Estrogen receptor-beta, but not estrogen receptor-alpha, is expressed in prolactin neurons of the female rat paraventricular and supraoptic nuclei: comparison with other neuropeptides.
Estrogen receptor-alpha (ER-alpha) and ER-beta exhibit fine differences in their distributions in the rodent forebrain, and one such difference is observed in the paraventricular (PVN) and supraoptic (SON) nuclei. To investigate the functional significance of ER in these brain areas, we examined the neuropeptide characteristics of ER-expressing neurons in the PVN and SON of female rats by using dual-label immunocytochemistry. The distributions of ER-alpha immunoreactivity (ir) and ER-beta ir were nonoverlapping in the PVN and SON. Nuclear ER-alpha ir was found in a population of thyrotropin-releasing hormone (TRH)-expressing neurons in the PVN (5.93% +/- 1.20% SEM), but not in any other identified cell phenotype of the PVN and SON. The phenotype of neurons with the highest percentage expressing ER-beta was found to be prolactin (PRL) immunoreactive in both the parvocellular (84.95% +/- 4.11%) and the magnocellular (84.76% +/- 3.40%) parts of the PVN as well as the SON (87.57% +/- 4.64%). Similarly, most vasopressin-immunoreactive neurons were also ER-beta positive in the PVN (66.14% +/- 2.47%) and SON (72.42% +/- 4.51%). In contrast, although a high percentage of oxytocin (OXY) neurons coexpressed ER-beta in the PVN (84.39% +/- 2.99%), there was very little ER-beta/OXY colocalization in the SON. Low levels of corticotropin-releasing hormone neurons also expressed ER-beta ir in the PVN (12.57% +/- 1.99%), but there was no ER-beta colocalization with TRH. In summary, these findings further support the possibility of direct effects of estrogen on neuropeptide expression and implicate estrogen involvement in the regulation of various aspects of neuroendocrine function.
Suzuki
Shotaro
S
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA.
Handa
Robert J
RJ
D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis; D047628:Estrogen Receptor alpha / Q000096:biosynthesis*; D047629:Estrogen Receptor beta / Q000096:biosynthesis*; D005260:Female; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D010121:Oxytocin / Q000096:biosynthesis; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D011388:Prolactin / Q000502:physiology*; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013495:Supraoptic Nucleus / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000096:biosynthesis
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D009479:Neuropeptides; C034477:RNA primers; D001127:Arginine Vasopressin; D010121:Oxytocin; D013973:Thyrotropin-Releasing Hormone; D012313:RNA; D011388:Prolactin; D003346:Corticotropin-Releasing Hormone