4,4'-Dihydroxybiphenyl as a new potent tyrosinase inhibitor.
The color of mammalian skin is determined by many factors, for which visible ones are the degree and distribution of melanin pigmentation. Because tyrosinase, (polyphenol oxidase) is the key enzyme for melanin biosynthesis, the use of various tyrosinase inhibitors is a common practice for whitening purpose in cosmetics. In the present study, the inhibition of tyrosinase by 4,4'-dihydroxybiphenyl (44'-BP) was investigated. In addition to tyrosinase inhibiting activity, melanin biosynthesis was assessed in B16F10 melanoma cells (B16 cells). The results showed that 44'-BP exhibits a strong anti-tyrosinase activity with IC50=1.91 microM. The kinetic analysis of tyrosinase inhibition revealed that 44'-BP acts a competitive inhibitor (Ki=4.0 x 10(-4) M at 2.5 microM and Ki =21 x 10(-5) M at 5 microM). Furthermore, data on melanin biosynthesis indicated that the amount of melanin was clearly suppressed by 44'-BP.
Kim
You Jung
YJ
Department of Cosmetology, Pusan Women's College, Busanjin-Gu, Busan, 607-843, Korea.
No
Jae Kyung
JK
Lee
Ji Hyeon
JH
Chung
Hae Young
HY
D001713:Biphenyl Compounds / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008546:Melanoma, Experimental / Q000201:enzymology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism
D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D014442:Monophenol Monooxygenase; C027439:4,4'-dihydroxybiphenyl