title: [Studies on petal tissue culture of the traditional Chinese medicine Chrysanthemum morifolium].
abstract: OBJECTIVE
  To set up the optimums for the petal tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.
  
  
  METHODS
  Petals were cut and cultured onto the same kind of media on different sides and different kinds of media on the same side, and induced to form the whole plantlet.
  
  
  CONCLUSIONS
  All the media used could induce callus, while the results of callus regeneration was distinct. The MS medium with KT 2 mg x L(-1) and NAA 0.2 mg x L(-1) was much better than others when callus were induced. The MS medium with KT 2 mg x L(-1) + NAA 0.2 mg x L(-1) and AgNO3 5 mg x L(-1) was the preferable medium for the bud sprouting. The frequency of plantlets regeneration which was induced by flower bud was higher than that by opening petal. The regenerated plantlets shod produced variation of leaf and plant shape.
authors.affiliation: Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, China. xuejp2000@yahoo.com.cn
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Chang
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
mesh_terms: D031202:Chrysanthemum / Q000254:growth & development*; D003470:Culture Media; D035264:Flowers / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000254:growth & development*; D012835:Silver Nitrate / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D012835:Silver Nitrate