title: Vasorelaxant effect of resin-based, single-bottle dentin bonding systems.
abstract: Single-bottle dentin bonding systems are currently in wide use. Because these materials are sometimes inadvertently placed on microscopic pulp exposures while at other times deliberately on frank exposures, their effects on pulpal soft tissues need to be evaluated. The present study assessed the vascular effects of 3M Single Bond (3MSB) and Prime & Bond NT (PBNT), using rat aortic ring preparations. It is hypothesized that these bonding agents induce relaxation of these preparations. Both 3MSB and PBNT caused endothelium-dependent and -independent relaxations in a concentration-dependent manner. The endothelium-dependent relaxation was associated with the release of nitric oxide. However, the responses to both agents did not involve the generation of prostanoids or KATP channel activation. At relatively low concentrations, the responses of endothelium-denuded tissues to 3MSB were greater than those to PBNT, indicating certain differences in the vascular action between these products. The data suggest that 3MSB and PBNT interfere with vascular function by causing vasorelaxation via mechanisms occurring in the smooth muscle and endothelium, including the release of nitric oxide. Among others, this effect may promote bleeding if these adhesives are placed on pulp exposures.
authors.affiliation: Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1128, USA. wabebe@mail.mcg.edu
authors.forename: Worku
authors.initials: W
authors.lastname: Abebe
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Fred A
authors.initials: FA
authors.lastname: Rueggeberg
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis; D011109:Polymethacrylic Acids / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D019279:Resin Cements / Q000633:toxicity*; D014664:Vasodilation
chemical_list: D017220:Dentin-Bonding Agents; D004791:Enzyme Inhibitors; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C115612:single bond; D009569:Nitric Oxide; D017438:Bisphenol A-Glycidyl Methacrylate; D019331:NG-Nitroarginine Methyl Ester