title: Bioaccumulation and toxicity of dissolved heavy metals from the Guadalquivir Estuary after the Aznalcóllar mining spill using Ruditapes philippinarum.
abstract: The shore clam Ruditapes philippinarum was used as a biomonitoring organism to measure the potential impact that the mining spill in the Guadalquivir Estuary (SW, Spain) in 1998 may have exerted on local biota. Individuals were exposed to dissolved cadmium, copper, and zinc at concentrations found in local waters after the spill (3 microg x L(-1) Cd, 15 microg x L(-1) Cu, 700 microg x L(-1) Zn) at two salinity values: 10 and 35. Residues of metals were measured in gill and digestive gland, together with metallothioneins in the digestive gland and histopathological lesions in gill, digestive gland, and gonad tissues over time. Heavy metals Zn and Cd associated with the mining spill, were bioaccumulated in clam tissues, associated with the activation of metallothioneins, and related to the histopathological lesions measured at all the clam tissues. The heavy metal Cu not related to the spill was not directly associated with effects measured. The bioaccumulation and adverse effects associated with Cd and Zn were significantly higher at low salinity (10) than at high salinity (35) values.
authors.affiliation: Departamento de Química Física, Facultad de Cc. del Mar y Ambientales, Campus Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain.
authors.forename: M Laura
authors.initials: ML
authors.lastname: Martín-Díaz
authors.forename: Julián
authors.initials: J
authors.lastname: Blasco
authors.forename: Marisa
authors.initials: M
authors.lastname: González de Canales
authors.forename: Diego
authors.initials: D
authors.lastname: Sales
authors.forename: T Angel
authors.initials: TA
authors.lastname: DelValls
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000502:physiology*; D002104:Cadmium / Q000493:pharmacokinetics* / Q000633:toxicity*; D003300:Copper / Q000493:pharmacokinetics* / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods; D012623:Seawater / Q000737:chemistry; D012965:Sodium Chloride; D013030:Spain; D014018:Tissue Distribution; D015032:Zinc / Q000493:pharmacokinetics* / Q000633:toxicity*
chemical_list: D002104:Cadmium; D012965:Sodium Chloride; D003300:Copper; D015032:Zinc