[Determination of scutellarin and rutin in Shenfukang capsules by reversed-phase high performance liquid chromatography].
A rapid and simple method for the determination of scutellarin and rutin in Shenfukang capsules has been developed using reversed-phase high performance liquid chromatography. Scutellarin and rutin were first extracted by ultrasonic extraction with methanol-water (1:1, v/v). The resulting extracts were separated on a C18 column (4.6 mm i.d. x 150 mm, 5 microm). The mobile phase consisting of 0.02 mol/L KH2PO4 solution containing 1.0% glacial acetic acid-methanol (63:37, v/v) was run at a flow rate of 1.0 mL/min. The detection wavelength was 330 nm and the injection volume was 20 microL. Good linearity in the range of 10 mg/L to 300 mg/L was obtained for scutellarin and rutin. The method was successfully used for the quantification of scutellarin and rutin of Shenfukang capsules.
Zhang
Wenzhu
W
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China.
Liu
Xia
X
Wen
Bo
B
Jiang
Shengxiang
S
D047310:Apigenin / Q000032:analysis*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D012431:Rutin / Q000032:analysis*; D031363:Scutellaria / Q000737:chemistry
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012431:Rutin; D047310:Apigenin; C458179:scutellarein