title: [Inhibitory effect of p21WAF1 on squamous cell carcinoma of larynx mediated with EGF-R targeting polypeptide gene delivery system].
abstract: OBJECTIVE
  To evaluate the efficiency of EGF-R targeted polypeptide gene delivery system and inhibitory effect of p21WAF1 on squamous cell carcinoma of larynx.
  
  
  METHODS
  The EGF-R targeting polypeptide gene delivery system was constructed and a therapeutic gene of p21WAF1 were transfected into Hep-2 cells with the new gene delivery system. The growth curve of transfected Hep-2 cells and flow cytometric analysis were applied to assess the transferring and expression of exogenous genes and inhibitory effect on Hep-2 cells.
  
  
  RESULTS
  With the transferring of p21WAF1 gene, the growth of Hep-2 cells was inhibited significantly by 79%, and the apoptosis was observed by means of flow cytometric analysis.
  
  
  CONCLUSIONS
  EGF-R targeting polypeptide gene delivery system could transfer the therapeutic gene into targeted EGF-R expressing Hep-2 cells and the expression of p21WAF1 could inhibit the growth of Hep-2 cells and induced the cells to apoptosis.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Beijing University, Beijing, 100034, China.
authors.forename: Shuifang
authors.initials: S
authors.lastname: Xiao
authors.forename: Chengyuan
authors.initials: C
authors.lastname: Wang
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Li
authors.forename: Peikun
authors.initials: P
authors.lastname: Tian
authors.forename: Jianren
authors.initials: J
authors.lastname: Gu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D007822:Laryngeal Neoplasms / Q000628:therapy*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D066246:ErbB Receptors