Antiapoptotic and antioxidant effects of rosmarinic acid in astrocytes.
The protective effects of rosmarinic acid (RA), a polyphenolic compound, on apoptosis induced by hydrogen peroxide in astrocytes were studied. Pretreating cells with RA significantly increased cell viability and decreased apoptosis rate induced by H2O2. The antiapoptotic effect of RA was further confirmed by increase of mitochondrial membrane potential and inhibition of caspase-3 activity. RA also attenuated cellular oxidative stress by decreasing the amount of reactive oxygen species and malondialdehyde. Results clearly show that RA was able to attenuate H2O2-induced cell injury by its antiapoptotic and antioxidant activity.
Gao
L P
LP
School of Life Sciences, Lanzhou University, Lanzhou Medical College, P R China.
Wei
H L
HL
Zhao
H S
HS
Xiao
S Y
SY
Zheng
R L
RL
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology*; D053630:Depsides; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology; D051381:Rats; D000097123:Rosmarinic Acid
D000975:Antioxidants; D053148:Caspase 3; D020169:Caspases; D002934:Cinnamates; D053630:Depsides; D006861:Hydrogen Peroxide; D016877:Oxidants; D000097123:Rosmarinic Acid; C505410:Casp3 protein, rat