Pineapple juice for digestion of swamp eel viscera for harvesting infective-stage larva of Gnathostoma spp.
Third-stage larvae were used as antigen in the diagnosis of gnathostomiasis in Western blot analysis. Normally, the larvae were obtained from digestion of eel's liver (Fluta alba) by the enzyme pepsin. We used pineapple juice (Ananus comosus) instead of enzyme pepsin in harvesting Gnathostoma spinigerum third-stage larvae. The difference in recovered larvae numbers, between pineapple juice and pepsin, were not statistically significantly different (p>0.05). The larvae from pepsin and pineapple juice digestion were cultivated on BME for 7 days; the survival rates were not significantly different (p>0.05). Thus, pineapple juice is another enzyme of choice for recovering Gnathostoma spinigerum third-stage larvae.
Soogarun
Suphan
S
Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Suwansaksri
Jamsai
J
Wiwanitkit
Viroj
V
D031230:Ananas / Q000378:metabolism; D000818:Animals; D000947:Antigens, Helminth; D001628:Beverages; D015153:Blotting, Western; D004063:Digestion / Q000502:physiology*; D004524:Eels / Q000469:parasitology*; D005393:Fish Diseases / Q000175:diagnosis / Q000469:parasitology*; D016266:Food Parasitology; D006039:Gnathostoma / Q000276:immunology* / Q000302:isolation & purification; D007814:Larva / Q000276:immunology / Q000469:parasitology; D007816:Larva Migrans, Visceral; D008099:Liver / Q000469:parasitology; D010434:Pepsin A / Q000378:metabolism; D012996:Solutions; D017205:Spirurida Infections / Q000175:diagnosis*
D000947:Antigens, Helminth; D012996:Solutions; D010434:Pepsin A