Monitoring of pollution of air fine particles (PM2.5) and study on their genetic toxicity.
OBJECTIVE
To compare PM2.5 pollation level between the city of coal-fuel pollution (Taiyuan) and the city of pollution mixed with coal fuels and vehicle exhausts (Beijing), to analyze the concentration of B[a]p and Pb in the pollutants, and to study the DNA damage by PM2.5.


METHODS
Air fine particles (PM2.5) were collected in Beijing and Taiyuan by means of the filter membrane method, the concentration of B[a]p and Pb were analyzed by high performance liquid chromatography and atomic absorption spectroscopy respectveily, and the damage of DNA by PM2.5 was detected by single cell gel-electrophoresis (SCGE) using the human lung epithelial cells (A549) as target cells.


RESULTS
The concentration of PM2.5 in the winter of Beijing was 0.028-0.436 mg/m(3), and that in Taiyuan was 0.132-0.681 mg/m(3). The concentration of B[a]p was 0.104 and 0.156 microg/mg on PM2.5 of Beijing and Taiyuan, respectively, whereas the concentration of Pb was 1.094 and 1.137 microg/mg on PM2.5 of Beijing and Taiyuan, respectively. Exposure to PM2.5 at the concentrations of 5, 50, and 200 microg/mL for 12 h and 24 h caused DNA damage of the human alveolar epithelium, and the ratios of the tailing and length of the tail were all significantly different from those of the negative control group (P < 0.05), and indicated a dose-response relationship.


CONCLUSIONS
PM2.5 has certain genetic toxicity.
Xu
Dong-Qun
DQ
Institute for Environmental Health and Related Products Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. dongqunxu@yahoo.com.cn
Zhang
Wen-Li
WL
D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000633:toxicity*; D002681:China; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D064420:Drug-Related Side Effects and Adverse Reactions; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D007854:Lead / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D010316:Particle Size; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism; D012621:Seasons
D000393:Air Pollutants; D007854:Lead; D001564:Benzo(a)pyrene