title: HPLC quantification of uncarine D and the anti-plasmodial activity of alkaloids from leaves of Mitragyna inermis (Willd.) O. Kuntze.
abstract: An efficient system for the analysis of the total alkaloids extracted from leaves of Mitragyna inermis (Willd.) O. Kuntze (Rubiaceae) by HPLC using a reversed-phase column is described. The chromatographic conditions allowed the separation of indole and oxindole alkaloids in leaf extracts, and the quantification of uncarine D in samples collected in Burkina Faso and Mali. The HPLC method described was validated for its specificity, linearity and precision using an internal standard (naphthalene). The concentrations of uncarine D in various extracts were compared with their in vitro anti-plasmodial activity. The anti-proliferative activity on chloroquine-resistant strain (W2) of Plasmodium falciparum was not correlated with the concentration of uncarine D in leaves.
authors.affiliation: Laboratoire de Pharmacognosie, Faculté de Pharmacie, 13385 Marseille Cedex 5, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Fiot
authors.forename: Béatrice
authors.initials: B
authors.lastname: Baghdikian
authors.forename: Laurent
authors.initials: L
authors.lastname: Boyer
authors.forename: Valérie
authors.initials: V
authors.lastname: Mahiou
authors.forename: Nadine
authors.initials: N
authors.lastname: Azas
authors.forename: Monique
authors.initials: M
authors.lastname: Gasquet
authors.forename: Pierre
authors.initials: P
authors.lastname: Timon-David
authors.forename: Guy
authors.initials: G
authors.lastname: Balansard
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ollivier
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032065:Mitragyna / Q000737:chemistry*; D015394:Molecular Structure; D000078183:Oxindoles; D018515:Plant Leaves / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects; D015203:Reproducibility of Results
chemical_list: D000470:Alkaloids; D000962:Antimalarials; D000078183:Oxindoles; C104393:speciophylline