title: Lack of compensatory increase in islet blood flow and islet mass in GK rats following 60% partial pancreatectomy.
abstract: The effects of a 60% partial pancreatectomy were studied in hyperglycemic GK (Goto-Kakizaki) rats. Partial pancreatectomy or a sham operation was performed on 12-week-old female Wistar rats, GK rats or hybrids between male GK rats and female Wistar rats. Measurements of pancreatic blood flow and islet blood flow were performed by a microsphere technique 2 weeks after surgery. Glucose tolerance was decreased in hybrid compared with Wistar rats, and in GK rats compared with both hybrid and Wistar rats before surgery. Partial pancreatectomy induced minor changes in glucose tolerance. Wistar rats had a decreased islet mass following partial pancreatectomy. Both hybrid and GK rats showed a significant decrease in relative islet volume, but only GK rats in total islet mass, compared with Wistar rats 2 weeks after surgery. Pancreatic blood flow and islet blood flow did not significantly differ between sham-operated Wistar, hybrid or GK rats. After partial pancreatectomy, islet blood flow in relation to islet mass increased 3-fold in Wistar rats and 2-fold in hybrid rats. In contrast, GK rats showed no increase in islet blood flow following partial pancreatectomy. It is concluded that compensatory mechanisms after partial pancreatectomy are operating less efficiently in hybrid and GK rats.
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
authors.forename: Annika M
authors.initials: AM
authors.lastname: Svensson
authors.forename: Claes-Göran
authors.initials: CG
authors.lastname: Ostenson
authors.forename: Birgitta
authors.initials: B
authors.lastname: Bodin
authors.forename: Leif
authors.initials: L
authors.lastname: Jansson
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000503:physiopathology / Q000601:surgery*; D005260:Female; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000098:blood supply* / Q000473:pathology*; D008297:Male; D023421:Models, Animal; D010179:Pancreas / Q000098:blood supply; D010180:Pancreatectomy; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012039:Regional Blood Flow
chemical_list: D007328:Insulin