[Study on post-translational processing and active forms of the novel metastasis-associated protein SNC19].
To investigate the relationship between SNC19 protein and cancer metastasis.



Expression of SNC19 protein in cancer cell lines and tissues was examined by Western blot analysis using anti-SNC19 monoclonal antibody. In addition, Psectag2A-SNC19(ORF) eukaryotic expression vector was constructed and transfected into BCAP37 cells. After the target protein was expressed and purified, processing forms of SNC19 protein were further identified using anti-His mAb and each form was assayed for its gelatinase activity.



Different expression and post-translational processing of the SNC19 proteins in the cancer cell lines and intestinal tissues were detected.BCAP37 cells transfected full-length SNC19 (ORF) generated two different sized proteins in cell lysates, 120 and 75 kD; 75 kD was detected to have proteolytic activity by gelatin zymography.



SNC19 protein presents different expression and post-translational processing in the cancer cells and tissues, of which 75 kD was identified to have gelatinase activity.
Shi
Ying
Y
The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Zheng
Shu
S
Hu
Xiao-Ye
XY
Sun
Li-feng
LF
Zhang
Su-zhan
SZ
D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000091402:Prohibitins; D011499:Protein Processing, Post-Translational; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D018076:DNA, Complementary; D009363:Neoplasm Proteins; C000717331:PHB2 protein, human; D000091402:Prohibitins; D012697:Serine Endopeptidases; D018093:Gelatinases