title: A mutation in dVps28 reveals a link between a subunit of the endosomal sorting complex required for transport-I complex and the actin cytoskeleton in Drosophila.
abstract: Proteins that constitute the endosomal sorting complex required for transport (ESCRT) are necessary for the sorting of proteins into multivesicular bodies (MVBs) and the budding of several enveloped viruses, including HIV-1. The first of these complexes, ESCRT-I, consists of three proteins: Vps28p, Vps37p, and Vps23p or Tsg101 in mammals. Here, we characterize a mutation in the Drosophila homolog of vps28. The dVps28 gene is essential: homozygous mutants die at the transition from the first to second instar. Removal of maternally contributed dVps28 causes early embryonic lethality. In such embryos lacking dVps28, several processes that require the actin cytoskeleton are perturbed, including axial migration of nuclei, formation of transient furrows during cortical divisions in syncytial embryos, and the subsequent cellularization. Defects in actin cytoskeleton organization also become apparent during sperm individualization in dVps28 mutant testis. Because dVps28 mutant cells contained MVBs, these defects are unlikely to be a secondary consequence of disrupted MVB formation and suggest an interaction between the actin cytoskeleton and endosomal membranes in Drosophila embryos earlier than previously appreciated.
authors.affiliation: Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9111, USA.
authors.forename: Evgueni A
authors.initials: EA
authors.lastname: Sevrioukov
authors.forename: Nabil
authors.initials: N
authors.lastname: Moghrabi
authors.forename: Mary
authors.initials: M
authors.lastname: Kuhn
authors.forename: Helmut
authors.initials: H
authors.lastname: Krämer
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D005260:Female; D017344:Genes, Insect; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D013091:Spermatogenesis; D033921:Vesicular Transport Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000199:Actins; D029721:Drosophila Proteins; D033921:Vesicular Transport Proteins
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7626-31
references.pmid: 12802020
references.citation: Mech Dev. 1995 Apr;50(2-3):201-16
references.pmid: 7619731
references.citation: Curr Opin Cell Biol. 1998 Aug;10(4):513-22
references.pmid: 9719873
references.citation: Nat Med. 2003 Oct;9(10):1262-3
references.pmid: 14520374
references.citation: Cell. 1991 Dec 20;67(6):1145-55
references.pmid: 1760842
references.citation: Semin Cell Dev Biol. 2002 Apr;13(2):91-7
references.pmid: 12127141
references.citation: Curr Biol. 2003 Oct 14;13(20):R813-4
references.pmid: 14561424
references.citation: Mech Dev. 2000 Mar 1;91(1-2):451-4
references.pmid: 10704882
references.citation: Genetics. 1999 Feb;151(2):675-84
references.pmid: 9927460
references.citation: Development. 2002 Jan;129(2):517-26
references.pmid: 11807042
references.citation: Mol Biol Cell. 2003 Jul;14(7):2908-20
references.pmid: 12857874
references.citation: Dev Biol. 1998 Dec 1;204(1):151-64
references.pmid: 9851849
references.citation: Genetics. 1996 Dec;144(4):1673-9
references.pmid: 8978054
references.citation: J Virol. 2003 Apr;77(8):4794-804
references.pmid: 12663786
references.citation: Genome Biol. 2002;3(12):RESEARCH0080
references.pmid: 12537569
references.citation: Cell. 2001 Oct 5;107(1):55-65
references.pmid: 11595185
references.citation: Cell. 2001 Jul 27;106(2):145-55
references.pmid: 11511343
references.citation: Traffic. 2004 Mar;5(3):194-210
references.pmid: 15086794
references.citation: J Cell Biol. 2002 Nov 11;159(3):477-87
references.pmid: 12427870
references.citation: J Biol Chem. 2004 Aug 20;279(34):36059-71
references.pmid: 15218037
references.citation: Mol Biol Cell. 2002 Jul;13(7):2486-501
references.pmid: 12134085
references.citation: Methods Cell Biol. 1994;44:489-505
references.pmid: 7707968
references.citation: Curr Opin Neurol. 1997 Oct;10(5):436-42
references.pmid: 9330892
references.citation: Development. 1998 May;125(10):1833-43
references.pmid: 9550716
references.citation: Cell. 1987 Oct 9;51(1):151-8
references.pmid: 3115594
references.citation: Genetics. 1999 Sep;153(1):135-77
references.pmid: 10471706
references.citation: J Virol. 2003 Jun;77(11):6507-19
references.pmid: 12743307
references.citation: Development. 1998 Apr;125(7):1295-303
references.pmid: 9477328
references.citation: Dev Cell. 2002 Aug;3(2):271-82
references.pmid: 12194857
references.citation: Development. 2003 Aug;130(16):3725-33
references.pmid: 12835389
references.citation: Development. 2003 May;130(9):1805-16
references.pmid: 12642486
references.citation: Development. 2001 Mar;128(6):815-26
references.pmid: 11222137
references.citation: Traffic. 2001 Sep;2(9):612-21
references.pmid: 11555415
references.citation: Development. 1995 Jun;121(6):1937-46
references.pmid: 7601006
references.citation: J Cell Biol. 2003 Oct 13;163(1):143-54
references.pmid: 14530382
references.citation: Cell. 2003 Sep 19;114(6):701-13
references.pmid: 14505570
