title: Developmental expression of FXPRLamide neuropeptides in peptidergic neurosecretory cells of diapause- and nondiapause-destined individuals of the cotton bollworm, Helicoverpa armigera.
abstract: The diapause hormone (DH)-pheromone biosynthesis activating neuropeptide (PBAN) gene encodes five neuropeptides, DH, PBAN, alpha-SGNP, beta-SGNP, and gamma-SGNP (subesophageal ganglion neuropeptide). All share the C-terminal pentapeptide FXPRLamide sequence and are produced in the subesophageal ganglion (SG). Expression of the DH-PBAN gene in the central nervous system of embryonic, larval, pupal, and adult Helicoverpa armigera (Har) was studied using in situ hybridization, whole-mount immunocytochemistry, and competitive ELISA. Both Har-DH-PBAN mRNA and protein are localized in the mandibular, maxillary, and labial cell clusters of the SG and a pair of ventral midline neurons of each thoracic ganglion. The FXPRLamide titers in hemolymph are significantly higher in diapause-destined larvae during the fifth and sixth instar than in similar nondiapause-destined individuals. In contrast, the FXPRLamide titers in diapause-destined pupae are significantly lower than in nondiapause-destined pupae. The results from immunocytochemistry and in situ hybridization are consistent with changes of FXPRLamide titers as measured by ELISA. These data suggest that the expression of DH-PBAN might be correlated with diapause induction at the larval stage of diapause-destined individuals and continuous development at pupal stage of nondiapause-destined individuals. Thus, the DH-PBAN gene may play an important regulatory role in aspects of insect development besides diapause termination and pheromone biosynthesis. The transport pathways of FXPRLamide neuropeptides suggest that humoral route is involved in their regulation of development.
authors.affiliation: Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
authors.forename: Jiu-Song
authors.initials: JS
authors.lastname: Sun
authors.forename: Qi-Rui
authors.initials: QR
authors.lastname: Zhang
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Zhang
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Zhu
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Zhang
authors.forename: Mai-Kun
authors.initials: MK
authors.lastname: Teng
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Niu
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Xu
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D018507:Gene Expression Regulation, Developmental; D006458:Hemolymph; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007814:Larva / Q000254:growth & development; D009036:Moths / Q000235:genetics* / Q000254:growth & development*; D009479:Neuropeptides / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D009479:Neuropeptides; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea