Deleted in polyposis 1-like 1 gene (Dp1l1): a novel gene richly expressed in retinal ganglion cells.
OBJECTIVE
To characterize a novel gene, deleted in polyposis 1-like 1 (Dp1l1), which is expressed in the retina.


METHODS
A systematic screening by subtraction hybridization of the cDNAs from mouse retina and mouse brain was performed to obtain novel genes expressed in the retina. In situ hybridization, immunohistochemistry, and intracellular localization analyses were performed to investigate the expression patterns of Dp1l1. The chromosomal location of Dp1l1 was determined by radiation hybrid mapping. Bioinformatics was used for homology analysis.


RESULTS
A novel gene, Dp1l1, was expressed abundantly in the retina. It encodes a 201-amino-acid protein, and the encoded protein is designated mouse TB2-like 1. It is highly homologous to the mouse TB2, which is encoded by deleted in polyposis 1 (Dp1). In situ hybridization and immunohistochemical analyses showed that Dp1l1 mRNA and the TB2-like 1 were localized richly in retinal ganglion cells (RGCs). TB2-like 1 was present in the cytoplasm in a punctate pattern. Dp1l1 was mapped to mouse chromosome 10 by radiation hybrid mapping.


CONCLUSIONS
TB2-like 1 is a membrane protein that belongs to the YOP1/TB2/DP1/HVA22 family, and it probably plays an important role in intracellular membrane trafficking in RGCs, based on the properties of other homologous proteins.
Sato
Hajime
H
Department of Ophthalmology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan. hasato@oph.med.tohoku.ac.jp
Tomita
Hiroshi
H
Nakazawa
Toru
T
Wakana
Shigeharu
S
Tamai
Makoto
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012333:RNA, Messenger / Q000378:metabolism; D021101:Radiation Hybrid Mapping; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C499240:Deleted in polyposis 1-like 1 gene protein, mouse; D005136:Eye Proteins; D012333:RNA, Messenger; C499241:Reep6 protein, rat