[The protective effects of Fufang Biejiafang on bleomycin-induced pulmonary fibrosis in rats].
OBJECTIVE
To explore the possible effects and mechanism of Fufang Biejiafang on a single intratracheal instillation (IT) of bleomycin-induced lung fibrosis model.


METHODS
SD rats were treated with a single IT dose of bleomycin or control saline. Chinese medicine group were poured into the stomach after the first day of operation with high dosage, middle dosage and low dosage. On days 7, 14 and 28 following IT bleomycin or saline, 4 mL blood were taken from the abdominal aorta for arterial blood gas analysis. The left lung was fixed for routine light microscopic examination. Bronchoalveolar lavage fluid (BALF) from the right lung was tested the activity of pulmonary surfactant (PS) by the Whihelmy Film Balance, then the right lung was frozen immediately in liquid nitrogen for determination of hydroxyproline concentration.


RESULTS
Model rats had obviously changes of body weight and hypoxemia and dysfunction of PS on days 7 and improved on days 14. Compared with three dose groups, the middle dose group some degreely improved and PS function. It ameliorate fibrosis because of inhibition of inflammation.


CONCLUSIONS
(1) PS dysfunction resulted in hypoxemia after bleomycin injured alveolar type II (AT II). Fufang biejiafang-middle dose-group ameliorate hypoxemia by remission AT-II injury. (2) Fufang biejiafang may inhibit exudation inflammation and ameliorate fibrosis.
Kong
Lu
L
The lab of cell and biochemistry, Beijing University of Chinese Medicine, Beijing 100029, China.
Wang
Ji-feng
JF
Niu
Jian-zhao
JZ
Xia
Zhi-gang
ZG
Qiao
Chen
C
Wei
Yan-fei
YF
Tu
Heng-jing
HJ
D000818:Animals; D001761:Bleomycin; D001784:Blood Gas Analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D029596:Paeonia / Q000737:chemistry; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014426:Turtles
D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D020011:Protective Agents; D011663:Pulmonary Surfactants; D001761:Bleomycin