title: Guinea pig S19 ribosomal protein as precursor of C5a receptor-directed monocyte-selective leukocyte chemotactic factor.
abstract: OBJECTIVE
  To reveal the C5a receptor-mediated monocyte-selective chemoattraction of the homo-dimer of guinea pig S19 ribosomal protein (RP S19), and to study the topological relationship between the RP S19 and C5a receptor genes.
  
  
  METHODS
  cDNA cloning and nucleotide sequencing, leukocyte chemotaxis measurement, and fluorescent in situ hybridization (FISH) were performed in the guinea pig.
  
  
  RESULTS
  The amino acid sequence of the guinea pig RP S19 deduced from the cDNA nucleotide sequence was identical to the human protein. The dimer of a recombinant RP S19 attracted guinea pig monocytes but suppressed neutrophil chemotactic movement. Both effects were C5a receptor-mediated. In the FISH analysis, the signals denoting the guinea pig RP S19 gene and C5a receptor gene completely overlapped each other.
  
  
  CONCLUSIONS
  The guinea pig RP S19 dimer possessed a dual ligand effect, agonistic to the monocyte C5a receptor and antagonistic to the neutrophil receptor. The RP S19 and C5a receptor genes co-localized on the same chromosome.
authors.affiliation: Department of Molecular Pathology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan. tetsu@gpo.kumamoto-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Umeda
authors.forename: Y
authors.initials: Y
authors.lastname: Shibuya
authors.forename: U
authors.initials: U
authors.lastname: Semba
authors.forename: K
authors.initials: K
authors.lastname: Tokita
authors.forename: N
authors.initials: N
authors.lastname: Nishino
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002634:Chemotaxis, Leukocyte; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019281:Dimerization; D005260:Female; D015723:Gene Library; D006168:Guinea Pigs; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D044087:Receptor, Anaphylatoxin C5a / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D044087:Receptor, Anaphylatoxin C5a; D012269:Ribosomal Proteins; C040343:ribosomal protein S19