Overexpression of Nd1-s, a variant form of new kelch family protein, perturbs the cell cycle progression of fibroblasts.
The murine Nd1 gene encodes two forms of protein, Nd1-L and Nd1-S, both of which share the BTB/POZ domain, but Nd1-S lacks the kelch repeats. Although Nd1-L ubiquitously expresses, localizes in the cytoplasm and functions as a stabilizer of actin filaments, expression and function of Nd1-S were unknown. Here we show that Nd1-S were expressed in all tissues examined and localized in the nucleus as a speckled-like pattern. Furthermore, overexpression of Nd1-S perturbed cell growth of NIH3T3 cells at the G1/S phase of the cell cycle. These results suggest that Nd1-S may play a role in cell cycle progression in the nucleus.
Inoue
Atsushi
A
Department of Developmental Genetics (H2), Graduate School of Medicine, Chiba University, Chiba, Japan.
Kang
Myengmo
M
Fujimura
Lisa
L
Takamori
Yasuyuki
Y
Sasagawa
Kazushi
K
Itoh
Haruo
H
Tokuhisa
Takeshi
T
Hatano
Masahiko
M
D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000737:chemistry; D049109:Cell Proliferation; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D041681:NIH 3T3 Cells; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection
D047908:Intracellular Signaling Peptides and Proteins; C432129:Nd1 protein, mouse; D011506:Proteins; D012333:RNA, Messenger