title: Electron-optical characterization of nano- and micro-particles in raw and treated waters: an overview.
abstract: State-of-the-art information is presented on the analysis, by transmission electron microscopy (TEM), of aquatic colloidal particles in the size range of 3 to 500 nm least dimension, with a focus on nanoparticles (1-100 nm). Case studies include selections from both natural waters and waters undergoing treatment. The "species" of nano-particles receiving the greatest attention are: humic substances, polysaccharide fibrils, hydrous iron oxides, viruses, clay minerals, refractory cell debris, and heavy metal agglomerates on biological surfaces. Artifacts and how to both detect and minimize them are outlined. Correlative use of TEM with other imaging techniques is emphasized, along with associated spectroscopy. Noted is the potential of computerized image analysis for quantifying colloids on a "per colloid species" basis, using water samples centrifuged onto electron microscope grids.
authors.affiliation: Aquatic Ecosystem Management Research Branch, National Water Research Institute, Burlington, Ontario, Canada L7R 4A6. Gary.Leppard@ec.gc.ca
authors.forename: G G
authors.initials: GG
authors.lastname: Leppard
authors.forename: D
authors.initials: D
authors.lastname: Mavrocordatos
authors.forename: D
authors.initials: D
authors.lastname: Perret
mesh_terms: D003102:Colloids / Q000737:chemistry; D008854:Microscopy, Electron / Q000379:methods*; D036103:Nanotechnology; D010316:Particle Size; D014867:Water / Q000032:analysis / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D003102:Colloids; D014867:Water