title: Solution structure of an RNA internal loop with three consecutive sheared GA pairs.
abstract: Internal loops in RNA are important for folding and function. Many folding motifs are internal loops containing GA base pairs, which are usually thermodynamically stabilizing, i.e., contribute favorable free energy to folding. Understanding the sequence dependence of folding stability and structure in terms of molecular interactions, such as hydrogen bonding and base stacking, will provide a foundation for predicting stability and structure. Here, we report the NMR structure of the oligonucleotide duplex, 5'GGUGGAGGCU3'/3'PCCGAAGCCG5' (P = purine), containing an unusually stable and relatively abundant internal loop, 5'GGA3'/3'AAG5'. This loop contains three consecutive sheared GA pairs (trans Hoogsteen/Sugar edge AG) with separate stacks of three G's and three A's in a row. The thermodynamic consequences of various nucleotide substitutions are also reported. Significant destabilization of approximately 2 kcal/mol at 37 degrees C is found for substitution of the middle GA with AA to form 5'GAA3'/3'AAG5'. This destabilization correlates with a unique base stacking and hydrogen-bonding network within the 5'GGA3'/3'AAG5' loop. Interestingly, the motifs, 5'UG3'/3'GA5' and 5'UG3'/3'AA5', have stability similar to 5'CG3'/3'GA5' even though UG and UA pairs are usually less stable than CG pairs. Consecutive sheared GA pairs in the 5'GGA3'/3'AAG5' loop are preorganized for potential tertiary interactions and ligand binding.
authors.affiliation: Department of Chemistry, University of Rochester, Rochester, New York 14627, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Brent M
authors.initials: BM
authors.lastname: Znosko
authors.forename: Scott D
authors.initials: SD
authors.lastname: Kennedy
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Krugh
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Turner
mesh_terms: D000225:Adenine; D020029:Base Pairing; D001483:Base Sequence; D006147:Guanine; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D012996:Solutions; D013816:Thermodynamics
chemical_list: D009843:Oligoribonucleotides; D012996:Solutions; D006147:Guanine; D012313:RNA; D000225:Adenine