[Relationship between the expression of insulin-like growth factor I receptors and apoptosis after preliminary chemotherapy, in child and adolescent osteosarcoma].
BACKGROUND
The influence of growth factors and apoptosis on the clinical course of neoplasms is of significant interest for oncologists, especially neoplasms with dynamic changes in proliferation, such as child and adolescent osteosarcoma.


OBJECTIVE
To investigate reciprocal relations between the expression of insulin-like growth factor I receptors (IGF-IR) and index apoptosis ("pre-G l /G 0" fraction, DNA histograms) in osteosarcomas before operation and after preliminary chemotherapy.


METHODS
Flow cytometric analysis was carried out in 20 children and adolescents with osteosarcoma (aged 6 to 18 years) after preliminary chemotherapy. The material for investigation was taken during surgery.


RESULTS
It was shown that higher expression of IGF-IR is associated with lower apoptosis index (% of apoptotic cells) and reversely (significant difference p<O, 05 Student's test).


CONCLUSIONS
Investigations of the above relations are significant for better understanding of the influence of IGF-IR on the disease course and on the preliminary evaluation of cytostatic activity by way of apoptosis initiation.
Rytwiński
Krzysztof
K
Pracownia Cytometrii, Zaklad Immunologii Klinicznej, Instytut Matki i Dziecka, Kasprzaka 17a, 01-211 Warszawa, Poland.
Wnuk
Aleksander
A
Woźniak
Wojciech
W
Rychłowska
Magdalena
M
D000293:Adolescent; D000911:Antibodies, Monoclonal; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology* / Q000601:surgery; D002455:Cell Division / Q000187:drug effects; D002648:Child; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology* / Q000601:surgery; D017526:Receptor, IGF Type 1 / Q000032:analysis* / Q000187:drug effects; D013997:Time Factors
D000911:Antibodies, Monoclonal; D017526:Receptor, IGF Type 1