Portable capillary electrophoresis system with potential gradient detection for separation of DNA fragments.
A portable capillary electrophoresis (CE) system with a novel potential gradient detection (PGD) was utilized to separate DNA fragments. For the first time it was demonstrated that separation of DNA fragments in polymer solution could be detected by a portable CE system integrated with PGD, with a limit of detection (LOD) comparable to that of the CE-ultraviolet (UV) method. Effects of buffer solution, sieving medium, and applied voltage were also investigated. The portable CE-PGD system shows several potential advantages, such as simplicity, cost effectiveness, and miniaturization.
Xu
Yan
Y
Department of Chemistry, National University of Singapore, Republic of Singapore.
Qin
Weidong
W
Li
Sam F Y
SF
D010584:Bacteriophage phi X 174 / Q000235:genetics; D002021:Buffers; D053938:DNA Fragmentation; D004279:DNA, Viral / Q000302:isolation & purification*; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization
D002021:Buffers; D004279:DNA, Viral