title: Osteogenic effects of bioactive glass on bone marrow stromal cells.
abstract: Bioactive glass (BG) is an effective synthetic bone graft material. BG granules of narrow size range (300-355 mum) have the ability to form new bone tissue inside excavations produced by in vivo resorption. Previously, we demonstrated that BG stimulates the differentiation of cultured osteoblast precursors if the glass surface was biomimetically modified by the formation of bone-like apatite and adsorption of serum proteins. We now report that modified BG can also increase the rate at which multipotential rat bone marrow stromal cells (rMSC) will undergo osteogenesis. BG promoted rMSC osteogenesis both when cells were plated in contact with BG and when cells were not directly in contact with the BG. Alkaline phosphatase activity, a marker of bone cell differentiation, was used as an indicator for osteogenesis. Alkaline phosphatase activity of rMSCs exposed to osteoinducers such as ascorbate, dexamethasone, and BMP-2 was enhanced in the presence of BG. The stimulatory effect of BG was more pronounced in rMSC cultures with low basal alkaline phosphatase activity than in those with higher activity. The enhanced differentiation of rMSCs was associated with both a change in rMSC morphology and altered chemical composition of the cell culture media. rMSCs cultured on BG in the presence of BMP or dexamethasone exhibited a more rounded osteoblast-like appearance as compared with cells grown on tissue culture plastic. In the presence of BG, elevated levels of calcium and silicon in the culture medium were observed throughout the 7-day culture period, suggesting a continuous dissolution of surface-modified BG and resulting release of BG dissolution products. The data suggest that both surface- and solution-mediated events play a role in the osteogenic effect of BG.
authors.affiliation: Center for Bioactive Materials and Tissue Engineering, Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. radin@seas.upenn.edu
authors.forename: S
authors.initials: S
authors.lastname: Radin
authors.forename: G
authors.initials: G
authors.lastname: Reilly
authors.forename: G
authors.initials: G
authors.lastname: Bhargave
authors.forename: P S
authors.initials: PS
authors.lastname: Leboy
authors.forename: P
authors.initials: P
authors.lastname: Ducheyne
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D002478:Cells, Cultured; D002516:Ceramics; D003470:Culture Media / Q000737:chemistry; D005260:Female; D005898:Glass / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D013379:Substrate Specificity
chemical_list: D003470:Culture Media; C425330:bioactive glass 45S5; D000469:Alkaline Phosphatase