The structure of the O-polysaccharide from Pseudomonas stutzeri OX1 containing two different 4-acylamido-4,6-dideoxy-residues, tomosamine and perosamine.
The structure of the O-polysaccharide from the lipopolysaccharide of Pseudomonas stutzeri OX1 was determined by chemical procedures and by 1D and 2D NMR spectroscopy. The analysis revealed the presence of a heterogeneous polymer made by 4-acetamido-4,6-dideoxy-D-mannopyranose (D-Rhap4NAc) and 4-formamido-4,6-dideoxy-D-galactopyranose (d-Fucp4NFo). The combination of chemical and NMR analyses indicates that the heterogeneity of the polymer depends on its non-stoichiometric glycosylation by Fuc4NFo, as shown below: [formula: see text]. The structure of the heterogeneous polymer was confirmed by Smith degradation that significantly simplified the structure of the O-polysaccharide, allowing for the isolation and identification of a linear homopolymer of Rhap4NAc.
Leone
Serena
S
Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Via Cintia 4, I-80126 Naples, Italy.
Izzo
Viviana
V
Lanzetta
Rosa
R
Molinaro
Antonio
A
Parrilli
Michelangelo
M
Di Donato
Alberto
A
D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry; D008358:Mannose / Q000031:analogs & derivatives* / Q000737:chemistry*; D008745:Methylation; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification*; D044223:Pseudomonas stutzeri / Q000737:chemistry*
D008070:Lipopolysaccharides; D019081:O Antigens; C057033:4-amino-4,6-dideoxy-D-mannose; D008358:Mannose