title: Use of hand held photopolymerizer to photoinactivate Streptococcus mutans.
abstract: OBJECTIVE
  The main focus of this research was to investigate the photodynamic therapy (PDT), in vitro, acting on Streptococcus mutans and fibroblasts. A hand held photopolymerizer (HHP) and a classical photosensitizer (Rose Bengal) were used to induce photodynamic response.
  
  
  METHODS
  S. mutans and fibroblast were treated with different concentrations of Rose Bengal (0-50 microM) irradiated with light (400-500 nm) for different time periods (0-40s) and then cell viability was evaluated.
  
  
  RESULTS
  It was observed that the light (per se) is not toxic and in the dark Rose Bengal is toxic to the cells tested only at concentrations above 2.5 microM. Under light exposure concentrations of Rose Bengal above 0.5 microM all S. mutans were killed with no cytotoxic effects to fibroblasts.
  
  
  CONCLUSIONS
  For the purpose of this work, the photoactivation of Rose Bengal, using the HHP, inactivated the bacteria without affecting the fibroblast viability.
authors.affiliation: Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, FFCLRP-USP, 14040-901 Ribeirão Preto, SP, Brazil.
authors.forename: Tony P
authors.initials: TP
authors.lastname: Paulino
authors.forename: Karina F
authors.initials: KF
authors.lastname: Ribeiro
authors.forename: Geraldo
authors.initials: G
authors.lastname: Thedei
authors.forename: Antônio C
authors.initials: AC
authors.lastname: Tedesco
authors.forename: Pietro
authors.initials: P
authors.lastname: Ciancaglini
mesh_terms: D001431:Bacteriological Techniques; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008027:Light; D010778:Photochemotherapy / Q000295:instrumentation; D017319:Photosensitizing Agents / Q000633:toxicity*; D012395:Rose Bengal / Q000633:toxicity*; D013295:Streptococcus mutans / Q000187:drug effects / Q000528:radiation effects*; D013997:Time Factors
chemical_list: D017319:Photosensitizing Agents; D012395:Rose Bengal