Biotin-phenyldiazomethane conjugates as labeling reagents at phosphate in mono and polynucleotides.
Molecules 2-5 that include in their structure a biotin moiety as detectable unit and differently substituted phenyl diazo functions as reactive group were prepared as reagents for labeling the phosphate group in mono and polynucleotides. These molecules were shown to react selectively and quantitatively with the model nucleotide 3'-UMP. They were used successfully in the labeling step of DNA and RNA analysis using high-density DNA-chips (or microarrays) technology.
Bourget
Cécile
C
LEDSS-UMR5616 associé au CNRS, BP53, Université Joseph Fourier, 38041 Grenoble, France.
Trévisiol
Emmanuelle
E
Bridon
Isabelle
I
Kotera
Mitsuharu
M
Lhomme
Jean
J
Laayoun
Ali
A
D001710:Biotin / Q000737:chemistry*; D003978:Diazomethane / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D009711:Nucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*
D009711:Nucleotides; D010710:Phosphates; D003978:Diazomethane; D001710:Biotin