[Determination of flavoniod-glycosides in Yixintong pills by HPLC].
OBJECTIVE
To develop an HPLC method for determining flavoniod-glycosides in Yixintong pills.


METHODS
Agilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30:3:4:120:3) as mobile phase. Detection wavelength was 330 nm.


RESULTS
Good linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively.


CONCLUSIONS
This method can be used for quality control of Yixintong pills.
Su
Juan
J
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Zhang
Wei-dong
WD
Zhou
Yun
Y
Zhou
Jing
J
Gu
Zheng-bing
ZB
D047310:Apigenin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D004365:Drugs, Chinese Herbal; C021329:vitexin rhamnoside; C058773:glucosylvitexin; D047310:Apigenin