title: Disruption of lateral olivocochlear neurons via a dopaminergic neurotoxin depresses sound-evoked auditory nerve activity.
abstract: We applied the dopaminergic (DA) neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to the guinea pig cochlear perilymph. Immunolabeling of lateral olivocochlear (LOC) neurons using antibodies against synaptophysin was reduced after the MPTP treatment. In contrast, labeling of the medial olivocochlear innervation remained intact. As after brainstem lesions of the lateral superior olive (LSO), the site of origin of the LOC neurons, the main effect of disrupting LOC innervation of the cochlea via MPTP was a depression of the amplitude of the compound action potential (CAP). CAP amplitude depression was similar to that produced by LSO lesions. Latency of the N1 component of the CAP, and distortion product otoacoustic emission amplitude and adaptation were unchanged by the MPTP treatment. This technique for selectively lesioning descending LOC efferents provides a new opportunity for examining LOC modulation of afferent activity and behavioral measures of perception.
authors.affiliation: Kresge Hearing Research Institute, University of Michigan Medical School, Ann Arbor, MI 48109-0506, USA. colleeng@umich.edu
authors.forename: Colleen G
authors.initials: CG
authors.lastname: Le Prell
authors.forename: Kärin
authors.initials: K
authors.lastname: Halsey
authors.forename: Larry F
authors.initials: LF
authors.lastname: Hughes
authors.forename: David F
authors.initials: DF
authors.lastname: Dolan
authors.forename: Sanford C
authors.initials: SC
authors.lastname: Bledsoe
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology; D000200:Action Potentials; D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology; D000818:Animals; D003056:Cochlear Nerve / Q000502:physiology*; D017626:Cochlear Nucleus / Q000473:pathology / Q000502:physiology*; D003714:Denervation; D004298:Dopamine / Q000502:physiology*; D015259:Dopamine Agents / Q000494:pharmacology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009498:Neurotoxins / Q000494:pharmacology; D009847:Olivary Nucleus / Q000473:pathology / Q000502:physiology*; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects / Q000502:physiology
chemical_list: D015259:Dopamine Agents; D009498:Neurotoxins; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D004298:Dopamine
references.citation: Neurochem Res. 1998 Aug;23(8):1133-8
references.pmid: 9704604
references.citation: Neuroscience. 1999 Apr;90(1):131-8
references.pmid: 10188940
references.citation: J Neurophysiol. 1995 Aug;74(2):598-615
references.pmid: 7472367
references.citation: Brain Res. 1987 Sep 1;419(1-2):347-51
references.pmid: 2890408
references.citation: Prog Neurobiol. 1997 Oct;53(3):331-80
references.pmid: 9364616
references.citation: J Neurochem. 1993 Oct;61(4):1191-206
references.pmid: 8376979
references.citation: Neuroreport. 2001 Mar 26;12(4):803-7
references.pmid: 11277587
references.citation: Acta Otolaryngol. 1993 May;113(3):330-4
references.pmid: 8100108
references.citation: J Pharmacol Exp Ther. 1969 Feb;165(2):300-9
references.pmid: 5763065
references.citation: Hear Res. 1996 May;95(1-2):161-7
references.pmid: 8793517
references.citation: Hear Res. 2005 Mar;201(1-2):99-108
references.pmid: 15721565
references.citation: J Neurosci. 2000 Jul 1;20(13):5163-9
references.pmid: 10864973
references.citation: J Neurocytol. 1999 Mar;28(3):197-206
references.pmid: 10617902
references.citation: Hear Res. 2000 Oct;148(1-2):124-36
references.pmid: 10978830
references.citation: J Comp Neurol. 1996 Aug 05;371(4):621-32
references.pmid: 8841914
references.citation: Hear Res. 1990 Nov;49(1-3):209-23
references.pmid: 1705540
references.citation: J Assoc Res Otolaryngol. 2000 Aug;1(1):82-8
references.pmid: 11548239
references.citation: Hear Res. 1995 Oct;90(1-2):202-11
references.pmid: 8974998
references.citation: J Comp Neurol. 1999 Mar 29;406(1):72-86
