Identification and characterization of human ZNF18 gene in silico.
Zinc finger protein genes are regulatory transcription factors that interact directly with DNA and/or RNA. Here, we identified the ZNF18 gene by using bioinformatics. Human ZNF18 gene, consisting of 9 exons, was located within human genome sequences RP11-1096G20 (AC005410.3). Complete coding sequence of human ZNF18 cDNA was determined by FLJ35337 cDNA (AK092656), which was identified by using partial coding sequence of human ZNF18 cDNA (X52342) as a query. Human, mouse, and rat ZNF18 showed 77-92% total-amino acid identity. N-terminal leucine rich region, krueppel-associated box, and C-terminal three C2H2 type zinc finger motifs were identified within ZNF18 protein. This is the first report on identification and characterization of the ZNF18 gene.
Yoshida
Kenichi
K
Department of Life Sciences, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan. yoshida@isc.meiji.ac.jp
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D016415:Sequence Alignment; D016335:Zinc Fingers
D018076:DNA, Complementary; D051741:Kruppel-Like Transcription Factors; D016601:RNA-Binding Proteins; C500914:ZNF18 protein, human