Analytical performance and workflow evaluation of the Roche E170 modular immunoassay analyzer in a pediatric setting.
OBJECTIVE
To evaluate the analytical performance of the Roche E170 modular analytics immunoassay analyzer and assess its impact on workflow efficiency and ability to consolidate workstations in a pediatric setting.


METHODS
Analytical performance of eleven common immunoassays was assessed. Total imprecision was determined using Roche PreciControl Universal controls, Bio-Rad Lyphochek Immuno Plus, Anemia controls, and a human serum pool. Method comparison was performed with approximately 100 patient specimens. High dose hook effect, sample carryover, and results comparison between the two measuring channels were evaluated. For the workflow study, the time required for sample and reagent handling, instrument preparation, and hands-on time were assessed.


RESULTS
Correlation coefficients with existing methods ranged from 0.941 to 0.999. Biases of -19% to 70% were observed. Total imprecision ranged from 1.1 to 7.6%. No sample carryovers were encountered. Results from both measuring channels were comparable.


CONCLUSIONS
E170 is suitable for use in a pediatric setting. The analytical performance is acceptable and gives equivalent results to our existing systems. The precision is comparable and acceptable. Some improvement in efficiency, workflow, cost saving, and consolidation of workstations is possible. Significant workflow improvements can only be realized when integrated with the chemistry modules.
Wan
Betty
B
Division of Clinical Biochemistry, The Hospital for Sick Children, Department of Paediatric Laboratory Medicine, 555 University Avenue, Toronto, Canada M5G 1X8.
Augustin
Roy
R
Chan
Man Khun
MK
Leblond
John
J
Verjee
Zulfikarali
Z
Adeli
Khosrow
K
D001322:Autoanalysis / Q000295:instrumentation; D017598:Efficiency, Organizational; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007753:Laboratories / Q000458:organization & administration; D010372:Pediatrics; D012680:Sensitivity and Specificity; D016526:Workload