title: Mitochondrial DNA hypervariable region 1 polymorphism in Singapore Chinese population.
abstract: Sequence polymorphisms of hypervariable region 1 were analyzed in 100 unrelated Singaporean Chinese. Ninety-five different haplotypes resulting from 113 variable sites were found between nucleotide positions 16045 and 16364. Single nucleotide polymorphism at nucleotide positions 16223, 16045, 16129, 16362 and 16189 was amongst the five highest frequencies observed in the sequences, whilst the most frequent haplotype was 16045-16223. Based on polymorphic sites observed at HV1, haplogroups A, F1a, M7b1, B5a and D4b were the most commonly observed clusters. The haplotype, nucleotide diversity and the average number of nucleotide differences were found to be 0.999, 0.028 and 9.082, respectively. The cytosine-stretch region located around nucleotide position 16189 was observed in 22% of this population sample. Transitions were found to be more predominant than transversions.
authors.affiliation: NIE Biocomputational Laboratory, Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, Singapore, Singapore 637616.
authors.forename: Cheng-Yap
authors.initials: CY
authors.lastname: Shee
authors.forename: Michelle S M
authors.initials: MS
authors.lastname: Chong
authors.forename: Irene
authors.initials: I
authors.lastname: Ng
authors.forename: Tet-Fatt
authors.initials: TF
authors.lastname: Chia
mesh_terms: D022801:Complementarity Determining Regions / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D004272:DNA, Mitochondrial / Q000032:analysis*; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012846:Singapore
chemical_list: D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial