The effects of the Penicillium mycotoxins citrinin, cyclopiazonic acid, ochratoxin A, patulin, penicillic acid, and roquefortine C on in vitro proliferation of porcine lymphocytes.
The in vitro effect of each of the Penicillium mycotoxins citrinin (CIT), cyclopiazonic acid (CPA), ochratoxin A (OTA), patulin (PAT), penicillic acid (PIA) and roquefortine C (RQC) on mitogen induced lymphocyte proliferation was determined using purified lymphocytes from 6 piglets. Dose response curves for each mycotoxin were generated and the concentrations producing 50% inhibition of cell proliferation (IC(50)) were estimated. OTA and PAT were the most potent toxins with IC(50) of 1.3 and 1.2 micromol/l, respectively (0.52 and 0.18 mg/l, respectively). Based on molar concentrations, OTA was 15, 30, 40, and 65 times more potent as an inhibitor than PIA, CIT, CPA and RQC, respectively.
Keblys
Modestas
M
National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway.
Bernhoft
Aksel
A
Höfer
Constance C
CC
Morrison
Ellen
E
Larsen
Hans Jørgen S
HJ
Flåøyen
Arne
A
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002953:Citrinin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D009183:Mycotoxins / Q000494:pharmacology*; D009793:Ochratoxins / Q000494:pharmacology; D010365:Patulin / Q000494:pharmacology; D010398:Penicillic Acid / Q000494:pharmacology; D010407:Penicillium / Q000737:chemistry*; D010879:Piperazines / Q000494:pharmacology; D013552:Swine
D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D009183:Mycotoxins; D009793:Ochratoxins; D010879:Piperazines; C025589:ochratoxin A; D002953:Citrinin; C012536:roquefortine; D010365:Patulin; D010398:Penicillic Acid