title: African swine fever virus infection disrupts centrosome assembly and function.
abstract: African swine fever virus (ASFV) is a large, enveloped DNA virus that assembles in perinuclear sites located close to the centrosome. It is reported here that the microtubule network becomes disorganized soon after the onset of viral DNA replication and formation of assembly sites. ASFV infection resulted in loss of gamma-tubulin and pericentrin at the centrosome; this was due to protein relocalization, but not degradation. ASFV infection also inhibited the ability of the centrosome to nucleate microtubules. The reorganization of microtubules seen in ASFV-infected cells may therefore be mediated by gamma-tubulin and pericentrin redistribution, and consequent disruption of centrosome assembly and function.
authors.affiliation: Department of Immunology and Pathology, Pirbright Laboratories, Institute for Animal Health, Ash Road, Woking, Surrey GU24 0NF, UK.
authors.forename: Nolwenn
authors.initials: N
authors.lastname: Jouvenet
authors.affiliation: Department of Immunology and Pathology, Pirbright Laboratories, Institute for Animal Health, Ash Road, Woking, Surrey GU24 0NF, UK.
authors.forename: Thomas
authors.initials: T
authors.lastname: Wileman
mesh_terms: D000358:African Swine Fever Virus / Q000502:physiology*; D000818:Animals; D000941:Antigens / Q000378:metabolism / Q000502:physiology; D018385:Centrosome / Q000378:metabolism* / Q000502:physiology; D002522:Chlorocebus aethiops; D008870:Microtubules / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism / Q000502:physiology; D014709:Vero Cells / Q000821:virology*; D019065:Virus Assembly
chemical_list: D000941:Antigens; D014404:Tubulin; C086178:pericentrin