Rearrangements of the telomeric region of mouse chromosome 11 in Pre-B ABL/MYC cells revealed by mBANDing, spectral karyotyping, and fluorescence in-situ hybridization with a subtelomeric probe.
Previous work has demonstrated that chromosome 11 is trisomic or shows a duplicated region encompassing the E1/E2 band of chromosome 11 in 90% of v-abl/myc-induced plasmacytomas (Wiener et al. 1995). In the present report, we have studied BALB/c PreB lymphocytes that were immortalized by v-abl and stably transfected with a conditional MycER vector (Mai et al. 1999). These cells, termed PreB ABL/MYC, showed changes in the E1/E2 bands of chromosome 11 that are similar to those reported previously for v-abl/myc-induced plasmacytomas. This was shown by the use of chromosome painting, SKY, FISH and mBAND. Our findings suggest that the Pre-B ABL/MYC cells may be used to analyse the genetic changes affecting chromosome 11 that are associated with v-abl/myc-dependent tumorigenesis in mouse B cells.
Benedek
Katalin
K
Microbiology and Tumorbiology Centre, Karolinska Institute, Stockholm, Sweden.
Chudoba
Ilse
I
Klein
George
G
Wiener
Francis
F
Mai
Sabine
S
D000818:Animals; D001402:B-Lymphocytes; D002461:Cell Line, Transformed; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005814:Genes, Viral; D016313:Genes, abl; D016259:Genes, myc; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D014162:Transfection