Separating human DNA mixtures using denaturing high-performance liquid chromatography.
DNA mixtures represent challenging samples that are rarely amenable to direct DNA sequence analysis and many of the strategies available to separate mixtures are both labor and time intensive. Denaturing high-performance liquid chromatography is an accurate and rapid approach for the detection and scoring of mutations. It can also be used to separate DNA mixtures. The technique relies on the chromatographic separation of crosshybridization products to isolate the individual components of a mixture. By eliminating secondary amplification and excessive manipulation prior to sequencing, denaturing high-performance liquid chromatography can streamline the analysis of conditions ranging from somatic mosaicism, microchimerism and mitochondrial heteroplasmy to evidentiary material containing mixtures of DNA encountered in forensic investigations.
Danielson
Phillip B
PB
University of Denver, Department of Biological Sciences, Denver, CO 80210, USA. pdaniels@du.edu
Kristinsson
Richard
R
Shelton
Robert J
RJ
Laberge
Greggory S
GS
D046528:Chimerism; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000302:isolation & purification*; D004272:DNA, Mitochondrial / Q000302:isolation & purification; D005819:Genetic Markers; D006801:Humans; D009030:Mosaicism; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D005819:Genetic Markers; D004247:DNA