title: Global gene expression profile of Yersinia pestis induced by streptomycin.
abstract: Plague, caused by Yersinia pestis, is one of the most dangerous diseases that impressed a horror onto human consciousness that persists to this day. Cases of plague can be normally controlled by timely antibiotic administration. Streptomycin is the first-line antibiotic for plague treatment. In this study, a DNA microarray was used to investigate the changes in the gene expression profile of Y. pestis upon exposure to streptomycin. A total of 345 genes were identified to be differentially regulated, 144 of which were up-regulated, and 201 down-regulated. Streptomycin-induced transcriptional changes occurred in genes responsible for heat shock response, drug/analogue sensitivity, biosynthesis of the branched-chain amino acids, chemotaxis and mobility and broad regulatory functions. A wide set of genes involved in energy metabolism, biosynthesis of small macromolecules, synthesis and modification of macromolecules and degradation of small and macro molecules were among those down-regulated. The results reveal general changes in gene expression that are consistent with known mechanisms of action of streptomycin and many new genes that are likely to play important roles in the response to streptomycin treatment, providing useful candidates for investigating the specific mechanisms of streptomycin action.
authors.affiliation: Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
authors.forename: Jingfu
authors.initials: J
authors.lastname: Qiu
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Zongzhong
authors.initials: Z
authors.lastname: Tong
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Erhei
authors.initials: E
authors.lastname: Dai
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhai
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013307:Streptomycin / Q000494:pharmacology*; D014158:Transcription, Genetic; D015010:Yersinia pestis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D013307:Streptomycin