Screening of LPS-specific peptides from a phage display library using epoxy beads.
The selection of identical or highly homologous peptides from phage display combinatorial peptide libraries has been unsuccessful in biopanning experiments using microtiter plates. In the present study, by biopanning on LPS-conjugated epoxy beads, we repeatedly enriched clones encoding AWLPWAK and NLQEFLF. These peptides were found to interact with the polysaccharide moiety of LPS, which is highly variable among gram negative bacterial species. In addition, phages encoding these peptides preferentially bound to the LPS of Salmonella family. AWLPWAK-conjugated beads absorbed Salmonella enteritidis from solution and showed a preference for S. enteritidis over Escherichia coli. In summary, this study shows for the first time that a peptide screened from phage displays of combinatorial peptide libraries can be synthesized on beads and be used practically to concentrate bacterial cells from solution.
Kim
Yun-Gon
YG
Program in Biochemical Engineering and Biotechnology, Seoul National University, Korea.
Lee
Chang-Soo
CS
Chung
Woo-Jae
WJ
Kim
Eun-Mi
EM
Shin
Dong-Sik
DS
Rhim
Jung-Hyo
JH
Lee
Yoon-Sik
YS
Kim
Byung-Gee
BG
Chung
Junho
J
D001435:Bacteriophages / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D004853:Epoxy Resins / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D007118:Immunoassay; D008070:Lipopolysaccharides / Q000737:chemistry; D008856:Microscopy, Fluorescence; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D011485:Protein Binding; D012477:Salmonella enteritidis / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance
D004853:Epoxy Resins; D008070:Lipopolysaccharides; D019151:Peptide Library; D010455:Peptides; D011134:Polysaccharides