title: Analysis of IGG and IGG4 in HIV-1 seropositive patients and correlation with biological and genetic markers.
abstract: We have compared the levels of immunoglobulins G (IgG) and G4 (IgG4) in extreme seropositive patients from the GRIV cohort consisting of 168 patients with slow progression (SP) and 60 with rapid progression (RP) as well as in 173 healthy controls. IgG levels were significantly higher in SP patients than in RP patients (P = 0.008), both higher than in seronegative individuals. IgG4 levels were significantly lower in SP patients than in RP patients (P = 0.001), both lower than in seronegative individuals. We tried to correlate these levels with biological parameters (CD4(+) and CD8(+) cells, total lymphocytes, white blood cell counts, percentage of CD4(+) cells, and viral load) as well as with genetic markers from Th1/Th2 cytokines (IL2, IL4, IL6, IL10, IL13, and IFNgamma). IgG levels were correlated with the percentage of CD4(+) cells in SP while IgG4 levels were correlated with CD8(+) cell count in SP and with percentage of CD4(+) cells in RP patients. Among the parameters measured in SP patients at the time of inclusion in the study, the best predictor of progression towards AIDS was the viral load, the best predictor for stability was CD4(+) cell count, but overall, the best predictor for SP evolution (stability vs. progression) appeared to be the percentage of CD4(+) cells. Interestingly, correlations between the levels of IgG or IgG4 and the cytokine gene polymorphisms were found, notably in the IL10 gene.
authors.affiliation: Immunochimie, Hôpital Tenon, Inserm E-0209 and Université Pierre et Marie Curie, Paris, France.
authors.forename: Aicha
authors.initials: A
authors.lastname: Abbas
authors.forename: Alexandre
authors.initials: A
authors.lastname: Vasilescu
authors.forename: Hervé
authors.initials: H
authors.lastname: Do
authors.forename: Houria
authors.initials: H
authors.lastname: Hendel
authors.forename: Mustapha
authors.initials: M
authors.lastname: Maachi
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Goutalier
authors.forename: Emmanuel G
authors.initials: EG
authors.lastname: Regulier
authors.forename: Jay
authors.initials: J
authors.lastname: Rappaport
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Matsuda
authors.forename: Amu
authors.initials: A
authors.lastname: Therwath
authors.forename: Pierre
authors.initials: P
authors.lastname: Aucouturier
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Zagury
mesh_terms: D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D005500:Follow-Up Studies; D005819:Genetic Markers / Q000235:genetics; D015658:HIV Infections / Q000235:genetics / Q000276:immunology*; D006679:HIV Seropositivity / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000235:genetics; D008297:Male
chemical_list: D015415:Biomarkers; D005819:Genetic Markers; D007074:Immunoglobulin G