title: A simple, efficient, and economical method for recovering DNA from agarose gel.
abstract: A simple method of recovering DNA from agarose gel that is fast, inexpensive, and friendly both to operators and environment is described. Two rows of wells are made in an agarose gel, and a DNA sample is loaded into the well nearest to the negative pole for separation by electrophoresis. Recovery is accomplished by pipetting the DNA-containing TAE buffer from the well near the positive pole after target DNA fragments have migrated into the well. A recovery rate of up to 94 +/- 2.3% was observed with this method.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, PR China. fanchang@sohu.com
authors.forename: Chang-Fa
authors.initials: CF
authors.lastname: Fan
authors.forename: Xing-Guo
authors.initials: XG
authors.lastname: Mei
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005782:Gels / Q000032:analysis* / Q000737:chemistry*; D012685:Sepharose / Q000032:analysis* / Q000737:chemistry*; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D005782:Gels; D004247:DNA; D012685:Sepharose