[Experimental gene therapy of keloid in vitro using recombinant adenovirus coding for Fas gene].
OBJECTIVE
To replace dysfunctional Fas gene and reconstruct the blocked Fas signal by using two kinds of prepared recombinant Adenovirus which have human Fas gene .


METHODS
After the keloids derived from fibroblasts were infected by the Adenovirus, the expressions of Fas protein before the exposure and after the exposure was compared. Then the function of the newly produced Fas protein was detected.


RESULTS
The highly improve expression of Fas protein in the infected keloid derived fibroblasts was detected. Obvious apoptosis was also detected in the infected keloid derived from fibroblasts under the condition of exposing to FasMcab.


CONCLUSIONS
The recombinant Adenovirus with Fas gene can transfect the Fas gene into keloid-derived fibroblasts and highly improved the expression of Fas protein. The newly expressed Fas gene can reconstruct the blocked Fas signal. Ad-Fas (B) has better therapeutic effect in vitro gene therapy. The correlation between keloid and Fas gene was further proved and it may pave the way for further gene therapy in keloid.
Lu
Feng
F
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University (the First Military Medical University), Guangzhou Guangdong 510515, P R China.
Gao
Jianhua
J
D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D007627:Keloid / Q000235:genetics / Q000473:pathology / Q000628:therapy; D008856:Microscopy, Fluorescence; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D014162:Transfection; D019014:fas Receptor / Q000235:genetics / Q000502:physiology*
D019014:fas Receptor