title: Homing phenotypes of tumor-specific CD8 T cells are predetermined at the tumor site by crosspresenting APCs.
abstract: Expression of tissue-specific homing molecules directs antigen-experienced T cells to particular peripheral tissues. In studies using soluble antigens that focused on skin and gut, antigen-presenting cells (APCs) within regional lymphoid tissues were proposed to be responsible for imprinting homing phenotypes. Whether this occurs in other sites and after physiologic antigen processing and presentation is unknown. We define in vivo imprinting of distinct homing phenotypes on monospecific T cells responding to antigens expressed by tumors in intracerebral, subcutaneous, and intraperitoneal sites with efficient brain-tropism of CD8 T cells crossprimed in the cervical lymph nodes (LNs). Multiple imprinting programs could occur simultaneously in the same LN when tumors were present in more than one site. Thus, the identity of the LN is not paramount in determining the homing phenotype; this critical functional parameter is dictated upstream at the site of antigen capture by crosspresenting APCs.
authors.affiliation: Laboratory of Tumour Immunology, Division of Oncology, Geneva University Hospital, Rue Micheli-du-Crest 24, CH-1211 Geneva 14, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Calzascia
authors.forename: Frédérick
authors.initials: F
authors.lastname: Masson
authors.forename: Wilma
authors.initials: W
authors.lastname: Di Berardino-Besson
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Contassot
authors.forename: Rick
authors.initials: R
authors.lastname: Wilmotte
authors.forename: Michel
authors.initials: M
authors.lastname: Aurrand-Lions
authors.forename: Curzio
authors.initials: C
authors.lastname: Rüegg
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Dietrich
authors.forename: Paul R
authors.initials: PR
authors.lastname: Walker
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D002490:Central Nervous System / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules