Characterization of Candida albicans colony morphological mutants and their hybrids by means of RAPD-PCR, isoenzyme analysis and pathogenicity analysis.
A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutants. Out of the isolated 10 clones, 2 stable hybrid clones were chosen and characterized: clone VI. 1M produced rough colonies containing a new, extended cell type (never observed in natural isolates), exhibited unipolar budding, did not form a germ tube, and possessed 12 chromosomal bands. All other features (antifungal and stress sensitivity, adhesion ability, pathogenicity, and isoenzyme and RAPD patterns) were similar to those of mycelial mutant M5. In contrast, the characteristics of clone VI.9S were similar to those of morphological mutant M2.
Novák
A
A
Department of General and Environmental Microbiology, Faculty of Science, University of Pécs, 7624 Pécs, Hungary.
Vágvölgyi
C
C
Emody
L
L
Pesti
M
M
D000818:Animals; D001483:Base Sequence; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D006824:Hybridization, Genetic; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D009154:Mutation; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique; D014774:Virulence / Q000235:genetics
D004271:DNA, Fungal; D007527:Isoenzymes