Rab11 mediates post-Golgi trafficking of rhodopsin to the photosensitive apical membrane of Drosophila photoreceptors.
In developing Drosophila photoreceptors, rhodopsin is trafficked to the rhabdomere, a specialized domain within the apical membrane surface. Rab11, a small GTPase implicated in membrane traffic, immunolocalizes to the trans-Golgi network, cytoplasmic vesicles and tubules, and the base of rhabdomeres. One hour after release from the endoplasmic reticulum, rhodopsin colocalizes with Rab11 in vesicles at the base of the rhabdomere. When Rab11 activity is reduced by three different genetic procedures, rhabdomere morphogenesis is inhibited and rhodopsin-bearing vesicles proliferate within the cytosol. Rab11 activity is also essential for development of MVB endosomal compartments; this is probably a secondary consequence of impaired rhabdomere development. Furthermore, Rab11 is required for transport of TRP, another rhabdomeric protein, and for development of specialized membrane structures within Garland cells. These results establish a role for Rab11 in the post-Golgi transport of rhodopsin and of other proteins to the rhabdomeric membranes of photoreceptors, and in analogous transport processes in other cells.
Satoh
Akiko K
AK
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
O'Tousa
Joseph E
JE
Ozaki
Koichi
K
Ready
Donald F
DF
D000818:Animals; D030801:Animals, Genetically Modified; D015220:Calcium Channels / Q000096:biosynthesis / Q000235:genetics; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics; D004705:Endocytosis / Q000502:physiology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D012243:Rhodopsin / Q000378:metabolism*; D050051:Transient Receptor Potential Channels; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
D015220:Calcium Channels; D029721:Drosophila Proteins; D012243:Rhodopsin; D050051:Transient Receptor Potential Channels; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C063169:trp protein, Drosophila