Nitrile bioconversion by Microbacterium imperiale CBS 498-74 resting cells in batch and ultrafiltration membrane bioreactors.
The biohydration of acrylonitrile, propionitrile and benzonitrile catalysed by the NHase activity contained in resting cells of Microbacterium imperiale CBS 498-74 was operated at 5, 10 and 20 degrees C in laboratory-scale batch and membrane bioreactors. The bioreactions were conducted in buffered medium (50 mM Na(2)HPO(4)/NaH(2)PO(4), pH 7.0) in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. The integral bioreactor performances were studied with a cell loading (dry cell weight; DCW) varying from 0.1 mg(DCW) per reactor to 16 mg(DCW) per reactor, in order to realize near 100% bioconversion of acrylonitrile, propionitrile and benzonitrile without consistent loss of NHase activity.
Cantarella
M
M
Department of Chemistry, Chemical Engineering and Materials, University of L'Aquila, Monteluco di Roio, L'Aquila, 67040, Italy. cantarel@ing.univaq.it
Cantarella
L
L
Gallifuoco
A
A
Spera
A
A
D000181:Acrylonitrile / Q000378:metabolism*; D000192:Actinomycetales / Q000201:enzymology / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D006836:Hydro-Lyases / Q000032:analysis; D008566:Membranes / Q000382:microbiology; D009570:Nitriles / Q000378:metabolism*; D013696:Temperature; D014462:Ultrafiltration / Q000379:methods
D003470:Culture Media; D009570:Nitriles; C014356:benzonitrile; C005557:propionitrile; D006836:Hydro-Lyases; C048831:nitrile hydratase; D000181:Acrylonitrile
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