Semicarbazide-functionalized Si(111) surfaces for the site-specific immobilization of peptides.
The covalent attachment of semicarbazide-functionalized layers to hydrogen-terminated Si(111) surfaces is reported. The surface modification, based on the photoinduced hydrosilylation of a Si(111) surface with protected semicarbazide-functionalized alkenes, was investigated by means of X-ray photoelectron spectroscopy (XPS), contact angle measurements, and atomic force microscopy (AFM). The removal of the protecting group yielded a semicarbazide-terminated monolayer which was reacted with peptides bearing a glyoxylyl group for site-specific alpha-oxo semicarbazone ligation.
Coffinier
Y
Y
Institut d'Electronique et de Microélectronique et de Nanotechnologie, IEMN, (CNRS, UMR 8520) Département ISEN, 41 bd Vauban, 59046 Lille Cedex, France.
Olivier
C
C
Perzyna
A
A
Grandidier
B
B
Wallart
X
X
Durand
J-O
JO
Melnyk
O
O
Stiévenard
D
D
D004133:Diminazene / Q000031:analogs & derivatives* / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013053:Spectrophotometry; D014867:Water / Q000737:chemistry
D010455:Peptides; D014867:Water; C003915:diminazene aceturate; D004133:Diminazene; D012825:Silicon