Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe.
Schizosaccharomyces pombe cells divide by medial fission through contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Here we describe the identification of seven genes (adg1(+), adg2(+), adg3(+), cfh4(+), agn1(+), eng1(+), and mid2(+)) whose expression is induced by the transcription factor Ace2p. The expression of all of these genes varied during the cell cycle, maximum transcription being observed during septation. At least three of these proteins (Eng1p, Agn1p, and Cfh4p) localize to a ring-like structure that surrounds the septum region during cell separation. Deletion of the previously uncharacterized genes was not lethal to the cells, but produced defects or delays in cell separation to different extents. Electron microscopic observation of mutant cells indicated that the most severe defect is found in eng1Delta agn1Delta cells, lacking the Eng1p endo-beta-1,3-glucanase and the Agn1p endo-alpha-glucanase. The phenotype of this mutant closely resembled that of ace2Delta mutants, forming branched chains of cells. This suggests that these two proteins are the main activities required for cell separation to be completed.
Alonso-Nuñez
Maria Luisa
ML
Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, CSIC/Universidad de Salamanca, Campus Miguel de Unamuno, Spain.
An
Hanbing
H
Martín-Cuadrado
Ana Belén
AB
Mehta
Sapna
S
Petit
Claudia
C
Sipiczki
Matthias
M
del Rey
Francisco
F
Gould
Katheleen L
KL
de Aldana
Carlos R Vázquez
CR
D002453:Cell Cycle; D002455:Cell Division; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D008938:Mitosis; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
C502932:Ace2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors