Investigation of the stability of polyelectrolyte multilayer coatings in open-tubular capillary electrochromatography using laser scanning confocal microscopy.
A simple polyelectrolyte multilayer (PEM) coating procedure was used for the development of stable modified capillaries. PEM coatings were constructed in fused-silica capillaries using alternating rinses of cationic and anionic polyelectrolytes. The multilayer coatings investigated in this study consisted of two and twenty layer pairs, or bilayers. A bilayer is one layer of a cationic polymer and one layer of an anionic polymer. Poly(diallyldimethylammonium chloride) was used as the cationic polymer, and the polymeric surfactant poly(sodium N-undecanoyl-L-leucylvalinate) was used as the anionic polymer. Previous studies for both chiral and achiral separations have shown that PEM-coated capillaries have excellent reproducibilities, remarkable endurance, and strong stabilities against extreme pH values when used in open-tubular capillary electrochromatography (OT-CEC). In this study, the stability of the coatings was further investigated after exposure to 0.1 M and 1.0 M NaOH. Structural changes of these coatings were monitored using laser scanning confocal microscopy (LSCM) after flushing the capillaries with NaOH. This technique allowed observation of the degradation of the coatings. Observations are discussed in terms of separations using OT-CEC. Electropherograms obtained from the chiral separation of 1,1'-binaphthyl-2,2'-dihydrogenphosphate in OT-CEC showed a decrease in selectivity and an increase in electroosmotic mobility after long exposure to NaOH. The ability to recover the capillaries by exposure to NaOH was also demonstrated. Measurements of electroosmotic mobility and selectivity showed that 2-bilayer and 20-bilayer PEM coatings could be completely removed from the capillary surface after approximately 3.5 and 9.5 h, respectively, of continuous exposure to 1 M NaOH.
Kapnissi-Christodoulou
Constantina P
CP
Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA.
Lowry
Mark
M
Department of Chemistry, University of Iowa, Iowa City, IA, USA.
Agbaria
Rezik A
RA
Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA.
Geng
Lei
L
Department of Chemistry, University of Iowa, Iowa City, IA, USA.
Warner
Isiah M
IM
Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA.
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D004355:Drug Stability; D004563:Electrochemistry; D018613:Microscopy, Confocal; D009995:Osmosis; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
D011506:Proteins; D013501:Surface-Active Agents; C111877:poly(sodium N-undecylenyl-leucine-valine)
Decher, G., Schmitt, J., Prog. Colloid Polym. Sci. 1992, 89, 160-164.
Decher, G., Science 1997, 277, 1232-1237.
Schlenoff, J. B., Dubas, S. T., Farhat, T., Langmuir 2000, 16, 9968-9969.
Smith, R. N., Reven, L., Barrett, C. J., Macromolecules 2003, 36, 1876-1881.
Schlenoff, J. B., Ly, H., Li, M., J. Am. Chem. Soc. 1998, 120, 7626-7634.
Farhat, T. R., Schlenoff, J. B., Langmuir 2001, 17, 1184-1192.
Graul, T. W., Schlenoff, J. B., Anal. Chem. 1999, 71, 4007-4013.
Kapnissi, C. P., Akbay, C., Schlenoff, J. B., Warner, I. M., Anal. Chem. 2002, 74, 2328-2335.
Kamande, M. W., Kapnissi, C. P., Zhu, X., Akbay, C., Warner, I. M., Electrophoresis 2003, 24, 945-951.
Kapnissi, C. P., Valle, B. C., Warner, I. M., Anal. Chem. 2003, 75, 6097-6104.
Rmaile, H. H., Schlenoff, J. B., J. Am. Chem. Soc. 2003, 125, 6602-6603.
Müller, M., Biomacromolecules 2001, 2, 262-269.
Laurent, D., Schlenoff, J. B., Langmuir 1997, 13, 1552-1557.
Schlenoff, J. B., Dubas, S. T., Macromolecules 2001, 34, 592-598.
Caruso, F., Lichtenfeld, H., Donath, E., Möhwald, H., Macromolecules 1999, 32, 2317-2328.
Rmaile, H. H., Schlenoff, J. B., Langmuir 2002, 18, 8263-8265.
Castelnovo, M., Joanny, J.-F., Langmuir 2000, 16, 7524-7532.
