title: Isolation of sterols from the marine fungus Corollospora lacera.
abstract: Several marine fungi collected from the waters of Prince Edward Island, Canada, were screened for the presence of natural products exhibiting antibacterial activity. Both broths and mycelia of these fungi were studied using the bioassay-guided chromatographic separation. The 4 fractions from the extract of mycelia of Corollospora lacera exhibited weak antibacterial activity and were analyzed further. From these fractions, 2 sterols (5 alpha,8 alpha-epidioxyergosterol and 22E,24R-ergosta-7,22-diene-3beta,5 alpha,6 beta-triol) and a 3:1 mixture of linoleic and oleic acids were isolated. The presence of ergosterol was confirmed in dichloromethane extracts of mycelia of every fungus in this study and this sterol was isolated from the extract of mycelium of Corollospora lacera. Two other known compounds (5-hydroxymethylfuran-2-carbaldehyde and bis(2-ethylhexyl) phthalate), were isolated from the dichloromethane extract of mycelium of Monodictys pelagica. The phthalate was reported in the literature as a metabolite isolated from the fungi, but in our study it was proven to be an artifact of the culturing and (or) extraction procedures rather than a true fungal metabolite.
authors.affiliation: Department of Chemistry, Saint Mary's University, Halifax, Canada.
authors.forename: Sabrena E
authors.initials: SE
authors.lastname: MacKenzie
authors.forename: G Soundari
authors.initials: GS
authors.lastname: Gurusamy
authors.forename: Adam
authors.initials: A
authors.lastname: Piórko
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Strongman
authors.forename: Tingmo
authors.initials: T
authors.lastname: Hu
authors.forename: Jeffrey L C
authors.initials: JL
authors.lastname: Wright
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry* / Q000302:isolation & purification*; D002170:Canada; D003470:Culture Media / Q000737:chemistry; D004051:Diethylhexyl Phthalate / Q000302:isolation & purification; D004875:Ergosterol / Q000302:isolation & purification; D019787:Linoleic Acid / Q000302:isolation & purification / Q000494:pharmacology; D025282:Mycelium / Q000737:chemistry; D019301:Oleic Acid / Q000302:isolation & purification / Q000494:pharmacology; D012623:Seawater / Q000382:microbiology*; D013261:Sterols / Q000302:isolation & purification* / Q000494:pharmacology*; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D013261:Sterols; D019301:Oleic Acid; D019787:Linoleic Acid; D004051:Diethylhexyl Phthalate; D004875:Ergosterol