[The establishment and clinical application of multicolor fluorescence in situ hybridization].
OBJECTIVE
To establish a rapid and convenient method of multi-color fluorescence in situ hybridization (FISH) on several different tissue samples: peripheral blood samples, amniotic fluid, embryos and bone marrow samples.


METHODS
FISH analysis was carried out on different tissue samples, using probes specific for chromosomes 13,18,21,X and Y or for BCR/ABL gene.


RESULTS
FISH analysis could reveal hybridization signals on metaphase chromosomes and interphase nuclei, it is also possible to detect chromosome aberrations.


CONCLUSIONS
Multi-color FISH is clinically useful tool, which can be used as an adjunct to conventional chromosome analysis for prenatal diagnosis , preimplantation genetic diagnosis and diagnosis of leukemia.
Li
Qing
Q
Guangzhou Second People's Hospital, Guangzhou Research Institute of Obstetrics and Gynecology, Guangzhou 510150, China. qinglicn@sina.com.cn
Sun
Xiao-fang
XF
Chen
Xin-jie
XJ
Kong
Shu
S
Zheng
Yu-hong
YH
Huang
Yan-yi
YY
D000328:Adult; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*