Utrophin is lacking at the neuromuscular junctions in the extraocular muscles of normal cat: artefact or true?
Extraocular muscles (EOM) are typically spared in Duchenne muscular dystrophy. We hypothesized that this might be due to different patterns of utrophin expression. The expression of utrophin was examined in EOM of normal cats using immunohistochemical methods and Western blot. For detecting acetylcholine receptors (AChR), we used alpha-bungarotoxin. Surprisingly, alpha-bungarotoxin failed to stain the AChR and no expression of utrophin could be detected at the neuromuscular junctions. Our study could indicate that the expression of utrophin is dependent on the structure of the AChR.
Assadi
Maziar
M
Institute of Anatomy, University of Zürich-Irchel, Zürich, Switzerland. maziar.asadi@roche.com
Müntener
Markus
M
D000818:Animals; D001808:Blood Vessels / Q000737:chemistry; D015153:Blotting, Western; D002415:Cats; D006614:Hindlimb; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D009469:Neuromuscular Junction / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry; D009801:Oculomotor Muscles / Q000098:blood supply / Q000737:chemistry* / Q000294:innervation; D051381:Rats; D011950:Receptors, Cholinergic / Q000032:analysis; D049411:Utrophin / Q000032:analysis*
D011950:Receptors, Cholinergic; D049411:Utrophin
J Cell Sci. 1998 Jul;111 ( Pt 13):1801-11
9625743
J Cell Biol. 1992 Oct;119(2):357-66
1400579
Ann Neurol. 1992 Oct;32(4):586-8
1456746
J Exp Biol. 2002 Oct;205(Pt 20):3133-42
12235193
Curr Opin Neurobiol. 1997 Dec;7(6):880-7
9464973
Muscle Nerve. 1988 Oct;11(10):1056-64
3185600
Neurosci Lett. 1999 Oct 8;273(3):143-6
10515179
J Histochem Cytochem. 1964 Mar;12:219-21
14187330
Science. 1995 Nov 3;270(5237):755-6
7481760
Science. 1980 Jun 27;208(4451):1454-6
7384786
Biophys J. 2003 Aug;85(2):943-53
12885641
Am J Pathol. 1999 Apr;154(4):1017-22
10233840
Muscle Nerve. 1988 Aug;11(8):795-803
3173406
Annu Rev Med. 1997;48:457-66
9046976
Invest Ophthalmol Vis Sci. 2000 Aug;41(9):2422-31
10937549
Nature. 1988 Jul 14;334(6178):154-6
3290691
Acta Neuropathol. 1993;85(3):256-9
8460531
Brain Res Dev Brain Res. 2000 Feb 7;119(2):289-95
10675780
J Biol Chem. 1990 Oct 5;265(28):16717-20
2170350
J Neurol Sci. 1996 Aug;139(2):180-6
8856650
Cell Mol Life Sci. 2002 Aug;59(8):1296-316
12363034
Invest Ophthalmol Vis Sci. 2003 May;44(5):1918-26
12714624
J Hered. 1988 Nov-Dec;79(6):405-8
3209849
Neuroreport. 1992 Oct;3(10):857-60
1421088
Neuron. 1993 Jul;11(1):53-66
8338668
J Neurobiol. 2003 Feb 15;54(3):486-501
12532399
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3243-7
2014247
J Neurol Sci. 1992 Jul;110(1-2):149-59
1506854
J Neuropathol Exp Neurol. 1993 Mar;52(2):119-28
8440993
Neuromuscul Disord. 1994 Sep-Nov;4(5-6):433-45
7881288
Neuron. 2001 Oct 25;32(2):265-75
11683996
Am J Pathol. 1989 Nov;135(5):909-19
2683799
J Cell Biol. 1991 Dec;115(6):1695-700
1757469
J Exp Med. 1995 Aug 1;182(2):467-75
7629506
Neuron. 1989 Aug;3(2):163-75
2560390
Br Med Bull. 1989 Jul;45(3):703-18
2688824
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1189-92
6583703
J Biol Chem. 2002 Apr 5;277(14):12406-17
11790782
J Neuropathol Exp Neurol. 1998 Aug;57(8):780-90
9720493