title: Continuous transforming growth factor beta1 secretion by cell-mediated gene therapy maintains chondrocyte redifferentiation.
abstract: One of the most important factors in the production of cartilage is transforming growth factor beta1 (TGF-beta1). To obtain sustained release of TGF-beta1, a cell-mediated gene therapy technique was introduced. We infected chondrocytes with a retroviral vector carrying the TGF-beta1 gene. The single clone derivative showed sustained TGF-beta1 secretion. It also showed constitutive type II collagen expression. Whereas the TGF-beta1 protein itself is unable to induce formation of cartilage in vivo, human chondrocytes engineered to express a retroviral vector encoding TGF-beta1 showed cartilage formation in vivo when cells were injected into nude mice intradermally. These data suggest that cell-mediated gene therapy using TGF-beta1 as a transgene would be a promising treatment for osteoarthritis.
authors.affiliation: TissueGene, Gaithersburg, Maryland 20877, USA. dklee@tissuegene.com
authors.forename: Dug Keun
authors.initials: DK
authors.lastname: Lee
authors.forename: Kyoung Baek
authors.initials: KB
authors.lastname: Choi
authors.forename: In Suk
authors.initials: IS
authors.lastname: Oh
authors.forename: Sun U
authors.initials: SU
authors.lastname: Song
authors.forename: Sally
authors.initials: S
authors.lastname: Hwang
authors.forename: Chae-Lyul
authors.initials: CL
authors.lastname: Lim
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Hyun
authors.forename: Hyeon-Youl
authors.initials: HY
authors.lastname: Lee
authors.forename: Guang Fan
authors.initials: GF
authors.lastname: Chi
authors.forename: Youngsuk
authors.initials: Y
authors.lastname: Yi
authors.forename: Vivian
authors.initials: V
authors.lastname: Yip
authors.forename: Jeannie
authors.initials: J
authors.lastname: Kim
authors.forename: Eun Byul
authors.initials: EB
authors.lastname: Lee
authors.forename: Moon Jong
authors.initials: MJ
authors.lastname: Noh
authors.forename: Kwan Hee
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation; D017690:Cell Transplantation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000637:transplantation; D024043:Collagen Type II / Q000187:drug effects / Q000378:metabolism; D005240:Feasibility Studies; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D012190:Retroviridae / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D053773:Transforming Growth Factor beta1; D019076:Transgenes; D014183:Transplantation, Heterologous
chemical_list: D024043:Collagen Type II; C508960:TGFB1 protein, human; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1