[Determination of glycyrrhizinic acid in biotransformation system by reversed-phase high performance liquid chromatography].
A method for determining glycyrrhizinic acid in the biotransformation system by reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The HPLC conditions were as follows: Hypersil C18 column (4.6 mm i.d. x 250 mm, 5 microm) with a mixture of methanol-water-acetic acid (70:30:1, v/v) as the mobile phase; flow rate at 1.0 mL/min; and UV detection at 254 nm. The linear range of glycyrrhizinic acid was 0.2-20 microg. The recoveries were 98%-103% with relative standard deviations between 0.16% and 1.58% (n = 3). The method is simple, rapid and accurate for determining glycyrrhizinic acid.
Li
Hui
H
College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
Lu
Dingqiang
D
Liu
Weimin
W
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D019695:Glycyrrhizic Acid / Q000378:metabolism*; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D019695:Glycyrrhizic Acid