title: Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence.
abstract: By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding proteins. In a mouse infection model, both mutants were attenuated in virulence, suggesting that M. tuberculosis encounters limiting phosphate concentrations during its intracellular life span.
authors.affiliation: Pasteur Institute of Brussels, Engelandstraat 642, B-1180-Brussels, Belgium.
authors.forename: Priska
authors.initials: P
authors.lastname: Peirs
authors.forename: Philippe
authors.initials: P
authors.lastname: Lefèvre
authors.forename: Samira
authors.initials: S
authors.lastname: Boarbi
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Olivier
authors.initials: O
authors.lastname: Denis
authors.forename: Martine
authors.initials: M
authors.lastname: Braibant
authors.forename: Kevin
authors.initials: K
authors.lastname: Pethe
authors.forename: Camille
authors.initials: C
authors.lastname: Locht
authors.forename: Kris
authors.initials: K
authors.lastname: Huygen
authors.forename: Jean
authors.initials: J
authors.lastname: Content
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D017353:Gene Deletion; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity*; D028044:Phosphate-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D014376:Tuberculosis / Q000382:microbiology* / Q000503:physiopathology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D028044:Phosphate-Binding Proteins; D010710:Phosphates
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