Resetting of peripheral circadian clock by prostaglandin E2.
In mammals, the master circadian pacemaker is located in the suprachiasmatic nucleus (SCN) of the hypothalamus. The SCN is thought to drive peripheral oscillators by controlling neuronal and humoral signals that can entrain the peripheral clocks. Here, we show that prostaglandin E2 (PGE2), a proinflammatory compound known to have diverse biological effects, is able to act as an in vivo clock-resetting agent. We find that in cultured NIH3T3 fibroblasts, PGE2 is able to induce transient expression of Period 1 messenger RNA and the following circadian oscillation of clock gene expression. Furthermore, we demonstrate that intraperitoneal administration of PGE2 results in the phase shift of circadian gene expression in mouse peripheral tissues in a time-dependent manner. This phase shift is also induced by the EP1/EP3 agonist sulprostone but not by the EP2 agonist butaprost. The PGE2-induced phase shift is inhibited by the EP1 antagonist SC-51322. These results suggest that PGE2 acts as an in vivo clock-resetting factor by means of the EP1 subtype of PGE receptors.
Tsuchiya
Yoshiki
Y
Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan.
Minami
Itsunari
I
Kadotani
Hiroshi
H
Nishida
Eisuke
E
D000818:Animals; D001683:Biological Clocks / Q000187:drug effects* / Q000502:physiology*; D018797:Cell Cycle Proteins; D015232:Dinoprostone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000235:genetics; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000378:metabolism; D058306:Receptors, Prostaglandin E, EP1 Subtype
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; C545636:Ptger1 protein, mouse; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E; D058306:Receptors, Prostaglandin E, EP1 Subtype; D015232:Dinoprostone
Genes Dev. 2000 Mar 15;14(6):645-9
10733524
Physiol Rev. 1999 Oct;79(4):1193-226
10508233
Mol Cell Biol. 2000 Jul;20(13):4773-81
10848603
Neuroreport. 2000 Jul 14;11(10):2127-31
10923657
Science. 2000 Sep 29;289(5488):2344-7
11009419
Curr Biol. 2000 Oct 19;10(20):1291-4
11069111
Annu Rev Physiol. 2001;63:647-76
11181971
Cell. 2001 Jun 29;105(7):877-89
11439184
Annu Rev Biochem. 2002;71:473-510
12045104
Cell. 2002 Jul 26;110(2):251-60
12150932
Genes Cells. 2003 Aug;8(8):713-20
12875656
Int J Biochem Cell Biol. 2004 Jul;36(7):1187-205
15109566
J Biol Chem. 1992 Apr 5;267(10):6463-6
1372606
J Biol Chem. 1993 Sep 25;268(27):20175-8
7690750
Biochem Biophys Res Commun. 1995 Aug 15;213(2):625-9
7646520
Bone. 1995 Jul;17(1):79-83
7577162
Biochim Biophys Acta. 1995 Oct 26;1259(1):109-19
7492609
Proc Natl Acad Sci U S A. 1996 May 14;93(10):4885-90
8643498
Cell. 1997 Dec 26;91(7):1043-53
9428526
Cell. 1998 Jun 12;93(6):929-37
9635423
J Biol Chem. 1999 Jan 1;274(1):75-85
9867813
Science. 2000 Apr 28;288(5466):682-5
10784453