Rapid isolation of Brachyspira hyodysenteriae and Brachyspira pilosicoli from pigs.
The aim of this study was to compare and evaluate the time required to isolate Brachyspira hyodysenteriae and Brachyspira pilosicoli from porcine faeces. This was done using previously described selective media (spectinomycin) S400, (colistin, vancomycin and spectinomycin) CVS and (spectinomycin, vancomycin, colistin, spiramycin and rifampin with swine faecal extract) BJ, compared with the method based on blood agar modified medium, with spectinomycin and rifampin (BAM-SR), including a pre-treatment step. Fourteen spirochaetal strains were obtained in pure cultures after 5 days (48 h in BAM-SR primary plate and three passages every 24 h in brain heart infusion (BHI) without antibiotics) pre-treating simulated samples in brain heart infusion broth with spectinomycin (400 microg/ml) and rifampin (15 microg/ml), before streaking on the selective BAM-SR medium. Spirochaetes from samples in S400, CVS and BJ, with and without pre-treatment, were obtained in pure cultures only after repeatedly transferring on plates of the same selective medium requiring 15-18 days according to the strain. BAM-SR used after the pre-treatment step showed a detection limit ranging from 3.5 x 10(2) to 6.7 x 10(7) cells/g faeces and was the only method able to support the growth of spirochaetes after 48 h.
Calderaro
A
A
Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Viale A. Gramsci, 14-43100 Parma, Italy. micromed@unipr.it
Bommezzadri
S
S
Piccolo
G
G
Zuelli
C
C
Dettori
G
G
Chezzi
C
C
D000818:Animals; D015169:Colony Count, Microbial / Q000662:veterinary; D003470:Culture Media / Q000737:chemistry; D004403:Dysentery / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D012293:Rifampin; D000198:Spectinomycin; D013144:Spirochaetales / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology; D013997:Time Factors
D003470:Culture Media; D000198:Spectinomycin; D012293:Rifampin