[The preparation of chemical-bonded hyperbranched polymer coated column for capillary electrophoresis and their application to separation of basic proteins].
A series of hyperbranched poly(amine-ester)s were synthesized using one-step and pseudo-step procedure respectively, and their molecular structures were characterized by IR, hydroxyl value and viscosity. These polymers were coated on the inner surface of the fused-silica capillaries in a chemical bonding process. The electroosmotic flow and the separation capability for basic proteins of the coated capillaries were investigated. The results demonstrated that the coating in the capillaries could greatly reduce the electroosmotic flow and effectively suppress protein adsorption in the pH range of 3-7. The separation efficiency of the coated capillary of hyperbranched poly(amine-ester)s with trimethylolpropane and pentaerythritol as the core was 10(5)/m and 10(7)/m, respectively. The separation efficiency and the stability of basic proteins on these coated capillaries were found excellent.
Shou
Chongqi
C
Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China. shoucq@sina.com
Zhang
Zhiliang
Z
Zhou
Changli
C
Zhao
Chunbin
C
Li
Guanbin
G
Chen
Liren
L
D000327:Adsorption; D053841:Electroosmosis / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014783:Viscosity
D011108:Polymers; D011409:Propylene Glycols; D011506:Proteins; C018163:trimethylolpropane; D012822:Silicon Dioxide; C008783:pentaerythritol