[Epidemiologic markers in Haemophilus influenzae].
Twenty-six strains of Haemophilus influenzae, isolated from diverse pathological products in two different hospitals in the center of Tunisia (Sousse-Monastir) have been compared with two different genotypic techniques: AP-PCR and pulsed-field gel electrophoresis. These two techniques showed a high discriminating power. The combination of the results of both techniques were complementary and have individualized twenty-five heterogeneous patterns among the twenty-six strains. Among the strains of respiratory origin, only two were identical, they have been isolated from two patients hospitalized in the same period and the same hospital. Excepted the two above mentioned cases, this study showed a high genetical heterogeneity of the strains.
Oueslati
S
S
Laboratoire des maladies transmissibles et des substances biologiquement actives, (Lab.MDT-01), faculté de pharmacie, 5000 Monastir, Tunisia. oueslati1@yahoo.fr
Mzoughi
R
R
Bouallegue
O
O
Aouni
M
M
D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006192:Haemophilus Infections / Q000453:epidemiology*; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014416:Tunisia / Q000453:epidemiology