title: Presence of Bacillus cereus in street foods in Gaborone, Botswana.
abstract: The purpose of this study was to evaluate the microbiological safety and quality of street foods sold in Gaborone, Botswana. A total of 148 point-of-sale composite street food samples were bought and analyzed between June 2001 and May 2002. The analysis focused on the level of contamination of various street foods with Bacillus cereus. The B. cereus (vegetative and spores), total spore, and total viable counts were determined on all the samples. Also B. cereus isolates from 444 individual point-of-sale food samples were characterized with respect to their biochemical profiles and enterotoxigenic properties. The B. cereus contamination rate for point-of-sale foods was 65%. The B. cereus counts ranged from not detectable to levels as high as 9.1 log CFU/g. Despite the high rate of contamination of some samples, generally, most samples had B. cereus counts of less than 4 log CFU/g; hence, they were of acceptable microbiological quality. Bacillus diarrheal enterotoxin was detected from 52 isolates from individual portions of meals using the B. cereus enterotoxin reversed passive latex agglutination kit. Results of the assay revealed that 59.6% of the B. cereus isolates were enterotoxigenic. Most of the enterotoxigenic isolates were obtained from vegetable samples.
authors.affiliation: Department of Biological Sciences, University of Botswana, Gaborone, Botswana.
authors.forename: Gervas Y
authors.initials: GY
authors.lastname: Murindamombe
authors.forename: Ernest K
authors.initials: EK
authors.lastname: Collison
authors.forename: Sisai F
authors.initials: SF
authors.lastname: Mpuchane
authors.forename: Berhanu A
authors.initials: BA
authors.lastname: Gashe
mesh_terms: D001409:Bacillus cereus / Q000302:isolation & purification* / Q000378:metabolism; D001902:Botswana; D015169:Colony Count, Microbial; D003132:Commerce; D003257:Consumer Product Safety; D004768:Enterotoxins / Q000302:isolation & purification; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling / Q000379:methods / Q000592:standards; D005516:Food Microbiology; D006801:Humans; D007841:Latex Fixation Tests; D013171:Spores, Bacterial / Q000302:isolation & purification; D014675:Vegetables / Q000382:microbiology*
chemical_list: D004768:Enterotoxins