Slow release chelate enhancement of lead phytoextraction by corn (Zea mays L.) from contaminated soil--a preliminary study.
Short-term enhancement of lead (Pb) and zinc (Zn) uptake by corn (Zea mays L.) seedlings from a contaminated soil was compared using slow-release coated EDTA granules-a coated chelating agent (CCA), uncoated EDTA granules, and EDTA solution in a greenhouse experiment. Soil Pb and Zn fractions were determined using a sequential extraction scheme. Release of the metals in the soil was examined in a column leaching study. After only 7 days of seedling growth, shoot biomass was decreased by all EDTA treatments compared with the zero-EDTA control. The amount of shoot biomass produced was highest with uncoated EDTA, intermediate with CCA, and lowest with the EDTA solution. Shoot Pb contents were highest with solid EDTA, intermediate with CCA, and lowest with EDTA solution, and they were always higher with EDTA treatments than in controls. In contrast, shoot Zn contents following EDTA treatments were lower than in the control. Levels of soil dissolved organic carbon (DOC) in aqueous soil extracts were much lower after CCA application than following treatments with solid EDTA and EDTA solution. After 17 days of plant growth, when most of the chelating agent had been released from the CCA, soil organic carbon levels remained relatively constant and similar to those in the control, indicating that a relatively low chelating agent concentration can be maintained for the plants to take up the metals. The distribution of Pb in the sequential extraction procedure showed that the Pb level in the exchangeable+carbonate-bound fraction with CCA was significantly lower than that with solid EDTA or EDTA solution, further indicating that slow release of CCA improves the bioavailability of metals in the soil to match plant uptake of those metals. The results suggest that CCA can enhance shoot content of Pb but not of Zn from the contaminated soil in the short term, and may also reduce the risk of metal leaching from the soil.
Li
Haifeng
H
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Wang
Qingren
Q
Cui
Yanshan
Y
Dong
Yiting
Y
Christie
Peter
P
D018533:Biomass; D002244:Carbon / Q000032:analysis / Q000378:metabolism; D002254:Carbonates / Q000032:analysis / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004787:Environmental Pollution; D007854:Lead / Q000378:metabolism*; D009930:Organic Chemicals / Q000032:analysis / Q000378:metabolism; D018570:Risk Assessment; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D012989:Soil Pollutants / Q000378:metabolism*; D013997:Time Factors; D003313:Zea mays / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
D002254:Carbonates; D002614:Chelating Agents; D009930:Organic Chemicals; D012989:Soil Pollutants; D007854:Lead; D002244:Carbon; D004492:Edetic Acid; D015032:Zinc