Flavonoids from the leaves of Diospyros kaki reduce hydrogen peroxide-induced injury of NG108-15 cells.
Flavonoids from the leaves of Diospyros kaki L (FLDK-P70) are main therapeutic components of NaoXingQing, which is a novel and patented Traditional Chinese Medicine drug used for the treatment of syndrome of apoplexy for years in China. The present study investigated the effects of FLDK-P70 on hydrogen peroxide (H2O2)-induced apoptosis-like damage of NG108-15 cells. Pretreatment of cells with FLDK-P70 alleviated H2O2-induced cell injury and apoptosis by upregulating Bcl-2 expression and improving redox imbalance as indicated by the alleviation of the decline in the intracellular endogenous antioxidants: glutathione and glutathione peroxidase as well as catalase, with the decrease of the leak of lactate dehydrogenase and the reduction of the accumulation of malondialdehyde. These results indicate that FLDK-P70 may be potentially used in the prevention and treatment of ischemia/reperfusion injury and other neurodegenerative disease. Upregulating bcl-2 and improving cellular redox state by FLDK-P70 may play critical roles in attenuating oxidative injury.
Bei
Weijian
W
State Key Laboratory of Biocontrol, Department of Biochemistry and Center for Biopharmaceutical Research, School of Life Sciences, Sun Yatsen (Zhongshan) University, 135 W. XinGang Road, Guangzhou, 510275, PR China.
Peng
Wenlie
W
Ma
Yan
Y
Xu
Anlong
A
D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D029788:Diospyros / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D009447:Neuroblastoma / Q000188:drug therapy / Q000473:pathology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D018515:Plant Leaves / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects; D015854:Up-Regulation
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D019253:Proto-Oncogene Proteins c-bcl-2; D006861:Hydrogen Peroxide