title: Cytotoxicity of three-dimensionally ordered macroporous sol-gel bioactive glass (3DOM-BG).
abstract: In this study, 80% SiO(2)-20% CaO (mole fraction) three-dimensionally ordered macroporous sol-gel bioactive glass (3DOM-BG, average pore size: 345 nm) particles were prepared and characterized. Since the 3DOM-BGs have a novel microstructure and ion-releasing profile, the cytotoxicity of 3DOM-BG particles was tested. The cytotoxicity tests were performed using MC3T3-E1 osteoblast-like cells: (1) Wst-1 assay for cell viability after culture in extracts from 3DOM-BG particles; (2) phase contrast microscopy for cell morphology after culture with 3DOM-BG particles; and (3) fluorescence microscopy for imaging cells cultured directly on 3DOM-BG particles. The results showed that 3DOM-BG particles were not cytotoxic, and that cells attached, spread and proliferated on and around 3DOM-BG particles.
authors.affiliation: Polymers Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899-8545, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Newell R
authors.initials: NR
authors.lastname: Washburn
authors.forename: Carl G
authors.initials: CG
authors.lastname: Simon
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D002470:Cell Survival; D005898:Glass; D051379:Mice; D008856:Microscopy, Fluorescence
chemical_list: D001672:Biocompatible Materials