title: Radical species in DNA strand-cleavage caused by dihydropyrazines.
abstract: Dihydropyrazines (DHPs), which generate hydroxyl and carbon-centered radicals, cleaved DNA single-strand. It is new knowledge that DHPs were recently determined to produce 8-hydroxydeoxyguanosine. The remarkable increase in the DNA strand-cleavage activity upon the addition of Cu2+ suggests that the primary reactive species is carbon-centered radicals rather than the hydroxyl radical generated the initiation reaction. This proposal was in fair agreement with of the observed carbon-centered radical signal intensity, but an effect was not observed with the increase in the hydroxyl radical signal intensity.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Fokuoka, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kashige
authors.forename: Toru
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Takechi
authors.forename: Fumio
authors.initials: F
authors.lastname: Miake
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D004277:DNA, Single-Stranded / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism*; D011719:Pyrazines / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D004277:DNA, Single-Stranded; D005609:Free Radicals; D011719:Pyrazines