Identification by HPLC-PAD-MS and quantification by HPLC-PAD of phenylethanoid glycosides of five Phlomis species.
The qualitative and quantitative determination of phenylethanoid glycosides in methanolic extracts of five species of the genus Phlomis (Lamiaceae) has been investigated using a new reversed-phase HPLC method combined with photodiode-array detection and electrospray/MS analysis. Forsythoside B, verbascoside, samioside, alyssonoside, isoverbascoside, leucosceptosides A and B and martynoside were detected. The quantification of the above constituents was performed using echinacoside as internal standard. Knowledge of the content of phenylethanoid glycosides contributes to the chemotaxonomy of the genus.
Kirmizibekmez
Hasan
H
Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University TR-06100 Ankara, Turkey. hasankbekmez@yahoo.com
Montoro
Paola
P
Piacente
Sonia
S
Pizza
Cosimo
C
Dönmez
Ali
A
Caliş
Ihsan
I
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000737:chemistry*; D015394:Molecular Structure; D031345:Phlomis / Q000737:chemistry*; D010777:Photochemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006027:Glycosides