Thioredoxin induction of peripheral blood mononuclear cells in mice in response to a single bout of swimming exercise.
Thioredoxin (TRX) is a stress-inducible protein with diverse intracellular functions, which is expressed under conditions of oxidative stress. Exercise is known to cause oxidative stress by the generation of oxygen radicals from various biological pathways. The purpose of this study was to determine the level of TRX induction of cellular extracts prepared from peripheral blood mononuclear cells after a 30-min swimming exercise in mice. Plasma corticosterone concentration, considered to be a marker for exercise-induced various stress, rose significantly (p < 0.05) 0.5 h after exercise and rapidly dropped down following recovery. The carbonyl proteins as a marker of oxidative stress were significantly (p < 0.05) higher after 6 and 12 h of recovery in cytosolic extracts. The cytoplasm and nucleus TRX expressions were slightly higher to resting values after 12 and 24 h of recovery. The nucleus TRX expression was significantly (p < 0.05) higher after 24 h of recovery. These findings demonstrate that exercise-induced oxidative stress may be associated with increased intracellular TRX expression after 12 and/or 24 h after exercise in peripheral blood mononuclear cells. It is implied that this delayed and prolonged over-expression of TRX may play some roles in response to exercise-induced oxidative stress.
Sumida
S
S
Laboratory of Exercise Physiology and Biochemistry, Osaka Gakuin University, Suita, Osaka, Japan. sumida@utc.osaka-gu.ac.jp
Nakamura
H
H
Yodoi
J
J
D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000097:blood*; D005080:Exercise Test; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008571:Men; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology*; D013550:Swimming / Q000502:physiology*; D013879:Thioredoxins / Q000097:blood*
D013879:Thioredoxins; D003345:Corticosterone