title: Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis.
abstract: Candida albicans PALS-green fluorescent protein (GFP) reporter strains were inoculated into mice in a disseminated candidiasis model, and GFP production was monitored by immunohistochemistry and reverse transcription-PCR (RT-PCR). GFP production from the ALS1 and ALS3 promoters was detected immunohistochemically. ALS1, ALS2, ALS3, ALS4, and ALS9 transcription was detected by RT-PCR, further identifying ALS genes expressed in this model.
authors.affiliation: Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 61802, USA.
authors.forename: Clayton B
authors.initials: CB
authors.lastname: Green
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhao
authors.forename: Lois L
authors.initials: LL
authors.lastname: Hoyer
mesh_terms: D000373:Agglutinins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D016469:Fungemia / Q000382:microbiology*; D015870:Gene Expression; D005800:Genes, Fungal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C093538:ALS1 protein, Candida albicans; C113620:ALS3 protein, Candida albicans; D000373:Agglutinins; D005656:Fungal Proteins; D049452:Green Fluorescent Proteins
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