title: The large scale generation of dendritic cells for the immunization of patients with non-small cell lung cancer (NSCLC).
abstract: In the current study, we generated large numbers of dendritic cells (DCs) from patients with non-small cell lung cancer (NSCLC) for a vaccine trial. The DCs were generated from CD14+ cells obtained by immuno-magnetic bead column separation technique. The CD14+ cells were placed in culture in the presence of granulocyte macrophage colony stimulating factor (GMCSF) and Interleukin 4 (IL-4). At Day 7, apoptotic bodies derived from an allogeneic NSCLC line 1650-TC were added to the cultures at a DC:tumor cell ratio of 1:1. At Day 8, the DCs were harvested, washed and injected intradermally into patients. Using this protocol we have prepared DCs for 16 patients. An average of 9.3 x 10(7) DCs was injected for the priming dose and 8.2 x 10(7) DCs for the boost. Clinical evaluation of the patients and immune assessment are presented in a separate report. The current report provides evidence for the large scale production of functional DCs derived from patients with NSCLC which can be used as vaccines in clinical trials.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky School of Medicine, Markey Cancer Center, 800 Rose Street, Lexington, Kentucky 40536, USA. Jyann1@email.uky.edu
authors.forename: John R
authors.initials: JR
authors.lastname: Yannelli
authors.forename: Jamie
authors.initials: J
authors.lastname: Sturgill
authors.forename: Terry
authors.initials: T
authors.lastname: Foody
authors.forename: Edward
authors.initials: E
authors.lastname: Hirschowitz
mesh_terms: D017209:Apoptosis; D019496:Cancer Vaccines; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology* / Q000517:prevention & control*; D018929:Cell Culture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006650:Histocompatibility Testing; D006801:Humans; D018189:Immunomagnetic Separation; D015847:Interleukin-4 / Q000494:pharmacology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology* / Q000517:prevention & control*; D010641:Phenotype
chemical_list: D019496:Cancer Vaccines; D018950:Lipopolysaccharide Receptors; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor