[Effects of dermal template on the biological behaviors of fibroblasts during wound healing].
OBJECTIVE
To study the effects of dermal template on the biological behaviors of fibroblasts during wound healing.


METHODS
A total of 120 rats were made full-thickness wound modes on the dorsum and divided into 4 groups, in group 1, the wounds were allowed to heal by contraction(ConT); in group 2, the wounds covered with full-thickness skin grafts (FTSG); in group 3, the wounds were with split thickness skin grafts (STSG); and in group 4, the wounds were covered by dermal regeneration template with overlying thin split-thickness autograft (ADMT). The specimens were obtained at one week, two weeks, four weeks, six weeks, and twelve weeks respectively. The expressions of alpha smooth muscle actin(alpha-SMA, characteristic of MFB), fibronectin (FN), integrin alpha2 beta1 and transforming growth factor beta1 (TGF-beta1) were examined by immunohistochemical analysis.


RESULTS
Positive expression of alpha-SMA, FN, integrin alpha2 beta1 and TGF-beta1 in ADMT groups was significantly lower than that in STSG group and ConT group, but higher than that in FTSG group (P < 0.05).


CONCLUSIONS
Dermal regeneration template can inhibit the transformation of FB to MFB and restrain the expression of FN, integrin alpha2 beta1, and TGF-beta1 in fibroblasts which might reduce the possibility of hypertrophic scarring, and improve wound healing.
Liu
Yingkai
Y
Department of Burns, Burn Institute of Shanghai, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, P R China.
Lu
Shuliang
S
Qing
Chun
C
Liao
Zhenjiang
Z
Shi
Jixiang
J
D000199:Actins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D020405:Dermis / Q000293:injuries / Q000378:metabolism / Q000601:surgery*; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D038982:Integrin alpha2beta1 / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016038:Skin Transplantation / Q000379:methods*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing
D000199:Actins; D005353:Fibronectins; D038982:Integrin alpha2beta1; D053773:Transforming Growth Factor beta1