The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway.
Protein ubiquitination and deubiquitination are dynamic processes implicated in the regulation of numerous cellular pathways. Monoubiquitination of the Fanconi anemia (FA) protein FANCD2 appears to be critical in the repair of DNA damage because many of the proteins that are mutated in FA are required for FANCD2 ubiquitination. By screening a gene family RNAi library, we identify the deubiquitinating enzyme USP1 as a novel component of the Fanconi anemia pathway. Inhibition of USP1 leads to hyperaccumulation of monoubiquitinated FANCD2. Furthermore, USP1 physically associates with FANCD2, and the proteins colocalize in chromatin after DNA damage. Finally, analysis of crosslinker-induced chromosomal aberrations in USP1 knockdown cells suggests a role in DNA repair. We propose that USP1 deubiquitinates FANCD2 when cells exit S phase or recommence cycling after a DNA damage insult and may play a critical role in the FA pathway by recycling FANCD2.
Nijman
Sebastian M B
SM
Division of Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
Huang
Tony T
TT
Dirac
Annette M G
AM
Brummelkamp
Thijn R
TR
Kerkhoven
Ron M
RM
D'Andrea
Alan D
AD
Bernards
René
R
D000818:Animals; D029681:Arabidopsis Proteins; D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D002869:Chromosome Aberrations; D004260:DNA Repair; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005199:Fanconi Anemia / Q000201:enzymology* / Q000209:etiology* / Q000235:genetics; D052236:Fanconi Anemia Complementation Group D2 Protein; D015723:Gene Library; D006801:Humans; D051379:Mice; D016685:Mitomycin / Q000633:toxicity; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D025801:Ubiquitin / Q000378:metabolism; D064570:Ubiquitin-Specific Proteases
D029681:Arabidopsis Proteins; D002843:Chromatin; C499905:FANCD2 protein, human; C499904:Fancd2 protein, mouse; D052236:Fanconi Anemia Complementation Group D2 Protein; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D025801:Ubiquitin; D016685:Mitomycin; D010450:Endopeptidases; C579228:USP1 protein, human; D064570:Ubiquitin-Specific Proteases; C579227:Usp1 protein, mouse; D046988:Proteasome Endopeptidase Complex