title: Micronucleus test in mussels Perna perna fed with the toxic dinoflagellate Prorocentrum lima.
abstract: Micronucleus (MN) and other nuclear abnormalities have been measured in the hemocytes of mussels Perna perna to verify whether feeding mussels with different concentrations of Prorocentrum lima results in accumulation of levels of okadaic acid (OA) capable of inducing genotoxic effects at the chromosome level, as evidenced by micronuclei and nuclear abnormalities. Four groups of 12 mussels housed individually in aquaria containing filtered seawater were fed with different concentrations of P. lima. Another group collected directly from the production area served as outdoor control. A significantly higher frequency of MN and nuclear lesions was observed in hemocytes from the groups fed P. lima.
authors.affiliation: Department of Environmental Engineering, Toxicological Laboratory of the Federal University of Santa Catarina, Brazil.
authors.forename: C R Carvalho
authors.initials: CR
authors.lastname: Pinto-Silva
authors.forename: E E
authors.initials: EE
authors.lastname: Creppy
authors.forename: W G
authors.initials: WG
authors.lastname: Matias
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D002875:Chromosomes / Q000187:drug effects / Q000235:genetics; D004141:Dinoflagellida / Q000737:chemistry*; D006434:Hemocytes / Q000187:drug effects / Q000648:ultrastructure; D008387:Marine Toxins / Q000633:toxicity; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D019319:Okadaic Acid / Q000378:metabolism
chemical_list: D008387:Marine Toxins; D019319:Okadaic Acid