title: Pathogenesis of swine influenza virus subtype H1N2 infection in pigs.
abstract: The purpose of this study was to elucidate pathogenesis and viral distribution in pigs infected with swine influenza virus subtype H1N2, over a period of 10 days, by morphometric analysis and in-situ hybridization. Fifteen colostrum-deprived pigs aged 3 weeks were inoculated intranasally with virus. Pneumonia was severe at 1 day post-inoculation (dpi), moderate at 3 and 5 dpi, and mild at 7 and 10 dpi. The pulmonary lesion score was correlated with the score of cells positive by in-situ hybridization for swine influenza virus (r(s)= 0.9114, P< 0.05). The distribution of swine influenza virus varied according to the duration of infection. At 1 and 3 dpi, hybridization signals were detected mainly in the bronchial and bronchiolar epithelial cells, but they were detected mainly in the pneumocytes and macrophages (alveolar and interstitial) at 7 and 10 dpi. The results confirmed that swine influenza virus subtype H1N2, isolated in Korea, is a virulent pathogen causing severe pneumonia.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu 151-742, Seoul, Republic of Korea.
authors.forename: K
authors.initials: K
authors.lastname: Jung
authors.forename: Y
authors.initials: Y
authors.lastname: Ha
authors.forename: C
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001980:Bronchi / Q000473:pathology / Q000821:virology; D002452:Cell Count / Q000662:veterinary; D004848:Epithelium / Q000473:pathology / Q000821:virology; D017403:In Situ Hybridization / Q000662:veterinary; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000473:pathology* / Q000821:virology
chemical_list: D012367:RNA, Viral