Use of hand held photopolymerizer to photoinactivate Streptococcus mutans.
OBJECTIVE
The main focus of this research was to investigate the photodynamic therapy (PDT), in vitro, acting on Streptococcus mutans and fibroblasts. A hand held photopolymerizer (HHP) and a classical photosensitizer (Rose Bengal) were used to induce photodynamic response.


METHODS
S. mutans and fibroblast were treated with different concentrations of Rose Bengal (0-50 microM) irradiated with light (400-500 nm) for different time periods (0-40s) and then cell viability was evaluated.


RESULTS
It was observed that the light (per se) is not toxic and in the dark Rose Bengal is toxic to the cells tested only at concentrations above 2.5 microM. Under light exposure concentrations of Rose Bengal above 0.5 microM all S. mutans were killed with no cytotoxic effects to fibroblasts.


CONCLUSIONS
For the purpose of this work, the photoactivation of Rose Bengal, using the HHP, inactivated the bacteria without affecting the fibroblast viability.
Paulino
Tony P
TP
Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, FFCLRP-USP, 14040-901 Ribeirão Preto, SP, Brazil.
Ribeiro
Karina F
KF
Thedei
Geraldo
G
Tedesco
Antônio C
AC
Ciancaglini
Pietro
P
D001431:Bacteriological Techniques; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008027:Light; D010778:Photochemotherapy / Q000295:instrumentation; D017319:Photosensitizing Agents / Q000633:toxicity*; D012395:Rose Bengal / Q000633:toxicity*; D013295:Streptococcus mutans / Q000187:drug effects / Q000528:radiation effects*; D013997:Time Factors
D017319:Photosensitizing Agents; D012395:Rose Bengal