title: Capillary electrophoresis direct enantioseparation of aromatic amino acids based on mixed chelate-inclusion complexation of aminoethylamino-beta-cyclodextrin.
abstract: 6(A)-(2-Aminoethylamino)-6(A)-deoxy-beta-cyclodextrin (CDen) was synthesized and formed a binary complex with Cu(II) which was shown to be an effective chiral selector for separation of underivatized amino acid enantiomers in capillary electrophoresis (CE). Moreover, the chiral resolution was greatly enhanced by the presence of polyethyl glycol (PEG) and tert-butyl alcohol in the running buffer. The optimum experimental conditions were 20 mmol/L CDen, 20 mmol/L CuSO(4).5H(2)O, 5.0 mg/mL PEG20000 and 1.0% v/v tert-butyl alcohol, pH 5.80. With the proposed method, the four selected aromatic chiral amino acid pairs were separated in less than 15 min.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Bidong
authors.initials: B
authors.lastname: Wu
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Xie
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Liuhong
authors.initials: L
authors.lastname: Yun
mesh_terms: D024322:Amino Acids, Aromatic / Q000302:isolation & purification*; D002021:Buffers; D003300:Copper / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*; D020002:tert-Butyl Alcohol
chemical_list: C502270:6(A)-(2-aminoethylamino)-6(A)-deoxy-beta-cyclodextrin; D024322:Amino Acids, Aromatic; D002021:Buffers; D047392:beta-Cyclodextrins; D011092:Polyethylene Glycols; D003300:Copper; D020002:tert-Butyl Alcohol
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