An enzyme-amplified diffraction-based immunoassay.
We have previously shown that a gold-conjugated secondary label can be used to reduce the limit of detection in a diffraction-based assay by more than 40-fold. We now show that by using a combination of a peroxidase-conjugated secondary label and a precipitating substrate the limit of detection in a diffraction-based assay can be reduced by more than 1000-fold. The response to secondary enhancement was linear for concentrations from 50 to 2000 pg/mL of antidigoxin.
Loo
Richard W
RW
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Canada M5S 3H6. rloo@chem.utoronto.ca
Tam
Pui L
PL
Goh
Jane Betty
JB
Goh
M Cynthia
MC
D000818:Animals; D002417:Cattle; D004077:Digoxin / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D009195:Peroxidase / Q000378:metabolism*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000378:metabolism
D012710:Serum Albumin, Bovine; D004077:Digoxin; D009195:Peroxidase