title: Dendrimer interactions with hydrophobic fluorescent probes and human serum albumin.
abstract: The interactions between three types of polyamidoamine dendrimers (with anionic, cationic, and neutral charge on a surface) and fluorescent dye 1-anilinonaphthalene-8-sulfonate (ANS) were studied. Double fluorimetric titration method was employed to estimate a binding constant and the number of binding centers. As fluorescent probes can serve as models of toxin molecules, dendrimers, and human serum albumin (HSA) abilities to bind ANS were compared. In the presence of HSA and dendrimers, ANS located both in HSA and in dendrimers, but the interactions between ANS and HSA were stronger.
authors.affiliation: Institute of Photobiology of Natl. Ac. Sci. Bel., Akademicheskaya 27, Minsk 220072, Belarus.
authors.forename: D
authors.initials: D
authors.lastname: Shcharbin
authors.forename: B
authors.initials: B
authors.lastname: Klajnert
authors.forename: V
authors.initials: V
authors.lastname: Mazhul
authors.forename: M
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D001665:Binding Sites; D050091:Dendrimers; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D011073:Polyamines / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000817:Anilino Naphthalenesulfonates; D050091:Dendrimers; D005456:Fluorescent Dyes; C104700:PAMAM Starburst; D011073:Polyamines; D012709:Serum Albumin; C027132:1-anilino-8-naphthalenesulfonate