Development of a novel glucose enzyme fuel cell system employing protein engineered PQQ glucose dehydrogenase.
Glucose dehydrogenase harboring pyrroloquinoline quinone as the prosthetic group (PQQGDH) from Acinetobacter calcoaceticus is an ideal enzyme for the anode of biofuel cell, because of its oxygen insensitivity and high catalytic efficiency. However, the application of PQQGDH for the bioanode is inherently limited because of its instability. Using Ser415Cys mutant whose stability was greatly improved, we constructed the biofuel cell system employing the engineered PQQGDH as the bioanode enzyme and bilirubin oxidase (BOD) as the biocathode, and compared the stability of the biofuel cell with that employing wild-type PQQGDH. The maximum power density was 17.6 microW/cm2 at an external optimal load of 200 k omega. Using Ser415Cys mutant, the lifetime of the biofuel cell system was greatly extended to 152 h, more than six times as that of the biofuel cell employing the wild-type.
Yuhashi
Noriko
N
Department of Life Science and Biotechnology, Tokyo University of Agriculture and Technology, Nakamachi 2-24-16, Koganei, Tokyo 184-8588, Japan.
Tomiyama
Masamitsu
M
Okuda
Junko
J
Igarashi
Satoshi
S
Ikebukuro
Kazunori
K
Sode
Koji
K
D016954:Acinetobacter calcoaceticus / Q000201:enzymology* / Q000235:genetics*; D019943:Amino Acid Substitution; D011211:Electric Power Supplies; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004735:Energy Transfer; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005947:Glucose / Q000737:chemistry*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010865:Pilot Projects; D015202:Protein Engineering / Q000379:methods*
D042843:Glucose 1-Dehydrogenase; D005947:Glucose