title: Concurrent translocation of BCL2 and MYC with a single immunoglobulin locus in high-grade B-cell lymphomas.
abstract: B-cell leukaemia or lymphoma with a combination of t(8;14)(q24;q32) of Burkitt leukaemia/lymphoma and t(14;18)(q32;q21) of follicular lymphoma may present clinically as de novo acute lymphoblastic leukaemia or transformation of follicular lymphoma to aggressive histology diffuse lymphoma. A number of cell lines have been reported with a complex t(8;14;18) with fusion of MYC, IGH and BCL2 on the same derivative 8 chromosome. The objective of this study was to determine the frequency and chromosomal features of this der(8)t(8;14;18) in a series of acute leukaemias and malignant lymphomas. A database of 1350 leukaemia and lymphoma karyotypes was searched for cases with structural alterations affecting both 8q24 and 18q21. A total of 55 cases were identified, of which eight revealed a complex der(8)t(8;14;18) with an MYC-IGH-BCL2 rearrangement resulting from translocation of BCL2 and MYC with a single disrupted IGH allele. Molecular cytogenetic investigation is essential to identify cases of high-grade leukaemia/lymphoma with concurrent translocations affecting the BCL2 and MYC loci.
authors.affiliation: Department of Pathology and Laboratory Medicine, British Columbia Cancer Agency, University of British Columbia, Vancouver, BC, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Knezevich
authors.forename: O
authors.initials: O
authors.lastname: Ludkovski
authors.forename: C
authors.initials: C
authors.lastname: Salski
authors.forename: V
authors.initials: V
authors.lastname: Lestou
authors.forename: M
authors.initials: M
authors.lastname: Chhanabhai
authors.forename: W
authors.initials: W
authors.lastname: Lam
authors.forename: R
authors.initials: R
authors.lastname: Klasa
authors.forename: J M
authors.initials: JM
authors.lastname: Connors
authors.forename: M J S
authors.initials: MJ
authors.lastname: Dyer
authors.forename: R D
authors.initials: RD
authors.lastname: Gascoyne
authors.forename: D E
authors.initials: DE
authors.lastname: Horsman
mesh_terms: D000328:Adult; D000368:Aged; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D002898:Chromosomes, Human, Pair 8; D005260:Female; D019254:Genes, bcl-2 / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008224:Lymphoma, Follicular / Q000235:genetics; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains