[The simultaneous analysis of substance P, somatostatin, neurokinin and neurotensin by capillary zone electrophoresis].
Neuropeptides play an important part in several different areas of neurochemistry. Recently, capillary electrophoresis (CE) was developed for analysis of neuropeptides. This paper reports a capillary zone electrophoretic method for the simultaneous separation and quantitative determination of the substance P (SP), somatostatin (SS), neurokinin (NKA) and neurotensin (NT). The separation was performed on a 50microm x 60cm fused-silica capillary using 0.1mol/L at pH 2.7 phosphate as buffer. The eluted fractions were detected at 214nm.
Fu
S
S
Respiratory Disease Research Institute, Centre of Chemical Detection, China Medical University, Shenyang, 110001.
Cai
L
L
Li
Y
Y
D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015288:Neurokinin A / Q000032:analysis*; D009496:Neurotensin / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis*; D013373:Substance P / Q000032:analysis*
D013373:Substance P; D009496:Neurotensin; D013004:Somatostatin; D015288:Neurokinin A