A microarray platform for parallel detection of five transgenic events in foods: a combined polymerase chain reaction-ligation detection reaction-universal array method.
We recently developed a multiplex polymerase chain reaction (PCR) system for the simultaneous detection of four transgenic maize (MON810, Bt176, Bt11, and GA21), one transgenic soybean (Roundup Ready), and two control genes (lectin and zein). Because PCR can lead to ambiguous interpretations due to low specificity, we have developed the ligation detection reaction (LDR) combined with a universal array as a molecular tool to confirm results of PCR analysis. Here, we describe the PCR-LDR-universal array procedure and demonstrate its specificity in revealing the presence of transgenic DNA in experimental samples, raw materials, and commercial foodstuffs.
Bordoni
Roberta
R
Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, I-20090 Segrate (MI), Italy.
Germini
Andrea
A
Mezzelani
Alessandra
A
Marchelli
Rosangela
R
De Bellis
Gianluca
G
D001483:Base Sequence; D018744:DNA, Plant / Q000032:analysis*; D005504:Food Analysis; D020411:Oligonucleotide Array Sequence Analysis; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013025:Glycine max / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D018744:DNA, Plant