Simultaneous and selective production of levan and poly(gamma-glutamic acid) by Bacillus subtilis.
Bacillus subtilis(natto) Takahashi, used to prepare the fermented soybean product natto, was grown in a basal medium containing 5% (w/w) sucrose and 1.5% (w/w) L-glutamate and produced 58% (w/w) poly(gamma-glutamic acid) and 42% (w/w) levan simultaneously. After 21 h, 40-50 mg levan ml-1 had been produced in medium containing 20% (w/w) sucrose but without L-glutamate. In medium containing L-glutamic acid but without sucrose, mainly poly(gamma-glutamic acid) was produced.
Shih
Ing-Lung
IL
Department of Environmental Engineering, Da-Yeh University, Chang-Hwa, Taiwan. ils@mail.dyu.edu.tw
Yu
Yun-Ti
YT
D001412:Bacillus subtilis / Q000378:metabolism*; D003470:Culture Media; D005630:Fructans / Q000096:biosynthesis* / Q000737:chemistry; D018698:Glutamic Acid / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D011099:Polyglutamic Acid / Q000096:biosynthesis* / Q000737:chemistry; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D005630:Fructans; D011099:Polyglutamic Acid; D018698:Glutamic Acid; D013395:Sucrose; C072599:levan