title: Tbx3 expression is related to apoptosis and cell proliferation in rat bladder both hyperplastic epithelial cells and carcinoma cells.
abstract: In the present study, Tbx3, a member of the T-box family of transcription factors, was identified as an up-regulated gene by mRNA differential display in the regression (apoptosis) stage after uracil-induction of papillomatosis in the rat urinary bladder. Immunohistochemical analysis revealed that apoptosis cells are negative and apoptosis resistant cells are positive for Tbx3 expression. That suggests that Tbx3 is an apoptosis resistant gene rather than an apoptosis induced gene. We have found the rat bladder carcinoma cell line, BC31, to feature Tbx3 expression detectable by RT-PCR. To investigate its role in such cancer cells, they were transfected with an anti-sense Tbx3 expression vector. The obtained stable transfectant clones showed reduced expression of Tbx3 and much slower cell growth, as compared with mock transfectants, and many apoptotic cells were observed under normal culture conditions. These results indicate that Tbx3 is a negative regulator of apoptosis in bladder normal epithelial cells and suppression of Tbx3 expression causes inhibition of cell proliferation and induction of apoptosis in BC31, a rat bladder carcinoma cells.
authors.affiliation: Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Ito
authors.forename: Makoto
authors.initials: M
authors.lastname: Asamoto
authors.forename: Naomi
authors.initials: N
authors.lastname: Hokaiwado
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shirai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D049109:Cell Proliferation; D015870:Gene Expression; D015723:Gene Library; D006965:Hyperplasia / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D010212:Papilloma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D014162:Transfection; D015854:Up-Regulation; D014498:Uracil / Q000494:pharmacology; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism*; D019459:Urothelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D014498:Uracil