A serological investigation into contagious caprine pleuropneumonia (CCPP) in Ethiopia.
For a comparison of serological tests for CCPP, sera from 767 goats were examined. They were subjected to three tests: complement fixation test (CFT) with Mycoplasma capricolum subspecies capripneumoniae antigen; blocking ELISA (B-ELISA) with Mycoplasma capricolum subspecies capripneumoniae antigen; and CFT with Mycoplasma mycoides subspecies mycoides small colony type antigen. Antibodies were detected by these three tests in 23%, 2% and 12%, respectively, of sera from districts in which CCPP had not been reported, and in 60%, 83% and 87%, respectively, in sera from areas in which CCPP had been reported. The specificity of the tests is discussed. The use of the B-ELISA test for the diagnosis and for epidemiological studies of CCPP is strongly recommended.
Sharew
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AD
Ministry of Agriculture, National Veterinary Institute, Debre Zeit, Ethiopia. dhwp.vet.med@telecom.net.et
Staak
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Thiaucourt
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Roger
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D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005002:Ethiopia / Q000453:epidemiology; D015511:Goat Diseases / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology*; D006041:Goats; D009174:Mycoplasma / Q000276:immunology* / Q000302:isolation & purification; D011002:Pleuropneumonia, Contagious / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology*; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
J Vet Diagn Invest. 1994 Oct;6(4):428-34
7858022
Vet Rec. 1990 Oct 6;127(14):353
2260241
Vet Microbiol. 2001 Sep 3;82(1):27-37
11423192
Int J Syst Bacteriol. 1993 Jul;43(3):597-602
8347516
Zentralbl Bakteriol Orig A. 1979 Oct;245(1-2):164-70
44610
Vet Microbiol. 2000 Mar 15;72(3-4):251-68
10727835
Trop Anim Health Prod. 1989 Feb;21(1):43-9
2711463
Res Vet Sci. 1987 Mar;42(2):175-8
3589164
Vet Rec. 1992 Dec 19-26;131(25-26):585
1287955
Vet Microbiol. 1994 Aug 1;41(3):191-203
7975145