Expression of Cu/Zn SOD protein is suppressed in hsp 70.1 knockout mice.
Heat shock proteins (HSPs) are known to protect cells from oxidative stress and other types of injuries. We previously reported the neuroprotective effect of HSP70 following cerebral ischemia and reperfusion using hsp 70.1 knockout (KO) mice. However, the precise role of HSP70 in neuroprotection has not been established yet. The purpose of this study was to investigate the relationship between HSP70 and antioxidant enzymes using hsp 70.1 KO mice. The activities of both SOD-1 and SOD-2 were significantly decreased in hsp 70.1 KO mice than in the wild type (WT) littermates. SOD-1 protein level in the hsp 70.1 KO mice was lower than that of WT. We speculate that HSP70 might be involved in regulation of expression of SOD-1 at the level of transcription or by post-transcriptional modification.
Choi
Smi
S
College of Nursing, Seoul National University, Seoul 110-744, Korea.
Park
Kyung Ae
KA
Lee
Hee Joo
HJ
Park
Myoung Sook
MS
Lee
Joung Hee
JH
Park
Kyoung Chan
KC
Kim
Manho
M
Lee
Seung-Hoon
SH
Seo
Jeong-Sun
JS
Yoon
Byung-Woo
BW
D000818:Animals; D001921:Brain / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D015536:Down-Regulation; D018390:Gene Targeting; D005979:Glutathione Peroxidase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2
D002374:Catalase; D005979:Glutathione Peroxidase; D018840:HSP70 Heat-Shock Proteins; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2; C081884:heat-shock protein 70.1; C000606291:Sod1 protein, mouse