title: eNOS genotype is without effect on circulating nitrite/nitrate level in healthy male population.
abstract: BACKGROUND
  Nitric oxide (NO) plays an important role in the regulation of the cardiovascular system. It is produced by endothelial nitric oxide synthase (eNOS), which exhibits genetic polymorphisms. Although the clinically relevant polymorphism T(+IhI-786)C reduces eNOS-promoter activity, it is not clear whether circulating nitrite/nitrate (NOx) are affected by this polymorphism.
  
  
  METHODS
  We addressed this issue by studying a homogeneous group of 200 healthy subjects (males, Caucasians, nonsmokers, 18+IBM-56 years of age, and not taking any medication). Genotypes were determined by restriction fragment length polymorphism and circulating NOx were determined by chemiluminescence.
  
  
  RESULTS
  We found nonsignificant effects of the T(+IhI-786)C polymorphism on circulating NOx (mean+ALE-S.D.=52.2+ALE-21.4, 49.0+ALE-17.8, and 45.9+ALE-16.8 +A7w-mol/L for genotypes +IBw-TT,+IB0 +IBw-TC,+IB0 and +IBw-CC,+IB0 respectively) and on total plasma cholesterol concentrations (both P>.05). No correlation was found between circulating NOx and total plasma cholesterol concentrations (P>.05).
  
  
  CONCLUSIONS
  Our study provides strong evidence that the T(+IhI-786)C polymorphism does not affect plasma NOx concentrations, which are believed to reflect endogenous production of NO. Therefore, our results suggest that this polymorphism does not affect endogenous NO production.
authors.affiliation: Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Nagassaki
authors.forename: Ingrid F
authors.initials: IF
authors.lastname: Metzger
authors.forename: Debora C
authors.initials: DC
authors.lastname: Souza-Costa
authors.forename: Aline S
authors.initials: AS
authors.lastname: Marroni
authors.forename: Juliana A
authors.initials: JA
authors.lastname: Uzuelli
authors.forename: Jose E
authors.initials: JE
authors.lastname: Tanus-Santos
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D005838:Genotype; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000097:blood*; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D009573:Nitrites / Q000097:blood*; D011110:Polymorphism, Genetic / Q000235:genetics; D011153:Population / Q000235:genetics*; D012016:Reference Values; D012737:Sex Factors; D044465:White People / Q000235:genetics*
chemical_list: D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III