Identification of genetic evidence for dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR.
A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents.
Weidmann
Manfred
M
Unit for Microbiological Preparedness and Emerging Infectious Diseases, University Hospital, Stefan-Meier-Str. 19, 79014 Freiburg, Germany. manfred.weidmann@uniklinik-freiburg.de
Schmidt
P
P
Vackova
M
M
Krivanec
K
K
Munclinger
P
P
Hufert
F T
FT
D000818:Animals; D003411:Arvicolinae; D006476:Orthohantavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000662:veterinary* / Q000821:virology; D051379:Mice; D008969:Molecular Sequence Data; D009115:Muridae / Q000821:virology*; D019105:Random Amplified Polymorphic DNA Technique; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012376:Rodent Diseases / Q000635:transmission* / Q000821:virology; D017422:Sequence Analysis, DNA; D019914:Taq Polymerase / Q000378:metabolism
D019914:Taq Polymerase