[Sensitivity of phenylarsineoxide-induced mitochondrial permeability transition pore opening in the heart of old rats during intermittent hypoxic training].
We studied the sensitivity of mitochondrial permeability transition pore (MPTP) opening to its inductor-phenylarsineoxide (PAO) in adult (6 months), old rat heart (24 months) and in old rat heart under conditions of intermittent hypoxic training (IHT). We defined the sensitivity of MPTP opening on two parameters: the alterations in mitochondrial swelling and the release ofmitochondrial substances (mitochondrial factor). It was shown that mitochondria of old rat heart are more sensitive to PAO which caused opening of cyclosporin-sensitive MPTP and MPTP-dependent factor release, in comparison with those of adult rat heart mitochondria. One of the causes of increased sensitivity of MPTP opening to PAO is development of an oxidative stress with age that was accompanied by increase of an active metabolite of oxygen IHT on PAO-induced MPTP-opening and MPTP-dependent factor release from old rat heart mitochondria. IHT also reduced the content of H2O2 and *OH in old rat hearts. IHT can be used as protective procedure preventing MPTP opening in aging and, probably, in a numerous chronic pathological states of organism under oxidative stress.
Rudyk
O V
OV
Vavilova
H L
HL
Strutyns'ka
N A
NA
Kotsiuruba
A V
AV
Sahach
V F
VF
D000222:Adaptation, Physiological; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001152:Arsenicals / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D000860:Hypoxia / Q000503:physiopathology*; D007473:Ion Channels / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D008933:Mitochondrial Swelling / Q000502:physiology; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
D001152:Arsenicals; D007473:Ion Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; C029341:oxophenylarsine