title: [Expression of VEGF-C and VEGFR-3 in human laryngeal squamous cell carcinomas and cervical metastatic lymph node tissues].
abstract: OBJECTIVE
  To study the gene expression of vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) in laryngeal squamous carcinoma and their relation with clinic pathologic features.
  
  
  METHODS
  VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma (PLC) and cervical lymph node (CLN) were examined by reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The gene expression of VEGF-C and VEGFR-3 are different among normal laryngeal mucosa tissue, primary laryngeal carcinoma and cervical lymph node in the same patient. In primary laryngeal carcinoma, expression of VEGF-C, VEGFR-3 were significantly higher in lymph node positive group than in lymph node negative group and associated with histological grade of differentiation; The gene expression of VEGF-C, VEGFR-3 were not associated with age, sex, site, T stage.
  
  
  CONCLUSIONS
  VEGF-C, VEGFR-3 have prognostic significance in laryngeal carcinoma.
authors.affiliation: Department of Human Anatomy, West China of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.forename: Chuanyu
authors.initials: C
authors.lastname: Liang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000556:secondary; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009333:Neck; D011379:Prognosis; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000096:biosynthesis*
chemical_list: D042582:Vascular Endothelial Growth Factor C; D040321:Vascular Endothelial Growth Factor Receptor-3