[A comparative study of blood culture conventional method vs. a modified lysis/centrifugation technique for the diagnosis of fungemias].
The purpose of this work was to compare the efficacy of blood culture conventional method vs. a modified lysis/centrifugation technique. Out of 450 blood specimens received in one year, 100 where chosen for this comparative study: 60 from patients with AIDS, 15 from leukemic patients, ten from febrile neutropenic patients, five from patients with respiratory infections, five from diabetics and five from septicemic patients. The specimens were processed, simultaneously, according to the above mentioned methodologies with daily inspections searching for fungal growth in order to obtain the final identification of the causative agent. The number (40) of isolates recovered was the same using both methods, which included; 18 Candida albicans (45%), ten Candida spp. (25%), ten Histoplasma capsulatum (25%), and two Cryptococcus neoformans (5%). When the fungal growth time was compared by both methods, growth was more rapid when using the modified lysis/centrifugation technique than when using the conventional method. Statistical analysis revealed a significant difference (p<0.05) between them. The modified lysis/centrifugation technique showed to be more efficacious than the conventional one, and therefore the implementation of this methodology is highly recommended for the isolation of fungi from blood.
Santiago
Axel Rodolfo
AR
Laboratorio de Micología, Hospital Universitario de Caracas, Caracas, Venezuela. axelsantiago@cantv.net
Hernández
Betsy
B
Rodríguez
Marina
M
Romero
Hilda
H
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000150:complications; D001769:Blood / Q000382:microbiology*; D001773:Blood Cells / Q000187:drug effects; D002175:Candida / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000382:microbiology; D002498:Centrifugation; D003453:Cryptococcosis / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000302:isolation & purification; D048909:Diabetes Complications / Q000097:blood; D016469:Fungemia / Q000150:complications / Q000175:diagnosis / Q000382:microbiology*; D005658:Fungi / Q000254:growth & development / Q000302:isolation & purification*; D006658:Histoplasma / Q000254:growth & development / Q000302:isolation & purification; D006660:Histoplasmosis / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000150:complications; D009172:Mycology / Q000379:methods*; D009503:Neutropenia / Q000097:blood / Q000150:complications; D012141:Respiratory Tract Infections / Q000097:blood / Q000150:complications / Q000382:microbiology; D012503:Saponins / Q000494:pharmacology; D013048:Specimen Handling / Q000379:methods*
D012503:Saponins