The protein folding transition state: what are Phi-values really telling us?
Protein engineering-based studies of the folding transition state have accelerated significantly in the last decade, and more than a half dozen proteins have been subjected to extensive Phi-value analysis. A general picture is emerging from these studies of a transition state in which the large majority of experimentally characterized side chains participate in relatively homogeneous and energetically weak interactions playing only a relatively small role in defining relative folding rates.
Raleigh
Daniel P
DP
Department of Chemistry, Graduate Program in Biochemistry and Structural Biology, State University of New York at Stony Brook, NY 11794-3400, USA.
Plaxco
Kevin W
KW
D011487:Protein Conformation; D015202:Protein Engineering; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry / Q000235:genetics
D011506:Proteins