How growth affects the fate of cellular metabolites.
Cellular metabolites frequently have more than a single function in the cell. For example they may be sources of energy as well as building blocks for several macromolecules. The relative cellular needs for these different functions depend on environmental and intracellular factors. The intermediary products of phosphorylation of pyruvate by mitochondria, for example, are used for growth, while the released ATP is used for both growth and maintenance. Since maintenance has priority over growth, and maintenance is proportional to a cell's mass, a cell's need for ATP vs. building blocks depends on the growth rate, and hence on substrate availability. We show how the concept of Synthesising Units (SUs) in linear and cyclic pathways takes care of the correct variation of the ATP/building block ratio in the context of the Dynamic Energy Budget (DEB) theory. This can only be achieved by an interaction between subsequent SUs in transferring metabolites. Apart from this interaction we also needed an essential feature of the performance of the pathway in the DEB context: the relative amount of enzymes varies with the growth rate in a special way. We solved an important consistency problem between the DEB model at the whole-cell level and a model for pathway dynamics. We observe that alternative whole-cell models, such as the Marr-Pirt model, that keep the relative amount of enzymes constant, and hence independent of the growth rate, will have problems in explaining how pathways can meet cells' growth-dependent needs for building blocks vs. ATP.
Kooijman
S A L M
SA
Department of Theoretical Biology, Faculty of Earth and Life Sciences, Vrije Universiteit, de Boelelaan 1087, 1081 HV Amsterdam, Netherlands. bas@bio.vu.nl
Segel
L A
LA
D000255:Adenosine Triphosphate / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D002455:Cell Division; D003198:Computer Simulation; D004734:Energy Metabolism / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D012984:Software
D000255:Adenosine Triphosphate