Myoepithelial cell markers in salivary gland neoplasms.
We compared the immunoexpression of 5 myoepithelial cell (MEC) markers (alpha-smooth-muscle actin, calponin, h-caldesmon, vimentin, and S-100-protein) using 16 pleomorphic adenomas (PA), 15 adenoid cystic carcinomas (ACC), and 3 epithelial-myoepithelial carcinomas (EMC) of salivary glands. The alpha-smooth-muscle actin was useful for identification of MECs, especially in cribriform and tubular ACC, followed by EMC. Calponin was similar to alpha-smooth-muscle actin, except for polygonal and plasmacytoid cells of PA and for solid ACC, which showed alpha-smooth-muscle actin negative and calponin positive. H-caldesmon was negative. Vimentin immunostained all MEC types, and was negative in luminal cells. S-100 protein was expressed both in the nuclei and cytoplasm of MECs and luminal cells, especially in PA. The best way to identify MEC is using alpha-smooth-muscle actin or calponin, plus vimentin, since in tumors MECs are hardly ever fully differentiated.
Furuse
Cristiane
C
Department of Oral Pathology, São Leopoldo Mandic Dental Research Institute, Campinas, Brazil.
Sousa
Suzana O Machado de
SO
Nunes
Fábio Daumas
FD
Magalhães
Marina Helena Cury Gallottini de
MH
Araújo
Vera Cavalcanti de
VC
D000199:Actins / Q000378:metabolism; D008949:Adenoma, Pleomorphic / Q000378:metabolism* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008840:Microfilament Proteins; D008875:Middle Aged; D009208:Myoepithelioma / Q000378:metabolism* / Q000473:pathology; D009418:S100 Proteins / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D014746:Vimentin / Q000378:metabolism; D000096985:Calponins
D000199:Actins; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D009418:S100 Proteins; D014746:Vimentin; D000096985:Calponins