Identification and characterization of undifferentiated mast cells in mouse bone marrow.
Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34(+), CD13(+), and c-kit(+), and does not express FcepsilonRI. However, by polymerase chain reaction (PCR) the cell contains message for the alpha and beta subunits of FcepsilonRI, mast cell-specific proteases, and carboxypeptidase A. Morphologically, this cell has a large nucleus, little cytoplasm, few cytoplasmic organelles, and no cytoplasmic granules. In vitro, in the presence of interleukin-3 (IL-3) and stem cell factor (SCF) these cells differentiate only into a granulated mast cell that now expresses CD13, c-kit, mast cell-specific gangliosides, FcepsilonRI, and binds immunoglobulin E (IgE). When injected into lethally irradiated mice, these cells are able to reconstitute the mast cell population in the spleen.
Jamur
Maria Célia
MC
Faculdade de Medicina de Ribeirão Preto, Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Grodzki
Ana Cristina G
AC
Berenstein
Elsa H
EH
Hamawy
Majed M
MM
Siraganian
Reuben P
RP
Oliver
Constance
C
D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D018826:CD13 Antigens; D002454:Cell Differentiation; D005260:Female; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D008297:Male; D008407:Mast Cells / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D015388:Organelles / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D017455:Receptors, IgE / Q000235:genetics
D018952:Antigens, CD34; D012333:RNA, Messenger; D017455:Receptors, IgE; D018826:CD13 Antigens