Taming the spindle for containing the chromosomes.
Checkpoint controls are critical for coordination of cell cycle events, especially during exposure to perturbations or stresses. The DNA replication checkpoint is activated in S phase in response to replication stresses that impede fork progression. Mec1 and Rad53 are critical effectors of this control pathway; they maintain the integrity of stalled replication forks and prevent premature segregation of unreplicated chromosomes. It has long been thought that the checkpoint inhibits precocious segregation of chromosomes by preventing early onset of mitosis. However, recent evidence suggests that the replication checkpoint thwarts untimely chromosome separation not by inhibiting mitotic entry but by directly regulating spindle dynamics. These findings raise a number of issues which may require a revisit to the well-trodden territories of cell cycle regulation.
Krishnan
Vaidehi
V
Institute of Molecular and Cell Biology, 138673, Singapore.
Surana
Uttam
U
D064199:Cdc20 Proteins; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D064447:Checkpoint Kinase 2; D002875:Chromosomes / Q000648:ultrastructure*; D004261:DNA Replication; D015966:Gene Expression Regulation, Fungal; D047908:Intracellular Signaling Peptides and Proteins; D018386:Kinetochores / Q000378:metabolism; D064246:Mad2 Proteins; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D016196:S Phase; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D004718:Saccharomycetales; D008941:Spindle Apparatus
C577495:CDC20 protein, S cerevisiae; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; C577695:MAD2 protein, S cerevisiae; D064246:Mad2 Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D064447:Checkpoint Kinase 2; C088937:MEC1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C067022:RAD53 protein, S cerevisiae