title: Protective effect of fibronectin for endotoxin-induced liver injury after partial hepatectomy in rats.
abstract: BACKGROUND
  Endotoxemia may occur after hepatectomy and become the cause of post-operative death. Fibronectins (Fns) are involved in a number of biological processes, such as cellular adhesion, motility, differentiation, apoptosis, hemostasis, wound healing, and ischemic injury. Studies were performed to determine whether Fn influences the survival rate of rats subjected to endotoxin-induced liver injury after partial hepatectomy.
  
  
  METHODS
  Lipopolysaccharide (LPS) was administered intravenously to male Sprague-Dawley rats within 48 h of 70% hepatectomy. Before LPS administration, plasma Fn or bovine serum albumin was given intravenously.
  
  
  RESULTS
  The survival rate of the Fn-treated group was markedly improved compared with that of the controls. Fn prevented increases in the concentrations of serum enzymes and total bilirubin related to liver injury. The levels of inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1beta, and cytokine-induced neutrophil chemoattractant, in serum and liver tissue, also were significantly lower in the Fn-treated group than in the control group. Furthermore, the degree of apoptosis and necrosis in remnant liver was significantly decreased in the Fn-treated rats compared with the controls.
  
  
  CONCLUSIONS
  These results indicate that Fn prevents endotoxin-induced liver injury after partial hepatectomy, at least in part through the inhibition of production of inflammatory cytokines, necrosis and apoptosis in the remaining liver.
authors.affiliation: Department of Surgery, KansaiMedical University, Moriguchi, Osaka, Japan.
authors.forename: Takamichi
authors.initials: T
authors.lastname: Saito
authors.forename: A-H O N
authors.initials: AH
authors.lastname: Kwon
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Qiu
authors.forename: Hideto
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Katsushige
authors.initials: K
authors.lastname: Tsuji
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kamiyama
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D016207:Cytokines / Q000032:analysis; D019446:Endotoxemia / Q000382:microbiology; D004731:Endotoxins / Q000009:adverse effects; D016326:Extracellular Matrix Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D005353:Fibronectins / Q000494:pharmacology / Q000627:therapeutic use*; D006498:Hepatectomy / Q000009:adverse effects*; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D008107:Liver Diseases / Q000382:microbiology / Q000517:prevention & control*; D008297:Male; D023421:Models, Animal; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016019:Survival Analysis
chemical_list: D016207:Cytokines; D004731:Endotoxins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D008070:Lipopolysaccharides