title: Local and systemic expression of inducible nitric oxide synthase in comparison with that of cyclooxygenase-2 in rat carrageenin-induced pleurisy.
abstract: Expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) is up-regulated in response to inflammatory stimuli. To evaluate the extent to which local pleural inflammation involves additional site in the pleural cavity and elsewhere, we investigated the time course of the levels of iNOS and its product in the inflammatory and other sites, and compared those with a level of COX-2 in rat carrageenin-induced pleurisy. The exudate and plasma NOx levels rose, reaching peaks at 9 and 14 h, respectively. Both COX-2 and iNOS became detectable in exudate leukocytes, their levels reaching peaks at 3 and 9 h after irritation, respectively. COX-2 was detectable mainly in neutrophils, but iNOS was detectable in both neutrophils and mononuclear leukocytes. Furthermore, iNOS became detectable in neutrophils and mononuclear leukocytes in enlarged parathymic lymph nodes from 3h in addition to those in peripheral blood and Kupffer cells from 3 to 14 h, respectively. The gene product is also detectable in thymic large dendritic cells of pleurisy-induced rats as well as normal control rats. COX-2 became detectable in stellar dendritic cells of the enlarged draining lymph nodes from 14 h. Thus, these gene products were induced in the immediate proximity of regional lymph nodes, and even at a considerable distance of liver by the local inflammatory stimulus. Although their expression pattern was quite different from each other, these gene products were detectable in phagocytic cells.
authors.affiliation: Department of Mediator and Signal Transduction Pharmacology, Kitasato University Graduate School of Medical Sciences, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Fujisawa
authors.forename: Satoko
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Yura
authors.initials: Y
authors.lastname: Ohkubo
authors.forename: Miwako
authors.initials: M
authors.lastname: Matsui
authors.forename: Sayuri
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Michiko
authors.initials: M
authors.lastname: Kawamura
authors.forename: Ko
authors.initials: K
authors.lastname: Hatanaka
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kawakubo
authors.forename: Yoshisuke
authors.initials: Y
authors.lastname: Hiramoto
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Harada
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000737:chemistry* / Q000494:pharmacology; D002452:Cell Count; D051546:Cyclooxygenase 2; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007962:Leukocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D009566:Nitrates / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000187:drug effects; D052247:Nitric Oxide Synthase Type II; D009573:Nitrites / Q000378:metabolism; D010998:Pleurisy / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D009566:Nitrates; D009573:Nitrites; D002351:Carrageenan; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases