mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis.
The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from Trichinella spiralis-infected mice was examined using the reverse transcription PCR assay. Fragments of skeletal muscles were also subjected to the immunohistochemical reaction using specific anti-iNOS monoclonal antibodies followed by Dako-Ark test. mRNA for iNOS measured on day 21 after infection was expressed in the muscular phase of trichinellosis. Positive immunostaining for iNOS occurred in infiltrating mononuclear cells around the encapsulated larvae. iNOS-positive cells could be traced from the 21st day post infection (dpi); on 42 dpi and 90 dpi most cells expressed iNOS. By assessing expression of protein and its mRNA it can be concluded that iNOS is active in the pathology of skeletal muscle tissue in experimental trichinellosis.
Boczoń
Krystyna
K
Department of Biology and Medical Parasitology, University of Medical Sciences, Poznań, Poland. kboczon@amp.edu.pl
Wandurska-Nowak
Elzbieta
E
Wierzbicki
Andrzej
A
Frydrychowicz
Magdalena
M
Mozer-Lisewska
Iwona
I
Zeromski
Jan
J
D000818:Animals; D015870:Gene Expression; D007150:Immunohistochemistry; D008264:Macrophages / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000469:parasitology / Q000473:pathology*; D019001:Nitric Oxide Synthase / Q000032:analysis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017160:Trichinella spiralis / Q000472:pathogenicity; D014235:Trichinellosis / Q000201:enzymology* / Q000469:parasitology / Q000473:pathology*
D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse