Perfluoroarylazide derivatives of 2'-O-modified oligoribonucleotides: efficient reagents for RNA photomodification.
Site-specific photomodification of the 5'-terminal fragment of MDR1 mRNA by perfluoroarylazide derivatives of 2'-O-modified (2'-O-methyl or 2'-O-tetrahydropyranyl) oligoribo- and oligodeoxyribonucleotides was investigated. The conjugates built of 2'-O-modified oligoribonucleotides demonstrate beneficial features compared with their deoxyribo analogs: the extent of RNA modification by 2'-O-modified oligoribonucleotides and oligodeoxyribonucleotide conjugates was 40- 50% and 20%, respectively.
Novopashina
D S
DS
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
Kulikov
R N
RN
Kuznetsova
M A
MA
Venyaminova
A G
AG
Zenkova
M A
MA
Vlassov
V V
VV
D001386:Azides / Q000737:chemistry*; D020029:Base Pairing; D019450:Genes, MDR / Q000235:genetics*; D009843:Oligoribonucleotides / Q000737:chemistry* / Q000235:genetics; D010777:Photochemistry; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
D001386:Azides; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012260:Ribonucleases