title: [Protective effects of acidic fibroblast growth factor on intestinal ischemia/reperfusion in rats].
abstract: OBJECTIVE
  To observe the change in hepatic and renal functions, change in the plasma D-lactate level, and the expression of proliferating cell nuclear antigen (PCNA) after intestinal I/R injury, so as to explore the effects of reconstructive human acid fibroblast growth factor(aFGF) on intestinal I/R injury in rats.
  
  
  METHODS
  One hundred and twenty-six Wistar rats were divided into sham-operated, ischemia (45 minutes) plus reperfusion, reconstructive human aFGF treatment (2, 4, 8 microg aFGF) and wild type aFGF(2, 6, 12, and 24 hours, respectively) groups. Hepatic and renal functions and the levels of plasma D-lactate were determined and the expression of PCNA was assessed.
  
  
  RESULTS
  Compared with all other groups, bowel barrier function and hepatic and renal functions showed most marked deterioration in sham-operated group. The damages were less marked in reconstructive human aFGF group compared with other groups 24 hours after ischemia/reperfusion of the intestine, and the protective effect was best shown when 4 microg of aFGF was given. The trend of expression of PCNA was similar to that of changes in D-lactate level.
  
  
  CONCLUSIONS
  Wild type reconstructive human aFGF treatment significantly improves the outcome of ischemia/reperfusion injury to the intestine, and the effect is dose-dependent.
authors.affiliation: 304th Clinical Department of General Hospital of PLA, Beijing 100037, China.
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Weng
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Fu
authors.forename: Xiu-xia
authors.initials: XX
authors.lastname: Li
authors.forename: Tong-zhu
authors.initials: TZ
authors.lastname: Sun
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Jun-you
authors.initials: JY
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D016220:Fibroblast Growth Factor 1; D019344:Lactic Acid