title: Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosoma japonicum after pairing.
abstract: Identification of differentially expressed proteins between the male and female worm of Schistosoma japonicum may provide new insights into the development of schistosomes, especially the molecular mechanism of female worm maturation induced by the male worm after pairing. Comparative two-dimensional gel electrophoresis (2-DE) and mass spectrometry were employed to separate and identify differentially expressed proteins between the male and female worm after pairing. Soluble and hydrophobic proteins from egg, schistosomulum (14 days), and female and male worms at adult stage (42 days) were separated by a sequential extraction method followed by 2-DE and 2-DE images were constructed. There were 1016 +/- 67, 1808 +/- 89, 1142 +/- 45 and 1288 +/- 32 spots detected for soluble proteins and 1425 +/- 108, 952 +/- 59, 847 +/- 75 and 965 +/- 69 spots for hydrophobic proteins from egg, schistosomulum, and adult stage female and male worms, respectively. The differentially and uniquely expressed proteins from male and female worms after pairing (42 days) include 41 +/- 4 and 23 +/- 2 unique spots for soluble proteins, and 11 +/- 1 and 26 +/- 3 unique spots for hydrophobic proteins, respectively. Matrix-assisted laser desorption/ionization-time of flight and electrospray ionization-tandem mass spectrometry were employed to analyze 12 unique spots from the female worm and 16 unique spots from the male worm for peptide mass fingerprinting and sequencing. The results showed that the main functions of these differentially expressed proteins were in signal transduction, metabolism and transcriptional regulation etc. Comparison of the schistosomes proteome between male and female worms may permit the identification of protein candidates for the development of vaccines or new targets for drug development against schistosomiasis.
authors.affiliation: Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Cheng
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
authors.forename: Xin-Gang
authors.initials: XG
authors.lastname: Feng
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Fu
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: Jin
authors.forename: Chun-Xiu
authors.initials: CX
authors.lastname: Yuan
authors.forename: Yuan-Cong
authors.initials: YC
authors.lastname: Zhou
authors.forename: You-Min
authors.initials: YM
authors.lastname: Cai
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015801:Helminth Proteins / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D008018:Life Cycle Stages; D008297:Male; D013058:Mass Spectrometry; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000737:chemistry* / Q000254:growth & development*; D016622:Silver Staining; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015801:Helminth Proteins; D020543:Proteome