title: Role of central and peripheral mGluR5 receptors in post-operative pain in rats.
abstract: Metabotropic glutamate receptors (mGluRs) have previously been shown to play a role in pain transmission during inflammatory or neuropathic pain states. However, the role of mGluR5 in post-operative pain remains to be fully investigated. The present study was conducted to characterize analgesic activity of 2-methyl-6-(phenylethynyl)-pyridine (MPEP) in the skin-incision-induced post-operative pain model in rats. MPEP is a potent and selective mGluR5 antagonist with high affinity (K(i)=6.3+/-0.9 nM) in rat cortex using [(3)H]-MPEP as a radioligand, while not competing with the mGluR1-selective radioligand [(3)H]-R214127 (K(i)>10,000 nM) in rat cerebellum. Post-operative pain was examined 2 h following surgery using weight-bearing (WB) difference between injured and uninjured paws as a measure of non-evoked pain. In this model, MPEP, as morphine, showed dose-dependent effects and full efficacy after systemic administration (ED(50)=15 mg/kg, i.p. for MPEP, ED(50)=1.3 mg/kg, s.c. for morphine). In addition, intrathecal (i.t.) and intracerebroventricular (i.c.v.) MPEP reduced WB difference (ED(50)=65 microg/rat i.t. and ED(50)=200 microg/rat i.c.v.). Interestingly, intraplantar (i.pl.) injection of MPEP either before or after surgery induced a similar reduction in WB difference (ED(50)=90 microg/rat, i.pl.) while contralateral i.pl. MPEP injection did not produce any effect. These results demonstrate that both peripheral and central mGluR5 receptors play a role in nociceptive transmission observed during post-operative pain. In addition, the data suggest that mGluR5 antagonists could offer a new therapeutic approach to the treatment of post-operative pain.
authors.affiliation: Neuroscience Research, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064-3500, USA. chang.z.zhu@abbott.com
authors.forename: Chang Z
authors.initials: CZ
authors.lastname: Zhu
authors.forename: Gin
authors.initials: G
authors.lastname: Hsieh
authors.forename: Odile
authors.initials: O
authors.lastname: Ei-Kouhen
authors.forename: Sonya G
authors.initials: SG
authors.lastname: Wilson
authors.forename: Joe P
authors.initials: JP
authors.lastname: Mikusa
authors.forename: Peter R
authors.initials: PR
authors.lastname: Hollingsworth
authors.forename: Renjie
authors.initials: R
authors.lastname: Chang
authors.forename: Robert B
authors.initials: RB
authors.lastname: Moreland
authors.forename: Jorge
authors.initials: J
authors.lastname: Brioni
authors.forename: Michael W
authors.initials: MW
authors.lastname: Decker
authors.forename: Prisca
authors.initials: P
authors.lastname: Honore
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D008297:Male; D009020:Morphine / Q000494:pharmacology / Q000627:therapeutic use; D010149:Postoperative Pain / Q000188:drug therapy / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D011725:Pyridines / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: C578937:Grm5 protein, rat; D011725:Pyridines; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; D009020:Morphine; C121465:6-methyl-2-(phenylethynyl)pyridine