title: Experimental prediction of the evolution of ceftazidime resistance in the CTX-M-2 extended-spectrum beta-lactamase.
abstract: We applied in vitro evolution to an Escherichia coli strain containing bla(CTX-M-2) and obtained 10 independent mutant bla(CTX-M-2) alleles that confer elevated resistance to ceftazidime (MIC > or = 32 microg/ml) but lost the ability to confer resistance to cefepime. All alleles had a Pro-to-Ser substitution at position 167.
authors.affiliation: Department of Epidemiology, Rollins School of Public Health, Emory University, 1518 Clifton Rd., Atlanta, GA 30322, USA.
authors.forename: Kerry J
authors.initials: KJ
authors.lastname: Welsh
authors.forename: Miriam
authors.initials: M
authors.lastname: Barlow
authors.forename: Fred C
authors.initials: FC
authors.lastname: Tenover
authors.forename: James W
authors.initials: JW
authors.lastname: Biddle
authors.forename: J Kamile
authors.initials: JK
authors.lastname: Rasheed
authors.forename: Leigh Ann
authors.initials: LA
authors.lastname: Clark
authors.forename: John E
authors.initials: JE
authors.lastname: McGowan
mesh_terms: D000483:Alleles; D002442:Ceftazidime / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D008826:Microbial Sensitivity Tests; D009154:Mutation; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D002442:Ceftazidime; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases
references.citation: Int J Antimicrob Agents. 2000 Mar;14(2):137-42
references.pmid: 10720804
references.citation: Clin Infect Dis. 2001 Apr 1;32(7):1085-9
references.pmid: 11264037
references.citation: Antimicrob Agents Chemother. 2001 Dec;45(12):3355-61
references.pmid: 11709308
references.citation: Genetics. 2002 Mar;160(3):823-32
references.pmid: 11901104
references.citation: Emerg Infect Dis. 2002 May;8(5):522-4
references.pmid: 11996691
references.citation: J Antimicrob Chemother. 2002 Dec;50(6):1031-4
references.pmid: 12461028
references.citation: Biochem J. 1991 May 15;276 ( Pt 1):269-70
references.pmid: 2039479
references.citation: Genetics. 2003 May;164(1):23-9
references.pmid: 12750318
references.citation: Antimicrob Agents Chemother. 2003 Sep;47(9):2864-7
references.pmid: 12936986
references.citation: Antimicrob Agents Chemother. 2004 Jan;48(1):1-14
references.pmid: 14693512
references.citation: J Clin Microbiol. 2004 Sep;42(9):3978-84
references.pmid: 15364979
references.citation: J Gen Microbiol. 1976 May;94(1):55-67
references.pmid: 819625
references.citation: J Antimicrob Chemother. 1989 Jul;24(1):82-4
references.pmid: 2789212
references.citation: Genetics. 2003 Apr;163(4):1237-41
references.pmid: 12702671