[A study of standardization to the rapid detection of Mycobacterium tuberculosis based on phage amplified biologically assay].
OBJECTIVE
To evaluate the phage amplified biologically (PhaB) assay in the rapid detection of Mycobacteria tuberculosis in samples.


METHODS
The conditions of the PhaB assay, including various infection times prior to addition of virucide and the effect of the inactivation agents which could inactive the extracellular phages, were investigated and compared. The sensitivity, specificity and accuracy of PhaB assay were tested when it was used in rapid detection of Mycobacterium tuberculosis. Some agents of the method, including Mycobacteriophage, virucide and help cells (Mycobacterium smegmatis) were investigated at different times when they were preserved at 4 degrees C.


RESULTS
(1) The optimal infection time prior to addition of virucide was between 3 and 4 hours. Four percent FAS (ferrous ammonium sulphate) could inactive 1 x 10(9) PFU (plaque-forming unit) in five minutes. (2) The samples were positive when 80 - 200 CFU of Mycobacterium tuberculosis were present. However, the positive rate of non-Tuberculosis Mycobateria (NTM) was varied. All bacteria lived in the respiratory tract were negative. (3) The important agents used in this test showed optical effect when they were preserved at 4 degrees C.


CONCLUSIONS
The method based on mycobacteriophage-amplified biologically assay could rapidly detect Mycobacterium tuberculosis, and it was effective, accurate, and simple to perform. It was appropriate for using in developing countries, compared with a variety of molecular techniques.
Peng
Li
L
Department of Pulmonary Diseases, First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China.
Luo
Yong-Ai
YA
Wang
Guo-Zhi
GZ
D000995:Antitubercular Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D009160:Mycobacteriophages; D009164:Mycobacterium Infections / Q000382:microbiology*; D020102:Mycobacterium smegmatis; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification; D013696:Temperature
D000995:Antitubercular Agents; D003470:Culture Media