[Protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline in vitro].
OBJECTIVE
To investigate the cardio-protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline.


METHODS
After adding crocetin, the primary culture of cardiac myocyte was injured by 1.0 micromol x L(-1) noradrenaline. The activity of lactic dehydrogenase (LDH), mitochondrion succinic dehydrogenase (MSDH) and ATPase were assayed. The mitochondrion membrane potential was detected by Rh123. The percentage of cardiac myocyte apoptosis was observed by flow cytometry.


RESULTS
Crocetin significantly decreased the activity of LDH in culture supernatant, increased the activity of MSDH, ATPase (Na+-K+ ATPase, Ca2+ ATPase) and mitochondrion membrane potential.


CONCLUSIONS
Crocetin could alleviate the disturbance of energy metabolism and decrease the percentage of apoptosis of cardiac myocyte treated with noradrenaline.
Shen
Xiang-chun
XC
Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China. sxc9860@sina.com
Qian
Zhi-yu
ZY
Chen
Qi
Q
Wang
Ya-juan
YJ
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002338:Carotenoids / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D027622:Crocus / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000201:enzymology*; D009638:Norepinephrine / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D014801:Vitamin A / Q000031:analogs & derivatives
D020011:Protective Agents; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D009638:Norepinephrine