title: Histochemical analysis of glycoconjugate secretion in the alimentary canal of Anguilla anguilla L.
abstract: Conventional histochemical methods as well as lectin-binding techniques were used to study glycoconjugates that are present in the alimentary canal of the European eel (Anguilla anguilla). Specimens from pharynx, oesophagus, stomach and intestine were collected from adult ("silver eel" stage) females. Alcian Blue pH 2.5/PAS and High Iron Diamine/Alcian Blue pH 2.5 reactions were performed to stain neutral and acidic glycoconjugates. In addition, lectin histochemistry was applied to identify acidic glycoconjugates containing O-acylated sialic acids. Finally, the presence of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated by using biotinylated lectins. Acidic and neutral glycoconjugates were found to be secreted throughout the alimentary canal, the acidic glycoconjugates appeared to be either sialylated or sulphated. Sialylated glycoconjugates were identified to contain sialic acid substituted at carbon in position 7 (C7). Sulphated glycoconjugates were particularly abundant in the distal intestine and were not present in the secretory products of the gastric mucosa, which contained a variety of sugar residues (D-N-acetyl-galactosamine, beta-D-galactose, alpha-D-mannose, alpha-L-fucose, D-N-acetyl-glucosamine). Lectin binding was observed in mucous cells of pharynx, oesophagus and intestine, and particularly some monosaccharides (D-N-acetyl-galactosamine and beta-D-galactose) were abundantly present.
authors.affiliation: Department of Veterinary Sciences and Technologies for Food Safety, Faculty of Veterinary Medicine, University of Milan, via Trentacoste n.2, Milan 20134, Italy. cinzia.domenghini@unimi.it
authors.forename: Cinzia
authors.initials: C
authors.lastname: Domeneghini
authors.forename: Silvana
authors.initials: S
authors.lastname: Arrighi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Radaelli
authors.forename: Giampaolo
authors.initials: G
authors.lastname: Bosi
authors.forename: A
authors.initials: A
authors.lastname: Veggetti
mesh_terms: D000818:Animals; D004064:Digestive System / Q000166:cytology / Q000378:metabolism*; D004524:Eels / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism*
chemical_list: D006001:Glycoconjugates