Tumor necrosis factor receptor 1 and 2 proteins are differentially regulated during Wallerian degeneration of mouse sciatic nerve.
The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF) is involved in injury-induced peripheral nerve pathology and in the generation of neuropathic pain. Here, we investigated local protein levels of the two known TNF receptors, TNF receptor 1 and 2 (TNFR1, TNFR2), on days 0, 1, 3, 7, 14, and 28 after unilateral crush or chronic constriction injury (CCI) of mouse sciatic nerves using enzyme-linked immunoassay. Both receptors were detectable at a low level in nerve homogenates from naive mice. After crush or CCI, TNFR1 increased by 2-fold on days 3 and day 7. Unlike TNFR1, TNFR2 was markedly upregulated already on day 1 after crush or CCI. TNFR2 increased by 7-fold on days 3 and 7, and remained elevated at a lower level until day 28 after both CCI and crush injury. These data indicate that endoneurial TNFR1 and TNFR2 proteins are differentially regulated during Wallerian degeneration.
George
Annette
A
Neurologische Klinik der Universität, Josef-Schneider-Str. 11, 97080 Würzburg, Germany.
Buehl
Achim
A
Sommer
Claudia
C
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008026:Ligation; D051379:Mice; D008810:Mice, Inbred C57BL; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology; D010525:Peripheral Nerves / Q000378:metabolism; D010523:Peripheral Nervous System Diseases / Q000378:metabolism* / Q000503:physiopathology; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism*; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D020426:Sciatic Neuropathy / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology; D014855:Wallerian Degeneration / Q000378:metabolism* / Q000503:physiopathology
D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha