Prevention of flower development in birch and other plants using a BpFULL1::BARNASE construct.
The prevention of flower formation is important for avoiding the spread of transgenes from genetically modified plants into wild populations. Moreover, the resources not expended for the generation of flowers and fruits might be allocated to increased vegetative growth. We have been developing methods for preventing flower formation in silver birch (Betula pendula), a tree species of considerable economical importance in the boreal region. Here we study the suitability of the promoter of BpFRUITFULL-LIKE1 (BpFULL1, formerly BpMADS5) for tissue-specific ablation of inflorescences in Arabidopsis, tobacco and birch. With all these species, the development of inflorescences was successfully prevented. The results show that the BpFULL1::BARNASE construct has potential biotechnological applications in different plant species.
Lännenpää
M
M
Department of Biology, University of Joensuu, P.O. Box 111, 80101 Joensuu, Finland. mika.lannenpaa@joensuu.fi
Hassinen
M
M
Ranki
A
A
Hölttä-Vuori
M
M
Lemmetyinen
J
J
Keinonen
K
K
Sopanen
T
T
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins; D029662:Betula / Q000235:genetics* / Q000254:growth & development; D035264:Flowers / Q000235:genetics* / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006131:Growth Inhibitors / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012260:Ribonucleases / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development
D001426:Bacterial Proteins; D006131:Growth Inhibitors; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease