[Analysis of protein expression maps of two metastatic colorectal carcinoma cell lines generated by two-dimensional gel electrophoresis].
OBJECTIVE
To analyze the differentially expressed proteins in metastatic colorectal carcinoma and provide clues for the molecular mechanisms of colorectal cancer metastasis.


METHODS
A pair of colorectal carcinoma cell lines SW620 and SW480 with different metastatic potentials from the same parent cell lines were studied. The protein expression maps of the two cell lines were obtained and optimized using two-dimensional gel electrophoresis (2-DE), and the differentially expressed protein spots associated with metastasis were analyzed by Melanie III software.


RESULTS
The 2-DE expression maps of the two cell lines were well matched. The resolution of 2-DE was increased after using the nonlinear pH3-10 or overlapping narrow immobilized pH gradient strips, and 11 differentially expressed protein spots were identified after analysis using the software.


CONCLUSIONS
Nonlinear pH3-10 and overlapping narrow immobilized pH gradient strips have higher separation efficiency for protein in 2-DE. The differentially expressed proteins separated will be useful for identifying metastasis-associated proteins of colorectal carcinoma and for establishing 2-DE database of the metastatic colorectal carcinoma cell lines.
Liang
Li
L
Department of Pathology, Southern Medical University, Guangzhou 510515, China. lli@fimmu.com
Ding
Yan-qing
YQ
Li
Xin
X
Jiang
Pei-zhou
PZ
Li
Xue-hua
XH
Zhang
Jin-hua
JH
D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis*; D010449:Peptide Mapping
D009363:Neoplasm Proteins