Characterization of cytolethal distending toxin of campylobacter species isolated from captive macaque monkeys.
An association between certain Campylobacter species and enterocolitis in humans and nonhuman primates is well established, but the association between cytolethal distending toxin and disease is incompletely understood. The purpose of the present study was to examine Campylobacter species isolated from captive conventionally raised macaque monkeys for the presence of the cdtB gene and for cytolethal distending toxin activity. The identity of each isolate was confirmed on the basis of phenotypic and genotypic analyses. The presence of cytolethal distending toxin was confirmed on the basis of characteristic morphological changes in HeLa cells incubated with filter-sterilized whole-cell lysates of reference and monkey Campylobacter isolates and examinations by light microscopy, confocal microscopy, and flow cytometry. Although cdtB gene sequences were found in both Campylobacter jejuni and Campylobacter coli, the production of cytolethal distending toxin correlated positively (P < 0.0001) only with C. jejuni. We concluded that cytolethal distending toxin activity is a characteristic of C. jejuni. Our C. jejuni cdtB gene-specific PCR assay might be of assistance for differentiating toxigenic C. jejuni from C. coli in clinical laboratories.
Dassanayake
Rohana P
RP
Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
Zhou
You
Y
Hinkley
Susanne
S
Stryker
Cynthia J
CJ
Plauche
Gail
G
Borda
Juan T
JT
Sestak
Karol
K
Duhamel
Gerald E
GE
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D002169:Campylobacter Infections / Q000382:microbiology; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D003967:Diarrhea / Q000382:microbiology*; D005838:Genotype; D006367:HeLa Cells; D006801:Humans; D008251:Macaca / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D017422:Sequence Analysis, DNA
D001427:Bacterial Toxins; C067044:cytolethal distending toxin