[The influence of dermal template application on the p53 gene expression and apoptosis during wound repairing in burn patients].
OBJECTIVE
To observe dynamically the influence of the application of dermal template on the p53 gene expression and apoptosis during wound repairing in burn patients.


METHODS
Twenty burn patients were enrolled in the study and were divided into experiment (E, n = 11) and control (C, n = 9) groups. The escharectomy wounds in patients with 3rd degree burn in E group were covered with dermal template overlain with thin split-thickness autograft, while those in C group were covered with thin split-thickness autograft only. Specimens were harvested from wounds of both groups at 1st, 2nd, 3rd, 4th and 5th post operative week (POW). The P53 expression and the apoptosis were assessed respectively by immunohistochemistry and by TUNEL kit. The change in cell number was observed after HE staining.


RESULTS
The P53 expression increased gradually along with the wound healing process from 1st to 4th POW, which was significantly higher than that in C group at 2nd, 3rd, and 4th POW (P < 0.05), and it reached the peak at 4th POW. Fibroblasts underwent apoptosis at 1st POW in E group, while apoptosis of the endothelial cells occurred mainly at 2nd and 3rd POW. There was obvious difference in the rate of apoptosis between the two groups in 3rd and 4th POW (P < 0.05). The numbers of fibroblasts and vascular endothelial cells in E group were smaller than those in C group.


CONCLUSIONS
Application of dermal template overlain with thin split-thickness autograft to wounds could induce P53 expression and cell apoptosis, thereby reduce scar formation, resulting in improvement of the quality of wound healing.
Wang
Xi-qiao
XQ
Department of Burns, Rui Jin Hospital, The Second Shanghai Medical University, Shanghai 200025, P.R. China.
Su
Hai-tao
HT
Xiang
Jun
J
Wang
Run-xiu
RX
Qing
Chun
C
Lu
Shu-liang
SL
D000328:Adult; D017209:Apoptosis; D002056:Burns / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D020405:Dermis / Q000637:transplantation*; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014945:Wound Healing; D055815:Young Adult
D016159:Tumor Suppressor Protein p53