Impaired flow-induced dilation in mesenteric resistance arteries from receptor protein tyrosine phosphatase-mu-deficient mice.
The transmembrane receptor-like protein tyrosine phosphatase-mu (RPTPmu) is thought to play an important role in cell-cell adhesion-mediated processes. We recently showed that RPTPmu is predominantly expressed in the endothelium of arteries and not in veins. Its involvement in the regulation of endothelial adherens junctions and its specific arterial expression suggest that RPTPmu plays a role in controlling arterial endothelial cell function and vascular tone. To test this hypothesis, we analyzed myogenic responsiveness, flow-induced dilation, and functional integrity of mesenteric resistance arteries from RPTPmu-deficient (RPTPmu(-/-)) mice and from wild-type littermates. Here, we show that cannulated mesenteric arteries from RPTPmu(-/-) mice display significantly decreased flow-induced dilation. In contrast, mechanical properties, myogenic responsiveness, responsiveness to the vasoconstrictors phenylephrine or U-46619, and responsiveness to the endothelium-dependent vasodilators methacholine or bradykinin were similar in both groups. Our results imply that RPTPmu is involved in the mechanotransduction or accessory signaling pathways that control shear stress responses in mesenteric resistance arteries.
Koop
Elianne A
EA
Department of Medical Oncology, University Medical Centre Utrecht, Utrecht, The Netherlands.
Gebbink
Martijn F B G
MF
Sweeney
Terrence E
TE
Mathy
Marie-Jeanne
MJ
Heijnen
Harry F G
HF
Spaan
Jos A E
JA
Voest
Emile E
EE
VanBavel
Ed
E
Peters
Stephan L M
SL
D000818:Animals; D001794:Blood Pressure; D015870:Gene Expression; D006339:Heart Rate; D007763:Lac Operon; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008638:Mesenteric Arteries / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D014661:Vasoconstriction / Q000502:physiology; D014664:Vasodilation / Q000502:physiology*
D017027:Protein Tyrosine Phosphatases; C516860:Ptprm protein, mouse; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2