title: Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses.
abstract: A pepper gene, CABPR1, which encodes basic pathogenesis-related protein 1, has been reported to be strongly induced after ethephon treatment, wounding, and tobacco mosaic virus infection. The potential role of CABPR1 in tolerance of biotic or abiotic stresses was examined in transgenic Nicotiana tabacum cv. xanthi plants. Overexpression of CABPR1 in tobacco plants enhanced tolerance not only to heavy metal stresses, but also to the oomycete pathogen Phytophthora nicotianae, and the bacterial pathogens Ralstonia solanacearum and Pseudomonas syringae pv. tabaci. RT-PCR revealed that the CABPR1 transgene increased expression of the PR-Q and glutathione S-transferase genes, but decreased expression of the PR-1a and thaumatin genes. Moreover, these transgenic lines exhibited significant decreases in total peroxidase activity and transcription level, suggesting that overexpression of CABPR1 in tobacco cells altered the balance of redox systems. Redox imbalance in transgenic lines may lead to H(2)O(2) accumulation, triggering tolerance to biotic and abiotic stresses.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. ssarowar@hotmail.com
authors.forename: Sujon
authors.initials: S
authors.lastname: Sarowar
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Eui Nam
authors.initials: EN
authors.lastname: Kim
authors.forename: Ki Deok
authors.initials: KD
authors.lastname: Kim
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Hwang
authors.forename: Rafiul
authors.initials: R
authors.lastname: Islam
authors.forename: Jeong Sheop
authors.initials: JS
authors.lastname: Shin
mesh_terms: D002212:Capsicum / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics*; D019216:Metals, Heavy / Q000633:toxicity; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics; D010544:Peroxidases / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D019076:Transgenes / Q000235:genetics
chemical_list: D019216:Metals, Heavy; D010940:Plant Proteins; C003427:thaumatin protein, plant; D006861:Hydrogen Peroxide; D010544:Peroxidases; D005982:Glutathione Transferase
references.citation: Mol Cells. 1999 Aug 31;9(4):376-83
references.pmid: 10515600
references.citation: Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7327-31
references.pmid: 8346252
references.citation: Biotechnol Lett. 2004 Jan;26(2):153-7
references.pmid: 15000484
references.citation: Virology. 1989 Nov;173(1):89-97
references.pmid: 2815592
references.citation: Transgenic Res. 2003 Jun;12(3):363-7
references.pmid: 12779124
references.citation: Plant Physiol. 1995 Jul;108(3):919-27
references.pmid: 7630972
references.citation: J Exp Bot. 2002 May;53(372):1305-19
references.pmid: 11997377
references.citation: Plant Sci. 2000 Sep 8;158(1-2):19-32
references.pmid: 10996241
references.citation: Plant Mol Biol. 1988 Mar;11(2):89-94
references.pmid: 24272252
references.citation: Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3182-7
references.pmid: 8622910
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:127-158
references.pmid: 15012285
references.citation: Plant J. 1997 May;11(5):993-1005
references.pmid: 9193071
references.citation: Proc Natl Acad Sci U S A. 1990 Jan;87(1):98-102
references.pmid: 2296608
references.citation: Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):56-9
references.pmid: 1729717
references.citation: Cell. 1994 Nov 18;79(4):583-93
references.pmid: 7954825
references.citation: Plant Physiol. 1991 Jun;96(2):390-7
references.pmid: 16668198
references.citation: J Biol Chem. 1997 Aug 22;272(34):20998-1001
references.pmid: 9261100
references.citation: Plant Physiol. 1995 May;108(1):17-27
references.pmid: 7784503
references.citation: Nature. 1986 May 29-Jun 4;321(6069):531-2
references.pmid: 3713832
references.citation: J Lab Clin Med. 1954 Aug;44(2):301-7
references.pmid: 13184240
references.citation: Mol Plant Microbe Interact. 2003 Mar;16(3):196-205
references.pmid: 12650451
references.citation: Plant J. 2000 Jul;23(2):205-13
references.pmid: 10929114
references.citation: Science. 1997 Jun 6;276(5318):1566-8
references.pmid: 9171061
references.citation: Plant Physiol. 1993 Mar;101(3):857-863
references.pmid: 12231736
references.citation: Plant Physiol. 1989 Jul;90(3):1096-101
references.pmid: 16666857
references.citation: Plant Physiol. 1998 Jul;117(3):1103-14
references.pmid: 9662553
references.citation: Nucleic Acids Res. 1991 Mar 25;19(6):1349
references.pmid: 2030957
references.citation: Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1888-92
references.pmid: 11607463