Cerebrospinal fluid soluble CD27 levels in children with non-Hodgkin lymphomas.
The authors investigated the diagnostic value of cerebrospinal fluid (CSF) soluble CD27 (sCD27) for leptomeningeal involvement of non-Hodgkin lymphomas (NHL). Cytospin slides were prepared from CSF samples of 64 children treated for NHL. sCD27 levels were determined by sandwich ELISA method using two CD27 monoclonal antibodies. 8/194 (4.1%) samples were considered tumor-positive by cytology. Mean sCD27 values were 5.8 and 13.8 U/mL in tumor-negative and tumor-positive samples, respectively (p =.18). 26/194 samples were false positive and 2/194 false negative (cutoff: 7 U/mL) (sensitivity, 75 %; specificity, 86%; positive predictive value, 18.8%; negative predictive value, 98.8%; accuracy, 85.6%). With these results, the value of adding sCD27 determination to the cytological CSF examination remains questionable.
Yalçin
Bilgehan
B
Hacettepe University Institute of Oncology, Department of Pediatric Oncology, Ankara, Turkey. yalcinb@hacettepe.edu.tr
Canpinar
Hande
H
Kutluk
M Tezer
MT
Varan
Ali
A
Akyüz
Canan
C
Büyükpamukçu
Münevver
M
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003951:Diagnostic Errors; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008228:Lymphoma, Non-Hodgkin / Q000134:cerebrospinal fluid* / Q000473:pathology; D008297:Male; D008577:Meningeal Neoplasms / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D016008:Statistical Distributions; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000134:cerebrospinal fluid*
D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7