[Chiral separation of 3-phenyllactic acid by capillary electrophoresis].
Various factors affecting the chiral resolution of 3-phenyllactic acid by capillary zone electrophoresis such as the type of cyclodextrin, concentration of the chiral selector, buffer pH, voltage, and temperature were studied and optimized. Using 30 mmol/L hydroxypropyl-beta-cyclodextrin as the chiral selector, and 0.1 mol/L phosphate solution (pH 5.5) as the running buffer, the baseline separation of 3-phenyllactic acid enantiomers was achieved with a resolution factor of 1.51. The separation voltage was 26 kV and the temperature was 25 degrees C.
Li
Demao
D
College of Technology and Engineering, South China University of Tropical Agriculture, Danzhou 571737, China.
Li
Congfa
C
Liu
Sixin
S
Chen
Limei
L
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry
D002021:Buffers; D007773:Lactates; D047392:beta-Cyclodextrins; C017648:3-phenyllactic acid; D000073738:2-Hydroxypropyl-beta-cyclodextrin