Response of blood endothelin-1 and nitric oxide activity in duodenal ulcer patients undergoing Helicobacter pylori eradication.
OBJECTIVE
To investigate the effect of Helicobacter pylori eradication on endothelin-1 (ET-1) and nitric oxide (NO) in duodenal ulcer (DU) patients.


METHODS
Sixty-six H pylori-infected active DU patients were consecutively enrolled to receive one-week triple therapy (rabeprazole, amoxicillin and metronidazole) and then one-month rabeprazole therapy. They were asked back to determine ulcer and H pylori status using endoscopy one month later. Thirty-seven healthy controls (H pylori +/-: 17/20) were enrolled for comparison. Blood samples were collected in each visit to measure plasma ET-1 and nitrate/nitrite levels using an enzyme immunoassay kit.


RESULTS
Sixty DU patients finished trial per protocol. The ulcer healing and H pylori-eradication rates were 86.7% and 83.3%, respectively. Plasma ET-1 level in DU patients was higher than that of H pylori-negative and positive controls (3.59+/-0.96 vs 0.89+/-0.54 vs 0.3+/-0.2 pg/mL, P<0.01), while nitrate/nitrite levels among them were also significantly different (8.55+/-0.71 vs 5.27+/-0.68 vs 6.39+/-0.92 mumol/L, P<0.05). H pylori eradication diminished ET-1 levels (3.64+/-0.55 vs 2.64+/-0.55 pg/mL, P<0.01) but elevated nitrate/nitrite level (8.16+/-0.84 vs 11.41+/-1.42 mumol/L, P<0.05).


CONCLUSIONS
Both plasma ET-1 and nitrate/nitrite levels increase in active DU patients. After an effective H pylori eradication, DU healing is associated with diminished blood ET-1 level and elevated nitrate/nitrite level.
Chang
Full-Young
FY
Chief, Division of Gastroenterology, Taipei Veterans General Hospital, 201, Section 2, Shih-Pai Road, Taipei 112, Taiwan, China. changfy@vghtpe.gov.tw
Chen
Chih-Yen
CY
Lu
Ching-Liang
CL
Luo
Jiing-Chyuan
JC
Lu
Rei-Hwa
RH
Lee
Shou-Dong
SD
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000658:Amoxicillin / Q000008:administration & dosage; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D000890:Anti-Infective Agents / Q000008:administration & dosage; D000897:Anti-Ulcer Agents / Q000008:administration & dosage; D001562:Benzimidazoles / Q000008:administration & dosage; D015415:Biomarkers / Q000097:blood; D004359:Drug Therapy, Combination; D004381:Duodenal Ulcer / Q000097:blood* / Q000382:microbiology; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D016481:Helicobacter Infections / Q000097:blood* / Q000150:complications / Q000188:drug therapy; D016480:Helicobacter pylori / Q000187:drug effects*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage; D008875:Middle Aged; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000097:blood*; D009573:Nitrites / Q000097:blood; D009853:Omeprazole / Q000008:administration & dosage / Q000031:analogs & derivatives*; D064750:Rabeprazole
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000897:Anti-Ulcer Agents; D001562:Benzimidazoles; D015415:Biomarkers; D019332:Endothelin-1; D009566:Nitrates; D009573:Nitrites; D008795:Metronidazole; D009569:Nitric Oxide; D064750:Rabeprazole; D000658:Amoxicillin; D009853:Omeprazole
Dig Dis Sci. 1997 Feb;42(2):314-8
9052512
Dig Dis Sci. 1998 Feb;43(2):235-40
9512112
J Physiol Pharmacol. 1996 Mar;47(1):131-5
8777293
Helicobacter. 2003 Apr;8(2):95-104
12662376
Regul Pept. 1998 Jan 2;73(1):43-50
9537672
Lancet. 1999 Jan 9;353(9147):133-8
10023915
J Physiol Pharmacol. 1997 Jun;48 Suppl 3:1-56
9282334
Scand J Gastroenterol. 1998 Apr;33(4):386-93
9605260
J Gastroenterol Hepatol. 2003 Nov;18(11):1250-6
14535981
Circulation. 1994 Mar;89(3):1320-2
8124823
Am J Gastroenterol. 1994 Sep;89(9):1511-4
8079929
Scand J Gastroenterol. 1992 Apr;27(4):257-62
1375389
Am J Physiol. 1993 Sep;265(3 Pt 1):G491-8
8214072
J Clin Pathol. 2001 Apr;54(4):293-7
11304846
Eur J Clin Invest. 1998 Jul;28(7):511-9
9726029
Gastroenterology. 1992 Dec;103(6):1928-49
1333429
Gastroenterology. 1997 Nov;113(5):1570-5
9352859
Nature. 1988 Mar 31;332(6163):411-5
2451132
Gastroenterology. 2004 Mar;126(3):903-13
14988844
Aliment Pharmacol Ther. 2002 Feb;16(2):167-80
11860399
Scand J Gastroenterol Suppl. 1996;220:27-40
8898433
Gastroenterology. 1983 Jun;84(6):1541-6
6840484
Gastroenterology. 1994 Apr;106(4):988-93
8144004
Dig Dis Sci. 1999 May;44(5):922-6
10235598
J Cardiovasc Pharmacol. 1998;31 Suppl 1:S507-8
9595526
Gastroenterology. 1996 Mar;110(3):705-16
8608879
Eur J Pharmacol. 1989 Dec 19;174(2-3):293-6
2630303
Res Commun Mol Pathol Pharmacol. 1998 May;100(2):201-12
9667074
Helicobacter. 1996 Mar;1(1):6-19
9398908