The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetate.
The aim of the present investigation was to elucidate whether the Golgi apparatus undergoes photodamage following administration of the fluorogenic substrates Rose Bengal acetate (RBAc) and irradiation at the appropriate wavelength. Human HeLa cells were treated in culture and the changes in the organization of the Golgi apparatus were studied using fluorescence confocal microscopy and electron microscopy, after immunocytochemical labeling. To see whether the cytoskeletal components primarily involved in vesicle traffic (i.e., microtubules) might also be affected, experiments of tubulin immunolabeling were performed. After treatment with RBAc and irradiation, cells were allowed to grow in drug-free medium for different times. 24 hr after irradiation, the cisternae of the Golgi apparatus became packed, and after 48-72 hr they appeared more fragmented and scattered throughout the cytoplasm; these changes in the organization of the Golgi cisternae were confirmed at electron microscopy. Interestingly enough, apoptosis was found to occur especially 48-72 h after irradiation, and apoptotic cells exhibited a dramatic fragmentation of the Golgi membranes. The immunolabeling with anti-tubulin antibody showed that microtubules were also affected by irradiation in RBAc-treated cells.
Soldani
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Dipartimento di Biologia Animale, Università degli Studi di Pavia, Piazza Botta 10, 27100 Pavia, Italy.
Bottone
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MG
Croce
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AC
Fraschini
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A
Bottiroli
G
G
Pellicciari
C
C
D005456:Fluorescent Dyes / Q000633:toxicity; D006056:Golgi Apparatus / Q000187:drug effects* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D046790:Pancreas, Exocrine / Q000648:ultrastructure; D017319:Photosensitizing Agents / Q000633:toxicity*; D012395:Rose Bengal / Q000031:analogs & derivatives* / Q000633:toxicity*
D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; C501991:Rose Bengal acetate; D012395:Rose Bengal