title: Use of the placental perfusion model to evaluate transplacental passage of Trypanosoma cruzi.
abstract: OBJECTIVE
  To determine whether Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model.
  
  
  METHODS
  A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal circulation of a placental perfusion model. Maternal and fetal perfusates, and placental tissue, were analyzed for parasitic DNA using polymerase chain reaction; perfused specimens were also examined histologically.
  
  
  RESULTS
  Intracytoplasmic amastigotes were identified in trophoblast of the incubated cell line by 24-48 hours. Following placental perfusion, T cruzi DNA was identified in all postinoculation maternal perfusate samples and postinoculation placental tissue specimens; preinoculation controls were negative.
  
  
  CONCLUSIONS
  This is the first description of the use of the human placental perfusion model to study congenital Chagas' disease, including the presence and time course of early parasitic invasion of the placenta.
authors.affiliation: Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
authors.forename: Stuart H
authors.initials: SH
authors.lastname: Shippey
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Zahn
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Cisar
authors.forename: T John
authors.initials: TJ
authors.lastname: Wu
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Satin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D014355:Chagas Disease / Q000151:congenital / Q000635:transmission*; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D010477:Perfusion; D010920:Placenta / Q000469:parasitology*; D011247:Pregnancy; D014327:Trophoblasts / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan