The presence of functional mannose receptor on macrophages at the maternal-fetal interface.
BACKGROUND
The mannose receptor (MR) is involved in the initiation of the immune response and regulation of homeostasis during inflammation and tissue remodeling.


METHODS
Distribution, endocytosis and possible natural ligand tumor associated glycoprotein-72 (TAG-72) for the MR have been examined by immunohistology, immunocytochemistry and flow cytometry at the maternal-fetal interface, characterized by extensive tissue remodeling.


RESULTS
Contrary to disseminated distribution of the MR positive (MR+) cells in term placenta, the MR+ cells of early pregnancy decidua intimately surrounded glands and followed tissue distribution of CD14 positive cells. The mannose receptor was present on freshly isolated first trimester decidual mononuclear cells and distributed mostly on macrophages (77.08 +/- 10.55%, mean +/- SD). The expression of the MR on CD14 positive cells decreased following 18 h culture (P < 0.01) and was accompanied by the reduction of fluorescein isothiocyanate (FITC)-dextran uptake. PAM-1 anti-MR antibody, mannan and TAG-72 reduced FITC-dextran uptake by decidual macrophages.


CONCLUSIONS
These data indicate that the MR+ macrophages, surrounding early decidual glands, are able to internalize ligands for carbohydrate recognition domain of the receptor, including decidual secretory phase mucin TAG-72.
Laskarin
G
G
Department of Physiology and Immunology, School of Medicine, University of Rijeka, B. Branchetta 20/1, 51000 Rijeka, Croatia. Gordana.Laskarin@medri.hr
Cupurdija
K
K
Tokmadzic
V Sotosek
VS
Dorcic
D
D
Dupor
J
J
Juretic
K
K
Strbo
N
N
Crncic
T Bogovic
TB
Marchezi
F
F
Allavena
P
P
Mantovani
A
A
Randic
Lj
Lj
Rukavina
D
D
D000951:Antigens, Neoplasm; D003656:Decidua / Q000276:immunology* / Q000378:metabolism*; D004705:Endocytosis / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D037181:Lectins, C-Type / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D010641:Phenotype; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000276:immunology / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000951:Antigens, Neoplasm; D006023:Glycoproteins; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; C047052:tumor-associated antigen 72; D011374:Progesterone