title: Comparative study on schizontocidal activity of recrystallized or crude daphnetin against malaria parasites.
abstract: OBJECTIVE
  To compare the schizontocidal activity of recrystallized or crude daphnetin against malaria parasites in vivo.
  
  
  METHODS
  Schizontocidal activity of recrystallized or crude daphnetin at various dosages was assessed in mice infected with Plasmodium berghei ANKA using a "4-day suppress assay".
  
  
  RESULTS
  The comparison of the reduction rate of parasitemia caused by either recrystallized or crude dephnetin showed that ED(50) of crude daphnetin was 18.36 mg/kg, with 95% confidence limit of 5.96-56.54 mg/kg while ED50 of recrystallized daphnetin was 11.46 mg/kg, with 95% confidence limit of 8.63-15.22 mg/kg.
  
  
  CONCLUSIONS
  The results indicate that the efficacy of recrystallized daphnetin is 37.6% higher than that of crude daphnetin.
authors.affiliation: Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China. wangqinmei@hotmail.com
authors.forename: Qin-Mei
authors.initials: QM
authors.lastname: Wang
authors.forename: Yi-Chang
authors.initials: YC
authors.lastname: Ni
authors.forename: Jian
authors.initials: J
authors.lastname: Guo
authors.forename: Jia-Tong
authors.initials: JT
authors.lastname: Wu
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Qian
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology*; D051379:Mice; D021261:Parasitic Sensitivity Tests; D010962:Plasmodium berghei / Q000187:drug effects*; D014468:Umbelliferones / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D014468:Umbelliferones; C039952:daphnetin