Prolactin affects both survival and differentiation of T-cell progenitors.
We have analysed the in vitro effects of prolactin on thymocyte development concluding that PRL favours the survival and differentiation of T-cell progenitors. Fetal, adult thymocytes and CD45(+) fetal liver lymphoid progenitors express PRL-R. PRL induces survival, proliferation and differentiation of lymphoid progenitors whereas both an anti-PRL antiserum and an anti-PRL-R mAb block T-cell development accumulating CD25(+)DN (CD4(-)CD8(-)) cells. Furthermore, IL2 rescues the blockade of T-cell development in FTOC treated with anti-PRL antiserum but PRL does not recover cultures treated with an anti-IL2R alpha chain mAb, which drastically blocks the T-cell development. These results support IL2/IL2R mediation of PRL effects on developing thymocytes.
Carreño
Paz C
PC
Department of Cell Biology, Faculty of Biology, Complutense University, 28040 Madrid, Spain.
Sacedón
Rosa
R
Jiménez
Eva
E
Vicente
Angeles
A
Zapata
Agustín G
AG
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002469:Cell Separation; D002470:Cell Survival / Q000235:genetics / Q000276:immunology; D047109:Fetal Development / Q000235:genetics / Q000276:immunology / Q000502:physiology; D007155:Immunologic Factors / Q000502:physiology*; D007376:Interleukin-2 / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000502:physiology; D038041:Lymphopoiesis / Q000276:immunology / Q000502:physiology*; D051379:Mice; D009924:Organ Culture Techniques; D011388:Prolactin / Q000276:immunology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015375:Receptors, Interleukin-2 / Q000502:physiology; D011981:Receptors, Prolactin / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000276:immunology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
D007155:Immunologic Factors; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011981:Receptors, Prolactin; D011388:Prolactin