title: [Expressions of cyclins A, B1, D3, and E in asynchronous and synchronous MOLT-4 cells of G1 phase].
abstract: OBJECTIVE
  We have testified expression imbalance of Cyclins A, B1, D3, and E in MOLT-4 cells, which were synchronized by "double thymidine blocks", with flow cytometry (FCM). However, comparisons of Cyclins expressions between synchronized cells and asynchronized cells haven't been performed at that time because of technique limitations. This study was to compare expressions of Cyclins A, B1, D3, and E in G(1) phase of asynchronized and synchronized cells by newly established "postsorting Western blot", and to conform the unreasonableness of using double thymidine blocks to synchronize cells to analyze normal cell cycles.
  
  
  METHODS
  MOLT-4 cells were synchronized at G(1) phase by double thymidine blocks, asynchronous cells of G(1) phase were sorted by FCM. Western blot and double parameters analysis of DNA/Cyclins were performed to detect Cyclins A, B1, D3, and E expressions in asynchronous and synchronous MOLT-4 cells of G(1) phase.
  
  
  RESULTS
  There were almost no expressions of Cyclins A, B1 in asynchronous MOLT-4 cells of G(1) phase, and obvious expressions in synchronized cells of G(1) phase. Expressions of Cyclins D3, E in synchronous MOLT-4 cells of G(1) phase were higher than those in asynchronous cells of G(1) phase. The FCM results were accordant with Western blot results.
  
  
  CONCLUSIONS
  The expressions of Cyclins in synchronized cells obtained through "double thymidine blocks" can't represent their expressions in normal cells. Thus, synchronous cells produced by "double thymidine blocks" are not ideal experimental models for analyzing normal cell cycles.
authors.affiliation: Department of General Surgery/ Cancer Research Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei, 430030, P. R. China.
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Yu
authors.forename: Yong-Dong
authors.initials: YD
authors.lastname: Feng
authors.forename: De-Ding
authors.initials: DD
authors.lastname: Tao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Wu
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Gong
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D019925:Cyclin A / Q000378:metabolism; D019926:Cyclin B / Q000378:metabolism; D056744:Cyclin B1; D056743:Cyclin D3; D019927:Cyclin E / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism*; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology; D013936:Thymidine / Q000494:pharmacology
chemical_list: C534699:CCNB1 protein, human; C534696:CCND3 protein, human; D019925:Cyclin A; D019926:Cyclin B; D056744:Cyclin B1; D056743:Cyclin D3; D019927:Cyclin E; D016213:Cyclins; D013936:Thymidine