Biosynthetic origin of the carbon skeleton and nitrogen atom of pamamycin-607, a nitrogen-containing polyketide.
The biosynthesis of pamamycin-607 (PM-607), a sixteen-membered macrodiolide compound, was studied with 13C- and 15N-labeled precursor units in Streptomyces alboniger. Feeding experiments with 13C-labeled acetate or propionate indicate that the carbon skeleton of PM-607 was derived from six acetate, four propionate and three succinate units. MS analyses of 15N-labeled PM-607 suggest that the nitrogen atom in PM-607 was derived from the alpha-amino group of an amino acid.
Hashimoto
Makoto
M
Department of Plant Protection, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Komatsu
Haruhiko
H
Kozone
Ikuko
I
Kawaide
Hiroshi
H
Abe
Hiroshi
H
Natsume
Masahiro
M
D002247:Carbon Isotopes; D018942:Macrolides / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D009587:Nitrogen Isotopes; D013302:Streptomyces / Q000378:metabolism*
D002247:Carbon Isotopes; D018942:Macrolides; D009587:Nitrogen Isotopes; C470905:pamamycin-607