title: 53BP2 induces apoptosis through the mitochondrial death pathway.
abstract: The p53 binding protein 2 (53BP2) has been identified as the interacting protein to p53, Bcl-2, and p65 subunit of nuclear factor kappaB (NF-kappaB). The TP53BP2 gene encodes two splicing variants, 53BP2S and 53BP2L, previously known as apoptosis stimulating protein 2 of p53 (ASPP2). We found that these 53BP2 proteins are located predominantly in the cytoplasm and induce apoptosis as demonstrated by cleavage of poly ADP ribose polymerase (PARP) and annexin V staining. Furthermore, we demonstrate that 53BP2 is located in the mitochondria and induces apoptosis associated with depression of the mitochondrial trans-membrane potential (DeltaPsim) and activation of caspase-9. From these findings we conclude that 53BP2 induces apoptosis through the mitochondrial death pathway.
authors.affiliation: Department of Molecular and Cellular Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kajino
authors.forename: Naoko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kanazawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Imai
authors.forename: Yurina
authors.initials: Y
authors.lastname: Hibi
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ohara
authors.forename: Makoto
authors.initials: M
authors.lastname: Itoh
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamoto
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004441:Ecdysterone / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; C090468:TP53BP2 protein, human; D004441:Ecdysterone; C008999:ponasterone A; C506433:CASP9 protein, human; D053453:Caspase 9; D020169:Caspases