title: Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos.
abstract: The present study investigated the expression of receptors for glycosaminoglycans (GAGs) and the effect of GAGs supplementation on development of porcine IVF embryos. Total RNA was prepared from oocytes, 2-, 4- and 8-cell embryos, morulae and blastocysts. The expression of hyaluronic acid receptor (CD44) and heparin (HP) interacting protein (HIP) was determined using RT-PCR and Southern blot analysis. The CD44 and HIP mRNA were detected from in vitro matured oocytes and all stages of pre-implantation embryos. The IVF embryos were cultured in modified NCSU-23 medium supplemented with various concentrations (0, 0.1, 0.5 or 1.0 mg/mL) of hyaluronic acid (HA) or heparin. Supplementing with 0.5 mg/mL HA significantly increased total cell number compared to other experimental groups, due to increase in trophectoderm cells. Supplementing with 1.0 mg/mL, HP significantly increased blastocyst formation rate compared to the control group. Supplementing media, in which IVF embryos were cultured with 0.5 mg/mL HA + 1.0 mg/mL HP, significantly increased blastocyst formation rate compared to the control group. In conclusion, the present study demonstrated the expression of HA and HP receptors and the embryotrophic effect of HA or HP on porcine IVF embryos.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Hye-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Gab-Sang
authors.initials: GS
authors.lastname: Lee
authors.forename: Sang-Hwan
authors.initials: SH
authors.lastname: Hyun
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Nam
authors.forename: So-Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Yeon-Woo
authors.initials: YW
authors.lastname: Jeong
authors.forename: Sue
authors.initials: S
authors.lastname: Kim
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Kim
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Kang
authors.forename: Byeong-Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Woo-Suk
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D001755:Blastocyst; D015139:Blotting, Southern; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects*; D015870:Gene Expression; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006493:Heparin / Q000008:administration & dosage; D018960:Hyaluronan Receptors / Q000235:genetics; D006820:Hyaluronic Acid / Q000008:administration & dosage; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000196:embryology*
chemical_list: D003470:Culture Media; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C086020:glycosaminoglycan receptor; C037680:heparin receptor; D006820:Hyaluronic Acid; D006493:Heparin