Bioprocessing strategies to improve heterologous protein production in filamentous fungal fermentations.
Filamentous fungi have long been used for the production of metabolites and enzymes. With developments in genetic engineering and molecular biology, filamentous fungi have also achieved increased attention as hosts for recombinant DNA. However, the production levels of non-fungal proteins are usually low. Despite the achievements obtained using molecular tools, the heterologous protein loss caused by extracellular fungal protease degradation persists. This review provides an overview of the potential bioprocessing strategies that can be applied to inhibit protease activity thereby enhancing heterologous protein production.
Wang
Liping
L
Department of Chemical Engineering, Ohio University, Athens, OH 45701, USA.
Ridgway
Darin
D
Gu
Tingyue
T
Moo-Young
Murray
M
D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005658:Fungi / Q000235:genetics* / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D005656:Fungal Proteins; D011994:Recombinant Proteins; D010447:Peptide Hydrolases