Deconstructing PTI-1: PTI-1 is a truncated, but not mutated, form of translation elongatin factor 1A1, eEF1A1.
The prostate tumor-inducing gene 1 (PTI-1) transcript is detected in various human carcinoma cells. PTI-1 is reported to consist of a 5' untranslated region (5' UTR) homologous to mycoplasma 23S rRNA and a coding region corresponding to a truncated and mutated form of the translation elongation factor 1A, eEF1A. We have found that the PTI-1 transcript may encode a truncated, but not mutated, form of the human isoform eEF1A1. Additionally, the 5' UTR sequence of PTI-1 from genomic DNA of different cell lines and blood samples varies from the original sequence. This 5' -UTR region of PTI-1 presents a fusion of E. coli and Mycoplasma hyorhinis 23S rRNA. We have overexpressed the potential PTI-1 protein in E. coli and various human cell lines. The resulting protein could be detected by western blotting using anti-eEF1A antibodies. However, we were unable to detect the PTI-1 protein in LNCaP cell extracts. The potential roles of the PTI-1 protein in carcinogenesis and the origin of the PTI-1 gene in the human genome are discussed.
Mansilla
Francisco
F
Department of Molecular Biology, University of Aarhus, Arhus C, Denmark. crk@imsb.au.dk
Hansen
Lise Lotte
LL
Jakobsen
Helle
H
Kjeldgaard
Niels Ole
NO
Clark
Brian F C
BF
Knudsen
Charlotte R
CR
D001483:Base Sequence; D045744:Cell Line, Tumor; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D009174:Mycoplasma / Q000235:genetics; D015513:Oncogene Proteins / Q000235:genetics*; D010441:Peptide Chain Elongation, Translational / Q000235:genetics*; D020648:Peptide Elongation Factor 1; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics*
D017931:DNA Primers; C094814:EEF1A1 protein, human; D015513:Oncogene Proteins; D020648:Peptide Elongation Factor 1