Kinetic spectrofluorimetric determination of trace ascorbic acid based on its inhibition on the oxidation of pyronine Y by nitrite.
A highly sensitive spectrofluorimetric method is proposed for the determination of trace amount of ascorbic acid using a new indication. The method is based on the inhibition of ascorbic acid on the oxidation of pyronine Y (PRY) by nitrite. The detection limit for ascorbic acid is 0.012 microg ml(-1), the linear range of the determination is 0.02-0.36 microg ml(-1). Analytical parameters, such as reagent concentration, pH, reaction temperature and time, were optimized. The relative standard deviations of eleven replication determinations of 0.12 and 0.24 microg ml(-1) ascorbic acid were 1.4 and 0.72%, respectively. This method has been used to determine ascorbic acid in pharmaceuticals, vegetables, fruits and soft drink with satisfactory results.
Feng
Suling
S
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Wang
Jin
J
Chen
Xingguo
X
Fan
Jing
J
D001205:Ascorbic Acid / Q000032:analysis* / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D011754:Pyronine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013464:Sulfuric Acids / Q000378:metabolism; D013997:Time Factors
D009573:Nitrites; D013464:Sulfuric Acids; C033158:sulfuric acid; D001205:Ascorbic Acid; D011754:Pyronine