[Investigation on extraction conditions of tebufenozide residue from cabbage using supercritical fluid extraction].
An extraction method of tebufenozide using supercritical fluid extraction (SFE) and high performance liquid chromatography (HPLC) was developed. The selected conditions were 48.3 MPa (7000 psi), 60 degrees C, 20 min of static time, dynamic extraction with 10 mL of CO2, 0.04 mL/g of methanol as static modifier, and 5 mL of acetone as collecting solvent. Under these conditions, the recovery of tebufenozide extracted by SFE was 100.75%. The extract was analyzed directly by HPLC with a photodiode array detector. The chromatographic conditions were: UV detection wavelength, 245 nm; column, a C18 column; mobile phase, acetonitrile-water (55:45, v/v); flow rate, 1.0 mL/min; injection volume, 5 microL.
Yang
Lirong
L
Biorational Pesticide Research and Service Center, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China. lirongyang@163.com
Zhang
Xing
X
Chen
Anliang
A
Li
Guangze
G
D001937:Brassica / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D006834:Hydrazines / Q000032:analysis*; D007202:Indicators and Reagents; D007306:Insecticides / Q000032:analysis*; D011312:Pressure; D013696:Temperature
D006834:Hydrazines; D007202:Indicators and Reagents; D007306:Insecticides; C082026:tebufenozide