Uterotrophic effects of benzophenone derivatives and a p-hydroxybenzoate used in ultraviolet screens.
Ultraviolet (UV) sunscreen products are popular because of concerns about UV radiation and skin cancer. Unfortunately, some of these products contain agents with estrogenic activity. We used an ovariectomized rat uterotrophic assay to measure the estrogenic activities of 2,4-dihydroxybenzophenone (2,4-DHBP), 2,2',4,4'-tetrahydroxybenzophenone (2,2',4,4'-THBP), and 4-hydroxybenzoic acid isobutyl ester (isobutyl-paraben), which are agents in UV sunscreens, and ethynyl estradiol (EE) and bisphenol A (BPA), which are positive controls. All chemicals increased rat uterine weights. The 10% effective doses (ED10, mg/kg/day) of EE, BPA, 2,4-DHBP, 2,2',4,4'-THBP, and isobutyl-paraben, as determined by Hill equation analysis, where 5E-5, 41.1, 544.6, 33.0, and 230.9, respectively, and their relative potencies against EE were about 1/800,000, 1/10,000,000, 1/600,000, and 1/4,000,000, respectively. Our findings indicated that UV screens contain weak estrogenic compounds.
Koda
Tomoko
T
Endocrine Disrupters Research Laboratory, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan. kouda.tomoko@nies.go.jp
Umezu
Toyoshi
T
Kamata
Ryo
R
Morohoshi
Kaori
K
Ohta
Toshiko
T
Morita
Masatoshi
M
D000818:Animals; D001559:Benzhydryl Compounds; D001577:Benzophenones / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000633:toxicity; D004997:Ethinyl Estradiol / Q000633:toxicity; D005260:Female; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D010226:Parabens / Q000633:toxicity*; D010636:Phenols / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013473:Sunscreening Agents / Q000633:toxicity*; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D001577:Benzophenones; D004968:Estrogens, Non-Steroidal; D004997:Ethinyl Estradiol; D010226:Parabens; D010636:Phenols; D013473:Sunscreening Agents; C471067:isobutylparaben; C038193:4-hydroxybenzoic acid; C006780:bisphenol A; D000098826:Bisphenol A Compounds