Salicylic acid triggers genotoxic adaptation to methyl mercuric chloride and ethyl methane sulfonate, but not to maleic hydrazide in root meristem cells of Allium cepa L.
Salicylic acid (SA), 0.01 mM, a signalling phytohormone, was tested for induction of adaptive response against genotoxicity of methyl mercuric chloride (MMCl), 0.013 mM; ethylmethane sulfonate (EMS), 2.5 mM, or maleic hydrazide (MH), 5 mM, in root meristem cells of Allium cepa. Induction of adaptive response to EMS by hydrogen peroxide (H2O2), 1 mM, and yet another secondary signal molecule was tested for comparison. Assessed by the incidence of mitoses with spindle and/or chromosome aberration and micronucleus, the findings provided evidence that SA-conditioning triggered adaptive response against the genotoxic-challenges of MMCl and EMS, but failed to do so against MH. H2O2, which is known to induce adaptive response to MMCl and MH, failed to induce the same against EMS in the present study. The findings pointed to the possible role of signal transduction in the SA-induced adaptive response to genotoxic stress that perhaps ruled out an involvement of H2O2.
Patra
Jita
J
Genecology and Tissue Culture Laboratory, Department of Botany, Berhampur University, Berhampur 760007, India.
Sahoo
Malaya K
MK
Panda
Brahma B
BB
D000220:Adaptation, Biological; D000818:Animals; D002869:Chromosome Aberrations; D005020:Ethyl Methanesulfonate / Q000494:pharmacology / Q000633:toxicity*; D006540:Herbicides / Q000494:pharmacology / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008300:Maleic Hydrazide / Q000494:pharmacology / Q000633:toxicity*; D018519:Meristem / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008767:Methylmercury Compounds / Q000494:pharmacology / Q000633:toxicity*; D015162:Micronucleus Tests; D008938:Mitosis; D009153:Mutagens / Q000494:pharmacology / Q000633:toxicity; D019697:Onions / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D008941:Spindle Apparatus / Q000187:drug effects
D006540:Herbicides; D008767:Methylmercury Compounds; D009153:Mutagens; D016877:Oxidants; D010937:Plant Growth Regulators; D005020:Ethyl Methanesulfonate; D006861:Hydrogen Peroxide; D020156:Salicylic Acid; C004925:methylmercuric chloride; D008300:Maleic Hydrazide