High-density DNA probe arrays for identification of staphylococci to the species level.
Rapid and accurate identification and speciation of staphylococci clinical isolates is important for predicting medical pathology. We evaluated the ability of a high-density DNA probe array based on 16S rDNA sequences to identify Staphylococcus species. Correct identification was observed for 185 out of the 201 strains (92%). Of the 33 tested species, the array was able to correctly identify 30 of them. The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies.
Couzinet
Sabine
S
Genomic Research Laboratory, University Hospitals of Geneva Rue Micheli-du-Crest 24,1211 Geneva 14, Switzerland.
Jay
Corinne
C
Barras
Christine
C
Vachon
René
R
Vernet
Guy
G
Ninet
Béatrice
B
Jan
Isabelle
I
Minazio
Marie-Antoinette
MA
Francois
Patrice
P
Lew
Daniel
D
Troesch
Alain
A
Schrenzel
Jacques
J
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase