Proteomic profiling of human stem cells derived from umbilical cord blood.
CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or instable) proteome of stem cells. All matching spots were subjected to in-gel digestion and nano-LC-MS/MS sequence analysis, from which 22 proteins were unambiguously identified.
Zenzmaier
Christoph
C
Lifecord Inc., Koerblergasse 42, A-8010 Graz, Austria.
Gesslbauer
Bernd
B
Grobuschek
Nina
N
Jandrositz
Anita
A
Preisegger
Karl-Heinz
KH
Kungl
Andreas J
AJ
D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry*
D020543:Proteome