[Optimization of purification conditions for with macroporous adsorption resin total saponins from smilax china].
OBJECTIVE
To develop a with high efficiency and practicality for separating and purifying total steroidal saponins lax china.


METHODS
Using adsorption capacity and desorption rate of total steroidal saponins as the primary screening index, surveyed, and the optimized conditions of adsorption and desorption of total steroidal saponins were studied.


RESULTS
The adsorption and desorption rate of total steroidal saponins reached 16 mg x mL(-1) and 90% respectively for macroporous resin HPD100 chosen. Macroporous resin HPD100 could be well used in separating and purifying total steroidal saponins from S. china.
Shu
Xiao-Shun
XS
Institute of Materia Medica, Huazhong University of Science and Technology, Wuhan 430074, China. sxs732@sohu.com
Gao
Zhong-Hong
ZH
Yang
Xiang-Liang
XL
D000327:Adsorption; D000837:Anion Exchange Resins; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification*; D032281:Smilax / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000837:Anion Exchange Resins; D012503:Saponins