title: WRKY group IId transcription factors interact with calmodulin.
abstract: Calmodulin (CaM) is a ubiquitous Ca(2+)-binding protein known to regulate diverse cellular functions by modulating the activity of various target proteins. We isolated a cDNA encoding AtWRKY7, a novel CaM-binding transcription factor, from an Arabidopsis expression library with horseradish peroxidase-conjugated CaM. CaM binds specifically to the Ca(2+)-dependent CaM-binding domain (CaMBD) of AtWRKY7, as shown by site-directed mutagenesis, a gel mobility shift assay, a split-ubiquitin assay, and a competition assay using a Ca2+/CaM-dependent enzyme. Furthermore, we show that the CaMBD of AtWRKY7 is a conserved structural motif (C-motif) found in group IId of the WRKY protein family.
authors.affiliation: Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsarg National University, Jinju 660-701, Republic of Keorea.
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Park
authors.forename: Ju Huck
authors.initials: JH
authors.lastname: Lee
authors.forename: Jae Hyuk
authors.initials: JH
authors.lastname: Yoo
authors.forename: Byeong Cheol
authors.initials: BC
authors.lastname: Moon
authors.forename: Man Soo
authors.initials: MS
authors.lastname: Choi
authors.forename: Yun Hwan
authors.initials: YH
authors.lastname: Kang
authors.forename: Sang Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Ho Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
authors.forename: Chae Oh
authors.initials: CO
authors.lastname: Lim
authors.forename: Moo Je
authors.initials: MJ
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D010446:Peptide Fragments; D014157:Transcription Factors; D025801:Ubiquitin; C498414:WRKY7 protein, Arabidopsis