title: No effect of platelet-rich plasma with frozen or processed bone allograft around noncemented implants.
abstract: We compared processed morselized bone allograft with fresh-frozen bone graft around noncemented titanium implants. Also, the influence of platelet-rich plasma (PRP) in combination with bone allograft was evaluated. Analysis was based on implant fixation and histomorphometry. PRP was prepared by isolating the buffy coat from autologous blood samples. Bone allograft was used fresh-frozen or processed by defatting, freeze drying, and irradiation. Cylindrical hydroxyapatite-coated titanium implants were inserted bilaterally in the femoral condyles of eight dogs. Each implant was surrounded by a 2.5-mm concentric gap, which was filled randomly according to the four treatment groups--group 1: fresh-frozen bone allograft; group 2: processed bone allograft; group 3: fresh-frozen bone allograft + PRP; group 4: processed bone allograft + PRP. Histological and mechanical evaluation demonstrated no influence of bone allograft processing. Even though the level of platelet in PRP was 7.7 times that found in whole blood, we found no improvement of bone formation or implant fixation by adding PRP.
authors.affiliation: Orthopaedic Research Group, Aarhus University Hospital, Aarhus, Denmark. tbj@webspeed.dk
authors.forename: T B
authors.initials: TB
authors.lastname: Jensen
authors.forename: O
authors.initials: O
authors.lastname: Rahbek
authors.forename: S
authors.initials: S
authors.lastname: Overgaard
authors.forename: K
authors.initials: K
authors.lastname: Søballe
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001792:Blood Platelets / Q000502:physiology*; D016025:Bone Transplantation / Q000379:methods / Q000502:physiology*; D004285:Dogs; D017886:Durapatite / Q000627:therapeutic use; D017102:Fracture Healing / Q000187:drug effects*; D007595:Joint Prosthesis; D016348:Osseointegration / Q000187:drug effects / Q000502:physiology; D010949:Plasma / Q000502:physiology*; D013048:Specimen Handling / Q000379:methods; D014025:Titanium / Q000627:therapeutic use
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite; D014025:Titanium