title: Kurarinone isolated from Sophora flavescens Ait inhibited MCP-1-induced chemotaxis.
abstract: The accumulation of circulating monocytes in the arterial wall is an early in atherosclerotic plaque formation. Monocyte chemoattractant protein-1 (MCP-1) promotes the migration of monocytes and would play a role in the development of atherosclerotic lesions. Searching for inhibitors of MCP-1-induced cell migration from natural sources, we isolated one active compound through active-guided fractionations from the MeOH extracts of Sophora flavescens Ait (Leguminosae). On the basis of spectral evidence, the structure of active compound was identified as kurarinone. It inhibited the migration of THP-1 cells induced by MCP-1 with IC50 value of 19.2 microg/mL. In addition, it inhibited the binding of MCP-1 to THP-1 cells and phosphorylation of p42/44 MARK.
authors.affiliation: Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology, 52 Eoun-dong, Taejeon 305-333, Republic of Korea.
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Nam
authors.forename: Oh Eok
authors.initials: OE
authors.lastname: Kwon
authors.forename: Jin Ah
authors.initials: JA
authors.lastname: Baek
authors.forename: Jong Sun
authors.initials: JS
authors.lastname: Chang
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.forename: Young Kook
authors.initials: YK
authors.lastname: Kim
mesh_terms: D002460:Cell Line; D002464:Cell Migration Inhibition; D018932:Chemokine CCL2 / Q000037:antagonists & inhibitors* / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D029909:Sophora
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D005419:Flavonoids; D010936:Plant Extracts; C411319:kurarinone