title: Expression of protein kinases C betaI, betaII, and VEGF during the differentiation of enamel epithelium in tooth development.
abstract: Protein kinase C (PKC) is an important molecule involved in various cell function, and mediates induced secretion of vascular endothelial growth factor (VEGF). It is hypothesized that PKC and VEGF may be associated with tooth development. Using the laser microdissection method and real-time reverse-transcription-polymerase chain-reaction (RT-PCR), we investigated the expression of PKC betaI and betaII, VEGF, and amelogenin (used as a marker of differentiation to ameloblasts) in the inner and outer enamel epithelia, stellate reticulum, and dental papilla in each stage of the dental germ. We found that the expression levels of PKC betaI and betaII were increased in the inner enamel epithelium during the early bell stage. In addition, the increased expression levels of PKC betaI and betaII were accompanied by increased VEGF expression. These results indicate that PKC betaI, betaII, and VEGF are closely associated with the differentiation of the inner enamel epithelium to ameloblasts.
authors.affiliation: Department of Oral Pathology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. aida@dent.showa-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Aida
authors.forename: T
authors.initials: T
authors.lastname: Irié
authors.forename: T
authors.initials: T
authors.lastname: Aida
authors.forename: T
authors.initials: T
authors.lastname: Tachikawa
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000201:enzymology; D053523:Amelogenin; D000818:Animals; D002454:Cell Differentiation; D003746:Dental Enamel Proteins / Q000032:analysis; D003771:Dental Papilla / Q000166:cytology / Q000201:enzymology; D004658:Enamel Organ / Q000166:cytology* / Q000201:enzymology; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000032:analysis*; D007834:Lasers; D042282:Microdissection; D009805:Odontogenesis / Q000502:physiology*; D011493:Protein Kinase C / Q000032:analysis*; D064546:Protein Kinase C beta; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014083:Tooth Germ / Q000166:cytology / Q000201:enzymology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: D053523:Amelogenin; C507201:Amelx protein, rat; D003746:Dental Enamel Proteins; D007527:Isoenzymes; D042461:Vascular Endothelial Growth Factor A; D011493:Protein Kinase C; D064546:Protein Kinase C beta