[Study on single nucleotide polymorphism of TLR4 in Chinese population].
OBJECTIVE
To identify the single nucleotide polymorphisms(SNPs) in the regulatory and coding regions of human Toll-like receptor 4(TLR4) gene and to search for its new genetic makers.


METHODS
The 5' flank region, exons, parts of the introns, as well as 3' flank region of TLR4 gene were sequenced to identify and characterize the SNPs in Chinese population. SNP genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism for 2 highly distributed SNPs.


RESULTS
Five novel SNPs were identified through a 4.98 kb sequencing of TLR4 gene. Among them, three were in 5'flank region, two in 3'UTR. In the sample of Han population from Chongqing, the minor allele frequencies of two highly distributed SNPs were 0.266 and 0.404 respectively.


CONCLUSIONS
Sampling analysis in Han population of Chongqing showed that the two highly distributed SNPs of TLR4 were common in Chinese population and could be used for genetic marker of TLR4 gene.
Feng
Kai
K
National Key Laboratory of Traumatic Medicine, Research Institute for Traffic Medicine, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, 400042 PR China.
Zhou
Gang-qiao
GQ
Zhai
Yun
Y
Zhu
Pei-fang
PF
Wang
Zheng-guo
ZG
He
Fu-chu
FC
Jiang
Jian-xin
JX
D000328:Adult; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D051197:Toll-Like Receptor 4 / Q000235:genetics*
C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4