Human adipose stromal cells expanded in human serum promote engraftment of human peripheral blood hematopoietic stem cells in NOD/SCID mice.
Human mesenchymal stem cells (hMSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles, and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle, and neuron. Therefore, hMSC are attractive candidates for cell and gene therapy. The optimal conditions for hMSC expansion require medium supplemented with fetal bovine serum (FBS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FBS proteins. In this study, we cultured human adipose stromal cells (hADSC) and bone marrow stroma cells (HBMSC) in human serum (HS) during their isolation and expansion, and demonstrated that they maintain their proliferative capacity and ability for multilineage differentiation and promote engraftment of peripheral blood-derived CD34(+) cells mobilized from bone marrow in NOD/SCID mice. Our results indicate that hADSC and hBMSC cultured in HS can be used for clinical trials of cell and gene therapies, including promotion of engraftment after allogeneic HSC transplantation.
Kim
Su Jin
SJ
Department of Physiology, College of Medicine, Pusan National University, Pusan 602-739, Republic of Korea. kara8481@hanmail.net
Cho
Hyun Hwa
HH
Kim
Yeon Jeong
YJ
Seo
Su Yeong
SY
Kim
Han Na
HN
Lee
Jae Bong
JB
Kim
Jae Ho
JH
Chung
Joo Seop
JS
Jung
Jin Sup
JS
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D049109:Cell Proliferation; D017690:Cell Transplantation; D015316:Genetic Therapy; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000166:cytology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010006:Osteoblasts / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology*; D013997:Time Factors
D018952:Antigens, CD34; D017493:Leukocyte Common Antigens