title: Salicylic acid-dependent expression of host genes in compatible Arabidopsis-virus interactions.
abstract: Plant viruses elicit the expression of common sets of genes in susceptible hosts. Studies in Arabidopsis (Arabidopsis thaliana) and tomato (Lycopersicon esculentum) indicate that at least one-third of the genes induced in common by viruses have been previously associated with plant defense and stress responses. The genetic and molecular requirements for the induction of these stress and defense-related genes during compatible host-virus interactions were investigated with a panel of Arabidopsis mutant and transgenic plants defective in one or more defense signaling pathways. pad4, eds5, NahG, npr1, jar1, ein2, sid2, eds1, and wild-type Columbia-0 and Wassilewskija-2 plants were infected with two different viruses, cucumber mosaic virus and oilseed rape mosaic virus. Gene expression was assayed by a high-throughput fiber-optic bead array consisting of 388 genes and by RNA gel blots. These analyses demonstrated that, in compatible host-virus interactions, the expression of the majority of defense-related genes is induced by a salicylic acid-dependent, NPR1-independent signaling pathway with a few notable exceptions that did require NPR1. Interestingly, none of the mutant or transgenic plants showed enhanced susceptibility to either cucumber mosaic virus or oilseed rape mosaic virus based on both symptoms and virus accumulation. This observation is in contrast to the enhanced disease susceptibility phenotypes that these mutations or transgenes confer to some bacterial and fungal pathogens. These experimental results suggest that expression of many defense-related genes in compatible host plants might share components of signaling pathways involved in incompatible host-pathogen interactions, but their increased expression has no negative effect on viral infection.
authors.affiliation: Department of Plant Pathology, Iowa State University, Ames, Iowa 50011-1020, USA.
authors.forename: Zhonglian
authors.initials: Z
authors.lastname: Huang
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Yeakley
authors.forename: Elizabeth Wickham
authors.initials: EW
authors.lastname: Garcia
authors.forename: Jaime D
authors.initials: JD
authors.lastname: Holdridge
authors.forename: Jian-Bing
authors.initials: JB
authors.lastname: Fan
authors.forename: Steven A
authors.initials: SA
authors.lastname: Whitham
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D009029:Mosaic Viruses / Q000502:physiology*; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D030821:Plants, Genetically Modified; D012367:RNA, Viral / Q000502:physiology; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D012367:RNA, Viral; D020156:Salicylic Acid
references.citation: Bioinformatics. 2003 Sep 22;19(14):1832-6
references.pmid: 14512355
references.citation: Plant Cell. 2001 Jul;13(7):1499-510
references.pmid: 11449047
references.citation: Science. 1995 Jan 13;267(5195):229-31
references.pmid: 17791345
references.citation: Cold Spring Harb Symp Quant Biol. 2003;68:69-78
references.pmid: 15338605
references.citation: Plant Cell. 1997 Apr;9(4):547-557
references.pmid: 12237364
references.citation: Mol Plant Microbe Interact. 1997 Jan;10(1):69-78
references.pmid: 9002272
references.citation: Plant J. 2001 May;26(4):395-407
references.pmid: 11439127
references.citation: Plant Cell. 1999 Aug;11(8):1393-404
references.pmid: 10449575
references.citation: J Comput Biol. 2001;8(6):625-37
references.pmid: 11747616
references.citation: Plant Cell. 2004 May;16(5):1302-13
references.pmid: 15100397
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
references.pmid: 9843981
references.citation: Curr Opin Plant Biol. 2001 Aug;4(4):301-8
references.pmid: 11418339
references.citation: Curr Opin Plant Biol. 2002 Aug;5(4):325-31
references.pmid: 12179966
references.citation: Mol Plant Microbe Interact. 2000 Apr;13(4):430-8
references.pmid: 10755306
references.citation: Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15289-93
references.pmid: 8986804
references.citation: Plant Cell. 2002 Jun;14(6):1377-89
references.pmid: 12084833
