title: The use of high halide-ion concentrations and automated phasing procedures for the structural analysis of BclA, the major component of the exosporium of Bacillus anthracis spores.
abstract: The structure determination of the recombinant form of BclA, the major protein component of Bacillus anthracis exosporium, involved soaking in a high concentration of potassium iodide as the means of obtaining a good-quality heavy-atom derivative. The data to 2 angstroms resolution collected on a laboratory source were of sufficient quality to allow successful phasing and chain tracing by automated methods.
authors.affiliation: Institut de Génétique et Microbiologie, Bâtiment 409, Université Paris XI, 91405 Orsay, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Salamitou
authors.forename: Stéphane
authors.initials: S
authors.lastname: Réty
authors.forename: Françoise
authors.initials: F
authors.lastname: Le Hégarat
authors.forename: Gérard
authors.initials: G
authors.lastname: Leblon
authors.forename: Anita
authors.initials: A
authors.lastname: Lewit-Bentley
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D006219:Halogens / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D013171:Spores, Bacterial / Q000737:chemistry*
chemical_list: C470593:BclA protein, Bacillus anthracis; D017931:DNA Primers; D006219:Halogens; D008562:Membrane Glycoproteins