title: Effect of platelet-rich-plasma on cranial distraction osteogenesis in sheep: preliminary clinical and radiographic results.
abstract: The purpose of this study was to investigate the effect of platelet-rich-plasma (PRP) on cranial distraction osteogenesis. Standardized calvaria critical size defects (6 cm x 5 cm) were created in 16 adult female sheep. Bifocal cranial transport distraction osteogenesis with autogenous free calvaria bone grafts (2 cm x 4 cm) was performed at a rate of 1mm once daily to a total of 30 mm. The 16 sheep involved in the experiment were randomly divided into four groups, four animals in each: Group 1 (no PRP, latency 5 days); Group 2 (no PRP, latency 0 day), Group 3 (PRP, latency 5 days) and Group 4 (PRP, latency 0 day). After a consolidation phase of 6 weeks, the animals were sacrificed and specimens harvested for conventional radiological and 3D quantitative computer tomographic (3D-QCT) assessment. New bone was generated in the distraction zone in all groups. There were significantly (P < 0.05) higher densities in the proximal region of the distraction regenerate in Group 4 (PRP, latency 0 day) compared to Group 2. However, no significant differences in mean density of the total distraction regenerate were found, neither in volume of the bony regenerate between the experimental groups. This study showed that PRP only had an effect on bone regeneration if active distraction was started immediately after application of PRP in the distraction gap.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. gwen.swennen@gmx.de
authors.forename: G R J
authors.initials: GR
authors.lastname: Swennen
authors.forename: F
authors.initials: F
authors.lastname: Schutyser
authors.forename: M C
authors.initials: MC
authors.lastname: Mueller
authors.forename: F-J
authors.initials: FJ
authors.lastname: Kramer
authors.forename: C
authors.initials: C
authors.lastname: Eulzer
authors.forename: H
authors.initials: H
authors.lastname: Schliephake
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001792:Blood Platelets; D001861:Bone Regeneration / Q000187:drug effects*; D016025:Bone Transplantation; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D021621:Imaging, Three-Dimensional; D023421:Models, Animal; D019857:Osteogenesis, Distraction; D010956:Plasmapheresis; D011897:Random Allocation; D012756:Sheep; D034561:Sheep, Domestic; D012886:Skull / Q000601:surgery*; D013997:Time Factors; D014057:Tomography, X-Ray Computed
chemical_list: D006133:Growth Substances