title: Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates.
abstract: Cell-cycle events are controlled by cyclin-dependent kinases (CDKs), whose periodic activation is driven by cyclins. Different cyclins promote distinct cell-cycle events, but the molecular basis for these differences remains unclear. Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. About 24% of these proteins were phosphorylated more efficiently by Clb5-Cdk1 than Clb2-Cdk1. The Clb5-specific targets include several proteins (Sld2, Cdc6, Orc6, Mcm3 and Cdh1) involved in early S-phase events. Clb5 specificity depended on an interaction between a hydrophobic patch in Clb5 and a short sequence in the substrate (the RXL or Cy motif). Phosphorylation of Clb5-specific targets during S phase was reduced by replacing Clb5 with Clb2 or by mutating the substrate RXL motif, confirming the importance of Clb5 specificity in vivo. Although we did not identify any highly Clb2-specific substrates, we found that Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets. Thus, Clb5 and Clb2 use distinct mechanisms to enhance the phosphorylation of S-phase and M-phase substrates.
authors.affiliation: Department of Physiology, University of California, San Francisco, California 94143-2200, USA.
authors.forename: Mart
authors.initials: M
authors.lastname: Loog
authors.forename: David O
authors.initials: DO
authors.lastname: Morgan
mesh_terms: D020816:Amino Acid Motifs; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism*; D002455:Cell Division; D019926:Cyclin B / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism; D016193:G1 Phase; D057927:Hydrophobic and Hydrophilic Interactions; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: C093749:CLB2 protein, S cerevisiae; C076558:CLB5 protein, S cerevisiae; D019926:Cyclin B; D003598:Cytoskeletal Proteins; C456282:FIN1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C102333:histone H1 kinase; D017489:CDC28 Protein Kinase, S cerevisiae