Time course study of in situ expression of antigens following DNA-vaccination against VHS in rainbow trout (Oncorhynchus mykiss Walbaum) fry.
The present study was performed as a time course study of fish vaccinated with 20 microg plasmid DNA vaccine encoding either the VHSV G-protein or the VHSV N-protein. Samples of the injection site were collected sequentially over a 7-week period. The study revealed an intense positive staining by immunohistochemistry for the viral G-protein mainly in the membrane of intact myocytes, most prominent by days 10-27, and with concomitant infiltration of inflammatory cells by days 13-38 that subsequently lead to a marked reduction in the number of myocytes expressing the G-protein. By immunofluorescence, infiltrating cells positive for MHC II, IgM, and C3 were demonstrated. By contrast, in fish vaccinated with the VHSV-N construct, fewer, diffusely positive myocytes were found, most prominent by days 13-38, these having a positive reaction for the N-protein mainly in the cytoplasm and variably in the membrane. N-protein positive myocytes did not attract infiltrating cells to the same degree. Positive reaction for the N-protein almost ceased by day 48 post-vaccination.
Lorenzen
Ellen
E
Danish Institute for Food and Veterinary Research, Hangøvej 2, DK-8200 Arhus N, Denmark. el@dfvf.dk
Lorenzen
Niels
N
Einer-Jensen
Katja
K
Brudeseth
Bjørn
B
Evensen
Oystein
O
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D029141:Novirhabdovirus / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000821:virology; D013997:Time Factors; D019444:Vaccines, DNA / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology*
D000956:Antigens, Viral; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines