Frequent mutation related with overexpression of DNA polymerase beta in primary tumors and precancerous lesions of human stomach.
To explore whether DNA polymerase beta (pol beta) contributes to the malignant transformation of gastric mucosa, we examined pol beta in gastric tumor cell lines, primary tumors and precancerous lesions. Point mutations of pol beta were detected in 6 of 13 cell lines and 23 of 104 tissues including 35.0% (14/40) of gastric cancer (GC), 30.0% (3/10) of dysplasia (Dys), 28.6% (4/14) of intestinal metaplasia (IM) and 10.5% (2/19) of chronic atrophic gastritis (CAG), respectively. A frequent mutation was a T to C transition at nucleotide 889, which was observed in 4 GC cell lines, 7 GC, 2 Dys, and 2 IM. The level of pol beta expression in tumors was higher than that of their matched normal tissues and gradual changes from GC, Dys, CAG to IM. These results indicate that the mutation and overexpression of pol beta may influence the progression during gastric carcinogenesis.
Tan
Xiao-Hui
XH
Beijing Molecular Oncology Laboratory, Beijing Institute for Cancer Research, School of Oncology, Peking University, Da-Hong-Luo-Chang Street 1#, Western District, Beijing 100034, People's Republic of China.
Zhao
Min
M
Pan
Kai-Feng
KF
Dong
Ying
Y
Dong
Bin
B
Feng
Gui-Jian
GJ
Jia
Guang
G
Lu
You-Yong
YY
D000328:Adult; D000368:Aged; D017398:Alternative Splicing; D001483:Base Sequence; D019951:DNA Polymerase beta / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011230:Precancerous Conditions / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D019951:DNA Polymerase beta