[Molecular mechanism of cleft palate induced by retinoic acid].
OBJECTIVE
To explore the molecular mechanism of cleft palate induced by chemicals.


METHODS
Retinoic acid was used as a known teratogen to induce cleft palate in ICR mice and a suppression subtractive hybridization (SSH) technique was applied to identify differentially expressed genes that related to cleft palate of ICR mice.


RESULTS
14 reverse differently and 9 forward differentially expressed clones were obtained. Some clones were selected to be sequenced and aligned to GenBank.


CONCLUSIONS
In this study, suppressed Gpc3 and Insulin-Induced protein 1 could affect growth of palate shelves and resulted in cleft palate by reducing the size of the palate shelves. Down-regulation of Ptprs interfered with a cell signal pathway and down-regulation of Tn C inhibited the cell de-adhesion and expression of Egfr, then suppressed Egfr prevented the normal expression of MMPs that influenced the medial edge epithelium disruption and caused cleft palate. Tn C could bind to Ptprs and Gpcs, and HSPGs were ligands for Ptrps. Up-regulate of Rps25 might play a role in cleft palate by excessively apoptosis.
Zhu
Jiangbo
J
Department of Toxicology, Second Military Medical University, Shanghai 200433, China.
Yin
Muquan
M
Zhu
Yuping
Y
Chen
Rongfang
R
Wan
Xuying
X
Ma
Xili
X
Guo
Miaoli
M
Zhang
Tianbao
T
D000818:Animals; D017209:Apoptosis; D002972:Cleft Palate / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053673:Glypicans; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D009693:Nucleic Acid Hybridization; D019063:Tenascin / Q000235:genetics; D014212:Tretinoin / Q000633:toxicity*; D015854:Up-Regulation
D053673:Glypicans; D019812:Heparan Sulfate Proteoglycans; C506119:Insig1 protein, mouse; D008565:Membrane Proteins; D019063:Tenascin; D014212:Tretinoin