title: Opposing roles for Drosophila JAK/STAT signalling during cellular proliferation.
abstract: The JAK/STAT signalling pathway mediates both antiproliferative responses following interferon stimulation and cellular proliferation in response to cytokines such as interleukins and growth factors. Central to these responses are the seven vertebrate STAT molecules, misregulation of which is implicated in a variety of malignancies. We have investigated the proliferative role of the single Drosophila STAT92E, part of the evolutionarily conserved JAK/STAT cascade. During second instar larval wing disc development pathway activity is both necessary and sufficient to promote proliferation of this epithelial cell type. However by later stages, endogenous STAT92E is stimulated by a noncannonical mechanism to exert pronounced antiproliferative effects. Ectopic canonical activation is sufficient to further decrease proliferation and leads to the premature arrest of cells in the G2 phase of the cell cycle. The single STAT92E present in Drosophila therefore mediates both proproliferative functions analogous to vertebrate interleukin-stimulated STAT3 and antiproliferative functions analogous to interferon-stimulated STAT1. Pro- and antiproliferative roles therefore represent ancestral activities conserved through evolution and subsequently assigned to distinct molecules.
authors.affiliation: Department of Molecular Developmental Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Mukherjee
authors.forename: James Castelli-Gair
authors.initials: JC
authors.lastname: Hombría
authors.forename: Martin P
authors.initials: MP
authors.lastname: Zeidler
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D004330:Drosophila / Q000254:growth & development* / Q000502:physiology*; D029721:Drosophila Proteins / Q000494:pharmacology*; D053612:Janus Kinases; D007814:Larva / Q000235:genetics / Q000254:growth & development; D011505:Protein-Tyrosine Kinases / Q000494:pharmacology*; D050791:STAT Transcription Factors; D015398:Signal Transduction; D015534:Trans-Activators / Q000494:pharmacology*; D014157:Transcription Factors / Q000494:pharmacology*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D050791:STAT Transcription Factors; C105675:Stat92E protein, Drosophila; D015534:Trans-Activators; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases; D053612:Janus Kinases; C085845:hop protein, Drosophila