Effects of exogenous ubiquitin on cell division cycle mutants of Schizosaccharomyces pombe.
Many important cellular processes like cell cycle are regulated by selective degradation of short-lived cellular proteins via the ubiquitin-proteasome pathway. Deregulation in degradation of any of these controlling molecules can lead to abnormalities like malignancies, neurodegenerative disorders, etc. Research on effects of exogenously added Ubiquitin (Ub) on cell cycle has been lacking. This report describes the effects of exogenously added Ub on the growth of Schizosaccharomyces pombe cells. Addition of Ub was found to cause inhibition in growth of cells. In temperature sensitive cell division cycle mutant, which exhibits arrest at the G2 phase, the exogenously added Ub affected the cell-cycle arrest. Addition of Lactacystin, an inhibitor of the proteasome degradation pathway, abolished the effects of externally added Ub. A proposal has been made on the mechanism through which externally added Ub may exert its effects on cells.
Kutty
Binoj C
BC
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
Pasupathy
K
K
Mishra
K P
KP
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D005800:Genes, Fungal; D009154:Mutation; D061988:Proteasome Inhibitors; D012568:Schizosaccharomyces / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics; D025801:Ubiquitin / Q000494:pharmacology*
D061988:Proteasome Inhibitors; D025801:Ubiquitin; C067713:lactacystin; D000111:Acetylcysteine