title: Auxin-dependent cell division and cell elongation. 1-Naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid activate different pathways.
abstract: During exponential phase, the tobacco (Nicotiana tabacum) cell line cv Virginia Bright Italia-0 divides axially to produce linear cell files of distinct polarity. This axial division is controlled by exogenous auxin. We used exponential tobacco cv Virginia Bright Italia-0 cells to dissect early auxin signaling, with cell division and cell elongation as physiological markers. Experiments with 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) demonstrated that these 2 auxin species affect cell division and cell elongation differentially; NAA stimulates cell elongation at concentrations that are much lower than those required to stimulate cell division. In contrast, 2,4-D promotes cell division but not cell elongation. Pertussis toxin, a blocker of heterotrimeric G-proteins, inhibits the stimulation of cell division by 2,4-D but does not affect cell elongation. Aluminum tetrafluoride, an activator of the G-proteins, can induce cell division at NAA concentrations that are not permissive for division and even in the absence of any exogenous auxin. The data are discussed in a model where the two different auxins activate two different pathways for the control of cell division and cell elongation.
authors.affiliation: Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy. campanoni@ibba.cnr.it
authors.forename: Prisca
authors.initials: P
authors.lastname: Campanoni
authors.forename: Peter
authors.initials: P
authors.lastname: Nick
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D049108:Cell Enlargement / Q000187:drug effects*; D002460:Cell Line; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D009280:Naphthaleneacetic Acids / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000166:cytology*
chemical_list: D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D011956:Receptors, Cell Surface; C083845:endoplasmic reticulum auxin-binding protein 4, Zea mays; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid
references.citation: Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11493-7
references.pmid: 8265579
references.citation: Plant Physiol. 1977 Oct;60(4):585-91
references.pmid: 16660143
references.citation: Curr Opin Plant Biol. 2001 Feb;4(1):57-62
references.pmid: 11163169
references.citation: Plant Physiol. 2004 Feb;134(2):735-47
references.pmid: 14764902
references.citation: Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8925-9
references.pmid: 1924352
references.citation: Plant J. 2001 Dec;28(6):607-17
references.pmid: 11851907
references.citation: Plant Sci. 2001 Jul;161(2):249-258
references.pmid: 11448755
references.citation: Plant J. 2001 Sep;27(6):591-9
references.pmid: 11576442
references.citation: New Phytol. 1995 Feb;129(2):167-201
references.pmid: 33874562
references.citation: FEBS Lett. 1999 Apr 16;449(1):57-60
references.pmid: 10225428
references.citation: J Biol Chem. 1999 Oct 1;274(40):28314-20
references.pmid: 10497189
references.citation: Plant Cell Physiol. 2000 Apr;41(4):503-8
references.pmid: 10845464
references.citation: Genes Dev. 2001 Apr 1;15(7):902-11
references.pmid: 11297513
references.citation: Planta. 1972 Dec;107(4):325-40
references.pmid: 24477482
references.citation: Proc Natl Acad Sci U S A. 1989 Feb;86(3):891-5
references.pmid: 16594015
references.citation: FEBS Lett. 1997 May 26;408(3):315-8
references.pmid: 9188784
references.citation: Plant Physiol. 2003 Nov;133(3):1251-60
references.pmid: 14612587
references.citation: Planta. 1976 Jan;130(1):7-13
references.pmid: 24424536
references.citation: Plant Physiol. 1977 Mar;59(3):357-64
references.pmid: 16659851
references.citation: Plant Cell Physiol. 1998 May;39(5):492-9
references.pmid: 9664714
references.citation: Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7208-12
references.pmid: 1323130
references.citation: Planta. 1996 Apr;198(4):532-541
references.pmid: 28321663
references.citation: Science. 1998 Nov 6;282(5391):1114-7
references.pmid: 9804548
references.citation: Plant Physiol. 2000 Oct;124(2):693-702
references.pmid: 11027718
references.citation: Plant Cell Rep. 1996 Nov;16(1-2):76-9
references.pmid: 24178659
references.citation: EMBO J. 2002 Jun 17;21(12):2877-85
references.pmid: 12065401
references.citation: Science. 2001 Jun 15;292(5524):2066-9
references.pmid: 11408654
references.citation: Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6310-4
references.pmid: 8022777
references.citation: Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2210-5
references.pmid: 9482864
references.citation: Plant Mol Biol. 1997 Mar;33(4):679-89
references.pmid: 9132059
references.citation: Genes Dev. 1995 Sep 1;9(17):2131-42
references.pmid: 7657165
references.citation: FEBS Lett. 1985 Oct 28;191(2):181-5
references.pmid: 3863758
references.citation: Development. 2000 Jan;127(1):23-32
references.pmid: 10654597
references.citation: Plant Cell. 2003 Feb;15(2):393-409
references.pmid: 12566580
references.citation: Curr Biol. 2003 Aug 19;13(16):1418-22
references.pmid: 12932326
references.citation: Plant Cell. 2001 Jul;13(7):1639-52
references.pmid: 11449056
references.citation: Planta. 2002 Dec;216(2):293-301
references.pmid: 12447543
references.citation: Plant Mol Biol. 2002 Jun-Jul;49(3-4):339-48
references.pmid: 12036258
references.citation: Biochem Soc Trans. 1992 Feb;20(1):59-63
references.pmid: 1321760