Inactivation of soybean trypsin inhibitors and lipoxygenase by high-pressure processing.
Trypsin inhibitors (TIA), one of the antinutritional factors of soy milk, are usually inactivated by heat treatment. In the current study, high-pressure processing (HPP) was evaluated as an alternative for the inactivation of TIA in soy milk. Moreover, the effect of HPP on lipoxygenase (LOX) in whole soybeans and soy milk was studied. For complete LOX inactivation either very high pressures (800 MPa) or a combined temperature/pressure treatment (60 degrees C/600 MPa) was needed. Pressure inactivation of TIA was possible only in combination with elevated temperatures. For TIA inactivation, three process parameters, temperature, time, and pressure, were optimized using experimental design and response surface methodology. A 90% TIA inactivation with treatment times of <2 min can be reached at temperatures between 77 and 90 degrees C and pressures between 750 and 525 MPa.
van der Ven
Cornelly
C
Wageningen UR, Agrotechnology and Food Innovations, P.O. Box 17, 6700 AA Wageningen, The Netherlands. cornelly.vanderven@wur.nl
Matser
Ariette M
AM
van den Berg
Robert W
RW
D006358:Hot Temperature; D008084:Lipoxygenase / Q000378:metabolism*; D011312:Pressure; D041942:Soy Milk / Q000737:chemistry; D013025:Glycine max / Q000201:enzymology; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000378:metabolism*
D014360:Trypsin Inhibitor, Kunitz Soybean; D008084:Lipoxygenase