title: Biocompatible channels for field-flow fractionation of biological samples: correlation between surface composition and operating performance.
abstract: Biocompatible methods capable of rapid purification and fractionation of analytes from complex natural matrices are increasingly in demand, particularly at the forefront of biotechnological applications. Field-flow fractionation is a separation technique suitable for nano-sized and micro-sized analytes among which bioanalytes are an important family. The objective of this preliminary study is to start a more general approach to field-flow fractionation for bio-samples by investigation of the correlation between channel surface composition and biosample adhesion. For the first time we report on the use of X-ray photoelectron spectroscopy (XPS) to study the surface properties of channels of known performance. By XPS, a polar hydrophobic environment was found on PVC material commonly used as accumulation wall in gravitational field-flow fractionation (GrFFF), which explains the low recovery obtained when GrFFF was used to fractionate a biological sample such as Staphylococcus aureus. An increase in separation performance was obtained first by conditioning the accumulation wall with bovine serum albumin and then by using the ion-beam sputtering technique to cover the GrFFF channel surface with a controlled inert film. XPS analysis was also employed to determine the composition of membranes used in hollow-fiber flow field-flow fractionation (HF FlFFF). The results obtained revealed homogeneous composition along the HF FlFFF channel both before and after its use for fractionation of an intact protein such as ferritin.
authors.affiliation: Dipartimento di Chimica G. Ciamician, Università di Bologna, Via Selmi 2, 40126, Bologna, Italy. barbara.roda@unibo.it
authors.forename: Barbara
authors.initials: B
authors.lastname: Roda
authors.forename: Nicola
authors.initials: N
authors.lastname: Cioffi
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Ditaranto
authors.forename: Andrea
authors.initials: A
authors.lastname: Zattoni
authors.forename: Sonia
authors.initials: S
authors.lastname: Casolari
authors.forename: Dora
authors.initials: D
authors.lastname: Melucci
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Reschiglian
authors.forename: Luigia
authors.initials: L
authors.lastname: Sabbatini
authors.forename: Antonio
authors.initials: A
authors.lastname: Valentini
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Zambonin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D005293:Ferritins / Q000737:chemistry; D006112:Gravitation; D006736:Horses; D013057:Spectrum Analysis; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D001672:Biocompatible Materials; D005293:Ferritins