title: From drug to protein: using yeast genetics for high-throughput target discovery.
abstract: The budding yeast Saccharomyces cerevisiae has long been an effective eukaryotic model system for understanding basic cellular processes. The genetic tractability and ease of manipulation in the laboratory make yeast well suited for large-scale chemical and genetic screens. Several recent studies describing the use of yeast genetics for high-throughput drug target identification are discussed in this review.
authors.affiliation: Rosetta Inpharmatics LLC, Merck & Co., Inc., 401 Terry Avenue North, Seattle, Washington 98109, USA. christopher_armour@merck.com
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Armour
authors.forename: Pek Yee
authors.initials: PY
authors.lastname: Lum
mesh_terms: D015195:Drug Design; D023281:Genomics / Q000379:methods* / Q000639:trends; D011506:Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D013678:Technology, Pharmaceutical / Q000379:methods; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins