title: [The multiplex analysis of epigenetic markers and genetic markers by post-digestion mutagenically separated PCR].
abstract: OBJECTIVE
  To establish a novel method for the multiplex analysis of the methylation and single nucleotide polymorphism (SNP).
  
  
  METHODS
  The imprinted SNP rs220028 was chosen as a model. Genomic DNA, after being digested with methylation sensitive restriction enzyme, were typed by mutagenically separated PCR (MS-PCR). The polymorphism of restriction site was excluded by PCR-RFLP.
  
  
  RESULTS
  By post-digestion MS-PCR, the methylated allele was detected selectively, the maternal origin of which was confirmed by pedigree analysis; A=0.5085, G=0.4915,PIC=0.3749.
  
  
  CONCLUSIONS
  The multiplex analysis of methylation markers and SNP can be achieved by post-digestion MS-PCR. The imprinted SNP locus rs220028 is a potentially useful marker in screening Prader-Willi/Angelman syndrome.
authors.affiliation: Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030 PR China.
authors.forename: Gui-sen
authors.initials: GS
authors.lastname: Zhao
authors.forename: Dai-xin
authors.initials: DX
authors.lastname: Huang
authors.forename: Wen-fang
authors.initials: WF
authors.lastname: Feng
authors.forename: Qing-en
authors.initials: QE
authors.lastname: Yang
mesh_terms: D019175:DNA Methylation; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D005819:Genetic Markers; D004262:DNA Restriction Enzymes