[Effect of trypsin and Triton-X 100 for decellularization of porcine aortic heart valves].
OBJECTIVE
To test two decellularization procedures for their potential of cell removal and mormal matrix preservation.


METHODS
Porcine aortic valve leaflets were treated with either 0.05% trypsin or 0.25% Triton-X 100 respertively for 48 h for decellularization and with fresh untreated valve leaflets as control. Two tissue samples from each group were stained with hematoxylin and eosin and observed light-microscopically followed by scanning electron microscopy. Ten valve leaflets in each group were measured for shrinkage temperature, tensile strength/fracture toughnes and percentage elongation.


RESULTS
Trypsin and Triton-X 100 all achieved complete decellularization but Triton-X 100 caused stronger structural alterations. No significant difference was identified between untreated and trypsin groups in shrinkage temperature, tensile strength/fracture toughnes and percentage elongation, but Triton-X 100 group showed significant difference from the other two groups.


CONCLUSIONS
Decellularization using trypsin is superior to Triton-X100 in efficiency and matrix preservation.
Wang
Ke-xue
KX
Department of Cardiac Surgery, Institute of Cardiovascular Diseases, Guangzhou 510100, China. kexuewang@tom.com
Zhang
Jing-fang
JF
Zhan
Qiu-peng
QP
Jian
Xu-hua
XH
D000818:Animals; D001021:Aortic Valve / Q000166:cytology / Q000187:drug effects*; D001705:Bioprosthesis; D006350:Heart Valve Prosthesis; D006801:Humans; D017830:Octoxynol / Q000494:pharmacology*; D011474:Prosthesis Design; D013501:Surface-Active Agents / Q000494:pharmacology; D013552:Swine; D014357:Trypsin / Q000494:pharmacology*
D013501:Surface-Active Agents; D017830:Octoxynol; D014357:Trypsin