title: Efficient micro-recovery and guanidination of peptides directly from MALDI target spots.
abstract: A method is presented for the recovery and subsequent guanidination of tryptic peptides from samples previously spotted on a matrix-assisted laser desorption/ionization (MALDI) target. The procedure is shown to have applicability to both in-solution and in-gel digests, yielding improved confidence in protein identification and sequence coverage in all instances. Recovery from the plate is essentially quantitative, with no residual analyte observed on the target spot. The technique is rapid, simple, and has extended applicability to other processing steps, including (but not limited to) derivatization for specific peptide studies or enzymatic treatment for subsequent profiling of posttranslational modifications. This method circumvents the failure of an initial analysis to generate suitable information and is particularly relevant for the analysis of precious samples.
authors.affiliation: Sigma-Aldrich Corporation, St. Louis, MO, USA.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Cockrill
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Foster
authors.forename: Justin
authors.initials: J
authors.lastname: Wildsmith
authors.forename: April R
authors.initials: AR
authors.lastname: Goodrich
authors.forename: John G
authors.initials: JG
authors.lastname: Dapron
authors.forename: Tom C
authors.initials: TC
authors.lastname: Hassell
authors.forename: William K
authors.initials: WK
authors.lastname: Kappel
authors.forename: Graham B I
authors.initials: GB
authors.lastname: Scott
mesh_terms: D019791:Guanidine / Q000737:chemistry*; D008832:Microchemistry / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*; D020539:Sequence Analysis, Protein / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010455:Peptides; D019791:Guanidine