Site-specific labeling of proteins with small molecules in live cells.
The principal bottleneck for the utilization of small-molecule probes in live cells is the shortage of methodologies for targeting them with very high specificity to biological molecules or compartments of interest. Recently developed methods for labeling proteins with small-molecule probes in cells employ special protein or peptide handles that recruit small-molecule ligands, harness enzymes to catalyze small-molecule conjugation or hijack the cell's protein translation machinery.
Chen
Irwin
I
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Ting
Alice Y
AY
D000818:Animals; D002468:Cell Physiological Phenomena; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
D015335:Molecular Probes; D011506:Proteins