Participation of Rhipicephalus sanguineus (Acari: Ixodidae) in the epidemiology of canine visceral leishmaniasis.
The vectorial competence of the tick Rhipicephalus sanguineus is discussed in relation to the epidemiology of canine visceral leishmaniasis, taking into account its strict association with dogs and the low indices of natural infection presented by its known vector, the phlebotomine sand fly Lutzomyia longipalpis. In order to evaluate natural infection by Leishmania chagasi and the infectivity of these parasites in the tick, 39 specimens (6 females, 11 males and 22 nymphs) of R. sanguineus were removed from 21 dogs showing diverse symptoms of zoonotic visceral leishmaniasis (ZVL). Six ticks (15.4%) gave positive results for the genus Leishmania using the PCR technique. To determine the infectivity of the parasites, 36 hamsters were inoculated orally and peritoneally with macerates of ticks removed from nine dogs symptomatic for visceral leishmaniasis. After 6 months the hamsters were sacrificed and necropsied. Serum was removed for IFAT, as well as spleen and liver fragments to make imprint smears and for PCR. Eight (88.9%) of these dogs presented ticks that were infective for 14 hamsters (41.2%), 12 (85.7%) of them infected peritoneally and two (14.3%) orally. PCR revealed 27 smears (40.9%) to be positive, 20 (62.5%) of them infected peritoneally and seven (20.6%) orally. IFAT showed 14 positive animals (41.2%). Based on these findings, we suggest that the vectorial capacity of R. sanguineus for L. chagasi should be evaluated further, opening new perspectives in the epidemiology of ZVL.
Coutinho
Maria Teresa Zanatta
MT
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Caixa Postal 486, Avenida Antônio Carlos, 6627, Campus UFMG, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil.
Bueno
Lilian Lacerda
LL
Sterzik
Annelise
A
Fujiwara
Ricardo Toshio
RT
Botelho
Jose Ramiro
JR
De Maria
Mario
M
Genaro
Odair
O
Linardi
Pedro Marcos
PM
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001179:Arthropod Vectors / Q000469:parasitology; D001938:Brazil / Q000453:epidemiology; D006224:Cricetinae; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology* / Q000635:transmission; D004285:Dogs; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D026863:Ixodidae / Q000469:parasitology*; D018314:Leishmania infantum / Q000254:growth & development*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission / Q000662:veterinary*; D008099:Liver / Q000469:parasitology; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000469:parasitology; D013984:Tick Infestations / Q000469:parasitology; D015047:Zoonoses / Q000469:parasitology*
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan