Histomathematical analysis of clinical specimens: challenges and progress.
Proteomic analysis of clinical tissue specimens is a difficult undertaking. Described here is a multiplex study of protein expression levels in histological sections of human prostate that addresses many of the associated challenges. Whole-mount sections from 10 prostatectomy specimens were studied using 15 antibodies, immunohistochemical staining, digital imaging, and mathematical analysis of the data sets. The approach was successful in stratifying cell lineages present in the samples based on proteomic patterns, including differentiating normal epithelium from cancer. This strategy likely will be a useful method for extending the number of proteins that can be analyzed in clinical cancer specimens using currently available laboratory techniques.
Gannot
Gallya
G
Laboratory of Pathology and Urologic Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4605, USA.
Gillespie
John W
JW
Chuaqui
Rodrigo F
RF
Tangrea
Michael A
MA
Linehan
W Marston
WM
Emmert-Buck
Michael R
MR
D004847:Epithelial Cells / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005240:Feasibility Studies; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008433:Mathematics; D025341:Principal Component Analysis; D011467:Prostate / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics
D011506:Proteins