[The responses of AtJ2 and AtJ3 gene expression to environmental stresses in Arabidopsis].
By using Northern analysis of the 3''terminal noncoding region of AtJ2 and AtJ3 genes obtained by PCR, it was found that AtJ2 and AtJ3 genes were constitutively expressed in Arabidopsis roots, stems, leaves, flower buds, flowers and silique. They were expressed during the whole growth phase, but the mRNA level decreased slowly with the senescing of plants. Heat shock at 37 degrees C and cold stress at 2 degrees C induced an increase in mRNA level of AtJ2 and AtJ3, but the temporal characteristics of expression of AtJ2 and AtJ3 genes demonstrated that the response of the two genes to cold stress was much slower than that to heat shock. The expression of AtJ2 and AtJ3 genes was up-regulated slightly by water stress. Salt stress had no effect on the expression of AtJ2 and AtJ3 in the experiments. These results indicate that the expression of AtJ2 and AtJ3 genes is involved in response to many environmental stresses except salt stress.
Li
Guo-Liang
GL
Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China.
Li
Bing
B
Liu
Hong-Tao
HT
Zhou
Ren-Gang
RG
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D015152:Blotting, Northern; D003080:Cold Temperature; D055864:Droughts; D035264:Flowers / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
C494633:ATJ2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050956:HSP40 Heat-Shock Proteins; D010937:Plant Growth Regulators; D012965:Sodium Chloride