[Preparation of porcine acellular dermal matrix by low concentration of trypsin digestion and repeated freeze-thaw cycles].
OBJECTIVE
To establish a new method for the preparation of porcine acellular dermal matrix.


METHODS
The antigenicity of the porcine dermis was weakened by removing epidermal and dermal cells from the porcine skin through the digestion with low-concentration trypsin and repeated freeze-thaw cycles. Split thickness porcine skin was treated with 0.05% trypsin to remove the cells from the epidermis and dermis. Repeated freeze-thaw cycles were employed to further weed out the residual cells within the dermis. The prepared acellular dermis was then examined grossly, as well as histologically, and also by immunohistochemical method.


RESULTS
No cell could be identified in the prepared porcine acellular dermal matrix. The integral basement membrane was preserved on the surface of dermal matrix with compact dermal matrix collagen structure.


CONCLUSIONS
Low concentration trypsinization and repeated freeze-thaw cycles seemed to be a simple and effective method for the preparation of xenogeneic acellular dermal matrix.
Tan
Qian
Q
Department of Plastic Surgery, Gulou Hospital, The Medical School of Nanjing University, Nanjing 210008, P.R. China.
Zou
Zhong-tao
ZT
Ning
Guan-sen
GS
Lin
Zi-hao
ZH
Zhou
Hong-reng
HR
Liang
Zhi-wei
ZW
Chen
Xi
X
Wu
Jian-ming
JM
D000818:Animals; D020405:Dermis / Q000166:cytology* / Q000637:transplantation; D005109:Extracellular Matrix / Q000637:transplantation / Q000648:ultrastructure*; D005615:Freezing; D016038:Skin Transplantation; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014357:Trypsin / Q000008:administration & dosage*
D014357:Trypsin