[Knockdown of STAT3 expression using siRNA inhibits the growth of prostate cancer cell lines].
OBJECTIVE
To study the effects of pSilencer 1.0-U6-siRNA-STAT3 on the growth of PC3 and LNCaP cells.


METHODS
Three pairs of DNA template coding siRNA were synthesized against STAT3 to reconstruct pSilencer 1.0-U6-STAT3-siRNA, which was transfected into PC3 and LNCaP cells. The STAT3 expression in PC3 cells and LNCaP were transfected with pSilencer 1.0-U6-siRNA-STAT3, and it was detected by Western blot and Northern blot. MTT and FCM were used to observe the growth-inhibiting ratio and apoptosis in PC3 cells.


RESULTS
Western blot and Northern blot analyses demonstrated that pSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit the expression of STAT3 in PC3 and LNCaP cells; MIT and FCM results showed that it could suppress the growth of PC3 cells and LNCaP and induce apoptosis of PC3 cells in vitro.


CONCLUSIONS
PSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of PC3 and LNCaP cells and induce the apoptosis of PC3 cells.
Gao
Li-fang
LF
Department of Pathophysiology, School of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
Xu
De-qi
DQ
Shao
Yue-ting
YT
Zhao
Dan
D
Zhao
Xue-jian
XJ
D017209:Apoptosis; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor