title: [Simultaneous determination of amino acids in food by capillary electrophoresis with indirect ultraviolet detection].
abstract: The characteristic of capillary electrophoresis (CE) with indirect ultraviolet (UV) detection of L-ornithine (Orn), L-proline (Pro) and L-glutamine (Gln) was investigated. A suitable electrolyte consists of 5 mmol/L sodium p-aminobenzene sulfonic acid-10 mmol/L KH2PO4 (pH 11.5) with UV detection at 254 nm. The applied voltage was set at 12 kV. The three amino acids were completely separated and detected by the method in 11 min. The relative standard deviations (RSD) of the method were better than 0.72% for migration time and 2.0% for peak height. The detection limits of Orn, Pro and Gln were 6.78, 8.71 and 7.86 mg/L, respectively. The proposed method was successfully applied to the determination of amino acids in foods (Haitian soybean sauce, Tsingtao beer and amino acids tonic) and the recoveries were in the range of 96.8% - 104%.
authors.affiliation: Nanjing University of Technology, Nanjing 210009, China. zjlyxing@yahoo.com.cn
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Xiang
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001515:Beer / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D005973:Glutamine / Q000032:analysis; D009952:Ornithine / Q000032:analysis; D011392:Proline / Q000032:analysis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D000596:Amino Acids; D005973:Glutamine; D011392:Proline; D009952:Ornithine