title: Use of a microbubble agent to increase the effects of high intensity focused ultrasound on liver tissue.
abstract: In order to find out whether high intensity focused ultrasound (HIFU) might be useful against hepatocellular carcinoma, we analyzed the effect of a microbubble agent (Levovist) on the temperature rise and tissue necrosis induced by HIFU. Rabbits were given 7 ml Levovist (300 mg/ml) or saline intravenously. Up to six areas per rabbit liver were exposed to HIFU for 60 s (2.18 MHz, I(SPTA)=400 W/cm(2)). The volume of the tissue coagulated by HIFU was measured 10 min after the start of HIFU. HIFU-induced lesions were larger in the animals given Levovist: (mm(3), Levovist versus saline) 371+/-104 versus 166+/-71 (P<0.001). Temperatures in the animals given Levovist were also higher 60 s after the start of exposure: ( degrees C, Levovist versus saline) 20.3+/-3.5 versus 13.2+/-3.8 (P<0.001). The amount of damage differed greatly, but the pathological changes caused by HIFU with Levovist were the same as those caused by HIFU with saline. Hemorrhagic areas and implosion cysts were seen, and many cells had been disrupted or destroyed. Microbubble agents developed for diagnostic uses could also be used in anticancer therapy.
authors.affiliation: Department of Mechanical Engineering, The University of Tokyo, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Maruyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Takegami
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitsui
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hanajiri
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Nagawa
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001831:Body Temperature / Q000187:drug effects; D003287:Contrast Media; D006979:Hyperthermia, Induced; D008099:Liver / Q000000981:diagnostic imaging* / Q000473:pathology; D045423:Microbubbles; D009336:Necrosis; D011134:Polysaccharides; D011817:Rabbits; D012965:Sodium Chloride; D013997:Time Factors; D014464:Ultrasonic Therapy; D014463:Ultrasonography
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D011134:Polysaccharides; C059891:SHU 508; D012965:Sodium Chloride
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