title: [Surfactant protein A gene polymorphisms in chronic obstructive pulmonary disease].
abstract: OBJECTIVE
  To investigate whether polymorphism in gene for surfactant protein A(SPA) has any bearing on individual susceptibility to the development of chronic obstructive pulmonary disease(COPD).
  
  
  METHODS
  The genotypes of 88 patients with COPD and 87 healthy smoking subjects as controls were tested with polymerase chain reaction followed by restriction fragment length polymorphism analysis for SPA gene.
  
  
  RESULTS
  In COPD group, the frequencies of +186 locus genotypes AA, AG and GG were 86.4%, 12.5% and 1.1%i respectively and in the control group, these were 66.7%, 27.6% and 5.7%; the frequencies of polymorphic genotypes or alleles showed statistically significant difference between the COPD group and the control group(P<0.05). The frequencies of polymorphic genotypes at +655 locus and +667 loci showed no significant difference between the COPD group and the control group in(P>0.05).
  
  
  CONCLUSIONS
  Genetic polymorphism in SPA is associated with the development of COPD in the Chinese Hans.
authors.affiliation: Department of Respiratory Medicine, Tongji Hospital of Tongji Medical College, Huazhong Science and Technology University, Wuhan, Huibei, 430030 PR China.
authors.forename: Jun-gang
authors.initials: JG
authors.lastname: Xie
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Xu
authors.forename: Zhen-xiang
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Wang
authors.initials: W
authors.lastname: Ni
authors.forename: Shi-xin
authors.initials: SX
authors.lastname: Chen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics*; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics*
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A