title: Overexpression of heparin-binding growth-associated molecule in malignant glioma cells.
abstract: The highly invasive and angiogenic characteristics of malignant gliomas depend on the production of growth factors and angiogenic factors. Heparin-binding growth-associated molecule (HB-GAM) is a secreted growth factor that is mitogenic for endothelial cells. To examine the expression profile of HB-GAM in malignant glioma cells, messenger ribonucleic acid (mRNA) expression was analyzed in 10 malignant glioma cell lines, two glioblastoma tissue specimens, and two normal brain tissue specimens by the reverse transcription-polymerase chain reaction. HB-GAM mRNA was expressed in all specimens including normal brain tissue specimens. Western blot analysis revealed that HB-GAM protein contents in glioma cell lines and glioblastoma tissues were 1.8 to 6.3 times higher than those in normal brain tissues. The effect of neutralizing anti-platelet-derived growth factor (PDGF) antibody was also examined on the production of HB-GAM in malignant glioma cells, since malignant glioma cells secrete PDGF that upregulates HB-GAM expression. Treatment of U251 and T98G glioblastoma cells with the anti-PDGF antibody did not affect the HB-GAM production. These results suggest that HB-GAM is overexpressed in malignant glioma cells and is involved in tumor growth.
authors.affiliation: Department of Neurosurgery, University of Yamanashi, Faculty of Medicine, Yamanashi, Japan.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Tadashi
authors.initials: T
authors.lastname: Mabuchi
authors.forename: Eiji
authors.initials: E
authors.lastname: Satoh
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Maeda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nukui
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Naganuma
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D016207:Cytokines; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; C066845:pleiotrophin