Automated nanospray using chip-based emitters for the quantitative analysis of pharmaceutical compounds.
An automated nanospray system based on chip technology (the NanoMate) was successfully interfaced to a modified Particle Discriminator Interface on a triple quadrupole mass spectrometer. A number of the interface parameters were optimized to improve the sampling efficiency for ions from the chip-based system. Analytical performance was assessed using a number of biochemicals as well as via a methodology for a pharmaceutical that passed validation as required by Good Laboratory Practices. Infusion analyses in flow rates <1 microL/min provided advantages in terms of throughput and sample consumption when compared to other methodologies based on liquid chromatography.
Corkery
Leonard J
LJ
Eli Lilly Canada Inc., Toronto, Ontario, Canada.
Pang
Henrianna
H
Schneider
Bradley B
BB
Covey
Thomas R
TR
Siu
K W Michael
KW
D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis; D046208:Microchip Analytical Procedures / Q000379:methods*; D036103:Nanotechnology; D004364:Pharmaceutical Preparations / Q000032:analysis*; D043562:Receptors, G-Protein-Coupled / Q000032:analysis; D054440:Receptors, Ghrelin; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D000093542:Gemcitabine
D004364:Pharmaceutical Preparations; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin; D003841:Deoxycytidine; D000093542:Gemcitabine
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