title: Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde.
abstract: Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.
authors.affiliation: Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA.
authors.forename: Priscilla H
authors.initials: PH
authors.lastname: Fernandes
authors.forename: Manorama
authors.initials: M
authors.lastname: Kanuri
authors.forename: Lubomir V
authors.initials: LV
authors.lastname: Nechev
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Harris
authors.forename: R Stephen
authors.initials: RS
authors.lastname: Lloyd
mesh_terms: D000447:Aldehydes / Q000633:toxicity*; D000818:Animals; D001345:Autoradiography; D019556:COS Cells; D002522:Chlorocebus aethiops; D018736:DNA Adducts / Q000737:chemistry / Q000187:drug effects / Q000633:toxicity*; D005822:Genetic Vectors / Q000235:genetics; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D014752:Vinyl Chloride / Q000633:toxicity*
chemical_list: D000447:Aldehydes; D018736:DNA Adducts; D009841:Oligonucleotides; C012796:2-butenal; D014752:Vinyl Chloride