title: A universal plasmid library encoding all permutations of small interfering RNA.
abstract: Small interfering RNA (siRNA) is normally designed to silence preselected known genes. Such selections are inevitably prone to bias as a result of limited knowledge about the biological process, transcript identity, and functions. A library that contains all permutations of siRNA could avoid such problems. In this paper, it is shown that 5 x 10(7) siRNA-encoding plasmids can be constructed in a single tube by using vectors with two mutated RNA polymerase III promoters arranged in a convergent manner. Such a library was used to carry out genomewide screening of functional genes in a phenotype-driven manner. Multiple siRNAs that induce a significant increase of cell proliferation speed were identified.
authors.affiliation: Chinese Human Genome Center North Beijing, Beijing 100176, People's Republic of China.
authors.forename: Meihong
authors.initials: M
authors.lastname: Chen
authors.forename: Lishu
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhang
authors.forename: Xiahui
authors.initials: X
authors.lastname: Xiong
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.forename: Bing
authors.initials: B
authors.lastname: Lu
authors.forename: Claes
authors.initials: C
authors.lastname: Wahlestedt
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation; D004258:DNA Polymerase III / Q000235:genetics; D015723:Gene Library; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D004258:DNA Polymerase III