Determination of limonoate and nomilinoate A-ring lactones in citrus juices by liquid chromatography-electrospray ionization mass spectrometry.
The development of delayed bitterness in citrus products is a major problem to citrus producers and juice processors worldwide. A rapid and sensitive liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method has been developed to quantify the recognized precursors of limonoid derived delayed bitterness, limonoate and nomilinoate A-ring lactones, in a wide variety of citrus juices. The limonoid A-ring lactones were isolated by solid-phase extraction from juice samples, analyzed by negative ion LC-ESI-MS and quantified utilizing the standard addition method.
Breksa
Andrew P
AP
Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA. apb3@pw.usda.gov
Zukas
Audrius A
AA
Manners
Gary D
GD
D001628:Beverages / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D036701:Limonins / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D036701:Limonins