title: Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect.
abstract: Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry.
authors.affiliation: Biomedical Proteomics Research Group, Central Clinical Chemistry Laboratory, Geneva University Hospitals, Geneva, Switzerland. alexander.scherl@medecine.unige.ch
authors.forename: Alexander
authors.initials: A
authors.lastname: Scherl
authors.forename: Catherine G
authors.initials: CG
authors.lastname: Zimmermann-Ivol
authors.forename: Joel
authors.initials: J
authors.lastname: Di Dio
authors.forename: Ali R
authors.initials: AR
authors.lastname: Vaezzadeh
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Binz
authors.forename: Michel
authors.initials: M
authors.lastname: Amez-Droz
authors.forename: Roland
authors.initials: R
authors.lastname: Cochard
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Sanchez
authors.forename: Matthias
authors.initials: M
authors.lastname: Glückmann
authors.forename: Denis F
authors.initials: DF
authors.lastname: Hochstrasser
mesh_terms: D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004553:Electric Conductivity; D006046:Gold / Q000737:chemistry*; D008567:Membranes, Artificial; D010449:Peptide Mapping / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011506:Proteins; D006046:Gold