Chemical and genomic combined approach applied to the characterization and identification of Italian Allium sativum L.
Chemotype analyses and random amplified polymorphic DNA (RAPD) genomic analyses have been applied to the characterization of Allium sativum variety from Voghiera (Ferrara, Italy), a typical Italian product actually demanding the Protected Designation of Origin (PDO). The garlic from Voghiera is characterized by peculiar morphological and composition characteristics. The proximate composition and atomic absorbance spectrometry elemental pattern of this garlic suggested as the chemical composition did not depend on the intrinsic pedologic soil features only, but it was probably connected to some peculiar genetic characters. Amplification of genomic DNA using random primers highlighted a good clustering differentiating of Voghiera Allium sativum from five commercial reference samples used in this study (Piacentino, Serena, France, China, and Adriano varieties), confirming the existence of intervarietal genetic difference. The intravarietal polymorphisms of Voghiera samples were low.
Brandolini
Vincenzo
V
Department of Pharmaceutical Science, University of Ferrara, Via Fossato di Mortara 17/19, 44100 Ferrara, Italy.
Tedeschi
Paola
P
Cereti
Elisabetta
E
Maietti
Annalisa
A
Barile
Daniela
D
Coïsson
Jean Daniel
JD
Mazzotta
Dionisio
D
Arlorio
Marco
M
Martelli
Aldo
A
D018744:DNA, Plant / Q000032:analysis; D005737:Garlic / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D007558:Italy; D008903:Minerals / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant; D008903:Minerals