title: [Estimation of detection limit from slope of B/B0 in competitive ELISA].
abstract: A method has been proposed for determining the detection limit (L(D)) from the slope of a semilogarithmic plot of a B/B(0) curve in competitive enzyme linked immunosorbent assay (ELISA). As an application, this paper describes a graphic determination of L(D) from analogue data in the literature. The L(D) obtained corresponds to the concentration at which the relative standard deviation of concentration estimates is 30%.
authors.affiliation: National Institute of Health Sciences, Tokyo 158-8501, Japan. fumi@nihs.go.jp
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Rieko
authors.initials: R
authors.lastname: Matsuda
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ito
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Imai
authors.forename: Masako
authors.initials: M
authors.lastname: Maeda
mesh_terms: D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D016015:Logistic Models; D012680:Sensitivity and Specificity