title: Immunoexpression of androgen receptors and aromatase in testes of patient with Klinefelter's syndrome.
abstract: Klinefelter's syndrome (47, XXY) is the most common chromosome aneuploidy in men and is usually characterized by underdeveloped testes and sterility. The aim of the present study was to detect cellular distribution of androgen receptors (AR) and aromatase in testes of patient with KS. The tissue sections were processed for morphological and immunohistochemical staining. Additionally, levels of FSH, LH, PRL, estradiol, and testosterone were measured in the plasma. Morphological analysis revealed a complete absence of spermatogenesis. No germ cells were present in seminiferous tubules. In some tubules, nests of apparently degenerating Sertoli cells were found. In the interstitium, Leydig cell hyperplasia was observed. Using immunohistochemistry, nuclear AR staining was detected in Sertoli cells and peritubular cells, whereas in Leydig cells the staining was exclusively cytoplasmic. The immunostaining of aromatase was detected in the cytoplasm of Sertoli cells and Leydig cells. Increased levels of gonadotropins and decreased level of testosterone concomitantly with the cytoplasmic localization of AR in Leydig cells might contribute to the impaired testicular function in patient with KS.
authors.affiliation: Laboratory of Endocrinology and Tissue Culture, Institute of Zoology, Jagiellonian University, Cracow, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kotula-Balak
authors.forename: Leszek
authors.initials: L
authors.lastname: Bablok
authors.forename: Stanisław
authors.initials: S
authors.lastname: Fracki
authors.forename: Anna
authors.initials: A
authors.lastname: Jankowska
authors.forename: Barbara
authors.initials: B
authors.lastname: Bilińska
mesh_terms: D000328:Adult; D001141:Aromatase / Q000096:biosynthesis*; D042341:Gonadal Hormones / Q000097:blood; D006801:Humans; D007150:Immunohistochemistry; D007713:Klinefelter Syndrome / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009928:Organ Specificity; D010908:Pituitary Hormones, Anterior / Q000097:blood; D011944:Receptors, Androgen / Q000096:biosynthesis*; D013194:Staining and Labeling; D013737:Testis / Q000378:metabolism / Q000473:pathology*
chemical_list: D042341:Gonadal Hormones; D010908:Pituitary Hormones, Anterior; D011944:Receptors, Androgen; D001141:Aromatase