Varied patterns of DNA methylation change between different satellite regions in bovine preimplantation development.
Global reduction of DNA methylation, a part of genome reprogramming processes, occurs in a gradual manner until before implantation and is recognized as a conserved process in mammals. Here, we reported that in bovine, satellite regions exhibited varied patterns of methylation changes when one-cell egg advanced to the blastocyst; a maintenance methylation was observed in satellite I sequences, a decrease in alpha satellites, and an increase in satellite II regions. Cloned embryos exhibited similar changes for DNA methylation in the satellite I and alpha. We also observed that the satellite I and alpha sequences were methylated more in inner cell mass region of the blastocyst whereas the satellite II showed selective demethylation in this region. Together, these findings point that individual satellite sequences carry their own methylation patterns under the pressure of global demethylation, suggesting that local methylation control system acts on the satellite regions in early bovine embryos.
Kang
Yong-Kook
YK
Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
Lee
Hyo-Jong
HJ
Shim
Jung-Jae
JJ
Yeo
Seungeun
S
Kim
Seok-Ho
SH
Koo
Deog-Bon
DB
Lee
Kyung-Kwang
KK
Beyhan
Zeki
Z
First
Neal L
NL
Han
Yong-Mahn
YM
D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D019175:DNA Methylation; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics*; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data
D004276:DNA, Satellite