title: Comparison of the complete genome sequence between C1 and G4 isolates of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.
abstract: The complete nucleotide sequence of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus isolate C1 (HearSNPV-C1) was determined and analyzed by comparing with the genome of HearSNPV-G4 isolate. C1 and G4 isolates occurred in the same host species and geographic location but showed different virulence. The HearSNPV-C1 genome consisted of 130,759 bp and 137 putative open reading frames larger than 150 nucleotides were identified. The two genomes shared 98.1% nucleotide sequence identity, with a total number of 555 bp substitutions, 1354 bp deletions, and 710 bp insertions in HearSNPV-C1. Comparison of ORFs and homologous repeat (hr) regions of the two genomes showed that there were four highly variable regions hr1, hr4, hr5, and bro-b, all in repeat regions. These results suggest that baculovirus strain heterogeneity may be often caused by SNPs and changes in the hrs and bro genes.
authors.affiliation: Institute of Applied Entomology, Zhejiang University, Kaixuan Road 268, Hangzhou, Zhejiang 310029, China. chxzhang@zju.edu.cn
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
authors.forename: Xiu-Cui
authors.initials: XC
authors.lastname: Ma
authors.forename: Zhong-Jian
authors.initials: ZJ
authors.lastname: Guo
mesh_terms: D000818:Animals; D001483:Base Sequence; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000472:pathogenicity; D016366:Open Reading Frames; D020641:Polymorphism, Single Nucleotide; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence / Q000235:genetics