title: Expression of fractalkine (CX3CL1) and its receptor, CX3CR1, in periodontal diseased tissue.
abstract: The regulatory role of chemokines and chemokine receptors on specific leucocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that leucocytes infiltrating inflamed gingival tissue expressed marked levels of CX3CR1. In periodontal diseased tissue, the expression of fractalkine and CX3CR1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) and further, fractalkine was distributed mainly on endothelial cells, as shown by immunohistochemistry. Moreover, we can detect CX3CR1-expressing cells infiltrated in periodontal diseased tissue by immunohistochemical staining. Furthermore, fractalkine production by human umbilical vein endothelial cells (HUVEC) was up-regulated by pathogen-associated molecular patterns (PAMPs), including Porphyromonas gingivalis lipopolysaccharide (LPS). Thus, these findings suggested that CX3CR1 and the corresponding chemokine, fractalkine may have an important regulatory role on specific leucocyte migration into inflamed periodontal tissue.
authors.affiliation: Department of Conservative Dentistry, Tokushima University School of Dentistry, Tokushima, Japan. hosokawa@dent.tokushima-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
authors.forename: D
authors.initials: D
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Nakae
authors.forename: T
authors.initials: T
authors.lastname: Matsuo
mesh_terms: D000942:Antigens, Bacterial / Q000494:pharmacology; D000073883:CX3C Chemokine Receptor 1; D018929:Cell Culture Techniques; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000032:analysis / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D018450:Disease Progression; D042783:Endothelial Cells / Q000276:immunology; D005881:Gingiva / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D010518:Periodontitis / Q000276:immunology*; D016966:Porphyromonas gingivalis / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis*; D019707:Receptors, Chemokine / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000942:Antigens, Bacterial; D000073883:CX3C Chemokine Receptor 1; C515538:CX3CL1 protein, human; C095235:CX3CR1 protein, human; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D012333:RNA, Messenger; D019707:Receptors, Chemokine
references.citation: Eur J Immunol. 2000 Jan;30(1):87-97
references.pmid: 10602030
references.citation: J Exp Med. 1998 Oct 19;188(8):1413-9
references.pmid: 9782118
references.citation: J Immunol. 2000 Apr 15;164(8):4313-20
references.pmid: 10754331
references.citation: Annu Rev Immunol. 2000;18:593-620
references.pmid: 10837070
references.citation: J Dent Res. 2000 Aug;79(8):1548-55
references.pmid: 11023273
references.citation: Blood. 2001 Jan 15;97(2):367-75
references.pmid: 11154210
references.citation: J Clin Invest. 2001 May;107(9):1173-81
references.pmid: 11342581
references.citation: Crit Rev Oral Biol Med. 2001;12(2):125-35
references.pmid: 11345523
references.citation: J Immunol. 1999 Jan 1;162(1):186-94
references.pmid: 9886385
references.citation: Adv Immunol. 2001;78:57-110
references.pmid: 11432208
references.citation: Clin Exp Immunol. 2001 Jul;125(1):134-41
references.pmid: 11472436
references.citation: Clin Exp Immunol. 2002 Jun;128(3):548-54
references.pmid: 12067311
references.citation: Cytokine. 2002 Oct 21;20(2):70-7
references.pmid: 12445801
references.citation: J Periodontal Res. 2003 Apr;38(2):210-7
references.pmid: 12608917
references.citation: J Leukoc Biol. 2003 Jul;74(1):16-24
references.pmid: 12832438
references.citation: J Rheumatol. 2003 Sep;30(9):1918-27
references.pmid: 12966591
references.citation: J Clin Periodontol. 1985 Sep;12(8):648-59
references.pmid: 3863838
references.citation: J Periodontol. 1992 Aug;63(8):686-9
references.pmid: 1507049
references.citation: J Periodontal Res. 1993 Nov;28(6 Pt 2):478-86
references.pmid: 7505322
references.citation: Clin Exp Immunol. 1996 Mar;103(3):422-8
references.pmid: 8608641
references.citation: Nature. 1997 Feb 13;385(6617):640-4
references.pmid: 9024663
references.citation: Nature. 1997 Jun 5;387(6633):611-7
references.pmid: 9177350
references.citation: J Clin Periodontol. 1998 Feb;25(2):134-44
references.pmid: 9495612
references.citation: J Immunol. 2000 Apr 15;164(8):4055-62
references.pmid: 10754298