Immunochemical analysis of lipopolysaccharide-like component extracted from Borrelia burgdorferi sensu lato.
Immunoelectrophoresis and its modifications were applied to analysis of a lipopolysaccharide-like component (LPS-LC) extracted from Borrelia garinii strains K24 and K48 isolated from Ixodes ricinus and Borrelia burgdorferi sensu stricto strain B31. A modification of the hot phenol-water method was used for isolation of LPS. Immunoelectrophoresis (IE) and crossed immunoelectrophoresis (CIE) of LPS-LC with polyclonal rabbit antisera revealed a pattern and properties partially similar to LPS from other Gram-negative bacteria. B. garinii LPS-LC formed in CIE a diffuse band extending from the start to the anode. Similarly, the shape and position of the band in IE did not show major differences from LPS of other Gram-negative bacteria. The LPS-LC extracted from the three genomic groups of B. burgdorferi sensu lato were found to have similar immunochemical properties irrespective of their genotype origin.
Schwarzová
K
K
Institute of Preventive and Clinical Medicine, Slovak Health University, Bratislava, Slovakia. virukats@savba.sk
Ciznár
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D000818:Animals; D000907:Antibodies, Bacterial; D001483:Base Sequence; D001898:Borrelia / Q000737:chemistry / Q000276:immunology; D025065:Borrelia burgdorferi / Q000737:chemistry / Q000276:immunology; D015748:Borrelia burgdorferi Group / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D004269:DNA, Bacterial / Q000235:genetics; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D008070:Lipopolysaccharides