title: Testicular toxicity induced by dietary cadmium is associated with decreased testicular zinc and increased hepatic and renal metallothionein and zinc in the bank vole (Clethrionomys glareolus).
abstract: Mechanism of testicular toxicity induced by dietary cadmium (Cd) has been less investigated than that following acute Cd injection. In the present study we characterized testicular injury in a small rodent, the bank vole, exposed subchronically to dietary Cd in a quantity of 0.9 micromol/g, and determined the importance of some factors (Cd accumulation, metallothionein (MT), oxidative stress, and zinc (Zn)) in the injury. Dietary Cd induced moderate histopathological changes (hemorrhage in interstitium, necrosis and apoptosis in seminiferous tubule epithelium) in young (1 month old) bank voles fed, for 6 weeks, Fe-adequate (1.1-1.4 micromol/g) and Fe-enriched (4.5-4.8 micromol/g) diets. In contrast, adult (5 months old) bank voles appeared to be resistant to the toxic effects of dietary Cd, despite the fact that testicular Cd contents were higher and MT levels lower than those in the young animals. The Cd-induced histopathological changes and apoptosis were accompanied by increased testicular lipid peroxidation, decreased testicular Zn concentration and elevated levels of hepatic and renal MT and Zn. Supplemental dietary Zn (1.7-1.8 micromol/g) prevented the Cd-induced testicular Zn depletion and injury. The data indicate that dietary Cd produces testicular lesions indirectly, through decreasing testicular Zn, which seems to be due to the sequestration of this element by the Cd-induced hepatic and renal MT.
authors.affiliation: Institute of Biology, University of Białystok, Swierkowa 20B, 15-950 Białystok, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Bonda
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Włostowski
authors.forename: Alicja
authors.initials: A
authors.lastname: Krasowska
mesh_terms: D000818:Animals; D017209:Apoptosis; D003411:Arvicolinae; D001835:Body Weight; D002104:Cadmium / Q000009:adverse effects* / Q000378:metabolism; D004032:Diet; D020287:In Situ Nick-End Labeling; D007668:Kidney / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D018384:Oxidative Stress; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D015032:Zinc / Q000378:metabolism*
chemical_list: D002104:Cadmium; D008668:Metallothionein; D015032:Zinc