Osmotic shrinkage of cells of Synechocystis sp. PCC 6803 by water efflux via aquaporins regulates osmostress-inducible gene expression.
Osmotic stress causes water molecules to efflux from cells through the cytoplasmic membrane. This study reveals that targeted mutation of the aqpZ gene, encoding an aquaporin water channel protein, in the cyanobacterium Synechocystis sp. PCC 6803 prevents the osmotic shrinkage of cells, suggesting that it is the water channel rather than the lipid bilayer that is primarily responsible for water transition through the membrane of this organism. The observations suggest that the aquaporin-mediated shrinkage of the Synechocystis cells plays an important role in changes of gene expression in response to hyperosmotic stress.
Shapiguzov
Alexey
A
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
Lyukevich
Alexander A
AA
Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan.|Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
Allakhverdiev
Suleyman I
SI
Institute of Basic Biological Problems RAS, Pushchino, Moscow Region 142292, Russia.|Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan.
Sergeyenko
Tatiana V
TV
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
Suzuki
Iwane
I
Department of Molecular Biomechanics, School of Life Science, The Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan.|Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan.
Murata
Norio
N
Department of Molecular Biomechanics, School of Life Science, The Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan.|Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan.
Los
Dmitry A
DA
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014867:Water / Q000502:physiology*
D020346:Aquaporins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D014867:Water