Movies, measurement, and modeling: the three Ms of mechanistic immunology.
Immunological phenomena that were once deduced from genetic, biochemical, and in situ approaches are now being witnessed in living color, in three dimensions, and in real time. The information in time-lapse imaging can provide valuable mechanistic insight into a host of processes, from cell migration to signal transduction. What we need now are methods to quantitate these new visual data and to exploit computational resources and statistical mechanical methods to develop mechanistic models.
Witt
Colleen
C
University of California, Berkeley, CA 94720, USA.
Raychaudhuri
Subhadip
S
Chakraborty
Arup K
AK
D000486:Allergy and Immunology / Q000639:trends*; D000818:Animals; D019295:Computational Biology; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence; D018448:Models, Immunological; D012984:Software; D013995:Time; D014741:Video Recording
Semin Immunol. 2000 Feb;12(1):5-21
10723794
PLoS Biol. 2005 Jun;3(6):e160
15869324
Science. 2002 Feb 22;295(5559):1539-42
11859198
Science. 2002 Jun 7;296(5574):1869-73
12016203
Science. 2002 Jun 7;296(5574):1873-6
12052961
Science. 2002 Jun 7;296(5574):1876-80
12052962
Nature. 2002 Oct 24;419(6909):845-9
12397360
Science. 2002 Oct 25;298(5594):785-9
12399580
Nat Rev Immunol. 2002 Nov;2(11):872-80
12415310
J Immunol. 2003 Jan 1;170(1):64-72
12496384
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2604-9
12601158
Nat Immunol. 2003 Oct;4(10):933-6
14515121
Science. 2003 Nov 14;302(5648):1218-22
14512504
Nature. 2004 Jan 8;427(6970):154-9
14712275
Semin Immunol. 2003 Dec;15(6):307-15
15001169
Nat Rev Immunol. 2004 Jun;4(6):445-56
15173833
Nat Immunol. 2004 Aug;5(8):791-9
15247914
Nature. 1990 Aug 9;346(6284):574-6
2115981
Science. 1990 Aug 31;249(4972):1028-30
2118680
Science. 1996 Mar 15;271(5255):1582-6
8599114
Eur J Immunol. 1997 Dec;27(12):3310-7
9464819
Nature. 1998 Sep 3;395(6697):82-6
9738502
Science. 1999 Jul 9;285(5425):221-7
10398592
Nature. 1953 Apr 25;171(4356):737-8
13054692
J Immunol. 2000 Jun 1;164(11):5626-34
10820237