Introduction of tyramide signal amplification (TSA) to pre-embedding nanogold-silver staining at the electron microscopic level.
The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.
Lee
Seung-won
SW
Department of Anatomy, Chonnam National University Medical School, Gwangju, Korea. seunglee@chonnam.ac.kr
Lee
Song Eun
SE
Ko
Seong Hyuk
SH
Hong
Eun Kyoung
EK
Nam
Kwang Il
KI
Nakamura
Kei-ichiro
K
Imayama
Shuhei
S
Park
Yeong-Joon
YJ
Ahn
Kyu Youn
KY
Bae
Choon Sang
CS
Kim
Baik Yoon
BY
Park
Sung Sik
SS
D001324:Autoantigens; D045744:Cell Line, Tumor; D006046:Gold; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008545:Melanoma; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D016622:Silver Staining; D014439:Tyramine
D001324:Autoantigens; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; D006046:Gold; D014439:Tyramine