Development of a high-resolution magic-angle spinning nuclear magnetic resonance identity assay of the capsular polysaccharide from Haemophilus influenzae type b present in cetavlon precipitate.
We describe the use of high-resolution magic-angle spinning nuclear magnetic resonance to control the identity of the capsular polysaccharide from Haemophilus influenzae type b (Hib) present in the cetavlon precipitate. This step is one of the earliest in the purification of this polysaccharide, which is further used in the production of Hib polysaccharide-protein conjugate vaccine. The effects of sample procedure and magnetic field strength have been investigated. Since this assay is rapid and simple, it may represent a useful technique for characterization of polysaccharides present in complex and insoluble matrices. Moreover, it allows a rapid evaluation of the structure of the produced polysaccharides very early on during the production process and is as such an essential analytical tool before starting the purification process.
Wieruszeski
Jean-Michel
JM
CNRS/Université de Lille 2, UMR 8525, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France.
Talaga
Philippe
P
Lippens
Guy
G
D016667:Bacterial Capsules; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D011232:Chemical Precipitation; D018073:Haemophilus Vaccines / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000302:isolation & purification
D002593:Cetrimonium Compounds; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial; D000077286:Cetrimonium