Impedimetric immunosensors based on the conjugated polymer PPV.
In the work reported here, we investigated the interaction between the semiconducting polymer MDMO-PPV and antibodies against the fluorescent dyes fluorescein isothiocyanate (FITC) and Cy5. The antibodies are adsorbed physically onto thin polymer films on gold electrodes, as seen in AFM images of these films. By tuning the antibody concentration, the contact angle of distilled water with the film can be made to vary between 95 degrees and 50 degrees, showing that different surface densities of antibody can be obtained. That these biosensor films specifically bind their antigenic fluorescent molecules from PBS buffer solution is demonstrated by confocal fluorescence microscopy. Specific antigen-antibody recognition is demonstrated by lack of cross-sensitivity between the two antibodies and their antigens. In a biosensor prototype based on differential impedance spectroscopy, these polymer films show a clear response to 1 ppb antigen solution, with a time constant of 2-3 min.
Cooreman
P
P
Institute for Materials Research, Limburgs Universitair Centrum, Wetenschapspark 1, B-3590 Diepenbeek, Belgium. peter.cooreman@luc.ac.be
Thoelen
R
R
Manca
J
J
vandeVen
M
M
Vermeeren
V
V
Michiels
L
L
Ameloot
M
M
Wagner
P
P
D000941:Antigens / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D011145:Polyvinyls / Q000737:chemistry*; D012666:Semiconductors
D000941:Antigens; D020099:Coated Materials, Biocompatible; D011145:Polyvinyls; C470024:poly(4-phenylenevinylene)