Penalized binary regression for gene expression profiling.
OBJECTIVE
A typical bioinformatics task in microarray analysis is the classification of biological samples into two alternative categories. A procedure is needed which, based on the expression levels measured, allows us to compute the probability that a new sample belongs to a certain class.


METHODS
For the purpose of classification the statistical approach of binary regression is considered. Highdimensionality and at the same time small sample sizes make it a challenging task. Standard logit or probit regression fails because of condition problems and poor predictive performance. The concepts of frequentist and of Bayesian penalization for binary regression are introduced. A Bayesian interpretation of the penalized log-likelihood is given. Finally the role of cross-validation for regularization and feature selection is discussed.


RESULTS
Penalization makes classical binary regression a suitable tool for microarray analysis. We illustrate penalized logit and Bayesian probit regression on a well-known data set and compare the obtained results, also with respect to published results from decision trees.


CONCLUSIONS
The frequentist and the Bayesian penalization concept work equally well on the example data, however some method-specific differences can be made out. Moreover the Bayesian approach yields a quantification (posterior probabilities) of the bias due to the constraining assumptions.
Schimek
Michael G
MG
Medical University of Graz, Institute for Medical Informatics, Statistics and Documentation, Auenbruggerplatz 2, 8036 Graz, Austria. michael.schimek@meduni-graz.at
D001499:Bayes Theorem; D019295:Computational Biology; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis / Q000145:classification* / Q000706:statistics & numerical data