title: A clinicopathological study of the expression of extracellular matrix components in urothelial carcinoma.
abstract: OBJECTIVE
  To measure the immunohistochemical expression of the extracellular matrix (ECM) components tenascin, fibronectin, collagen type IV and laminin in urothelial carcinomas, and to correlate their expression with clinicopathological features to clarify the prognostic value of these molecules and their role in tumour progression.
  
  
  METHODS
  Tumour specimens obtained during transurethral resection of bladder tumour (TURBT) from 103 patients (82 men and 2 1 women, mean age 66.7 years, range 27-89) were studied retrospectively. The expression of tenascin, fibronectin, collagen type IV and laminin was correlated with clinicopathological features (tumour grade and stage, multiplicity, simultaneous in situ component, the proliferative activity as estimated by the two proliferation associated indices, Ki-67 and proliferating cell nuclear antigen, the recurrence rate, and the progression of invading tumour). Specimens investigated for tenascin expression from patients with superficial bladder cancers were categorized into 28 treated by TURBT only and 53 who had TURBT followed by intravesical instillations of interferon.
  
  
  RESULTS
  Cytoplasmic tenascin expression was detected in tumour cells in 20% of specimens. Tenascin was expressed in the tumour stroma in 76% of specimens, and was positively correlated with tumour grade and stage. Stromal tenascin expression was positively correlated with proliferative activity, and with the expression of fibronectin and collagen type IV. Fibronectin was expressed in the tumour stroma in 89% of specimens and was positively correlated with tumour stage, proliferative activity, and expression of collagen type IV and laminin. Collagen type IV was expressed in 93% of specimens, and was positively correlated with tumour grade and stage. Laminin was expressed in 78% of specimens and had no significant correlation with the clinicopathological features. Patients treated with TURBT alone and who had low levels of tenascin had a longer tumour-free interval than those with high levels of tenascin.
  
  
  CONCLUSIONS
  Levels of tenascin might be valuable for predicting the risk of early recurrence. The expression of tenascin, fibronectin and collagen type IV seems to be correlated with more aggressive tumour behaviour. Furthermore, their interrelationships could indicate that they are involved in the remodelling of bladder cancer tissue, probably influencing tumour progression.
authors.affiliation: Department of Pathology, Medical School, University of Ioannina, Greece.
authors.forename: Elli
authors.initials: E
authors.lastname: Ioachim
authors.forename: Michalis
authors.initials: M
authors.lastname: Michael
authors.forename: Nicolaos E
authors.initials: NE
authors.lastname: Stavropoulos
authors.forename: Evangelia
authors.initials: E
authors.lastname: Kitsiou
authors.forename: Marios
authors.initials: M
authors.lastname: Salmas
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Malamou-Mitsi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D024141:Collagen Type IV / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D012189:Retrospective Studies; D019063:Tenascin / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D024141:Collagen Type IV; D005353:Fibronectins; D007797:Laminin; D019063:Tenascin