Correlation between the histological grade of chondrosarcoma and the expression of MMPs, ADAMTSs and TIMPs.
BACKGROUND
The malignant degree of human chondrosarcoma can be difficult to determine using only histological findings. We, therefore, assessed the expression of matrix metalloproteinases (MMPs), a disintegrin and metalloproteinase (ADAM) with thrombospondin motifs (ADAMTSs) and tissue inhibitor of metalloproteinases (TIMPs) in chondrosarcoma and ascertained the relationships to histological degree of malignancy and prognosis.


METHODS
In 28 chondrosarcoma cases, immunostaining was performed using antibodies against MMP 2, 3, 7, 9, 13, ADAMTS 4, 5 and TIMP 1, 2, 3.


RESULTS
The chondrosarcoma were classified into groups of 7, 15 and 6 cases based on histologically malignant grade I, II and III, respectively. All target proteins were expressed in chondrosarcoma. Positive correlations (p < 0.05) existed between immunostaining scores and histological grades for all proteins except MMP 9, with strong correlations (p < 0.01) for MMPs 2, 3 and 13, both ADAMTSs and all 3 TIMPs. No correlation existed between prognosis and immunostaining scores.


CONCLUSIONS
These target proteins could, thus, indicate the degree of malignancy in human chondrosarcoma.
Sugita
Hideyuki
H
Department of Orthopaedic Surgery, Nihon University School of Medicine, Tokyo, Japan. joe_sugisaku@tiara.ocn.ne.jp
Osaka
Shunzo
S
Toriyama
Masato
M
Osaka
Eiji
E
Yoshida
Yukihiro
Y
Ryu
Junnosuke
J
Sano
Makoto
M
Sugitani
Masahiko
M
Nemoto
Norimichi
N
D051722:ADAM Proteins; D000071121:ADAMTS4 Protein; D000071116:ADAMTS5 Protein; D001859:Bone Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D002813:Chondrosarcoma / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D011348:Procollagen N-Endopeptidase; D011379:Prognosis; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis*
D007527:Isoenzymes; D019714:Tissue Inhibitor of Metalloproteinases; D051722:ADAM Proteins; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human; D020782:Matrix Metalloproteinases; D008666:Metalloendopeptidases; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human