title: Goat endothelial cells may be infected in vitro by transmigration of caprine arthritis-encephalitis virus-infected leucocytes.
abstract: The caprine arthritis-encephalitis lentivirus (CAEV) causes a lifelong persistent infection in goats, and induces infiltrations of leucocytes and tissue reorganization in target organs, with a cyclical pattern of viral expression. The mammary gland is an important site of infection, associated with mother-to-kid transmission by infected cells in colostrum and milk. The monocyte/macrophage is the principal target cell, but other cell types, including epithelial and endothelial cells and fibroblasts, are susceptible to in vitro infection with varying levels of viral replication. Such cells, perhaps at specific differentiation states, might play a role in the regulation and transfer of in vivo infection in target organs. In this paper we describe the in vitro infection of endothelial cell monolayers by the transmigration of monocytes carrying the CAEV provirus. The infected endothelial cells progress to expression of the viral p30 capsid antigen, suggesting viral proliferation. Such a process occurring in vivo during angiogenesis and leucocyte homing to the mammary gland in the final third of mammogenesis, might contribute to viral spread in this crucial target organ.
authors.affiliation: UMR 754, Rétrovirus et Pathologie comparée, INRA/UCBL/ENVL/EPHE, IFR 128 Biosciences Lyon Gerland, Université Claude Bernard, 50 Avenue Tony Garnier, 69366 Lyon Cedex 07, France.
authors.forename: E
authors.initials: E
authors.lastname: Lechat
authors.forename: N
authors.initials: N
authors.lastname: Milhau
authors.forename: P
authors.initials: P
authors.lastname: Brun
authors.forename: C
authors.initials: C
authors.lastname: Bellaton
authors.forename: T
authors.initials: T
authors.lastname: Greenland
authors.forename: J F
authors.initials: JF
authors.lastname: Mornex
authors.forename: C
authors.initials: C
authors.lastname: Le Jan
mesh_terms: D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D042783:Endothelial Cells / Q000276:immunology / Q000821:virology*; D005260:Female; D015511:Goat Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology*; D006041:Goats; D006651:Histocytochemistry / Q000662:veterinary; D016180:Lentivirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D008321:Mammary Glands, Animal / Q000276:immunology / Q000821:virology; D009000:Monocytes / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004279:DNA, Viral