title: Novel polyomavirus detected in the feces of a chimpanzee by nested broad-spectrum PCR.
abstract: In order to screen for new polyomaviruses in samples derived from various animal species, degenerated PCR primer pairs were constructed. By using a nested PCR protocol, the sensitive detection of nine different polyomavirus genomes was demonstrated. The screening of field samples revealed the presence of a new polyomavirus, tentatively designated chimpanzee polyomavirus (ChPyV), in the feces of a juvenile chimpanzee (Pan troglodytes). Analysis of the region encoding the major capsid protein VP1 revealed a unique insertion in the EF loop of the protein and showed that ChPyV is a distinct virus related to the monkey polyomavirus B-lymphotropic polyomavirus and the human polyomavirus JC polyomavirus.
authors.affiliation: Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany. johne@vetmed.uni-leipzig.de
authors.forename: Reimar
authors.initials: R
authors.lastname: Johne
authors.forename: Dirk
authors.initials: D
authors.lastname: Enderlein
authors.forename: Hermann
authors.initials: H
authors.lastname: Nieper
authors.forename: Hermann
authors.initials: H
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018420:Ape Diseases / Q000821:virology*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000821:virology*; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000145:classification / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D017386:Sequence Homology, Amino Acid; D014412:Tumor Virus Infections / Q000662:veterinary / Q000821:virology
chemical_list: D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus
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