title: mCD24 regulates proliferation of neuronal committed precursors in the subventricular zone.
abstract: We previously showed that deletion of the cell surface molecule mCD24 resulted in an increased proliferation in adult subventricular zone (SVZ). Here, we report an increased PSA-NCAM+/TuJ1- population in the mCD24-/- in vivo SVZ as well as in vitro neurospheres. Isolated in vitro, these cells were able to generate neurospheres. Proliferation studies, using BrdU incorporation, showed an increased proliferation in P7 mCD24-/- SVZ and neurospheres. Using electron microscopy, the same cell types were identified in the in vivo SVZ as well as in vitro neurospheres from the WT and mCD24-/- mice. In mixed neurospheres, formed with WT and EGFP/KO cells (enhanced green fluorescent protein mCD24-/-), the WT environment was able to control the proliferation rate of the mCD24-/- cells, but was unable to regulate their differentiation. We concluded that mCD24 acts cell nonautonomously to regulate transit-amplifying cells proliferation and/or differentiation.
authors.affiliation: Neurogenèse et Morphogenèse dans le Développement et chez l'Adulte/Institut de Biologie du Développement de Marseille, Centre National de la Recherche Scientifique, Marseilles, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Nieoullon
authors.forename: Richard
authors.initials: R
authors.lastname: Belvindrah
authors.forename: Geneviève
authors.initials: G
authors.lastname: Rougon
authors.forename: Geneviève
authors.initials: G
authors.lastname: Chazal
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051927:CD24 Antigen; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins; D020547:Lateral Ventricles / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D019007:P-Selectin / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D008562:Membrane Glycoproteins; D039842:Neural Cell Adhesion Molecule L1; D019007:P-Selectin; D012794:Sialic Acids; D014404:Tubulin; C462941:beta3 tubulin, mouse; C103469:polysialyl neural cell adhesion molecule; D049452:Green Fluorescent Proteins