Evaluation of dynamic headspace with gas chromatography/mass spectrometry for the determination of 1,1,1-trichloroethane, trichloroethanol, and trichloroacetic acid in biological samples.
A sensitive and reproducible method is described for the analysis of trichloroacetic acid in urine and 1,1,1-trichloroethane in blood using dynamic headspace GC/MS. Samples were analyzed using the soil module of a modified purge and trap autosampler to facilitate the use of disposable purging vessels. Coefficients of variation were below 3.5% for both analytes, and response was linear in the range of 0.01-7.0 microg/ml for trichloroacetic acid and 0.9 ng/ml-2.2 microg/ml for 1,1,1-trichloroethane. Attempts at using dynamic headspace for the analysis of trichloroethanol in urine were unsuccessful.
Johns
Douglas O
DO
Department of Environmental and Occupational Health Sciences, University of Washington, Box 357234, Seattle, WA 98195-7234, USA. djohns@u.washington.edu
Dills
Russell L
RL
Morgan
Michael S
MS
D005023:Ethylene Chlorohydrin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D014238:Trichloroacetic Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D014240:Trichloroethanes / Q000032:analysis* / Q000097:blood / Q000652:urine
D014240:Trichloroethanes; C024566:1,1,1-trichloroethane; D014238:Trichloroacetic Acid; D005023:Ethylene Chlorohydrin; C005849:2,2,2-trichloroethanol