title: In-vitro dual binding activity of a evolutionarily related subgroup of hnRNP proteins.
abstract: The wide family of heterogeneous nuclear ribonucleoproteins (hnRNPs) comprises members that interact with single-stranded nucleic acids. On the basis of their structure, some of them are characterised by a tandem RNA-binding domain (RBD) and a glycine-rich C-terminus, showing a high degree of homology. Recently, we have isolated some proteins belonging to this group that interact with single-stranded cytosine-block telomeric DNA. The aim of the present investigation is to better characterise the relationship of some structural features shared by these proteins and their in-vitro interaction with the telomeric type sequences. We analysed the in-vitro binding properties of some of these components toward both single-stranded telomeric motifs. Using deletion mutants, the relationship between cytosine-rich motif binding activity and the structural features of one of these proteins is further characterized. This binding activity appears to be related to a subgroup of the 2xRBD+Glycine rich hnRNP, suggesting functionally distinct properties of these proteins, in agreement with their evolutionary relationship.
authors.affiliation: Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, 34127 Trieste, Italy. bandiera@bbcm.univ.trieste.it
authors.forename: Antonella
authors.initials: A
authors.lastname: Bandiera
authors.forename: Nevenka
authors.initials: N
authors.lastname: Medic
authors.forename: A Akintunde
authors.initials: AA
authors.lastname: Akindahunsi
authors.forename: Giorgio
authors.initials: G
authors.lastname: Manzini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence / Q000235:genetics / Q000502:physiology; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D040681:Structural Homology, Protein; D016615:Telomere / Q000235:genetics / Q000378:metabolism*
chemical_list: D034502:Heterogeneous-Nuclear Ribonucleoprotein D; D022661:RNA, Untranslated
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