title: Arginine methylation of MRE11 by PRMT1 is required for DNA damage checkpoint control.
abstract: The role of protein arginine methylation in the DNA damage checkpoint response and DNA repair is largely unknown. Herein we show that the MRE11 checkpoint protein is arginine methylated by PRMT1. Mutation of the arginines within MRE11 severely impaired the exonuclease activity of MRE11 but did not influence its ability to form complexes with RAD50 and NBS1. Cells containing hypomethylated MRE11 displayed intra-S-phase DNA damage checkpoint defects that were significantly rescued with the MRE11-RAD50-NBS1 complex. Our results suggest that arginine methylation regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response.
authors.affiliation: Terry Fox Molecular Oncology Group, Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research and Departments of Oncology and Medicine, McGill University, Montréal, Québec H3T 1E2, Canada.
authors.forename: François-Michel
authors.initials: FM
authors.lastname: Boisvert
authors.forename: Ugo
authors.initials: U
authors.lastname: Déry
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Masson
authors.forename: Stéphane
authors.initials: S
authors.lastname: Richard
mesh_terms: D017766:Acid Anhydride Hydrolases; D001120:Arginine / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D004249:DNA Damage / Q000235:genetics* / Q000502:physiology; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005982:Glutathione Transferase; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D000076228:MRE11 Homologue Protein; D013058:Mass Spectrometry; D008745:Methylation; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D009841:Oligonucleotides; D011484:Protein-Arginine N-Methyltransferases / Q000378:metabolism*; D016196:S Phase / Q000235:genetics / Q000502:physiology; D014161:Transduction, Genetic
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C121766:MRE11 protein, human; C112156:NBN protein, human; D009687:Nuclear Proteins; D009841:Oligonucleotides; D001120:Arginine; D011484:Protein-Arginine N-Methyltransferases; D005982:Glutathione Transferase; D000076228:MRE11 Homologue Protein; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; D045643:DNA Repair Enzymes
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