Membrane topology of the Escherichia coli AmpG permease required for recycling of cell wall anhydromuropeptides and AmpC beta-lactamase induction.
Escherichia coli, and presumably most other gram-negative bacteria, possesses an efficient protein machinery for recycling its peptidoglycan during cell growth. The major recycled peptidoglycan product is N-acetylglucosamine-1,6-anhydro-N-acetylmuramic acid-tetrapeptide. Its uptake from the periplasm into the cytoplasm is carried out via the AmpG protein, an intrinsic membrane protein. In gram-negative bacteria carrying an ampC beta-lactamase-inducible gene on their chromosomes, the induction mechanism is directly linked to peptidoglycan recycling. After identification of the different putative hydrophobic segments by computing, the AmpG topology was experimentally determined by using beta-lactamase fusion. In the proposed model, AmpG contains 10 transmembrane segments and two large cytoplasmic loops.
Chahboune
Aicha
A
Départment de Biologie, Faculté des Sciences, Université My Ismail, Meknès, Morocco.
Decaffmeyer
Marc
M
Brasseur
Robert
R
Joris
Bernard
B
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D002473:Cell Wall / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000378:metabolism*; D001618:beta-Lactamases / Q000096:biosynthesis
C084284:AmpG protein, Bacteria; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D010457:Peptidoglycan; C048795:AmpC beta-lactamases; D001618:beta-Lactamases
J Bacteriol. 1970 Jan;101(1):218-31
4983650
EMBO J. 1999 Aug 2;18(15):4108-17
10428950
Annu Rev Biochem. 1984;53:595-623
6383201
J Mol Biol. 1984 Oct 15;179(1):125-42
6502707
J Bacteriol. 1985 Apr;162(1):391-7
2858468
Science. 2001 Sep 7;293(5536):1793-800
11546864
Science. 2002 May 10;296(5570):1091-8
12004122
J Bacteriol. 2002 Dec;184(23):6434-6
12426329
J Bacteriol. 1985 Jul;163(1):305-10
3891732
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4620-4
2991883
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6602-6
3413113
Antimicrob Agents Chemother. 1989 Nov;33(11):1946-51
2692514
Gene. 1990 Nov 30;96(1):51-7
2265759
Mol Microbiol. 1990 Oct;4(10):1637-44
2077355
J Gen Microbiol. 1991 Mar;137(3):441-54
1674525
J Mol Biol. 1992 May 20;225(2):487-94
1593632
Mol Microbiol. 1993 Aug;9(4):703-15
8231804
EMBO J. 1994 Oct 3;13(19):4684-94
7925310
Mol Microbiol. 1995 Aug;17(3):421-6
8559061
Cell. 1997 Mar 21;88(6):823-32
9118225
Antimicrob Agents Chemother. 1997 Oct;41(10):2113-20
9333034
Microbiol Mol Biol Rev. 1998 Mar;62(1):1-34
9529885
Microbiol Mol Biol Rev. 1998 Mar;62(1):110-29
9529889
Antimicrob Agents Chemother. 1972 Apr;1(4):283-8
4208895