[Studies on petal tissue culture of the traditional Chinese medicine Chrysanthemum morifolium].
OBJECTIVE
To set up the optimums for the petal tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.


METHODS
Petals were cut and cultured onto the same kind of media on different sides and different kinds of media on the same side, and induced to form the whole plantlet.


CONCLUSIONS
All the media used could induce callus, while the results of callus regeneration was distinct. The MS medium with KT 2 mg x L(-1) and NAA 0.2 mg x L(-1) was much better than others when callus were induced. The MS medium with KT 2 mg x L(-1) + NAA 0.2 mg x L(-1) and AgNO3 5 mg x L(-1) was the preferable medium for the bud sprouting. The frequency of plantlets regeneration which was induced by flower bud was higher than that by opening petal. The regenerated plantlets shod produced variation of leaf and plant shape.
Xue
Jian-ping
JP
Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, China. xuejp2000@yahoo.com.cn
Chang
Wei
W
Zhang
Ai-min
AM
Sheng
Wei
W
D031202:Chrysanthemum / Q000254:growth & development*; D003470:Culture Media; D035264:Flowers / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000254:growth & development*; D012835:Silver Nitrate / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators; D012835:Silver Nitrate