Sprouting from arteriovenous shunt vessels with increased blood flow.
The purpose of this study was to evaluate the effect of increased blood flow on angiogenesis at the large vessels. The arteriovenous (AV) shunt was made on the thigh of male Wistar rats (n = 27) to increase blood flow, wrapped with artificial skin dermis, which consisted of a silicon outer layer, and isolated from surrounding tissues. Blood flow increased from 2.40 +/- 0.77 to 35.8 +/- 8.7 ml min(-1) (14.9 times), and the shear stress index (relative value of shear stress) increased from 10.7 +/- 3.6 to 73.4 +/- 18.1 (6.85 times) 60 min after the shunt formation. Newly formed vessels were observed around the AV shunt loop. Scanning electron micrographs at the AV shunt vessel lumen showed modified endothelial cells at day 7 and a remarkable number of pores at day 14. The volume of newly formed vessels was increased 12 times from day 5 to day 14. The mechanical factor of shear stress was considered the major stimulator of angiogenesis. This is the first report of electron-microscopic observation of sprouts from a large vessel lumen. The new AV shunt model is useful for basic research on angiogenesis at the large vessels in vivo and, furthermore, could generate vascularised tissues with various cultured cells.
Asano
Y
Y
Department of Plastic Surgery, University of Tokyo, Japan.
Ichioka
S
S
Shibata
M
M
Ando
J
J
Nakatsuka
T
T
D000818:Animals; D001166:Arteriovenous Shunt, Surgical; D004730:Endothelium, Vascular / Q000648:ultrastructure; D005263:Femoral Artery / Q000601:surgery; D005268:Femoral Vein / Q000601:surgery; D018056:Hemorheology; D008297:Male; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow
J Biomech Eng. 1981 Aug;103(3):177-85
7278196
J Biomech Eng. 1986 Nov;108(4):338-41
3795879
Br J Plast Surg. 2000 Jan;53(1):51-7
10657450
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(2):127-39
2467435
Clin Plast Surg. 2003 Oct;30(4):485-98
14621297
Am J Physiol. 1980 Jul;239(1):H14-21
7396013
Arterioscler Thromb Vasc Biol. 2003 Jul 1;23 (7):1143-51
12676799
Plast Reconstr Surg. 2003 Nov;112(6):1636-44
14578795
Circ Res. 1972 Jan;30(1):23-33
5007525
Kaibogaku Zasshi. 1994 Oct;69(5):684-96
7531382
Plast Reconstr Surg. 1991 Mar;87(3):509-17
1705352
Nat Med. 2000 Apr;6(4):389-95
10742145
J Appl Physiol (1985). 1998 Dec;85(6):2025-32
9843522
Arteriosclerosis. 1986 Mar-Apr;6(2):146-54
3954669
Microcirculation. 2002 Apr;9(2):111-24
11932778
J Surg Res. 1997 Sep;72(1):29-35
9344711
Tissue Eng. 2000 Dec;6(6):595-603
11103081
Cardiovasc Res. 1989 Nov;23(11):913-20
2611800
Science. 1986 Jan 24;231(4736):405-7
3941904
J Submicrosc Cytol Pathol. 1989 Jan;21(1):1-34
2702606
Physiol Rev. 1992 Apr;72(2):369-417
1372998
Plast Reconstr Surg. 1980 Jul;66(1):109-15
6156468
Nat Med. 2003 Jun;9(6):653-60
12778163
J Biomech Eng. 1986 Nov;108(4):332-7
3795878