title: Truncated midkine induces transformation of cultured cells and short latency of tumorigenesis in nude mice.
abstract: To test whether truncated midkine (tMK), an alternative spliced form of exon 3, induces the transformation of mammalian cells, tMK cDNA was transfected into SW-13 cells. Although, the growth of MK transfectant (SW-13/MK) cells was close to those of the parent SW-13 and vector transfectant (SW-13/empty) cells, the growth of tMK transfectant (SW-13/tMK) cells was significantly promoted compared with that of the above three cells. Both SW-13/tMK and SW-13/MK formed colonies in 0.35% soft agar, indicating that tMK and MK induce mammalian cell transformation. SW-13/tMK frequently formed solid tumor after its subcutaneous injection into nude mice. Additionally, in SW-13/tMK and SW-13/MK-injected mice, advanced visible tumors were detected compared with that in the case of SW-13/empty-injected mice as control. These findings indicate that tMK induces mammalian cell transformation and promotes tumor establishment in vivo.
authors.affiliation: Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Nobata
authors.forename: Takao
authors.initials: T
authors.lastname: Shinozawa
authors.forename: Akio
authors.initials: A
authors.lastname: Sakanishi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D016207:Cytokines / Q000235:genetics*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D000078792:Midkine; D009374:Neoplasms, Experimental / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D000078792:Midkine