title: Efficient immobilization of enzymes on microchannel surface through His-tag and application for microreactor.
abstract: We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes.
authors.affiliation: Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Saga, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Jun
authors.initials: J
authors.lastname: Kaneno
authors.forename: Susumu
authors.initials: S
authors.lastname: Yamaori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Honda
authors.forename: Maria Portia P
authors.initials: MP
authors.lastname: Briones
authors.forename: Masato
authors.initials: M
authors.lastname: Uehara
authors.forename: Kazunari
authors.initials: K
authors.lastname: Arima
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kanno
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamashita
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Hiroo
authors.initials: H
authors.lastname: Yonezawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maeda
mesh_terms: D000818:Animals; D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006639:Histidine / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D006639:Histidine; D007770:L-Lactate Dehydrogenase