title: Kinetic modelling of continuous submerged fermentation of cheese whey for single cell protein production.
abstract: A mathematical model describing the kinetics of continuous production of single cell protein from cheese whey using Kluyveromyces fragilis was developed from the basic principles of mass balance. The model takes into account the substrate utilization for growth and maintenance and the effect of substrate concentration and cell death rate on the net cell growth and substrate utilization during the fermentation process. A lactose concentration below 1.91 g/L limited growth of yeast cells whereas a lactose concentration above 75 g/L inhibited the growth of the yeast. The model was tested using experimental data obtained from a continuous system operated at various retention times (12, 18 and 24 h), mixing speeds (200, 400 and 600 rpm) and air flow rates (1 and 3 vvm). The model was capable of predicting the effluent cell and substrate concentrations with R2 ranging from 0.95 to 0.99. The viable cell mass and lactose consumption ranged from 1.3 to 34.3 g/L and from 74.31% to 99.02%, respectively. A cell yield of 0.74 g cell/g lactose (close to the stoichiometric value of 0.79 g cell/g lactose) was achieved at the 12 h retention time-3 vvm air flow rate-600 rpm mixing speed combination. The total biomass output (viable and dead cells) at this combination was 37 g/L.
authors.affiliation: Department of Biological Engineering, Dalhousie University, P.O. Box 1000, Halifax, Nova Scotia, Canada B3J 2X4. abdel.ghaly@dal.ca
authors.forename: A E
authors.initials: AE
authors.lastname: Ghaly
authors.forename: M
authors.initials: M
authors.lastname: Kamal
authors.forename: L R
authors.initials: LR
authors.lastname: Correia
mesh_terms: D018533:Biomass; D019149:Bioreactors; D004044:Dietary Proteins / Q000378:metabolism*; D007700:Kinetics; D007716:Kluyveromyces / Q000378:metabolism*; D007785:Lactose / Q000096:biosynthesis; D008894:Milk Proteins / Q000378:metabolism*; D008954:Models, Biological; D000067816:Whey Proteins
chemical_list: D004044:Dietary Proteins; D008894:Milk Proteins; D000067816:Whey Proteins; C012551:single cell proteins; D007785:Lactose