HPLC quantification of uncarine D and the anti-plasmodial activity of alkaloids from leaves of Mitragyna inermis (Willd.) O. Kuntze.
An efficient system for the analysis of the total alkaloids extracted from leaves of Mitragyna inermis (Willd.) O. Kuntze (Rubiaceae) by HPLC using a reversed-phase column is described. The chromatographic conditions allowed the separation of indole and oxindole alkaloids in leaf extracts, and the quantification of uncarine D in samples collected in Burkina Faso and Mali. The HPLC method described was validated for its specificity, linearity and precision using an internal standard (naphthalene). The concentrations of uncarine D in various extracts were compared with their in vitro anti-plasmodial activity. The anti-proliferative activity on chloroquine-resistant strain (W2) of Plasmodium falciparum was not correlated with the concentration of uncarine D in leaves.
Fiot
Julien
J
Laboratoire de Pharmacognosie, Faculté de Pharmacie, 13385 Marseille Cedex 5, France.
Baghdikian
Béatrice
B
Boyer
Laurent
L
Mahiou
Valérie
V
Azas
Nadine
N
Gasquet
Monique
M
Timon-David
Pierre
P
Balansard
Guy
G
Ollivier
Evelyne
E
D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032065:Mitragyna / Q000737:chemistry*; D015394:Molecular Structure; D000078183:Oxindoles; D018515:Plant Leaves / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects; D015203:Reproducibility of Results
D000470:Alkaloids; D000962:Antimalarials; D000078183:Oxindoles; C104393:speciophylline