Immunohistochemical study of cyclosporin-induced gingival overgrowth in renal transplant recipients.
BACKGROUND
Cyclosporin A (CsA) is an immunosuppressant widely used to treat transplant patients and various systemic diseases with immunological components. Gingival overgrowth (GO) is a common side effect of CsA administration; however, the pathogenesis of drug-induced GO is poorly understood. The aim of this study was to evaluate the expression of Ki-67, activation molecules (CD71, CD98), leukocytes activation antigens (CD45, CD45RA, CD50, CD11a, CD162, CD227, CD231), neurothelin (CD147), and novel endothelial cell antigens (B-F45, SCF87, B-D46, B-C44, VJ1/6) in gingival tissue in renal transplant recipients treated with CsA.


METHODS
Tissues from 15 renal transplant patients with significant GO and 10 systemically healthy control subjects with gingivitis were studied. Frozen-section biopsies were stained with monoclonal antibodies specific for the above-mentioned antigens using an indirect immunoperoxidase technique.


RESULTS
Comparison of the CsA-treated and control groups revealed no significant differences with respect to expression of Ki-67; CD50; activation molecules; neurothelin; or novel endothelial cell antigens B-D46, B-C44, and VJ1/6. However, expression patterns of CD45, CD45RA, CD11a, CD162, CD227, CD231, B-F45, and SCF87 were significantly different in CsA and control groups.


CONCLUSIONS
Leukocyte activation antigens play an important role in CsA-induced gingival overgrowth.
Bulut
Omer Engin
OE
Hacettepe University, Faculty of Medicine, Department of Histology, Ankara, Turkey.
Sökmensüer
Lale Karakoç
LK
Bulut
Sule
S
Tasman
Fügen
F
Müftüoğlu
Sevda
S
D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D016022:Case-Control Studies; D016572:Cyclosporine / Q000009:adverse effects*; D005260:Female; D005881:Gingiva / Q000276:immunology; D019214:Gingival Overgrowth / Q000139:chemically induced / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D019394:Ki-67 Antigen / Q000096:biosynthesis; D016030:Kidney Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D018709:Statistics, Nonparametric
D015703:Antigens, CD; D000954:Antigens, Surface; D007166:Immunosuppressive Agents; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; C055591:endothelial cell-monocyte antigens; D016572:Cyclosporine