title: Rapid and simple quantification of bacterial cells by using a microfluidic device.
abstract: This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Sakamoto
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Masao
authors.initials: M
authors.lastname: Nasu
mesh_terms: D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation*; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D016958:Pseudomonas putida / Q000302:isolation & purification*; D045483:Rivers / Q000382:microbiology; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D003470:Culture Media; D009930:Organic Chemicals; C098798:SYBR Green II