title: [Effect of gardenin on gene expression profile in brain of rats with focal cerebral ischemia].
abstract: OBJECTIVE
  To explore the pharmacologic mechanism of gardenin in treating cerebral ischemia, by studying its effect on gene expression profile in brain of rats with focal cerebral ischemia (FCI).
  
  
  METHODS
  Total RNAs were isolated from rats with FCI and those treated with gardenin. The mRNAs were reversely transcribed to cDNA with incorporation of fluorescent Cy5- or Cy3-dUTP to prepare hybridization probes. The PCR products of 4096 genes were spotted on the chip after a serial treatment. The mixed probes were hybridized to the cDNA microarray. Axon Genepix 4000B and GenePixPro 3.0 software were used to scan and analyze the fluorescent signals.
  
  
  RESULTS
  In the group treated with gardenin, there were 70 genes had expression profiles different to that in the model group in the focal cerebral ischemic brain tissue, in which 68 were up-regulated and 2 down-regulated.
  
  
  CONCLUSIONS
  Gardenin has regulatory effect on the gene expression in rats with focal cerebral ischemia, which elucidates part of the pharmacologic mechanism of Qingkailing in molecular level.
authors.affiliation: Institute of Life Science, Beijing Normal University, Beijing 100875. xiaoyanzhang@hotmail.com
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Zhan-jun
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Kang
authors.initials: K
authors.lastname: Ying
authors.forename: Ming-xue
authors.initials: MX
authors.lastname: Zuo
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011725:Pyridines; C074986:gardenine