Unique cooperative binding interaction observed between a minor groove binding Pt antitumor agent and Hoechst dye 33258.
The trinuclear compound [{Pt(NH3)3}2mu-{trans-Pt(NH3)2(H2N(CH2)6NH2)}2(6+) (0,0,0/t,t,t) binds to DNA only through noncovalent hydrogen bonding and electrostatic interactions. The presence of this 6+ cation allows discrimination of binding modes for common DNA ligands: binding of minor-groove agents such as Hoechst 33258 is cooperative, and dye-DNA interaction is enhanced whereas intercalation as exemplified by ethidium bromide is competitively inhibited.
Harris
Amanda
A
Department of Chemistry, Virginia Commonwealth University, 1001 W. Main Street, Richmond, Virginia 23284-2006, USA.
Qu
Yun
Y
Farrell
Nicholas
N
D000494:Allosteric Regulation; D000970:Antineoplastic Agents / Q000737:chemistry*; D006690:Bisbenzimidazole / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000737:chemistry*
D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D009944:Organoplatinum Compounds; C059267:dichlorotetraamine(1,6-hexamethylenediamine)diplatinum(II); D004247:DNA; C114062:BBR 3464; D006690:Bisbenzimidazole