Maintenance of functional human cancellous bone and human hematopoiesis in NOD/SCID mice.
Attempts were made to establish models to study interactions between marrow stromal cells and hematopoietic cells in vivo. The approach was to create a NOD-SCID-hu murine model of long-term human hematopoiesis by implantation of a human adult bone fragment. Nine to 12 weeks posttransplantation, human CD45+ cells were detected in the blood and the spleen of some mice. The histology of the human transplant showed that human bone fragment was viable at 9 weeks. Moreover, vessels of human origin, as assessed by immunohistochemical detection of human beta2-microglobulin, were observed in the mouse tissue surrounding the transplanted human fragment.
Hubin
Frédérique
F
Department of Cytology and Histology, University of Liège, Liège, Belgium. F.Hubin@ulg.ac.be
Humblet
Chantal
C
Belaid
Zakia
Z
Greimers
Roland
R
Boniver
Jacques
J
Thiry
Albert
A
Defresne
Marie-Paule
MP
D000293:Adolescent; D000328:Adult; D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000379:methods*; D016025:Bone Transplantation / Q000276:immunology / Q000379:methods*; D002450:Cell Communication / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D006410:Hematopoiesis / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D016867:Immunocompromised Host / Q000502:physiology*; D017493:Leukocyte Common Antigens / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D023421:Models, Animal; D013154:Spleen / Q000166:cytology / Q000276:immunology; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology; D023001:Transplantation Tolerance / Q000276:immunology*; D014183:Transplantation, Heterologous / Q000276:immunology / Q000379:methods*; D001613:beta 2-Microglobulin / Q000276:immunology
D001613:beta 2-Microglobulin; D017493:Leukocyte Common Antigens