title: Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast.
abstract: Haploinsufficiency is defined as a dominant phenotype in diploid organisms that are heterozygous for a loss-of-function allele. Despite its relevance to human disease, neither the extent of haploinsufficiency nor its precise molecular mechanisms are well understood. We used the complete set of Saccharomyces cerevisiae heterozygous deletion strains to survey the genome for haploinsufficiency via fitness profiling in rich (YPD) and minimal media to identify all genes that confer a haploinsufficient growth defect. This assay revealed that approximately 3% of all approximately 5900 genes tested are haploinsufficient for growth in YPD. This class of genes is functionally enriched for metabolic processes carried out by molecular complexes such as the ribosome. Much of the haploinsufficiency in YPD is alleviated by slowing the growth rate of each strain in minimal media, suggesting that certain gene products are rate limiting for growth only in YPD. Overall, our results suggest that the primary mechanism of haploinsufficiency in yeast is due to insufficient protein production. We discuss the relevance of our findings in yeast to human haploinsufficiency disorders.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, California 94305, USA.
authors.forename: Adam M
authors.initials: AM
authors.lastname: Deutschbauer
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Jaramillo
authors.forename: Michael
authors.initials: M
authors.lastname: Proctor
authors.forename: Jochen
authors.initials: J
authors.lastname: Kumm
authors.forename: Maureen E
authors.initials: ME
authors.lastname: Hillenmeyer
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
authors.forename: Corey
authors.initials: C
authors.lastname: Nislow
authors.forename: Guri
authors.initials: G
authors.lastname: Giaever
mesh_terms: D000483:Alleles; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005799:Genes, Dominant; D005821:Genetic Techniques; D016681:Genome, Fungal; D006579:Heterozygote; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013997:Time Factors
chemical_list: D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins
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