Evaluation of home allergen sampling devices.
BACKGROUND
Simple, inexpensive methods of sampling from allergen reservoirs are necessary for large-scale studies or low-cost householder-operated allergen measurement.


METHODS
We tested two commercial devices: the Indoor Biotechnologies Mitest Dust Collector and the Drager Bio-Check Allergen Control; two devices of our own design: the Electrostatic Cloth Sampler (ECS) and the Press Tape Sampler (PTS); and a Vacuum Sampler as used in many allergen studies (our Reference Method). Devices were used to collect dust mite allergen samples from 16 domestic carpets. Results were examined for correlations between the sampling methods.


RESULTS
With mite allergen concentration expressed as microg/g, the Mitest, the ECS and the PTS correlated with the Reference Method but not with each other. When mite allergen concentration was expressed as microg/m2 the Mitest and the ECS correlated with the Reference Method but the PTS did not. In the high allergen conditions of this study, the Drager Bio-Check did not relate to any methods.


CONCLUSIONS
The Mitest Dust Collector, the ECS and the PTS show performance consistent with the Reference Method. Many techniques can be used to collect dust mite allergen samples. More investigation is needed to prove any method as superior for estimating allergen exposure.
Sercombe
J K
JK
Woolcock Institute of Medical Research, The University of Sydney, NSW, Australia.
Liu-Brennan
D
D
Garcia
M L
ML
Tovey
E R
ER
D000485:Allergens / Q000032:analysis*; D000818:Animals; D004391:Dust / Q000276:immunology; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000592:standards; D004867:Equipment Design; D005430:Floors and Floorcoverings; D006798:Housing; D006801:Humans; D008925:Mites / Q000276:immunology
D000485:Allergens; D004391:Dust