The use of turbulent flow chromatography and the isocratic focusing effect to achieve on-line cleanup and concentration of neat biological samples for low-level metabolite analysis.
The use of turbulent flow chromatography in conjunction with column switching isocratic focusing was used to perform on-line sample cleanup and concentration of neat rat plasma for the identification of low-level metabolites. The concentration was achieved by focusing multiple injections, which were cleaned by a turbulent flow column, onto an analytical column prior to elution into the mass spectrometer. In addition, the first application of turbulent flow chromatography for on-line sample cleanup of neat bile samples is reported. The on-line cleanup and concentration method extracts and concentrates a sample 20-fold in 1 h, and is completely automated.
Herman
J L
JL
Cephalon, Inc., West Chester, PA 19380-4245, USA. jherman@cephalon.com
D000717:Anatomy, Comparative; D000818:Animals; D001646:Bile / Q000737:chemistry*; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D008832:Microchemistry / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001798:Blood Proteins