RANTES G-401A polymorphism is associated with allergen sensitization and FEV1 in Chinese children.
G-401A polymorphism in RANTES promoter was associated with near-fatal asthma and atopic dermatitis in children. We studied whether gain-of-function mutations in RANTES gene were associated with asthma and atopy-related traits in Chinese children. Plasma total and aeroallergen-specific IgE concentrations were measured using micro-particle immunoassay and fluorescent enzyme immunoassay, respectively. Restriction fragment length polymorphism was used to genotype RANTES G-401A and C-28G. One hundred and twenty-nine asthmatic children and 66 controls were recruited. Their mean logarithmic plasma total IgE concentrations were 2.53 and 1.98, respectively (P<0.0001). RANTES G-401A was not associated with physician-diagnosed asthma (P = 0.408). However, RANTES G-401A allele was significantly associated with IgE sensitization to cat (odds ratio 2.35; 95% CI 1.15-4.77; P = 0.010). Those homozygous for -401A had higher plasma cat-specific IgE levels (P = 0.034). Subjects having -401A were also more likely to have mold-specific IgE (odds ratio 3.82; 95% CI 1.24-12.14; P = 0.007). On spirometry, those with -401A/ A had lower forced expiratory volume in 1-s (FEV1; P = 0.044). RANTES C-28G was not associated with any outcome in this study. In conclusion, the gain-of-function mutation at -401 of RANTES promoter is associated with sensitization to cat and mold allergens and FEV1 in Chinese children.
Leung
T F
TF
Department of Paediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong SAR, China. leung2142@cuhk.edu.hk
Tang
N L S
NL
Lam
C W K
CW
Li
A M
AM
Fung
S L M
SL
Chan
I H S
IH
Wong
G W K
GW
D000293:Adolescent; D000485:Allergens / Q000009:adverse effects*; D001249:Asthma / Q000235:genetics*; D018946:Chemokine CCL5 / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D020022:Genetic Predisposition to Disease; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D009154:Mutation / Q000235:genetics; D011110:Polymorphism, Genetic; D012130:Respiratory Hypersensitivity / Q000235:genetics*; D013147:Spirometry / Q000379:methods
D000485:Allergens; D018946:Chemokine CCL5; D007073:Immunoglobulin E