Expression cloning of a variety of novel protein kinases in Lotus japonicus.
To investigate protein kinases expressed in Lotus japonicus, a cDNA expression library of the root-nodule of L. japonicus was immunologically screened with monoclonal antibodies directed to a highly conserved region in protein serine/threonine kinases (Ser/Thr kinases). Among 178 positive clones obtained from the lambdaZAPII cDNA library, 164 clones were found to encode novel proteins possessing the subdomain VIB sequences characteristic of Ser/Thr kinases. By phylogenetic analysis on the basis of deduced amino acid sequences, the isolated clones could be classified into five different families of Ser/Thr kinases : the SnRK family, GSK-3 family, Ndr kinase family, Ark family, and receptor kinase family. These results suggest that this expression cloning using the kinase-specific antibodies will provide new clues for investigations of a wide variety of known and novel protein kinases in higher plants.
Kameshita
Isamu
I
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan. kamesita@ag.kagawa-u.ac.jp
Nishida
Tetsuyuki
T
Nakamura
Satoko
S
Sugiyama
Yasunori
Y
Sueyoshi
Noriyuki
N
Umehara
Yosuke
Y
Nomura
Mika
M
Tajima
Shigeyuki
S
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D000070116:Lotus / Q000235:genetics*; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D017346:Protein Serine-Threonine Kinases