title: [Isolation of beta-carotene from discarded tobacco leaves].
abstract: Beta-carotene was isolated from discarded tobacco leaves by column chromatography using petroleum ether as eluent. Its structure was characterized by 1H-NMR and 13C-NMR spectra. The purity of the beta-carotene was determined to be 98% by a high performance liquid chromatographic (HPLC) method which was demonstrated to be accurate and reproducible. Infra-red spectrum and melting point of the isolated sample were also characterized in agreement with those reported in literature. The overall yield of 80%, the high purity and the easiness to recycle as the results of only a single solvent employed, would make the method an important reference for future research of beta-carotene and a favorable technique for future development.
authors.affiliation: College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Xinzhi
authors.initials: X
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018515:Plant Leaves / Q000737:chemistry; D014026:Nicotiana / Q000737:chemistry*; D019207:beta Carotene / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D019207:beta Carotene