CD14 C(-260)T promoter polymorphism and prevalence of acute coronary syndromes.
BACKGROUND
Inflammation and infection have been implicated in atherosclerosis and its complications. The CD14 receptor mediates monocyte activation by lipopolysaccharide (LPS) of Gram-negative bacteria. The aim of this study was to assess whether the C(-260)T polymorphism in the promoter of the CD14 receptor gene is associated with a higher prevalence of acute coronary syndromes (ACS) and severity of coronary atherosclerosis.


METHODS
We studied 428 patients (mean age: 63+/-10 years, 67% men) consisting of 334 patients with coronary artery disease (CAD) and 94 patients with normal coronary arteriogram. Patients with CAD were subdivided in two groups: (1) no previous history of ACS (n=140; 64+/-9 years; 79% men) and (2) patients with a history of ACS (n=194; 64+/-10 years; 80% men). CD14 genotypes were determined by a Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism Analysis (RFLP) technique.


RESULTS
Patients with a prior ACS had a significantly higher frequency of the T/T genotype than CAD patients without prior ACS (33% vs. 20%; P=0.009), even after multivariate analysis (odd ratio [OR] 1.8 [1.1-3.1]; confidence intervals [CI] 95%; P=0.023). T/T genotype was not significantly different in CAD patients without prior ACS compared to controls (20% vs. 22.3%; P=0.67), and there was no significant association between genotypes, or allele frequencies, and severity of CAD.


CONCLUSIONS
The CD14 C(-260)T polymorphism is associated with a history of ACS and it may represent a genetically determined risk factor for the development of ACS and atheromatous plaque vulnerability in angina patients.
Arroyo-Espliguero
Ramón
R
Coronary Artery Disease Research Unit, Department of Cardiological Sciences, St. George's Hospital Medical School, Cranmer Terrace, London SW 17 ORE, UK.
El-Sharnouby
Khaled
K
Vázquez-Rey
Eugenia
E
Kalidas
Kamini
K
Jeffery
Steve
S
Kaski
Juan Carlos
JC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003596:Cytosine; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D009203:Myocardial Infarction / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013577:Syndrome; D013941:Thymine
D018950:Lipopolysaccharide Receptors; D003596:Cytosine; D013941:Thymine