[Effects of p,p'-DDE on intracellular MAPK pathway of rat Sertoli cells in vitro].
OBJECTIVE
To study effects of p,p'-DDE on intracellular p-ERK of rat Sertoli cells in vitro.


METHODS
We cultured Sertoli cells separated from testicular tissue of rats and examined the changes of intracellular p-ERK, using immunohistochemical SABC method.


RESULTS
It was showed that intracellular p-ERK increased with the dose of p,p'-DDE and had a dose-dependent trend.


CONCLUSIONS
The results suggested that p,p'-DDE could result in the phosphorylation of ERK and induce the activation of the downstream road and the transcription and expression of genes.
Wang
Chong
C
Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liang
Jiaojun
J
Wang
Aiguo
A
Yang
Kedi
K
D000818:Animals; D002478:Cells, Cultured; D003633:Dichlorodiphenyl Dichloroethylene / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*
D003633:Dichlorodiphenyl Dichloroethylene; D048049:Extracellular Signal-Regulated MAP Kinases