Activin A inhibits differentiation of 3T3-L1 preadipocyte.
We investigated the effect of activin A on differentiation of 3T3-L1 preadipocyte. Activin A suppressed the induction of terminal differentiation markers such as glycerol-3-phosphate dehydrogenase (GPDH) activity, lipid accumulation, and the expression of adipocyte fatty acid-binding protein (aP2) mRNA when the cells were treated with activin A throughout the differentiation period. Activin A treatment during the early phase decreased GPDH activity and aP2 mRNA level, and also reduced the expression of peroxisome proliferator-activated receptor (PPAR) gamma and CCAAT/enhancer binding protein (C/EBP) alpha mRNAs without affecting the expressions of the active isoforms of C/EBPbeta and its mRNA. On the other hand, activin A treatment had no effect on the mitotic clonal expansion. These results indicate that activin A inhibits adipogenesis via affecting the transcriptional factor cascade upstream of PPARgamma expression.
Hirai
Shizuka
S
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Sakyo-ku, Kyoto-shi 606-8502, Japan. shizuka@kais.kyoto-u.ac.jp
Yamanaka
Mai
M
Kawachi
Hiroyuki
H
Matsui
Tohru
T
Yano
Hideo
H
D041721:3T3-L1 Cells; D028341:Activins / Q000494:pharmacology*; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D028322:Inhibin-beta Subunits / Q000494:pharmacology*; D051379:Mice; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D014157:Transcription Factors / Q000187:drug effects
D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; C422405:activin A; D028341:Activins; D028322:Inhibin-beta Subunits; D005993:Glycerolphosphate Dehydrogenase