[Analysis of the E gene sequences of three dengue virus type 1 isolates in Guangdong province].
OBJECTIVE
To trace the infection sources of three dengue virus type 1 isolates found in Guangdong province, namely GD05/99, GD14/97 and GD23/95.


METHODS
According to the genomes of dengue virus type 1 S275 strain, a pair of primers was designed for amplification of the structural protein E gene of the isolated dengue virus type 1 strains by reverse transcriptase-PCR. The amplified gene fragment was then cloned into pMD18-T vector and sequenced.


RESULTS
GD14/97 and GD05/99 shared a high nucleotide homology with Cambodia (98% and 99% respectively) and may belong to the same genotype. GD23/95 had a 95% nucleotide homology with A88, higher than that with other strains. GD23\95 and A88 may belong to another genotype.


CONCLUSIONS
The dengue fever outbreak in Guangdong during 1999, 1997, and 1995 may originate from the viruses from different countries.
Tian
Xiao-dong
XD
Department of Microbiology, Institute of Military Medicine of Guangzhou Command, Guangzhou 510507, China. txd1957@Sohu.com
Liu
Jian-wei
JW
Fang
Mei-yu
MY
Jiang
Lian-hua
LH
Ren
Rui-wen
RW
Hong
Wen-yan
WY
Cheng
Gang-feng
GF
D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C072281:E protein TH Sman, Dengue virus