Auxin-dependent cell division and cell elongation. 1-Naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid activate different pathways.
During exponential phase, the tobacco (Nicotiana tabacum) cell line cv Virginia Bright Italia-0 divides axially to produce linear cell files of distinct polarity. This axial division is controlled by exogenous auxin. We used exponential tobacco cv Virginia Bright Italia-0 cells to dissect early auxin signaling, with cell division and cell elongation as physiological markers. Experiments with 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) demonstrated that these 2 auxin species affect cell division and cell elongation differentially; NAA stimulates cell elongation at concentrations that are much lower than those required to stimulate cell division. In contrast, 2,4-D promotes cell division but not cell elongation. Pertussis toxin, a blocker of heterotrimeric G-proteins, inhibits the stimulation of cell division by 2,4-D but does not affect cell elongation. Aluminum tetrafluoride, an activator of the G-proteins, can induce cell division at NAA concentrations that are not permissive for division and even in the absence of any exogenous auxin. The data are discussed in a model where the two different auxins activate two different pathways for the control of cell division and cell elongation.
Campanoni
Prisca
P
Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy. campanoni@ibba.cnr.it
Nick
Peter
P
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D049108:Cell Enlargement / Q000187:drug effects*; D002460:Cell Line; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D009280:Naphthaleneacetic Acids / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000166:cytology*
D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D011956:Receptors, Cell Surface; C083845:endoplasmic reticulum auxin-binding protein 4, Zea mays; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid
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