Application of preparative high-speed counter-current chromatography for separation and purification of arctiin from Fructus Arctii.
Following an initial clean-up step on the AB-8 resin (polystyrene resin, 0.3-1.25 mm: NanKai Chemical Factory, Tianjin, China), high-speed counter-current chromatography (HSCCC) was used to purify an arctiin from an extract of the fruits of the Arctium lappa L. Arctiin is a major lignan compound in the traditional Chinese medicinal herb A. lappa L. The two-phase solvent system used was composed of ethyl acetate-n-butanol-ethanol-water at an optimized volume ratio of 5:0.5:1:5 (v/v/v/v). The upper phase was used as the mobile phase in the head to tail elution mode. A total amount of 159 mg of arctiin at 98% purity was obtained from 350 mg of the crude extract (containing 49% arctiin) with 91% recovery. The preparative isolation and purification of arctiin by HSCCC was completed in 5 h in a separation. Identification of the target compound was performed by LC-electrospray ionization MS and 13C-NMR. The structure of the product was further confirmed by comparison with authentic sample (National Institute of the Control of Pharmaceutical and Biological Products, Beijing, China).
Wang
Xiao
X
Test Center, Shandong Academy of Sciences, Jinan, Shandong 250014, China.
Li
Fuwei
F
Sun
Qinglei
Q
Yuan
Jingpeng
J
Jiang
Ting
T
Zheng
Chengchao
C
D031170:Arctium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005663:Furans / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*
D005663:Furans; D005960:Glucosides; C077992:arctiin