title: Dopamine D5 receptor modulates male and female sexual behavior in mice.
abstract: BACKGROUND
  Dopamine exerts its actions through at least five receptor (DAR) isoforms. In female rats, D5 DAR may be involved in expression of sexual behavior. We used a D5 knockout (D5KO) mouse to assess the role of D5 DAR in mouse sexual behavior. Both sexes of D5KO mice are fertile and exhibit only minor disruptions in exploratory locomotion, startle, and prepulse inhibition responses.
  
  
  OBJECTIVE
  This study was conducted to characterize the sexual behavior of male and female D5KO mice relative to their WT littermates.
  
  
  METHODS
  Female WT and D5KO littermates were ovariectomized and given a series of sexual behavior tests after treatment with estradiol benzoate (EB) and progesterone (P). Once sexual performance was optimal the dopamine agonist, apomorphine (APO), was substituted for P. Male mice were observed in pair- and trio- sexual behavior tests. To assess whether the D5 DAR is involved in rewarding aspects of sexual behavior, WT and D5KO male mice were tested for conditioned place preference.
  
  
  RESULTS
  Both WT and D5KO females can display receptivity after treatment with EB and P, but APO was only able to facilitate receptivity in EB-primed WT, not in D5KO, mice. Male D5KO mice display normal masculine sexual behavior in mating tests. In conditioned preference tests, WT males formed a conditioned preference for context associated with either intromissions alone or ejaculation as the unconditioned stimulus. In contrast, D5KO males only showed a place preference when ejaculation was paired with the context.
  
  
  CONCLUSIONS
  In females, the D5 DAR is essential for the actions of dopamine on receptivity. In males, D5 DAR influences rewarding aspects of intromissions. Taken together, the work suggests that the D5 receptor mediates dopamine's action on sexual behavior in both sexes, perhaps via a reward pathway.
authors.affiliation: Neuroscience Graduate Program, University of Virginia Medical School, Charlottesville, VA 22908, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Kudwa
authors.forename: E
authors.initials: E
authors.lastname: Dominguez-Salazar
authors.forename: D M
authors.initials: DM
authors.lastname: Cabrera
authors.forename: D R
authors.initials: DR
authors.lastname: Sibley
authors.forename: E F
authors.initials: EF
authors.lastname: Rissman
mesh_terms: D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D003213:Conditioning, Psychological; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011374:Progesterone / Q000494:pharmacology; D012201:Reward; D012726:Sexual Behavior, Animal
chemical_list: D011374:Progesterone; D004958:Estradiol; D001058:Apomorphine