[Gene expression differences between the anti-BPDE-transformed and normal cells by suppression subtractive hybridization].
OBJECTIVE
To screen the gene expression differences between the BPDE-transformed and normal cells.


METHODS
Suppression subtractive hybridization was performed to profile differentially expressed genes between BPDE and normal 16HBE cells. The BPDE-transformed cells was used as tester, and the normal cells as driver. The nested PCR products were inserted into TA vector after two subtractive hybridization and two PCR. The clones were picked up randomly and analyzed with PCR. Dot blot was used to test the same source with the tester. The overexpressed cDNA fragments in BPDE cells were sequenced and compared with known genes in Genbank.


RESULTS
Seven genes were identified being overexpressed in BPDE-transformed cells: translation elongation factor 1 alpha 1, mitochondrion gene, solute carrier family 25, EphA2, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, lactate dehydrogenase A (LDHA), tripartite motif-containing 28 (TRIM28), etc. One fragment could not be found in the known genes, it could be found in the EST database.


CONCLUSIONS
These over-expressed genes may be related to the carcinogenesis of BPDE, in addition, one unknown gene may be responsible for the malignant transforming effect of BPDE.
An
Shejuan
S
Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China.
Chen
Jiakun
J
Chen
Xuemin
X
Zhao
Yanfeng
Y
Liu
Lili
L
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D002273:Carcinogens; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide