title: The first crystal structure of a macromolecular assembly under high pressure: CpMV at 330 MPa.
abstract: The structure of cubic Cowpea mosaic virus crystals, compressed at 330 MPa in a diamond anvil cell, was refined at 2.8 A from data collected using ultrashort-wavelength (0.331 A) synchrotron radiation. With respect to the structure at atmospheric pressure, order is increased with lower Debye Waller factors and a larger number of ordered water molecules. Hydrogen-bond lengths are on average shorter and the cavity volume is strongly reduced. A tentative mechanistic explanation is given for the coexistence of disordered and ordered cubic crystals in crystallization drops and for the disorder-order transition observed in disordered crystals submitted to high pressure. Based on such explanation, it can be concluded that pressure would in general improve, albeit to a variable extent, the order in macromolecular crystals.
authors.affiliation: Synchrotron SOLEIL, Gif sur Yvette, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Girard
authors.forename: Richard
authors.initials: R
authors.lastname: Kahn
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mezouar
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Dhaussy
authors.forename: Tianwei
authors.initials: T
authors.lastname: Lin
authors.forename: John E
authors.initials: JE
authors.lastname: Johnson
authors.forename: Roger
authors.initials: R
authors.lastname: Fourme
mesh_terms: D001703:Biophysics / Q000379:methods*; D002213:Capsid / Q000737:chemistry*; D017798:Comovirus / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D011312:Pressure; D011506:Proteins / Q000737:chemistry; D012984:Software; D013499:Surface Properties; D017356:Synchrotrons; D013696:Temperature; D014867:Water
chemical_list: D046911:Macromolecular Substances; D011506:Proteins; D014867:Water
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