title: [Analysis of the Ca2+ response of mycelial fungi to external effects by the recombinant aequorin method].
abstract: Using the mutant strain Aspergillus awamori 66A producing a recombinant Ca2+-dependent photosensitive protein aequorin, the dynamics of Ca2+ was studied for the first time in the cytosol of the micromycetes exposed to stressful factors, such as an increase in extracellular Ca2+ to 50 mM, hypoosmotic shock, and mechanical shock. Cell response to stress proved to involve an increase in the Ca2+ concentration in the cytosol, which was determined from the amplitude of aequorin luminescence and the time of the amplitude enhancement and relaxation. The level of Ca2+ response depended on the physiological stimulus. Inhibitory analysis with various agents that block Ca2+ channels and with agonists that specifically enhance the activity of the channels suggested that (1) the level of Ca2+ in the cytosol of micromycetes increases in response to stress because of the ion influx from both the growth medium and intracellular reservoirs and (2) the potential-dependent transport systems play the major role in the Ca2+ influx into the cytosol of the micromycete cells.
authors.forename: O V
authors.initials: OV
authors.lastname: Kozlova
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Egorov
authors.forename: F G
authors.initials: FG
authors.lastname: Kupriianova-Ashina
authors.forename: N
authors.initials: N
authors.lastname: Read
authors.forename: G I
authors.initials: GI
authors.lastname: El'-Registan
mesh_terms: D000331:Aequorin / Q000096:biosynthesis / Q000235:genetics; D001230:Aspergillus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015220:Calcium Channels / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D009997:Osmotic Pressure; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D015220:Calcium Channels; D011994:Recombinant Proteins; D000331:Aequorin; D002122:Calcium Chloride