title: Acarbose rearrangement mechanism implied by the kinetic and structural analysis of human pancreatic alpha-amylase in complex with analogues and their elongated counterparts.
abstract: A mechanistic study of the poorly understood pathway by which the inhibitor acarbose is enzymatically rearranged by human pancreatic alpha-amylase has been conducted by structurally examining the binding modes of the related inhibitors isoacarbose and acarviosine-glucose, and by novel kinetic measurements of all three inhibitors under conditions that demonstrate this rearrangement process. Unlike acarbose, isoacarbose has a unique terminal alpha-(1-6) linkage to glucose and is found to be resistant to enzymatic rearrangement. This terminal glucose unit is found to bind in the +3 subsite and for the first time reveals the interactions that occur in this part of the active site cleft with certainty. These results also suggest that the +3 binding subsite may be sufficiently flexible to bind the alpha-(1-6) branch points in polysaccharide substrates, and therefore may play a role in allowing efficient cleavage in the direct vicinity of such junctures. Also found to be resistant to enzymatic rearrangement was acarviosine-glucose, which has one fewer glucose unit than acarbose. Collectively, structural studies of all three inhibitors and the specific cleavage pattern of HPA make it possible to outline the simplest sequence of enzymatic reactions likely involved upon acarbose binding. Prominent features incorporated into the starting structure of acarbose to facilitate the synthesis of the final tightly bound pseudo-pentasaccharide product are the restricted availability of hydrolyzable bonds and the placement of the transition state-like acarviosine group. Additional "in situ" experiments designed to elongate and thereby optimize isoacarbose and acarviosine-glucose inhibition using the activated substrate alphaG3F demonstrate the feasibility of this approach and that the principles outlined for acarbose rearrangement can be used to predict the final products that were obtained.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: Chunmin
authors.initials: C
authors.lastname: Li
authors.forename: Anjuman
authors.initials: A
authors.lastname: Begum
authors.forename: Shin
authors.initials: S
authors.lastname: Numao
authors.forename: Kwan Hwa
authors.initials: KH
authors.lastname: Park
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Withers
authors.forename: Gary D
authors.initials: GD
authors.lastname: Brayer
mesh_terms: D020909:Acarbose / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism*; D000606:Amino Sugars / Q000096:biosynthesis / Q000737:chemistry; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry; D010179:Pancreas / Q000201:enzymology*; D013379:Substrate Specificity; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000606:Amino Sugars; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; C493349:isoacarbose; C493350:acarviosine-glucose; C076099:acarviosine; D000516:alpha-Amylases; D020909:Acarbose