title: Intraductal tubular neoplasms of the pancreas: histogenesis and differentiation.
abstract: OBJECTIVE
  Intraductal neoplasms of the pancreas are generally referred to as intraductal papillary mucin-producing neoplasms (IPMNs), according to the WHO classification system. Herein, we report that morphologic and immunohistochemical features of intraductal tubular carcinoma (ITC) are quite different from those of intraductal papillary mucinous carcinoma (IPMC).
  
  
  METHODS
  We analyzed histogenesis and differentiation of ITC by light microscopy and immunohistochemistry.
  
  
  RESULTS
  Histologically, ITC was characterized as an intraductal nodular appearances with a monotonous tubular growth pattern without papillary projection. ITC showed de novo-like appearance without sequential progression usually observed in IPMC, suggesting that ITC is a homogeneous neoplasm. Cuboidal tumor cells in ITC resembled normal pancreatic duct epithelia, and the characteristic growth pattern of ITC replaced that of normal pancreatic duct epithelium. Immunohistochemically, ITC cells were positive for MUC-1 on the apical side of the cell membrane. In contrast to ITC cells, IPMC cells were negative for MUC-1, and ductal adenocarcinoma cells were strongly positive for MUC-1, as was the stroma around the cancer. The immunohistochemical staining pattern of DUPAN-2 resembled that of MUC-1. Interestingly, localization of MUC-1 and DUPAN-2 staining in ITC cells was similar to that in normal pancreatic ductules. ITC cells were negative for MUC-2 and MUC-5AC. In contrast, most IPMC cells were positive for MUC-2 and MUC-5AC.
  
  
  CONCLUSIONS
  Based on our histologic and immunohistochemical findings, the intraductal pancreatic neoplasm (IPN) can be classified into 2 groups: IPN with gastrointestinal differentiation and IPN with pancreatic duct differentiation. Our present data indicated that ITC cells may arise directly from duct epithelia without progression and possessed pancreatic duct differentiation. On the basis of our data, we suggest that classification of pancreatic neoplasms in the WHO and The Armed Forces Institute of Pathology (AFIP) systems should be reconsidered.
authors.affiliation: Division of Gastroenterology, Showa University Fujigaoka Hospital, Yokohama, Japan. tajiri@showa-university-fujigaoka.gr.jp
authors.forename: Takuma
authors.initials: T
authors.lastname: Tajiri
authors.forename: Genshu
authors.initials: G
authors.lastname: Tate
authors.forename: Tomoko
authors.initials: T
authors.lastname: Inagaki
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kunimura
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Inoue
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Mitsuya
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshiba
authors.forename: Toshio
authors.initials: T
authors.lastname: Morohoshi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D002288:Adenocarcinoma, Mucinous / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism / Q000473:pathology*; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009077:Mucins / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D009077:Mucins