title: Separation of mutation avoidance and antirecombination functions in an Escherichia coli mutS mutant.
abstract: DNA mismatch repair in Escherichia coli has been shown to be involved in two distinct processes: mutation avoidance, which removes potential mutations arising as replication errors, and antirecombination which prevents recombination between related, but not identical (homeologous), DNA sequences. We show that cells with the mutSDelta800 mutation (which removes the C-terminal 53 amino acids of MutS) on a multicopy plasmid are proficient for mutation avoidance. In interspecies genetic crosses, however, recipients with the mutSDelta800 mutation show increased recombination by up to 280-fold relative to mutS+. The MutSDelta800 protein binds to O6-methylguanine mismatches but not to intrastrand platinated GG cross-links, explaining why dam bacteria with the mutSDelta800 mutation are resistant to cisplatin, but not MNNG, toxicity. The results indicate that the C-terminal end of MutS is necessary for antirecombination and cisplatin sensitization, but less significant for mutation avoidance. The inability of MutSDelta800 to form tetramers may indicate that these are the active form of MutS.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School 364 Plantation Street, Worcester, MA 01605, USA.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Calmann
authors.forename: Anetta
authors.initials: A
authors.lastname: Nowosielska
authors.forename: M G
authors.initials: MG
authors.lastname: Marinus
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020137:Base Pair Mismatch; D002945:Cisplatin / Q000633:toxicity; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004265:DNA Helicases / Q000378:metabolism; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D051719:MutS DNA Mismatch-Binding Protein; D009154:Mutation; D009841:Oligonucleotides / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009841:Oligonucleotides; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli; D004265:DNA Helicases; D002945:Cisplatin
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