title: Protein identification using 2D-LC-MS/MS.
abstract: Multidimensional liquid chromatography techniques have been coupled to tandem mass spectrometry to provide a robust method to identify proteins in complex mixtures. Data acquisition is interfaced directly with search algorithms for identification through cross-correlation with databases. This review describes the most recent advances in methodologies for protein identification by mass spectrometry and describes the limitations of the application of the technologies.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Claire
authors.initials: C
authors.lastname: Delahunty
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
mesh_terms: D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D012984:Software
chemical_list: D010455:Peptides; D011506:Proteins; D020543:Proteome