Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells.
We tested antiproliferative activity of selected cruciferous phytoalexins including brassinin, 1-methoxybrassinin, (+/-)-spirobrassin, (+/-)-1-methoxyspirobrassinin and (+/-)-1-methoxyspirobrassinol, in leukemic Jurkat cell. The most effective of the tested phytoalexins was 1-methoxybrassinin with IC(50) 10 micromol l(-1). However, significant effect of all phytoalexines was also determined at concentration 1 micromol l(-1). In 1-methoxybrassinin-treated Jurkat cells, we found significant increase in the fraction of cells with a sub-G(0)/G(1) DNA content, which is considered to be a marker of cell death by apoptosis. Apoptosis was also confirmed by the annexin V staining. In summary, 1-methoxybrassinin exerted potent antiproliferative activity probably due to cell cycle arrest and apoptosis induction.
Pilátová
M
M
Department of Pharmacology, Faculty of Medicine, P.J. Safárik University, Kosice, Slovakia.
Sarisský
M
M
Kutschy
P
P
Mirossay
A
A
Mezencev
R
R
Curillová
Z
Z
Suchý
M
M
Monde
K
K
Mirossay
L
L
Mojzis
J
J
D017209:Apoptosis / Q000187:drug effects*; D019607:Brassicaceae; D016923:Cell Death / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000633:toxicity*; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins
D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins