title: Characterization and functional investigation of an Arabidopsis cDNA encoding a homologue to the d-PGMase superfamily.
abstract: An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase superfamily: cofactor-dependent phosphoglycerate mutases (d-PGM-ases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Preliminary phylogenetic studies indicated that At-74 cDNA and its close homologue in Arabidopsis, At-74H, belong, however, to an equally distinct group. At-74 was ubiquitously expressed in vegetative organs and induced by glucose. The At-74 cDNA was overexpressed in A. thaliana to investigate its function, but this overexpression did not result in a clear phenotype. Enzymatic assays performed on At-74-overproducing transgenic plants or E. coli cells showed no increase in either the activities of cofactor-dependent and -independent phosphoglycerate mutases (i-PGMases) and F2,6Pase or that of acid phosphatases. The possible role of At-74 in plant metabolism was further investigated by carbon partitioning experiments with [U-(14)C] glucose and measurements of soluble sugars in both young leaves and roots. Two overexpressing At-74 lines showed a clear increase in glucose uptake. This paper introduces the At-74 homologue of the d-PGMase superfamily members and supports a possible role of At-74 in carbohydrate metabolism.
authors.affiliation: Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, CP 242, Bd du Triomphe, B-1050 Bruxelles, Belgium.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Bourgis
authors.forename: Fredrik C
authors.initials: FC
authors.lastname: Botha
authors.forename: Srikrishnan
authors.initials: S
authors.lastname: Mani
authors.forename: Fletcher N
authors.initials: FN
authors.lastname: Hiten
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Rigden
authors.forename: Nathalie
authors.initials: N
authors.lastname: Verbruggen
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D002250:Carbon Radioisotopes; D016384:Consensus Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D025221:Phosphofructokinases / Q000235:genetics; D010736:Phosphoglycerate Mutase / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D002250:Carbon Radioisotopes; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D025221:Phosphofructokinases; D010736:Phosphoglycerate Mutase