Comparison of high-speed counter-current chromatography instruments for the separation of the extracts of the seeds of Oroxylum indicum.
Analytical Milli high-speed counter-current chromatography (HSCCC) was used for the selection and optimization of the two-phase solvent system to separate flavonoids from the extracts of the seeds of Oroxylum indicum. The optimum solvent system obtained from Milli-CCC was also the best solvent system for preparative HSCCC and led to the successful separation of two crude flavonoids from the seeds of O. indicum by Lab/Prep (laboratory preparative) HSCCC using different sized coils. Four flavonoids were isolated by preparative HSCCC: baicalein-7-O-diglucoside (25.0 mg, 92% purity), baicalein-7-o-glucoside (50.4 mg; 95% purity), baicalein (75 mg; purity 98%) and chrysin (100 mg; purity 98%).
Chen
L J
LJ
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Chengdu, Sichuan 610041, China. Iijuanl7@hotmail.com
Song
H
H
Lan
X Q
XQ
Games
D E
DE
Sutherland
I A
IA
D029402:Bignoniaceae / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000295:instrumentation*; D005419:Flavonoids / Q000302:isolation & purification; D010936:Plant Extracts / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*
D005419:Flavonoids; D010936:Plant Extracts