[Asepsis sowing and tissue culture of Bletilla striata].
The asepsis sowing and tissue culture of Bletilla striata were studied. The results indicated that the embyro culture had highest sprouting percent and plantlets percent when their embryos were mature. The optimal medium for the embryo culture was 1/2 MS. Adding 10% coconut juice can promote embyros sprouting and plantlets formation, 1% active carbon can improve plantlets growing. The best medium for the top of plantlet on culture and multiplication was 1/2 MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L. The best medium of rooting was 1/2 MS + NAA 0.5 mg/L, and 10% banana juice can improve rooting of plantlets.
Zeng
Songjun
S
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510520.
Huang
Xiangli
X
Chen
Zhilin
Z
Chen
Jiantong
J
Duan
Jun
J
D003470:Culture Media; D029595:Orchidaceae / Q000737:chemistry / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media; D010937:Plant Growth Regulators