Molecular markers for human colon cancer in stool and blood identified by RT-PCR.
There is a need for sensitive and specific diagnostic and prognostic molecular markers which can monitor early patterns of gene expression in non-invasive exfoliated colonocytes shed in the stool, and aggression in carcinoma cells in blood of resected colorectal cancer patients. RNA-based detection methods are more comprehensive than either DNA- or protein-based methods. By routinely and systematically being able to perform quantitative gene expression studies on non-invasive samples using carefully selected tumor-specific colon cancer genes, we can quantitatively and accurately monitor changes at various stages in the neoplastic process, allowing for surgical and/or other therapies, and thus, decrease mortality from colorectal cancer.
Ahmed
Farid E
FE
Department of Radiation Oncology, LSB 014, Leo W. Jenkins Cancer Center, The Brody School of Medicine (BSOM) at East Carolina University (ECU), Greenville, NC 27858, USA. ahmedf@mail.ecu.edu
Vos
Paul
P
D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D018189:Immunomagnetic Separation; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm