[Determination of triptolide in Tripterygium total terpenoids tablets by HPLC].
OBJECTIVE
To develop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets.


METHODS
A Lichrospher CN column was used with ethanol and water for gradient elution. The detection wavelength was set at 255 nm.


RESULTS
The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 microg x mL(-1) (r = 0.9998). The average recovery was 99.2%, RSD% = 1.7%.


CONCLUSIONS
The method is simple, rapid and accurate and can be used for quality control of the tablets.
Pu
Cun-hai
CH
Nanjing Zhongshan Pharmaceutical Factory, Nanjing 210012, China.
He
Ming-fang
MF
Zhanag
Xiao-qian
XQ
Shen
Jia
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004224:Diterpenes / Q000032:analysis*; D004852:Epoxy Compounds; D010616:Phenanthrenes / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013607:Tablets; D013729:Terpenes / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D029757:Tripterygium / Q000737:chemistry*
D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; D013607:Tablets; D013729:Terpenes; C001899:triptolide