title: Comparison of chemical characteristics of three soybean cysteine proteinase inhibitors.
abstract: Three recombinant soybean cysteine proteinase inhibitors (rSCPIs) L1, R1, and N2 were chemically characterized. These inhibitors have the potential to inhibit the growth and development of three major agricultural crop pests known to utilize cysteine proteinases (CPs) for protein digestion: Western corn rootworm, Colorado potato beetle, and cowpea weevil. Characterization data obtained show differences between the inhibitors and will be needed to consider the use of rSCPIs to create insect resistance in plants.
authors.affiliation: Department of Entomology, Purdue University, 901 West State Street, West Lafayette, Indiana 47907-2089, USA.
authors.forename: S
authors.initials: S
authors.lastname: Lalitha
authors.forename: Richard E
authors.initials: RE
authors.lastname: Shade
authors.forename: Larry M
authors.initials: LM
authors.lastname: Murdock
authors.forename: P Michael
authors.initials: PM
authors.lastname: Hasegawa
authors.forename: Ray A
authors.initials: RA
authors.lastname: Bressan
authors.forename: S Suzanne
authors.initials: SS
authors.lastname: Nielsen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera / Q000201:enzymology; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D013025:Glycine max / Q000201:enzymology*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D011994:Recombinant Proteins; D010447:Peptide Hydrolases