title: Expressed sequence tags analysis of Atlantic halibut (Hippoglossus hippoglossus) liver, kidney and spleen tissues following vaccination against Vibrio anguillarum and Aeromonas salmonicida.
abstract: To investigate the response of Atlantic halibut to vaccination and pathogen exposure, a cDNA library was constructed from liver, kidney and spleen mRNA collected following vaccination against Vibrio anguillarum and Aeromonas salmonicida. After sequencing 1114 clones 1072 (96.23%) readable sequences were obtained of which 106 sequences are the first reported from the fish. Of these, 182 clones (16.98%) contained cell/organism defence genes including immunoglobulin light chain, MHC class I and II, interferon consensus sequence binding protein, B-cell receptor-associated protein, early B-cell factor, 10 complement components, heat shock protein 70 and 90, antimicrobial peptides hepcidin type 1 and 2, and CC chemokine (macrophage inflammatory protein-1 beta-like chemokine, MIP-1beta). Expression of MIP-1beta-like was elevated in the kidney and spleen at 1, 2, 7 and 14 days post vaccination. Functional genes involved in cellular processes of hematopoietic tissues were also identified. These results indicate that this cDNA library contains many important genes involved in the immune response, making it an important resource for studying the response of Atlantic halibut to vaccination or pathogen exposure.
authors.affiliation: Institute for Marine Biosciences, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. kyoung.park@nrc-cnrc.gc.ca
authors.forename: Kyoung C
authors.initials: KC
authors.lastname: Park
authors.forename: Jane A
authors.initials: JA
authors.lastname: Osborne
authors.forename: Stephen C M
authors.initials: SC
authors.lastname: Tsoi
authors.forename: Laura L
authors.initials: LL
authors.lastname: Brown
authors.forename: Stewart C
authors.initials: SC
authors.lastname: Johnson
mesh_terms: D048409:Aeromonas salmonicida; D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D017931:DNA Primers; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control*; D005432:Flounder / Q000235:genetics*; D005667:Furunculosis / Q000517:prevention & control / Q000662:veterinary*; D015723:Gene Library; D007109:Immunity / Q000235:genetics; D007668:Kidney / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D014611:Vaccination / Q000662:veterinary*; D014735:Vibrio Infections / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D017931:DNA Primers