title: DNA polymerase V and RecA protein, a minimal mutasome.
abstract: A hallmark of the Escherichia coli SOS response is the large increase in mutations caused by translesion synthesis (TLS). TLS requires DNA polymerase V (UmuD'2C) and RecA. Here, we show that pol V and RecA interact by two distinct mechanisms. First, pol V binds to RecA in the absence of DNA and ATP and second, through its UmuD' subunit, requiring DNA and ATP without ATP hydrolysis. TLS occurs in the absence of a RecA nucleoprotein filament but is inhibited in its presence. Therefore, a RecA nucleoprotein filament is unlikely to be required for SOS mutagenesis. Pol V activity is severely diminished in the absence of RecA or in the presence of RecA1730, a mutant defective for pol V mutagenesis in vivo. Pol V activity is strongly enhanced with RecA mutants constitutive for mutagenesis in vivo, suggesting that RecA is an obligate accessory factor that activates pol V for SOS mutagenesis.
authors.affiliation: Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
authors.forename: Katharina
authors.initials: K
authors.lastname: Schlacher
authors.forename: Kris
authors.initials: K
authors.lastname: Leslie
authors.forename: Claire
authors.initials: C
authors.lastname: Wyman
authors.forename: Roger
authors.initials: R
authors.lastname: Woodgate
authors.forename: Michael M
authors.initials: MM
authors.lastname: Cox
authors.forename: Myron F
authors.initials: MF
authors.lastname: Goodman
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D004249:DNA Damage; D004261:DNA Replication / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000378:metabolism; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics*; D011938:Rec A Recombinases / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D000255:Adenosine Triphosphate; D011938:Rec A Recombinases; C120926:DNA polymerase V, E coli; D004259:DNA-Directed DNA Polymerase; C055218:UmuD protein, E coli