Transcription, translation, and immunolocalization of ODVP-6E/ODV-E56 and p74 proteins: two highly conserved ODV-associated envelope proteins of Choristoneura fumiferana Granulovirus.
Choristoneura fumiferana granulovirus (ChfuGV) infection results two types of enveloped virions: Occlusion-derived virus (ODV) and budded virus (BV). Structural proteins ODVP-6E/ODV-E56 and p74 are two major conserved ODV-associated proteins that may be involved in the initiation of viral infection cycle in susceptible host insect larvae. This study presents the characterization of ChfuGV odvp-6e/odv-e56 and p74 transcription and translation as well as immunolocalization of these proteins in the occluded ChfuGV virion. Our results revealed that the transcription of odvp-6e/odv-e56 and p74 genes, both, start at 24 hours post infection (h p.i.). Using monospecific polyclonal antibodies made against ODVP-6E/ODV-E56 and p74 we demonstrated that these proteins are both expressed late in infection (24 h p.i.). Immunogold labeling using antisera against ODVP-6E/ODV-E56 and p74 proteins demonstrated that ODVP-6E/ODV-E56 and p74 proteins are both associated with the ODV envelop of ChfuGV.
Rashidan
Kianoush Khajeh
KK
Institut national de la recherche scientifique-Institut Armand-Frappier 531 Blvd des Prairies, H7V 1B7, Laval, Canada. kiarashidan@juno.com
Nassoury
Nasha
N
Giannopoulos
Paresa N
PN
Guertin
Claude
C
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D017124:Conserved Sequence; D025722:Granulovirus / Q000378:metabolism*; D007181:Inclusion Bodies, Viral; D007814:Larva / Q000821:virology; D007915:Lepidoptera / Q000254:growth & development / Q000821:virology*; D016253:Microscopy, Immunoelectron; D009036:Moths / Q000821:virology*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
C098821:ODVP-6E protein, baculovirus; D014759:Viral Envelope Proteins