The effect of basic fibroblast growth factor on delayed-replanted monkey teeth.
BACKGROUND
Basic fibroblast growth factor (bFGF; FGF-2) has been reported to facilitate wound healing and periodontal regeneration in experimental alveolar bone defects. The purpose of this study was to evaluate histologically the effect of topically applied bFGF with or without fibrin glue on delayed-replanted monkey teeth prone to replacement resorption.


METHODS
Forty-five roots from five monkeys were endodontically treated aseptically and then extracted as atraumatically as possible. Ten negative control roots were replanted immediately, while 12 positive control roots were allowed to bench dry for 1 hour prior to replantation, both without further treatment. Roots in the two experimental groups were bench dried for 1 hour, rinsed with saline, and then replanted into sockets filled with bFGF with (11 roots) or without (12 roots) fibrin glue. After 12 weeks, histological sections were prepared and evaluated according to morphometric analysis as complete healing or unfavorable healing composed of inflammatory resorption and replacement resorption.


RESULTS
Kruskal-Wallis and Mann-Whitney U tests showed teeth in the negative control group to have significantly higher complete healing (98.88% +/- 2.30%) and significantly lower unfavorable healing (1.12% +/- 2.30%) than the positive control group and the experimental groups. bFGF/fibrin glue group showed higher occurrence of complete healing (39.06% +/- 41.62%) compared to the bFGF group (25.28% +/- 28.85%) and the positive control group (16.58% +/- 19.60%), although the differences were not significant. Comparing the complete and unfavorable healing, there was no significant difference in the bFGF/fibrin glue group (P = 0.47), but the differences were significant in the other groups (P < 0.05).


CONCLUSIONS
Topical application of bFGF with fibrin glue showed an insignificantly higher occurrence of complete healing in delayed-replanted monkey teeth.
Sae-Lim
Varawan
V
Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, Republic of Singapore. rsdvsl@nus.edu.sg
Ong
Wei Yi
WY
Li
Zhimei
Z
Neo
Jennifer
J
D000818:Animals; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008252:Macaca fascicularis; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D011897:Random Allocation; D012391:Root Resorption / Q000517:prevention & control*; D018709:Statistics, Nonparametric; D013997:Time Factors; D014014:Tissue Adhesives / Q000494:pharmacology; D014090:Tooth Replantation; D014092:Tooth Root / Q000187:drug effects*; D020390:Tooth Socket / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects
D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D016222:Fibroblast Growth Factor 2