[Malignant transformation of a human oral squamous cell carcinoma cell strain OSC-19 by EBV encoded small RNAs].
OBJECTIVE
To observe the growth characteristics of the EBERs-transfected OSC (oral squamous carcinoma)-19 cells, through generated overexpression of EBERs in OSC-19 cells.


METHODS
EBERs fragment were amplified by PCR from EBV-positive NPC-KT cell DNA. Using molecular biologic technique, we constructed the EBERs-expressing plasmids, which contained, 4 or 10 tandem repeats of the EBERs. The OSC-19 cells were transfected with those plasmids, respectively. We demenstrated the overexpression of EBERs in EBERs-transfected OSC-19 cells by Northern Blot analysis, and compared the growth characteristics in culture and colony formation in soft agar of Hygr- and EBERs-transfected OSC-19 cells.


RESULTS
10EBERs-transfected OSC-19 cells lost cell-to-cell contacts and acquired long spindle-shape morphology in culture and formed colonies in soft agar. However, parental and Hygr-transfected OSC-19 cells scarcely did.


CONCLUSIONS
In this study, we established 10 EBERs-transfected OSC-19 cells, which overexpressing the EBERs and showed that it has a scattering activity in culture and a role in anchorage-independent growth in soft agar. These results indicate that EBERs has a role of malignant transformation in OSC-19 cells. However, the 10 EBERs-transfected OSC-19 cell provides a useful epithelial model for studying the function of EBERs.
Ren
Qingchun
Q
Department of Otolaryngology, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Zuo
Tengbo
T
Lü
Hongguang
H
D002294:Carcinoma, Squamous Cell / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics*; D014062:Tongue Neoplasms / Q000821:virology*; D014162:Transfection; D014407:Tumor Cells, Cultured
C099537:Epstein-Barr virus encoded RNA 1; C106732:Epstein-Barr virus encoded RNA 2; D012367:RNA, Viral