Amphoteric, buffering chromatographic beads for proteome prefractionation. I: theoretical model.
The possibility is reported here of fractionating proteins on amphoteric, buffering resins via ion-exchange chromatography. A given protein's adsorption to a particular amphoteric buffering resin is characterized by a bell-shaped curve in which the maximum protein binding capacity is observed at an optimum pH value lying approximately midway between the isoelectric point values (pI) of the resin and the protein. On either side of this maximum the protein binding capacity declines steadily, reaching zero at the pI of either the protein or exchanger. For instance, on beads of pI equal to 8, four proteins, two acidic (bovine albumin and ovalbumin) and two basic (cytochrome c and lysozyme), exhibit binding curves reaching zero values for the whole set when the exchanger is conditioned at pH 8.0. Away from the pI, and on both sides of the pH scale, the bell-shaped adsorption curves reach a maximum, for each protein, at a pH located at the midpoint between the pI values of each protein and that of the exchanger, and decline steadily to reach zero at the pI value of each protein species. Separation of model proteins using different amphoteric buffering resins of various pI was possible at different pH values according to both the pI of the proteins and of the exchangers. It was also demonstrated, using surface enhanced laser desorption/ionization mass spectrometry and two dimensional electrophoretic mapping, that separation of an Escherichia coli cell lysate on columns packed with amphoteric buffering resins of different pI and titrated to a particular pH value, delivered two distinctly different fractions, i.e. characteristically composed of, on the one hand, proteins having a pI below the buffer pH (the 'adsorbed' fraction), and on the other, of alkaline proteins possessing a pI above the pH of the buffer (the 'unadsorbed' fraction). This approach represents an attractive addition and/or alternative to the armory of protein pre-fractionation techniques currently employed in proteomics.
Fortis
Frédéric
F
Ciphergen Biosystems-Biosepra, Cergy-Pontoise, France.
Girot
Pierre
P
Brieau
Olivier
O
Boschetti
Egisto
E
Castagna
Annalisa
A
Righetti
Pier Giorgio
PG
D000418:Albumins / Q000302:isolation & purification; D002021:Buffers; D002852:Chromatography, Ion Exchange; D045424:Complex Mixtures / Q000302:isolation & purification; D045304:Cytochromes c / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D029968:Escherichia coli Proteins / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D007526:Isoelectric Point; D008863:Microspheres; D008956:Models, Chemical; D009113:Muramidase / Q000302:isolation & purification; D010047:Ovalbumin / Q000302:isolation & purification; D040081:Protein Array Analysis; D020543:Proteome / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000418:Albumins; D002021:Buffers; D045424:Complex Mixtures; D029968:Escherichia coli Proteins; D007475:Ion Exchange Resins; D020543:Proteome; D010047:Ovalbumin; D045304:Cytochromes c; D009113:Muramidase