Digging deeper into the plant cell wall proteome.
The proteome of the plant cell wall/apoplast is less well characterized than those of other subcellular compartments. This largely reflects the many technical challenges involved in extracting and identifying extracellular proteins, many of which resist isolation and identification, and in capturing a population that is both comprehensive and relatively uncontaminated with intracellular proteins. However, a range of disruptive techniques, involving tissue homogenization and subsequent sequential extraction and non-disruptive approaches has been developed. These approaches have been complemented more recently by other genome-scale screens, such as secretion traps that reveal the genes encoding proteins with N-terminal signal peptides that are targeted to the secretory pathway, many of which are subsequently localized in the wall. While the size and complexity of the wall proteome is still unresolved, the combination of experimental tools and computational prediction is rapidly expanding the catalog of known wall-localized proteins, suggesting the unexpected extracellular localization of other polypeptides and providing the basis for further exploration of plant wall structure and function.
Lee
Sang-Jik
SJ
Department of Plant Biology, 228 Plant Science Building, Cornell University, Ithaca, NY 14853, USA.
Saravanan
Ramu S
RS
Damasceno
Cynthia M B
CM
Yamane
Hisayo
H
Kim
Byung-Dong
BD
Rose
Jocelyn K C
JK
D002473:Cell Wall / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000235:genetics
D010940:Plant Proteins; D020543:Proteome