Mycobacterium tuberculosis H37Rv3377c encodes the diterpene cyclase for producing the halimane skeleton.
The cloning and functional expression of Mycobacterium tuberculosis Rv3377c in Escherichia coli revealed that this gene encodes the diterpene cyclase for producing (+)-5(6),13-halimadiene-15-ol, which accepts geranylgeranyldiphosphate as the intrinsic substrate.
Nakano
Chiaki
C
Department of Applied Biological Chemistry, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, Ikarashi, Niigata, 950-2181, Japan.
Okamura
Tomoo
T
Sato
Tsutomu
T
Dairi
Tohru
T
Hoshino
Tsutomu
T
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D003500:Cyclization; D004224:Diterpenes / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics*
C518124:5(6),13-halimadiene-15-ol; D001426:Bacterial Proteins; D004224:Diterpenes