Oxidative stress-related gene polymorphism and the risk of preeclampsia.
OBJECTIVE
The purpose of this study was to determine whether genetic variability in oxidative stress-related enzymes contributes to individual preeclampsia susceptibility differences.


METHODS
Polymorphisms in the cytochrome P450 (CYP)1A1 (MspI), CYP1A1(Ile/Val), glutathione S-transferase (GST)M1, GSTT1, myeloperoxidase (MPO), and manganese superoxide dismutase (MnSOD) genes were evaluated by polymerase chain reaction (PCR) or PCR-restriction fragment length polymorphism (RFLP) in 214 healthy controls with an uncomplicated obstetric history, and in 121 preeclampsia patients. Chi2 analyses were used to statistically evaluate differences.


RESULTS
No significant differences in the CYP1A1(MspI) or CYP1A1(Ile/Val) genotypes were observed between the healthy controls and the preeclampsia patients (chi2 = 1.43, P = 0.49 versus chi2 = 1.54, P = 0.46). The GSTM1 homozygous null type and GSTT1 homozygous null type were no differences in the patients and controls (chi2 = 0.01, P = 0.92 versus chi(2) = 0.31, P = 0.57), and no significant differences in the polymorphisms of the MPO and MnSOD genotypes were found between the patients and controls (chi2 = 2.00, P = 0.37 versus chi2 = 0.07, P = 0.96).


CONCLUSIONS
Polymorphisms in the oxidative stress-related genes (CYP1A1, GSTM1, GSTT1, MPO, MnSOD) do not seem to be risk factors for preeclampsia.
Kim
Young Ju
YJ
Department of Obstetrics and Gynecology, Ewha Womans University, 911-1 MokDong Yangcheonku, Seoul 151-070, Korea. kkyj@ewha.ac.kr
Park
Hye Sook
HS
Park
Mi Hye
MH
Suh
Suk Hyo
SH
Pang
Myung-Geol
MG
D000328:Adult; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D018384:Oxidative Stress / Q000235:genetics; D009195:Peroxidase / Q000235:genetics*; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000235:genetics*
D009195:Peroxidase; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D005982:Glutathione Transferase