Salmonella enterica Serovar Typhimurium infection of dendritic cells leads to functionally increased expression of the macrophage-derived chemokine.
Gene expression in murine dendritic cells (DCs) infected with green fluorescent protein-expressing Salmonella enterica serovar Typhimurium BRD509 was studied by mRNA differential display. Infected DCs were sorted from uninfected cells by flow cytometry. The mRNA expression patterns of infected and uninfected cells revealed a number of differentially expressed transcripts, which included the macrophage-derived chemokine (MDC). Up-regulation of MDC transcription in infected DCs was confirmed by Northern blotting, and the kinetics of MDC expression was examined by real-time reverse transcription-PCR, with which 31- and 150-fold increases were detected at 2 and 6 h postinfection, respectively. The increased release by DCs of MDC into culture media was detected by an enzyme-linked immunosorbent assay. The biological activity of MDC was investigated in in vitro and in vivo assays. In vitro, supernatants from S. enterica serovar Typhimurium-infected DCs were chemoattractive to T cells, and neutralization of MDC in these supernatants inhibited T-cell migration. Passive transfer of anti-MDC antibody to mice infected with BRD509 revealed that neither growth of the bacterium nor resistance of the mice to reinfection was affected and that in vivo inhibition of MDC did not affect T-cell responses, as measured by the gamma interferon ELISPOT method 3 days after challenge infection.
Fu
Guo
G
CRC for Vaccine Technology, Parkville, Victoria 3010, Australia.
Wijburg
Odilia L C
OL
Cameron
Paul U
PU
Price
Jason D
JD
Strugnell
Richard A
RA
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000097:blood / Q000276:immunology; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000472:pathogenicity*; D013601:T-Lymphocytes / Q000276:immunology; D015854:Up-Regulation
D000906:Antibodies; C515483:Ccl22 protein, mouse; D054422:Chemokine CCL22; D019742:Chemokines, CC; D049452:Green Fluorescent Proteins
J Invest Dermatol. 1999 Dec;113(6):991-8
10594742
Science. 1999 Dec 10;286(5447):2098-102
10617422
Mol Microbiol. 2000 Jan;35(1):69-78
10632878
Adv Exp Med Biol. 1999;473:261-74
10659367
J Exp Med. 2000 Feb 21;191(4):613-24
10684853
Immunity. 2000 Feb;12(2):121-7
10714678
FEMS Immunol Med Microbiol. 2000 Apr;27(4):313-20
10727887
Eur J Immunol. 2000 Mar;30(3):944-53
10741413
Eur J Immunol. 2000 Apr;30(4):1030-9
10760790
J Immunol. 2000 May 15;164(10):5362-8
10799899
Infect Immun. 2000 Jun;68(6):3744-7
10816540
J Immunol. 2000 Jun 1;164(11):5894-904
10820271
Annu Rev Immunol. 2000;18:767-811
10837075
Med Microbiol Immunol. 2000 Jun;188(4):191-6
10917156
Mol Microbiol. 2000 Oct;38(1):31-40
11029688
Infect Immun. 2000 Nov;68(11):6311-20
11035740
Nat Immunol. 2001 Feb;2(2):95-101
11175800
Nat Immunol. 2001 Apr;2(4):361-7
11276208
Cell. 2001 Aug 10;106(3):255-8
11509172
J Clin Invest. 2001 Sep;108(6):917-27
11560961
Microbes Infect. 2001 Nov-Dec;3(14-15):1239-48
11755412
Microbes Infect. 2001 Nov-Dec;3(14-15):1321-6
11755421
Mol Microbiol. 2002 Apr;44(1):283-96
11967086
J Immunol. 2002 Jul 1;169(1):108-16
12077235
J Immunol. 2002 Sep 15;169(6):3275-83
12218147
Cell Microbiol. 2003 Dec;5(12):933-45
14641178
Eur J Immunol. 1973 Oct;3(10):645-9
4587740
J Exp Med. 1974 May 1;139(5):1189-203
4596512
Infect Immun. 1990 May;58(5):1323-6
2182542
Science. 1992 Aug 14;257(5072):967-71
1354393
Nucleic Acids Res. 1993 Jul 11;21(14):3269-75
8341601
Gastroenterology. 1994 Oct;107(4):915-23
7926481
Nucleic Acids Res. 1995 Aug 25;23(16):3357-8
7545288
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9833-8
8790417
Mol Microbiol. 1996 Sep;21(5):1101-15
8885278
J Immunol. 1997 May 1;158(9):4229-36
9126984
J Exp Med. 1997 May 5;185(9):1595-604
9151897
J Exp Med. 1997 Aug 18;186(4):569-80
9254655
J Biol Chem. 1997 Oct 3;272(40):25229-37
9312138
J Exp Med. 1998 Jan 5;187(1):129-34
9419219
J Biol Chem. 1998 Jan 16;273(3):1764-8
9430724
J Exp Med. 1998 Mar 16;187(6):875-83
9500790
Nature. 1998 Mar 19;392(6673):245-52
9521319
J Leukoc Biol. 1998 Jul;64(1):49-54
9665274
J Immunol Methods. 1999 Feb 1;223(1):77-92
10037236
Science. 1999 Apr 30;284(5415):819-22
10221917
Eur J Immunol. 1999 Apr;29(4):1107-15
10229077
Immunol Today. 1999 Jun;20(6):254-7
10354549
Microbes Infect. 2001 Nov-Dec;3(14-15):1353-60
11755425
Infect Immun. 1998 Aug;66(8):3758-66
9673259
J Exp Med. 1998 Aug 3;188(3):451-63
9687523
Blood. 1998 Oct 15;92(8):2668-71
9763548
J Immunol. 1998 Nov 1;161(9):5027-38
9794440
Eur J Immunol. 1999 Jan;29(1):180-8
9933099