[Effect of interleukin-6 on gene expression of certain cytokines during wound healing process of mouse skin].
To examine the effect of the interlukin-6 (IL-6) in wound healing process, gene expression profiles of cytokines including interlukin-1alpha (IL-1alpha), interlukin-1beta (IL-1beta), keratinocyte chemoattractant (KC), macrophage inflammatory protein-1alpha (MIP-1alpha), and macrophage inflammatory protein-2 (MIP-2) during the skin wound healing on the IL-6(+/+) and IL-6(-/-) mice were detected using immunochemical staining and RT-PCR methods at different phases after wound. The results showed that these cytokines were expressed at early phage after wound, and a expressing peak were found on the third day after wound, and decreased on the sixth day after wound. Meanwhile, on the third and sixth day after wound, all the levels of the five cytokines expressed of the IL6(-/-) mice were significantly lower than those of the IL-6(+/+) mice, but on the first day after wound, only the levels of MIP-1alpha and KC of the IL-6(-/-) mice were lower. The process of skin wound healing was later in IL-6(-/-) mice than that in IL-6(+/+) mice, but it was complete in IL-6(-/-) mice. These results showed that IL-6 induce expression of the other five cytokines detected, and advance the repair of the wound on skin of mice, but in the mice of IL-6 deletion, the wound healing process was not disturbed significantly.
Lin
Zi-Qing
ZQ
Faculty of Forensic Medicine, China Criminal Police College, Shenyang 110035, China. lziqing@ccpc.edu.cn
Dong
Yu-Zhen
YZ
Zhang
Xiao-Dong
XD
Wang
Tong
T
Sun
Kai-Lai
KL
Niu
Wen-You
WY
D000818:Animals; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics / Q000502:physiology*; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012867:Skin / Q000293:injuries*; D013997:Time Factors; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger