[Progress in detection methods of single nucleotide polymorphisms].
Single nucleotide polymorphism (SNP) is the third generation genetic marker. SNP detection now is becoming increasingly important means in molecular diagnostics, clinical assay and novel drug development. It plays an essential role in drug resistance and anaphylactic reaction and has the importance in theoretical studies of gene location, hereditary diseases and human origin. With the accomplishment of human genome sequencing, the genotyping and discovering of SNP are becoming hot subjects in genetics and biomedicine researches. The methods for SNP detection were renewed rapidly and developed fast in past few years. In this review, several newly established detection methods including gene chip, molecular probe, fluorescence polarization and resonance, mass spectrometry, and bacterial magnetic particle are discussed. It could be expected that an accurate and sensitive, simple and easy-to-handle SNP technology with low cost and high throughput will be available on the basis of research breakthroughs of biochemistry, engineering and analytic software.
Zhao
Guang-Rong
GR
Department of Pharmaceutical Engineering, College of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. grzhao@tju.edu.cn
Yang
Fan
F
Yuan
Ying-Jin
YJ
Gao
Xiu-Mei
XM
Zhang
Jun-Ping
JP
D005454:Fluorescence Polarization; D013058:Mass Spectrometry; D015335:Molecular Probes; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
D015335:Molecular Probes