[Studies on mechanism of antiasthmatic and anti-inflammatory actions of CDCA].
OBJECTIVE
To observe antiasthmatic and anti-inflammatory actions mechanisms of CDCA.


METHODS
The content of NO was determined by method of nitroreductase chromatometry in serum and trachea tissue. The content of cAMP was analysed by method of competitive protein-binding assay. The content of PGE2 was determined by method of ultraviolet spectrophotometry.


RESULTS
CDCA significantly decreased the content of NO of serum and trachea tissue in mice. CDCA increased greatly the content of cAMP of trachea tissue in rats. CDCA significantly decreased the content of PGE2 of trachea and lung tissue in mice.


CONCLUSIONS
Mechanisms of antiasthmatic and anti-inflammatory actions of CDCA are related to increasing the content of cAMP in trachea tissue and decreasing the constituent of NO and PGE2 in body.
Li
Pei-feng
PF
The College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhhot 010018, China. lpeifeng@imau.edu.cn
Guan
Hong
H
Zhao
Hong-xia
HX
Si
Qin
Q
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002635:Chenodeoxycholic Acid / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D008168:Lung / Q000378:metabolism; D008297:Male; D008419:Materia Medica / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000378:metabolism
D018927:Anti-Asthmatic Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D008419:Materia Medica; D002635:Chenodeoxycholic Acid; D009569:Nitric Oxide; D000242:Cyclic AMP; D015232:Dinoprostone