title: Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography.
abstract: Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.
authors.affiliation: ChangChun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Science, ChangChun, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Tsao
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Shuying
authors.initials: S
authors.lastname: Liu
authors.forename: Raymond
authors.initials: R
authors.lastname: Yang
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Young
authors.forename: Honghui
authors.initials: H
authors.lastname: Zhu
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.forename: Mingyong
authors.initials: M
authors.lastname: Xie
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Fu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification*; D013058:Mass Spectrometry; D010636:Phenols / Q000302:isolation & purification*; D010943:Plantago / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D059808:Polyphenols
chemical_list: D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols