Molecular genetic methods in diagnosis and direct characterization of acute bacterial central nervous system infections.
Acute bacterial infection of the central nervous system requires rapid and adequate management. Etiological diagnosis is hence crucial. Moreover, the epidemic threat of certain bacteria necessitates a reliable characterization of the involved bacterial strains to follow the spread of epidemic strains. Conventional identification and characterization of etiological agents are basically based on culture and identification of bacterial markers most frequently by serological assays. Molecular identification and characterization of bacteria have been employed. They provide more reliable analysis of bacterial isolates. Molecular methods for non-culture diagnosis of bacterial infections have recently been developed. In many cases, the molecular assays have decreased the identification time of positive cultures and rescued detection of pathogens in culture-negative clinical samples.
Taha
Muhamed-Kheir
MK
Neisseria Unit and National Reference Center for Meningococci, Institut Pasteur, Paris, France. mktaha@pasteur.fr
Olcén
Per
P
D000208:Acute Disease; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D002494:Central Nervous System Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping