The moderately halophilic bacterium Halomonas maura is a free-living diazotroph.
Halomonas maura is a moderately halophilic bacterium which lives in saline soils and synthesises an exopolysaccharide known as mauran. Strain S-31T grew in a nitrogen-free medium under an N2 atmosphere; the acetylene reduction assay proved positive under specific conditions. We identified the nifH gene in this strain by using degenerate oligonucleotides designed from highly preserved gene sequences obtained from the alignment of a large number of nifH sequences from different microorganisms. Our results lead us to conclude that H. maura is capable of fixing nitrogen under microaerobic conditions.
Argandoña
Montserrat
M
Departamento de Microbiología, Facultad de Farmacia, Campus Universitario de Cartuja s/n., 18071 Granada, Spain.
Fernández-Carazo
Rafael
R
Llamas
Inmaculada
I
Martínez-Checa
Fernando
F
Caba
Juan Manuel
JM
Quesada
Emilia
E
del Moral
Ana
A
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D020632:Halomonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D012988:Soil Microbiology
D004269:DNA, Bacterial; D010088:Oxidoreductases; C029130:nitrogenase reductase