title: Functional and biochemical modifications in skeletal muscles from malarial mice.
abstract: Although it is well established that patients suffering from malaria experience skeletal muscle problems (contracture, aches, fatigue, weakness), detailed studies have not been performed to investigate changes in the contractile function and biochemical properties of intact and skinned skeletal muscles of mammals infected with malaria. To this end, we investigated such features in the extensor digitorium longus (EDL, fast-twitch, glyocolytic) and in the soleus (SOL, slow-twitch, oxidative) muscles from mice infected with Plasmodium berghei. We first studied maximal tetanic force (T(max)) produced by intact control and malaria-infected muscles before, during and after fatigue. Triton-skinned muscle fibres were isolated from these muscles and used to determine isometric contractile features as well as a basic biochemical profile as analysed by silver-enhanced SDS-PAGE. We found that the T(max) of intact muscles and the maximal Ca2+-activated force (F(max)) of Triton-skinned muscle fibres were reduced by approximately 50% in malarial muscles. In addition, the contractile proteins of Triton-skinned muscle fibres from malarial muscles were significantly less sensitive to Ca2+. Biochemical analysis revealed that there was a significant loss of essential contractile proteins (e.g. troponins and myosin) in Triton-skinned muscle fibres from malarial muscles as compared to controls. The biochemical alterations (i.e., reduction of essential contractile proteins) seem to explain well the functional modifications resolved in both intact muscles and Triton-skinned muscle fibres and may provide a suitable paradigm for the aetiology of muscle symptoms associated with malaria.
authors.affiliation: Department of Physiology and Biophysics, University of Medicine & Dentistry of New Jersey-Robert Wood Johnson School of Medicine, Piscataway, NJ 08854, USA. brottoma@umdnj.edu
authors.forename: Marco A P
authors.initials: MA
authors.lastname: Brotto
authors.forename: Mauro T
authors.initials: MT
authors.lastname: Marrelli
authors.forename: Leticia S
authors.initials: LS
authors.lastname: Brotto
authors.forename: Marcelo
authors.initials: M
authors.lastname: Jacobs-Lorena
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Nosek
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007537:Isometric Contraction; D008288:Malaria / Q000503:physiopathology*; D008297:Male; D051379:Mice; D018763:Muscle Fatigue; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000473:pathology / Q000503:physiopathology*
chemical_list: D009124:Muscle Proteins