title: Influence of the organic matrix on root dentine erosion by citric acid.
abstract: This investigation was conducted in an attempt to clarify the role of the organic matrix in bovine dentine demineralisation by 1% citric acid. Dentine slabs (n = 15) were treated for 2 min with 10% sodium hypochlorite (NaOCl, a strong protein solvent), 2% glutaraldehyde (GDA, a protein fixative) or deionised water (DIW, as negative control) prior to each of 5 demineralisation periods of 30 min. The mineral loss (DeltaZ), lesion depth (LD) and surface loss (SL) were determined after each period of demineralisation, by transverse microradiography. The NaOCl-treated group showed higher DeltaZ and LD than the GDA- and DIW-treated groups. No differences between GDA and DIW groups were found. The DeltaZ and LD profiles were explained by quadratic fits (r(2) >0.80; p = 0.001) in all groups. SL was detected only in the NaOCl-treated group. The data suggested that the maintenance of the organic matrix in the lesion might be important to reduce the erosion progression rate.
authors.affiliation: Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
authors.forename: A T
authors.initials: AT
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Ando
authors.forename: J A
authors.initials: JA
authors.lastname: Cury
authors.forename: M C
authors.initials: MC
authors.lastname: Serra
authors.forename: C
authors.initials: C
authors.lastname: González-Cabezas
authors.forename: D T
authors.initials: DT
authors.lastname: Zero
mesh_terms: D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000009:adverse effects*; D019343:Citric Acid / Q000009:adverse effects*; D003804:Dentin / Q000473:pathology*; D018450:Disease Progression; D005404:Fixatives / Q000009:adverse effects; D005976:Glutaral / Q000009:adverse effects; D008852:Microradiography; D008903:Minerals / Q000032:analysis; D011897:Random Allocation; D012973:Sodium Hypochlorite / Q000009:adverse effects; D012997:Solvents / Q000009:adverse effects; D017001:Tooth Demineralization / Q000139:chemically induced / Q000473:pathology; D014077:Tooth Erosion / Q000139:chemically induced / Q000473:pathology*; D014092:Tooth Root / Q000473:pathology*
chemical_list: D002614:Chelating Agents; D005404:Fixatives; D008903:Minerals; D012997:Solvents; D019343:Citric Acid; D012973:Sodium Hypochlorite; D005976:Glutaral