Sepharose-bound, highly sulfated glycosaminoglycans can capture HIV-1 from culture medium.
In the search for new strategies against HIV-1 and on the basis of a number of previous studies reporting on the capacity of certain polyanionic compounds to influence the replication of HIV-1, we prepared a few chemically oversulfated dermatan and chondroitin sulfates. Four of these compounds and two samples of heparin were bound to activated Sepharose through either their carboxylic groups, or their aldehydic groups, or their deacetylated primary amino groups. Some of these so-derivatised resins, packed into columns, proved able to remove HIV-1 IIIB, a laboratory adapted strain, and one clinical primary isolate from an AIDS patient, from infected cell culture medium. The resins bind the virus very tightly and could be useful for capturing the virus from infected fluids.
Curatella
Barbara
B
Istituto di Cristallografia, Sezione di Monterotondo, CNR, PO Box 10, I-00016 Monterotondo Stazione, Rome, Italy.
Bartolini
Barbara
B
Di Caro
Antonino
A
Cavallaro
Rosaria A
RA
Liverani
Lino
L
Mascellani
Giuseppe
G
Benedetto
Arrigo
A
Castilletti
Concetta
C
Capobianchi
Maria R
MR
Cellai
Luciano
L
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology; D003470:Culture Media; D003871:Dermatan Sulfate / Q000737:chemistry / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D012685:Sepharose / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000378:metabolism*
D003470:Culture Media; D006025:Glycosaminoglycans; D013431:Sulfates; D003871:Dermatan Sulfate; D006493:Heparin; D002809:Chondroitin Sulfates; D012685:Sepharose