title: Human neuroglobin protein in cerebrospinal fluid.
abstract: BACKGROUND: Neuroglobin is a hexacoordinated member of the globin family of proteins. It is predominantly localized to various brain regions and retina where it may play a role in protection against ischemia and nitric oxide-induced neural injury. Cerebrospinal fluid was collected from 12 chronic regional or systemic pain and 5 control subjects. Proteins were precipitated by addition of 50% 0.2 N acetic acid, 50% ethanol, 0.02% sodium bisulfite. The pellet was extensively digested with trypsin. Peptides were separated by capillary liquid chromatography using a gradient from 95% water to 95% acetonitrile in 0.2% formic acid, and eluted through a nanoelectrospray ionization interface into a quadrapole - time-of-flight dual mass spectrometer (QToF2, Waters, Milford, MA). Peptides were sequenced (PepSeq, MassLynx v3.5) and proteins identified using MASCOT (R). RESULTS: Six different neuroglobin peptides were identified in various combinations in 3 of 9 female pain subjects, but none in male pain, or female or male control subjects. CONCLUSION: This is the first description of neuroglobin in cerebrospinal fluid. The mechanism(s) leading to its release in chronic pain states remain to be defined.
authors.affiliation: Division of Rheumatology, Immunology & Allergy, Georgetown University, Washington DC 20007-2197, USA. bc48@georgetown.edu.
authors.forename: Begona
authors.initials: B
authors.lastname: Casado
authors.forename: Lewis K
authors.initials: LK
authors.lastname: Pannell
authors.forename: Gail
authors.initials: G
authors.lastname: Whalen
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Clauw
authors.forename: James N
authors.initials: JN
authors.lastname: Baraniuk
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