title: Decreased expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in the kidneys of hereditary nephrotic (ICGN) mice.
abstract: Matrix metalloporoteinases (MMPs), which are dominantly regulated by tissue inhibitors of metalloproteinase (TIMPs), play important roles in extracellular matrix (ECM) degradation and are involved in the progression of kidney diseases. In glomeruli and tubulointerstitum of hereditary nephrotic (ICR-derived glomerulonephritis: ICGN) mouse kidneys, hyper-accumulation of ECM components occurred, and MMP activity decreased. In the present study, because lower levels of MMP activity may contribute to the progression of renal fibrosis in ICGN mice, Western blotting analysis and immunohistochemical staining for MMPs and TIMPs were performed to verify the expression levels of these proteins. Levels of MMP-2, MMP-9, MT1-MMP, TIMP-1 and TIMP-2 in the kidneys were decreased in ICGN mice in comparison with normal ICR mice. These results indicate that small amounts and low levels of activity of MMPs cause the progression of renal fibrosis in ICGN mice.
authors.affiliation: Department of Veterinary Sciences, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Kozue
authors.initials: K
authors.lastname: Uchio-Yamada
authors.forename: Noboru
authors.initials: N
authors.lastname: Manabe
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Goto
authors.forename: Sayuri
authors.initials: S
authors.lastname: Anann
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takano
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
authors.forename: Junichiro
authors.initials: J
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005786:Gene Expression Regulation; D005921:Glomerulonephritis / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D009401:Nephrosis / Q000235:genetics / Q000378:metabolism*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis* / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020782:Matrix Metalloproteinases