Ethylene and flower longevity in Alstroemeria: relationship between tepal senescence, abscission and ethylene biosynthesis.
Senescence of floral organs is broadly divided into two groups: those that exhibit sensitivity to exogenous ethylene and those that do not. Endogenous ethylene production from the former group is via a well-characterized biochemical pathway and is either due to developmental or pollination-induced senescence. Many flowers from the order Liliales are characterized as ethylene-insensitive since they do not appear to produce endogenous ethylene, or respond to exogenous ethylene treatments, however, the majority of cases studied are wilting flowers, rather than those where life is terminated by perianth abscission. The role of ethylene in the senescence and abscission of Alstroemeria peruviana cv. Rebecca and cv. Samora tepals was previously unclear, with silver treatments recommended for delaying leaf rather than flower senescence. In the present paper the effects of exogenous ethylene, 2-chloroethylphosphonic acid (CEPA) and silver thiosulphate (STS) treatments on tepal senescence and abscission have been investigated. Results indicate that sensitivity to ethylene develops several days after flower opening such that STS only has a limited ability to delay tepal abscission. Detachment force measurements indicate that cell separation events are initiated after anthesis. Endogenous ethylene production was measured using laser photoacoustics and showed that Alstroemeria senesce independently of ethylene production, but that an extremely small amount of ethylene (0.15 nl flower(-1) h(-1)) is produced immediately prior to abscission. Investigation of the expression of genes involved in ethylene biosysnthesis by semi-quantitative RT-PCR indicated that transcriptional regulation is likely to be at the level of ACC oxidase, and that the timing of ACC oxidase gene expression is coincident with development of sensitivity to exogenous ethylene.
Wagstaff
Carol
C
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK.
Chanasut
Usawadee
U
Harren
Frans J M
FJ
Laarhoven
Luc-Jan
LJ
Thomas
Brian
B
Rogers
Hilary J
HJ
Stead
Anthony D
AD
D031402:Alstroemeria / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis*; D035264:Flowers / Q000201:enzymology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008190:Lyases / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000096:biosynthesis / Q000502:physiology; D013885:Thiosulfates / Q000494:pharmacology; D013997:Time Factors
D005030:Ethylenes; D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; D013885:Thiosulfates; C095213:silver thiosulfate; C036216:ethylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase; C005073:ethephon