title: [Study on extraction technique of HDCA].
abstract: OBJECTIVE
  To find out the optimal extraction technique for HDCA (a-hydroxycholic acid).
  
  
  METHODS
  According to the orthogonal design L9(3(4)), the optimal extraction technique was sought through experimental investigation, and the content of HDCA was determined by TLC.
  
  
  RESULTS
  The optimal extraction method was eightfold 8% NaOH solution and 16 hours. The optimal purification method was six fold ethyl acetate, 5% active carbon, and 30 minutes twice.
  
  
  CONCLUSIONS
  The above mentioned extraction technique is optimal and feasible in extraction of HDCA.
authors.affiliation: China-Japan Friendship Hospital, Beijing 100029, China. Lujin07091@sina.com
authors.forename: Jin
authors.initials: J
authors.lastname: Lu
authors.forename: Shou-ying
authors.initials: SY
authors.lastname: Du
authors.forename: Li-rui
authors.initials: LR
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D003840:Deoxycholic Acid / Q000032:analysis / Q000302:isolation & purification*; D008419:Materia Medica / Q000032:analysis / Q000302:isolation & purification*; D013552:Swine; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D008419:Materia Medica; D003840:Deoxycholic Acid; C010471:hyodeoxycholic acid