AtTBP2 and AtTRP2 in Arabidopsis encode proteins that bind plant telomeric DNA and induce DNA bending in vitro.
Telomeric DNA-binding proteins (TBPs) are crucial components that regulate the structure and function of eukaryotic telomeres and are evolutionarily conserved. We have identified two homologues of AtTBP1 (for Arabidopsis thaliana telomeric DNA binding protein 1), designated as AtTBP2 and AtTRP2, which encode proteins that specifically bind to the telomeric DNA of this plant. These proteins show extensive homology with other known plant TBPs. The isolated C-terminal segments of these proteins were capable of sequence-specific binding to duplex telomeric plant DNA in vitro. DNA bending assays using the Arabidopsis TBPs revealed that AtTBP1 and AtTBP2 have DNA-bending abilities comparable to that of the human homologue hTRF1, and higher than those of AtTRP1 and AtTRP2.
Hwang
Moo Gak
MG
Department of Biology, Yonsei University, Sinchon-Dong, Seodaemun-Ku, Seoul, 120-749 Republic of Korea.
Kim
Kyunghye
K
Lee
Won-Kyung
WK
Cho
Myeon Haeng
MH
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001761:Bleomycin / Q000633:toxicity; D015152:Blotting, Northern; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D005982:Glutathione Transferase; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics* / Q000378:metabolism; D034501:Telomere-Binding Proteins / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D017931:DNA Primers; C495844:TBP2 protein, Arabidopsis; D034501:Telomere-Binding Proteins; D001761:Bleomycin; D004247:DNA; D005982:Glutathione Transferase