Proteome of Methanosarcina acetivorans Part II: comparison of protein levels in acetate- and methanol-grown cells.
Methanosarcina acetivorans is an archaeon isolated from marine sediments which utilizes a diversity of substrates for growth and methanogenesis. Part I of a two-part investigation has profiled proteins of this microorganism cultured with both methanol and acetate as growth substrates, utilizing two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometry. In this report, Part II, the analyses were extended to identify 34 proteins found to be present in different amounts between methanol- and acetate-grown M. acetivorans. Among these proteins are enzymes which function in pathways for methanogenesis from either acetate or methanol. Several of the 34 proteins were determined to have redundant functions based on annotations of the genomic sequence. Enzymes which function in ATP synthesis and steps common to both methanogenic pathways were elevated in acetate- versus methanol-grown cells, whereas enzymes that have a more general function in protein synthesis were in greater amounts in methanol- compared to acetate-grown cells. Several group I chaperonins were present in greater amounts in methanol- versus acetate-grown cells, whereas lower amounts of several stress related proteins were found in methanol- versus acetate-grown cells. The potential physiological basis for these novel patterns of protein synthesis are discussed.
Li
Qingbo
Q
Center for Microbial Structural Biology, Department of Biochemistry and Molecular Biology, 205 South Frear Laboratory, Penn State University, University Park, Pennsylvania 16802, USA.
Li
Lingyun
L
Rejtar
Tomas
T
Karger
Barry L
BL
Ferry
James G
JG
D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis*; D018833:Chaperonins / Q000302:isolation & purification; D006360:Heat-Shock Proteins / Q000302:isolation & purification; D013058:Mass Spectrometry; D000432:Methanol / Q000378:metabolism; D017020:Methanosarcina / Q000737:chemistry* / Q000254:growth & development; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000085:Acetates; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D020543:Proteome; D018833:Chaperonins; D000432:Methanol