title: Effect of recombinant human GH and GHRH on plasma metabolite levels in rainbow trout (Oncorhynchus mykiss).
abstract: The aim of the present work was to study metabolic changes in rainbow trout treated with preparations of human recombinants of growth hormone (rhGH) and GH-releasing hormone (rhGHRH), by analysing time course variations in plasma metabolite levels. Trouts were given the hormones by intraperitoneal injection in a single dose (rhGH: 0.5microg/g b.w.; rhGHRH: 0.06microg/g b.w.) or as a weekly injection over a period of 4 weeks. The concentrations of glucose, lactate, free fatty acids and glycerol were determined in plasma immediately before injection and 1, 3, 6, 12, 24, 48 and 168 hours post-injection. Results indicate that glucose and lactate levels increase, while slight variations in free fatty acids was observed after the injection of rhGH and rhGHRH.
authors.affiliation: Department of Physiology, Faculty of Biology, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Hernández Llorente
authors.forename: M J
authors.initials: MJ
authors.lastname: Dato Gómez
authors.forename: J
authors.initials: J
authors.lastname: de Costa Ruiz
authors.forename: P
authors.initials: P
authors.lastname: Mendiola López
authors.forename: S
authors.initials: S
authors.lastname: Zamora Navarro
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005990:Glycerol / Q000097:blood; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D013007:Growth Hormone-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007274:Injections, Intraperitoneal; D007773:Lactates / Q000097:blood; D017686:Oncorhynchus mykiss / Q000097:blood*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D011994:Recombinant Proteins; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D005990:Glycerol