[Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte].
OBJECTIVE
To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte.


METHODS
The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours, the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using beta-actin as internal reference.


RESULTS
The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only (P < 0.05), the effect of berberine was inhibited by high concentration insulin (P < 0.05).


CONCLUSIONS
In vitro, berberine increases the expression of adiponectin in 3T3-L1 adipocyte, insulin inhibits the effect of berberine.
Gu
Wei
W
The 2nd Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009, China. guwei@aiocrm.com
Zeng
Wen-heng
WH
Hu
Hai-ying
HY
D041721:3T3-L1 Cells / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D052242:Adiponectin; D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D052242:Adiponectin; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D001599:Berberine