title: [Influence of sargassum fusiforme polysaccharide on apoptosis of tumor cells].
abstract: OBJECTIVE
  To study mechanism of antitumor activity of Sargassum Fusiforme Polysaccharide (SFPS).
  
  
  METHODS
  The effect on cell cycle and apoptosis was studied with flow cytometry (FCM). Intracellular calcium concentration [Ca2+]i was marked with Fluo-3/AM and measured with laser scanner confocal microscope (LSCM).
  
  
  RESULTS
  SFPS inhibited G0/G1 stage SGC-7901 from entering to S stage and increased APO%. The [Ca2+]i showed a transient rise and return to the original level. The concentration could be raised again by administering CaCl2.
  
  
  CONCLUSIONS
  The antitumor effect of SFPS seems to be accomplished through the apoptosis associated with the increase in intracellular calcium concentration. Intracellular stores release the calcium during its action.
authors.affiliation: Postdoctoral Programme, the Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China. jyb@hrbcu.edu.cn
authors.forename: Yu-bin
authors.initials: YB
authors.lastname: Ji
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Gao
authors.forename: Xiu-juan
authors.initials: XJ
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D002122:Calcium Chloride / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D006801:Humans; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D002122:Calcium Chloride; D002118:Calcium