[Apoptotic, autophagic, and oncotic death of cardiomyocytes in anoxia-reoxygenation].
In experiments on isolated rat neonatal cardiomyocytes under anoxia-reoxygenation it was established the different types of cell death: non-programmed (oncotic) and programmed (apoptotic and autophagic). Propidium iodide and Hoechst 33342 staining revealed that alive, necrotic and apoptotic cell ratio after anoxia-reoxygenation was 77%, 14%, 9% respectively (86%, 9.6% and 4.4% in control). Electron microscopy of neonatal rat cardiomyocytes allows us to clarify the ultrastructural peculiarities of each type of cell death. Cytoplasm hydratation, swelling, cell membrane components vacuolization and sarcollemmal integrity alteration were typical for necrosis. The features of apoptosis include: cytoplasm osmiophylia, cell organelle destruction, chromatin condensation and nucleus fragmentation as well as blebs and apoptotic body formation. Autophagic cell death was characterized by the presence of large amount of different size vacuoles accompanied by significant cytoplasm osmiophylia, chromatin condensation or nucleus picnosis (nucleus fragmentation was not observed in these cells) and preservation of sarcollemmal integrity. The autophagic cardiomyocytes destruction was proved by specific monodansylcadaverine staining of vacuolar structures. We first showed the anoxia-reoxygenation-related autophagic cell death of neonatal rat cardiomyocytes. These results strongly suggest the necessity of autophagic cell death investigation in pathogenesis of cardiovascular diseases.
Tumanovs'ka
L V
LV
Dosenko
V Ie
VIe
Nahibin
V S
VS
Makohon
N V
NV
Moĭbenko
O O
OO
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D008854:Microscopy, Electron; D032383:Myocytes, Cardiac / Q000648:ultrastructure*; D009336:Necrosis; D010100:Oxygen / Q000502:physiology*; D051381:Rats
D010100:Oxygen