Increasing the antioxidant defense in WEHI7.2 cells results in a more tumor-like metabolic profile.
Chronic inflammation is often associated with increased cancer frequency. Continuous exposure to reactive oxygen species, as at the site of chronic inflammation, can result in cells with increased antioxidant defense enzymes. In WEHI7.2 cells, overexpression of catalase or thioredoxin by transfection or selection of a cell population resistant to hydrogen peroxide has resulted in WEHI7.2 variants with altered glucose and energy metabolism. This metabolic change would favor survival in a tumor environment. We conclude that metabolic alterations, due to increased antioxidant enzyme expression, may underlie the increased tumorigenicity seen previously in the variants and contribute to the increased tumor risk associated with chronic inflammation.
Tome
Margaret E
ME
Department of Pathology, University of Arizona, Tucson, AZ 85724, USA. mtome@u.arizona.edu
Briehl
Margaret M
MM
Lutz
Norbert W
NW
D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D045744:Cell Line, Tumor; D006019:Glycolysis; D006031:Glycosylation; D006593:Hexokinase / Q000378:metabolism; D051379:Mice; D009369:Neoplasms / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism
D000596:Amino Acids; D000975:Antioxidants; D013654:Taurine; D001224:Aspartic Acid; D000255:Adenosine Triphosphate; D006593:Hexokinase