HMBC-like experiment based on longitudinal csa/dipolar cross-correlation.
A gradient-based sequence is proposed for efficiently filtering out all quantities except the longitudinal two-spin order, created by csa/dipolar cross-correlation rates. The dipolar interaction is between a proton and a heteronucleus, the csa being generally the one of the heteronucleus. Proton detection is carried out to benefit from maximum sensitivity. The resulting two-dimensional spectrum has the same aspect as an HMBC spectrum, implying the existence of a J coupling between the two considered nuclei, but here cross-peaks indicate the strength of the relevant csa/dipolar cross-correlation rate. The method is especially interesting in the case of medium-sized molecules where a given heteronucleus is subjected to several csa/dipolar cross-correlation effects, along with the corresponding J couplings.
Bouguet-Bonnet
Sabine
S
Laboratoire de Méthodologie RMN, Université Henri Poincaré Faculté des Sciences, BP 239, 54506 Vandoeuvre-lès-Nancy Cedex, France.
Leclerc
Sébastien
S
Mutzenhardt
Pierre
P
Canet
Daniel
D
D002247:Carbon Isotopes; D046911:Macromolecular Substances; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D012680:Sensitivity and Specificity
D002247:Carbon Isotopes; D046911:Macromolecular Substances; D011522:Protons