title: Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays.
abstract: Gene expression profiles were collected from Escherichia coli strains (OST3410, TK33, and TK31) before and after exposure to organic solvents, and the six genes that showed higher gene expression were selected. Among these genes, glpC encoding the anaerobic glycerol-3-phosphate dehydrogenase subunit C remarkably increased the organic solvent tolerance.
authors.affiliation: Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shimizu
authors.forename: Shuhei
authors.initials: S
authors.lastname: Hayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kako
authors.forename: Maiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Norihiko
authors.initials: N
authors.lastname: Tsukagoshi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Doukyu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Honda
mesh_terms: D003510:Cyclohexanes / Q000494:pharmacology; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012997:Solvents / Q000494:pharmacology*; D014992:Xylenes / Q000494:pharmacology
chemical_list: D003510:Cyclohexanes; D029968:Escherichia coli Proteins; D012997:Solvents; D014992:Xylenes; D005993:Glycerolphosphate Dehydrogenase