In vitro cytotoxicity of nitric oxide-releasing sol-gel derived materials.
The cytotoxicity of bare and PU-coated nitric oxide (NO)-releasing sol-gel derived materials (sol-gels) was investigated using L929 mouse fibroblasts in both direct and indirect contact models to differentiate between the biological impact of the sol-gel matrix and NO release. The flux of NO was varied up to 150 pmol cm(-2) s(-1) using N-(6-aminohexyl)-aminopropyltrimethoxysilane (balance iso-butyltrimethoxysilane) diazeniumdiolate (NO donor)-modified sol-gels. The addition of a polyurethane (PU) outer membrane greatly improved the stability of the sol-gel matrix without significantly suppressing the NO flux. Direct contact studies demonstrated a cytotoxic effect that was dependent on the aminosilane content of the sol-gel. The use of the thin PU overcoat eliminated this effect. A direct cytotoxicity dependence of NO release for L929 fibroblasts was discovered from indirect contact studies, where 24 h exposure to NO fluxes in excess of 50 pmol cm(-2) s(-1) was cytotoxic.
Nablo
Brian J
BJ
Department of Chemistry, University of North Carolina at Chapel Hill, Venable Hall, CB#3290, Chapel Hill, NC 27599, USA.
Schoenfisch
Mark H
MH
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects* / Q000737:chemistry; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology*; D008422:Materials Testing; D051379:Mice; D009569:Nitric Oxide / Q000009:adverse effects* / Q000737:chemistry; D044367:Phase Transition; D012821:Silanes / Q000009:adverse effects* / Q000737:chemistry
D001672:Biocompatible Materials; D012821:Silanes; D009569:Nitric Oxide; C071717:trimethoxysilane