Deletion of the multiple-drug efflux pump AcrAB in Escherichia coli prolongs the postantibiotic effect.
The mechanism of the postantibiotic effect (PAE) was examined in Escherichia coli. Drugs exhibited longer-lasting PAEs in an acrAB mutant, suggesting that intracellular drug concentrations influence the duration of the PAE. With specific assays for tetracycline and erythromycin, a direct link between intracellular persistence of antibiotics and maintenance of the PAE was established.
Stubbings
William
W
Antimicrobial Research Centre and School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, UK.
Bostock
Julieanne
J
Ingham
Eileen
E
Chopra
Ian
I
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002352:Carrier Proteins / Q000502:physiology*; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000502:physiology*; D008074:Lipoproteins / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology*; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D013752:Tetracycline / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D004917:Erythromycin; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D013752:Tetracycline; C083497:AcrA protein, E coli; C083496:AcrB protein, E coli
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