[Effect of gardenin on gene expression profile in brain of rats with focal cerebral ischemia].
OBJECTIVE
To explore the pharmacologic mechanism of gardenin in treating cerebral ischemia, by studying its effect on gene expression profile in brain of rats with focal cerebral ischemia (FCI).


METHODS
Total RNAs were isolated from rats with FCI and those treated with gardenin. The mRNAs were reversely transcribed to cDNA with incorporation of fluorescent Cy5- or Cy3-dUTP to prepare hybridization probes. The PCR products of 4096 genes were spotted on the chip after a serial treatment. The mixed probes were hybridized to the cDNA microarray. Axon Genepix 4000B and GenePixPro 3.0 software were used to scan and analyze the fluorescent signals.


RESULTS
In the group treated with gardenin, there were 70 genes had expression profiles different to that in the model group in the focal cerebral ischemic brain tissue, in which 68 were up-regulated and 2 down-regulated.


CONCLUSIONS
Gardenin has regulatory effect on the gene expression in rats with focal cerebral ischemia, which elucidates part of the pharmacologic mechanism of Qingkailing in molecular level.
Zhang
Xiao-yan
XY
Institute of Life Science, Beijing Normal University, Beijing 100875. xiaoyanzhang@hotmail.com
Zhang
Zhan-jun
ZJ
Wang
Zhong
Z
Ying
Kang
K
Zuo
Ming-xue
MX
D000818:Animals; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D011725:Pyridines; C074986:gardenine