Characterization of the effect of deoxynivalenol on selected male reproductive endpoints.
The effect of deoxynivalenol (DON) exposure on male reproductive function was assessed in the rat. Male rats were divided into a control group (n=15 rats) and four treatment groups (0.5 mg/kg, n=15; 1.0 mg/kg, n=15; 2.5 mg/kg, n=15; and 5.0 mg/kg DON, n=16) and exposed to DON daily for 28 days via gastric intubation. Both body weight gain and the final body weight of animals in the 5.0 mg/kg dose group and feed consumption in animals in the 2.5 mg/kg and 5.0 mg/kg dose groups were significantly reduced compared to controls. Fluid consumption was not affected in any of the treated groups. Epididymal and seminal vesicle weights expressed per gram of body weight and brain weight were significantly reduced, compared to control weights, in animals from the 2.5 and 5.0 mg/kg dose groups while prostate weight expressed per gram of brain weight and body weight was significantly lower than controls only in the 5.0 mg/kg dose group. A statistically significant, dose-related decrease in homogenization resistant testicular spermatid counts, spermatid numbers, absolute cauda epididymal sperm numbers and cauda epididymal sperm numbers per gram of cauda epididymis was observed in the 5.0 mg/kg DON treatment group. Sperm tail abnormalities (broken tails) in the 5.0 mg/kg dose group were significantly higher than in the control group. Sperm swimming speed (VSL and VCL) was significantly increased only in the 2.5 mg/kg dose group. Serum FSH and LH concentrations were increased in a dose dependent manner across all treated groups while serum testosterone concentrations were decreased in a dose-related manner across all dose groups. An increase in germ cell degeneration, sperm retention and abnormal nuclear morphology was observed in the 2.5 mg/kg and 5.0 mg/kg dose groups. Treatment related effects included lesions in the non-glandular stomach, thymic lymphoid depletion and splenic hematopoiesis in the 5.0 mg/kg treatment group.
Sprando
Robert L
RL
Food and Drug Administration, Division of Toxicology and Nutritional Product Studies, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, Laurel, MD 20708, USA. rsprando@cfsan.fda.gov
Collins
Thomas F X
TF
Black
Thomas N
TN
Olejnik
Nicholas
N
Rorie
James I
JI
Eppley
Robert M
RM
Ruggles
Dennis I
DI
D000818:Animals; D001835:Body Weight; D023381:Endpoint Determination; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000002:abnormalities; D013737:Testis / Q000187:drug effects / Q000473:pathology*; D014255:Trichothecenes / Q000633:toxicity*
D014255:Trichothecenes; C007262:deoxynivalenol