The protective effect of selenium on ipsilateral and contralateral testes in testicular reperfusion injury.
This study was designed to investigate the effect of selenium on ipsilateral and contralateral testicular damage after unilateral testicular torsion/detorsion (T/D). Thirty-two male rats were divided into four groups, each containing eight rats. Torsion was created by rotating the right testis 720 degrees in a clockwise direction. Group 1 underwent sham operation to determine basal values for biochemical and histopathological evaluation. Sham operation was performed in group 2, and sodium selenate (0.2 mg/kg) was given intraperitoneally. Group 3 served as a T/D group, receiving 4-h torsion and 4-h detorsion. Similarly, in group 4 sodium selenate (0.2 mg/kg) was injected intraperitoneally 20 min before detorsion. Bilateral orchiectomies were performed for measurement of tissue malondialdehyde (MDA) levels and superoxide dismutase (SOD) activities and histopathologic examination. The results were compared statistically. The highest MDA and the lowest SOD values were determined in both testes in group 3. There were statistically significant differences in MDA levels and SOD activities in group 3 compared with group 4. Specimens from group 3 had a significantly greater histologic injury than other groups. These results suggest that ischemia-reperfusion injury occurred in both testes after unilateral testicular T/D and that selenium administration before detorsion prevents reperfusion injury in testicular torsion.
Avlan
Dinçer
D
Department of Pediatric Surgery, School of Medicine, Mersin University, Zeytinlibahçe 33070, Mersin, Turkey. dinceravlan@yahoo.com
Erdouğan
Kubilay
K
Cimen
Burak
B
Düşmez Apa
Duygu
D
Cinel
Ismail
I
Aksöyek
Selim
S
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D012643:Selenium / Q000627:therapeutic use*; D013086:Spermatic Cord Torsion / Q000601:surgery*; D013482:Superoxide Dismutase / Q000032:analysis; D013737:Testis / Q000098:blood supply
D000975:Antioxidants; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D012643:Selenium
J Urol. 1994 Apr;151(4):1070-2
8126796
Arch Surg. 2001 Oct;136(10):1201-7
11585516
Urology. 1994 Sep;44(3):413-8
8073557
Pharmacol Res. 2003 Aug;48(2):209-15
12798674
Lancet. 1984 Dec 15;2(8416):1357-60
6150364
J Urol. 1997 Mar;157(3):1149-54
9072560
Med Klin (Munich). 1997 Sep 15;92 Suppl 3:10-1
9417485
Lancet. 1981 Oct 3;2(8249):723-5
6116860
Crit Rev Clin Lab Sci. 1989;27(6):483-541
2690856
Crit Care Med. 2000 Apr;28(4 Suppl):N100-4
10807322
Pharmacol Res. 2002 Aug;46(2):171-5
12220957
J Pediatr Surg. 1999 Jun;34(6):1004-6
10392923
J Urol. 1997 Jan;157(1):340-5
8976294
J Clin Lab Anal. 1996;10(1):17-20
8926562
Semin Liver Dis. 1996 Feb;16(1):31-8
8723321
J Pediatr Surg. 2000 Jan;35(1):101-3
10646784
Gen Pharmacol. 1995 Dec;26(8):1669-72
8745154
Br J Urol. 1989 May;63(5):522-4
2731010
Andrologia. 1984 May-Jun;16(3):239-43
6431848
N Engl J Med. 1985 Jan 17;312(3):159-63
2981404
Gen Pharmacol. 1994 Nov;25(7):1493-7
7896065
Arch Androl. 1987;19(3):237-41
3504134
Free Radic Biol Med. 2000 Mar 15;28(6):979-86
10802230
J Pathol. 2000 Feb;190(3):255-66
10685060
Transplant Proc. 2001 Feb-Mar;33(1-2):974-5
11267152
Kidney Int. 1990 Dec;38(6):1109-17
2074654
J Androl. 1986 Jan-Feb;7(1):23-31
3944017
J Pediatr Surg. 2003 Nov;38(11):1621-7
14614712
Am J Surg. 1991 Apr;161(4):488-503
2035771
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Br J Urol. 1982 Dec;54(6):765-8
7150939
J Urol. 1982 Dec;128(6):1343-8
7154207
Clin Chim Acta. 2003 Aug;334(1-2):163-71
12867288
J Androl. 1985 Jan-Feb;6(1):65-72
3972720
J Surg Res. 1993 Nov;55(5):553-8
8231175
Chem Phys Lipids. 1987 Jul-Sep;44(2-4):255-76
3311419
FEBS Lett. 1994 Aug 8;349(3):392-6
8050602