Liposomal polyene antibiotics.
Polyene antibiotics (i.e., amphotericin B and nystatin) have been incorporated into lipid-based delivery systems to decrease their toxicity and enhance their therapeutic index, the most common being liposomes. This chapter describes the protocols for preparing liposomal amphotericin B and determining the efficacy and toxicity of the formulations in animals. Furthermore, methods for determining the pharmacokinetics and drug distribution after administration of amphotericin B in lipid-based delivery systems are discussed. Procedures for comparing the toxicity of different amphotericin B formulations in cell culture studies are also elucidated.
Ng
Agatha W K
AW
Division of Pharmaceutics and Biopharmaceutics, The University of British Columbia, Vancouver, British Columbia, Canada.
Wasan
Kishor M
KM
Lopez-Berestein
Gabriel
G
D000666:Amphotericin B / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000633:toxicity; D002177:Candidiasis / Q000188:drug therapy; D002460:Cell Line; D004337:Drug Carriers; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D009761:Nystatin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000633:toxicity; D011817:Rabbits
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008081:Liposomes; D009761:Nystatin; D000666:Amphotericin B