Water deficits affect caffeate O-methyltransferase, lignification, and related enzymes in maize leaves. A proteomic investigation.
Drought is a major abiotic stress affecting all levels of plant organization and, in particular, leaf elongation. Several experiments were designed to study the effect of water deficits on maize (Zea mays) leaves at the protein level by taking into account the reduction of leaf elongation. Proteomic analyses of growing maize leaves allowed us to show that two isoforms of caffeic acid/5-hydroxyferulic 3-O-methyltransferase (COMT) accumulated mostly at 10 to 20 cm from the leaf point of insertion and that drought resulted in a shift of this region of maximal accumulation toward basal regions. We showed that this shift was due to the combined effect of reductions in growth and in total amounts of COMT. Several other enzymes involved in lignin and/or flavonoid synthesis (caffeoyl-CoA 3-O-methyltransferase, phenylalanine ammonia lyase, methylenetetrahydrofolate reductase, and several isoforms of S-adenosyl-l-methionine synthase and methionine synthase) were highly correlated with COMT, reinforcing the hypothesis that the zone of maximal accumulation corresponds to a zone of lignification. According to the accumulation profiles of the enzymes, lignification increases in leaves of control plants when their growth decreases before reaching their final size. Lignin levels analyzed by thioacidolysis confirmed that lignin is synthesized in the region where we observed the maximal accumulation of these enzymes. Consistent with the levels of these enzymes, we found that the lignin level was lower in leaves of plants subjected to water deficit than in those of well-watered plants.
Vincent
Delphine
D
Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université Paris XI, Institut National Agronomique Paris-Grignon, la Ferme du Moulon, 91190 Gif-sur-Yvette, France.
Lapierre
Catherine
C
Pollet
Brigitte
B
Cornic
Gabriel
G
Negroni
Luc
L
Zivy
Michel
M
D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000378:metabolism; D008031:Lignin / Q000096:biosynthesis*; D008780:Methyltransferases / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics; D013997:Time Factors; D014867:Water / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology*
D007527:Isoenzymes; D010940:Plant Proteins; D014867:Water; D008031:Lignin; D008780:Methyltransferases; C019608:caffeate O-methyltransferase
Plant Cell. 1989 Jan;1(1):81-93
2535470
Phytochemistry. 2002 Oct;61(3):221-94
12359514
J Exp Bot. 2000 Sep;51(350):1505-14
11006302
Plant Physiol. 1993 Oct;103(2):509-17
7518088
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:585-609
15012247
Plant Physiol. 1998 Jul;117(3):761-70
9662519
Crit Rev Biochem Mol Biol. 2003;38(4):305-50
14551235
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8955-60
10430877
Electrophoresis. 1993 Oct;14(10):1067-73
8125057
Plant J. 2000 Jun;22(5):415-26
10849357
Curr Opin Plant Biol. 2002 Jun;5(3):224-9
11960740
Plant Physiol. 1998 Aug;117(4):1253-63
9701581
Annu Rev Plant Biol. 2003;54:519-46
14503002
Plant Cell. 1995 Jul;7(7):1085-1097
12242399
Plant Physiol. 2002 Dec;130(4):1675-85
12481050
Plant Physiol. 1995 Nov;109(3):861-870
12228638
Plant Mol Biol. 2001 Sep;47(1-2):275-91
11554476
Plant Physiol. 1993 Jul;102(3):1001-8
8278520
J Biol Chem. 1999 Dec 17;274(51):36089-96
10593891
J Biol Chem. 1975 May 25;250(10):4007-21
236308
Funct Plant Biol. 2002 Jan;29(1):55-61
32689451
Plant Mol Biol. 1992 Dec;20(5):857-67
1463825
Plant Physiol. 1996 Jan;110(1):3-13
12226169
Physiol Plant. 2002 Oct;116(2):238-247
12354201
Plant Mol Biol. 1994 May;25(2):217-27
8018871
Anal Biochem. 1976 May 7;72:248-54
942051
C R Acad Sci III. 2001 Sep;324(9):863-8
11558333
Arch Biochem Biophys. 1989 Jun;271(2):488-94
2499260
New Phytol. 1995 Feb;129(2):203-236
33874561
Plant Cell. 2002 Jun;14(6):1265-77
12084826
Plant Mol Biol. 2003 Jun;52(3):495-509
12956522
Nat Struct Biol. 2001 Mar;8(3):271-9
11224575
Plant Mol Biol. 1998 Nov 1;38(4):513-20
9747797
J Biol Chem. 2000 Mar 3;275(9):6537-45
10692459
Arch Biochem Biophys. 2000 Mar 1;375(1):175-82
10683265
Plant Physiol. 1997 Jul;114(3):893-900
12223750
J Biol Chem. 1991 Sep 15;266(26):17416-23
1894629
Annu Rev Plant Physiol Plant Mol Biol. 1990;41:455-96
11543592
Planta. 2000 Mar;210(4):589-98
10787052
Plant J. 2001 Jan;25(2):193-202
11169195
Plant Mol Biol. 1998 Nov 1;38(4):587-96
9747804
Plant Mol Biol. 1994 Dec;26(6):1797-806
7858218
Plant Cell. 1995 Apr;7(4):407-16
7773015
Plant Physiol. 1997 Mar;113(3):967-973
12223657
Planta. 2002 Sep;215(5):785-93
12244444
Ann Bot. 2002 Jan;89(1):89-96
12096823
J Agric Food Chem. 2000 Dec;48(12):6106-13
11312783
Plant Physiol. 1991 Sep;97(1):7-14
16668418
Plant Physiol. 1996 Oct;112(2):717-726
12226420
Plant Physiol. 1997 Jul;114(3):1123-33
9232887
Plant Physiol. 1999 Sep;121(1):215-24
10482677