title: Effect of ingested concentrate and components of sake on epidermal permeability barrier disruption by UVB irradiation.
abstract: Daily topical applications of the concentrate of sake (CS) have been shown to reduce epidermal barrier disruption in murine skin caused by ultraviolet B (UVB) radiation, while one of the components of sake, ethyl alpha-D-glucoside (alpha-EG), also reduces barrier disruption. We confirmed the effect of oral ingestion of various doses of CS on epidermal barrier disruption caused by UVB irradiation in hairless mice. Then, to identify the effective components, we quantitatively analyzed alpha-EG, organic acids, and glycerol, the main components of CS, and examined the effect of various concentration of each on barrier disruption. alpha-EG and organic acids showed comparable results to CS itself, and transepidermal water loss levels in murine skin were significantly decreased as compared with the control. Furthermore, an investigation of the dose dependency of these agents was performed and the results showed the significant effectiveness of alpha-EG. In addition, red wine concentrate (WC) and beer concentrate (BC) were examined in order to confirm the unique effects of CS. Similar effects were not found with WC and BC.
authors.affiliation: General Research Laboratory, Ozeki Co., Nishinomiya, Hyogo 663-8227, Japan. masato.hirotsune@ozeki.co.jp
authors.forename: Masato
authors.initials: M
authors.lastname: Hirotsune
authors.forename: Akinori
authors.initials: A
authors.lastname: Haratake
authors.forename: Aya
authors.initials: A
authors.lastname: Komiya
authors.forename: Jun
authors.initials: J
authors.lastname: Sugita
authors.forename: Toru
authors.initials: T
authors.lastname: Tachihara
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Komai
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Hizume
authors.forename: Kenji
authors.initials: K
authors.lastname: Ozeki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ikemoto
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis; D000818:Animals; D001515:Beer / Q000032:analysis; D004817:Epidermis / Q000502:physiology / Q000528:radiation effects*; D005285:Fermentation; D051379:Mice; D008812:Mice, Hairless; D012275:Oryza; D010539:Permeability; D014466:Ultraviolet Rays; D014870:Water Loss, Insensible; D014920:Wine / Q000032:analysis