title: Stability and effectiveness against bacterial adhesion of poly(ethylene oxide) coatings in biological fluids.
abstract: Poly(ethylene oxide) (PEO) coatings have been shown to reduce the adhesion of different microbial strains and species and thus are promising as coatings to prevent biomaterial-centered infection of medical implants. Clinically, however, PEO coatings are not yet applied, as little is known about their stability and effectiveness in biological fluids. In this study, PEO coatings coupled to a glass substratum through silyl ether bonds were exposed for different time intervals to saliva, urine, or phosphate-buffered saline (PBS) as a reference at 37 degrees C. After exposure, the effectiveness of the coatings against bacterial adhesion was assessed in a parallel plate flow chamber. The coatings appeared effective against Staphylococcus epidermidis adhesion for 24, 48, and 0.5 h in PBS, urine, and saliva, respectively. Using XPS and contact-angle measurements, the variations in effectiveness could be attributed to conditioning film formation. The overall short stability results from hydrolysis of the coupling of the PEO chains to the substratum.
authors.affiliation: Department of Biomedical Engineering, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Astrid
authors.initials: A
authors.lastname: Roosjen
authors.forename: Joop
authors.initials: J
authors.lastname: de Vries
authors.forename: Henny C
authors.initials: HC
authors.lastname: van der Mei
authors.forename: Willem
authors.initials: W
authors.lastname: Norde
authors.forename: Henk J
authors.initials: HJ
authors.lastname: Busscher
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001672:Biocompatible Materials; D001826:Body Fluids / Q000502:physiology*; D004355:Drug Stability; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000737:chemistry*; D012463:Saliva / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D013499:Surface Properties; D013501:Surface-Active Agents
chemical_list: D001672:Biocompatible Materials; D013501:Surface-Active Agents; D011092:Polyethylene Glycols