title: Studies in macaques on cross-clade T cell responses elicited by a DNA/MVA AIDS vaccine, better conservation of CD8 than CD4 T cell responses.
abstract: One of the unknowns faced by an HIV/AIDS vaccine is the ability of a single clade vaccine to protect against the multiple genetic subtypes and recombinant forms of HIV-1 present in the current pandemic. Here, we use a macaque model to investigate the ability of our clade B vaccine that consists of DNA priming and modified vaccinia Ankara (MVA) virus boosting to elicit T cell responses that recognize an A/G recombinant of HIV-1. To test for cross-reactive T cells, intracellular cytokine staining was conducted using five pools of Gag and six pools of Env peptides representing B or A/G sequences. Studies using the peptide pools revealed essentially complete conservation of the CD8 response but only approximately 50% conservation of the CD4 response. Thus, the ability of an HIV vaccine for one clade to protect against other clades may be more limited by the ability to provide CD4 T cell help than the ability to elicit CD8 effector functions.
authors.affiliation: Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia 30329, USA.
authors.forename: James M
authors.initials: JM
authors.lastname: Smith
authors.forename: Rama Rao
authors.initials: RR
authors.lastname: Amara
authors.forename: Linda S
authors.initials: LS
authors.lastname: Wyatt
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Ellenberger
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: James G
authors.initials: JG
authors.lastname: Herndon
authors.forename: Milloni
authors.initials: M
authors.lastname: Patel
authors.forename: Sunita
authors.initials: S
authors.lastname: Sharma
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Chennareddi
authors.forename: Sal
authors.initials: S
authors.lastname: Butera
authors.forename: Janet
authors.initials: J
authors.lastname: McNicholl
authors.forename: Harold M
authors.initials: HM
authors.lastname: McClure
authors.forename: Bernard
authors.initials: B
authors.lastname: Moss
authors.forename: Harriet L
authors.initials: HL
authors.lastname: Robinson
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003429:Cross Reactions; D015497:HIV-1 / Q000276:immunology*; D008251:Macaca; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic