Purification of L-lysine in simulated moving bed and fixed-bed chromatography.
L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed (SMB) chromatography. The separation conditions including pH, eluent concentration and Lys+ and Lys2+ adsorption isotherms were studied in batch adsorption. The column capacity, eluent flow rate and eluent concentration have been studied in fixed-bed chromatography. Maximum purification rate of lysine was obtained as 0.066 g/(g x h) (per gram resin and per hour) at an eluent flow rate of 10 mL/min in fixed-bed chromatography. The results obtained from SMB were 0.11 g/(g x h) for L-lysine purification rate and 96% for L-lysine recovery.
Robatjazi
Seyed Mortaza
SM
Biotechnology Group, Department of Chemical Engineering, Tarbiat Modarres University, Tehran, Iran.
Shojaosadati
Seyed Abbas
SA
Karbasy
Seyed Mojtaba
SM
D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D008239:Lysine / Q000302:isolation & purification*
D008239:Lysine