Influence of amphotericin B deoxycholate or amphotericin B colloidal dispersion on renal tubule epithelium in rat.
Amphotericin B deoxycholate (AmB) or Amphotericin B colloidal dispersion (ABCD) are used in clinics for the treatment of systemic fungal infections. The goal of our study was to compare the nephrotoxicity of these drugs in rat kidney. The effects of AmB and ABCD on the ultrastructure of the epithelium of renal tubules were studied and evaluated using morphometric and statistical methods. Two groups of 3 animals were established: group 1 was treated with AmB desoxycholate and group 2, to which ABCD was applied. AmB caused more than ABCD ultrastructural changes in the cytoplasm of the epithelial cells: damage to mitochondria, vacuolation of cytoplasm, and increased values of volume density of peroxisomes. However, we failed to observe significant differences in morphology and density of the other cell organelles. The proximal tubules seemed to be more sensitive to the nephrotoxic influence of both formulas than the distal tubules of rat kidney. Although, AmB causes more severe damage than ABCD, both drugs cause damage to renal tubuli.
Krejcírová
Lenka
L
Department of Histology and Embryology, Medicine Faculty Masaryk University Brno, Czech Republic. lkrejcir@med.muni.cz
Lauschová
Irena
I
Horký
Drahomír
D
Doubek
Michal
M
Mayer
Jirí
J
Doubek
Jirí
J
D000666:Amphotericin B / Q000633:toxicity*; D000818:Animals; D000935:Antifungal Agents / Q000633:toxicity*; D003840:Deoxycholic Acid / Q000633:toxicity*; D004338:Drug Combinations; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D007684:Kidney Tubules / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051381:Rats
D000935:Antifungal Agents; D004338:Drug Combinations; D003840:Deoxycholic Acid; D000666:Amphotericin B; C059765:amphotericin B, deoxycholate drug combination