title: Protease susceptibility of the Caulobacter crescentus flagellar hook-basal body: a possible mechanism of flagellar ejection during cell differentiation.
abstract: When motile swarmer cells of Caulobacter crescentus differentiate into sessile stalked cells, the flagellum is ejected. To elucidate the molecular mechanism of the flagellar ejection, flagellar hook-basal body (HBB) complexes from C. crescentus were purified and characterized. The purified HBBs were less stable against acidic pH or protease treatment than HBBs of Salmonella typhimurium, supporting the view that flagellar ejection from C. crescentus is initiated by destruction of the fragile basal structures. In addition, protease treatment of the purified flagella resulted in the specific digestion of the MS ring complex, revealing for the first time the intact structure of the whole rod.
authors.affiliation: Graduate School of Natural Sciences, Nagoya City University, 1 Yamanohata, Mizuho, Nagoya 467-8501, Japan.|CREST, Japan Science and Technology Agency (JST), 1064-18 Takahori, Hirata, Takanezawa, Shioya-gun, Tochigi 329-1206, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kanbe
authors.affiliation: CREST, Japan Science and Technology Agency (JST), 1064-18 Takahori, Hirata, Takanezawa, Shioya-gun, Tochigi 329-1206, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.affiliation: CREST, Japan Science and Technology Agency (JST), 1064-18 Takahori, Hirata, Takanezawa, Shioya-gun, Tochigi 329-1206, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Umino
authors.affiliation: Division of Molecular Microbiology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland.
authors.forename: U
authors.initials: U
authors.lastname: Jenal
authors.affiliation: CREST, Japan Science and Technology Agency (JST), 1064-18 Takahori, Hirata, Takanezawa, Shioya-gun, Tochigi 329-1206, Japan.
authors.forename: S-I
authors.initials: SI
authors.lastname: Aizawa
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016935:Caulobacter crescentus / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D005407:Flagella / Q000502:physiology* / Q000648:ultrastructure*; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009024:Morphogenesis; D010447:Peptide Hydrolases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010447:Peptide Hydrolases