title: Chloramphenicol and expression of multidrug efflux pump in Enterobacter aerogenes.
abstract: Chloramphenicol has been reported to act as an inducer of the multidrug resistance in Escherichia coli. A resistant variant able to grow on plates containing 64 microg/ml chloramphenicol was obtained from the Enterobacter aerogenes ATCC 13048-type strain. Chloramphenicol resistance was due to an active efflux of this antibiotic and it was associated with resistance to fluoroquinolones and tetracycline, but not to aminoglycoside or beta-lactam antibiotics. MDR in the chloramphenicol-resistant variant is linked to the overexpression of the major AcrAB-TolC efflux system. This overexpression seems unrelated to the global Mar and the local AcrR regulatory pathways.
authors.affiliation: Enveloppe Bactérienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Ghisalberti
authors.forename: Muriel
authors.initials: M
authors.lastname: Masi
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Pagès
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Chevalier
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000187:drug effects / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics / Q000494:pharmacology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002701:Chloramphenicol / Q000493:pharmacokinetics* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000502:physiology*; D021902:Enterobacter aerogenes / Q000145:classification / Q000187:drug effects* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008657:Metabolic Clearance Rate; D013045:Species Specificity
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; C493251:AcrAB-TolC protein, Salmonella enterica; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D002701:Chloramphenicol