Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails.
It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes.
Kunitomo
Hirofumi
H
Molecular Genetics Research Laboratory, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. kuni@gen.s.u-tokyo.ac.jp
Uesugi
Hiroko
H
Kohara
Yuji
Y
Iino
Yuichi
Y
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D002923:Cilia; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D015801:Helminth Proteins / Q000235:genetics; D047468:Immunoprecipitation / Q000379:methods*; D009475:Neurons, Afferent / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics / Q000276:immunology; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis
D015801:Helminth Proteins; D009842:Oligopeptides; D010455:Peptides; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C400683:che-2 protein, C elegans; C085706:FLAG peptide