title: [Studies on tissue culture of Dendrobium lituiflorum].
abstract: OBJECTIVE
  To select optimal media and conditions for the tissue culture of Dendrobium lituiforum.
  
  
  METHODS
  The basic media, light illumination condition, phytohormone, and natural aqueous extracts. were tested and compared. The chlorophyll contents and soluble protein contents were measured.
  
  
  RESULTS
  N6 medium was suitable for embryo germination and growth. 1/2 MS + NAA 0.5 mg x L(-1) cordd be used as the optimal protocorm multiplying media. Culture 20 days in darkness in advance, and then in light is useful to embryo germinate. 1/2 MS+ NAA 0-0.5 mg x L(-1) is beneficial to protocorm multiplication largely. N6 + 6-BA 2.0 mg x L(-1) + NAA 0.5 mg x L(-1) + 10% potato juice is useful to protocorm differentiation. Phytohormone and potato juice added to the media promoted chlorophyll content and souble protein content. N6 + NAA 0.5 mg x L(-1) + 10% banana juice is the best medium for plantlet rootage and strengthening.
  
  
  CONCLUSIONS
  Concentration of inorganic salt, nitrogen source and illumination condition are important to embryo germination and growth. The nitrogen source type has effect on the protocorm multiplication. The chlorophyll contents and souble protein contents may be index to indicate the growth condition of plantlets, which can help to select the optimal media.
authors.affiliation: School of Life Science, Nanjing University, Nanjing Normal 210097, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Ding
authors.forename: Shu-ling
authors.initials: SL
authors.lastname: Bao
authors.forename: Dong-yang
authors.initials: DY
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Tang
authors.forename: Bing-zhong
authors.initials: BZ
authors.lastname: Ding
mesh_terms: D002734:Chlorophyll / Q000032:analysis*; D003470:Culture Media; D031667:Dendrobium / Q000737:chemistry / Q000254:growth & development*; D008027:Light; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development*; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D010940:Plant Proteins; D002734:Chlorophyll