title: Immune activation modulates hematopoiesis through interactions between CD27 and CD70.
abstract: The differentiation of hematopoietic stem cells into mature blood cell lineages is tightly regulated. Here we report that CD27, which is expressed on stem and early progenitor cells in bone marrow, can be important in this process. Deletion of CD27 increased the myeloid colony-forming potential of stem and early progenitor cells and enhanced B lymphoid reconstitutive capacity in competitive transplantation experiments. Conversely, stimulation of CD27(+) progenitor cells with CD70, the unique ligand for CD27, inhibited colony-forming potential in vitro and lymphocyte outgrowth in vivo. As CD70 is expressed only on activated immune cells, we suggest that CD27 triggering on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation.
authors.affiliation: Department of Hematology, F4-224, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands.
authors.forename: Martijn A
authors.initials: MA
authors.lastname: Nolte
authors.forename: Ramon
authors.initials: R
authors.lastname: Arens
authors.forename: Ronald
authors.initials: R
authors.lastname: van Os
authors.forename: Michiel
authors.initials: M
authors.lastname: van Oosterwijk
authors.forename: Berend
authors.initials: B
authors.lastname: Hooibrink
authors.forename: René A W
authors.initials: RA
authors.lastname: van Lier
authors.forename: Marinus H J
authors.initials: MH
authors.lastname: van Oers
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology; D053282:CD27 Ligand; D002454:Cell Differentiation / Q000276:immunology; D005434:Flow Cytometry; D006410:Hematopoiesis / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013047:Specific Pathogen-Free Organisms; D013234:Stem Cells / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D053282:CD27 Ligand; C505772:Cd70 protein, mouse; D008565:Membrane Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7