Simultaneous determination of trace of loxacin, ciprofloxacin, and sparfloxacin by micelle TLC-fluorimetry.
The method of simultaneous determination of ofloxacin (OFLX), ciproflxacin (CPLX), and sparfloxacin (SPLX) by thin-layer chromatography is established, with micelle solutions as mobile phases. It is found that the optimum molar ratio of sodium dodecyl sulfate (SDS) to ethylene diamine tetraacetic acid is 0.01:0.1. On the polyamide thin-layer sheet, OFLX, CPLX, and SPLX are separated from each other, and the corresponding Rf values are 0.72, 0.55, and 0.32, respectively. The fluorescence spots are scanned with a spectrodensitometer at the excitation wavelength of 282 nm. The cut-off filter is set at 400 nm. The detection limits are 2 x 10(-6) mol/L for OFLX, 1.5 x 10(-6) mol/L for CPLX, and 1.6 x 10(-6) mol/L for SPLX, and the respective linear ranges correspondingly fell in the concentration of 1 x 10(-5) to 4 x 10(-4) mol/L for OFLX, 1 x 10(-5) to 4.5 x 10(-4) mol/L for CPLX, and 1 x 10(-5) to 4.2 x 10(-4) mol/L for SPLX. For all the three components, the relative standard deviations are in the range of 1.12-5.82%, and the recoveries are found to be 96.7-104.2% in urine and serum samples.
Feng
Yu-Lin
YL
The Institute of Modern Chemistry, Shanxi University, Taiyuan 030006, PR China. ylfeng@sxu.edu.cn
Dong
Chuan
C
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis*; D005470:Fluorometry / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis*; D008823:Micelles; D015242:Ofloxacin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D008823:Micelles; D002939:Ciprofloxacin; D015242:Ofloxacin; C061363:sparfloxacin