title: Inflammatory cell infiltration and high antibody production in BALB/c mice caused by natural exposure to Lutzomyia longipalpis bites.
abstract: Sand flies inject saliva into the mammalian host when probing for a blood meal. Understanding the initial vertebrate reactions against sand fly saliva is important for possible interventions because these insects transmit diseases to humans and other animals. Little is known of these reactions to New World sand flies. Repeated exposure of BALB/c mice to Lutzomyia longipalpis bites leads to local inflammatory cell infiltration comprised of neutrophils, macrophages, and eosinophils. Total IgG and IgG1 antibodies react predominantly with three major protein bands (45, 44, and 16 kD) of the insect saliva by Western blot. The injection of immune serum previously incubated with salivary gland homogenate induced an early infiltration with neutrophils and macrophages, suggesting the participation of immune complexes in triggering inflammation.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Salvador, Bahia, Brazil.
authors.forename: Francinaldo
authors.initials: F
authors.lastname: Silva
authors.forename: Regis
authors.initials: R
authors.lastname: Gomes
authors.forename: Deboraci
authors.initials: D
authors.lastname: Prates
authors.forename: José C
authors.initials: JC
authors.lastname: Miranda
authors.forename: Bruno
authors.initials: B
authors.lastname: Andrade
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
authors.forename: Aldina
authors.initials: A
authors.lastname: Barral
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D007299:Insect Bites and Stings / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011576:Psychodidae / Q000276:immunology*; D012463:Saliva / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000906:Antibodies