title: A method for the determination of the residual chloroform in defatted cancellous bone transplants.
abstract: The removal of fat from cancellous bone tissue promotes the clinical healing of the transplant and improves the penetration of chemical sterilisation media into the tissue. Treatment using chloroform/methanol (2:1, 2 h) is frequently used as a defatting procedure. Eight rinses with methanol followed by two rinses with aqua ad iniectabilia (20 min each, with ultrasonic effect) ensure depletion in the level of chloroform from defatted cancellous bone to a concentration below 25 ppm (limit value). For the necessary routine quality checks on the production process, a gas chromatography method has been developed that determines the level of chloroform in cancellous bone, for which the detection limit is 0.003 ppm.
authors.affiliation: Institute for Transfusion Medicine (Tissue Bank), University Hospital Charité, Berlin, Germany. axel.pruss@charite.de
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kalus
authors.forename: Helga
authors.initials: H
authors.lastname: Müller
authors.forename: Hans
authors.initials: H
authors.lastname: Baudisch
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Birkhahn
authors.forename: Rüdiger
authors.initials: R
authors.lastname: von Versen
authors.forename: Arne
authors.initials: A
authors.lastname: Hansen
authors.forename: Axel
authors.initials: A
authors.lastname: Pruss
mesh_terms: D016025:Bone Transplantation; D001842:Bone and Bones / Q000737:chemistry*; D002725:Chloroform / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000302:isolation & purification; D000432:Methanol
chemical_list: D008055:Lipids; D002725:Chloroform; D000432:Methanol