[Study on optimizing extraction process of root of Polygonum multiflorum].
OBJECTIVE
To optimize the extraction process of root of Polygoni multflori and validate the interrelation between antioxdative capacity and the content of 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside in root of P. multiflori.


METHODS
The optimum extraction was abserved with the orthogonal design; 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside was determined by HPLC and the antioxidative capacity by using photochemiluminescence detection method with the Photochem supplied by Analytik Jena AG; the concentration of ethanol, amount of ethanol, extraction time and extraction times were the four factors in the extraction.


RESULTS
The concentration of ethanol and extraction times had significant effect on the content of 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside and the antioxidative capacity of the crude extract.


CONCLUSIONS
The best extraction process is to extract three times by using 10 fold EtOH(60%), refluxing at 85 degrees C and to extract one and half hours each time.
Bai
Hai-bo
HB
Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
Wang
Jian-fei
JF
Long
Juan
J
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology; D000431:Ethanol; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D013267:Stilbenes / Q000032:analysis / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013267:Stilbenes; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; D000431:Ethanol