title: Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette.
authors.affiliation: Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Chen
authors.forename: Michael M
authors.initials: MM
authors.lastname: Gottesman
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D015245:Deoxyribonuclease BamHI / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D017353:Gene Deletion; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000235:genetics
chemical_list: C480744:ABCB5 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015245:Deoxyribonuclease BamHI; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific