[Analysis of ant oil extracted with supercritical CO2 fluid from Polyrhachis vicina by GC-MS].
OBJECTIVE
To study the optimum extraction parameters and components on ant oil from Polyrhachis vicina.


METHODS
The optimum condifious for supercritical CO2 fluid extraction (SFE-CO2), were investigated with orthogonal design, GC-MS was applied for analyzing. The components and their contents in the ant oil were analyzed by GC-MS, and the contents of lead, zinc and manganese in the oil were determined by ICP-AES.


RESULTS
The optimum extraction parameters were achieved, temperature of 50 degrees C, pressure of 30 MPa and time of 2 hours. The extracting yield of the ant volatile oil was 11.4% - 14.3%. 51 Constituents were identified including 9-octadecenoic acid, ethyl oleate, cholesterol, n- Hexadecanoic acid, etc, and the content of various constituents was determined by orea normalization. The oil contained unsaturated fatty acid of 64.6%, lead of 0.80 microg x g(-1), zinc of 0.54 microg x g(-1) and manganese of 0.15 microg x g(-1).


CONCLUSIONS
The method showes advantages including faster and efficient of extraction, good quality and no solvent residues in the oil.
Shen
Li-Rong
LR
Department of Food Science & Nutrition, Zhejiang University, Hangzhou 310029, China. shenlirong@zju.edu.cn
Ren
Yu-Cui
YC
Feng
Feng-Qin
FQ
Du
Meng-Hao
MH
D000818:Animals; D001000:Ants / Q000737:chemistry*; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007854:Lead / Q000032:analysis; D008345:Manganese / Q000032:analysis; D008419:Materia Medica / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D015032:Zinc / Q000032:analysis
D005231:Fatty Acids, Unsaturated; D008419:Materia Medica; D009822:Oils, Volatile; D002245:Carbon Dioxide; D007854:Lead; D008345:Manganese; D015032:Zinc