Increases in extracellular fluid glucose levels in the rat hippocampus following an anesthetic dose of pentobarbital or ketamine-xylazine: an in vivo microdialysis study.
Using in vivo microdialysis, we examined glucose levels in the extracellular fluid (ECF) of the hippocampus and in the blood prior to and during pentobarbital (45 mg/kg) or ketamine-xylazine (66 mg/kg, 7 mg/kg) anesthesia. Anesthesia with either pentobarbital or ketamine-xylazine significantly increased hippocampal ECF glucose levels (mean peak increases of +71% and +85%, respectively). In addition, there were substantial increases in blood glucose levels (mean peak increases of +24% and +30%, respectively). The increased levels of hippocampal ECF glucose during anesthesia complement past evidence for decreases in ECF glucose in the hippocampus observed while rats perform a memory task sensitive to hippocampal damage, providing further support for the view that ECF glucose levels in the hippocampus are dynamically coupled to local neural activity.
Canal
Clinton E
CE
The Neuroscience Program, University of Illinois, Champaign, IL 61820, USA.
McNay
Ewan C
EC
Gold
Paul E
PE
D000700:Analgesics / Q000494:pharmacology*; D000768:Anesthesia, General / Q000379:methods*; D019162:Anesthetics, Combined / Q000008:administration & dosage; D000818:Animals; D005110:Extracellular Space / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D017551:Microdialysis / Q000379:methods; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014991:Xylazine / Q000494:pharmacology
D000700:Analgesics; D019162:Anesthetics, Combined; D014991:Xylazine; D007649:Ketamine; D010424:Pentobarbital; D005947:Glucose