title: Detection of nonpigmented endometriotic lesions with 5-aminolevulinic acid-induced fluorescence.
abstract: OBJECTIVE
  To evaluate the feasibility of fluorescence diagnosis of nonpigmented (red and white) endometriotic lesions with 20 mg/kg of 5-aminolevulinc acid (5-ALA) 5-7 and 10-14 hours before surgery.
  
  
  METHODS
  Prospective analysis (Canadian Task Force classification II-2).
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Twenty-four consecutive patients with suspected endometriosis undergoing laparoscopy.
  
  
  METHODS
  Laparoscopic surgery under white light illumination and fluorescence diagnosis.
  
  
  RESULTS
  The total number of endometriotic lesions detected under white light illumination was compared with the number of lesions detected with fluorescence diagnosis. Fluorescence diagnosis yielded an overall improvement of 35% in the detection of nonpigmented endometriotic lesions compared with white light illumination. Sensitivity (91%) and specificity (79%) were similar 5-7 and 10-14 hours before surgery.
  
  
  CONCLUSIONS
  The dosage of 20 mg/kg body weight of 5-ALA is feasible for fluorescence diagnosis of nonpigmented endometriosis. Sensitivity of fluorescence diagnosis with 20 mg/kg is similar to that achieved with a 30-mg/kg dose. Sensitivity does not change within the application period 5-7 and 10-14 hours before surgery.
authors.affiliation: Department of Obstetrics and Gynecology, University of Münster, Albert Schweitzer Strasse 33, D-48149 Münster, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Buchweitz
authors.forename: Pia
authors.initials: P
authors.lastname: Wülfing
authors.forename: Anette
authors.initials: A
authors.lastname: Staebler
authors.forename: Ludwig
authors.initials: L
authors.lastname: Kiesel
mesh_terms: D000328:Adult; D000622:Aminolevulinic Acid; D004715:Endometriosis / Q000175:diagnosis*; D005260:Female; D005453:Fluorescence; D006801:Humans; D010535:Laparoscopy / Q000379:methods; D017319:Photosensitizing Agents; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid