[Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing].
OBJECTIVE
To explore an effective method for detecting human papillomavirus (HPV) DNA in cervical cancer tissue.


METHODS
HPV L1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR with consensus primers, and typing of the HPV strains was performed on the basis of sequence analysis of the PCR product.


RESULTS
The positivity rates of HPV DNA was 78% in the 50 cases of cervical cancer, and mixed infection with HPV16 and HPV18 strains was the most common, which accounted for 48% on the total infections. Infection with HPV58 was detected in one case. The sequencing results showed no difference in L1 sequence between the detected samples and the standard German HPV58 strain.


CONCLUSIONS
PCR and direct sequencing approach is effective for detecting and typing of HPV DNA in cervical cancer tissue, through which rare HPV strain or mutants of known HPV strains may not escape detection.
Liu
Xin
X
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. liuxin23@fimmu.com
Liu
Guo-Bing
GB
Pang
Zhan-Jun
ZJ
Xing
Fu-Qi
FQ
Guo
Sui-Qun
SQ
Ma
Wen-Li
WL
Zheng
Wen-Ling
WL
D000230:Adenocarcinoma / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D004279:DNA, Viral