title: Expression of Cu/Zn SOD protein is suppressed in hsp 70.1 knockout mice.
abstract: Heat shock proteins (HSPs) are known to protect cells from oxidative stress and other types of injuries. We previously reported the neuroprotective effect of HSP70 following cerebral ischemia and reperfusion using hsp 70.1 knockout (KO) mice. However, the precise role of HSP70 in neuroprotection has not been established yet. The purpose of this study was to investigate the relationship between HSP70 and antioxidant enzymes using hsp 70.1 KO mice. The activities of both SOD-1 and SOD-2 were significantly decreased in hsp 70.1 KO mice than in the wild type (WT) littermates. SOD-1 protein level in the hsp 70.1 KO mice was lower than that of WT. We speculate that HSP70 might be involved in regulation of expression of SOD-1 at the level of transcription or by post-transcriptional modification.
authors.affiliation: College of Nursing, Seoul National University, Seoul 110-744, Korea.
authors.forename: Smi
authors.initials: S
authors.lastname: Choi
authors.forename: Kyung Ae
authors.initials: KA
authors.lastname: Park
authors.forename: Hee Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Myoung Sook
authors.initials: MS
authors.lastname: Park
authors.forename: Joung Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyoung Chan
authors.initials: KC
authors.lastname: Park
authors.forename: Manho
authors.initials: M
authors.lastname: Kim
authors.forename: Seung-Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Seo
authors.forename: Byung-Woo
authors.initials: BW
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D015536:Down-Regulation; D018390:Gene Targeting; D005979:Glutathione Peroxidase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D018840:HSP70 Heat-Shock Proteins; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2; C081884:heat-shock protein 70.1; C000606291:Sod1 protein, mouse