An aqueous Chlorella extract inhibits IL-5 production by mast cells in vitro and reduces ovalbumin-induced eosinophil infiltration in the airway in mice in vivo.
An aqueous extract of the edible microalga (CP) (1), has recently been tested for its immunomodulatory effects in a human clinical trial. Here, the CP extract was dialyzed and fractionated using Sephadex G 100 chromatography. The effects of a dialyzed aqueous CP extract, fraction 2 , on mast cell mediator release in vitro and ovalbumin-induced allergic airway inflammation in vivo were examined. In vitro, treatment of mouse bone marrow-derived mast cells with 2 for 18 h significantly inhibited antigen (trinitrophenyl-BSA)-induced IL-5 production. In vivo, treatment of mice with 2 during ovalbumin sensitization and stimulation process significantly reduced eosinophil and neutrophil infiltration in the airways. Moreover, fractions obtained by size exclusion chromatography of 2 inhibited IgE-dependent cytokine GM-CSF production from human cord blood-derived mast cells. Taken together, these results suggest that 2 is composed of biopolymers with anti-allergic potential.
Kralovec
J A
JA
Ocean Nutrition Canada Ltd., Bioscience Enterprise Centre, 1721 Lower Water Street, Halifax, NS, Canada B3J 1S5. jkralovec@ocean-nutrition.com
Power
M R
MR
Liu
F
F
Maydanski
E
E
Ewart
H S
HS
Watson
L V
LV
Barrow
C J
CJ
Lin
T J
TJ
D000818:Animals; D002478:Cells, Cultured; D002708:Chlorella / Q000737:chemistry*; D019587:Dietary Supplements; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D007073:Immunoglobulin E / Q000276:immunology; D015848:Interleukin-5 / Q000096:biosynthesis*; D008168:Lung / Q000187:drug effects* / Q000276:immunology*; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010047:Ovalbumin
D015848:Interleukin-5; D007073:Immunoglobulin E; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010047:Ovalbumin