title: Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes.
abstract: The important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in Chlamydomonas reinhardtii suggests that transcriptional profiling of such cells would reveal new flagella-related genes. We have conducted a genome-wide analysis of RNA transcript levels during flagellar regeneration in Chlamydomonas by using maskless photolithography method-produced DNA oligonucleotide microarrays with unique probe sequences for all exons of the 19,803 predicted genes. This analysis represents previously uncharacterized whole-genome transcriptional activity profiling study in this important model organism. Analysis of strongly induced genes reveals a large set of known flagellar components and also identifies a number of important disease-related proteins as being involved with cilia and flagella, including the zebrafish polycystic kidney genes Qilin, Reptin, and Pontin, as well as the testis-expressed tubby-like protein TULP2.
authors.affiliation: Genome Research Facility, National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035, USA. vstolc@mail.arc.nasa.gov
authors.forename: Viktor
authors.initials: V
authors.lastname: Stolc
authors.forename: Manoj Pratim
authors.initials: MP
authors.lastname: Samanta
authors.forename: Waraporn
authors.initials: W
authors.lastname: Tongprasit
authors.forename: Wallace F
authors.initials: WF
authors.lastname: Marshall
mesh_terms: D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D016680:Genome, Bacterial; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics; D007690:Polycystic Kidney Diseases / Q000235:genetics*; D012038:Regeneration; D014158:Transcription, Genetic; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D005136:Eye Proteins; D009687:Nuclear Proteins; C484551:Tulp2 protein, mouse; D029961:Zebrafish Proteins; C471327:ruvbl2 protein, zebrafish
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