Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein.
A novel methoxypolyethylene glycol (mPEG) derivative, containing a reactive group of 1-methyl pyridinium toluene-4-sulfonate, was synthesized and characterized. The mPEG derivative was successfully conjugated with two proteins: recombinant human granulocyte-colony stimulating factor (rhG-CSF) and consensus interferon (C-IFN). Homogeneous mono-PEGylated proteins were obtained which were identified by high performance size-exclusion chromatography and MALDI-TOF mass spectrometry. The biological activities of the mono-PEGylated rhG-CSF and the mono-PEGylated C-IFN were maintained at 90% and 88%, respectively.
Yun
Qiang
Q
National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.
Chen
Ting
T
Zhang
Guifeng
G
Bi
Jingxiu
J
Ma
Guanghui
G
Su
Zhiguo
Z
D002850:Chromatography, Gel; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007370:Interferon Type I / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry*; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007370:Interferon Type I; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol