title: Analysis and determination of biological activity of short-chain peptides from porcine brain hydrolysate.
abstract: Gel permeation chromatography fractions of short-chain peptides from a hydrolysate product, which in turn was from the purified porcine brain through enzyme hydrolysis, were tested for their biological activities. The results showed that the fractions A4 and A5 had significant biological activities. The two fractions were analyzed with analytical techniques such as high-performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE), isoelectric focusing (IEF), discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Preliminary results showed that the main components of these two fractions were short-chain acidic peptides with a relative molecular mass (M(r)) of less than 2400.
authors.affiliation: Department of Analytical Chemistry, School of Pharmaceutical Science, Capital University of Medical Sciences, Beijing 100054, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Zhang
authors.forename: Bingjiu
authors.initials: B
authors.lastname: Xu
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D051379:Mice; D010455:Peptides / Q000032:analysis* / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000032:analysis* / Q000494:pharmacology; D013552:Swine
chemical_list: D010455:Peptides; D011492:Protein Hydrolysates