beta-Catenin expression during vascular development and degeneration of avian mesonephros.
beta-Catenin is a structural component of adherens junctions, a regulator of the Wnt signalling pathway and a transcriptional co-activator with a key role in vascular patterning. The avian mesonephros is a transitory embryonic kidney that is used in the study of vascular development and degeneration. Here we examine beta-catenin expression in this model during vascular development and degeneration. Quail embryos with developing or degenerating mesonephros were studied, on day 6 (30HH) or day 11 of incubation (40HH), respectively. QH1 whole mounts of developing mesonephros revealed numerous angioblast-like cells situated in the paramesonephric duct that seem to invade the mesonephros. Although these cells did not express beta-catenin, the surrounding periductal mesenchymal cells translocated high levels of beta-catenin into the nucleus. In contrast, degenerating mesonephros were devoid of angioblast-like cells and beta-catenin was lower than in the developing mesonephros. beta-Catenin was significantly reduced in the glomerular capillary tuffs, indicating that it was particularly down-regulated in the vascular system. No sex-related differences in beta-catenin expression were observed in degenerating mesonephros. Furthermore, two special populations of glomerular and peritubular endothelial cells were observed in degenerating mesonephros: one translocating beta-catenin into the nucleus and the other in apoptosis that did not translocate it. In conclusion, our results indicate that the paramesonephric duct is a potential new vasculogenetic pathway, and suggest that beta-catenin plays a role in the fate of mesonephric endothelial cells.
Nacher
Víctor
V
Department of Animal Health and Anatomy and Center for Animal Biotechnology and Gene Therapy (CBATEG), Autonomous University of Barcelona, Spain.
Carretero
Ana
A
Navarro
Marc
M
Armengol
Clara
C
Llombart
Cristina
C
Blasi
Juan
J
Ruberte
Jesús
J
D000818:Animals; D017209:Apoptosis; D001692:Biological Transport; D001808:Blood Vessels; D015153:Blotting, Western / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008650:Mesonephros / Q000098:blood supply / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D011784:Quail / Q000196:embryology*; D015534:Trans-Activators / Q000032:analysis* / Q000378:metabolism; D051176:beta Catenin
D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin
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