title: Investigation of bacterial diversity in Brazilian tropical estuarine sediments reveals high actinobacterial diversity.
abstract: Phylogenetic and statistical analyses of 16S rRNA gene libraries were used for the investigation of actinobacterial communities present in two tropical estuarine sediments (Santos-São Vicente estuary, Brazil). The libraries were constructed from samples collected at the brackish end of the estuary, highly hydrocarbon-contaminated, and at the marine end, uncontaminated. Clones from the marine end of the estuary were all related to sequences from non-cultured Actinobacteria and unidentified bacteria recovered from a wide range of environmental samples, whereas clones from the brackish end were mainly related to sequences from cultured Actinobacteria. Statistical analyses showed that the community recovered from the hydrocarbon-contaminated sediment sample, at the brackish end, was less diverse than the uncontaminated one, at the marine end, and that the communities from the two libraries were differently structured, suggesting that these may have not originated from the same community. The recognition of the spatial pattern of actinobacterial distribution in a natural environment is a first step towards understanding the way these communities are organized, providing valuable data for further investigations of their taxonomic and functional diversity.
authors.affiliation: Microbial Resources Division, CPQBA, State University of Campinas (UNICAMP), PO Box 6171, CEP 13081-970, Campinas, SP, Brazil. fepiza@uol.com.br
authors.forename: Fernanda Francischetti
authors.initials: FF
authors.lastname: Piza
authors.forename: Paulo Inácio
authors.initials: PI
authors.lastname: Prado
authors.forename: Gilson Paulo
authors.initials: GP
authors.lastname: Manfio
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics; D001938:Brazil; D014644:Genetic Variation; D019015:Geologic Sediments / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D013223:Statistics as Topic / Q000379:methods*
chemical_list: D012336:RNA, Ribosomal, 16S