title: Microscopy and cytochemistry of the biogenesis of the parasitophorous vacuole.
abstract: Some parasitic protozoa are able to penetrate into host cells where they multiply. The process of penetration involves steps such as attachment to the host cell surface, internalization of the protozoan through an endocytic process with the formation of a parasitophorous vacuole (PV), and the subsequent interaction of the protozoan with the membrane lining the PV. This review analyzes the biogenesis of the PV from a morphological and cytochemical perspective. Special emphasis is given to (a) the localization of plasma membrane-associated enzymes such as Na(+)-K(+)-ATPase, Ca(2+)-ATPase, 5'-nucleotidase, and NAD(P)H-oxidase, (b) glycoconjugates, detected using labeled lectins, (c) anionic sites, detected using cationic particles, and (d) integral membrane proteins, using freeze-fracture replicas, and lipids during the formation of the PV containing Trypanosoma cruzi, Leishmania, Toxoplasma gondii, and Plasmodium.
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, CCS-Bloco G, 21941-900, Rio de Janeiro, Brazil. wsouza@biof.ufrj.br
authors.forename: Wanderley
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D056890:Eukaryota / Q000378:metabolism* / Q000648:ultrastructure*; D005614:Freeze Fracturing; D006651:Histocytochemistry; D006790:Host-Parasite Interactions; D008565:Membrane Proteins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D014617:Vacuoles / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D008565:Membrane Proteins; D000251:Adenosine Triphosphatases