title: MHC RFLP analyses in channel catfish full-sibling families: identification of the role of MHC molecules in spontaneous allogeneic cytotoxic responses.
abstract: Genes encoding MHC class I and II molecules have been identified in a number of fish species, including the channel catfish, but there is still a dearth of knowledge concerning their functional roles in teleost immune responses. This has in part been due to a lack of appropriate MHC class I and II matched and mismatched animals. To identify such animals, MHC segregation and linkage studies in the channel catfish were undertaken. The results of restriction fragment length polymorphism and fluorescent in situ hybridization studies showed that all the MHC class II genes are linked and most if not all MHC class I genes are linked. These studies also demonstrated that in catfish, as in other teleosts, MHC class I and II genes are not linked. Consequently, catfish matched and mismatched for MHC class I and II genes were identified and preliminary functional studies indicate that spontaneous non-specific allogeneic cytotoxic responses are likely mediated by differences in MHC class I, but not class II, region molecules.
authors.affiliation: Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA. squiniou@ars.usda.gov
authors.forename: Sylvie M-A
authors.initials: SM
authors.lastname: Quiniou
authors.forename: Melanie
authors.initials: M
authors.lastname: Wilson
authors.forename: Eva
authors.initials: E
authors.lastname: Bengtén
authors.forename: Geoffrey C
authors.initials: GC
authors.lastname: Waldbieser
authors.forename: L William
authors.initials: LW
authors.lastname: Clem
authors.forename: Norman W
authors.initials: NW
authors.lastname: Miller
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D008040:Genetic Linkage; D006649:Histocompatibility Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007059:Ictaluridae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000502:physiology*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II