Anti-Ki-67 peptide nucleic acid affects the proliferation and apoptosis of human renal carcinoma cells in vitro.
We treated in vitro human renal carcinoma cells (cell line 786-0) with the lipid-delivered peptide nucleic acids (PNAs) against Ki-67 gene. Corresponding control groups were treated with the antisense oligonucleotides (ASOs) of the same nucleobase sequence, and with mismatched PNAs. In cells treated by anti-Ki-67 PNAs, the Ki-67 expression rate, Ki-67 protein level, cell growth and the DNA synthesis-indicative 3H-thymidine incorporation rate were lower than in the ASO-treated groups, and reduced significantly compared to untreated controls, whereas the rate of apoptosis was markedly increased by PNA treatment. We conclude that anti-Ki-67 PNA has more strong (than ASO) and dose-dependent effects on the proliferation and apoptosis of human renal carcinoma cells. Our results indicate that the strategy of using PNA against the Ki-67 gene might be a promising approach in renal carcinoma therapy.
Zheng
Jun-Nian
JN
Laboratory of Urology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China.
Sun
Ya-Feng
YF
Pei
Dong-Sheng
DS
Liu
Jun-Jie
JJ
Sun
Xiao-Qing
XQ
Chen
Jia-Chun
JC
Cai
Wei-Qi
WQ
Li
Wang
W
Cao
Jing-Yi
JY
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology; D020135:Peptide Nucleic Acids / Q000494:pharmacology*
D019394:Ki-67 Antigen; D020135:Peptide Nucleic Acids