title: Polyunsaturated fatty acids interact with the PPARA-L162V polymorphism to affect plasma triglyceride and apolipoprotein C-III concentrations in the Framingham Heart Study.
abstract: Peroxisome proliferator-activated receptor alpha (PPARalpha) is a nuclear transcription factor regulating multiple genes involved in lipid metabolism. It was shown that a common leucine to valine (L162V) substitution at the PPARalpha gene (PPARA) is functional and affects transactivation activity of PPARalpha ligands, such as PUFA, on a concentration-dependent basis. The current study examined this gene-nutrient interaction in relation to plasma lipid variables in a population-based study consisting of 1003 men and 1103 women participating in the Framingham cohort and consuming their habitual diets. We found significant gene-nutrient interactions between the L162V polymorphism and total PUFA intake, which modulated plasma triglycerides (TG; P < 0.05) and apolipoprotein C-III (apoC-III; P < 0.05) concentrations. The 162V allele was associated with greater TG and apoC-III concentrations only in subjects consuming a low-PUFA diet (below the population mean, 6% of energy). However, when PUFA intake was high, carriers of the 162V allele had lower apoC-III concentrations. This interaction was significant even when PUFA intake was considered as a continuous variable (P = 0.031 for TG and P < 0.001 for apoC-III), suggesting a strong dose-response effect. When PUFA intake was <4%, 162V allele carriers had approximately 28% higher plasma TG than did 162L homozygotes (P < 0.01). Conversely, when PUFA intake was >8%, plasma TG in 162V allele carriers was 4% lower than in 162L homozygotes. Similar results were obtained for (n-6) and (n-3) fatty acids. Our data show that the effect of the L162V polymorphism on plasma TG and apoC-III concentrations depends on the dietary PUFA, with a high intake triggering lower TG in carriers of the 162V allele.
authors.affiliation: Nutrition and Genomics Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111-1524, USA.
authors.forename: E Shyong
authors.initials: ES
authors.lastname: Tai
authors.forename: Dolores
authors.initials: D
authors.lastname: Corella
authors.forename: Serkalem
authors.initials: S
authors.lastname: Demissie
authors.forename: L Adrienne
authors.initials: LA
authors.lastname: Cupples
authors.forename: Oscar
authors.initials: O
authors.lastname: Coltell
authors.forename: Ernst J
authors.initials: EJ
authors.lastname: Schaefer
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Tucker
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovas
mesh_terms: D000428:Alcohol Drinking; D019943:Amino Acid Substitution; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000097:blood*; D016022:Case-Control Studies; D003920:Diabetes Mellitus / Q000097:blood; D004041:Dietary Fats; D002149:Energy Intake; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D047493:PPAR alpha / Q000235:genetics* / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D012727:Sex Characteristics; D012907:Smoking; D014280:Triglycerides / Q000097:blood*
chemical_list: D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D047493:PPAR alpha; D014280:Triglycerides