title: Pyruvate kinase regulatory element 1 (PKR-RE1) mediates hexokinase gene expression in K562 cells.
abstract: We have established the functional importance of PKR-RE1, a necessary transcriptional regulatory element in the erythroid-specific promoter of the human pyruvate kinase gene (PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA-protein interaction at PKR-RE1 involves a CTGTC motif. Because the same motif is also present in the erythroid-specific promoter of the hexokinase gene (HK1), we confirmed its functional relevance by in vitro transfection in K562 cells. Moreover, EMSA demonstrated that the CTGTC motif in both the PKLR and HK1 promoters mediates binding of the same protein. Therefore, we postulate a more general role of PKR-RE1 in erythroid-specific gene expression.
authors.affiliation: Department of Clinical Chemistry, University Medical Center Utrecht, PO Box 85500, NL-3508 GA, Utrecht, The Netherlands.
authors.forename: Karen M K
authors.initials: KM
authors.lastname: de Vooght
authors.forename: Richard
authors.initials: R
authors.lastname: van Wijk
authors.forename: Brigitte A
authors.initials: BA
authors.lastname: van Oirschot
authors.forename: Gert
authors.initials: G
authors.lastname: Rijksen
authors.forename: Wouter W
authors.initials: WW
authors.lastname: van Solinge
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015972:Gene Expression Regulation, Neoplastic; D006593:Hexokinase / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D011401:Promoter Regions, Genetic; D038621:Regulatory Sequences, Ribonucleic Acid; D014162:Transfection
chemical_list: D017931:DNA Primers; D038621:Regulatory Sequences, Ribonucleic Acid; D006593:Hexokinase