[Catfish (Silurus asotus) lectin enhances the cytotoxic effects of doxorubicin].
Rhamnose-binding lectins are widely found in fish eggs. However, their biologic effects on cultured cells are still unknown. Since catfish (Silurus asotus) egg lectin (SAL) bound to globotriaosylceramide (Gb3) expressed on the surface of cells, we analyzed the relationship between Gb3 expression and SAL binding in tumor cell lines using Raji, Daudi, ACHN, P388, and K562 cells. Gb3 was highly expressed on Raji cells but not on K562 cells. SAL bound abundantly to Raji cells but not to K562 cells, and SAL binding depended on the amount of Gb3 on the cell surface. SAL caused a reduction in cell size and increased annexin-V binding to and propidium iodide (PI) incorporation into Raji cells. Although this effect on Raji cells might represent damage at the late apoptosis or necrosis stage, SAL-treated Raji cells remained alive. Thus SAL enhanced PI incorporation into Raji cells without induction of cell death. We examined whether the effects of chemotherapeutic agent(s) are influenced by SAL. SAL increased the incorporation of doxorubicin (Dox) into Raji cells and consequently enhanced the cytotoxic effects of Dox. These results indicate that SAL may induce cell permeability without cytotoxity.
Sugawara
Shigeki
S
Cancer Research Institute, Tohoku Pharmaceutical University, Sendai 981-8558, Japan.
Sasaki
Satoko
S
Ogawa
Yukiko
Y
Hosono
Masahiro
M
Nitta
Kazuo
K
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D004357:Drug Synergism; D029941:Fish Proteins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D011419:Propidium / Q000378:metabolism; D011817:Rabbits; D014281:Trihexosylceramides / Q000378:metabolism; D014407:Tumor Cells, Cultured
D017304:Annexin A5; D000903:Antibiotics, Antineoplastic; D029941:Fish Proteins; D037102:Lectins; D014281:Trihexosylceramides; C402295:rhamnose-binding lectin, catfish; D011419:Propidium; C018549:globotriaosylceramide; D004317:Doxorubicin