Expression of fractalkine (CX3CL1) and its receptor, CX3CR1, in periodontal diseased tissue.
The regulatory role of chemokines and chemokine receptors on specific leucocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that leucocytes infiltrating inflamed gingival tissue expressed marked levels of CX3CR1. In periodontal diseased tissue, the expression of fractalkine and CX3CR1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) and further, fractalkine was distributed mainly on endothelial cells, as shown by immunohistochemistry. Moreover, we can detect CX3CR1-expressing cells infiltrated in periodontal diseased tissue by immunohistochemical staining. Furthermore, fractalkine production by human umbilical vein endothelial cells (HUVEC) was up-regulated by pathogen-associated molecular patterns (PAMPs), including Porphyromonas gingivalis lipopolysaccharide (LPS). Thus, these findings suggested that CX3CR1 and the corresponding chemokine, fractalkine may have an important regulatory role on specific leucocyte migration into inflamed periodontal tissue.
Hosokawa
Y
Y
Department of Conservative Dentistry, Tokushima University School of Dentistry, Tokushima, Japan. hosokawa@dent.tokushima-u.ac.jp
Nakanishi
T
T
Yamaguchi
D
D
Nakae
H
H
Matsuo
T
T
D000942:Antigens, Bacterial / Q000494:pharmacology; D000073883:CX3C Chemokine Receptor 1; D018929:Cell Culture Techniques; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000032:analysis / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D018450:Disease Progression; D042783:Endothelial Cells / Q000276:immunology; D005881:Gingiva / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D010518:Periodontitis / Q000276:immunology*; D016966:Porphyromonas gingivalis / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis*; D019707:Receptors, Chemokine / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000942:Antigens, Bacterial; D000073883:CX3C Chemokine Receptor 1; C515538:CX3CL1 protein, human; C095235:CX3CR1 protein, human; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D012333:RNA, Messenger; D019707:Receptors, Chemokine
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