[Determination of gallic acid in Melastoma dodecandrum by RP-HPLC].
OBJECTIVE
To establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb.


METHODS
The RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm.


RESULTS
The content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations.


CONCLUSIONS
The method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum.
He
Xun
X
School of Pharmacy, Guiyang Medical College, Guiyang 550004, China. hexun224@gmc.edu.cn
Li
Yong-jun
YJ
Liu
Li-na
LN
Lan
Yan-yu
YY
Wang
Ai-min
AM
Wang
Yong-lin
YL
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D005707:Gallic Acid / Q000032:analysis*; D035322:Melastomataceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D005707:Gallic Acid