Hemolytic potential of structurally related aniline halogenated hydroxylamines.
This study was undertaken to investigate the hemolytic potential of several structurally related aniline halogenated phenylhydroxylamines based on their decreasing electro negativity. The compounds compared are phenylhydroxylamine (PHA) and para-fluoro-, para-bromo-, and para-iodo-phenylhydroxylamines. Red blood cells of male Sprague-Dawley rats were labeled with radioactive chromium-51 and exposed to the test agent before being infused into the tail vein of isologous rats. The time course of blood radioactivity was monitored. The stability of some selected halogenated aniline analogs was also determined in blood. All four tested hydroxylamines produced dose-dependent reduction in the circulating labeled red blood cells indicating their destruction and loss. The most pronounced reduction was observed at doses from 175 to 250 microM. The dose of 100 microM appeared to be the threshold limit. The para-iodo-PHA was two times more toxic than para-fluoro-PHA in the destruction of red blood cells in rats.
Singh
Harpal
H
Department of Natural Sciences and Mathematics, College of Sciences and Technology, Savannah State University, Savannah, Georgia 31404, USA. Singhh@savstate.edu
Purnell
Elissa T
ET
D000743:Anemia, Hemolytic / Q000139:chemically induced / Q000209:etiology; D000814:Aniline Compounds / Q000097:blood / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D006461:Hemolysis; D006846:Hydrocarbons, Halogenated; D006898:Hydroxylamines / Q000097:blood / Q000633:toxicity*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship
D000814:Aniline Compounds; D006846:Hydrocarbons, Halogenated; D006898:Hydroxylamines