Cytokine responses induced by Mycobacterium tuberculosis in patients with HIV-1 infection and tuberculosis.
OBJECTIVE
Tuberculosis (TB) is an important opportunistic infection in HIV patients. Immune responses to Mycobacterium tuberculosis in HIV/TB patients were evaluated.


METHODS
Fifteen patients with HIV/TB, ten with HIV, four with TB, and five controls were enrolled. Peripheral blood mononuclear cells were isolated and stimulated with mycobacterial antigen (PPD). Interferon (IFN)-gamma and TNF-alpha in culture supernatants were measured by ELISA.


RESULTS
IFN-gamma and TNF-alpha production after PPD stimulation was markedly decreased in HIV patients, but not in HIV/TB patients. In HIV patients with a CD4 cell count of less than 200/mm3, IFN-gamma and TNF-alpha production after PPD stimulation was higher in HIV/TB patients than in HIV patients. Cytokine responses to M. tuberculosis reconstituted after highly active antiretroviral therapy (HAART) and were prominent in HIV/TB patients.


CONCLUSIONS
Cytokine responses to M. tuberculosis were retained in HIV-infected patients with tuberculosis, even in patients with a CD4 cell count of less than 200/mm3, and reconstituted after HAART.
Oh
Myoung-Don
MD
Department of Internal Medicine, Seoul National University College of Medicine, Seoul, 110-744, Republic of Korea.
Kang
Cheol-In
CI
Kim
Ui-Seok
US
Kim
Nam Joong
NJ
Lee
Bobin
B
Kim
Hong Bin
HB
Choe
Kang Won
KW
D017088:AIDS-Related Opportunistic Infections; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial; D023241:Antiretroviral Therapy, Highly Active; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D014376:Tuberculosis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000942:Antigens, Bacterial; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma