DNA marker mining of ILSTS035 microsatellite locus on chromosome 6 of Hanwoo cattle.
We describe tests for detecting and locating quantitative trait loci (QTL) for traits in Hanwoo cattle. From results of a permutation test to detect QTL for marbling, we selected the microsatellite locus ILSTS035 on chromosome 6 for further analysis. K-means clustering analysis applied to five traits and nine DNA markers in ILSTS035 resulted in three cluster groups. Finally we employed the bootstrap test method to calculate confidence intervals using the resampling method to find major DNA markers. We conclude that the major markers of ILSTS035 locus on chromosome 6 of Hanwoo cattle are markers 235 bp and 266 bp.
Yeo
Jung-Sou
JS
Department of Animal Science, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea.
Lee
Jea-Young
JY
Kim
Jae-Woo
JW
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D016000:Cluster Analysis; D004247:DNA / Q000235:genetics*; D005819:Genetic Markers; D007723:Korea; D018895:Microsatellite Repeats / Q000235:genetics*; D008957:Models, Genetic; D040641:Quantitative Trait Loci
D005819:Genetic Markers; D004247:DNA