Ontogeny of pituitary thyrotrophs and regulation by endogenous thyroid hormone feedback in the chick embryo.
Increased thyroid hormone production is essential for hatching of the chick and for the increased metabolism necessary for posthatch endothermic life. However, little is known about the ontogeny and distribution of pituitary thyrotrophs during this period or whether pituitary thyroid-stimulating hormone (TSH) production is regulated by endogenous thyroid hormones during chick embryonic development. This study assessed the abundance and location of pituitary thyrotrophs and the regulation of TSH(beta) peptide and mRNA levels by endogenous thyroid hormones prior to hatching. TSH(beta)-containing cells were first detected on embryonic day (e) 11, and the thyrotroph population increased to maximum levels on e17 and e19 and then decreased prior to hatching (d1). Thyrotroph distribution within the cephalic lobe of the anterior pituitary was determined on e19 by whole-mount immunocytochemistry for TSH(beta) peptide and by whole-mount in situ hybridization for TSH(beta) mRNA. Thyrotroph distribution within the cephalic lobe was heterogeneous among embryos, but most commonly extended from the ventral medial region to the dorsal lateral regions, along the boundary of the cephalic and caudal lobes. Inhibition of endogenous thyroid hormone production with methimazole (MMI) decreased plasma thyroxine (T4) levels and increased pituitary TSH(beta) mRNA levels on e19 and d1. However, control pituitaries contained significantly more TSH(beta) peptide than MMI-treated pituitaries on e17 and e19, suggesting higher TSH secretion into the blood in MMI-treated groups. We conclude that thyrotroph abundance and TSH production increase prior to hatching, that thyrotrophs are localized heterogeneously within the cephalic lobe of the anterior pituitary at that time, and that TSH gene expression and secretion are under negative feedback regulation from thyroid hormones during this critical period of development.
Muchow
Michael
M
Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA.
Bossis
Ioannis
I
Porter
Tom E
TE
D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002642:Chick Embryo / Q000166:cytology / Q000502:physiology*; D025461:Feedback, Physiological; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D008713:Methimazole / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D037322:Thyrotropin, beta Subunit / Q000032:analysis / Q000235:genetics; D013974:Thyroxine / Q000097:blood / Q000378:metabolism*
D013956:Antithyroid Agents; D012333:RNA, Messenger; D037322:Thyrotropin, beta Subunit; D008713:Methimazole; D013974:Thyroxine