title: AtTBP2 and AtTRP2 in Arabidopsis encode proteins that bind plant telomeric DNA and induce DNA bending in vitro.
abstract: Telomeric DNA-binding proteins (TBPs) are crucial components that regulate the structure and function of eukaryotic telomeres and are evolutionarily conserved. We have identified two homologues of AtTBP1 (for Arabidopsis thaliana telomeric DNA binding protein 1), designated as AtTBP2 and AtTRP2, which encode proteins that specifically bind to the telomeric DNA of this plant. These proteins show extensive homology with other known plant TBPs. The isolated C-terminal segments of these proteins were capable of sequence-specific binding to duplex telomeric plant DNA in vitro. DNA bending assays using the Arabidopsis TBPs revealed that AtTBP1 and AtTBP2 have DNA-bending abilities comparable to that of the human homologue hTRF1, and higher than those of AtTRP1 and AtTRP2.
authors.affiliation: Department of Biology, Yonsei University, Sinchon-Dong, Seodaemun-Ku, Seoul, 120-749 Republic of Korea.
authors.forename: Moo Gak
authors.initials: MG
authors.lastname: Hwang
authors.forename: Kyunghye
authors.initials: K
authors.lastname: Kim
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Lee
authors.forename: Myeon Haeng
authors.initials: MH
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001761:Bleomycin / Q000633:toxicity; D015152:Blotting, Northern; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D005982:Glutathione Transferase; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics* / Q000378:metabolism; D034501:Telomere-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; C495844:TBP2 protein, Arabidopsis; D034501:Telomere-Binding Proteins; D001761:Bleomycin; D004247:DNA; D005982:Glutathione Transferase