[Effects on the survival rates and damage on DNA of benzo[a]pyrene or lead alone or in combination in rat neurons in vivo].
OBJECTIVE
To study the effects on the survival rates and DNA damage of benzo[a]pyrene or lead alone or in combination in rat neurons in vivo.


METHODS
Primary cultures of rat cerebellar neurons were prepared from 8-day-old pups, 10 groups cultures in each experiments were used, (1) control group, (2) vehicle group (Equal volume of DMSO + S9-mix), (3) low dose lead group (PbAc 5 micromol/L), (4) high dose lead group (PbAc 50 micromol/L), (5) low dose BaP group (BaP 5 micromol/L+ S9-mix), (6) high dose BaP group (BaP 50 micromol/L + S9-mix), (7) low dose lead + low dose BaP group, (8) low dose lead + high dose BaP group, (9) high dose lead + low dose BaP group, (10) high dose lead + high dose BaP group. After 90 min administration, the neurons were collected by the method of trypsin digestion, the survival rates were detected by the method of Tapian-blue dying, Detect cracks on the DNA straps by the method of SCGE.


RESULTS
(1) The survival rates of all the administrated groups were significantly lower than that of control groups (P < 0.05, P < 0.01). (2) In the high-dose BaP group (group 6), groups combine administrated with BaP and lead, one or two of them at high dose (groups 8, 9, 10), the DNA damage were more serious than that of control groups (P < 0.01).


CONCLUSIONS
(1) Both BaP and lead with some vivo neurotoxicity, the effects of two toxicants can do concord. (2) Neuron DNA damage may be one of the mechanisms by which BaP result in toxicity on the cultured neurons.
Tu
Baijie
B
Department of Occupational/Environmental Medicine, Chongqing University of Medical Science, Chongqing 400016, China.
Cheng
Shuqun
S
Hu
Xueyuan
X
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D007854:Lead / Q000633:toxicity*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D007854:Lead; D001564:Benzo(a)pyrene