title: Increased oxidative stress in prepubertal severely obese children: effect of a dietary restriction-weight loss program.
abstract: Oxidant-antioxidant status was investigated in a group of severely obese prepubertal children in comparison with healthy subjects and in relation to a dietary restriction-weight loss program. All subjects underwent anthropometric measurements and determination of lipid profile, lag phase, malondialdehyde (MDA), and vitamin E. Compared with controls, obese children had a significantly higher body mass index (BMI) (28.97 +/- 2.42 vs. 16.03 +/- 1.88 kg/m2; P = 0.0002) and waist-to-hip ratio (WHR) (0.89 +/- 0.03 vs. 0.80 +/- 0.01; P = 0.0004); lag phase and vitamin E levels were significantly decreased [24.05 +/- 16.21 vs. 43.16 +/- 10 min (P = 0.004) and 21.12 +/- 14.96 vs. 35.54 +/- 13.62 micromol/liter (P = 0.02), respectively], whereas MDA was significantly increased (0.90 +/- 0.31 vs. 0.45 +/- 0.24 nmol/mg; P = 0.001). Both lag phase and MDA significantly correlated with BMI [respectively, r = -0.34 (P = 0.004) and r = 0.57 (P = 0.002)] and WHR [respectively, r = -0.63 (P = 0.0001) and r = 0.38 (P = 0.04)]. Oxidant status normalized after 6 months of dietary restriction [lag phase, 59.11 +/- 14.74 min (P = 0.002); MDA, 0.47 +/- 0.09 nmol/mg (P = 0.003)], which was associated with a reduction of BMI (27.34 +/- 1.87 kg/m2; P = 0.003), WHR (0.87 +/- 0.02; P = 0.001), and fat mass (34.49 +/- 2.68%; P = 0.008), but returned to baseline levels together with fatness indexes after another 6 months of free diet. Altered oxidant-antioxidant status is already present in prepubertal severely obese children and is reversible with a dietary restriction-weight loss program, which should be highly encouraged and maintained over time in this age group.
authors.affiliation: Department of Pediatrics, University of Chieti, Ospedale Policlinico, Via dei Vestini 5, I-66100 Chieti, Italy. amohn@unich.it
authors.forename: Angelika
authors.initials: A
authors.lastname: Mohn
authors.forename: Mariangela
authors.initials: M
authors.lastname: Catino
authors.forename: Rita
authors.initials: R
authors.lastname: Capanna
authors.forename: Cosimo
authors.initials: C
authors.lastname: Giannini
authors.forename: Maria
authors.initials: M
authors.lastname: Marcovecchio
authors.forename: Francesco
authors.initials: F
authors.lastname: Chiarelli
mesh_terms: D015992:Body Mass Index; D002648:Child; D008078:Cholesterol, LDL / Q000097:blood; D004038:Diet, Reducing; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D009765:Obesity / Q000178:diet therapy* / Q000378:metabolism*; D018384:Oxidative Stress; D011627:Puberty / Q000378:metabolism; D049629:Waist-Hip Ratio; D015431:Weight Loss
chemical_list: D008078:Cholesterol, LDL