title: Antecedent ethanol ingestion prevents postischemic P-selectin expression in murine small intestine.
abstract: OBJECTIVE
  Ethanol ingestion 24 h prior to ischemia and reperfusion (I/R) prevents postischemic leukocyte rolling and adhesion in postcapillary venules of the small bowel. Since I/R-induced leukocyte rolling is critically dependent on the expression of P-selectin by endothelial cells lining postcapillary venules, the authors hypothesized that antecedent ethanol consumption would attenuate postischemic expression of this adhesive ligand.
  
  
  METHODS
  To address this postulate, P-selectin expression was evaluated using a dual radiolabeled monoclonal antibody technique in the jejunum of mice that received either distilled water vehicle or ethanol by gavage (dose) on day 1 and then were subjected to sham I/R (nonischemic controls) or I/R (20 min ischemia/60 min reperfusion) 24 h later.
  
  
  RESULTS
  I/R was associated with a 2-fold increase in P-selectin expression relative to nonischemic controls, an effect that was largely abolished by antecedent ethanol ingestion. Exposing the bowel to adenosine deaminase or adenosine A2 receptor antagonists (DMPX or ZM241385), an NO synthase inhibitor (L-NIO) or an NO scavenger (PTIO), or an antioxidant (mercaptoproprionyl glycine) during the period of ethanol exposure on day 1 prevented the beneficial effect of ethanol to limit I/R-induced P-selectin expression, on day 2.
  
  
  CONCLUSIONS
  The data indicate that antecedent ethanol exposure prevents postischemic P-selectin expression on day 2 by a mechanism that is triggered by adenosine A2 receptor activation and the formation of nitric oxide (NO) and reactive oxygen species (ROS) during the period of ethanol exposure on day 1.
authors.affiliation: Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, School of Medicine in Shreveport, Shreveport, Louisiana, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Dayton
authors.forename: Taiji
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kamada
authors.forename: Patsy
authors.initials: P
authors.lastname: Carter
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Korthuis
mesh_terms: D000818:Animals; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism; D019007:P-Selectin / Q000187:drug effects* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D043683:Receptors, Adenosine A2 / Q000378:metabolism; D015427:Reperfusion Injury
chemical_list: D019007:P-Selectin; D017382:Reactive Oxygen Species; D043683:Receptors, Adenosine A2; D009569:Nitric Oxide; D000431:Ethanol