title: Frequent loss of RUNX3 gene expression in remnant stomach cancer and adjacent mucosa with special reference to topography.
abstract: Our previous studies suggest that a lack of RUNX3 function is causally related to the genesis and progression of human gastric cancer. This study was conducted to determine whether alteration of RUNX3 gene expression could be detected in the normal-looking gastric remnant mucosa, and to ascertain any difference in the potential of gastric carcinogenesis between the anastomotic site and other areas in the remnant stomach after distal gastrectomy for peptic ulcer (RB group) or gastric cancer (RM group), by analysing RUNX3 expression with special reference to topography. A total of 89 patients underwent distal gastrectomy for gastric cancer from the intact stomach (GCI group) and 58 patients underwent resection of the remnant stomach for gastric cancer (RB group: 34 cases, RM group: 24 cases). We detected RUNX3 and gene promoter methylation by in situ hybridisation, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), and methylation-specific PCR. The interval between the initial surgery and surgery for remnant gastric cancer (interval time) was 10.4 years in the RM group, and 27.5 years in the RB group. Cancers in the RB group were significantly more predominant in the anastomosis area (P<0.05). Within the tumour, downregulation of RUNX3 expression ranged from 74.7 to 85.7% in the three groups. The rate of downregulation of RUNX3 of adjacent mucosa was 39.2% (11 in 28 cases) in RB and 47.6% (10 in 21 cases) in RM, which are significantly higher than that of the GCI group (19.5%, 17 in 87 cases). In noncancerous mucosa of the remnant stomach in the RB group, RUNX3 expression decreased more near the anastomosis area. In the RM group, however, there were no significant differences in RUNX3 expression by sampling location. Based on RUNX3 downregulation and clinical features, residual stomach mucosa of the RM group would have a higher potential of gastric carcinogenesis compared to the RB or GCI group. Gastric stump mucosa of the RB group has higher potential especially than other areas of residual stomach mucosa. Measurement of RUNX3 expression and detection of RUNX3 methylation in remnant gastric mucosa may estimate the forward risk of carcinogenesis in the remnant stomach.
authors.affiliation: Department of Surgery and Physiology of Digestive System, Graduate School of Medical Science, Surgery and Regenerative Medicine, Kyoto, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakase
authors.forename: C
authors.initials: C
authors.lastname: Sakakura
authors.forename: K
authors.initials: K
authors.lastname: Miyagawa
authors.forename: S
authors.initials: S
authors.lastname: Kin
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
authors.forename: A
authors.initials: A
authors.lastname: Yanagisawa
authors.forename: K
authors.initials: K
authors.lastname: Koide
authors.forename: N
authors.initials: N
authors.lastname: Morofuji
authors.forename: Y
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: K
authors.initials: K
authors.lastname: Shimomura
authors.forename: K
authors.initials: K
authors.lastname: Katsura
authors.forename: A
authors.initials: A
authors.lastname: Hagiwara
authors.forename: H
authors.initials: H
authors.lastname: Yamagishi
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000368:Aged; D001483:Base Sequence; D050677:Core Binding Factor Alpha 3 Subunit; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D018530:Gastric Stump / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008745:Methylation; D008875:Middle Aged; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013274:Stomach Neoplasms / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; D004268:DNA-Binding Proteins; C493729:Runx3 protein, human; D014157:Transcription Factors