[Organelle redistribution during PK cell spreading in normal conditions and in the presence of sodium azide].
Cell detachment from solid substratum results in a changed arrangement of cytoplasmic organelles and, primarily, in disruption of the centrosome and Golgi complex colocalization. In the course of cell spreading, reorganization of intracellular compartments occurs by stages. At first, mitochondria disperse in the cytoplasm volume, then the centrosome and Golgi complex unite, and finally collapse of intermediate filaments disappears. In the presence of Na azide the establishment of intracellular compartments during cell spreading proceeds in a similar manner. However, unlike the normal conditions, here atypical intracellular compartments appear: some of these containing lysosomes and lipid inclusions and located in the perinuclear area, and others with extended cisterns of endoplasmic reticulum on the cell periphery. Addiitionally, in the presence of Na azide the density of microtubules and intermediate filaments increases. It has been suggested that changes in the cytoskeleton organization lead to an enhanced cell spreading.
Kisurina-Evgen'eva
O P
OP
Onishchenko
G E
GE
D000818:Animals; D002460:Cell Line; D018385:Centrosome / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D004847:Epithelial Cells / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D007202:Indicators and Reagents; D007668:Kidney / Q000166:cytology* / Q000648:ultrastructure; D008247:Lysosomes / Q000648:ultrastructure; D008928:Mitochondria / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure*; D019810:Sodium Azide; D013552:Swine
D007202:Indicators and Reagents; D019810:Sodium Azide