title: Biotin-phenyldiazomethane conjugates as labeling reagents at phosphate in mono and polynucleotides.
abstract: Molecules 2-5 that include in their structure a biotin moiety as detectable unit and differently substituted phenyl diazo functions as reactive group were prepared as reagents for labeling the phosphate group in mono and polynucleotides. These molecules were shown to react selectively and quantitatively with the model nucleotide 3'-UMP. They were used successfully in the labeling step of DNA and RNA analysis using high-density DNA-chips (or microarrays) technology.
authors.affiliation: LEDSS-UMR5616 associé au CNRS, BP53, Université Joseph Fourier, 38041 Grenoble, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bourget
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Trévisiol
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bridon
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Kotera
authors.forename: Jean
authors.initials: J
authors.lastname: Lhomme
authors.forename: Ali
authors.initials: A
authors.lastname: Laayoun
mesh_terms: D001710:Biotin / Q000737:chemistry*; D003978:Diazomethane / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D009711:Nucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*
chemical_list: D009711:Nucleotides; D010710:Phosphates; D003978:Diazomethane; D001710:Biotin