[Effect of sensory neuropeptide substance P on the differentiation of cultured epidermal stem cells in vitro].
OBJECTIVE
To investigate the effect of sensory neuropeptide substance P (SP) on the differentiation of cultured epidermal stem cells (ESC) in vitro,with in vitro cultured ESC as the platform.


METHODS
ESC from newborn Wistar rats were isolated, purified by repeated passages in culture. SP was added for stimulation when ESC clone grew. Immunohistochemistry staining with K14 antibody, and flow cytometry (FCM) was performed at 0, 24th, 48th, 72nd, 96th, 144th, 192nd, 240th, 288th, 336th, 384th, 432nd post differentiation hours (PDH) to identify the cell groups and to detect if there were transient amplifying cells (TAC) among the cells.


RESULTS
ESC in culture formed large colonies after SP treatment with positive staining for K14, indicating that they were TACs. The results of FCM indicated that when ESC were stimulated by SP, TAC colony formation occurred and the cell number increased in a constant speed.


CONCLUSIONS
ESC could differentiate into TAC by neuropeptide SP induction, and the number of ESC kept on a certain level during the process.
Huang
Hui
H
Institute of Field Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, P.R. China.
Lai
Xi-nan
XN
Wang
Zheng-guo
ZG
Wang
Li-li
LL
Chen
Zhi-xiong
ZX
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology*; D013373:Substance P / Q000494:pharmacology*
D013373:Substance P