Strongyloides ratti: thermokinesis of glycolytic enzyme- and lectin-treated third-stage infective larvae in vitro.
The infective third-stage larvae (L3s) of a parasitic nematode of rodents, Strongyloides ratti, showed three types of thermokinesis on a temperature gradient using an in vitro agarose tracking assay method. These depended both on the pattern of gradient temperature and the prior culture temperature. Most L3s (> or = 80%) isolated from rat feces cultured at 25 degrees C and placed on a gradient at temperatures between 30 degrees C and 37 degrees C showed no directional response, at 22-29 degrees C more than 50% of the L3s showed positive thermokinesis, at 21 degrees C L3s showed positive, negative and no directional responses in the same ratio, while at 18-20 degrees C, L3s showed negative thermokinesis (approx. 40%) or no directional response (approx. 60%) as in our previous study. The present study describes the effects of glycolytic enzyme- and lectin-treated positive thermokinesis of L3s. alpha-Glucosidase or concanavalin A significantly exhibited inhibitory effects on thermokinesis.
Tobata-Kudo
Hiroe
H
Tobata Laboratory, 1-20-10 Asakawagakuendai, Yahatanishi-ku, 807-0871 Kitakyushu, Japan. hiroemushi@ybb.ne.jp
Kudo
Hideaki
H
Tada
Isao
I
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D004798:Enzymes / Q000494:pharmacology*; D006019:Glycolysis; D007814:Larva / Q000187:drug effects / Q000502:physiology; D037102:Lectins / Q000494:pharmacology*; D009068:Movement / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017170:Strongyloides ratti / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D013696:Temperature; D000520:alpha-Glucosidases / Q000494:pharmacology
D004798:Enzymes; D037102:Lectins; D003208:Concanavalin A; D000520:alpha-Glucosidases