A new approach to pharmacological effects on ciliary beat frequency in cell cultures--exemplary measurements under Pelargonium sidoides extract (EPs 7630).
The ciliary beat frequency (CBF) is an important parameter of the defence mechanism of the mucociliary system. We present a new method to determine pharmacological effects on CBF in vitro. Ciliated cell cultures of human nasal epithelium were obtained from partial resection of hyperplastic inferior turbinates in rhinosurgery. An adherent monolayer culture of ciliated cells was present after 10 days in vitro. This study exemplary examines, if a special extract from the roots of Pelargonium sidoides (EPs 7630) has an effect on the CBF in vitro. The influence of three concentrations of the extract (1, 30, 100 microg/ml) was tested. EPs 7630 significantly and concentration-dependently increased CBF to 123% at 30 microg/ml and to 133% at 100 microg/ml compared to the equilibration phase (100%). After rinsing with extract-free medium the CBF of cultured cells returned to nearly the normal range. In future, drug manipulation of the CBF by local application of rhinologics could be a new therapeutical concept in the treatment of upper airway diseases.
Neugebauer
P
P
ENT-Department, University of Cologne, Cologne, Germany. hno.neugebauer@uni-koeln.de
Mickenhagen
A
A
Siefer
O
O
Walger
M
M
D000316:Adrenergic alpha-Agonists / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects* / Q000502:physiology; D003371:Cough / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009079:Mucociliary Clearance / Q000187:drug effects*; D009297:Nasal Mucosa / Q000166:cytology / Q000187:drug effects; D031316:Pelargonium; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots
D000316:Adrenergic alpha-Agonists; C476332:EPs 7630; D010936:Plant Extracts