title: [Isolation and purification of eccrine sweat glands in human skin].
abstract: OBJECTIVE
  To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.
  
  
  METHODS
  Through digesting human skin with collagenase type II, cells of human eccrine sweat gland were isolated. Highly purified gland cells were obtained through transferring into the conditioned medium with a micropipette for at least three times under an inverted microscope. Primary culture was started immediately after purification. Cells could be further purified by enzyme-digestion to eradicate the fibroblasts.
  
  
  RESULTS
  Collagenase type II could digest dermal collagen with little damage to gland cells. Isolated cells from human sweat glands were adherent to the wall of culture flask, and they grew well in cultures. The problem of contamination by tissue debris and other cells such as fibroblast could be overcome.
  
  
  CONCLUSIONS
  Isolation and purification of human sweat gland cells in vitro are still facing tough problems. Gland cells are successful to isolate and cultivate without contamination.
authors.affiliation: Key Research Laboratory of Wound Repair, Burns Institute, 304th Clinical Department of General Hospital of PLA, Beijing 100037, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.forename: Hai-hong
authors.initials: HH
authors.lastname: Li
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Bai
authors.forename: Tong-zhu
authors.initials: TZ
authors.lastname: Sun
authors.forename: Shao-feng
authors.initials: SF
authors.lastname: Gu
authors.forename: Zhen-liang
authors.initials: ZL
authors.lastname: Qu
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004439:Eccrine Glands / Q000166:cytology*; D006801:Humans