[Effect of ligustrazine on injury of vascular endothelial cells ECV-304 induced by hypoxia and lack of glucose].
OBJECTIVE
To investigate the effects of ligustrazine on nitric oxide (NO), malonaldehyde (MDA) production, release of intracellular lactate dehydrogenase (LDH) and membrane fluidity of the injured human umbilical vein vascular endothelial cell line (ECV-304) with hypoxia and lack of glucose.


METHODS
The experiments were performed in culture of ECV-304 injured with hypoxia and lack of glucose in vitro. The released LDH of ECV-304 was measured with automatic biochemistry analyse. NO content of ECV-304 was monitored with colorimetry. Lipid peroxidation of ECV-304 was monitored as MDA with a fluorometric assay. The membrane fluidity of ECV-304 was measured with the fluorescence polarization method.


RESULTS
After culture ECV-304 in hypoxia and lack of glucose for 24 h, the LDH release, MDA production and the membrane fluidity increased significantly and NO level was decreased. Preincubation of ECV-304 with ligustrazine for 24 h reduced LDH release, MDA production, membrane fluidity increasing and increased the level of NO in ECV-304 due to hypoxia and lack of glucose.


CONCLUSIONS
Ligustrazine has protective effect on injury of ECV-304 induced by hypoxia and lack of glucose.
Lin
Rong
R
Dept of Pharmacology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. linrong@mail.xjtu.edu.cn
Liu
Jun-tian
JT
Gan
Wei-jie
WJ
Wang
Wei-rong
WR
D015687:Cell Hypoxia; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007003:Hypoglycemia / Q000378:metabolism* / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D030004:Ligusticum / Q000737:chemistry; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology; D011719:Pyrazines / Q000302:isolation & purification / Q000494:pharmacology*
D020011:Protective Agents; D011719:Pyrazines; D009569:Nitric Oxide; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; C017953:tetramethylpyrazine