title: Purification and cDNA cloning of NADPH-dependent aldoketoreductase, involved in asymmetric reduction of methyl 4-bromo-3-oxobutyrate, from Penicillium citrinum IFO4631.
abstract: Penicillium citrinum was found to catalyze the reduction of methyl 4-bromo-3-oxobutyrate to methyl (S)-4-bromo-3-hydroxybutyrate [(S)-BHBM] with high optical purity. From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated. Escherichia coli cells overexpressing KER produced (S)-BHBM in the presence of an NADPH-regeneration system.
authors.affiliation: Organic Synthesis Research Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan. asako@sc.sumitomo-chem.co.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Asako
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Wakita
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Jun
authors.initials: J
authors.lastname: Sakai
authors.forename: Nobuya
authors.initials: N
authors.lastname: Itoh
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism
chemical_list: D018076:DNA, Complementary; D006885:Hydroxybutyrates; C496653:methyl-4-bromo-3-hydroxybutyrate; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase