title: Loss of heterozygosity at chromosomes 3p and 17p in primary non-small cell lung cancer.
abstract: BACKGROUND
  Loss of heterozygosity (LOH) of selected regions at chromosomes 3p and 17p in non-small cell lung cancer (NSCLC) and the association of these abnormalities with major clinical parameters and prognosis were studied.
  
  
  METHODS
  The study group included 92 consecutive primary NSCLC tumours and four microsatellite markers from chromosome 3p and three markers from 17p were analyzed.
  
  
  RESULTS
  LOH of at least one locus was found in 83% of all analyzed tumours. Most frequently deletion (58%) was found at locus D3S1481 (3p14.2). Sequence deletions of D17S520 (17p12) and TP53 (17p13.1) occurred in 52% of tumours. LOH occurrence at 3p and 17p was more frequent in squamous cell carcinomas compared to adenocarcinomas (89% vs. 75%), but this difference was not significant.
  
  
  CONCLUSIONS
  No significant association was found between LOH on any analyzed loci and tumour stage (TNM) and grade (G). There was no correlation between LOH and survival.
authors.affiliation: Department of Biology and Genetics, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chmara
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wozniak
authors.forename: Karolina
authors.initials: K
authors.lastname: Ochman
authors.forename: Grazyna
authors.initials: G
authors.lastname: Kobierska
authors.forename: Rafal
authors.initials: R
authors.lastname: Dziadziuszko
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sosinska-Mielcarek
authors.forename: Ewa
authors.initials: E
authors.lastname: Jassem
authors.forename: Jan
authors.initials: J
authors.lastname: Skokowski
authors.forename: Jacek
authors.initials: J
authors.lastname: Jassem
authors.forename: Janusz
authors.initials: J
authors.lastname: Limon
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male