Spectrally resolved femtosecond 2-colour 3-pulse photon echoes: a new spectroscopic tool to study molecular dynamics.
We present a new multidimensional femtosecond spectroscopy technique based on spectrally resolved 2-colour 3-pulse photon echoes for investigating molecular dynamics in a variety of systems including proteins. In this technique the sample is illuminated by two femtosecond 'pump' pulses with wave vectors k1, k2 and wavelength lambda(pump) and a femtosecond 'probe' pulse with wave vector k3 and wavelength lambda(probe). Nonlinear signals are generated in the phase-matching directions k4 = - k1 + k2 + k3 and k6 = - k3 + k1 + k2. These signals are analysed in spectrometers equipped with CCD detectors and the spectra of the signals are recorded for various values of (i) the delay t12 between pulses 1 and 2, (ii) the delay t23 between pulses 2 and 3, and (iii) the wavelengths lambda(pump), lambda(probe). The technique has been used for studying vibrational and electronic dynamics of dye molecules, such as cresyl violet in methanol, and ultra-fast transient processes that occur during the photo-dissociation of carbonmonoxy myoglobin (MbCO) into myoglobin (Mb) and CO.
Dao
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LV
Centre for Atom Optics and Ultrafast Spectroscopy, School of Biophysical Sciences and Electrical Engineering, Swinburne University of Technology, Melbourne, Australia. dvlap@swin.edu.au
Lincoln
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Do
M T T
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Eckle
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Lowe
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Hannaford
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D000465:Algorithms; D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D003116:Color; D008967:Molecular Biology / Q000379:methods*; D009038:Motion; D013057:Spectrum Analysis / Q000379:methods*; D013997:Time Factors
D001704:Biopolymers