title: BFRF1 of Epstein-Barr virus is essential for efficient primary viral envelopment and egress.
abstract: The molecular mechanisms that underlie maturation and egress of Epstein-Barr virus (EBV) virions are only partially characterized. We have recently shown that the BFRF1 gene, the EBV positional homolog of herpes simplex virus type 1 and pseudorabies virus UL34, is expressed early during EBV lytic replication and that it is found predominantly on the nuclear membrane (A. Farina, R. Santarelli, R. Gonnella, R. Bei, R. Muraro, G. Cardinali, S. Uccini, G. Ragona, L. Frati, A. Faggioni, and A. Angeloni, J. Virol. 74:3235-3244, 2000). These data suggest that the BFRF1 protein might be involved in viral primary envelopment. To precisely determine the function of this protein, we have constructed an EBV mutant devoid of the BFRF1 gene (BFRF1-KO). 293 cells carrying BFRF1-KO showed no differences in comparison with wild-type EBV in terms of DNA lytic replication or expression of late viral proteins upon induction of the lytic cycle. However, binding assays and infection experiments using cell lines or human cord blood lymphocytes showed a clear reduction in the viral mutant titers. Complementation experiments with BFRF1-KO and a BFRF1 expression vector restored viral titers to levels similar to those for the wild-type control, showing that the modifications that we introduced were limited to the BFRF1 gene. Electron microscopic observations showed that the reduction in viral titers was due to sequestration of EBV nucleocapsids in the nuclei of lytically induced cells. This suggests that BFRF1 is involved in transport of the maturing virion across the nuclear membrane. This hypothesis was further supported by the observation that BFRF1 is present in maturing intracellular virions but not in their extracellular counterparts. This implies that BFRF1 is a key protein for EBV maturation.
authors.affiliation: Istituto Pasteur Fondazione Cenci-Bolognetti, Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Rome, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Farina
authors.forename: Regina
authors.initials: R
authors.lastname: Feederle
authors.forename: Salvatore
authors.initials: S
authors.lastname: Raffa
authors.forename: Roberta
authors.initials: R
authors.lastname: Gonnella
authors.forename: Roberta
authors.initials: R
authors.lastname: Santarelli
authors.forename: Luigi
authors.initials: L
authors.lastname: Frati
authors.forename: Antonio
authors.initials: A
authors.lastname: Angeloni
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Torrisi
authors.forename: Alberto
authors.initials: A
authors.lastname: Faggioni
authors.forename: Henri-Jacques
authors.initials: HJ
authors.lastname: Delecluse
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology; D002478:Cells, Cultured; D004261:DNA Replication; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D005816:Genetic Complementation Test; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D014771:Virion / Q000378:metabolism; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: C406064:BFRF1 protein, Human herpesvirus 4; D008565:Membrane Proteins; D014764:Viral Proteins
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