Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system.
Non-O1, non-O139 Vibrio cholerae can cause gastroenteritis and extraintestinal infections, but, unlike O1 and O139 strains of V. cholerae, little is known about the virulence gene content of non-O1, non-O139 strains and their phylogenetic relationship to other pathogenic V. cholerae. Comparative genomic microarray analysis of four pathogenic non-O1, non-O139 strains indicates that these strains are quite divergent from O1 and O139 strains. Genomic sequence analysis of a non-O1, non-O139 strain (AM-19226) that appeared particularly pathogenic in experimental animals suggests that this strain carries a type III secretion system (TTSS) that is related to the TTSS2 gene cluster found in a pandemic clone of Vibrio parahaemolyticus. The genes for this V. cholerae TTSS system appear to be present in many clinical and environmental non-O1, non-O139 strains, including at least one clone that is globally distributed. We hypothesize that the TTSS present in some pathogenic strains of non-O1, non-O139 V. cholerae may be involved in the virulence and environmental fitness of these strains.
Dziejman
Michelle
M
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Serruto
Davide
D
Tam
Vincent C
VC
Sturtevant
Derek
D
Diraphat
Pornphan
P
Faruque
Shah M
SM
Rahman
M Hasibur
MH
Heidelberg
John F
JF
Decker
Jeremy
J
Li
Li
L
Montgomery
Kate T
KT
Grills
George
G
Kucherlapati
Raju
R
Mekalanos
John J
JJ
D000818:Animals; D015139:Blotting, Southern; D003967:Diarrhea / Q000382:microbiology; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023421:Models, Animal; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D011817:Rabbits; D014734:Vibrio cholerae / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
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