[DNA and RNA random amplification polymorphism in 5-flurocytosine-resistant strains of Candida albicans from revul-vaginal candidasis].
To investigate the DNA genome and RNA expression in 5-flurocytosine-resistant strains of Candida albicans from vaginal candidasis.



Sixteen strains of Candida albicans were selected from clinically diagnosed revul-vaginal candidasis. Eight 5-flurocytosine-sensitive isolates and 8 resistant isolates were examined by France Media FUNGUS sensitive test. DNA genome was detected with random amplification polymorph DNA. RNA expression was detected with random amplification polymorph RNA method.



There were no distinct differences between 5-flurocytosine-sensitive and resistant Candida albicans in DNA genome, while RNA expression showed significant differences between 5-flurocytosine-resistant and sensitive strains.



Clinical 5-flurocytosine-resistant strains of Candida albicans from revul-vaginal candidasis may be related to phenotype changes.
Jiang
Xiu-xiu
XX
The Affiliated Obstetrics and Gynecology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.
Lu
Shi-ming
SM
D000293:Adolescent; D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D025141:Drug Resistance, Fungal; D005260:Female; D005437:Flucytosine / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D012331:RNA, Fungal / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique
D000935:Antifungal Agents; D004271:DNA, Fungal; D012331:RNA, Fungal; D005437:Flucytosine