title: Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification.
abstract: A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea.
authors.forename: Hyeon-Su
authors.initials: HS
authors.lastname: Ro
authors.forename: Sun Ok
authors.initials: SO
authors.lastname: Jung
authors.forename: Byung Hoon
authors.initials: BH
authors.lastname: Kho
authors.forename: Hyung Pyo
authors.initials: HP
authors.lastname: Hong
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Lee
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Shin
authors.forename: Min Gon
authors.initials: MG
authors.lastname: Kim
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D004950:Esterases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; D006639:Histidine; D004950:Esterases