Structure of the O-polysaccharide and serological studies of the lipopolysaccharide of Proteus penneri 60 classified into a new Proteus serogroup O70.
An alkali-treated lipopolysaccharide of Proteus penneri strain 60 was studied by chemical analyses and 1H, 13C and 31P NMR spectroscopy, and the following structure of the linear pentasaccharide-phosphate repeating unit of the O-polysaccharide was established: 6)-alpha-D-Galp-(1-->3)-alpha-L-FucpNAc-(1-->3)-alpha-D-GlcpNAc-(1-->3)-beta-D-Quip4NAc-(1-->6)-alpha-D-Glcp-1-P-(O--> Rabbit polyclonal O-antiserum against P. penneri 60 reacted with both core and O-polysaccharide moieties of the homologous LPS. Based on the unique O-polysaccharide structure and serological data, we propose to classify P. penneri 60 into a new, separate Proteus serogroup O70. A weak cross-reactivity of P. penneri 60 O-antiserum with the lipopolysaccharide of Proteus vulgaris O8, O15 and O19 was observed and discussed in view of the chemical structures of the O-polysaccharides.
Zych
Krystyna
K
Department of General Microbiology, Institute of Microbiology and Immunology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Perepelov
Andrei
A
Baranowska
Agata
A
Zabłotni
Agnieszka
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Sidorczyk
Zygmunt
Z
D000818:Animals; D000907:Antibodies, Bacterial; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D044095:Proteus penneri / Q000737:chemistry* / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity; D011817:Rabbits; D012703:Serotyping
D000907:Antibodies, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D019081:O Antigens