Diethyldithiocarbamate inhibits iNOS expression in human lens epithelial cells stimulated by IFN-gamma and LPS.
OBJECTIVE
To investigate the biological activity of human lens epithelial cells (HLEC) in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and to assesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels and expression of NOS.


METHODS
The human lens epithelial cell line SRA 01/04 was used in this experiment. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect, respectively, iNOS mRNA expression and protein production.


RESULTS
A costimulation by interferon gamma (IFN-gamma) and lipopolysaccharide (LPS) was necessary for iNOS expression in HLEC. The expression of iNOS was significantly reduced in a dose-dependent manner by adding DDC from 10 micromol/L to 1 mmol/L.


CONCLUSIONS
The expression of iNOS in HLEC needs co-stimulation with IFN-gamma and LPS and it is inhibited by DDC.
Li
De-xin
DX
Department of Pharmaceutics, College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wang
Si-ling
SL
Ito
Yoshimasa
Y
Zhang
Jian-hai
JH
Wu
Chun-fu
CF
D002460:Cell Line; D004050:Ditiocarb / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000201:enzymology; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology / Q000201:enzymology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D004050:Ditiocarb; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II