Natural recombinant between equine herpesviruses 1 and 4 in the ICP4 gene.
Equine herpesvirus 1 (EHV-1) is a pathogen causing rhinopneumonia in young horses, abortion in mares, and myeloencephalitis in adult horses. Two types, EHV-1 P and EHV-1 B, have recently been dominant among 16 electropherotypes. EHV-1 P and EHV-1 B viruses were compared by long and accurate polymerase chain reaction (LA-PCR) and restriction fragment length polymorphism (RFLP) analysis. Differences in restriction sites were found to be focused in ORF64, which encodes the infected cell protein 4 (ICP4), and downstream of the ICP4 gene. The 3 ' -end and downstream of ICP4 gene of EHV-1 B were found to be replaced by the corresponding region of EHV-4, indicating that EHV-1 B is a naturally occurring recombinant virus between progenitors of EHV-1 P and EHV-4. This is the first report showing a natural interspecies recombinant in alphaherpesviruses.
Pagamjav
Ochir
O
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Japan.
Sakata
Tohru
T
Matsumura
Tomio
T
Yamaguchi
Tsuyoshi
T
Fukushi
Hideto
H
D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D006566:Herpesviridae Infections / Q000473:pathology / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000302:isolation & purification; D023141:Herpesvirus 4, Equid / Q000235:genetics* / Q000302:isolation & purification; D006736:Horses; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins