[Effect of cytostatic therapy on IL-1beta and L-selectin concentrations, synthesis, accumulation and excretion in patients with acute myeloid leukaemia].
The etiology and pathogenesis of the majority of diseases that make up the acute leukemias is unknown. A change in IL-1beta and L selectin concentrations is most likely to occur in the course of subtype M2 of acute myeloid leukaemia (AML). The purpose of our study was to examine the change in concentrations of these molecules mentioned above in blood plasma, culture supernatant and isolated, broken granulocytes in AML patients in both exacerbation and remission of the disease and in healthy control group. Cytokine concentration assay was performed by means of ready immunoenzymatic sets of ELISA type. The examinations were carried out in leukaemic leukocyte cultures Neupogen--stimulated or nonstimulated. Mitogen was added to activate granulocytes and to provoke blastic transformation. A significant increase in IL-1beta concentration was found in AML--exacerbation and remission of the disease in blood plasma, culture supernatant and isolated, broken granulocytes. In all cases L-selectin concentrations were increased in exacerbation and decrease in remission of AML after typical chemotherapy in comparison to controls. A significant increase between the concentrations of cytokines were observed in cultures Neupogen--stimulated and non-stimulated.
Kiersnowska-Rogowska
Beata
B
Klinika Hematologii Akademii Medycznej w Białymstoku.
Parfieńczyk
Adam
A
Rogowski
Franciszek
F
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D019041:L-Selectin / Q000187:drug effects* / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011044:Poland
D000970:Antineoplastic Agents; D007375:Interleukin-1; D019041:L-Selectin