title: [Study on tissue culture of pasqueflower].
abstract: OBJECTIVE
  To speed up seedling production of pasqueflower (Puzlsatilla chinenses) and their modernization in pasqueflower.
  
  
  METHODS
  With tissue culture method, primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment combinations of adding different hormones.
  
  
  RESULTS
  The appropriate medium for different culture stages were MS + 6-BA 1.0-3.0 mg x L(-1) + NAA 0-0.05 mg x L(-1) + Sucrose 30 g x L(-1) in primary culture, MS + 6-BA 0.2 mg x L(-1) + NAA 0.02 mg x L(-1) + BR 0.00001 mg x L(-1) + Sucrose 30 g x L(-1) in differentiation and subculture of cluster buds, 1/2 MS + NAA 0.4 mg x L(-1) + Sucrose 20 g x L(-1) in rooting.
  
  
  CONCLUSIONS
  Applying stem tip and flower buds as explants, high frequency propagation of seedlings can be achieved with plant tissue culture in Pasqueflower.
authors.affiliation: Anhui Technical Teacher College, Fengyang 233100 Anhui, China. zzxue2003@yahoo.com.cn
authors.forename: Zi-xue
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Wei-qun
authors.initials: WQ
authors.lastname: Ding
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: Wei-jing
authors.initials: WJ
authors.lastname: Shi
authors.forename: Wen-cai
authors.initials: WC
authors.lastname: Ye
mesh_terms: D035264:Flowers / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development; D031946:Pulsatilla / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D010937:Plant Growth Regulators