Effects of antineoplaston AS2-1 against post-operative lung metastasis in orthotopically implanted colon cancer in nude rat.
We have investigated the efficacy and mechanisms of antineoplaston AS2-1 against post-operative lung metastasis following removal of implanted human colon cancer in nude rat. The influence of AS2-1 on in vitro KM12SM human colon carcinoma cell activities (growth, cell cycle, and apoptosis) was evaluated. AS2-1 was administered perorally after removal of the implanted KM12SM cecal cancer in nude rat. AS2-1 inhibited KM12SM cell proliferation through G1 cell arrest and, at a higher concentration, induction of apoptosis. AS2-1 showed significant reduction in lung metastasis at 5 weeks after cecal removal. The survival rate in the AS2-1 group was significantly higher than that in the control. TUNEL staining on the lung metastatic tumors revealed that the apoptosis index (AI) in the AS2-1 group was significantly higher. Antineoplaston AS2-1 showed an antimetastatic effect against post-operative lung metastases from colon cancer through G1 cell arrest and the subsequent induction of apoptosis.
Matono
Keiko
K
Department of Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume City, Fukuoka 830-0011, Japan.
Ogata
Yutaka
Y
Tsuda
Hideaki
H
Araki
Yasumi
Y
Shirouzu
Kazuo
K
D000818:Animals; D002277:Carcinoma / Q000517:prevention & control* / Q000556:secondary* / Q000662:veterinary; D003110:Colonic Neoplasms / Q000473:pathology* / Q000601:surgery* / Q000662:veterinary; D004338:Drug Combinations; D016193:G1 Phase / Q000187:drug effects; D005973:Glutamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D008175:Lung Neoplasms / Q000517:prevention & control* / Q000556:secondary* / Q000662:veterinary; D008297:Male; D009374:Neoplasms, Experimental; D010648:Phenylacetates / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D004338:Drug Combinations; D010648:Phenylacetates; D005973:Glutamine; C092038:antineoplaston AS 2-1