To screen angiotensin converting enzyme inhibitor (ACEI) from traditional Chinese medicine, Salvia miltiorrhiza.
  
  
  METHODS
  Hydrophilic and lipophilic fractions of S. miltiorrhiza were isolated, and their effective components were screened by a fluorimetric assay for inhibition of angiotensin converting enzyme (ACE).
  
  
  RESULTS
  Water-soluble fractions, total salvianolic acids and salvianolic acid B markedly decreased ACE activity of rabbit lung tissue. Their IC50 value were (2.45 +/- 0.07), (0.24 +/- 0.02), (0.02 +/- 0.01) g x L(-1) respectively. Lipophilic components or phenanthraquinones including tanshinone I and II showed no changes on the activity of ACE.
  
  
  CONCLUSIONS
  S. miltiorrhiza inhibits angiotensin converting enzyme and its active components are in aqueous extract, in which the main were salvianolic acids including salvianolic acids B.
authors.affiliation: Institute of Materia Medica, Chengdu DIAO Group, Chengdu 610041, China. gxplww@hotmail.com
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Gao
authors.forename: Da-yong
authors.initials: DY
authors.lastname: Xu
authors.forename: Yi-long
authors.initials: YL
authors.lastname: Deng
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry; D053630:Depsides
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D007703:Peptidyl-Dipeptidase A; C076944:salvianolic acid B; D053630:Depsides