Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling.
The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (T(H)1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse T(H)1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.
Gutjahr
Claudia
C
Max Planck Institute of Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany.
Murphy
Derek
D
Lueking
Angelika
A
Koenig
Andrea
A
Janitz
Michal
M
O'Brien
John
J
Korn
Bernhard
B
Horn
Sabine
S
Lehrach
Hans
H
Cahill
Dolores J
DJ
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling; D051379:Mice; D008822:Mice, Transgenic; D018417:Th1 Cells / Q000276:immunology*
D000906:Antibodies; D017931:DNA Primers; D018076:DNA, Complementary