title: [Determination of gallic acid in Melastoma dodecandrum by RP-HPLC].
abstract: OBJECTIVE
  To establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb.
  
  
  METHODS
  The RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm.
  
  
  RESULTS
  The content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations.
  
  
  CONCLUSIONS
  The method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum.
authors.affiliation: School of Pharmacy, Guiyang Medical College, Guiyang 550004, China. hexun224@gmc.edu.cn
authors.forename: Xun
authors.initials: X
authors.lastname: He
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Li-na
authors.initials: LN
authors.lastname: Liu
authors.forename: Yan-yu
authors.initials: YY
authors.lastname: Lan
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Wang
authors.forename: Yong-lin
authors.initials: YL
authors.lastname: Wang
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D005707:Gallic Acid / Q000032:analysis*; D035322:Melastomataceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D005707:Gallic Acid