Influence of electrostatic interaction on fibrinogen adsorption on gold studied by imaging ellipsometry combined with electrochemical methods.
Imaging ellipsometry was combined with electrochemical methods for studying electrostatic interactions of protein and solid surfaces. The potential of zero charge for gold-coated silicon wafer/solution interfaces wad determined by AC impedance method. The potential of the gold-coated silicon wafer was controlled at the potential of zero charge, and the adsorption of fibrinogen on the potential-controlled and non-controlled surfaces was measured in real time at the same time by imaging ellipsometry. The effect of electrostatic interaction was studied by comparing the difference between the potential of controlled adsorption and the potential of noncontrolled adsorption. It was shown that the rate of fibrinogen adsorption on the potentiostatic surface was faster than that on the nonpotentiostatic surface. The electrostatic influence on fibrinogen adsorption on the gold-coated silicon wafer was weak, so the hydrophobic interaction should be the major affinity.
Yu
Yong
Y
Laboratory of National Microgravity, Institute of Mechanics, Chinese Academy of Science, Beijing 100080, People's Republic of China.
Jin
Gang
G
D000327:Adsorption; D004563:Electrochemistry; D005340:Fibrinogen / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008027:Light; D055095:Optics and Photonics; D012825:Silicon / Q000737:chemistry; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods; D055672:Static Electricity; D013499:Surface Properties; D013997:Time Factors
D006046:Gold; D005340:Fibrinogen; D012825:Silicon