[Effects of a novel recombinant polypeptide from the sea anemone Anthopleura sp. on left ventricular function in dogs with acute cardiac insufficiency].
OBJECTIVE
To observe the effects of recombinant hk2a, a novel neurotoxin from the sea anemone Anthopleura sp., on left ventricular function of dogs with acute cardiac insufficiency.


METHODS
Canine models of acute cardiac insufficiency were established by rapid ventricular pacing, in which the left ventricular ejection fraction (LVEF) was measured by Acuson ultrasound systems (Sequoia 512) at 0, 5, 15, 30 and 60 min, respectively, after intravenous injection of 30 microg/kg recombinant hk2a. The response of the canine models to hk2a treatments was observed in comparison with that of the dogs treated with Cedilanid (as positive control) and saline (as negative control).


RESULTS
Intravenous injection of recombinant hk2a caused an immediate and significant increase in LVEF in the canine models of acute cardiac insufficiency (P<0.05), and the effect maintained for more than 30 min without significant effect on heart rate. Recombinant hk2a possessed such merits as quicker onset and greater potency in comparison with Cedilanid.


CONCLUSIONS
Recombinant hk2a may significantly increase LVEF of the dogs with acute cardiac insufficiency.
Ouyang
Ping
P
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. ypou@fimmu.com
Xie
Jin-guo
JG
Liu
Jian
J
Xiao
Wen-xing
WX
Liu
Yan-bo
YB
Wang
Lei
L
Liang
Dong
D
Wang
Yong-hua
YH
Xu
Ding-li
DL
Liu
Yi-li
YL
Xu
An-long
AL
D000818:Animals; D002303:Cardiac Output, Low / Q000188:drug therapy* / Q000503:physiopathology; D003064:Cnidarian Venoms / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D004285:Dogs; D005260:Female; D008297:Male; D009498:Neurotoxins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D012615:Sea Anemones / Q000737:chemistry*; D016277:Ventricular Function, Left / Q000187:drug effects*
D003064:Cnidarian Venoms; D009498:Neurotoxins; D011994:Recombinant Proteins