Use of the restriction enzyme EcoRI for pulsed-field gel electrophoretic analysis of Helicobacter pylori.
Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (typeability was 97%).
Hosaka
Yoshio
Y
Department of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa 228, Japan.
Irinoda
Kazuhiko
K
Nakano
Ryuichi
R
Tanabe
Satosi
S
Koizumi
Wasaburo
W
Saigenji
Katunori
K
Inoue
Matsuhisa
M
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D016521:Electrophoresis, Gel, Pulsed-Field; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D015183:Restriction Mapping
D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI