[The effect of lishi No.5 formula on the growth of human neuroblastoma cell line SY5Y].
OBJECTIVE
To investigate the effect of Lishi No.5 formula on the growth of human neuroblastoma cell line SY5Y and find out the most effective drug dose.


METHODS
SY5Y cells were administrated by Lishi No.5 formula in different concentration. MTT metabolic rate was measured as the cell survival rate, then the dose-effect curve was made. LDH leakage rate, axonal length and area of cell body were used as the indicators.


RESULTS
In 0.125-0.75 g x L(-1) Lishi No. 5 formula could increase the cell survival rate and MTT metabolic rate, decreased LDH leakage rate, and enhance axonal length and area of cell body, compared with control group.


CONCLUSIONS
Lishi No.5 formula has the neurotrophic effect and can induce the survival of neuron.
Zhang
Jing-yan
JY
Beijing Research Laboratory for Brain Aging, Beijing Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
Wang
Rong
R
Li
Zi-zhong
ZZ
Cheng
Hao-ran
HR
Li
Sheng-hai
SH
Liu
Meng-xia
MX
Sheng
Shu-li
SL
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase