[Caco-2 cell monolayers and it's application in toxicological research].
Caco-2 cells derived from a human colon adenocarcinoma, spontaneously differentiate after reaching confluence in culture, exhibiting several morphological and functional characteristics of mature enterocytes. Caco-2 cell monolayers represents a well-established model for the study of intestinal transport of nutrients and xenobiotics, and is wildly used in pharmacology and toxicology. In this review the cell culture conditions, characteristics and functional parameters of model were introduced. The application of Caco-2 cell monolayers in studying mechanism, uptake, transport and gastrointestinal toxicity of xenobiotics were also explained.
Liu
Zhiwei
Z
School of Public Health, Fudan University, Shanghai 200032, China.
Chen
Bingheng
B
D001692:Biological Transport; D018938:Caco-2 Cells; D018929:Cell Culture Techniques; D002463:Cell Membrane Permeability; D006801:Humans; D014116:Toxicology