Movement behaviour of Medaka (Oryzias latipes) in response to sublethal treatments of diazinon and cholinesterase activity in semi-natural conditions.
Behavioural changes of medaka (Oryzias latipes) treated with an anticholinesterase insecticide, diazinon (0.1 mg L(-1)), were continuously observed for 4 days in semi-natural conditions. Although variations occurred in individual specimens, the movement tracks appeared differently with typical short-range movement with irregular turns and shaking after the treatments. Eight movement patterns frequently observed before and after the treatments were selected, and the variables characterising the movement patterns were compared quantitatively. The variables were clearly differentiated when the movement patterns were correspondingly matched before and after the treatments (e.g., vertical movements, horizontal movements, etc). Meander and stop duration were highly different among the selected movement patterns. Additionally, different degree of toxic response behaviours could also be detected by quantitative characterisation of the variables. Response behaviour was confirmed with toxicological experiments that show the decrease in the acetylcholine esterase activity in the head and body of specimens. Quantitative investigations on the variables of the movement tracks suggested the usefulness of response behaviour as a monitoring tool for environmental assessment.
Chon
Tae-Soo
TS
Division of Biological Sciences, Pusan National University, Pusan, Korea. tschon@pusan.ac.kr
Chung
Namil
N
Kwak
Inn-Sil
IS
Kim
Jong-Sang
JS
Koh
Sung-Cheol
SC
Lee
Sung-Kyu
SK
Leem
Joo-Baek
JB
Cha
Eui Young
EY
D000818:Animals; D002802:Cholinesterases / Q000494:pharmacology*; D003976:Diazinon / Q000633:toxicity*; D004781:Environmental Exposure; D005399:Fishes; D007306:Insecticides / Q000633:toxicity*; D009068:Movement; D013550:Swimming; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007306:Insecticides; D014874:Water Pollutants, Chemical; D002802:Cholinesterases; D003976:Diazinon
Environ Pollut. 2002;120(3):671-81
12442790
Chemosphere. 2003 Jan;50(3):429-36
12656264
J Neurosci Methods. 2000 Mar 15;96(2):119-31
10720676
Bull Environ Contam Toxicol. 1990 Aug;45(2):243-9
2400840
Arch Int Physiol Biochim Biophys. 1992 Sep-Oct;100(5):331-4
1282384
J Toxicol Environ Health. 1994 Dec;43(4):383-418
7990167
J Helminthol. 1992 Jun;66(2):79-88
1379273
Biol Cybern. 1998 Jun;78(6):433-40
9711817
Biol Cybern. 1998 Jun;78(6):423-32
9711816
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1999 Jan;122(1):33-43
10190026
Ecotoxicol Environ Saf. 2000 Nov;47(3):261-5
11139179
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Ecotoxicol Environ Saf. 1986 Apr;11(2):210-8
3709408
J Forensic Sci. 1992 Jan;37(1):337-44
1545210
Biochem Pharmacol. 1961 Jul;7:88-95
13726518