Preparation of gold colloid monolayer by immunological identification.
The design and initial characterization of the self-assembled gold colloid monolayer by a sandwich structure via the immunological identification are reported. The 13 nm gold colloid nanoparticles and the silicon or quartz substrates have been modified with the mouse polyclonal antibody against hepatitis B virus surface antigen (PAb) and the mouse monoclonal antibody against hepatitis B virus surface antigen (MAb), respectively. They can be linked by a special reaction with their corresponding hepatitis B virus surface antigen (Antigen) as a sandwich structure. Thus, the density of gold nanoparticles self-assembled on the substrate can be readily controlled by the amount of the antigen added. The resulting substrates have been characterized by atomic force microscopy (AFM) and surface-enhanced Raman scattering (SERS) spectroscopy when the gold nanoparticles were modified with SERS-active probe molecules of 4-mercaptobenzoic acid (MBA) after silver enhancement. These data show that the gold nanoparticles are separately fixed onto the substrate and form a uniform monolayer, which possess a set of features that make them very attractive for both basic and applied uses, including roughness, high stability, and biocompatibility.
Xu
Weiqing
W
Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, PR China.
Xu
Shuping
S
Ji
Xiaohui
X
Song
Bo
B
Yuan
Hang
H
Ma
Lan
L
Bai
Yubai
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001565:Benzoates / Q000737:chemistry; D017979:Gold Colloid / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D051379:Mice; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D012834:Silver; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds
D000911:Antibodies, Monoclonal; D001565:Benzoates; D017979:Gold Colloid; D006514:Hepatitis B Surface Antigens; D013438:Sulfhydryl Compounds; C013594:4-mercaptobenzoate; D012834:Silver