Chronological changes of neurofilament 200 kDa immunoreactivity in the lateral olfactory tract after transient forebrain ischemia in gerbils.
This study was carried out to investigate the transient ischemia-induced changes of neurofilament 200 kDa (NF-200) immunoreactivity and protein content in the gerbil lateral olfactory tract (LOT) after 5 min of transient forebrain ischemia. Weak NF-200 immunoreactivity was detectable in the LOT in the sham-operated group. In this group, a few somata of mitral cells showed weak NF-200 immunoreactivity. One day after transient ischemia, NF-200 immunoreactivity in the LOT was increased significantly. NF-200 immunoreactivity in the LOT by 15 days after ischemia was similar to that in the 1 day post-ischemic group. In this time period, strong NF-200 immunoreactivity was expressed in the mitral cell processes, but the immunoreactivity in the mitral cell somata was significantly decreased. Thereafter, NF-200 immunoreactivity in the LOT was decreased significantly by 30 days after ischemic insult. At this time after ischemia, NF-200 immunoreactivity in the mitral cell dendrites was significantly decreased. The result of Western blot study showed that the pattern of NF-200 expression was similar to that of immunohistochemistry after ischemia-reperfusion. Our result suggests that changes of NF-200 protein in the gerbil LOT may be related to response to ischemic damage and that the axonal transport followed transient ischemia may be disturbed.
Lee
Hyeon Yong
HY
School of Biotechnology and Bionengineering, Kangwon National University, Chunchon 200-701, South Korea.
Hwang
In Koo
IK
Koh
Un San
US
Lee
Jae Chul
JC
Kim
Dae Ho
DH
Kim
Jung Hwa
JH
Kim
Chang Ho
CH
Yoo
Ki-Yeon
KY
Kang
Tae-Cheon
TC
Hwang
Baek
B
Won
Moo Ho
MH
D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001369:Axons / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D004195:Disease Models, Animal; D005849:Gerbillinae; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism / Q000503:physiopathology; D009833:Olfactory Pathways / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
D016900:Neurofilament Proteins; C051774:neurofilament protein H