[Detection of major gene on litter size in sheep].
The current study was designed to detect SNPs within BMP15 and BMPR-IB gene and investigate the effect of the genes on sheep litter size. Four sheep lines, HU-Yang, Chinese Merino monotocous, Chinese Merino multiparous for wool production and Chinese Merino multiparous for mutton production, were used in this study. Litter sizes were recorded for each ewe in the four lines. Primers for BMP15 and BMPR-IB gene were designed from database sheep sequence and polymorphisms were detected by PCR-RFLP method. The results showed that there was no polymorphism with BMP15 gene among the four lines, and there was an A / G SNP with BMPR-IB gene at base 746 among the four lines. Three types of genotype (BB, B+ and ++), based on A / G locus, were found within each line. The frequencies of genotypes were significantly different among the lines (P<0.001), with BB genotype primarily existing in HU-Yang, ++ genotype in Chinese Merino monotocous line, and B+ genotype in Chinnese Merino multiparous lines. The A / G mutation influence significantly the sheep litter sizes, and the BB and B+ ewes had significant higher litter sizes than ++ ewes. The results of present study showed simultaneously that the genotype of BMPR-IB was a perfect predictor of the sheep litter sizes. These results intensively indicated that BMPR-IB is a major gene to affect litter size in sheep, and could be used as the molecular genetic marker to select litter size in sheep.
Wang
Qi-Gui
QG
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. wangqigui@hotmail.com
Zhong
Fa-Gang
FG
Li
Hui
H
Wamg
Xin-Hua
XH
Liu
Shou-Ren
SR
Chen
Xiao-Jun
XJ
Gan
Shang-Quan
SQ
D000818:Animals; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics*; D002681:China; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D055429:Growth Differentiation Factor 9; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008098:Litter Size / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012756:Sheep / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
D005819:Genetic Markers; D055429:Growth Differentiation Factor 9; D036341:Intercellular Signaling Peptides and Proteins; D052005:Bone Morphogenetic Protein Receptors, Type I