An ionizable chromophoric reagent for the analysis of primary amine-containing drugs by capillary electrophoresis.
We found that ofloxacin acyl chloride is a potential chromophoric reagent for labeling amino analytes for capillary electrophoresis. Ofloxacin acyl chloride has a tertiary amino function in its structure and the derivatives from ofloxacin acyl chloride reacting with amino analytes can be ionized by an acid treatment and analyzed by simple capillary zone electrophoresis. Ofloxacin acyl chloride was used to derivatize model analytes (without chromophore) of amantadine (amino drug), tranexamic acid (non-protein amino carboxylic acid), glycine, and methionine (protein amino acids). The resulting derivatives were analyzed by capillary zone electrophoresis with ultraviolet detection (300 nm). The detection limits of the analytes studied were in the range of 1.0-2.5 microM (S/N = 3, injection 3 s). The precision (relative standard deviation) and accuracy (relative error) of the method for intra- and inter-day analyses of the analytes were respectively below 4.5% and 3.9%. Application of the method to the analysis of tranexamic acid in plasma proved feasible.
Lin
Feng-Min
FM
Graduate Institute of Pharmacentical Science, Kaohsiung Medical University, Taiwan.
Kou
Hwang-Shang
HS
Wu
Shou-Mei
SM
Chen
Su-Hwei
SH
Kwan
Aij-Lie
AL
Wu
Hsin-Lung
HL
D000547:Amantadine / Q000737:chemistry / Q000302:isolation & purification; D000588:Amines / Q000737:chemistry / Q000302:isolation & purification*; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D005998:Glycine / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D008715:Methionine / Q000737:chemistry / Q000302:isolation & purification; D015242:Ofloxacin / Q000031:analogs & derivatives / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014148:Tranexamic Acid / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification
D000588:Amines; D007202:Indicators and Reagents; D014148:Tranexamic Acid; D015242:Ofloxacin; D008715:Methionine; D000547:Amantadine; D005998:Glycine