[The effect of nandrolone phenylpropionate on fibroblasts after injury in rats].
OBJECTIVE
To explore the effect of nandrolone phenylpropionate (NP) on rats' fibroblasts after injury.


METHODS
After being isolated from granulation tissue of a one-month Wistar rat,the fibroblasts(FB) were grouped into 5 groups and cultured in RPMI1640 and 5% FBS culturing liquid with 0.5, 1.0, 5.0, 10.0, and 15.0 microg/ml NP respectively. The control group was cultured in RPMI1640 and 5% FBS culturing liquid Fibroblasts were isolated from granulation tissue of a one-month Wistar rat and cultured with RPMI1640 culturing liquid with 5% FBS added different doses of NP from 0.5 approximately 15.0 microg/ml in NP group, but only 5% FBS for the control group. Cell validity of fibroblasts was measured by MTT. The proliferative index (PI) of the most effected group was measured by flow cytometry.


RESULTS
Compared with control group, higher FB validity occurred in every NP group (P < 0.05) The PI of FB in every NP group measured by flow cytometry was significantly higher than that in control group (P < 0.01).


CONCLUSIONS
NP can promote the replication and proliferation of FB.
Liu
Ning
N
Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu Sichuan 610041, P R China.
Ying
Cen
C
Li
Kai
K
Liu
Xiaoxue
X
D045930:Anabolic Agents / Q000494:pharmacology; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects
D045930:Anabolic Agents; D009277:Nandrolone; C008580:nandrolone phenpropionate