Fibroin-modulator-binding protein-1 (FMBP-1) contains a novel DNA-binding domain, repeats of the score and three amino acid peptide (STP), conserved from Caenorhabditis elegans to humans.
The predicted transcriptional regulatory factor for the fibroin gene of the silkworm Bombyx mori, fibroin-modulator-binding protein-1 (FMBP-1), was purified by sequential DNA affinity column chromatography, and cDNA clones corresponding to FMBP-1 were isolated from a library. The N-terminal half of FMBP-1 has a weak similarity to the DNA-binding domain of several transcriptional regulatory factors in higher plants. The C-terminal half contains four tandem repeats of a novel 23 amino acid motif, which we named the score and three amino acid peptide (STP). Other genes containing STP repeats were found in Drosophila, Caenorhabditis elegans, mouse and human. Mutational analysis of FMBP-1 showed that the STP repeats form a novel DNA-binding domain. Sequences flanking STP repeats modulated DNA-binding activity. The FMBP-1 gene was expressed during the fourth to fifth instar. FMBP-1 activity appeared to be regulated at the transcriptional level and by the post-transcriptional modification.
Takiya
Shigeharu
S
Center for Advanced Science and Technology and Hokkaido University North 10, West 8, Kita-ku, Sapporo 060-0810, Japan.
Ishikawa
Tetsurou
T
Ohtsuka
Katsuya
K
Nishita
Yoshinori
Y
Suzuki
Yoshiaki
Y
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000235:genetics; D002846:Chromatography, Affinity; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D005349:Fibroins / Q000235:genetics; D017344:Genes, Insect; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D019476:Insect Proteins; D005349:Fibroins