lux-marked Pseudomonas aeruginosa lipopolysaccharide production in the presence of rhamnolipid.
Rhamnolipid produced by Pseudomonas aeruginosa during the late logarithmic growth phase has been reported to be an effective biosurfactant. In the presence of rhamnolipid, P. aeruginosa had a higher specific lipopolysaccharide production, which was attributed to the increase of carbon to nitrogen ratios owing to the increase of the solubility of hydrophobic hydrocarbons, favoring the synthesis of lipopolysaccharides. P. aeruginosa also had a higher ratio of lipopolysaccharides in the growth medium to those on cell surfaces than in the absence of rhamnolipid because the presence of rhamnolipid stimulated the release of lipopolysaccharides from P. aeruginosa cell outer membranes. The release of lipopolysaccharides from cell surfaces made P. aeruginosa exhibit a more hydrophobic surface, enhancing the accumulation of hydrophobic hydrocarbons on P. aeruginosa cell surfaces and consequently resulting in a higher growth rate.
Chen
Gang
G
Crop and Soil Sciences, Washington State University, Pullman, WA 99164, USA. chen9046@wsu.edu
Zhu
Honglong
H
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics; D015415:Biomarkers / Q000737:chemistry; D006017:Glycolipids / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D015415:Biomarkers; D006017:Glycolipids; D008070:Lipopolysaccharides; D008164:Luminescent Proteins; C418382:rhamnolipid