Nucleation and growth in one dimension. II. Application to DNA replication kinetics.
Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I (t) and growth speed v from data. We discuss generic experimental problems: namely, spatial inhomogeneity, measurement noise, and finite-size effects. After evaluating how each of these affects the measurements of I (t) and v , we give guidelines for the design of experiments. These ideas are then discussed in the context of the DNA-replication experiments.
Jun
Suckjoon
S
Department of Physics, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6. s.jun@amolf.nl
Bechhoefer
John
J
D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004261:DNA Replication; D019281:Dimerization; D007700:Kinetics; D046911:Macromolecular Substances / Q000737:chemistry; D008956:Models, Chemical; D015233:Models, Statistical
D046911:Macromolecular Substances; D004247:DNA