A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species.
A PCR-RFLP method targeted toward 26S rDNA and with 2 restriction enzymes, CfoI and BstF51, was developed to identify 11 Malassezia species. Not only type and standard strains but also 13 clinical isolates were identified successfully in this study. The results of identifications were confirmed by DNA sequencing.
Mirhendi
Hossein
H
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, PO Box 14155-6446, Tehran, Iran. mirhendi@yahoo.com
Makimura
Koichi
K
Zomorodian
Kamiar
K
Yamada
Tsuyoshi
T
Sugita
Takashi
T
Yamaguchi
Hideyo
H
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D003881:Dermatomycoses / Q000382:microbiology*; D006801:Humans; D008290:Malassezia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D013045:Species Specificity
D004271:DNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S