title: A molecularly imprinted polymer on indium tin oxide and silicon.
abstract: Molecular imprinting is a technique for creating artificial receptor sites in a polymer. Molecularly imprinted polymers (MIPs) are produced by forming a polymer around a molecule that is used as the template. Upon removal of the template, molecular holes remain which are specific in shape and size to the target molecule. In this research, a MIP was formed for theophylline using a copolymer of methacrylic acid and ethylene glycol dimethacrylate. The theophylline MIP was formed on two platforms: indium tin oxide (ITO) and silicon, which were used as the working electrode for cyclic voltammetry measurements. The presence of theophylline was measured using cyclic voltammetry and corresponded to the peak current on the cyclic voltammograms. The results of this research agreed with previous results of MIPs immobilized on an ITO platform. The peak currents of the MIP in the presence and absence of theophylline were compared to the blank polymer for each platform. The ratio of peak currents on ITO increased by a factor of 9.5 for the MIP compared to the non-imprinted polymer. Similarly, the ratio of peak currents on silicon increased by a factor of 6 compared to the non-imprinted polymer. This research demonstrated a procedure for evaluating a MIP layer on two different platforms.
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, 204 Farrall Hall, East Lansing, MI 48824, USA. kindschl@msu.edu
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Kindschy
authors.forename: Evangelyn C
authors.initials: EC
authors.lastname: Alocilja
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D025941:Protein Interaction Mapping / Q000295:instrumentation / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D011108:Polymers; D017971:Tin Compounds; C109984:indium tin oxide; D012825:Silicon