title: Viability and adipogenic potential of human adipose tissue processed cell population obtained from pump-assisted and syringe-assisted liposuction.
abstract: BACKGROUND
  Correcting soft tissue defects by autologous fat grafting has been a routine procedure in plastic surgery. Liposuction material for autologous fat grafting is normally obtained using a hand-held syringe. The pump-assisted liposuction technique is not used because of the belief that cells in the harvested fat tissue are necrotic from the physical forces created by the vacuum pump liposuction machine.
  
  
  OBJECTIVE
  The metabolism and adipogenic potential of cultured mesenchymal precursor cells processed from pump and syringe lipoaspirates were studied.
  
  
  METHODS
  Metabolic estimates were determined with alamarBlue assay. These cells were also induced along the adipogenic lineage with adipogenic induction factors. The extent of adipogenic differentiation was measured using a digital photo counting software.
  
  
  RESULTS
  The cells obtained by pump-assisted liposuction are viable, have comparable metabolic activity and adipogenic potential which are comparable to cells using the syringe for aspiration.
  
  
  CONCLUSIONS
  The implications of this study are that pump-assisted liposuction might be a possible alternative to aspirating adipose tissue for reimplantation during reconstruction procedures.
authors.affiliation: Department of Biological Science, National University of Singapore, Singapore.
authors.forename: David T W
authors.initials: DT
authors.lastname: Leong
authors.forename: Dietmar W
authors.initials: DW
authors.lastname: Hutmacher
authors.forename: F T
authors.initials: FT
authors.lastname: Chew
authors.forename: Thiam-Chye
authors.initials: TC
authors.lastname: Lim
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000473:pathology; D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology*; D001391:Azo Compounds / Q000494:pharmacology; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002470:Cell Survival; D004396:Coloring Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D006801:Humans; D015187:Lipectomy / Q000295:instrumentation* / Q000379:methods*; D050356:Lipid Metabolism; D008648:Mesoderm / Q000378:metabolism; D010078:Oxazines / Q000494:pharmacology; D013396:Suction; D013594:Syringes; D013997:Time Factors; D014966:Xanthenes / Q000494:pharmacology
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D003470:Culture Media; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; C011049:oil red O