Separation of mutation avoidance and antirecombination functions in an Escherichia coli mutS mutant.
DNA mismatch repair in Escherichia coli has been shown to be involved in two distinct processes: mutation avoidance, which removes potential mutations arising as replication errors, and antirecombination which prevents recombination between related, but not identical (homeologous), DNA sequences. We show that cells with the mutSDelta800 mutation (which removes the C-terminal 53 amino acids of MutS) on a multicopy plasmid are proficient for mutation avoidance. In interspecies genetic crosses, however, recipients with the mutSDelta800 mutation show increased recombination by up to 280-fold relative to mutS+. The MutSDelta800 protein binds to O6-methylguanine mismatches but not to intrastrand platinated GG cross-links, explaining why dam bacteria with the mutSDelta800 mutation are resistant to cisplatin, but not MNNG, toxicity. The results indicate that the C-terminal end of MutS is necessary for antirecombination and cisplatin sensitization, but less significant for mutation avoidance. The inability of MutSDelta800 to form tetramers may indicate that these are the active form of MutS.
Calmann
Melissa A
MA
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School 364 Plantation Street, Worcester, MA 01605, USA.
Nowosielska
Anetta
A
Marinus
M G
MG
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020137:Base Pair Mismatch; D002945:Cisplatin / Q000633:toxicity; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004265:DNA Helicases / Q000378:metabolism; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D051719:MutS DNA Mismatch-Binding Protein; D009154:Mutation; D009841:Oligonucleotides / Q000378:metabolism; D011995:Recombination, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009841:Oligonucleotides; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli; D004265:DNA Helicases; D002945:Cisplatin
Arch Biochem Biophys. 1957 Mar;67(1):10-5
13412116
DNA Repair (Amst). 2005 Jul 12;4(7):773-81
15925551
J Bacteriol. 2000 Jan;182(2):463-8
10629194
Biochemistry. 2000 Apr 25;39(16):4575-80
10769112
J Biol Chem. 2000 Apr 28;275(17):13017-25
10777605
Oncogene. 2000 Jun 29;19(28):3138-45
10918568
Nature. 2000 Oct 12;407(6805):703-10
11048710
Nature. 2000 Oct 12;407(6805):711-7
11048711
Annu Rev Genet. 2000;34:359-399
11092832
J Mol Biol. 2001 Jan 26;305(4):805-16
11162093
J Biol Chem. 2001 Mar 23;276(12):9413-20
11106642
Mutat Res. 2001 Jul 1;478(1-2):23-43
11406167
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8376-80
11459978
J Cell Physiol. 2002 Apr;191(1):28-41
11920679
DNA Repair (Amst). 2003 Jan 2;2(1):73-89
12509269
DNA Repair (Amst). 2003 Apr 2;2(4):387-405
12606120
J Biol Chem. 2003 Jun 6;278(23):21267-75
12654906
J Biol Chem. 2003 Sep 5;278(36):34667-73
12829697
Annu Rev Microbiol. 2003;57:579-608
14527292
Oncogene. 2004 Jan 8;23(1):260-6
14712231
DNA Repair (Amst). 2004 Jul 2;3(7):719-28
15177181
DNA Repair (Amst). 2004 Aug-Sep;3(8-9):1091-101
15279797
Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14174-9
15375217
J Mol Biol. 1974 May 15;85(2):309-22
4600143
Nature. 1982 Apr 29;296(5860):868-9
7040986
Mol Pharmacol. 1985 Jul;28(1):51-5
3894930
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5057-61
3014530
Nature. 1989 Nov 23;342(6248):396-401
2555716
Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1730-4
1542666
J Biol Chem. 1992 Jun 15;267(17):12142-8
1601880
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3238-41
8159731
J Bacteriol. 1994 Sep;176(17):5393-400
8071216
Nucleic Acids Res. 1996 Feb 15;24(4):640-7
8604304
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6443-7
8692834
Annu Rev Biochem. 1996;65:101-33
8811176
Carcinogenesis. 1996 Sep;17(9):2085-8
8824540
Cancer Res. 1996 Nov 1;56(21):4881-6
8895738
Genetics. 1997 Jan;145(1):29-38
9017387
J Biol Chem. 1999 Feb 26;274(9):5948-52
10026220
J Biol Chem. 1999 Aug 13;274(33):23673-8
10438551