Identification of proteins interacting with Toxoplasma SRCAP by yeast two-hybrid screening.
Toxoplasma gondii is an opportunistic protozoan parasite that differentiates into latent cysts (bradyzoite) that can be reactivated during immunosuppression. TgSRCAP (Toxoplasma gondii Snf2-related CBP activator protein) is a SWI2/SNF2 family chromatin remodeler whose expression increases during cyst development. Identifying the proteins associating with TgSRCAP during the pre-cyst stage (tachyzoite) will increase our understanding of how parasite differentiation is initiated. We employed the yeast two-hybrid system to identify proteins that may interact directly with TgSRCAP. A stretch of 1,060 amino acids between ATPase subdomains IV and V of TgSRCAP was chosen as "bait" since the corresponding region in human SRCAP interacts with other proteins, including CREB binding protein. We have identified several novel parasite-specific transcription factors predicted to be in the T. gondii genome. Metabolic enzymes that may participate in cyst development were also identified as interacting with TgSRCAP.
Nallani
Karuna C
KC
Department of Pharmacology and Toxicology, Indiana University School of Medicine, Medical Sciences Building Room A-525, 635 Barnhill Drive, Indianapolis, IN 46202, USA.
Sullivan
William J
WJ
D000818:Animals; D019295:Computational Biology; D004798:Enzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D010957:Plasmids; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D014122:Toxoplasma / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D004798:Enzymes; D015800:Protozoan Proteins; D014157:Transcription Factors
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