title: Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems.
abstract: The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococcus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture performed in a flask (batch condition) using the same culture medium showed that only members of the genus Thermococcus were cultivated. Therefore, continuous enrichment culture using the gas-lift bioreactor can be considered as an efficient and improved method for investigating microbial communities originating from deep-sea hydrothermal vents.
authors.affiliation: UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France. Anne.Postec@ifremer.fr
authors.forename: Anne
authors.initials: A
authors.lastname: Postec
authors.forename: Laurent
authors.initials: L
authors.lastname: Urios
authors.forename: Françoise
authors.initials: F
authors.lastname: Lesongeur
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.forename: Joël
authors.initials: J
authors.lastname: Querellou
authors.forename: Anne
authors.initials: A
authors.lastname: Godfroy
mesh_terms: D001267:Atlantic Ocean; D001407:Bacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001483:Base Sequence; D019149:Bioreactors; D003470:Culture Media; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D020565:Epsilonproteobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019711:Pyrococcus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D019712:Thermococcus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
references.citation: Int J Syst Evol Microbiol. 2002 Jul;52(Pt 4):1331-9
references.pmid: 12148648
references.citation: FEMS Microbiol Ecol. 2001 Mar;35(1):19-26
references.pmid: 11248386
references.citation: Int J Syst Bacteriol. 1999 Apr;49 Pt 2:351-9
references.pmid: 10319455
references.citation: FEMS Microbiol Ecol. 2001 Jan;34(3):213-220
references.pmid: 11137601
references.citation: Environ Microbiol. 2000 Oct;2(5):506-15
references.pmid: 11233159
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: FEMS Microbiol Ecol. 2002 Aug 1;41(2):105-14
references.pmid: 19709244
references.citation: Antonie Van Leeuwenhoek. 2000 Feb;77(2):117-33
references.pmid: 10768471
references.citation: Evolution. 1985 Jul;39(4):783-791
references.pmid: 28561359
references.citation: Int J Syst Evol Microbiol. 2002 Jul;52(Pt 4):1317-23
references.pmid: 12148646
references.citation: Antonie Van Leeuwenhoek. 1998 Jan;73(1):127-41
references.pmid: 9602286
references.citation: Appl Environ Microbiol. 2000 Sep;66(9):3798-806
references.pmid: 10966393
references.citation: Microbiol Rev. 1995 Mar;59(1):143-69
references.pmid: 7535888
references.citation: FEMS Microbiol Lett. 2000 May 1;186(1):127-32
references.pmid: 10779724
references.citation: Int J Syst Evol Microbiol. 2002 Sep;52(Pt 5):1621-8
references.pmid: 12361265
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: FEMS Microbiol Ecol. 2002 Dec 1;42(3):463-76
references.pmid: 19709305
references.citation: Appl Environ Microbiol. 2003 Jun;69(6):3580-92
references.pmid: 12788766
references.citation: Environ Microbiol. 2003 Jun;5(6):492-502
references.pmid: 12755716
references.citation: Appl Environ Microbiol. 2000 Feb;66(2):499-508
references.pmid: 10653710
references.citation: Int J Syst Bacteriol. 1996 Oct;46(4):1113-9
references.pmid: 8863444
references.citation: Antonie Van Leeuwenhoek. 1997 Jul;72(1):3-19
references.pmid: 9296259
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: J Mol Evol. 1981;17(6):368-76
references.pmid: 7288891
references.citation: Appl Environ Microbiol. 1993 Mar;59(3):695-700
references.pmid: 7683183
references.citation: Comput Appl Biosci. 1996 Dec;12(6):543-8
references.pmid: 9021275