[Phenotypic evaluation to differentiate Candida albicans from Candida dubliniensis].
This study evaluated the phenotypic tests used to differentiate Candida albicans from Candida dubliniensis. A total of 55 isolates from vaginal secretions, oral cavity and hemoculture were studied. They were originally identified as C. albicans, based on their morphological and physiological characteristics. These isolates were tested for colony color development on CHROMagar Candida medium, growth at 45 degrees C on Sabouraud Dextrose agar, lipolytic activity on Tween 80 Agar medium and colony morphology and chlamydoconidia formation on Staib agar medium. Of the 55 isolates studied, seven yielded one or more phenotypic characteristics suggestive of Candida dubliniensis. These isolates were tested by PCR with specific primers for Candida dubliniensis and API ID 32. The seven isolates were confirmed as Candida albicans. All of these finding indicate that DNA based tests should be used for definitive identification of Candida dubliniensis.
Mesa
Luz Mila
LM
Cátedra de Micología, Escuela de Bioanálisis, Facultad de Medicina, Universidad de Zulia, Maracaibo, Venezuela. lmilamesac@cantv.net
Arcaya
Noris
N
Cañas
Omaira
O
Machado
Yazmin
Y
Calvo
Belinda
B
D001769:Blood / Q000382:microbiology; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D002177:Candidiasis / Q000382:microbiology*; D002180:Candidiasis, Oral / Q000382:microbiology; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology; D003116:Color; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D005260:Female; D016469:Fungemia / Q000382:microbiology; D006801:Humans; D009055:Mouth / Q000382:microbiology; D016533:Mycological Typing Techniques / Q000379:methods*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014621:Vagina / Q000382:microbiology
D003470:Culture Media; D004271:DNA, Fungal