title: Induction and characterization of streptomycin-resistant mutants in Capsicum praetermissum.
abstract: Streptomycin-resistant mutants were isolated from mutagenised cotyledon explants of Capsicum praetermissum Heiser & Smith. The explants were mutagenised with N-ethyl-N-nitrosourea, which resulted in a high frequency of streptomycin-resistant mutants (18.0%) and a low frequency of chlorophyll-deficient (albino) mutants (8.0%). Complete streptomycin-resistant plantlets were obtained after rooting of the regenerated green shoots on rooting medium containing 1.0 mg L-1 IAA and 500 mg L-1 streptomycin sulphate. Leaf-segment assay of these plantlets revealed that they were resistant to streptomycin but sensitive to chloramphenicol, kanamycin, lincomycin, and spectinomycin. Reciprocal crosses between streptomycin-resistant and -sensitive plants showed a non-Mendelian transmission of resistance by female parents.
authors.affiliation: Plant Biotechnology Laboratory, Department of Botany, Kakatiya University, Warangal-506 009 A P, India. peddaboina@hotmail.com
authors.forename: Peddaboina
authors.initials: P
authors.lastname: Venkataiah
authors.forename: Thamidala
authors.initials: T
authors.lastname: Christopher
authors.forename: Karampuri
authors.initials: K
authors.lastname: Subhash
mesh_terms: D002212:Capsicum / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics*; D009154:Mutation; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D013307:Streptomycin