title: Maternal transmission ratio distortion at the mouse Om locus results from meiotic drive at the second meiotic division.
abstract: We have observed maternal transmission ratio distortion (TRD) in favor of DDK alleles at the Ovum mutant (Om) locus on mouse chromosome 11 among the offspring of (C57BL/6 x DDK) F(1) females and C57BL/6 males. Although significant lethality occurs in this backcross ( approximately 50%), differences in the level of TRD found in recombinant vs. nonrecombinant chromosomes among offspring argue that TRD is due to nonrandom segregation of chromatids at the second meiotic division, i.e., true meiotic drive. We tested this hypothesis directly, by determining the centromere and Om genotypes of individual chromatids in zygote stage embryos. We found similar levels of TRD in favor of DDK alleles at Om in the female pronucleus and TRD in favor of C57BL/6 alleles at Om in the second polar body. In those embryos for which complete dyads have been reconstructed, TRD was present only in those inheriting heteromorphic dyads. These results demonstrate that meiotic drive occurs at MII and that preferential death of one genotypic class of embryo does not play a large role in the TRD.
authors.affiliation: Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
authors.forename: Guangming
authors.initials: G
authors.lastname: Wu
authors.forename: Lanping
authors.initials: L
authors.lastname: Hao
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Han
authors.forename: Shaorong
authors.initials: S
authors.lastname: Gao
authors.forename: Keith E
authors.initials: KE
authors.lastname: Latham
authors.forename: Fernando Pardo-Manuel
authors.initials: FP
authors.lastname: de Villena
authors.forename: Carmen
authors.initials: C
authors.lastname: Sapienza
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D008297:Male; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000166:cytology*; D015053:Zygote / Q000166:cytology*
references.citation: Mamm Genome. 1999 May;10(5):492-7
references.pmid: 10337624
references.citation: Mol Hum Reprod. 1997 Aug;3(8):693-8
references.pmid: 9294853
references.citation: Genetics. 2000 Jan;154(1):351-6
references.pmid: 10628994
references.citation: Genetics. 2000 May;155(1):283-9
references.pmid: 10790402
references.citation: Genetics. 2000 Oct;156(2):775-83
references.pmid: 11014823
references.citation: Mamm Genome. 2001 May;12(5):331-9
references.pmid: 11331939
references.citation: J Cell Sci. 2001 Jul;114(Pt 13):2417-26
references.pmid: 11559750
references.citation: Genetics. 2001 Nov;159(3):1179-89
references.pmid: 11729161
references.citation: Genetics. 2002 Mar;160(3):1123-31
references.pmid: 11901128
references.citation: Genetics. 2002 Aug;161(4):1651-9
references.pmid: 12196408
references.citation: J Reprod Fertil. 1973 May;33(2):283-91
references.pmid: 4736011
references.citation: J Reprod Fertil. 1974 Nov;41(1):85-96
references.pmid: 4431024
references.citation: J Reprod Fertil. 1986 Mar;76(2):779-81
references.pmid: 3754584
references.citation: Proc Natl Acad Sci U S A. 1986 Sep;83(18):6883-6
references.pmid: 3462735
references.citation: J Reprod Fertil. 1989 Jul;86(2):679-88
references.pmid: 2760894
references.citation: Genet Res. 1990 Apr;55(2):97-100
references.pmid: 2164490
references.citation: Dev Suppl. 1990;:81-7
references.pmid: 2090434
references.citation: Genetics. 1992 Sep;132(1):241-6
references.pmid: 1398057
references.citation: J Med Genet. 1993 Nov;30(11):903-9
references.pmid: 8301643
references.citation: Genetics. 1996 Apr;142(4):1299-304
references.pmid: 8846906
references.citation: Mamm Genome. 1996 Feb;7(2):114-6
references.pmid: 8835527
references.citation: Mamm Genome. 1997 Sep;8(9):642-6
references.pmid: 9271664
references.citation: Genetics. 2000 Jan;154(1):333-42
references.pmid: 10628992