Identification of Burkholderia andropogonis with a repetitive sequence BOX element and PCR.
Burkholderia andropogonis is the causal agent of bacterial leaf stripe in sorghum. Strict import quarantine regulations of numerous countries mandate a need for development of a rapid, reliable, and cost-effective diagnostic technique for the identification of B. andropogonis. Using a primer corresponding to the bacterial repetitive BOX element and PCR, we developed a DNA fingerprint which differentiated B. andropogonis from other phytobacterial pathogens.
Ramundo
Bruce A
BA
Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502, USA.
Claflin
Larry E
LE
D019117:Burkholderia / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid