Rapid assessment of beta-asarone content of Acorus calamus by micellar electrokinetic capillary chromatography.
This report outlines a rapid, reproducible method for the determination of beta-asarone, a known carcinogen, using micellar electrokinetic capillary chromatography (MEKC)-UV-vis absorbance and a simple alcohol extraction. The MEKC method is based on a running buffer comprised of 100 mM sodium dodecyl sulfate (SDS), pH 10. The method is reproducible and provides baseline separation of alpha-asarone and beta-asarone. This protocol was used to determine the beta-asarone content of Acorus calamus rhizome of a diploid variety harvested from the wetlands of the United States and the triploid variety from India obtained commercially. The results indicate raw product that originated from India contained 4.4% w/w beta-asarone, while that from the United States contained 0.2% w/w beta-asarone. Neither sample contained detectable concentrations of alpha-asarone. This is the first report of the use of MEKC to determine asarone in a natural source.
Hanson
Kim M
KM
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
Gayton-Ely
Melissa
M
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
Holland
Lisa A
LA
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
Zehr
Peter S
PS
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
Söderberg
Björn C G
BCG
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
D029023:Acorus / Q000737:chemistry* / Q000235:genetics; D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000302:isolation & purification*; D002273:Carcinogens / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004171:Diploidy; D011123:Polyploidy; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D013654:Taurine / Q000031:analogs & derivatives* / Q000737:chemistry
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D002273:Carcinogens; C012195:asarone; C050927:2-(N-cyclohexylamino)ethanesulfonic acid; D013654:Taurine; D012967:Sodium Dodecyl Sulfate
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