Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography.
This paper deals with an unusual application for a copolymer of styrene-1% divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. The so-called aminomethyl resin (AMR), to date only employed for peptide synthesis, swelled appreciably in water and was used successfully to purify negatively charged peptides. By correlating swelling degree of beads with pH of the media, it was possible to estimate that the AMR amino group pK(a) is approximately 5.5. In addition, the synthesized acetyl-(NANP)3-AMR succeeded in the affinity interaction with large antibody molecules related to malaria transmission and raised previously against this dodecapeptide sequence.
Carvalho
Regina S H
RS
Department of Biophysics, Universidade Federal de Sao Paulo, Rua 3 de Maio 100, CEP 04044-020, São Paulo, SP, Brazil.
Ianzer
Danielle A
DA
Malavolta
Luciana
L
Rodrigues
Mauricio M
MM
Cilli
Eduardo M
EM
Nakaie
Clovis R
CR
D000837:Anion Exchange Resins; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000138:chemical synthesis*; D011137:Polystyrenes / Q000737:chemistry*
D000837:Anion Exchange Resins; D010455:Peptides; D011137:Polystyrenes