Perfusion pressure in kidneys of arthritic rats and the influence of L-NAME.
Kidneys of control and arthritic rats were perfused with a hemoglobin-free perfusion fluid with simultaneous monitoring of perfusion pressure and oxygen uptake. The basal values of renal perfusion pressure were 76.3 +/- 4.63 and 59.96 +/- 3.65 mm Hg in control and arthritic rats, respectively. Infusion of 100 microM Nomega-nitro-L-arginine methyl ester (L-NAME) an inhibitor of constitutive and inducible nitric oxide synthase, increased the renal perfusion pressure to 91.6 +/- 5.52 and 106.69 +/- 8.47 mm Hg in control and arthritic rats, respectively. Oxygen uptake of kidneys from control rats was 2.48 +/- 0.74 micromol min(-1) g(-1) and that of the arthritic rats was 2.44 +/- 0.75 micromol min(-1) g(-1). These results are consistent with an increased production of hemodynamically active nitric oxide in kidneys of arthritic rats.
Taguchi
Willian S
WS
Laboratory of Liver Metabolism, Department of Biochemistry, University of Maringá, Brazil.
Comar
Jurandir F
JF
Fagundes
Djalma J
DJ
Bracht
Adelar
A
Suzuki-Kemmelmeier
Fumie
F
D000818:Animals; D001168:Arthritis / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester