Cloning and use of sC as homologous marker for Aspergillus niger transformation.
The sC sequence from Aspergillus niger was cloned and developed into a homologous marker system for genetic transformation. The coding region of the sC gene amplified by PCR from the A. niger genome was provided with Aspergillus nidulans expression signals (gpdA promoter and trpC terminator). This chimeric construct was used to successfully transform a spontaneous sC- isolate of A. niger to prototrophy. The transformants analyzed by Southern analysis showed integration of multiple copies of the transforming DNA. They also exhibited much higher ATP sulfurylase activity than the wild-type A. niger strain reinforcing the molecular data. This demonstrates the usefulness of the sCniger construct, driven by PgpdA, as a marker for A. niger transformation.
Varadarajalu
Lakshmi P
LP
Biotechnology Group, School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, India.
Punekar
Narayan S
NS
D001233:Aspergillus nidulans / Q000235:genetics; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D013430:Sulfate Adenylyltransferase / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic / Q000502:physiology*
D004271:DNA, Fungal; D005819:Genetic Markers; D013430:Sulfate Adenylyltransferase