title: Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization.
abstract: Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d(3)-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.
authors.affiliation: College of Pharmacy, Division of Clinical and Administrative Pharmacy, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Schmidt
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Prisinzano
authors.forename: Kevin
authors.initials: K
authors.lastname: Tidgewell
authors.forename: Wayne
authors.initials: W
authors.lastname: Harding
authors.forename: Eduardo R
authors.initials: ER
authors.lastname: Butelman
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Kreek
authors.forename: Daryl J
authors.initials: DJ
authors.lastname: Murry
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D045785:Diterpenes, Clerodane; D006213:Hallucinogens / Q000032:analysis; D006801:Humans; D008253:Macaca mulatta; D017473:Receptors, Opioid, kappa / Q000819:agonists; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004224:Diterpenes; D045785:Diterpenes, Clerodane; D006213:Hallucinogens; D017473:Receptors, Opioid, kappa; C090499:salvinorin A