The metal permease ZupT from Escherichia coli is a transporter with a broad substrate spectrum.
The Escherichia coli zupT (formerly ygiE) gene encodes a cytoplasmic membrane protein (ZupT) related to members of the eukaryotic ZIP family of divalent metal ion transporters. Previously, ZupT was shown to be responsible for uptake of zinc. In this study, we show that ZupT is a divalent metal cation transporter of broad substrate specificity. An E. coli strain with a disruption in all known iron uptake systems could grow in the presence of chelators only if zupT was expressed. Heterologous expression of Arabidopsis thaliana ZIP1 could also alleviate iron deficiency in this E. coli strain, as could expression of indigenous mntH or feoABC. Transport studies with intact cells showed that ZupT facilitates uptake of 55Fe2+ similarly to uptake of MntH or Feo. Other divalent cations were also taken up by ZupT, as shown using 57Co2+. Expression of zupT rendered E. coli cells hypersensitive to Co2+ and sensitive to Mn2+. ZupT did not appear to be metal regulated: expression of a Phi(zupT-lacZ) operon fusion indicated that zupT is expressed constitutively at a low level.
Grass
Gregor
G
Institut für Mikrobiologie, Martin-Luther-Universität, Halle, Germany.
Franke
Sylvia
S
Taudte
Nadine
N
Nies
Dietrich H
DH
Kucharski
Lisa M
LM
Maguire
Michael E
ME
Rensing
Christopher
C
D003035:Cobalt / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D019216:Metals, Heavy / Q000378:metabolism*; D009154:Mutation; D013379:Substrate Specificity / Q000502:physiology; D015032:Zinc / Q000378:metabolism
D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D019216:Metals, Heavy; C497880:ZupT protein, E coli; D003035:Cobalt; D008345:Manganese; D007501:Iron; D015032:Zinc
J Biol Inorg Chem. 1999 Oct;4(5):523-7
10550680
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5624-8
8643627
J Membr Biol. 1997 Mar 15;156(2):99-103
9075641
J Bacteriol. 1997 Oct;179(19):6201-4
9324273
J Bacteriol. 1997 Oct;179(20):6228-37
9335267
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7220-4
9618566
Mol Microbiol. 1998 Jun;28(6):1199-210
9680209
J Bacteriol. 2004 Nov;186(22):7499-507
15516561
Science. 2004 Nov 5;306(5698):1025-8
15459345
Arch Microbiol. 2005 Jan;183(1):9-18
15549269
J Biol Chem. 2000 Feb 25;275(8):5560-4
10681536
Mol Microbiol. 2000 Mar;35(5):1065-78
10712688
Biochim Biophys Acta. 2000 May 1;1465(1-2):190-8
10748254
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
10829079
J Biol Chem. 2001 Jun 22;276(25):22258-64
11301334
Microbiology. 2001 Jul;147(Pt 7):1709-18
11429449
J Bacteriol. 2001 Aug;183(15):4664-7
11443104
J Bacteriol. 2002 Feb;184(3):864-6
11790762
Mol Microbiol. 2002 Apr;44(2):561-71
11972791
Plant Cell. 2002 Jun;14(6):1347-57
12084831
Infect Immun. 2002 Nov;70(11):6032-42
12379679
FEMS Microbiol Lett. 2002 Oct 8;215(2):273-8
12399046
Infect Immun. 2003 May;71(5):2656-64
12704140
Infect Immun. 2004 Oct;72(10):5858-67
15385487
J Bacteriol. 1976 Jun;126(3):1096-103
780341
Eur J Biochem. 1981 Jul;117(2):431-7
6268411
J Bacteriol. 1985 Apr;162(1):328-34
3884593
Anal Biochem. 1987 Jan;160(1):47-56
2952030
J Bacteriol. 1989 Feb;171(2):775-83
2536681
J Bacteriol. 1992 Dec;174(24):8102-10
1459958
J Bacteriol. 1993 Oct;175(19):6212-9
8407793
J Bacteriol. 1999 Nov;181(22):6876-81
10559151