title: Plant viral intergenic DNA sequence repeats with transcription enhancing activity.
abstract: BACKGROUND
  The geminivirus and nanovirus families of DNA plant viruses have proved to be a fertile source of viral genomic sequences, clearly demonstrated by the large number of sequence entries within public DNA sequence databases. Due to considerable conservation in genome organization, these viruses contain easily identifiable intergenic regions that have been found to contain multiple DNA sequence elements important to viral replication and gene regulation. As a first step in a broad screen of geminivirus and nanovirus intergenic sequences for DNA segments important in controlling viral gene expression, we have 'mined' a large set of viral intergenic regions for transcriptional enhancers. Viral sequences that are found to act as enhancers of transcription in plants are likely to contribute to viral gene activity during infection.
  
  
  RESULTS
  DNA sequences from the intergenic regions of 29 geminiviruses or nanoviruses were scanned for repeated sequence elements to be tested for transcription enhancing activity. 105 elements were identified and placed immediately upstream from a minimal plant-functional promoter fused to an intron-containing luciferase reporter gene. Transient luciferase activity was measured within Agrobacteria-infused Nicotiana tobacum leaf tissue. Of the 105 elements tested, 14 were found to reproducibly elevate reporter gene activity (>25% increase over that from the minimal promoter-reporter construct, p < 0.05), while 91 elements failed to increase luciferase activity. A previously described "conserved late element" (CLE) was identified within tested repeats from 5 different viral species was found to have intrinsic enhancer activity in the absence of viral gene products. The remaining 9 active elements have not been previously demonstrated to act as functional promoter components.
  
  
  CONCLUSIONS
  Biological significance for the active DNA elements identified is supported by repeated isolation of a previously defined viral element (CLE), and the finding that two of three viral enhancer elements examined were markedly enriched within both geminivirus sequences and within Arabidopsis promoter regions. These data provide a useful starting point for virologists interested in undertaking more detailed analysis of geminiviral promoter function.
authors.affiliation: USDA-ARS, Plant Stress and Water Conservation Laboratory, 3810 4th St,, Lubbock, TX 79415, USA. jvelten@lbk.ars.usda.gov
authors.forename: Jeff
authors.initials: J
authors.lastname: Velten
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Morey
authors.forename: Christopher I
authors.initials: CI
authors.lastname: Cazzonelli
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D021901:DNA, Intergenic / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D017343:Genes, Plant; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014026:Nicotiana; D014158:Transcription, Genetic
chemical_list: D021901:DNA, Intergenic; D004279:DNA, Viral
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