A continuous-flow perfusion system for the maintenance and NMR study of small tissue samples in vitro.
To describe and evaluate a novel perfusion system developed to maintain excised tissue in a flowing, oxygenated bathing solution during acquisition of nuclear magnetic resonance (NMR) data, and in addition allow precise data to be acquired continuously while altering the composition of the bathing solution surrounding the tissue. A chamber to house the tissue sample was constructed of interlocking sections of polyethylene tubing, and had approximate internal dimensions of 4 mm in diameter and 4 mm in height. Temperature-controlled, physiologically appropriate buffer solution was pumped via an infusion pump through the chamber, entering and exiting by way of small openings on either end. Immediately surrounding the polyethylene chamber was a tight-fitting four-loop solenoid RF coil. Measured proton NMR parameters were found to be fairly insensitive to the flow rate of the buffer if this coil was used only for reception and a larger-volume transmit-only coil was used for excitation. Temperature control of the sample was successfully implemented between 25 and 40 degrees C. The perfusion system was found to be resistant to the effects of flow rate, as well as a useful tool for the administration of drugs or agents to the tissue. Changes in buffer composition could be performed on the fly without the need to reposition the sample each time a change was made. This avoidance of repositioning was found to yield a fivefold improvement in the precision of T(2) spectral parameters (using frog sciatic nerve as a sample).
Wachowicz
K
K
Department of Biomedical Engineering, 1098 Research Transition Facility, University of Alberta, Edmonton, Canada. wachow@ieee.org
Snyder
R E
RE
D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods; D008280:Magnetics / Q000295:instrumentation*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods; D011898:Ranidae; D012584:Sciatic Nerve / Q000737:chemistry / Q000502:physiology; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods; D014159:Transducers