title: Stretched exponential decay and correlations in the catalytic activity of fluctuating single lipase molecules.
abstract: Single-molecule techniques offer a unique tool for studying the dynamical behavior of individual molecules and provide the possibility to construct distributions from individual events rather than from a signal stemming from an ensemble of molecules. In biological systems, known for their complexity, these techniques make it possible to gain insights into the detailed spectrum of molecular conformational changes and activities. Here, we report on the direct observation of a single lipase-catalyzed reaction for extended periods of time (hours), by using confocal fluorescence microscopy. When adding a profluorescent substrate, the monitored enzymatic activity appears as a trajectory of "on-state" and "off-state" events. The waiting time probability density function of the off state and the state-correlation function fit stretched exponentials, independent of the substrate concentration in a certain range. The data analysis unravels oscillations in the logarithmic derivative of the off-state waiting time probability density function and correlations between off-state events. These findings imply that the fluctuating enzyme model, which involves a spectrum of enzymatic conformations that interconvert on the time scale of the catalytic activity, best describes the observed enzymatic activity. Based on this model, values for the coupling and reaction rates are extracted.
authors.affiliation: School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel.
authors.forename: Ophir
authors.initials: O
authors.lastname: Flomenbom
authors.forename: Kelly
authors.initials: K
authors.lastname: Velonia
authors.forename: Davey
authors.initials: D
authors.lastname: Loos
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Masuo
authors.forename: Mircea
authors.initials: M
authors.lastname: Cotlet
authors.forename: Yves
authors.initials: Y
authors.lastname: Engelborghs
authors.forename: Johan
authors.initials: J
authors.lastname: Hofkens
authors.forename: Alan E
authors.initials: AE
authors.lastname: Rowan
authors.forename: Roeland J M
authors.initials: RJ
authors.lastname: Nolte
authors.forename: Mark
authors.initials: M
authors.lastname: Van der Auweraer
authors.forename: Frans C
authors.initials: FC
authors.lastname: de Schryver
authors.forename: Joseph
authors.initials: J
authors.lastname: Klafter
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D002175:Candida / Q000201:enzymology; D005656:Fungal Proteins; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D008954:Models, Biological; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D005656:Fungal Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica