Production of bacteriocins by coagulase-negative staphylococci involved in bovine mastitis.
In the present study, 188 coagulase-negative Staphylococcus (CNS) strains were isolated from bovine mastitis cases from 56 different Brazilian dairy herds, located in the Southeast region of the country, and were tested for antimicrobial substance production. Twelve CNS strains (6.4%) exhibited antagonistic activity against a Corynebacterium fimi indicator strain. Most antimicrobial substances were sensitive to proteolytic enzymes suggesting that they might be bacteriocins (Bac). Amongst the CNS producers, six were identified as S. epidermidis, two as S. simulans, two as S. saprophyticus, one as S. hominis and one as S. arlettae. Plasmid profile analysis of these strains revealed the presence of at least one plasmid. The Bac(+) strains presented either no or few antibiotic resistance phenotypes. Three strains were shown to produce a bacteriocin either identical or similar to aureocin A70, a bacteriocin previously isolated from an S. aureus strain isolated from food. The remaining Bac(+) strains produce antimicrobial peptides that seem to be distinct from the best characterised staphylococcal bacteriocins described so far. Some of them were able to inhibit Listeria monocytogenes, an important food-borne pathogen, and several strains of Streptococcus agalactiae associated with bovine mastitis, suggesting a potential use of these bacteriocins either in the prevention or in the treatment of streptococcal mastitis.
dos Santos Nascimento
Janaína
J
Instituto de Microbiologia Prof. Paulo de Góes, Departamento de Microbiologia Geral, Universidade do Brazil (UFRJ), CCS, Bloco I, sala I-1-059, Cidade Universitária, 21941-590 Rio de Janeiro, RJ, Brazil.
Fagundes
Patricia Carlin
PC
de Paiva Brito
Maria Aparecida Vasconcelos
MA
dos Santos
Kátia Regina Netto
KR
do Carmo de Freire Bastos
Maria
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000378:metabolism; D015139:Blotting, Southern / Q000662:veterinary; D002417:Cattle; D003030:Coagulase / Q000172:deficiency; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005260:Female; D008089:Listeria monocytogenes / Q000254:growth & development; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D010447:Peptide Hydrolases / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013203:Staphylococcal Infections / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003030:Coagulase; D004269:DNA, Bacterial; D010447:Peptide Hydrolases