Effect of bacoside A on membrane-bound ATPases in the brain of rats exposed to cigarette smoke.
Membrane-bound enzymes play a vital role in neuronal function through maintenance of membrane potential and impulse propagation. We have evaluated the harmful effects of chronic cigarette smoking on membrane-bound ATPases and the protective effect of Bacoside A in rat brain. Adult male albino rats were exposed to cigarette smoke for a period of 12 weeks and simultaneously administered with Bacoside A (the active principle isolated from Bacopa monniera) at a dosage of 10 mg/kg b.w/day, p.o. The levels of lipid peroxides as marker for evaluating the extent of membrane damage, the activities of Na+/K+-ATPase, Ca2+-ATPase and Mg2+-ATPase, and associated cations sodium (Na+), potassium (K+), calcium (Ca2+), and magnesium (Mg2+) were investigated in the brain. Neuronal membrane damage was evident from the elevated levels of lipid peroxides and decreased activities of membrane-bound enzymes. Disturbances in the electrolyte balance with accumulation of Na+ and Ca2+ and depletion of K+ and Mg2+ were also observed. Administration of Bacoside A inhibited lipid peroxidation, improved the activities of ATPases, and maintained the ionic equilibrium. The results of our study indicate that Bacoside A protects the brain from cigarette smoking induced membrane damage.
Anbarasi
K
K
Department of Biochemistry, University of Madras, Guindy Campus, Chennai--600 025, India. cssdevi@yahoo.com
Vani
G
G
Balakrishna
K
K
Devi
C S Shyamala
CS
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology*; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008672:Metals, Alkali / Q000378:metabolism; D008673:Metals, Alkaline Earth / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014315:Triterpenes / Q000494:pharmacology*
D008672:Metals, Alkali; D008673:Metals, Alkaline Earth; D012503:Saponins; D014028:Tobacco Smoke Pollution; D014315:Triterpenes; C500175:bacoside A; D000251:Adenosine Triphosphatases