title: A mutation in the decoding center of Thermus thermophilus 16S rRNA suggests a novel mechanism of streptomycin resistance.
abstract: A spontaneous kanamycin resistance and capreomycin resistance mutation, A1408G, in the decoding center of 16S rRNA, was identified in the extreme thermophile Thermus thermophilus. Unexpectedly, this mutation also confers resistance to streptomycin. We propose a novel mechanism of streptomycin resistance by which A1408G influences conformational changes in 16S rRNA during tRNA selection.
authors.affiliation: Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02912, USA.
authors.forename: Steven T
authors.initials: ST
authors.lastname: Gregory
authors.forename: Jennifer F
authors.initials: JF
authors.lastname: Carr
authors.forename: Albert E
authors.initials: AE
authors.lastname: Dahlberg
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D010641:Phenotype; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology*; D016963:Thermus thermophilus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013307:Streptomycin
references.citation: Mol Microbiol. 1999 Jan;31(1):53-8
references.pmid: 9987109
references.citation: J Bacteriol. 1986 Apr;166(1):338-40
references.pmid: 3957870
references.citation: J Infect Dis. 1998 Jun;177(6):1573-81
references.pmid: 9607835
references.citation: Chem Biol. 2002 Jun;9(6):747-55
references.pmid: 12079787
references.citation: Nat Struct Mol Biol. 2004 Apr;11(4):316-22
references.pmid: 15004548
references.citation: Cell. 2002 Nov 27;111(5):721-32
references.pmid: 12464183
references.citation: EMBO J. 1999 Jun 1;18(11):3133-8
references.pmid: 10357824
references.citation: Nature. 2000 Sep 21;407(6802):327-39
references.pmid: 11014182
references.citation: J Mol Biol. 2001 Jun 1;309(2):333-8
references.pmid: 11371156
references.citation: J Bacteriol. 2001 Jul;183(14):4382-5
references.pmid: 11418580
references.citation: J Clin Microbiol. 1998 May;36(5):1220-5
references.pmid: 9574680
references.citation: Genetics. 1989 Oct;123(2):281-92
references.pmid: 2583478
references.citation: Nucleic Acids Res. 2004;32(10):3220-7
references.pmid: 15199170
references.citation: Appl Environ Microbiol. 1986 Dec;52(6):1313-6
references.pmid: 16347236
references.citation: Science. 1996 Nov 22;274(5291):1367-71
references.pmid: 8910275
references.citation: J Bacteriol. 1951 May;61(5):549-50
references.pmid: 14832197
references.citation: Nucleic Acids Res. 1984 Feb 24;12(4):2055-60
references.pmid: 6322133
references.citation: J Bacteriol. 1997 Aug;179(15):4795-801
references.pmid: 9244267
references.citation: Nature. 2000 Sep 21;407(6802):340-8
references.pmid: 11014183
references.citation: FEBS Lett. 2001 Oct 12;506(3):231-4
references.pmid: 11602251
references.citation: J Mol Biol. 2001 Mar 9;306(5):1023-35
references.pmid: 11237616
references.citation: Nucleic Acids Res. 1998 Jan 1;26(1):280-4
references.pmid: 9399853
references.citation: Trends Biochem Sci. 2003 May;28(5):259-66
references.pmid: 12765838