title: Multitarget FISH analysis in the diagnosis of lung cancer.
abstract: The aim of the present study was to explore the diagnostic usefulness of the multitarget fluorescence in situ hybridization (FISH) test, LAVysion (Vysis, Downers Grove, IL), for the detection of lung cancer cells in cytologic specimens. Specimens from bronchial washings, bronchial brushings, and transbronchial fine-needle aspirates (TBNAs) from 100 patients with suspected lung cancer and from a control group of 71 patients with nonneoplastic lung disorders were analyzed. FISH positivity was defined as more than 5 cells with gains of at least 2 chromosomes or gene loci. FISH significantly improved the sensitivity of bronchial brushings from 49% to 73%. The specificities of FISH and cytologic examination were 87% and 100%, respectively. In contrast, FISH provided no additional diagnostic information in TBNAs and bronchial washings. There was no increased prevalence of genetic changes in contralateral bronchial washings from patients with lung cancer compared with the control group. The quantity of previous smoking had no effect on the prevalence of chromosomal changes.
authors.affiliation: Institute for Pathology, University Hospital, Basel, Switzerland.
authors.forename: Lukas
authors.initials: L
authors.lastname: Bubendorf
authors.forename: Phaedra
authors.initials: P
authors.lastname: Müller
authors.forename: Ladina
authors.initials: L
authors.lastname: Joos
authors.forename: Bruno
authors.initials: B
authors.lastname: Grilli
authors.forename: Sandrine
authors.initials: S
authors.lastname: Vogel
authors.forename: Michelle
authors.initials: M
authors.lastname: Herzog
authors.forename: Audrey
authors.initials: A
authors.lastname: Barascud
authors.forename: Georg
authors.initials: G
authors.lastname: Feichter
authors.forename: Peter
authors.initials: P
authors.lastname: Dalquen
authors.forename: Michael
authors.initials: M
authors.lastname: Tamm
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012907:Smoking