[Progress on phage antibody library technology].
The exterior protein gene and the specific protein gene coded on the surface of phage can be fusedly expressed on the surface of phage with the recently developed technology-phage display technique. Through the PCR technique, the whole light chain genes and heavy chain genes can be amplified. Cloning these genes into lambda phage expression vectors and constructing phage antibody library through antigen adsorption-elution-proliferation, many targeting clones could be selected and the responding mono-clone antibody could be produced. Detailed progress in this regard was reviewed in the paper.
Zhao
Yumei
Y
Shenzhen Institute of Landscape Garden, Shenzhen 518003, China.
Fang
Shisong
S
Zhao
Shujin
S
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D019151:Peptide Library; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library