title: [Pharmacokinetics of oxymatrine and its metabolite in beagle dogs by LC-MS].
abstract: OBJECTIVE
  To establish LC-MS method in the determination of oxymatrine and its metabolite in plasma and investigate their pharmacokinetics in beagle dogs.
  
  
  METHODS
  Lichrospher C18 column (4.6 mm x 250 mm, 5 microm) was used as the analytical column maintained at 25 degrees C. The mobile phase consisted of 10 mmol x L(-1) CH3COONH4 and CH3OH (25:75). Flow rate was 1 mL x min(-1). Electrospray ionization (ESI) was carried out. The ESI ion source was set in positive ion polasity mode. The selective ion monitoring (SIM) was set at m/z 265.1 and 249.2.
  
  
  RESULTS
  The linearity ranged from 2 to 5000 ng x mL(-1) (r = 0.9991). The detection of oxymatrine and its metabolite were 0.6 and 0.3 ng x mL(-1). The RSD(%) within day and between day was less than 4.7%. The recovery of this method was more than 96.5%. The disposition was conformed to a two-compartment model. The T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of oxymatrine were (5.5+/-1.58) h, (1.0+/-0.30) h, (2418.3 +/-970.78) ng x mL(-1), (3.2+/-0.64) h, (5797.4+/-908.16) ng x mL(-1) x h accordingly. The corresponding T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of matrine were (9.8+/-2.77) h, (1.9+/-1.09) h, (1532.4+/-494.86) ng x mL(-1), (4.4+/-1.97) h, (5530.5+/-1042.65) ng x mL(-1) x h.
  
  
  CONCLUSIONS
  This assay was highly sensitive, rapid, simple and specific enough for determining concentrations of oxymatrine and its metabolite matrine in plasma of beagle dog.
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210038, China.
authors.forename: Su-jun
authors.initials: SJ
authors.lastname: Wang
authors.forename: Guang-ji
authors.initials: GJ
authors.lastname: Wang
authors.forename: Xiao-tian
authors.initials: XT
authors.lastname: Li
authors.forename: Ren-ling
authors.initials: RL
authors.lastname: Ma
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Sun
authors.forename: Long-sheng
authors.initials: LS
authors.lastname: Sheng
mesh_terms: D000284:Administration, Oral; D000470:Alkaloids / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid; D004285:Dogs; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011807:Quinolizines; D029909:Sophora / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines