[Pseudolaric acid B induces human melanoma A375-S2 cell apoptosis in vitro].
OBJECTIVE
To study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells.


METHODS
MTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused.


RESULTS
Pseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased.


CONCLUSIONS
The major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.
Gong
Xian-Feng
XF
China-Japan Research Institute of Medical and Pharmaceutical University, Shenyang 110016, China.
Wang
Min-Wei
MW
Tashiro
Shin-ichi
S
Onodera
Satoshi
S
Ikejima
Takashil
T
D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D028201:Pinaceae / Q000737:chemistry; D011506:Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
D051017:Apoptosis Regulatory Proteins; C494826:BAX protein, human; C494811:BCL2L1 protein, human; D004224:Diterpenes; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C109664:caspase-activated DNase inhibitor; C058391:pseudolaric acid B