Cloning of duck PRL cDNA and genomic DNA.
Complementary DNA (cDNA) and genomic DNA, including flanking regions of the prolactin (PRL) gene of domesticated duck, were cloned and sequenced. Duck PRL was found to have 92.0, 91.7, and 91.4% sequence identity at the cDNA level to PRL of chicken, turkey, and quail, respectively. The predicted amino acid sequence had an overall similarity with a comparable region of chicken (93.4%), turkey (91.3%), and quail (91.3%) PRL. Mature duck PRL contains the consensus sequence for N-linked glycoslylation at position 6 which is not present in either chickens or turkeys. Thus, duck PRL is likely to be post-translationally modified differently from other avian species. Based on the cDNA sequence, the genomic structure of the gene was characterized. The duck PRL gene consists of 5 exons and 4 introns. Moreover, sequence analysis of the proximal region of duck PRL promoter revealed a high degree of similarity to that of chicken and turkey PRL promoter. These results suggest that the mechanisms, which regulate expression of the PRL gene, may be widely conserved in avian species.
Kansaku
Norio
N
Laboratory of Animal Genetics and Breeding, Azabu University, Fuchinobe, Sagamihara 229-8501, Japan. kansaku@azabu-u.ac.jp
Ohkubo
Takeshi
T
Okabayashi
Hisato
H
Guémené
Daniel
D
Kuhnlein
Urs
U
Zadworny
David
D
Shimada
Kiyoshi
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004372:Ducks / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D011388:Prolactin / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D011388:Prolactin