title: [Study on post-translational processing and active forms of the novel metastasis-associated protein SNC19].
abstract: To investigate the relationship between SNC19 protein and cancer metastasis.
  
  
  
  Expression of SNC19 protein in cancer cell lines and tissues was examined by Western blot analysis using anti-SNC19 monoclonal antibody. In addition, Psectag2A-SNC19(ORF) eukaryotic expression vector was constructed and transfected into BCAP37 cells. After the target protein was expressed and purified, processing forms of SNC19 protein were further identified using anti-His mAb and each form was assayed for its gelatinase activity.
  
  
  
  Different expression and post-translational processing of the SNC19 proteins in the cancer cell lines and intestinal tissues were detected.BCAP37 cells transfected full-length SNC19 (ORF) generated two different sized proteins in cell lysates, 120 and 75 kD; 75 kD was detected to have proteolytic activity by gelatin zymography.
  
  
  
  SNC19 protein presents different expression and post-translational processing in the cancer cells and tissues, of which 75 kD was identified to have gelatinase activity.
authors.affiliation: The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.forename: Xiao-Ye
authors.initials: XY
authors.lastname: Hu
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Sun
authors.forename: Su-zhan
authors.initials: SZ
authors.lastname: Zhang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000091402:Prohibitins; D011499:Protein Processing, Post-Translational; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; C000717331:PHB2 protein, human; D000091402:Prohibitins; D012697:Serine Endopeptidases; D018093:Gelatinases