[Absorption of zedoary oil in rat intestine using in situ single pass perfusion model].
OBJECTIVE
To study the absorption of zedoary oil in intestine of rat.


METHODS
In situ single pass perfusion model was used and the concentrations of three components in perfusate were determined by HPLC in combination with diode array detection.


RESULTS
The P(app) s of curcumol, curdione and germacrone were all low and had no significant difference (P > 0.05) at zedoary oil concentration of 0.4, 0.8 and 1.2 mg x mL(-1) in transmucosal fluid or in four different regions of intestine of rat [duodenum, jejunum, ileum, colon]. The absorption rates of germacrone and curdione were faster than curcumol's in this study.


CONCLUSIONS
The zedoary oil concentration in transmucosal fluid had no significant effect on the P(app) s within the scope of 0.4-1.2 mg x mL(-1). The absorption of curcumol, curdione and germacrone showed the passive diffusion process, and didn't contain a special absorption window.
You
Jian
J
Shenyang Pharmacutical University, Shenyang 110015, China.
Li
Qing-po
QP
Yu
Ying-wei
YW
Cui
Fu-de
FD
D000818:Animals; D001692:Biological Transport; D003106:Colon / Q000378:metabolism; D030024:Curcuma / Q000737:chemistry; D004386:Duodenum / Q000378:metabolism; D007082:Ileum / Q000378:metabolism; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism; D008297:Male; D010477:Perfusion; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000493:pharmacokinetics*; D045788:Sesquiterpenes, Germacrane / Q000302:isolation & purification / Q000493:pharmacokinetics*
D010938:Plant Oils; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C048393:germacrone; C022801:curcumol