title: Identification of functional apple scab resistance gene promoters.
abstract: Apple scab (Venturia inaequalis) is one of the most damaging diseases affecting commercial apple production. Some wild Malus species possess resistance against apple scab. One gene, HcrVf2, from a cluster of three genes derived from the wild apple Malus floribunda clone 821, has recently been shown to confer resistance to apple scab when transferred into a scab-susceptible apple variety. For this proof-of-function experiment, the use of the 35S promoter from Cauliflower mosaic virus was reliable and appropriate. However, in order to reduce the amount of non-plant DNA in genetically modified apple to a minimum, with the aim of increasing genetically modified organism acceptability, these genes would ideally be regulated by their own promoters. In this study, sequences from the promoter region of the three members of the HcrVf gene family were compared. Promoter constructs containing progressive 5' deletions were prepared and used for functional analyses. Qualitative assessment confirmed promoter activity in apple. Quantitative promoter comparison was carried out in tobacco (Nicotiana glutinosa) and led to the identification of several promoter regions with different strengths from a basal level to half the strength of the 35S promoter from Cauliflower mosaic virus.
authors.affiliation: Plant Pathology, Institute of Plant Science, Swiss Federal Institute of Technology, ETH Zurich, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Silfverberg-Dilworth
authors.forename: S
authors.initials: S
authors.lastname: Besse
authors.forename: R
authors.initials: R
authors.lastname: Paris
authors.forename: E
authors.initials: E
authors.lastname: Belfanti
authors.forename: S
authors.initials: S
authors.lastname: Tartarini
authors.forename: S
authors.initials: S
authors.lastname: Sansavini
authors.forename: A
authors.initials: A
authors.lastname: Patocchi
authors.forename: C
authors.initials: C
authors.lastname: Gessler
mesh_terms: D001203:Ascomycota; D001483:Base Sequence; D017931:DNA Primers; D040461:Gene Components; D017343:Genes, Plant / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D027845:Malus / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012231:Rhizobium; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014026:Nicotiana / Q000235:genetics
chemical_list: D017931:DNA Primers
references.citation: Gene. 1997 Oct 24;200(1-2):107-16
references.pmid: 9373144
references.citation: Plant Cell Rep. 1993 Aug;12(10):559-63
references.pmid: 24201785
references.citation: Mol Gen Genet. 1992 Jan;231(2):226-32
references.pmid: 1736093
references.citation: Plant Cell Rep. 1994 Jul;13(10):587-93
references.pmid: 24196227
references.citation: Mol Gen Genet. 1989 Jan;215(2):337-44
references.pmid: 2651885
references.citation: Plant J. 1998 Dec;16(6):651-9
references.pmid: 10069073
references.citation: Genetics. 2002 Dec;162(4):1995-2006
references.pmid: 12524365
references.citation: Plant Mol Biol. 2001 Mar;45(5):577-85
references.pmid: 11414616
references.citation: Mol Plant Microbe Interact. 2001 Apr;14(4):508-15
references.pmid: 11310738
references.citation: EMBO J. 1987 Dec 20;6(13):3901-7
references.pmid: 3327686
references.citation: EMBO J. 1990 Nov;9(11):3415-27
references.pmid: 2209551
references.citation: Nucleic Acids Res. 1988 Oct 25;16(20):9877
references.pmid: 3186459
references.citation: Planta. 2000 Jan;210(2):232-40
references.pmid: 10664129
references.citation: Plant J. 1999 Jan;17(2):209-14
references.pmid: 10074718
references.citation: J Biol Chem. 1992 Nov 25;267(33):23515-9
references.pmid: 1429696
references.citation: Plant J. 2000 Jun;22(6):543-51
references.pmid: 10886774
references.citation: Plant Cell. 1997 Aug;9(8):1251-64
references.pmid: 9286104
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: EMBO J. 1996 Oct 15;15(20):5690-700
references.pmid: 8896462
references.citation: Plant Physiol. 2002 Jul;129(3):1138-49
references.pmid: 12114568
references.citation: Nucleic Acids Res. 1999 Jan 1;27(1):297-300
references.pmid: 9847208
references.citation: Planta. 2001 Sep;213(5):731-40
references.pmid: 11678277
references.citation: Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):886-90
references.pmid: 14715897