title: Colocalization of apolipoprotein AI in various kinds of systemic amyloidosis.
abstract: Apolipoprotein AI (apoAI), a major component of high-density lipoproteins, is one of the major amyloid fibril proteins and a minor constituent of the senile plaques observed in Alzheimer's disease. We examined colocalization of apoAI in various kinds of systemic amyloidosis in this study. Forty-three of 48 formalin-fixed paraffin-embedded heart specimens with various forms of systemic amyloidosis reacted immunohistochemically with anti-human apoAI antibody. ApoAI was also detected in water-extracted amyloid material by immunoblotting. In addition, we observed colocalization of apoAI and murine amyloid A (AA) amyloidosis in human apoAI transgenic mice. This is the first report of colocalization of apoAI with amyloid deposits in various forms of human systemic amyloidosis and murine AA amyloidosis in human apoAI transgenic mice. ApoAI may not always be a major component of amyloid fibrils, even when it is present in systemic amyloid deposits.
authors.affiliation: Department of Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan. nsakata@yamaguchi-u.ac.jp
authors.forename: Naohiro
authors.initials: N
authors.lastname: Sakata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hoshii
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Makiko
authors.initials: M
authors.lastname: Kiyama
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Arai
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Omoto
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Morimatsu
authors.forename: Tokuhiro
authors.initials: T
authors.lastname: Ishihara
mesh_terms: D000686:Amyloidosis / Q000378:metabolism*; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics / Q000378:metabolism*; D009202:Cardiomyopathies / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000378:metabolism
chemical_list: D016632:Apolipoprotein A-I; D000685:Serum Amyloid A Protein