Narrow-bore high performance liquid chromatographic method for the determination of cetirizine in human plasma using column switching.
An improved column switching high performance liquid chromatographic (HPLC) method was developed for determination of cetirizine in human plasma. Plasma samples were prepared by liquid-liquid extraction using methylene chloride. The samples extracted were initially injected into a clean-up Capcell Pak MF C8 column and the peaks of cetirizine and internal standard were separated to an analytical C18 micro-column via column switching device. This analysis showed highly sensitive and selective results. Also, it was successfully applied to evaluate the pharmacokinetics of cetirizine in human volunteers after single oral administration.
Kim
Chong-Kook
CK
Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong Kwanak-gu, Seoul 151-742, South Korea. ckkim@plaza.snu.ac.kr
Yeon
Kyu Jeong
KJ
Ban
Eunmi
E
Hyun
Myung-Ja
MJ
Kim
Jin-Ki
JK
Kim
Mee-Kyung
MK
Jin
Su-Eon
SE
Park
Jeong-Sook
JS
D017332:Cetirizine / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans
D017332:Cetirizine