Dynamics of single polymers in a stagnation flow induced by electrokinetics.
An electrokinetics-induced stagnation flow was created inside a microscale cross-channel. Compared to hydrodynamic-induced microfluidics, this flow system can be readily assembled and the operation is very simple due to a low pressure drop. Through image analysis, a fairly homogeneous, two-dimensional elongational flow was observed. The initial conformation of DNA molecules and residence time inside the flow field play important roles in determining the extent of DNA stretching. A coarse-grain molecular simulation agrees reasonably well with experimental observations.
Juang
Y-J
YJ
Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
Wang
S
S
Hu
X
X
Lee
L J
LJ
D003198:Computer Simulation; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004548:Elasticity; D004563:Electrochemistry / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D008846:Micromanipulation / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013314:Stress, Mechanical
D004247:DNA