[Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC].
OBJECTIVE
To establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.


METHODS
Samples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.1) as mobile phase. Detection wavelength was 285 nm.


RESULTS
Synephrine and N-methyltyramine in sample solution were well separated. Linearity of synephrine was good (r = 0.9999) in range of 0.35-11.24 microg. The average recovery was 97.1%, and RSD of repeatability was 1.9%.


CONCLUSIONS
This method can be used for quality control of Citri Reticulatae.
Li
Xian-duan
XD
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Ma
Zhi-jing
ZJ
Lin
Sheng
S
Gu
Xue-zhu
XZ
Mao
Shu-jie
SJ
D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012621:Seasons; D013578:Synephrine / Q000032:analysis*; D014439:Tyramine / Q000031:analogs & derivatives* / Q000032:analysis
C036244:methyl-4-tyramine; D013578:Synephrine; D014439:Tyramine