Characterization of HER1 (c-erbB1) status in locally advanced breast cancer using fluorescence in situ hybridization and immunohistochemistry.
Epidermal growth factor receptor (EGFR) is a 170-kDa transmembrane glycoprotein encoded by the HER1 protooncogene, located at 7p12. This receptor is related to the pathogenesis of breast cancer. The aim of this study was to analyze the status of HER1 using fluorescence in situ hybridization (FISH) and immunohistochemistry in a series of 48 patients with locally advanced breast cancer (LABC). Before neoadjuvant chemotherapy, core biopsies were taken from patients with LABC and were processed into paraffin blocks. Biopsies were then studied using FISH with a HER1 probe (Vysis, Downers Grove, Ill., USA). They were also analyzed immunohistochemically using two different EGFR antibodies from DakoCytomation (Denmark, A/S) and from Zymed (San Francisco, Calif., USA). HER1 amplifications were not found, although 31% of the cases presented aneusomy of chromosome 7. Only 2 cases presented EGFR expression. LABC presented a low level of EGFR expression. HER1 amplification was not present in LABC, although the polysomy of chromosome 7 was a common finding.
Corzo
Cristina
C
Laboratori de Citogenètica i Biologia molecular, Unitat de Recerca Traslacional en Tumors Sólids, Hospital del Mar, IMAS, Barcelona, Spain. E0062@imas.imim.es
Tusquets
Ignacio
I
Salido
Marta
M
Corominas
Josep Maria
JM
Bellet
Meritxell
M
Suarez
Marta
M
Baró
Teresa
T
Fabregat
Xavier
X
Serrano
Sergio
S
Solé
Francesc
F
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002897:Chromosomes, Human, Pair 7; D066246:ErbB Receptors / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D018628:Gene Dosage; D018773:Genes, erbB-1; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D009367:Neoplasm Staging; D013030:Spain
D066246:ErbB Receptors