title: DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus.
abstract: Flow cytometry and epifluorescence microscopy results for the euryarchaeon Methanothermobacter thermautotrophicus were consistent with filaments containing multiple cells. Filaments of one to four cells contained two to eight nucleoids. Single chromosome-containing cells were not observed. Filaments containing multiple genome copies displayed synchronous DNA replication initiation. Chromosome segregation occurred during replication or rapidly after replication termination.
authors.affiliation: Department of Biology (Area 5), University of York, PO Box 373, York YO10 5YW, United Kingdom.
authors.forename: Alan I
authors.initials: AI
authors.lastname: Majerník
authors.forename: Magnus
authors.initials: M
authors.lastname: Lundgren
authors.forename: Paul
authors.initials: P
authors.lastname: McDermott
authors.forename: Rolf
authors.initials: R
authors.lastname: Bernander
authors.forename: James P J
authors.initials: JP
authors.lastname: Chong
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D019847:Chromosomes, Archaeal / Q000502:physiology*; D004261:DNA Replication / Q000502:physiology*; D019641:DNA, Archaeal / Q000502:physiology*; D017013:Methanobacteriaceae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D019641:DNA, Archaeal
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