Rice Na+/H+-antiporter Nhx1 partially complements the alkali-metal-cation sensitivity of yeast strains lacking three sodium transporters.
A triple mutant strain of Saccharomyces cerevisiae lacking its own Na+-ATPases and Na+/H+ antiporters (enal-4delta nha1delta nhx1delta) was used for the expression of the Oryza sativa NHX1 gene encoding a putative vacuolar Na+/H+ exchanger. Upon expression in yeast cells, the OsNhx 1p is not a transport system specific only for sodium cations but it has a broad substrate specificity for at least four alkali metal cations (Na+, Li+, K+ and Rb+) and is able to substitute for the endogenous yeast ScNhx1 antiporter. Its activity contributes to sequestration of alkali metal cations in intracellular vesicles.
Kinclová-Zimmermannová
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Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czechia.
Flegelová
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Sychrová
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D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D005800:Genes, Fungal; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008670:Metals / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
D018744:DNA, Plant; D008670:Metals; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D017923:Sodium-Hydrogen Exchangers