Lack of receptor activator of nuclear factor-kB ligand (RANKL) expression and functional production by human multiple myeloma cells.
The direct expression and production of the critical osteoclastogenic factor, the receptor activator of NF-kB ligand (RANKL), is a matter of controversy. In this study we definitively demonstrate by both qualitative and quantitative polymerase chain reaction analysis that human myeloma cells do not express significant levels of RANKL mRNA or produce RANKL able to stimulate osteoclast formation.
Giuliani
Nicola
N
Colla
Simona
S
Morandi
Francesca
F
Barille-Nion
Sophie
S
Rizzoli
Vittorio
V
D002352:Carrier Proteins / Q000378:metabolism* / Q000502:physiology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000502:physiology*; D009101:Multiple Myeloma / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism; D020033:Protein Isoforms; D053245:RANK Ligand; D012333:RNA, Messenger / Q000378:metabolism; D053246:Receptor Activator of Nuclear Factor-kappa B; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; C505688:TNFSF11 protein, human