[Studies on the performance and applications of chloropropyl bonded silica. I. Separation of disubstituted benzene isomers and prostaglandin epimers].
The performance of chloropropyl bonded silica (CPS) in HPLC and its applications in separation of disubstituted benzene isomers and prostaglandin epimers were studied. The CPS column has high column efficiency and the kinetic curves illustrate it is suitable for fast analysis. The effect of composition and pH value of mobile phase (methanol/water or methanol/20 mmol/L H3PO4-NaOH buffer) on the retention of the solutes were investigated. Generally, the main retention mechanism of solutes was hydrophobic interaction, however there also exited polar adsorption interaction, ion-dipole interaction etc. When the mobile phase condition changed, the main separating mechanism may be changed with CPS column. So, with increasing pH value of mobile phase, the retention of isomers of nitrophenol exhibited a parabolic change. The chromatographic condition was optimized, and the isomers of nitrophenol, aminophenol and toluidine etc. as well as the epimers of 15-methyl-PGF2alpha were separated very well on the column. Moreover, the speed of separation was faster than that in general reversed-phase system. CPS showed to have good stability to heat and solvent and it was also a very stable intermediate to prepare bonded phase with various functional groups.
Gong
Y
Y
Department of Chemistry, Wuhan University, Wuhan, 430072.
Ye
N
N
Xiao
X
X
Da
S
S
Wang
Z
Z
D000327:Adsorption; D001554:Benzene / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D011453:Prostaglandins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
D011453:Prostaglandins; D012822:Silicon Dioxide; D001554:Benzene