Injectable bone using chitosan-alginate gel/mesenchymal stem cells/BMP-2 composites.
OBJECTIVE
Several injectable materials have been used as osteogenic bone substitutes. However, none has gained universal acceptance. This study was performed to investigate whether or not chitosan-alginate gel/mesenchymal stem cells/bone morphogenetic protein-2 composites are potentially injectable materials for new bone formation.


METHODS
The composites were injected into the subcutaneous space on the dorsum of nude mice to investigate new bone tissue formation. The composites were examined histologically over a 12-week period.


RESULTS
The composites injected into the mouse were able to stimulate new bone formation, which was trabecular in type.


CONCLUSIONS
This study showed that chitosan-alginate gel/MSCs/BMP-2 composites could become clinically useful injectable materials to generate new bone.
Park
Dong-Joon
DJ
Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, South Korea.
Choi
Byung-Ho
BH
Zhu
Shi-Jiang
SJ
Huh
Jin-Young
JY
Kim
Byung-Young
BY
Lee
Seoung-Ho
SH
D000464:Alginates / Q000008:administration & dosage; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000008:administration & dosage* / Q000737:chemistry; D048271:Chitosan / Q000008:administration & dosage; D005782:Gels; D020723:Glucuronic Acid / Q000008:administration & dosage; D006603:Hexuronic Acids / Q000008:administration & dosage; D059630:Mesenchymal Stem Cells / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D016212:Transforming Growth Factor beta / Q000008:administration & dosage
D000464:Alginates; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D005782:Gels; D006603:Hexuronic Acids; D016212:Transforming Growth Factor beta; D020723:Glucuronic Acid; D048271:Chitosan