Expression of cell cycle regulatory genes during primordial-primary follicle transition in the mouse ovary.
OBJECTIVE
To elucidate the genes involved in the ovarian primordial-primary follicle transition.


METHODS
Experimental animal study.


METHODS
Research unit of university.


METHODS
Day-1 and day-5 female ICR strain mice.


METHODS
We conducted forward and reverse suppression subtractive hybridization using ovarian mRNAs from day-1 and day-5 mice.


METHODS
After subtractive hybridization, the differential expression of certain genes between day-1 and day-5 ovaries was confirmed by Northern blot analysis. Differential expression of genes between primordial and primary follicles that are involved in the cell-cycle regulation was confirmed by laser capture microdissection (LCM) of follicles followed by real-time polymerase chain reaction (PCR).


RESULTS
In toto, 347 clones were sequenced and analyzed with the BLAST and RIKEN programs. Sequences of 312 clones significantly matched database entries, and 35 clones were novel. Among them, differential expression of five genes between day-1 and day-5 ovaries was confirmed by Northern blot analysis. In addition, differential expression between primordial and primary follicles of 11 more genes, particularly related to the cell-cycle regulation, was confirmed using LCM followed by real-time PCR.


CONCLUSIONS
We reported the ovarian expression of many cell-cycle regulating genes during the primordial-primary follicle transition process. Results of the present study may provide insight for further study of the molecular mechanism(s) involved in primordial-primary follicle transition.
Park
Chang-Eun
CE
CHA Research Institute, Pochon CHA University College of Medicine, Seoul, Korea.
Cha
Kwang-Yul
KY
Kim
Kyungjin
K
Lee
Kyung-Ah
KA
D000818:Animals; D000831:Animals, Newborn; D018797:Cell Cycle Proteins / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D008813:Mice, Inbred ICR; D009693:Nucleic Acid Hybridization; D006080:Ovarian Follicle / Q000166:cytology* / Q000254:growth & development / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy
D018797:Cell Cycle Proteins