Hydrogenation of C-C double bonds in an ionic liquid reaction system using the obligate anaerobe, Sporomusa termitida.
Sporomusa termitida reduced caffeate (1mM) in anaerobic, two-liquid phase, reaction systems containing either tetradecane or 1-butyl-3-methylimidazolium hexafluorophosphate [[bmim][PF6]] (20% v/v). The initial rate and final product yield were 20 and 7% lower, respectively, in [bmim][PF6]. Since caffeate partitioned only into the aqueous phase, the lower rate cannot be attributed to mass transfer barriers. Therefore, [bmim][PF6] inhibited the biocatalyst, perhaps unsurprisingly since it is very polar and hydrolyses to produce HF.
Lenourry
Alix
A
School of Chemical Engineering, University of Manchester, P.O. Box 88, Manchester M60 1QD, UK.
Gardiner
John M
JM
Stephens
Gill
G
D000473:Alkanes / Q000737:chemistry / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000378:metabolism*; D001711:Biotransformation; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media; D006865:Hydrogenation; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors
C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D000473:Alkanes; D003470:Culture Media; D007093:Imidazoles; D012997:Solvents; C024713:n-tetradecane; D002244:Carbon