TOM software toolbox: acquisition and analysis for electron tomography.
Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. "TOM software toolbox" integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.
Nickell
Stephan
S
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany. Nickell@biochem.mpg.de
Förster
Friedrich
F
Linaroudis
Alexandros
A
Net
William Del
WD
Beck
Florian
F
Hegerl
Reiner
R
Baumeister
Wolfgang
W
Plitzko
Jürgen M
JM
D000465:Algorithms; D006801:Humans; D007091:Image Processing, Computer-Assisted; D012984:Software; D014054:Tomography / Q000295:instrumentation / Q000379:methods*; D014584:User-Computer Interface