title: Dynamic complex formation during the yeast cell cycle.
abstract: To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.
authors.affiliation: Center for Biological Sequence Analysis, Technical University of Denmark, DK-2800 Lyngby, Denmark.
authors.forename: Ulrik
authors.initials: U
authors.lastname: de Lichtenberg
authors.forename: Lars Juhl
authors.initials: LJ
authors.lastname: Jensen
authors.forename: Søren
authors.initials: S
authors.lastname: Brunak
authors.forename: Peer
authors.initials: P
authors.lastname: Bork
mesh_terms: D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008938:Mitosis; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D021122:Protein Subunits / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D016213:Cyclins; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D017489:CDC28 Protein Kinase, S cerevisiae