Doxorubicin hepatotoxicity and hepatic free radical metabolism in rats. The effects of vitamin E and catechin.
Doxorubicin (DXR) is an anthracycline antibiotic, broadly used in tumor therapy. In the present study we investigated whether vitamin E and catechin can reduce the toxic effects of doxorubicin. Vitamin E (200 IU/kg/week), catechin (200 mg/kg/week), doxorubicin (5 mg/kg/week), doxorubicin+vitamin E (200 IU/kg/week), doxorubicin+catechin (200 mg/kg/week) combinations were given to rats weighing 210-230 g (n=6/group). Changes in major enzymes participating in free radical metabolism superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GSHPx), catalase (CAT) and malondialdehyde (MDA) were evaluated in the livers of all animals. Superoxide dismutase and catalase activity increased in the doxorubicin-treated group compared to control (P<0.05). Glutathione peroxidase levels increased in the catechin+doxorubicin-treated group (P<0.05) and reached maximum concentrations in the doxorubicin-treated group compared to control (P<0.01). Malondialdehyde levels increased in the doxorubicin-treated group compared to control and all-treated groups (P<0.05). Malondialdehyde, glutathione peroxidase and catalase activities were decreased in the vitamin E+doxorubicin- and catechin+doxorubicin-treated group compared to doxorubicin-treated group (P<0.05). All enzymes activities showed no statistical differences in the not mentioned groups above (P>0.05). Electron microscopic studies supported biochemical findings. We conclude that vitamin E and catechin significantly reduce doxorubicin-induced hepatotoxicity in rats.
Kalender
Yusuf
Y
Department of Biology, Faculty of Arts and Science, Gazi University, 06500 Teknikokullar, Ankara, Turkey. kalender@gazi.edu.tr
Yel
Mustafa
M
Kalender
Suna
S
D000818:Animals; D002392:Catechin / Q000494:pharmacology* / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury; D004317:Doxorubicin / Q000633:toxicity*; D005609:Free Radicals / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008107:Liver Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology* / Q000627:therapeutic use
D005609:Free Radicals; D014810:Vitamin E; D004317:Doxorubicin; D002392:Catechin