High-level acetaldehyde production in Lactococcus lactis by metabolic engineering.
Efficient conversion of glucose to acetaldehyde is achieved by nisin-controlled overexpression of Zymomonas mobilis pyruvate decarboxylase (pdc) and Lactococcus lactis NADH oxidase (nox) in L. lactis. In resting cells, almost 50% of the glucose consumed could be redirected towards acetaldehyde by combined overexpression of pdc and nox under anaerobic conditions.
Bongers
Roger S
RS
NIZO food research, Department of Flavour and Natural Ingredients, P.O. Box 20, 6710 BA Ede, The Netherlands. Roger.Bongers@nizo.nl
Hoefnagel
Marcel H N
MH
Kleerebezem
Michiel
M
D000079:Acetaldehyde / Q000378:metabolism*; D000693:Anaerobiosis; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011767:Pyruvate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology* / Q000235:genetics
D003470:Culture Media; D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D011767:Pyruvate Decarboxylase; D000079:Acetaldehyde