title: Effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and central secretory tissues.
abstract: OBJECTIVE
  To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play in acute inflammatory responses.
  
  
  METHODS
  An intestinal ischemia-reperfusion (I/R) injury model of rats was established and rats were divided randomly into six groups: sham-operation group, 60 min ischemia/30 min reperfusion group (I60'R30'), I60'R90', I60'R150', I60'R240' and I60'R360', 9 rats each group. Two highly-sensitive radioimmunoassays for leptin and orexin-A were established and used to check the change of their concentrations in peripheral blood and central secretory tissues before and after intestinal I/R injury.
  
  
  RESULTS
  Compared with the serum leptin level before injury, it decreased significantly in I60'R30' group and increased significantly in I60'R360' group; compared to sham-operation group after injury, serum leptin level increased significantly in I60'R360' group; compared to sham-operation group after injury, adipose leptin levels decreased significantly in I60'R30' and I60'R90' groups, while increased significantly in I60'R360' group. There was no significant difference between the expression levels of orexin-A before and after I/R injury.
  
  
  CONCLUSIONS
  Leptin has a time-dependent response and orexin-A has a delayed response to acute inflammatory stimuli such as intestinal I/R injury and they may participate in metabolic disorders in injury as inflammatory cytokines.
authors.affiliation: Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Lin
authors.forename: Guang-Tao
authors.initials: GT
authors.lastname: Yan
authors.forename: Xiu-Hua
authors.initials: XH
authors.lastname: Hao
authors.forename: Lu-Huan
authors.initials: LH
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D004751:Enteritis / Q000276:immunology / Q000473:pathology; D007031:Hypothalamus / Q000378:metabolism*; D007422:Intestines / Q000473:pathology*; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood* / Q000276:immunology; D020738:Leptin / Q000097:blood* / Q000276:immunology; D008297:Male; D009479:Neuropeptides / Q000097:blood* / Q000276:immunology; D000068797:Orexins; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000276:immunology / Q000473:pathology*
chemical_list: D000906:Antibodies; D047908:Intracellular Signaling Peptides and Proteins; D020738:Leptin; D009479:Neuropeptides; D000068797:Orexins
references.citation: J Immunoassay Immunochem. 2004;25(1):45-55
references.pmid: 15038616
references.citation: Am J Physiol Regul Integr Comp Physiol. 2003 Mar;284(3):R763-70
references.pmid: 12571077
references.citation: J Physiol Biochem. 1999 Mar;55(1):43-9
references.pmid: 10494659
references.citation: J Leukoc Biol. 2000 Oct;68(4):437-46
references.pmid: 11037963
references.citation: Clin Endocrinol (Oxf). 2003 Jun;58(6):770-6
references.pmid: 12780755
references.citation: Biochem Biophys Res Commun. 2002 Aug 9;296(1):129-33
references.pmid: 12147238
references.citation: Eur J Endocrinol. 2001 Jun;144(6):645-51
references.pmid: 11375799
references.citation: Hypertension. 2003 May;41(5):1072-9
references.pmid: 12668587
references.citation: J Interferon Cytokine Res. 2000 Jan;20(1):99-106
references.pmid: 10670656
references.citation: Pancreatology. 2002;2(2):89-99
references.pmid: 12123099
references.citation: Clin Chem. 1998 Mar;44(3):565-70
references.pmid: 9510863
references.citation: Eur J Neurosci. 2004 Mar;19(6):1524-34
references.pmid: 15066149
references.citation: Cell. 1998 Feb 20;92(4):573-85
references.pmid: 9491897
references.citation: J Physiol. 2003 Jun 15;549(Pt 3):809-21
references.pmid: 12702746
references.citation: Diabetes. 2003 Jan;52(1):111-7
references.pmid: 12502500
references.citation: Biochem Pharmacol. 2003 Jul 1;66(1):141-7
references.pmid: 12818374
references.citation: Nutr Rev. 1998 Jun;56(6):172-3
references.pmid: 9656726
references.citation: J Biol Chem. 1998 Dec 25;273(52):35245-9
references.pmid: 9857064
references.citation: J Immunoassay Immunochem. 2002;23(3):317-26
references.pmid: 12227418
references.citation: Gastroenterology. 2002 Jun;122(7):2011-25
references.pmid: 12055606
references.citation: Peptides. 2001 Jan;22(1):139-42
references.pmid: 11179609
references.citation: J Surg Res. 1995 Jun;58(6):558-64
references.pmid: 7791328
references.citation: Cell Immunol. 2000 Jan 10;199(1):15-24
references.pmid: 10675271
references.citation: Brain Res. 2003 Apr 18;969(1-2):95-101
references.pmid: 12676369
references.citation: FASEB J. 1998 Jan;12(1):57-65
references.pmid: 9438411
references.citation: Auton Neurosci. 2002 Jan 10;95(1-2):37-42
references.pmid: 11871784
references.citation: Biochem Biophys Res Commun. 1999 Mar 24;256(3):495-9
references.pmid: 10080926
references.citation: Biochem Biophys Res Commun. 2001 Aug 24;286(3):518-23
references.pmid: 11511089
references.citation: Sheng Li Xue Bao. 2002 Apr 25;54(2):133-8
references.pmid: 11973593
references.citation: Curr Opin Clin Nutr Metab Care. 2003 Jul;6(4):353-60
references.pmid: 12806206
references.citation: World J Gastroenterol. 2003 Jun;9(6):1318-22
references.pmid: 12800248
references.citation: Eur J Biochem. 2002 Feb;269(4):1128-35
references.pmid: 11856342
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Metabolism. 2003 Sep;52(9):1113-6
references.pmid: 14506615
references.citation: Hypertens Res. 2002 Nov;25(6):919-26
references.pmid: 12484517
references.citation: Cell Immunol. 2002 Dec;220(2):143-9
references.pmid: 12657249
references.citation: Ann N Y Acad Sci. 2002 Oct;971:522-7
references.pmid: 12438173
references.citation: Am J Pathol. 2000 May;156(5):1781-7
references.pmid: 10793089
references.citation: J Clin Endocrinol Metab. 2002 Jul;87(7):3040-6
references.pmid: 12107198
references.citation: Diabetes. 2002 Aug;51(8):2434-40
references.pmid: 12145155