Molecular cloning and sequence analysis of full-length growth hormone cDNAs from six important economic fishes.
In this study,the full-length cDNAs of GH (Growth Hormone) gene was isolated from six important economic fishes, Siniperca kneri, Epinephelus coioides, Monopterus albus, Silurus asotus, Misgurnus anguillicaudatus and Carassius auratus gibelio Bloch. It is the first time to clone these GH sequences except E. coioides GH. The lengths of the above cDNAs are as follows: 953 bp, 1 023 bp, 825 bp, 1 082 bp, 1 154 bp and 1 180 bp. Each sequence includes an ORF of about 600 bp which encodes a protein of about 200 amino acid: S. kneri, E. coioides and M. albus GHs of 204 amino acid, S. asotus GH of 200 amino acid, M. anguillicaudatus and C. auratus gibelio GHs of 210 amino acid. Then detailed sequence analysis of the six GHs with many other fish sequences was performed. The six sequences all showed high homology to other sequences, especially to sequences within the same order, and many conserved residues were identified, most localized in five domains. The phylogenetic trees (MP and NJ) of many fish GH ORF sequences (including the new six) with Amia calva as outgroup were generally resolved and largely congruent with the morphology-based tree though some incongruities were observed, suggesting GH ORF should be paid more attention to in teleostean phylogeny.
Zhang
Jing-Nan
JN
Key Laboratory of the Ministry of Education for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China. elizabeth.z@163.com
Song
Ping
P
Hu
Jia-Rui
JR
Mo
Sai-Jun
SJ
Peng
Mao-Yu
MY
Zhou
Wei
W
Zou
Ji-Xing
JX
Hu
Yin-Chang
YC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics; D003001:Cloning, Molecular; D003531:Cypriniformes / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis*; D005399:Fishes / Q000235:genetics*; D006054:Goldfish / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010473:Perciformes / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D013006:Growth Hormone