title: Splicing of rhesus rhadinovirus R15 and ORF74 bicistronic transcripts during lytic infection and analysis of effects on production of vCD200 and vGPCR.
abstract: Rhesus macaque rhadinovirus (RRV) is the rhesus macaque homologue of human herpesvirus 8 (HHV-8). Here we examine expression of RRV R15 and ORF74, homologues of K14 and ORF74 of HHV-8, respectively. As in HHV-8, transcripts encoding RRV R15 and ORF74 are bicistronic. However, unlike what has been suggested for HHV-8, RRV R15- and ORF74-encoding transcripts are expressed late during lytic infection and undergo unique splicing events that result in the production of transcripts capable of encoding vGPCR, as well as membrane-associated and secreted forms of vCD200. The alternative splicing for vCD200 has implications for viral pathogenesis.
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health and Science University, West Campus, 505 N.W. 185th Ave., Beaverton, OR 97006, USA.
authors.forename: Carly L
authors.initials: CL
authors.lastname: Pratt
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Estep
authors.forename: Scott W
authors.initials: SW
authors.lastname: Wong
mesh_terms: D015152:Blotting, Northern; D005814:Genes, Viral; D016366:Open Reading Frames; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012367:RNA, Viral / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018144:Rhadinovirus / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012322:RNA Precursors; D012367:RNA, Viral; D043562:Receptors, G-Protein-Coupled; D014764:Viral Proteins
references.citation: Immunity. 2000 Aug;13(2):233-42
references.pmid: 10981966
references.citation: Science. 2000 Dec 1;290(5497):1768-71
references.pmid: 11099416
references.citation: Virology. 2001 Aug 15;287(1):62-70
references.pmid: 11504542
references.citation: J Virol. 2002 Apr;76(7):3421-39
references.pmid: 11884567
references.citation: J Virol. 2003 Feb;77(3):1738-46
references.pmid: 12525607
references.citation: J Virol. 2004 Jul;78(14):7667-76
references.pmid: 15220441
references.citation: J Exp Med. 1999 Sep 20;190(6):827-40
references.pmid: 10499921
references.citation: J Virol. 1999 Apr;73(4):3040-53
references.pmid: 10074154
references.citation: Virology. 1999 Apr 25;257(1):84-94
references.pmid: 10208923
references.citation: J Virol. 1999 Jul;73(7):6006-14
references.pmid: 10364352
references.citation: J Virol. 1999 Jul;73(7):6177-81
references.pmid: 10364379
references.citation: J Immunol. 1999 Aug 1;163(3):1654-60
references.pmid: 10415071
references.citation: J Virol. 1997 Dec;71(12):9764-9
references.pmid: 9371642