title: The hedgehog-PKA pathway regulates two distinct steps of the differentiation of retinal ganglion cells: the cell-cycle exit of retinoblasts and their neuronal maturation.
abstract: In the developing zebrafish retina, neurogenesis is initiated in cells adjacent to the optic stalk and progresses to the entire neural retina. It has been reported that hedgehog (Hh) signalling mediates the progression of the differentiation of retinal ganglion cells (RGCs) in zebrafish. However, the progression of neurogenesis seems to be only mildly delayed by genetic or chemical blockade of the Hh signalling pathway. Here, we show that cAMP-dependent protein kinase (PKA) effectively inhibits the progression of retinal neurogenesis in zebrafish. Almost all retinal cells continue to proliferate when PKA is activated, suggesting that PKA inhibits the cell-cycle exit of retinoblasts. A cyclin-dependent kinase (cdk) inhibitor p27 inhibits the PKA-induced proliferation, suggesting that PKA functions upstream of cyclins and cdk inhibitors. Activation of the Wnt signalling pathway induces the hyperproliferation of retinal cells in zebrafish. The blockade of Wnt signalling inhibits the PKA-induced proliferation, but the activation of Wnt signalling promotes proliferation even in the absence of PKA activity. These observations suggest that PKA inhibits exit from the Wnt-mediated cell cycle rather than stimulates Wnt-mediated cell-cycle progression. PKA is an inhibitor of Hh signalling, and Hh signalling molecule morphants show severe defects in cell-cycle exit of retinoblasts. Together, these data suggest that Hh acts as a short-range signal to induce the cell-cycle exit of retinoblasts. The pulse inhibition of Hh signalling revealed that Hh signalling regulates at least two distinct steps of RGC differentiation: the cell-cycle exit of retinoblasts and RGC maturation. This dual requirement of Hh signalling in RGC differentiation implies that the regulation of a neurogenic wave is more complex in the zebrafish retina than in the Drosophila eye.
authors.affiliation: Masai Initiative Research Unit, RIKEN (The Institute of Physical and Chemical Research, Hirosawa, Saitama, Japan. imasai@postman.riken.jp
authors.forename: Ichiro
authors.initials: I
authors.lastname: Masai
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Noriko
authors.initials: N
authors.lastname: Tonou-Fujimori
authors.forename: Atsuko
authors.initials: A
authors.lastname: Komori
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics; D053823:Hedgehog Proteins; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology; D008938:Mitosis / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D015513:Oncogene Proteins / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D012160:Retina / Q000196:embryology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D051153:Wnt Proteins; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics; D000071676:Zinc Finger Protein GLI1
chemical_list: D004268:DNA-Binding Proteins; D006133:Growth Substances; D053823:Hedgehog Proteins; D036341:Intercellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D043562:Receptors, G-Protein-Coupled; D015534:Trans-Activators; D014157:Transcription Factors; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D000071676:Zinc Finger Protein GLI1; C413414:atoh7 protein, zebrafish; D005576:Colforsin; D017868:Cyclic AMP-Dependent Protein Kinases