title: Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus.
abstract: To identify the function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome (SARS) coronavirus (CoV), we analyzed the protein-protein interaction among HAb18G/CD147, cyclophilin A (CyPA), and SARS-CoV structural proteins by coimmunoprecipitation and surface plasmon resonance analysis. Although none of the SARS-CoV proteins was found to be directly bound to HAb18G/CD147, the nucleocapsid (N) protein of SARS-CoV was bound to CyPA, which interacted with HAb18G/CD147. Further research showed that HAb18G/CD147, a transmembrane molecule, was highly expressed on 293 cells and that CyPA was integrated with SARS-CoV. HAb18G/CD147-antagonistic peptide (AP)-9, an AP of HAb18G/CD147, had a high rate of binding to 293 cells and an inhibitory effect on SARS-CoV. These results show that HAb18G/CD147, mediated by CyPA bound to SARS-CoV N protein, plays a functional role in facilitating invasion of host cells by SARS-CoV. Our findings provide some evidence for the cytologic mechanism of invasion by SARS-CoV and provide a molecular basis for screening anti-SARS drugs.
authors.affiliation: Department of Cell Biology, the Fourth Military Medical University, Xi'an, China.
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Mi
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Jiyun
authors.initials: J
authors.lastname: Yu
authors.forename: Xianhui
authors.initials: X
authors.lastname: Wang
authors.forename: Jianli
authors.initials: J
authors.lastname: Jiang
authors.forename: Jinliang
authors.initials: J
authors.lastname: Xing
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
authors.forename: Airong
authors.initials: A
authors.lastname: Qian
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Peter X
authors.initials: PX
authors.lastname: Shaw
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.forename: Shumin
authors.initials: S
authors.lastname: Duan
authors.forename: Jin
authors.initials: J
authors.lastname: Ding
authors.forename: Chunmei
authors.initials: C
authors.lastname: Fan
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Yu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Feng
authors.forename: Biehu
authors.initials: B
authors.lastname: Li
authors.forename: Xiying
authors.initials: X
authors.lastname: Yao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xue
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
mesh_terms: D015703:Antigens, CD / Q000502:physiology*; D051926:Basigin; D002460:Cell Line; D000086462:Coronavirus Nucleocapsid Proteins; D021984:Cyclophilin A / Q000502:physiology; D003588:Cytopathogenic Effect, Viral / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D019590:Nucleocapsid Proteins / Q000502:physiology; D011485:Protein Binding; D045473:Severe acute respiratory syndrome-related coronavirus / Q000472:pathogenicity*; D015678:Viral Structural Proteins / Q000502:physiology*
chemical_list: D015703:Antigens, CD; C497886:BSG protein, human; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D015678:Viral Structural Proteins; D051926:Basigin; D021984:Cyclophilin A