title: Removal of contaminating DNA from commercial nucleic acid extraction kit reagents.
abstract: Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained.
authors.affiliation: Sanquin Blood Bank North West Region, Amsterdam, The Netherlands.
authors.forename: Tamimount
authors.initials: T
authors.lastname: Mohammadi
authors.forename: Henk W
authors.initials: HW
authors.lastname: Reesink
authors.forename: Christina M J E
authors.initials: CM
authors.lastname: Vandenbroucke-Grauls
authors.forename: Paul H M
authors.initials: PH
authors.lastname: Savelkoul
mesh_terms: D001792:Blood Platelets / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005189:False Positive Reactions; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D011933:Reagent Kits, Diagnostic / Q000592:standards*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic