Evaluation of the antioxidant and free radical scavenging properties of Secamone afzelii Rhoem.
The antioxidant activity of a methanol extract of Secamone afzelii stems was tested using the DPPH assay and the active compound was identified as alpha-tocopherol. HPLC determination showed that 0.12% w/w alpha-tocopherol was present in the plant. The total extract also showed effective free radical scavenging activity in the assay for non-enzymatic lipid peroxidation in liposomes with an IC50 value of 90 microg/mL, with alpha-tocopherol isolated from the plant having an IC50 of 15 microg/mL in the same system, thus demonstrating the presence of other antioxidants.
Mensah
A Y
AY
Department of Pharmacognosy, Faculty of Pharmacy, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Houghton
P J
PJ
Akyirem
G N A
GN
Fleischer
T C
TC
Mensah
M L K
ML
Sarpong
K
K
Adosraku
R
R
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D029062:Apocynaceae; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000378:metabolism; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018547:Plant Stems
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D008081:Liposomes; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl