Influence of N,N'-diacetyl-L-cystine on D-galactosamine/lipopolysaccharide induced immunological liver failure in mice.
OBJECTIVE
To study the therapeutic effects of N,N'-diacetyl-L-cystine (DiNAC) on immunological liver failure.


METHODS
Serum ALT, AST and T cell subsets in peripheral blood of the experimental animals during the trial period were analyzed by an automatic serum analyzer and a flow cytometer, respectively. The sectioned liver specimens were examined under a light microscope. And 24 h after the injection of Gal/LPS, the survival rate of rats was calculated.


RESULTS
DiNAC (50, 200, 800 mg x kg(-1), i.p.) suppressed the elevation of serum levels of ALT and AST, markedly enhanced proliferation and differentiation of T cell subsets (CD4+, CD8+ and Th1, Th2), and improved all the histopathological features. In mice of fulminant hepatic failure (FHF), the survival time significantly prolonged and the survival rate increased 24 h after i.p. DiNAC. These effects were obviously dose-dependent.


CONCLUSIONS
DiNAC on mice with FHF has an inhibitory action which is related to immune mechanism.
Wang
Pei
P
Department of Clinical Pharmacology, The Second Affiliated Hospital, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310007, China. wangp_0209@hotmail.com
Ren
Xing-chang
XC
Yu
Jin
J
Lin
Yi
Y
Wu
Xi-ming
XM
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003553:Cystine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008099:Liver / Q000473:pathology*; D017114:Liver Failure, Acute / Q000097:blood / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000473:pathology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D003553:Cystine; C056709:N,N-diacetylcystine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin