title: A universal method for automated gene mapping.
abstract: Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.
authors.affiliation: Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. peder.zipperlen@molbio.unizh.ch
authors.forename: Peder
authors.initials: P
authors.lastname: Zipperlen
authors.forename: Knud
authors.initials: K
authors.lastname: Nairz
authors.forename: Ivo
authors.initials: I
authors.lastname: Rimann
authors.forename: Konrad
authors.initials: K
authors.lastname: Basler
authors.forename: Ernst
authors.initials: E
authors.lastname: Hafen
authors.forename: Michael
authors.initials: M
authors.lastname: Hengartner
authors.forename: Alex
authors.initials: A
authors.lastname: Hajnal
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D004331:Drosophila melanogaster / Q000235:genetics*; D017238:Genes, Helminth; D017344:Genes, Insect; D023281:Genomics; D005838:Genotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017384:Sequence Deletion; D020080:Tandem Repeat Sequences