title: The involvement of polyamines as substrates of transglutaminase in zonal different hepatocyte proliferation after partial hepatectomy.
abstract: We have recently demonstrated the inverse correlation between transglutaminase (TGase) activity and DNA synthesis in periportal hepatocytes (PPH) and perivenous hepatocytes (PVH) at 1 d after partial hepatectomy. In order to elucidate a role of polyamines as substrates of TGase in the differential growth capacities between PPH and PVH from regenerating liver, we investigated the zonal differences in alteration of ornithine decarboxylase (ODC) activity and polyamines. In two subpopulations, the inverse correlation between DNA synthesis and epsilon-(gamma-glutamyl) lysine (Gln-Lys) cross-linking catalyzed by TGase was demonstrated at 1 d after partial hepatectomy. ODC activity in PPH significantly increased with a peak at 1 d after partial hepatectomy, whereas did not in PVH. Protein-binding SPD in PPH also transiently increased with a peak at 1 d after partial hepatectomy, but did not in PVH. These results suggest that at 1 d after partial hepatectomy, in PPH, the inhibition of Gln-Lys cross-linking by the formation of N-gamma-glutamyl SPD leads to the increase of DNA synthesis, whereas in PVH, enhanced formation of Gln-Lys cross-linking leads to the lower DNA synthesis.
authors.affiliation: Department of Radiopharmacy, Tohoku Pharmaceutical University, 4-4-1 Komatsushima. Aoba-ku, Sendai, Miyagi 981-8558, Japan. y-ohtake@tohoku-pharm.ac.jp
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ohtake
authors.forename: Satoko
authors.initials: S
authors.lastname: Suyama
authors.forename: Shinya
authors.initials: S
authors.lastname: Abe
authors.forename: Naoko
authors.initials: N
authors.lastname: Sato
authors.forename: Soichi
authors.initials: S
authors.lastname: Kojima
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukumoto
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Ohkubo
mesh_terms: D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism*; D049109:Cell Proliferation; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D015317:Biogenic Polyamines; D011503:Transglutaminases