Preparation of polyvinyl alcohol-polyacrylamide composite polymer membrane by gamma-irradiation for entrapment of urease.
Composite polymer membrane of polyvinyl alcohol (PVA) and acrylamide was prepared on cheesecloth support by gamma-irradiation induced free radical polymerization. The enzyme urease was entrapped in the membrane during polymerization and was cross-linked within the matrix using glutaraldehyde. The membranes could be reused a number of times without significant loss of urease activity.
Jha
Sandeep Kumar
SK
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
D'Souza
Stanislaus F
SF
D000180:Acrylic Resins / Q000737:chemistry*; D003188:Composite Resins / Q000138:chemical synthesis* / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005720:Gamma Rays; D005976:Glutaral / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D014510:Urease / Q000378:metabolism*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D003188:Composite Resins; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005976:Glutaral; D011142:Polyvinyl Alcohol; D014510:Urease; D000099166:Polyacrylamides