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3298-303
references.pmid: 14960727
references.citation: Plant Cell. 2004 Feb;16(2):319-31
references.pmid: 14742872
references.citation: Plant J. 2000 Oct;24(2):205-18
references.pmid: 11069695
references.citation: Curr Opin Plant Biol. 2004 Aug;7(4):365-71
references.pmid: 15231257
references.citation: Cell. 2000 Dec 22;103(7):1111-20
references.pmid: 11163186
references.citation: Plant Cell. 1996 Dec;8(12):2309-23
references.pmid: 8989885
references.citation: Genome Res. 2004 May;14(5):878-85
references.pmid: 15123585
references.citation: Plant Cell. 2000 Nov;12(11):2175-90
references.pmid: 11090217
references.citation: Mol Plant Microbe Interact. 2002 Oct;15(10):990-9
references.pmid: 12437296
references.citation: Plant Cell. 1998 Apr;10(4):557-69
references.pmid: 9548982
references.citation: Plant Cell. 2004 May;16(5):1235-50
references.pmid: 15084715
references.citation: Plant Cell. 1996 Feb;8(2):241-9
references.pmid: 8742710
references.citation: Genome Biol. 2002 Aug 30;3(9):research0048
references.pmid: 12225587
references.citation: Dev Cell. 2003 Feb;4(2):205-17
references.pmid: 12586064
references.citation: Nature. 2001 Nov 29;414(6863):562-5
references.pmid: 11734859
references.citation: Plant J. 2002 Dec;32(5):655-67
references.pmid: 12472683
references.citation: Plant Cell. 2000 Dec;12(12):2339-2350
references.pmid: 11148282
references.citation: Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):15228-33
references.pmid: 12403829
references.citation: Science. 1993 Aug 6;261(5122):754-6
references.pmid: 17757215
references.citation: Am J Pathol. 2004 Nov;165(5):1799-807
references.pmid: 15509548
references.citation: Genetics. 1996 Jun;143(2):973-82
references.pmid: 8725243
references.citation: Mol Plant Microbe Interact. 2003 Aug;16(8):681-8
references.pmid: 12906112
references.citation: Genes Dev. 2004 May 15;18(10):1179-86
references.pmid: 15131083
references.citation: Trends Genet. 2001 Aug;17(8):449-59
references.pmid: 11485817
references.citation: Plant Cell. 2000 May;12(5):677-90
references.pmid: 10810143
references.citation: Plant Cell. 2000 Oct;12(10):1975-86
references.pmid: 11041891
references.citation: Curr Opin Plant Biol. 2002 Aug;5(4):279-84
references.pmid: 12179959
references.citation: Plant J. 2001 Jun;26(5):509-22
references.pmid: 11439137
references.citation: Plant Physiol. 1999 Dec;121(4):1093-102
references.pmid: 10594097
references.citation: Nat Biotechnol. 2002 Apr;20(4):353-8
references.pmid: 11923840
references.citation: Plant Cell. 2003 Feb;15(2):317-30
references.pmid: 12566575
references.citation: EMBO J. 2001 Oct 1;20(19):5400-11
references.pmid: 11574472
references.citation: Plant Physiol. 2003 Jun;132(2):417-9
references.pmid: 12805572
references.citation: Plant Cell. 2002 Jan;14(1):275-86
references.pmid: 11826312
references.citation: Plant J. 1997 Feb;11(2):301-11
references.pmid: 9076995
references.citation: Mol Plant Microbe Interact. 2000 May;13(5):503-11
references.pmid: 10796016
references.citation: Nature. 2003 Sep 18;425(6955):257-63
references.pmid: 12931144
references.citation: Plant J. 2002 Jan;29(1):11-21
references.pmid: 12060223
references.citation: Plant J. 2003 Jan;33(2):271-83
references.pmid: 12535341
references.citation: Anal Biochem. 1987 Apr;162(1):156-9
references.pmid: 2440339
references.citation: Cell. 2003 Jun 27;113(7):935-44
references.pmid: 12837250
references.citation: Plant Mol Biol. 1995 Dec;29(5):959-68
references.pmid: 8555459
references.citation: Plant Cell. 1992 Mar;4(3):359-366
references.pmid: 12297650
references.citation: Virology. 2000 Feb 15;267(2):318-25
references.pmid: 10662627
references.citation: Science. 1994 Nov 18;266(5188):1247-50
references.pmid: 17810266
references.citation: Plant J. 2001 May;26(4):409-20
references.pmid: 11439128
references.citation: Plant Physiol. 2002 Feb;128(2):552-63
references.pmid: 11842159
references.citation: Plant Cell. 1998 Sep;10(9):1571-80
references.pmid: 9724702
references.citation: Plant Cell. 1998 Jun;10(6):1021-30
references.pmid: 9634589
references.citation: Nucleic Acids Res. 2002 May 15;30(10):e48
references.pmid: 12000853