Yeast (ADPribosyl)ation: revisiting a controversial question.
The controversy about the occurrence of an (ADPribosyl)ating activity in yeast is still standing up. Here we discuss this topic on the basis of results obtained with classic experiments proposed over years as basis to characterize an (ADPribosyl)ation system in any organism. Independent results obtained in two different laboratories were in line with each other and went towards the occurrence of an active (ADPribosyl)ating system in Saccharomyces cerevisiae. In fact data collected from nuclear preparations of cultured cells matched those from baker's yeast and lyophilized yeast cells. Yeast (ADPribosyl)ating enzyme is a protein of 80-90 kDa, as determined by electrophoresis on polyacrylamide gel in sodium dodecyl sulphate, followed by immunoblotting with antibodies against anti-poly(ADPribose) polymerase catalytic site. It synthesizes products, that, after digestion with phosphodiesterase, co-migrates mainly with phosphoribosyl adenosine monophosphate after thin layer chromatography on silica gel plate.
Faraone-Mennella
Maria Rosaria
MR
Dipartimento di Chimica Biologica, Facolta' di Scienze M.F.N., Università FEDERICO II-80134 Napoli, Italy. faraone@unina.it
De Maio
Anna
A
Petrella
Anna
A
Syntichaki
Evangelia
E
Kerbalaeva
Albina M
AM
Nasmetova
S M
SM
Goulyamova
Toshkon G
TG
Farina
Benedetta
B
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D001345:Autoradiography; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D000246:Adenosine Diphosphate Ribose