Diversity within the Campylobacter jejuni type I restriction-modification loci.
The type I restriction-modification (hsd) systems of 73 Campylobacter jejuni strains were characterized according to their DNA and amino acid sequences, and/or gene organization. A number of new genes were identified which are not present in the sequenced strain NCTC 11168. The closely related organism Helicobacter pylori has three type I systems; however, no evidence was found that C. jejuni strains contain multiple type I systems, although hsd loci are present in at least two different chromosomal locations. Also, unlike H. pylori, intervening ORFs are present, in some strains, between hsdR and hsdS and between hsdS and hsdM. No definitive function can be ascribed to these ORFs, designated here as rloA-H (R-linked ORF) and mloA-B (M-linked ORF). Based on parsimony analysis of amino acid sequences to assess character relatedness, the C. jejuni type I R-M systems are assigned to one of three families: 'IAB', 'IC' or 'IF'. This study confirms that HsdM proteins within a family are highly conserved but share little homology with HsdM proteins from other families. The 'IC' hsd loci are >99 % identical at the nucleotide level, as are the 'IF' hsd loci. Additionally, whereas the nucleotide sequences of the 'IAB' hsdR and hsdM genes show a high degree of similarity, the nucleotide sequences of the 'IAB' hsdS and rlo genes vary considerably. This diversity suggests that recombination between 'IAB' hsd loci would lead not only to new hsdS alleles but also to the exchange of rlo genes; five C. jejuni hsd loci are presumably the result of such recombination. The importance of these findings with regard to the evolution of C. jejuni type I R-M systems is discussed.
Miller
William G
WG
Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA.
Pearson
Bruce M
BM
BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich, UK.
Wells
Jerry M
JM
University of Amsterdam, Swammerdam Institute for Life Sciences, Amsterdam, The Netherlands.
Parker
Craig T
CT
Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA.
Kapitonov
Vladimir V
VV
Genetic Information Research Institute, Mountain View, CA 94043, USA.
Mandrell
Robert E
RE
Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA.
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D016123:Campylobacter jejuni / Q000201:enzymology* / Q000235:genetics; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics; D015253:Deoxyribonucleases, Type I Site-Specific / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; D029968:Escherichia coli Proteins; C113046:HSDS protein, Bacteria; C109829:HsdM protein, Bacteria; D015253:Deoxyribonucleases, Type I Site-Specific; C109830:HsdR protein, E coli