title: Kinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2.
abstract: We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Dianah
authors.initials: D
authors.lastname: Barrett
authors.forename: Catherine
authors.initials: C
authors.lastname: Leimkuhler
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Deborah
authors.initials: D
authors.lastname: Walker
authors.forename: Daniel
authors.initials: D
authors.lastname: Kahne
authors.forename: Suzanne
authors.initials: S
authors.lastname: Walker
mesh_terms: D007700:Kinetics; D010457:Peptidoglycan / Q000737:chemistry / Q000378:metabolism; D046928:Peptidoglycan Glycosyltransferase / Q000235:genetics / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics
chemical_list: D010457:Peptidoglycan; D046928:Peptidoglycan Glycosyltransferase
references.citation: J Am Chem Soc. 2001 Nov 28;123(47):11638-43
references.pmid: 11716719
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10886-91
references.pmid: 11517340
references.citation: Biochemistry. 2002 Oct 15;41(41):12552-61
references.pmid: 12369847
references.citation: J Clin Invest. 2003 May;111(9):1265-73
references.pmid: 12727914
references.citation: Proc Natl Acad Sci U S A. 2003 May 13;100(10):5658-63
references.pmid: 12714684
references.citation: J Biol Chem. 2003 May 30;278(22):19898-903
references.pmid: 12663672
references.citation: J Bacteriol. 2003 Aug;185(15):4418-23
references.pmid: 12867450
references.citation: Mol Microbiol. 2004 Jul;53(2):675-85
references.pmid: 15228543
references.citation: Biochemistry. 2004 Sep 28;43(38):12375-81
references.pmid: 15379577
references.citation: Biochem J. 1973 Dec;135(4):721-8
references.pmid: 4778270
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):4200-4
references.pmid: 825860
references.citation: J Biol Chem. 1984 Nov 25;259(22):13937-46
references.pmid: 6389538
references.citation: J Bacteriol. 1992 Jun;174(11):3549-57
references.pmid: 1592809
references.citation: N Engl J Med. 1998 Aug 20;339(8):520-32
references.pmid: 9709046
references.citation: Microbiol Mol Biol Rev. 1998 Dec;62(4):1079-93
references.pmid: 9841666
references.citation: Glycobiology. 2001 Mar;11(3):25R-36R
references.pmid: 11320055
references.citation: J Am Chem Soc. 2001 Apr 4;123(13):3155-6
references.pmid: 11457035
references.citation: J Am Chem Soc. 2002 Apr 10;124(14):3656-60
references.pmid: 11929255