Polymerase chain reaction amplification as a diagnostic tool in culture-negative multiple-valve endocarditis.
We report a case of culture-negative infectious endocarditis in a 17-year-old boy in which the etiologic diagnosis could only be provided by polymerase chain reaction amplification and sequencing of the bacterial 16S rRNA gene from valve tissue.
Madershahian
Navid
N
Department of Cardiothoracic and Vascular Surgery, Friedrich-Schiller University Jena, Jena, Germany.
Strauch
Justus T
JT
Breuer
Martin
M
Bruhin
Raimund
R
Straube
Eberhard
E
Wahlers
Thorsten
T
D000293:Adolescent; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology*; D005188:False Negative Reactions; D006349:Heart Valve Diseases / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D015994:Incidence; D008297:Male; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013290:Streptococcal Infections / Q000175:diagnosis*; D034363:Viridans Streptococci / Q000235:genetics*
D012336:RNA, Ribosomal, 16S