Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect.
Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry.
Scherl
Alexander
A
Biomedical Proteomics Research Group, Central Clinical Chemistry Laboratory, Geneva University Hospitals, Geneva, Switzerland. alexander.scherl@medecine.unige.ch
Zimmermann-Ivol
Catherine G
CG
Di Dio
Joel
J
Vaezzadeh
Ali R
AR
Binz
Pierre-Alain
PA
Amez-Droz
Michel
M
Cochard
Roland
R
Sanchez
Jean-Charles
JC
Glückmann
Matthias
M
Hochstrasser
Denis F
DF
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004553:Electric Conductivity; D006046:Gold / Q000737:chemistry*; D008567:Membranes, Artificial; D010449:Peptide Mapping / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011506:Proteins; D006046:Gold