A simple and effective method to improve intrasplenic rat hepatocyte transplantation.
Transplanted hepatocytes integrate, survive, and express their specific functions in the liver parenchyma. The aim of this study was to determine whether a large number of hepatocytes could move from the spleen to the liver when the cells are injected together with sodium nitroprusside, and if the improved hepatocyte migration may be related with portal vein dilatation. Wistar rats were transplanted in the spleen with fluorescent-labeled hepatocytes alone or together with sodium nitroprusside. At 1, 3, 6, and 24 h after the transplant, the liver from recipient animals was removed and morphometric analyses were performed. Portal and arterial pressures were also measured immediately after intrasplenic injection of a solution of sodium nitroprusside, hepatocytes alone, or hepatocytes plus sodium nitroprusside. Intrasplenically injected sodium nitroprusside produced a transient drop in arterial pressure and a sustained reduction in portal pressure. During hepatocyte transplantation it increased the number of transplanted cells migrating to the liver after 3 h. Sodium nitroprusside simultaneously injected with hepatocytes in the spleen allowed more cells to migrate into the liver of the host animal without risk in animal survival.
Sigot
Valeria
V
Biología Molecular, Dto Cs Biológicas, Facultad de Cs Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, Santa Fe, Argentina.
Mediavilla
María G
MG
Furno
Graciela
G
Rodríguez
Joaquín V
JV
Guibert
Edgardo E
EE
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D008107:Liver Diseases / Q000628:therapy*; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology / Q000627:therapeutic use; D017082:Portal Pressure / Q000187:drug effects / Q000502:physiology; D011169:Portal Vein / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D013154:Spleen / Q000166:cytology / Q000502:physiology / Q000601:surgery*; D016896:Treatment Outcome; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology / Q000627:therapeutic use
D014665:Vasodilator Agents; D009599:Nitroprusside