A method for determining intracellular concentrations of enzyme substrates from a combination of competitive inhibition and mutagenesis studies.
Copeland
Robert A
RA
Department of Enzymology and Mechanistic Pharmacology and Department of Oncology Biology, GlaxoSmithKline, 1250 S. Collegeville Road, Collegeville, PA 19426, USA. robert.a.copeland@gsk.com
Luo
Lusong
L
Auger
Kurt R
KR
Huang
Pearl S
PS
D001667:Binding, Competitive; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D042541:Intracellular Space / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D016296:Mutagenesis
D004791:Enzyme Inhibitors; D004798:Enzymes; D008024:Ligands