title: Identification and genomic characterization of a new virus (Tymoviridae family) associated with citrus sudden death disease.
abstract: Citrus sudden death (CSD) is a new disease that has killed approximately 1 million orange trees in Brazil. Here we report the identification of a new virus associated with the disease. RNAs isolated from CSD-affected and nonaffected trees were used to construct cDNA libraries. A set of viral sequences present exclusively in libraries of CSD-affected trees was used to obtain the complete genome sequence of the new virus. Phylogenetic analysis revealed that this virus is a new member of the genus Marafivirus. Antibodies raised against the putative viral coat proteins allowed detection of viral antigens of expected sizes in affected plants. Electron microscopy of purified virus confirmed the presence of typical isometric Marafivirus particles. The screening of 773 affected and nonaffected citrus trees for the presence of the virus showed a 99.7% correlation between disease symptoms and the presence of the virus. We also detected the virus in aphids feeding on affected trees. These results suggest that this virus is likely to be the causative agent of CSD. The virus was named Citrus sudden death-associated virus.
authors.affiliation: Alellyx Applied Genomics, Techno Park (Rod. Anhanguera Km 104), Rua James Clerk Maxwell 320, 13069-380 Campinas, SP, Brazil.
authors.forename: Walter
authors.initials: W
authors.lastname: Maccheroni
authors.forename: Marcos C
authors.initials: MC
authors.lastname: Alegria
authors.forename: Christian C
authors.initials: CC
authors.lastname: Greggio
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Piazza
authors.forename: Rachel F
authors.initials: RF
authors.lastname: Kamla
authors.forename: Paula R A
authors.initials: PR
authors.lastname: Zacharias
authors.forename: Moshe
authors.initials: M
authors.lastname: Bar-Joseph
authors.forename: Elliot W
authors.initials: EW
authors.lastname: Kitajima
authors.forename: Laura C
authors.initials: LC
authors.lastname: Assumpção
authors.forename: Giovana
authors.initials: G
authors.lastname: Camarotte
authors.forename: Jussara
authors.initials: J
authors.lastname: Cardozo
authors.forename: Elaine C
authors.initials: EC
authors.lastname: Casagrande
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ferrari
authors.forename: Sulamita F
authors.initials: SF
authors.lastname: Franco
authors.forename: Poliana F
authors.initials: PF
authors.lastname: Giachetto
authors.forename: Alessandra
authors.initials: A
authors.lastname: Girasol
authors.forename: Hamilton
authors.initials: H
authors.lastname: Jordão
authors.forename: Vitor H A
authors.initials: VH
authors.lastname: Silva
authors.forename: Leonardo C A
authors.initials: LC
authors.lastname: Souza
authors.forename: Carlos I
authors.initials: CI
authors.lastname: Aguilar-Vildoso
authors.forename: Almir S
authors.initials: AS
authors.lastname: Zanca
authors.forename: Paulo
authors.initials: P
authors.lastname: Arruda
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Kitajima
authors.forename: Fernando C
authors.initials: FC
authors.lastname: Reinach
authors.forename: Jesus A
authors.initials: JA
authors.lastname: Ferro
authors.forename: Ana C R
authors.initials: AC
authors.lastname: da Silva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000821:virology; D001483:Base Sequence; D001938:Brazil; D036022:Capsid Proteins / Q000235:genetics; D002957:Citrus / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D046830:Tymoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral