title: Dynamics of anti-M antibody response in a mouse model following intranasal infection with group A Streptococcus M-18.
abstract: Dynamics of anti-M antibody response following intranasal infection with group A Streptococcus (GAS) M-18 were investigated in a Swiss albino mouse model. Mice arranged in three groups were inoculated intranasally with 2.0x10(7) c.f.u. ml-1 of GAS M-18 on 1, 2 alternate and 3 alternate days. Plasma collected from the retro-orbital plexus was tested for antibodies by an in-house indirect ELISA. The antibody titres of the plasma samples varied from 1 : 8 to 1 : 1024 in the 1 day dose, from 1 : 4 to 1 : 256 in the 2 day dose and from 1 : 4 to 1 : 128 in the 3 day dose. Peak titres were seen on day 42 or 56 and in all cases the titres had declined by day 84. Swiss albino mouse can thus serve as a useful animal model to study different aspects of type-specific anti-M immune responses against GAS disease when designing candidate streptococcal vaccines.
authors.affiliation: Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore - 632004, Tamil Nadu, India 2Department of Microbiology, Pushpagiri Medical College, Tiruvalla - 689 101, Kerala, India.
authors.forename: Paul
authors.initials: P
authors.lastname: Gladstone
authors.affiliation: Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore - 632004, Tamil Nadu, India 2Department of Microbiology, Pushpagiri Medical College, Tiruvalla - 689 101, Kerala, India.
authors.forename: George
authors.initials: G
authors.lastname: Varghese
authors.affiliation: Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore - 632004, Tamil Nadu, India 2Department of Microbiology, Pushpagiri Medical College, Tiruvalla - 689 101, Kerala, India.
authors.forename: K N
authors.initials: KN
authors.lastname: Brahmadathan
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009666:Nose / Q000382:microbiology; D013290:Streptococcal Infections / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial