Direct somatic embryogenesis and plant regeneration from leaf, petiole, and stem explants of Golden Pothos.
Somatic embryos directly formed at cut edges or on the surface of leaf explants, around cut ends or along side surfaces of petiole and stem explants of 'Golden Pothos' [Epipremnum aureum (Linden & Andre) Bunt.] on Murashige and Skoog (MS) medium supplemented with N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU) or N-phenyl-N'-1, 2, 3-thiadiazol-5-ylurea (TDZ) with alpha-naphthalene acetic acid (NAA) and a medium called MK containing MS salts with Kao's vitamins, supplemented with 2.0 mg/l TDZ and 0.2 mg/l NAA. Somatic embryos were also produced on MS medium containing 2.0 mg/l kinetin (KN) and 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) from leaf and petiole explants, MS medium supplemented with 2.0 mg/l CPPU and 0.5 mg/l 2,4-D from petiole and stem explants, and 2.0 mg/l TDZ and 0.2 mg/l or 0.5 mg/l 2,4-D from stem explants. In addition, somatic embryos occurred from stem explants on Chu's N6 medium containing 2.0 mg/l CPPU and 0.2 mg/l NAA. Somatic embryos matured and grew into multiple buds, shoots, or even plantlets after 2-3 months on the initial culture medium. Germination was optimal on MS medium containing either 2 mg/l 6-benzylaminopurine (BA) and 0.2 mg/l NAA or 2 mg/l zeatin and 0.2 mg/l NAA. Shoots elongated better and roots developed well on MS medium with no growth regulators. Approximately 30-100 plantlets were regenerated from each explant. The regenerated plants grew vigorously after transplanting to a soil-less container substrate in a shaded greenhouse.
Zhang
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Q
Environmental Horticulture Department and Mid-Florida Research and Education Center, IFAS, University of Florida, 2725 Binion Road, Apopka, FL 32703, USA.
Chen
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J
Henny
R J
RJ
D029064:Araceae / Q000196:embryology*; D003470:Culture Media; D018525:Germination / Q000502:physiology*; D010671:Phenylurea Compounds / Q000737:chemistry; D010937:Plant Growth Regulators / Q000737:chemistry; D018515:Plant Leaves / Q000196:embryology; D018520:Plant Shoots / Q000196:embryology; D018547:Plant Stems / Q000196:embryology; D012038:Regeneration / Q000502:physiology
D003470:Culture Media; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators