Cross-serotype neutralization of dengue virus in Aotus nancymae monkeys.
Previously, we observed that serum from humans immune to dengue serotype 1 (dengue-1) neutralized the American genotype of dengue serotype 2 (American-2) to a greater extent than it neutralized the Asian genotype of dengue serotype 2 (Asian-2). To determine if this activity is protective, Aotus nancymae monkeys were infected with dengue-1 followed by either American-2 or Asian-2. Dengue-1-infected animals produced antibody with neutralizing titers of 2656 antibodies against dengue-1, 409 against American-2, and <20 against Asian-2. Infection with American-2 did not produce detectable viremia in either dengue-1-immune or dengue-1-naive animals. These findings support the hypothesis that dengue-1 immunity might have prevented disease or altered the severity of disease in individuals sequentially infected with dengue-1 and American-2.
Kochel
Tadeusz J
TJ
US Naval Medical Research Center Detachment, Lima, Peru. kochelt@nmrc.navy.mil
Watts
Douglas M
DM
Gozalo
Alfonso S
AS
Ewing
Daniel F
DF
Porter
Kevin R
KR
Russell
Kevin L
KL
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D016645:Aotidae; D003429:Cross Reactions; D003715:Dengue / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D003716:Dengue Virus / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity; D005260:Female; D006801:Humans; D008297:Male; D009500:Neutralization Tests; D012703:Serotyping; D014766:Viremia / Q000276:immunology / Q000517:prevention & control / Q000821:virology
D000914:Antibodies, Viral