Nanoparticle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease.
The recently developed ultrasensitive bio-barcode assay was used to measure the concentration of amyloid-beta-derived diffusible ligands (ADDLs), a potential soluble pathogenic Alzheimer's disease (AD) marker, in the cerebrospinal fluid (CSF) of 30 individuals. ADDL concentrations for the subjects diagnosed with AD were consistently higher than the levels in the CSF taken from nondemented age-matched controls. Studies of ADDLs or for any other potential pathogenic AD markers in CSF have not been possible because of their low concentration in CSF (<1 pM). This study is a step toward a diagnostic tool, based on soluble pathogenic markers for the debilitating disease.
Georganopoulou
Dimitra G
DG
Department of Chemistry and Institute of Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
Chang
Lei
L
Nam
Jwa-Min
JM
Thaxton
C Shad
CS
Mufson
Elliott J
EJ
Klein
William L
WL
Mirkin
Chad A
CA
D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000235:genetics; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000235:genetics; D001483:Base Sequence; D015415:Biomarkers / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics; D006801:Humans; D008024:Ligands; D036103:Nanotechnology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012995:Solubility
D016229:Amyloid beta-Peptides; D015415:Biomarkers; D008024:Ligands; D004247:DNA