[Effect of endophytic fungi on the culture and four enzyme activities of Anoectochillus formosanus].
OBJECTIVE
To study the effect of endophytic fungi.


METHODS
A. formosanus, harvested after having been cultured for age, statistics were taken, fresh weight and dry weight were gained, and enzyme activities of chitinase, beta-1,3-glucase, phenylalanine ammonia-lyase and polyphenoloxidase were determined.


RESULTS
The survial rates of A. formosanus inoculated with endophytic fungi was 100%. The effect of fungi on fresh weight was very significant (P < 0.01). The effect of fungi on dry weight was significant (P > 0.05). The four enzyme activities were enhanced by endophytic fungi, comparision with the controls.


CONCLUSIONS
Survial rates of A. formosanus can be increased by using endophytic fungi in vitro culture.
Tang
Ming-juan
MJ
Institute of Medicinal Plant, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100094, China.
Guo
Shun-xing
SX
D004156:Catechol Oxidase / Q000032:analysis; D002688:Chitinases / Q000032:analysis; D038821:Mycorrhizae / Q000502:physiology*; D029595:Orchidaceae / Q000201:enzymology / Q000254:growth & development* / Q000382:microbiology; D010650:Phenylalanine Ammonia-Lyase / Q000032:analysis; D010946:Plants, Medicinal / Q000201:enzymology / Q000254:growth & development* / Q000382:microbiology; D011509:Proteoglycans; D013559:Symbiosis; D047071:beta-Glucans / Q000032:analysis
D011509:Proteoglycans; D047071:beta-Glucans; C010770:polysaccharide-K; D004156:Catechol Oxidase; D002688:Chitinases; D010650:Phenylalanine Ammonia-Lyase