title: A cytosine-rich region upstream of start codons serving as a signal for initiation of translation in Encephalitozoon cuniculi?
abstract: Encephalitozoon cuniculi, an intracellular eukaryote frequently infecting immunodeficient humans, displays pronounced compaction in its genes. Short-leaded mRNA has been observed which has led to speculation into alternative mechanisms of translation initiation. It has been proposed that a 'downstream box' could serve as an initiation signal. In this report, non-randomness analysis was used to study the genes of E. cuniculi. Surprisingly, it was found that the region 5-10nt upstream of start codons is highly cytosine-enriched and this phenomenon clearly differentiates genes of predicted high and low expressivity. These two groups of genes can, on the other hand, not be clearly differentiated from non-randomness plots downstream of their start codons. The data thus do not support the 'downstream box' hypothesis but seem to suggest that initiation is depending on the region immediately upstream of start codons. The cytosine richness has no known parallel in other eukaryotes, prokaryotes or archaebacteria.
authors.affiliation: Institute of Pharmacology, Danish University of Pharmaceutical Sciences, DK-2100 Copenhagen Ø, Denmark. anfu@dfuni.dk
authors.forename: Anders
authors.initials: A
authors.lastname: Fuglsang
mesh_terms: D000818:Animals; D001482:Base Composition; D018387:Codon, Initiator; D003596:Cytosine; D009670:Encephalitozoon cuniculi / Q000235:genetics*; D017125:Genes, Protozoan; D014176:Protein Biosynthesis
chemical_list: D018387:Codon, Initiator; D003596:Cytosine