title: Biochemical and functional evidence for heteromeric assembly of P2X1 and P2X4 subunits.
abstract: P2X receptors are ligand-gated ion channels activated by extracellular ATP. In expression systems, P2X subunits form homo- and heterotrimeric receptors. Heteromerization is also likely to occur in vivo as (i) most P2X subunits show overlapping distribution in different tissues and (ii) the functional properties of many native P2X receptors differ from those of heterologously expressed homomeric receptors. Here, we used the Xenopus laevis oocyte expression system to test for heteromerization of P2X1 and P2X4 subunits. Upon co-injection, P2X4 subunits were co-purified with hexahistidyl-tagged P2X1 subunits indicating heteromerization. Blue native polyacrylamide gel electrophoresis (BN-PAGE) analysis of these P2X complexes excluded artificial aggregation and confirmed that both subunits were present in trimeric complexes of the same size. Two-electrode voltage-clamp experiments revealed functional P2X receptors with kinetic properties resembling homomeric P2X4 receptors and a pharmacological profile similar to homomeric P2X1 receptors. Thus, application of alpha,beta-methylene ATP evoked a slowly desensitizing current sensitive to the antagonists suramin and 2',3'-O-(2,4,6-trinitrophenyl)-ATP. This study provides for the first time biochemical and functional evidence for the formation of heteromeric P2X(1+4) receptors. These receptors may account for native P2X mediated responses that until now could not be correlated with previously described recombinant P2X receptors.
authors.affiliation: Department of Molecular Biology of Neuronal Signals, Max-Planck Institute for Experimental Medicine, Göttingen, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Nicke
authors.forename: Daniel
authors.initials: D
authors.lastname: Kerschensteiner
authors.forename: Florentina
authors.initials: F
authors.lastname: Soto
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D005260:Female; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D058910:Purinergic P2 Receptor Agonists; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D058469:Receptors, Purinergic P2X; D058848:Receptors, Purinergic P2X4; D013498:Suramin / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D021122:Protein Subunits; D058910:Purinergic P2 Receptor Agonists; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D058848:Receptors, Purinergic P2X4; D013498:Suramin; D000255:Adenosine Triphosphate