title: Diethyldithiocarbamate inhibits iNOS expression in human lens epithelial cells stimulated by IFN-gamma and LPS.
abstract: OBJECTIVE
  To investigate the biological activity of human lens epithelial cells (HLEC) in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and to assesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels and expression of NOS.
  
  
  METHODS
  The human lens epithelial cell line SRA 01/04 was used in this experiment. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect, respectively, iNOS mRNA expression and protein production.
  
  
  RESULTS
  A costimulation by interferon gamma (IFN-gamma) and lipopolysaccharide (LPS) was necessary for iNOS expression in HLEC. The expression of iNOS was significantly reduced in a dose-dependent manner by adding DDC from 10 micromol/L to 1 mmol/L.
  
  
  CONCLUSIONS
  The expression of iNOS in HLEC needs co-stimulation with IFN-gamma and LPS and it is inhibited by DDC.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: De-xin
authors.initials: DX
authors.lastname: Li
authors.forename: Si-ling
authors.initials: SL
authors.lastname: Wang
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Ito
authors.forename: Jian-hai
authors.initials: JH
authors.lastname: Zhang
authors.forename: Chun-fu
authors.initials: CF
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D004050:Ditiocarb / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000201:enzymology; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology / Q000201:enzymology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D004050:Ditiocarb; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II