Template DNA ratio can affect detection by genus-specific PCR-denaturing gradient gel electrophoresis of bacteria present at low abundance in mixed populations.
The detection of Helicobacter species by genus-specific polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was compared with that by species-specific PCR in murine intestinal samples. Results suggest that, in samples containing multiple Helicobacter species, genus-specific PCR-DGGE may fail to detect all Helicobacter species present and that this relates to the initial template DNA ratio.
Zhang
Li
L
The School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia.
Danon
Stephen
S
Grehan
Martin
M
Lee
Adrian
A
Mitchell
Hazel
H
D000818:Animals; D001431:Bacteriological Techniques; D002432:Cecum / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D020459:Genes, rRNA; D016998:Helicobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000382:microbiology; D051379:Mice; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013698:Templates, Genetic
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S