[Immunoaffinity columns and determination of ochratoxin A in cereals by HPLC. Part II. Evaluation of extraction using acetonitrile/water].
Ochratoxin A from wheat and rye grain was extracted with acetonitrile:water (60:40). After clean-up of extracts using immunoaffinity columns (IAC), ochratoxin was determined by high-performance liquid chromatography using C18 column and fluorometric detection at 330 nm excitation and 460 nm emission. The mean recovery of ochratoxin A at fortification levels 0.1-100 microg/kg, was 74-89%. The limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.025 microg/kg, respectively. The positive results were confirmed by reaction with BF3 complex in methanol.
Czerwiecki
Ludwik
L
Zakład Analizy Zywności, Instytut Biotechnologii Przemysłu Rolno-Spozywczego, 02-532 Warszawa, ul. Rakowiecka 36. czerwiecki@ibprs.pl
Czyzyk
Kamila
K
Wilczyńska
Grazyna
G
Kwiecień
Agnieszka
A
D000097:Acetonitriles; D002273:Carcinogens / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D005470:Fluorometry; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D000432:Methanol; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water
D000097:Acetonitriles; D002273:Carcinogens; D009793:Ochratoxins; D014867:Water; C025589:ochratoxin A; D000432:Methanol; C032159:acetonitrile