title: CD14-dependent modulation of transcriptional activities of endogenous retroviruses in the lung after injury.
abstract: Lipopolysaccharide (LPS) plays a central role in the pathogenesis of distant organs after burn. Recent studies demonstrated the regulation of mouse endogenous retroviruses (MuERVs) in several organs after burn. In this study, the role of CD14, a LPS receptor, in burn-mediated regulation of MuERV expression in the lung was investigated. CD14 knockout (KO) and wild type (WT) mice were subjected to burn followed by RT-PCR analysis of alterations in the MuERV expression in the lung 1 day after injury. Even without injury, CD14 KO mice had a unique profile of MuERV expression compared to WT. Three bands (Lung-1, Lung-2, and Lung-3) in CD14 KO were downregulated after injury. Lung-2 and Lung-3 transcripts were almost identical to 2 previously described defective env transcripts of MuERVs, respectively. The Lung-1-1 transcript was a double spliced message generated by the env and a set of novel splicing signals, whereas the Lung-1-2 transcript was a defective env transcript. Only the Lung-1-1 transcript had a significant ORF capable of encoding a gag-pol fusion polypeptide. Putative proviral sequences of Lung-1-1 and Lung-1-2 transcripts were mapped to chromosomes 4 and 11, respectively. The results from this study suggest that the absence of CD14 expression in CD14 KO mice contributes to the transcriptional regulation of MuERVs in the lung after injury.
authors.affiliation: Burn Research, Shriners Hospitals for Children Northern California, Department of Surgery, University of California at Davis, Sacramento, CA 95817, USA.
authors.forename: Kiho
authors.initials: K
authors.lastname: Cho
authors.forename: Tam N
authors.initials: TN
authors.lastname: Pham
authors.forename: David G
authors.initials: DG
authors.lastname: Greenhalgh
mesh_terms: D000818:Animals; D001483:Base Sequence; D002056:Burns / Q000821:virology*; D002874:Chromosome Mapping; D020077:Endogenous Retroviruses / Q000235:genetics* / Q000502:physiology; D005260:Female; D015685:Fusion Proteins, gag-pol / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D015752:Genes, env; D018950:Lipopolysaccharide Receptors / Q000502:physiology*; D008168:Lung / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D011533:Proviruses / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: D015685:Fusion Proteins, gag-pol; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D012367:RNA, Viral
references.citation: Burns. 2003 Feb;29(1):1-14
references.pmid: 12543039
references.citation: Am J Pathol. 1983 Sep;112(3):347-56
references.pmid: 6311020
references.citation: Clin Diagn Lab Immunol. 1998 Mar;5(2):181-5
references.pmid: 9521140
references.citation: Acta Neurol Scand. 1993 Sep;88(3):190-8
references.pmid: 8256555
references.citation: J Virol. 1999 May;73(5):4327-40
references.pmid: 10196331
references.citation: World J Gastroenterol. 2002 Jun;8(3):546-50
references.pmid: 12046089
references.citation: Nature. 1989 Apr 6;338(6215):505-8
references.pmid: 2538760
references.citation: Cell. 1997 Jul 25;90(2):303-13
references.pmid: 9244304
references.citation: Shock. 2000 Aug;14(2):182-6
references.pmid: 10947164
references.citation: J Burn Care Rehabil. 1994 Nov-Dec;15(6):493-8
references.pmid: 7852452
references.citation: Biol Reprod. 1994 Jul;51(1):152-7
references.pmid: 7522592
references.citation: J Surg Res. 2002 May 1;104(1):53-62
references.pmid: 11971678
references.citation: Curr Opin Genet Dev. 1999 Dec;9(6):657-63
references.pmid: 10607616
references.citation: Virology. 1995 Aug 20;211(2):589-92
references.pmid: 7645262
references.citation: Virus Res. 2003 Jun;93(2):189-98
references.pmid: 12782367
references.citation: J Virol. 1995 Nov;69(11):7138-46
references.pmid: 7474134
references.citation: Proc Natl Acad Sci U S A. 1989 May;86(10):3862-6
references.pmid: 2542949
references.citation: Arthritis Rheum. 1992 Dec;35(12):1483-91
references.pmid: 1472125
references.citation: Science. 2001 Feb 16;291(5507):1304-51
references.pmid: 11181995
references.citation: Ann Surg. 1997 May;225(5):530-41; discussion 541-3
references.pmid: 9193181
references.citation: FASEB J. 1992 May;6(8):2537-44
references.pmid: 1592206
references.citation: Science. 1973 Mar 30;179(4080):1332-3
references.pmid: 4631426
references.citation: Nature. 2001 Feb 15;409(6822):860-921
references.pmid: 11237011