title: Cloning of duck PRL cDNA and genomic DNA.
abstract: Complementary DNA (cDNA) and genomic DNA, including flanking regions of the prolactin (PRL) gene of domesticated duck, were cloned and sequenced. Duck PRL was found to have 92.0, 91.7, and 91.4% sequence identity at the cDNA level to PRL of chicken, turkey, and quail, respectively. The predicted amino acid sequence had an overall similarity with a comparable region of chicken (93.4%), turkey (91.3%), and quail (91.3%) PRL. Mature duck PRL contains the consensus sequence for N-linked glycoslylation at position 6 which is not present in either chickens or turkeys. Thus, duck PRL is likely to be post-translationally modified differently from other avian species. Based on the cDNA sequence, the genomic structure of the gene was characterized. The duck PRL gene consists of 5 exons and 4 introns. Moreover, sequence analysis of the proximal region of duck PRL promoter revealed a high degree of similarity to that of chicken and turkey PRL promoter. These results suggest that the mechanisms, which regulate expression of the PRL gene, may be widely conserved in avian species.
authors.affiliation: Laboratory of Animal Genetics and Breeding, Azabu University, Fuchinobe, Sagamihara 229-8501, Japan. kansaku@azabu-u.ac.jp
authors.forename: Norio
authors.initials: N
authors.lastname: Kansaku
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Hisato
authors.initials: H
authors.lastname: Okabayashi
authors.forename: Daniel
authors.initials: D
authors.lastname: Guémené
authors.forename: Urs
authors.initials: U
authors.lastname: Kuhnlein
authors.forename: David
authors.initials: D
authors.lastname: Zadworny
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Shimada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004372:Ducks / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D011388:Prolactin / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D011388:Prolactin