title: Genome relationships in polyploid Poa pratensis and other Poa species inferred from phylogenetic analysis of nuclear and chloroplast DNA sequences.
abstract: The genus Poa comprises approximately 500 species that occur throughout the world, including the widely grown Kentucky bluegrass (P. pratensis L.). Hybridization and polyploidization have played a prominent role in the evolution of this complex genus, but limited information is available regarding genome relationships in Poa. Thus, we amplified, cloned, and compared the DNA sequences of 2 nuclear genes (CDO504 and thioredoxin-like protein) and 2 chloroplast genome loci (ndhF and trnT-trnF) from 22 Poa species. Four distinct classes of sequences corresponding to 4 putative homoeologous loci from each nuclear gene were found within polyploid P. pratensis. Nuclear sequences from 15 other Poa species were found to group with at least 1 P. pratensis homoeolog, whereas 6 species displayed sequences not present in P. pratensis. The nuclear genome phylogenies presented here show the first evidence of diverse and related genomes in the genus Poa.
authors.affiliation: Department of Plants, Soils, and Biometeorology, Utah State University, Logan, UT 84322-4820, USA.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Patterson
authors.forename: Steven R
authors.initials: SR
authors.lastname: Larson
authors.forename: Paul G
authors.initials: PG
authors.lastname: Johnson
mesh_terms: D002467:Cell Nucleus / Q000235:genetics; D018742:DNA, Chloroplast / Q000235:genetics*; D018745:Genome, Plant; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D031785:Poa / Q000145:classification / Q000235:genetics*; D011123:Polyploidy
chemical_list: D018742:DNA, Chloroplast; D010940:Plant Proteins; C489904:NdhF protein, plant; D009245:NADH Dehydrogenase