[Studies on the chemical constituents from culbs of hybridized Bulbus Fritillariae Ussuriensis].
OBJECTIVE
To study the chemical constituents of hybridized Bulbus Fritillariae Ussuriensis.


METHODS
The chemical constituents were isolated by silica column chromatography and their structures were identified by physical and chemical eveidences and spectral analysis (IR, 1H-NMR, 13C-NMR, 2D-NMR, MS).


RESULTS
Seven compounds were obtained and identified as (20S,25S)5alpha, 14alpha, 17beta-cevanine-6beta-hydroxy-3-one (hupehenirine, ZF1), (20S,25S)5alpha, 14alpha, 17beta-cevanine-3beta-hydroxy-6-one (hupehenizine, ZF2), (20R,25S)5alpha, 14alpha-cevanine-3beta,20beta-dihydroxy-6-one (peiminine, verticinone, ZF3), (20S,25S)5alpha, 14alpha, 17beta-cevanine-3beta, 6beta-dihydroxy (hupehenine, ZF4), (20R,25S)5alpha, 14alpha-cevanine-3beta, 6beta, 20beta-trihydroxy (isoverticine, ZF5), (20R,25S)5alpha, 14alpha-cevanine-3beta, 6alpha, 20beta-trihydroxy (peimine, verticine, ZF6), (20S,25S)5alpha, 14alpha, 17beta-evanine-6beta-hydroxy-3beta-O-beta-D-glucoside (hupeheninoside, ZF7).


CONCLUSIONS
Compounds ZF1-7 were isolated from hybridized Bulbus Fritillariae Ussuriensis for the first time.
Ruan
Han-li
HL
School of Pharmacy of Tongji Medical College, Huazhong University of Science And Technology Wuhan, 430030, China. ruanhl@027.net
Zhang
Yong-hui
YH
Pan
Xu-chu
XC
Dong
Tao
T
Wu
Ji-zhou
JZ
D002595:Cevanes / Q000737:chemistry / Q000302:isolation & purification*; D031410:Fritillaria / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D006824:Hybridization, Genetic; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification / Q000235:genetics
D002595:Cevanes; D005960:Glucosides; C014242:verticine; C047384:hupeheninoside