Approaching the self-incompatibility locus Z in rye (Secale cereale L.) via comparative genetics.
Using barley and wheat expressed sequence tags as well as rice genomic sequence and mapping information, we revisited the genomic region encompassing the self-incompatibility (SI) locus Z on rye chromosome 2RL applying a comparative approach. We were able to arrange 12 novel sequence-tagged site (STS) markers around Z, spanning a genetic distance of 32.3 cM, with the closest flanking markers mapping at a distance of 0.5 cM and 1.0 cM from Z, respectively, and one marker cosegregating with Z, in a testcross population of 204 progeny. Two overlapping rice bacterial artifical chromosomes (BACs), OSJNBa0070O11 and OSJNBa0010D21, were found to carry rice orthologs of the three rye STS markers from the 1.5-cM interval encompassing Z. The STS-marker orthologs on these rice BACs span less than 125,000 bp of the rice genome. The STS marker TC116908 cosegregated with Z in a mapping population and revealed a high degree of polymorphism among a random sample of rye plants of various origin. TC116908 was shown via Southern hybridization to correspond to gene no. 10 (OSJNBa0070O11.10) on rice BAC OSJNBa0070O11. Reverse transcription-PCR with a TC116908-specific primer pair resulted in the amplification of a fragment of the expected size from the rye pistil but not from leaf cDNA. OSJNBa0070O11.10 was found to show a highly significant sequence similarity to AtUBP22, a ubiquitin-specific protease (UBP). TC116908 likely represents a putative UBP gene that is specifically expressed in rye pistils and cosegregates with Z. Given that the ubiquitination of proteins is emerging as a general mechanism involved in different SI systems of plants, TC116908 appears to be a promising target for further investigation with respect to its relation to the SI system of the grasses.
Hackauf
B
B
Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Rudolf-Schick-Platz 3a, 18190, Gross Lüsewitz, Germany.
Wehling
P
P
D015139:Blotting, Southern; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D019295:Computational Biology; D003433:Crosses, Genetic; D017931:DNA Primers; D010450:Endopeptidases / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D012275:Oryza / Q000235:genetics; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012434:Secale / Q000235:genetics*; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics; D064570:Ubiquitin-Specific Proteases; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers; D005819:Genetic Markers; D010450:Endopeptidases; D064570:Ubiquitin-Specific Proteases
Trends Plant Sci. 2003 Dec;8(12):606-13
14659710
Funct Integr Genomics. 2004 Mar;4(1):59-66
14752608
J Biol Chem. 1992 Jan 15;267(2):719-27
1309773
Nature. 2004 May 20;429(6989):305-9
15152254
Science. 1998 Oct 23;282(5389):656-9
9784118
Mamm Genome. 2001 Dec;12(12):930-2
11707780
J Biochem. 2003 Jul;134(1):9-18
12944365
Crit Rev Biochem Mol Biol. 1998;33(5):337-52
9827704
Science. 2002 Apr 5;296(5565):92-100
11935018
Funct Integr Genomics. 2004 Mar;4(1):47-58
14767678
Theor Appl Genet. 1985 Dec;71(2):289-91
24247396
Mol Gen Genet. 1995 Oct 25;248(6):744-54
7476878
Science. 2002 Apr 5;296(5565):79-92
11935017
Nucleic Acids Res. 1995 Jul 25;23(14):2729-33
7651834
Science. 2003 Jul 18;301(5631):376-9
12869764
Genome. 1998 Jun;41(3):328-36
9729767
Plant Cell. 2003 Apr;15(4):885-98
12671085
Genome Res. 2003 Aug;13(8):1818-27
12902377
Heredity (Edinb). 2002 May;88(5):385-90
11986876
Genetics. 2003 Jun;164(2):673-83
12807788
Genome. 2004 Apr;47(2):231-8
15060575
Trends Plant Sci. 2003 Dec;8(12):598-605
14659709
Cancer Res. 2003 Feb 1;63(3):682-8
12566314
Methods Mol Biol. 2000;132:365-86
10547847
Curr Biol. 1995 Jul 1;5(7):737-9
7583118
Ann Bot. 2002 Jan;89(1):3-10
12096816
Genome. 1996 Apr;39(2):379-94
18469901
J Hered. 2002 Jan-Feb;93(1):77-8
12011185
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
Plant Cell. 2004 Mar;16(3):582-95
14973168
Mol Genet Genomics. 2004 May;271(4):426-36
15007733
Theor Appl Genet. 1982 Sep;63(3):245-8
24270823
Proc Natl Acad Sci U S A. 1984 Dec;81(24):8014-8
6096873
Genetics. 2002 Jun;161(2):825-34
12072477
Biotechniques. 1990 Feb;8(2):172-3
2317372