title: A comparison of methods employed to evaluate antioxidant capabilities.
abstract: Three different methodologies frequently employed to evaluate the indexes that report the antioxidant capabilities of pure compounds and/or complex mixtures of antioxidants are applied to a series of mono- and polyphenols, as well as to two wine (red and white) samples. These methodologies are based on the bleaching of a stable radical, the effect of the additive upon luminol chemiluminescence induced by peroxyl radicals, and the effect of the additive upon the bleaching of the fluorescence from a dye molecule. Widely different responses are obtained from the different methodologies. These differences are interpreted in terms of the different factors (stoichiometric factors and/or reactivities) that determines the indexes evaluated by these different methodologies.
authors.affiliation: Faculty of Biological Sciences, Catholic University of Chile, Santiago.
authors.forename: D D
authors.initials: DD
authors.lastname: Perez
authors.forename: F
authors.initials: F
authors.lastname: Leighton
authors.forename: A
authors.initials: A
authors.lastname: Aspee
authors.forename: C
authors.initials: C
authors.lastname: Aliaga
authors.forename: E
authors.initials: E
authors.lastname: Lissi
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002839:Chromans / Q000737:chemistry*; D005419:Flavonoids; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol; D010545:Peroxides / Q000737:chemistry*; D010636:Phenols; D059808:Polyphenols; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D002839:Chromans; D005419:Flavonoids; D010545:Peroxides; D010636:Phenols; D059808:Polyphenols; C049375:perhydroxyl radical; D008165:Luminol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid