Characterization of (123)I-iodo-alpha-methyltyrosine transport in rat lymphoma cells.
(123)I-Iodo-alpha-methyltyrosine (IMT) transport into lymphomas has not been fully characterized. In rat Nb2-11C and Nb2-Sp lymphoma cell lines, linear uptake of (123)I-IMT occurred rapidly within 5-10 min. Eadie-Hoftee plots of (123)I-IMT uptake gave apparent Km's of 8.34+/-1.17 and 9.64+/-1.05 microM for Nb2-11C and Nb2-Sp cells, respectively, and involved the L and B(0,+) systems. In lymphoma-bearing rats, injected (123)I-IMT accumulated rapidly in the primary tumors but gave a low tumor-to-background ratio of 2:1. (123)I-IMT was transported rapidly into lymphoma cells both in vitro and in vivo, but low target-to-nontarget ratio may not make (123)I-IMT practical for scanning in vivo.
Matte
Gilbert
G
Department of Diagnostic Imaging, QEII Health Sciences Centre, 1278 Tower Road, VG Site, Halifax, Nova Scotia, B3H 2Y9, Canada. gmatte@dal.ca
Sangster
Shirley M
SM
Acker
Matthew
M
Hudgins
Marlene
M
Too
Catherine K L
CK
D000818:Animals; D001693:Biological Transport, Active; D045744:Cell Line, Tumor; D007668:Kidney / Q000000981:diagnostic imaging* / Q000378:metabolism*; D008223:Lymphoma / Q000000981:diagnostic imaging* / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D008781:Methyltyrosines / Q000493:pharmacokinetics*; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D014018:Tissue Distribution
D008781:Methyltyrosines; D019275:Radiopharmaceuticals; C030855:3-iodo-alpha-methyltyrosine