references.citation: Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1859-64
references.pmid: 11172041
references.citation: J Cell Biol. 1996 Mar;132(6):1011-23
references.pmid: 8601581
references.citation: Nat Rev Mol Cell Biol. 2004 Apr;5(4):317-23
references.pmid: 15071556
references.citation: Dev Biol. 2001 Sep 15;237(2):282-94
references.pmid: 11543614
references.citation: Mol Biol Cell. 2004 Sep;15(9):4337-46
references.pmid: 15240819
references.citation: Genetics. 1999 Aug;152(4):1631-9
references.pmid: 10430588
references.citation: J Cell Biol. 2003 Aug 4;162(3):435-42
references.pmid: 12900395
references.citation: Dev Cell. 2002 Aug;3(2):283-9
references.pmid: 12194858
references.citation: Nat Cell Biol. 1999 May;1(1):72-4
references.pmid: 10559868
references.citation: Mol Biol Cell. 2004 Jan;15(1):345-58
references.pmid: 14617814
references.citation: Development. 1993 Apr;117(4):1223-37
references.pmid: 8404527
references.citation: Mol Biol Cell. 2000 Mar;11(3):1011-22
references.pmid: 10712516
references.citation: J Cell Biol. 2000 May 15;149(4):901-14
references.pmid: 10811830
references.citation: J Cell Biol. 1996 Jul;134(1):133-48
references.pmid: 8698810
references.citation: Annu Rev Cell Dev Biol. 2003;19:287-332
references.pmid: 14570572
references.citation: Development. 1991 Apr;111(4):909-20
references.pmid: 1879360
references.citation: J Cell Biol. 1996 Aug;134(4):923-34
references.pmid: 8769417
references.citation: J Biol Chem. 2001 Apr 13;276(15):11735-42
references.pmid: 11134028
references.citation: J Cell Sci. 1999 Sep;112 ( Pt 17):2885-93
references.pmid: 10444383
references.citation: J Cell Biol. 1994 Dec;127(6 Pt 1):1637-53
references.pmid: 7798318
references.citation: Nat Rev Mol Cell Biol. 2002 Dec;3(12):893-905
references.pmid: 12461556
references.citation: J Cell Biol. 2002 Aug 19;158(4):669-79
references.pmid: 12177043
references.citation: Curr Biol. 2003 Oct 28;13(21):1848-57
references.pmid: 14588240
references.citation: Nucleic Acids Res. 1988 Feb 11;16(3):1043-61
references.pmid: 3125519
references.citation: EMBO J. 1993 Jul;12(7):2855-62
references.pmid: 8335001
references.citation: Mol Cell Biol. 2003 Jan;23(1):150-62
references.pmid: 12482969
references.citation: J Cell Biol. 2003 Aug 4;162(3):425-34
references.pmid: 12900394
references.citation: Nature. 1991 Jul 18;352(6332):207-12
references.pmid: 1857416
references.citation: Mol Biol Cell. 1996 Jun;7(6):985-99
references.pmid: 8817003
references.citation: Cell. 1997 Oct 3;91(1):109-18
references.pmid: 9335339
references.citation: Genes Dev. 2002 Jun 1;16(11):1314-36
references.pmid: 12050111
references.citation: Mol Biol Cell. 1998 Apr;9(4):809-16
references.pmid: 9529379
references.citation: Dev Cell. 2003 Jul;5(1):139-48
references.pmid: 12852858
references.citation: Mol Cell. 1999 Oct;4(4):479-86
references.pmid: 10549280
references.citation: Curr Opin Cell Biol. 2003 Aug;15(4):446-55
references.pmid: 12892785
references.citation: Mol Biol Cell. 2004 Feb;15(2):838-50
references.pmid: 14657248
references.citation: Cell. 2003 Nov 14;115(4):475-87
references.pmid: 14622601
references.citation: J Cell Sci. 2002 Dec 15;115(Pt 24):4855-65
references.pmid: 12432073
references.citation: Genetics. 1988 Mar;118(3):461-70
references.pmid: 2835286
references.citation: J Cell Biol. 2003 Aug 4;162(3):413-23
references.pmid: 12900393
references.citation: Science. 2004 Jan 23;303(5657):531-4
references.pmid: 14739459
references.citation: Nature. 1996 Oct 17;383(6601):627-30
references.pmid: 8857539
references.citation: Cell. 2002 Jan 25;108(2):261-9
references.pmid: 11832215
references.citation: Development. 1993 Jun;118(2):401-15
references.pmid: 8223268
references.citation: Traffic. 2002 Feb;3(2):87-91
references.pmid: 11929598
references.citation: J Cell Biol. 2002 Apr 1;157(1):91-101
references.pmid: 11916981
references.citation: Science. 2000 Mar 24;287(5461):2185-95
references.pmid: 10731132
references.citation: Genes Dev. 2000 Jul 1;14(13):1651-63
references.pmid: 10887159
references.citation: Mech Dev. 1991 Jun;34(2-3):101-11
references.pmid: 1911395
references.citation: Mol Biol Cell. 1993 Sep;4(9):885-96
references.pmid: 8257792
references.citation: Mol Gen Genet. 2000 Jul;263(6):1003-14
references.pmid: 10954086
references.citation: Mol Biol Cell. 2001 Nov;12(11):3451-64
references.pmid: 11694580
references.citation: EMBO J. 1997 Aug 1;16(15):4508-18
references.pmid: 9303295
references.citation: Curr Biol. 2002 Jan 22;12(2):R74-8
references.pmid: 11818085
references.citation: Traffic. 2000 Mar;1(3):248-58
references.pmid: 11208108
references.citation: Dev Biol. 1999 Jan 1;205(1):129-44
references.pmid: 9882502
references.citation: J Biol Chem. 2002 Nov 8;277(45):43216-23
references.pmid: 12205095
references.citation: J Cell Biol. 1996 Jun;133(6):1205-15
references.pmid: 8682859
references.citation: Trends Cell Biol. 1998 Jul;8(7):257-9
references.pmid: 9714595
references.citation: Mol Biol Cell. 1999 Apr;10(4):847-59
references.pmid: 10198042
references.citation: Cell. 1998 Dec 11;95(6):847-58
references.pmid: 9865702