Mineral nutrition and adventitious rooting in microcuttings of Eucalyptus globulus.
We characterized the adventitious rooting response of Eucalyptus globulus Labill. to various concentrations of calcium, nitrogen, phosphorus, iron, manganese, zinc, boron and copper. The parameters analyzed were percent rooting, root number, root length and mean rooting time. Root number and root length were significantly affected by mineral nutrition, whereas mean rooting time and rooting percentage seemed to be closely related to auxin availability. Root number was affected by calcium, nitrogen source and zinc, whereas root length was influenced by concentrations of phosphorus, iron and manganese, and by nitrogen source. Based on these results, we evaluated various combinations of several concentrations of these minerals in each rooting phase. Cuttings that were rooted in an optimized mineral nutrient medium and acclimatized to ex-vitro conditions for two months showed significantly higher survival after transplanting and drought stress than cuttings rooted in basal medium and treated in the same way.
Schwambach
Joséli
J
Centro de Biotecnologia UFRGS (Programa de Pós-Graduação em Biologia Celular e Molecular), C.P. 15005, CEP 91501-970, Porto Alegre, RS, Brazil.
Fadanelli
Cristina
C
Fett-Neto
Arthur G
AG
D003470:Culture Media / Q000737:chemistry*; D004602:Elements; D005052:Eucalyptus / Q000254:growth & development*; D018517:Plant Roots / Q000254:growth & development*; D046509:Tissue Culture Techniques
D003470:Culture Media; D004602:Elements