title: In vivo expression of the 25-kDa laminin-binding protein of Helicobacter pylori.
abstract: The gastroduodenal pathogen Helicobacter pylori has been shown to inhibit the interaction between the extracellular matrix protein laminin and its receptor on gastric epithelial cells, potentially contributing to a loss of mucosal integrity. As a 25-kDa outer membrane protein of H. pylori in association with the bacterial lipopolysaccharides (LPS) mediates attachment to laminin, the aim of this study was to determine whether the 25-kDa protein is produced by H. pylori in infected hosts. We examined the immune response to the 25-kDa laminin binding protein in 12 paediatric patients; samples from a H. pylori-negative healthy adult were used as controls. In immunoblotting, antibodies to a 25-kDa protein were found in the serum and saliva of H. pylori-positive individuals only, and using the positive sera and saliva, laminin binding to the 25-kDa protein was inhibited. Thus, the 25-kDa laminin-binding protein is produced by H. pylori in infected hosts.
authors.affiliation: Laboratory of Molecular Biochemistry, Department of Microbiology, National University of Ireland, Galway, University Road, Galway, Ireland. anthony.moran@nuigalway.ie
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Moran
authors.forename: Samantha A
authors.initials: SA
authors.lastname: Broaders
authors.forename: Anna
authors.initials: A
authors.lastname: Rapa
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Oderda
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D012463:Saliva / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D007797:Laminin