Rapid and simple quantification of bacterial cells by using a microfluidic device.
This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.
Sakamoto
Chieko
C
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Yamaguchi
Nobuyasu
N
Nasu
Masao
M
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation*; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D016958:Pseudomonas putida / Q000302:isolation & purification*; D045483:Rivers / Q000382:microbiology; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D003470:Culture Media; D009930:Organic Chemicals; C098798:SYBR Green II