references.pmid: 10100893
references.citation: Neuroscience. 1993 May;54(1):133-42
references.pmid: 8100046
references.citation: Hear Res. 1998 Oct;124(1-2):17-26
references.pmid: 9822899
references.citation: Prog Neurobiol. 1995 Dec;47(6):449-76
references.pmid: 8787031
references.citation: Physiol Rev. 1993 Apr;73(2):309-73
references.pmid: 8097330
references.citation: J Histochem Cytochem. 1984 Aug;32(8):839-43
references.pmid: 6379037
references.citation: Neuroscience. 2004;125(3):725-33
references.pmid: 15099686
references.citation: J Acoust Soc Am. 1970 Oct;48(4):966-77
references.pmid: 5480391
references.citation: Eur J Neurosci. 2001 Sep;14(6):977-86
references.pmid: 11595036
references.citation: Hear Res. 1985 Mar;17(3):249-58
references.pmid: 2862132
references.citation: J Neurosci. 1998 May 15;18(10):3859-69
references.pmid: 9570815
references.citation: J Neurophysiol. 1991 May;65(5):1158-69
references.pmid: 1651373
references.citation: Neuroreport. 1996 Mar 22;7(4):889-94
references.pmid: 8724667
references.citation: Am J Otolaryngol. 1986 Mar-Apr;7(2):100-6
references.pmid: 2870655
references.citation: Hear Res. 1994 Apr;74(1-2):122-34
references.pmid: 8040083
references.citation: Hear Res. 1999 Feb;128(1-2):75-9
references.pmid: 10082286
references.citation: Life Sci. 2000 Jun 8;67(3):241-50
references.pmid: 10983868
references.citation: Brain Res. 1991 Mar 22;544(1):94-100
references.pmid: 1855140
references.citation: Brain Res. 1984 May 28;301(1):187-91
references.pmid: 6610458
references.citation: J Neurophysiol. 1956 Sep;19(5):424-37
references.pmid: 13367873
references.citation: J Comp Neurol. 1987 Jun 22;260(4):605-18
references.pmid: 3611413
references.citation: Acta Otolaryngol. 1993 Jul;113(4):512-8
references.pmid: 8379307
references.citation: J Neurophysiol. 1988 Aug;60(2):549-68
references.pmid: 3171641
references.citation: Toxicol Lett. 2003 Apr 4;139(2-3):135-51
references.pmid: 12628749
references.citation: Neurotoxicol Teratol. 1999 Mar-Apr;21(2):169-75
references.pmid: 10192277
references.citation: Ann N Y Acad Sci. 1999 Nov 28;884:305-11
references.pmid: 10842602
references.citation: Hear Res. 1987;30(1):33-8
references.pmid: 2890616
references.citation: J Histochem Cytochem. 1988 Jul;36(7):797-801
references.pmid: 2898496
references.citation: Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1657-60
references.pmid: 11818566
references.citation: Hear Res. 1989 Oct;42(1):113-7
references.pmid: 2584156
references.citation: Hear Res. 1987;30(2-3):135-46
references.pmid: 2890617
references.citation: Hear Res. 2000 Jun;144(1-2):89-96
references.pmid: 10831868
references.citation: Hear Res. 2002 Dec;174(1-2):124-32
references.pmid: 12433403
references.citation: Neurosci Lett. 1999 Jul 2;269(1):49-51
references.pmid: 10821642
references.citation: J Neurophysiol. 2003 Nov;90(5):3178-200
references.pmid: 14615429
references.citation: Ear Hear. 1994 Dec;15(6):422-31
references.pmid: 7895938
references.citation: Eur J Neurosci. 1997 Feb;9(2):356-67
references.pmid: 9058055
references.citation: Physiol Rev. 2001 Jul;81(3):1305-52
references.pmid: 11427697
references.citation: J Assoc Res Otolaryngol. 2002 Dec;3(4):457-78
references.pmid: 12486600
references.citation: Hear Res. 2004 Jan;187(1-2):122-30
references.pmid: 14698093
references.citation: J Chromatogr. 1987 Jul 10;398:279-87
references.pmid: 3498735
references.citation: Hear Res. 1992 Dec;64(1):1-5
references.pmid: 1362722
references.citation: Hear Res. 1992 Aug;61(1-2):106-16
references.pmid: 1326504
references.citation: Hear Res. 2003 Nov;185(1-2):65-76
references.pmid: 14599694
references.citation: Noise Health. 2003 Jul-Sep;5(20):1-17
references.pmid: 14558888
references.citation: J Acoust Soc Am. 1992 Nov;92(5):2662-82
references.pmid: 1479129
references.citation: J Acoust Soc Am. 2004 Aug;116(2):1044-56
references.pmid: 15376671
