title: Sequential application of interphase-FISH and CGH to single cells.
abstract: A comprehensive genomic analysis of single cells is needed for numerous scenarios in tumor genetics, clinical diagnostics and forensic application. PCR protocols were developed which allow an unbiased amplification of the whole genome of a single cell for subsequent analyses by comparative genomic hybridization (CGH). However, verification of single-cell CGH results has been impossible as the procedure naturally involves the destruction of the respective cell. Here we show that the genome of individual cells can be analyzed by two different single cell techniques applied sequentially to the same cell. In a first step, interphase fluorescence in situ hybridization (FISH) is applied. After evaluation of the interphase-FISH signals, cells of interest can be selected for a further analysis. Single cells are collected by laser microdissection, the DNA is amplified by linker-adaptor PCR and subjected to CGH-analysis. This strategy offers new opportunities for a sophisticated selection of cells based on interphase-FISH signals. Furthermore, the sequential application of two different single-cell approaches to the same single-cell represents the only option to control and verify the single-cell CGH results. We demonstrate the feasibility of this approach with a series of experiments including cells from pre- and postnatal diagnostics, for example, cells with trisomies 13, 18, or 21, respectively, leukemia and tumor cells and tissue sections.
authors.affiliation: Institut für Humangenetik, Technische Universität München, Munich, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Langer
authors.forename: Jochen B
authors.initials: JB
authors.lastname: Geigl
authors.forename: Rainer
authors.initials: R
authors.lastname: Gangnus
authors.forename: Michael R
authors.initials: MR
authors.lastname: Speicher
mesh_terms: D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D009693:Nucleic Acid Hybridization