title: Stimulation of human trophoblast invasion by placental growth hormone.
abstract: A critical step in establishment of human pregnancy is the invasion of the uterus wall by the extravillous cytotrophoblast (EVCT), a process regulated by multiple autocrine and paracrine factors. Hormones belonging to the GH/prolactin family are expressed at the maternofetal interface. Because they are involved in cell motility in various models, we examined the possible regulatory role of human placental GH (hPGH) in EVCT invasiveness. By using an in vitro invasion model, we found that EVCT isolated from first-trimester chorionic villi and cultured on Matrigel secreted hPGH and expressed human GH receptor (hGHR). These data were confirmed by in situ immunohistochemistry. EVCT expressed the full-length and truncated forms of hGHR, and the Janus kinase-2/signal transducer and activator of transcription factor-5 signaling pathway was activated in EVCT by hPGH treatment. Strong hPGH and hGHR expression was observed when EVCT invaded Matrigel and moved through the pores of the filter on which they were cultured. hPGH stimulated EVCT invasiveness, and this effect was inhibited by a Janus kinase-2 inhibitor. Interestingly, hPGH was more efficient than pituitary GH in stimulating EVCT invasiveness. These results offer the first evidence for a placental role of hPGH and suggest an autocrine/paracrine role of hPGH in the regulation of trophoblast invasion.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 427, Faculté des Sciences Pharmaceutiques et Biologiques, Université René Descartes, 75270 Paris, France. mlacroix@jouy.inra.fr
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Lacroix
authors.forename: Jean
authors.initials: J
authors.lastname: Guibourdenche
authors.forename: Thierry
authors.initials: T
authors.lastname: Fournier
authors.forename: Ingrid
authors.initials: I
authors.lastname: Laurendeau
authors.forename: Ahmed
authors.initials: A
authors.lastname: Igout
authors.forename: Vincent
authors.initials: V
authors.lastname: Goffin
authors.forename: Jacques
authors.initials: J
authors.lastname: Pantel
authors.forename: Vassilis
authors.initials: V
authors.lastname: Tsatsaris
authors.forename: Daniele
authors.initials: D
authors.lastname: Evain-Brion
mesh_terms: D002478:Cells, Cultured; D002824:Chorionic Villi; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000502:physiology; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D053614:Janus Kinase 2; D008894:Milk Proteins / Q000378:metabolism; D008954:Models, Biological; D010920:Placenta / Q000502:physiology; D010926:Placental Hormones / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D010928:Placental Lactogen / Q000235:genetics; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D011986:Receptors, Somatotropin / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism; D014162:Transfection; D014327:Trophoblasts / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; C064980:GH2 protein, human; D008894:Milk Proteins; D010926:Placental Hormones; D011518:Proto-Oncogene Proteins; D011986:Receptors, Somatotropin; D011993:Recombinant Fusion Proteins; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D013006:Growth Hormone; D010928:Placental Lactogen; D011505:Protein-Tyrosine Kinases; C507924:JAK2 protein, human; D053614:Janus Kinase 2