title: [Analysis of the E gene sequences of three dengue virus type 1 isolates in Guangdong province].
abstract: OBJECTIVE
  To trace the infection sources of three dengue virus type 1 isolates found in Guangdong province, namely GD05/99, GD14/97 and GD23/95.
  
  
  METHODS
  According to the genomes of dengue virus type 1 S275 strain, a pair of primers was designed for amplification of the structural protein E gene of the isolated dengue virus type 1 strains by reverse transcriptase-PCR. The amplified gene fragment was then cloned into pMD18-T vector and sequenced.
  
  
  RESULTS
  GD14/97 and GD05/99 shared a high nucleotide homology with Cambodia (98% and 99% respectively) and may belong to the same genotype. GD23/95 had a 95% nucleotide homology with A88, higher than that with other strains. GD23\95 and A88 may belong to another genotype.
  
  
  CONCLUSIONS
  The dengue fever outbreak in Guangdong during 1999, 1997, and 1995 may originate from the viruses from different countries.
authors.affiliation: Department of Microbiology, Institute of Military Medicine of Guangzhou Command, Guangzhou 510507, China. txd1957@Sohu.com
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Tian
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Liu
authors.forename: Mei-yu
authors.initials: MY
authors.lastname: Fang
authors.forename: Lian-hua
authors.initials: LH
authors.lastname: Jiang
authors.forename: Rui-wen
authors.initials: RW
authors.lastname: Ren
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Hong
authors.forename: Gang-feng
authors.initials: GF
authors.lastname: Cheng
mesh_terms: D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C072281:E protein TH Sman, Dengue virus