Oxidative activity of human polymorphonuclear leukocytes stimulated by the long-chain phosphatidic acids.
It has already been suggested that phosphatidic acids (PAs) play an important role in the regulation of signaling pathways involved in the production of reactive oxygen species (ROS) by human polymorphonuclear leukocytes (PMNs). The present study was performed to elucidate the effects of extracellularly added PA-- 1,2-distearoyl- (DSPA) and 1-stearoyl-2-arachidonoyl-sn-glycero-phosphate (SAPA)--on the ROS production and on the elastase release by human PMNs. ROS production was monitored by luminol-amplified chemiluminescence and the elastase activity was measured in the supernatant of the PA-stimulated human PMNs by colorimetric assay. Obtained effects were compared with those of cells stimulated by either a chemotactic tripeptide, phorbol ester or calcium ionophore. Our results show that long-chain PAs at concentrations higher than 3 x 10(-5) mol/l stimulate the ROS production by human PMNs, whereas they were ineffective in promoting the elastase release. The chemiluminescence pattern of the SAPA-stimulated cells exhibited a biphasic curve, whereas cell stimulation with DSPA resulted in a monophasic chemiluminescence curve. Stimulation of the ROS production by PAs in dependence of the fatty acid composition required the activity of protein kinases.
Petković
M
M
Institute of Medical Physics and Biophysics, Medical Faculty University of Leipzig, Härtelstrasse 16/18, D-04107 Leipzig, Germany. petm@medizin.uni-leipzig
Vocks
A
A
Schiller
J
J
Arnhold
J
J
D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008055:Lipids / Q000032:analysis; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010196:Pancreatic Elastase / Q000378:metabolism; D010712:Phosphatidic Acids / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007202:Indicators and Reagents; D008055:Lipids; D010712:Phosphatidic Acids; D008165:Luminol; D010196:Pancreatic Elastase