title: [Study on the interactions between Ligusticum chuanxiong extract and cardiac muscle membrane receptors by CMSP chromatography].
abstract: OBJECTIVE
  To study the interactions between Ligusticum chuanxiong Hort extract and cardiac muscle membrane receptors.
  
  
  METHODS
  The cell membrane of rabbit cardiac muscle was fixed on silicon to make cell membrane stationary phase (CMSP), and then the interactions were studied by comparing the retention characteristics of the extracts from different solvents with those of the antagonists or activators corresponding to known receptors in cardiac muscle membrane, and by competition effect on the retention characteristics of extracts when adding the antagonists or activators into the mobile phase.
  
  
  RESULTS
  Water extract and ethanol extract both had retentions on CMSP; the retention characteristics of water extract could be affected when water extract was in competition with the antagonists for alpha receptor, and could not be affected when with the activator beta1 receptor.
  
  
  CONCLUSIONS
  It is possible that some components in water extract may combine with alpha receptor and no component with beta1 receptor, and that some components in ethanol extract may combine with cardiac muscle cell membrane. The process between active components and receptors in vivo can be imitated through the interactions between drugs and CMSP. The method provides references for the resolution of two applications: to screen the active components from Chinese medicine, and to figure out the type of receptors involved.
authors.affiliation: Key Laboratory of Medicinal Plant Resources and Nature Pharmaceutical Chemistry (Ministry of Education), Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Xuan-feng
authors.initials: XF
authors.lastname: Yue
authors.forename: Yan-ni
authors.initials: YN
authors.lastname: Zhang
authors.forename: Zhi-qi
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Zhen-jun
authors.initials: ZJ
authors.lastname: Tian
authors.forename: Jian-xiong
authors.initials: JX
authors.lastname: Yang
authors.forename: Fa-rong
authors.initials: FR
authors.lastname: Li
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000378:metabolism; D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000378:metabolism; D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D030004:Ligusticum / Q000737:chemistry*; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry*; D011485:Protein Binding; D011817:Rabbits; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism
chemical_list: D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D004365:Drugs, Chinese Herbal; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta