The large scale generation of dendritic cells for the immunization of patients with non-small cell lung cancer (NSCLC).
In the current study, we generated large numbers of dendritic cells (DCs) from patients with non-small cell lung cancer (NSCLC) for a vaccine trial. The DCs were generated from CD14+ cells obtained by immuno-magnetic bead column separation technique. The CD14+ cells were placed in culture in the presence of granulocyte macrophage colony stimulating factor (GMCSF) and Interleukin 4 (IL-4). At Day 7, apoptotic bodies derived from an allogeneic NSCLC line 1650-TC were added to the cultures at a DC:tumor cell ratio of 1:1. At Day 8, the DCs were harvested, washed and injected intradermally into patients. Using this protocol we have prepared DCs for 16 patients. An average of 9.3 x 10(7) DCs was injected for the priming dose and 8.2 x 10(7) DCs for the boost. Clinical evaluation of the patients and immune assessment are presented in a separate report. The current report provides evidence for the large scale production of functional DCs derived from patients with NSCLC which can be used as vaccines in clinical trials.
Yannelli
John R
JR
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky School of Medicine, Markey Cancer Center, 800 Rose Street, Lexington, Kentucky 40536, USA. Jyann1@email.uky.edu
Sturgill
Jamie
J
Foody
Terry
T
Hirschowitz
Edward
E
D017209:Apoptosis; D019496:Cancer Vaccines; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology* / Q000517:prevention & control*; D018929:Cell Culture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006650:Histocompatibility Testing; D006801:Humans; D018189:Immunomagnetic Separation; D015847:Interleukin-4 / Q000494:pharmacology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology* / Q000517:prevention & control*; D010641:Phenotype
D019496:Cancer Vaccines; D018950:Lipopolysaccharide Receptors; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor