Modulation of tumor induced angiogenesis in Ehrlich ascites tumor.
In this study the enzyme glutaminase, purified from the ascites fluid of ovarian cancer patients, was analysed for its antiangiogenic activity. Intraperitoneal administration of this enzyme reduces the number of tumor directed capillaries in solid and ascites tumor bearing Swiss mice induced by transplantation of Ehrlich ascites cells. The enzyme has a critical role in regulating the secretion of vascular endothelial growth factor (VEGF) from tumor cell and in turn tumor growth. Glutamine analogue like 6-diazo, 5- oxo L-norleucine (DON) is also found to be effective in regulating vascular endothelial growth factor (VEGF) secretion from tumor cells in vitro. Treatment with enzyme reduced serum VEGF levels of the tumor induced animals. In vitro VEGF production by EAC cells was reduced in a concentration dependent manner in presence of glutamine analogue.
Ghosh
S
S
Dept. of Metabolic Regulation, Chittaranjan National Cancer Institute, Kolkata, India.
Roy
S
S
Banerjee
M
M
Maity
P
P
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001835:Body Weight; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003980:Diazooxonorleucine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005972:Glutaminase / Q000378:metabolism; D005973:Glutamine / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000473:pathology; D009389:Neovascularization, Pathologic; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism
D000970:Antineoplastic Agents; D042461:Vascular Endothelial Growth Factor A; D003980:Diazooxonorleucine; D005973:Glutamine; D005972:Glutaminase