Drosophila couch potato mutants exhibit complex neurological abnormalities including epilepsy phenotypes.
RNA-binding proteins play critical roles in regulation of gene expression, and impairment can have severe phenotypic consequences on nervous system function. We report here the discovery of several complex neurological phenotypes associated with mutations of couch potato (cpo), which encodes a Drosophila RNA-binding protein. We show that mutation of cpo leads to bang-sensitive paralysis, seizure susceptibility, and synaptic transmission defects. A new cpo allele called cpo(EG1) was identified on the basis of a bang-sensitive paralytic mutant phenotype in a sensitized genetic background (sda/+). In heteroallelic combinations with other cpo alleles, cpo(EG1) shows an incompletely penetrant bang-sensitive phenotype with approximately 30% of flies becoming paralyzed. In response to electroconvulsive shock, heteroallelic combinations with cpo(EG1) exhibit seizure thresholds less than half that of wild-type flies. Finally, cpo flies display several neurocircuit abnormalities in the giant fiber (GF) system. The TTM muscles of cpo mutants exhibit long latency responses coupled with decreased following frequency. DLM muscles in cpo mutants show drastic reductions in following frequency despite exhibiting normal latency relationships. The labile sites appear to be the electrochemical GF-TTMn synapse and the chemical PSI-DLMn synapses. These complex neurological phenotypes of cpo mutants support an important role for cpo in regulating proper nervous system function, including seizure susceptibility.
Glasscock
Edward
E
Department of Molecular and Cell Biology, Division of Neurobiology, University of California, Berkeley, 94720, USA.
Tanouye
Mark A
MA
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology*; D004563:Electrochemistry; D004594:Electrophysiology; D004597:Electroshock; D004625:Embryo, Nonmammalian; D004742:Enhancer Elements, Genetic; D004827:Epilepsy / Q000235:genetics*; D005260:Female; D005816:Genetic Complementation Test; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D009154:Mutation; D009420:Nervous System / Q000196:embryology*; D009424:Nervous System Physiological Phenomena; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D012640:Seizures / Q000235:genetics; D013569:Synapses / Q000378:metabolism
D029721:Drosophila Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; C078019:cpo protein, Drosophila; D012313:RNA