A novel mycological analysis valuable for evaluating therapeutic efficacy of antimycotics against experimental dermatophytosis in guinea pigs.
Although antimycotic effects are mainly evaluated with regard to whether or not the fungi grow from a specimen obtained from the drug-treated skin, the potential for discrepancies in skin specimens in which the fungi are grown has not been evaluated, in the experimental tinea model. In this study, to evaluate the therapeutic effectiveness of antimycotic agents against fungal skin infection, a novel form of mycological assessment, which focuses on the size of colonies grown from skin specimens was examined and developed. When microconidia of Trichophyton mentagrophytes were inoculated onto a Sabouraud dextrose agar (SDA) plate and incubated at 27 degrees C for 5 days, a linear relationship was observed between the growth area of mycelia and the logarithm of the quantity of microconidia. This relationship between the growth area and the logarithm of the number of T. mentagrophytes microconidia did not change with the addition of skin homogenate and/or keratin powder. Next, the number of fungi in skin blocks attendant upon experimental, cutaneous infection in guinea pigs was evaluated and analyzed via a calibration curve, determined based on a microconidium suspension of T. mentagrophytes. Estimates of severity of dermatophytic infection in experimental animals were parallel to, but more reliable than, results obtained via the conventional mycological method (fungus-positive skin ratio of treated skin) in culture studies of infected dermal tissues. This new analytical method may also be applicable to the in vivo assessment of the therapeutic effect against dermatophytosis experimentally produced in guinea pigs.
Majima
Toshiro
T
Pharmaceutical R&D Laboratories, POLA Chemical Industries, Inc., Yokohama, Kanagawa, Japan. t-majima@pola.co.jp
Masui
Shigeki
S
Uchida
Katsuhisa
K
Yamaguchi
Hideyo
H
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D025301:Hyphae / Q000254:growth & development; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012867:Skin / Q000382:microbiology*; D014008:Tinea Pedis / Q000188:drug therapy* / Q000382:microbiology; D014249:Trichophyton / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification
D000935:Antifungal Agents