title: Flavonoids from the leaves of Diospyros kaki reduce hydrogen peroxide-induced injury of NG108-15 cells.
abstract: Flavonoids from the leaves of Diospyros kaki L (FLDK-P70) are main therapeutic components of NaoXingQing, which is a novel and patented Traditional Chinese Medicine drug used for the treatment of syndrome of apoplexy for years in China. The present study investigated the effects of FLDK-P70 on hydrogen peroxide (H2O2)-induced apoptosis-like damage of NG108-15 cells. Pretreatment of cells with FLDK-P70 alleviated H2O2-induced cell injury and apoptosis by upregulating Bcl-2 expression and improving redox imbalance as indicated by the alleviation of the decline in the intracellular endogenous antioxidants: glutathione and glutathione peroxidase as well as catalase, with the decrease of the leak of lactate dehydrogenase and the reduction of the accumulation of malondialdehyde. These results indicate that FLDK-P70 may be potentially used in the prevention and treatment of ischemia/reperfusion injury and other neurodegenerative disease. Upregulating bcl-2 and improving cellular redox state by FLDK-P70 may play critical roles in attenuating oxidative injury.
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry and Center for Biopharmaceutical Research, School of Life Sciences, Sun Yatsen (Zhongshan) University, 135 W. XinGang Road, Guangzhou, 510275, PR China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Bei
authors.forename: Wenlie
authors.initials: W
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Anlong
authors.initials: A
authors.lastname: Xu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D029788:Diospyros / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D009447:Neuroblastoma / Q000188:drug therapy / Q000473:pathology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D018515:Plant Leaves / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D019253:Proto-Oncogene Proteins c-bcl-2; D006861:Hydrogen Peroxide