Identification of anthocyanins in Rhamnus alaternus L. berries.
Anthocyanin composition in berries of Rhamnus alaternus L., a perennial wild shrub typical of the Mediterranean area, was determined for the first time. The pigments were extracted from the berries with 0.1% HCl in methanol and purified using a C-18 solid-phase cartridge. High-performance liquid chromatography diode array detection-mass spectrometry analysis showed that delphinidin 3-O-rutinoside represented about 62.4% of the total pigments. Other anthocyanins were 3-O-rutinoside derivatives of cyanidin (8.4%), petunidin (15.8%), pelargonidin (4.7%), and peonidin and malvidin (8.7%). The concomitant presence of the six most common anthocyanidins suggested that R. alaternus berries, besides being a good pigment source, could also be a useful tool for anthocyanin identification.
Longo
Luigia
L
Dipartimento di Ingegneria dell'Innovazione, Università di Lecce, via per Arnesano km 1, 73100 Lecce, Italy.
Vasapollo
Giuseppe
G
Rescio
Leonardo
L
D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry; D010860:Pigments, Biological / Q000032:analysis; D031955:Rhamnus / Q000737:chemistry*
D000872:Anthocyanins; D010860:Pigments, Biological; C017185:delphinidin