[Effects of Astragalus membranaceus injection on nitric oxide and endothelin concentration of intestinal mucosa after hemorrhage shock-reperfusion in rats].
OBJECTIVE
To observe the effects of Stragalus membranaceus injection on nitric oxide and endothelin levels of intestinal mucosa in reperfusion injury after hemorrhage shock.


METHODS
32 SD rats were randomly divided into four groups: normal group, model group, low dosage group, (treated with Astragalus membranaceus 10 g x kg(-1)); high dosage group (treated with Astragalus membranaceus 20 g x kg(-1)). Models of hemorrhagic shock for 60 minutes and reperfusion for 90 minutes were created. The animals were administrated 3 mL therapeutic solution before reperfusion. At the end of study, intestinal pathology was observed, and the concentration of lactic acid (LD), nitric oxide (NO), endothelin (ET) of intestinal mucosa were detected.


RESULTS
The intestinal pathology showed that intestinal mucosa epithelial cells damage in model group was severe, in low dosage group was medium, in high dosage group was slight, and no obvious damage was found in normal group. The concentration of LD and NO of small intestine mucous membrane in model group and low dosage group were significantly higher than those in high dosage group and normal group (P < 0.05), but there were no significant differences between high dosage group and normal group (P > 0.05). The concentration of ET of small intestine mucous membrane in model group was the highest of the four groups (P < 0.05). The concentration of ET in low dosage group was significantly higher than that in high dosage group and normal group (P < 0.05), but there were no significant differences between high dosage group and normal group (P > 0.05).


CONCLUSIONS
Stragalus membranaceus injection can reduce small intestine mucous damage by protecting endothelium function in injury after hemorrhage shock-reperfusion.
Hei
Zi-qing
ZQ
Department of Anesthesiology, The Third Affiliated Hospital of Zhong Shan University, Guangzhou 510630, China. heiziqing@sina.com
Zhang
Jin-jun
JJ
Lin
Shi-qing
SQ
Liu
Ke-xuan
KX
Chen
Bing-xue
BX
D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D016232:Endothelins / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism / Q000473:pathology; D007275:Injections, Intravenous; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D012771:Shock, Hemorrhagic / Q000150:complications
D004365:Drugs, Chinese Herbal; D016232:Endothelins; D009569:Nitric Oxide; D019344:Lactic Acid