title: In vivo mutagenicities of damaged nucleotides produced by nitric oxide and ionizing radiation.
abstract: To evaluate the in vivo mutagenicities of damaged DNA precursors (deoxyribonucleoside 5'-triphosphates) produced by exposure to nitric oxide (NO) and ionizing radiation, five damaged deoxyribonucleotides (deoxyxanthosine triphosphate, deoxyoxanosine triphosphate, dITP, dUTP, and 8-hydroxy-dATP) were introduced into competent Escherichia coli cells. Their mutagenic potentials were assayed using the chromosomal rpoB gene as a mutagenesis target. In contrast to 8-hydroxy-dGTP and 2-hydroxy-dATP, which were examined in an earlier study, none of these damaged deoxyribonucleotides significantly increased the rpoB mutant frequency. These results suggest that these five damaged deoxyribonucleotides are weakly mutagenic in vivo if at all. Thus their contributions to mutations induced by NO and ionizing radiation may be small.
authors.affiliation: Hokkaido University Graduate School of Pharmaceutical Sciences, Nishi-6, Sapporo, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Hori
authors.forename: Chieko
authors.initials: C
authors.lastname: Ishiguro
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
mesh_terms: D003854:Deoxyribonucleotides / Q000235:genetics* / Q000528:radiation effects*; D004926:Escherichia coli / Q000235:genetics; D005720:Gamma Rays; D016296:Mutagenesis; D009569:Nitric Oxide / Q000494:pharmacology*
chemical_list: D003854:Deoxyribonucleotides; D009569:Nitric Oxide