Development of a monoclonal blocking ELISA for the detection of antibody to Mycoplasma bovis in dairy cattle and comparison to detection by PCR.
A monoclonal antibody blocking enzyme-linked immunosorbent assay (B-ELISA) was developed to detect antibodies to Mycoplasma bovis in cattle sera. The assay was highly specific and sensitive and there was no cross-reaction detected. This method revealed a high prevalence of antibodies (60%) to M. bovis in dairy cattle in North Queensland. The diagnostic potential of this B-ELISA for the detection of antibody to M. bovis was compared with its detection by PCR. There was a strong positive correlation between PCR and B-ELISA titers. Thus, the B-ELISA appears to be a valuable and reproducible tool in the serodiagnosis of M. bovis infection in cattle.
Ghadersohi
Ali
A
Department Microbiology and Immunology and Australian Institute of Tropical Veterinary and Animal Science James Cook University, Townsville, Qld. 4811, Australia. ali-ghadersohi@yahoo.com
Fayazi
Zahra
Z
Hirst
Robert G
RG
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000382:microbiology; D009175:Mycoplasma Infections / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000276:immunology / Q000302:isolation & purification*; D011793:Queensland; D011817:Rabbits; D015203:Reproducibility of Results; D012140:Respiratory Tract Diseases / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial