title: A rare fraction of human hematopoietic stem cells with large telomeres.
abstract: The lack of specific markers for stem cells makes the physical identification of this compartment difficult. Hematopoietic stem cells differ in their repopulating and self-renewal potential. Our study shows that multiple classes of human hematopoietic CD34+ greatly differ in telomere length. Flow-cytometry-based fluorescent in situ hybridization and confocal microscopy of CD34+ cells has revealed remarkable telomere length heterogeneity, with a hybridization pattern consistent with different classes of human hematopoietic progenitor cells. These results also point to the existence of a significant clonal heterogeneity among primitive hematopoietic cells and provide the first evidence of a rare fraction of CD34+ cells with large telomeres in humans.
authors.affiliation: Cryopreservation Unit, Hospital Clínic, Institut dInvestigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: García-Escarp
authors.forename: Vanessa
authors.initials: V
authors.lastname: Martinez-Muñoz
authors.forename: Jordi
authors.initials: J
authors.lastname: Barquinero
authors.forename: Irene
authors.initials: I
authors.lastname: Sales-Pardo
authors.forename: Joan Carles
authors.initials: JC
authors.lastname: Domingo
authors.forename: Pedro
authors.initials: P
authors.lastname: Marin
authors.forename: Jordi
authors.initials: J
authors.lastname: Petriz
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D002460:Cell Line; D002469:Cell Separation; D002999:Clone Cells; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018613:Microscopy, Confocal; D016615:Telomere / Q000502:physiology / Q000648:ultrastructure*
chemical_list: D018952:Antigens, CD34