title: Accumulation of miR-155 and BIC RNA in human B cell lymphomas.
abstract: We show that the microRNA miR-155 can be processed from sequences present in BIC RNA, a spliced and polyadenylated but non-protein-coding RNA that accumulates in lymphoma cells. The precursor of miR-155 is likely a transient spliced or unspliced nuclear BIC transcript rather than accumulated BIC RNA, which is primarily cytoplasmic. By using a sensitive and quantitative assay, we find that clinical isolates of several types of B cell lymphomas, including diffuse large B cell lymphoma (DLBCL), have 10- to 30-fold higher copy numbers of miR-155 than do normal circulating B cells. Similarly, the quantities of BIC RNA are elevated in lymphoma cells, but ratios of the amounts of the two RNAs are not constant, suggesting that the level of miR-155 is controlled by transcription and processing. Significantly higher levels of miR-155 are present in DLBCLs with an activated B cell phenotype than with the germinal center phenotype. Because patients with activated B cell-type DLBCL have a poorer clinical prognosis, quantification of this microRNA may be diagnostically useful.
authors.affiliation: Department of Biomolecular Chemistry, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706, USA.
authors.forename: Peggy S
authors.initials: PS
authors.lastname: Eis
authors.forename: Wayne
authors.initials: W
authors.lastname: Tam
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
authors.forename: Amy
authors.initials: A
authors.lastname: Chadburn
authors.forename: Zongdong
authors.initials: Z
authors.lastname: Li
authors.forename: Mario F
authors.initials: MF
authors.lastname: Gomez
authors.forename: Elsebet
authors.initials: E
authors.lastname: Lund
authors.forename: James E
authors.initials: JE
authors.lastname: Dahlberg
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D035683:MicroRNAs / Q000032:analysis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000032:analysis / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D022661:RNA, Untranslated
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