title: Selective preparation of N-acetyl-D-glucosamine and N,N'-diacetylchitobiose from chitin using a crude enzyme preparation from Aeromonas sp.
abstract: A bacterium, Aeromonas sp. GJ-18, having strong chitinolytic activity was isolated from coastal soil and used for crude enzyme preparations. This enzyme preparation contained N-acetyl-D-glucosaminidase and N,N'-diacetylchitobiohydrolase. N-Acetyl-D-glucosaminidase was inactive above 50 degrees C, but N,N'-diacetylchitobiohydrolase was stable at this temperature. Utilizing the temperature sensitivities of the chitin degradation enzymes in crude enzyme preparation, N-acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose [(GlcNAc)(2)] were selectively produced from chitin. At 45 degrees C, GlcNAc was produced as a major hydrolytic product (94% composition) with a yield of 74% in 5 d, meanwhile at 55 degrees C (GlcNAc)(2) was the major product (86%) with a yield of 35% within 5 d.
authors.affiliation: Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, Korea.
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Kuk
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Jung
authors.forename: Gyung
authors.initials: G
authors.lastname: Hyun Jo
authors.forename: Joon Seob
authors.initials: JS
authors.lastname: Ahn
authors.forename: Kil Yong
authors.initials: KY
authors.lastname: Kim
authors.forename: Ro Dong
authors.initials: RD
authors.lastname: Park
mesh_terms: D000117:Acetylglucosamine / Q000096:biosynthesis*; D000333:Aeromonas / Q000201:enzymology*; D002686:Chitin / Q000378:metabolism*; D004187:Disaccharides / Q000096:biosynthesis*; D005959:Glucosidases / Q000378:metabolism*; D006868:Hydrolysis
chemical_list: D004187:Disaccharides; D002686:Chitin; C010913:N,N-diacetylchitobiose; D005959:Glucosidases; C505019:N,N'-diacetylchitobiohydrolase, Aeromonas; D000117:Acetylglucosamine