title: Monokaryotic chloroplast mutation has no effect on non-Mendelian transmission of chloroplast and mitochondrial DNA in Chlamydomonas species.
abstract: We studied whether the monokaryotic chloroplast (moc) mutation affects the transmission of chloroplast and mitochondrial DNA in Chlamydomonas species. We used a previously isolated moc mutant from our cell line G33, which had only one large chloroplast nucleus. To obtain zygotes, we crossed the mutant cells with wild-type cells, and mutant cells with receptive mates (females [mt+] with males [mt-]). In these zygotes, we recorded preferential dissolution of mt- parental chloroplast nuclei and fusion of the two cell nuclei. Antibiotic-resistance markers of chloroplast DNA were maternally transmitted in all crosses. PCR analysis of the cytochrome b (cob) gene sequence showed that the mitochondrial DNA was paternally transmitted to offspring. These results suggest that the moc mutation did not affect the organelle DNA transmission.
authors.affiliation: Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, Japan. nsoichi@sci.u-ryukyu.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: O
authors.initials: O
authors.lastname: Misumi
authors.forename: H
authors.initials: H
authors.lastname: Aoyama
authors.forename: R
authors.initials: R
authors.lastname: van Woesik
authors.forename: T
authors.initials: T
authors.lastname: Kuroiwa
mesh_terms: D000818:Animals; D002696:Chlamydomonas / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D003433:Crosses, Genetic; D018742:DNA, Chloroplast / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005111:Extrachromosomal Inheritance; D005260:Female; D008297:Male; D009154:Mutation
chemical_list: D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial