title: Establishment and characterization of the permanent human cell line C3842 derived from a secondary chondrosarcoma in Ollier's disease.
abstract: The permanent human cell line C3842 was established from a secondary chondrosarcoma in a typical case of Ollier's disease. In the present study, we analyzed the morphological, cytogenetic and molecular biological characteristics of the cultured cells in comparison with the original tumor and investigated the invasion properties of the tumor model using functional imaging of proteolysis, matrigel assay and chick chorioallantoic membrane assay. C3842 cells exhibit the typical features of malignant cartilage tumor cells in vitro, including the expression of collagen types II, IX, XI and aggrecan. The proteolytic ability of C3842 cells is attributed to the expression of several proteases, such as cathepsin B, urokinase plasminogen activator and matrix-metalloproteinase-2, which enable the cells to degrade collagen type I and to permeate matrigel matrix. In accordance with the biological features in vivo, C3842 cells are not able to invade through the epithelium of the chick chorioallantoic membrane. In conclusion, the cell line C3842 provides the first model of a secondary chondrosarcoma in Ollier's disease in vitro, which is characterized by distinct features of such malignant cartilage tumors.
authors.affiliation: Department of Pathology, Otto-von-Guericke-University, Leipziger Strasse 44, 39120 Magdeburg, Germany. kalinski@medizin.uni-magdeburg.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Kalinski
authors.forename: Sabine
authors.initials: S
authors.lastname: Krueger
authors.forename: Antje-Friederike
authors.initials: AF
authors.lastname: Pelz
authors.forename: Peter
authors.initials: P
authors.lastname: Wieacker
authors.forename: Roland
authors.initials: R
authors.lastname: Hartig
authors.forename: Martin
authors.initials: M
authors.lastname: Röpke
authors.forename: Regine
authors.initials: R
authors.lastname: Schneider-Stock
authors.forename: Frank
authors.initials: F
authors.lastname: Dombrowski
authors.forename: Albert
authors.initials: A
authors.lastname: Roessner
mesh_terms: D000328:Adult; D000818:Animals; D001859:Bone Neoplasms / Q000150:complications* / Q000473:pathology / Q000648:ultrastructure; D045744:Cell Line, Tumor / Q000502:physiology* / Q000648:ultrastructure; D002813:Chondrosarcoma / Q000150:complications* / Q000473:pathology / Q000648:ultrastructure; D049033:Chorioallantoic Membrane; D002869:Chromosome Aberrations; D004687:Enchondromatosis / Q000150:complications*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009361:Neoplasm Invasiveness; D018807:Polymorphism, Single-Stranded Conformational; D020133:Reverse Transcriptase Polymerase Chain Reaction
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