title: Performance characteristics of four automated natriuretic peptide assays.
abstract: Measurement of circulating B-type natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) can identify patients with heart failure and guide therapy. The limit of detection, linearity, imprecision, method comparison, analytic concordance, and reference intervals of the Access 2 BNP (Biosite, San Diego, CA), ADVIA Centaur BNP (Bayer Diagnostics, Tarrytown, NY), AxSYM BNP (Abbott Diagnostics, Abbott Park, IL), and E170 NT-proBNP (Roche Diagnostics, Indianapolis, IN) methods were evaluated. The Triage meter BNP assay (Biosite) was the comparison method. Imprecision testing showed total coefficients of variation of 4.1%, 4.4%, 5.5%, and 0.8% for the Access 2, ADVIA Centaur, AxSYM, and E170, respectively. Relative to the Triage meter, method comparison revealed a slope of 0.96 and r = 0.95, a slope of 0.77 and r = 0.92, a slope of 1.13 and r = 0.94, and a slope of 8.8 and r = 0.80 for the Access 2, ADVIA Centaur, AxSYM, and E170, respectively. Overall analytic concordance values with the Triage meter were 95.9%, 92.9%, 92.4%, and 84.3% for the Access 2, ADVIA Centaur, AxSYM, and E170, respectively. All automated natriuretic peptide methods showed acceptable analytic performance.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA.
authors.forename: Mindy L
authors.initials: ML
authors.lastname: Rawlins
authors.forename: William E
authors.initials: WE
authors.lastname: Owen
authors.forename: William L
authors.initials: WL
authors.lastname: Roberts
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation / Q000706:statistics & numerical data; D002624:Chemistry, Clinical / Q000295:instrumentation* / Q000706:statistics & numerical data; D006801:Humans; D016014:Linear Models; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D009419:Nerve Tissue Proteins / Q000097:blood*; D011498:Protein Precursors / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D009419:Nerve Tissue Proteins; D011498:Protein Precursors; C063382:prepro-brain natriuretic peptide; D020097:Natriuretic Peptide, Brain