Association of SEEK1 and psoriatic arthritis in two distinct Canadian populations.
OBJECTIVE
To examine the relationship between SNP +39604 in SEEK1 and psoriatic arthritis (PsA) in two distinct Canadian populations.


METHODS
103 patients with PsA and 105 ethnically matched controls from Newfoundland and 202 patients with PsA and 100 controls from Ontario were studied. Patients and controls were genotyped for SNP +39604 of SEEK1 by time of flight mass spectrometry, using the Sequenom platform. Genomic DNA was amplified by the Dynal RELI SSO HLA-Cw* typing kit for HLA-C typing.


RESULTS
The frequency of the minor SEEK1(T) allele in subjects with PsA and controls was 48.5% and 32.4%, respectively (odds ratio (OR) = 2.0; p = 0.017), in the Newfoundland population and 46.5% and 38.0%, respectively (OR = 1.4; p = 0.16), in the Ontario population. Although SEEK1 is associated with PsA, particularly in the Newfoundland population, multivariate analysis showed that SEEK1 does not seem to be a further susceptibility factor if the HLA-Cw*0602 status is already known. No association was noted between SEEK1(T) allele and onset of psoriasis, PsA, or arthritis pattern.


CONCLUSIONS
SEEK1 is associated with PsA in the Newfoundland founder population. This association is probably due to linkage disequilibrium between SEEK1 and HLA-Cw*0602 in this population.
Rahman
P
P
St Clare's Mercy Hospital, Memorial University of Newfoundland, 1 South-154 LeMarchant Rd, St John's, Newfoundland, Canada A1C-5B8. prahman@mun.ca
Butt
C
C
Siannis
F
F
Farewell
V T
VT
Peddle
L
L
Pellett
F J
FJ
Schentag
C
C
Gladman
D D
DD
D000328:Adult; D000483:Alleles; D015535:Arthritis, Psoriatic / Q000235:genetics*; D005260:Female; D018703:Founder Effect; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D009523:Newfoundland and Labrador; D009864:Ontario; D020641:Polymorphism, Single Nucleotide; D011506:Proteins / Q000235:genetics*
C484393:PSORS1C1 protein, human; D011506:Proteins