title: Live birth following intracytoplasmic sperm injection after a 15-hour weather-related delay in cryopreservation of testicular sperm.
abstract: OBJECTIVE
  To report a live birth after transfer of cryopreserved blastocysts derived from testicular sperm that were cryopreserved 15 hours after biopsy due to inclement weather.
  
  
  METHODS
  Case report.
  
  
  METHODS
  University-affiliated private clinic.
  
  
  METHODS
  Couple undergoing IVF with intracytoplasmic sperm injection (ICSI) using testicular extracted sperm for male factor infertility due to unexplained obstructive azoospermia.
  
  
  METHODS
  An IVF case involving testicular biopsy, cryopreservation, and thawing of testicular tissue, ICSI, embryo freezing, and transfer of cyropreserved blastocysts.
  
  
  METHODS
  The ability to use cryopreserved testicular tissue in which optimal time from biopsy to freezing had been delayed because the weather was inclement.
  
  
  RESULTS
  In spite of a 15-hour delay in processing and cryopreservation of the testicular biopsy, subsequent thawing yielded viable sperm that resulted in viable embryos after ICSI. Transfer of fresh embryos was unsuccessful. However, subsequent use of supernumerary viable blastocysts that were frozen resulted in the live birth of a baby.
  
  
  CONCLUSIONS
  A 15-hour delay in processing and cryopreservation of a testicular biopsy did not affect the viability of the extracted sperm after thawing. Although it is desirable to freeze the testicular specimen as soon as possible, the delay in processing in this case due to inclement weather did not affect the potential for fertilization and implantation of a viable cryopreserved blastocyst.
authors.affiliation: Infertility and IVF Medical Associates of WNY, 4510 Main Street, Snyder, NY 14226, USA. hsbhakoo@yahoo.com
authors.forename: Hemlata S
authors.initials: HS
authors.lastname: Bhakoo
authors.forename: Peter
authors.initials: P
authors.lastname: Vaccaro
authors.forename: Kent
authors.initials: K
authors.lastname: Crickard
authors.forename: Michael W
authors.initials: MW
authors.lastname: Sullivan
mesh_terms: D000328:Adult; D001706:Biopsy; D001757:Blastomeres; D015925:Cryopreservation; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology*; D013737:Testis / Q000166:cytology; D013997:Time Factors; D014887:Weather