title: [Determination of ivermectin in pig serum by high performance liquid chromatography].
abstract: OBJECTIVE
  To establish a high performance liquid chromatographic method for the determination of micro-amount of ivermectin in pig serum.
  
  
  METHODS
  Ivermectin in pig serum was extracted with ethyl acetate after the serum protein was precipitated with 0.05% metaphosphoric acid-methanol in the ratio of 7:3 (V/V). Then the sample was centrifuged at 4000 r/min for 5 min and the supernate was evaporated to dryness with rotary vacuum evaporator. The residue was dissolved with 0.20 ml of methanol as the sample solution for HPLC analysis. HPLC column used was a phenomenex C18 (5 microm, 250 mm x 4.6 mm) with a same type of guard column. Mobile phase consisted of methanol and water in the ratio of 90:10 (V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 245 nm.
  
  
  RESULTS
  The linear range of the method was found to be 0.010-20 mg/L and its detection limit was 0.010 mg/L. The relative standard deviation of the method was 0.78%-3.82% and the recoveries varied from 94.0% to 100.0%, with an average recovery of 97.3%.
  
  
  CONCLUSIONS
  This method is simple, reproducible, accurate and suitable to the determination of micro-amount of ivermectin in pig serum.
authors.affiliation: Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Jian-hong
authors.initials: JH
authors.lastname: Zhao
authors.forename: Cheng-jun
authors.initials: CJ
authors.lastname: Sun
authors.forename: Li-sha
authors.initials: LS
authors.lastname: Mao
authors.forename: Zong-ning
authors.initials: ZN
authors.lastname: Yin
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Li
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007559:Ivermectin / Q000097:blood*; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D000871:Anthelmintics; D007559:Ivermectin