title: Sulforaphane, erucin, and iberin up-regulate thioredoxin reductase 1 expression in human MCF-7 cells.
abstract: Isothiocyanates (ITCs) found in cruciferous vegetables are potentially important anticarcinogenic phytochemicals for many types of cancers including breast cancer. In this study, we have shown that three isothiocyanates, sulforaphane, erucin, and iberin, are potent inducers of thioredoxin reductase 1 (TrxR1) in human breast cancer MCF-7 cells. Sulforaphane, erucin, and iberin at 1 microM induce TrxR1 mRNA 2-3-fold within 8 h of treatment, and induce mRNA 5-7-fold with 12 microM ITC treatments. Selenium did not affect sulforaphane-induced TrxR1 mRNA levels, but significantly enhanced both TrxR1 protein expression (up to 9-fold in erucin treatment) and corresponding activities. These results suggest that dietary ITCs are important factors in the regulation of redox status through the induction of the selenoprotein thioredoxin reductase.
authors.affiliation: Nutrition Division, Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, UK.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Shuran
authors.initials: S
authors.lastname: Wang
authors.forename: A Forbes
authors.initials: AF
authors.lastname: Howie
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Beckett
authors.forename: Richard
authors.initials: R
authors.lastname: Mithen
authors.forename: Yongping
authors.initials: Y
authors.lastname: Bao
mesh_terms: D001943:Breast Neoplasms; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012643:Selenium / Q000494:pharmacology*; D013440:Sulfides / Q000494:pharmacology*; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology*; D054481:Thioredoxin Reductase 1; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D017879:Isothiocyanates; D012333:RNA, Messenger; D013440:Sulfides; D013454:Sulfoxides; D013861:Thiocyanates; C082585:iberin; C073539:erucin; C516124:TXNRD1 protein, human; D054481:Thioredoxin Reductase 1; D013880:Thioredoxin-Disulfide Reductase; C016766:sulforaphane; D012643:Selenium