Membrane-based on-line optical analysis system for rapid detection of bacteria and spores.
We report here the adaptation of our electronic microchip technology towards the development of a new method for detecting and enumerating bacterial cells and spores. This new approach is based on the immuno-localization of bacterial spores captured on a membrane filter microchip placed within a flow cell. A combination of microfluidic, optical, and software components enables the integration of staining of the bacterial species with fully automated assays. The quantitation of the analyte signal is achieved through the measurement of a collective response or alternatively through the identification and counting of individual spores and particles. This new instrument displays outstanding analytical characteristics, and presents a limit of detection of approximately 500 spores when tested with Bacillus globigii (Bg), a commonly used simulant for Bacillus anthracis (Ba), with a total analysis time of only 5 min. Additionally, the system performed well when tested with real postal dust samples spiked with Bg in the presence of other common contaminants. This new approach is highly customizable towards a large number of relevant toxic chemicals, environmental factors, and analytes of relevance to clinical chemistry applications.
Floriano
Pierre N
PN
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, USA.
Christodoulides
Nick
N
Romanovicz
Dwight
D
Bernard
Bruce
B
Simmons
Glennon W
GW
Cavell
Myles
M
McDevitt
John T
JT
D001407:Bacillus / Q000166:cytology / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009862:Online Systems; D055095:Optics and Photonics / Q000295:instrumentation; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D013170:Spores / Q000166:cytology / Q000302:isolation & purification; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods
D008567:Membranes, Artificial