title: Human parechovirus 3 and neonatal infections.
abstract: A third serotype of human parechovirus (HPeV) has been recently isolated from stool specimens of a young Japanese child with transient paralysis. We report 3 additional cases of neonatal sepsis caused by HPeV-3 in the fall of 2001 in Canadian infants 7-27 days old. All children were hospitalized with high fever, erythematous rash, and tachypnea for a median of 5 days. The viruses isolated from nasopharyngeal aspirates grew slowly on tertiary monkey kidney cells and were successfully passaged on Vero cells. The predicted amino acid identity of the VP0-VP3-VP1 region of the three viruses was 74.6%-74.8%, 73.4%-73.6%, and 97.0%-97.1% when compared to HPeV-1, -2, and -3 prototype strains, respectively. Although different, our isolates were closely related; amino acid identity was 99.6%-100% for the last 3 proteins.
authors.affiliation: Centre Hospitalier Universitaire de Québec and Laval University, Québec City, Québec, Canada. Guy.Boivin@crchul.ulaval.ca
authors.forename: Guy
authors.initials: G
authors.lastname: Boivin
authors.forename: Yacine
authors.initials: Y
authors.lastname: Abed
authors.forename: François D
authors.initials: FD
authors.lastname: Boucher
mesh_terms: D001483:Base Sequence; D002170:Canada; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D030061:Parechovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000175:diagnosis / Q000821:virology*; D018805:Sepsis / Q000175:diagnosis / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral