title: [Study on subcritical water extraction of baicalin from Radix Scutellariae coupled to high performance liquid chromatographic analysis].
abstract: A method for the extraction of baicalin from Radix Scutellariae with subcritical water coupled to high performance liquid chromatographic analysis was established. The effects of various parameters, which included the pressure, temperature, extraction time, and the particle size of the plant material as well as the solvent/sample ratio on the yield were investigated. Compared to the conventional organic solvent extraction method, the subcritical water extraction method showed shorter handling time and lower solvent consumption without waste producing. The optimal conditions of extraction of baicalin from Radix Scutellariae with subcritical water were 0.15 - 0.18 mm particle size, solvent/sample ratio of 0.2 mL/mg, 5 MPa, 130 degrees C for about 10 min. This technique seems very promising for the extraction of liposoluble substances from plant materials.
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, China. xzh1966@163.com
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Xu
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Qian
authors.forename: Sankang
authors.initials: S
authors.lastname: Liu
authors.forename: Zhangwan
authors.initials: Z
authors.lastname: Li
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis*; D031364:Scutellaria baicalensis / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C038044:baicalin