title: Chimeric human immunodeficiency virus type 1 virions that contain the simian immunodeficiency virus nef gene are cyclosporin A resistant.
abstract: We have previously shown that human immunodeficiency virus type 1 (HIV-1) virions which have their own nef gene deleted and are trans complemented to contain HIV-2 or simian immunodeficiency virus (SIV) Nef become resistant to treatment with cyclosporin A. To expand and confirm these studies, we have tested an HIV-1 isolate in which the HIV-1 nef gene has been replaced by the nef gene from SIV in a multiround infectivity assay using more physiologically relevant cell types. Our results confirm that HIV-1 virions that contain SIV nef can replicate in a cyclophilin-independent fashion.
authors.affiliation: Dept. of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, 720 Westview Dr. SW, Atlanta, GA 30310, USA. powellm@msm.edu
authors.forename: Mahfuz
authors.initials: M
authors.lastname: Khan
authors.forename: Lingling
authors.initials: L
authors.lastname: Jin
authors.forename: Lesa
authors.initials: L
authors.lastname: Miles
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Bond
authors.forename: Michael D
authors.initials: MD
authors.lastname: Powell
mesh_terms: D000818:Animals; D002460:Cell Line; D002678:Chimera / Q000235:genetics; D016572:Cyclosporine / Q000494:pharmacology*; D024882:Drug Resistance, Viral / Q000235:genetics; D015754:Genes, nef; D005816:Genetic Complementation Test; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D016572:Cyclosporine