title: Development of a high-resolution magic-angle spinning nuclear magnetic resonance identity assay of the capsular polysaccharide from Haemophilus influenzae type b present in cetavlon precipitate.
abstract: We describe the use of high-resolution magic-angle spinning nuclear magnetic resonance to control the identity of the capsular polysaccharide from Haemophilus influenzae type b (Hib) present in the cetavlon precipitate. This step is one of the earliest in the purification of this polysaccharide, which is further used in the production of Hib polysaccharide-protein conjugate vaccine. The effects of sample procedure and magnetic field strength have been investigated. Since this assay is rapid and simple, it may represent a useful technique for characterization of polysaccharides present in complex and insoluble matrices. Moreover, it allows a rapid evaluation of the structure of the produced polysaccharides very early on during the production process and is as such an essential analytical tool before starting the purification process.
authors.affiliation: CNRS/Université de Lille 2, UMR 8525, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: Philippe
authors.initials: P
authors.lastname: Talaga
authors.forename: Guy
authors.initials: G
authors.lastname: Lippens
mesh_terms: D016667:Bacterial Capsules; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D011232:Chemical Precipitation; D018073:Haemophilus Vaccines / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D002593:Cetrimonium Compounds; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial; D000077286:Cetrimonium