[Effect of puerarin and daidzein on proliferating vascular smooth muscle cells].
OBJECTIVE
To observe the different effects of Puerarin and Daidzein on the expression of proliferating vascular smooth muscle cells, and to discuss the mechanism.


METHODS
MT was used to detect the state of VSMC (vascular smooth muscle cell) activity. The expression levels of Survivin, Bcl-xl, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) were analyzed quantitatively by reverse transcriptase polymerase chain reaction (Rt-PCR).


RESULTS
Compared with Puerarin groups, VSMC activity in daidzein groups was lower, and the ratio of Bax/Gapdh/Bcl-xl/Gapdh was higher.


CONCLUSIONS
The inhibition effect of daidzein on VSMC proliferation is stronger than that of puerarin.
Han
Jing
J
Dongzhimen Hospital, University of Traditional Chinese Medicine, Beijing 100029, China.
Wang
Wei
W
Wang
Lu-ya
LY
Liu
Shu
S
Kang
Tie-duo
TD
D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D009363:Neoplasm Proteins; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D029908:Pueraria / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077022:Survivin; D014665:Vasodilator Agents / Q000494:pharmacology; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
C494826:BAX protein, human; C494811:BCL2L1 protein, human; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D007529:Isoflavones; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D000077022:Survivin; D014665:Vasodilator Agents; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C004742:daidzein; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C033607:puerarin