[Analytical methodology for drug-protein binding studies].
The qualitative and quantitative aspects of several novel methods for drug-protein binding studies, which include chromatography, capillary electrophoresis, mass spectrometry, nuclear magnetic resonance, cloud-point extraction and microdialysis as well as those of the traditional approaches such as equilibrium dialysis and ultrafiltration are reviewed. The advantages and limitations of these methods are discussed.
Zhou
Dawei
D
Pharmaceutical College, Shenyang Pharmaceutical University, Shenyang 110016, China. dawei1204@yahoo.com
Li
Ledao
L
Li
Famei
F
D002845:Chromatography / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D004364:Pharmaceutical Preparations; D011506:Proteins