[Determination of changes in membrane phospholipids of rat lung and liver tissues from acute heat stress by high performance liquid chromatography].
The changes in the contents of phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) in lung and liver membrane tissues of rats suffered from acute heat stress were investigated with HPLC. The separation was performed on a mu-Porasil Si column, 300 mm x 3.9 mm i.d., by isocratic elution of acetonitrile : methanol : 85% phosphoric acid (100 : 3 : 1). The phospholipids were detected at 205 nm. The results showed that the contents (mean +/- SD) of PI, PS, PE and PC in normal rats liver were 270.0 +/- 18.2 microg/g, 133.7 +/- 8.6 microg/g, 1465 +/- 132 microg/g and 53.7 +/- 2.8 mg/g respectively, whereas those in heat-stressed rats whose rectal temperature was increased to 42 degrees C and maintained for 15 minutes were decreased by 14%-25% (P<0.01) except PS. When the heat-stressed rats recovered to room temperature for 4h, the contents of phospholipids returned to normal level. The changes of PS and PC in rat lung tissues were identical with those in liver tissues.
Zhou
J
J
National Center of Biomedical Analysis, Beijing, 100850.
Liao
J
J
Xing
C
C
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018882:Heat Stress Disorders / Q000378:metabolism*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008168:Lung / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008563:Membrane Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D008563:Membrane Lipids; D010743:Phospholipids