[Purification and relative molecular mass determination of tea glycoprotein by high performance liquid chromatography-electrospray ionisation mass spectrometry].
Tea glycoprotein (TGC) was extracted from coarse tea leaves produced in Jiangxi Province. The separation and purification were carried out by using gel chromatography and high performance liquid chromatography. Furthermore, relative molecular mass (M(r)) of TGC was determined by gel chromatography and high performance liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MS), respectively. It is 51 500.
Zhou
Peng
P
Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China.
Xie
Mingyong
M
Wang
Yuanxing
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013662:Tea / Q000737:chemistry*
D006023:Glycoproteins; D013662:Tea