Disruption of lateral olivocochlear neurons via a dopaminergic neurotoxin depresses sound-evoked auditory nerve activity.
We applied the dopaminergic (DA) neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to the guinea pig cochlear perilymph. Immunolabeling of lateral olivocochlear (LOC) neurons using antibodies against synaptophysin was reduced after the MPTP treatment. In contrast, labeling of the medial olivocochlear innervation remained intact. As after brainstem lesions of the lateral superior olive (LSO), the site of origin of the LOC neurons, the main effect of disrupting LOC innervation of the cochlea via MPTP was a depression of the amplitude of the compound action potential (CAP). CAP amplitude depression was similar to that produced by LSO lesions. Latency of the N1 component of the CAP, and distortion product otoacoustic emission amplitude and adaptation were unchanged by the MPTP treatment. This technique for selectively lesioning descending LOC efferents provides a new opportunity for examining LOC modulation of afferent activity and behavioral measures of perception.
Le Prell
Colleen G
CG
Kresge Hearing Research Institute, University of Michigan Medical School, Ann Arbor, MI 48109-0506, USA. colleeng@umich.edu
Halsey
Kärin
K
Hughes
Larry F
LF
Dolan
David F
DF
Bledsoe
Sanford C
SC
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology; D000200:Action Potentials; D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology; D000818:Animals; D003056:Cochlear Nerve / Q000502:physiology*; D017626:Cochlear Nucleus / Q000473:pathology / Q000502:physiology*; D003714:Denervation; D004298:Dopamine / Q000502:physiology*; D015259:Dopamine Agents / Q000494:pharmacology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009498:Neurotoxins / Q000494:pharmacology; D009847:Olivary Nucleus / Q000473:pathology / Q000502:physiology*; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects / Q000502:physiology
D015259:Dopamine Agents; D009498:Neurotoxins; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D004298:Dopamine
Neurochem Res. 1998 Aug;23(8):1133-8
9704604
Neuroscience. 1999 Apr;90(1):131-8
10188940
J Neurophysiol. 1995 Aug;74(2):598-615
7472367
Brain Res. 1987 Sep 1;419(1-2):347-51
2890408
Prog Neurobiol. 1997 Oct;53(3):331-80
9364616
J Neurochem. 1993 Oct;61(4):1191-206
8376979
Neuroreport. 2001 Mar 26;12(4):803-7
11277587
Acta Otolaryngol. 1993 May;113(3):330-4
8100108
J Pharmacol Exp Ther. 1969 Feb;165(2):300-9
5763065
Hear Res. 1996 May;95(1-2):161-7
8793517
Hear Res. 2005 Mar;201(1-2):99-108
15721565
J Neurosci. 2000 Jul 1;20(13):5163-9
10864973
J Neurocytol. 1999 Mar;28(3):197-206
10617902
Hear Res. 2000 Oct;148(1-2):124-36
10978830
J Comp Neurol. 1996 Aug 05;371(4):621-32
8841914
Hear Res. 1990 Nov;49(1-3):209-23
1705540
J Assoc Res Otolaryngol. 2000 Aug;1(1):82-8
11548239
Hear Res. 1995 Oct;90(1-2):202-11
8974998
J Comp Neurol. 1999 Mar 29;406(1):72-86
10100893
Neuroscience. 1993 May;54(1):133-42
8100046
Hear Res. 1998 Oct;124(1-2):17-26
9822899
Prog Neurobiol. 1995 Dec;47(6):449-76
8787031
Physiol Rev. 1993 Apr;73(2):309-73
8097330
J Histochem Cytochem. 1984 Aug;32(8):839-43
6379037
Neuroscience. 2004;125(3):725-33
15099686
J Acoust Soc Am. 1970 Oct;48(4):966-77
5480391
Eur J Neurosci. 2001 Sep;14(6):977-86
11595036
Hear Res. 1985 Mar;17(3):249-58
2862132
J Neurosci. 1998 May 15;18(10):3859-69
9570815
J Neurophysiol. 1991 May;65(5):1158-69
1651373
Neuroreport. 1996 Mar 22;7(4):889-94
8724667
Am J Otolaryngol. 1986 Mar-Apr;7(2):100-6
2870655
Hear Res. 1994 Apr;74(1-2):122-34
8040083
Hear Res. 1999 Feb;128(1-2):75-9
10082286
Life Sci. 2000 Jun 8;67(3):241-50
10983868
Brain Res. 1991 Mar 22;544(1):94-100
1855140
Brain Res. 1984 May 28;301(1):187-91
6610458
J Neurophysiol. 1956 Sep;19(5):424-37
13367873
J Comp Neurol. 1987 Jun 22;260(4):605-18
3611413
Acta Otolaryngol. 1993 Jul;113(4):512-8
8379307
J Neurophysiol. 1988 Aug;60(2):549-68
3171641
Toxicol Lett. 2003 Apr 4;139(2-3):135-51
12628749
Neurotoxicol Teratol. 1999 Mar-Apr;21(2):169-75
10192277
Ann N Y Acad Sci. 1999 Nov 28;884:305-11
10842602
Hear Res. 1987;30(1):33-8
2890616
J Histochem Cytochem. 1988 Jul;36(7):797-801
2898496
Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1657-60
11818566
Hear Res. 1989 Oct;42(1):113-7
2584156
Hear Res. 1987;30(2-3):135-46
2890617
Hear Res. 2000 Jun;144(1-2):89-96
10831868
Hear Res. 2002 Dec;174(1-2):124-32
12433403
Neurosci Lett. 1999 Jul 2;269(1):49-51
10821642
J Neurophysiol. 2003 Nov;90(5):3178-200
14615429
Ear Hear. 1994 Dec;15(6):422-31
7895938
Eur J Neurosci. 1997 Feb;9(2):356-67
9058055
Physiol Rev. 2001 Jul;81(3):1305-52
11427697
J Assoc Res Otolaryngol. 2002 Dec;3(4):457-78
12486600
Hear Res. 2004 Jan;187(1-2):122-30
14698093
J Chromatogr. 1987 Jul 10;398:279-87
3498735
Hear Res. 1992 Dec;64(1):1-5
1362722
Hear Res. 1992 Aug;61(1-2):106-16
1326504
Hear Res. 2003 Nov;185(1-2):65-76
14599694
Noise Health. 2003 Jul-Sep;5(20):1-17
14558888
J Acoust Soc Am. 1992 Nov;92(5):2662-82
1479129
J Acoust Soc Am. 2004 Aug;116(2):1044-56
15376671
Hear Res. 1988 May;33(2):97-113
3397330
J Assoc Res Otolaryngol. 2001 Sep;2(3):268-78
11669399
Behav Brain Res. 2001 Sep 28;124(1):9-18
11423161
J Neurophysiol. 2003 Nov;90(5):2941-9
12904337
Hear Res. 1999 Dec;138(1-2):65-72
10575115
Toxicol Lett. 1989 Aug;48(2):121-49
2672418
Nature. 1961 Dec 30;192:1263-5
13885734
Brain Res Mol Brain Res. 2000 Mar 10;76(1):25-35
10719212
Hear Res. 1993 Jun;68(1):97-106
8376219
Hear Res. 1997 May;107(1-2):46-52
9165346
Acta Otolaryngol. 1995 Mar;115(2):222-6
7610809
Hear Res. 1991 Sep;55(1):133-42
1684359
Hear Res. 1997 Jun;108(1-2):89-111
9213126
Psychopharmacol Bull. 1984 Summer;20(3):548-53
6332333
Hear Res. 2003 Nov;185(1-2):97-108
14599697
J Neurol Sci. 1988 Aug;86(1):97-110
3262717
J Neurosci. 1996 Jan;16(1):325-32
8613799
ORL J Otorhinolaryngol Relat Spec. 1993;55(1):3-6
8382781
Hear Res. 1987;25(1):69-76
3804858
Ann N Y Acad Sci. 1999 Nov 28;884:292-304
10842601
Hear Res. 1998 Nov;125(1-2):147-53
9833968
Int J Dev Neurosci. 1997 Jul;15(4-5):631-43
9263039
Br J Audiol. 1994 Aug-Oct;28(4-5):185-91
7735146
Mov Disord. 1998;13 Suppl 1:35-8
9613716
J Acoust Soc Am. 1996 Jun;99(6):3572-84
8655789
Acta Otolaryngol Suppl. 1988;447:36-45
2903609
Acta Otolaryngol. 1995 Jan;115(1):27-30
7762379
Anat Embryol (Berl). 1992;185(1):1-16
1736680
Hear Res. 2004 Apr;190(1-2):75-86
15051131
J Assoc Res Otolaryngol. 2003 Jun;4(2):276-90
12943378
Hear Res. 2001 May;155(1-2):143-51
11335084
Acta Otolaryngol. 1974 Jan-Feb;77(1):56-65
4829054
Exp Brain Res. 2003 Dec;153(4):499-513
14557913
J Neurosci. 1995 May;15(5 Pt 1):3667-78
7751937
Hear Res. 1987;29(2-3):179-94
3624082
Neuroreport. 1997 Feb 10;8(3):591-6
9106729
Neuroscience. 1983 Jul;9(3):621-30
6353274
J Physiol. 1975 May;247(2):407-28
1097639
J Neurosci. 2000 Jun 15;20(12):4701-7
10844039
Hear Res. 1999 Aug;134(1-2):57-70
10452376
Neuroscience. 2001;106(3):589-601
11591459
Eur Arch Otorhinolaryngol. 1995;252(5):270-4
7576583
Am J Otol. 1996 May;17(3):486-92
8817030
Eur J Neurosci. 1992;4(10):981-992
12106433
Behav Brain Res. 2001 Nov 1;125(1-2):109-25
11682102
Hear Res. 1998 Dec;126(1-2):67-74
9872135
Hear Res. 1997 Jun;108(1-2):74-82
9213124