Genetically engineered production of 1-desmethylcobyrinic acid, 1-desmethylcobyrinic acid a,c-diamide, and cobyrinic acid a,c-diamide in Escherichia coli implies a role for CbiD in C-1 methylation in the anaerobic pathway to cobalamin.
Co-expression of the cobA gene from Propionibacterium freudenreichii and the cbiA, -C, -D, -E, -T, -F, -G, -H, -J, -K, -L, and -P genes from Salmonella enterica serovar typhimurium in Escherichia coli resulted in the production of cobyrinic acid a,c-diamide. A cbiD deletion mutant of this strain produced 1-desmethylcobyrinic acid a,c-diamide, indicating that CbiD is involved in C-1 methylation in the anaerobic pathway to cobalamin. Strains that did not have the cbiP gene also produced 1-desmethylcobyrinic acid a,c-diamide, and strains that had neither cbiP nor cbiA synthesized 1-desmethylcobyrinic acid even in the presence of cbiD, suggesting that CbiA and CbiP are necessary for CbiD activity.
Roessner
Charles A
CA
Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA. c-roessner@tamu.edu
Williams
Howard J
HJ
Scott
A Ian
AI
D000577:Amides / Q000737:chemistry; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D049109:Cell Proliferation; D003038:Cobamides / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D008745:Methylation; D008956:Models, Chemical; D008958:Models, Molecular; D010957:Plasmids / Q000378:metabolism; D011165:Porphyrinogens / Q000737:chemistry; D011424:Propionibacterium / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism; D013053:Spectrophotometry; D014466:Ultraviolet Rays; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D000577:Amides; D001426:Bacterial Proteins; D003038:Cobamides; D011165:Porphyrinogens; C010895:cobyrinic acid; D014805:Vitamin B 12