title: [Analysis of heading time genotype for a rice photoperiod and thermo--sensitive male sterile line PeiAi64S].
abstract: PeiAi64S, a photoperiod and thermo-sensitive genic male sterile, has been wildly applied to hybrid rice seed production in China, but its photoperiod-sensitivity gene for heading date in this sterile line was still unknown. This definitely limited the further use of this sterile line in breeding practice and re-production of hybrid seeds. To solve this problem, using heading time nearly isogenic lines EGO - EG7, ER - LR, T65 - T65m and six heading date QTL-isogenic lines, NIL (Hd1) - NIL (Hd6) with the genes of Nipponbare but Hd1 - Hd6 genes from Kasalath respectively, we performed a genetic analysis of PeiAi64S with special reference to photoperiod-sensitivity loci, in natural long days at Nanjing (32 degrees N) where the average day-length is about 14 h and in natural short days at Linshui county, Hainan province(18 degrees 29'N), where the average day-length is about 11.6 h during the course of rice growing respectively. The F1 and F2 generations from the crosses 'PeiAi64S x heading time nearly isogenic lines' were subjected to genetic analyses. Experimental results showed that PeiAi64S carries photoperiod-sensitivity allele gene E1 and E3 and dominant earliness gene Ef-1 in E1 and E3 and Ef-1 loci, respectively, and a photoperiod insensitivity allele Se-1 degrees in Se-1 locus. Meanwhile, the photoperiod-sensitivity gene E1 and photoperiod-insensitivity gene Se-1e in PeiAi64S were also identified by crossing with the NIL(Hd1) and NIL(Hd4). In addition, a recessive inhibitor for photoperiod-sensitive gene E1 or Se-1(n) and other modified photoperiod-sensitive genes: i-Se-1, E3, Hd3 (En-Se-1), Hd5 and Hd6, were identified in PeiAi64S by crossing with QTL nearly isogenic lines: NIL(Hd2), NIL (Hd3), NIL (Hd5) and NIL( Hd6), The results indicated that the genotype of PeiAi64S's heading date was: E1E1e2e2E3E3Se-1(e)Se-1(e)Ef-1 Ef-1 i-Se-1 i-Se-1.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Xu
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.forename: Guo-Gang
authors.initials: GG
authors.lastname: Wang
authors.forename: Shi-Jia
authors.initials: SJ
authors.lastname: Liu
authors.forename: Liang-Ming
authors.initials: LM
authors.lastname: Chen
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Wang
authors.forename: Lin-Guang
authors.initials: LG
authors.lastname: Luo
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Wan
mesh_terms: D001947:Breeding; D003433:Crosses, Genetic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D006824:Hybridization, Genetic; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D017440:Photoperiod; D051479:Plant Infertility; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D013696:Temperature