title: Profile of Candida albicans-secreted aspartic proteinase elicited during vaginal infection.
abstract: Vaginal infections caused by the opportunistic yeast Candida albicans are a significant problem in women of child-bearing age. Several factors are recognized as playing a crucial role in the pathogenesis of superficial candidiasis; these factors include hyphal formation, phenotypic switching, and the expression of virulence factors, including a 10-member family of secreted aspartic proteinases. In the present investigation, we analyzed the secreted aspartic proteinase gene (SAP) expression profile of C. albicans that is elicited in the course of vaginal infection in mice and how this in vivo expression profile is associated with hyphal formation. We utilized two different genetic reporter systems that allowed us to observe SAP expression on a single-cell basis, a recombination-based in vivo expression technology and green fluorescent protein-expressing Candida reporter strains. Of the six SAP genes that were analyzed (SAP1 to SAP6), only SAP4 and SAP5 were detectably induced during infection in this model. Expression of both of these genes was associated with hyphal growth, although not all hyphal cells detectably expressed SAP4 and SAP5. SAP5 expression was induced soon after infection, whereas SAP4 was expressed at later times and in fewer cells compared with SAP5. These findings point to a link between morphogenetic development and expression of virulence genes during Candida vaginitis in mice, where host signals induce both hyphal formation and expression of SAP4 and SAP5, but temporal gene expression patterns are ultimately controlled by other factors.
authors.affiliation: Institute of Clinical Microbiology, Immunology, and Hygiene, Friedrich-Alexander Universität Erlangen-Nürnberg, Wasserturmstrasse 3/5, D-91054 Erlangen, Germany.
authors.forename: Brad N
authors.initials: BN
authors.lastname: Taylor
authors.forename: Peter
authors.initials: P
authors.lastname: Staib
authors.forename: Ayfer
authors.initials: A
authors.lastname: Binder
authors.forename: Antje
authors.initials: A
authors.lastname: Biesemeier
authors.forename: Miriam
authors.initials: M
authors.lastname: Sehnal
authors.forename: Martin
authors.initials: M
authors.lastname: Röllinghoff
authors.forename: Joachim
authors.initials: J
authors.lastname: Morschhäuser
authors.forename: Klaus
authors.initials: K
authors.lastname: Schröppel
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism*; D002176:Candida albicans / Q000201:enzymology / Q000254:growth & development / Q000472:pathogenicity*; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology / Q000503:physiopathology*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D025301:Hyphae / Q000201:enzymology / Q000254:growth & development; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D005656:Fungal Proteins; D016282:Aspartic Acid Endopeptidases; C086392:SAP4 protein, Candida albicans; C090755:SAP5 protein, Candida albicans
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