title: Mineralocorticoid receptor inhibition ameliorates the transition to myocardial failure and decreases oxidative stress and inflammation in mice with chronic pressure overload.
abstract: BACKGROUND
  Although aldosterone, acting via mineralocorticoid receptors, causes left ventricular (LV) hypertrophy in experimental models of high-aldosterone hypertension, little is known about the role of aldosterone or mineralocorticoid receptors in mediating adverse remodeling in response to chronic pressure overload.
  
  
  RESULTS
  We used the mineralocorticoid receptor-selective antagonist eplerenone (EPL) to test the role of mineralocorticoid receptors in mediating the transition from hypertrophy to failure in mice with chronic pressure overload caused by ascending aortic constriction (AAC). One week after AAC, mice were randomized to regular chow or chow containing EPL (200 mg/kg per day) for an additional 7 weeks. EPL had no significant effect on systolic blood pressure after AAC. Eight weeks after AAC, EPL treatment improved survival (94% versus 65%), attenuated the increases in LV end-diastolic (3.4+/-0.1 versus 3.7+/-0.1 mm) and end-systolic (2.0+/-0.1 versus 2.5+/-0.2 mm) dimensions, and ameliorated the decrease in fractional shortening (42+/-2% versus 34+/-4%). EPL also decreased myocardial fibrosis, myocyte apoptosis, and the ratio of matrix metalloproteinase-2/tissue inhibitor of matrix metalloproteinase-2. These beneficial effects of EPL were associated with less myocardial oxidative stress, as assessed by 3-nitrotyrosine staining, reduced expression of the adhesion molecule intercellular adhesion molecule-1, and reduced infiltration by macrophages.
  
  
  CONCLUSIONS
  Mineralocorticoid receptors play an important role in mediating the transition from LV hypertrophy to failure with chronic pressure overload. The effects of mineralocorticoid receptor stimulation are associated with alterations in the interstitial matrix and myocyte apoptosis and may be mediated, at least in part, by oxidative stress and inflammation.
authors.affiliation: Cardiovascular Medicine Section, Boston University Medical Center, Boston, Mass, USA.
authors.forename: Gabriela M
authors.initials: GM
authors.lastname: Kuster
authors.forename: Eugene
authors.initials: E
authors.lastname: Kotlyar
authors.forename: Mary K
authors.initials: MK
authors.lastname: Rude
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Siwik
authors.forename: Ronglih
authors.initials: R
authors.lastname: Liao
authors.forename: Wilson S
authors.initials: WS
authors.lastname: Colucci
authors.forename: Flora
authors.initials: F
authors.lastname: Sam
mesh_terms: D000818:Animals; D001011:Aorta; D017209:Apoptosis; D001794:Blood Pressure; D048429:Cell Size; D002908:Chronic Disease; D003251:Constriction, Pathologic / Q000150:complications; D004353:Drug Evaluation, Preclinical; D000077545:Eplerenone; D005355:Fibrosis; D006333:Heart Failure / Q000209:etiology / Q000517:prevention & control*; D017379:Hypertrophy, Left Ventricular / Q000150:complications* / Q000188:drug therapy / Q000503:physiopathology; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008026:Ligation; D008297:Male; D020782:Matrix Metalloproteinases / Q000032:analysis; D051379:Mice; D000451:Mineralocorticoid Receptor Antagonists; D009205:Myocarditis / Q000188:drug therapy / Q000209:etiology; D009206:Myocardium / Q000737:chemistry / Q000473:pathology; D032383:Myocytes, Cardiac / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D011312:Pressure; D011897:Random Allocation; D018161:Receptors, Mineralocorticoid / Q000502:physiology; D013148:Spironolactone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000451:Mineralocorticoid Receptor Antagonists; D018161:Receptors, Mineralocorticoid; D019715:Tissue Inhibitor of Metalloproteinase-1; D018799:Intercellular Adhesion Molecule-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D013148:Spironolactone; C002744:3-nitrotyrosine; D014443:Tyrosine; D000077545:Eplerenone; D020782:Matrix Metalloproteinases