Hemopoietic chimerism following stem cell transplantation.
Hematopoietic chimerism is a measure of the number of donor and recipient cells in the host following stem cell transplantation (SCT). The type of conditioning therapy prior to SCT has a major impact on the chimeric status in the recipient. Different techniques of measurement have varying sensitivities. The use of polymerase chain reaction (PCR) of short tandem repeats (STR) using fluorescent amplification permits quantification using Genescan analysis. When SCT is used for malignant haematological disorders, measurement of chimeric status may indicate early relapse and in aplastic anemia graft rejection. Reduced intensity or T-cell depletion is associated with mixed haemopoietic chimerism. SCT for benign haematological disorders does not require complete donor chimerism for a successful outcome.
McCann
Shaun R
SR
Department of Hematology, St James's Hospital/University of Dublin, Trinity College, Durkan Building, Trinity Center, Dublin 8, Ireland. smccann@stjames.ie
Crampe
Mireille
M
Molloy
Karen
K
Lawler
Mark
M
D000741:Anemia, Aplastic / Q000628:therapy; D005260:Female; D006402:Hematologic Diseases / Q000628:therapy; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000628:therapy; D008297:Male; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000379:methods*; D020080:Tandem Repeat Sequences; D018183:Transplantation Chimera; D014184:Transplantation, Homologous; D016896:Treatment Outcome