title: Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice.
abstract: The cloning of hevein genes from Hevea brasiliensis was undertaken with the objective to isolate useful promoters to drive transgene expression in genetically engineered rubber tree. Four different full length genes were cloned by library screening and a fifth, a partial gene, by adaptor-anchored PCR. Sequence alignment revealed that hevein genes, although highly conserved in their transcribed region, diverged in two groups, with major differences in their promoter region, suggesting a more rapid evolution of the upstream regulatory functions of the genes than the downstream functions of their protein products. The promoter regions from two hevein genes representative of each group were isolated and analyzed in rice. Although both were functional, only the longest promoter sequence (PHev2.1) conferred a high level of expression to the transgene in various tissues of this heterologous host. It was in addition up-regulated by mechanical wounding and fungal infection in leaves. A number of potential cis-regulatory elements were identified in silico and are discussed in view of the expression profiles observed in rice.
authors.affiliation: CIRAD, TA 80/03, Avenue Agropolis, 34398 Montpellier cedex, France. valerie.pujade-renaud@cirad.fr <valerie.pujade-renaud@cirad.fr>
authors.forename: Valérie
authors.initials: V
authors.lastname: Pujade-Renaud
authors.forename: Christine
authors.initials: C
authors.lastname: Sanier
authors.forename: Laurence
authors.initials: L
authors.lastname: Cambillau
authors.forename: Arokiaraj
authors.initials: A
authors.lastname: Pappusamy
authors.forename: Heddwyn
authors.initials: H
authors.lastname: Jones
authors.forename: Natsuang
authors.initials: N
authors.lastname: Ruengsri
authors.forename: Didier
authors.initials: D
authors.lastname: Tharreau
authors.forename: Hervé
authors.initials: H
authors.lastname: Chrestin
authors.forename: Pascal
authors.initials: P
authors.lastname: Montoro
authors.forename: Jarunya
authors.initials: J
authors.lastname: Narangajavana
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005966:Glucuronidase / Q000235:genetics; D028482:Hevea / Q000235:genetics*; D005810:Multigene Family; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D005966:Glucuronidase