[Toxicity of manganese exposure on the postnatal development of dopaminergic neurons of nigra substance in rat brain].
OBJECTIVE
To study the poisonous effect of manganese on the postnatal development of dopaminergic neurons of nigra substance in rat brain.


METHODS
Morris Water Maze was carried out to examine the muscle motor ability of the rat. Fluorometry was used to determine the dopamine levels of caudate nucleus. Immunochemistry combined with digital imagine analysis was carried out to observe the TH reactivity and its average densities of the TH-positive products in nigra substance and caudate nucleus respectively.


RESULTS
(1) The latency to reach the platform above water in Morris Water Maze was longer and longer with the increasing of Mn-dose exposured. (2) The DA levels of the caudate nucleus, and the TH-immunochemical reactivity as well as the average densities of TH-positive products in nigra substance and caudate was significantly declined respectively with increasing of Mn-dose exposure.


CONCLUSIONS
The postnatal development of dopaminergic neurons in nigra substance of the rat brain was damaged by manganese-exposure, and the positive relationship existed between the damage effects and the Mn-dose exposure.
He
Xinhong
X
Basic College, Guangdong College of Pharmacy, Guangzhou 510224, China.
Zhang
Dexing
D
Chen
Weiqiang
W
Huang
Shaoming
S
D000818:Animals; D000831:Animals, Newborn; D002421:Caudate Nucleus / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020149:Manganese Poisoning / Q000503:physiopathology; D018782:Maze Learning; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027924:Sambucus nigra / Q000378:metabolism*
D004298:Dopamine