Analysis of leukocyte binding to depletion filters: role of passive binding, interaction with platelets, and plasma components.
Since limited knowledge exists on the mechanisms which regulate cell binding to leukocyte removal filter surfaces, we investigated the binding patterns of leukocytes to individual layers of leukocyte depletion filters. After passage of 1 unit of whole blood, blotting of isolated filter layers on glass slides or elution of cells from filter layers revealed that most leukocytes were located within the first 10 of a total of 28 filter layers, peaking at layers 6 to 8, with granulocytes binding on average to earlier filter layers than lymphocytes. Leukocytes preincubated with inhibitors of actin activation showed unchanged distribution between filter layers, suggesting that cytoskeletal activation does not significantly contribute to their binding. When leukocytes were directly incubated with single filter layers, binding of up to 30% of input cells was recorded in the absence of Ca(2+). Immunohistological analyses showed colocalization of platelets and leukocytes, with co-clustering of platelets and leukocytes. Monocytes and to some degree lymphocytes but not granulocytes competed with platelets for filter binding. Precoating of filter layers with individual plasma components showed that hyaluronic acid, plasma type fibronectin, and fibrinogen all increased the binding of leukocytes compared with albumin coating. In conclusion, leukocytes can bind passively to filters in a process which does not require Ca(2+), which is independent of cytoskeletal activation and which may depend on individual plasma components. These results are of importance when new selective cell enrichment or depletion strategies through specific filters are envisaged.
Henschler
R
R
Institute of Transfusion Medicine and Immune Hematology, Blutspendedienst Baden-Württemberg-Hessen, Sandhofstrasse 1, 60528, Frankfurt, Germany. rhenschler@blutspende.de
Rüster
B
B
Steimle
A
A
Hansmann
H L
HL
Walker
W
W
Montag
T
T
Seifried
E
E
D001773:Blood Cells / Q000166:cytology; D001792:Blood Platelets / Q000166:cytology; D001798:Blood Proteins / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion; D002450:Cell Communication; D003599:Cytoskeleton; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D047589:Leukocyte Reduction Procedures; D007962:Leukocytes / Q000166:cytology*
D001798:Blood Proteins; D002118:Calcium