Validation of ELISA test kits for detection of beta-agonist residues in meat.
Three ELISA test kits, the Randox ELISA beta-agonist test kit, Euro-Diagnostica test kit, and Ridascreen beta-agonist test kit, were evaluated for screening of meat and liver for beta-agonist residues in fortified and field-incurred samples. It was found that the Randox beta-agonist test kit was more suitable as a screening tool due to its accuracy, ease of use, and lower cost. The tests were able to detect beta-agonist residues at the minimum level of detection, as claimed by the suppliers. The performance of the method as assessed through recovery rates of beta-agonists in fortified samples was satisfactory with a low coefficient of variation (1-3%). Repeatability, as measured through the coefficient of correlation was also satisfactory. For field-incurred positive samples, the test kit showed a sensitivity of 100% and a low rate of false positives for goat and cow tissues. However, a high rate of apparent false positives was obtained for tissues of swine.
Ponniah
Jeyaletchumi
J
Public Health Institute, Ministry of Health, Kuala Lumpur, Malaysia. jeyakanesan@yahoo.com
Muhammad
Kharidah
K
Abdullah
Salam
S
Ganapathy
K K
KK
bt Sheikh Abdul Hamid
Nazimah
N
D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000506:poisoning; D000818:Animals; D002417:Cattle; D004350:Drug Residues / Q000032:analysis* / Q000506:poisoning; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008296:Malaysia; D008461:Meat Products / Q000032:analysis* / Q000633:toxicity; D013552:Swine; D019155:Veterinary Drugs / Q000032:analysis* / Q000506:poisoning
D000318:Adrenergic beta-Agonists; D019155:Veterinary Drugs