title: Isolation of Delta9-THCA-A from hemp and analytical aspects concerning the determination of Delta9-THC in cannabis products.
abstract: A simple procedure based on a common silica gel column chromatography for the isolation of Delta9-tetrahydrocannabinolic acid A (Delta9-THCA-A) from hemp in a multi-milligram scale is presented. Further, the decarboxylation reaction of Delta9-THCA-A to the toxicologically active Delta9-tetrahydrocannabinol (Delta9-THC) at different analytical and under-smoking conditions is investigated. Maximal conversion in an optimised analytical equipment yields about 70% Delta9-THC. In the simulation of the smoking process, only about 30 % of the spiked substance could be recovered as Delta9-THC.
authors.affiliation: Institute of Legal Medicine, Pestalozzistrasse 22, CH-4056 Basel, Switzerland. franz.dussy@bs.ch
authors.forename: Franz E
authors.initials: FE
authors.lastname: Dussy
authors.forename: Cornelia
authors.initials: C
authors.lastname: Hamberg
authors.forename: Marco
authors.initials: M
authors.lastname: Luginbühl
authors.forename: Thomas
authors.initials: T
authors.lastname: Schwerzmann
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Briellmann