A rapid method to quantify pro-oxidant activity in cultures of wood-decaying white-rot fungi.
A new, rapid method for evaluation of lipid peroxidation promoting (pro-oxidant) activity in cultures of wood-decaying fungi was developed. The method is based on measurement of the rate of oxygen consumption in the reaction of linoleic acid peroxidation initiated by fungal culture filtrates. The liquid cultures of the white-rot fungi Bjerkandera adusta and Phanerochaete chrysosporium grown on wheat straw-containing glucose-peptone-corn steep liquor medium possessed significant levels of the pro-oxidant activity. Other white-rot fungi producing manganese peroxidase (MnP) were also found to show the activity. MnP demonstrated a crucial role as the major pro-oxidant agent in the fungal cultures. The total pro-oxidant activity may be considered as net result of the peroxidation by MnP and the inhibition by antioxidant compounds present in the fungal culture fluids.
Kapich
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AN
Department of Applied Chemistry and Microbiology, University of Helsinki, Viikinkaari 9, Viikki Biocenter 1, P.O.Box 56, 00014 Helsinki, Finland. alexander.kapich@helsinki.fi
Prior
B A
BA
Lundell
T
T
Hatakka
A
A
D002021:Buffers; D007525:Isoelectric Focusing; D007700:Kinetics; D019787:Linoleic Acid / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D002021:Buffers; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D019787:Linoleic Acid; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase