title: Protein modifications in the plant secretory pathway: current status and practical implications in molecular pharming.
abstract: Plants have become, over the last ten years, a suitable alternative to microbial and animal cell factories for the production of clinically-useful, therapeutic proteins. Besides the well known advantage of low-cost and large-scale production of safe and biologically active mammalian proteins, plants also are able to perform most post-translational maturations required for biological activity and suitable pharmacokinetics of recombinant therapeutic proteins. In this short review we focus on glycosylation and proteolytic processing of plant-made pharmaceuticals during their transport through the plant cell's secretory pathway. We also address the practical implications of these important processes on the effectiveness of plant molecular pharming systems.
authors.affiliation: CNRS UMR 6037, IFRMP 23, GDR 2590, Université de Rouen-Bâtiment de biologie (extension), Blvd de Broglie, 76821 Mont Saint Aignan Cedex, France. lfaye@crihan.fr
authors.forename: Loïc
authors.initials: L
authors.lastname: Faye
authors.forename: Aurelia
authors.initials: A
authors.lastname: Boulaflous
authors.forename: Meriem
authors.initials: M
authors.lastname: Benchabane
authors.forename: Véronique
authors.initials: V
authors.lastname: Gomord
authors.forename: Dominique
authors.initials: D
authors.lastname: Michaud
mesh_terms: D000818:Animals; D006031:Glycosylation; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D010447:Peptide Hydrolases