title: Accumulation of biologically active furanocoumarins in Ruta graveolens ssp. divaricata (Tenore) Gams in vitro culture.
abstract: The study was designed to investigate dynamics of accumulation of five linear furanocoumarins and umbelliferone in stationary liquid cultures of Ruta graveolens ssp. divaricata (Tenore) Gams during 6-week growth cycles. The contents of individual metabolites in biomass increased 1.8-3.5 times while their total content rose 2.3 times. Maximum contents of xanthotoxin, bergapten and isopimpinellin (112.3, 76.2 and 84.0mg/100g d.w., respectively) and maximum total content of all metabolites (283.4 mg/100 g d.w.), obtained on 35th culture day, are interesting from practical point of view.
authors.affiliation: Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, Kraków, Poland. mfekiert@cyf-kr.edu.pl
authors.forename: H
authors.initials: H
authors.lastname: Ekiert
authors.forename: A A
authors.initials: AA
authors.lastname: Abou-Mandour
authors.forename: F Ch
authors.initials: FCh
authors.lastname: Czygan
mesh_terms: D018533:Biomass; D002453:Cell Cycle / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D011564:Furocoumarins / Q000096:biosynthesis*; D032105:Ruta / Q000378:metabolism*; D046509:Tissue Culture Techniques; D014468:Umbelliferones / Q000378:metabolism
chemical_list: D011564:Furocoumarins; D014468:Umbelliferones; C031477:7-hydroxycoumarin