Relationship between physical, biochemical and biomechanical properties of tissue-engineered cartilage-carrier-constructs.
Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. Physical parameters (height and weight) correlated very well with total DNA content (r2 = 0.86, re. 0.94). The relation between DNA content and glycosaminoglycan content was less but still significant. No significant relationship was found between the elasticity module and the DNA content, even if the elasticity module increased slightly at higher DNA content. With respect to later implantation, selection of a construct for implantation based on the weight, which can be determined non-invasive and under sterile conditions, seems to be justifiable.
Nagel-Heyer
Stephanie
S
Bioprocess and Biochemical Engineering, Hamburg, Germany.
Goepfert
Christiane
C
Morlock
Michael M
MM
Pörtner
Ralf
R
D000818:Animals; D001696:Biomechanical Phenomena; D002356:Cartilage / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000166:cytology; D004247:DNA / Q000032:analysis; D004548:Elasticity; D006025:Glycosaminoglycans / Q000032:analysis; D012044:Regression Analysis; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D006025:Glycosaminoglycans; D004247:DNA