Expression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis.
BACKGROUND
The human psoriasin (S100A7) gene has been implicated in inflammation and tumor progression. Implementation of a mouse model would facilitate further investigation of its function, however little is known of the murine psoriasin gene. In this study we have cloned the cDNA and characterized the expression of the potential murine ortholog of human S100A7/psoriasin in skin inflammation and mammary tumorigenesis.


METHODS
On the basis of chromosomal location, phylogenetic analysis, amino acid sequence similarity, conservation of a putative Jab1-binding motif, and similarities of the patterns of mouse S100A7/psoriasin gene expression (measured by RT-PCR and in-situ hybridization) with those of human S100A7/psoriasin, we propose that mouse S100A7/psoriasin is the murine ortholog of human psoriasin/S100A7.


RESULTS
Although mouse S100A7/psoriasin is poorly conserved relative to other S100 family members, its pattern of expression parallels that of the human psoriasin gene. In murine skin S100A7/psoriasin was significantly upregulated in relation to inflammation. In murine mammary gland expression is also upregulated in mammary tumors, where it is localized to areas of squamous differentiation. This mirrors the context of expression in human tumor types where both squamous and glandular differentiation occur, including cervical and lung carcinomas. Additionally, mouse S100A7/psoriasin possesses a putative Jab1 binding motif that mediates many downstream functions of the human S100A7 gene.


CONCLUSIONS
These observations and results support the hypothesis that the mouse S100A7 gene is structurally and functionally similar to human S100A7 and may offer a relevant model system for studying its normal biological function and putative role in tumor progression.
Webb
Meghan
M
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada. MeghanWebb@hotmail.com
Emberley
Ethan D
ED
Lizardo
Michael
M
Alowami
Salem
S
Qing
Gefei
G
Alfia'ar
Abdullah
A
Snell-Curtis
Linda J
LJ
Niu
Yulian
Y
Civetta
Alberto
A
Myal
Yvonne
Y
Shiu
Robert
R
Murphy
Leigh C
LC
Watson
Peter H
PH
D015127:9,10-Dimethyl-1,2-benzanthracene; D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D003436:Croton Oil; D003872:Dermatitis / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins
D000199:Actins; D002135:Calcium-Binding Proteins; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human; C506174:S100a7a protein, mouse; D015127:9,10-Dimethyl-1,2-benzanthracene; D003436:Croton Oil
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