[Study on multipotential differentiation of human bone marrow mesenchymal stem cells in vitro].
OBJECTIVE
To study the capability of human bone marrow mesenchymal stem cells (MSCs) to differentiate into adipocyte, osteoblasts, and neurocytes under different experimental conditions in vitro.


METHODS
The induced human bone marrow MSCs were examined by cytochemistry staining and immunohistochemistry staining.


RESULTS
Induced human bone marrow MSCs formed alkaline phosphatase-positive aggregates and Von Kossa stain-positive nodules under the condition of osteogenic differentiation. Under the condition of adipogenic differentiation, the isolated human bone marrow MSCs formed oil red-O-positive cells. Immunohistochemistry results showed that differentiated human bone marrow MSCs expressed neuron-specific enolase (NSE) and neurofilament (NF-M).


CONCLUSIONS
Human bone marrow MSCs have the capability of multipotential differentiation in vitro.
Chang
Ying
Y
Department of Neurology Sino-Japanese Friendship Hospital, Jilin university, Jilin 130031, Jilin, China.
Qi
Xin
X
Bu
Li-sha
LS
Xu
Zhong-xin
ZX
D017667:Adipocytes / Q000166:cytology; D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000166:cytology*