Simple method to grow enteric campylobacters in unsupplemented liquid medium without the need for microaerophilic kits.
Campylobacter strains (100 human, animal and environmental isolates) were grown in untreated brain heart infusion broth medium (10 ml in tightly capped 20 ml capacity universal tubes) without using microaerophilic kits. Cells grown in these conditions did not differ in their growth rates, protein profiles or substrate utilisation even after 40 passages compared to cells grown under microaerophilic conditions. Growth in such conditions provides a cost effective, convenient and simple system for growing pure culture of campylobacters and obviates the generation of microaerobic conditions using specialised kits.
Mohammed
Khairallah A S
KA
Department of Life Sciences, Franklin-Wilkins Building, King's College London, 150 Stamford Street, London SE1 9NN, UK.
Miles
Roger J
RJ
Halablab
Mahmoud A
MA
D000332:Aerobiosis; D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002167:Campylobacter / Q000254:growth & development* / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media; D006801:Humans
D003470:Culture Media