Dynamic modification of microorganisms by pyrenebutanoate for fluorometric detection in capillary zone electrophoresis.
Pyrenebutanoate, a fluorescent amphiphilic probe, is suggested here as a capillary zone electrophoresis (CZE) buffer additive for dynamic modification and analysis of microbial cells. Mixed cultures of microorganisms Escherichia coli, Candida albicans, Enterococcus faecalis and Staphylococcus epidermidis were concentrated, resolved by CZE and detected. Using UV excitation for on-column fluorometric detection, a detection sensitivity for the microorganisms on the order of from one to tens of injected cells was achieved.
Horká
Marie
M
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno. horka@iach.cz
Růzicka
Filip
F
Holá
Veronika
V
Slais
Karel
K
D002176:Candida albicans / Q000187:drug effects / Q000302:isolation & purification*; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000302:isolation & purification*; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D005470:Fluorometry; D011721:Pyrenes / Q000494:pharmacology*; D015203:Reproducibility of Results; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000494:pharmacology*
D004573:Electrolytes; D005456:Fluorescent Dyes; D011721:Pyrenes; D013501:Surface-Active Agents; C002218:1-pyrenebutyrate