Temperature-dependent variations and intraspecies diversity of the structure of the lipopolysaccharide of Yersinia pestis.
Yersinia pestis spread throughout the Americas in the early 20th century, and it occurs predominantly as a single clone within this part of the world. However, within Eurasia and parts of Africa there is significant diversity among Y. pestis strains, which can be classified into different biovars (bv.) and/or subspecies (ssp.), with bv. orientalis/ssp. pestis most closely related to the American clone. To determine one aspect of the relatedness of these different Y. pestis isolates, the structure of the lipopolysaccharide (LPS) of four wild-type and one LPS-mutant Eurasian/African strains of Y. pestis was determined, evaluating effects of growth at mammalian (37 degrees C) or flea (25 degrees C) temperatures on the structure and composition of the core oligosaccharide and lipid A. In the wild-type clones of ssp. pestis, a single major core glycoform was synthesized at 37 degrees C whereas multiple core oligosaccharide glycoforms were produced at 25 degrees C. Structural differences occurred primarily in the terminal monosaccharides. Only tetraacyl lipid A was made at 37 degrees C, whereas at 25 degrees C additional pentaacyl and hexaacyl lipid A structures were produced. 4-Amino-4-deoxyarabinose levels in lipid A increased with lower growth temperatures or when bacteria were cultured in the presence of polymyxin B. In Y. pestis ssp. caucasica, the LPS core lacked D-glycero-D-manno-heptose and the content of 4-amino-4-deoxyarabinose showed no dependence on growth temperature, whereas the degree of acylation of the lipid A and the structure of the oligosaccharide core were temperature dependent. A spontaneous deep-rough LPS mutant strain possessed only a disaccharide core and a slightly variant lipid A. The diversity and differences in the structure of the Y. pestis LPS suggest important contributions of these variations to the pathogenesis of this organism, potentially related to innate and acquired immune recognition of Y. pestis and epidemiologic means to detect, classify, control and respond to Y. pestis infections.
Knirel
Yuriy A
YA
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia.
Lindner
Buko
B
Vinogradov
Evgeny V
EV
Kocharova
Nina A
NA
Senchenkova
Sof'ya N
SN
Shaikhutdinova
Rima Z
RZ
Dentovskaya
Svetlana V
SV
Fursova
Nadezhda K
NK
Bakhteeva
Irina V
IV
Titareva
Galina M
GM
Balakhonov
Sergey V
SV
Holst
Otto
O
Gremyakova
Tat'yana A
TA
Pier
Gerald B
GB
Anisimov
Andrey P
AP
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D015010:Yersinia pestis / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development
D008070:Lipopolysaccharides; D009844:Oligosaccharides