Comparison of chemopreventive effects of Vitamin E plus selenium versus melatonin in 7,12-dimethylbenz(a)anthracene-induced mouse brain damage.
In this work, the protective effect of Vitamin E plus selenium (Vit E+Se) and melatonin against 7,12-dimethylbenz(a)anthracene (7,12-DMBA)-induced changes in superoxide dismutase (SOD), glutathione peroxidase (GSHPx), catalase (CAT) and carbonic anhydrase (CA) activities and malonedialdehyde (MDA) levels of mouse brain were compared. 12-month old mice were divided into four groups each including 10 animals. The first group served as control group. The second group was treated with 7,12-DMBA (20 mg/(kg day)). The third group was treated with 7,12-DMBA and Vitamin E (90 microg/(individual day)) and selenium (1.8 microg/(individual day)) simultaneously. The fourth group was treated with 7,12-DMBA and melatonin (4.2 mg/(kg day)) simultaneously. Treatment continued for 21 days after which the mice were sacrificed and brain homogenates were prepared. 7,12-DMBA treated group exhibited significantly decreased levels of brain SOD, GSHPx, CAT and CA activities and increased MDA levels as compared to control. Vitamin E+Se fully or partially restored enzyme inhibition except for SOD. Lipid peroxidation was also reduced in Vitamin E+Se treated group. Melatonin provided a better protection for SOD, GSHPx and CAT, and a plausible protection for CA activity. Protection against lipid peroxidation measured as MDA in melatonin treated group was appreciable although slightly lesser than the protection provided by Vitamin E+Se. The results imply that Vitamin E+Se and melatonin both provide chemoprevention against 7,12-DMBA-induced oxidative stress in mouse brain.
Batcioglu
Kadir
K
Biochemistry Department, Faculty of Pharmacy, Inonu University, Malatya, Turkey. kbatcioglu@inonu.edu.tr
Karagözler
Arife Alev
AA
Ozturk
Ismail Cetin
IC
Genc
Metin
M
Bay
Aysun
A
Ozturk
Feral
F
Aydogdu
Nurettin
N
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology / Q000473:pathology*; D002256:Carbonic Anhydrases / Q000494:pharmacology; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D002374:Catalase / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D005979:Glutathione Peroxidase / Q000494:pharmacology; D008315:Malondialdehyde / Q000032:analysis; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D018384:Oxidative Stress; D012643:Selenium / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D002273:Carcinogens; D014810:Vitamin E; D008315:Malondialdehyde; D015127:9,10-Dimethyl-1,2-benzanthracene; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases; D012643:Selenium; D008550:Melatonin