[Influence of sargassum fusiforme polysaccharide on apoptosis of tumor cells].
OBJECTIVE
To study mechanism of antitumor activity of Sargassum Fusiforme Polysaccharide (SFPS).


METHODS
The effect on cell cycle and apoptosis was studied with flow cytometry (FCM). Intracellular calcium concentration [Ca2+]i was marked with Fluo-3/AM and measured with laser scanner confocal microscope (LSCM).


RESULTS
SFPS inhibited G0/G1 stage SGC-7901 from entering to S stage and increased APO%. The [Ca2+]i showed a transient rise and return to the original level. The concentration could be raised again by administering CaCl2.


CONCLUSIONS
The antitumor effect of SFPS seems to be accomplished through the apoptosis associated with the increase in intracellular calcium concentration. Intracellular stores release the calcium during its action.
Ji
Yu-bin
YB
Postdoctoral Programme, the Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China. jyb@hrbcu.edu.cn
Gao
Shi-yong
SY
Zhang
Xiu-juan
XJ
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D002122:Calcium Chloride / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D006801:Humans; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D011134:Polysaccharides; D002122:Calcium Chloride; D002118:Calcium