Murine gammaherpesvirus 68 open reading frame 11 encodes a nonessential virion component.
Open reading frame 11 (ORF11) is a conserved gammaherpesvirus gene that remains undefined. We identified the product of murine gammaherpesvirus 68 (MHV-68) ORF11, p43, as a virion component with a predominantly perinuclear distribution in infected cells. MHV-68 lacking p43 grew normally in vitro but showed reduced lytic replication in vivo and a delay in seeding to the spleen. Subsequent latency amplification was normal. Thus, MHV-68 ORF11 encoded a virion component that was important for in vivo lytic replication but dispensable for the establishment of latency.
Boname
Jessica M
JM
Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Rd., Cambridge CB2 1QP, United Kingdom.
May
Janet S
JS
Stevenson
Philip G
PG
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000209:etiology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016366:Open Reading Frames; D018144:Rhadinovirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D014412:Tumor Virus Infections / Q000209:etiology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D004279:DNA, Viral; D014764:Viral Proteins