A fluorescent assay for chloride transport; identification of a synthetic anionophore with improved activity.
A fluorescent assay based on the chloride-sensitive probe, lucigenin, is developed for monitoring chloride transport into vesicles, and used to compare the effectiveness of three steroid-derived transporters.
McNally
Beth A
BA
Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA.
Koulov
Atanas V
AV
Smith
Bradley D
BD
Joos
Jean-Baptiste
JB
Davis
Anthony P
AP
D000166:Acridines / Q000737:chemistry*; D002712:Chlorides / Q000032:analysis* / Q000378:metabolism*; D005453:Fluorescence; D017136:Ion Transport; D049408:Luminescent Agents / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence / Q000379:methods
D000166:Acridines; D002712:Chlorides; D049408:Luminescent Agents; C033472:10,10'-dimethyl-9,9'-biacridinium