title: Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture.
abstract: Tula hantavirus carrying recombinant S RNA segment (recTULV) grew in a cell culture to the same titers as the original cell adapted variant but presented no real match to the parental virus. Our data showed that the lower competitiveness of recTULV could not be increased by pre-passaging in the cell culture. Nevertheless, the recombinant virus was able to survive in the presence of the parental virus during five consecutive passages. The observed survival time seems to be sufficient for transmission of newly formed recombinant hantaviruses in nature.
authors.affiliation: Haartman Institute, Department of Virology, University of Helsinki POB 21, FIN-00014, Helsinki, Finland. anguelina.pljusnina@helsinki.fi
authors.forename: Angelina
authors.initials: A
authors.lastname: Plyusnina
authors.forename: Alexander
authors.initials: A
authors.lastname: Plyusnin
mesh_terms: D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral; D005818:Genetic Engineering; D006476:Orthohantavirus / Q000235:genetics* / Q000502:physiology*; D012367:RNA, Viral; D012692:Serial Passage; D014709:Vero Cells; D014758:Viral Core Proteins / Q000235:genetics*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus
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