The expression of the rice (Oryza sativa L.) homologue of Snm1 is induced by DNA damages.
We isolated and characterized the rice homologue of the DNA repair gene Snm1 (OsSnm1). The length of the cDNA was 1862bp; the open reading frame encoded a predicted product of 485 amino acid residues with a molecular mass of 53.2kDa. The OsSnm1 protein contained the conserved beta-lactamase domain in its internal region. OsSnm1 was expressed in all rice organs. The expression was induced by MMS, H(2)O(2), and mitomycin C, but not by UV. Transient expression of an OsSnm1/GFP fusion protein in onion epidermal cells revealed the localization of OsSnm1 to the nucleus. These results suggest that OsSnm1 is involved not only in the repair of DNA interstrand crosslinks, but also in various other DNA repair pathways.
Kimura
Seisuke
S
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba 278-8510, Japan.
Saotome
Ai
A
Uchiyama
Yukinobu
Y
Mori
Yoko
Y
Tahira
Yasue
Y
Sakaguchi
Kengo
K
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D002478:Cells, Cultured; D004249:DNA Damage / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism*; D014018:Tissue Distribution
D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C499103:SNM1 protein, Arabidopsis