title: Chromosomal and genetic aberrations differ with meningioma subtype.
abstract: Meningioma is one of the most common brain tumors, and a variety of genetic abnormalities have been detected by the Southern blotting, polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH) methods. However, these methods detect only a very limited portion of the tumor genome or have a limited mapping resolution. In this study, we used DNA microarray assay, which detects numerous genetic abnormalities and analyzes a global assessment of molecular events in tumor cells. We analyzed genomic DNA from 26 patients with benign meningiomas by GenoSensor Array 300 in order to characterize gene amplifications, gene deletions, and chromosomal information in the whole genome. Loss of chromosome 22q was found most frequently. This chromosomal aberration was detected in 14 meningiomas (53.8%), particularly in transitional and fibrous meningiomas. In meningothelial meningiomas, amplification of INS and TCL1A was detected more frequently than in other meningioma subtypes. DNA microarray assay revealed new genetic differences among the meningioma subtypes, thus indicating that this novel technique is useful for understanding tumor genesis and for the diagnosis of meningioma subtype.
authors.affiliation: Department of Neurosurgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Wada
authors.forename: Motohiko
authors.initials: M
authors.lastname: Maruno
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoki
authors.initials: N
authors.lastname: Kagawa
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hashiba
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Naoya
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Izumoto
authors.forename: Toshiki
authors.initials: T
authors.lastname: Yoshimine
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008579:Meningioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D019394:Ki-67 Antigen