title: FISH-aimed karyotyping and characterization of Renner complexes in permanent heterozygote Rhoeo spathacea.
abstract: Fluorescence in situ hybridization (FISH) using 25S rDNA, 5S rDNA, and telomere sequences as probes was carried out in the complex permanent heterozygote Rhoeo spathacea. Telomere sites were exclusively terminal. All 10 25S rDNA loci were located distally and appeared transcriptionally active after silver staining. Six distal and 2 interstitial 5S rDNA sites were detected; 2 of the distal sites strictly colocalized with 25S rDNA loci. The 2 intercalary 5S rDNA loci occurred in short arms of 2 chromosomes that conjoined at meiosis. Chromosomes differed as to the amount of AT-rich centric heterochromatin, suggesting involvement of pericentromeric regions in translocations. The possibility of Robertsonian-like rearrangements was discussed. Double target FISH with ribosomal probes along with DAPI fluorescence gave the basis for full chromosome identification in mitosis. The 2 Renner complexes are structurally balanced, both having 5 25S and 4 5S rDNA sites. Centromere clustering, telomere association, a high number of NOR sites, and a strong tendency for formation of joint nucleoli contribute to the preservation of highly polarized Rabl arrangement at interphase. These findings were discussed in relation to meiotic catenation in Rhoeo.
authors.affiliation: Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Kraków, Poland. h.golczyk@iphils.uj.edu.pl
authors.forename: Hieronim
authors.initials: H
authors.lastname: Golczyk
authors.forename: Robert
authors.initials: R
authors.lastname: Hasterok
authors.forename: Andrzej J
authors.initials: AJ
authors.lastname: Joachimiak
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D006579:Heterozygote; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008540:Meiosis / Q000235:genetics; D034823:Tradescantia / Q000235:genetics*; D014178:Translocation, Genetic / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal