Antioxidant parameters and lipid peroxidation in salivary glands of streptozotocin-induced diabetic rats.
BACKGROUND
There is evidence suggesting an unbalance between oxidant and antioxidant status associated with diabetes. Considering that salivary function is essential for the maintenance of oral and systemic health, this study was designed to examine the levels of reduced and oxidized glutathione and the activities of the antioxidant enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase, in salivary gland of streptozotocin-induced diabetic rats.


METHODS
The content of malondialdehyde was determined in the blood and in the salivary glands. The antioxidant status was investigated in the submandibular and parotid salivary glands.


RESULTS
Diabetic rats showed an increase in the content of malonaldehyde in the blood and in the submandibular salivary gland, but not in the parotid gland. Both forms, reduced and oxidized glutathione content present higher values in the diabetic submandibular gland compared with controls. No difference in the activity of superoxide dismutase between the diabetic and control glands was observed in either gland. Catalase showed higher specific activity in the parotid gland of the diabetic rats than control; however, in the submandibular gland, only when expressed as unit per gland was it higher than control. The specific activity of glutathione peroxidase was higher in the diabetic parotid gland than control; however, in the submandibular gland, its activity per gland was lower than controls.


CONCLUSIONS
The streptozotocin-induced diabetes in rats caused different results comparing the submandibular and parotid salivary glands.
Nogueira
Fernando N
FN
Oral Biology Research Center, Faculty of Dentistry, University of São Paulo, Av. Prof Lineu Prestes, 2227 CEP 05508-000 São Paulo, Brasil.
Carvalho
Adriana M
AM
Yamaguti
Paula M
PM
Nicolau
José
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000378:metabolism*; D013311:Streptozocin
D000975:Antioxidants; D013311:Streptozocin