[Colonic expression of gamma-interferon and interleukin-10 in Crohn's disease and ulcerative colitis].
OBJECTIVE
The aim of this study was to investigate mucosal expression of INF-gamma and IL-10 in patients with Crohn's disease (CD) or ulcerative colitis (UC).


METHODS
Fourteen patients with CD and 11 patients with ulcerative colitis participated and 7 healthy subjects were also included. Study of the mucosal expression of INF-gamma and IL-10 was conducted using biopsies from healthy and damaged colons, using the inverse transcription and genetic amplification (RT-PCR) technique in real time (Taqman). Our results were expressed as ratio between messenger cytokine (mRNA) levels and ribosomal RNA level of a reference molecule (rRNA 18S), then multiplied by 108.


RESULTS
In the cases of Crohn's disease, the mucosa expressed increased INF-gamma and IL-10 compared with controls (respective medians: 23.03 vs. 1.87 p=0.04 and 20.61 vs. 2.13 p=0.08). A strong positive correlation was found in the mucosal expression of IL-10 and INF-gamma during CD (r=0.9 p<0.0001). In contrast, in patients with UC, the expression of INF-gamma and IL-10 were comparable to those observed in the controls (7.18 vs. 2.18 p=0.36 and 3.66 vs. 1.87 p=0.44).


CONCLUSIONS
During Crohn's disease, the expression of both IL-10 and INF-gamma was increased and strongly correlated, compared with the controls.
Kallel
L
L
Service de gastroentérologie A, Hôpital la Rabta, Tunis, Tunisie.
Boubaker
J
J
Louzir
H
H
Ben Ahmed
M
M
Sassi
A
A
Boubaker
S
S
Zouari
B
B
Filali
A
A
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D016022:Case-Control Studies; D002648:Child; D003093:Colitis, Ulcerative / Q000276:immunology / Q000473:pathology*; D003106:Colon / Q000737:chemistry* / Q000276:immunology; D003113:Colonoscopy; D003424:Crohn Disease / Q000276:immunology / Q000473:pathology*; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D005260:Female; D015870:Gene Expression / Q000235:genetics* / Q000276:immunology; D020869:Gene Expression Profiling; D006801:Humans; D018928:Immunity, Mucosal / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D014158:Transcription, Genetic / Q000235:genetics / Q000276:immunology
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D016753:Interleukin-10; D007371:Interferon-gamma