The role of antioxidant supplementation in cardiac transplantation: an experimental study in rats.
BACKGROUND
We investigated three antioxidants, inositol hexaphosphate (IP6), superoxide dismutase (SOD), and catalase (CAT), using a Langendorf model of heart transplantation.


METHODS
Rat hearts were mounted on a Langendorf perfusion apparatus with addition of IP6, SOD+CAT, IP6+SOD+CAT to St. Thomas Hospital solution (n=6 for each) versus a control group (n=6), not containing supplementation. Global ischemia was achieved for 6 hours.


RESULTS
The worst peak-to-peak (PP) and +dp/dt maximum values were observed in the IP6+SOD+CAT group, the values being significantly lower than those in the SOD+CAT group. The lowest plasma creatine kinase (CK), CK-muscle-band (CK-MB), and lactate dehydrogenase levels were measured from the SOD+CAT group. The highest values for CK were in the control group, and those for CK-MB were in the IP6 group. The lowest myocardial malondialdehyde and adenosine triphosphate values were observed in the SOD+CAT group.


CONCLUSIONS
Supplementing St. Thomas Hospital solution with IP6 did not ameliorate myocardial damage following global ischemia. The contractility deteriorated further when IP6+SOD+CAT were used together; however, SOD+CAT improved cardiac mechanical functions, and significantly reduced myocardial damage.
Kazimoglu
K
K
Department of Cardiovascular Surgery, SureyyapasaThoracic and Cardiovascular Disease Hospital, Istanbul, Turkey.
Bozkurt
A K
AK
Suzer
O
O
Konukoglu
D
D
Koksal
C
C
Kurdal
T
T
Turhan
M S
MS
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D016027:Heart Transplantation / Q000502:physiology*; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D007295:Inositol Phosphates; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D002374:Catalase; D013482:Superoxide Dismutase