title: Ultrasonic extraction followed by sulfuric acid silica gel cleanup for the determination of alpha-hexachlorocyclohexane enantiomers in biota samples.
abstract: Interest in the analysis of alpha-hexachlorocyclohexane (alpha-HCH) enantiomers as an alternative or complementary approach to elucidating isomer ratios of alpha/gamma-HCH has grown in recent years because it can provide useful information to evaluate the influence of different degradation and transformation processes. In this paper, a simple and rapid method for the determination of alpha-HCH enantiomers in biota samples is described. The method developed consists of ultrasonic extraction, sulfuric acid silica gel cleanup, solid-phase extraction (SPE) column fractionation, and final determination with chiral high-resolution gas chromatography. Ultrasonic extraction greatly shortens the extraction process time, and the sulfuric acid silica gel and SPE cleanup perfectly remove lipids and other interference compounds in the lipid-rich samples. The method is found to be simple, less time-consuming, and easy to operate, thus providing a useful alternative method to assess the enantioselective breakdown of alpha-HCH in biota system.
authors.affiliation: Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, P.R. China
authors.forename: Rusong
authors.initials: R
authors.lastname: Zhao
authors.forename: Shaogang
authors.initials: S
authors.lastname: Chu
authors.forename: Rongbiao
authors.initials: R
authors.lastname: Zhao
authors.forename: Xiaobai
authors.initials: X
authors.lastname: Xu
authors.forename: Xiufen
authors.initials: X
authors.lastname: Liu
mesh_terms: D044822:Biodiversity; D002850:Chromatography, Gel / Q000379:methods; D001556:Hexachlorocyclohexane / Q000032:analysis*; D010575:Pesticides / Q000032:analysis; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D013464:Sulfuric Acids / Q000737:chemistry* / Q000145:classification*; D014465:Ultrasonics
chemical_list: D010575:Pesticides; D013464:Sulfuric Acids; C040534:alpha-hexachlorocyclohexane; D001556:Hexachlorocyclohexane; D058428:Silica Gel; D012822:Silicon Dioxide; C033158:sulfuric acid