Growth dynamics of major microbial populations during biodegradation of o-phthalate in anaerobic sediment slurries under a CO2/H2 atmosphere.
o-Phthalate transformers increased about five orders of magnitude (to 1.6x10(11)cells g-1 sediment) just before the onset of fast biotransformation of o-phthalate (21.6 mg l-1) and then decreased sharply when the concentration of o-phthalate became low during biodegradation of o-phthalate in anaerobic sediment slurries under CO2/H2 (4:1, v/v). In contrast, the benzoate transformers increased about four orders of magnitude (to 1.6x10(11)cells g-1 sediment) in 48 days and then increased one more order (to 1.6x10(12)cells g-1 sediment) in 60 days and then remained at that high level in those sediment slurries. When making a comparison between the growth dynamics of o-phthalate transformers, acetogens, sulfate reducers, and methanogens and the time course of o-phthalate transformation, it appears that acetogens did not initiate biotransformation of o-phthalate, and that sulfate reducers and methanogens were not directly involved in o-phthalte degradation. o-Phthalate was not transformed in sediment slurries amended with BESA plus molybdate under CO2/H2.
Liu
S-M
SM
Institute of Marine Biology, National Taiwan Ocean University, 2-Pening Road, Keelung, Taiwan. smliu@mail.ntou.edu.tw
Lin
Y-L
YL
Tsai
T-L
TL
D001421:Bacteria, Anaerobic / Q000254:growth & development* / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism / Q000494:pharmacology; D001673:Biodegradation, Environmental; D019149:Bioreactors; D001711:Biotransformation / Q000187:drug effects; D002245:Carbon Dioxide; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D019015:Geologic Sediments / Q000382:microbiology*; D006859:Hydrogen; D008697:Methane / Q000378:metabolism; D008982:Molybdenum / Q000633:toxicity; D010795:Phthalic Acids / Q000378:metabolism*; D013624:Taiwan; D013997:Time Factors
D001565:Benzoates; D010795:Phthalic Acids; C044659:molybdate; D002245:Carbon Dioxide; C032279:phthalic acid; D006859:Hydrogen; D008982:Molybdenum; D008697:Methane