title: Quantitative and qualitative evaluation of phytoplankton communities by trichromatic chlorophyll fluorescence excitation with special focus on cyanobacteria.
abstract: We present results that were obtained with a newly developed fluorometer, the 'PhytoSensor'. They are based on multi-wavelength excitation of chlorophyll fluorescence to detect the phytoplankton biomass and to identify main taxons (among cyanobacteria, green and brown microalgae). A method to evaluate the photosynthetic potential of the phytoplankton was established. Attention was focused on the measurement of the cyanobacterial biomass. A modelling to distinguish between the two spectral groups (blue and red) of cyanobacteria as a function of their pigments and physiological status is proposed. The main innovation of the device results in the recording of the fluorescence induction kinetics of the phytoplankton to confirm and refine the evaluation of the taxonomic composition. The PhytoSensor abilities were compared with pigment analysis, commercial fluorometers, particle and microscopic counting and identification. The PhytoSensor has been used with success to monitor the dynamics of phytoplankton in drinking-water supply reservoirs in Southeast Asia.
authors.affiliation: Laboratoire 'Organismes Photosynthétiques et Environnement', CNRS FRE2433, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France.
authors.forename: G
authors.initials: G
authors.lastname: Parésys
authors.forename: C
authors.initials: C
authors.lastname: Rigart
authors.forename: B
authors.initials: B
authors.lastname: Rousseau
authors.forename: A W M
authors.initials: AW
authors.lastname: Wong
authors.forename: F
authors.initials: F
authors.lastname: Fan
authors.forename: J-P
authors.initials: JP
authors.lastname: Barbier
authors.forename: J
authors.initials: J
authors.lastname: Lavaud
mesh_terms: D001208:Asia; D001391:Azo Compounds; D002734:Chlorophyll / Q000032:analysis*; D000458:Cyanobacteria / Q000737:chemistry / Q000378:metabolism*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004801:Eosine Yellowish-(YS); D005453:Fluorescence; D007700:Kinetics; D008739:Methyl Green; D010777:Photochemistry; D010839:Phytoplankton / Q000737:chemistry*; D013997:Time Factors; D014881:Water Supply
chemical_list: D001391:Azo Compounds; C017768:trichrome stain; D002734:Chlorophyll; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)