title: Nanostructured electrode based on multi-wall carbon nanotubes/Pt microparticles nanocomposite for electrochemical determination of thiols in rat striatum by high performance liquid chromatography separation.
abstract: In this paper, multi-wall carbon nanotubes (MWNTs)/Pt microparticles nanocomposite was prepared by electrodepositing Pt microparticles onto the MWNTs matrix. The surface of glassy carbon electrode was modified with this kind of nanocomposite for measurement of thiols, such as L-cysteine (L-Cys) and glutathione (GSH). Compared with the MWNTs or Pt microparticles modified electrode, the nanocomposite modified electrode exhibited high sensitivity and good stability for detection of thiols. According to the results of experiments, the peak currents of L-Cys and GSH are linear with their concentrations and the detection limits (S/N=3) are 2.9 x 10(-8) mol/L and 4.5 x 10(-8) mol/L, respectively. Coupled with microdialysis, the method has been successfully applied to the determination of these two thiols in rat striatal microdialysates.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Xian
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Haiting
authors.initials: H
authors.lastname: Wang
authors.forename: Lihui
authors.initials: L
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D010984:Platinum / Q000737:chemistry; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D013438:Sulfhydryl Compounds; D010984:Platinum