Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances paraquat-induced photooxidative stress and nitric oxide-induced cell death.
The production and characterization of Arabidopsis plants containing a transgene in which the Arabidopsis tAPX is inserted in antisense orientation, is described. tAPX activity in these transgenic tAPX plants is around 50% of control level. The tAPX antisense plants are phenotypically indistinguishable from control plants under normal growth conditions; they show, however, enhanced sensitivity to the O2- -generating herbicide, Paraquat. Interestingly, the tAPX antisense plants show enhanced symptoms of damage when cell death is triggered through treatment with the nitric oxide-donor, SNP. These results are in accordance with the ones recently obtained with transgenic plants overexpressing tAPX; altogether, they suggest that tAPX, besides the known ROS scavenging role, is also involved in the fine changes of H2O2 concentration during signaling events.
Tarantino
Delia
D
Dipartimento di Biologia, Sezione di Fisiologia e Biochimica delle Piante, Università degli Studi di Milano, via Celoria 26, 20133, Italy.
Vannini
Candida
C
Bracale
Marcella
M
Campa
Manuela
M
Soave
Carlo
C
Murgia
Irene
I
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins; D060387:Ascorbate Peroxidases; D016923:Cell Death / Q000502:physiology; D016373:DNA, Antisense; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006540:Herbicides / Q000494:pharmacology; D008027:Light; D009569:Nitric Oxide / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology*; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D030821:Plants, Genetically Modified; D060431:Thylakoid Membrane Proteins; D020524:Thylakoids / Q000201:enzymology
D029681:Arabidopsis Proteins; D016373:DNA, Antisense; D006540:Herbicides; D060431:Thylakoid Membrane Proteins; D009569:Nitric Oxide; D010544:Peroxidases; C555298:APXT protein, Arabidopsis; D060387:Ascorbate Peroxidases; D010269:Paraquat
Plant Physiol. 1949 Jan;24(1):1-15
16654194
Photosynth Res. 2001;68(2):175-80
16228340
Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1419-31
11127996
Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:601-639
15012221
Plant Cell Physiol. 1996 Jan;37(1):49-59
8720924
Plant J. 2002 Nov;32(3):329-42
12410811
Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14165-70
10570216
Plant Mol Biol. 1999 Mar;39(5):1025-35
10344207
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13454-9
11606758
Plant J. 1998 Dec;16(6):735-43
10069079
Plant Physiol. 1993 Sep;103(1):165-170
12231923
Plant Physiol. 2000 May;123(1):223-34
10806239
J Exp Bot. 2002 May;53(372):1305-19
11997377
Trends Plant Sci. 2002 Sep;7(9):405-10
12234732
Plant Physiol. 1995 Apr;107(4):1049-1054
12228418
J Exp Bot. 2001 Dec;52(365):2345-54
11709584
Nature. 1998 Aug 6;394(6693):585-8
9707120
Biochim Biophys Acta. 2001 May 3;1526(2):168-74
11325538
Plant Physiol. 2000 Jul;123(3):895-904
10889238
Planta. 2003 Sep;217(5):709-16
12728319
Plant Physiol. 1997 May;114(1):275-284
12223704
Trends Plant Sci. 2001 Jun;6(6):262-7
11378468
Planta. 1998 Jan;204(1):120-6
9443387
Plant Physiol. 1997 Jun;114(2):529-537
12223726
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:249-279
15012235
Free Radic Biol Med. 1997;23(3):473-9
9214585
Plant Physiol. 2002 Oct;130(2):698-708
12376637
Plant J. 2004 Jun;38(6):940-53
15165186
Plant Cell Physiol. 1999 Jul;40(7):725-32
10501032
Plant Cell. 1998 Mar;10 (3):461-73
9501118
Biochem J. 1997 Sep 1;326 ( Pt 2):305-10
9291097
J Plant Physiol. 2004 Jul;161(7):777-83
15310066
Gene. 2001 Jul 25;273(1):23-7
11483357
Plant Physiol. 2002 Jun;129(2):838-53
12068123
Plant J. 2002 Dec;32(6):915-25
12492834
Nature. 2000 Aug 17;406(6797):731-4
10963598