Effect of glutamine and spray-dried plasma on growth performance, small intestinal morphology, and immune responses of Escherichia coli K88+-challenged weaned pigs.
Forty weaned barrows (5.32 +/- 0.3 kg BW) at 17 +/- 2 d of age were used to investigate the effects of feeding glutamine and spray-dried plasma on the growth performance, small intestinal morphology, and immune responses of Escherichia coli K88-challenged pigs. Pigs were allotted to four treatments including: 1) nonchallenged control (NONC); 2) challenged control (CHAC); 3) 7% (as-fed basis) spray-dried plasma (SDP); and 4) 2% (as-fed basis) glutamine (GLN). On d 11 after weaning, all pigs were fitted with an indwelling jugular catheter. On d 12 after weaning, pigs in the CHAC, SDP, and GLN groups were orally challenged with skim milk E. coli K88 culture, whereas pigs in the NONC group were orally inoculated with sterilized skim milk. Rectal temperatures and fecal diarrheic scores were recorded and blood samples collected at 0 (baseline), 6, 12, 24, 36, and 48 h after the challenge for serum hormone and cytokine measurements. At 48 h postchallenge, all pigs were killed for evaluation of small intestinal morphology. There was no effect of feeding SDP or GLN on growth performance during the 11-d prechallenge period (P = 0.13). At 48 h after the challenge, CHAC pigs had decreased ADG (P = 0.08) and G:F (P = 0.07) compared with the NONC pigs; however, SDP and NONC pigs did not differ in G:F, and GLN and NONC pigs did not differ for ADG and G:F. At 6, 36, and 48 h after the challenge, CHAC, SDP, and GLN pigs had increased rectal temperature relative to the baseline (P = 0.09). At 12 and 36 h after the challenge, CHAC pigs had the highest incidence of diarrhea among treatments (P = 0.08). Serum IL-6 and ACTH were not affected by treatment or time after E. coli challenge (P = 0.11). In proximal, midjejunum, and ileum, CHAC pigs had greater villous atrophy and intestinal morphology disruption than NONC pigs (P < 0.01), whereas SDP and GLN pigs had mitigated villous atrophy and intestinal morphology impairment after E. coli challenge. Pigs in the SDP had the lowest GH at 12 h and the greatest GH at 36 h after the challenge among treatments (P = 0.08). Pigs in the NONC had the highest IGF-1 at 12 and 36 h postchallenge (P < 0.04). These results indicate that feeding glutamine has beneficial effects in alleviating growth depression of E. coli K88-challenged pigs, mainly via maintaining intestinal morphology and function, and/or possibly via modulating the somatotrophic axis.
Yi
G F
GF
Department of Animal Science, University of Missouri, Columbia 65211, USA.
Carroll
J A
JA
Allee
G L
GL
Gaines
A M
AM
Kendall
D C
DC
Usry
J L
JL
Toride
Y
Y
Izuru
S
S
D000324:Adrenocorticotropic Hormone / Q000097:blood / Q000187:drug effects; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001831:Body Temperature; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000276:immunology; D005243:Feces; D036781:Fimbriae Proteins / Q000276:immunology; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D006128:Growth / Q000187:drug effects; D013006:Growth Hormone / Q000097:blood / Q000187:drug effects; D007107:Immune System / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects*; D008297:Male; D010949:Plasma / Q000378:metabolism; D011897:Random Allocation; D013552:Swine / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D013997:Time Factors; D014886:Weaning
D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D005973:Glutamine; D036781:Fimbriae Proteins; D007334:Insulin-Like Growth Factor I; D000324:Adrenocorticotropic Hormone; D013006:Growth Hormone