title: Crystal structures of alfalfa caffeoyl coenzyme A 3-O-methyltransferase.
abstract: Caffeoyl coenzyme A 3-O-methyltransferases (CCoAOMTs) are S-adenosyl-l-methionine-dependent O-methyltransferases (OMTs) involved in lignin biosynthesis. Plant CCoAOMTs belong to a distinct family of OMTs, more closely related to the mammalian catechol OMTs than to other plant OMTs. The crystal structure of alfalfa (Medicago sativa) CCoAOMT in complex with the reaction products S-adenosine-l-homocysteine and feruloyl/sinapoyl CoAs presented here belong to a structurally and mechanistically distinct family of plant small molecule OMTs. These structures provide a new understanding of the substrate preferences and the catalytic mechanism accompanying CCoAOMT-mediated O-methylation of CoA-linked phenylpropanoid substrates.
authors.affiliation: Laboratoire de Cristallographie et Cristallogenèse des Protéines, Institut de Biologie Structurale, 38027 Grenoble cedex 1, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Ferrer
authors.forename: Chloe
authors.initials: C
authors.lastname: Zubieta
authors.forename: Richard A
authors.initials: RA
authors.lastname: Dixon
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Noel
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D000455:Medicago sativa / Q000201:enzymology*; D008780:Methyltransferases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D008780:Methyltransferases; C059825:caffeoyl-CoA O-methyltransferase
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