title: [Relationship between the expression of insulin-like growth factor I receptors and apoptosis after preliminary chemotherapy, in child and adolescent osteosarcoma].
abstract: BACKGROUND
  The influence of growth factors and apoptosis on the clinical course of neoplasms is of significant interest for oncologists, especially neoplasms with dynamic changes in proliferation, such as child and adolescent osteosarcoma.
  
  
  OBJECTIVE
  To investigate reciprocal relations between the expression of insulin-like growth factor I receptors (IGF-IR) and index apoptosis ("pre-G l /G 0" fraction, DNA histograms) in osteosarcomas before operation and after preliminary chemotherapy.
  
  
  METHODS
  Flow cytometric analysis was carried out in 20 children and adolescents with osteosarcoma (aged 6 to 18 years) after preliminary chemotherapy. The material for investigation was taken during surgery.
  
  
  RESULTS
  It was shown that higher expression of IGF-IR is associated with lower apoptosis index (% of apoptotic cells) and reversely (significant difference p<O, 05 Student's test).
  
  
  CONCLUSIONS
  Investigations of the above relations are significant for better understanding of the influence of IGF-IR on the disease course and on the preliminary evaluation of cytostatic activity by way of apoptosis initiation.
authors.affiliation: Pracownia Cytometrii, Zaklad Immunologii Klinicznej, Instytut Matki i Dziecka, Kasprzaka 17a, 01-211 Warszawa, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Rytwiński
authors.forename: Aleksander
authors.initials: A
authors.lastname: Wnuk
authors.forename: Wojciech
authors.initials: W
authors.lastname: Woźniak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rychłowska
mesh_terms: D000293:Adolescent; D000911:Antibodies, Monoclonal; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology* / Q000601:surgery; D002455:Cell Division / Q000187:drug effects; D002648:Child; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology* / Q000601:surgery; D017526:Receptor, IGF Type 1 / Q000032:analysis* / Q000187:drug effects; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D017526:Receptor, IGF Type 1