Fluorescence quenching mechanism of a polyphenylene polyelectrolyte with other macromolecules: cytochrome c and dendrimers.
This study investigates the fluorescence quenching of a polyphenyl based polyelectrolyte by positively charged macromolecules (proteins and dendrimers). This work shows that the fluorescence quenching of the dendrimer materials does not involve energy transfer or electron transfer but is correlated to the overall charge on the dendrimer and its size. The quenching is hypothesized to result from conformational changes that occur upon binding the polyelectrolyte to the protein or dendrimer. This mechanism is qualitatively different from that invoked for small-molecule analytes.
Liu
Min
M
Chemistry Department, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, USA.
Kaur
Palwinder
P
Waldeck
D H
DH
Xue
Cuihua
C
Liu
Haiying
H
D045304:Cytochromes c / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D013057:Spectrum Analysis
D050091:Dendrimers; D004573:Electrolytes; D011108:Polymers; D045304:Cytochromes c; C041325:polyphenylene sulfide