[The study of the pro-nucleating activity of bacteria identified in cholesterol gallstones in model bile systems].
OBJECTIVE
To explore the relationship of bacteria identified in cholesterol gallstones and gallstone formation.


METHODS
Observe the bacteria activity in model bile and the influence of bacteria on the cholesterol nucleation time (NT).


RESULTS
(1) Model bile were suitable for the growth of E. coli, Pseudomonas aeruginosa, staphylococcus aureus, enterococcus faecalis, clostridium difficile and Clostridium. Propionibacterium acne grew weakly and the growth of Bacteroides fragilis was restrained in model bile. (2) Only pseudomonas aeruginosa and enTerococcus faecalis could ly shorten the cholesterol nucleation time. (3) With pseudomonas aeruginosa or enTerococcus faecalis added in model bile, the formation of cholesterol crystals presented a progressive course of evolution.


CONCLUSIONS
Pseudomonas aeruginosa and enterococcus faecalis, not propionibacterium acne, have pro-nucleating ability in model bile.
Zhu
Lei-ming
LM
Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
Cai
Duan
D
Lü
Yuan
Y
Chen
Wei-huan
WH
Wang
Wen-feng
WF
Zhang
Yan-ling
YL
D001646:Bile / Q000378:metabolism / Q000382:microbiology*; D002769:Cholelithiasis / Q000382:microbiology*; D002784:Cholesterol / Q000378:metabolism*; D003460:Crystallization; D013293:Enterococcus faecalis / Q000254:growth & development; D008954:Models, Biological; D011425:Propionibacterium acnes / Q000254:growth & development; D011550:Pseudomonas aeruginosa / Q000254:growth & development
D002784:Cholesterol