Isolation of Delta9-THCA-A from hemp and analytical aspects concerning the determination of Delta9-THC in cannabis products.
A simple procedure based on a common silica gel column chromatography for the isolation of Delta9-tetrahydrocannabinolic acid A (Delta9-THCA-A) from hemp in a multi-milligram scale is presented. Further, the decarboxylation reaction of Delta9-THCA-A to the toxicologically active Delta9-tetrahydrocannabinol (Delta9-THC) at different analytical and under-smoking conditions is investigated. Maximal conversion in an optimised analytical equipment yields about 70% Delta9-THC. In the simulation of the smoking process, only about 30 % of the spiked substance could be recovered as Delta9-THC.
Dussy
Franz E
FE
Institute of Legal Medicine, Pestalozzistrasse 22, CH-4056 Basel, Switzerland. franz.dussy@bs.ch
Hamberg
Cornelia
C
Luginbühl
Marco
M
Schwerzmann
Thomas
T
Briellmann
Thomas A
TA