Experimental prediction of the evolution of ceftazidime resistance in the CTX-M-2 extended-spectrum beta-lactamase.
We applied in vitro evolution to an Escherichia coli strain containing bla(CTX-M-2) and obtained 10 independent mutant bla(CTX-M-2) alleles that confer elevated resistance to ceftazidime (MIC > or = 32 microg/ml) but lost the ability to confer resistance to cefepime. All alleles had a Pro-to-Ser substitution at position 167.
Welsh
Kerry J
KJ
Department of Epidemiology, Rollins School of Public Health, Emory University, 1518 Clifton Rd., Atlanta, GA 30322, USA.
Barlow
Miriam
M
Tenover
Fred C
FC
Biddle
James W
JW
Rasheed
J Kamile
JK
Clark
Leigh Ann
LA
McGowan
John E
JE
D000483:Alleles; D002442:Ceftazidime / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D008826:Microbial Sensitivity Tests; D009154:Mutation; D001618:beta-Lactamases / Q000235:genetics*
D002442:Ceftazidime; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases
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