title: Type 2 heat-labile enterotoxin (LT-II)-producing Escherichia coli isolated from ostriches with diarrhea.
abstract: The culture supernatant of Escherichia coli, isolated from ostriches with diarrhea in Brazil, caused elongation in Vero cell, rounding in Chinese hamster ovary (CHO) cells and a cytoplasmic vacuolation in ostrich embryo fibroblasts (OEF), but it was not cytotoxic for chicken embryo fibroblasts (CEF). These effects were not neutralized by antiserum to cholera toxin. Polymerase chain reaction assays showed that the ostrich E. coli contained the gene encoding (eltII-A), but not those for type 1 heat-labile enterotoxin (eltA), heat-stable enterotoxins (estA, estB), verocytotoxins (stx-I, stx-II), or cytotoxic necrotizing factors (cnf 1, cnf 2). All isolates belonged to serotype O15:H8. The enteropathogenic relevance of LT-II in ostrich diarrhea remains undetermined.
authors.affiliation: Faculdade de Medicina Veterinária, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Jaboticabal, SP, Brazil.
authors.forename: Ana Rita M
authors.initials: AR
authors.lastname: Nardi
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Salvadori
authors.forename: Lia T
authors.initials: LT
authors.lastname: Coswig
authors.forename: Maria Sílvia V
authors.initials: MS
authors.lastname: Gatti
authors.forename: Domingos S
authors.initials: DS
authors.lastname: Leite
authors.forename: Geórgio F
authors.initials: GF
authors.lastname: Valadares
authors.forename: Manoel Garcia
authors.initials: MG
authors.lastname: Neto
authors.forename: Rubén P
authors.initials: RP
authors.lastname: Shocken-Iturrino
authors.forename: Jesús E
authors.initials: JE
authors.lastname: Blanco
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Yano
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001715:Bird Diseases / Q000382:microbiology*; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis*; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D020310:Struthioniformes / Q000382:microbiology*; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli