High level expression of recombinant chicken interferon-alpha using baculovirus.
Bioactive recombinant chicken interferon-alpha (ChIFN-alpha) was expressed in a baculovirus system. For easy purification, it was expressed as ChIFN-alpha bearing histidine hexamer (His-tag) at C-terminal, designated ChIFN-alphaHis. The expressed proteins were detected by SDS-PAGE analysis with Coomassie brilliant blue staining as around 23 and 19 kDa bands thought to be immature and matured ChIFN-alphaHis respectively. The purified ChIFN-alphaHis with a nickel chelated column showed anti-viral activity in vitro.
Ruttanapumma
Ruttapong
R
Laboratory of Poultry Diseases, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Nakamura
Masayuki
M
Takehara
Kazuaki
K
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D016367:Baculoviridae / Q000378:metabolism*; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression; D005822:Genetic Vectors; D007370:Interferon Type I / Q000096:biosynthesis* / Q000494:pharmacology; D011994:Recombinant Proteins; D018411:Spodoptera
D000998:Antiviral Agents; D007370:Interferon Type I; D011994:Recombinant Proteins