Autologous transplantation of porcine myogenic precursor cells in skeletal muscle.
Myoblast transplantation is a potential therapy for severe muscle trauma, myopathies and heart infarct. Success with this therapy relies on the ability to obtain cell preparations enriched in myogenic precursor cells and on their survival after transplantation. To define myoblast transplantation strategies applicable to patients, we used a large animal model, the pig. Muscle dissociation procedures adapted to porcine tissue gave high yields of cells containing at least 80% myogenic precursor cells. Autologous transplantation of 3[H]-thymidine labeled porcine myogenic precursor cells indicated 60% survival at day 1 followed by a decay to 10% at day 5 post-injection. Nuclei of myogenic precursor cells transduced with a lentivirus encoding the nls-lacZ reporter gene were present in host myotubes 8 days post-transplantation, indicating that injected myogenic precursor cells contribute to muscle regeneration. This work suggests that pig is an adequate large animal model for exploring myogenic precursor cells transplantation strategies applicable in patients.
Holzer
Nicolas
N
Department of Clinical Neurosciences and Dermatology, Geneva Faculty of Medicine and University Hospital, Geneva, Switzerland.
Hogendoorn
Simone
S
Zürcher
Line
L
Garavaglia
Guido
G
Yang
Sheng
S
König
Stephane
S
Laumonier
Thomas
T
Menetrey
Jacques
J
D000818:Animals; D019002:CD56 Antigen / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D003893:Desmin / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D007211:Indoles; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000502:physiology / Q000637:transplantation*; D032446:Myoblasts / Q000502:physiology / Q000637:transplantation*; D013552:Swine; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014161:Transduction, Genetic; D014182:Transplantation, Autologous; D014316:Tritium / Q000378:metabolism
D019002:CD56 Antigen; D003893:Desmin; D007211:Indoles; D014316:Tritium; C007293:DAPI; D013936:Thymidine