The effects of benzoate, cyclohex-1-ene carboxylate, and cyclohexane carboxylate on biotransformation of o-phthalate in sediment slurries under sulfate-reducing conditions.
It was found in this study that while addition of the predicted intermediate product benzoate did not, addition of cyclohex-1-ene or cyclohexane carboxylate did inhibit the biotransformation of o-phthalate in sulfate-reducing o-phthalate-adapted sediment slurries under a CO2/H2 atmosphere. Biotransformation rates of benzoate were slightly higher than those of o-phthalate in sediment slurries amended with o-phthalate plus benzoate. By using the most probable number (MPN) method to determine the number of o-phthalate transformers, similar growth dynamics of o-phthalate transformers were observed in sediment slurries amended with or without benzoate. The number of benzoate transformers (1x10(9)cellsg-1 of sediment) remained the same in sediment slurries repeatedly amended with a mixture of o-phthalate and benzoate after their transformation. o-Phthalate transformers decreased about three orders (1x10(9) to 1x10(6)cellsg-1 of sediment) of magnitude after biotransformation of o-phthalate, and remained at the low number thereafter until re-amendment with o-phthalate. o-Phthalate transformers increased to 1x10(8)cellsg-1 of sediment just prior to the onset of biotransformation of the o-phthalate.
Liu
Shiu-Mei
SM
Institute of Marine Biology, National Taiwan Ocean University, 2-Pening Rd., Keelung, Taiwan. smliu@mail.ntou.edu.tw
Lin
Yue-Lu
YL
Chi
Wei-Chiung
WC
D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001565:Benzoates / Q000494:pharmacology; D001711:Biotransformation / Q000187:drug effects; D002245:Carbon Dioxide / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003510:Cyclohexanes / Q000494:pharmacology; D019015:Geologic Sediments / Q000032:analysis* / Q000382:microbiology; D006859:Hydrogen / Q000494:pharmacology; D007700:Kinetics; D008982:Molybdenum / Q000633:toxicity; D010795:Phthalic Acids / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D013624:Taiwan; D014640:Vancomycin / Q000633:toxicity
D001565:Benzoates; D003510:Cyclohexanes; D010795:Phthalic Acids; D012989:Soil Pollutants; C044659:molybdate; D002245:Carbon Dioxide; C032279:phthalic acid; D014640:Vancomycin; D006859:Hydrogen; D008982:Molybdenum