Biotechnological production and applications of coenzyme Q10.
Coenzyme Q10 is widely used as an essential component of ATP generation in the oxidative phosphorylation process and as an antioxidant preventing lipid peroxidation and scavenging superoxide. It is also recommended as a supplement to 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors. Research efforts on the production of coenzyme Q10 by microorganisms focus on the development of potent strains by conventional mutagenesis and metabolic engineering, analysis and modification of the key metabolic pathways and optimization of fermentation strategies. Especially, random mutants with drugs resistance show a high coenzyme Q10 concentration. Metabolic engineering techniques have been applied to improve coenzyme Q10 production. The key enzymes involved in the coenzyme Q10 biosynthesis pathway have been cloned and expressed in Escherichia coli. The rational design of metabolic pathways in combination with engineering optimization of fermentation processes could facilitate the development of viable bioconversion processes.
Choi
Jin-Ho
JH
School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul, 151-742, South Korea.
Ryu
Yeon-Woo
YW
Seo
Jin-Ho
JH
D000975:Antioxidants / Q000627:therapeutic use; D001709:Biotechnology; D002318:Cardiovascular Diseases / Q000188:drug therapy; D003067:Coenzymes; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008956:Models, Chemical; D015394:Molecular Structure; D030781:Organisms, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000627:therapeutic use
D000975:Antioxidants; D003067:Coenzymes; D011994:Recombinant Proteins; D014451:Ubiquinone; C024989:coenzyme Q10