title: Comparison of trypsin treatment method and standard laboratory technique for diagnosis of dermatomycosis.
abstract: Dermatomycosis is prevalent worldwide. Discrepancy between microscopic examination and culture findings can create problems in the diagnosis of this common infection. In this study, samples from 60 patients were processed after trypsin treatment and examined by neutral red staining to distinguish viable and non-viable fungal elements. The trypsin treatment method was compared with standard laboratory techniques. A higher number of direct-microscopy-positive, culture-negative samples were obtained without trypsin treatment. Trypsin treatment increased the isolation of fungi from clinical samples, and neutral red staining was able to distinguish viable fungal elements.
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India. I-xess@yahoo.com
authors.forename: Immaculata
authors.initials: I
authors.lastname: Xess
authors.forename: Purva
authors.initials: P
authors.lastname: Mathur
authors.forename: Chandra Shekar
authors.initials: CS
authors.lastname: Sirka
authors.forename: Uma
authors.initials: U
authors.lastname: Banerjee
mesh_terms: D002175:Candida / Q000302:isolation & purification; D004396:Coloring Agents; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology; D004821:Epidermophyton / Q000302:isolation & purification; D006801:Humans; D008853:Microscopy / Q000379:methods; D009499:Neutral Red; D014005:Tinea / Q000175:diagnosis / Q000382:microbiology; D046509:Tissue Culture Techniques; D014249:Trichophyton / Q000302:isolation & purification; D014357:Trypsin
chemical_list: D004396:Coloring Agents; D009499:Neutral Red; D014357:Trypsin