title: 157-nm Laser ablation of polymeric layers for fabrication of biomolecule microarrays.
abstract: A new methodology for protein microarray fabrication is proposed based on the ablation of polymer film using laser at 157 nm (F2). The polymer has been selected among others with the criterion of negligible protein adsorption. Improved results have been obtained by pretreatment of the polymer surface with an inert protein. The use of 157-nm laser radiation allowed very good depth control during the polymeric layer ablation process. In addition the importance of laser ablation at 157 nm is based on the fact that irradiated surfaces indicate limited chemical change due to the fact that laser ablation at 157 nm is only photochemical, thus avoiding excessive surface heating and damage. Results of protein microarray fabrication are presented to illustrate the viability of the proposed method.
authors.affiliation: Institute of Microelectronics, NCSR "Demokritos", Aghia Paraskevi, Athens, 15310, Greece. adouvas@imel.demokritos.gr
authors.forename: Antonios M
authors.initials: AM
authors.lastname: Douvas
authors.forename: Panagiota S
authors.initials: PS
authors.lastname: Petrou
authors.forename: Sotirios E
authors.initials: SE
authors.lastname: Kakabakos
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Misiakos
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Argitis
authors.forename: Evagelia
authors.initials: E
authors.lastname: Sarantopoulou
authors.forename: Zoe
authors.initials: Z
authors.lastname: Kollia
authors.forename: Alkiviadis C
authors.initials: AC
authors.lastname: Cefalas
mesh_terms: D007834:Lasers; D008768:Methylmethacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry / Q000528:radiation effects*; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000378:metabolism; D013499:Surface Properties
chemical_list: D008768:Methylmethacrylates; D011108:Polymers; D011506:Proteins; C045669:2,2,2-trifluoroethyl methacrylate