Blood-brain barrier disruption and lesion localisation in experimental autoimmune encephalomyelitis with predominant cerebellar and brainstem involvement.
The role of the blood-brain barrier (BBB) in determining lesion distribution was assessed in an atypical model of experimental autoimmune encephalomyelitis (EAE) induced in C3H/HeJ mice by immunisation with peptide 190-209 of myelin proteolipid protein, which can result in two distinct types of EAE, each with distinct lesion distribution. Areas of the BBB showing constitutively greater permeability in naive mice did not correlate with the lesion distribution in EAE. BBB disruption occurred only in sites of inflammatory cell infiltration. Irrespective of the clinical type, the BBB was disrupted in the cerebellum and brainstem. Pertussis toxin had no effect on lesion distribution. Thus, lesion distribution is not influenced solely by BBB permeability.
Muller
Diane M
DM
Neuroimmunology Research Centre, The University of Queensland, Clinical Sciences Building, Royal Brisbane and Women's Hospital, Herston, Brisbane Q4029, Australia.
Pender
Michael P
MP
Greer
Judith M
JM
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001812:Blood-Brain Barrier / Q000276:immunology*; D001933:Brain Stem / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology*; D002199:Capillary Permeability / Q000276:immunology; D002531:Cerebellum / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D006735:Horseradish Peroxidase / Q000008:administration & dosage / Q000032:analysis; D007262:Infusions, Intravenous; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000378:metabolism; D037342:Pertussis Toxin / Q000008:administration & dosage; D013116:Spinal Cord / Q000201:enzymology / Q000473:pathology; D013194:Staining and Labeling
D015415:Biomarkers; D006735:Horseradish Peroxidase; D009195:Peroxidase; D037342:Pertussis Toxin