title: Temporal separation of replication and recombination requires the intra-S checkpoint.
abstract: In response to DNA damage and replication pausing, eukaryotes activate checkpoint pathways that prevent genomic instability by coordinating cell cycle progression with DNA repair. The intra-S-phase checkpoint has been proposed to protect stalled replication forks from pathological rearrangements that could result from unscheduled recombination. On the other hand, recombination may be needed to cope with either stalled forks or double-strand breaks resulting from hydroxyurea treatment. We have exploited fission yeast to elucidate the relationship between replication fork stalling, loading of replication and recombination proteins onto DNA, and the intra-S checkpoint. Here, we show that a functional recombination machinery is not essential for recovery from replication fork arrest and instead can lead to nonfunctional fork structures. We find that Rad22-containing foci are rare in S-phase cells, but peak in G2 phase cells after a perturbed S phase. Importantly, we find that the intra-S checkpoint is necessary to avoid aberrant strand-exchange events during a hydroxyurea block.
authors.affiliation: CNRS UMR2027, Institut Curie, Centre Universitaire, 91405 Orsay Cedex, France.
authors.forename: Peter
authors.initials: P
authors.lastname: Meister
authors.forename: Angela
authors.initials: A
authors.lastname: Taddei
authors.forename: Laurence
authors.initials: L
authors.lastname: Vernis
authors.forename: Mickaël
authors.initials: M
authors.lastname: Poidevin
authors.forename: Susan M
authors.initials: SM
authors.lastname: Gasser
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Baldacci
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D004260:DNA Repair / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D016195:G2 Phase / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D042822:Genomic Instability / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006918:Hydroxyurea / Q000633:toxicity; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics*; D016196:S Phase / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; C085412:rad52 protein, S pombe; D006918:Hydroxyurea
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