title: Inflammatory cytokines reaction elicited by root-end filling materials.
abstract: Cytokines are inflammation in the list of tissue reactions that cytokines control of cell and tissue growth, development, and differentiation. Root-end filling materials often contact with existing periapical tissue, and they need to be biocompatible with remnant periapical tissue. The aim of this study was to focus the effects of the root end filling materials on bone cell viability and expression of inflammatory cytokines and their role in maintaining health and stability of the restored dental tissues. Calcium hydroxide-based (Life), zinc oxide eugenol-based (Super EBA), and mineral trioxide aggregate-based (MTA) root-end filling materials were used to investigate their effect on a human osteosarcoma cell line (U2OS). The cell attachment assay was observed microscopically, and the expression of interleukin-2, -4, and -10 were quantified by enzyme-linked immunosorbent assay. Any resultant difference between the root-end filling material was analyzed statistically by one-way analysis of variance. The results showed that the best cell attachment to root-end filling material occurred with MTA. The IL-4 (0.824 +/- 0.396) and IL-10 (2.06 +/- 1.24) levels were greater for the MTA group, whereas IL-2 expression for the three kinds of root-end filling materials was similar. All materials were able to induce expression of inflammatory cytokines from cultured bone cells.
authors.affiliation: Institute of Medicine, Chung Shan Medical University and Department of Dentistry, Chung Shan Medical Hospital, Taichung, Taiwan.
authors.forename: Tsui Hsien
authors.initials: TH
authors.lastname: Huang
authors.forename: Chi Chiang
authors.initials: CC
authors.lastname: Yang
authors.forename: Sinn Jyh
authors.initials: SJ
authors.lastname: Ding
authors.forename: Ming
authors.initials: M
authors.lastname: Yeng
authors.forename: Chia Tze
authors.initials: CT
authors.lastname: Kao
authors.forename: Ming Yung
authors.initials: MY
authors.lastname: Chou
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival; D016207:Cytokines / Q000737:chemistry* / Q000378:metabolism; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology; D017220:Dentin-Bonding Agents / Q000494:pharmacology; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007249:Inflammation; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D012516:Osteosarcoma / Q000188:drug therapy; D010087:Oxides / Q000494:pharmacology; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D016207:Cytokines; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D007376:Interleukin-2; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D016753:Interleukin-10; D015847:Interleukin-4; C084074:Super EBA; D002126:Calcium Hydroxide