title: Screening of inhibitors for influenza A virus using high-performance affinity chromatography and combinatorial peptide libraries.
abstract: The affinity inhibitor of fusion peptide of influenza A virus has been studied using a combination of high-performance affinity chromatography (HPAC) and combinatorial peptide libraries. Fusion peptide (FP) (1-11) of influenza A virus was used as the affinity ligand and immobilized onto the poly(glycidyl methacrylate) (PGMA) beads. Positional scanning peptide libraries based on antisense peptide strategy and extended peptide libraries were designed and synthesized. The screening was carried out at acidic pH (5.5) in order to imitate the environment of virus fusion. A hendecapeptide FHRKKGRGKHK was identified to have a strong affinity to the FP (1-11). The dissociation constant of the complex of the hendecapeptide and the FP (1-11) is 3.10 x 10(-6) mol l(-1) in a physiological buffer condition. The polypeptide has a fairly inhibitory effect on three different strains of influenza A virus H1N1 subtype.
authors.affiliation: Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Zhong Guan Cun, Beijing 100080, China. zhaorui@iccas.ac.cn
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Canliang
authors.initials: C
authors.lastname: Fanga
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.forename: Dihua
authors.initials: D
authors.lastname: Shangguan
authors.forename: Shaoxiang
authors.initials: S
authors.lastname: Xiong
authors.forename: Tiansheng
authors.initials: T
authors.lastname: Su
authors.forename: Guoquan
authors.initials: G
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D009980:Influenza A virus / Q000187:drug effects*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D009842:Oligopeptides