title: Relationships between toxin gene content and genetic background in nasal carried isolates of Staphylococcus aureus from Asturias, Spain.
abstract: Staphylococcus aureus recovered from nasal carriers, producers and non-producers (43 isolates each) of classical pyrogenic toxin superantigens (PTSAgs), were screened for 17 additional PTSAg-genes by PCR. Percentages of 88.4 and 65.1 were positive for some new enterotoxin-gene, and 76.7 and 55.8 for enterotoxin-gene-clusters (egc-like), respectively. The 86 isolates belonged to 17 toxin-genotypes (all eta-, etb-, etd-, see- and sep-negative), and generated 40 SmaI-genomic profiles that in a dendrogram of similarity (S0.7) clustered into nine lineages and 11 non-clustered branches. Correlations between classical PTSAgs and SmaI-lineages were established and egc-like groupings appeared dispersed in six lineages.
authors.affiliation: Departamento de Biología Funcional, Area de Microbiologia, Universidad de Oviedo, C/Julián Claveria no. 6, 33006-Oviedo, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Fueyo
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Mendoza
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alvarez
authors.forename: M Cruz
authors.initials: MC
authors.lastname: Martín
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D002353:Carrier State / Q000382:microbiology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005098:Exotoxins / Q000235:genetics*; D006801:Humans; D009666:Nose / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013030:Spain; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D018089:Superantigens / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D018089:Superantigens; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific