Quantification of Enterococcus faecalis and Enterococcus faecium in different foods using rRNA-targeted oligonucleotide probes.
The abundance of Enterococcus faecalis and Enterococcus faecium in different Spanish foods was evaluated by using taxon-specific oligonucleotide probes targeted against extracted rRNA. Two satisfactory methods were developed for RNA extraction. Although the yield and purity of total RNA obtained largely depended on the type of food, method 1 should be recommended. The quantitative results obtained with the oligonucleotide probes DB6 for E. faecium and DB8 for E. faecalis showed that these two species accounted for less than 0.5% of the active microflora in all the food samples tested. These results suggest that enterococci form only a minor portion of the microflora of these products.
Omar
Nabil Ben
NB
Area de Microbiología, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071 Jaén, Spain.
Castro
Araceli
A
Abriouel
Hikmate
H
Lucas
Rosario
R
Pérez
Rubén
R
Martínez-Cañamero
Magdalena
M
Gálvez
Antonio
A
D000818:Animals; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development*; D016984:Enterococcus faecium / Q000235:genetics / Q000254:growth & development*; D005516:Food Microbiology; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000517:prevention & control; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D012329:RNA, Bacterial; D012335:RNA, Ribosomal / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D013030:Spain
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal