Formalin hindpaw injection induces changes in the [3H]prazosin binding to alpha1-adrenoceptors in specific regions of the mouse brain and spinal cord.
Involvement of the alpha1-adrenoceptor subtypes in early and late phases of formalin pain was investigated by quantitative in vitro autoradiography in the spinal cord and brain structures of CD-1 mice. Total alpha1-adrenoceptors binding (including all alpha1-adrenoceptor subtypes) was assessed with [3H]prazosin; alpha(1B)-adrenoceptor was assessed with [3H]prazosin in the presence of 10 nM WB4101 to mask remaining alpha1-adrenoceptor subtypes. Early after formalin injection the alpha1-adrenoceptors (mainly alpha1B receptor) binding was reduced in the contralateral hind limb area of the somatosensory cortex and in the secondary motor cortex. A reduction occurred also in the ipsilateral laminae I-III of the spinal cord (both alpha1B- and non-alpha1B-adrenoceptors). Lately an increase of alpha1-adrenoceptors binding (mostly subtypes other than alpha1B) appeared in discrete amygdaloid and thalamic nuclei. These results provide the first description of changes at the level of central alpha1-adrenoceptors' binding during the formalin-induced pain in mice. Their distribution suggests that they may have a functional meaning.
Nalepa
I
I
Department of Biochemistry, Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland. nfnalepa@cyf-kr.edu.pl
Vetulani
J
J
Borghi
V
V
Kowalska
M
M
Przewłocka
B
B
Pavone
F
F
D000679:Amygdala / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002490:Central Nervous System / Q000378:metabolism*; D006614:Hindlimb; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009044:Motor Cortex / Q000378:metabolism; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D010147:Pain Measurement; D011224:Prazosin / Q000378:metabolism* / Q000494:pharmacology; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D013003:Somatosensory Cortex / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D013788:Thalamus / Q000378:metabolism; D014316:Tritium
D018340:Receptors, Adrenergic, alpha-1; D014316:Tritium; D011224:Prazosin
Science. 1987 Jan 2;235(4784):46-52
2432656
Can J Physiol Pharmacol. 1997 Oct-Nov;75(10-11):1203-11
9431444
Naunyn Schmiedebergs Arch Pharmacol. 1995 Jul;352(1):1-10
7477417
Pol J Pharmacol. 2001 Sep-Oct;53(5):475-80
11990065
Trends Pharmacol Sci. 1996 Jul;17(7):245-55
8756183
Brain Res Bull. 1999 Jan 15;48(2):129-41
10230704
Neuropharmacology. 1993 Apr;32(4):367-71
8098863
Anesthesiology. 2002 Feb;96(2):367-74
11818770
Neuroscience. 1993 Nov;57(1):143-51
8278048
J Neurosci. 1990 Aug;10(8):2580-6
1974919
Brain Res. 1998 Apr 20;790(1-2):14-24
9593804
Brain Res. 2002 Nov 29;956(2):339-48
12445704
Prog Neurobiol. 1993 Nov;41(5):565-607
8284437
Mol Pharmacol. 1994 Aug;46(2):221-6
8078485
Anesthesiology. 2000 Jul;93(1):164-72
10861160
Eur J Pharmacol. 1996 Apr 22;301(1-3):41-8
8773445
Indian J Physiol Pharmacol. 1996 Jan;40(1):41-6
8864770
Curr Opin Neurobiol. 2002 Apr;12 (2):195-204
12015237
Pain. 1992 Jun;49(3):383-91
1357619
Pharmacol Biochem Behav. 1986 May;24(5):1441-4
3088610
Brain Res. 1983 Aug 29;273(2):245-52
6616237
Br J Pharmacol. 1995 Jul;115(6):981-6
7582530
J Neuroendocrinol. 1999 Jul;11(7):529-39
10444310
Neuroreport. 2000 May 15;11(7):1417-20
10841349
Brain Res. 1998 Dec 14;814(1-2):194-8
9838110
Prog Neuropsychopharmacol Biol Psychiatry. 2001 Jan;25(1):91-140
11263761
Eur J Pharmacol. 1999 Jun 30;375(1-3):261-76
10443582
J Chem Neuroanat. 1997 Jul;13(2):115-39
9285356