title: Surface motion upregulates superficial zone protein and hyaluronan production in chondrocyte-seeded three-dimensional scaffolds.
abstract: A cartilage engineering bioreactor has been developed that provides joint-specific kinematics. This study investigated the effect of articular motion on the gene expression of superficial zone protein (SZP) and hyaluronan synthases (HASs) and on the release of SZP and hyaluronan of chondrocytes seeded onto biodegradable scaffolds. Cylindrical (8 x 4 mm) porous polyurethane scaffolds were seeded with bovine articular chondrocytes and subjected to static or dynamic compression, with and without articulation against a ceramic hip ball. After loading, the mRNA expression of SZP and HASs was analyzed, and SZP immunoreactivity and hyaluronan concentration of conditioned media were determined. Surface motion significantly upregulated the mRNA expression of SZP and HASs. Axial compression alone had no effect on SZP and increased HAS mRNA only at high strain amplitude. SZP was immunodetected only in the media of constructs exposed to surface motion. The release of hyaluronan into the culture medium was significantly enhanced by surface motion. These results indicate that specific stimuli that mimic the kinematics of natural joints, such as articular motion, may promote the development of a functional articular surface-synovial interface.
authors.affiliation: Biochemistry and Cell Biology, AO Research Institute, Davos Platz, Switzerland.
authors.forename: Sibylle
authors.initials: S
authors.lastname: Grad
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Lee
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Gorna
authors.forename: Sylwester
authors.initials: S
authors.lastname: Gogolewski
authors.forename: Markus A
authors.initials: MA
authors.lastname: Wimmer
authors.forename: Mauro
authors.initials: M
authors.lastname: Alini
mesh_terms: D000818:Animals; D019149:Bioreactors; D002358:Cartilage, Articular / Q000166:cytology; D002417:Cattle; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D017077:Culture Media, Conditioned; D005786:Gene Expression Regulation / Q000187:drug effects; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007596:Joints / Q000166:cytology; D009038:Motion; D011140:Polyurethanes / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013314:Stress, Mechanical; D015854:Up-Regulation
chemical_list: D017077:Culture Media, Conditioned; D011140:Polyurethanes; D011506:Proteins; D012333:RNA, Messenger; D006820:Hyaluronic Acid