[On the new mechanism of adaptive response].
The role of changes in cell composition of population of human blood lymphocytes in the forming of an adaptive response (AR) has been studied. By micronuclei assay and cytokinetic block with cytohalasin B the frequency of mono-, bi- and multinuclear cells with micronuclei (MN) and without MN were determined in the initial population. The same parameters have been studied after exposure of the population to the adaptive (0.05 Gy), challenge (1.0 Gy) doses and to doses 0.05 + 1.0 Gy 5 hours after. 13 from 23 investigated individuals manifested the AR: the decreasing of the ratio of damaged binuclear cells to the all binuclear cells after the adaptive and challenge exposure. It was shown that the ways of an AR forming are different: in 7 of 13 individuals with AR the number of binuclear cells with MN did not decrease but the amount of binuclear undamaged cells increased. The ratio of these parameters enhances but not for the account of cells with MN decreasing. There is the linear correlation between the frequency of cells with MN and the frequency of binuclear cells in population (spontaneous, after irradiation with doses of 0.05, 1.0 and 0.05 + 1.0 Gy) with the coefficient of correlation about -1. These results show the presence of new mechanism of AR forming, which is not connect with the induction of damage repair and rather with the stimulation of cell division. In the another group of individuals the decrease in damaged cells number after irradiation with doses of 0.05 + 1.0 Gy have been observed. Probably the stimulation of repair system occurred to the moment of 1.0 Gy irradiation. Thus, the mechanism of an AR forming depends on the individual properties of organism. The work was suppoted by RFBR grant 03-04-48325a.
Serebrianyĭ
A M
AM
Aleshchenko
A V
AV
Gotlib
V Ia
VIa
Kudriashova
O V
OV
Semenova
L P
LP
Pelevina
I I
II
D000222:Adaptation, Physiological; D000293:Adolescent; D000328:Adult; D002467:Cell Nucleus / Q000528:radiation effects; D002648:Child; D003571:Cytochalasin B / Q000494:pharmacology; D048749:Cytokinesis / Q000187:drug effects; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000097:blood / Q000166:cytology / Q000528:radiation effects; D008297:Male; D015162:Micronucleus Tests; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000502:physiology; D011839:Radiation, Ionizing
D003571:Cytochalasin B