title: An aqueous Chlorella extract inhibits IL-5 production by mast cells in vitro and reduces ovalbumin-induced eosinophil infiltration in the airway in mice in vivo.
abstract: An aqueous extract of the edible microalga (CP) (1), has recently been tested for its immunomodulatory effects in a human clinical trial. Here, the CP extract was dialyzed and fractionated using Sephadex G 100 chromatography. The effects of a dialyzed aqueous CP extract, fraction 2 , on mast cell mediator release in vitro and ovalbumin-induced allergic airway inflammation in vivo were examined. In vitro, treatment of mouse bone marrow-derived mast cells with 2 for 18 h significantly inhibited antigen (trinitrophenyl-BSA)-induced IL-5 production. In vivo, treatment of mice with 2 during ovalbumin sensitization and stimulation process significantly reduced eosinophil and neutrophil infiltration in the airways. Moreover, fractions obtained by size exclusion chromatography of 2 inhibited IgE-dependent cytokine GM-CSF production from human cord blood-derived mast cells. Taken together, these results suggest that 2 is composed of biopolymers with anti-allergic potential.
authors.affiliation: Ocean Nutrition Canada Ltd., Bioscience Enterprise Centre, 1721 Lower Water Street, Halifax, NS, Canada B3J 1S5. jkralovec@ocean-nutrition.com
authors.forename: J A
authors.initials: JA
authors.lastname: Kralovec
authors.forename: M R
authors.initials: MR
authors.lastname: Power
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: E
authors.initials: E
authors.lastname: Maydanski
authors.forename: H S
authors.initials: HS
authors.lastname: Ewart
authors.forename: L V
authors.initials: LV
authors.lastname: Watson
authors.forename: C J
authors.initials: CJ
authors.lastname: Barrow
authors.forename: T J
authors.initials: TJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002708:Chlorella / Q000737:chemistry*; D019587:Dietary Supplements; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D007073:Immunoglobulin E / Q000276:immunology; D015848:Interleukin-5 / Q000096:biosynthesis*; D008168:Lung / Q000187:drug effects* / Q000276:immunology*; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010047:Ovalbumin
chemical_list: D015848:Interleukin-5; D007073:Immunoglobulin E; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010047:Ovalbumin