title: Cultivation of endothelial cells on adhesive protein-free synthetic polymer gels.
abstract: Various hydrogels without modification by any cell adhesive proteins have been investigated as cell scaffolds. The present study shows that bovine fetal aorta endothelial cells can adhere, spread, proliferate, and reach confluence on poly(acrylic acid), poly(sodium p-styrene sulfonate), and poly(2-acrylamido-2- methyl-1-propanesulfonic sodium) gels, whereas cells reach subconfluence on poly(vinyl alcohol) and poly(methacrylic acid) gels. The proliferation behavior was sensitive to both hydrogel charge density and crosslinker concentration. The relationship between cell proliferation and zeta potential of gels was discussed. It was found that hydrogels with a negative zeta potential higher than about 20 mV facilitates cell proliferation.
authors.affiliation: Graduate School of Science, Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Yong Mei
authors.initials: YM
authors.lastname: Chen
authors.forename: Nozomi
authors.initials: N
authors.lastname: Shiraishi
authors.forename: Hideo
authors.initials: H
authors.lastname: Satokawa
authors.forename: Akira
authors.initials: A
authors.lastname: Kakugo
authors.forename: Tetsuharu
authors.initials: T
authors.lastname: Narita
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Osada
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Joji
authors.initials: J
authors.lastname: Ando
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D011108:Polymers
chemical_list: D011108:Polymers