[Effects of adipose tissue extract on rat skin regeneration in vitro].
OBJECTIVE
To investigate the effects of adipose tissue extract on growth of rat skin in vitro.


METHODS
Neonatal rat skin (area of 2 mm x 2 mm) was cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% adipose tissue extract and 10% phosphate buffered solution (PBS). After being cultured for 6 days, the area of skin was measured.


RESULTS
Skin in both groups grew in area. With the addition of adipose tissue extract, the skin measured (8.43+/-1.40) mm2 in area after 6 days, and it was approximately 2 times of that of PBS group, in which the skin area was (4.18+/-1.11) mm2. The difference was statistically significant (P<0.05).


CONCLUSIONS
Adipose tissue extract promote skin growth, which may be attributed to the presence of some growth factors in the extract.
Lu
Gang
G
Key Research Laboratory of Wound Repair, Burns Institute, 304th Clinical Department of General Hospital of PLA, Beijing 100037, China.
Fu
Xiao-bing
XB
Li
Hai-hong
HH
Chen
Wei
W
Ma
Xiu-ling
XL
Sun
Tong-zhu
TZ
D000273:Adipose Tissue; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects*; D012867:Skin / Q000187:drug effects*; D013552:Swine; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts