[Determination of aristolochic acid A in Radix Aristolociae and Herba Asari by RP-HPLC].
OBJECTIVE
To develop a HPLC method to determine the contents of aristolochic A in aristolochia debilis and Asarun spp..


METHODS
Methanol-water-formic acid extracts were separated on an Alltech C18 column with methanol-water-acetic acid (68:32:1) as mobile phase. The flow rate was 1.0 mL x min(-1). UV detection wavelength was 390 nm. Column temperature was 35 degrees C.


RESULTS
Aristolochic acid A was separated well. The relationship of injection amounts and peak areas was linear (r = 0.9999) the range of 0.12-1.89 microg x g(-1) and the recovery rate was 101.8% (n = 5). 11 samples of aristolochia debilis which bought from different areas in China were determined, and the contents of aristolochic acid A varied from 0.9 to 2 mg x g(-1). The difference of the contents in Asarum spp. was obvious. The highest is 0.35, and aristolochic acid A couldn't be detected in one sample.
Jiang
Xu
X
Institute of Chinese Materia Medica, China Academy of Traditonal Chinese Medicine, Beijing 100700, China.
Wang
Zhi-min
ZM
You
Li-shuan
LS
Dai
Li-ping
LP
Ding
Guang-zhi
GZ
D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis*; D030023:Asarum / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D034341:Aristolochic Acids; C000228:aristolochic acid I