title: Action and efficacy of p-[131I]iodo-L-phenylalanine on primary human glioma cell cultures and rats with C6-gliomas.
abstract: BACKGROUND
  Recently we demonstrated high selectivity and long retention of p-[123I]iodo-L-phenylalanine (IPA-123) for diagnostic purposes in gliomas. Here we describe the potency of the iodine-131-labelled analogue p-[131I]iodo-L-phenylalanine (IPA-131) for experimental treatment of gliomas.
  
  
  METHODS
  Two human glioma cell cultures and 15/20 rats with C6-gliomas were exposed to IPA-131.
  
  
  RESULTS
  After 24-h-exposure the cell number of the glioma cell cultures was reduced by 77% and 79%, respectively. Four out of five untreated rats died 8-24 days after implantation and had verifiable tumours. Seven animals died despite therapy, while 8 were soon healthier, gained body weight and were sacrificed 81 days after implantation, at which time survival of the treated animals was significantly prolonged (p < 0.05). At autopsy, instead of tumours there were substance defects, surrounded by pre-existing parenchyma infiltrated by probable tumour cells that were not discernible from reactive astrocytes, lymphocytes, monocytes and multinuclear giant cells.
  
  
  CONCLUSIONS
  IPA-131 has promising activity against human glioma cells in vitro and experimental gliomas in vivo.
authors.affiliation: Institute for Neuropathology, University of the Saarland, School of Medicine, D-66421 Homburg, Germany. pabrom@uniklinik-saarland.de
authors.forename: Bernd F M
authors.initials: BF
authors.lastname: Romeike
authors.forename: Dirk
authors.initials: D
authors.lastname: Hellwig
authors.forename: Axel
authors.initials: A
authors.lastname: Heimann
authors.forename: Oliver
authors.initials: O
authors.lastname: Kempski
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Feiden
authors.forename: Carl-Martin
authors.initials: CM
authors.lastname: Kirsch
authors.forename: Samuel
authors.initials: S
authors.lastname: Samnick
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000532:radiotherapy; D005910:Glioma / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D007457:Iodine Radioisotopes / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D008297:Male; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D019275:Radiopharmaceuticals / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D010649:Phenylalanine; C049181:4-iodophenylalanine