In-vitro dual binding activity of a evolutionarily related subgroup of hnRNP proteins.
The wide family of heterogeneous nuclear ribonucleoproteins (hnRNPs) comprises members that interact with single-stranded nucleic acids. On the basis of their structure, some of them are characterised by a tandem RNA-binding domain (RBD) and a glycine-rich C-terminus, showing a high degree of homology. Recently, we have isolated some proteins belonging to this group that interact with single-stranded cytosine-block telomeric DNA. The aim of the present investigation is to better characterise the relationship of some structural features shared by these proteins and their in-vitro interaction with the telomeric type sequences. We analysed the in-vitro binding properties of some of these components toward both single-stranded telomeric motifs. Using deletion mutants, the relationship between cytosine-rich motif binding activity and the structural features of one of these proteins is further characterized. This binding activity appears to be related to a subgroup of the 2xRBD+Glycine rich hnRNP, suggesting functionally distinct properties of these proteins, in agreement with their evolutionary relationship.
Bandiera
Antonella
A
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, 34127 Trieste, Italy. bandiera@bbcm.univ.trieste.it
Medic
Nevenka
N
Akindahunsi
A Akintunde
AA
Manzini
Giorgio
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence / Q000235:genetics / Q000502:physiology; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D040681:Structural Homology, Protein; D016615:Telomere / Q000235:genetics / Q000378:metabolism*
D034502:Heterogeneous-Nuclear Ribonucleoprotein D; D022661:RNA, Untranslated
Gene. 1999 Mar 4;228(1-2):13-22
10072754
Genomics. 1998 May 1;49(3):378-84
9615222
J Mol Biol. 2001 Aug 31;311(5):973-88
11531333
Science. 1994 Jul 29;265(5172):615-21
8036511
Curr Opin Cell Biol. 1999 Jun;11(3):363-71
10395553
J Biol Chem. 1998 Jul 24;273(30):18881-90
9668064
Nat Genet. 1998 Jun;19(2):199-202
9620782
J Biol Chem. 1995 Sep 22;270(38):22167-75
7673195
Biochem Biophys Res Commun. 2001 Jan 26;280(3):625-30
11162566
RNA. 1998 Sep;4(9):1111-23
9740129
Eur J Biochem. 2001 Jan;268(1):139-48
11121114
Biochem Biophys Res Commun. 1997 Aug 28;237(3):617-23
9299414
J Mol Biol. 1999 Mar 26;287(2):221-37
10080887
Nucleic Acids Res. 1995 Mar 11;23(5):725-8
7535921
Mol Cell Biol. 1993 Jul;13(7):4301-10
8321232
J Biol Chem. 2000 May 12;275(19):14509-16
10799534
Nucleic Acids Res Suppl. 2003;(3):231-2
14510465
Oncogene. 2002 Jan 21;21(4):580-3
11850782
J Biol Chem. 2003 Jan 24;278(4):2136-40
12435754
Cell. 2001 Sep 21;106(6):661-73
11572773
J Biol Chem. 1997 Feb 14;272(7):4474-82
9020172
Annu Rev Biochem. 1993;62:289-321
8352591
Mol Cell Biol. 2000 Aug;20(15):5425-32
10891483
Genes Dev. 1997 Nov 1;11(21):2801-9
9353250
Genes Dev. 1999 May 1;13(9):1102-15
10323862
Arch Biochem Biophys. 2003 Jan 15;409(2):305-14
12504897
Cell. 1999 May 14;97(4):503-14
10338214
Curr Opin Cell Biol. 2001 Jun;13(3):281-9
11343898