Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis.
A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed.
Zamfir
Alina D
AD
Institute for Medical Physics and Biophysics, Biomedical Analysis, University of Munster, Germany. zamfir@uni-muenster.de
Lion
Niels
N
Vukelic
Zeljka
Z
Bindila
Laura
L
Rossier
Joel
J
Girault
Hubert H
HH
Peter-Katalinic
Jasna
J
D000596:Amino Acids / Q000032:analysis / Q000652:urine; D001923:Brain Chemistry; D002555:Cerebrospinal Fluid / Q000737:chemistry; D005732:Gangliosides / Q000032:analysis; D006001:Glycoconjugates / Q000032:analysis* / Q000652:urine; D006020:Glycopeptides / Q000032:analysis / Q000652:urine; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D046208:Microchip Analytical Procedures / Q000379:methods; D008875:Middle Aged; D009330:Nebulizers and Vaporizers; D012015:Reference Standards; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
D000596:Amino Acids; D005732:Gangliosides; D006001:Glycoconjugates; D006020:Glycopeptides