WRKY group IId transcription factors interact with calmodulin.
Calmodulin (CaM) is a ubiquitous Ca(2+)-binding protein known to regulate diverse cellular functions by modulating the activity of various target proteins. We isolated a cDNA encoding AtWRKY7, a novel CaM-binding transcription factor, from an Arabidopsis expression library with horseradish peroxidase-conjugated CaM. CaM binds specifically to the Ca(2+)-dependent CaM-binding domain (CaMBD) of AtWRKY7, as shown by site-directed mutagenesis, a gel mobility shift assay, a split-ubiquitin assay, and a competition assay using a Ca2+/CaM-dependent enzyme. Furthermore, we show that the CaMBD of AtWRKY7 is a conserved structural motif (C-motif) found in group IId of the WRKY protein family.
Park
Chan Young
CY
Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsarg National University, Jinju 660-701, Republic of Keorea.
Lee
Ju Huck
JH
Yoo
Jae Hyuk
JH
Moon
Byeong Cheol
BC
Choi
Man Soo
MS
Kang
Yun Hwan
YH
Lee
Sang Min
SM
Kim
Ho Soo
HS
Kang
Kyu Young
KY
Chung
Woo Sik
WS
Lim
Chae Oh
CO
Cho
Moo Je
MJ
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism
D029681:Arabidopsis Proteins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D010446:Peptide Fragments; D014157:Transcription Factors; D025801:Ubiquitin; C498414:WRKY7 protein, Arabidopsis