title: Improved anti-inflammatory and pharmacokinetic properties for superoxide dismutase by chemical glycosidation with carboxymethylchitin.
abstract: O-carboxymethylchitin (molecular weight = 1.07 x 10(5), degree of carboxymethylation = 80%, degree of N-acetylation = 91%) was chemically attached to superoxide dismutase by the formation of amide linkages through a carbodiimide catalyzed reaction. The glycosidated enzyme contained about 1.8 mole of polysaccharide per mole of protein and retained 57% of the initial catalytic activity. The anti-inflammatory activity of the enzyme was 2.4 times increased after conjugation with the polysaccharide. The modified superoxide dismutase preparation was remarkably more resistant to inactivation with H(2)O(2) and its plasma half-life time was prolonged from 4.8 min to 69 h.
authors.affiliation: Enzyme Technology Group, Center for Biotechnological Studies, University of Matanzas, Autopista a Varadero Km 3 1/2, Matanzas, C.P. 44740, Cuba.
authors.forename: Aimara
authors.initials: A
authors.lastname: Valdivia
authors.forename: Yunel
authors.initials: Y
authors.lastname: Perez
authors.forename: Amalia
authors.initials: A
authors.lastname: Dominguez
authors.forename: Julio
authors.initials: J
authors.lastname: Caballero
authors.forename: Leissy
authors.initials: L
authors.lastname: Gomez
authors.forename: Etienne H
authors.initials: EH
authors.lastname: Schacht
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism*; D004487:Edema / Q000188:drug therapy; D005121:Extremities; D006031:Glycosylation; D051381:Rats; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D002686:Chitin; C044872:O-(carboxymethyl)chitin; D013482:Superoxide Dismutase