title: Protein interaction mapping: a Drosophila case study.
abstract: The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from Drosophila melanogaster, most of them orthologous to human cancer-related and/or signaling proteins, against high-complexity fly cDNA libraries. More than 2300 protein-protein interactions (PPI) were identified, of which 710 are of high confidence. The computation of a reliability score for each protein-protein interaction and the systematic identification of the interacting domain combined with a prediction of structural/functional motifs allow the elaboration of known complexes and the identification of new ones. The full data set can be visualized using a graphical Web interface, the PIMRider (http://pim.hybrigenics.com), and is also accessible in the PSI standard Molecular Interaction data format. Our fly Protein Interaction Map (PIM) is surprisingly different from the one recently proposed by Giot et al. with little overlap between the two data sets. Analysis of the differences in data sets and methods suggests alternative strategies to enhance the accuracy and comprehensiveness of the post-genomic generation of broad-scale protein interaction maps.
authors.affiliation: Hybrigenics, 75014 Paris, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Formstecher
authors.forename: Sandra
authors.initials: S
authors.lastname: Aresta
authors.forename: Vincent
authors.initials: V
authors.lastname: Collura
authors.forename: Alexandre
authors.initials: A
authors.lastname: Hamburger
authors.forename: Alain
authors.initials: A
authors.lastname: Meil
authors.forename: Alexandra
authors.initials: A
authors.lastname: Trehin
authors.forename: Céline
authors.initials: C
authors.lastname: Reverdy
authors.forename: Virginie
authors.initials: V
authors.lastname: Betin
authors.forename: Sophie
authors.initials: S
authors.lastname: Maire
authors.forename: Christine
authors.initials: C
authors.lastname: Brun
authors.forename: Bernard
authors.initials: B
authors.lastname: Jacq
authors.forename: Monique
authors.initials: M
authors.lastname: Arpin
authors.forename: Yohanns
authors.initials: Y
authors.lastname: Bellaiche
authors.forename: Saverio
authors.initials: S
authors.lastname: Bellusci
authors.forename: Philippe
authors.initials: P
authors.lastname: Benaroch
authors.forename: Michel
authors.initials: M
authors.lastname: Bornens
authors.forename: Roland
authors.initials: R
authors.lastname: Chanet
authors.forename: Philippe
authors.initials: P
authors.lastname: Chavrier
authors.forename: Olivier
authors.initials: O
authors.lastname: Delattre
authors.forename: Valérie
authors.initials: V
authors.lastname: Doye
authors.forename: Richard
authors.initials: R
authors.lastname: Fehon
authors.forename: Gérard
authors.initials: G
authors.lastname: Faye
authors.forename: Thierry
authors.initials: T
authors.lastname: Galli
authors.forename: Jean-Antoine
authors.initials: JA
authors.lastname: Girault
authors.forename: Bruno
authors.initials: B
authors.lastname: Goud
authors.forename: Jean
authors.initials: J
authors.lastname: de Gunzburg
authors.forename: Ludger
authors.initials: L
authors.lastname: Johannes
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Junier
authors.forename: Vincent
authors.initials: V
authors.lastname: Mirouse
authors.forename: Ashim
authors.initials: A
authors.lastname: Mukherjee
authors.forename: Dora
authors.initials: D
authors.lastname: Papadopoulo
authors.forename: Franck
authors.initials: F
authors.lastname: Perez
authors.forename: Anne
authors.initials: A
authors.lastname: Plessis
authors.forename: Carine
authors.initials: C
authors.lastname: Rossé
authors.forename: Simon
authors.initials: S
authors.lastname: Saule
authors.forename: Dominique
authors.initials: D
authors.lastname: Stoppa-Lyonnet
authors.forename: Alain
authors.initials: A
authors.lastname: Vincent
authors.forename: Michael
authors.initials: M
authors.lastname: White
authors.forename: Pierre
authors.initials: P
authors.lastname: Legrain
authors.forename: Jérôme
authors.initials: J
authors.lastname: Wojcik
authors.forename: Jacques
authors.initials: J
authors.lastname: Camonis
authors.forename: Laurent
authors.initials: L
authors.lastname: Daviet
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D015723:Gene Library; D017344:Genes, Insect; D011905:Genes, ras; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013045:Species Specificity; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins