Determination of gaps by contig alignment with telomere-mediated chromosomal fragmentation in Candida albicans.
We have adopted a method of telomere-mediated chromosome fragmentation in order to demonstrate the alignment of contigs and determination of gaps. We established the order and orientation of four contigs of Candida albicans chromosome 5 and determined the sizes of three gaps between these contigs. We confirmed this proposed alignment of contigs, as well as gap sizes, by sequencing one gap and analyzing three mega deletions of approximately 41 kbp, 58 kbp, and 77 kbp, which covered two other gaps. These gaps could be also conveniently sequenced, which is an important step in establishing a complete sequence. The combined length of contigs and gaps covered approximately 422 kbp, which is one third of chromosome 5. Telomere-mediated chromosome fragmentation, used here for the first time to align the contigs of C. albicans and determine the gaps, proved to be a reliable method. The method could be helpful in sequencing projects of other diploid organisms, in particular those in which centromeres have not been identified. In addition, our approach can be used to assign any contig to a chromosome, or to induce the loss of a specific chromosome.
Kabir
M Anaul
MA
Department of Biochemistry and Biophysics, Box 712, University of Rochester Medical School, Rochester, NY 14642, USA.
Rustchenko
Elena
E
D002176:Candida albicans / Q000235:genetics*; D015825:Chromosomes, Fungal; D020451:Contig Mapping / Q000379:methods*; D053938:DNA Fragmentation; D008969:Molecular Sequence Data; D010957:Plasmids; D016415:Sequence Alignment; D016615:Telomere