title: Isolation and characterization of temperate bacteriophages of Clostridium difficile.
abstract: The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages phiC2, phiC5, phiC6, and phiC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of phiC2 showed nucleotide homology to the sequenced C. difficile strain CD630.
authors.affiliation: Queen Elizabeth II Medical Centre, The University of Western Australia, Nedlands, Western Australia. micgs@nus.edu.sg
authors.forename: Shan
authors.initials: S
authors.lastname: Goh
authors.forename: Thomas V
authors.initials: TV
authors.lastname: Riley
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016360:Clostridioides difficile / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology; D006801:Humans; D008242:Lysogeny; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014775:Virus Activation
chemical_list: D004279:DNA, Viral