Gene expression in gastric biopsies from patients infected with Helicobacter pylori.
BACKGROUND
Helicobacter pylori infection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. In this study the effects of H. pylori infection on host gene expression in the gastric antral mucosa in patients were examined.


METHODS
One gastric antral biopsy was obtained from a total of 18 untreated patients undergoing routine endoscopic evaluation of chronic abdominal complaints. Nine patients had histologic evidence of H. pylori infection and 9 age- and sex-matched patients had no histologic evidence of H. pylori infection. A microarray analysis was performed using a gene chip containing 35,000 human expressed sequence tags on RNA extracted from endoscopic, gastric antral biopsies, and average gene expression among infected and uninfected patients was compared.


RESULTS
Underexpressed genes in infected patients' mucosa included gastric intrinsic factor and several metallothionein isoforms. Overexpressed genes in infected patients' mucosa comprised MHC Class II molecules, immunoglobulin and B-cell activation genes, as well as genes known to induce apoptosis. Changes in expression were confirmed for a subset of genes by SYBR green real-time PCR.


CONCLUSIONS
Microarray analysis of antral biopsies from patients with and without H. pylori infection revealed differential expression of metal regulatory, immunity and inflammation-related genes.
Mannick
E E
EE
Louisiana State University, Stanley S Scott Cancer and Depts of Pathology and Genetics, New Orlands, Louisiana 70112, USA. manni@lsuhsc.edu
Schurr
J R
JR
Zapata
A
A
Lentz
J J
JJ
Gastanaduy
M
M
Cote
R L
RL
Delgado
A
A
Correa
P
P
Correa
H
H
D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D020869:Gene Expression Profiling; D016481:Helicobacter Infections / Q000235:genetics*; D016480:Helicobacter pylori; D006801:Humans; D007109:Immunity / Q000235:genetics; D007249:Inflammation / Q000235:genetics; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011706:Pyloric Antrum / Q000382:microbiology / Q000473:pathology; D014945:Wound Healing / Q000235:genetics
D009363:Neoplasm Proteins