Titania-coated monolithic silica as separation medium for high performance liquid chromatography of phosphorus-containing compounds.
A method of preparing titania-coated monolithic silica stationary phase has been developed to achieve liquid chromatographic separation of phosphorus-containing compounds, which have recently been attracting increasing attention in biochemical research. The titania-coated silica columns exhibited efficient separation with low pressure drop, which is a typical feature of monolithic structures, and also possessed phospho-selectivity, which is a unique property of the titania surface. The material characteristics of titania-coated monolithic silica were examined, and then resin-clad columns were applied to the HPLC analysis of phosphorylated compounds. Highly efficient separation of phosphorylated substances indicated that the novel titania-coated monolithic silica column will find applications as a useful tool in the field of biochemistry, especially in post-genomic analyses.
Miyazaki
Shota
S
GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama 358-0032, Japan. s-miyazaki@gls.co.jp
Miah
Muhammed Yusuf
MY
Morisato
Kei
K
Shintani
Yukihiro
Y
Kuroha
Tomohiro
T
Nakanishi
Kazuki
K