title: Effects of different anti-tau antibodies on tau fibrillogenesis: RTA-1 and RTA-2 counteract tau aggregation.
abstract: Tau is the major antigenic component of neurofibrillary pathology in tauopathy, including Alzheimer's disease. Although conversion of soluble tau to an insoluble polymerized fibrillar form is a key factor in the pathogenesis of tauopathy, the mechanism of the change is unclear and no inhibitors of fibril formation are available. Monoclonal antibodies against the 1st or 2nd repeat of the microtubule binding domain, but not the C-terminal 16 residues, completely inhibited tau aggregation into PHF. Furthermore, they did not inhibit tau-induced tubulin assembly. Thus, they are useful to investigate tau protein conversion and will be useful therapeutic lead materials.
authors.affiliation: Biosignal Research Center, Kobe University, Kobe, Hyogo 657-8501, Japan. tanigu@kobe-u.ac.jp
authors.forename: Taizo
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Miho
authors.initials: M
authors.lastname: Sumida
authors.forename: Shuko
authors.initials: S
authors.lastname: Hiraoka
authors.forename: Koji
authors.initials: K
authors.lastname: Tomoo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kakehi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Minoura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Koji
authors.initials: K
authors.lastname: Inaka
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Ishida
authors.forename: Naoaki
authors.initials: N
authors.lastname: Saito
authors.forename: Chikako
authors.initials: C
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D016466:CHO Cells; D006224:Cricetinae; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D020836:Protein Structure, Quaternary / Q000187:drug effects; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism; D016875:tau Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D014404:Tubulin; D016875:tau Proteins