title: Metabolites of conjugated isomers of alpha-linolenic acid (CLnA) in the rat.
abstract: Rumelenic (cis-9,trans-11,cis-15 18:3) acid is a naturally occurring conjugated isomer of alpha-linolenic acid (CLnA) in milk fat. Metabolism in rats was studied using a synthetic CLnA mixture, composed mainly by equimolar quantities of cis-9,trans-11,cis-15 and cis-9,trans-13,cis-15 CLnA isomers. Their metabolisms were studied by feeding high quantities of CLnA (150 mg/day) for 4 days to rats that had been reared on a fatfree diet for 2 weeks. After this period, animals were sacrificed and liver and epididymal adipose tissue lipids extracted. Six metabolites of the cis-9,trans-11,cis-15 18:3 CLnA isomers were identified as being cis-7,trans-9,cis-13 16:3, cis-11,trans-13,cis-17 20:3, cis-8,cis-11,trans-13,cis-17 20:4, cis-5,cis-8,cis-11,trans-13,cis-17 20:5, cis-7,cis-10,cis-13,trans-15,cis-19 22:5, and cis-4,cis-7,cis-10,cis-13,trans-15,cis-19 22:6 acids. Two metabolites of cis-9,trans-13,cis-15 18:3 CLnA isomer were also identified by GC-MS as being cis-7,trans-11,cis-13 16:3 and cis-5,cis-8,cis-11,trans-15,cis-17 20:5.
authors.affiliation: Department of Food Science and Nutrition and Dairy Research Center (STELA), Université Laval, Sainte Foy, Québec, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Destaillats
authors.forename: Olivier
authors.initials: O
authors.lastname: Berdeaux
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Sébédio
authors.forename: Pierre
authors.initials: P
authors.lastname: Juaneda
authors.forename: Stéphane
authors.initials: S
authors.lastname: Grégoire
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Chardigny
authors.forename: Lionel
authors.initials: L
authors.lastname: Bretillon
authors.forename: Paul
authors.initials: P
authors.lastname: Angers
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D017962:alpha-Linolenic Acid / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D017962:alpha-Linolenic Acid