Protective effects of FBD--an experimental Chinese traditional medicinal formula on memory dysfunction in mice induced by cerebral ischemia-reperfusion.
With regards to the applications of three Chinese herbs poria, rhizoma atractylodis macrocephalae, and radix angelicae sinensis to vascular dementia (VD), the work was performed to assess the nootropic action and explore neuroprotective mechanisms of three herbs combinations (FBD) on mice injured by cerebral repetitive ischemia-reperfusion (IR). Aqueous extracts from FBD (115-460 mg/kg) administered p.o. significantly improved cognitive function through elongating latency and reducing number of errors in step-through test. Aqueous extracts from FBD inhibited lipid peroxidation (LPO), elevated activity in (Na+)-(K+)-ATPase and (Ca2+)-ATPase, reduced the production of nitric oxide (NO) in cortical tissue after IR, and artificial cerebrospinal fluid (ACSF) containing aqueous extracts from FBD (ACSF-FBD) (0.01-10 mg/L) protected also primary cortical cortex neurons (PCCN) from hypoxic and excitotoxic insult induced by sodium dithionite (1 mM) and monosodium glutamate (MSG) (0.5 mM) in vitro. Multiple anti-IR properties contributed probably FBD to ameliorate cognitive dysfunction shown in this murine model for VD.
Lin
Zhihong
Z
Department of Chinese Medicinal Prescription, China Pharmaceutical University, Nanjing 210038, PR China.
Yan
Yongqing
Y
Zhu
Danni
D
Yu
Boyang
B
Wang
Qiujuan
Q
D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000188:drug therapy*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods; D008569:Memory Disorders / Q000188:drug therapy* / Q000209:etiology; D051379:Mice; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D018514:Plant Structures; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy*
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents