title: Anti-CENP-H antibodies in patients with Sjogren's syndrome.
abstract: Anti-centromere antibody (ACA) has been reported to be associated with Sjogren's syndrome (SS) and the clinical significance of anti-CENP-H antibody remains unknown. To determine the clinical significance of anti-CENP-H and anti-centromere antibodies in primary SS, sera from 62 patients with primary SS and 40 normal controls were examined for anti-SS-A/SS-B antibodies, ACA and anti-CENP-H antibodies, by enzyme-linked immunosorbent assay and indirect immunofluorescence (IIF), respectively. Of the 62 serum samples with primary SS, 17 were positive with ACA and anti-CENP-H antibodies. Sera from SS patients with anti-CENP-H and ACA antibodies do not contain anti-SS-A/Ro and/or anti-SS-B/La antibodies. No anti-CENP-H antibody was found in sera of normal controls. An increased frequency of ACA and anti-CENP-H antibodies was found for the first time in patients with SS. Anti-CENP-H antibodies and anti-SS-A/Ro or anti-SS-B/La antibodies are present mutually exclusive. Patients with anti-CENP-H antibodies had a lower frequency of rheumatoid factor (RF). SS can be subdivided serologically into two groups; group one with anti-SS-A/Ro and/or anti-SS-B/La antibody, group two with ACA and/or anti-CENP-H antibodies. We recommend that ACA or anti-CENP-H antibodies should be considered as one of the serological markers for SS.
authors.affiliation: Department of Medicine and Institute of Immunology, Chung Shan Medical University, 110 Sec.1, Chien Kuo N. Road, Taichung, 402, Taiwan.
authors.forename: Tsai-Ching
authors.initials: TC
authors.lastname: Hsu
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Chang
authors.forename: Ming-Cheng
authors.initials: MC
authors.lastname: Lin
authors.forename: Song-Tao
authors.initials: ST
authors.lastname: Liu
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Yen
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Tsay
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood*; D001324:Autoantigens / Q000096:biosynthesis / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology / Q000473:pathology; D002503:Centromere / Q000276:immunology*; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007124:Immunoenzyme Techniques; D018668:Muntjacs; D011817:Rabbits; D012217:Rheumatoid Factor / Q000097:blood; D012859:Sjogren's Syndrome / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; C121933:CENPH protein, human; D002868:Chromosomal Proteins, Non-Histone; C035356:SS-A antibodies; C035357:SS-B antibodies; D012217:Rheumatoid Factor