Is hypothermia a stress condition in HepG2 cells? Expression and localization of Hsp70 in human hepatoma cell line.
To understand hypothermia as a stress condition we determined the expression and localization of Hsp70 under hyperthermic and hypothermic stress in human hepatoma HepG2 cells. Western blot analysis indicates that there was a statistically significant increase of Hsp70 expression under thermal stresses. Immunohistochemically, the distribution of inducible Hsp70 in stressed cells showed a granular pattern mostly in the cytoplasm. At subcellular level, Hsp70 was localized in the nucleus, vacuoles, cytoskeletal components and dispersed throughout the cytoplasm. Accumulation of Hsp70 in cells under hypothermia could be related to restitution of cell equilibrium modified by this thermal stress condition. The protective effect of hypothermia could be associated with promotion of Hsp expression. We suggest that hypothermia is a stress capable of inducing Hsp70 expression in human HepG2 cells.
Rada
Alegna
A
Sección de Biología Celular, Instituto de Medicina Tropical, Apdo 47019, Facultad de Medicina, Universidad Central de Venezuela, Caracas 1041A, Venezuela.
Tonino
Paola
P
Anselmi
Guillermo
G
Strauss
Mirian
M
D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D007035:Hypothermia / Q000503:physiopathology*; D007124:Immunoenzyme Techniques; D046529:Microscopy, Electron, Transmission
D018840:HSP70 Heat-Shock Proteins