title: Increased apoptosis during morphogenesis of the lower cheek teeth in tabby/EDA mice.
abstract: In wild-type (WT) mice, epithelial apoptosis is involved in reducing the embryonic tooth number and the mesial delimitation of the first molar. We investigated whether apoptosis could also be involved in the reduction of tooth number and the determination of anomalous tooth boundaries in tabby (Ta)/EDA mice. Using serial histological sections and computer-aided 3D reconstructions, we investigated epithelial apoptosis in the lower cheek dentition at embryonic days 14.5-17.5. In comparison with WT mice, apoptosis was increased mainly mesially in Ta dental epithelium from day 15.5. This apoptosis showed a similar mesio-distal extent in all 5 morphotypes (Ia,b,c and IIa,b) of Ta dentition and eliminated the first cheek tooth in morphotypes IIa,b. Apoptosis did not appear to play any causal role in positioning inter-dental gaps. Analysis of the present data suggests that the increased apoptosis in Ta mice is a consequence of impaired tooth development caused by a defect in segmentation of dental epithelium.
authors.affiliation: Department of Teratology, Institute of Experimental Medicine, Academy of Sciences of the CR, Prague, Czech Republic.
authors.forename: T
authors.initials: T
authors.lastname: Boran
authors.forename: H
authors.initials: H
authors.lastname: Lesot
authors.forename: M
authors.initials: M
authors.lastname: Peterka
authors.forename: R
authors.initials: R
authors.lastname: Peterkova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002610:Cheek / Q000196:embryology; D004476:Ectodermal Dysplasia / Q000196:embryology* / Q000235:genetics; D053331:Ectodysplasins; D004658:Enamel Organ / Q000196:embryology; D004848:Epithelium / Q000196:embryology; D005865:Gestational Age; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008334:Mandible / Q000196:embryology; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D009024:Morphogenesis / Q000502:physiology*; D009805:Odontogenesis / Q000502:physiology*; D014083:Tooth Germ / Q000196:embryology*
chemical_list: D053331:Ectodysplasins; C506019:Eda protein, mouse; D008565:Membrane Proteins