A differential proteome in tumors suppressed by an adenovirus-based skin patch vaccine encoding human carcinoembryonic antigen.
We created an anti-tumor vaccine by using adenovirus as a vector which contains a cytomegalovirus early promoter-directed human carcinoembryonic antigen gene (AdCMV-hCEA). In an attempt to develop the skin patch vaccine, we epicutaneously vaccinated Balb/c mice with AdCMV-hCEA. After nine weeks post-immunization, vaccinated mice evoked a robust antibody titer to CEA and demonstrated the capability of suppressing in vivo growth of implanted murine mammay adenocarioma cell line (JC-hCEA) tumor cells derived from a female Balb/c mouse. Proteomic analysis of the tumor masses in the non-vaccinated naive and vaccinated mice reveal that six proteins change their abundance in the tumor mass. The levels of adenylate kinase 1, beta-enolase, creatine kinase M chain, hemoglobin beta chain and prohibitin were statistically increased whereas the level of a creatine kinase fragment, which is undocumented, was decreased in the tumor of vaccinated mice. These proteins may provide a vital link between early-stage tumor suppression and immune response of skin patch vaccination.
Huang
Chun-Ming
CM
Department of Dermatology, University of Alabama at Birmingham, VH-501, 1670 University Blvd., Birmingham, AL 35294-0019, USA. chunming@uab.edu
Shi
Zhongkai
Z
DeSilva
Tivanka S
TS
Yamamoto
Masato
M
Van Kampen
Kent R
KR
Elmets
Craig A
CA
Tang
De-chu C
DC
D000256:Adenoviridae / Q000235:genetics*; D000279:Administration, Cutaneous; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen / Q000235:genetics*; D045744:Cell Line, Tumor; D003402:Creatine Kinase / Q000378:metabolism; D003587:Cytomegalovirus / Q000235:genetics*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000628:therapy*; D011401:Promoter Regions, Genetic; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000956:Antigens, Viral; D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus; D003402:Creatine Kinase
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