[Study on experimental factors for extraction of alkaloids from Aconitum carmichaili].
OBJECTIVE
To determine the suitable conditions for extracting alkaloidals from Aconitum carmichaili.


METHODS
Using methods in Chinese Pharmacopeia for determining yields of extraction, which include spectrometry and single-factor tests, we investigated vavious factors that would affect the extraction of alkaloids from Aconiti.


RESULTS
The suitable extractant was 85% ethanol, starting material was medium-size powder of aconiti, 10 times of the volume of the solvent was used in extraction for twice 1.75 h each time. The extraction yield was 1.21%, with a content of 0.33% alkaloids.


CONCLUSIONS
Extraction conditions determined with single factor test was consistent and feasible.
Liang
Chun-mei
CM
Pharmaceutical Department, People's Hospital of Hezhou, Hezhou 542800, China.
Yan
Ru-qing
RQ
D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol; D010316:Particle Size; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
D000470:Alkaloids; D011208:Powders; D000431:Ethanol