A chimeric Plasmodium falciparum Pfnbp2b/Pfnbp2a gene originated during asexual growth.
The Plasmodium falciparum line 3D7-A has an unusual invasion phenotype, such that it can invade enzyme-treated and mutant red blood cells that are resistant to invasion by other parasite lines. 3D7-A has a chimeric Pfnbp2b gene that contains part of the repeat region of the paralogous gene Pfnbp2a. This chimeric gene originated by spontaneous gene conversion during normal maintenance in culture, indicating that ectopic recombination and gene conversion during asexual growth are potentially important mechanisms participating in the evolution of paralogous genes in Plasmodium. However, the presence of the chimeric Pfnbp2b gene in 3D7-A was not associated with its peculiar invasion phenotype.
Cortés
Alfred
A
Molecular Parasitology Laboratory, Papua New Guinea Institute of Medical Research, Madang, P.O. Box 378, MP511, Papua New Guinea. acortes@nimr.mrc.ac.uk
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D004912:Erythrocytes / Q000469:parasitology; D005785:Gene Conversion / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics*; D006801:Humans; D016778:Malaria, Falciparum / Q000235:genetics / Q000635:transmission; D008969:Molecular Sequence Data; D010641:Phenotype; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D000913:Antibodies, Protozoan; C502039:Nbp2a protein, Plasmodium falciparum; C502038:Nbp2b protein, Plasmodium falciparum; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins