title: Unsaturated diether lipids in the psychrotrophic archaeon Halorubrum lacusprofundi.
abstract: The major phospholipids of Halorubrum lacusprofundi grown at 25 degrees C were archaeol phosphatidylglycerol, archaeol phosphatidylglycerylsulphate and archaeol phosphatidylglycerylphosphate methyl ester. Glycolipids included a monoglycosyl archaeol and the sulphate ester of a diglycosyl archaeol. Cultures grown at 12 degrees C contained the same suite of phospho- and glycolipids, with the addition of a series of unsaturated analogues with up to six double bonds. The patterns of unsaturation were similar for all the phospholipid series, but a different pattern occurred in the glycolipids. The analytical techniques used in this study allow facile detection of unsaturated archaeal cell membrane lipids that are degraded by commonly used chemical derivatization procedures.
authors.affiliation: CSIRO Marine Research, GPO Box 1538, Hobart, Tasmania 7001, Australia. john.gibson@utas.edu.au
authors.forename: John A E
authors.initials: JA
authors.lastname: Gibson
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Miller
authors.forename: Noel W
authors.initials: NW
authors.lastname: Davies
authors.forename: Glen P
authors.initials: GP
authors.lastname: Neill
authors.forename: David S
authors.initials: DS
authors.lastname: Nichols
authors.forename: John K
authors.initials: JK
authors.lastname: Volkman
mesh_terms: D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification*; D006017:Glycolipids / Q000032:analysis* / Q000302:isolation & purification; D006216:Halobacteriaceae / Q000737:chemistry* / Q000254:growth & development; D013058:Mass Spectrometry; D008563:Membrane Lipids / Q000032:analysis / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis* / Q000302:isolation & purification; D013696:Temperature
chemical_list: D005231:Fatty Acids, Unsaturated; D006017:Glycolipids; D008563:Membrane Lipids; D010743:Phospholipids