[A preliminary investigation on the occurrence of protozoa in swimming pools in Italy].
In Italy controls on the hygienic quality of swimming pools are fixed by the Accordo tra il Ministero della Salute, le regioni e le province autonome di Trento e di Bolzano, come into force in 2003. In the present study swimming pools were investigated from the microbiological point of view on the basis of the new legislation. Contemporaneously, for the first time in Italy, analytical controls on the occurrence of the protozoa Cryptosporidium and Giardia were performed with both techniques of immunofluorescence and polymerase chain reaction. Results evidenced a good water quality when the bacterial parameters stated by law were considered. However in some swimming pools, the protozoa were also recovered. Factors affecting the use of molecular techniques on the analysis of environmental samples are also put into evidence.
Bonadonna
L
L
Dipartimento Ambiente e Connessa Prevenzione Primaria, Istituto Superiore di Sanità, Roma. lucybond@iss.it
Briancesco
R
R
Magini
V
V
Orsini
M
M
Romano-Spica
V
V
D000818:Animals; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis; D056890:Eukaryota / Q000235:genetics / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D005872:Giardia / Q000235:genetics / Q000302:isolation & purification; D007558:Italy; D016133:Polymerase Chain Reaction; D013551:Swimming Pools / Q000592:standards*; D014867:Water / Q000469:parasitology*; D014871:Water Microbiology / Q000592:standards
D016054:DNA, Protozoan; D014867:Water