[Rapid determination of aspartame in compound sweetening by reversed-phase high performance liquid chromatography].
A method for rapid determination of Aspartame in compound sweetening by reversed-phase high performance liquid chromatography is presented. Aspartame in compound sweetening was separated in a short column (Ultrasphere XL-ODS, 3 microm, 4.6 mm x 70 mm) by using CH3OH-0.02 mol/L NH4Ac as mobile phase. The flow rate was 0.8 mL/min. Detection was performed with UV detector at 220 nm. The injection volume was 20 microL. It was qualitatively analysed by UV scanning at a wavelength range of 200-350 nm under no-stop flow according to their retention time. Quantitative analysis was carried out by measuring peak height and comparing it with external standard. The minimum detectable amount was 5 microg/L. The linear range of the calibration curve was 40-200 mg/L. The average recovery of Aspartame was 92%. The relative standard deviation was 2.9%. This method is simple, rapid and sensitive.
Zhang
R
R
Central Laboratory, Zhejiang Sanitation and Antiepidemic Station, Hangzhou, 310009.
Jiang
M
M
D001218:Aspartame / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013549:Sweetening Agents / Q000737:chemistry*
D013549:Sweetening Agents; D001218:Aspartame