Experimental autoimmune neuritis induces differential microglia activation in the rat spinal cord.
The reactive spatial and temporal activation pattern of parenchymal spinal cord microglia was analyzed in rat experimental autoimmune neuritis (EAN). We observed a differential activation of spinal cord microglial cells. A significant increase in ED1(+) microglia predominantly located in the dorsal horn grey matter of lumbar and thoracic spinal cord levels was observed on Day 12. As revealed by morphological criteria and by staining with further activation markers [allograft inflammatory factor 1 (AIF-1), EMAPII, OX6, P2X(4)R], reactive microglia did not reach a macrophage-like state of full activation. On Day 12, a significant proliferative response could be observed, affecting all spinal cord areas and including ED1(+) microglial cells and a wide range of putative progenitor cells. Thus, in rat EAN, a reactive localized and distinct microglial activation correlating with a generalized proliferative response could be observed.
Beiter
Thomas
T
Institute of Brain Research, University of Tuebingen, Calwer Str.3, D-72076 Tuebingen, Germany. hirnforschung@uni-tuebingen.de
Artelt
Matthias R
MR
Trautmann
Katrin
K
Schluesener
Hermann J
HJ
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000276:immunology; D049109:Cell Proliferation; D053331:Ectodysplasins; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism; D008840:Microfilament Proteins; D017628:Microglia / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D011917:Rats, Inbred Lew; D012720:Severity of Illness Index; D013116:Spinal Cord / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*
C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D053331:Ectodysplasins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D001973:Bromodeoxyuridine