[Studies on influence of fungal elicitor on hairy root of Panax ginseng biosynthesis ginseng saponin and biomass].
OBJECTIVE
To study influence of fungal elicitors on the biomass and ginseng saponin biosynthesis of hairy roots of Panax ginseng (HRPG).


METHODS
Fungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV-spectrophotometry and RP-HPLC.


RESULTS
Fungal elicitors coula not only can influence on HRPG biomass and total ginseng sponin, but also improve or decrease some monomeric sponin. The total ginseng sponin could be increased to 3.649% but Rg1 and Re could not be detected when A. niger elicitors wss 20 mg x L(-1) in the culture fluid.


CONCLUSIONS
Fungal elicitor has specificity influence on secondary metabolite of HRPG. HRPG can biosynthesize specially active component by using specific fungal elicitor is used.
Liu
Jun
J
School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210038, China. firejj217@hotmail.com
Ding
Jia-yi
JY
Zhou
Qian-yun
QY
He
Lin
L
Wang
Zheng-tao
ZT
D001234:Aspergillus niger / Q000737:chemistry / Q000502:physiology; D018920:Coculture Techniques; D005670:Fusarium / Q000737:chemistry / Q000502:physiology*; D036145:Ginsenosides / Q000096:biosynthesis*; D005894:Panax / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology
D036145:Ginsenosides; D011134:Polysaccharides; C049864:ginsenoside Re; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1