Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis.
Candida albicans PALS-green fluorescent protein (GFP) reporter strains were inoculated into mice in a disseminated candidiasis model, and GFP production was monitored by immunohistochemistry and reverse transcription-PCR (RT-PCR). GFP production from the ALS1 and ALS3 promoters was detected immunohistochemically. ALS1, ALS2, ALS3, ALS4, and ALS9 transcription was detected by RT-PCR, further identifying ALS genes expressed in this model.
Green
Clayton B
CB
Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 61802, USA.
Zhao
Xiaomin
X
Hoyer
Lois L
LL
D000373:Agglutinins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D016469:Fungemia / Q000382:microbiology*; D015870:Gene Expression; D005800:Genes, Fungal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction
C093538:ALS1 protein, Candida albicans; C113620:ALS3 protein, Candida albicans; D000373:Agglutinins; D005656:Fungal Proteins; D049452:Green Fluorescent Proteins
Microbiology (Reading). 2005 Apr;151(Pt 4):1051-1060
15817774
Curr Protoc Mol Biol. 2001 May;Chapter 13:Unit13.12
18265096
Microbiology (Reading). 2004 Feb;150(Pt 2):267-275
14766904
Arch Intern Med. 1988 Dec;148(12):2642-5
3196127
Microbiology (Reading). 1997 Feb;143 ( Pt 2):303-311
9043107
Trends Microbiol. 2001 Apr;9(4):176-80
11286882
Mol Gen Genet. 1992 Nov;235(2-3):453-7
1465116
Infect Immun. 1999 Aug;67(8):4251-5
10417199
Diagn Microbiol Infect Dis. 1998 May;31(1):327-32
9597393
Trends Microbiol. 2003 Jan;11(1):30-6
12526852
Microbiology (Reading). 2004 Jul;150(Pt 7):2415-2428
15256583
J Bacteriol. 1998 Oct;180(20):5334-43
9765564
Mol Microbiol. 2002 Apr;44(1):61-72
11967069
Yeast. 2000 Mar 15;16(4):325-7
10669870