title: A thermodynamic study of mesophilic, thermophilic, and hyperthermophilic L-arabinose isomerases: the effects of divalent metal ions on protein stability at elevated temperatures.
abstract: To gain insight into the structural stability of homologous homo-tetrameric l-arabinose isomerases (AI), we have examined the isothermal guanidine hydrochloride (GdnHCl)-induced unfolding of AIs from mesophilic Bacillus halodurans (BHAI), thermophilic Geobacillus stearothermophilus (GSAI), and hyperthermophilic Thermotoga maritima (TMAI) using circular dichroism spectroscopy. The GdnHCl-induced unfolding of the AIs can be well described by a two-state reaction between native tetramers and unfolded monomers, which directly confirms the validity of the linear extrapolation method to obtain the intrinsic stabilities of these proteins. The resulting unfolding free energy (DeltaGU) values of the AIs as a function of temperature were fit to the Gibbs-Helmholtz equation to determine their thermodynamic parameters based on a two-state mechanism. Compared with the stability curves of BHAI in the presence and absence of Mn2+, those of holo GSAI and TMAI were more broadened than those of the apo enzymes at all temperatures, indicating increased melting temperatures (Tm) due to decreased heat capacity (DeltaGp). Moreover, the extent of difference in DeltaCp between the apo and holo thermophilic AIs is larger than that of BHAI. From these studies, we suggest that the metal dependence of the thermophilic AIs, resulting in the reduced DeltaCp, may play a significant role in structural stability compared to their mesophilic analogues, and that the extent of metal dependence of AI stability seems to be highly correlated to oligomerization.
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Lee
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Hong
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Choe
authors.forename: Sang-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Seong-Bo
authors.initials: SB
authors.lastname: Kim
authors.forename: Han-Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Oh
authors.forename: Hae-Hun
authors.initials: HH
authors.lastname: Shin
authors.forename: Yu-Ryang
authors.initials: YR
authors.lastname: Pyun
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry / Q000378:metabolism*; D001406:Bacillaceae / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D002413:Cations, Divalent / Q000494:pharmacology*; D002942:Circular Dichroism; D004795:Enzyme Stability / Q000187:drug effects; D019791:Guanidine / Q000494:pharmacology; D006358:Hot Temperature; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D013816:Thermodynamics; D020124:Thermotoga maritima / Q000201:enzymology; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase; D015032:Zinc; D019791:Guanidine