Regulation of neuropilin 1 by spinal cord injury in adult rats.
Using RT-PCR, in situ hybridization, Western blotting, and immunofluorescence, we have analyzed the expression of neuropilin 1 (Np1) in two models of spinal cord injury (spinal cord hemisection and dorsal column crush) and following dorsal root rhizotomy in adult rats. Our results show that Np1 RNA and protein are up-regulated in the spinal cord after all these lesions but remain unaltered in the adjacent dorsal root ganglia. In control animals, Np1 levels in the spinal cord are low and appear to be localized mainly in blood vessels, motoneurons, and in the superficial layers of the dorsal horn. After DCC and rhizotomy, Np1 is expressed de novo around the injury and in the deafferentated dorsal horn, respectively, mainly by OX42-positive microglial cells. Both lesions affect the sensory projections, and interestingly a consistent increase of Np1 signal is additionally seen in the dorsal horn where these projections terminate. Unexpectedly, this increase is bilateral after unilateral rhizotomy.
Agudo
Marta
M
Neuro Restoration CARD, Wolfson Centre Guy's Campus, King's College London, London Bridge, London SE1 1UL, UK. martabar@um.es
Robinson
Michelle
M
Cafferty
William
W
Bradbury
Elizabeth J
EJ
Kilkenny
Carol
C
Hunt
Stephen P
SP
McMahon
Stephen B
SB
D000818:Animals; D019771:Axotomy; D004195:Disease Models, Animal; D007839:Functional Laterality / Q000502:physiology; D017628:Microglia / Q000166:cytology / Q000378:metabolism; D009409:Nerve Crush; D039942:Neuropilin-1 / Q000235:genetics / Q000378:metabolism*; D020671:Posterior Horn Cells / Q000166:cytology / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019051:Rhizotomy; D013116:Spinal Cord / Q000378:metabolism* / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000503:physiopathology; D013126:Spinal Nerve Roots / Q000378:metabolism* / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology*
D012333:RNA, Messenger; D039942:Neuropilin-1