A molecular delivery system by using AFM and nanoneedle.
We developed a new low invasive cell manipulation and gene or molecule transfer system in a single living cell by using an atomic force microscope (AFM) and ultra thin needle, a nanoneedle. DNA was immobilized on the surface of the nanoneedle by covalent bonding and avidin-biotin affinity binding. Immobilization of DNA on the nanoneedle was confirmed by measuring the unbinding force between avidin and biotin. The DNA-immobilized nanoneedle was successfully inserted into HEK293 cells. Though TO-PRO-3 iodide staining experiments using confocal microscopy, we observed the immobilized DNA on the surface of the nanoneedle, which was retained after 10 times insertions to and evacuations from a living cell.
Han
Sung Woong
SW
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.
Nakamura
Chikashi
C
Obataya
Ikuo
I
Nakamura
Noriyuki
N
Miyake
Jun
J
D002460:Cell Line; D004247:DNA / Q000008:administration & dosage*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007668:Kidney / Q000502:physiology / Q000648:ultrastructure*; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D009339:Needles; D014162:Transfection / Q000295:instrumentation* / Q000379:methods
D004247:DNA