Subcellular imaging mass spectrometry of brain tissue.
Imaging mass spectrometry provides both chemical information and the spatial distribution of each analyte detected. Here it is demonstrated how imaging mass spectrometry of tissue at subcellular resolution can be achieved by combining the high spatial resolution of secondary ion mass spectrometry (SIMS) with the sample preparation protocols of matrix-assisted laser desorption/ionization (MALDI). Despite mechanistic differences and sampling 10(5) times less material, matrix-enhanced (ME)-SIMS of tissue samples yields similar results to MALDI (up to m/z 2500), in agreement with previous studies on standard compounds. In this regard ME-SIMS represents an attractive alternative to polyatomic primary ions for increasing the molecular ion yield. ME-SIMS of whole organs and thin sections of the cerebral ganglia of Lymnaea stagnalis demonstrate the advantages of ME-SIMS for chemical imaging mass spectrometry. Subcellular distributions of cellular analytes are clearly obtained, and the matrix provides an in situ height map of the tissue, allowing the user to identify rapidly regions prone to topographical artifacts and to deconvolute topographical losses in mass resolution and signal-to-noise ratio.
McDonnell
Liam A
LA
FOM Institute for Atomic and Molecular Physics, Kruislaan 407, 1098 SJ Amsterdam, The Netherlands.
Piersma
Sander R
SR
MaartenAltelaar
A F
AF
Mize
Todd H
TH
Luxembourg
Stefan L
SL
Verhaert
Peter D E M
PD
van Minnen
Jan
J
Heeren
Ron M A
RM
D000818:Animals; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D002784:Cholesterol / Q000032:analysis; D003058:Cockroaches / Q000737:chemistry / Q000166:cytology*; D005841:Gentisates; D008195:Lymnaea / Q000737:chemistry / Q000166:cytology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods*; D014269:Trifluoroacetic Acid
D005841:Gentisates; D002784:Cholesterol; D014269:Trifluoroacetic Acid; C010925:2,5-dihydroxybenzoic acid