[Direct enantiomeric separation of pantoprazole sodium by high performance liquid chromatography].
By using the Kromasil CHI-TBB chiral stationary phase, the racemic mixture of pantoprazole sodium was resolved in the normal phase mode. The influences of the composition of the mobile phase, the content of acidic and basic additives and the flow rate on this enantiomer separation were studied. The chiral high performance liquid chromatographic method for the analysis of pantoprazole sodium was established with high efficiency and good repeatability, by using hexane-isopropanol-acetic acid mixture (95:5:0.1, v/v) as the mobile phase at a flow rate of 2.0 mL/min at 25 degrees C.
Ding
Guosheng
G
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China.
Cong
Runzi
R
Wang
Junde
J
D019840:2-Propanol / Q000737:chemistry; D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000737:chemistry / Q000302:isolation & purification*; D019342:Acetic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006586:Hexanes / Q000737:chemistry; D000077402:Pantoprazole; D054328:Proton Pump Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013696:Temperature
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D006586:Hexanes; D054328:Proton Pump Inhibitors; D000077402:Pantoprazole; D019840:2-Propanol; D019342:Acetic Acid