Grouping myxococci (Corallococcus) strains by Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI TOF) mass spectrometry: comparison with gene sequence phylogenies.
Nine Corallococcus isolates and three type strains of Corallococcus species were characterized by Intact Cell Mass Spectrometry using Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) mass spectrometry. The resulting phenetic clustering was compared to the phylogenetic grouping based upon sequences of two housekeeping genes. The three dendrograms of relatedness resembled each other in that the isolates were highly similar to the type strains of Corallococcus exiguus and Corallococcus coralloides, while Corallococcus macrosporus and Myxococcus xanthus were more distantly related. While certain pairs of organisms were recovered by spectrometry and genes sequence analysis, others were detected by two of the three approaches. The degree of similarity determined by sequence analysis of the two genes was not higher than that revealed by MALDI-TOF analysis. The results show that the spectral profile, consisting of about 25 to 45 masses ranging between 2 and 20 kDa, have indeed taxonomic significance, confirming literature data that ribosomal proteins and certain housekeeping proteins are responsible for the masses obtained. Provided the availability of a database of type strains, MALDI-TOF analysis of unknown strains appears to be a rapid and inexpensive method to taxonomically cluster environmental isolates, expanding the spectrum to strains other than those of medical importance predominantly investigated so far.
Stackebrandt
Erko
E
DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Mascherode Weg 1b, 38124 Braunschweig, Germany. erko@dsmz.de
Päuker
Orsola
O
Erhard
Marcel
M
D015373:Bacterial Typing Techniques / Q000379:methods*; D005798:Genes, Bacterial; D009231:Myxococcales / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D016941:Myxococcus xanthus / Q000737:chemistry / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
J Microbiol Methods. 2002 Feb;48(2-3):107-15
11777561
Rapid Commun Mass Spectrom. 1998;12(8):456-64
9586233
Mass Spectrom Rev. 2001 Jul-Aug;20(4):172-94
11835305
Proc Natl Acad Sci U S A. 2002 May 14;99(10):7039-44
11983869
Mass Spectrom Rev. 2001 Jul-Aug;20(4):157-71
11835304
Rapid Commun Mass Spectrom. 1996;10(10):1227-32
8759332
Nat Biotechnol. 1997 Sep;15(9):906-9
9306409
Rapid Commun Mass Spectrom. 2000;14(10):911-7
10825256
FEMS Microbiol Rev. 2000 Apr;24(2):193-219
10717314
Anal Chem. 1999 Aug 1;71(15):3226-30
10450164
Am Clin Lab. 2000 Apr;19(3):20-1
11010587
J Theor Biol. 1965 Mar;8(2):357-66
5876245
Anal Chem. 1999 Jul 15;71(14):2732-8
10424165
Rapid Commun Mass Spectrom. 1997;11(17):1900-8
9404039
J Microbiol Methods. 2002 Feb;48(2-3):117-26
11777562
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9459-63
1384053
Int J Syst Bacteriol. 1999 Jul;49 Pt 3:1255-62
10425789
Anal Chem. 2001 Feb 15;73(4):746-50
11248887
Int J Syst Bacteriol. 1998 Jul;48 Pt 3:813-9
9734035
Appl Environ Microbiol. 1995 Mar;61(3):1104-9
7793912
J Bacteriol. 1995 Mar;177(5):1402-4
7868619
Anal Chem. 2002 Nov 1;74(21):5487-91
12433077
Anal Biochem. 1999 Apr 10;269(1):105-12
10094780
Nat Biotechnol. 1996 Nov;14(11):1584-6
9634826
J Med Microbiol. 2000 Mar;49(3):295-300
10707951