The expression of apoptosis related genes in the first trimester human placenta using a short term in vitro model.
Using in vitro model for studying the induction and inhibition of spontaneous apoptosis in human first trimester placental villi, mediated by the free radical scavenger SOD, we have examined the expression of bcl-xL, bax, Caspase-3 and PARP (Poly ADP-ribosyl). An increase in apoptosis was associated with activation of PARP and an increase and activation of Caspase-3. There was no significant change in bcl-x or bax. Therefore bcl-x and bax do not appear to have a significant role in apoptosis in the first trimester in vitro. Cleavage of Caspase-3 rather than transcriptional regulation appears to be the main determinant of Caspase-3 activity in first trimester placental villi.
Charles
A K
AK
Department of Pathology King Edward Memorial Hospital, University of Western Australia, Perth, Western Australia. adrian.charles@health.wa.gov.aau
Hisheh
S
S
Liu
D
D
Rao
R M
RM
Waddell
B J
BJ
Dickinson
J E
JE
Rao
A J
AJ
Dharmarajan
A M
AM
D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D018929:Cell Culture Techniques; D002824:Chorionic Villi / Q000502:physiology*; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D006801:Humans; D010920:Placenta; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012333:RNA, Messenger / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism
D016166:Free Radical Scavengers; D012333:RNA, Messenger; D013482:Superoxide Dismutase; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases