title: Inhibitory constituents against cyclooxygenases from Aralia cordata Thunb.
abstract: Seven diterpenes, four polyacetylenes, a lipid glycerol, and two sterols were isolated from the methylene chloride fraction of the root of Aralia cordata. Their chemical structures were determined as (-)-pimara-8(14),15-dien-19-oic acid (2), pimaric acid (3), (-)-kaur-16-en-19-oic acid (4), 17-hydroxy-ent-kaur-15-en-19-oic acid (9), 7alpha-hydroxy-(-)-pimara-8(14),15-dien-19-oic acid (10), 16alpha,17-dihydroxy-(-)-kauran-19-oic acid (11), 16-hydroxy-17-isovaleroyloxy-ent-kauran-19-oic acid (12), falcarindiol (5), dehydrofalcarindiol (6), dehydrofalcarindiol-8-acetate (7), falcarindiol-8-acetate (8), alpha-mono palmitin (13), stigmasterol (1), and daucosterol (14) by the spectral evidences. These compounds were tested with COX-1 and COX-2 inhibition assays. This study found that compounds 2, 4, 5, 6, 7, 8, and 10 inhibited COX-1 dependent conversion of the exogenous arachidonic acid to PGE2 in a dose-dependent manner with IC50 values of 134.2 microM, 121.6 microM, 170 microM, 50.4 microM, 11.7 microM, 99.6 microM, and 69.6 microM, respectively. But, most of these compounds weakly inhibited COX-2 dependent PGE2 generation. Among them, only compound 4 showed relatively significant inhibitory activity (IC50: 127.6 microM).
authors.affiliation: College of Pharmacy, Chungnam National University, Taejon 305-764, Korea.
authors.forename: Nguyen Hai
authors.initials: NH
authors.lastname: Dang
authors.forename: XinFeng
authors.initials: X
authors.lastname: Zhang
authors.forename: MingShan
authors.initials: M
authors.lastname: Zheng
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
authors.forename: Hyun Pyo
authors.initials: HP
authors.lastname: Kim
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
mesh_terms: D027983:Aralia; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*
chemical_list: D016861:Cyclooxygenase Inhibitors; D010936:Plant Extracts; D011451:Prostaglandin-Endoperoxide Synthases