Improvement of electrophoretic detection of antitrypsin activity in fish blood and seminal plasma.
The original method of Uriel and Berges for detection of trypsin inhibitors lacks specificity due to masking effects of nonspecific esterases. We report a modification of this method based on inhibition of esterases in samples by phenylmethylsulfonyl fluoride (PMSF). This method can be particularly useful for characterization profiles of antitrypsin activity in seminal plasma of salmonid fish where esterases and inhibitors migrate at the same mobility.
Wojtczak
Mariola
M
Department of Semen Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Poland.
Ciereszko
Andrzej
A
D000818:Animals; D043182:Carboxylesterase / Q000037:antagonists & inhibitors; D004586:Electrophoresis / Q000379:methods*; D005399:Fishes / Q000097:blood*; D008297:Male; D010664:Phenylmethylsulfonyl Fluoride / Q000737:chemistry; D012661:Semen / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000097:blood* / Q000302:isolation & purification*
D014361:Trypsin Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D043182:Carboxylesterase