title: Glutathione S-transferase tissue profiling by reporter peptide monitoring.
abstract: Glutathione S-transferases (GSTs) form a widespread enzyme superfamily mainly involved in phase II detoxification. Differential expression of the various GST isoforms, differing in catalytic and structural properties, correlates with physiological and pathological states. Fast and simple determination of the GST profile is expected to be an important diagnostic tool in disease analysis. Here we propose a combined approach of high resolution separation techniques and electrospray mass spectrometric analyses for characterizing the spectrum of GSTs in male mouse liver. In this approach, the sensitivity and speed required for tissue GST profiling studies is achieved by tracking the reconstructed ion current of selected reporter peptides following chromatographic separation. This simple procedure, in which an affinity protein bait is followed by a chemical fragmentation and mass spectrometric analysis, could be sufficiently sensitive to detect the qualitative differences between physiological and pathological states.
authors.affiliation: Dipartimento di Scienze Biochimiche, Universitá La Sapienza, Rome, Italy. eugenia.schinina@uniroma1.it
authors.forename: M Eugenia
authors.initials: ME
authors.lastname: Schininà
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Pitari
authors.forename: Tommaso
authors.initials: T
authors.lastname: Paolone
authors.forename: Alessandra
authors.initials: A
authors.lastname: Giorgi
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Ippoliti
authors.forename: Silvestro
authors.initials: S
authors.lastname: Duprè
authors.forename: Bruno
authors.initials: B
authors.lastname: Maras
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005982:Glutathione Transferase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis; D008099:Liver / Q000737:chemistry*; D008297:Male; D051379:Mice; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007527:Isoenzymes; D010455:Peptides; D005982:Glutathione Transferase