[Structural diversity of O-polysaccharides and serological classification of Pseudomonas syringae pv. garcae and other strains of genomospecies 4].
Novel O-serotypes were revealed among Pseudomonas syringae pv. garcae strains by using a set of mouse monoclonal antibodies specific to the lipopolysaccharide O-polysaccharide. Structural studies showed that the O-polysaccharide of P. syringae pv. garcae NCPPB 2708 is a hitherto unknown linear L-rhamnan lacking strict regularity and having two oligosaccharide repeating units I and II, which differ in the position of substitution in one of the rhamnose residues and have the following structures: I: --> 3)-alpha-L-Rha-(1 --> 2)-alpha-L-Rha-(1 --> 2)-alpha-L-Rha-(1 --> 3)-alpha-L-Rha-(1 -->; II: --> 3)-alpha-L-Rha-(1 --> 3)-alpha-L-Rha-(1 --> 2)-alpha-L-Rha-(1 --> 3)-alpha-L-Rha-(1 -->. The branched polysaccharides of P. syringae pv. garcae ICMP 8047 and NCPPB 588 have the same L-rhamnan backbone with repeating units I and II and a lateral chain of (alpha1 --> 4)- or (alpha1 --> 3)-linked residues of 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc). Several monoclonal antibody epitopes associated with the L-rhamnan backbone or the lateral alpha-D-Fuc3NAc residues were characterized.
Ovod
V V
VV
Zdorovenko
E L
EL
Shashkov
A S
AS
Kocharova
N A
NA
Knirel'
Iu A
IuA
D000116:Acetylgalactosamine / Q000031:analogs & derivatives; D002240:Carbohydrate Sequence; D000939:Epitopes; D005643:Fucose / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000276:immunology; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D044224:Pseudomonas syringae / Q000737:chemistry / Q000145:classification* / Q000276:immunology; D012210:Rhamnose / Q000276:immunology; D012703:Serotyping
C478186:3-acetamido-3,6-dideoxy-D-galactose; D000939:Epitopes; D009005:Monosaccharides; D019081:O Antigens; D005643:Fucose; D000116:Acetylgalactosamine; D012210:Rhamnose