title: [A multiplex for simultaneous amplification of three Y-STR loci using modified primers].
abstract: OBJECTIVE
  To develop a multiplex method for simultaneous detection of Y-STR loci.
  
  
  METHODS
  The primers of Y-STR loci were devised for use in the simultaneous amplification of three Y-STR loci (DYS390, DYS391, DYS393).
  
  
  RESULTS
  Simultaneous amplification of the three Y-STR loci was successfully performed using the modified primers in which the fragment length of DYS391 was shortened from 279-287 bp to 142-150 bp, and at the same time, the virile specificity of DYS393 was enhanced.
  
  
  CONCLUSIONS
  The use of new-devised primers of Y-STR loci is a valid approach to rapid detection of multiple Y-STRs loci; it is worthy to be recommended in the field of forensic science.
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jia
authors.forename: Kuan-lin
authors.initials: KL
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Mei-li
authors.initials: ML
authors.lastname: Lü
authors.forename: Wei-bo
authors.initials: WB
authors.lastname: Liang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D041322:Chromosomes, Human, Y; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004247:DNA