[Determination of scopoletin and umbelliferone contents in Saussurea medusa Maxim by high-performance liquid chromatography].
The contents of scopoletin and umbelliferone in Saussurea medusa Maxim were determined by high-performance liquid chromatography (HPLC) using Merck Lichrospher100 RP-18e column (250 mm x 4.0 mm, 5 microm) with the mobile phase of methanol-tetrahydrofuran-water solution and the absorbance of the compounds observed at 346 nm. HPLC yielded an average recovery and RSD of 100.07% and 1.42% for scopoletin, and 99.41% and 2.06% for umbelliferone, respectively, suggesting that HPLC is a simple, rapid and accurate method for quality control of Saussurea medusa Maxim.
Su
Wei-wei
WW
Postgraduate Division, Department of Pharmaceutics, Southern Medical University, Guangzhou 510515, China.
Zhao
Jie
J
Lin
Jing-ming
JM
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D030441:Saussurea / Q000737:chemistry*; D012603:Scopoletin / Q000032:analysis*; D014468:Umbelliferones / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D014468:Umbelliferones; C031477:7-hydroxycoumarin; D012603:Scopoletin