title: Positron-emission tomography imaging of early events after transplantation of islets of Langerhans.
abstract: The aim of our study was to assess cell trafficking and early events after intraportal islet transplantation. Sprague-Dawley rat islets were incubated for various times, with various concentrations of 2-[F]fluoro-2deoxy-D-glucose (FDG), and in presence of various glucose concentrations. FDG-labeled syngeneic islets or FDG alone were injected in rats. Radioactivity was measured in the liver and in various organs by positron-emission tomography for 6 hours. FDG uptake increased with incubation time or FDG concentration and decreased in presence of glucose. In vivo, all islets implanted in the liver, with an uptake 4.4 times higher than controls (44.2% vs. 10.1%, P=0.02). Radioactivity in the liver decreased at the same rate after injection of labeled-islets and FDG alone. Ex vivo labeling of islets and imaging of posttransplant early events were feasible. Islets engrafted exclusively in the liver. No islet loss could be demonstrated 6 hours after transplantation.
authors.affiliation: Cell Isolation and Transplantation Center, Department of Surgery, Geneva University Hospitals, Geneva, Switzerland. christian.toso@hcuge.ch
authors.forename: Christian
authors.initials: C
authors.lastname: Toso
authors.forename: Habib
authors.initials: H
authors.lastname: Zaidi
authors.forename: Philippe
authors.initials: P
authors.lastname: Morel
authors.forename: Mathieu
authors.initials: M
authors.lastname: Armanet
authors.forename: Axel
authors.initials: A
authors.lastname: Andres
authors.forename: Nadine
authors.initials: N
authors.lastname: Pernin
authors.forename: Reto
authors.initials: R
authors.lastname: Baertschiger
authors.forename: Daniel
authors.initials: D
authors.lastname: Slosman
authors.forename: Leo H
authors.initials: LH
authors.lastname: Bühler
authors.forename: Domenico
authors.initials: D
authors.lastname: Bosco
authors.forename: Thierry
authors.initials: T
authors.lastname: Berney
mesh_terms: D000818:Animals; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics; D007515:Islets of Langerhans / Q000000981:diagnostic imaging*; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D008297:Male; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18