Low-temperature growth of Shewanella oneidensis MR-1.
Shewanella oneidensis MR-1 is a mesophilic bacterium with a maximum growth temperature of approximately 35 degrees C but the ability to grow over a wide range of temperatures, including temperatures near zero. At room temperature ( approximately 22 degrees C) MR-1 grows with a doubling time of about 40 min, but when moved from 22 degrees C to 3 degrees C, MR-1 cells display a very long lag phase of more than 100 h followed by very slow growth, with a doubling time of approximately 67 h. In comparison to cells grown at 22 degrees C, the cold-grown cells formed long, motile filaments, showed many spheroplast-like structures, produced an array of proteins not seen at higher temperature, and synthesized a different pattern of cellular lipids. Frequent pilus-like structures were observed during the transition from 3 to 22 degrees C.
Abboud
Randa
R
Department of Biological Sciences, 3651 Trousdale Pkwy., AHF 107, University of Southern California, Los Angeles, CA 90089-0371, USA.
Popa
Radu
R
Souza-Egipsy
Virginia
V
Giometti
Carol S
CS
Tollaksen
Sandra
S
Mosher
Jennifer J
JJ
Findlay
Robert H
RH
Nealson
Kenneth H
KH
D001426:Bacterial Proteins / Q000032:analysis; D003080:Cold Temperature; D008055:Lipids / Q000032:analysis; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D020592:Shewanella / Q000737:chemistry / Q000254:growth & development* / Q000648:ultrastructure
D001426:Bacterial Proteins; D008055:Lipids