title: Autologous gamete cryopreservation before hemopoietic stem cell transplantation.
abstract: BACKGROUND
  Infertility after hemopoietic stem cell transplantation (HST) is a serious problem for young patients. Autologous gamete collection before HST may be a promising strategy to overcome infertility.
  
  
  METHODS
  From October 1988 to December 2003, six male and nine female patients with hematological malignancies had autologous gametes collected before HST. The data on autologous gamete collection were analyzed.
  
  
  RESULTS
  Sperm could be collected from three patients. However, in two of the three, the numbers and motility of the sperm were severely depleted because they received chemotherapy for one and 11 cycles, respectively. Normal sperm was only collected from one patient with myelodysplastic syndrome who had no history of receiving chemotherapy. One or more oocytes could be collected in five of nine female patients, although the five received multiple cycles of chemotherapy. The successful oocyte collection was associated with an ovulation stimulant.
  
  
  CONCLUSIONS
  Autologous oocye collection before HST may be possible, even if patients receive multiple cycles of chemotherapy. In contrast, autologous sperm collection before HST may be difficult after patients receive chemotherapy. Successful pregnancy using autologous gametes after HST remains extremely difficult, especially in female patients; however, it is important to give information on infertility and autologous gamete collection to patients scheduled for HST.
authors.affiliation: Jichi Medical School, Minamikawachi, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagashima
authors.forename: Kazuo
authors.initials: K
authors.lastname: Muroi
authors.forename: Chizuru
authors.initials: C
authors.lastname: Kawano-Yamamoto
authors.forename: Takuji
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Ken
authors.initials: K
authors.lastname: Ohmine
authors.forename: Masaki
authors.initials: M
authors.lastname: Toshima
authors.forename: Akira
authors.initials: A
authors.lastname: Miyazato
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takatoku
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nagai
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Norio
authors.initials: N
authors.lastname: Komatsu
authors.forename: Keiya
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D015925:Cryopreservation; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000502:physiology*; D019337:Hematologic Neoplasms / Q000188:drug therapy; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007247:Infertility, Female / Q000209:etiology; D007248:Infertility, Male / Q000209:etiology; D007564:Japan; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D012189:Retrospective Studies; D013048:Specimen Handling / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D014182:Transplantation, Autologous