title: The vertebral interbody grafting site's low concentration in osteogenic progenitors can greatly benefit from addition of iliac crest bone marrow.
abstract: The ability of bone substitutes to promote bone fusion is contingent upon the presence of osteoinductive factors in the bone environment at the fusion site. Osteoblast progenitor cells are among these environmental osteoinductive factors, and one of the most abundant and available sources of osteoblastic cells is the bone marrow. As far as biological conditions are concerned, the vertebral interbody space appears as a favorable site for fusion, as it is surrounded by spongy bone, theoretically rich in osteogenic cells. This site may, however, not be as rich in osteogenic precursor cells especially at the time of grafting, because decortication of the vertebral end plates during the grafting process may modify cell content of the surrounding spongy bone. We tested this hypothesis by comparing the abundance of human osteogenic precursor cells in bone marrow derived from the iliac crest, the vertebral body, and the decorticated intervertebral body space. The number of potential osteoblast progenitors in each site was estimated by counting the alkaline phosphatase-expressing colony-forming units (CFU-AP). The results, however, demonstrate that the vertebral interbody space is actually poorer in osteoprogenitor cells than the iliac crest (P<0.001) and vertebral body (P<0.01), especially at the time of graft implantation. In light of our results, we advocate addition of iliac crest bone marrow aspirate to increase the success rate of vertebral interbody fusion.
authors.affiliation: Department of Orthopaedic Surgery, Hôtel Dieu, University Hospital of Nantes, 44093 Nantes, France.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Romih
authors.forename: Joël
authors.initials: J
authors.lastname: Delécrin
authors.forename: Dominique
authors.initials: D
authors.lastname: Heymann
authors.forename: Norbert
authors.initials: N
authors.lastname: Passuti
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D016025:Bone Transplantation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007085:Ilium / Q000637:transplantation; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000166:cytology* / Q000601:surgery*; D008297:Male; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D013123:Spinal Fusion / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D000469:Alkaline Phosphatase
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