[Extraction of coumarins from Angelica dahurica by supercritical extraction and GC-MS analysis].
OBJECTIVE
To extract coumarins from Angelica Dahurica by supercritical CO2.


METHODS
Six-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. Constituents of the coumarins were analyzed by GC-MS, and their relative contents were determined by normalization method of areas.


RESULTS
Total 15 coumarins were separated, the relative contents of oxyimperatorin, imperatorin and isoimperatorin being up to 42.40%, 22.14% and 12.12% respectively. Optimum extraction process was established: 21 MPa as extraction pressure, 50 degrees C as extraction temperature, 3 h as time for extraction, 20 mesh as pulverized degree, 6.5 MPa as separation pressure and 30 degrees C as separation temperature.


CONCLUSIONS
Changes in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably.
Liu
Hong-mei
HM
Biochemical Engineering College of Beijing Union University, Beijing 100023, China. hgthong-mei@buu.com.cn
Zhang
Ming-xian
MX
D029969:Angelica / Q000737:chemistry*; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification*; D011564:Furocoumarins / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D010946:Plants, Medicinal / Q000737:chemistry*
D003374:Coumarins; D011564:Furocoumarins; C055542:isoimperatorin; D002245:Carbon Dioxide; C031534:imperatorin