[Real-time quantitative PCR for toxoplasmosis diagnosis].
Congenital toxoplasmosis results from foetus contamination by Toxoplasma gondii during pregnancy. It is a frequent and severe condition calling for close monitoring of mothers at risk. During the last decades, numerous advances have been made specially in the antenatal diagnosis. The congenital toxoplasmosis diagnosis relies currently on PCR test of amniotic fluid, with a sensitivity of 80%. More recently, real-time quantitative PCR has been developed to improve toxoplasmosis diagnosis. We therefore compared the diagnosis value of quantitative real-time PCR with our conventional PCR-hybridization for the diagnosis of congenital toxoplasmosis.
Ordinaire
I
I
Service de parasitologie mycologie, Hôpital Calmette, CHU de Lille.
Simon
A
A
Fréalle
E
E
Soula
F
F
Valat
A-S
AS
Rouland
V
V
Subtil
D
D
Dei-Cas
E
E
Camus
D
D
Delhaes
L
L
D000653:Amniotic Fluid / Q000469:parasitology; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic; D011296:Prenatal Diagnosis; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000635:transmission
D016054:DNA, Protozoan