title: Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2.
abstract: Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) alpha interacting protein by yeast two-hybrid screening. In addition to ER alpha, BCAS2 also interacted with ER beta, TR beta, PR, and PPAR gamma in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ER alpha but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.
authors.affiliation: Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
authors.forename: Yiwei Tony
authors.initials: YT
authors.lastname: Zhu
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Chang
authors.forename: Anjana V
authors.initials: AV
authors.lastname: Yeldandi
authors.forename: M Sambasiva
authors.initials: MS
authors.lastname: Rao
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D047628:Estrogen Receptor alpha / Q000737:chemistry / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: C120624:BCAS2 protein, human; D047628:Estrogen Receptor alpha; D009363:Neoplasm Proteins