references.citation: Hear Res. 1988 May;33(2):97-113
references.pmid: 3397330
references.citation: J Assoc Res Otolaryngol. 2001 Sep;2(3):268-78
references.pmid: 11669399
references.citation: Behav Brain Res. 2001 Sep 28;124(1):9-18
references.pmid: 11423161
references.citation: J Neurophysiol. 2003 Nov;90(5):2941-9
references.pmid: 12904337
references.citation: Hear Res. 1999 Dec;138(1-2):65-72
references.pmid: 10575115
references.citation: Toxicol Lett. 1989 Aug;48(2):121-49
references.pmid: 2672418
references.citation: Nature. 1961 Dec 30;192:1263-5
references.pmid: 13885734
references.citation: Brain Res Mol Brain Res. 2000 Mar 10;76(1):25-35
references.pmid: 10719212
references.citation: Hear Res. 1993 Jun;68(1):97-106
references.pmid: 8376219
references.citation: Hear Res. 1997 May;107(1-2):46-52
references.pmid: 9165346
references.citation: Acta Otolaryngol. 1995 Mar;115(2):222-6
references.pmid: 7610809
references.citation: Hear Res. 1991 Sep;55(1):133-42
references.pmid: 1684359
references.citation: Hear Res. 1997 Jun;108(1-2):89-111
references.pmid: 9213126
references.citation: Psychopharmacol Bull. 1984 Summer;20(3):548-53
references.pmid: 6332333
references.citation: Hear Res. 2003 Nov;185(1-2):97-108
references.pmid: 14599697
references.citation: J Neurol Sci. 1988 Aug;86(1):97-110
references.pmid: 3262717
references.citation: J Neurosci. 1996 Jan;16(1):325-32
references.pmid: 8613799
references.citation: ORL J Otorhinolaryngol Relat Spec. 1993;55(1):3-6
references.pmid: 8382781
references.citation: Hear Res. 1987;25(1):69-76
references.pmid: 3804858
references.citation: Ann N Y Acad Sci. 1999 Nov 28;884:292-304
references.pmid: 10842601
references.citation: Hear Res. 1998 Nov;125(1-2):147-53
references.pmid: 9833968
references.citation: Int J Dev Neurosci. 1997 Jul;15(4-5):631-43
references.pmid: 9263039
references.citation: Br J Audiol. 1994 Aug-Oct;28(4-5):185-91
references.pmid: 7735146
references.citation: Mov Disord. 1998;13 Suppl 1:35-8
references.pmid: 9613716
references.citation: J Acoust Soc Am. 1996 Jun;99(6):3572-84
references.pmid: 8655789
references.citation: Acta Otolaryngol Suppl. 1988;447:36-45
references.pmid: 2903609
references.citation: Acta Otolaryngol. 1995 Jan;115(1):27-30
references.pmid: 7762379
references.citation: Anat Embryol (Berl). 1992;185(1):1-16
references.pmid: 1736680
references.citation: Hear Res. 2004 Apr;190(1-2):75-86
references.pmid: 15051131
references.citation: J Assoc Res Otolaryngol. 2003 Jun;4(2):276-90
references.pmid: 12943378
references.citation: Hear Res. 2001 May;155(1-2):143-51
references.pmid: 11335084
references.citation: Acta Otolaryngol. 1974 Jan-Feb;77(1):56-65
references.pmid: 4829054
references.citation: Exp Brain Res. 2003 Dec;153(4):499-513
references.pmid: 14557913
references.citation: J Neurosci. 1995 May;15(5 Pt 1):3667-78
references.pmid: 7751937
references.citation: Hear Res. 1987;29(2-3):179-94
references.pmid: 3624082
references.citation: Neuroreport. 1997 Feb 10;8(3):591-6
references.pmid: 9106729
references.citation: Neuroscience. 1983 Jul;9(3):621-30
references.pmid: 6353274
references.citation: J Physiol. 1975 May;247(2):407-28
references.pmid: 1097639
references.citation: J Neurosci. 2000 Jun 15;20(12):4701-7
references.pmid: 10844039
references.citation: Hear Res. 1999 Aug;134(1-2):57-70
references.pmid: 10452376
references.citation: Neuroscience. 2001;106(3):589-601
references.pmid: 11591459
references.citation: Eur Arch Otorhinolaryngol. 1995;252(5):270-4
references.pmid: 7576583
references.citation: Am J Otol. 1996 May;17(3):486-92
references.pmid: 8817030
references.citation: Eur J Neurosci. 1992;4(10):981-992
references.pmid: 12106433
references.citation: Behav Brain Res. 2001 Nov 1;125(1-2):109-25
references.pmid: 11682102
references.citation: Hear Res. 1998 Dec;126(1-2):67-74
references.pmid: 9872135
references.citation: Hear Res. 1997 Jun;108(1-2):74-82
references.pmid: 9213124