title: Molecular cloning of leukocyte cell-derived chemotaxin 2 in rainbow trout.
abstract: In humans, leukocyte cell-derived chemotaxin 2 (LECT2) is a 16kDa chemotactic protein that consists of 133 amino acids and three intramolecular disulphide bonds. Although it was originally demonstrated to have a chemotactic function in vitro, recent data sustain a further multifunctional role of LECT2 that extends from cell growth, differentiation, damage/repair process and carcinogenesis to autoimmune diseases. The in vivo function of LECT2 protein still remains obscure. In order to study the phylogeny of LECT2, a full-length cDNA clone of LECT2 gene, 720 bp in size, was isolated in rainbow trout (Oncorhynchus mykiss). Its deduced amino acid sequence of 156 residues, presents 40, 45 and 61% overall identity to human, mouse and carp LECT2 proteins, respectively. In contrast to mammalian LECT2 protein, trout LECT2 protein reveals two potential N-glycosylation sites. Phylogenetic analysis shows that trout LECT2 is clustered with the known homologous proteins. Trout LECT2 mRNA is predominately expressed in liver and spleen, showing lower expression in kidney, intestine, heart and brain.
authors.affiliation: Department of Biology, School of Medicine, University of Patras, Rion 26500 Patra, Greece.
authors.forename: Petros A
authors.initials: PA
authors.lastname: Kokkinos
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kazantzi
authors.forename: Georgia
authors.initials: G
authors.lastname: Sfyroera
authors.forename: Ioannis K
authors.initials: IK
authors.lastname: Zarkadis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002630:Chemotactic Factors / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D017931:DNA Primers; D015723:Gene Library; D007962:Leukocytes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013154:Spleen / Q000378:metabolism
chemical_list: D002630:Chemotactic Factors; D017931:DNA Primers