title: Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes.
abstract: In this paper we report on the metabolic response of human hepatocytes grown on polyethersulfone membranes surface modified with a plasma-deposited acrylic acid coating and RGD peptide covalently immobilized through a "spacer arm" molecule. The modified surfaces were characterized by means of X-ray photoelectron spectroscopy and water contact angle measurements. The performance of modified and unmodified membranes was evaluated by assessing the expression of liver specific and biotransformation functions of human hepatocytes. Diclofenac, a non-steroidal anti-inflammatory drug, was used to investigate the biotransformation functions. Surface-modified membranes elicit specific cellular responses and induce hepatocytes to enhance the synthesis rate of albumin and urea, particularly in the presence of diclofenac. Also the biotransformation functions were expressed at high levels.
authors.affiliation: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci cubo 17/C, 87030 Rende (CS), Italy. l.debartolo@itm.cnr.it
authors.forename: Loredana
authors.initials: L
authors.lastname: De Bartolo
authors.forename: Sabrina
authors.initials: S
authors.lastname: Morelli
authors.forename: Linda C
authors.initials: LC
authors.lastname: Lopez
authors.forename: Lidietta
authors.initials: L
authors.lastname: Giorno
authors.forename: Carla
authors.initials: C
authors.lastname: Campana
authors.forename: Simona
authors.initials: S
authors.lastname: Salerno
authors.forename: Maria
authors.initials: M
authors.lastname: Rende
authors.forename: Pietro
authors.initials: P
authors.lastname: Favia
authors.forename: Loredana
authors.initials: L
authors.lastname: Detomaso
authors.forename: Roberto
authors.initials: R
authors.lastname: Gristina
authors.forename: Riccardo
authors.initials: R
authors.lastname: d'Agostino
authors.forename: Enrico
authors.initials: E
authors.lastname: Drioli
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000096:biosynthesis; D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D019164:Liver, Artificial; D008422:Materials Testing; D008567:Membranes, Artificial; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014508:Urea / Q000378:metabolism
chemical_list: D000418:Albumins; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D014508:Urea