title: Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA.
abstract: Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method--Random Amplification of Polymorphic DNA (RAPD)--to identify genetic markers in B. anthracis strains. Twenty-five differential genetic markers were identified which divided the strains into five different groups. Three selected RAPD-markers were cloned and sequenced. The five RAPD-derived genotypes could be defined by integration of these three markers. This system offers a simple non-expensive method to classify B. anthracis strains in laboratories involved in the research of this bacterium.
authors.affiliation: Department of Infectious Diseases, Israel Institute for Biological Research, PO Box 19, Ness-Ziona, Israel. levy@iibr.gov.il
authors.forename: Haim
authors.initials: H
authors.lastname: Levy
authors.forename: Morly
authors.initials: M
authors.lastname: Fisher
authors.forename: Naomi
authors.initials: N
authors.lastname: Ariel
authors.forename: Zeev
authors.initials: Z
authors.lastname: Altboum
authors.forename: David
authors.initials: D
authors.lastname: Kobiler
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005819:Genetic Markers; D005838:Genotype; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers