title: [Effect of puerarin and daidzein on proliferating vascular smooth muscle cells].
abstract: OBJECTIVE
  To observe the different effects of Puerarin and Daidzein on the expression of proliferating vascular smooth muscle cells, and to discuss the mechanism.
  
  
  METHODS
  MT was used to detect the state of VSMC (vascular smooth muscle cell) activity. The expression levels of Survivin, Bcl-xl, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) were analyzed quantitatively by reverse transcriptase polymerase chain reaction (Rt-PCR).
  
  
  RESULTS
  Compared with Puerarin groups, VSMC activity in daidzein groups was lower, and the ratio of Bax/Gapdh/Bcl-xl/Gapdh was higher.
  
  
  CONCLUSIONS
  The inhibition effect of daidzein on VSMC proliferation is stronger than that of puerarin.
authors.affiliation: Dongzhimen Hospital, University of Traditional Chinese Medicine, Beijing 100029, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Lu-ya
authors.initials: LY
authors.lastname: Wang
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.forename: Tie-duo
authors.initials: TD
authors.lastname: Kang
mesh_terms: D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D009363:Neoplasm Proteins; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D029908:Pueraria / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077022:Survivin; D014665:Vasodilator Agents / Q000494:pharmacology; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
chemical_list: C494826:BAX protein, human; C494811:BCL2L1 protein, human; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D007529:Isoflavones; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D000077022:Survivin; D014665:Vasodilator Agents; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C004742:daidzein; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C033607:puerarin