SOS-independent induction of dinB transcription by beta-lactam-mediated inhibition of cell wall synthesis in Escherichia coli.
Transcription of the dinB gene, encoding DNA polymerase IV, is induced by the inhibition of cell wall synthesis at different levels. Using the beta-lactam antibiotic ceftazidime, a PBP3 inhibitor, as a model, we have shown that this induction is independent of the LexA/RecA regulatory system. Induction of dinB transcription mediated by ceftazidime produces an increase in the reversion of a +1 Lac frameshift mutation.
Pérez-Capilla
Tatiana
T
Departamento de Biotecnología Microbiana, Centro Nacional de Biotechnología, Universidad Autónoma de Madrid, Spain.
Baquero
María-Rosario
MR
Gómez-Gómez
José-María
JM
Ionel
Alina
A
Martín
Soledad
S
Blázquez
Jesús
J
D000251:Adenosine Triphosphatases / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology; D002442:Ceftazidime / Q000494:pharmacology*; D002473:Cell Wall / Q000378:metabolism*; D004265:DNA Helicases / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D016368:Frameshift Mutation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D013014:SOS Response, Genetics; D012697:Serine Endopeptidases / Q000502:physiology; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics; D000099160:LexA Repressor Protein
D000251:Adenosine Triphosphatases; D001426:Bacterial Proteins; D002442:Ceftazidime; D004265:DNA Helicases; D004791:Enzyme Inhibitors; D029968:Escherichia coli Proteins; D012697:Serine Endopeptidases; D001616:beta-Galactosidase; D000099160:LexA Repressor Protein; C094399:DinB protein, E coli