title: The genotype of Toxoplasma gondii strains causing ocular toxoplasmosis in humans in Brazil.
abstract: OBJECTIVE
  To determine Toxoplasma gondii genotype in scars of retinochoroiditis in humans.
  
  
  METHODS
  Immunopathology analysis.
  
  
  METHODS
  DNA was isolated from retinochoroidal specimens taken from the borders of the scars from eye bank eyes as well as enucleated eyes from uveitis patients with retinochoroidal toxoplasmic scars. The SAG2 region was amplified by polymerase chain reaction and analyzed by restriction fragment length polymorphism.
  
  
  RESULTS
  Of the 92 eyes studied, 9 had toxoplasmic scars. All of them, as well as the 2 enucleated eyes, disclosed the presence of type I parasites by the gel electrophoresis analysis of the restriction fragment length polymorphism from SAG2.
  
  
  CONCLUSIONS
  These results suggest that type I strain seems to be responsible for the ocular infections in the population studied.
authors.affiliation: Department of Immunology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Adriana Lima
authors.initials: AL
authors.lastname: Vallochi
authors.forename: Cristina
authors.initials: C
authors.lastname: Muccioli
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Martins
authors.forename: Cláudio
authors.initials: C
authors.lastname: Silveira
authors.forename: Rubens
authors.initials: R
authors.lastname: Belfort
authors.forename: Luiz Vicente
authors.initials: LV
authors.lastname: Rizzo
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002825:Chorioretinitis / Q000453:epidemiology / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis*; D005125:Eye Banks; D015353:Eye Enucleation; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics; D014122:Toxoplasma / Q000235:genetics*; D014126:Toxoplasmosis, Ocular / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C067547:surface antigen P22, Toxoplasma