Circadian-associated rice pseudo response regulators (OsPRRs): insight into the control of flowering time.
A small family of plant proteins, designated PSEUDO RESPONSE REGULATORS (PRRs), is crucial for a better understanding of the molecular link between circadian rhythm and photoperiodic control of flowering time in the dicotyledonous model plant Arabidopsis thaliana. Recently, we showed that the monocotyledonous model plant Oryza sativa also has homologous members of the OsPRR family (Oryza sativa PRR). In the previous experiments with rice, we mainly characterized a japonica variety (Nipponbare). By employing an indica variety (Kasalath), in this study we further characterized OsPRRs with reference to the photoperiod sensitivity Hd (Heading date) QTL (quantitative trait loci) implicated in the control of flowering time in rice. The circadian-controlled and sequential expression profiles of the five OsPRR genes were observed not only for Nipponbare but also for Kasalath. Then each of these OsPRR genes was mapped on the rice chromosomes. Among these OsPRR genes, OsPRR37 was mapped very closely to Hd2-QTL, which was identified as the major locus that enhances the photoperiod sensitivity of flowering in Nipponbare. Furthermore, we found that Kasalath has a severe mutational lesion in the OsPRR37 coding sequence.
Murakami
Masaya
M
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
Matsushika
Akinori
A
Ashikari
Motoyuki
M
Yamashino
Takafumi
T
Mizuno
Takeshi
T
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D002940:Circadian Rhythm / Q000502:physiology*; D035264:Flowers / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D017440:Photoperiod; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D013997:Time Factors
D010940:Plant Proteins