Effect of the rhizomes of Astilbe chinensis on UVB-induced inflammatory response.
The EtOH extract from the rhizomes of Astilbe chinensis (Saxifragaceae) exhibited potent antioxidant activity in preliminary screening. Since oxidative stress is known to be involved in the inflammatory response after UVB exposure, the ability of an A. chinensis extract to inhibit UVB-induced PGE2 and NO production was examined. UVB irradiation (35 mJ/cm2) increased PGE2 and NO production, which were significantly decreased by pre-administration of the extract in a dose-dependent manner. The A. chinensis extract also preserved the cellular antioxidant capacity after UVB irradiation, which was determined by the GSH content. UVB irradiation enhanced the formation of ROS in the keratinocytes, which was determined using DCFH-DA, a redox sensitive dye. The levels of intracellular ROS were also significantly reduced by pre-treatment of the extract in a dose-dependent manner measured 9 h after UVB irradiation. The results suggest that the A. chinensis extract may have a protective effect on the UVB-injured keratinocytes by inhibiting PGE2 and NO production, possibly through the inhibition of intracellular ROS accumulation.
Na
Minkyun
M
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Min
Byung Sun
BS
An
Ren Bo
RB
Jin
Wenyi
W
Kim
Young Ho
YH
Song
Kyung Sik
KS
Seong
Yeon Hee
YH
Bae
KiHwan
K
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D027343:Rhizome; D031995:Saxifragaceae; D014466:Ultraviolet Rays
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D005978:Glutathione; D015232:Dinoprostone