Differential gene expression in WKY and SHR brain following acute and chronic air-puff stress.
This study determined the expression levels of tyrosine hydroxylase (TH), arginine vasopressin (AVP), and prepro-neuropeptide Y (NPY) mRNA in the brain following acute and chronic air-puff stress. Acute stress increased TH gene expression in the locus coeruleus of Wistar-Kyoto (WKY) rats (+80%), but only modestly in spontaneously hypertensive rats (SHR; +19%). Hypothalamic AVP mRNA in SHR was persistently elevated in the supraoptic nucleus (+24% acute; +19% chronic), but reduced in the magnocellular region of the paraventricular nucleus (-15% acute; -20% chronic). In the arcuate nucleus, NPY mRNA expression increased by 60% and 81% in response to acute and chronic air-puff stress, respectively, in SHR. These data suggest differential adaptation to air-puff stress between WKY and SHR.
McDougall
Stuart J
SJ
Department of Pharmacology, Monash University, Victoria 3800, Australia.
Widdop
Robert E
RE
Lawrence
Andrew J
AJ
D000704:Analysis of Variance; D000818:Animals; D001127:Arginine Vasopressin / Q000235:genetics / Q000378:metabolism*; D001345:Autoradiography; D015870:Gene Expression / Q000502:physiology*; D007031:Hypothalamus / Q000033:anatomy & histology / Q000378:metabolism*; D017403:In Situ Hybridization / Q000379:methods; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism*; D010812:Physical Stimulation / Q000379:methods; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism*
D009478:Neuropeptide Y; D011498:Protein Precursors; D012333:RNA, Messenger; D001127:Arginine Vasopressin; C042084:preproneuropeptide Y; D014446:Tyrosine 3-Monooxygenase