Determination of biodegradation products from sulfonated dyes by Pleurotus ostreatususing capillary electrophoresis coupled with mass spectrometry.
Microbial treatment of environmental pollutants including dyes with white rot fungi has received wide attention as a potential alternative for conventional methods in wastewater treatment. The degradation products from dyes and mechanism underlying fungal degradation of dyes is desirable to be understood. Capillary electrophoresis coupled with mass spectrometry (CE-MS) was used in this study to determine biodegradation products of 4-[(4-hydroxyphenyl)azo]-benzenesulfonic acid, sodium salt (4HABA) and Acid Orange 7 (C.I. 15510), produced by a white rot fungus, Pleurotus ostreatus. Two major degradation products, benzenesulfonic acid and 4-hydroxy-benzenesulfonic acid, from both sulfonated compounds, were identified and their kinetic profiles in biodegradation were followed by CE-MS. Another product, 1,2-naphthoquinone, from Acid Orange 7 was identified using HPLC. Formation of these products in fungal degradation is discussed.
Zhao
Xueheng
X
Department of Biology, Jackson State University, 39217, Jackson, MS 39217, USA. xueheng.zhao@jsums.edu
Lu
Yiping
Y
Hardin
Ian
I
D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D020076:Pleurotus / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013451:Sulfonic Acids / Q000737:chemistry*
D004396:Coloring Agents; D013451:Sulfonic Acids