A fluorimetric method for the determination of nucleic acids using Ce(IV) and sodium triphosphate.
A novel and stable fluorimetric method was established for the determination of nucleic acids. The proposed method is based on the reduction by nucleic acids of Ce(IV) to fluorescent Ce(III). The fluorescence intensity can be greatly increased by sodium triphosphate. The enhanced fluorescence intensity is proportional to the concentration of nucleic acids in the range 4.2 x 10(-8)-4.2 x 10(-6) g/mL for fish sperm DNA and 5.0 x 10(-8)-6.5 x 10(-6) g/mL for yeast RNA, and the detection limits (S/N = 3) are 13.5 ng/mL and 45 ng/mL, respectively. The reaction mechanism of the hydrolytic scission of nucleic acids by Ce(IV) is discussed.
Wu
Xia
X
Key Laboratory of Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, People's Republic of China.
Yang
Jinghe
J
Sun
Changxia
C
Sun
Shuna
S
Guo
Changying
C
Chen
Yanjing
Y
D000818:Animals; D002563:Cerium / Q000737:chemistry*; D005399:Fishes / Q000378:metabolism; D008297:Male; D009696:Nucleic Acids / Q000032:analysis*; D010084:Oxidation-Reduction; D011122:Polyphosphates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013094:Spermatozoa / Q000737:chemistry; D015003:Yeasts / Q000737:chemistry
D009696:Nucleic Acids; D011122:Polyphosphates; D002563:Cerium; C005692:triphosphoric acid