An alternative method for Plasmodium culture synchronization.
Since the synchronization of Plasmodium falciparum has become an essential tool in research, we have investigated the use of a commercial gelatine solution, Plasmion, to replace Plasmagel, which is now difficult to obtain. This method also avoids the use of techniques based on Percoll-glucose gradients. The Plasmion-based technique proved to be a good method and could become an alternative to Plasmagel.
Lelièvre
J
J
Laboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077 Toulouse, France.
Berry
A
A
Benoit-Vical
F
F
D000818:Animals; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology*; D005780:Gelatin / Q000031:analogs & derivatives*; D006801:Humans; D010952:Plasma Substitutes; D010963:Plasmodium falciparum / Q000502:physiology*
D010952:Plasma Substitutes; C025915:plasmagel; C013648:plasmion; D005780:Gelatin