An attempt to protect winter wheat against Fusarium culmorum by the use of rhizobacteria Pseudomonas fluorescens and Bacillus mycoides.
Inoculation of wheat seeds with two strains of Pseudomonas fluorescens (III107 and II21) and two strains of Bacillus mycoides (JC192 and K184) isolated from winter wheat roots, as well as with one strain of P fluorescens (ID13) isolated from oat roots, reduced the negative influence of Fusarium culmorum on winter wheat in a 28 day pot experiment. The bacterial strains (especially III107 and chitinolytic JC 192) markedly increased the plant seedlings emergence and the plant biomass (the shoots weight up to 252%, and the roots weight up to 229%) in comparison to the experimental series with E culmorum alone. Also in a microplot experiment the yield of grain and straw of winter wheat, inoculated with the bacterial strains (especially JC192 and III107) and growing in soil contaminated with F. culmorum, was higher (the grain yield up to 120%, and the straw yield up to 139%) than in a series with F. culmorum alone (100%). In both experiments the highly cyanogenic strain II21 was least effective. A linear correlation (r = 0.926) and a rank Spearman's correlation (rSp = 0.991), both significant at p<0.01, between the weight of plant biomass in the pot experiment and the yield of whole shoots in the microplot experiment were found. It suggests that the same mechanisms worked in both experiments, although with different intensity.
Czaban
Janusz
J
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation, Puławy, Poland. czaban@iung.pulawy.pl
Ksiezniak
Andrzej
A
Perzyński
Andrzej
A
D001407:Bacillus / Q000502:physiology*; D005670:Fusarium / Q000254:growth & development*; D010572:Pest Control, Biological / Q000379:methods*; D011551:Pseudomonas fluorescens / Q000502:physiology*; D012639:Seeds / Q000382:microbiology; D014908:Triticum / Q000382:microbiology*