Increase in degraded collagen type II in synovial fluid early in the rabbit meniscectomy model of osteoarthritis.
OBJECTIVE
The objective of this study was to determine whether collagen type II breakdown products in synovial fluid (SF), detected by an enzyme-linked immunoassay, represent a useful marker for early events in osteoarthritis (OA) in the rabbit medial meniscectomy model.


METHODS
Complete medial meniscectomy was performed on the right knee joints of 32 rabbits. Balanced groups of rabbits were then sacrificed at 2, 4, 8, and 12 weeks post-surgery. An additional 8 unoperated and 11 sham-operated animals served as controls. SF lavages were performed on right and left knee joints of the same animals at sacrifice. The proteolytic epitope of type II collagen was monitored using an enzyme-linked immunoassay.


RESULTS
Macroscopically visible surface fibrillation and focal erosions appeared as early as 2 weeks after meniscectomy in the femorotibial joint (P<0.01). OA developed gradually during the later observation period, and then predominantly on the medial tibial plateau and medial femur. Significant histological alterations in cartilage, including a loss of proteoglycans, surface irregularities, and clefts, were detected at 2 weeks after meniscectomy (P<0.01). Collagen type II epitope levels in SF lavage samples were elevated peaking at 2 weeks after meniscectomy (P<0.02). Levels decreased at later time points, but they were still raised at 12 weeks (P< or =0.05). Highly significant correlations were found between the SF collagen type II epitope levels and the macroscopic and microscopic scoring results (Spearman rho correlation coefficient, macroscopy-collagen type II epitope r=0.222, P=0.025; microscopy-collagen type II epitope r=0.436, P< or =0.01).


CONCLUSIONS
In this rabbit model of medial meniscectomy, levels of type II collagen fragments in SF appear to provide a useful marker of the early degenerative changes.
Lindhorst
E
E
Department of Surgery, Osteoarthritis Research Unit, Johann Wolfgang Goethe-Universität Frankfurt/Main, 60590 Frankfurt/Main, Germany. lindhorst@em.uni-frankfurt.de
Wachsmuth
L
L
Kimmig
N
N
Raiss
R
R
Aigner
T
T
Atley
L
L
Eyre
D
D
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002358:Cartilage, Articular / Q000378:metabolism / Q000473:pathology; D024043:Collagen Type II / Q000032:analysis / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D006614:Hindlimb; D007596:Joints / Q000378:metabolism / Q000473:pathology; D008297:Male; D008592:Menisci, Tibial / Q000601:surgery; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D011509:Proteoglycans / Q000032:analysis; D011817:Rabbits; D013582:Synovial Fluid / Q000378:metabolism*
D015415:Biomarkers; D024043:Collagen Type II; D000939:Epitopes; D011509:Proteoglycans