title: [Effects of combined use of curcumin and catechin on cyclooxygenase-2 mRNA expression in dimethylhydrazine-induced rat colon carcinogenesis].
abstract: OBJECTIVE
  To examine the effect of combined use of curcumin and catechin on the number of aberrant crypt foci (ACF) and expression levels of cyclooxygenase-2 (COX-2) mRNA in rat colon carcinogenesis. Methods Dimethylhydrazine (DMH)-induced rats colon carcinogenesis model was used for evaluation of the synergistic inhibitory effect between curcumin and catechin in light of ACF formation and tumor incidence. COX-2 mRNA expression was also detected in rat colon carcinogenesis.
  
  
  RESULTS
  Curcumin, catechin and their co-treatment caused significant inhibition of DMH-induced ACF and colon carcinogenesis as compared with untreated DMH-induced rat models (P<0.01). Co-treatment with curcumin and catechins caused greater inhibition of DMH-induced ACF and colon carcinogenesis than the single use of curcumin or catechin (P<0.05). A synergistic inhibitory effect between curcumin and catechin on the expression of COX-2 mRNA was observed in the early stage of rat colon carcinogenesis but not in colon tumor tissues.
  
  
  CONCLUSIONS
  Curcumin and catechin have synergistic effect on ACF and COX-2 mRNA expression in rat colon carcinogenesis, suggesting their potential value in the prevention of human colon cancers.
authors.affiliation: Institute for Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xgvip@tom.com
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Zhang
authors.forename: Zhuo-sheng
authors.initials: ZS
authors.lastname: Lai
authors.forename: Mei-rong
authors.initials: MR
authors.lastname: He
authors.forename: Ya-dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Zhang
mesh_terms: D019813:1,2-Dimethylhydrazine; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002273:Carcinogens; D002392:Catechin / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D004357:Drug Synergism; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002273:Carcinogens; D012333:RNA, Messenger; D002392:Catechin; D051546:Cyclooxygenase 2; D003474:Curcumin; D019813:1,2-Dimethylhydrazine