Oxidative state in platelets and erythrocytes in aging and Alzheimer's disease.
Several studies have shown involvement of peroxynitrite anion, a potent oxidative agent, in Alzheimer's disease (AD) neuropathology. Herein, we assessed in platelets and erythrocytes of AD patients, age-matched and young adults controls: thiobarbituric acid-reactive substances (TBARS) production; superoxide dismutase (SOD), nitric oxide synthase (NOS) and Na,K-ATPase activities; cyclic GMP (cGMP) content, both basal and after sodium nitroprusside (SNP) stimulation. Aging was associated with an increase in TBARS production and NOS activity, a decrease in basal cGMP content and no change in SOD and Na,K-ATPase activities. AD patients, compared to aged controls, have: increase in TBARS production and in NOS, SOD and Na,K-ATPase activities but no alteration in basal cGMP content. SNP increased cGMP platelets production in all groups. In conclusion, we demonstrated in platelets and erythrocytes a disruption in systemic modulation of oxidative stress in aging and with more intensity in AD.
Kawamoto
Elisa Mitiko
EM
Department of Pharmacology, School of Medicine, University of São Paulo, São Paulo, Brazil.
Munhoz
Carolina Demarchi
CD
Glezer
Isaias
I
Bahia
Valéria Santoro
VS
Caramelli
Paulo
P
Nitrini
Ricardo
R
Gorjão
Renata
R
Curi
Rui
R
Scavone
Cristoforo
C
Marcourakis
Tania
T
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000544:Alzheimer Disease / Q000097:blood* / Q000473:pathology; D001792:Blood Platelets / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010100:Oxygen / Q000097:blood*; D017382:Reactive Oxygen Species / Q000097:blood*
D017382:Reactive Oxygen Species; D010100:Oxygen