Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa.
Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with the sizes of compact callus aggregates (CCA) and cellular viability. In the red cell line, the sizes of CCA were predominantly of 2-4 mm diameter and accounted for 64% biomass. This line had a sustained cell viability over 10 successive sub-cultures.
Fu
Chun-xiang
CX
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, P.R. China, 100093.
Zhao
De-xiu
DX
Huang
Yan
Y
Ma
Feng-shan
FS
D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D048429:Cell Size; D002470:Cell Survival; D047309:Flavones / Q000096:biosynthesis*; D005419:Flavonoids / Q000096:biosynthesis*; D030441:Saussurea / Q000166:cytology* / Q000378:metabolism*
D047309:Flavones; D005419:Flavonoids; C477508:jaceosidin; C055957:hispidulin