Analytical relationships among Biosite, Bayer, and Roche methods for BNP and NT-proBNP.
This study determined whether, for patient monitoring, it is feasible to convert B-type natriuretic peptide (BNP) results obtained using Triage (BNP, Biosite, San Diego, CA), Centaur (BNP, Bayer Diagnostics, Tarrytown, NY), and Elecsys 2010 (N-terminal proBNP; Roche, Indianapolis, IN) assays. Concordance between assays and effects of renal impairment also were assessed. Samples were primarily from emergency center patients. Biosite testing was performed immediately; Bayer and Roche testing was performed later on plasma stored frozen (-30 degrees C). Logistic regression relationships were as follows: Bayer = 0.57 Biosite + 23.1, n = 121, R2 = 0.85; Roche = 6.09 Biosite -220.4 + 1,131.6 (if female), n = 131, R2 = 0.57; and Roche = 15.34 Bayer + 2,400.8, n = 150, R2 = 0.23. An increased serum creatinine level (>/=2 mg/dL [>/=177 micromol/L]) influenced the Roche results. We conclude the following from this preliminary study: (1) Results from one method cannot be converted reliably to another using regression relationships. (2) When using manufacturers'cutoff values, concordance between assays was acceptable. (3) Renal impairment affected Roche results.
Sykes
Elizabeth
E
Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, MI 48073, USA.
Karcher
Raymond E
RE
Eisenstadt
Janice
J
Tushman
David A
DA
Balasubramaniam
Mamtha
M
Gusway
John
J
Perason
Valerie J
VJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001322:Autoanalysis; D015415:Biomarkers / Q000032:analysis; D002624:Chemistry, Clinical / Q000379:methods; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000175:diagnosis; D006801:Humans; D008297:Male; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain; D045265:Natriuretic Peptides / Q000097:blood*; D009419:Nerve Tissue Proteins / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D015203:Reproducibility of Results
D015415:Biomarkers; D045265:Natriuretic Peptides; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain