title: A genotyping system capable of simultaneously analyzing >1000 single nucleotide polymorphisms in a haploid genome.
abstract: A high-throughput genotyping system for scoring single nucleotide polymorphisms (SNPs) has been developed. With this system, >1000 SNPs can be analyzed in a single assay, with a sensitivity that allows the use of single haploid cells as starting material. In the multiplex polymorphic sequence amplification step, instead of attaching universal sequences to the amplicons, primers that are unlikely to have nonspecific and productive interactions are used. Genotypes of SNPs are then determined by using the widely accessible microarray technology and the simple single-base extension assay. Three SNP panels, each consisting of >1000 SNPs, were incorporated into this system. The system was used to analyze 24 human genomic DNA samples. With 5 ng of human genomic DNA, the average detection rate was 98.22% when single probes were used, and 96.71% could be detected by dual probes in different directions. When single sperm cells were used, 91.88% of the SNPs were detectable, which is comparable to the level that was reached when very few genetic markers were used. By using a dual-probe assay, the average genotyping accuracy was 99.96% for 5 ng of human genomic DNA and 99.95% for single sperm. This system may be used to significantly facilitate large-scale genetic analysis even if the amount of DNA template is very limited or even highly degraded as that obtained from paraffin-embedded cancer specimens, and to make many unpractical research projects highly realistic and affordable.
authors.affiliation: Department of Molecular Genetics, Microbiology and Immunology/The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA.
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Wang
authors.forename: Minjie
authors.initials: M
authors.lastname: Luo
authors.forename: Irina V
authors.initials: IV
authors.lastname: Tereshchenko
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Frikker
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Cui
authors.forename: James Y
authors.initials: JY
authors.lastname: Li
authors.forename: Guohong
authors.initials: G
authors.lastname: Hu
authors.forename: Yi
authors.initials: Y
authors.lastname: Chu
authors.forename: Marco A
authors.initials: MA
authors.lastname: Azaro
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Yang
authors.forename: Manousos E
authors.initials: ME
authors.lastname: Kambouris
authors.forename: Richeng
authors.initials: R
authors.lastname: Gao
authors.forename: Weichung
authors.initials: W
authors.lastname: Shih
authors.forename: Honghua
authors.initials: H
authors.lastname: Li
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D019295:Computational Biology / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D015894:Genome, Human; D005838:Genotype; D006238:Haploidy; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism
chemical_list: D017931:DNA Primers