[Carcinogenic effects of sterigmatocystin and deoxynivalenol in NIH mice].
OBJECTIVE
To further explore the carcinogenic activity of Sterigmatocystin (ST) and the possible synergistic carcinogenic effect of deoxynivalenol (DON) in NIH mice.


METHODS
NIH mice were randomly divided into 6 groups, 30 in each. Five groups of mice were given by gastric intubation ST 3 microg/kg, ST 30 microg/kg, ST 3 microg/kg + DON 1.5 microg/kg, ST 30 microg/kg + DON 1.5 microg/kg and DON 1.5 microg/kg respectively, 3 times a week for 24 weeks. The remaining group of mice was given normal saline accordingly, serving as control. All mice were fed with HPLC-confirmed mycotoxin-free food, analysis. The mice were killed and pathologically examined at 58th and 74th weeks.


RESULTS
No pathological changes were found in the control group of mice. Adenocarcinoma of lung was observed in 25.0%, 41.7%, 62.5%, 69.2% and 37.5% of mice given ST 3 microg/kg, ST 30 microg/kg, ST 3 microg/kg + DON 1.5 microg/kg, ST 30 microg/kg + DON 1.5 microg/kg and DON 1.5 microg/kg, respectively. In addition, dysplasia of glandular stomach was detected in 50.0%, 58.3%, 37.5%, 53.8% and 25.0% of mice similarly treated.


CONCLUSIONS
Oral administration of ST or DON can induce adenocarcinoma in lung and dysplasia of glandular stomach in NIH mice. There is synergistic carcinogenic effect when both ST and DON are given.
Huang
Xiang-hua
XH
Laboratory of Experimental Pathology, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
Zhang
Xiang-hong
XH
Li
Yue-hong
YH
Wang
Jun-ling
JL
Yan
Xia
X
Xing
Ling-xiao
LX
Wang
Feng-rong
FR
D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology; D000818:Animals; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000473:pathology; D008297:Male; D051379:Mice; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology; D013241:Sterigmatocystin / Q000633:toxicity*; D014255:Trichothecenes / Q000633:toxicity*
D014255:Trichothecenes; D013241:Sterigmatocystin; C007262:deoxynivalenol