Molecular mechanisms involved in the differential effects of sex steroids on the reproduction and infectivity of Taenia crassiceps.
The in vitro exposure of Taenia crassiceps cysticerci to 17-beta estradiol (E2) and progesterone (P4) stimulated their reproduction and infectivity. Testosterone (T4) and dihydrotestosterone (DHT) inhibited their reproduction and reduced their motility and infectivity. E2 and P4 increased, whereas T4 and DHT reduced, the expression of parasite c-fos and c-jun and DNA synthesis. In vitro exposure of cysticerci to sex steroids before their inoculation into recipient noninfected mice resulted in large parasite loads when pretreated with E2 and P4 and in smaller loads when pretreated with T4 and DHT To determine the possible molecular mechanisms by which sex steroids affect T. crassiceps, sex steroid receptors were amplified. Taenia crassiceps expressed estrogen receptors (both alpha and beta isoforms) and androgen receptors but no P4 receptors. These results demonstrate that sex steroids act directly on parasite reproduction by binding to a classic and specific sex steroid receptor on the parasite. The differential response of cysticerci to sex steroids may also be involved in their ability to grow faster in the murine female or feminized male host. This is the first report of direct sex steroid effects on the parasite possibly through sex steroid receptors in the cysticerci.
Escobedo
Galileo
G
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M., AP 70228, Mexico D.F. 04510, Mexico.
Larralde
Carlos
C
Chavarria
Anahí
A
Cerbón
Marco A
MA
Morales-Montor
Jorge
J
D000818:Animals; D018509:DNA, Helminth / Q000187:drug effects / Q000378:metabolism; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos / Q000187:drug effects / Q000502:physiology; D016758:Genes, jun / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011374:Progesterone / Q000494:pharmacology*; D018510:RNA, Helminth / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012100:Reproduction, Asexual / Q000187:drug effects; D013621:Taenia / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D013622:Taeniasis / Q000469:parasitology; D013739:Testosterone / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D018509:DNA, Helminth; D004967:Estrogens; D018510:RNA, Helminth; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D014316:Tritium; D013739:Testosterone; D011374:Progesterone; D013936:Thymidine