title: Overexpression of cyclin D1 and interaction between p27Kip1 and tumour thickness predict lymph node metastases occurrence in lower lip squamous cell carcinoma.
abstract: We have attempted to identify those subgroups of patients most likely to develop lymph node metastases from squamous cell carcinoma of the lower lip (LLSSC). A total of 97 subjects, who did not undergo elective neck dissection, were recruited into the 60-month disease-free survival study. After univariate analysis, tumour size, histological grading, maximal thickness, perineural invasion and immunoreactivity to cyclin D1 and p27Kip1 proteins proved to be significant factors. Tests of the effect of interaction between p27Kip1 LI and tumour thickness yielded that the impact of tumour thickness on the risk of lymph node metastases was modified by the percentage of p27Kip1 positive cells. Subsequent to models of multivariate analysis, tumour size, positive cyclin D1 protein expression, maximal thickness (> 5 mm), p27Kip1 LI (%) and the interaction term between p27Kip1 LI and tumour thickness retained strong independent predictive values for lymph node metastases. We suggest that immunohistochemistry for cyclin D1 and p27Kip1 may prove to be valuable ancillary tests for identifying LLSSC with metastatic potential.
authors.affiliation: Institute of Pathological Anatomy and Histology, University of Palermo, Via del Vespro 129, 90127 Palermo, Italy. vitorodolico@virgilio.it
authors.forename: Vito
authors.initials: V
authors.lastname: Rodolico
authors.forename: Federico
authors.initials: F
authors.lastname: Aragona
authors.forename: Daniela
authors.initials: D
authors.lastname: Cabibi
authors.forename: Cristofaro
authors.initials: C
authors.lastname: Di Bernardo
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Lorenzo
authors.forename: Nicola
authors.initials: N
authors.lastname: Gebbia
authors.forename: Gaspare
authors.initials: G
authors.lastname: Gulotta
authors.forename: Vincenza
authors.initials: V
authors.lastname: Leonardi
authors.forename: Francesca
authors.initials: F
authors.lastname: Ajello
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000032:analysis*; D019938:Cyclin D1 / Q000032:analysis*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008048:Lip Neoplasms / Q000737:chemistry* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012044:Regression Analysis; D025521:Tumor Suppressor Proteins / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D025521:Tumor Suppressor Proteins; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27