Antibody-based proteomics for human tissue profiling.
Here, we describe the use of antibody-based proteomics involving the generation of protein-specific antibodies to functionally explore the human proteome. The antibodies can be used for analysis of corresponding proteins in a wide range of assay platforms, including i) immunohistochemistry for detailed tissue profiling, ii) specific affinity reagents for various functional protein assays, and iii) capture ("pull-down") reagents for purification of specific proteins and their associated complexes for structural and biochemical analyses. In this review, the use of antibodies for such analysis will be discussed with focus on the possibility to create a descriptive and comprehensive protein atlas for tissue distribution and subcellular localization of human proteins in both normal and disease tissues.
Uhlen
Mathias
M
Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE-10691 Stockholm, Sweden. mathias.uhlen@biotech.kth.se
Ponten
Fredrik
F
D000906:Antibodies / Q000276:immunology*; D006801:Humans; D040081:Protein Array Analysis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D040901:Proteomics; D014018:Tissue Distribution
D000906:Antibodies; D011506:Proteins; D020543:Proteome