Arteriolar responses to vasodilator stimuli and elevated P(O2) in renin congenic and Dahl salt-sensitive rats.
OBJECTIVE
Angiotensin II suppression leads to impaired vascular relaxation in normotensive animals on a high-salt diet. The goal of this study was to determine whether normal vascular reactivity could be restored by transferring the chromosomal region carrying the Dahl salt-resistant (R) renin gene into the Dahl salt-sensitive (SS) genetic background in a strain of renin congenic rats (RGRR).


METHODS
Male RGRR and SS rats were fed low-salt (0.4%) and high-salt (4%) diets for 4 weeks. The responses of cremaster muscle arterioles to acetylcholine (ACh), sodium nitroprusside (SNP), and elevated PO2 were assessed using video microscopy.


RESULTS
ACh-induced dilation was significantly enhanced in RGRR on a high-salt diet compared to SS rats, while dilation to the NO donor SNP was similar in both strains. A high-salt diet significantly enhanced arteriolar constriction in response to elevated PO2, in both SS and RGRR rats.


CONCLUSIONS
These data suggest that transfer of the chromosomal region containing the renin gene is crucial in the recovery of ACh-induced dilation of arterioles in RGRR rats vs. SS rats, and that factors in the SS genetic background contribute to an enhanced sensitivity to elevated PO2, independent of genes on chromosome 13.
Drenjancevic-Peric
Ines
I
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
Greene
Andrew S
AS
Kunert
Mary Pat
MP
Lombard
Julian H
JH
D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000502:physiology*; D033481:Chromosomes, Mammalian; D008297:Male; D018715:Microscopy, Video; D009132:Muscles / Q000098:blood supply; D010100:Oxygen / Q000097:blood / Q000494:pharmacology*; D051381:Rats; D020303:Rats, Inbred Dahl; D012083:Renin / Q000235:genetics* / Q000502:physiology; D012084:Renin-Angiotensin System; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D019076:Transgenes; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
D014665:Vasodilator Agents; D012965:Sodium Chloride; D012083:Renin; D010100:Oxygen