Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes.
We developed a duplex PCR assay targeting the hemagglutinin multigene families, vlhA and pMGA, of Mycoplasma synoviae and Mycoplasma gallisepticum, respectively. The assay proved to be specific and sensitive enough to justify its use for the simultaneous detection of the two major avian mycoplasma species from field isolates.
Mardassi
B Ben Abdelmoumen
BB
Laboratoire des Mycoplasmes. Institut Pasteur de Tunis, Tunis, Tunisia. boutheina.mardassi@pasteur.rns.tn
Mohamed
R Ben
RB
Gueriri
I
I
Boughattas
S
S
Mlik
B
B
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D017124:Conserved Sequence; D006388:Hemagglutinins / Q000235:genetics*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary; D045686:Mycoplasma gallisepticum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D045805:Mycoplasma synoviae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases / Q000382:microbiology; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014132:Trachea / Q000382:microbiology
D001426:Bacterial Proteins; D006388:Hemagglutinins