Interferon-gamma inhibits in vitro mobilization of eosinophils by interleukin-5.
BACKGROUND
Th2 cytokines play pivotal roles in allergic inflammation, including eosinophilia, and their actions are antagonized by Th1 cytokines, conferring them therapeutic potential.


METHODS
In this study, we examined the ability of a number of cytokines to suppress the activation of eosinophils that function as effector cells for allergic airway diseases.


RESULTS
Interleukin (IL)-5, IL-6, and tumor necrosis factor (TNF) induced an eosinophil shape change, whereas interferon (IFN)-gamma significantly inhibited the shape change. Other cytokines, including IL-1beta, IL-4, IL-10 and IL-13, had little or only slightly enhancing or reducing effects on the shape change. We further analyzed the IFN-gamma effect, showing that pretreatment with IFN-gamma strongly suppressed IL-5-induced eosinophil shape change, and cycloheximide (CHX) abrogated the suppression by IFN-gamma, suggesting that new protein synthesis is required for the inhibitory effect by this cytokine. In agreement with these results, IFN-gamma blocked the eosinophil migration and ERK phophorylation induced by IL-5, and the addition of CHX restored eosinophil chemotaxis.


CONCLUSIONS
Collectively, IFN-gamma may attenuate eosinophilic inflammation by directly negating eosinophil mobilization.
Park
Choon-Sik
CS
Genome Research Center for Asthma and Allergic Diseases, Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital, Seoul and Bucheon, Korea.
Choi
Eun Nam
EN
Kim
Jung Sun
JS
Choi
Yun Sung
YS
Rhim
Tai Youn
TY
Chang
Hun Soo
HS
Chung
Il Yup
IY
D048430:Cell Shape / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects* / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000494:pharmacology*; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000276:immunology; D010766:Phosphorylation / Q000187:drug effects; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D016207:Cytokines; D048049:Extracellular Signal-Regulated MAP Kinases