title: Inhibitory effects of naringenin on tumor growth in human cancer cell lines and sarcoma S-180-implanted mice.
abstract: We have investigated the effect of naringenin (NGEN) on tumor growth in various human cancer cell lines and sarcoma S-180-implanted mice. NGEN showed cytotoxicity in cell lines derived from cancer of the breast (MCF-7, MDA-MB-231), stomach (KATOIII, MKN-7), liver (HepG2, Hep3B, Huh7), cervix (Hela, Hela-TG), pancreas (PK-1), and colon (Caco-2) as well as leukemia (HL-60, NALM-6, Jurkat, U937). NGEN-induced cytotoxicity was low in Caco-2 and high in leukemia cells compared to other cell lines. NGEN dose-dependently induced apoptosis, with hypodiploid cells detected in both Caco-2 and HL-60 by flow cytometric analysis. In vivo, NGEN inhibited tumor growth in sarcoma S-180-implanted mice, following intraperitoneal or peroral injection once a day for 5 d. Naringin (NG) also inhibited tumor growth by peroral injection but not intraperitoneal injection. NGEN, one of the most abundant flavonoids in citrus fruits, may have a potentially useful inhibitory effect on tumor growth.
authors.affiliation: Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical University, Komatsushima, Sendai, Japan. syu-kan@tohoku-pahrm.ac.jp
authors.forename: Syu-Ichi
authors.initials: S
authors.lastname: Kanno
authors.forename: Ayako
authors.initials: A
authors.lastname: Tomizawa
authors.forename: Takako
authors.initials: T
authors.lastname: Hiura
authors.forename: Yuu
authors.initials: Y
authors.lastname: Osanai
authors.forename: Ai
authors.initials: A
authors.lastname: Shouji
authors.forename: Mayuko
authors.initials: M
authors.lastname: Ujibe
authors.forename: Takaharu
authors.initials: T
authors.lastname: Ohtake
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kimura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D044950:Flavanones / Q000627:therapeutic use*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D044950:Flavanones; D006131:Growth Inhibitors; C005273:naringenin