title: In vitro growth of human urinary tract smooth muscle cells on laminin and collagen type I-coated membranes under static and dynamic conditions.
abstract: This study investigates in vitro growth of human urinary tract smooth muscle cells under static conditions and mechanical stimulation. The cells were cultured on collagen type I- and laminin-coated silicon membranes. Using a Flexcell device for mechanical stimulation, a cyclic strain of 0-20% was applied in a strain-stress-time model (stretch, 104 min relaxation, 15 s), imitating physiological bladder filling and voiding. Cell proliferation and alpha-actin, calponin, and caldesmon phenotype marker expression were analyzed. Nonstretched cells showed significant better growth on laminin during the first 8 days, thereafter becoming comparable to cells grown on collagen type I. Cyclic strain significantly reduced cell growth on both surfaces; however, better growth was observed on laminin. Neither the type of surface nor mechanical stimulation influenced the expression pattern of phenotype markers; alpha-actin was predominantly expressed. Coating with the extracellular matrix protein laminin improved in vitro growth of human urinary tract smooth muscle cells.
authors.affiliation: Laboratory of Experimental Pediatric Urology, Department of Pediatric Surgery, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hubschmid
authors.forename: Phaik-Mooi
authors.initials: PM
authors.lastname: Leong-Morgenthaler
authors.forename: Aurelia
authors.initials: A
authors.lastname: Basset-Dardare
authors.forename: Sylvie
authors.initials: S
authors.lastname: Ruault
authors.forename: Peter
authors.initials: P
authors.lastname: Frey
mesh_terms: D015415:Biomarkers / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007223:Infant; D007797:Laminin / Q000494:pharmacology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D013314:Stress, Mechanical; D001743:Urinary Bladder / Q000166:cytology; D014551:Urinary Tract / Q000166:cytology*
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D007797:Laminin