[Studies on the packings of high performance strong cation exchange chromatography for the separation of proteins].
In this paper, the studies on the synthesis, chromatographic characteristics and application of new packings of high performance strong cation exchange chromatography with sulfonic group for the separation of proteins have been reported. The silica with the end hydroxyl group reacted with chlorosulfonic acid and thus packings for high performance strong cation exchange chromatography were obtained. The recovery of bioactivity for lysozyme of these high resolution packings is over 76%. The packings can be used for the separation of proteins and biotechnological products.
Chang
J
J
Department of Chemistry, Northwest University, Xi'an, 710069.
Guo
Z
Z
D000818:Animals; D002411:Cation Exchange Resins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D002411:Cation Exchange Resins; D011506:Proteins