[Separation and detection of seven carbamate pesticides in biological samples by solid phase extraction and high performance liquid chromatography].
Methomyl, metolcarb, propoxur, carbaryl, isoprocarb, diuron and bassa are all carbamate pesticides. In this paper, a reversed-phase HPLC method is described for analysis of these carbamate pesticides in biological samples with Spherisorb C18 column (150 mm x 2.0 mm i.d., 5 microm), 60:40 (V/V) MeOH-H20 eluent and photodiode array detector. These pesticides were identified by their spectrum characteristics and retention times. Their quantity was determined by their peak area. The biological samples were extracted with C18 cartridge and eluted with ethyl acetate, and then analysed by HPLC. The sensitivity of detection for the seven carbamates was in the range of 1-2 mg/L of blood sample and 5-10 mg/L of urine sample. The recoveries of blood and urine samples were 70%-80% and 65%-82%, respectively. The method is simple, rapid and highly sensitive.
Zhang
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Institute of pharmacology and Toxicology, Academy of Military Sciences, Beijing, 100850.
D002219:Carbamates / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
D002219:Carbamates; D010573:Pesticide Residues