Agglutination study of poly(allyl-alpha-D-glucopyranose/styrene) latex particles in the presence of Concanavalin A.
Latex agglutination of the glucose-modified latex, which were synthesized by emulsion copolymerization of allyl-modified glucose and styrene monomers by specific binding interactions between Concanavalin A (Con A) and allyl-alpha-d-glucopyranose, were investigated. The surface of the glucose-modified latex was characterized by dye-partition method and the number of glucose was 1517 per latex particle. The average particle size and the polydispersity index of the latex were 78.3 and 1.005, respectively. Time-evolution adsorption behavior of various concentrations of the Con A and the consequent latex agglutination were studied by UV spectrophotometer at 540 nm and zeta-potential analyzer at the fixed latex concentration of 0.02 wt%. Specific binding between Con A and allyl-alpha-D-glucopyranose leaded the latex particles to coagulate by decreasing the electrostatic repulsion between the particles and mobility.
Lee
Jung Yul
JY
Nanosphere Process and Technology Laboratory, Department of Chemical Engineering, Yonsei University, 134 Shinchon-Dong, Seodaemoon-Ku, Seoul 120-749, South Korea.
Cheong
In Woo
IW
Kim
Jung Hyun
JH
D000371:Agglutination; D003208:Concanavalin A / Q000737:chemistry*; D004355:Drug Stability; D005947:Glucose; D005960:Glucosides / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008968:Molecular Conformation; D011137:Polystyrenes / Q000737:chemistry*
D005960:Glucosides; D011137:Polystyrenes; C503400:poly(allyl glucopyranose-styrene); D003208:Concanavalin A; D005947:Glucose
Annu Rev Biochem. 1996;65:441-73
8811186
Appl Microbiol Biotechnol. 1993 Nov;40(2-3):365-9
7764393
Enzyme Microb Technol. 2000 Sep 1;27(6):356-361
10938414
Chem Rev. 1998 Apr 2;98(2):833-862
11848916
J Colloid Interface Sci. 1997 Feb 1;186(1):71-9
9056304
Chem Rev. 1998 Apr 2;98(2):637-674
11848911
J Biomed Mater Res. 1993 Jul;27(7):837-50
8360211
Can J Biochem. 1968 Sep;46(9):1147-50
4972079
Glycobiology. 1993 Apr;3(2):97-130
8490246
Chem Rev. 1996 Mar 28;96(2):683-720
11848770
Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4620-4
15070767
Biotechnol Bioeng. 1989 Apr 5;33(9):1205-9
18588039
Biomaterials. 1998 Jun;19(11-12):987-98
9692797
Appl Microbiol Biotechnol. 1994 Mar;41(1):99-105
7764637