title: Clinical investigation: increased serum stromal derived factor 1 alpha levels in pulmonary tuberculosis.
abstract: Pulmonary tuberculosis, a granulomatous disease, has few serological markers for its activity. Recently, an increased plasma level of stromal derived factor 1 alpha (SDF-1alpha), which can induce strong chemotaxis of cells through its receptor CXCR4, was detected in patients with tuberculosis. In this study we investigated serum SDF-1alpha levels and CXCR4 expression on peripheral blood mononuclear cells (PBMCs). Fifty-five active tuberculosis patients, 30 resolved tuberculosis patients, 27 acute bronchitis patients and 8 healthy volunteers were examined. Histological expression of SDF-1alpha in the tuberculosis lesion and CXCR4 expression of PBMCs were also analysed. Serum SDF-1alpha levels in active tuberculosis patients were significantly higher than other groups. The sensitivity and specificity for the diagnosis of active tuberculosis was 88.5% and 85.3% (cutoff value = 650 pg/ml), respectively. CXCR4 expression levels on PBMCs showed a significant negative correlation with serum SDF-1alpha levels. Inflammatory cells including multinuclear giant cells in the lesion expressed SDF-1alpha. Measurement of serum SDF-1alpha could be a useful screening marker for the identification of active pulmonary tuberuculosis. We propose that interaction of SDF-1alpha and CXCR4 might be involved in the pathogenesis of pulmonary tuberculosis.
authors.affiliation: Third Department of Internal Medicine, Kagoshima University Faculty of Medicine, Kagoshima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: W
authors.initials: W
authors.lastname: Matsuyama
authors.forename: H
authors.initials: H
authors.lastname: Mitsuyama
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: I
authors.initials: I
authors.lastname: Higashimoto
authors.forename: M
authors.initials: M
authors.lastname: Osame
authors.forename: K
authors.initials: K
authors.lastname: Arimura
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D001991:Bronchitis / Q000276:immunology; D016022:Case-Control Studies; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000032:analysis / Q000097:blood*; D018432:Drug Resistance, Multiple; D005260:Female; D005434:Flow Cytometry; D015726:Giant Cells / Q000737:chemistry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D019718:Receptors, CXCR4 / Q000032:analysis; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000188:drug therapy / Q000276:immunology
chemical_list: D015415:Biomarkers; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D019718:Receptors, CXCR4
references.citation: J Immunol. 1999 Dec 1;163(11):5954-63
references.pmid: 10570282
references.citation: Int J Tuberc Lung Dis. 1998 Oct;2(10):778-83
references.pmid: 9783521
references.citation: Immunity. 2000 Feb;12(2):121-7
references.pmid: 10714678
references.citation: Annu Rev Immunol. 2000;18:217-42
references.pmid: 10837058
references.citation: Am J Respir Crit Care Med. 2000 Sep;162(3 Pt 1):1120-2
references.pmid: 10988140
references.citation: Am J Gastroenterol. 2000 Nov;95(11):3157-64
references.pmid: 11095335
references.citation: Infect Immun. 2001 Feb;69(2):810-5
references.pmid: 11159972
references.citation: J Clin Immunol. 2001 Nov;21(6):390-401
references.pmid: 11811784
references.citation: Infect Immun. 2002 Apr;70(4):1684-93
references.pmid: 11895930
references.citation: J Infect Dis. 2002 Oct 1;186(7):922-31
references.pmid: 12232832
references.citation: Curr Mol Med. 2002 Dec;2(8):691-702
references.pmid: 12462390
references.citation: Immunology. 2003 Mar;108(3):365-74
references.pmid: 12603603
references.citation: J Immunol. 2003 Mar 15;170(6):3392-400
references.pmid: 12626600
references.citation: Lab Invest. 2003 May;83(5):665-72
references.pmid: 12746476
references.citation: J Immunol. 2003 Jun 15;170(12):6320-8
references.pmid: 12794165
references.citation: Am J Respir Crit Care Med. 2003 Jun 15;167(12):1676-86
references.pmid: 12626353
references.citation: Rheumatology (Oxford). 2003 Jul;42(7):915-6
references.pmid: 12826719
references.citation: FASEB J. 2003 Jul;17(10):1286-8
references.pmid: 12738814
references.citation: AIDS Res Hum Retroviruses. 2003 Jun;19(6):461-8
references.pmid: 12882655
references.citation: J Immunol. 2004 Feb 15;172(4):2332-40
references.pmid: 14764702
references.citation: Am J Respir Cell Mol Biol. 2004 May;30(5):671-7
references.pmid: 14672915
references.citation: Am J Respir Cell Mol Biol. 1989 Dec;1(6):439-47
references.pmid: 2700306
references.citation: Am Rev Respir Dis. 1990 Sep;142(3):725-35
references.pmid: 2389921
references.citation: JAMA. 1992 Jan 15;267(3):369-73
references.pmid: 1727959
references.citation: Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2305-9
references.pmid: 8134392
references.citation: Clin Infect Dis. 1994 Jul;19(1):26-32
references.pmid: 7948554
references.citation: Arch Intern Med. 1995 Oct 23;155(19):2111-7
references.pmid: 7575072
references.citation: Genomics. 1995 Aug 10;28(3):495-500
references.pmid: 7490086
references.citation: Nature. 1996 Aug 15;382(6592):635-8
references.pmid: 8757135
references.citation: Nature. 1996 Aug 29;382(6594):829-33
references.pmid: 8752280
references.citation: J Exp Med. 1997 Jan 6;185(1):111-20
references.pmid: 8996247
references.citation: J Exp Med. 1996 Sep 1;184(3):1101-9
references.pmid: 9064327
references.citation: Am J Respir Crit Care Med. 1997 Apr;155(4):1474-7
references.pmid: 9105097
references.citation: Annu Rev Immunol. 1997;15:675-705
references.pmid: 9143704
references.citation: J Exp Med. 1997 Jul 7;186(1):139-46
references.pmid: 9207008
references.citation: Blood. 1997 Aug 1;90(3):909-28
references.pmid: 9242519
references.citation: Eur J Immunol. 1997 Jul;27(7):1788-93
references.pmid: 9247593
references.citation: J Immunol. 1997 Aug 15;159(4):1993-2000
references.pmid: 9257866
references.citation: Curr Opin Immunol. 1997 Aug;9(4):551-62
references.pmid: 9287172
references.citation: J Exp Med. 1997 Nov 17;186(10):1793-8
references.pmid: 9362541
references.citation: Infect Immun. 1998 Mar;66(3):1121-6
references.pmid: 9488404
references.citation: J Immunol. 1998 May 1;160(9):4153-7
references.pmid: 9574513
references.citation: Blood. 1998 Jun 15;91(12):4523-30
references.pmid: 9616148
references.citation: Nature. 1998 Jun 11;393(6685):595-9
references.pmid: 9634238
references.citation: J Virol. 1998 Aug;72(8):6406-13
references.pmid: 9658081
references.citation: Blood. 1998 Aug 1;92(3):756-64
references.pmid: 9680341
references.citation: J Biol Chem. 1998 Sep 4;273(36):23169-75
references.pmid: 9722546
references.citation: Am J Respir Cell Mol Biol. 1998 Sep;19(3):513-21
references.pmid: 9730880