Effects of isoflurane on measurements of delayed lumininescence in Acetabularia acetabulum.
The volatile halogenated methyl ethyl ether, isoflurane, used as an anaesthetic, inhibits actin-based dynamics directly or indirectly in animal cells. In plant cells, most intracellular movements are related to actin pathways. We have used isoflurane in a unicellular alga, Acetabularia acetabulum, to test the dynamics of choloroplast organization. By measuring the delayed luminescence, we found that isoflurane worked efficiently in the unicellular organism and showed dose- and time-course-dependent actin-inhibition patterns. When A. acetabulum was treated with saturated solutions of isoflurane in artificial seawater (defined as 100% isoflurane) for 3 or 6 min, the delayed luminescence (DL) was decreased and was never recovered. In contrast, if treated with 75% diluted isoflurane, the DL was firstly inhibited and then recovered several hours later, and if treated with 50% diluted isoflurane, the change of DL was small. Our work proved that isoflurane can affect actin-related pathways in both animals and plants.
Chen
Wen Li
WL
College of Life Science, South China Normal University, Guangzhou 510631, People's Republic of China. chenwl@scnu.edu.cn
Van Wijk
Roeland
R
Xing
Da
D
D000076:Acetabularia / Q000737:chemistry*; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D007530:Isoflurane / Q000494:pharmacology*; D008163:Luminescent Measurements; D013997:Time Factors
D018685:Anesthetics, Inhalation; D007530:Isoflurane