A short peptide GPIGS promotes proliferation of hair bulb keratinocytes and accelerates hair regrowth in mice.
The aim of this study was to discover a novel agent that promotes hair growth. We carried out a screening test in 298 types of conditioned medium (CM) from cultures of bacteria by using a hair bulb keratinocyte (HBK) growth assay. As a result, we found a HBK growth factor in the CM of Bacillus sp. M18. This HBK growth factor was purified by collecting biologically active fractions in three steps, including HP-20 batch processing, LH-20 chromatography and C18 reverse-phase high-pressure liquid chromatography, and identified as a short peptide GPIGS. GPIGS increased Akt phosphorylation in HBKs. Moreover, the GPIGS-stimulated HBK growth was inhibited by the treatment with LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI-3K). These results suggest that GPIGS promotes HBK growth via the PI-3K/Akt pathway. In addition to in vitro tests, GPIGS was found to accelerate hair regrowth in telogen mice. Our results indicate that GPIGS is a potential agent to promote hair growth.
Tsuruda
Akinori
A
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
Kawano
Yasuhiro
Y
Maekawa
Takaaki
T
Oka
Syuichi
S
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D006133:Growth Substances / Q000138:chemical synthesis / Q000494:pharmacology*; D018859:Hair Follicle / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*
D006133:Growth Substances; D010446:Peptide Fragments