[In vitro activity of amphotericin B, itraconazole and voriconazole against 20 species of Aspergillus using the Sensititre microdilution method].
BACKGROUND
The in vitro activity of antifungal agents against Aspergillus has been recently investigated. We studied the susceptibility to amphotericin B, itraconazole and voriconazole of 68 strains belonging to 20 different Aspergillus species.


METHODS
The minimum inhibitory concentrations (MICs) for 10 strains of A. fumigatus, 9 A. flavus, 8 A. niger, 5 A. tamarii, 5 A. versicolor, 4 A. terreus, 3 A. glaucus, 3 A. ochraceus, 3 A. oryzae, 2 A. candidus, 2 A. chevalieri, 2 A. rubrobrunneus, 2 A. sclerotiorum, 2 A. sydowii, 2 A. unguis, 2 A. ustus, 1 A. clavatus, 1 A. nidulans, 1 A. pseudofischeri y 1 A. reptans, were determined using the Sensititre Yeast One microdilution method.


RESULTS
Voriconazole was more active in vitro than amphotericin B and itraconazole, with 95.6% (65/68) of strains exhibiting MICs of < or = 2 mg/l. A. flavus, A. versicolor, A. terreus, A. ochraceus, A. sclerotiorum, A. ustus and A. reptans species presented reduced susceptibility to amphotericin B (MIC > or = 2 mg/l); A. niger, A. versicolor, A. sclerotiorum and A. ustus showed in vitro resistance to itraconazole (MIC > or = 1 mg/l); and A. sclerotiorum and A. ustus displayed poor susceptibility to voriconazole (MIC > or = 2 mg/l).


CONCLUSIONS
The susceptibility of Aspergillus species to itraconazole and voriconazole was generally good; nevertheless, susceptibility to amphotericin B was low. These results suggest that Aspergillus susceptibility testing to this antifungal agent would be advisable to guide treatment.
García-Martos
Pedro
P
Servicio de Microbiología, Hospital Universitario Puerta del Mar, Cádiz, Spain. pedromartos@hotmail.com
García-Agudo
Lidia
L
Gutiérrez-Calzada
Jesús
J
Ruiz-Aragón
Jesús
J
Saldarreaga
Abel
A
Marín
Pilar
P
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000145:classification / Q000187:drug effects*; D025141:Drug Resistance, Fungal; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011743:Pyrimidines / Q000494:pharmacology*; D013045:Species Specificity; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D017964:Itraconazole; D000666:Amphotericin B; D065819:Voriconazole