title: Clone-array pooled shotgun mapping and sequencing: design and analysis of experiments.
abstract: This paper studies sequencing and mapping methods that rely solely on pooling and shotgun sequencing of clones. First, we scrutinize and improve the recently proposed Clone-Array Pooled Shotgun Sequencing (CAPSS) method, which delivers a BAC-linked assembly of a whole genome sequence. Secondly, we introduce a novel physical mapping method, called Clone-Array Pooled Shotgun Mapping (CAPS-MAP), which computes the physical ordering of BACs in a random library. Both CAPSS and CAPS-MAP construct subclone libraries from pooled genomic BAC clones.
authors.affiliation: Département d'informatique et de recherche opérationnelle, Université de Montréal, CP 6128 succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada. csuros@iro.umontreal.ca
authors.forename: Miklós
authors.initials: M
authors.lastname: Csürös
authors.forename: Bingshan
authors.initials: B
authors.lastname: Li
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Milosavljevic
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D004331:Drosophila melanogaster / Q000235:genetics; D016678:Genome; D015203:Reproducibility of Results; D012107:Research Design; D016415:Sequence Alignment