title: [FK506 promotes apoptosis of macrophages activated by homogenate of allogenic nerve].
abstract: OBJECTIVE
  To study the effect of FK506 in promoting apoptosis of peripheral blood-derived macrophages activated by homogenate of allogenic nerve tissues.
  
  
  METHODS
  Homogenate of the allogenic nerve tissues was prepared using the sciatic nerve and injected in one-month-old SD rats, from which the macrophages activated by the homogenate were collected from the abdominal cavity and cultured in vitro. The cells were divided into 4 groups according to different concentrations of FK506 for treatment, namely 0 (group A, control group), 0.25 ng/ml (group B), 0.5 ng/ml (group C), and 1.0 ng/ml (group D). The cells of the 4 groups were inoculated into 96-well plate respectively for detecting the viability of the macrophages by MTT assay and for morphological evaluation of the cell apoptosis by transmission electron microscopy and fluorescence microscopy.
  
  
  RESULTS
  The cells in groups B and C exhibited reduced viability and signs of apoptosis, and necrosis was observed in group D. Transmission electron microscopy and fluorescence microscopy identified early apoptotic changes and the presence of apoptotic body in the macrophages. The apoptotic rates of groups B and C were much higher than that in group A found by flow cytometry.
  
  
  CONCLUSIONS
  FK506 can promote the apoptosis of macrophage activated by allogenic nerve homogenate and reduce macrophage-mediated immunological rejection of peripheral nerve allograft.
authors.affiliation: Institute of Clinical Anatomy, First Military Medical University, Southern Medical University, Guangzhou 510515, China. yangjun1267@sina.com
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Qiu
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Yun-tao
authors.initials: YT
authors.lastname: Lu
authors.forename: Ying-jie
authors.initials: YJ
authors.lastname: Piao
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Qin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D017737:Macrophages, Peritoneal / Q000166:cytology*; D008297:Male; D009417:Nerve Tissue / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016559:Tacrolimus / Q000494:pharmacology*; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts; D016559:Tacrolimus