[Infrared spectrum and nuclear magnetic resonance analysis of the alkaloid extract of Gelsemium produced in east Guangdong province].
OBJECTIVE
To identify the structure of the alkaloid extract of Gelsemium from east Guangdong province of China.


METHODS
Fourier transform infrared (FTIR) absorption spectrometry, hydrogen and carbon spectra of nuclear magnetic resonance (NMR) and distortionless enhancement by polarization transfer (DEPT) analysis were used for the structural identification of the alkaloid exstract of Gelsemium.


RESULTS
The frequency, intensity and shape of the extract's characteristic peaks in infrared absorption spectra (4,000.0-400.0 cm(-1)) and (1)H NMR, (13)C NMR were recognized and compared. The molecular structure of the sample was consistent with the theoretically derived model.


CONCLUSIONS
The extract is structurally identical to koumine, which may provide evidence for its safe clinical application and establishment of Chinese medicine fingerprint database of Gelsemium.
Wang
Zhi-rui
ZR
Department of Pharmaceutics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. wangzr@fimmu.com
Zhang
Lan-lan
LL
Huang
Chang-quan
CQ
Zhang
Zhong-yi
ZY
Lin
Jing-ming
JM
D000470:Alkaloids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D002681:China; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031450:Gelsemium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013055:Spectrophotometry, Infrared
D000470:Alkaloids; D004365:Drugs, Chinese Herbal