title: Application of functional genomic technologies in a mouse model of retinal degeneration.
abstract: Generation of tissue-specific, normalized and subtracted cDNA libraries has the potential to characterize the expression of rare transcriptional units not represented on Affymetrix GeneChips. Initial sequence analysis of our murine cDNA clone collections showed that as much as 86, 45, and 30% of clones are not represented on the Affymetrix Mu11k, MG-U74, and MG-430 chip sets, respectively. A detailed study that compared EST sequences of a subtracted library generated from mouse retina to those of MG-430 consensus sequences was undertaken, using UniGene build 124 as the common reference. A set of 1111 nonredundant transcript regions, not represented on the commercial array, was identified. These clusters were used as the primary filter for analyzing a data set produced by assaying samples from the Pde6b(rd1) mouse model of retinal degeneration on a 12,325-feature retinal cDNA microarray. QRT-PCR validated eight unique transcripts identified by microarray. Seven of the transcripts showed retina-specific expression. Full-length cloning strategies were applied to two of the ESTs. The genes discovered by this approach are the full-length mouse homologue of guanylate cyclase 2F (GUCY2F) and a carboxy-truncated splice variant of retinal S-antigen (SAG), known as regulators of the visual phototransduction G-protein-coupled receptor-mediated signaling pathway. These sequences have been assigned GenBank Accession Nos. and , respectively.
authors.affiliation: Research Molecular Discovery, Biogen Idec, Inc., 14 Cambridge Center, Cambridge, MA 02142, USA. jeff.shearstone@biogenidec.com
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Shearstone
authors.forename: Yang E
authors.initials: YE
authors.lastname: Wang
authors.forename: Amanda
authors.initials: A
authors.lastname: Clement
authors.forename: Normand E
authors.initials: NE
authors.lastname: Allaire
authors.forename: Chunhua
authors.initials: C
authors.lastname: Yang
authors.forename: Dane S
authors.initials: DS
authors.lastname: Worley
authors.forename: John P
authors.initials: JP
authors.lastname: Carulli
authors.forename: Steven
authors.initials: S
authors.lastname: Perrin
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D004195:Disease Models, Animal; D023281:Genomics; D006162:Guanylate Cyclase / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D012162:Retinal Degeneration / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D006162:Guanylate Cyclase; C484783:Gucy2f protein, mouse