title: Molecular epidemiology of HIV-1 subtypes in southern China.
abstract: The driving forces of the HIV-1 epidemic in Guangxi, China were assessed by investigating virologic and epidemiologic data from a cohort of longitudinally followed injection drug users (IDUs) in Binyang and Pingxiang, major urban areas along 2 separate drug routes in the province. Sera and interview data were obtained in September and October of 2000. Sequence analysis of HIV-1 was performed on the gag-pol region (HXB2 nt 1850-3005) and C2 to V4 env (HXB2 nt 6704-7626). Sequence data demonstrated that 88% of the infections in Pingxiang were CRF01_AE, whereas 96% in Bin-yang were CRF08_BC. Three recently infected subjects in Pingxiang were infected with CRF08_BC, and 1 chronically infected subject had evidence of a recombinant virus. Intersubject distances were statistically greater for CRF01_AE-infected subjects than CRF08_BC-infected subjects for all regions except V4. The epidemic in Binyang is similar to previously described IDU-based epidemics, with a strong founder effect with little variation in V3. The epidemic in Pingxiang may have had multiple introductions of the CRF01_AE epidemic into the city and greater spread through sexual transmission, resulting in greater variation in V3 than typically seen in purely parenterally based epidemics.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Oliver
authors.initials: O
authors.lastname: Laeyendecker
authors.forename: Guang Wen
authors.initials: GW
authors.lastname: Zhang
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Quinn
authors.forename: Rebecca
authors.initials: R
authors.lastname: Garten
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Ray
authors.forename: Shenghan
authors.initials: S
authors.lastname: Lai
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Yu
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D018076:DNA, Complementary; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D015685:Fusion Proteins, gag-pol / Q000235:genetics; D015686:Gene Products, env / Q000737:chemistry; D015752:Genes, env; D015750:Genes, gag; D015751:Genes, pol; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D016333:HIV Protease / Q000737:chemistry / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000737:chemistry / Q000235:genetics; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015819:Substance Abuse, Intravenous / Q000150:complications; D014505:Urban Population
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D015685:Fusion Proteins, gag-pol; D015686:Gene Products, env; D054303:HIV Reverse Transcriptase; D016333:HIV Protease