title: FISH analysis of hematological neoplasias with 1p36 rearrangements allows the definition of a cluster of 2.5 Mb included in the minimal region deleted in 1p36 deletion syndrome.
abstract: Rearrangements in the distal region of the short arm of chromosome 1 are recurrent aberrations in a broad spectrum of human neoplasias. However, neither the location of the breakpoints (BP) on 1p36 nor the candidate genes have been fully determined. We have characterized, by fluorescence in situ hybridization (FISH), the BP in 26 patients with hematological neoplasias and 1p36 rearrangements in the G-banding karyotype. FISH allowed a better characterization of all samples analyzed. Nine cases (35%) showed reciprocal translocations, 15 (58%) unbalanced rearrangements, and two (7%) deletions. We describe two new recurrent aberrations. In 18 of the 26 cases analyzed the BP were located in band 1p36, which is 25.5 Mb long. In 14 of these 18 cases (78%) and without distinction between myeloid and lymphoid neoplasias, the BP clustered in a 2.5 Mb region located between 1p36.32 and the telomere. Interestingly, this region is contained in the 10.5 Mb cluster on 1p36.22-1pter defined in cases with 1p36 deletion syndrome. The 2.5 Mb region, located on 1p36.32-1pter, has a higher frequency of occurrence of tandem repeats and segmental duplications larger than 1 kb, when compared with the 25.5 Mb of the complete 1p36 band. This could explain its proneness for involvement in chromosomal rearrangements in hematological neoplasias.
authors.affiliation: Laboratory of Genetics, Division of Oncology, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII, 55, 31080, Pamplona, Spain. ilahortiga@unav.es
authors.forename: Idoya
authors.initials: I
authors.lastname: Lahortiga
authors.forename: Iria
authors.initials: I
authors.lastname: Vázquez
authors.forename: Elena
authors.initials: E
authors.lastname: Belloni
authors.forename: José P
authors.initials: JP
authors.lastname: Román
authors.forename: Patrizia
authors.initials: P
authors.lastname: Gasparini
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Novo
authors.forename: Isabel
authors.initials: I
authors.lastname: Zudaire
authors.forename: Pier G
authors.initials: PG
authors.lastname: Pelicci
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Hernández
authors.forename: María J
authors.initials: MJ
authors.lastname: Calasanz
authors.forename: María D
authors.initials: MD
authors.lastname: Odero
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D005260:Female; D015321:Gene Rearrangement; D019337:Hematologic Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D013577:Syndrome; D014178:Translocation, Genetic
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