Dubas, S. T., Schlenoff, J. B., Macromolecules 2001, 34, 3736-3740.
Dubas, S. T., Schlenoff, J. B., Macromolecules 1999, 32, 8153-8160.
Hayes, J. D., Malik, A., Anal. Chem. 2001, 73, 987-996.
Farhat, T., Yassin, G., Dubas, S. T., Schlenoff, J. B., Langmuir 1999, 15, 6621-6623.
Sui, Z., Salloum, D., Schlenoff, J. B., Langmuir 2003, 19, 2491-2505.
Katayama, H., Ishihama, Y., Asakawa, N., Anal. Chem. 1998, 70, 2254-2260.
Katayama, H., Ishihama, Y., Asakawa, N., Anal. Chem. 1998, 70, 5272-5277.
Lowry, M., He, Y., Geng, L., Anal. Chem. 2002, 74, 1811-1818.
Tallarek, U., Rapp, E., Sann, H., Reichl, U., Seidel-Morgenstern, A., Langmuir 2003, 19, 4527-4531.
Tallerek, U., Paces, M., Rapp, E., Electrophoresis 2003, 24, 4241-4253.
Zhu, H., Ji, J., Tan, Q., Barbosa, M. A., Shen, J., Biomacromolecules 2003, 4, 378-386.
Moya, S., Donath, E., Sukhorukov, G. B., Auch, M., Bäumler, H., Lichtenfeld, H., Möhwald, H., Macromolecules 2000, 33, 4538-4544.
Guo, H. X., Heinämäki, J., Yliruusi, J., Int. J. Pharmaceutics 1999, 186, 99-108.
Silvano, D., Krol, S., Diaspro, A., Cavalleri, O., Gliozzi, A., Microsc. Res. Techn. 2002, 59, 536-541.
Georgieva, R., Moya, S., Leporatti, S., Neu, B., Bäumler, H., Reichle, C., Donath, E., Möhwald, H., Langmuir 2000, 16, 7075-7081.
Voigt, A., Lichtenfeld, H., Sukhorukov, G. B., Zastrow, H., Donath, E., Bäumler, H., Möhwald, H., Ind. Eng. Chem. Res. 1999, 38, 4037-4043.
Lamprecht, A., Schäfer, U. F., Lehr, C.-M., Eur. J. Pharmaceut. Biopharmaceut. 2000, 49, 1-9.
Stricker, S. A., Whitaker, M., Microsc. Res. Techn. 1999, 46, 356-369.
Gao, C., Leporatti, S., Donath, E., Möhwald, H., J. Phys. Chem. B 2000, 104, 7144-7149.
Buttino, I., Ianora, A., Carotenuto, Y., Zupo, V., Miralto, A., Microsc. Res. Techn. 2003, 60, 458-464.
Khopade, A. J., Caruso, F., Langmuir 2003, 19, 6219-6225.
Astrakharchik-Farrimond, E., Shekunov, B. Y., Sawyer, N. B. E., Morgan, S. P., Somekh, M. G., See, C. W., Particle and Particle Systems Characterization 2003, 20, 104-110.
Missert, N., Copeland, R. G., Barbour, J. C., Mikkalson, J. E., Electrochem. Soc. Proc. 2001, 22, 686-690.
Caponetti, G., Hrkach, J. S., Kriwet, B., Poh, M., Lotan, N., Colombo, P., Langer, R., J. Pharm. Sci. 1999, 88, 136-141.
Wang, J., Warner, I. M., J. Chromatogr. A 1995, 711, 297-304.
Chiari, M., Nesi, M., Righetti, P. G., in: Righetti, P. G. (Ed.), Capillary Electrophoresis in Analytical Biotechnology, CRC Press, Boca Raton, FL 1996, pp.  1-36.
Chankvetadze, B., Capillary Electrophoresis in Chiral Analysis, John Wiley & Sons, London 1997, pp.  1-72.
Skoog, D. A., Holler, J. F., Nieman, T. A., in: Sherman, M., Bortel, J., Messina, F. (Eds.), Principles of Instrumental Analysis, 5th ed., Harcourt Brace College Publishers, Orlando, FL 1998, pp.  674-700.
Zhu, X., Kamande, M. W., Thiam, S., Kapnissi, C. P., Mwongela, S. M., Warner, I. M., Electrophoresis 2004, 25, 562-568.