[Construction of fast propagation system of Houttuynia cordata new line].
OBJECTIVE
To construct the fast propagation system of Houttuynia cordata Thunb. new line by selecting the proper type and concentration of plant growth regulators and proper explants.


METHODS
The different explants of Houttuynia cordata new line were cultured on MS media with different concentration of different plant grow regulators.


RESULTS
Stem node over-ground and shoot tip were optimum explants for fast propagation. The stem which node grew on MS medium with KT at 0.5 mg x L(-1) could induce more leaves and grow higher. The shoot tip cultured on MS medium with 1.0 mg x L(-1) 6-BA could induce better callus. Adventitious shoots were better achieved from over-ground nodal explants cultured on MS medium supplemented with 6-BA at 1.0 mg x L(-1) and NAA at 0.2 mg x L(-1), or 6-BA at 0.5 mg x L(-1) and NAA at 0.2 mg x L(-1). MS medium with IAA at 0.2 mg x L(-1) was the best one on inducing roots.


CONCLUSIONS
The propagat coefficient can be highly improved by inducing adventitious shoots through stem node over-ground, and thus plentiful seeds for production can be provided.
Wu
Wei
W
Agronomy College, Sichuan Agricultural University, Yaan 625014, China.
Zheng
You-liang
YL
Liu
Fan
F
Tan
Gang
G
Ren
Hong-yu
HY
Zhang
Wen-cai
WC
D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D032134:Saururaceae / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators