[Functional analysis of vernalization-related gene VER17 in flower development using antisense RNA strategy in winter wheat].
To understand the function of vernalization-related gene VER17 in winter wheat (Triticum aestivum L. cv. Jingdong No.1), an antisense RNA strategy was used. The antisense VER17 with a vector pBI121 was constructed and transformed into winter wheat by using the pollen-tube-pathway method. Fourteen independent transgenic plants transformed with antisense VER17 and five control transformants transformed with pBI121 blank vector were obtained and confirmed by GUS histochemical assay and PCR-Southern blot analysis. Phenotypes of T(0) and T(1) transgenic plants showed that the plants of the antisense VER17 transgenic lines degenerated top or basal spikelets and had delayed flowering time, which suggested that the VER17 gene functions in accelerating flowering and the development of the top or basal spikelets and the stamen which were impacted by vernalization treatment.
Xu
Wen-Zhong
WZ
Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Yong
Wei-Dong
WD
Xu
Yun-Yuan
YY
Liang
Tie-Bing
TB
Bai
Wei
W
Chong
Kang
K
Tan
Ke-Hui
KH
Xu
Zhi-Hong
ZH
Zhu
Zhi-Qing
ZQ
D015139:Blotting, Southern; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016372:RNA, Antisense / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012621:Seasons; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D010940:Plant Proteins; D016372:RNA, Antisense; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase