Maize genes induced by herbivory and volicitin.
In crop plants, both mechanical damage and insect attack trigger rapid changes in gene transcription. We investigated whether insect herbivory differs from a general wound response, and if so, is the induction specific to the pest/host plant interaction? Herbivory by beet armyworm (BAW; Spodoptera exigua) caterpillars on maize results in a unique pattern of volatile compounds not triggered by wounding alone that attracts the generalist parasitoid Cotesia marginiventris. Caterpillar-induced volatile emission can be mimicked when a component of the BAW oral secretions (N-(17-hydroxylinolenoyl)-L-glutamine) termed volicitin, is applied to wounded leaves. We identified genes that are affected by BAW feeding by comparing volicitin treatment with wounding alone. We compared cDNAs from these two populations by isolating genes from a subtractive library and using reverse northerns. Virtual northern blots confirmed these results and further showed that BAW infestation affected the expression of these genes. In some cases, BAW feeding inhibited the expression of volicitin-induced genes, suggesting the role of additional bioactive components in caterpillar regurgitate. Transcripts involved in volatile production are increased by volicitin and BAW infestation treatments, and are also detectable at low levels in mechanically wounded leaves. Finally, we identified three new sesquiterpene cyclase genes that are induced by volicitin.
Lawrence
Susan D
SD
Insect Biocontrol Laboratory, USDA-ARS, BARC-West, Beltsville, MD, USA. Lawrencs@ba.ars.usda.gov
Novak
Nicole G
NG
D000818:Animals; D001483:Base Sequence; D019755:Carbon-Carbon Lyases / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D017343:Genes, Plant; D005973:Glutamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006790:Host-Parasite Interactions / Q000235:genetics*; D010935:Plant Diseases / Q000469:parasitology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera / Q000502:physiology*; D014158:Transcription, Genetic; D014835:Volatilization / Q000187:drug effects; D003313:Zea mays / Q000235:genetics / Q000469:parasitology*; D017962:alpha-Linolenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*
D018076:DNA, Complementary; C419781:volicitin; D017962:alpha-Linolenic Acid; D005973:Glutamine; D019755:Carbon-Carbon Lyases; C051130:trichodiene synthetase