[A research on the determination of trace secondary amines by reversed-phase high performance liquid chromatography with electrochemical detection].
A new sensitive method for determination of ultratrace secondary amines has been described. It is the first time to use dansyl chloride as electrochemical derivatization reagent for secondary amines. The optimum experimental conditions have been established. At first, trace secondary amines were derivatised with dansyl chloride at 40 degrees C for 30 minutes, then the mixture was injected and separated on a Perkin-Elmer/HS-3 C18 (83 mm x 4.6 mm i.d. 3 microm) column with methanol aqueous solution (70/30, pH 3.3) which contained 20 mmol/L potassium biphthalate buffer as mobile phase and detected with electrochemical detection at E = +1.1 V (Ag/AgCl). The calibration curves were linear in the range of 0.225-54 pg for dimethylamine; 1.1-264 pg for diethylamine, 0.43-102 pg for piperidine. The detection limits were 0.024, 0.60, 0.090 pg respectively and the coefficients of variation were less than 3.5% (n = 7).
Zhu
A
A
The Research Center of Physical and Chemical Analysis, Hebei University, Baoding, 071002.
Wang
Y
Y
Fu
C
C
D000588:Amines / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*
D000588:Amines