Non-specific tolerance induced by staphylococcal enterotoxin B in treating high risk corneal transplantation in rats.
OBJECTIVE
To explore the role of staphylococcal enterotoxin B (SEB) in treating high risk corneal keratoplasty in rats.


METHODS
Rat corneal high risk transplantation rejection models were set up using Fisher 344 and Lewis rats. The experimental rats were injected intraperitoneally with 0.2 ml SEB at different concentrations before keratoplasty. The rejection indexes of the allograft were recorded and the lymphocyte infiltration in the allograft and the percentage of the lymphocyte subpopulation in the lymphatic organs were also examined. Lymphocyte proliferation ability and the concentration of IL-2 and IL-10 in the serum were also evaluated.


RESULTS
Compared with the control group, SEB prolonged the survival time of the allograft significantly from 7 to 12 days. It could also reduce CD4(+) and CD8(+) lymphocyte infiltration in the allograft and minimise the percentage of CD4(+) and CD8(+) lymphocytes in the lymphatic organs. The lymphocyte proliferation ability was also weakened. However, the percentage of CD4(+) NK T lymphocytes in the lymphatic organs was raised. The serum concentration of IL-10 was higher but IL-2 was lower in the SEB treated groups.


CONCLUSIONS
SEB prolonged the survival time of the allograft in high risk rat corneal allo-transplantation, which may be caused by T cell deletion and acquisition of non-specific tolerance.
Jie
Y
Y
Beijing Institute of Ophthalmology, TongRen Eye Center, TongRen Hospital, Capital University of Medical Sciences, China.
Pan
Z
Z
Chen
Y
Y
Wei
Y
Y
Zhang
W
W
Wu
Y
Y
Peng
H
H
Xu
L
L
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016039:Corneal Transplantation; D004768:Enterotoxins / Q000627:therapeutic use*; D005260:Female; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D007694:Killer Cells, Natural / Q000276:immunology; D018655:Lymphocyte Count; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D004768:Enterotoxins; D007376:Interleukin-2; D016753:Interleukin-10; C031818:enterotoxin B, staphylococcal
Eur J Immunol. 1998 May;28(5):1534-43
9603458
Br J Ophthalmol. 1997 Sep;81(9):778-84
9422933
J Immunol. 1999 Jun 1;162(11):6312-5
10352241
Cornea. 2002 Mar;21(2 Suppl 1):S33-8
11995808
Int Rev Immunol. 2002 Mar-Jun;21(2-3):173-96
12424842
Eur Cytokine Netw. 2003 Jan-Mar;14(1):4-14
12799208
Cell Immunol. 2003 Apr;222(2):144-55
12826084
Clin Immunol. 2003 Jul;108(1):51-9
12865071
Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3346-51
12882780
J Immunol. 2003 Sep 1;171(5):2571-80
12928408
Invest Ophthalmol Vis Sci. 1985 Jan;26(1):23-30
3881365
Adv Immunol. 1990;48:191-226
2190450
Cornea. 1991 Sep;10(5):374-80
1935133
Invest Ophthalmol Vis Sci. 1996 Nov;37(12):2485-94
8933765
Transplantation. 1997 May 27;63(10):1501-7
9175817
Curr Opin Immunol. 1998 Dec;10(6):656-62
9914224