Clinical investigation: increased serum stromal derived factor 1 alpha levels in pulmonary tuberculosis.
Pulmonary tuberculosis, a granulomatous disease, has few serological markers for its activity. Recently, an increased plasma level of stromal derived factor 1 alpha (SDF-1alpha), which can induce strong chemotaxis of cells through its receptor CXCR4, was detected in patients with tuberculosis. In this study we investigated serum SDF-1alpha levels and CXCR4 expression on peripheral blood mononuclear cells (PBMCs). Fifty-five active tuberculosis patients, 30 resolved tuberculosis patients, 27 acute bronchitis patients and 8 healthy volunteers were examined. Histological expression of SDF-1alpha in the tuberculosis lesion and CXCR4 expression of PBMCs were also analysed. Serum SDF-1alpha levels in active tuberculosis patients were significantly higher than other groups. The sensitivity and specificity for the diagnosis of active tuberculosis was 88.5% and 85.3% (cutoff value = 650 pg/ml), respectively. CXCR4 expression levels on PBMCs showed a significant negative correlation with serum SDF-1alpha levels. Inflammatory cells including multinuclear giant cells in the lesion expressed SDF-1alpha. Measurement of serum SDF-1alpha could be a useful screening marker for the identification of active pulmonary tuberuculosis. We propose that interaction of SDF-1alpha and CXCR4 might be involved in the pathogenesis of pulmonary tuberculosis.
Mizuno
K
K
Third Department of Internal Medicine, Kagoshima University Faculty of Medicine, Kagoshima, Japan.
Matsuyama
W
W
Mitsuyama
H
H
Watanabe
M
M
Higashimoto
I
I
Osame
M
M
Arimura
K
K
D000208:Acute Disease; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D001991:Bronchitis / Q000276:immunology; D016022:Case-Control Studies; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000032:analysis / Q000097:blood*; D018432:Drug Resistance, Multiple; D005260:Female; D005434:Flow Cytometry; D015726:Giant Cells / Q000737:chemistry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D019718:Receptors, CXCR4 / Q000032:analysis; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000188:drug therapy / Q000276:immunology
D015415:Biomarkers; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D019718:Receptors, CXCR4
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