Spectrofluorimetric and micelle-enhanced spectrofluorimetric determination of gatifloxacin in human urine and serum.
A spectrofluorimetric method to determine gatifloxacin has been developed and applied to the quantification of this fluoroquinolone in spiked human urine and serum. The native fluorescence of gatifloxacin allow the determination of 0.040-0.700 micro gmL(-1) of this molecule in aqueous solution containing acetic acid-sodium acetate buffer (pH 3.5), with lambda(exc)=292 nm and lambda(em)=484 nm. Micelle-enhanced fluorescence led to 75% higher analytical signals in presence of 12 mM sodium dodecyl sulphate, which allow the determination of 0.020-0.450 microg mL(-1) fluoroquinolone with lambda(exc)=292 nm and lambda(em)=470 nm. Both methods were successfully applied to gatifloxacin determination in spiked human urine and serum.
Ocaña
Juan Antonio
JA
Department of Analytical Chemistry, Faculty of Chemistry, 41012 Seville, Spain.
Barragán
Francisco José
FJ
Callejón
Manuel
M
D024841:Fluoroquinolones / Q000097:blood* / Q000652:urine*; D000077734:Gatifloxacin; D006801:Humans; D007202:Indicators and Reagents; D008823:Micelles; D012680:Sensitivity and Specificity; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D024841:Fluoroquinolones; D007202:Indicators and Reagents; D008823:Micelles; D012996:Solutions; D000077734:Gatifloxacin