Early expression of AMPA receptors and lack of NMDA receptors in developing rat climbing fibre synapses.
Whether nascent glutamatergic synapses acquire their AMPA receptors constitutively or via a regulated pathway triggered by pre-existing NMDA receptor activation is still an open issue. Here, we provide evidence that some glutamatergic synapses develop without expressing NMDA receptors. Using immunocytochemistry, we showed that synapses between developing rat climbing fibres and Purkinje cells expressed GluR2-containing AMPA receptors as soon as they were formed (i.e. on embryonic day 19) but never carried detectable NMDA receptors. This was confirmed by electrophysiological recordings. Excitatory synaptic currents were recorded in Purkinje cells as early as P0. However, no NMDA receptor-mediated component was found in either spontaneous or evoked synaptic responses. In addition, we ruled out a possible role of extrasynaptic NMDA receptors by showing that AMPA receptor clustering at nascent climbing fibre synapses was not modified by chronic in utero NMDA receptor blockade.
Lachamp
Philippe
P
Laboratoire de Neurophysiologie cellulaire, UMR CNRS 6150, Centre National de la Recherche Scientifique et Université de la Mediterrannée, IFR Jean Roche, Faculté de Médecine Nord, Bd Pierre Dramard, F13916 Marseille cedex 20, France.
Balland
Bénedicte
B
Tell
Fabien
F
Baude
Agnès
A
Strube
Caroline
C
Crest
Marcel
M
Kessler
Jean-Pierre
JP
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000196:embryology* / Q000378:metabolism*; D005260:Female; D009415:Nerve Net / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013569:Synapses / Q000502:physiology*
D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate
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