Cultivation of endothelial cells on adhesive protein-free synthetic polymer gels.
Various hydrogels without modification by any cell adhesive proteins have been investigated as cell scaffolds. The present study shows that bovine fetal aorta endothelial cells can adhere, spread, proliferate, and reach confluence on poly(acrylic acid), poly(sodium p-styrene sulfonate), and poly(2-acrylamido-2- methyl-1-propanesulfonic sodium) gels, whereas cells reach subconfluence on poly(vinyl alcohol) and poly(methacrylic acid) gels. The proliferation behavior was sensitive to both hydrogel charge density and crosslinker concentration. The relationship between cell proliferation and zeta potential of gels was discussed. It was found that hydrogels with a negative zeta potential higher than about 20 mV facilitates cell proliferation.
Chen
Yong Mei
YM
Graduate School of Science, Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo 060-0810, Japan.
Shiraishi
Nozomi
N
Satokawa
Hideo
H
Kakugo
Akira
A
Narita
Tetsuharu
T
Gong
Jian Ping
JP
Osada
Yoshihito
Y
Yamamoto
Kimiko
K
Ando
Joji
J
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D011108:Polymers
D011108:Polymers