title: Cytotoxic pyranonaphthoquinones from Melloa quadrival vis (Bignoniaceae).
abstract: Three new pyranonaphthoquinones: 5-hydroxy-6-methoxy-alpha-lapachone, 5,6-dihydroxy-a-lapachone and 4',5-dihydroxy-6-methoxy-alpha-lapachone, and two known compounds: lapachol and 5,5'-dihydroxy-3',4',7-trimethoxyflavanone, were isolated from the stem bark of Melloa quadrivalvis. Their structures were established by spectrometric data, mainly 1D- and 2D-NMR and mass spectra. The methylazoetetrazolium (MTT) method using viable cells of the strain Hep2 and the strain NCIH-292 demonstrated cytotoxic activity. The CI50 was also calculated. The chloroform extract and 5-hydroxy-6-methoxy-alpha-lapachone inhibited cell growth.
authors.affiliation: Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, 50740-521, Recife, PE, Brazil.
authors.forename: Cláudia S De Andrade
authors.initials: CS
authors.lastname: Lima
authors.forename: Elba L C
authors.initials: EL
authors.lastname: De Amorim
authors.forename: Silene C
authors.initials: SC
authors.lastname: Nascimento
authors.forename: Christiane F
authors.initials: CF
authors.lastname: De Araújo
authors.forename: Maria F
authors.initials: MF
authors.lastname: Agra
authors.forename: José M
authors.initials: JM
authors.lastname: Barbosa-Filho
authors.forename: Marcelo S
authors.initials: MS
authors.lastname: Silva
authors.forename: Emídio V L
authors.initials: EV
authors.lastname: Da-Cunha
authors.forename: Ivo J Curcino
authors.initials: IJ
authors.lastname: Vieira
authors.forename: Raimundo
authors.initials: R
authors.lastname: Braz-Filho
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D029402:Bignoniaceae / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D004354:Drug Screening Assays, Antitumor; D008113:Liver Neoplasms / Q000473:pathology; D009285:Naphthoquinones / Q000302:isolation & purification* / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: C499952:4',5-dihydroxy-6-methoxy-alpha-lapachone; C499950:5,6-dihydroxy-alpha-lapachone; C499951:5-hydroxy-6-methoxy-alpha-lapachone; D000972:Antineoplastic Agents, Phytogenic; D009285:Naphthoquinones; D010936:Plant Extracts; C008252:lapachol