title: Matrix metalloproteinase expression is related to angiogenesis and histologic grade in spindle cell soft tissue neoplasms of the extremities.
abstract: We defined the immunocytochemical expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in benign soft tissue neoplasms, fibromatoses, and sarcomas, together with the activity of gelatinase MMPs and TIMPs measured by zymography and reverse zymography in a subset of cases. The most strongly expressed MMP in all tumors was MMP-1, with weaker expression of MMP-10, MMP-11, and MMP-14 in most tumors. Nuclear expression of MMP-1, MMP-8, and MMP-13 was an unusual feature. TIMP-2 was expressed in all tumors, with stronger expression in fibromatoses than in sarcomas. Fibromatoses and high-grade sarcomas showed greater MMP-1 expression than other groups, and endothelial MMP-2 expression was more extensive in sarcomas. Differences in MMP and TIMP expression might be linked to the biologic behavior of soft tissue neoplasms. The activation of endothelial MMP-2 linked to widespread MMP-14 expression provides a mechanism for sarcomas to modulate their matrix and facilitate angiogenesis.
authors.affiliation: Department of Musculoskeletal Science, University of Liverpool, Liverpool, England.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Roebuck
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Helliwell
authors.forename: Iskander H
authors.initials: IH
authors.lastname: Chaudhry
authors.forename: Socrates
authors.initials: S
authors.lastname: Kalogrianitis
authors.forename: Stuart
authors.initials: S
authors.lastname: Carter
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Kemp
authors.forename: David A
authors.initials: DA
authors.lastname: Ritchie
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Jane
authors.forename: Simon P
authors.initials: SP
authors.lastname: Frostick
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D049109:Cell Proliferation; D005121:Extremities / Q000098:blood supply / Q000473:pathology*; D005350:Fibroma / Q000098:blood supply / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D020782:Matrix Metalloproteinases / Q000145:classification / Q000378:metabolism*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D012509:Sarcoma / Q000098:blood supply / Q000201:enzymology / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000098:blood supply / Q000201:enzymology / Q000473:pathology*; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases