Adverse effects of bisphenol A to spermiogenesis in mice and rats.
Either a 20 or 200 microg/kg body weight/injection of bisphenol A (BPA) was subcutaneously administered to adult mice and rats for 6 days, and the effects on the testes were investigated by electron and light microscopy. Abnormalities were observed in the spermatids: acrosomal vesicles, acrosomal caps, acrosomes and nuclei of the spermatids were severely deformed. The ectoplasmic specialization between the Sertoli cell and spermatids were also affected: incomplete specialization, redundant ectopic specialization and aplasia were observed. Rats and mice responded similarly to BPA. There were no dose dependencies between the 20- and 200 microg/kg body weight/injection groups. The ectoplasmic specialization between adjoining Sertoli cells, or blood-testis barrier, was not affected. Since similar adverse effects were observed when adult mice were treated with beta-estradiol 3-benzoate, the effects of BPA reported here seem to reflect the estrogenic effects on the testes. Animals kept for an additional two months after cessation of the administration were shown to be fertile and the testes showed normal histology, indicating that the adverse effects were transitory.
Toyama
Yoshiro
Y
Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University, Chiba, Japan.
Suzuki-Toyota
Fumie
F
Maekawa
Mamiko
M
Ito
Chizuru
C
Toshimori
Kiyotaka
K
D000177:Acrosome / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D001559:Benzhydryl Compounds; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000187:drug effects / Q000648:ultrastructure; D013087:Spermatids / Q000187:drug effects / Q000648:ultrastructure; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000648:ultrastructure; D013997:Time Factors; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds