[Selective extraction of organotin from biological sample by supercritical fluid extraction].
The preliminary separation and concentration of organotin compounds from the matrix of biological samples is necessary, due to the very low content of organotin and the fat included in samples will largely interfere the analysis of organotins. In this work, supercritical fluid extraction (SFE) for the preliminary separation and concentration of organotin has been studied. Pure CO2 was used as mobile phase. The extractives can be used to supercritical fluid chromatographic (SFC) analysis of organotins. The model sample was prepared by adding diphenyltin (DPT) to soya powder. The effects of the pressure and temperature on SFE were investigated. A small amount of fat was also extracted together with organotins. Extract was injected into SFC system, in which a Kapcell Pak ODS column was used for separation of organotin and fat. The effect of pressure on the extraction percentage of DPT and fat was investigated at 40 degrees C. At lower pressure (10-15 MPa), a maximum extraction efficiency (63%) was attained for DPT, while the fat is extracted only 28.3%. The increase in temperature is advantageous for the selective extraction of DPT. However, the extraction efficiency of DPT decreased as the extraction temperature was increased. When a lower pressure (10 MPa) and a higher temperature (50 degrees C) were used, the organotin (DPT) was extracted up to a maximum (59.9%) within 30 min, while fat was extracted only 4.9%.
Ding
M
M
Department of Chemistry, Tsinghua University, Beijing, 100084.
Chen
P
P
Luo
G
G
Koizumi
H
H
Suzuki
Y
Y
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D009947:Organotin Compounds / Q000737:chemistry / Q000302:isolation & purification*; D011208:Powders / Q000032:analysis; D013024:Soybean Oil / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
D009947:Organotin Compounds; D011208:Powders; D013024:Soybean Oil