Pathogenicity and infectivity of the Thai-strain densovirus (AThDNV) in Anopheles minimus S.L.
We report the first experimental infection of an anopheline, An. minimus s.l., with the Thai-strain densovirus, AThDNV. Two hundred first-instar larvae were raised in 2 virus concentrations; mortality in the low virus concentration was no different than that in controls (9.5% vs 7.5%, respectively). Mortality in the high virus concentration was 17.5%. Among surviving adults, infection rates were 33.3% (low concentration) and 15.5% (high concentration), as judged by PCR-screening. Infection rates did not differ between males and females. All orally-infected females transmitted densovirus to at least some of their offspring. Vertical transmission rates ranged from 25.0-53.8%. Densovirus infection did not appear to affect fecundity, either in the number of eggs laid or the number of eggs hatched.
Rwegoshora
R Theophil
RT
Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand. theophilr@hotmail.com
Kittayapong
Pattamaporn
P
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000821:virology*; D017990:Densovirus / Q000235:genetics / Q000472:pathogenicity*; D005260:Female; D005298:Fertility; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D007303:Insect Vectors / Q000821:virology; D007814:Larva / Q000821:virology*; D008288:Malaria / Q000635:transmission; D008297:Male; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D013785:Thailand