Cloning and expression of human lectin-like oxidized low density lipoprotein receptor-1 in Pichia pastoris.
Lectin-like oxidatively-modified LDL receptor-1 (LOX-1) is a major receptor for oxidized low-density lipoprotein (oxLDL) in aortic endothelial cells. Human LOX-1 (hLOX-1) gene (cDNA) was cloned from the monocytic leukemic cell line THP-1 and expressed in Pichia pastoris. The recombinant protein (rhLOX-1) was purified by his-tag affinity chromatography. Preliminary identification was performed by Western blot analysis and a ligand-receptor binding assay showed that the protein had specific oxLDL-binding activity.
Huang
Zhentai
Z
Department of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences & Peking Union Medical College, 100050, Beijing, P.R. China.
Zhang
Tiantai
T
Yang
Jinling
J
Zhu
Ping
P
Du
Guanhua
G
Cheng
Kedi
K
D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010843:Pichia / Q000235:genetics*; D010957:Plasmids; D011973:Receptors, LDL / Q000737:chemistry / Q000235:genetics*; D051131:Receptors, Oxidized LDL; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D051127:Scavenger Receptors, Class E
D017931:DNA Primers; C495137:OLR1 protein, human; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; D011994:Recombinant Proteins; D051127:Scavenger Receptors, Class E