title: Denitrifying activity of Xanthobacter autotrophicus strains isolated from a submerged fixed-film reactor.
abstract: Xanthobacter autotrophicus strains with the ability to reduce nitrate and nitrite to either nitrous oxide or molecular nitrogen were isolated from submerged fixed-film reactors. Isolated strains were Gram-negative rods able to grow on methanol, ethanol and sucrose. The yellow cellular pigmentation, pleomorphic appearance, and the presence of poly-beta-hydroxybutyrate granules suggest that the organisms might belong to the genus Xanthobacter. Comparison of 16S rDNA gene sequences demonstrated the affiliation of the strains to X. autotrophicus species. The results show that X. autotrophicus may play a role in inorganic nitrogen removal from a denitrifying submerged filter used for the treatment of contaminated groundwater. To our knowledge, no data on denitrifying activity in X. autotrophicus strains have been reported previously.
authors.affiliation: Group of Environmental Microbiology, Institute of Water Research, c/ Ramón y Cajal no 4, University of Granada, Spain.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Gómez
authors.forename: Belén
authors.initials: B
authors.lastname: Rodelas
authors.forename: Florentina
authors.initials: F
authors.lastname: Sáez
authors.forename: Clementina
authors.initials: C
authors.lastname: Pozo
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Martínez-Toledo
authors.forename: Ernesto
authors.initials: E
authors.lastname: Hontoria
authors.forename: Jesús
authors.initials: J
authors.lastname: González-López
mesh_terms: D018441:Biofilms; D019149:Bioreactors; D003470:Culture Media; D004867:Equipment Design; D009566:Nitrates / Q000378:metabolism*; D018508:Water Purification / Q000379:methods; D020584:Xanthobacter / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009566:Nitrates