Anti-endothelial cell antibodies (AECA) in patients with propylthiouracil (PTU)-induced ANCA positive vasculitis are associated with disease activity.
Increasing evidence has demonstrated that propylthiouracil (PTU) could induce ANCA positive vasculitis. However, our previous work has suggested that only one-fifth of the PTU-induced ANCA positive patients had clinical vasculitis and so the mechanism is not clear. Anti-endothelial cell antibodies (AECA) have been implicated in the pathogenesis of various vasculitides, including primary ANCA positive systemic vasculitis. The purpose of this study is to investigate the prevalence of AECA and their possible role in the pathogenesis of patients with PTU-induced ANCA positive vasculitis. Sera from 11 patients with PTU-induced ANCA positive vasculitis at both active and quiescent phases, and sera from 10 patients with PTU-induced ANCA but without clinical vasculitis, were studied. Sera from 30 healthy blood donors were collected as normal controls. Soluble proteins from 1% Triton-100 extracted in vitro cultured human umbilical vein endothelial cells were used as antigens and an immunoblotting technique was performed to determine the presence of AECA, and their specific target antigens were identified. In patients with PTU-induced ANCA positive vasculitis, 10 of the 11 patients in an active phase of disease were serum IgG-AECA positive and six protein bands of endothelial antigens could be blotted (61 kD, 69 kD, 77 kD, 85 kD, 91 kD and 97 kD). However, in the quiescent phase, seven of the 10 positive sera turned negative. None of the ANCA positive but vasculitis negative patients or normal controls were AECA positive. In conclusion, AECA could be found in sera from patients with PTU-induced ANCA positive vasculitis and were associated more closely with vasculitic disease activity.
Yu
F
F
Institutes Renal Division and Institute of Nephrology, Peking University First Hospital, Beijing, China.
Zhao
M-H
MH
Zhang
Y-K
YK
Zhang
Y
Y
Wang
H-Y
HY
D000293:Adolescent; D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000276:immunology*; D013956:Antithyroid Agents / Q000009:adverse effects* / Q000627:therapeutic use; D015153:Blotting, Western / Q000379:methods; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D016009:Chi-Square Distribution; D042783:Endothelial Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011441:Propylthiouracil / Q000009:adverse effects* / Q000627:therapeutic use; D014657:Vasculitis / Q000139:chemically induced* / Q000276:immunology
D019268:Antibodies, Antineutrophil Cytoplasmic; D013956:Antithyroid Agents; D011441:Propylthiouracil
Kidney Int. 2004 May;65(5):1564-7
15086893
Clin Exp Immunol. 2002 Sep;129(3):562-70
12197900
Curr Rheumatol Rep. 2004 Jun;6(3):184-94
15134597
Clin Exp Immunol. 2000 Jan;119(1):203-9
10606984
Clin Exp Immunol. 2003 Mar;131(3):528-35
12605707
Zhonghua Yi Xue Za Zhi. 2003 Jun 10;83(11):932-5
12899790
Lancet. 1993 Sep 11;342(8872):651-2
8103148
Transplantation. 1988 Apr;45(4):787-92
3282360
Clin Immunol Immunopathol. 1992 Jun;63(3):267-74
1623646
Arthritis Rheum. 2003 Jul;48(7):2025-35
12847697
Clin Exp Immunol. 2003 Nov;134(2):360-4
14616799
Clin Exp Immunol. 2000 May;120(2):392-8
10792393
Clin Exp Immunol. 1997 Jan;107(1):120-6
9010266
Am J Kidney Dis. 1996 Aug;28(2):186-94
8768912
Respirology. 2003 Dec;8(4):532-5
14708556
Clin Endocrinol (Oxf). 2001 May;54(5):651-4
11380496
J Clin Endocrinol Metab. 2000 Nov;85(11):4270-3
11095466
Clin Exp Rheumatol. 2002 Jan-Feb;20(1):19-26
11892703
Clin Exp Immunol. 1996 Dec;106(3):523-8
8973622
Zhonghua Nei Ke Za Zhi. 2002 Jun;41(6):404-7
12137604
Clin Exp Immunol. 1991 Jul;85(1):14-9
2070557
Clin Exp Immunol. 2002 Nov;130(2):233-40
12390310
Clin Exp Immunol. 1999 Mar;115(3):567-70
10193435
Clin Exp Immunol. 2004 Jan;135(1):164-72
14678279
Arthritis Rheum. 1996 May;39(5):758-66
8639172
Clin Exp Immunol. 2003 Jun;132(3):505-8
12780699
Clin Exp Immunol. 2002 Jul;129(1):54-60
12100022
Rev Rhum Engl Ed. 1995 Dec;62(11):737-47
8869215
Clin Exp Immunol. 2002 Jul;129(1):183-90
12100039
Clin Exp Immunol. 2003 Apr;132(1):174-9
12653854
Endocr J. 2002 Jun;49(3):329-34
12201217
J Am Soc Nephrol. 2004 Mar;15(3):796-808
14978183
Neth J Med. 2003 Sep;61(9):296-9
14692444
Clin Immunol Immunopathol. 1994 Mar;70(3):211-6
8313658
Clin Exp Rheumatol. 2003 Nov-Dec;21(6 Suppl 32):S35-48
14740426
Clin Exp Immunol. 2002 Nov;130(2):352-7
12390327
Clin Exp Immunol. 1998 Mar;111(3):491-6
9528888
Kidney Int. 2004 Jun;65(6):2172-83
15149330
Clin Exp Immunol. 2004 Oct;138(1):179-82
15373922
Hum Immunol. 2004 Jan;65(1):1-12
14700590
Arthritis Rheum. 1999 Jul;42(7):1421-32
10403270
Arthritis Rheum. 2000 Feb;43(2):405-13
10693882
Clin Exp Immunol. 2001 Dec;126(3):584-8
11737080
Clin Exp Immunol. 2001 Jun;124(3):492-501
11472414