Computational analysis of 3'-ends of ESTs shows four classes of alternative polyadenylation in human, mouse, and rat.
Alternative initiation, splicing, and polyadenylation are key mechanisms used by many organisms to generate diversity among mature mRNA transcripts originating from the same transcription unit. While previous computational analyses of alternative polyadenylation have focused on polyadenylation activities within or downstream of the normal 3'-terminal exons, we present the results of the first genome-wide analysis of patterns of alternative polyadenylation in the human, mouse, and rat genomes occurring over the entire transcribed regions of mRNAs using 3'-ESTs with poly(A) tails aligned to genomic sequences. Four distinct classes of patterns of alternative polyadenylation result from this analysis: tandem poly(A) sites, composite exons, hidden exons, and truncated exons. We estimate that at least 49% (human), 31% (mouse), and 28% (rat) of polyadenylated transcription units have alternative polyadenylation. A portion of these alternative polyadenylation events result in new protein isoforms.
Yan
Jun
J
Institute of Arctic Biology, University of Alaska, Fairbanks, Alaska 99775-7000, USA. fsjy1@uaf.edu
Marr
Thomas G
TG
D017398:Alternative Splicing; D000818:Animals; D020224:Expressed Sequence Tags; D006801:Humans; D034904:Lamin Type A / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D034904:Lamin Type A; D012333:RNA, Messenger; C440308:lamin C
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