title: Persistence of Escherichia coli O157 isolates on bovine farms in England and Wales.
abstract: We performed pulsed-field gel electrophoresis on Escherichia coli O157 isolates (n = 318) from 199 healthy animals in a longitudinal study carried out on nine farms. Investigation of the restriction types proved that at the farm level, the same clones can be detected on sampling occasions separated by as much as 17 months. The cohort animals were repeatedly sampled, and for some of these, the same clones were obtained on sampling occasions separated by as much as 8 months.
authors.affiliation: Department of Food and Environment Safety, Veterinary Laboratories Agency--Weybridge, Addlestone, Surrey UK. E.liebana@VLA.DEFRA.gsi.gov.UK
authors.forename: Ernesto
authors.initials: E
authors.lastname: Liebana
authors.forename: Richard P
authors.initials: RP
authors.lastname: Smith
authors.forename: Miranda
authors.initials: M
authors.lastname: Batchelor
authors.forename: Ian
authors.initials: I
authors.lastname: McLaren
authors.forename: Claire
authors.initials: C
authors.lastname: Cassar
authors.forename: Felicity A
authors.initials: FA
authors.lastname: Clifton-Hadley
authors.forename: Giles A
authors.initials: GA
authors.lastname: Paiba
mesh_terms: D000383:Agriculture; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004197:Disease Reservoirs; D016521:Electrophoresis, Gel, Pulsed-Field; D004739:England; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008137:Longitudinal Studies; D014852:Wales
chemical_list: D004269:DNA, Bacterial