title: Biochemical changes as enzymatic defense system of phenanthrene exposure in olive flounder, Paralichthys olivaceus.
abstract: The objective of this study was to investigate early biological response in olive flounder exposed to sub-lethal concentrations of waterborne phenanthrene (0.5, 1 or 2 microM). The fish were exposed for 4 weeks and we analyzed their enzymatic defense system, antioxidant and phase II enzyme activities, to evaluate the chronic exposure toxicity of phenanthrene. Waterborne phenanthrene affected antioxidant enzymes and glutathione-mediated detoxification as enzyme defense system. Hepatic, gill and kidney glutathione reductase as well as glutathione S-transferase, and catalase activities were markedly elevated after two or four weeks of exposure. These enzymes activities of olive flounder, Paralichthys olivaceus seem to be a convenient tool for monitoring pollution in coastal areas against PAHs pollution including phenanthrene.
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Korea .
authors.forename: J-H
authors.initials: JH
authors.lastname: Jee
authors.forename: J-C
authors.initials: JC
authors.lastname: Kang
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D005432:Flounder / Q000378:metabolism*; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008658:Inactivation, Metabolic; D007564:Japan; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D007723:Korea; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D010616:Phenanthrenes / Q000493:pharmacokinetics*; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*
chemical_list: D010616:Phenanthrenes; D014874:Water Pollutants, Chemical; D002374:Catalase; D005980:Glutathione Reductase; D005982:Glutathione Transferase