title: Differentiation of endothelial cells from human umbilical cord blood AC133-CD14+ cells.
abstract: Endothelial progenitor cells (EPCs) participate in neovascularization and are consistent with postnatal vasculogenesis. In vitro, they differentiate into endothelial cells (ECs). Prior reports have suggested that circulating human AC133(+) cells have the capacity to differentiate into ECs as progenitor cells. However, recent studies have demonstrated that circulating CD34(-)CD14(+) cells also have EPC-like properties in vitro and in vivo. We tested whether AC133(-)CD14(+) cells from human umbilical cord blood (HUCB) have the potential to differentiate into ECs. The AC133(-)CD14(+) cells were isolated from HUCB by magnetic bead selection and cultured on fibronectin-coated six-well trays in M199 medium supplemented with fetal bovine serum (FBS), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and insulin growth factor (IGF-1). The AC133(-)CD14(+) cells adhered slightly within 1 day of culture and subsequently underwent a distinct process of morphological transformation to spindle-shaped cells that sprouted from the edge of the cell clusters. After 14 days, the cells formed cord- and tubular-like structures. The AC133(-)CD14(+) cells showed a strong increase in the endothelial marker P1H12 over time, whereas CD14 decreased, and CD45 did not change, respectively. In addition, the cells expressed endothelial markers von Willebrand's factor (vWF), platelet/endothelial cell adhesion molecule-1 (PECAM-1), vascular endothelial growth factor receptor-1 (VEGFR-1)/Flt-1, VEGFR-2/Flk-1, eNOS, and VE-cadherin, but did not express Tie-2 after 7 days of culture. The present data indicate that AC133(-)CD14(+) cells from HUCB are able to develop endothelial phenotype with expression of endothelial-specific surface markers and even form cord- and tubular-like structures in vitro as progenitor cells.
authors.affiliation: Laboratory of Medical Genetics, Samsung Cheil Hospital and Women's Healthcare Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
authors.forename: Shin-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Park
authors.forename: Jin Mi
authors.initials: JM
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Moon Young
authors.initials: MY
authors.lastname: Kim
authors.forename: Jae Hyug
authors.initials: JH
authors.lastname: Yang
authors.forename: Joo Oh
authors.initials: JO
authors.lastname: Kim
authors.forename: Kyu-Hong
authors.initials: KH
authors.lastname: Choi
authors.forename: Seung Bo
authors.initials: SB
authors.lastname: Kim
authors.forename: Hyun-Mee
authors.initials: HM
authors.lastname: Ryu
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000502:physiology; D010455:Peptides / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D000943:Antigens, Differentiation; D006023:Glycoproteins; D018950:Lipopolysaccharide Receptors; C000606023:PROM1 protein, human; D010455:Peptides