Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli.
Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells to operate at the multicellular level. Many environmental stimuli have been shown to affect the extracellular AI-2 levels, carbon sources being among the most important. In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression. Specifically, cAMP-CRP is shown to bind to a CRP binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake. The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes.
Wang
Liang
L
Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA.
Hashimoto
Yoshifumi
Y
Tsao
Chen-Yu
CY
Valdes
James J
JJ
Bentley
William E
WE
D001426:Bacterial Proteins / Q000235:genetics; D013437:Carbon-Sulfur Lyases; D000242:Cyclic AMP / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism*; D007783:Lactones / Q000378:metabolism*; D016296:Mutagenesis; D009699:N-Glycosyl Hydrolases / Q000235:genetics; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D011956:Receptors, Cell Surface; D011953:Receptors, Cyclic AMP; D014157:Transcription Factors; C481662:crp protein, E coli; D006714:Homoserine; D000242:Cyclic AMP; D009699:N-Glycosyl Hydrolases; C019606:adenosylhomocysteine nucleosidase; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria; D005947:Glucose
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