Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms.
Histone phosphorylation influences transcription, chromosome condensation, DNA repair and apoptosis. Previously, we showed that histone H3 Ser10 phosphorylation (pSer10) by the yeast Snf1 kinase regulates INO1 gene activation in part via Gcn5/SAGA complex-mediated Lys14 acetylation (acLys14). How such chromatin modification patterns develop is largely unexplored. Here we examine the mechanisms surrounding pSer10 at INO1, and at GAL1, which herein is identified as a new regulatory target of Snf1/pSer10. Snf1 behaves as a classic coactivator in its recruitment by DNA-bound activators, and in its role in modifying histones and recruiting TATA-binding protein (TBP). However, one important difference in Snf1 function in vivo at these promoters is that SAGA recruitment at INO1 requires histone phosphorylation via Snf1, whereas at GAL1, SAGA recruitment is independent of histone phosphorylation. In addition, the GAL1 activator physically interacts with both Snf1 and SAGA, whereas the INO1 activator interacts only with Snf1. Thus, at INO1, pSer10's role in recruiting SAGA may substitute for recruitment by DNA-bound activator. Our results emphasize that histone modifications share general functions between promoters, but also acquire distinct roles tailored for promoter-specific requirements.
Lo
Wan-Sheng
WS
Division of Biological Sciences and UCSD Cancer Center, University of California, San Diego, CA, USA.
Gamache
Eric R
ER
Henry
Karl W
KW
Yang
David
D
Pillus
Lorraine
L
Berger
Shelley L
SL
D001693:Biological Transport, Active; D018797:Cell Cycle Proteins / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D008954:Models, Biological; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D012694:Serine / Q000737:chemistry; D035181:TATA-Box Binding Protein / Q000031:analogs & derivatives* / Q000378:metabolism*; D015533:Transcriptional Activation
D018797:Cell Cycle Proteins; D006657:Histones; C115017:SPT15 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D012694:Serine; D007294:Inositol; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; D005690:Galactose
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