title: Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography.
abstract: A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai was successfully established by using chloroform-methanol-water (4:3.5:2, v/v) as the two-phase solvent system. The method yielded 11.4 mg of epimedokoreanoside I, 46.5 mg of icariin and 17.7 mg of icariside II from 200 mg of the crude sample in one-step separation with the purity of 98.2%, 99.7% and 98.5%, respectively, as determined by high-performance liquid chromatography (HPLC). The structures of the flavonoids were identified by 1H NMR and 13C NMR.
authors.affiliation: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Renmin
authors.initials: R
authors.lastname: Liu
authors.forename: Aifeng
authors.initials: A
authors.lastname: Li
authors.forename: Ailing
authors.initials: A
authors.lastname: Sun
authors.forename: Jichun
authors.initials: J
authors.lastname: Cui
authors.forename: Lingyi
authors.initials: L
authors.lastname: Kong
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids