title: Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances paraquat-induced photooxidative stress and nitric oxide-induced cell death.
abstract: The production and characterization of Arabidopsis plants containing a transgene in which the Arabidopsis tAPX is inserted in antisense orientation, is described. tAPX activity in these transgenic tAPX plants is around 50% of control level. The tAPX antisense plants are phenotypically indistinguishable from control plants under normal growth conditions; they show, however, enhanced sensitivity to the O2- -generating herbicide, Paraquat. Interestingly, the tAPX antisense plants show enhanced symptoms of damage when cell death is triggered through treatment with the nitric oxide-donor, SNP. These results are in accordance with the ones recently obtained with transgenic plants overexpressing tAPX; altogether, they suggest that tAPX, besides the known ROS scavenging role, is also involved in the fine changes of H2O2 concentration during signaling events.
authors.affiliation: Dipartimento di Biologia, Sezione di Fisiologia e Biochimica delle Piante, Università degli Studi di Milano, via Celoria 26, 20133, Italy.
authors.forename: Delia
authors.initials: D
authors.lastname: Tarantino
authors.forename: Candida
authors.initials: C
authors.lastname: Vannini
authors.forename: Marcella
authors.initials: M
authors.lastname: Bracale
authors.forename: Manuela
authors.initials: M
authors.lastname: Campa
authors.forename: Carlo
authors.initials: C
authors.lastname: Soave
authors.forename: Irene
authors.initials: I
authors.lastname: Murgia
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins; D060387:Ascorbate Peroxidases; D016923:Cell Death / Q000502:physiology; D016373:DNA, Antisense; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006540:Herbicides / Q000494:pharmacology; D008027:Light; D009569:Nitric Oxide / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology*; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D030821:Plants, Genetically Modified; D060431:Thylakoid Membrane Proteins; D020524:Thylakoids / Q000201:enzymology
chemical_list: D029681:Arabidopsis Proteins; D016373:DNA, Antisense; D006540:Herbicides; D060431:Thylakoid Membrane Proteins; D009569:Nitric Oxide; D010544:Peroxidases; C555298:APXT protein, Arabidopsis; D060387:Ascorbate Peroxidases; D010269:Paraquat
references.citation: Plant Physiol. 1949 Jan;24(1):1-15
references.pmid: 16654194
references.citation: Photosynth Res. 2001;68(2):175-80
references.pmid: 16228340
references.citation: Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1419-31
references.pmid: 11127996
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:601-639
references.pmid: 15012221
references.citation: Plant Cell Physiol. 1996 Jan;37(1):49-59
references.pmid: 8720924
references.citation: Plant J. 2002 Nov;32(3):329-42
references.pmid: 12410811
references.citation: Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14165-70
references.pmid: 10570216
references.citation: Plant Mol Biol. 1999 Mar;39(5):1025-35
references.pmid: 10344207
references.citation: Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13454-9
references.pmid: 11606758
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant Physiol. 1993 Sep;103(1):165-170
references.pmid: 12231923
references.citation: Plant Physiol. 2000 May;123(1):223-34
references.pmid: 10806239
references.citation: J Exp Bot. 2002 May;53(372):1305-19
references.pmid: 11997377
references.citation: Trends Plant Sci. 2002 Sep;7(9):405-10
references.pmid: 12234732
references.citation: Plant Physiol. 1995 Apr;107(4):1049-1054
references.pmid: 12228418
references.citation: J Exp Bot. 2001 Dec;52(365):2345-54
references.pmid: 11709584
references.citation: Nature. 1998 Aug 6;394(6693):585-8
references.pmid: 9707120
references.citation: Biochim Biophys Acta. 2001 May 3;1526(2):168-74
references.pmid: 11325538
references.citation: Plant Physiol. 2000 Jul;123(3):895-904
references.pmid: 10889238
references.citation: Planta. 2003 Sep;217(5):709-16
references.pmid: 12728319
references.citation: Plant Physiol. 1997 May;114(1):275-284
references.pmid: 12223704
references.citation: Trends Plant Sci. 2001 Jun;6(6):262-7
references.pmid: 11378468
references.citation: Planta. 1998 Jan;204(1):120-6
references.pmid: 9443387
references.citation: Plant Physiol. 1997 Jun;114(2):529-537
references.pmid: 12223726
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:249-279
references.pmid: 15012235
references.citation: Free Radic Biol Med. 1997;23(3):473-9
references.pmid: 9214585
references.citation: Plant Physiol. 2002 Oct;130(2):698-708
references.pmid: 12376637
references.citation: Plant J. 2004 Jun;38(6):940-53
references.pmid: 15165186
references.citation: Plant Cell Physiol. 1999 Jul;40(7):725-32
references.pmid: 10501032
references.citation: Plant Cell. 1998 Mar;10 (3):461-73
references.pmid: 9501118
references.citation: Biochem J. 1997 Sep 1;326 ( Pt 2):305-10
references.pmid: 9291097
references.citation: J Plant Physiol. 2004 Jul;161(7):777-83
references.pmid: 15310066
references.citation: Gene. 2001 Jul 25;273(1):23-7
references.pmid: 11483357
references.citation: Plant Physiol. 2002 Jun;129(2):838-53
references.pmid: 12068123
references.citation: Plant J. 2002 Dec;32(6):915-25
references.pmid: 12492834
references.citation: Nature. 2000 Aug 17;406(6797):731-4
references.pmid: 10963598