title: Mapping segmental and sequence variations among laboratory mice using BAC array CGH.
abstract: We used arrays of 2069 BACs (1303 nonredundant autosomal clones) to map sequence variation among Mus spretus (SPRET/Ei and SPRET/Glasgow) and Mus musculus (C3H/HeJ, BALB/cJ, 129/J, DBA/2J, NIH, FVB/N, and C57BL/6) strains. We identified 80 clones representing 74 autosomal loci of copy number variation (|log(2)ratio| >/= 0.4). These variant loci distinguish laboratory strains. By FISH mapping, we determined that 63 BACs mapped to a single site on C57BL/6J chromosomes, while 17 clones mapped to multiple chromosomes (n = 16) or multiple sites on one chromosome (n = 1). We also show that small ratio changes (Delta log(2)ratio approximately 0.1) distinguish homozygous and heterozygous regions of the genome in interspecific backcross mice, providing an efficient method for genotyping progeny of backcrosses.
authors.affiliation: Cancer Research Institute, University of California San Francisco, San Francisco, California 94143, USA.
authors.forename: Antoine M
authors.initials: AM
authors.lastname: Snijders
authors.forename: Norma J
authors.initials: NJ
authors.lastname: Nowak
authors.forename: Bing
authors.initials: B
authors.lastname: Huey
authors.forename: Jane
authors.initials: J
authors.lastname: Fridlyand
authors.forename: Sindy
authors.initials: S
authors.lastname: Law
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Conroy
authors.forename: Taku
authors.initials: T
authors.lastname: Tokuyasu
authors.forename: Kubilay
authors.initials: K
authors.lastname: Demir
authors.forename: Readman
authors.initials: R
authors.lastname: Chiu
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Mao
authors.forename: Ajay N
authors.initials: AN
authors.lastname: Jain
authors.forename: Steven J M
authors.initials: SJ
authors.lastname: Jones
authors.forename: Allan
authors.initials: A
authors.lastname: Balmain
authors.forename: Daniel
authors.initials: D
authors.lastname: Pinkel
authors.forename: Donna G
authors.initials: DG
authors.lastname: Albertson
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003433:Crosses, Genetic; D018628:Gene Dosage; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D016678:Genome; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013045:Species Specificity
chemical_list: D005819:Genetic Markers