title: [Transcriptional and electron microscopic analysis of extracellular matrix proteoglycans in acute acetaminophen intoxication].
abstract: Acetaminophen (APAP) OTC regimen is not compatible with kidney and liver toxic effects. In order to further understand the determinism of the repair process, we have studied the expression of some target extracellular matrix proteoglycan components in an animal model.
  
  
  METHODS
  An acute APAP toxic dose (800 mg/bw) was administered to 45 mice aged 1 month. At 24 hours, tissue samples were processed for light microscopy, electromicroscopy and RT-PCR analysis.
  
  
  RESULTS
  Extensive necrotic lesions involving hepatocytes and tubular epithelial cells associate strong positivity for proteoglycans, detected as convoluted filaments with lateral projections, frequently associated with collagen fibers, cell surface and other matrix components. Overexpression for perlecan, syndecan 1 and 4 and low expression for biglycan was assessed by RT-PCR.
  
  
  CONCLUSIONS
  Extracellular matrix and cell surface components are early involved in both lesional mechanism and repair reaction post acetaminophen injury.
authors.affiliation: Institutul de Sănătate Publică Iaşi, Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi, Facultatea de Medicină.
authors.forename: Laurette Graziella
authors.initials: LG
authors.lastname: Cozma
authors.forename: Ioana Dana
authors.initials: ID
authors.lastname: Alexa
authors.forename: Gioconda
authors.initials: G
authors.lastname: Dobrescu
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D058578:Biglycan; D056486:Chemical and Drug Induced Liver Injury; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans / Q000032:analysis; D008107:Liver Diseases / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D023421:Models, Animal; D011509:Proteoglycans / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans
chemical_list: D018712:Analgesics, Non-Narcotic; C546885:Bgn protein, mouse; D058578:Biglycan; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508242:Sdc1 protein, mouse; C508306:Sdc4 protein, mouse; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans; C071927:perlecan; D000082:Acetaminophen