Quantitative determination of the major saponin mixture bacoside A in Bacopa monnieri by HPLC.
Bacoside A, the putative bioactive component of the Indian medicinal plant Bacopa monnieri, was found to be a mixture of saponins with bacoside A3 (1), bacopaside II (2), jujubogenin isomer of bacopasaponin C (3) and bacopasaponin C (4) as major constituents. An HPLC method together with an optimised extraction procedure was developed for the estimation of 1-4 in B. monnieri to enable standardisation of the latter. Concentration ranges of the analytes in samples of B. monnieri collected from different regions of India were 0.14-0.85% (w/w) (1), 0.12-0.69% (2), 0.05-0.72% (3) and 0.05-0.44% (4). The importance of using bacoside A, with known concentrations of 1-4, as a reference standard for the routine analysis of B. monnieri is highlighted. Two common flavonoids, luteolin and apigenin, were present in all samples of B. monnieri.
Deepak
M
M
Department of Phytochemistry, Research and Development Centre, Natural Remedies Pvt Ltd, Veerasandra Industrial Area, Bangalore-560 100, India. phytochemistry@naturalremedy.com
Sangli
G K
GK
Arun
P C
PC
Amit
A
A
D034681:Bacopa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
D012503:Saponins; D014315:Triterpenes; C500175:bacoside A