title: A carbohydrate fraction, AIP1, from Artemisia iwayomogi down-regulates Fas gene expression and suppresses apoptotic death of the thymocytes induced by 2,3,7,8-tectrachlorodibenzo-p-dioxin.
abstract: Apoptotic death of mouse thymocytes in vitro, as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), involves the up-regulation of Fas gene expression, while a carbohydrate fraction, AIP1, from Artemisia iwayomogi suppresses the death of thymocytes in culture along with the down-regulation of Fas gene expression. We have now investigated whether the AIP1 fraction modulates TCDD-induced thymocyte death. When treated with TCDD and AIP1 fraction together, the thymocytes do not show apoptosis induced by the TCDD treatment. The AIP1 supplementation to the TCDD treatment also down-regulates the TCDD-induced Fas gene up-regulation. These findings indicate that the AIP1 fraction suppresses TCDD-induced thymocyte apoptosis through the modulation of Fas gene expression.
authors.affiliation: Department of Microbiology, School of Biotechnology & Biomedical Science and Bio-Health Product Research Center, Inje University, Gimhae, Gyungnam, 621-749, S. Korea.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Ji
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Yeo
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Lee
authors.forename: Jung Suk
authors.initials: JS
authors.lastname: Hwang
authors.forename: Kyung Ah
authors.initials: KA
authors.lastname: Koo
authors.forename: Seon Woo
authors.initials: SW
authors.lastname: Cheong
authors.forename: Joo Hung
authors.initials: JH
authors.lastname: Park
authors.forename: Gap Soo
authors.initials: GS
authors.lastname: Oh
authors.forename: Chun Sik
authors.initials: CS
authors.lastname: Yoon
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Youn
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D019306:Artemisia / Q000378:metabolism*; D002241:Carbohydrates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004147:Dioxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004347:Drug Interactions; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism*; D019014:fas Receptor
chemical_list: D002241:Carbohydrates; D004147:Dioxins; C491899:Fas protein, mouse; D018124:Receptors, Tumor Necrosis Factor; D019014:fas Receptor; C066841:2,3,7,8-tetrabromodibenzo-4-dioxin