title: Survival of Lactobacillus sakei during heating, drying and storage in the dried state when growth has occurred in the presence of sucrose or monosodium glutamate.
abstract: Spray-dried cells of Lactobacillus sakei CTC 494 survived ca. 60% longer in the spray dried state when cells were grown in the presence of 20 g sucrose l(-1) or 12.5 g monosodium glutamate l(-1). No significant differences were observed in viability during storage in the freeze dried state with the addition of these compounds to the growth medium, nor in survival during a heat treatment (55 degrees C). Both sucrose and glutamate in the growth medium suppressed intracellular accumulation of total amino acids and changed the overall pattern of the individual amino acids. Glutamate in the growth medium enhanced intracellular glutamate by ca. 38%.
authors.affiliation: Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal.
authors.forename: Vânia
authors.initials: V
authors.lastname: Ferreira
authors.forename: Vânia
authors.initials: V
authors.lastname: Soares
authors.forename: Cristina
authors.initials: C
authors.lastname: Santos
authors.forename: Joana
authors.initials: J
authors.lastname: Silva
authors.forename: Paul A
authors.initials: PA
authors.lastname: Gibbs
authors.forename: Paula
authors.initials: P
authors.lastname: Teixeira
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology*; D005612:Freeze Drying; D006358:Hot Temperature; D007778:Lactobacillus / Q000502:physiology*; D012970:Sodium Glutamate / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods*; D013395:Sucrose / Q000378:metabolism*
chemical_list: D013395:Sucrose; D012970:Sodium Glutamate