Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo.
Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.
Hamada
Yoshinosuke
Y
Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan. y-hamada@sahs.med.osaka-u.ac.jp
Yuki
Kanako
K
Okazaki
Masayuki
M
Fujitani
Wataru
W
Matsumoto
Takuya
T
Hashida
Michiyo Kobashi
MK
Harutsugu
Kentaro
K
Nokihara
Kiyoshi
K
Daito
Michiharu
M
Matsuura
Nariaki
N
Takahashi
Junzo
J
D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D043925:Angiogenesis Inducing Agents / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000737:chemistry / Q000494:pharmacology* / Q000502:physiology; D014443:Tyrosine / Q000502:physiology
D043925:Angiogenesis Inducing Agents; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D014443:Tyrosine