title: Perfluoroarylazide derivatives of 2'-O-modified oligoribonucleotides: efficient reagents for RNA photomodification.
abstract: Site-specific photomodification of the 5'-terminal fragment of MDR1 mRNA by perfluoroarylazide derivatives of 2'-O-modified (2'-O-methyl or 2'-O-tetrahydropyranyl) oligoribo- and oligodeoxyribonucleotides was investigated. The conjugates built of 2'-O-modified oligoribonucleotides demonstrate beneficial features compared with their deoxyribo analogs: the extent of RNA modification by 2'-O-modified oligoribonucleotides and oligodeoxyribonucleotide conjugates was 40- 50% and 20%, respectively.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Novopashina
authors.forename: R N
authors.initials: RN
authors.lastname: Kulikov
authors.forename: M A
authors.initials: MA
authors.lastname: Kuznetsova
authors.forename: A G
authors.initials: AG
authors.lastname: Venyaminova
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: V V
authors.initials: VV
authors.lastname: Vlassov
mesh_terms: D001386:Azides / Q000737:chemistry*; D020029:Base Pairing; D019450:Genes, MDR / Q000235:genetics*; D009843:Oligoribonucleotides / Q000737:chemistry* / Q000235:genetics; D010777:Photochemistry; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D001386:Azides; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012260:Ribonucleases