Stimulated resistin expression in white adipose of rats with bile duct ligation-induced liver cirrhosis: relationship to cirrhotic hyperinsulinemia and increased tumor necrosis factor-alpha.
Resistin, an adipose-derived polypeptide hormone, is proposed as a candidate of insulin resistance, although its roles in inhibiting adipogenesis and in inflammation have also been suggested. Liver cirrhosis is characterized by elevated circulating proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha), hyperinsulinemia and insulin resistance. The study aimed to examine resistin expression and its association with insulin and TNF-alpha in a cirrhotic rat model using bile duct ligation (BDL). The BDL-induced cirrhotic rats showed significantly lower fat mass, insulin sensitivity and elevated plasma insulin and TNF-alpha compared to sham animals. In addition, epididymal TNF-alpha and resistin mRNA and protein levels were higher in cirrhotic rats. In normal control rats, in vivo insulin infusion and ex vivo administration of TNF-alpha to cultured fat pads increased resistin gene expression significantly. These results implied that hyperinsulinemia and increased TNF-alpha levels might upregulate adipose resistin gene in BDL-induced liver cirrhosis. Further study is necessary to document the role of resistin in metabolic abnormalities of liver cirrhosis.
Lin
Shih-Yi
SY
Division of Endocrinology and Metabolism, Department of Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.
Sheu
Wayne Huey-Herng
WH
Chen
Wen-Yin
WY
Lee
Fa-Yauh
FY
Huang
Ching-Jang
CJ
D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001652:Bile Ducts; D001823:Body Composition; D005786:Gene Expression Regulation / Q000187:drug effects*; D006729:Hormones, Ectopic / Q000032:analysis / Q000235:genetics*; D006946:Hyperinsulinism / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000494:pharmacology*
D006729:Hormones, Ectopic; D007328:Insulin; D012333:RNA, Messenger; D052243:Resistin; C499622:Retn protein, rat; D014409:Tumor Necrosis Factor-alpha