title: Differential regulation of FLOWERING LOCUS C expression by vernalization in cabbage and Arabidopsis.
abstract: Vernalization is required to induce flowering in cabbage (Brassica oleracea var Capitata L.). Since FLOWERING LOCUS C (FLC) was identified as a major repressor of flowering in the vernalization pathway in Arabidopsis (Arabidopsis thaliana), two homologs of AtFLC, BoFLC3-2 and BoFLC4-1, were isolated from cabbage to investigate the molecular mechanism of vernalization in cabbage flowering. In addition to the sequence homology, the genomic organization of cabbage FLC is similar to that of AtFLC, except that BoFLC has a relatively smaller intron 1 compared to that of AtFLC. A vernalization-mediated decrease in FLC transcript level was correlated with an increase in FT transcript level in the apex of cabbage. This observation is in agreement with the down-regulation of FT by FLC in Arabidopsis. Yet, unlike that in Arabidopsis, the accumulation of cabbage FLC transcript decreased after cold treatment of leafy plants but not imbibed seeds, which is consistent with the promotion of cabbage flowering by vernalizing adult plants rather than seeds. To further dissect the different regulation of FLC expression between seed-vernalization-responsive species (e.g. Arabidopsis) and plant-vernalization-responsive species (e.g. cabbage), the pBoFLC4-1BoFLC4-1GUS construct was introduced into Arabidopsis to examine its vernalization response. Down-regulation of the BoFLC4-1GUS construct by seed vernalization was unstable and incomplete; in addition, the expression of BoFLC4-1GUS was not suppressed by vernalization of transgenic rosette-stage Arabidopsis plants. We propose a hypothesis to illustrate the distinct mechanism by which vernalization regulates the expression of FLC in cabbage and Arabidopsis.
authors.affiliation: Institute of BioAgricultural Sciences, Academia Sinica, Taipei 115, Taiwan R.O.C.
authors.forename: Shu-I
authors.initials: SI
authors.lastname: Lin
authors.forename: Jhy-Gong
authors.initials: JG
authors.lastname: Wang
authors.forename: Suk-Yean
authors.initials: SY
authors.lastname: Poon
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Su
authors.forename: Shyh-Shyan
authors.initials: SS
authors.lastname: Wang
authors.forename: Tzyy-Jen
authors.initials: TJ
authors.lastname: Chiou
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis*; D001937:Brassica / Q000254:growth & development / Q000378:metabolism*; D002874:Chromosome Mapping; D003080:Cold Temperature; D035264:Flowers / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D007438:Introns; D026161:MADS Domain Proteins / Q000096:biosynthesis*; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C418902:At5g10140 protein, Arabidopsis; D026161:MADS Domain Proteins; D010940:Plant Proteins
references.citation: Science. 2000 Oct 13;290(5490):344-7
references.pmid: 11030654
references.citation: Mol Gen Genet. 1993 Feb;237(1-2):171-6
references.pmid: 8455554
references.citation: Plant Cell. 1998 Dec;10(12):1973-90
references.pmid: 9836739
references.citation: Plant Cell. 1999 Mar;11(3):445-58
references.pmid: 10072403
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:345-370
references.pmid: 15012238
references.citation: EMBO J. 2002 Aug 15;21(16):4327-37
references.pmid: 12169635
references.citation: Development. 2002 Dec;129(23):5349-61
references.pmid: 12403707
references.citation: Plant Cell. 2003 Nov;15(11):2603-11
references.pmid: 14555696
references.citation: Biol Chem. 1997 Oct;378(10):1079-101
references.pmid: 9372178
references.citation: Mol Gen Genet. 1994 Jan;242(1):81-9
references.pmid: 7904045
references.citation: Plant Physiol. 1964 Sep;39(5):832-5
references.pmid: 16656010
references.citation: Am J Bot. 1999 Apr;86(4):470-5
references.pmid: 10205066
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant Cell. 2003 Jul;15(7):1671-82
references.pmid: 12837955
references.citation: Plant J. 2001 Dec;28(5):545-53
references.pmid: 11849594
references.citation: Plant Cell. 2002;14 Suppl:S111-30
references.pmid: 12045273
references.citation: Plant Cell. 1996 May;8(5):831-45
references.pmid: 8672883
references.citation: EMBO J. 1992 Jan;11(1):251-63
references.pmid: 1346760
references.citation: Annu Rev Genet. 2003;37:371-92
references.pmid: 14616066
references.citation: Genetics. 2003 May;164(1):347-58
references.pmid: 12750345
references.citation: Genes Dev. 2000 Sep 15;14(18):2366-76
references.pmid: 10995392
references.citation: Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):287-91
references.pmid: 11607346
references.citation: Curr Opin Plant Biol. 2004 Oct;7(5):570-4
references.pmid: 15337100
references.citation: Annu Rev Cell Dev Biol. 1999;15:519-50
references.pmid: 10611971
references.citation: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3753-8
references.pmid: 10716723
references.citation: Genes Dev. 2001 Apr 1;15(7):912-24
references.pmid: 11297514
references.citation: Plant J. 2002 Sep;31(5):663-73
references.pmid: 12207655
references.citation: Plant Cell. 2000 Jun;12(6):885-900
references.pmid: 10852935
references.citation: Genetics. 2002 Nov;162(3):1457-68
references.pmid: 12454088
references.citation: Curr Opin Plant Biol. 2000 Feb;3(1):37-42
references.pmid: 10679453
references.citation: EMBO J. 1987 Dec 20;6(13):3901-7
references.pmid: 3327686
references.citation: Nature. 2004 Jan 8;427(6970):159-64
references.pmid: 14712276
references.citation: Nature. 1995 Aug 10;376(6540):490-8
references.pmid: 7637780
references.citation: Plant Cell. 1999 May;11(5):949-56
references.pmid: 10330478
references.citation: Science. 2000 Jun 2;288(5471):1613-6
references.pmid: 10834834
references.citation: Development. 2004 Aug;131(15):3615-26
references.pmid: 15229176
references.citation: FEMS Microbiol Lett. 1999 May 15;174(2):247-50
references.pmid: 10339815
references.citation: Genetics. 1997 Jul;146(3):1123-9
references.pmid: 9215913
references.citation: Plant Cell. 2002 Oct;14(10):2527-37
references.pmid: 12368502
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3281-5
references.pmid: 14973192
references.citation: Science. 2002 Apr 12;296(5566):285-9
references.pmid: 11951029
references.citation: Science. 2003 Dec 5;302(5651):1751-4
references.pmid: 14593187
references.citation: Proc Natl Acad Sci U S A. 1996 May 14;93(10):4793-8
references.pmid: 8643482
references.citation: Genetics. 2003 Apr;163(4):1457-65
references.pmid: 12702689
references.citation: Nature. 2004 Jan 8;427(6970):164-7
references.pmid: 14712277
references.citation: Plant Cell. 2001 Apr;13(4):935-41
references.pmid: 11283346
references.citation: Development. 2004 Aug;131(16):3829-38
references.pmid: 15289433
references.citation: Plant Cell Physiol. 2001 Oct;42(10):1156-68
references.pmid: 11673632