title: Introduction of tyramide signal amplification (TSA) to pre-embedding nanogold-silver staining at the electron microscopic level.
abstract: The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.
authors.affiliation: Department of Anatomy, Chonnam National University Medical School, Gwangju, Korea. seunglee@chonnam.ac.kr
authors.forename: Seung-won
authors.initials: SW
authors.lastname: Lee
authors.forename: Song Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Seong Hyuk
authors.initials: SH
authors.lastname: Ko
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Hong
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Nam
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Shuhei
authors.initials: S
authors.lastname: Imayama
authors.forename: Yeong-Joon
authors.initials: YJ
authors.lastname: Park
authors.forename: Kyu Youn
authors.initials: KY
authors.lastname: Ahn
authors.forename: Choon Sang
authors.initials: CS
authors.lastname: Bae
authors.forename: Baik Yoon
authors.initials: BY
authors.lastname: Kim
authors.forename: Sung Sik
authors.initials: SS
authors.lastname: Park
mesh_terms: D001324:Autoantigens; D045744:Cell Line, Tumor; D006046:Gold; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008545:Melanoma; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D016622:Silver Staining; D014439:Tyramine
chemical_list: D001324:Autoantigens; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; D006046:Gold; D014439:Tyramine