Conformation and dynamics of single DNA molecules in parallel-plate slit microchannels.
The conformation and diffusion of a single DNA molecule confined between two parallel plates are examined using both single molecule experiments and Brownian dynamics simulations accounting for hydrodynamic interactions. The degree of chain stretching and the diffusivity are characterized as a function of the chain confinement and the channel geometry. Good agreement is found between the simulations, experiments, and scaling theory predictions.
Chen
Y-L
YL
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53703, USA.
Graham
M D
MD
de Pablo
J J
JJ
Randall
G C
GC
Gupta
M
M
Doyle
P S
PS
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D015342:DNA Probes / Q000737:chemistry* / Q000648:ultrastructure*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D009038:Motion; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
D015342:DNA Probes; D004247:DNA