title: Translation enhancement by optimized downstream box sequences in Escherichia coli and Mycobacterium smegmatis.
abstract: The effect on translation of downstream box sequences optimized for binding to Mycobacterium smegmatis and Escherichia coli 16S rRNA in the absence of a Shine-Dalgarno (SD) region was investigated. The relative translational efficiency of each construct in either M. smegmatis or E. coli was determined. Eradication of the SD region in the absence of a downstream box abolished the translation activity. In contrast, optimized downstream box constructs resulted in a 13- and 18-fold increase in protein synthesis, relative to non-optimized DB controls in E. coli and M. smegmatis, respectively.
authors.affiliation: Institute of Infectious Diseases and Molecular Medicine, Health Sciences Faculty, University of Cape Town, Observatory, 7925, Cape Town, South Africa. grush@curie.uct.ac.za
authors.forename: Gavin J
authors.initials: GJ
authors.lastname: Rush
authors.forename: Lafras M
authors.initials: LM
authors.lastname: Steyn
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D012336:RNA, Ribosomal, 16S