title: Glutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid.
abstract: Glutathione S-transferases (GST) in insects play an important role in the detoxification of many substances including allelochemicals from plants. Induction of GST activity in Myzus persicae in response to secondary metabolites from Brassica plants was determined using different host plant species and confirmed using artificial diet with pure allelochemicals added. The 2,4-dinitro-1-iodobenzene (DNIB) was found to be a useful substrate for identifying particular GSTs in insects. GSTs from M. persicae were purified using different affinity chromatography columns and related kinetic parameters were calculated. GST isoenzymes were characterised using electrophoretic methods. Although SDS-PAGE results indicated similarity among the purified enzymes from each affinity column, biochemical studies indicated significant differences in kinetic parameters. Finally, the GST pattern of M. persicae was discussed in terms of insect adaptation to the presence of plant secondary substances such as the glucosinolates and the isothiocyanates, from Brassicaceae host plants.
authors.affiliation: Department of Pure and Applied Zoology, Gembloux Agricultural University, Gembloux, Belgium. francis.f@fsagx.ac.be
authors.forename: Frédéric
authors.initials: F
authors.lastname: Francis
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vanhaelen
authors.forename: Eric
authors.initials: E
authors.lastname: Haubruge
mesh_terms: D000222:Adaptation, Physiological; D000704:Analysis of Variance; D000818:Animals; D001042:Aphids / Q000187:drug effects / Q000201:enzymology*; D001937:Brassica / Q000737:chemistry*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000378:metabolism*; D007462:Iodobenzenes; D007700:Kinetics; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology
chemical_list: D007462:Iodobenzenes; D010936:Plant Extracts; D005982:Glutathione Transferase