Cyclic nucleotide signaling mediates an odorant-suppressible chloride conductance in lobster olfactory receptor neurons.
We have previously shown that lobster olfactory receptor neurons (ORNs) express an odorant-suppressible Cl(-) conductance that modulates the output of the cells. Here, we develop a more complete pharmacological profile of this conductance, showing it is blockable by the Cl(-) channel blockers DIDS, 9-AC and flufenamic acid, but not by niflumic acid. We then show that a conductance with this pharmacological profile is mediated by cyclic nucleotide signaling. These findings further our understanding of the cellular mechanisms through which odorants can modulate the output of lobster ORNs.
Doolin
Richard E
RE
Whitney Laboratory, University of Florida, 9505 Ocean Shore Blvd., St. Augustine, FL 32080, USA.
Ache
Barry W
BW
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000502:physiology; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004558:Electric Stimulation / Q000379:methods; D005439:Flufenamic Acid / Q000494:pharmacology; D007097:Imines / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000037:antagonists & inhibitors / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000502:physiology*; D033521:Palinuridae / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
D000067956:Adenylyl Cyclase Inhibitors; D000873:Anthracenes; D018118:Chloride Channels; D007097:Imines; D009712:Nucleotides, Cyclic; D005576:Colforsin; D005439:Flufenamic Acid; C010030:9-anthroic acid; C014925:RMI 12330A; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000225:Adenine; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D015056:1-Methyl-3-isobutylxanthine