A prototype prophylactic anti-influenza preparation in aerosol form on the basis of Abies sibirica polyprenols.
This research investigates a promising antiviral compound based on polyprenols from Siberian silver fir (Abies sibirica). The physico-chemical characteristics of a preparation developed in aerosol form and an estimation of its protective efficacy against aerosol challenge of laboratory animals are presented. It is shown that (1) by using a simple ultrasonic disperser one can obtain aerosol of three formulations studied with about 70% of its mass accumulated in the size range below 1.8 microm; (2) 40-100% of aerosol particles contain preparation for different formulations; (3) after delivering under specified schedules, the preparations as developed can protect up to 100% of mice against 5 LD(50) of influenza A/Aichi/2/68 (H3N2) virus aerosol infection. Animals inhaled twice the preparation doses (which were 100 times lower than injection ones of the same efficacy) and did not exceed 10 microg/mouse. It was shown that the mode of action of this immunomodulating preparation was nonspecific stimulation of immune cells' various activities.
Safatov
A S
AS
State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk, Russia. safatov@vector.nsc.ru
Boldyrev
A N
AN
Bulychev
L E
LE
Buryak
G A
GA
Kukina
T P
TP
Poryvaev
V D
VD
P'yankov
O V
OV
Raldugin
V A
VA
Ryzhikov
A B
AB
Sergeev
A N
AN
Shishkina
L N
LN
Tolstikov
G A
GA
Zhukov
V A
VA
D028202:Abies; D000336:Aerosols; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D005260:Female; D009980:Influenza A virus; D008297:Male; D051379:Mice; D009330:Nebulizers and Vaporizers; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D008517:Phytotherapy; D028321:Plant Preparations
D000336:Aerosols; D000998:Antiviral Agents; D028321:Plant Preparations