Characterization of the leukemia inhibitory factor receptor in the goldfish (Carassius auratus).
The cytokine leukemia inhibitory factor (LIF) and its receptor LIFR have been extensively characterized in mammals. LIF has been shown to mediate the proliferation, differentiation and activation of a number of cell types in various tissues. This paper reports on the identification of a novel LIFR isolated from goldfish (Carassius auratus) macrophages. Goldfish LIFR shares a 26% amino acid sequence identity with mammalian LIFR sequences; however it retains all of the conserved amino acid motifs that identify a functional LIFR such as the cytokine binding domains and the box-1 and box-2 motifs. The goldfish LIFR phylogenetically groups with the other identified LIFRs from human, mouse, rat and chicken, and it appears to be ancestral to the divergence of the oncostatin M receptor (OSMR). The tissue expression of goldfish LIFR is observed in the gill, kidney and brain as well as sorted goldfish macrophages which exhibit higher expression than monocytes and early progenitor cells.
Hanington
Patrick C
PC
Department of Biological Sciences, Room CW-405, University of Alberta, Edmonton, AB, Canada T6G 2E9.
Belosevic
Miodrag
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D017931:DNA Primers; D015723:Gene Library; D005880:Gills / Q000378:metabolism; D006054:Goldfish / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008264:Macrophages / Q000378:metabolism; D008969:Molecular Sequence Data; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism; D053606:Receptors, OSM-LIF; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D017931:DNA Primers; C507840:LIFR protein, human; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507841:Lifr protein, mouse; C507842:Lifr protein, rat; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF