title: Phylogenetic analyses of human rotavirus in central Taiwan in 1996, 2001 and 2002.
abstract: BACKGROUND
  Rotavirus epidemiology information is required for gastroenteritis disease control and prevention. Information gathered about the serotype distribution of rotaviruses isolated in Taiwan is of crucial significance, before a licensed rotavirus vaccine is introduced.
  
  
  OBJECTIVE
  The purpose of the present study is to investigate the epidemiological diversity of rotaviruses in Taiwan.
  
  
  METHODS
  A total of 51 stool samples taken from cases of acute gastroenteritis were collected from three teaching hospitals in central Taiwan in 1996, 2001 and 2002. The samples were subjected to RT-PCR tests of VP7 gene of the human rotavirus group A, B, C.
  
  
  RESULTS
  A total of 16 stool samples were detected positive by RT-PCR and 10 were sequence analyzed and classified into G1, G3, and G9 types. Compared with other HRV strains: the sequences of CS96-40 of G1 are similar to MVD9816 (identity rate 97.15% and 96.09%, respectively, from Uruguay); the sequences of CS02-01 of G3 are similar to 98-B31 (identity rate 98.93% and 98.72%, respectively, from Japan); the sequences of CS01-05, CS01-06, CS01-07, CS01-09, CS01-13, CS02-02, CS02-03, CS02-04 are very similar to other established G9 rotaviruses sequences (identity rate 96.85-99.88%), especially between CS02-04 and SP2737 (from Japan) with an identity rate of 99.88% and 100% nucleotide and amino acid, respectively. Except for CS01-06 strain, it is VR3, but not VR5, VR7 or VR8, that found to be the most frequent mutated amino acid regions of VP7 in these strains.
  
  
  CONCLUSIONS
  Our findings are the first to document the high prevalence of G9 HRV strains in Taiwan, and suggest the re-emergence of G3 strains in central Taiwan since 1991. Epidemiological surveys carried out in this study suggest genotype shifts from type G1 before 1996, to G9 in 2001 and 2002 and the re-emergence of G3 type in 2002.
authors.affiliation: Department of Pediatric, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC.
authors.forename: Hsin-Chuan
authors.initials: HC
authors.lastname: Lai
authors.forename: Shyh-Jye
authors.initials: SJ
authors.lastname: Lin
authors.forename: Hui-Ru
authors.initials: HR
authors.lastname: Lin
authors.forename: Chin-Shein
authors.initials: CS
authors.lastname: Ku
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.forename: Chi-Chiang
authors.initials: CC
authors.lastname: Yang
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004279:DNA, Viral; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013624:Taiwan / Q000453:epidemiology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus