[Micellar electrokinetic capillary chromatographic separation of amino acids derivatized with 2-(9-carbazole)-ethyl chloroformate as a pre-column labeling reagent].
2-(9-Carbazole)-ethyl chloroformate (CEOC) has been used for the pre-column derivatization of amino acids in micellar electrokinetic capillary chromatography (MEKC). Nine amino acid standards (Ser, Thr, Gly, Glu, Asp, Val, Ile, Leu, Phe) were labeled with CEOC and separated in a buffer system containing 20 mmol/L borate-30 mmol/L sodium lauryl sulfate (SDS) and 3% acetonitrile at pH 9.0 with an applied voltage of 18 kV and column temperature of 25 degrees C. The derivatives were detected at UV 214 nm. Under optimal conditions, this method offers a baseline separation for nine CEOC-derivatized amino acids within 14 min. The linearity range was from 0.025 to 0.25 mmol/L and the detection limits were from 2.15 to 2.46 micromol/L.
Zhang
Lin
L
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China.
You
Jinmao
J
Ping
Guichen
G
Zhang
Weibing
W
Yan
Chao
C
Zhang
Yukui
Y
D000596:Amino Acids / Q000302:isolation & purification*; D002227:Carbazoles / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*
D000596:Amino Acids; D002227:Carbazoles