A microfabricated capillary electrophoresis chip with multiple buried optical fibers and microfocusing lens for multiwavelength detection.
We present a new microfluidic device utilizing multiwavelength detection for high-throughput capillary electrophoresis (CE). In general, different fluorescent dyes are only excited by light sources with appropriate wavelengths. When excited by an appropriate light source, a fluorescent dye emits specific fluorescence signals of a longer wavelength. This study designs and fabricates plastic micro-CE chips capable of performing multiple-wavelength fluorescence detection by means of multimode optic fiber pairs embedded downstream of the separation channel. For detection purposes, the fluorescence signals are enhanced by positioning microfocusing lens structures at the outlets of the excitation fibers and the inlets of the detection fibers, respectively. The proposed device is capable of detecting multiple samples labeled with different kinds of fluorescent dyes in the same channel in a single run. The experimental results demonstrate that various proteins, including bovine serum albumin and beta-casein, can be successfully injected and detected by coupling two light sources of different wavelengths to the two excitation optic fibers. Furthermore, the proposed device also provides the ability to measure the speed of the samples traveling in the microchannel. The developed multiwavelength micro-CE chip could have significant potential for the analysis of DNA and protein samples.
Hsiung
Suz-Kai
SK
Department of Engineering Science, National Cheng Kung University, Taiwan 701.
Lin
Che-Hsin
CH
Lee
Gwo-Bin
GB
D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005336:Fiber Optic Technology / Q000295:instrumentation; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D007909:Lenses; D046208:Microchip Analytical Procedures / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D008904:Miniaturization; D055100:Optical Fibers; D019904:Polymethyl Methacrylate; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D012235:Rhodamines; D019904:Polymethyl Methacrylate; D016650:Fluorescein-5-isothiocyanate; C029773:rhodamine B