title: Fluorescence quenching mechanism of a polyphenylene polyelectrolyte with other macromolecules: cytochrome c and dendrimers.
abstract: This study investigates the fluorescence quenching of a polyphenyl based polyelectrolyte by positively charged macromolecules (proteins and dendrimers). This work shows that the fluorescence quenching of the dendrimer materials does not involve energy transfer or electron transfer but is correlated to the overall charge on the dendrimer and its size. The quenching is hypothesized to result from conformational changes that occur upon binding the polyelectrolyte to the protein or dendrimer. This mechanism is qualitatively different from that invoked for small-molecule analytes.
authors.affiliation: Chemistry Department, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Palwinder
authors.initials: P
authors.lastname: Kaur
authors.forename: D H
authors.initials: DH
authors.lastname: Waldeck
authors.forename: Cuihua
authors.initials: C
authors.lastname: Xue
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
mesh_terms: D045304:Cytochromes c / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D050091:Dendrimers; D004573:Electrolytes; D011108:Polymers; D045304:Cytochromes c; C041325:polyphenylene sulfide