[Estimation of detection limit from slope of B/B0 in competitive ELISA].
A method has been proposed for determining the detection limit (L(D)) from the slope of a semilogarithmic plot of a B/B(0) curve in competitive enzyme linked immunosorbent assay (ELISA). As an application, this paper describes a graphic determination of L(D) from analogue data in the literature. The L(D) obtained corresponds to the concentration at which the relative standard deviation of concentration estimates is 30%.
Hayashi
Yuzuru
Y
National Institute of Health Sciences, Tokyo 158-8501, Japan. fumi@nihs.go.jp
Matsuda
Rieko
R
Ito
Katsutoshi
K
Imai
Kazuhiro
K
Maeda
Masako
M
D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D016015:Logistic Models; D012680:Sensitivity and Specificity