title: [The establishment and clinical application of multicolor fluorescence in situ hybridization].
abstract: OBJECTIVE
  To establish a rapid and convenient method of multi-color fluorescence in situ hybridization (FISH) on several different tissue samples: peripheral blood samples, amniotic fluid, embryos and bone marrow samples.
  
  
  METHODS
  FISH analysis was carried out on different tissue samples, using probes specific for chromosomes 13,18,21,X and Y or for BCR/ABL gene.
  
  
  RESULTS
  FISH analysis could reveal hybridization signals on metaphase chromosomes and interphase nuclei, it is also possible to detect chromosome aberrations.
  
  
  CONCLUSIONS
  Multi-color FISH is clinically useful tool, which can be used as an adjunct to conventional chromosome analysis for prenatal diagnosis , preimplantation genetic diagnosis and diagnosis of leukemia.
authors.affiliation: Guangzhou Second People's Hospital, Guangzhou Research Institute of Obstetrics and Gynecology, Guangzhou 510150, China. qinglicn@sina.com.cn
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Sun
authors.forename: Xin-jie
authors.initials: XJ
authors.lastname: Chen
authors.forename: Shu
authors.initials: S
authors.lastname: Kong
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Zheng
authors.forename: Yan-yi
authors.initials: YY
authors.lastname: Huang
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*