title: Sec6 mutations and the Drosophila exocyst complex.
abstract: To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.
authors.affiliation: Division of Neuroscience, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Mala
authors.initials: M
authors.lastname: Murthy
authors.forename: Ravi
authors.initials: R
authors.lastname: Ranjan
authors.forename: Natalie
authors.initials: N
authors.lastname: Denef
authors.forename: Misao E L
authors.initials: ME
authors.lastname: Higashi
authors.forename: Trudi
authors.initials: T
authors.lastname: Schupbach
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Schwarz
mesh_terms: D000483:Alleles; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D003433:Crosses, Genetic; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D000939:Epitopes / Q000737:chemistry; D017353:Gene Deletion; D017930:Genes, Reporter; D005838:Genotype; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D008957:Models, Genetic; D015233:Models, Statistical; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D016366:Open Reading Frames; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D019076:Transgenes; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; D000939:Epitopes; D008565:Membrane Proteins; C502950:Sec5 protein, Drosophila; C502606:Sec6 protein, Drosophila; C503568:Sec8 protein, Drosophila; D033921:Vesicular Transport Proteins