title: Mouse Na+/K+-ATPase beta1-subunit has a K+-dependent cell adhesion activity for beta-GlcNAc-terminating glycans.
abstract: A 48-kDa beta-N-acetylglucosamine (GlcNAc)-binding protein was isolated from mouse brain by GlcNAc-agarose column chromatography. The N-terminal amino acid residues showed the protein to be a mouse Na(+)/K(+)-ATPase beta1-subunit. When the recombinant FLAG-beta1-subunit expressed in Sf-9 cells was applied to a GlcNAc-agarose column, only the glycosylated 38- and 40-kDa proteins bound to the column. In the absence of KCl, little of the proteins bound to a GlcNAc-agarose column, but the 38- and 40-kDa proteins bound in the presence of KCl at concentrations above 1 mM. Immunohistochemical study showed that the beta1-subunit and GlcNAc-terminating oligosaccharides are at the cell contact sites. Inclusion of anti-beta1-subunit antibody or chitobiose in cell aggregation assays using mouse neural cells resulted in inhibition of cell aggregation. These results indicate that the Na(+)/K(+)-ATPase beta1-subunit is a potassium-dependent lectin that binds to GlcNAc-terminating oligosaccharides: it may be involved in neural cell interactions.
authors.affiliation: Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173-0015, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kitamura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikekita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sato
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Akimoto
authors.forename: Yasumaru
authors.initials: Y
authors.lastname: Hatanaka
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.forename: Mitsushi
authors.initials: M
authors.lastname: Inomata
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002448:Cell Adhesion; D004187:Disaccharides / Q000494:pharmacology; D006031:Glycosylation; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology*; D021122:Protein Subunits; D012685:Sepharose / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000737:chemistry / Q000502:physiology*
chemical_list: D004187:Disaccharides; D011134:Polysaccharides; D021122:Protein Subunits; C032438:chitobiose; D012685:Sepharose; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium; D000117:Acetylglucosamine