A collagenase-targeted multiplex PCR assay for identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus.
A multiplex PCR assay using three collagenase-targeted primer pairs for the species-specific detection of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus was developed. The results highlight the species specificity of the three primer sets designed. Because of the increasing importance of Vibrio spp. in human foodborne diseases, molecular approaches for routine microbial screening and monitoring of clinical, environmental, and food samples also have become more important. The results of this study indicate that the gene coding for collagenase should be used as an alternative molecular target to discriminate among the three Vibrio species.
Di Pinto
Angela
A
Dipartimento di Sanità e Benessere degli Animali, Facoltà di Medicina Veterinaria, Università degli Studi di Ban, Valenzano (Ba), Italy. a.dipinto@veterinaria.uniba.it
Ciccarese
Giuseppina
G
Tantillo
Giuseppina
G
Catalano
Domenico
D
Forte
Vito Tony
VT
D017931:DNA Primers; D005506:Food Contamination / Q000032:analysis*; D003012:Microbial Collagenase / Q000737:chemistry*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D044149:Vibrio alginolyticus / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D014734:Vibrio cholerae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D017931:DNA Primers; D003012:Microbial Collagenase