Inhibition of caspase-3 blocks long-term potentiation in hippocampal slices.
Incubation of rat hippocampal slices with the specific caspase-3 inhibitor Z-DEVD-FMK led to suppression of long-term potentiation (LTP), which developed over a period of time. Incubation of Z-DEVD-FMK for 4 h and more prevented tetanization from evoking LTP; Z-DEVD-FMK had no effect on baseline measures of synaptic plasticity and short-term plasticity (population spike amplitude, level of facilitation in conditions of paired stimuli). These data provide the first evidence for the involvement of a mechanism mediated by caspase-3 in the phenomenon of LTP.
Kudryashov
I E
IE
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow.
Yakovlev
A A
AA
Kudryashova
I V
IV
Gulyaeva
N V
NV
D000704:Analysis of Variance; D000818:Animals; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000502:physiology; D004558:Electric Stimulation / Q000379:methods; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D013997:Time Factors
D061945:Caspase Inhibitors; D009842:Oligopeptides; C110772:benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases