title: Expression of the cysteinyl leukotriene receptors cysLT(1) and cysLT(2) in aspirin-sensitive and aspirin-tolerant chronic rhinosinusitis.
abstract: BACKGROUND
  Cysteinyl leukotrienes play a disease-regulating role in rhinosinusitis and asthma, particularly aspirin-sensitive disease. They act through 2 G-protein coupled receptors termed cysteinyl leukotriene type 1 receptor (cysLT 1 ) and cysteinyl leukotriene type 2 receptor (cysLT 2 ). We previously compared expression of cysLT 1 on mucosal leukocytes in patients with aspirin-sensitive and aspirin-tolerant rhinosinusitis.
  
  
  OBJECTIVE
  To compare expression of cysLT 1 and cysLT 2 on leukocytes, mucus glands, and epithelium in 32 patients with chronic polypoid rhinosinusitis (21 aspirin-sensitive, 11 aspirin-tolerant) and 9 normal controls.
  
  
  METHODS
  Total numbers of CD45 + leukocytes, percentages of these cells expressing cysLT 1 or cysLT 2 , and percentages of the total epithelial and glandular areas expressing cysLT 1 or cysLT 2 were measured in sections of nasal biopsies by using immunohistochemistry and image analysis.
  
  
  RESULTS
  The percentages of mucosal CD45 + leukocytes expressing cysLT 1 were significantly ( P < .0001) elevated in the aspirin-sensitive but not the aspirin-tolerant patients compared with the controls. In contrast, the percentages of leukocytes expressing cysLT 2 did not differ significantly in the 3 groups. On epithelial and glandular cells, expression of cysLT 2 significantly exceeded that of cysLT 1 in both the patients with rhinosinusitis and the controls ( P < or = .004), although there was no significant difference in the expression of either receptor in the patients with rhinosinusitis (aspirin-sensitive or aspirin-tolerant) and the controls.
  
  
  CONCLUSIONS
  Although cysLT 1 expression predominates on inflammatory leukocytes in patients with aspirin-sensitive rhinosinusitis, the effects of cysteinyl leukotrienes on glands and epithelium may be mediated predominantly through cysLT 2. This has potentially important therapeutic implications.
authors.affiliation: Department of Asthma, Allergy and Respiratory Science, Guy's Hospital, London, UK. chris.corrigan@kcl.ac.uk
authors.forename: Chris
authors.initials: C
authors.lastname: Corrigan
authors.forename: Kirsty
authors.initials: K
authors.lastname: Mallett
authors.forename: Sun
authors.initials: S
authors.lastname: Ying
authors.forename: David
authors.initials: D
authors.lastname: Roberts
authors.forename: Abhi
authors.initials: A
authors.lastname: Parikh
authors.forename: Glenis
authors.initials: G
authors.lastname: Scadding
authors.forename: Tak
authors.initials: T
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D001241:Aspirin / Q000009:adverse effects*; D002908:Chronic Disease; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D018077:Receptors, Leukotriene / Q000378:metabolism*; D012220:Rhinitis / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012852:Sinusitis / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D008565:Membrane Proteins; D018077:Receptors, Leukotriene; C412375:cysteinyl leukotriene receptor 2; C083169:leukotriene D4 receptor; D001241:Aspirin