Proteomics of bovine mitochondrial RNA-binding proteins: HES1/KNP-I is a new mitochondrial resident protein.
Proteomic analysis of bovine mitochondrial proteins with affinity to polyAdenylate or polyUridylate was performed in an effort to identify novel RNA-binding mitochondrial proteins. We have used 2D gel electrophoresis and MALDI-QqTOF mass spectrometry to identify a total of 64 proteins, of which 51 have defined mitochondrial function including 6 known RNA-binding proteins. HES1/KNP-I from the polyA-binding fraction of mitochondrial Triton extract showed exclusive mitochondrial localization when expressed in GFP-tagged form. The HES1/KNP-I gene is on human chromosome 21q22.3 and may be involved in several disorders mapped to that region. Thus, HES1/KNP-I is a proven mitochondrial resident protein with apparent tight membrane association and tentative RNA-binding properties.
Ponamarev
Mikhail V
MV
Department of Metabolism and Department of Structural Biology and Biochemistry, Hospital for Sick Children Research Institute, Toronto M5G 1X8, Ontario, Canada.
She
Yi-Min
YM
Zhang
Li
L
Robinson
Brian H
BH
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D024101:Mitochondrial Proteins / Q000032:analysis / Q000302:isolation & purification*; D017830:Octoxynol; D011061:Poly A; D011072:Poly U; D040901:Proteomics / Q000379:methods*; D016601:RNA-Binding Proteins / Q000032:analysis / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D024101:Mitochondrial Proteins; D016601:RNA-Binding Proteins; D011061:Poly A; D011072:Poly U; D017830:Octoxynol