title: The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice.
abstract: The quality control of sperm is critical for efficient reproduction. In germ cells, cell death involves different processes to those in somatic cells, and in many cases, the trigger to induce cell death in deficient germ cells is still unclear. It is known that the fatty acid composition of sperm is related to fertility. Composition of the fatty acid of germ cells changes dynamically during spermatogenesis, and fatty acid binding protein (FABP) may be involved in these changes. In this study, we developed transgenic mice with a testicular germ-cell-specific FABP (PERF15) transgene, whose expression was controlled by the Cre-LoxP site-specific recombination system. We also developed transgenic mice with the Cre gene under the control of the spermatocyte specific Pgk2 promoter. In double transgenic mice, following Cre-mediated recombination of the PERF15 containing transgene, PERF15 was strongly overexpressed. Its overexpression induced multinucleate symplasts to form, indicating programmed germ cell death occurred at the elongated spermatid stage. As a result, sperm harboring the transgene were significantly decreased, but the surviving sperm demonstrated higher fertility than natural sperm. Therefore, we conclude that PERF15 associate with the direction of germ cell fates and preserve the quality of sperm.
authors.affiliation: Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kido
authors.forename: Satoru
authors.initials: S
authors.lastname: Arata
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Suzuki
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Hosono
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Miyazaki
authors.forename: Izumu
authors.initials: I
authors.lastname: Saito
authors.forename: Toshio
authors.initials: T
authors.lastname: Kuroki
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism*; D017931:DNA Primers; D050556:Fatty Acid-Binding Proteins; D005298:Fertility / Q000235:genetics; D049452:Green Fluorescent Proteins; D015151:Immunoblotting; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010735:Phosphoglycerate Kinase / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology*; D019076:Transgenes / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; C484252:Fabp9 protein, mouse; D050556:Fatty Acid-Binding Proteins; D007527:Isoenzymes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D010735:Phosphoglycerate Kinase; C104317:phosphoglycerate kinase, testis specific; C045073:Cre recombinase; D019426:Integrases