Synthesis of arabinitol 1-phosphate and its use for characterization of arabinitol-phosphate dehydrogenase.
D-arabinitol 1-phosphate (Ara-ol1-P), a substrate for D-arabinitol-phosphate dehydrogenase (APDH), was chemically synthesized from D-arabinonic acid in five steps (O-acetylation, chlorination, reduction, phosphorylation, and de-O-acetylation). Ara-ol1-P was used as a substrate for the characterization of APDH from Bacillus halodurans. APDH converts Ara-ol1-P to xylulose 5-phosphate in the oxidative reaction; both NAD(+) and NADP(+) were accepted as co-factors. Kinetic parameters for the oxidative and reductive reactions are consistent with a ternary complex mechanism.
Soroka
Nikolai V
NV
Molecular and Radiation Biology Division, Petersburg Nuclear Physics Institute, Russian Academy of Science, Gatchina 188300, Russia.
Kulminskaya
Anna A
AA
Eneyskaya
Elena V
EV
Shabalin
Konstantin A
KA
Uffimtcev
Andrei V
AV
Povelainen
Mira
M
Miasnikov
Andrei N
AN
Neustroev
Kirill N
KN
D001407:Bacillus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016983:Enterococcus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010428:Pentosephosphates / Q000138:chemical synthesis* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013237:Stereoisomerism; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014996:Xylulose / Q000378:metabolism
D001426:Bacterial Proteins; D010428:Pentosephosphates; D011994:Recombinant Proteins; D009243:NAD; D009249:NADP; D014996:Xylulose; D013401:Sugar Alcohol Dehydrogenases