Transforming growth factor-beta1 induces LMO7 while enhancing the invasiveness of rat ascites hepatoma cells.
We have previously shown that transforming growth factor-beta1 (TGF-beta1) markedly stimulates the invasive capacity of rat ascites hepatoma AH130 W1 cells in vitro and in vivo. A differential hybridization procedure was used to isolate genes that were specifically up-regulated in TGF-beta1 treated W1 cells. Among ten independent cDNA clones, we focused on LMO7 and a variant isoform, LMO7S, that was generated by alternative splicing. LMO7 had PDZ and LIM domains, while LMO7S had only PDZ domain. TGF-beta1 up-regulated expression levels of LMO7 and LMO7S. LMO7 expression was up-regulated in the highly metastatic clone MM1.
Nakamura
Hiroyuki
H
Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan. nakamura-hi@mc.pref.osaka.jp
Mukai
Mutsuko
M
Komatsu
Keiko
K
Tanaka-Okamoto
Miki
M
Itoh
Yu
Y
Ishizaki
Hiroyoshi
H
Tatsuta
Masaharu
M
Inoue
Masahiro
M
Miyoshi
Jun
J
D000818:Animals; D001201:Ascites / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D008113:Liver Neoplasms / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D051381:Rats; D014157:Transcription Factors / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D018398:Homeodomain Proteins; C490020:LMO7 protein, rat; C508962:Tgfb1 protein, rat; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1