title: Interactions of multiple heparin binding growth factors with neuropilin-1 and potentiation of the activity of fibroblast growth factor-2.
abstract: The hypothesis that neuropilin-1 (Npn-1) may interact with heparin-binding proteins other than vascular endothelial growth factor has been tested using an optical biosensor-based binding assay. The results show that fibroblast growth factor (FGF) 1, 2, 4, and 7, FGF receptor 1, hepatocyte growth factor/scatter factor (HGF/SF), FGF-binding protein, normal protease sensitive form of prion protein, antithrombin III, and Npn-1 itself are all able to interact with Npn-1 immobilized on the sensor surface. FGF-2, FGF-4, and HGF/SF are also shown to interact with Npn-1 in a solution assay. Moreover, these protein-protein interactions are dependent on the ionic strength of the medium and are inhibited by heparin, and the kinetics of binding of FGF-2, FGF-4 and HGF/SF to Npn-1 are characterized by fast association rate constants (270,000-1,600,000 m(-1) s(-1)). These results suggest that Npn-1 possesses a "heparin" mimetic site that is able to interact at least in part through ionic bonding with the heparin binding site on many of the proteins studied. Npn-1 was also found to potentiate the growth stimulatory activity of FGF-2 on human umbilical vein endothelial cells, indicating that Npn-1 may not just bind but also regulate the activity of heparin-binding proteins.
authors.affiliation: School of Biological Sciences, Biosciences Building, University of Liverpool, Liverpool, L69 7ZB, United Kingdom.
authors.forename: David C
authors.initials: DC
authors.lastname: West
authors.forename: Chris G
authors.initials: CG
authors.lastname: Rees
authors.forename: Laurence
authors.initials: L
authors.lastname: Duchesne
authors.forename: Susannah J
authors.initials: SJ
authors.lastname: Patey
authors.forename: Carla J
authors.initials: CJ
authors.lastname: Terry
authors.forename: Jeremy E
authors.initials: JE
authors.lastname: Turnbull
authors.forename: Maryse
authors.initials: M
authors.lastname: Delehedde
authors.forename: Christian W
authors.initials: CW
authors.lastname: Heegaard
authors.forename: Fabrice
authors.initials: F
authors.lastname: Allain
authors.forename: Christophe
authors.initials: C
authors.lastname: Vanpouille
authors.forename: Dina
authors.initials: D
authors.lastname: Ron
authors.forename: David G
authors.initials: DG
authors.lastname: Fernig
mesh_terms: D000818:Animals; D001665:Binding Sites; D015374:Biosensing Techniques; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D039942:Neuropilin-1 / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D017434:Protein Structure, Tertiary; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D020033:Protein Isoforms; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D039942:Neuropilin-1; D017228:Hepatocyte Growth Factor; D006493:Heparin; C496348:FGFR1 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1