title: Optimum dose of LH-RH analogue Fertirelin Acetate for the induction of superovulation in mice.
abstract: The optimum dose for establishing superovulation in mice of Fertirelin Acetate (FA), an LH-RH analogue, was examined. Mice were subcutaneously injected with 5 IU of hCG at 17:00 (Day 0), and with various doses of FA (0.001 to 1.0 microg) five times at 4 h intervals on and after 22:00 on Day 0. To induce ovulation, 5 IU of hCG was again injected subcutaneously at 17:00 on Day 2. In the groups administered with doses ranging from 0.01 to 0.5 microg of FA, the number of ovulated eggs was significantly (p<0.05) larger than in the control group (12.9 +/- 5.9). The greatest number of ovulated eggs (22.6 +/- 7.3) was obtained in the group administered with 0.025 microg of FA. The results indicate that the effective dose of LH-RH analogue, FA, is between 0.1 and 0.5 microg for superovulation induction in mice.
authors.affiliation: Laboratory Animal Facilities, Research Center for Medical Sciences, The Jikei University School of Medicine, Kashiwa, Chiba, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Nariai
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ishinazaka
authors.forename: Kazuyosi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchiyama
authors.forename: Kahei
authors.initials: K
authors.lastname: Sato
authors.forename: Ryuji
authors.initials: R
authors.lastname: Asano
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Tsumagari
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Yukawa
authors.forename: Kiichi
authors.initials: K
authors.lastname: Kanayama
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D010062:Ovulation Induction / Q000379:methods*; D013480:Superovulation / Q000187:drug effects
chemical_list: D007987:Gonadotropin-Releasing Hormone; C009936:fertirelin