Different antibody- and cytokine-mediated responses to Plasmodium falciparum parasite in two sympatric ethnic tribes living in Mali.
The Fulani are known to be less susceptible to Plasmodium falciparum malaria infections and to have lower parasitaemia despite living under similar malaria transmission intensity compared with other ethnic tribes. The aim of the present study was to examine whether the Fulani were more polarised towards Th2 as reflected by higher numbers of malaria-specific IL-4- and IL-10-producing cells and lower numbers of IFN-gamma- and IL-12-producing cells as compared to their neighbour ethnic tribe, the Dogon of Mali. Total IgE and both anti-malaria IgE and IgG antibodies were measured by ELISA and the numbers of IL-4-, IFN-gamma-, IL-10- and IL-12-producing cells were enumerated using enzyme-linked ImmunoSpot assay (ELISPOT). Numbers of parasite clones were detected by polymerase chain reaction (PCR). The study was performed outside the transmission period and all individuals included were asymptomatic. The results revealed that the Fulani were less parasitised, had fewer circulating parasite clones in their blood, had significantly higher anti-malaria IgG and IgE antibodies and higher proportions of malaria-specific IL-4- and IFN-gamma-producing cells compared to the Dogon. The higher antigen-specific production of IL-4 among the Fulani was statistically significant both before and after adjustment for level of spontaneous cytokine production, while greater IFN-gamma production only attained statistical significance after adjustment for spontaneous levels. Taken together, the association of higher anti-malarial IgE and IgG antibodies and increased numbers of specific IL-4- and IFN-gamma-producing cells compared to the ethnic sympatric tribe, the Dogon, may assist in explaining the lower susceptibility to malaria observed in the Fulani.
Farouk
Salah E
SE
Department of Immunology, Wenner-Gren Institute, Stockholm University, Svante Arrheniusväg 16, S-10691 Stockholm, Sweden.
Dolo
Amagana
A
Bereczky
Sàndor
S
Kouriba
Bourema
B
Maiga
Boubacar
B
Färnert
Anna
A
Perlmann
Hedvig
H
Hayano
Masashi
M
Montgomery
Scott M
SM
Doumbo
Ogobara K
OK
Troye-Blomberg
Marita
M
D000328:Adult; D000368:Aged; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004198:Disease Susceptibility; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D018655:Lymphocyte Count; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000208:ethnology / Q000276:immunology*; D008297:Male; D008302:Mali / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D044382:Population Groups; D018418:Th2 Cells / Q000276:immunology*
D000913:Antibodies, Protozoan; D016207:Cytokines; D016054:DNA, Protozoan; D007073:Immunoglobulin E