title: High-density DNA probe arrays for identification of staphylococci to the species level.
abstract: Rapid and accurate identification and speciation of staphylococci clinical isolates is important for predicting medical pathology. We evaluated the ability of a high-density DNA probe array based on 16S rDNA sequences to identify Staphylococcus species. Correct identification was observed for 185 out of the 201 strains (92%). Of the 33 tested species, the array was able to correctly identify 30 of them. The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies.
authors.affiliation: Genomic Research Laboratory, University Hospitals of Geneva Rue Micheli-du-Crest 24,1211 Geneva 14, Switzerland.
authors.forename: Sabine
authors.initials: S
authors.lastname: Couzinet
authors.forename: Corinne
authors.initials: C
authors.lastname: Jay
authors.forename: Christine
authors.initials: C
authors.lastname: Barras
authors.forename: René
authors.initials: R
authors.lastname: Vachon
authors.forename: Guy
authors.initials: G
authors.lastname: Vernet
authors.forename: Béatrice
authors.initials: B
authors.lastname: Ninet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jan
authors.forename: Marie-Antoinette
authors.initials: MA
authors.lastname: Minazio
authors.forename: Patrice
authors.initials: P
authors.lastname: Francois
authors.forename: Daniel
authors.initials: D
authors.lastname: Lew
authors.forename: Alain
authors.initials: A
authors.lastname: Troesch
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase