title: Subunit exchange of polydisperse proteins: mass spectrometry reveals consequences of alphaA-crystallin truncation.
abstract: The small heat shock protein, alpha-crystallin, plays a key role in maintaining lens transparency by chaperoning structurally compromised proteins. This is of particular importance in the human lens, where proteins are exposed to post-translational modifications over the life-time of an individual. Here, we examine the structural and functional consequences of one particular modification of alphaA-crystallin involving the truncation of 5 C-terminal residues (alphaA(1-168)). Using novel mass spectrometry approaches and established biophysical techniques, we show that alphaA(1-168) forms oligomeric assemblies with a lower average molecular mass than wild-type alphaA-crystallin (alphaA(WT)). Also apparent from the mass spectra of both alphaA(WT) and alphaA(1-168) assemblies is the predominance of oligomers containing even numbers of subunits; interestingly, this preference is more marked for alphaA(1-168). To examine the rate of exchange of subunits between assemblies, we mixed alphaB crystallin with either alphaA(WT) or alphaA(1-168) and monitored in a real-time mass spectrometry experiment the formation of heteroligomers. The results show that there is a significant decrease in the rate of exchange when alphaA(1-168) is involved. These reduced exchange kinetics, however, have no effect upon chaperone efficiency, which is found to be closely similar for both alphaA(WT) and alphaA(1-168). Overall, therefore, our results allow us to conclude that, in contrast to mechanisms established for analogous proteins from plants, yeast, and bacteria, the rate of subunit exchange is not the critical parameter in determining efficient chaperone behavior for mammalian alphaA-crystallin.
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, United Kingdom.
authors.forename: J Andrew
authors.initials: JA
authors.lastname: Aquilina
authors.forename: Justin L P
authors.initials: JL
authors.lastname: Benesch
authors.forename: Lin Lin
authors.initials: LL
authors.lastname: Ding
authors.forename: Orna
authors.initials: O
authors.lastname: Yaron
authors.forename: Joseph
authors.initials: J
authors.lastname: Horwitz
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
mesh_terms: D002845:Chromatography; D006801:Humans; D007700:Kinetics; D008027:Light; D013058:Mass Spectrometry / Q000379:methods*; D018832:Molecular Chaperones / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012542:Scattering, Radiation; D013696:Temperature; D013997:Time Factors; D038202:alpha-Crystallin A Chain / Q000737:chemistry*
chemical_list: D018832:Molecular Chaperones; D038202:alpha-Crystallin A Chain