Candida albicans double-stranded DNA can participate in the host defense against disseminated candidiasis.
In the present work, we studied the in vitro immunomodulatory properties of double-stranded Candida albicans DNA and its protective effect in murine disseminated candidiasis. DNA induced the production of TNF-alpha by peritoneal macrophages and splenocytes in vitro through a chloroquine-dependent mechanism. Yeast DNA acted synergistically with IFN-gamma in triggering the secretion of nitric oxide by macrophages and enabled them to stimulate the proliferation of T cells in response to soluble anti-CD3. The effect of DNA on splenocytes is associated with an enhanced synthesis of IFN-gamma, IL-2 and IL-10. In vivo, DNA decreased the mortality and lowered the kidney contamination in mice intraperitoneally inoculated with C. albicans simultaneously with an increase in the specific proliferative response and cytokine production. The present results indicate that C. albicans DNA can provide protection against disseminated infection.
Yordanov
Martin
M
Department of Immunology, Institute of Microbiology, 26. G. Bonchev Street, 1113 Sofia, Bulgaria.
Dimitrova
Petya
P
Danova
Svetla
S
Ivanovska
Nina
N
D000818:Animals; D002176:Candida albicans / Q000235:genetics* / Q000276:immunology; D002177:Candidiasis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004271:DNA, Fungal / Q000494:pharmacology* / Q000627:therapeutic use; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D016207:Cytokines; D004271:DNA, Fungal; D014409:Tumor Necrosis Factor-alpha