title: Biological properties of the intervertebral cages made of titanium containing a carbon-carbon composite covered with different polymers.
abstract: Intervertebral cages are used in orthopaedics for stabilization of injured lumbar parts of vertebral columns. Our study provides preliminary results of tests of the biological properties of titanium cages with a variously modified carbon/carbon composite (C/C) core. This core was produced from a C/C composite modified by hydrogel materials based on poly(2-hydroxyethyl methacrylate) (HEMA) enriched with 1% collagen or 35% methylmethacrylate or 30% terc-butylmethacrylamide. We evaluated the adhesion of the cells to the tested material coating using an in vitro study of the metabolic activity and cytokine production of the cells (TNF-alpha, IL-8). We studied the biocompatibility of intervertebral cages coated with different copolymers under in vivo condition and in an implantation experiment in the porcine femurs. Both in vitro and in vivo results revealed favourable biotolerance of the use system. Modification of the composite HEMA with the use of collagen seems to have a more positive effect on the new bone tissue formed around the implanted devices than HEMA copolymerized with methylmethacrylate or terc-butylmethacrylamide.
authors.affiliation: Rheumatological Institute Na Slupi 4, Prague 2, 128 50, Czech Republic. pesakova@seznam.cz
authors.forename: V
authors.initials: V
authors.lastname: Pesáková
authors.forename: K
authors.initials: K
authors.lastname: Smetana
authors.forename: M
authors.initials: M
authors.lastname: Sochor
authors.forename: H
authors.initials: H
authors.lastname: Hulejová
authors.forename: K
authors.initials: K
authors.lastname: Balík
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects* / Q000737:chemistry; D002244:Carbon / Q000009:adverse effects; D019544:Equipment Failure Analysis; D005549:Foreign-Body Reaction / Q000209:etiology / Q000473:pathology*; D005593:Fracture Fixation, Internal / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods; D007595:Joint Prosthesis / Q000009:adverse effects*; D008422:Materials Testing; D011108:Polymers / Q000009:adverse effects; D011475:Prosthesis Failure; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000601:surgery*; D013123:Spinal Fusion / Q000009:adverse effects* / Q000295:instrumentation* / Q000379:methods; D013552:Swine; D014025:Titanium / Q000009:adverse effects
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D002244:Carbon; D014025:Titanium
references.citation: Rev Med Liege. 2000 Sep;55(9):839-46
references.pmid: 11105598
references.citation: Cell Transplant. 1995 Sep-Oct;4(5):455-77
references.pmid: 8520830
references.citation: Eur Spine J. 2000 Oct;9(5):410-6
references.pmid: 11057535
references.citation: Biomaterials. 2003 May;24(11):1877-87
references.pmid: 12615478
references.citation: J Biomed Mater Res. 1997 Jun 5;35(3):399-406
references.pmid: 9138074
references.citation: J Mater Sci Mater Med. 2003 Jun;14(6):531-7
references.pmid: 15348437
references.citation: J Mater Sci Mater Med. 2000 Dec;11(12):793-8
references.pmid: 15348062
references.citation: J Biomed Mater Res B Appl Biomater. 2003 Feb 15;64(2):107-20
references.pmid: 12516085