title: Development and validation of a liquid chromatography and tandem mass spectrometry method for determination of roscovitine in plasma and urine samples utilizing on-line sample preparation.
abstract: Roscovitine, a purine analogue that selectively inhibits cyclin-dependent kinases, has been considered as a potential anti-tumor drug. The determination of roscovitine in plasma and urine was performed using microextraction in packed syringe as on-line sample preparation method with liquid chromatography and tandem mass spectrometry. The sampling sorbent utilized was polystyrene polymer. 2H3-lidocaine was used as internal standard. The limit of detection for roscovitine was as low as 0.5 ng/mL and the lower limit of quantification was 1.0 ng/mL. The accuracy and precision values of quality control samples were between +/-15% and < or =11%, respectively. The calibration curve was obtained within the concentration range 0.5-2000 ng/mL in both plasma and urine. The regression correlation coefficients for plasma and urine samples were > or =0.999 for all runs. The present method is miniaturized and fully automated and can be used for pharmacokinetic and pharmacodynamic studies.
authors.affiliation: Department of Medicine, Division of Hematology, Laboratory of Hematology, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden.
authors.forename: Marina
authors.initials: M
authors.lastname: Vita
authors.forename: Patrik
authors.initials: P
authors.lastname: Skansen
authors.forename: Moustapha
authors.initials: M
authors.lastname: Hassan
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011687:Purines / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D000077546:Roscovitine; D012680:Sensitivity and Specificity
chemical_list: D004791:Enzyme Inhibitors; D011687:Purines; D000077546:Roscovitine