Prototype of immunochromatographic assay strips using colloidal CdTe nanocrystals as biological luminescent label.
Colloidal semiconductor nanocrystals have attracted considerable attention as a novel biological luminescent label. The bioinorganic conjugates of luminescent CdTe nanocrystals and protein, including CdTe/BSA (bovine serum albumin) and CdTe/MAB (mouse monoclonal antibody against hepatitis B surface antigen), were formed via electrostatic/coordination self-assembly. Pure CdTe nanocrystals, CdTe/BSA and CdTe/MAB were used in the immunochromatographic assay experiments, respectively. And the results indicated that CdTe nanocrystals could be used and developed as a novel label with good stability, high sensitivity and facile determination of several analytes in immunochromatographic assay strips.
Li
Jun
J
College of Chemistry, Jinlin University, Changchun 130023, PR China.
Zhao
Kui
K
Hong
Xia
X
Yuan
Hang
H
Ma
Lan
L
Li
Jinghong
J
Bai
Yubai
Y
Li
Tiejin
T
D000818:Animals; D000911:Antibodies, Monoclonal; D002104:Cadmium; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D049329:Nanostructures; D012709:Serum Albumin; D013053:Spectrophotometry; D013691:Tellurium
D000911:Antibodies, Monoclonal; D012709:Serum Albumin; D002104:Cadmium; D013691:Tellurium