title: SOS-independent induction of dinB transcription by beta-lactam-mediated inhibition of cell wall synthesis in Escherichia coli.
abstract: Transcription of the dinB gene, encoding DNA polymerase IV, is induced by the inhibition of cell wall synthesis at different levels. Using the beta-lactam antibiotic ceftazidime, a PBP3 inhibitor, as a model, we have shown that this induction is independent of the LexA/RecA regulatory system. Induction of dinB transcription mediated by ceftazidime produces an increase in the reversion of a +1 Lac frameshift mutation.
authors.affiliation: Departamento de Biotecnología Microbiana, Centro Nacional de Biotechnología, Universidad Autónoma de Madrid, Spain.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Pérez-Capilla
authors.forename: María-Rosario
authors.initials: MR
authors.lastname: Baquero
authors.forename: José-María
authors.initials: JM
authors.lastname: Gómez-Gómez
authors.forename: Alina
authors.initials: A
authors.lastname: Ionel
authors.forename: Soledad
authors.initials: S
authors.lastname: Martín
authors.forename: Jesús
authors.initials: J
authors.lastname: Blázquez
mesh_terms: D000251:Adenosine Triphosphatases / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology; D002442:Ceftazidime / Q000494:pharmacology*; D002473:Cell Wall / Q000378:metabolism*; D004265:DNA Helicases / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D016368:Frameshift Mutation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D013014:SOS Response, Genetics; D012697:Serine Endopeptidases / Q000502:physiology; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics; D000099160:LexA Repressor Protein
chemical_list: D000251:Adenosine Triphosphatases; D001426:Bacterial Proteins; D002442:Ceftazidime; D004265:DNA Helicases; D004791:Enzyme Inhibitors; D029968:Escherichia coli Proteins; D012697:Serine Endopeptidases; D001616:beta-Galactosidase; D000099160:LexA Repressor Protein; C094399:DinB protein, E coli