Molecular cloning, identification and characteristics of a novel isoform of carbamyl phosphate synthetase I in human testis.
A gene coding a novel isoform of carbamyl phosphate synthetase I (CPS1) was cloned from a human testicular library. As shown by cDNA microarray hybridization, this gene was expressed at a higher level in human adult testes than in fetal testes. The full length of its cDNA was 3831 bp, with a 3149 bp open reading frame, encoding a 1050-amino-acid protein. The cDNA sequence was deposited in the GenBank (AY317138). Sequence analysis showed that it was homologous to the human CPS1 gene. The putative protein contained functional domains composing the intact large subunit of carbamoyl phosphate synthetase, thus indicated it has the capability of arginine biosynthesis. A multiple tissue expression profile showed high expression of this gene in human testis, suggesting the novel alternative splicing form of CPS1 may be correlated with human spermatogenesis.
Huo
Ran
R
Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, China.
Zhu
Hui
H
Lu
Li
L
Ying
Lanlan
L
Xu
Min
M
Xu
Zhiyang
Z
Li
Jianmin
J
Zhou
Zuomin
Z
Sha
Jiahao
J
D000328:Adult; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001120:Arginine / Q000096:biosynthesis; D001483:Base Sequence; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005333:Fetus / Q000201:enzymology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013091:Spermatogenesis; D013737:Testis / Q000196:embryology / Q000201:enzymology*; D014018:Tissue Distribution
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D001120:Arginine; D002222:Carbamoyl-Phosphate Synthase (Ammonia)