Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold.
Cartilage engineered from mesenchymal stem cells (MSCs) requires a scaffold to keep the cells in the cartilage defect and to act as a support for inducing hyaline cartilage formation. We developed a novel three-dimensional special poly-lactic-glycolic acid (PLGA) scaffold that provided structural support and stimulated repair. Three-dimensional PLGA scaffolds seeded with cultured MSCs were transplanted into large defects in rabbit knees and analyzed histologically at 4 and 12 weeks after the operation. Our findings showed that in the engineered cartilage with the PLGA scaffold, the defects were filled with smooth, shiny white tissue macroscopically and hyaline-like cartilage histologically at 12 weeks after the transplantation. The structure of the novel PLGA scaffolds provided architectural support for the differentiation of progenitor cells and demonstrated successful induction of in vivo chondrogenesis.
Uematsu
Kota
K
Department of Orthopaedic Surgery, Nara Medical University, 840, Shijou-cho, Kashihara-city, Nara 634-8522, Japan. kuematsu@nmu-gw.narmed-u.ac.jp
Hattori
Koji
K
Ishimoto
Yoshiyuki
Y
Yamauchi
Jun
J
Habata
Takashi
T
Takakura
Yoshinori
Y
Ohgushi
Hajime
H
Fukuchi
Takeshi
T
Sato
Masao
M
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002356:Cartilage / Q000254:growth & development / Q000293:injuries / Q000473:pathology* / Q000601:surgery*; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000473:pathology*; D048091:Guided Tissue Regeneration / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology
D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid