title: Perspectives in spicing up proteomics with splicing.
abstract: In the post-genomics era there has been an acceleration of understanding of cellular and organismal biology and this acceleration has moved the goalposts for proteomics. Higher eukaryotes use alternative promoters, alternative splicing, RNA editing and post-translational modification to produce multiple isoforms of proteins from single genes. Switching amongst these isoforms is a major mechanism for control of cellular function. At present fundamental limitations in sensitivity, in absolute quantitation of proteins and in the characterization of protein structure at functionally important levels strongly limit the applicability of proteomics to higher eukaryotes. Recent developments suggest that quantitative, top-down proteomics analyses of complete proteins at sub-attomole levels are necessary for physiologically relevant studies of higher eukaryotes. New proteomics technologies which will ensure the future of proteomics as an important technology in medicine and cellular biology of higher eukaryotes are becoming available.
authors.affiliation: Center for Molecular Medicine, Department of Medicine, University College London, London, UK. j.godovac-zimmermann@ucl.ac.uk
authors.forename: Jasminka
authors.initials: J
authors.lastname: Godovac-Zimmermann
authors.forename: Oliver
authors.initials: O
authors.lastname: Kleiner
authors.forename: Larry R
authors.initials: LR
authors.lastname: Brown
authors.forename: Andrzej K
authors.initials: AK
authors.lastname: Drukier
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D006801:Humans; D013058:Mass Spectrometry; D020033:Protein Isoforms / Q000378:metabolism; D019154:Protein Splicing; D021381:Protein Transport / Q000502:physiology*; D020543:Proteome / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D020033:Protein Isoforms; D020543:Proteome