title: Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery.
abstract: A self-assembled nanoparticle was prepared using a hydrophobically modified glycol chitosan for gene delivery. A primary amine of glycol chitosan was modified with 5beta-cholanic acid to prepare a hydrophobically modified glycol chitosan (HGC). The modified chitosan spontaneously formed DNA nanoparticles by a hydrophobic interaction between HGC and hydrophobized DNA. As the HGC content increased, the encapsulation efficiencies of DNA increased while the size of HGC nanoparticles decreased. Upon increasing HGC contents, HGC nanoparticle became less cytotoxic. The increased HGC contents also facilitated endocytic uptakes of HGC nanoparticles by COS-1 cells, which were confirmed by a confocal microscopy. The HGC nanoparticles showed increasing in vitro transfection efficiencies in the presence serum. In vivo results also showed that the HGC nanoparticles had superior transfection efficiencies to naked DNA and a commercialized transfection agent. The HGC nanoparticles composed of hydrophobized DNA and hydrophobically modified glycol chitosan played a significant role in enhancing transfection efficiencies in vitro as well as in vivo.
authors.affiliation: Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Hawolkok-dong, Sungbuk-gu, Seoul 136-791, South Korea.
authors.forename: Hyuk Sang
authors.initials: HS
authors.lastname: Yoo
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Hesson
authors.initials: H
authors.lastname: Chung
authors.forename: Ick Chan
authors.initials: IC
authors.lastname: Kwon
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Jeong
mesh_terms: D000818:Animals; D019556:COS Cells; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D002522:Chlorocebus aethiops; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006018:Glycols / Q000008:administration & dosage / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008807:Mice, Inbred BALB C; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D006018:Glycols; D048271:Chitosan