title: Cell wall localization of Red clover necrotic mosaic virus movement protein is required for cell-to-cell movement.
abstract: The Red clover necrotic mosaic virus movement protein (MP) is essential for cell-to-cell movement. Eight previously characterized alanine-scanning mutants of the MP were fused to the green fluorescent protein (GFP) and expressed from viral infectious transcripts. Inoculated plants were assayed for movement and intracellular accumulation of MP by confocal laser-scanning microscopy. A strict correlation was observed between the targeting to the cell wall (presumably the plasmodesmata) and cell-to-cell movement. Complementation of dysfunctional MP mutants with either wild-type MP or other null mutants in some cases rescued intracellular targeting and movement. The data suggest the presence of distinct domains in the MP for virus movement (near residues 27-31), complementarity (near residues 122 and 128), and intracellular localization (near residue 161). These data support a model of MP interacting cooperatively with itself to bind viral RNA, localize to and modify plasmodesmata and effect virus movement.
authors.affiliation: Department of Plant Pathology, Box 7616 North Carolina State University, Raleigh NC, 27695-7616 USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Tremblay
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Vaewhongs
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Turner
authors.forename: Tim L
authors.initials: TL
authors.lastname: Sit
authors.forename: Steven A
authors.initials: SA
authors.lastname: Lommel
mesh_terms: D000595:Amino Acid Sequence; D002473:Cell Wall / Q000502:physiology* / Q000821:virology; D009029:Mosaic Viruses / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D053830:Plant Viral Movement Proteins; D030821:Plants, Genetically Modified; D021381:Protein Transport; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014026:Nicotiana / Q000648:ultrastructure / Q000821:virology; D014764:Viral Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins