title: Acid hydrolysis and quantitative determination of total hexosamines of an exopolysaccharide produced by Citrobacter sp.
abstract: During the hydrolysis of an exopolysaccharide (EPS) produced by Citrobacter sp., the maximum liberation of hexosamine was obtained with 6 M HCl at 115 degrees C in an autoclave for 1 h. The glycosidic bond energy and degree of acetylation of the hexosamine in EPS were approximately 77 kJ mol(-1) and 61%, respectively. Thermal destruction of the hexosamines and the effect of salt on the hexosamine determination could be minimized under the optimized hydrolytic conditions. Using a modified Elson-Morgan method, maximum total hexosamine concentration was determined to be 3.2 g l(-1) (29% of crude EPS) after 96 h of fed-batch culture.
authors.affiliation: Department of Environmental Engineering, Graduate School of Engineering, Japan. jangjinho@yahoo.com
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Jang
authors.forename: Hui Ching
authors.initials: HC
authors.lastname: Hia
authors.forename: Michihiko
authors.initials: M
authors.lastname: Ike
authors.forename: Chiyo
authors.initials: C
authors.lastname: Inoue
authors.forename: Masanori
authors.initials: M
authors.lastname: Fujita
authors.forename: Toshiomi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000143:Acids / Q000737:chemistry*; D002954:Citrobacter / Q000378:metabolism*; D006595:Hexosamines / Q000032:analysis*; D006868:Hydrolysis; D011134:Polysaccharides / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D000143:Acids; D006595:Hexosamines; D011134:Polysaccharides