Straightforward detection of the secondary ionisation of the phosphate group and pK determinations by high-resolution solid-state 31P NMR.
The suitability of high-resolution solid-state 31P NMR for a straightforward determination of the protonation state of phosphate groups as well as of their pK2 values extracted from solid state mono : dianionic ratios has been demonstrated.
Gardiennet
Carole
C
UMR CNRS 7565, Universite Henri Poincare, Nancy 1, 54506 Vandoeuvre-les-Nancy, France.
Henry
Bernard
B
Kuad
Paul
P
Spiess
Bernard
B
Tekely
Piotr
P
D000249:Adenosine Monophosphate / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010710:Phosphates / Q000737:chemistry*; D012680:Sensitivity and Specificity
D010710:Phosphates; D000249:Adenosine Monophosphate