title: Control of heme peptide activity by using phase transition polymers modified with inhibitors.
abstract: Catalytic activity of a heme peptide (HP) modified-electrode for H(2)O(2) reduction was controlled by use of poly(N-isopropylacrylamide) modified with an inhibitory moiety, imidazole group. The polymers inhibited the catalytic activity below their lower critical solution temperature (LCST) where the polymers were dissolved and did not inhibit the activity above the LCST where the polymers were precipitated. A polymer with a longer side chain connecting with the imidazole group was more inhibitory than a polymer with a shorter side chain at temperatures below the LCST. Formation constants of dissolved HP-imidazole complexes were evaluated by spectroscopic means, and it was found that the polymers were more inhibitory than the corresponding monomers.
authors.affiliation: Institute of Industrial Science, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8505, Japan.
authors.forename: Kikuo
authors.initials: K
authors.lastname: Komori
authors.forename: Hitomi
authors.initials: H
authors.lastname: Matsui
authors.forename: Tetsu
authors.initials: T
authors.lastname: Tatsuma
mesh_terms: D000180:Acrylic Resins; D002384:Catalysis; D004566:Electrodes; D006420:Hemeproteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D010084:Oxidation-Reduction; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D006420:Hemeproteins; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D006861:Hydrogen Peroxide