title: An isocratic ion exchange HPLC method for the simultaneous determination of flucloxacillin and amoxicillin in a pharmaceutical formulation for injection.
abstract: An isocratic ion exchange high performance liquid chromatography method was developed for the simultaneous determination of flucloxacillin and amoxicillin in pharmaceutical formulations for injections. The separation was made by a ZORBAX 300-SCX column using 0.025 M ammonium dihydrogen phosphate (adjusted to pH 2.6 with phosphoric acid)-acetonitrile (95:5) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limits of quantification were approximately 0.2 microg/ml for each drug. Due to its simplicity and accuracy the method is particularly suitable for routine pharmaceutical quality control.
authors.affiliation: Division of Antibiotics, Shanghai Institute for Drug Control, Liuzhou Road 615, Shanghai 200233, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Hongwu
authors.initials: H
authors.lastname: Wang
authors.forename: V Bruce
authors.initials: VB
authors.lastname: Sunderland
mesh_terms: D000658:Amoxicillin / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004338:Drug Combinations; D005436:Floxacillin / Q000302:isolation & purification*; D007267:Injections; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004338:Drug Combinations; D004364:Pharmaceutical Preparations; D005436:Floxacillin; D000658:Amoxicillin