title: Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo.
abstract: Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.
authors.affiliation: Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan. y-hamada@sahs.med.osaka-u.ac.jp
authors.forename: Yoshinosuke
authors.initials: Y
authors.lastname: Hamada
authors.forename: Kanako
authors.initials: K
authors.lastname: Yuki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okazaki
authors.forename: Wataru
authors.initials: W
authors.lastname: Fujitani
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Michiyo Kobashi
authors.initials: MK
authors.lastname: Hashida
authors.forename: Kentaro
authors.initials: K
authors.lastname: Harutsugu
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nokihara
authors.forename: Michiharu
authors.initials: M
authors.lastname: Daito
authors.forename: Nariaki
authors.initials: N
authors.lastname: Matsuura
authors.forename: Junzo
authors.initials: J
authors.lastname: Takahashi
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D043925:Angiogenesis Inducing Agents / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000737:chemistry / Q000494:pharmacology* / Q000502:physiology; D014443:Tyrosine / Q000502:physiology
chemical_list: D043925:Angiogenesis Inducing Agents; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D014443:Tyrosine