title: Dephosphorylation of eNOS on Thr495 after transient forebrain ischemia in gerbil hippocampus.
abstract: We investigated phosphorylation of endothelial nitric oxide synthase (eNOS) at two major sites, Ser1177 and Thr495, which has a critical role to control its activity, in the gerbil hippocampal microvasculature after transient forebrain ischemia. Ser1177 phosphorylation was unchanged by 24 h after reperfusion, despite post-ischemic up-regulation of eNOS protein. However, Thr495 phosphorylation significantly and persistently decreased by 48 h. We here defined the changes in eNOS phosphorylation in vivo following brain ischemia/reperfusion. (ischemia).
authors.affiliation: Department of Neurosurgery, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto City, Kumamoto, Japan. 000m9033@med.stud.kumamoto-u.ac.jp
authors.forename: Akihito
authors.initials: A
authors.lastname: Hashiguchi
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Yano
authors.forename: Motohiro
authors.initials: M
authors.lastname: Morioka
authors.forename: Junichiro
authors.initials: J
authors.lastname: Hamada
authors.forename: Masato
authors.initials: M
authors.lastname: Kochi
authors.forename: Kohji
authors.initials: K
authors.lastname: Fukunaga
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III; D010766:Phosphorylation; D015424:Reperfusion / Q000379:methods; D013912:Threonine / Q000378:metabolism*; D013997:Time Factors
chemical_list: D013912:Threonine; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III