title: A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel.
abstract: A-type K(+) channels belonging to the Shal subfamily are found in various receptor and neuronal cells. Although their kinetics and cell surface expression are regulated by auxiliary subunits, little is known about the proteins that may interact with Kv4 during development. A yeast two-hybrid screening of a cDNA library made from the sensory epithelium of embryonic chick cochlea revealed a novel association of Kv4.2 with a protein containing a pentraxin domain (PPTX). Sequence analysis shows that PPTX is a member of the long pentraxin family, is 53% identical to mouse PTX3, and has a signal peptide at the N terminus. Studies with chick cochlear tissues reveal that Kv4.2 coprecipitates PPTX and that both proteins are colocalized to the sensory and ganglion cells. A yeast two-hybrid assay demonstrated that the last 22 amino acids of the PPTX C terminus interact with the N terminus of Kv4.2. Chinese hamster ovary cells transfected with recombinant PPTX reveal secretory products in both non-truncated and truncated forms. Among the secreted variants are several blocked by Brefeldin A, suggesting export via a classical pathway. PPTX is soluble in the presence of sodium carbonate, suggesting localization to the cytosolic side of the plasmalemma. Immunohistochemical studies show that Kv4.2 and PPTX colocalize in the region of the plasmalemma of Chinese hamster ovary cells; however, both are locked in the endoplasmic reticulum of COS-7 cells, suggesting that PPTX does not act as a shuttle protein. Reverse transcription-PCR demonstrates that PPTX mRNA is found in tissues that include brain, eye, heart, and blood vessels.
authors.affiliation: University of South Florida, Department of Otolaryngology, Head and Neck Surgery, Tampa, Florida 33612, USA.
authors.forename: Dmytro
authors.initials: D
authors.lastname: Duzhyy
authors.forename: Margaret
authors.initials: M
authors.lastname: Harvey
authors.forename: Bernd
authors.initials: B
authors.lastname: Sokolowski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000737:chemistry*; D016466:CHO Cells; D019556:COS Cells; D002254:Carbonates / Q000494:pharmacology; D002642:Chick Embryo; D003051:Cochlea / Q000378:metabolism; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D051379:Mice; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011188:Potassium / Q000737:chemistry; D015221:Potassium Channels / Q000737:chemistry*; D024642:Potassium Channels, Voltage-Gated / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051672:Shal Potassium Channels; D015398:Signal Transduction; D014018:Tissue Distribution; D014162:Transfection; D020798:Two-Hybrid System Techniques; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D002254:Carbonates; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D010455:Peptides; D011188:Potassium; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D051672:Shal Potassium Channels; D000099153:Pentraxins; C005686:sodium carbonate