[Correlation of mRNA expression of hypoxia inducible factor-1alpha to biological features of pancreatic cancer].
OBJECTIVE
Hypoxia inducible factor-1alpha(HIF-1alpha) is universally expressed in many types of cells, and plays a key role in modulation of tumor related genes under hypoxia. Our research was to detect mRNA expression of HIF-1alpha,and explore its relationship with biological characteristics of pancreatic cancer, such as tumor angiogenesis, tumor cell proliferation, differentiation, and metastasis.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect mRNA expression of HIF-1alpha in 50 specimens of pancreatic cancer tissues and relevant adjacent tissues. Expressions of Survivin, proliferating cell nuclear antigen (PCNA), and CD34 were measured by SP immunohistochemistry. Spearman rank correlation was used to analyze the correlation of mRNA expression of HIF-1alpha to expressions of Survivin, PCNA, and CD34.


RESULTS
mRNA level of HIF-1alpha in pancreatic cancer tissues was significantly higher than that in adjacent tissues (0.77+/-0.04 vs. 0.56+/-0.01, P < 0.01). No significant difference in mRNA expression of HIF-1alpha was observed among tumor tissues of different pathologic grades (P > 0.05). mRNA level of HIF-1alpha in pancreatic cancer tissues of stages I-II was significantly higher than that in cancer tissues of stages III-IV (0.79+/-0.05 vs. 0.76+/-0.04, P < 0.05). mRNA level of HIF-1alpha was correlated with expressions of Survivin, PCNA, and CD34 (r=1.00, 0.91, and 0.68, respectively, P < 0.01).


CONCLUSIONS
mRNA expression of HIF-1alpha has significant correlations with tumor angiogenesis, cell proliferation, apoptosis, and metastasis of pancreatic cancer.
Wei
Hai-Yan
HY
Pancreatic Center of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, P. R. China. cherrywei640@163.com
Chen
Li-Bo
LB
Wang
Chun-You
CY
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism*; D017209:Apoptosis; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis* / Q000235:genetics; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077022:Survivin
D018952:Antigens, CD34; C107489:BIRC5 protein, human; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D000077022:Survivin