Serine dehydratase expression decreases in rat livers injured by chronic thioacetamide ingestion.
Serine dehydratase (SerDH) is a gluconeogenic enzyme involved in the catabolism of serine, which is regulated by the composition of their diet and their hormonal status in rats. This study examines how chronic injury caused to the liver of rats by the ingestion of thioacetamide (TAA) affects SerDH protein, mRNA levels, enzyme kinetics and its tissue location. After 97 days' oral intake of TAA, the activity of SerDH at all substrate concentrations assayed was about 60% lower than in controls. No significant differences in Km values were found between the treated group and controls. Immunoblotting and immunohistochemistry revealed a significant reduction in the level of SerDH protein in the livers of the treated rats. SerDH was detected specifically in the periportal zone of the hepatic acinus and this location did not change in response to TAA treatment. The level of SerDH mRNA, quantified by reverse transcription and polymerase chain reaction, was significantly lower in treated rats than in the controls. The present findings suggest that the SerDH expression is rendered to be down regulatory during chronic liver injury induced by TAA. These results enhance our understanding about the biochemical mechanisms implied in the control and integration of serine catabolism during liver injury in rat.
López-Flores
Inmaculada
I
Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, Jaén, Spain.
Barroso
Juan B
JB
Valderrama
Raquel
R
Esteban
Francisco J
FJ
Martínez-Lara
Esther
E
Luque
Francisco
F
Peinado
M Angeles
MA
Ogawa
Hirofumi
H
Lupiáñez
José A
JA
Peragón
Juan
J
D000818:Animals; D015536:Down-Regulation / Q000187:drug effects*; D012695:L-Serine Dehydratase / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000378:metabolism; D013853:Thioacetamide / Q000633:toxicity*
D012333:RNA, Messenger; D013853:Thioacetamide; D012694:Serine; D012695:L-Serine Dehydratase
Biochem Pharmacol. 1986 Aug 15;35(16):2655-61
2874812
Adv Enzyme Regul. 1984;22:325-400
6089514
Physiol Rev. 1989 Jul;69(3):708-64
2664826
Gastroenterology. 1998 Dec;115(6):1530-40
9834282
Arch Biochem Biophys. 1995 Feb 1;316(2):844-50
7864642
J Nutr Sci Vitaminol (Tokyo). 2003 Feb;49(1):33-9
12882394
J Biol Chem. 1964 Jun;239:1783-8
14213351
Clin Investig. 1992 Jun;70(6):478-86
1392415
Mol Endocrinol. 1991 Nov;5(11):1661-8
1779969
J Pharmacol Exp Ther. 1977 Feb;200(2):439-48
839448
Oncology. 1981;38(4):249-53
6264362
J Biol Chem. 1999 Jun 4;274(23):16020-7
10347151
Exp Pathol. 1986;30(2):109-17
3780937
Arch Biochem Biophys. 1999 Feb 15;362(2):317-24
9989941
J Biol Chem. 1990 Aug 25;265(24):14407-13
2387860
Lancet. 1980 Mar 1;1(8166):454-6
6102186
Biochem J. 2003 May 1;371(Pt 3):653-61
12534373
J Biol Chem. 1971 Apr 25;246(8):2626-32
4994928
J Biol Chem. 1991 Oct 25;266(30):20412-7
1939096
Neurosci Lett. 1988 Feb 29;85(2):169-71
3374833
Arch Biochem Biophys. 1991 Aug 1;288(2):562-6
1654838
J Hepatol. 1993 Aug;19(1):64-70
8301044
Arch Biochem Biophys. 2003 Apr 15;412(2):216-22
12667485
Toxicology. 1996 Jan 8;106(1-3):197-206
8571392
J Cell Biochem. 2002;87(1):16-28
12210718
Hepatology. 1995 Jan;21(1):199-206
7806155
FEBS Lett. 1975 Jul 15;55(1):202-5
1140417
Exp Clin Endocrinol Diabetes. 1995;103(2):63-74
7553077
J Biochem. 1965 Apr;57:506-13
14318080
Anal Biochem. 1976 May 7;72:248-54
942051
Eur J Biochem. 1974 Apr 16;43(3):609-16
4831976
Anal Biochem. 1987 Apr;162(1):156-9
2440339
Am J Physiol. 1998 Jun;274(6 Pt 2):R1578-87
9608011
J Biol Chem. 1968 Jun 10;243(11):3057-66
4968185
J Biol Chem. 1999 Apr 30;274(18):12855-60
10212273
Histochem J. 1995 May;27(5):380-7
7657557
Arch Biochem Biophys. 1994 Jan;308(1):285-91
8311466
J Nutr. 2001 Jan;131(1):91-8
11208943
Carcinogenesis. 1988 Mar;9(3):387-94
3345580