[Effects of Tianzhi Keli on extracellular acetylcholine and catecholamine levels in striatum of rats with neuromitochondrial impairment].
OBJECTIVE
To investigate the effects of Tianzhi Keli (TZ) on acetylcholine (ACh) and catecholamine levels in striatum of rats with neuromitochondrial impairment, and try to find out the neuroprotective mechanism of TZ.


METHODS
The microdialysis and high performance liquid chromatography (HPLC)-post column Immobilized enzyme reactor (IMER)-electrochemical detection (ED) were used to establish a model of mitochondrial energy metabolism impairment which induced by perfusion with sodium azide (NaN3), and measure continuously the effects of TZ on extracellular ACh, choline (Ch) and catecholamine of model rats.


RESULTS
After perfusion with NaN3, ACh, noradrenalin (NE), adrenaline (E), dopamine (DA), 3,4-Dihydroxyphenyl-aletic (DOPAC), and homovanillic acid (HVA) levels were decreased obviously (P < 0.05-0.01), while Ch level was increased distinctly (P < 0.01). Transmitters levels were recovered individually after stop the perfusion with NaN3. TZ can postpone the decrease of ACh and advance the recover of Ch. The effect of TZ coupled with duxil on increasing ACh level is more obviously than effect of TZ or duxil. TZ is also showing a tendency to postpone the decrease of catecholamine and advance its recovery. TZ coupled with duxil can advance the recovery of DOPAC and adjust the metabolic abnormity positively.


CONCLUSIONS
TZ has effect on protecting impairment of choline neurosystem, which induced by damage of mitochondrion and abnormity of energy metabolism; coupled with duxil have synergistic action. TZ also has tendency to protect the impairment of epinephrine and dopamine neurosystem.
Sun
Xiao-Fang
XF
Geriatrics Institute, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China.
Wang
Wei
W
Wang
Dan-Qiao
DQ
Zhao
De-Zhong
DZ
Du
Gui-you
GY
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005110:Extracellular Space / Q000378:metabolism; D031668:Gastrodia / Q000737:chemistry; D008297:Male; D017551:Microdialysis; D028361:Mitochondrial Diseases / Q000139:chemically induced / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D019810:Sodium Azide; D032070:Uncaria / Q000737:chemistry
D002395:Catecholamines; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D015102:3,4-Dihydroxyphenylacetic Acid; D019810:Sodium Azide; D000109:Acetylcholine; D004298:Dopamine; D009638:Norepinephrine