title: Antimicrobial resistance and beta-lactamase production of clinical isolates of prevotella and porphyromonas species.
abstract: BACKGROUND
  This study determined the beta-lactamase production and the antimicrobial resistance of 72 Prevotella species and 48 Porphyromonas species isolated from different clinical specimens.
  
  
  METHODS
  All strains were identified using API 32 ID. The beta-lactamase production was determined by nitrocefin disks. E test strips of benzylpenicillin, ampicillin + sulbactam, cefoxitin, clindamycin, metronidazole and imipenem were tested for each strain.
  
  
  RESULTS
  Nineteen Prevotella melaninogenica, 18 Prevotella intermedia, 16 Prevotella denticola, 11 Prevotella loescheii and 8 Prevotella bivia strains were identified. Four were clindamycin resistant. The highest beta-lactamase production was found at a rate of 68.4% in P. melaninogenica species. Additionally, 33 Porphyromonas asaccharolytica and 15 Porphyromonas gingivalis strains were identified. None of them produced beta-lactamase.
  
  
  CONCLUSIONS
  In view of the emerging antibiotic resistance among anaerobes, the current local susceptibility profile of our Prevotella and Porphyromonas species will establish the basis for additional surveys tracing significant changes in the antimicrobial resistance of our clinical isolates.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Cerrahpaşa School of Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Hrisi
authors.initials: H
authors.lastname: Bahar
authors.forename: Muzeyyen Mamal
authors.initials: MM
authors.lastname: Torun
authors.forename: Mehmet
authors.initials: M
authors.lastname: Demirci
authors.forename: Bekir
authors.initials: B
authors.lastname: Kocazeybek
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D017861:Porphyromonas / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D018720:Prevotella / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D011446:Prospective Studies; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases