Magnetic hydrophilic methacrylate-based polymer microspheres for genomic DNA isolation.
Carboxyl groups containing magnetic and non-magnetic microspheres were used in solid-phase reversible immobilization (SPRI) of genomic DNA. Magnetic non-porous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)--P(HEMA-co-EDMA), poly(glycidyl methacrylate)--PGMA and P(HEMA-co-GMA) microspheres with hydrophilic properties were prepared by dispersion copolymerization of the respective monomers in the presence of colloidal iron oxides. DNA from chicken erythrocytes and DNA isolated from bacterial cells of Bifidobacterium longum was used for testing of adsorption/desorption properties of magnetic microspheres. The occurrence of false negative results in polymerase chain reaction (PCR) caused by the presence of extracellular inhibitors in DNA samples has been solved using SPRI. The P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres were used for isolation of DNA from different dairy products followed by PCR identification of Bifidobacterium strains.
Krízová
Jana
J
Masaryk University Brno, Faculty of Science, Department of Microbiology, Tvrdého 14, 60200 Brno, Czech Republic.
Spanová
Alena
A
Rittich
Bohuslav
B
Horák
Daniel
D
D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D016678:Genome; D008280:Magnetics; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D016133:Polymerase Chain Reaction
D008689:Methacrylates; D004247:DNA