title: Hyper-homocysteinemia is not a main feature of juvenile uncomplicated type 1 diabetes.
abstract: Total plasma homocysteine (tHcy) was measured by high pressure liquid chromatography (HPLC) method in 28 patients (12 females and 16 males) at the onset of type 1 diabetes mellitus (T1DM), 4 females during diabetes ketoacidosis (DKA) and 154 (68 females and 86 males) during follow-up. Serum folate, pyridoxal 5' phosphate (PLP) and Vitamin B12 (Vit B12) were also measured. Plasma tHcy levels were not found significantly different in T1DM patients known to have diabetes (males 9.2 +/- 7.7 and females 7.0 +/- 2.8 micromol/l) and in those who were newly diagnosed (males 9.7 +/- 4.8 and females 7.16 +/- 2.8 micromol/l) than in healthy controls (males 8.7 +/- 3.5 and females 7.8 +/- 2.55 micromol/l). Only a significant difference for sex was observed in known diabetes (p = 0.0281). Serum folate, PLP and Vit B12 were normal (12.6 +/- 3.6 ng/ml, 20.11 +/- 0.8 ng/ml and 416.7 +/- 41.9 pg/ml) in all T1DM patients. Age significantly correlated with plasma tHcy. Only in 4 patients, studied during DKA, plasma tHcy was significantly lower (2.76 +/- 1.33 micromol/l, p < 0.001) than the healthy controls.
authors.affiliation: Department of Pharmacology, Ginecology and Obstetrics, Pediatrics, University of Sassari, Sassari, Italy.
authors.forename: Gian Franco
authors.initials: GF
authors.lastname: Meloni
authors.forename: Gian Carlo
authors.initials: GC
authors.lastname: Tonolo
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zuppi
authors.forename: Bruno
authors.initials: B
authors.lastname: Zappacosta
authors.forename: Salvatore
authors.initials: S
authors.lastname: Musumeci
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003922:Diabetes Mellitus, Type 1 / Q000150:complications*; D005260:Female; D006710:Homocysteine / Q000097:blood; D006801:Humans; D020138:Hyperhomocysteinemia / Q000150:complications*; D008297:Male
chemical_list: D006710:Homocysteine