title: Characterization of Haemonchus contortus calreticulin suggests its role in feeding and immune evasion by the parasite.
abstract: Haemonchus contortus, a gastrointestinal parasite of sheep and goat feeds on the blood of its host and causes bleeding at the biting site. In this report, we demonstrate that the Ca2+ binding protein, calreticulin (CalR), is present in excretory/secretory products of adult worms. The secreted CalR enhanced plasma coagulation time. Using recombinant fragments, this property has been mapped to C-terminal part of the molecule which has binding sites for Ca2+ as well as clotting factors. Complement protein C1q bound to immobilized CalR and C1q dependent lysis of sensitized sheep erythrocytes was inhibited by CalR, a function mapped to N-domain of the protein. Factor X and a 24 kDa polypeptide derived from prothrombin but not prothrombin bound to immobilized CalR. The binding site for 24 kDa polypeptide in the CalR molecule has been localized in the P-domain. Our results suggest at least two functions for secreted CalR: first, to prevent blood clotting by binding to Ca2+ and clotting factors thus enabling parasite to feed on the host blood and second to modulate the host immune response by binding to complement C1q thereby facilitating parasite's survival within the host.
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar (UP) 243 122, India.
authors.forename: Sajja
authors.initials: S
authors.lastname: Suchitra
authors.forename: Paritosh
authors.initials: P
authors.lastname: Joshi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D037282:Calreticulin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats / Q000469:parasitology; D006189:Haemonchus / Q000276:immunology / Q000502:physiology*; D008969:Molecular Sequence Data; D012756:Sheep / Q000469:parasitology
chemical_list: D037282:Calreticulin; D017931:DNA Primers