title: Dietary exposure to Aroclor 1254 alters gene expression in Xenopus laevis frogs.
abstract: Polychlorinated biphenyls (PCBs) are persistent environmental pollutants that contribute to worldwide health problems. Despite data associating PCBs with adverse health effects, decisions to clean up contaminated sites remain controversial. Cleanup decisions are typically based on risk assessment methods that are not sensitive enough to detect subtle changes in health. We have recently shown that gene expression signatures can serve as sensitive molecular biomarkers of exposure and related health effects. Our initial studies were carried out with developing Xenopus laevis tadpoles that were exposed to the PCB mixture Aroclor 1254 (A1254) for 2 days. A1254 was dissolved in dimethyl sulfoxide and added to the aquarium water for rapid loading of PCBs into the tadpole tissue. These studies showed that increases in the expression of specific genes occurred independent of adverse health effects, and decreases in specific genes correlated with the appearance of observable health effects, including decreased survival and gross morphological and behavioral abnormalities. In this report, we extend our previous work to test the use of gene expression signatures as biomarkers in frogs exposed to PCBs through the diet from early tadpole stages through metamorphosis. This work showed that chronic low-dose exposure to A1254 (24 ppm) in food produced tissue levels of 17 ppm and increased gene expression of nerve growth factor and proopiomelanocortin independent of adverse health effects. Exposure to higher doses of A1254 (200 ppm) produced tissue levels of 80 ppm and increased expression of p450 1A1, also, independent of adverse health effects. This work provides further evidence for the use of gene expression changes as biomarkers of exposure to PCBs.
authors.affiliation: Environmental Institute and the Great Lakes Center for Environmental and Molecular Sciences, Western Michigan University, Room 3924 Wood Hall, Kalamazoo, MI 49008, USA. anna.jelaso@wmich.edu
authors.forename: Anna M
authors.initials: AM
authors.lastname: Jelaso
authors.forename: Cari
authors.initials: C
authors.lastname: DeLong
authors.forename: Jay
authors.initials: J
authors.lastname: Means
authors.forename: Charles F
authors.initials: CF
authors.lastname: Ide
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D020170:Caspase 1 / Q000096:biosynthesis / Q000235:genetics; D020111:Chlorodiphenyl (54% Chlorine) / Q000493:pharmacokinetics / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000096:biosynthesis / Q000235:genetics; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008675:Metamorphosis, Biological / Q000187:drug effects* / Q000235:genetics; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014982:Xenopus laevis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D000199:Actins; D017448:Receptors, Dopamine D2; D020111:Chlorodiphenyl (54% Chlorine); D011333:Pro-Opiomelanocortin; D020932:Nerve Growth Factor; D019363:Cytochrome P-450 CYP1A1; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D020170:Caspase 1