title: Effect of lung hypoplasia on birth-related changes in the pulmonary circulation in sheep.
abstract: Lung hypoplasia (LH) is a serious cause of neonatal compromise, but little is known of its functional effects on the pulmonary circulation. Our aim was to characterize birth-related changes in the pulmonary circulation of newborn lambs with LH and to compare them with alterations in respiratory function. LH was induced in six ovine fetuses by the creation of a tracheo-amniotic shunt as well as amniotic fluid drainage starting at 105.6+/-1.5 (mean+/-SEM) days of gestation (term approximately 147 d). At 139.9+/-0.3 d, fetuses were exteriorized under anesthesia to implant vascular catheters and an ultrasonic flow probe around the left pulmonary artery. The lambs then were delivered and ventilated for 2 h, during which systemic and pulmonary artery pressures, left pulmonary blood flow, and measures of respiratory function were recorded. At autopsy, lungs were weighed and volume was measured at 20 cm H2O. In LH lambs, lung weight was 25% lower and respiratory system compliance was 30% lower than in controls. Mean pulmonary blood flow in LH lambs was 42% lower and pulmonary vascular resistance was 138% higher than in controls. Morphometry showed that volume density of pulmonary arteries in LH was 30% lower than in controls. We conclude that, in this LH model, changes in ventilatory indices were proportional to the change in lung size, whereas changes in the pulmonary circulation were greater than the change in lung size and were associated with reduced density of pulmonary arteries. LH severely impairs normal adaptation of the pulmonary circulation in the perinatal period.
authors.affiliation: Department of Physiology, Monash University, Clayton, Victoria, Australia. dks@med.monash.edu.au
authors.forename: Keiji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Stuart B
authors.initials: SB
authors.lastname: Hooper
authors.forename: Megan L
authors.initials: ML
authors.lastname: Cock
authors.forename: Richard
authors.initials: R
authors.lastname: Harding
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005260:Female; D005865:Gestational Age; D008168:Lung / Q000033:anatomy & histology / Q000098:blood supply* / Q000473:pathology* / Q000502:physiology; D008170:Lung Compliance; D011247:Pregnancy; D011651:Pulmonary Artery / Q000033:anatomy & histology; D011652:Pulmonary Circulation / Q000502:physiology*; D012129:Respiratory Function Tests; D012143:Respiratory Physiological Phenomena; D034561:Sheep, Domestic