Ganglioside GM3 inhibits proliferation and invasion of glioma.
GM3, the simplest ganglioside, modulates cell adhesion, proliferation and differentiation in the central nervous system and exogenously added GM3 regulates cell-cell and cell-extracellular matrix adhesion and induces apoptosis. To assess the anti-tumor action of exogenous GM3, we examined its effect on the proliferation and invasion of glioma cells. Its inhibitory effect on cell proliferation was demonstrated in vitro by 3-(4,5-dimethyl-2-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay and in vitro in rats with meningeal gliomatosis whose survival was significantly prolonged by the intrathecal injection of GM3. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay revealed that GM3 induced glioma cell apoptosis in vitro and in vitro. In rat brain slice cultures, GM3 suppressed the invasion of glioma cells; this effect manifested earlier than the inhibition of cell proliferation and before apoptosis induction. Our results suggest exogenous GM3 as a potential therapeutic agent in patients with glioma requiring adjuvant therapy.
Fujimoto
Yasunori
Y
Department of Neurosurgery, Osaka University Medical School, Suita, Osaka, Japan.
Izumoto
Shuichi
S
Suzuki
Tsuyoshi
T
Kinoshita
Manabu
M
Kagawa
Naoki
N
Wada
Kouichi
K
Hashimoto
Naoya
N
Maruno
Motohiko
M
Nakatsuji
Yuji
Y
Yoshimine
Toshiki
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000473:pathology* / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005679:G(M3) Ganglioside / Q000494:pharmacology*; D005910:Glioma / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D066298:In Vitro Techniques; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D051381:Rats
D005679:G(M3) Ganglioside