Synthesis and fluorescence studies of thiazole orange tethered onto oligonucleotide: development of a self-contained DNA biosensor on a fiber optic surface.
Thiazole orange dyes were derivatized with ethylene glycol linkers of various lengths, and were covalently linked to the 5' end of the oligonucleotides after solid-phase synthesis. The labeled oligonucleotides exhibited enhanced fluorescence upon hybridization to complementary DNA sequences at the surfaces of optical fibers, providing for a self-contained labeling strategy. It was determined that the melt temperatures of DNA hybrids using one mixed polypyrimidine base oligonucleotide sequence were dependent on the length of the tethers, and that the melt temperature could be shifted by more than 10 degrees C when tethers were introduced.
Wang
Xiaofeng
X
Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto, 3359 Mississauga Road North, Mississauga, ON, Canada L5L 1C6.
Krull
Ulrich J
UJ
D001483:Base Sequence; D052160:Benzothiazoles; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D005336:Fiber Optic Technology; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D055100:Optical Fibers; D011804:Quinolines; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000737:chemistry*
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011804:Quinolines; D013844:Thiazoles; C051000:thiazole orange; D004247:DNA