title: The bacteriophage lambda DNA replication protein P inhibits the oriC DNA- and ATP-binding functions of the DNA replication initiator protein DnaA of Escherichia coli.
abstract: Under the condition of expression of lambda P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the lambda P protein inhibits the binding of both oriC DNA and ATP to the wild-type DnaA protein but not to the rpl DnaA protein. We conclude that the lambda P protein inhibits the binding of oriC DNA and ATP to the wild-type DnaA protein, which causes the inhibition of host DNA synthesis initiation that ultimately leads to bacterial death. A possible beneficial effect of this interaction of lambda P protein with E. coli DNA initiator protein DnaA for phage DNA replication has been proposed.
authors.affiliation: Department of Biochemistry, Bose Institute, Acharya J. C. Bose Birth Centenary Building, P-1/12, CIT Scheme VII M, Kolkata 700 054, India.
authors.forename: Indrani
authors.initials: I
authors.lastname: Datta
authors.forename: Subrata
authors.initials: S
authors.lastname: Sau
authors.forename: Alok Kumar
authors.initials: AK
authors.lastname: Sil
authors.forename: Nitai C
authors.initials: NC
authors.lastname: Mandal
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001426:Bacterial Proteins / Q000502:physiology*; D010582:Bacteriophage lambda / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism / Q000821:virology; D015964:Gene Expression Regulation, Bacterial; D005804:Genes, Lethal; D009154:Mutation / Q000235:genetics; D018741:Replication Origin; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; C024013:DNA replication complex protein, Bacteriophage lambda; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D014764:Viral Proteins; D000255:Adenosine Triphosphate