Simultaneous determination of five marker constituents in traditional Chinese medicinal preparation Le-Mai granule by high performance liquid chromatography.
An HPLC method for the simultaneous determination of five marker constituents was established for the quality control of traditional Chinese medicinal preparation Le-Mai granule. The marker constituents were danshensu, protocatechuic acid and protocatechualdehyde from Salviae miltiorrhizae bunge; paeoniflorin from Radix paeoniae rubra and ferulic acid from Rhizoma chuanxiong. Extracted samples were successfully separated on a Diamonsil C18 column (150 mm x 4.6 mm i.d., 5 microm) at 25 degrees C. The mobile phase was a mixture of methanol and 1.0% acetic acid employing gradient elution at a flow rate of 1.0 mL/min. Detection was accomplished with a diode-array detector and chromatograms were recorded at 230, 262, 280 and 322 nm. The compounds were identified by comparing their retention times and UV spectra in the 200-400 nm range with authentic standards. Regression equations revealed good linear relationship (correlation coefficients: 0.9993-0.9999) between the peak areas of the constituents and their concentrations. The average recoveries (n=3) were between 96.2 and 102.5%. The proposed method has been successfully applied to the simultaneous determination of the five marker constituents in three lots of Le-Mai granule.
Shi
Zhihong
Z
Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
He
Jiantao
J
Zhao
Meiping
M
Chang
Wenbao
W
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D008516:Medicine, Chinese Traditional
D004365:Drugs, Chinese Herbal