title: Database independent detection of isotopically labeled MS/MS spectrum peptide pairs.
abstract: Mass spectrometry data generated in differential profiling of complex protein samples are classically exploited using database searches. In addition, quantitative profiling is performed by various methods, one of them using isotopically coded affinity tags, where one typically uses a light and a heavy tag. Here, we present a new algorithm, ICATcher, which detects pairs of light/heavy peptide MS/MS spectra independent of sequence databases. The method can be used for de novo sequencing and detection of posttranslational modifications. ICATcher is distributed as open source software.
authors.affiliation: Functional Genomics Center Zurich, Uni/ETH Zurich, Winterthurer Strasse 190, 8057 Zurich, Switzerland. frank@fgcz.ethz.ch
authors.forename: Frank
authors.initials: F
authors.lastname: Potthast
authors.forename: Jiri
authors.initials: J
authors.lastname: Ocenasek
authors.forename: Dorothea
authors.initials: D
authors.lastname: Rutishauser
authors.forename: Martin
authors.initials: M
authors.lastname: Pelikan
authors.forename: Ralph
authors.initials: R
authors.lastname: Schlapbach
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*
chemical_list: D010455:Peptides