Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes.
In this paper we report on the metabolic response of human hepatocytes grown on polyethersulfone membranes surface modified with a plasma-deposited acrylic acid coating and RGD peptide covalently immobilized through a "spacer arm" molecule. The modified surfaces were characterized by means of X-ray photoelectron spectroscopy and water contact angle measurements. The performance of modified and unmodified membranes was evaluated by assessing the expression of liver specific and biotransformation functions of human hepatocytes. Diclofenac, a non-steroidal anti-inflammatory drug, was used to investigate the biotransformation functions. Surface-modified membranes elicit specific cellular responses and induce hepatocytes to enhance the synthesis rate of albumin and urea, particularly in the presence of diclofenac. Also the biotransformation functions were expressed at high levels.
De Bartolo
Loredana
L
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci cubo 17/C, 87030 Rende (CS), Italy. l.debartolo@itm.cnr.it
Morelli
Sabrina
S
Lopez
Linda C
LC
Giorno
Lidietta
L
Campana
Carla
C
Salerno
Simona
S
Rende
Maria
M
Favia
Pietro
P
Detomaso
Loredana
L
Gristina
Roberto
R
d'Agostino
Riccardo
R
Drioli
Enrico
E
D000327:Adsorption; D000418:Albumins / Q000096:biosynthesis; D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D019164:Liver, Artificial; D008422:Materials Testing; D008567:Membranes, Artificial; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014508:Urea / Q000378:metabolism
D000418:Albumins; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D014508:Urea