Comparison of the effect of amphotericin B desoxycholate and amphotericin B colloidal dispersion on renal functions and renal morphology in rats.
OBJECTIVE
Amphotericin B (AmB) desoxycholate remains as one of the most efficacious agents currently available for the treatment of systemic fungal infections; however, amphotericin B colloidal dispersion (ABCD) has been developed because of AmB desoxycholate nephrotoxicity. The goal of our study was to compare the effect of administration of AmB desoxycholate and ABCD on renal functions and renal morphology in rats.


RESULTS
Amophotericin B desoxycholate as well as ABCD causes damage to renal tubuli and polyuria. Amophotericin desoxycholate causes considerably more severe damage to tubuli than ABCD, but the morphological damage to renal glomeruli is minimal in both formulas. In tubular cells, AmB desoxycholate causes severe damage to mitochondria, vacuolation of cytoplasm, and increased values of volume density of peroxisomes.


CONCLUSIONS
None of these formulas causes a decrease in glomerular filtration in rats when animals are properly hydrated.
Doubek
Michael
M
Department of Internal Medicine - Haematology/Oncology, University Hospital, 62500 Brno, Czech Republic. mdoubek@fnbrno.cz
Mayer
Jiri
J
Lauschova
Irena
I
Scheer
Peter
P
Krejcirova
Lenka
L
Horky
Drahomir
D
Doubek
Jaroslav
J
D000666:Amphotericin B / Q000633:toxicity*; D000818:Animals; D000935:Antifungal Agents / Q000633:toxicity*; D003593:Cytoplasm / Q000187:drug effects / Q000473:pathology; D003840:Deoxycholic Acid / Q000633:toxicity*; D004338:Drug Combinations; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*; D008138:Loop of Henle / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D014617:Vacuoles / Q000187:drug effects / Q000473:pathology
D000935:Antifungal Agents; D004338:Drug Combinations; D003840:Deoxycholic Acid; D000666:Amphotericin B; C059765:amphotericin B, deoxycholate drug combination