[DNA microarray analysis of protective mechanism of buyang huanwu decoction on focal cerebral ischemia/reperfusion in rats].
OBJECTIVE
To analyse the effects of buyang huanwu decoction (BYHWT) on differentially expressed genes during cerebral ischemia/reperfusion in rats with DNA microarray.


METHODS
cDNA microarray chips containing 512 cDNAs were made by Biostar Genechip Inc. Sprague-Dawley rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) with an filament. Saline or BYHWT was given p.o. after onset of cerebral ischemia and brains were removed after 24 h of recirculation for mRNAs isolation. A differential measurment of mRNAs from post-ischemic and BYHWT treated animals was performed with microarray.


RESULTS
Up-and down-regulated genes were 69 and 80 in ischemic group. Up-and down-regulated genes were 25 and 6 in BYHWT treated group.


CONCLUSIONS
BYHWT regulates the differential expression genes after focal brain ischemia/reperfusion in rats, due to its mechanism of protecting cerebral ischemia/reperfusion injury.
Li
Tie-jun
TJ
College of Pharmacy, Second Military Medical University Shanghai 200433, China.
Qiu
Yaen
Y
Rui
Yao-cheng
YC
Li
Ting-zhao
TZ
Zhang
Wei-dong
WD
D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020869:Gene Expression Profiling; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000235:genetics*
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C460102:buyang huanwu