[Effect of hypoxia on energy metabolism of G1/S renal epithelial cells of piglets].
OBJECTIVE
To determine whether effect of energy metabolism on G1/S renal tubular epithelial cells (RTEs) is strongly associated with anoxemia.


METHODS
Neonatal pig's RTEs were synchronized by the Excess Thymidine Block technique and the distribution of G1/S phases was determined by flow cytometry (FACS). The hypoxic-cell model of G1-phase RTEs was established by exposure of the RTEs to sodium cyanide (NaCN), and the preventive (NaCN+Cantharidin) group and un-treated control group were set up at the same time. The ATP levels in three RTEs groups were measured by high-performance liquid chromatography.


RESULTS
FACS measurements revealed that (99.52 +/- 0.83) % of the neonatal RTEs were at G1/S transition. The concentration of ATP in the RTEs of hypoxic group at 0, 60, 120 minutes after with NaCN treatment for one hour was higher than that of the preventive group and un-treated control group (P<0.05). At 180 minutes after NaCN treatment, the RTEs' ATP in hypoxic group was significantly reduced as compared with those of the preventive and un-treated control groups (P<0.05).


CONCLUSIONS
Abnormal energy metabolism in newborn piglet's RTEs is significantly associated with anoxia that may further interfere with the advance of RTEs' cell cycle and Cantharidin can regulate the ATP metabolism of hypoxic RTEs.
Yao
Yu-jia
YJ
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Shen
Qing
Q
Xiong
Ying
Y
D000818:Animals; D000831:Animals, Newborn; D002193:Cantharidin / Q000494:pharmacology; D015687:Cell Hypoxia; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D013552:Swine
D002193:Cantharidin; D005947:Glucose