Co-expression of the Na(+)/H(+)-antiporter and H(+)-ATPase genes of the salt-tolerant yeast Zygosaccharomyces rouxii in Saccharomyces cerevisiae.
We cloned two genes from the salt-tolerant yeast Zygosaccharomyces rouxii: ZrSOD2 for the cell membrane Na(+)/H(+)-antiporter and ZrPMA1 for the cell membrane H(+)-ATPase. The products of these genes play cooperative roles in the salt-tolerance of Z. rouxii, and the function of the ZrPMA1 product is regulated at the transcription level. We constructed a yeast expression vector that is able to co-express the ZrSOD2 and ZrPMA1 genes. Single expression of ZrSOD2 was effective in conferring salt-tolerance, and although a slight synergic effect was observed with co-expression of ZrSOD2 and ZrPMA1, the usefulness of this co-expression is likely to be minimal with regard to salt-tolerance.
Watanabe
Yasuo
Y
Department of Biological Resources, Faculty of Agriculture, National University Corporation Ehime University, Matsuyama, Ehime 790-8566, Japan. watanabe@agr.ehime-u.ac.jp
Oshima
Naoko
N
Tamai
Youichi
Y
D015966:Gene Expression Regulation, Fungal; D007218:Industrial Microbiology / Q000379:methods; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D020068:Zygosaccharomyces / Q000235:genetics*
D029701:Saccharomyces cerevisiae Proteins; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride; D006180:Proton-Translocating ATPases