HPA binding and metastasis formation of human breast cancer cell lines transplanted into severe combined immunodeficient (scid) mice.
Six human breast cancer cell lines were injected subcutaneously into scid mice and their in vivo growth behaviour and HPA binding pattern were analysed. Furthermore, the role of HPA binding glycoconjugates concerning the adhesion to endothelial cells in vitro was investigated. Four of the tested cell lines engrafted in the scid mouse model but they showed considerable variations concerning their growth behaviour, their metastatic potential and their HPA binding pattern. HPA inhibited adhesive interactions between cell lines derived from metatstatic sources and tumour necrosis factor (TNF)alpha stimulated endothelial cells. The transplantation of HPA defined breast cancer cell lines into scid mice is a useful animal model for the research of breast cancer and its metastasis. The HPA binding glycoconjugates appear to be associated with adhesive interactions between metastasising tumour cells and endothelial cells.
Valentiner
Ursula
U
Institute for Anatomy II: Experimental Morphology, University Hospital Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany. valentin@uke.uni-hamburg.de
Hall
Debbie M S
DM
Brooks
Susan A
SA
Schumacher
Udo
U
D000116:Acetylgalactosamine / Q000378:metabolism; D000818:Animals; D001940:Breast / Q000166:cytology; D002448:Cell Adhesion; D004727:Endothelium / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C038095:Helix lectin; D037102:Lectins; D014409:Tumor Necrosis Factor-alpha; D000116:Acetylgalactosamine