Oligo-chip based detection of tick-borne bacteria.
We have developed an oligonucleotide-chip based assay for detection of 16S ribosomal PCR products from tick-borne bacteria. This chip contains 14 specific probes, which target variable regions of 16S rDNA of tick-borne bacteria including Borrellia spp., Rickettsia spp., Anaplasma spp., Coxiella burnetii and Francisella tularensis. The specificity of these probes was tested by hybridization of the chip with fluorescently labeled PCR products amplified from the genomic DNA of selected tick-borne bacteria. The assay was also tested for detection of tick-borne bacteria in single ticks.
Blaskovic
Dusan
D
Institute of Molecular Biology, Centre of Excellence for Molecular Medicine, Slovak Academy of Sciences, 845 51 Bratislava 45, Slovak Republic.
Barák
Imrich
I
D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001898:Borrelia / Q000235:genetics / Q000302:isolation & purification; D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis; D005604:Francisella tularensis / Q000235:genetics / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification; D017282:Tick-Borne Diseases / Q000382:microbiology*; D013987:Ticks / Q000382:microbiology*
D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S