title: Human endothelial cell interaction with biomimetic surfactant polymers containing Peptide ligands from the heparin binding domain of fibronectin.
abstract: Biomimetic materials that mimic the extracellular matrix (ECM) provide a means to control cellular functions such as adhesion and growth, which are vital to successful engineering of tissue-incorporated biomaterials. Novel "ECM-like" biomimetic surfactant polymers consisting of a poly(vinyl amine) backbone with pendant cell-adhesive peptides derived from one of the heparin-binding domains of fibronectin were developed to improve endothelial cell adhesion and growth on vascular biomaterials. Heparin-binding peptide (HBP) sequences, alone and in combination with RGD peptides, were examined for their ability to promote human pulmonary artery endothelial cell (HPAEC) adhesion and growth (HBP1, WQPPRARI; HBP2, SPPRRARVT; HBP1:RGD; and HBP2:RGD) and compared with cell adhesion and growth on fibronectin and on negative control polymer surfaces in which alanines were substituted for the positively charged arginine residues in the two peptides. The results showed that HPAECs adhered and spread equally well on all HBP-containing polymers and the positive fibronectin control, showing similar stress fiber and focal adhesion formation. However, the HBP alone was unable to support long-term HPAEC growth and survival, showing a loss of focal adhesions and cytoskeletal disorganization by 24 h after seeding. With the addition of RGD, the surfaces behaved similarly or better than fibronectin. The negative control polymers showed little to no initial cell attachment, and the addition of soluble heparin to the medium reduced initial cell adhesion on both the HBP2 and HBP2:RGD surfaces. These results indicate that the HBP surfaces promote initial HPAEC adhesion and spreading, but not long-term survival.
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106-7207, USA.
authors.forename: Sharon
authors.initials: S
authors.lastname: Sagnella
authors.forename: Eric
authors.initials: E
authors.lastname: Anderson
authors.forename: Naomi
authors.initials: N
authors.lastname: Sanabria
authors.forename: Roger E
authors.initials: RE
authors.lastname: Marchant
authors.forename: Kandice
authors.initials: K
authors.lastname: Kottke-Marchant
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology; D005109:Extracellular Matrix / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry; D006801:Humans; D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D011651:Pulmonary Artery / Q000166:cytology; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D008024:Ligands; D009842:Oligopeptides; D011108:Polymers; D013501:Surface-Active Agents; C047981:arginyl-glycyl-aspartic acid; D006493:Heparin
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