title: Preparation and in vitro antioxidant activity of kappa-carrageenan oligosaccharides and their oversulfated, acetylated, and phosphorylated derivatives.
abstract: In order to study the relationship between chemical structure and properties of modified carrageenans versus antioxidant activity in vitro, kappa-carrageenan oligosaccharides were prepared through mild hydrochloric acid hydrolysis of the polysaccharide, and these were used as starting materials for the partial synthesis of their oversulfated, acetylated, and phosphorylated derivatives. The structure and substitution pattern of the oligosaccharides and their derivatives were studied using FTIR and (13)C NMR spectroscopy, and their in vitro antioxidant activities were investigated. Certain derivatives of the carrageenan oligosaccharides exhibited higher antioxidant activity than the polysaccharides and oligosaccharides in certain antioxidant systems. The oversulfated and acetylated derivatives, which scavenge superoxide radicals, the phosphorylated and low-DS acetylated derivatives, which scavenge hydroxyl radicals, and the phosphorylated derivatives, which scavenge DPPH radicals, all exhibited significant antioxidant activities in the systems examined. The effect of the molecular weight of the carrageenan on antioxidant activities, however, is not obvious from these studies.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Huamao
authors.initials: H
authors.lastname: Yuan
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xuegang
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Lü
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xuelu
authors.initials: X
authors.lastname: Gao
authors.forename: Jinming
authors.initials: J
authors.lastname: Song
mesh_terms: D000107:Acetylation; D000975:Antioxidants / Q000737:chemistry*; D002351:Carrageenan / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D066298:In Vitro Techniques; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D010766:Phosphorylation; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D002351:Carrageenan