Strain-specific in vitro cytokine production profiles do not predict rat liver allograft survival.
The aim of this study was to assess whether differences in cytokine production between inbred rat strains could explain differences in liver allograft survival. Splenocytes from five different strains were cultured with Concanavalin A to determine in vitro cytokine production profiles. Strain-specific TNF-alpha, IFN-gamma, IL-6 and IL-10 responses in naive animals were not associated with survival after rat liver transplantation. To investigate whether in vitro cytokine responses changed during the allogeneic inflammatory response, Brown Norway livers were transplanted to Lewis and Pivold Virol Glaxo recipients. During the early postoperative phase IL-6 and IL-10 (Th2-like) responses were significantly up-regulated in Lewis recipients, whereas Th2-like responses were not increased in Pivold Virol Glaxo. Our results do not support the generally held view that differential in vitro cytokine responses are related to liver allograft survival but suggest that cytokine responses are affected by the allogeneic inflammatory response after liver allografting.
Warlé
Michiel C
MC
Department of Surgery, Erasmus MC Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Metselaar
Herold J
HJ
Kusters
Johannes G
JG
Zondervan
Pieter E
PE
Hop
Wim C J
WC
Segeren
Katja C A
KC
Kwekkeboom
Jaap
J
Ijzermans
Jan N M
JN
Tilanus
Hugo W
HW
D000208:Acute Disease; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D006084:Graft Rejection / Q000175:diagnosis / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016031:Liver Transplantation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D011237:Predictive Value of Tests; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology; D014184:Transplantation, Homologous; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma