title: Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR.
abstract: Multiplex PCR was established to detect porcine circovirus type 2 (PCV-2), porcine parvovirus (PPV) and porcine pseudorabies virus (PRV) and applied to samples from 137 piglets exhibiting clinical signs of postweaning multisystemic wasting syndrome (PMWS). PCV-2 DNA was detected from all samples. Moreover, 43 samples were positive for PPV but negative for PRV; 11 samples were positive for PRV but negative for PPV; and 35 samples were positive both for PPV and PRV. These results suggests that PCV-2 co-infection with PRV and PPV may play an important role in PMWS. Also, multiplex PCR is an appropriate candidate method for diagnosis of PCV-2, PRV and PPV simultaneously in field cases.
authors.affiliation: Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China.
authors.forename: S
authors.initials: S
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Lü
authors.forename: S
authors.initials: S
authors.lastname: Xiao
authors.forename: M
authors.initials: M
authors.lastname: Jin
authors.forename: A
authors.initials: A
authors.lastname: Guo
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: He
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000302:isolation & purification; D008168:Lung / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011557:Pseudorabies / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D019282:Wasting Syndrome / Q000662:veterinary* / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral