[Relationship between CD8+ T-cell CD28 expression and TCM differentiation type in patients with chronic aplastic anemia].
OBJECTIVE
To explore the relationship between CD8+ T-cell CD28 molecular expression in peripheral blood and TCM type in patients with chronic aplastic anemia (CAA).


METHODS
Using flow cytometry to detect the CD28 expression in 45 in-patients or out-patients and 24 healthy subjects for control. And the relation with TCM type was analyzed from the immunological aspect.


RESULTS
(1) The levels of CD8, CD28, CD8+ CD28+ expression and CD8+ CD28+/CD8+ CD28- were all higher in the CAA patients than those in the healthy subjects (P < 0.05 or P < 0.01). (2) The levels of CD28, CD8+ CD28+ expression and CD8+ CD28+/CD8+ CD28- were all higher in the CAA patients of Shen-Yin deficiency type than those in the CAA patients of Shen-Yang deficiency type (P < 0.05 or P < 0.01).


CONCLUSIONS
(1) The abnormal high expression of peripheral blood co-stimulatory molecules CD28 suggested CD28 disorder may play an important role in immuno-pathogenesis of CAA. (2) The levels of peripheral CD28, CD8+ CD28+ expression and CD8+ CD28+/CD8+ CD28- can be taken as an objective indexes for TCM typing of CAA, which was disordered more severe in patients of Shen-Yin deficiency type than in those of Shen-Yang deficiency type.
Deng
Ting
T
Department of Hematology, Shanghai Municipal Hospital of TCM, Shanghai 200071. grace.dun@hotmail.com
Wang
Yun-lu
YL
Hu
Qi
Q
Sun
Si-ming
SM
Zhang
Xiao-ying
XY
D000328:Adult; D000741:Anemia, Aplastic / Q000145:classification / Q000175:diagnosis* / Q000276:immunology; D018106:CD28 Antigens / Q000096:biosynthesis*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002908:Chronic Disease; D003937:Diagnosis, Differential; D006801:Humans; D007674:Kidney Diseases / Q000276:immunology; D008297:Male; D008516:Medicine, Chinese Traditional; D016711:Yang Deficiency / Q000276:immunology; D016710:Yin Deficiency / Q000276:immunology
D018106:CD28 Antigens