Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus.
To identify the function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome (SARS) coronavirus (CoV), we analyzed the protein-protein interaction among HAb18G/CD147, cyclophilin A (CyPA), and SARS-CoV structural proteins by coimmunoprecipitation and surface plasmon resonance analysis. Although none of the SARS-CoV proteins was found to be directly bound to HAb18G/CD147, the nucleocapsid (N) protein of SARS-CoV was bound to CyPA, which interacted with HAb18G/CD147. Further research showed that HAb18G/CD147, a transmembrane molecule, was highly expressed on 293 cells and that CyPA was integrated with SARS-CoV. HAb18G/CD147-antagonistic peptide (AP)-9, an AP of HAb18G/CD147, had a high rate of binding to 293 cells and an inhibitory effect on SARS-CoV. These results show that HAb18G/CD147, mediated by CyPA bound to SARS-CoV N protein, plays a functional role in facilitating invasion of host cells by SARS-CoV. Our findings provide some evidence for the cytologic mechanism of invasion by SARS-CoV and provide a molecular basis for screening anti-SARS drugs.
Chen
Zhinan
Z
Department of Cell Biology, the Fourth Military Medical University, Xi'an, China.
Mi
Li
L
Xu
Jing
J
Yu
Jiyun
J
Wang
Xianhui
X
Jiang
Jianli
J
Xing
Jinliang
J
Shang
Peng
P
Qian
Airong
A
Li
Yu
Y
Shaw
Peter X
PX
Wang
Jianwei
J
Duan
Shumin
S
Ding
Jin
J
Fan
Chunmei
C
Zhang
Yang
Y
Yang
Yong
Y
Yu
Xiaoling
X
Feng
Qiang
Q
Li
Biehu
B
Yao
Xiying
X
Zhang
Zheng
Z
Li
Ling
L
Xue
Xiaoping
X
Zhu
Ping
P
D015703:Antigens, CD / Q000502:physiology*; D051926:Basigin; D002460:Cell Line; D000086462:Coronavirus Nucleocapsid Proteins; D021984:Cyclophilin A / Q000502:physiology; D003588:Cytopathogenic Effect, Viral / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D019590:Nucleocapsid Proteins / Q000502:physiology; D011485:Protein Binding; D045473:Severe acute respiratory syndrome-related coronavirus / Q000472:pathogenicity*; D015678:Viral Structural Proteins / Q000502:physiology*
D015703:Antigens, CD; C497886:BSG protein, human; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D015678:Viral Structural Proteins; D051926:Basigin; D021984:Cyclophilin A