title: Fully automated micro- and nanoscale one- or two-dimensional high-performance liquid chromatography system for liquid chromatography-mass spectrometry compatible with non-volatile salts for ion exchange chromatography.
abstract: A one- or two-dimensional high performance liquid chromatography system for electrospray ionization mass spectrometers has been developed that is optimized for ion exchange and reversed phase separations. A unique and simple valve configuration permits the use of a variety of non-volatile salts; ammonium sulfate was used in an example of strong cation exchange separations. The system was designed and evaluated for both micro- and nanoflow chromatography. The peptide detection limit was approximately 100 fmol for micro- and 20 fmol for nanoflow, demonstrating the concentration and mass sensitivity improvements expected with nanoelectrospray ionization. The 1D/2D-HPLC MS system is fully automated for routine peptide analyses, compatible with direct injection of proteolytic digests, and exhibits chromatographic reproducibility and sensitivity. Software permits operator selection of either a 1D or 2D configuration with corresponding system parameters as required for individual samples. The hardware elements and resulting performance are described in this paper.
authors.affiliation: Laboratory of Neurotoxicology, National Institute of Mental Health, National Institute of Health, Bethesda, MD 20892-1262, USA.
authors.forename: Junichi
authors.initials: J
authors.lastname: Masuda
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Maynard
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nishimura
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Ueda
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Kowalak
authors.forename: Sanford P
authors.initials: SP
authors.lastname: Markey
mesh_terms: D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D036103:Nanotechnology; D010455:Peptides / Q000032:analysis; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010455:Peptides; D012492:Salts