[Expression of VEGF-C and VEGFR-3 in human laryngeal squamous cell carcinomas and cervical metastatic lymph node tissues].
OBJECTIVE
To study the gene expression of vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) in laryngeal squamous carcinoma and their relation with clinic pathologic features.


METHODS
VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma (PLC) and cervical lymph node (CLN) were examined by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
The gene expression of VEGF-C and VEGFR-3 are different among normal laryngeal mucosa tissue, primary laryngeal carcinoma and cervical lymph node in the same patient. In primary laryngeal carcinoma, expression of VEGF-C, VEGFR-3 were significantly higher in lymph node positive group than in lymph node negative group and associated with histological grade of differentiation; The gene expression of VEGF-C, VEGFR-3 were not associated with age, sex, site, T stage.


CONCLUSIONS
VEGF-C, VEGFR-3 have prognostic significance in laryngeal carcinoma.
Wang
Zhongliang
Z
Department of Human Anatomy, West China of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
Chen
Yao
Y
Liang
Chuanyu
C
Zhou
Bin
B
Li
Ruixiang
R
Zhang
Lin
L
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000556:secondary; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009333:Neck; D011379:Prognosis; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000096:biosynthesis*
D042582:Vascular Endothelial Growth Factor C; D040321:Vascular Endothelial Growth Factor Receptor-3