Efficient immobilization of enzymes on microchannel surface through His-tag and application for microreactor.
We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes.
Miyazaki
Masaya
M
Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Saga, Japan.
Kaneno
Jun
J
Yamaori
Susumu
S
Honda
Takeshi
T
Briones
Maria Portia P
MP
Uehara
Masato
M
Arima
Kazunari
K
Kanno
Kenichi
K
Yamashita
Kenichi
K
Yamaguchi
Yoshiko
Y
Nakamura
Hiroyuki
H
Yonezawa
Hiroo
H
Fujii
Masayuki
M
Maeda
Hideaki
H
D000818:Animals; D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006639:Histidine / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D013499:Surface Properties
D004800:Enzymes, Immobilized; D006639:Histidine; D007770:L-Lactate Dehydrogenase