Genetic structure of Atriplex halimus populations in the Mediterranean Basin.
OBJECTIVE
The saltbush Atriplex halimus is a chenopodiaceous plant well adapted to dry saline habitats and widely distributed in the Mediterranean Basin. A study was carried out to analyse the genetic diversity of A. halimus at the level of the Mediterranean Basin.


METHODS
To assess the intra- and interpopulational variation of A. halimus a total of 51 populations and six plants per populations was analysed with the RAPD-PCR technique. For the study of the phylogeny of the populations, 21 samples of A. halimus and seven samples of other species of Atriplex were analysed by the sequencing of the ITS (internal transcribed spacer) region of the ribosomal DNA.


RESULTS
The AMOVA analysis of the RAPD results showed that populations were divided into two discrete genetic groups, as the variation among groups accounted for 54.36 % of the total variance of the collection. At the same time, the intrapopulational diversity was high, as 301 out of 306 plants analysed constituted an individual RAPD haplotype. The sequencing of the ITS region also showed a significant separation of the two genetic groups, with a genetic distance of 0.023 nucleotide substitutions per site. Using A. breweri, A. canescens, A. glauca and A. prostrata as outgroups in the phylogenetic analysis, A. breweri and A. canescens are the species closest to A. halimus from this group, while A. prostrata is the most distant.


CONCLUSIONS
The present work indicates that two genetic groups of A. halimus can be distinguished after analysing the genetic diversity of 51 populations from ten countries in the Mediterranean Basin.
Ortíz-Dorda
J
J
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor sn., 30150 La Alberca (Murcia), Spain.
Martínez-Mora
C
C
Correal
E
E
Simón
B
B
Cenis
J L
JL
D000704:Analysis of Variance; D027466:Atriplex / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D008522:Mediterranean Sea; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique