Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid).
Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for both neurofilament and MAP-2 with well-organized neurites and somatodendritic localization. The presence of PDL with LN or FN on the PLGA films was essential for increased neural cell growth. Also, PLGA films coated with either PDL/LN or PDL/FN mixtures had higher neurite outgrowth and regular differentiation.
Han
Dong-Wook
DW
Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong,Seodaemun-gu, 120-752, Seoul, Korea.
Sub Lee
Min
M
Park
Bong Joo
BJ
Kim
Jeong Koo
JK
Park
Jong-Chul
JC
D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006016:Glycolates / Q000737:chemistry*; D007150:Immunohistochemistry; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D016501:Neurites; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D013499:Surface Properties
D006016:Glycolates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid