Mast cell distribution in normal adult skin.
OBJECTIVE
To investigate mast cell distribution in normal adult skin to provide a reference range for comparison with mastocytosis.


METHODS
Mast cells (MCs) were counted in uninvolved skin adjacent to basal cell carcinomas and other dermatological disorders in adults.


RESULTS
There was an uneven distribution of MCs in different body sites using the anti-tryptase monoclonal antibody technique. Numbers of MCs on the trunk, upper arm, and upper leg were similar, but were significantly different from those found on the lower leg and forearm. Two distinct groups were formed--proximal and distal. There were 77.0 MCs/mm2 at proximal body sites and 108.2 MCs/mm2 at distal sites. Adjusted for the adjacent diagnosis and age, this difference was consistent. The numbers of MCs in uninvolved skin adjacent to basal cell carcinomas and other dermatological disorders were not different from those in the control group. Differences in the numbers of MCs between the distal and the proximal body sites must be considered when MCs are counted for a reliable diagnosis of mastocytosis. A pilot study in patients with mastocytosis underlined the variation in the numbers of MCs in mastocytosis and normal skin, but showed a considerable overlap. The observed numbers of MCs in adults cannot be extrapolated to children.


CONCLUSIONS
MC numbers varied significantly between proximal and distal body sites and these differences must be considered when MCs are counted for a reliable diagnosis of mastocytosis. There was a considerable overlap between the numbers of MCs in mastocytosis and normal skin.
Janssens
A S
AS
Department of Dermatology and Venereology, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands.
Heide
R
R
den Hollander
J C
JC
Mulder
P G M
PG
Tank
B
B
Oranje
A P
AP
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001132:Arm / Q000033:anatomy & histology; D002196:Capillaries / Q000033:anatomy & histology; D002452:Cell Count; D006801:Humans; D007866:Leg / Q000033:anatomy & histology; D008407:Mast Cells / Q000166:cytology*; D034701:Mastocytosis, Cutaneous / Q000473:pathology; D008875:Middle Aged; D010865:Pilot Projects; D012016:Reference Values; D012867:Skin / Q000098:blood supply / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods
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