title: Mast cell distribution in normal adult skin.
abstract: OBJECTIVE
  To investigate mast cell distribution in normal adult skin to provide a reference range for comparison with mastocytosis.
  
  
  METHODS
  Mast cells (MCs) were counted in uninvolved skin adjacent to basal cell carcinomas and other dermatological disorders in adults.
  
  
  RESULTS
  There was an uneven distribution of MCs in different body sites using the anti-tryptase monoclonal antibody technique. Numbers of MCs on the trunk, upper arm, and upper leg were similar, but were significantly different from those found on the lower leg and forearm. Two distinct groups were formed--proximal and distal. There were 77.0 MCs/mm2 at proximal body sites and 108.2 MCs/mm2 at distal sites. Adjusted for the adjacent diagnosis and age, this difference was consistent. The numbers of MCs in uninvolved skin adjacent to basal cell carcinomas and other dermatological disorders were not different from those in the control group. Differences in the numbers of MCs between the distal and the proximal body sites must be considered when MCs are counted for a reliable diagnosis of mastocytosis. A pilot study in patients with mastocytosis underlined the variation in the numbers of MCs in mastocytosis and normal skin, but showed a considerable overlap. The observed numbers of MCs in adults cannot be extrapolated to children.
  
  
  CONCLUSIONS
  MC numbers varied significantly between proximal and distal body sites and these differences must be considered when MCs are counted for a reliable diagnosis of mastocytosis. There was a considerable overlap between the numbers of MCs in mastocytosis and normal skin.
authors.affiliation: Department of Dermatology and Venereology, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands.
authors.forename: A S
authors.initials: AS
authors.lastname: Janssens
authors.forename: R
authors.initials: R
authors.lastname: Heide
authors.forename: J C
authors.initials: JC
authors.lastname: den Hollander
authors.forename: P G M
authors.initials: PG
authors.lastname: Mulder
authors.forename: B
authors.initials: B
authors.lastname: Tank
authors.forename: A P
authors.initials: AP
authors.lastname: Oranje
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001132:Arm / Q000033:anatomy & histology; D002196:Capillaries / Q000033:anatomy & histology; D002452:Cell Count; D006801:Humans; D007866:Leg / Q000033:anatomy & histology; D008407:Mast Cells / Q000166:cytology*; D034701:Mastocytosis, Cutaneous / Q000473:pathology; D008875:Middle Aged; D010865:Pilot Projects; D012016:Reference Values; D012867:Skin / Q000098:blood supply / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods
references.citation: Am J Dermatopathol. 1987 Jun;9(3):208-11
references.pmid: 3631448
references.citation: J Cutan Pathol. 1995 Apr;22(2):154-9
references.pmid: 7560349
references.citation: Br J Dermatol. 1987 Jul;117(1):29-36
references.pmid: 2443157
references.citation: Immunol Rev. 2001 Feb;179:74-81
references.pmid: 11292030
references.citation: Int Arch Allergy Immunol. 2002 Feb;127(2):143-6
references.pmid: 11919426
references.citation: Int Arch Allergy Immunol. 2002 Feb;127(2):147-52
references.pmid: 11919427
references.citation: Curr Opin Allergy Clin Immunol. 2001 Oct;1(5):449-54
references.pmid: 11964726
references.citation: Br J Dermatol. 2003 Feb;148(2):224-8
references.pmid: 12588371
references.citation: Clin Exp Dermatol. 1976 Dec;1(4):313-21
references.pmid: 793744
references.citation: Br J Dermatol. 1979 Jun;100(6):623-33
references.pmid: 465310
references.citation: Br J Dermatol. 1979 Jun;100(6):635-40
references.pmid: 465311
references.citation: Am J Pathol. 1985 Apr;119(1):57-64
references.pmid: 3985123
references.citation: J Am Acad Dermatol. 1987 Feb;16(2 Pt 1):326-31
references.pmid: 3819067
references.citation: J Allergy Clin Immunol. 1988 Sep;82(3 Pt 1):425-32
references.pmid: 3170991
references.citation: J Pathol. 1990 Oct;162(2):119-26
references.pmid: 2250189
references.citation: Photodermatol Photoimmunol Photomed. 1991 Oct;8(5):195-9
references.pmid: 1822682
references.citation: J Invest Dermatol. 1994 Dec;103(6):797-800
references.pmid: 7798617
references.citation: Dermatology. 1995;190(1):9-13
references.pmid: 7894111
references.citation: J Am Acad Dermatol. 1995 Sep;33(3):514-7
references.pmid: 7657877
references.citation: Photochem Photobiol. 1996 Jan;63(1):123-7
references.pmid: 8577864
references.citation: Acta Anat (Basel). 1950;10(1-2):81-107
references.pmid: 14777248
references.citation: J Invest Dermatol. 1964 Oct;43:249-54
references.pmid: 14220232
references.citation: Arch Dermatol. 1987 Aug;123(8):1017-21
references.pmid: 2443079