Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence.
By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding proteins. In a mouse infection model, both mutants were attenuated in virulence, suggesting that M. tuberculosis encounters limiting phosphate concentrations during its intracellular life span.
Peirs
Priska
P
Pasteur Institute of Brussels, Engelandstraat 642, B-1180-Brussels, Belgium.
Lefèvre
Philippe
P
Boarbi
Samira
S
Wang
Xiao-Ming
XM
Denis
Olivier
O
Braibant
Martine
M
Pethe
Kevin
K
Locht
Camille
C
Huygen
Kris
K
Content
Jean
J
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D017353:Gene Deletion; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity*; D028044:Phosphate-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D014376:Tuberculosis / Q000382:microbiology* / Q000503:physiopathology; D014774:Virulence
D001426:Bacterial Proteins; D003470:Culture Media; D028044:Phosphate-Binding Proteins; D010710:Phosphates
Mol Microbiol. 2000 Jun;36(5):1024-33
10844688
Infect Immun. 1992 Jul;60(7):2880-6
1612754
FEMS Microbiol Rev. 2000 Oct;24(4):449-67
10978546
FEMS Microbiol Lett. 2001 Feb 20;195(2):121-6
11179639
J Bacteriol. 2001 Sep;183(17):5008-14
11489853
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7877-82
12788972
Structure. 2003 Jul;11(7):765-74
12842040
Nat Med. 2003 Nov;9(11):1356-7
14595429
Microbiology. 2003 Nov;149(Pt 11):3203-12
14600232
J Bacteriol. 1977 Aug;131(2):505-11
328484
J Bacteriol. 1980 Oct;144(1):356-65
6998957
J Bacteriol. 1981 Oct;148(1):1-9
7026529
J Bacteriol. 1985 Jan;161(1):189-98
3881386
Infect Immun. 1989 Aug;57(8):2481-8
2545626
J Gen Microbiol. 1990 Mar;136(3):477-80
2118164
Infect Immun. 1993 Jun;61(6):2687-93
8500908
J Biol Chem. 1994 Jan 21;269(3):1956-8
8294447
Infect Immun. 1994 Mar;62(3):849-54
8112854
Kidney Int. 1996 Apr;49(4):964-7
8691745
FEBS Lett. 1996 Sep 30;394(2):206-12
8843165
Int Rev Cytol. 1992;137:1-35
1385348
Annu Rev Cell Biol. 1992;8:67-113
1282354
J Cell Biochem. 1993 Jan;51(1):47-54
8432742
Gene. 1996 Oct 17;176(1-2):171-6
8918249
Eur J Biochem. 1997 Mar 1;244(2):604-12
9119030
J Bacteriol. 1997 Apr;179(8):2534-9
9098050
J Bacteriol. 1997 May;179(9):2900-6
9139906
Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10955-60
9380741
Nature. 1998 Jun 11;393(6685):537-44
9634230
J Mol Biol. 1999 Apr 2;287(3):467-84
10092453
Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5433-7
2052623
FEMS Microbiol Lett. 2000 Jul 15;188(2):135-9
10913696