[Chlamydia pneumoniae infection in patients with advanced carotid atherosclerosis].
Despite the growing number of scientific reports showing different markers of infection caused by Chlamydia pneumoniae in patients with advanced atherosclerosis, there is still no clear confirmation of a pathogenetic link between this infection and atherogenesis. The aim of the present study was to investigate the presence C. pneumoniae DNA in peripheral blood mononuclear cells and carotid endarterectomy samples obtained from patients with advanced atherosclerosis according to the presence specific antibodies against C. pneumoniae in serum. The levels of IgG, IgM and IgA antibodies to C. pneumoniae were determined by enzyme immunoassay (EIA) in sera of 36 patients with advanced atherosclerosis undergoing carotid endarterectomy. The presence of C. pneumoniae DNA in the carotid samples and in peripheral blood mononuclear cells was investigated by a nested polymerase chain reaction (PCR). We have not confirm the presence of C. pneumoniae DNA either in the carotid endarterectomy samples or in peripheral blood mononuclear cells, both in patients having the specific antibodies against C. pneumoniae and in seronegative patients. In the studied group of patients with advanced carotid atherosclerosis there was no correlation between the presence of specific antibodies against C. pneumoniae and the presence of C. pneumoniae DNA in endarterectomy samples and peripheral blood mononuclear cells.
Karyński
Michał
M
Narodowy Instytut Zdrowia Publicznego, Warszawa. paolo@cls.edu.pl
Słowiński
Piotr
P
Grzesiowski
Paweł
P
Fiett
Janusz
J
Podsiadły
Edyta
E
Walory
Jarosław
J
Tylewska-Wierzbanowska
Stanisława
S
Staszkiewicz
Walerian
W
Hryniewicz
Waleria
W
D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D002340:Carotid Artery Diseases / Q000382:microbiology*; D023521:Chlamydophila Infections / Q000150:complications*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial