[Determination of verapamil in dog serum and tissues by reversed-phase high performance liquid chromatography].
A method for determination of verapamil (VRPM) concentrations in dog serum and tissues was established. The study was carried out on a high performance liquid chromatograph with a reversed-phase C18 column, a mobile phase of acetate-methanol-triethylamine (40:60:1, v/v) at 1.0 mL/min, and UV detection at 228 nm. Diazepam was used as the internal standard. The detection limits of VRPM concentrations in serum and tissues were determined to be 50 microg/L and 50 ng/g, respectively. Good linearities were observed for VRPM in serum and in liver tissue (r > 0.999). The precision of the method was good and the recoveries of VRPM were higher than 90.80%. This method is rapid, accurate and convenient for determination of VRPM concentrations in serum and tissues.
Sun
Xin
X
Experimentation Center, First Affiliated Hospital, Anhui Medical University, Hefei 230022, China. liuliuwn@mail.hf.ah.cn
Yin
Qingsheng
Q
Chen
Dianliang
D
Dong
Xuejun
X
Zhou
Lichun
L
Zhang
Hui
H
Fan
Pingsheng
P
D000818:Animals; D002121:Calcium Channel Blockers / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004285:Dogs; D007202:Indicators and Reagents; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D014700:Verapamil / Q000032:analysis* / Q000097:blood
D002121:Calcium Channel Blockers; D007202:Indicators and Reagents; D014700:Verapamil