title: Transglutaminase 2 expression in the salivary myoepithelial cells of mouse embryo.
abstract: Earlier a strong transient expression of transglutaminase 2 (TGase 2) localized at the anchoring sites of muscle bundles in human embryo was observed. In this study, we report a similar transient expression of the TGase 2 in the salivary myoepithelial cells of mouse embryo by immunohistochemistry, RNA in situ hybridisation, and RT-PCR. From 35 submandibular glands of mouse embryos and postnatal mice, a consistent expression of TGase 2 in the myoepithelial cells via a stage-specific manner was identified by mono-clonal antibody to TGase 2 immunostaining. A similar expression pattern of TGase 2 in the myoepithelial cells was also observed by RNA in situ hybridisation analysis. The expression of TGase 2 in the salivary epithelium and mesenchyme during the prenatal 14.5-15.5 days was found minimally diffusely spread and became intensely focalised in the myoepithelial cells of salivary acini and ducts during the prenatal 16.5-18.5 days but thereafter gradually decreased until postnatal 7 days and remained weak in postnatal 3 weeks. Such transient rise and fall expressions of TGase 2 were also found with the sequential amount of RT-PCR products during the same period. The alpha-smooth muscle actin (alpha-SMA) as a positive control in the myoepithelial cells of mouse submandibular glands was consistently expressed during the prenatal and postnatal period. These results of transient expression of TGase 2 in the myoepithelial cells coincided with the formation of the dendritic basket structure in the periphery of acini and ducts, suggest a possible catalytic role of transglutaminase in a newly formed cellular matrixes during the cytodifferentiating stage of mouse prenatal and neonatal submandibular glands.
authors.affiliation: Department of Oral Pathology, College of Dentistry, Kangnung National University, Chibyondong, Gangneung, Gangwondo 210-702, Korea.
authors.forename: Suk Keun
authors.initials: SK
authors.lastname: Lee
authors.forename: Yeon Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Lee
authors.forename: Sang Shin
authors.initials: SS
authors.lastname: Lee
authors.forename: In Sun
authors.initials: IS
authors.lastname: Song
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Park
authors.forename: Je Geun
authors.initials: JG
authors.lastname: Chi
authors.forename: Soo Il
authors.initials: SI
authors.lastname: Chung
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004847:Epithelial Cells / Q000201:enzymology; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D008648:Mesoderm / Q000201:enzymology*; D051379:Mice; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000196:embryology / Q000201:enzymology*; D011503:Transglutaminases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins