title: Transient RNAi induction against endogenous genes in Arabidopsis protoplasts using in vitro-prepared double-stranded RNA.
abstract: RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
authors.forename: Chung-Il
authors.initials: CI
authors.lastname: An
authors.forename: Aki
authors.initials: A
authors.lastname: Sawada
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Ei-ichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Akio
authors.initials: A
authors.lastname: Kobayashi
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D002478:Cells, Cultured; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D011523:Protoplasts / Q000502:physiology*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000502:physiology*; D013997:Time Factors
chemical_list: D012330:RNA, Double-Stranded