Differential expression of Ixodes ricinus tick genes induced by blood feeding or Borrelia burgdorferi infection.
Ixodes ricinus L. is the principal European vector of Borrelia burgdorferi sensu lato, the causative agent of Lyme borreliosis. Subtractive hybridization was used to isolate tick genes that were induced in whole ticks after blood meals on uninfected and B. burgdorferi-infected guinea pigs. Novel cDNA clones with similarity to cytochrome c oxidase, salivary secreted protein, actin, and a cysteine protease propeptide were induced after a blood meal. Novel cDNA clones with similarity to thioredoxin peroxidases, dolichyl-phosphate beta-glucosyltransferase, glutathione S-transferase, defensin, ML domain-containing protein, and von Willebrand factor were induced after B. burgdorferi infection. Virtual Northern analysis was used to verify that these genes were differentially expressed in ticks after a pathogen-infected blood meal and to detect their tissues of expression. The characterization of genes that are induced after an infected blood meal is essential for gaining an understanding of the molecular mechanisms that underlie vector-pathogen interactions.
Rudenko
Nataliia
N
Faculty of Biological Sciences, University of South Bohemia in Ceské Budejovice and Institute of Parasitology of the Academy of Sciences of the Czech Republic, Ceské Budejovice 37005, the Czech Republic. natasharudenko@hotmail.com
Golovchenko
Maryna
M
Edwards
Marten J
MJ
Grubhoffer
Libor
L
D000818:Animals; D001769:Blood; D025065:Borrelia burgdorferi; D018076:DNA, Complementary / Q000235:genetics; D004435:Eating; D005502:Food; D005786:Gene Expression Regulation / Q000502:physiology*; D015723:Gene Library; D017344:Genes, Insect / Q000235:genetics*; D006168:Guinea Pigs; D007303:Insect Vectors; D018884:Ixodes / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D008193:Lyme Disease / Q000097:blood* / Q000635:transmission; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species; D013987:Ticks / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D018076:DNA, Complementary; D017382:Reactive Oxygen Species