In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level.
The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A' reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level.
Croitoru
Victor V
VV
Department of Genetics, Microbiology and Toxicology, Stockholm University, S-10691 Stockholm, Sweden.
Bucheli-Witschel
Margarete
M
Isaksson
Leif A
LA
D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D039861:Prokaryotic Initiation Factor-1 / Q000235:genetics* / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013696:Temperature
D003062:Codon; D039861:Prokaryotic Initiation Factor-1; D012333:RNA, Messenger