Pectinolytic systems of two aerobic sporogenous bacterial strains with high activity on pectin.
Strains Paenibacillus sp. BP-23 and Bacillus sp. BP-7, previously isolated from soil from a rice field, secreted high levels of pectinase activity in media supplemented with pectin. Production of pectinases in strain Paenibacillus sp. BP-23 showed catabolite repression, while in Bacillus sp. BP-7 production of pectin degrading enzymes was not negatively affected by glucose. The two strains showed lyase activities as the predominant pectinases, while hydrolase activity was very low. Analysis of Paenibacillus sp. BP-23 in SDS-polyacrylamide gels and zymograms showed five pectinase activity bands. The strict requirement of Ca(2+) for lyase activity of the strain indicates that correspond to pectate lyases. For Bacillus sp. BP-7, zymograms showed four bands of different size. The strain showed a Ca(2+) requirement for lyase activity on pectate but not on pectin, indicating that the pectinolytic system of Bacillus sp. BP-7 is comprised of pectate lyases and pectin lyases. The results show differences in pectin degrading systems between the two aerobic sporogenous bacterial strains studied.
Soriano
Margarita
M
Department of Microbiology, Faculty of Biology, University of Barcelona, Avenida Diagonal 645, 08028 Barcelona, Spain.
Diaz
Pilar
P
Pastor
F I Javier
FI
D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism*; D016937:Gram-Positive Endospore-Forming Rods / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010368:Pectins / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology; D013045:Species Specificity; D013696:Temperature
D010368:Pectins; D011133:Polysaccharide-Lyases; C027349:pectin lyase; C023911:pectate lyase
Biochim Biophys Acta. 2001 Dec 5;1568(2):162-70
11750764
Nature. 1970 Aug 15;227(5259):680-5
5432063
FEBS Lett. 1993 Dec 13;335(3):319-26
8262178
Eur J Biochem. 2003 Jul;270(13):2913-9
12823562
J Bacteriol. 1999 Mar;181(5):1652-63
10049400
FEBS Lett. 2000 Feb 11;467(2-3):333-6
10675564
Appl Environ Microbiol. 1994 Jun;60(6):2107-12
16349296
Annu Rev Microbiol. 1996;50:213-57
8905080
Eur J Biochem. 2000 Apr;267(8):2268-75
10759850
Microbiology. 2000 Jan;146 ( Pt 1):89-95
10658655
Biochimie. 1990 Sep;72 (9):689-95
2126210
J Bacteriol. 1997 Apr;179(8):2503-11
9098045
FEBS Lett. 1996 Dec 2;398(2-3):269-73
8977121
Microbiol Mol Biol Rev. 2001 Dec;65(4):497-522, table of contents
11729262
Appl Microbiol Biotechnol. 2002 Aug;59(4-5):409-18
12172603
Plant Cell. 1999 Jun;11(6):1081-92
10368179