[Inhibitory effect of p21WAF1 on squamous cell carcinoma of larynx mediated with EGF-R targeting polypeptide gene delivery system].
OBJECTIVE
To evaluate the efficiency of EGF-R targeted polypeptide gene delivery system and inhibitory effect of p21WAF1 on squamous cell carcinoma of larynx.


METHODS
The EGF-R targeting polypeptide gene delivery system was constructed and a therapeutic gene of p21WAF1 were transfected into Hep-2 cells with the new gene delivery system. The growth curve of transfected Hep-2 cells and flow cytometric analysis were applied to assess the transferring and expression of exogenous genes and inhibitory effect on Hep-2 cells.


RESULTS
With the transferring of p21WAF1 gene, the growth of Hep-2 cells was inhibited significantly by 79%, and the apoptosis was observed by means of flow cytometric analysis.


CONCLUSIONS
EGF-R targeting polypeptide gene delivery system could transfer the therapeutic gene into targeted EGF-R expressing Hep-2 cells and the expression of p21WAF1 could inhibit the growth of Hep-2 cells and induced the cells to apoptosis.
Xiao
Shuifang
S
Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Beijing University, Beijing, 100034, China.
Wang
Chengyuan
C
Li
Tiancheng
T
Tian
Peikun
P
Gu
Jianren
J
D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D007822:Laryngeal Neoplasms / Q000628:therapy*; D014162:Transfection; D014407:Tumor Cells, Cultured
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D066246:ErbB Receptors