Comparative study on schizontocidal activity of recrystallized or crude daphnetin against malaria parasites.
OBJECTIVE
To compare the schizontocidal activity of recrystallized or crude daphnetin against malaria parasites in vivo.


METHODS
Schizontocidal activity of recrystallized or crude daphnetin at various dosages was assessed in mice infected with Plasmodium berghei ANKA using a "4-day suppress assay".


RESULTS
The comparison of the reduction rate of parasitemia caused by either recrystallized or crude dephnetin showed that ED(50) of crude daphnetin was 18.36 mg/kg, with 95% confidence limit of 5.96-56.54 mg/kg while ED50 of recrystallized daphnetin was 11.46 mg/kg, with 95% confidence limit of 8.63-15.22 mg/kg.


CONCLUSIONS
The results indicate that the efficacy of recrystallized daphnetin is 37.6% higher than that of crude daphnetin.
Wang
Qin-Mei
QM
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China. wangqinmei@hotmail.com
Ni
Yi-Chang
YC
Guo
Jian
J
Wu
Jia-Tong
JT
Qian
Ying-Jun
YJ
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology*; D051379:Mice; D021261:Parasitic Sensitivity Tests; D010962:Plasmodium berghei / Q000187:drug effects*; D014468:Umbelliferones / Q000494:pharmacology*
D000962:Antimalarials; D014468:Umbelliferones; C039952:daphnetin