title: Non-specific tolerance induced by staphylococcal enterotoxin B in treating high risk corneal transplantation in rats.
abstract: OBJECTIVE
  To explore the role of staphylococcal enterotoxin B (SEB) in treating high risk corneal keratoplasty in rats.
  
  
  METHODS
  Rat corneal high risk transplantation rejection models were set up using Fisher 344 and Lewis rats. The experimental rats were injected intraperitoneally with 0.2 ml SEB at different concentrations before keratoplasty. The rejection indexes of the allograft were recorded and the lymphocyte infiltration in the allograft and the percentage of the lymphocyte subpopulation in the lymphatic organs were also examined. Lymphocyte proliferation ability and the concentration of IL-2 and IL-10 in the serum were also evaluated.
  
  
  RESULTS
  Compared with the control group, SEB prolonged the survival time of the allograft significantly from 7 to 12 days. It could also reduce CD4(+) and CD8(+) lymphocyte infiltration in the allograft and minimise the percentage of CD4(+) and CD8(+) lymphocytes in the lymphatic organs. The lymphocyte proliferation ability was also weakened. However, the percentage of CD4(+) NK T lymphocytes in the lymphatic organs was raised. The serum concentration of IL-10 was higher but IL-2 was lower in the SEB treated groups.
  
  
  CONCLUSIONS
  SEB prolonged the survival time of the allograft in high risk rat corneal allo-transplantation, which may be caused by T cell deletion and acquisition of non-specific tolerance.
authors.affiliation: Beijing Institute of Ophthalmology, TongRen Eye Center, TongRen Hospital, Capital University of Medical Sciences, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jie
authors.forename: Z
authors.initials: Z
authors.lastname: Pan
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Peng
authors.forename: L
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016039:Corneal Transplantation; D004768:Enterotoxins / Q000627:therapeutic use*; D005260:Female; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D007694:Killer Cells, Natural / Q000276:immunology; D018655:Lymphocyte Count; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
chemical_list: D004768:Enterotoxins; D007376:Interleukin-2; D016753:Interleukin-10; C031818:enterotoxin B, staphylococcal
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