Synergistic activity of Sef and Sprouty proteins in regulating the expression of Gbx2 in the mid-hindbrain region.
Sef and Sprouty proteins function as feedback antagonists of fibroblast growth factor (Fgf) signaling in zebrafish embryos. To study the role of Sef in mice, we generated Sef homozygous mutant animals. These animals are viable and show normal expression of mid-hindbrain genes at embryonic days 8.5 and 9.5. To investigate the possibility of functional synergism between Sef and Sprouty proteins, we electroporated Sprouty2(Y55A), which functions in a dominant-negative manner in tissue culture cells into the mid-hindbrain region of wildtype and Sef mutant embryos. The expression pattern of Gbx2, a downstream target of Fgf signaling, was expanded or shifted in electroporated embryos, and this effect was significantly enhanced in the Sef mutant background. Altogether, our results demonstrate that Sef and Sproutys function synergistically to regulate Gbx2 expression in the anterior hindbrain.
Lin
Wei
W
Medical Research Council, National Institute for Medical Research, London, UK.
Jing
Naihe
N
Basson
M Albert
MA
Dierich
Andrée
A
Licht
Jonathan
J
Ang
Siew-Lan
SL
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001933:Brain Stem / Q000196:embryology / Q000378:metabolism*; D003001:Cloning, Molecular; D018274:Electroporation; D046149:Embryo Culture Techniques; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D029961:Zebrafish Proteins
D048868:Adaptor Proteins, Signal Transducing; C513182:Gbx2 protein, mouse; D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; C469338:Sef protein, mouse; D029961:Zebrafish Proteins; C513181:gbx2 protein, zebrafish; D005346:Fibroblast Growth Factors; D017346:Protein Serine-Threonine Kinases; C118389:Spry2 protein, mouse