Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate.
Hoki (Johnius belengerii) skin gelatin was hydrolyzed with three commercial enzymes to identify radical-scavenging potencies of derived peptides. Peptides derived from tryptic hydrolysate exhibited the highest scavenging activities on superoxide, carbon-centered 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals assessed by ESR spectroscopy. Following consecutive chromatographic separations of tryptic hydroolysate, the peptide sequence His-Gly-Pro-Leu-Gly-Pro-Leu (797 Da) acted as a strong radical scavenger under studied conditions. Further, this peptide could act as an antioxidant against linoleic acid peroxidation and the activity was closer to the highly active synthetic antioxidant butylated hydroxytoluene (BHT). In addition, antioxidative enzyme levels in cultured human hepatoma cells were increased in the presence of this peptide and it was presumed to be the peptide involved in maintaining the redox balance in the cell environment. Present data indicate that free-radical-scavenging activities of hoki skin gelatin peptides substantially contribute to their antioxidant properties measured in different oxidative systems.
Mendis
Eresha
E
Department of Chemistry, Pukyong National University, Busan 608-737, South Korea.
Rajapakse
Niranjan
N
Kim
Se-Kwon
SK
D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular; D005399:Fishes; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D019787:Linoleic Acid / Q000737:chemistry; D008113:Liver Neoplasms; D010084:Oxidation-Reduction; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D016166:Free Radical Scavengers; D010455:Peptides; D005780:Gelatin; D019787:Linoleic Acid