Molecular epidemiology and prevalence of macrolide efflux genes mef(A) and mef(E) in Streptococcus pneumoniae obtained in Canada from 1997 to 2002.
One hundred forty M phenotype Streptococcus pneumoniae isolates were evaluated by PCR-restriction fragment length polymorphism, serotyping, and pulsed-field gel electrophoresis. Molecular genotyping revealed that the predominant macrolide resistance mechanism in S. pneumoniae in Canada is mef(E) and resistance dissemination is due to both spread of the genetic element MEGA as well as clonal dissemination of penicillin- and/or macrolide-resistant strains.
Wierzbowski
Aleksandra K
AK
Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Manitoba, Canada.
Swedlo
Dean
D
Boyd
Dave
D
Mulvey
Michael
M
Nichol
Kim A
KA
Hoban
Daryl J
DJ
Zhanel
George G
GG
D001426:Bacterial Proteins / Q000235:genetics*; D002170:Canada; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D018942:Macrolides / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*; D013997:Time Factors
D001426:Bacterial Proteins; D018942:Macrolides; C117866:MefA protein, Streptococcus; D008565:Membrane Proteins
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