Metabolites of conjugated isomers of alpha-linolenic acid (CLnA) in the rat.
Rumelenic (cis-9,trans-11,cis-15 18:3) acid is a naturally occurring conjugated isomer of alpha-linolenic acid (CLnA) in milk fat. Metabolism in rats was studied using a synthetic CLnA mixture, composed mainly by equimolar quantities of cis-9,trans-11,cis-15 and cis-9,trans-13,cis-15 CLnA isomers. Their metabolisms were studied by feeding high quantities of CLnA (150 mg/day) for 4 days to rats that had been reared on a fatfree diet for 2 weeks. After this period, animals were sacrificed and liver and epididymal adipose tissue lipids extracted. Six metabolites of the cis-9,trans-11,cis-15 18:3 CLnA isomers were identified as being cis-7,trans-9,cis-13 16:3, cis-11,trans-13,cis-17 20:3, cis-8,cis-11,trans-13,cis-17 20:4, cis-5,cis-8,cis-11,trans-13,cis-17 20:5, cis-7,cis-10,cis-13,trans-15,cis-19 22:5, and cis-4,cis-7,cis-10,cis-13,trans-15,cis-19 22:6 acids. Two metabolites of cis-9,trans-13,cis-15 18:3 CLnA isomer were also identified by GC-MS as being cis-7,trans-11,cis-13 16:3 and cis-5,cis-8,cis-11,trans-15,cis-17 20:5.
Destaillats
Frédéric
F
Department of Food Science and Nutrition and Dairy Research Center (STELA), Université Laval, Sainte Foy, Québec, Canada.
Berdeaux
Olivier
O
Sébédio
Jean-Louis
JL
Juaneda
Pierre
P
Grégoire
Stéphane
S
Chardigny
Jean-Michel
JM
Bretillon
Lionel
L
Angers
Paul
P
D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D017962:alpha-Linolenic Acid / Q000737:chemistry / Q000378:metabolism*
D004041:Dietary Fats; D017962:alpha-Linolenic Acid