title: Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9.
abstract: Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5alpha. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.
authors.affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan, Republic of China. cychen@ntu.edu.tw.
authors.forename: Chien-Jui
authors.initials: CJ
authors.lastname: Huang
authors.forename: Tang-Kai
authors.initials: TK
authors.lastname: Wang
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Chung
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D002688:Chitinases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D027762:Lilium / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D013624:Taiwan
chemical_list: D000935:Antifungal Agents; D002688:Chitinases