title: Osteoclast-like giant cell tumor in mucinous cystadenocarcinoma of the pancreas: an immunohistochemical and molecular analysis.
abstract: Osteoclast-like giant cell tumors (OLGT) are rare neoplasms of the pancreas and mostly associated with ductal adenocarcinomas. In this report, we present the rare case of OLGT associated with mucinous cystadenocarcinoma (MCC). We investigated the expression profile of both tumors by methods of molecular biology and immunohistochemistry. The panel of markers included osteopontin, her2/neu, mismatch repair genes, K-ras, p53, E-cadherin, VEGF-C, and podoplanin. Osteopontin was expressed by the osteoclast-like giant cells but not by the mononuclear tumor cells of the OLGT. We detected an amplification and overexpression of her2/neu in the MCC but not in the OLGT. Although we observed an immunohistochemical expression of hMSH2 and hMLH1 in the OLGT, we were not able to confirm this result by western blot analysis. We also did not find any microsatellite instability (D2S123, BAT26). While mutation of K-ras codon 12 was found in both tumor components, there was wild-type DNA of p53. E-cadherin was expressed in MCC but not in OLGT. VEGF-C was only positive in osteoclast-like giant cells and some of the mononuclear cells of OLGT. The vessel-rich stroma of OLGT did not present any podoplanin-positive lymphatic vessel. The observation of our case and others in the published literature may indicate separating OLGT with undifferentiated carcinoma from OLGT with MCC for the better clinical outcome of the latter.
authors.affiliation: Department of Pathology, Medical University Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria, Europe. roland.sedivy@meduniwien.ac.at
authors.forename: Roland
authors.initials: R
authors.lastname: Sedivy
authors.forename: Maria
authors.initials: M
authors.lastname: Kalipciyan
authors.forename: Peter R
authors.initials: PR
authors.lastname: Mazal
authors.forename: Brigitte
authors.initials: B
authors.lastname: Wolf
authors.forename: F
authors.initials: F
authors.lastname: Wrba
authors.forename: Judith
authors.initials: J
authors.lastname: Karner-Hanusch
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Mühlbacher
authors.forename: Robert M
authors.initials: RM
authors.lastname: Mader
mesh_terms: D000328:Adult; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000096:biosynthesis; D002454:Cell Differentiation; D018282:Cystadenocarcinoma, Mucinous / Q000276:immunology* / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D020869:Gene Expression Profiling; D005870:Giant Cell Tumors / Q000276:immunology* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D010010:Osteoclasts; D010190:Pancreatic Neoplasms / Q000276:immunology* / Q000473:pathology*; D016133:Polymerase Chain Reaction
chemical_list: D000912:Antibodies, Neoplasm