Selective growth-inhibiting effects of compounds identified in Tabebuia impetiginosa inner bark on human intestinal bacteria.
The growth-inhibiting activity of anthraquinone-2-carboxylic acid and lapachol identified in the inner bark of taheebo, Tabebuia impetiginosa, toward 10 human intestinal bacteria was evaluated by using a paper disk diffusion bioassay and compared to those of seven lapachol congeners (1,4-naphthoquinone, naphthazarin, menadione, lawsone, plumbagin, juglone, and dichlone) as well as two commercially available antibiotics, chloramphenicol and tetracycline. Anthraquinone-2-carboxylic acid exhibited very strong growth inhibition of Clostridium paraputrificum at 1 microg/disk while 100 microg/disk of lapachol was needed for moderate growth inhibition of the same organism. These two isolates exhibited weak inhibition of Clostridium perfringens and Escherichia coli at 100 microg/disk while no adverse effects were observed on the growth of Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Lactobacillus acidophilus, and Lactobacillus casei at 1000 microg/disk. Structure-activity relationships indicate that a methyl group in the C-2 position of 1,4-naphthoquinone derivatives might play an important role in antibacterial activity.
Park
Byeoung-Soo
BS
School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-742, Korea.
Kim
Jun-Ran
JR
Lee
Sung-Eun
SE
Kim
Kyoung Soon
KS
Takeoka
Gary R
GR
Ahn
Young-Joon
YJ
Kim
Jeong-Han
JH
D000880:Anthraquinones / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D006131:Growth Inhibitors / Q000032:analysis* / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009285:Naphthoquinones / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry*; D013329:Structure-Activity Relationship; D029663:Tabebuia / Q000737:chemistry*
D000880:Anthraquinones; D006131:Growth Inhibitors; D009285:Naphthoquinones; C498247:anthraquinone-2-carboxylic acid; C008252:lapachol