Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum.
Based on the intergenic sequences of Tri5-Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of Fusarium graminearum. Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 F. graminearum isolates from 12 provinces of China showed that 310 were DON-chemotypes and 54 were NIV-chemotypes. Sequence analyses revealed that DON-chemotypes display more variations than NIV-chemotypes. This PCR assay could be used to detect mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody-based mycotoxin tests.
Li
He-Ping
HP
Molecular Biotechnology Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Wu
Ai-Bo
AB
Zhao
Chun-Sen
CS
Scholten
Olga
O
Löffler
Huub
H
Liao
Yu-Cai
YC
D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D002523:Edible Grain / Q000382:microbiology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005670:Fusarium / Q000737:chemistry / Q000145:classification*; D009183:Mycotoxins / Q000737:chemistry* / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D014255:Trichothecenes / Q000737:chemistry* / Q000378:metabolism
D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D009183:Mycotoxins; D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol