A carbohydrate fraction, AIP1, from Artemisia iwayomogi down-regulates Fas gene expression and suppresses apoptotic death of the thymocytes induced by 2,3,7,8-tectrachlorodibenzo-p-dioxin.
Apoptotic death of mouse thymocytes in vitro, as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), involves the up-regulation of Fas gene expression, while a carbohydrate fraction, AIP1, from Artemisia iwayomogi suppresses the death of thymocytes in culture along with the down-regulation of Fas gene expression. We have now investigated whether the AIP1 fraction modulates TCDD-induced thymocyte death. When treated with TCDD and AIP1 fraction together, the thymocytes do not show apoptosis induced by the TCDD treatment. The AIP1 supplementation to the TCDD treatment also down-regulates the TCDD-induced Fas gene up-regulation. These findings indicate that the AIP1 fraction suppresses TCDD-induced thymocyte apoptosis through the modulation of Fas gene expression.
Ji
Hee Jung
HJ
Department of Microbiology, School of Biotechnology & Biomedical Science and Bio-Health Product Research Center, Inje University, Gimhae, Gyungnam, 621-749, S. Korea.
Yeo
Hee Kyoung
HK
Lee
Nam Hee
NH
Hwang
Jung Suk
JS
Koo
Kyung Ah
KA
Cheong
Seon Woo
SW
Park
Joo Hung
JH
Oh
Gap Soo
GS
Yoon
Chun Sik
CS
Youn
Hyun Joo
HJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D019306:Artemisia / Q000378:metabolism*; D002241:Carbohydrates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004147:Dioxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004347:Drug Interactions; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism*; D019014:fas Receptor
D002241:Carbohydrates; D004147:Dioxins; C491899:Fas protein, mouse; D018124:Receptors, Tumor Necrosis Factor; D019014:fas Receptor; C066841:2,3,7,8-tetrabromodibenzo-4-dioxin