title: [Detection of hepatotoxic pyrrolizidine alkaloids in Ligularia Cass. with LC/MSn].
abstract: OBJECTIVE
  To detect the hepatotoxic pyrrolizidine alkaloids (HPA) in the genus Ligularia Cass..
  
  
  METHODS
  The alkaloid extracts of Ligularia plant materials were detected and analyzed by the method of combination of TLC, and LC/MSn.
  
  
  RESULTS
  Among 22 species of Ligularia Cass., HPA were detected in 18 species with LC/MSn, and no HPA was detected in the remaining 4 species.
  
  
  CONCLUSIONS
  HPA was first detected with LC/MSn in L. tongelensis and other 15 species of Ligularia Cass.; HPA from these plants should be isolated, separated and identified and it is necessary to study the activities and toxicities of the HPA. The types and kinds of HPA from different species and sources are different, they should be detected separately.
authors.affiliation: College of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210038, China.
authors.forename: She-ban
authors.initials: SB
authors.lastname: Pu
authors.forename: De-ran
authors.initials: DR
authors.lastname: Xu
authors.forename: Mian
authors.initials: M
authors.lastname: Zhang
authors.forename: Hong-hua
authors.initials: HH
authors.lastname: Zhou
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Guo-dian
authors.initials: GD
authors.lastname: Yu
mesh_terms: D019659:Asteraceae / Q000737:chemistry* / Q000145:classification; D002855:Chromatography, Thin Layer; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000737:chemistry; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D011763:Pyrrolizidine Alkaloids; C412858:otonecine; C014795:retronecine