title: Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound.
abstract: Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-richconditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans.
authors.affiliation: Department of Molecular Biology, Medical University Innsbruck, A-6020 Innsbruck, Austria.
authors.forename: Markus
authors.initials: M
authors.lastname: Schrettl
authors.forename: Günther
authors.initials: G
authors.lastname: Winkelmann
authors.forename: Hubertus
authors.initials: H
authors.lastname: Haas
mesh_terms: D000595:Amino Acid Sequence; D001681:Biological Assay; D001692:Biological Transport; D002851:Chromatography, High Pressure Liquid; D005291:Ferrichrome / Q000737:chemistry*; D005656:Fungal Proteins; D006877:Hydroxamic Acids / Q000737:chemistry; D007501:Iron / Q000378:metabolism*; D013058:Mass Spectrometry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012568:Schizosaccharomyces / Q000378:metabolism* / Q000502:physiology
chemical_list: D005656:Fungal Proteins; D006877:Hydroxamic Acids; D005291:Ferrichrome; D007501:Iron