DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals.
The non-lipid-dependent species Malassezia pachydermatis is frequently isolated from animals. We analyzed the DNA sequences of the intergenic spacer (IGS) 1 region, which is the most variable region in the rRNA gene, of 43 M. pachydermatis strains obtained from dogs or cats. The lengths of the IGS 1 regions ranged from 552 to 898 bp and, based on the nucleotide sequence, these IGS 1 regions were divided into three major groups with 10 subtypes. Group 1 (552-601 bp long) was characterized by the short sequence repeat (CAGCA)n and had four to 14 repeats, and Group 3 (749-898 bp long), which included the neotype strain of M. pachydermatis, was characterized by the sequence (CAGCATAACATAACACACAACA)n in the IGS1 region. Group 2 possessed partial sequences of both Groups 1 and 3. Each group shared only 41.7-55.4% similarity in the IGS1 region with the other groups. The internal transcribed spacer (ITS) region and D1/D2 26S rDNA in the rRNA gene were also sequenced for representative strains in each IGS group. The groups were distinguished by both ITS (698-712 bp long including 5.8S rDNA) and D1/D2 26S rDNA (624 bp long) sequences with sequence similarities of 91.7-96.0% and 99.7-99.0%, respectively. Our results indicate that the sequence of the IGS region of M. pachydermatis has a remarkable intraspecies diversity, compared with ITS or D1/D2 26S rDNA, and that multiple genotypic strains of M. pachydermatis colonize animal skin.
Sugita
T
T
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. sugita@mypharm.ac.jp
Takeo
K
K
Hama
K
K
Virtudazo
E
E
Takashima
M
M
Nishikawa
A
A
Kucsera
J
J
Dorogi
J
J
Komori
S
S
Nakagaki
K
K
Vollekova
A
A
Slavikova
E
E
Farkas
V
V
D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics*; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D014644:Genetic Variation; D008290:Malassezia / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S