[Simultaneous analysis of theophylline, phenobarbital, amobarbital and carbamazepine in serum by high performance liquid chromatography].
Theophylline, phenobarbital, amobarbital and carbamazepine are the chief therapeutic drugs in clinical practice. Because of the differences among inter-individuals in the metabolic clearance of these drugs and their toxicity at certain levels of concentration in serum, the dosage should be regulated to maintain a therapeutic blood drugs level. To achieve this a rapid and accurate assay method for drugs in blood is necessary. This paper reports that a reversed phase high performance liquid chromatographic (HPLC) method had been established for the simultaneous separation and quantitative determination of the four drugs in serum. The separation was performed on RP-C18 column using methanol:water (1:1) as mobile phase and 4-aminoantipyrime as internal standard. The eluted substances were detected at 210nm.
Fu
S
S
China Medical University, Shenyang, 110031.
Duan
S
S
Ren
Q
Q
D000654:Amobarbital / Q000097:blood*; D002220:Carbamazepine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010634:Phenobarbital / Q000097:blood*; D013806:Theophylline / Q000097:blood*
D002220:Carbamazepine; D013806:Theophylline; D000654:Amobarbital; D010634:Phenobarbital