17beta-Estradiol inhibits calcium-dependent, but not calcium-independent, contraction in isolated rat aorta.
It is now well known that 17beta-estradiol has an endothelium-independent, non-genomic vasorelaxant effect. We hypothesized that 17beta-estradiol has its non-genomic effect on calcium-independent contraction in de-endothelialized rat aortic rings. Rat aortic ring preparations were mounted in organ baths and exposed to contractile agents. 17beta-Estradiol (8, 20 or 50 microM), but not 17alpha-estradiol, concentration-dependently decreased the tension induced by 1.0 microM phenylephrine (PE) in the presence, but not in the absence, of calcium in the solution. Pretreatment with 17beta-estradiol concentration-dependently inhibited vascular contractions induced by cumulative addition of PE or calcium and almost completely abolished those induced by cumulative addition of Bay K8644, a calcium channel opener. Furthermore, 17beta-estradiol also concentration-dependently decreased the tension induced by 0.3 microM phorbol 12,13-dibutyrate (PDBu), a protein kinase C activator, in the presence of calcium in the solution, but not in the absence of calcium in the solution. Pretreatment with 17beta-estradiol had little effect on vascular contractions induced by PDBu or PE or on PE-induced mitogen-activated protein kinase (MAPK) activation in calcium-free Krebs solution. These results suggest that 17beta-estradiol inhibits calcium-dependent, but not calcium-independent, vascular contraction.
Lee
Hae-Ahm
HA
Department of Physiology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea.
Seong
Yujin
Y
Lee
Won-Jung
WJ
Kim
Inkyeom
I
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D002120:Calcium Channel Agonists; D014662:Vasoconstrictor Agents; D010656:Phenylephrine; D004958:Estradiol; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D002118:Calcium