title: Morphological criteria to distinguish cell death induced by apoptotic and necrotic treatments.
abstract: We present a comparative study of apoptotic and necrotic morphology (light and scanning electron microscopy), induced by well known experimental conditions (photodynamic treatments, etoposide, hydrogen peroxide, freezing-thawing and serum deprivation) on cell cultures. Our results indicate that morphological criteria (apoptotic cell rounding and shrinkage, and appearance of membrane bubbles in early necrosis) allow to distinguish these cell death mechanisms, and also show that, independently of the damaging agents, the necrotic process occurs in a characteristic sequence (coalescence of membrane bubbles in a single big one that detaches from cells remaining on the substrate).
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Ciudad Universitaria de Cantoblanco, E-28049, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Rello
authors.forename: J C
authors.initials: JC
authors.lastname: Stockert
authors.forename: V
authors.initials: V
authors.lastname: Moreno
authors.forename: A
authors.initials: A
authors.lastname: Gámez
authors.forename: M
authors.initials: M
authors.lastname: Pacheco
authors.forename: A
authors.initials: A
authors.lastname: Juarranz
authors.forename: M
authors.initials: M
authors.lastname: Cañete
authors.forename: A
authors.initials: A
authors.lastname: Villanueva
mesh_terms: D017209:Apoptosis; D016923:Cell Death; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009336:Necrosis / Q000473:pathology*; D016877:Oxidants / Q000494:pharmacology; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000494:pharmacology; D011166:Porphyrins / Q000494:pharmacology
chemical_list: C467708:5-(4-N-(N-2',6'-dinitro-4'-trifluoromethylphenyl)aminophenyl)-10,15,20-tris(2,4,6-trimethoxyphenyl)porphyrin; D016877:Oxidants; D017319:Photosensitizing Agents; D011166:Porphyrins; D006861:Hydrogen Peroxide; D010696:Phloroglucinol