title: [Effect of Shenluotong on secretion of ECM and expression of TGF-beta1 in rats mesangial cells of rats].
abstract: OBJECTIVE
  To investigate the effect of Shenluotong on the expression of transforming growth factor-beta1 (TGF-beta1) and extracellular matrix (ECM) in Ang II-induced MCs.
  
  
  METHODS
  Fibronectin (FN) and collagen type IV (Col IV) of extracellular matrix were detected by enzyme-linked immunosorbent assay; the expression of TGF-beta1 mRNA were measured by semi-quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  A positive correlation between TGF-beta1 and ECM were found in the present study. FN, Col IV and TGF-beta1 mRNA were inhibited by Shenluotong significantly.
  
  
  CONCLUSIONS
  Shenluotong can decrease the accumulation of ECM and inhibit the expression of TGF-beta1, suggesting further that shenluotong can be used to prevent and treat various glomerular diseases and delay glomerular sclerosis.
authors.affiliation: Traditional Chinese Medicine college, Hebei Medical University, shijiazhuang 050091, China.
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Wang
authors.forename: Yu-yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Qing-you
authors.initials: QY
authors.lastname: Xu
authors.forename: Hua-zhou
authors.initials: HZ
authors.lastname: Xu
authors.forename: Tong-zhong
authors.initials: TZ
authors.lastname: Li
authors.forename: Xian-ming
authors.initials: XM
authors.lastname: Hou
mesh_terms: D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D005920:Glomerular Mesangium / Q000166:cytology*; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D009835:Oligochaeta / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1
chemical_list: D024141:Collagen Type IV; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D008419:Materia Medica; D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1