title: Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells.
abstract: We tested antiproliferative activity of selected cruciferous phytoalexins including brassinin, 1-methoxybrassinin, (+/-)-spirobrassin, (+/-)-1-methoxyspirobrassinin and (+/-)-1-methoxyspirobrassinol, in leukemic Jurkat cell. The most effective of the tested phytoalexins was 1-methoxybrassinin with IC(50) 10 micromol l(-1). However, significant effect of all phytoalexines was also determined at concentration 1 micromol l(-1). In 1-methoxybrassinin-treated Jurkat cells, we found significant increase in the fraction of cells with a sub-G(0)/G(1) DNA content, which is considered to be a marker of cell death by apoptosis. Apoptosis was also confirmed by the annexin V staining. In summary, 1-methoxybrassinin exerted potent antiproliferative activity probably due to cell cycle arrest and apoptosis induction.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, P.J. Safárik University, Kosice, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Pilátová
authors.forename: M
authors.initials: M
authors.lastname: Sarisský
authors.forename: P
authors.initials: P
authors.lastname: Kutschy
authors.forename: A
authors.initials: A
authors.lastname: Mirossay
authors.forename: R
authors.initials: R
authors.lastname: Mezencev
authors.forename: Z
authors.initials: Z
authors.lastname: Curillová
authors.forename: M
authors.initials: M
authors.lastname: Suchý
authors.forename: K
authors.initials: K
authors.lastname: Monde
authors.forename: L
authors.initials: L
authors.lastname: Mirossay
authors.forename: J
authors.initials: J
authors.lastname: Mojzis
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D019607:Brassicaceae; D016923:Cell Death / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000633:toxicity*; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins
chemical_list: D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins