Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays.
Gene expression profiles were collected from Escherichia coli strains (OST3410, TK33, and TK31) before and after exposure to organic solvents, and the six genes that showed higher gene expression were selected. Among these genes, glpC encoding the anaerobic glycerol-3-phosphate dehydrogenase subunit C remarkably increased the organic solvent tolerance.
Shimizu
Kazunori
K
Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Hayashi
Shuhei
S
Kako
Takeshi
T
Suzuki
Maiko
M
Tsukagoshi
Norihiko
N
Doukyu
Noriyuki
N
Kobayashi
Takeshi
T
Honda
Hiroyuki
H
D003510:Cyclohexanes / Q000494:pharmacology; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012997:Solvents / Q000494:pharmacology*; D014992:Xylenes / Q000494:pharmacology
D003510:Cyclohexanes; D029968:Escherichia coli Proteins; D012997:Solvents; D014992:Xylenes; D005993:Glycerolphosphate Dehydrogenase