title: Covert signal disruption: anti-ecdysteroidal activity of bisphenol A involves cross talk between signaling pathways.
abstract: Bisphenol A is a key industrial chemical used in the manufacture of polycarbonate plastics and other products. Several recent reports ascribe toxicological properties to this compound that have been attributed to the disruption of endocrine-related processes. In the present study, the toxicity of bisphenol A was definitively characterized in the water flea (Daphnia magna) in an effort to discern whether this compound may elicit endocrine toxicity in an invertebrate species and to establish the mechanism by which this toxicity is elicited. The ability of bisphenol A to interfere with two ecdysteroid-dependent physiological processes--molting and embryonic development--was evaluated. Bisphenol A elicited antiecdysteroidal activity as indicated by its prolongation of the intermolt period and interference with embryonic development. This apparent antiecdysteroidal activity was not due to reduced availability of endogenous ecdysteroid nor due to ecdysteroid-receptor antagonism. The ability of bisphenol A to elicit antiecdysteroidal activity by functioning as a juvenoid hormone was next evaluated. Bisphenol A, alone, did not elicit juvenoid activity. However, bisphenol A did enhance the activity of the crustacean juvenoid hormone methyl farnesoate. A definitive assessment of the effects of bisphenol A on the reproductive capacity of daphnids revealed a concentration-response relationship that extended at least one order of magnitude below exposure levels that were overtly toxic to the maternal organisms. These results demonstrate that bisphenol A is chronically toxic to daphnids, probably through its ability to interfere with ecdysteroid/juvenoid regulated processes. However, effects are elicited at levels that are not likely to pose environmental concern.
authors.affiliation: Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, North Carolina 27695-7633, USA.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Mu
authors.forename: Cynthia V
authors.initials: CV
authors.lastname: Rider
authors.forename: Gap Soo
authors.initials: GS
authors.lastname: Hwang
authors.forename: Heather
authors.initials: H
authors.lastname: Hoy
authors.forename: Gerald A
authors.initials: GA
authors.lastname: LeBlanc
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D003621:Daphnia / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D026461:Ecdysteroids / Q000037:antagonists & inhibitors*; D004441:Ecdysterone / Q000494:pharmacology; D007605:Juvenile Hormones / Q000502:physiology; D018978:Molting / Q000187:drug effects; D010636:Phenols / Q000037:antagonists & inhibitors / Q000633:toxicity*; D012733:Sex Differentiation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D013995:Time; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D026461:Ecdysteroids; D004441:Ecdysterone; D007605:Juvenile Hormones; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds