Model of murine interpositional vein grafting.
A new murine model is presented for interpositional vein grafting into an artery. In ICR (outbred) mice, the femoral vein or a branch of the jugular vein was harvested and placed into a femoral artery defect, using standard microvascular techniques to achieve end-to-end anastomoses with 11-0 nylon suture. The technique requires a high level of microsurgical experience and competence. Success rates (vessel patency) were higher with moderate systemic heparinization (200 units/kg body weight, single i.v. bolus). Vein grafts were successfully transplanted between syngenic C57Bl/6J mice, with patency demonstrated from 1 day to 3 months postoperatively. The model is applicable to studies of vein graft stenosis using murine transgenic/knockout models.
Cooley
Brian C
BC
Department of Orthopaedic Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA. bcooley@mcw.edu
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D005260:Female; D006083:Graft Occlusion, Vascular / Q000601:surgery; D008297:Male; D051379:Mice; D008866:Microsurgery / Q000379:methods; D023421:Models, Animal; D013536:Suture Techniques; D014656:Vascular Surgical Procedures / Q000379:methods*; D014680:Veins / Q000637:transplantation*