title: Myxobacterial biodiversity in an established oak-hickory forest and a savanna restoration site.
abstract: The purpose of this study was to establish the myxobacterial biodiversity of an established oak-hickory forest and a savanna restoration site that has been cut and subsequently burned on four occasions between 1993 and 1998 in an attempt to restore the land to the native savanna ecosystem. Soil and bark samples were processed through standard methods specifically for myxobacteria and numbers and types of species were recorded for both locations. Species were identified through morphology of fruiting bodies, SDS-PAGE of whole cell protein profiles, and 16S rRNA gene sequences. Statistical analyses were employed and suggested that significantly greater numbers and types of myxobacteria are present on the bark of the trees in the established oak-hickory forest than the bark of trees in the savanna restoration site, while little difference in numbers and types of species were observed between the soil samples of the two locations.
authors.affiliation: Science Division, Truman State University, Kirksville, Missouri 63501, USA. ryan-neil@uiowa.edu
authors.forename: R Brock
authors.initials: RB
authors.lastname: Neil
authors.forename: Dan
authors.initials: D
authors.lastname: Hite
authors.forename: Michael I
authors.initials: MI
authors.lastname: Kelrick
authors.forename: Michael L
authors.initials: ML
authors.lastname: Lockhart
authors.forename: Keesoo
authors.initials: K
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D044822:Biodiversity; D031325:Carya / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D005798:Genes, Bacterial; D008923:Missouri; D008969:Molecular Sequence Data; D009231:Myxococcales / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D029963:Quercus / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
references.citation: FEMS Microbiol Rev. 2000 Oct;24(4):403-27
references.pmid: 10978544
references.citation: J Appl Bacteriol. 1969 Mar;32(1):5-12
references.pmid: 5791935
references.citation: Appl Environ Microbiol. 1976 Oct;32(4):642-4
references.pmid: 16345178
references.citation: Can J Microbiol. 1969 Jul;15(7):731-8
references.pmid: 4894284
references.citation: Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9459-63
references.pmid: 1384053
references.citation: Int J Syst Bacteriol. 1999 Jul;49 Pt 3:1255-62
references.pmid: 10425789
references.citation: Science. 1981 Mar 27;211(4489):1437-8
references.pmid: 6162199
references.citation: Microbiol Rev. 1996 Jun;60(2):407-38
references.pmid: 8801440