[Gas chromatographic analysis of fatty acids in phosphatidylglycerol from needles of two crosses of Chinese Fir].
This paper reports a method for determination of fatty acids in phosphatidylglycerol from needles of two crosses of Chinese Fir with different cold-resistance. Some simplifications and improvements for analytical method of DEAE CL-6B column chromatography and of silica thin layer chromatography were also discussed. After being extracted with chloroform and methanol, phosphatidylglycerol was isolated from the general lipid extract by using DEAE CL-6B IEC and Silica TLC. Quantitative analysis was conducted by gas chromatography on a DB-FFAP (30m x 0.32mm i.d.) column. The results showed that there was a difference in the ratio of fatty acid (18:1+18:2+18:3)/[16:0+16:1(3t)] between the two crosses of Chinese Fir with different cold-resistance, 1.12 for that with higher cold-resistance and 1.25 for that with lower cold-resistance. A special fatty acid 16:1(3t) was found in both of the two crosses.
Bao
H
H
Nanjing Forestry University, Nanjing, 210037.
Li
M
M
Hu
Z
Z
He
Z
Z
Shi
J
J
D002849:Chromatography, Gas / Q000379:methods*; D032486:Cunninghamia / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D010715:Phosphatidylglycerols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*
D005227:Fatty Acids; D010715:Phosphatidylglycerols; D010936:Plant Extracts