Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana.
A novel Arabidopsis thaliana gene (AtNADK-1) was identified based on its response to radiation and oxidative stress. Levels of AtNADK-1 mRNA increase eight-fold following exposure to ionising radiation and are enhanced three-fold by treatment with hydrogen peroxide. The gene also appears to be differentially regulated during compatible and incompatible plant-pathogen interactions in response to Pseudomonas syringae pv. tomato. The full-length AtNADK-1 cDNA encodes a 58-kDa protein that shows high sequence homology to the recently defined family of NAD(H) kinases. Recombinant AtNADK-1 utilises ATP to phosphorylate both NAD and NADH, showing a two-fold preference for NADH. Using reverse genetics, we demonstrate that AtNADK-1 deficient plants display enhanced sensitivity to gamma irradiation and to paraquat-induced oxidative stress. Our results indicate that this novel NAD(H) kinase may contribute to the maintenance of redox status in Arabidopsis thaliana.
Berrin
Jean-Guy
JG
Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire de Bioénergétique Cellulaire, CEA Cadarache, Bât 156, 13108 Saint-Paul Lez Durance, France.
Pierrugues
Olivier
O
Brutesco
Catherine
C
Alonso
Béatrice
B
Montillet
Jean-Luc
JL
Roby
Dominique
D
Kazmaier
Michael
M
D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000528:radiation effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D044224:Pseudomonas syringae; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000872:Anthocyanins; D029681:Arabidopsis Proteins; D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D006861:Hydrogen Peroxide; D010770:Phosphotransferases; C500558:NADK-1 protein, Arabidopsis; D017853:Phosphotransferases (Alcohol Group Acceptor)