A survey of the Th2R and Th3R allelic variants in the circumsporozoite protein gene of P. falciparum parasites from western Thailand.
Allelic variation in the Plasmodium falciparum circumsporozoite protein (CS) gene has been determined by sequencing the immunodominant T-cell epitopes, Th2R and Th3R, from 95 isolates from two malaria-endemic areas in the west of Thailand. Comparison with a reference sequence revealed only non-synonymous point mutations in the two epitope regions. Point mutations were found outside these epitopes in a minority of samples, and all but four were also non-synonymous. A relatively high number of variants, 11 Th2R and 9 Th3R, were detected and comprised some that had not been previously observed. However, the Th2R*05 and the Th3R*01 allelic variants predominated, as they were found in more than 70% of the 101 sequences obtained.
Kumkhaek
Chutima
C
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Phra-ek
Kooruethai
K
Singhasivanon
Pratap
P
Looareesuwan
Sornchai
S
Hirunpetcharat
Chakrit
C
Brockman
Alan
A
Grüner
Anne Charlotte
AC
Lebrun
Nicolas
N
Rénia
Laurent
L
Nosten
François
F
Snounou
Georges
G
Khusmith
Srisin
S
D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000235:genetics*; D016778:Malaria, Falciparum / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013785:Thailand
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D018984:Epitopes, T-Lymphocyte; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan