Maternal transmission ratio distortion at the mouse Om locus results from meiotic drive at the second meiotic division.
We have observed maternal transmission ratio distortion (TRD) in favor of DDK alleles at the Ovum mutant (Om) locus on mouse chromosome 11 among the offspring of (C57BL/6 x DDK) F(1) females and C57BL/6 males. Although significant lethality occurs in this backcross ( approximately 50%), differences in the level of TRD found in recombinant vs. nonrecombinant chromosomes among offspring argue that TRD is due to nonrandom segregation of chromatids at the second meiotic division, i.e., true meiotic drive. We tested this hypothesis directly, by determining the centromere and Om genotypes of individual chromatids in zygote stage embryos. We found similar levels of TRD in favor of DDK alleles at Om in the female pronucleus and TRD in favor of C57BL/6 alleles at Om in the second polar body. In those embryos for which complete dyads have been reconstructed, TRD was present only in those inheriting heteromorphic dyads. These results demonstrate that meiotic drive occurs at MII and that preferential death of one genotypic class of embryo does not play a large role in the TRD.
Wu
Guangming
G
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Hao
Lanping
L
Han
Zhiming
Z
Gao
Shaorong
S
Latham
Keith E
KE
de Villena
Fernando Pardo-Manuel
FP
Sapienza
Carmen
C
D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D008297:Male; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000166:cytology*; D015053:Zygote / Q000166:cytology*
Mamm Genome. 1999 May;10(5):492-7
10337624
Mol Hum Reprod. 1997 Aug;3(8):693-8
9294853
Genetics. 2000 Jan;154(1):351-6
10628994
Genetics. 2000 May;155(1):283-9
10790402
Genetics. 2000 Oct;156(2):775-83
11014823
Mamm Genome. 2001 May;12(5):331-9
11331939
J Cell Sci. 2001 Jul;114(Pt 13):2417-26
11559750
Genetics. 2001 Nov;159(3):1179-89
11729161
Genetics. 2002 Mar;160(3):1123-31
11901128
Genetics. 2002 Aug;161(4):1651-9
12196408
J Reprod Fertil. 1973 May;33(2):283-91
4736011
J Reprod Fertil. 1974 Nov;41(1):85-96
4431024
J Reprod Fertil. 1986 Mar;76(2):779-81
3754584
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6883-6
3462735
J Reprod Fertil. 1989 Jul;86(2):679-88
2760894
Genet Res. 1990 Apr;55(2):97-100
2164490
Dev Suppl. 1990;:81-7
2090434
Genetics. 1992 Sep;132(1):241-6
1398057
J Med Genet. 1993 Nov;30(11):903-9
8301643
Genetics. 1996 Apr;142(4):1299-304
8846906
Mamm Genome. 1996 Feb;7(2):114-6
8835527
Mamm Genome. 1997 Sep;8(9):642-6
9271664
Genetics. 2000 Jan;154(1):333-42
10628992