The MICAL proteins and rab1: a possible link to the cytoskeleton?
The small GTPase rab1 plays a role in vesicle trafficking between ER and the Golgi complex. Recently, MICAL-1 was identified as new rab1 interacting protein. In this study, we show an interaction between two additional members of the MICAL family and rab1 in a yeast two-hybrid approach and GST pulldown experiments. We present data about the previously uncharacterized MICAL-3 concerning tissue distribution, size, and cellular localization. Furthermore, we investigated the connection between MICAL proteins and the cytoskeleton. Using the microtubule depolymerizing drug nocodazole we detected a link between MICAL-1 and -3, and the microtubule cytoskeleton.
Fischer
Julia
J
Department of Experimental Tumorbiology, University of Muenster, Badestr. 9, D-48149 Muenster, Germany.
Weide
Thomas
T
Barnekow
Angelika
A
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D008840:Microfilament Proteins; D006899:Mixed Function Oxygenases; D009928:Organ Specificity; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D014018:Tissue Distribution; D020761:rab1 GTP-Binding Proteins / Q000378:metabolism*; D000096985:Calponins
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D008840:Microfilament Proteins; D006899:Mixed Function Oxygenases; D020761:rab1 GTP-Binding Proteins; C455272:MICAL1 protein, human; D000096985:Calponins