title: The effect of Virkon S fogging on survival of Salmonella enterica and Staphylococcus aureus on surfaces in a veterinary teaching hospital.
abstract: The objective of the study was to determine the disinfection efficacy of aerosolizing (cold fogging) Virkon S on survival of Stahpylococcus aureus and Salmonella enterica on different surfaces. Two experiments were conducted in different locations. Salmonella enterica and S. aureus were grown in broth culture and then seeded into pre-marked areas in each location and allowed to dry. Virkon S (1%) was aerosolized into the rooms (approximately 1L of per 30 m(3)). Samples were collected pre- and post-fogging for quantitative cultures to evaluate the efficacy of aerial disinfection. The reduction of S. enterica or S. aureus counts ranged from 3.40 to 0.95 log(10) (Salmonella) or 4.92 to 0.02 log(10) (Staphylococcus). The greatest reduction was evident in samples collected from non-porous horizontal surfaces, which were not obstructed from the air flow. These results indicate that fogging with Virkon S could be beneficial in routine disinfection of pre-cleaned surfaces. The benefits of routine use of cold fogging with Virkon S in veterinary hospital settings would include its wide-range antimicrobial action and minimal working-men power required to disinfect large areas. Also, fogging would potentially minimize microbial contamination in the hard to reach areas.
authors.affiliation: Animal Population Health Institute, Colorado State University, Fort Collins, CO, USA.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dunowska
authors.forename: Paul S
authors.initials: PS
authors.lastname: Morley
authors.forename: Doreene R
authors.initials: DR
authors.lastname: Hyatt
mesh_terms: D000336:Aerosols; D000818:Animals; D015169:Colony Count, Microbial / Q000662:veterinary; D003428:Cross Infection / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D004202:Disinfectants / Q000494:pharmacology*; D004783:Environmental Microbiology; D006762:Hospitals, Animal / Q000592:standards*; D010545:Peroxides / Q000494:pharmacology*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control; D019779:Salmonella enterica / Q000187:drug effects* / Q000254:growth & development; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013464:Sulfuric Acids / Q000494:pharmacology*; D016896:Treatment Outcome
chemical_list: D000336:Aerosols; D004202:Disinfectants; D010545:Peroxides; D013464:Sulfuric Acids; C071517:monoperoxysulfate