Following the formation of supported lipid bilayers on mica: a study combining AFM, QCM-D, and ellipsometry.
Supported lipid bilayers (SLBs) are popular models of cell membranes with potential biotechnological applications and an understanding of the mechanisms of SLB formation is now emerging. Here we characterize, by combining atomic force microscopy, quartz crystal microbalance with dissipation monitoring, and ellipsometry, the formation of SLBs on mica from sonicated unilamellar vesicles using mixtures of zwitterionic, negatively and positively charged lipids. The results are compared with those we reported previously on silica. As on silica, electrostatic interactions were found to determine the pathway of lipid deposition. However, fundamental differences in the stability of surface-bound vesicles and the mobility of SLB patches were observed, and point out the determining role of the solid support in the SLB-formation process. The presence of calcium was found to have a much more pronounced influence on the lipid deposition process on mica than on silica. Our results indicate a specific calcium-mediated interaction between dioleoylphosphatidylserine molecules and mica. In addition, we show that the use of PLL-g-PEG modified tips considerably improves the AFM imaging of surface-bound vesicles and bilayer patches and evaluate the effects of the AFM tip on the apparent size and shape of these soft structures.
Richter
Ralf P
RP
Laboratoire d'Imagerie Moléculaire et Nano-Bio-Technologie, IECB, UMR-CNRS 5471, Université Bordeaux I, 33607 Pessac Cedex, France.
Brisson
Alain R
AR
D000538:Aluminum Silicates / Q000737:chemistry*; D001703:Biophysics / Q000379:methods*; D001709:Biotechnology / Q000379:methods; D002118:Calcium / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D004492:Edetic Acid / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors
D000538:Aluminum Silicates; D005229:Fatty Acids, Monounsaturated; D008051:Lipid Bilayers; D008055:Lipids; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols; C042511:1,2-dioleoylphosphatidylserine; D012822:Silicon Dioxide; D004492:Edetic Acid; C017251:1,2-oleoylphosphatidylcholine; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; D002118:Calcium; C011934:mica; D012825:Silicon
Ultramicroscopy. 2003 Oct-Nov;97(1-4):217-27
12801674
Biophys J. 1998 Sep;75(3):1397-1402
9726940
Curr Opin Chem Biol. 2000 Dec;4(6):704-9
11102877
Science. 1996 Jan 5;271(5245):43-8
8539599
Langmuir. 2004 May 25;20(11):4609-13
15969172
Nature. 1996 Jan 18;379(6562):219-25
8538786
Biophys J. 2000 Dec;79(6):3153-63
11106620
J Biol Chem. 1983 Feb 25;258(4):2426-31
6822569
Biochemistry. 1996 Nov 26;35(47):14773-81
8942639
FEBS Lett. 1994 Feb 28;340(1-2):121-3
8119394
Biophys J. 1990 Aug;58(2):357-62
2207243
Phys Rev Lett. 2000 Jun 5;84(23):5443-6
10990964
Biophys J. 1991 Feb;59(2):289-94
2009353
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7564-8
6334313
J Struct Biol. 1998;121(3):356-61
9705879
J Colloid Interface Sci. 1997 Jul 1;191(1):102-9
9241209
Phys Rev E Stat Nonlin Soft Matter Phys. 2002 Nov;66(5 Pt 1):051905
12513521
EMBO Rep. 2002 May;3(5):485-90
11964385
Biochim Biophys Acta. 1986 Jun 12;864(1):95-106
2941079
Biophys J. 2002 Dec;83(6):3371-9
12496104
Anal Chem. 2003 Oct 1;75(19):5080-7
14708781
Langmuir. 2004 Nov 9;20(23):10129-37
15518504
Biophys J. 2003 Nov;85(5):3035-47
14581204
Langmuir. 2005 Jan 4;21(1):299-304
15620318
Phys Rev A. 1990 Oct 15;42(8):4768-4771
9904586
J Mol Biol. 2002 Apr 19;318(1):97-107
12054771
Biophys J. 1995 Oct;69(4):1447-55
8534815
Colloids Surf B Biointerfaces. 2004 Mar 1;34(1):41-51
15261089
Biol Chem. 2002 Feb;383(2):337-41
11934273