Direct conjugation of semiconductor nanoparticles with proteins.
Nanocrystalline CdS particles directly conjugated with bovine serum albumin (BSA) protein were prepared by applying the supercritical fluid processing technique, rapid expansion of a supercritical solution into a liquid solvent. The direct conjugation takes advantage of the unique features of the process for nanoparticle formation. The BSA-conjugated CdS nanoparticles in stable aqueous suspension or in the solid state were characterized by using microscopy, X-ray diffraction, and optical spectroscopy methods. The results show that well-dispersed CdS nanoparticles are coated with BSA in a core-shell-like arrangement and that the protein species associated with the nanoparticles remain functional according to the modified Lowry assay. These BSA-conjugated CdS nanoparticles are also strongly luminescent, with the luminescence spectrum contributed to primarily by the exciton emission.
Meziani
Mohammed J
MJ
Department of Chemistry, Howard L. Hunter Chemistry Laboratory, Clemson University, Clemson, South Carolina 29634-0973, USA.
Pathak
Pankaj
P
Harruff
Barbara A
BA
Hurezeanu
Razvan
R
Sun
Ya-Ping
YP
D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry*; D002417:Cattle; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012666:Semiconductors; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D014961:X-Ray Diffraction
D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D012710:Serum Albumin, Bovine