A novel replication-independent histone H2a gene in mouse.
BACKGROUND
An uncharacterized histone H2a-coding transcript (E130307C13) has been cloned from a mouse full-length cDNA library. This transcript is encoded on chromosome 6, approximately 4 kb upstream of a histone H4 gene, Hist4h4. The proteins encoded by this transcript and the human H2afj mRNA isoform-2 have the highest amino acid similarity. In this paper, we characterize it from the expression pattern given by quantitative RT-PCR.


RESULTS
Quantitative RT-PCR indicated that the gene that encodes E130307C13 (E130307C13) is regulated in a replication-independent manner, and therefore it is H2afj. Certainly, H2afj transcript lacks a stem-loop structure at the 3'-UTR but contains a poly (A) signal. In addition, its promoter region has a different structure from those of the replication-dependent histone H2a genes.


CONCLUSIONS
The bioinformatics imply that E130307C13 is a replication-independent H2a gene. In addition, quantitative RT-PCR analysis shows that it is replication-independent. Thus, it is H2afj, a novel replication-independent H2a gene in mouse.
Nishida
Hiromi
H
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. hnishida@gsc.riken.jp
Suzuki
Takahiro
T
Tomaru
Yasuhiro
Y
Hayashizaki
Yoshihide
Y
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D015723:Gene Library; D006657:Histones / Q000235:genetics*; D051379:Mice; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D006657:Histones; D012333:RNA, Messenger
Methods. 2001 Dec;25(4):402-8
11846609
Mol Cell Biol. 2002 Apr;22(7):2267-82
11884612
Genomics. 2002 Nov;80(5):487-98
12408966
Curr Opin Cell Biol. 2002 Dec;14(6):692-9
12473341
Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903
12477932
Nat Genet. 2004 Jan;36(1):40-5
14702039
Cell. 1987 Feb 27;48(4):615-26
3028643
Nucleic Acids Res. 1989 Nov 25;17(22):9113-26
2587254
Mol Cell Biol. 1991 May;11(5):2416-24
2017161
Annu Rev Biochem. 1991;60:827-61
1883210
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
Gene. 1999 Oct 18;239(1):1-14
10571029
Mol Cell. 2001 Apr;7(4):899-905
11336712
Bioinformatics. 2001 Dec;17(12):1244-5
11751241