[Preparation and stability of recombinant human tumor necrosis factor-alpha-loaded stealth nanoparticles].
OBJECTIVE
To prepare recombinant human tumor necrosis factor-alpha (rHuTNF-alpha) -loaded stealth nanoparticles with different PEG chain lengths and sizes, and investigate the stability of nanoparticle suspensions.


METHODS
The poly( MePEG cyanoacrylate-co-hexadecyl cyanoacrylate) (MePEG-PHDCA) and poly(hexadecyl cyanoacrylate) (PHDCA) were synthesized and characterized with Fourier transform infrared spectrum (FTIR), 1HNMR, 13CNMR and gel permeation chromatography (GPC). Uniform design was used to optimize the entrapment efficiency. The nanoparticle suspensions were stored at 2 - 8 degrees C for 4 weeks, and the particle size evolution was studied.


RESULTS
FTIR, 1HNMR and 13CNMR were consistent with the structures of MePEG-PHDCA and PHDCA whose polydispersity indexes were all less than 1.1, indicating narrow distributions. The entrapment efficiency of all nanoparticles was satisfactory. The three different mean diameters of MePEG-PHDCA and PHDCA nanoparticles were about 80 nm, 170 nm and 240 nm, separately. The nanoparticle suspensions maintained their sizes at 2 - 8 degrees C for 4 weeks


CONCLUSIONS
MePEG-PHDCA with three different molecular weight MePEG and PHDCA were synthesized successfully. There are negligible aggregations and bulk or surface erosion as for both stealth MePEG-PHDCA and conventional PHDCA nanoparticles in distilled water.
Fang
Chao
C
Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, 200032.
Shi
Bin
B
Pei
Yuan-Ying
YY
D003487:Cyanoacrylates / Q000737:chemistry*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004355:Drug Stability; D006801:Humans; D036103:Nanotechnology; D011092:Polyethylene Glycols / Q000737:chemistry*; D011994:Recombinant Proteins / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage*
D003487:Cyanoacrylates; D004337:Drug Carriers; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C121140:polyethyleneglycol polyhexadecylylcyanoacrylate copolymer; D011092:Polyethylene Glycols