[Optimized RT-PCR based detection of specific genetic abnormalities within malignant hematopoietic disorders].
The moleculargenetic detection of specific genetic abnormalities within malignant hematopoietic disorders is an important diagnostic tool with relevance for the differential diagnosis, therapy and prognosis.According to a modified and optimized RT-PCR based technique native bone marrow aspirates and peripheral blood samples of 183 patients were investigated for the presence of specific genetic abnormalities. The histological diagnosis of all CMLs t(9;22) and variants of AMLs M3 t(15;17) and t(11;17) were moleculargenetically confirmed. Additionally one t(5;12) positive chronic myelomonozytic leukemia, one t(8;21) positive AML M1, one t(9;22) positive B-ALL and in each case one t(6;9) and one t(3;21) positive myelodysplastic syndrome were detected. The hereby described method is a simple, specific and reliable technique for the rapid moleculargenetic detection of specific genetic abnormalities within malignant hematopoietic disorders with implication for the diagnosis/differential diagnosis, prognosis and therapy.
Gattenlöhner
S
S
Institut für Pathologie, Universität Würzburg, Germany. stefan.gattenloehner@mail.uni-wuerzburg.de
Bonengel
M
M
Müller-Hermelink
H K
HK
D015972:Gene Expression Regulation, Neoplastic; D030342:Genetic Diseases, Inborn / Q000235:genetics* / Q000473:pathology; D019337:Hematologic Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction