Separation of denatured proteins in free solution on a microchip based on differential binding of alkyl sulfates with different carbon chain lengths.
Denatured proteins were separated in free solution on a microfluidic chip without any sieving matrix after carrying out the denaturation with a mixture of alkyl sulfates with different carbon chain lengths.
Lu
Chang
C
Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA. changlu@purdue.edu
Smith
Aaron E
AE
Craighead
Harold G
HG
D002244:Carbon / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods*; D011489:Protein Denaturation / Q000187:drug effects; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012996:Solutions
D011506:Proteins; D012996:Solutions; D002244:Carbon