title: Screening of LPS-specific peptides from a phage display library using epoxy beads.
abstract: The selection of identical or highly homologous peptides from phage display combinatorial peptide libraries has been unsuccessful in biopanning experiments using microtiter plates. In the present study, by biopanning on LPS-conjugated epoxy beads, we repeatedly enriched clones encoding AWLPWAK and NLQEFLF. These peptides were found to interact with the polysaccharide moiety of LPS, which is highly variable among gram negative bacterial species. In addition, phages encoding these peptides preferentially bound to the LPS of Salmonella family. AWLPWAK-conjugated beads absorbed Salmonella enteritidis from solution and showed a preference for S. enteritidis over Escherichia coli. In summary, this study shows for the first time that a peptide screened from phage displays of combinatorial peptide libraries can be synthesized on beads and be used practically to concentrate bacterial cells from solution.
authors.affiliation: Program in Biochemical Engineering and Biotechnology, Seoul National University, Korea.
authors.forename: Yun-Gon
authors.initials: YG
authors.lastname: Kim
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Chung
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Kim
authors.forename: Dong-Sik
authors.initials: DS
authors.lastname: Shin
authors.forename: Jung-Hyo
authors.initials: JH
authors.lastname: Rhim
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
mesh_terms: D001435:Bacteriophages / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D004853:Epoxy Resins / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D007118:Immunoassay; D008070:Lipopolysaccharides / Q000737:chemistry; D008856:Microscopy, Fluorescence; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D011485:Protein Binding; D012477:Salmonella enteritidis / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D004853:Epoxy Resins; D008070:Lipopolysaccharides; D019151:Peptide Library; D010455:Peptides; D011134:Polysaccharides