title: Simultaneous binding of meso-tetrakis(N-methylpyridinium-4-yl)porphyrin and 4',6-diamidino-2-phenylindole at the minor grooves of poly(dA).poly(dT) and poly[d(A-T)(2)]: fluorescence resonance energy transfer between DNA bound drugs.
abstract: The spectral properties of meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP) bound to poly(dA).poly(dT) and poly[d(A-T)(2)] in the presence and in the absence of 4',6-diamidino-2-phenylindole (DAPI) have been studied. DAPI fits deeply into the minor groove of both poly(dA).poly(dT) and poly[d(A-T)(2)], and TMPyP is also situated at the minor groove. The nature of the absorption, circular dichroism (CD), and flow linear dichroism (LD) spectra of the TMPyP-poly(dA).poly(dT) and -poly[d(A-T)(2)] complexes in the Soret band is essentially unaffected whether the minor groove is blocked by DAPI or not, although small variations been noticed in the presence of DAPI. Furthermore, a close analysis of the reduced LD spectrum in the Soret band results in angles of approximately 80 degrees and 55 degrees between transition moments of the TMPyP and DNA helix axes in the absence of DAPI. All these observations indicate that the side of TMPyP whose structure resembles that of classical minor groove binding drugs does not fit deeply into the minor groove. This suggests that TMPyP binds across the minor groove: two positively charged pyridiniumyl rings interact electrostatically with negatively charged phosphate groups of DNA. When DAPI and TMPyP are simultaneously bound to poly(dA).poly(dT) or poly[d(A-T)(2)], the fluorescence intensity of DAPI decreases as TMPyP concentration increases, indicating that the excited energy of DAPI is transferred to TMPyP.
authors.affiliation: Department of Chemistry, Yeungnam University, 214-1 Dae-dong, Kyoungsan City, Kyoung-buk, 712-749, Republic of Korea.
authors.forename: Biao
authors.initials: B
authors.lastname: Jin
authors.forename: Hyun Mee
authors.initials: HM
authors.lastname: Lee
authors.forename: Young-Ae
authors.initials: YA
authors.lastname: Lee
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Ko
authors.forename: Cheol
authors.initials: C
authors.lastname: Kim
authors.forename: Seog K
authors.initials: SK
authors.lastname: Kim
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011067:Poly dA-dT / Q000737:chemistry* / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007211:Indoles; D011089:Polydeoxyribonucleotides; D011166:Porphyrins; D011067:Poly dA-dT; C021096:tetra(4-N-methylpyridyl)porphine; C007293:DAPI; C084838:polydeoxyadenylic acid-polythymidylic acid; D004247:DNA