[Detection of hepatotoxic pyrrolizidine alkaloids in Ligularia Cass. with LC/MSn].
OBJECTIVE
To detect the hepatotoxic pyrrolizidine alkaloids (HPA) in the genus Ligularia Cass..


METHODS
The alkaloid extracts of Ligularia plant materials were detected and analyzed by the method of combination of TLC, and LC/MSn.


RESULTS
Among 22 species of Ligularia Cass., HPA were detected in 18 species with LC/MSn, and no HPA was detected in the remaining 4 species.


CONCLUSIONS
HPA was first detected with LC/MSn in L. tongelensis and other 15 species of Ligularia Cass.; HPA from these plants should be isolated, separated and identified and it is necessary to study the activities and toxicities of the HPA. The types and kinds of HPA from different species and sources are different, they should be detected separately.
Pu
She-ban
SB
College of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210038, China.
Xu
De-ran
DR
Zhang
Mian
M
Zhou
Hong-hua
HH
Wang
Zheng-tao
ZT
Yu
Guo-dian
GD
D019659:Asteraceae / Q000737:chemistry* / Q000145:classification; D002855:Chromatography, Thin Layer; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000737:chemistry; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D011763:Pyrrolizidine Alkaloids; C412858:otonecine; C014795:retronecine