Physicochemical characterization and biological effects of inulin enzymatically synthesized from sucrose.
We first developed the method to produce inulin from sucrose using an enzyme from Bacillus sp. 217C-1. Synthesized inulin consists of a linear polymer having beta(2-1) linkages of d-fructose with one terminal glucose. The synthesized inulin has similar properties (pH and thermal stability, Maillard reaction, and in vitro fermentation) to plant-derived inulin. The marked difference is the polydispersity of the inulin chain length. Synthesized inulin with a narrow degree range of fructose polymerization shows better solubility in water than plant-derived inulin. Synthesized inulin (5%, w/w) treatment for 12 weeks reduced the elevation in body weight and serum and liver lipids in rats fed high fat- and high sucrose-supplemented diets, and blood glucose in rats fed a standard diet. Synthesized inulin (15%, w/w) significantly suppressed the elevation in blood glucose of human healthy subjects after dextrin loading. These results suggest that daily intake of synthesized inulin modulates carbohydrate and lipid metabolism.
Wada
Tadashi
T
Fuji Nihon Seito Corporation, 1-4-10 Shimizuseikai, Shizuoka City, Shizuoka 424-8737, Japan.
Sugatani
Junko
J
Terada
Etsuko
E
Ohguchi
Masao
M
Miwa
Masao
M
D000818:Animals; D001407:Bacillus / Q000201:enzymology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001786:Blood Glucose / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D007444:Inulin / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis / Q000097:blood; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D051381:Rats; D013395:Sucrose / Q000378:metabolism*
D001786:Blood Glucose; D008055:Lipids; D013395:Sucrose; D007444:Inulin