title: Use of the restriction enzyme EcoRI for pulsed-field gel electrophoretic analysis of Helicobacter pylori.
abstract: Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (typeability was 97%).
authors.affiliation: Department of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa 228, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hosaka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Irinoda
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nakano
authors.forename: Satosi
authors.initials: S
authors.lastname: Tanabe
authors.forename: Wasaburo
authors.initials: W
authors.lastname: Koizumi
authors.forename: Katunori
authors.initials: K
authors.lastname: Saigenji
authors.forename: Matsuhisa
authors.initials: M
authors.lastname: Inoue
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D016521:Electrophoresis, Gel, Pulsed-Field; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI