title: Disseminated Acanthamoeba sp. infection in a dog.
abstract: Several species of free-living amoebae can cause encephalomyelitis in animals and humans. Disseminated acanthamoebiasis was diagnosed in pyogranulomatous lesions in brain, thyroid, pancreas, heart, lymph nodes, and kidney of a one-year-old dog. Acanthamoeba sp. was identified in canine tissues by conventional histology, by immunofluorescence, by cultivation of the parasite from the brain of the dog that had been stored at -70 degrees C for two months, and by PCR. The sequence obtained from the PCR product from the amoeba from the dog was compared to other sequences in the Acanthamoeba sp. ribosomal DNA database and was determined to be genotype T1, associated with other isolates of Acanthamoeba obtained from granulomatous amebic encephalitis infections in humans.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705-2350, USA. jdubey@anri.barc.usda.gov
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: J E
authors.initials: JE
authors.lastname: Benson
authors.forename: K T
authors.initials: KT
authors.lastname: Blakeley
authors.forename: G C
authors.initials: GC
authors.lastname: Booton
authors.forename: G S
authors.initials: GS
authors.lastname: Visvesvara
mesh_terms: D000048:Acanthamoeba / Q000235:genetics / Q000302:isolation & purification*; D000562:Amebiasis / Q000469:parasitology / Q000662:veterinary*; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D004679:Encephalomyelitis / Q000469:parasitology / Q000662:veterinary*; D017809:Fatal Outcome; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics
chemical_list: D000953:Antigens, Protozoan; D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S