title: Quality control of roots of Eleutherococcus senticosus by HPLC.
abstract: An HPLC method based on several known methods for the determination of eleutherosides B and E was developed, optimised and validated in terms of linearity, precision (repeatability and intermediate precision on different days and at different concentration levels) and accuracy (recovery). The extraction procedure, the extraction solvent and the extraction yield were evaluated and optimised. A reversed-phase RP-18 column gradient eluted with a two-phase system consisting of phosphoric acid:water (0.5:99.5) and acetonitrile was used to evaluate the samples; detection was at 220 nm. Although eleutherosides B and E are commercially available, they are very costly, and therefore ferulic acid was chosen as external standard. The correction factors for the response of ferulic acid against both eleutherosides were determined and validated. This method, accepted by the European Pharmacopoeia Commission, will be included in the monograph on Eleutherococcus senticosus roots to assay the content of eleutherosides B and E.
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium. Sandra.Apers@ua.ac.be
authors.forename: Sandra
authors.initials: S
authors.lastname: Apers
authors.forename: Tania
authors.initials: T
authors.lastname: Naessens
authors.forename: Sabine
authors.initials: S
authors.lastname: Van Miert
authors.forename: Luc
authors.initials: L
authors.lastname: Pieters
authors.forename: Arnold
authors.initials: A
authors.lastname: Vlietinck
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028001:Eleutherococcus / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D017705:Lignans; D015394:Molecular Structure; D010666:Phenylpropionates / Q000737:chemistry; D028321:Plant Preparations / Q000592:standards; D018517:Plant Roots / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005960:Glucosides; D017705:Lignans; D010666:Phenylpropionates; D028321:Plant Preparations; C421885:eleutheroside E; C028305:syringin