Occurrence, hosts, morphology, and molecular characterisation of Pasteuria bacteria parasitic in nematodes of the family Plectidae.
Parasitic bacteria of the genus Pasteuria are reported for three Anaplectus and four identified and several unidentified Plectus species found in eight countries in various habitats. The pasteurias from plectids agree in essential morphological characters of sporangia and endospores as well as in developmental cycle with those of the Pasteuria species and strains described from tylenchid nematodes, but appear to be mainly distinguished from these by absence of a distinct perisporium in the spores and the endospores obviously not being cup- or saucer-shaped. The wide range of measurements and morphological peculiarities of sporangia and endospores suggest that probably several Pasteuria species have to be distinguished as parasites in Plectidae. From an infected juvenile of an unidentified plectid species the 16S rRNA gene sequence of Pasteuria sp. was obtained. Substantial sequence divergence from described Pasteuria species and its phylogenetic position on molecular trees indicate that this Pasteuria sp. could be considered as a new species. Preliminary results of the analysis of DNA phylogeny of Pasteuria spp. and their nematode hosts provide evidence for incongruence of their phylogenetic history and of host switching events during evolution of the bacterial parasites.
Sturhan
Dieter
D
Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Nematologie und Wirbeltierkunde, Toppheideweg 88, 48161 Münster, Germany.
Shutova
Tatyana S
TS
Akimov
Vladimir N
VN
Subbotin
Sergei A
SA
D000818:Animals; D018407:Bacterial Physiological Phenomena; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification* / Q000502:physiology*; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D009348:Nematoda / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S