title: Effect of dietary anti-urease immunoglobulin Y on Helicobacter pylori infection in Mongolian gerbils.
abstract: OBJECTIVE
  Helicobacter pylori is known to be a major pathogenic factor in the development of gastritis, peptic ulcer disease and gastric cancer. Recently, chicken egg yolk immunoglobulin Y (IgY) has been recognized as an inexpensive antibody source for passive immunization against gastrointestinal infections. The present study was designed to investigate the effect of anti-urease IgY on H. pylori infection in Mongolian gerbils.
  
  
  METHODS
  H. pylori-infected Mongolian gerbils were administered a diet containing anti-urease IgY, with or without famotidine (F). After 10 weeks, bacterial culture and measurement of the gastric mucosal myeloperoxidase (MPO) activity were performed. In a second experiment, another group of gerbils was started on a diet containing F + IgY a week prior to H. pylori inoculation. After 9 weeks, these animals were examined.
  
  
  RESULTS
  In the H. pylori-infected gerbils, there were no significant differences in the level of H. pylori colonization among the different dietary and control groups. However, the MPO activity was significantly decreased in the H. pylori group administered the F + IgY diet compared with that in the H. pylori group administered the IgY, F, or control diet. Furthermore, in the gerbils administered the F + IgY diet prior to the bacterial inoculation, inhibition of H. pylori colonization and suppression of the elevated gastric mucosal MPO activity were observed.
  
  
  CONCLUSIONS
  Oral administration of urease-specific IgY not only inhibited H. pylori disease activity in H. pylori-infected gerbils, but also prevented H. pylori colonization in those not yet infected. These encouraging results may pave the way for a novel therapeutic and prophylactic approach in the management of H. pylori-associated gastroduodenal disease.
authors.affiliation: Center for Integrated Medical Research, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nomura
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Suzuki
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Masaoka
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kurabayashi
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Ishii
authors.forename: Masaki
authors.initials: M
authors.lastname: Kitajima
authors.forename: Kikuo
authors.initials: K
authors.lastname: Nomoto
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Hibi
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage; D004032:Diet; D004195:Disease Models, Animal; D015738:Famotidine / Q000008:administration & dosage; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005849:Gerbillinae / Q000382:microbiology*; D016481:Helicobacter Infections / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007136:Immunoglobulins / Q000008:administration & dosage*; D008297:Male; D009195:Peroxidase / Q000032:analysis; D014510:Urease / Q000276:immunology*
chemical_list: D000897:Anti-Ulcer Agents; C016512:IgY; D007136:Immunoglobulins; D015738:Famotidine; D009195:Peroxidase; D014510:Urease