title: Expression of c-kit messenger ribonucleic acid and c-kit protein in sigmoid colon of patients with slow transit constipation.
abstract: OBJECTIVE
  The c-kit protooncogene receptor and its ligand stem cell factor regulate the proliferation and survival of germ cells as well as interstitial cells of Cajal (ICCs). Decreased numbers of ICCs and defects in its networks have been reported in the colon of patients with slow transit constipation (STC). However, little information about the c-kit messenger ribonucleic acid (mRNA) and protein expression in the constipated colon is available. The aim of this study was to determine whether the expression of c-kit mRNA and c-kit protein declined in the colon in STC.
  
  
  METHODS
  The sigmoid colonic samples from 12 patients with STC and from eight age-matched patients with non-obstructed colorectal cancer were used for this study. Expression of c-kit mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR), and expression of c-kit protein was detected by Western blot analysis.
  
  
  RESULTS
  Decreased expression of c-kit mRNA was demonstrated in the STC group compared with the control group. The ratio of c-kit and beta-actin was 1.26+/-0.32 in controls and 1.17+/-0.41 in the STC group (U=0.500, P=0.029). c-kit protein expression significantly declined in the STC group. The mean value of optical density was 162.97+/-5.43 in the control group and 96.64+/-8.80 in the STC group (U=0.000, P=0.021).
  
  
  CONCLUSIONS
  The data indicate that the expression of c-kit mRNA and c-kit protein significantly decreased in the colon of STC, suggesting that the c-kit signal pathway may play an important role in ICC reduction in STC.
authors.affiliation: Department of General Surgery, Surgery Research Institute, Daping Hospital, Third Military Medical University, Chongqing 400042, China. vd_tong@yahoo.com.cn
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tong
authors.forename: Bao-Hua
authors.initials: BH
authors.lastname: Liu
authors.forename: Lian-Yang
authors.initials: LY
authors.lastname: Zhang
authors.forename: Ren-Ping
authors.initials: RP
authors.lastname: Xiong
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Sheng-Ben
authors.initials: SB
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D016022:Case-Control Studies; D003248:Constipation / Q000209:etiology* / Q000235:genetics*; D005260:Female; D005769:Gastrointestinal Motility / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D012333:RNA, Messenger; D019009:Proto-Oncogene Proteins c-kit