Measurement of messenger RNA encoding the alpha-chain, polymeric immunoglobulin receptor, and J-chain in duodenal mucosa from dogs with and without chronic diarrhea by use of quantitative real-time reverse transcription-polymerase chain reaction assays.
OBJECTIVE
To examine the difference in expression of messenger RNA (mRNA) transcripts for polymeric immunoglobulin receptor (plgR), alpha-chain, and J-chain determined by use of quantitative real-time reverse transcription-polymerase chain reaction (QRT-PCR) assays in duodenal biopsy specimens obtained from dogs with and without chronic diarrhea.


METHODS
Biopsy specimens of the proximal portion of the duodenum were obtained endoscopically from 39 dogs evaluated because of chronic diarrhea (12 German Shepherd Dogs and 27 non-German Shepherd Dog breeds); specimens were also obtained from a control group of 7 dogs evaluated because of other gastrointestinal tract diseases and 2 dogs that were euthanatized as a result of nongastrointestinal tract disease.


METHODS
Dogs were anesthetized, and multiple mucosal biopsy specimens were obtained endoscopically at the level of the caudal duodenal flexure by use of biopsy forceps; in 2 control dogs, samples were obtained from the descending duodenum within 5 minutes of euthanasia. One-step QRT-PCR was used to quantify the level of expression of transcripts for the housekeeper gene glyceraldehyde-3-phosphate dehydrogenase, plgR, alpha-chain, and J-chain in duodenal mucosal tissue.


RESULTS
There was no significant difference in the level of expression of any transcript among non-German Shepherd Dog breeds without diarrhea (control group), non-German Shepherd Dog breeds with chronic diarrhea, and German Shepherd Dogs with chronic diarrhea. Conclusions and Clinical Relevance-Results indicated that the susceptibility of German Shepherd Dogs to chronic diarrhea is not a result of simple failure of transcription of the key genes that encode molecules involved in mucosal IgA secretion.
Peters
Iain R
IR
School of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, UK.
Helps
Chris R
CR
Calvert
Emma L
EL
Hall
Edward J
EJ
Day
Michael J
MJ
D000818:Animals; D002908:Chronic Disease; D003967:Diarrhea / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004386:Duodenum / Q000276:immunology; D005260:Female; D015870:Gene Expression; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis* / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000096:biosynthesis* / Q000235:genetics; D007137:Immunoglobulin alpha-Chains / Q000096:biosynthesis* / Q000235:genetics; D007413:Intestinal Mucosa / Q000276:immunology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D019056:Receptors, Polymeric Immunoglobulin / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D007071:Immunoglobulin A, Secretory; D007144:Immunoglobulin J-Chains; D007137:Immunoglobulin alpha-Chains; D012333:RNA, Messenger; D019056:Receptors, Polymeric Immunoglobulin