Antibiotic susceptibility of bacteria isolated from pasteurized milk and characterization of macrolide-lincosamide-streptogramin resistance genes.
The presence of antibiotic-resistant bacteria in pasteurized milk was detected by plating 18 milk samples on selective media containing beta-lactams, macrolides, or a glycopeptide. Most samples contained gram-positive bacteria that grew on agar plates containing oxacillin, erythromycin, and/or spiramycin. The disk-diffusion method confirmed resistance to erythromycin and/or spiramycin in 86 and 65% of the coryneform bacteria and Micrococcaceae tested, respectively. PCR and sequence analysis revealed the presence of an ermC gene in 2 of the 25 Micrococcaceae strains investigated for their resistance to erythromycin and/or spiramycin. None of the 14 corynebacteria strains resistant to erythromycin and/or spiramycin harbored the erm(X) gene. No gene transfer could be demonstrated between the two erm(C) staphylococcal isolates and recipient strains of Enterococcus faecalis JH2-2 or Staphylococcus aureus 80CR5.
Perrin-Guyomard
Agnès
A
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherche sur les Médicaments Vétérinaires et Désinfectants, Javené BP90203, 35302 Fougères, France. a.guyomard@fougeres.afssa.fr
Soumet
Christophe
C
Leclercq
Roland
R
Doucet-Populaire
Florence
F
Sanders
Pascal
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D003257:Consumer Product Safety; D003352:Corynebacterium / Q000187:drug effects* / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005511:Food Handling / Q000379:methods*; D005798:Genes, Bacterial; D006801:Humans; D055231:Lincosamides; D018942:Macrolides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008835:Micrococcaceae / Q000187:drug effects* / Q000235:genetics; D008892:Milk / Q000382:microbiology*; D025361:Streptogramins / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D055231:Lincosamides; D018942:Macrolides; D025361:Streptogramins