title: In vitro analysis of the digestive enzymes amylase and alpha-glucosidase in the midguts of Locusta migratoria L. in response to the myosuppressin, SchistoFLRFamide.
abstract: We have investigated the effect of the locust myosuppressin, SchistoFLRFamide, on the activity of amylase and alpha-glucosidase in the midgut of 2-week old male locusts. Total enzyme activity in the lumen contents and tissue extracts of midguts responds to SchistoFLRFamide in a dose-dependent manner that appears to vary with the feeding state of the locust and duration of exposure to the peptide. Starvation for 24h prior to assessment alters the distribution of enzyme activity between the midgut lumen contents and tissue extracts in response to SchistoFLRFamide when compared with fed locusts. Duration of exposure to SchistoFLRFamide also alters the distribution of total amylase and alpha-glucosidase activity; as duration of exposure increases, lower concentrations of SchistoFLRFamide increase total enzyme activity in the lumen contents while decreasing total enzyme activity in the tissue extracts. We suggest that the minimum amino acid sequence in SchistoFLRFamide necessary to increase both amylase and alpha-glucosidase activity is DHVFLRFamide. We have determined that two other peptides endogenous to the locust, AFIRFamide and GQERNFLRFamide, increase amylase and alpha-glucosidase activity in midgut lumen contents.
authors.affiliation: Department of Biology, University of Toronto at Mississauga, Mississauga, Ontario, Canada L5L 1C6. shill@utm.utoronto.ca
authors.forename: Sharon R
authors.initials: SR
authors.lastname: Hill
authors.forename: Ian
authors.initials: I
authors.lastname: Orchard
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001558:Benzethonium / Q000494:pharmacology; D004064:Digestive System; D005247:Feeding Behavior; D007301:Insect Hormones / Q000502:physiology*; D048188:Locusta migratoria / Q000201:enzymology*; D008297:Male; D009479:Neuropeptides / Q000819:agonists / Q000502:physiology*; D013997:Time Factors; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; C059573:SchistoFLRFamide; D001558:Benzethonium; D000681:Amylases; D000520:alpha-Glucosidases