Overexpression of DARPP-32 in colorectal adenocarcinoma.
Dopamine and cAMP-regulated phosphoprotein (DARPP)-32 is a phosphoprotein that is centrally involved in dopamine signalling pathways. It may play an important role in gastric tumorigenesis. Our aim is to investigate the expression of DARPP-32 protein in colorectal adenocarcinoma. The expression of DARPP-32 was evaluated by immunohistochemical SABC staining and Western blotting. DARPP-32 was specifically localised in colorectal epithelium. The expression of DARPP-32 in colorectal adenocarcinoma tissues 33/42, 78.57% was higher than that in normal colon epithelial tissues (31/60, 51.67%, p <0.05). There was no significant relationship between the expression of DARPP-32 and the differentiation, metastasis and Dukes' stage of colorectal adenocarcinoma (p >0.05). Both DARPP-32 and its truncated isoform t-DARPP were overexpressed in colorectal adenocarcinoma (t=2.306, p=0.028), while t-DARPP was more frequently detected. These results showed that DARPP-32 may play an important role in the regulation of normal colorectal epithelial biology and carcinogenesis.
Wang
M S J
MS
Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Pan
Y
Y
Liu
N
N
Guo
C
C
Hong
L
L
Fan
D
D
D000230:Adenocarcinoma / Q000378:metabolism*; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000378:metabolism*; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D006801:Humans; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins