Effects of sub-minimum inhibitory concentration antibiotic levels and temperature on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus.
The objective of this study was to determine the effects of sub-minimum inhibitory concentrations (sub-MICs) of 2 veterinary antibiotic preparations, chlortetracycline (CTC) and chlortetracycline-sulfamethazine (CTC + SMZ), on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus at normal and febrile body temperatures. Sub-minimum inhibitory concentrations of both antibiotics reduced the growth rates of M. haemolytica and H. somnus. Growth of both species was not inhibited when grown at 41 degrees C compared to 37 degrees C. There was no detectable consistent effect of antibiotic or temperature on outer membrane protein expression for either species. Our study indicates that sub-MIC levels of CTC and CTC + SMZ markedly impair growth of clinical M. haemolytica and H. somnus isolates, potentially allowing more effective host clearance during infection.
Reeks
Brenda Y
BY
College of Veterinary Medicine, Mississippi State University, Mississippi 39762, USA.
Champlin
Franklin R
FR
Paulsen
Daniel B
DB
Scruggs
Daniel W
DW
Lawrence
Mark L
ML
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000187:drug effects* / Q000378:metabolism; D002751:Chlortetracycline / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D015964:Gene Expression Regulation, Bacterial; D044138:Haemophilus somnus / Q000187:drug effects* / Q000254:growth & development; D007700:Kinetics; D016978:Mannheimia haemolytica / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D013418:Sulfamethazine / Q000494:pharmacology*; D013696:Temperature
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D013418:Sulfamethazine; D002751:Chlortetracycline
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