[Studies on anti-hyperglycemic effect and its mechanism of Dendrobium candidum].
OBJECTIVE
To study the anti-hyperglycemic effect and its mechanism of Dendrobium candidum (DC).


METHODS
Normal mice, adrenaline-induced hyperglycemic mice, streptozotocin-induced diabetic (STZ-DM) rats were used. The mechanisms of the anti-hyperglycemic action were studied with radio-immunoassay, immunohistochemical HRP-SPA stain, etc.


RESULTS
DC could not obviously decrease the serum glucose concentrations and insulin levels in normal mice. It could increase serum insulin levels and decrease serum glucagons concentrations in STZ-DM rats. The results of immunohistochemical stain demonstrated that the number of islet beta cells was increased and that of islet a cells was decreased in STZ-DM rats. It could also decrease the serum glucose concentrations and increase liver glucogen contents in adrenaline-induced hyperglycemic mice.


CONCLUSIONS
DC has obvious anti-hyperglycemic effects in adrenaline-induced hyperglycemic mice and STZ-DM rats. Its mechanisms are stimulating the secretion of insulin from beta cells and inhibiting the secretion of glucagons from a cells, and it can probably decrease the decomposition of liver glucogen and increase the synthesis of liver glucogen.
Wu
Hao-shu
HS
Department of Pharmacology and Toxicology, Collage of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China. haoshu-wu@yahoo.com.cn
Xu
Jian-hua
JH
Chen
Li-zuan
LZ
Sun
Ji-jun
JJ
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D031667:Dendrobium / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004837:Epinephrine; D005260:Female; D005934:Glucagon / Q000097:blood; D006003:Glycogen / Q000378:metabolism; D007003:Hypoglycemia / Q000139:chemically induced / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D007328:Insulin; D006003:Glycogen; D005934:Glucagon; D004837:Epinephrine