title: Hyaluronan synthase expression in pleural malignant mesotheliomas.
abstract: Hyaluronan (HA) is thought to play several important roles in tumor growth, tumorigenicity, and tumor dissemination and metastasis. Recently, three isoforms of hyaluronan synthase (HAS) have been cloned. Our objective was to determine which of the HAS isoforms were expressed in pleural malignant mesotheliomas, the most representative lesion of HA-producing tumors. We studied 10 cases of pleural malignant mesothelioma using novel antibodies of HAS. We compared HAS expression patterns of mesothelioma and pulmonary adenocarcinoma. Immunohistochemically, 9 of 10 (90%) cases of mesothelioma had extensive reaction to anti-HAS1 and anti-HAS2 antibodies, while HAS3 overexpression was present in 4 of 10 cases (40%). Of 20 cases of pulmonary adenocarcinoma, 5 overexpressed HAS1 (25%), 16 of 20 HAS2 (80%), and 4 of 20 HAS3 (20%). The expression level of HAS1 was significantly higher in mesotheliomas than in pulmonary adenocarcinoma (P=0.0036). Our data suggests that HAS1 might be a useful positive marker of malignant mesothelioma. However, a definitive conclusion should be based on further large-scale studies.
authors.affiliation: Pathology Division, National Cancer Center Research Institute East, 6-5-1 Kashiwanoha, 277-8577 Kashiwa, Chiba, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanomata
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yokose
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kamijo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yonou
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hasebe
authors.forename: Naoki
authors.initials: N
authors.lastname: Itano
authors.forename: Koji
authors.initials: K
authors.lastname: Kimata
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ochiai
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D005260:Female; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D006801:Humans; D000076002:Hyaluronan Synthases; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D008654:Mesothelioma / Q000201:enzymology*; D008875:Middle Aged; D010997:Pleural Neoplasms / Q000201:enzymology*
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases
references.citation: Br J Exp Pathol. 1963 Feb;44:81-3
references.pmid: 13952822
references.citation: J Invest Dermatol. 1999 Dec;113(6):935-9
references.pmid: 10594733
references.citation: Cancer Res. 1999 May 15;59(10):2499-504
references.pmid: 10344764
references.citation: Am J Pathol. 2000 Jun;156(6):2159-67
references.pmid: 10854236
references.citation: Am J Clin Pathol. 1998 Sep;110(3):397-404
references.pmid: 9728617
references.citation: Cancer Res. 1993 Jan 15;53(2):388-92
references.pmid: 8417831
references.citation: Clin Chem. 1994 May;40(5):777-80
references.pmid: 8174251
references.citation: J Biol Chem. 1996 Sep 20;271(38):22945-8
references.pmid: 8798477
references.citation: Am J Pathol. 1996 Jun;148(6):1721-6
references.pmid: 8669457
references.citation: J Biol Chem. 1996 Sep 20;271(38):23395-9
references.pmid: 8798544
references.citation: Chest. 1988 Nov;94(5):1037-9
references.pmid: 3180855
references.citation: Biochem Biophys Res Commun. 1996 May 24;222(3):816-20
references.pmid: 8651928
references.citation: Cancer Res. 1983 Mar;43(3):1347-54
references.pmid: 6825105
references.citation: Cancer. 1979 Jul;44(1):221-7
references.pmid: 455246
references.citation: Clin Exp Metastasis. 2004;21(1):57-63
references.pmid: 15065603
references.citation: Am J Clin Pathol. 1969 Sep;52(3):335-9
references.pmid: 4241077
references.citation: Am J Surg Pathol. 2000 Sep;24(9):1183-200
references.pmid: 10976692
references.citation: Br J Dermatol. 2001 Jul;145(1):141-5
references.pmid: 11453924
references.citation: Exp Cell Res. 2000 Jul 10;258(1):12-22
references.pmid: 10912783
references.citation: Cancer. 1994 Mar 1;73(5):1406-10
references.pmid: 8111707
references.citation: Ann Clin Lab Sci. 1973 May-Jun;3(3):207-11
references.pmid: 4122202
references.citation: Cancer Res. 2001 Jul 1;61(13):5207-14
references.pmid: 11431361
references.citation: Am J Surg Pathol. 1992 Feb;16(2):116-21
references.pmid: 1733346
references.citation: Cancer Res. 1985 Apr;45(4):1850-4
references.pmid: 3978644
references.citation: Cancer Res. 1999 Mar 1;59(5):1141-5
references.pmid: 10070975
references.citation: Br J Cancer. 2001 Aug 17;85(4):600-7
references.pmid: 11506502
references.citation: Cancer Res. 1995 Jan 15;55(2):428-33
references.pmid: 7529138
references.citation: Cancer Res. 1985 Oct;45(10):5098-105
references.pmid: 4027988
references.citation: Am J Med Technol. 1982 Nov;48(11):905-8
references.pmid: 6184991