title: Differential activation of M26-containing meiotic recombination hot spots in Schizosaccharomyces pombe.
abstract: Certain genomic loci, termed hot spots, are predisposed to undergo genetic recombination during meiosis at higher levels relative to the rest of the genome. The factors that specify hot-spot potential are not well understood. The M26 hot spot of Schizosaccharomyces pombe is dependent on certain trans activators and a specific nucleotide sequence, which can function as a hot spot in a position- and orientation-independent fashion within ade6. In this report we demonstrate that a linear element (LE) component, Rec10, has a function that is required for activation of some, but not all, M26-containing hot spots and from this we propose that, with respect to hot-spot activity, there are three classes of M26-containing sequences. We demonstrate that the localized sequence context in which the M26 heptamer is embedded is a major factor governing whether or not this Rec10 function is required for full hot-spot activation. Furthermore, we show that the rec10-144 mutant, which is defective in full activation of ade6-M26, but proficient for activation of other M26-containing hot spots, is also defective in the formation of LEs, suggesting an intimate link between higher-order chromatin structure and local influences on hot-spot activation.
authors.affiliation: North West Cancer Research Fund Institute, University of Wales Bangor, UK.
authors.forename: David W
authors.initials: DW
authors.lastname: Pryce
authors.forename: Alexander
authors.initials: A
authors.lastname: Lorenz
authors.forename: Julia B
authors.initials: JB
authors.lastname: Smirnova
authors.forename: Josef
authors.initials: J
authors.lastname: Loidl
authors.forename: Ramsay J
authors.initials: RJ
authors.lastname: McFarlane
mesh_terms: D000483:Alleles; D008540:Meiosis / Q000235:genetics*; D009154:Mutation; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000502:physiology*; D013696:Temperature; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology
chemical_list: C096136:REC10 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators
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