Stimulation of MCF-7 tumor progression in athymic nude mice by 17beta-estradiol induces WISP-2/CCN5 expression in xenografts: a novel signaling molecule in hormonal carcinogenesis.
There was 100% solid tumor formation following inoculation of MCF-7 cells. However, MCF-7 tumor progression was significantly greater in the mice exposed to 17beta-estradiol (17beta-E2) compared to unexposed mice. WISP-2/CCN5 mRNA expression was correspondingly increased in 17beta-E2 exposed MCF-7 tumors compared to unexposed xenografts. Moreover, estrogen exposure followed by anti-estrogen tamoxifen treatment drastically inhibited the tumor growth and WISP-2 expression in nude mice. Therefore, the study suggests that higher WISP-2/CCN5 expression by estrogen may be associated with the estrogen-induced growth of MCF-7 tumors in vivo. Finally, overexpression of WISP-2/CCN5 may be considered as a prognostic marker of estrogen-sensitive tumor growth.
Ray
Gibanananda
G
Cancer Research Unit, Research Division 151, V.A. Medical Center, 4801 Linwood Blvd., Kansas City, MO 64128, USA.
Banerjee
Snigdha
S
Saxena
Neela K
NK
Campbell
Donald R
DR
Van Veldhuizen
Peter
P
Banerjee
Sushanta K
SK
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D055511:CCN Intercellular Signaling Proteins; D018450:Disease Progression; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D011379:Prognosis; D012097:Repressor Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D055511:CCN Intercellular Signaling Proteins; C413323:CCN5 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D004958:Estradiol