title: Dietary unsaturated long-chain Fatty acids modify D-glucose absorption in weaning rats.
abstract: OBJECTIVE
  The authors evaluated the effects of dietary long-chain polyunsaturated fatty acids on D-glucose absorption in weaning rats.
  
  
  METHODS
  Pups were born from control mothers fed a diet containing (per kg of total fatty acids) 280 g of saturated fatty acids, 496 g of monounsaturated fatty acids and 222 g of polyunsaturated fatty acids or from mothers fed a diet containing a high proportion of saturated fatty acids (920 g/kg) and a low proportion of unsaturated fatty acids (low-unsaturated fatty acid, 80 g/kg), initiated 2 weeks before mating and continued throughout pregnancy. When pups from low-unsaturated fatty acid mothers were 15 days old, they were subdivided into two groups: one control (low-unsaturated fatty acid-C) and one fed a long-chain polyunsaturated fatty acid supplement rich in arachidonic acid and docosahexaenoic acid (low-unsaturated fatty acid-S) until weaning. At day 21, the kinetics of D-glucose absorption was studied in brush-border membrane vesicles from the jejunoileal segment.
  
  
  RESULTS
  The maximal transport rate (V(max)) of glucose in the low-unsaturated fatty acid-C and low-unsaturated fatty acid-S groups was higher than in control rats: 160 and 130 versus 98 pmol/(mg protein.s), respectively (P < 0.05). Rats fed the low-unsaturated fatty acid diet had a lower diffusion constant (K(d)) than control rats did: 21.6 and 29.2 nL/(mg protein.s), respectively (P < 0.05). However, rats receiving the long-chain polyunsaturated fatty acid supplement and control rats had similar Kd values.
  
  
  CONCLUSIONS
  These results indicate that dietary long-chain polyunsaturated fatty acid supplementation can restore, in part, the kinetic characteristics of intestinal D-glucose absorption in pups from mothers maintained on a low-unsaturated fatty acid diet.
authors.affiliation: Grup de Fisiologia i Nutrició Experimental, Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, E-08028 Barcelona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Vicario
authors.forename: Elvira
authors.initials: E
authors.lastname: Pedragosa
authors.forename: Montserrat
authors.initials: M
authors.lastname: Rivero
authors.forename: Miquel
authors.initials: M
authors.lastname: Moretó
authors.forename: Concepció
authors.initials: C
authors.lastname: Amat
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D019540:Area Under Curve; D001693:Biological Transport, Active / Q000187:drug effects; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000493:pharmacokinetics*; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014886:Weaning
chemical_list: D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D005947:Glucose