title: Serum, salivary and tissue proteomics for discovery of biomarkers for head and neck cancers.
abstract: Initial clinically oriented applications of emerging proteomic technologies that aim to identify biomarkers for head and neck squamous cell carcinoma diagnostics have yielded promising results. The development of new proteomic diagnostics remains critical for the early detection of head and neck squamous cell carcinoma at more treatable stages. Prognostic markers for disease recurrence and treatment sensitivities are also required. In this overview of current biomarker identification strategies for head and neck squamous cell carcinoma, different combinations of mass spectrometry platforms, laser capture microscopy and 2D gel electrophoresis procedures are summarized as applied to readily available clinical specimens (tissue, blood and saliva). Issues related to assay reproducibility, management of large data sets and future improvements in clinical proteomics are also addressed.
authors.affiliation: Eastern Virginia Medical School, Center for Biomedical Proteomics, Department of Microbiology & Molecular Cell Biology, Norfolk, VA 23507, USA. drakerr@evms.edu
authors.forename: Richard R
authors.initials: RR
authors.lastname: Drake
authors.forename: Lisa H
authors.initials: LH
authors.lastname: Cazare
authors.forename: O John
authors.initials: OJ
authors.lastname: Semmes
authors.forename: J Trad
authors.initials: JT
authors.lastname: Wadsworth
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D020543:Proteome