Immobilization of single-stranded DNA by self-assembled polymer on gold substrate for a DNA chip.
We developed a self-assembling polymer based on polyallylamine (PAH) for use in DNA chips. Thioctic acid (TA) was covalently attached to PAH in sidechains to immobilize the polymer on a gold surface by self-assembly. N-hydroxysuccinimide-ester terminated probe single-stranded (ss) DNA is easily covalently immobilized onto a TA-PAH-coated gold surface. Finally, the surface was covered with polyacrylic acid, which formed ion complexes with the TA-PAH, to reduce the cationic charge. This ssDNA on a polymer-coated surface recognized a fully matched DNA sequence and restrained nonspecific adsorption of target DNA. The selectivity and efficiency of hybridization was affected by adjusting the ionic strength of sodium chloride.
Taira
Shu
S
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 4, 1-1-1 Higashi, Tsukuba 305-8562, Japan.
Yokoyama
Kenji
K
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D009994:Osmolar Concentration; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012964:Sodium / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013696:Temperature; D008063:Thioctic Acid / Q000737:chemistry
D004277:DNA, Single-Stranded; D015345:Oligonucleotide Probes; D011073:Polyamines; D011108:Polymers; D008063:Thioctic Acid; D006046:Gold; D012964:Sodium