Selective preparation of N-acetyl-D-glucosamine and N,N'-diacetylchitobiose from chitin using a crude enzyme preparation from Aeromonas sp.
A bacterium, Aeromonas sp. GJ-18, having strong chitinolytic activity was isolated from coastal soil and used for crude enzyme preparations. This enzyme preparation contained N-acetyl-D-glucosaminidase and N,N'-diacetylchitobiohydrolase. N-Acetyl-D-glucosaminidase was inactive above 50 degrees C, but N,N'-diacetylchitobiohydrolase was stable at this temperature. Utilizing the temperature sensitivities of the chitin degradation enzymes in crude enzyme preparation, N-acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose [(GlcNAc)(2)] were selectively produced from chitin. At 45 degrees C, GlcNAc was produced as a major hydrolytic product (94% composition) with a yield of 74% in 5 d, meanwhile at 55 degrees C (GlcNAc)(2) was the major product (86%) with a yield of 35% within 5 d.
Kuk
Ju Hee
JH
Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, Korea.
Jung
Woo Jin
WJ
Hyun Jo
Gyung
G
Ahn
Joon Seob
JS
Kim
Kil Yong
KY
Park
Ro Dong
RD
D000117:Acetylglucosamine / Q000096:biosynthesis*; D000333:Aeromonas / Q000201:enzymology*; D002686:Chitin / Q000378:metabolism*; D004187:Disaccharides / Q000096:biosynthesis*; D005959:Glucosidases / Q000378:metabolism*; D006868:Hydrolysis
D004187:Disaccharides; D002686:Chitin; C010913:N,N-diacetylchitobiose; D005959:Glucosidases; C505019:N,N'-diacetylchitobiohydrolase, Aeromonas; D000117:Acetylglucosamine