title: Molecular cloning and functional characterization of a high-affinity zinc importer (DrZIP1) from zebrafish (Danio rerio).
abstract: Zinc is a vital micronutrient to all organisms and a potential toxicant to aquatic animals. It is therefore of importance to understand the mechanism of zinc regulation. In the present study, we molecularly cloned and functionally characterized a zinc transporter of the SLC39A family [commonly referred to as the ZIP (Zrt- and Irt-related protein) family] from the gill of zebrafish (Danio rerio) (DrZIP1). DrZIP1 protein was found to localize at the plasma membrane and to function as a zinc uptake transporter when being expressed in either chinook salmon (Oncorhynchus tshawytscha) embryonic 214 cells or Xenopus laevis oocytes. In comparison with pufferfish transporter proteins (FrZIP2 and FrECaC) that are known to facilitate cellular zinc uptake, DrZIP1 appears to have high affinity to bind and transport zinc, suggesting that it maybe a high-affinity zinc uptake transporter (Km < 0.5 microM) in fish. Orthologues of DrZIP1 were also identified in both freshwater and seawater pufferfish (Tetraodon nigroviridis and Takifugu rubripes), indicating that these proteins may be functionally conserved among different fish species. DrZIP1 mRNA is expressed in all the tissues examined in the present study and thus DrZIP1 may be a constitutive zinc uptake transporter in many cell types of zebrafish.
authors.affiliation: School of Health and Life Sciences, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NN, UK.
authors.forename: Andong
authors.initials: A
authors.lastname: Qiu
authors.forename: Majid
authors.initials: M
authors.lastname: Shayeghi
authors.forename: Christer
authors.initials: C
authors.lastname: Hogstrand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010802:Phylogeny; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012474:Salmon; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D023721:Tetraodontiformes; D014982:Xenopus laevis; D015027:Zebrafish / Q000235:genetics*; D015032:Zinc / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; D012333:RNA, Messenger; C106542:SLC39A3 protein, human; D015032:Zinc