The origin of extrinsic nitrergic axons supplying the human eye.
Nitrergic nerve fibres of intrinsic and extrinsic origin constitute an important component of the autonomic innervation in the human eye. The intrinsic source of nitrergic nerves are the ganglion cells in choroid and ciliary muscle. In order to obtain more information on the origin of extrinsic nitrergic nerves in the human eye, we obtained superior cervical, ciliary, pterygopalatine and trigeminal ganglia from six human donors, and stained them for neuronal nitric oxide synthase (nNOS) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-D). In the superior cervical ganglia, nNOS/NADPH-D-positive varicose axons were observed whereas perikarya were consistently negative. Fewer than 1% of perikarya in the ciliary ganglia were labelled for nNOS/NADPH-D. The diameter of nNOS/NADPH-D-positive ciliary perikarya was between 8 and 10 microm, which was markedly smaller than the diameter of the vast majority of negative perikarya in the ciliary ganglion. More than 70% of perikarya in the pterygopalatine ganglia were intensely labelled for both nNOS and NADPH-D. In trigeminal ganglia, 18% of perikarya were nNOS/NADPH-D-positive. The average diameter of trigeminal nNOS/NADPH-D perikarya was between 25 and 45 microm. Pterygopalatine and trigeminal ganglia are the most likely sources for extrinsic nerve fibres to the human eye.
Gottanka
Johannes
J
Department of Anatomy, University of Erlangen-Nürnberg, Erlangen, Germany.
Kirch
Wolfram
W
Tamm
Ernst R
ER
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001369:Axons / Q000502:physiology*; D002924:Ciliary Body / Q000737:chemistry / Q000502:physiology; D005123:Eye / Q000294:innervation*; D006801:Humans; D008875:Middle Aged; D009252:NADPH Dehydrogenase / Q000032:analysis; D009434:Neural Pathways; D026602:Nitrergic Neurons / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000032:analysis; D013194:Staining and Labeling; D017783:Superior Cervical Ganglion / Q000737:chemistry / Q000502:physiology
D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase
Brain Res Bull. 1995;36(5):491-4
7536106
Brain Res. 1999 May 1;826(2):193-9
10224296
Neuroreport. 1993 Jan;4(1):62-4
8453037
Invest Ophthalmol Vis Sci. 2002 Mar;43(3):581-6
11867570
Invest Ophthalmol Vis Sci. 1994 Dec;35(13):4268-72
7528181
J Histochem Cytochem. 1985 Jul;33(7):711-4
2409130
Ophthalmologica. 1996;210(5):296-302
8878213
Invest Ophthalmol Vis Sci. 1995 Jul;36(8):1633-44
7601644
Ophthalmologica. 1997;211(3):201-4
9176902
Br J Pharmacol. 1997 Apr;120(8):1491-6
9113370
Acta Otolaryngol. 1997 Sep;117(5):735-7
9349872
Invest Ophthalmol Vis Sci. 1995 May;36(6):1125-36
7730022
J Biol Chem. 1992 Jun 5;267(16):11374-8
1375940
Klin Monbl Augenheilkd. 1993 Jul;203(1):77-82
8411894
Invest Ophthalmol Vis Sci. 1994 Feb;35(2):592-9
7509326
Invest Ophthalmol Vis Sci. 2003 Sep;44(9):3705-12
12939283
Invest Ophthalmol Vis Sci. 1995 Feb;36(2):414-26
7531186
Nature. 1967 Oct 14;216(5111):173-4
4862079
Neurosci Lett. 1999 Mar 26;263(2-3):121-4
10213150
Br J Pharmacol. 1996 Jul;118(6):1537-43
8832083
J Auton Nerv Syst. 1999 May 28;76(2-3):176-83
10412842
Invest Ophthalmol Vis Sci. 2004 Feb;45(2):361-7
14744873