title: Repeated open application test with methyldibromo glutaronitrile, a multicentre study within the EECDRG.
abstract: Contact allergy to and allergic contact dermatitis from methyldibromo glutaronitrile (MDBGN) have frequently been reported. This study was initiated to help determine the optimal patch test preparation for MDBGN. In 51 patients with a doubtful or a positive patch test reaction to at least 1 of 4 test preparations with MDBGN in petrolatum at 1.0% w/w, 0.5%, 0.3% and 0.1%, a repeated open application test (ROAT) with moisturizers with and without MDBGN at 0.03% w/w was performed on the upper arms for 2 weeks. 18 of the 51 (35.3%) patients developed a positive ROAT. In all patients, there was a positive ROAT only to the moisturizer with MDBGN (P < 0.001). A statistically significant association was also found between the patch test reactivity (PTRL) and the outcome of the ROAT (P < 0.001). If only considering those with a PTRL above 0.3%, thus with negative or doubtful test reactions to 0.1% and 0.3%, there were still statistically significantly more patients with a positive ROAT to the moisturizer with MDBGN than to the moisturizer without MDBGN. The study demonstrates that patch testing with MDBGN at 0.3% and 0.1% will miss clinically relevant patch test reactions to MDBGN.
authors.affiliation: Department of Occupational and Environmental Dermatology, Malmö University Hospital, S-205 02 Malmö, Sweden. birgitta.gruvberger@derm.mas.lu.se
authors.forename: B
authors.initials: B
authors.lastname: Gruvberger
authors.forename: K E
authors.initials: KE
authors.lastname: Andersen
authors.forename: F M
authors.initials: FM
authors.lastname: Brandão
authors.forename: D P
authors.initials: DP
authors.lastname: Bruynzeel
authors.forename: M
authors.initials: M
authors.lastname: Bruze
authors.forename: P J
authors.initials: PJ
authors.lastname: Frosch
authors.forename: A
authors.initials: A
authors.lastname: Goossens
authors.forename: A
authors.initials: A
authors.lastname: Lahti
authors.forename: H I
authors.initials: HI
authors.lastname: Maibach
authors.forename: T
authors.initials: T
authors.lastname: Menné
authors.forename: D
authors.initials: D
authors.lastname: Orton
authors.forename: S
authors.initials: S
authors.lastname: Seidenari
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000009:adverse effects*; D003358:Cosmetics / Q000009:adverse effects; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis* / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D008452:Maximum Allowable Concentration; D008875:Middle Aged; D009570:Nitriles / Q000009:adverse effects*; D010328:Patch Tests / Q000379:methods* / Q000592:standards*; D011310:Preservatives, Pharmaceutical / Q000009:adverse effects*; D012107:Research Design; D013997:Time Factors
chemical_list: D000485:Allergens; D003358:Cosmetics; D009570:Nitriles; D011310:Preservatives, Pharmaceutical; C039411:1,2-dibromo-2,4-dicyanobutane