[Effects of Epimedium pubescens icariine on proliferation and differentiation of human osteoblasts].
OBJECTIVE
To study the effects of epimedium pubescens icariine on the proliferation and differentiation of human osteoblasts.


METHODS
Human osteoblasts were obtained by inducting human marrow mesenchymal stem cells (hMSCs) directionally. MTT was used to observe the proliferation and activity of ALP was assayed to observe the differentiation of the third passage human osteoblasts cultured in vitro. The expression of BMP-2 mRNA was checked by RT-PCR.


RESULTS
Epimedium pubescens icariine at the dose of 20 microg x mL(-1) increased greatly the proliferation and differentiation of human osteoblasts and promoted the expression of BMP-2 mRNA.


CONCLUSIONS
Epimedium pubescens icariine enhances significantly the proliferation and differentiation of human osteoblasts, which may be mediated by increasing the expression of BMP-2 mRNA.
Yin
Xiao-xue
XX
Third Hospital of Peking University, Beijing 100083, China. yxx2733_cn@sina.com
Chen
Zhong-qiang
ZQ
Dang
Geng-ting
GT
Ma
Qing-jun
QJ
Liu
Zhong-jun
ZJ
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D005419:Flavonoids; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C056599:icariin