Synthesis of homogenous site-selectively glycosylated proteins.
Apparently homogenous glycoproteins can be synthesised in good yield by a combination of site directed mutagenesis, a highly flexible but selective chemical derivatisation and efficient purification through the use of glycosyl thiosulfonates such as 2-((biotinoyl)-amino)-ethyl methanethiosulfonate.
Swanwick
Richard S
RS
School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Daines
Alison M
AM
Flitsch
Sabine L
SL
Allemann
Rudolf K
RK
D002241:Carbohydrates / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology; D005020:Ethyl Methanesulfonate / Q000031:analogs & derivatives / Q000737:chemistry; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000235:genetics / Q000302:isolation & purification; D006031:Glycosylation; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry
D002241:Carbohydrates; D006023:Glycoproteins; D010446:Peptide Fragments; C093171:methanethiosulfonate ethylammonium; D005020:Ethyl Methanesulfonate; D013762:Tetrahydrofolate Dehydrogenase; D003545:Cysteine