title: Transient confinement zones: a type of lipid raft?
abstract: Many important signaling events are initiated at the cell membrane. To facilitate efficient signal transduction upon stimulation, membrane microdomains, also known as lipid rafts, are postulated to serve as platforms to recruit components involved in the signaling complex, but few methods exist to study rafts in vivo. Single particle tracking provides an approach to study the plasma membrane of living cells on the nano-scale. The trajectories of single gold particles bound to membrane proteins and lipids are characterized in terms of both random and confined diffusion; the latter occurs in "transient confinement zones". Here we review transient confinement zones and some of their implications for membrane structure and function.
authors.affiliation: Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7090, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.forename: Ken
authors.initials: K
authors.lastname: Jacobson
mesh_terms: D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism*; D015398:Signal Transduction; D018800:Thy-1 Antigens / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D018800:Thy-1 Antigens
references.citation: Proc Natl Acad Sci U S A. 2003 May 13;100(10):5795-800
references.pmid: 12721375
references.citation: Biophys J. 2003 Jan;84(1):356-66
references.pmid: 12524289
references.citation: Mol Biol Cell. 2003 Nov;14(11):4427-36
references.pmid: 14551254
references.citation: Biophys J. 2002 Nov;83(5):2693-701
references.pmid: 12414701
references.citation: Biochim Biophys Acta. 2003 Mar 10;1610(2):231-43
references.pmid: 12648777
references.citation: Science. 1972 Feb 18;175(4023):720-31
references.pmid: 4333397
references.citation: Annu Rev Cell Dev Biol. 1998;14:111-36
references.pmid: 9891780
references.citation: Biophys J. 1999 Nov;77(5):2638-42
references.pmid: 10545363
references.citation: Biophys J. 1998 Jan;74(1):297-308
references.pmid: 9449330
references.citation: Cell. 1992 Feb 7;68(3):533-44
references.pmid: 1531449
references.citation: Mol Membr Biol. 2003 Jan-Mar;20(1):13-8
references.pmid: 12745919
references.citation: Science. 2000 Dec 1;290(5497):1721-6
references.pmid: 11099405
references.citation: Cell. 2004 Feb 20;116(4):577-89
references.pmid: 14980224
references.citation: Science. 1995 Jun 9;268(5216):1441-2
references.pmid: 7770769
references.citation: Science. 2002 May 3;296(5569):913-6
references.pmid: 11988576
references.citation: Nature. 1997 Jun 5;387(6633):569-72
references.pmid: 9177342
references.citation: Traffic. 2004 Apr;5(4):213-30
references.pmid: 15030563
references.citation: Biophys J. 2004 Jun;86(6):4075-93
references.pmid: 15189902
references.citation: J Cell Biol. 1993 Jan;120(1):25-35
references.pmid: 8416991
references.citation: Trends Cell Biol. 1999 Mar;9(3):87-91
references.pmid: 10201072
references.citation: Nat Rev Mol Cell Biol. 2000 Oct;1(1):31-9
references.pmid: 11413487
references.citation: Biophys J. 1993 Jun;64(6):1766-80
references.pmid: 8369407
references.citation: Biol Chem. 2003 Sep;384(9):1259-63
references.pmid: 14515986
references.citation: Biochem Soc Trans. 2003 Oct;31(Pt 5):1001-5
references.pmid: 14505468
references.citation: Biophys J. 1995 Sep;69(3):989-93
references.pmid: 8519998
references.citation: Nat Neurosci. 2001 Mar;4(3):253-60
references.pmid: 11224541
references.citation: Biochemistry. 1997 Oct 14;36(41):12449-58
references.pmid: 9376349
references.citation: Annu Rev Physiol. 1987;49:163-75
references.pmid: 3551795
references.citation: Biochem Biophys Res Commun. 1997 Nov 7;240(1):1-7
references.pmid: 9367871
references.citation: Biophys J. 2002 Jan;82(1 Pt 1):274-84
references.pmid: 11751315
references.citation: J Cell Biol. 2002 Jun 10;157(6):1071-81
references.pmid: 12058021