Evidence for interspecific-recombination for three monopartite begomoviral genomes associated with the tomato leaf curl disease from central Sudan.
Two distinct viral genotypes were identified in the same tomato plant collected from Gezira, Sudan and are provisionally designated Tomato leaf curl Sudan virus (ToLCSDV-Gez) and Tomato yellow leaf curl virus-Sudan (TYLCV-SD). A third genotype was identified in tomato samples collected in Shambat, Sudan (ToLCSDV-Sha). The ToLCSDV-Gez and ToLCSDV-Sha isolates were approximately 90% identical, TYLCV-SD from Gezira shared approximately 93% identity with TYLCV-Mld. Recombination analyses identified two fragments in the ToLCSDV-Gez and TYLCV-SD genomes, providing evidence that these two genomes had undergone intermolecular recombination. A half unit size (737 nt) single-stranded satellite DNA was associated with ToLCSDV-Gez and TYLCV-SD.
Idris
A M
AM
Department of Plant Sciences, The University of Arizona, Tucson, Arizona 85721, USA.
Brown
J K
JK
D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013397:Sudan
D004276:DNA, Satellite; D004279:DNA, Viral