Serological screening for MHC (B)-polymorphism in indigenous chickens.
As part of a series of studies to characterize innate and specific immune responses of indigenous chicken lines, birds from Bolivia and India were screened serologically for MHC class IV (BG) polymorphism by direct haemagglutination using haplotype-specific antisera (B2, B4, B12, B13, B14, B15, B19, B21). The sample consisted of 95 Bolivian indigenous chickens and 119 hens from the four most common North Indian 'back-yard' chicken lines: Yellow Aseel (AP), Kadaknath (KN), frizzled typed (Ff-) and naked neck (NN). Of all chickens tested, the majority were haplotyped as B2, B15, B19 and B21. Of the Bolivian chickens, 89.5% could be haplotyped: 54.9% were homozygous (including 43.3% B15), and 34.6% were heterozygous (including 15.7% B15). B2-like haplotypes were not found among the Bolivian hens, and only 3.2% of these birds showed homozygous B21-like proteins. Of the Indian hens, MHC (BG)-like proteins could be detected in 60.0% of the AP birds, 6.7% of the KN birds; 40.0% of the Ff- birds; and 10.3% of the NN birds. In these lines, a total of 40.1% (AP), 6.7% (KN), 30.1% (Ff-) and 10.3% (NN) were homozygous for the B-haplotype. Only in the AP line (19.9%), and the Ff- line (9.9%) were heterozygous B-haplotypes types found. The B2 haplotype was found in all Indian chicken lines. Most Indian birds have completely unknown haplotypes, indicating a potentially interesting genetic pool. Subgrouping the Bolivian and Indian indigenous hens into monomorphic BG populations revealed individual differences based on the B-types.
Baelmans
R
R
Institute of Tropical Medicine, Department of Animal Health, Antwerp, Belgium.
Parmentier
H K
HK
Nieuwland
M G B
MG
Dorny
P
P
Demey
F
F
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D005260:Female; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000276:immunology*
D006649:Histocompatibility Antigens
Poult Sci. 1993 Mar;72(3):391-402
8385327
Vet Immunol Immunopathol. 1991 Nov;30(1):121-7
1781152
Poult Sci. 1998 Aug;77(8):1111-8
9706074
Avian Dis. 1993 Jan-Mar;37(1):53-9
8383961
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4397-401
7910407
Philos Trans R Soc Lond B Biol Sci. 2000 Aug 29;355(1400):1077-84
11186309
J Hered. 1997 Nov-Dec;88(6):504-12
9419890
Scand J Immunol. 1981 Dec;14(6):607-16
7043723
Anim Genet. 2001 Jun;32(3):123-31
11493260
Immunogenetics. 1996;45(1):52-8
8881037
Anim Genet. 1991;22(4):323-32
1952282
Poult Sci. 1987 May;66(5):790-4
3306643
Anim Genet. 2000 Aug;31(4):252-61
11086534
Avian Dis. 1994 Jan-Mar;38(1):65-71
8002902
Vet Immunol Immunopathol. 2004 Jul;100(1-2):25-32
15182993
Immunogenetics. 1993;37(6):408-14
8436415
Rev Sci Tech. 1998 Apr;17(1):128-42
9638806
Crit Rev Immunol. 1992;12(1-2):47-79
1358107
Crit Rev Immunol. 1995;15(1):31-75
8519422
Immunogenetics. 1992;36(4):270-3
1353480
Avian Dis. 1992 Apr-Jun;36(2):378-85
1320867
Poult Sci. 1994 Apr;73(4):481-7
8202426
Vet Immunol Immunopathol. 1993 Dec;39(4):407-19
8116218
Immunogenetics. 1999 Mar;49(3):215-24
9914335
Immunogenetics. 1996;43(5):277-88
9110931
Immunogenetics. 1982;16(6):513-32
6763913
EMBO J. 1988 Sep;7(9):2775-85
3141149
Trop Anim Health Prod. 2004 Nov;36(8):731-42
15643809
Avian Dis. 1968 Feb;12(1):9-28
5643702
Trop Anim Health Prod. 2005 Apr;37(3):173-86
15747855
Nature. 1999 Oct 28;401(6756):923-5
10553909
Vet Immunol Immunopathol. 2002 Nov;90(1-2):91-100
12406658
Curr Top Microbiol Immunol. 1996;212:129-41
8934816