Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.
Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, beta-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.
Radonić
Aleksandar
A
Charité - CCM, Medizinische Klinik II m.S. Hämatologie/Onkologie, Berlin, Germany. aleksandar.radonic@charite.de
Thulke
Stefanie
S
Bae
Hi-Gung
HG
Müller
Marcel A
MA
Siegert
Wolfgang
W
Nitsche
Andreas
A
D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D005814:Genes, Viral / Q000235:genetics*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011212:Poxviridae / Q000235:genetics / Q000302:isolation & purification*; D012015:Reference Standards; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification*; D035181:TATA-Box Binding Protein / Q000378:metabolism; D014779:Virus Replication; D015005:Yellow fever virus / Q000235:genetics / Q000302:isolation & purification*
D035181:TATA-Box Binding Protein; D019696:Peptidylprolyl Isomerase