[Developments in preparation and experimental method of solid phase microextraction fibers].
Solid phase microextraction (SPME) is a simple and effective adsorption and desorption technique, which concentrates volatile or nonvolatile compounds from liquid samples or headspace of samples. SPME is compatible with analyte separation and detection by gas chromatography, high performance liquid chromatography, and other instrumental methods. It can provide many advantages, such as wide linear scale, low solvent and sample consumption, short analytical times, low detection limits, simple apparatus, and so on. The theory of SPME is introduced, which includes equilibrium theory and non-equilibrium theory. The novel development of fiber preparation methods and relative experimental techniques are discussed. In addition to commercial fiber preparation, different newly developed fabrication techniques, such as sol-gel, electronic deposition, carbon-base adsorption, high-temperature epoxy immobilization, are presented. Effects of extraction modes, selection of fiber coating, optimization of operating conditions, method sensitivity and precision, and systematical automation, are taken into considerations in the analytical process of SPME. A simple perspective of SPME is proposed at last.
Yi
Xu
X
Chem & Chem Engineering College, Chongqing University, Chongqing 400044, China. xuyicd@cta.cq.cn
Fu
Yujie
Y
D002849:Chromatography, Gas / Q000379:methods* / Q000639:trends*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity