[Protein changes in human embryonic lung fibroblasts after hydroquinone stimulation using proteomic technique].
OBJECTIVE
To explore the molecular mechanism of cell response induced by hydroquinone, we investigated the protein profile after treatment with hydroquinone in human embryonic lung fibroblasts.


METHODS
After HQ treatment, cells were lysed in lysis solution, then the supernatant were collected and precipitated with acetone prior to protein concentration determination. The total cellular proteins were separated using two-dimensional gel electrophoresis and visualized by colloidal coomassie blue staining. Digital images were analyzed using Imagemaster 2.0 software. The differentially expressed protein spots were picked and digestion in gel then identified by peptide mass fingerprinting using MALDI-TOF.


RESULTS
There were 15 protein spots changed after HQ stimulation. Among them 8 protein spots were identified by PMF including some oxidative stress and cytoskeleton related proteins.


CONCLUSIONS
Protein profile was altered after HQ stimulation.
Li
Xiyi
X
School of Public Health, Zhongshan University, Guangzhou 510080, China.
Zhuang
Zhixiong
Z
Liu
Jianjun
J
Huang
Haiyan
H
Wei
Qinzhi
Q
Yang
Xiaohua
X
D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006873:Hydroquinones / Q000633:toxicity*; D008168:Lung / Q000166:cytology / Q000196:embryology; D009153:Mutagens / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006873:Hydroquinones; D009153:Mutagens; D011506:Proteins; C031927:hydroquinone