An ultrasensitive nucleic acid biosensor based on the catalytic oxidation of guanine by a novel redox threading intercalator.
An ultrasensitive nucleic acid biosensor for the direct detection of attomoles nucleic acid in 1.0-5.0 [corrected] microl droplets is described which can be used for detection of cancer marker genes in mRNA extracted from human breast tissues without a RT-PCR step.
Tansil
Natalia C
NC
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore, 138669, Singapore. zqgao@ibn.a-star.edu.sg.
Xie
Fang
F
Xie
Hong
H
Gao
Zhiqiang
Z
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D004247:DNA / Q000032:analysis / Q000235:genetics; D016158:Genes, p53; D006147:Guanine / Q000737:chemistry*; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry*; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry*; D009696:Nucleic Acids / Q000032:analysis*; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D007364:Intercalating Agents; D009281:Naphthalenes; D009696:Nucleic Acids; D009942:Organometallic Compounds; C518125:PIND-Ru; D006147:Guanine; D004247:DNA