RIN13 is a positive regulator of the plant disease resistance protein RPM1.
The RPM1 protein confers resistance to Pseudomonas syringae pv tomato DC3000 expressing either of the Type III effector proteins AvrRpm1 or AvrB. Here, we describe the isolation and functional characterization of RPM1 Interacting Protein 13 (RIN13), a resistance protein interactor shown to positively enhance resistance function. Ectopic expression of RIN13 (RIN13s) enhanced bacterial restriction mechanisms but paradoxically abolished the normally rapid hypersensitive response (HR) controlled by RPM1. In contrast with wild-type plants, leaves expressing RIN13s did not undergo electrolyte leakage or accumulate H2O2 after bacterial delivery of AvrRpm1. Overexpression of RIN13 also altered the transcription profile observed during a normal HR. By contrast, RIN13 knockout plants had the same ion leakage signatures and HR timing of wild-type plants in response to DC3000(avrRpm1) but failed to suppress bacterial growth. The modified phenotypes seen in the RIN13s/as plants were specific to recognition of AvrRpm1 or AvrB, and wild-type responses were observed after challenge with other incompatible pathogens or the virulent DC3000 isolate. Our results suggest that cell death is not necessary to confer resistance, and engineering enhanced resistance without activation of programmed cell death is a real possibility.
Al-Daoude
Antonious
A
Department of Agricultural Science, Imperial College, London, TN25 5AH United Kingdom.
de Torres Zabala
Marta
M
Ko
Jong-Hyun
JH
Grant
Murray
M
D000595:Amino Acid Sequence; D017209:Apoptosis; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000382:microbiology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D018744:DNA, Plant / Q000235:genetics; D017353:Gene Deletion; D017343:Genes, Plant; D008954:Models, Biological; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D018744:DNA, Plant; C095014:RPM1 protein, Arabidopsis; D011994:Recombinant Proteins
Science. 2003 Aug 29;301(5637):1230-3
12947197
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):8024-9
12788974
Cell. 2002 Mar 22;108(6):743-54
11955429
Cell. 2003 Feb 7;112(3):369-77
12581526
Trends Biochem Sci. 1999 Feb;24(2):47-53
10098397
Plant J. 2001 Apr;26(1):101-12
11359614
Dev Cell. 2002 Jun;2(6):807-17
12062092
Plant Cell. 2003 Feb;15(2):365-79
12566578
Science. 2003 Aug 1;301(5633):653-7
12893945
Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3747-52
10725385
J Biol Chem. 2004 Jan 16;279(3):2101-8
14583611
Curr Opin Biotechnol. 2003 Apr;14(2):177-93
12732319
Plant J. 1998 Dec;16(6):735-43
10069079
Mol Plant Microbe Interact. 2005 Feb;18(2):95-102
15720077
Nat Rev Mol Cell Biol. 2003 Feb;4(2):95-104
12563287
Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):517-22
11756663
Microbes Infect. 2003 Apr;5(4):301-10
12706443
Nat Rev Immunol. 2003 May;3(5):371-82
12766759
Cell. 2003 Feb 7;112(3):379-89
12581527
Mol Plant Microbe Interact. 1995 Nov-Dec;8(6):916-28
8664502
Plant J. 2000 Aug;23(4):441-50
10972870
Curr Biol. 2004 Jan 6;14(1):R22-4
14711430
Plant Cell. 2000 Aug;12(8):1367-77
10948256
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6597-601
11607554
Nature. 2001 Jun 14;411(6839):826-33
11459065
Plant Cell. 2002 May;14(5):979-92
12034891
Annu Rev Plant Biol. 2003;54:23-61
14502984
Plant Cell. 2002 May;14(5):1005-15
12034893
EMBO J. 2000 Aug 1;19(15):4004-14
10921881
Plant J. 2003 Feb;33(4):665-76
12609040
Plant Cell. 2003 Apr;15(4):806-7
12671078
EMBO J. 2002 Sep 2;21(17):4511-9
12198153
Oncogene. 2001 Oct 1;20(44):6473-81
11607846
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15843-8
9861058
Curr Opin Cell Biol. 2002 Dec;14(6):715-20
12473344
Science. 1995 Aug 11;269(5225):843-6
7638602
Plant Physiol. 1998 Nov;118(3):1067-78
9808752
Curr Biol. 1998 Mar 26;8(7):R226-7
9545207
Mol Microbiol. 1994 Jun;12(6):941-50
7934901
Cell. 1999 Nov 12;99(4):355-66
10571178
Plant Cell. 2003 Feb;15(2):317-30
12566575
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9323-8
10900264
Plant Cell. 2002 Feb;14(2):435-50
11884685
Trends Plant Sci. 2003 Jun;8(6):252-8
12818658
Plant Physiol. 2000 Sep;124(1):21-9
10982418
Genetics. 2001 May;158(1):439-50
11333251
Plant Cell. 2003 Apr;15(4):809-34
12671079
Science. 1997 Dec 12;278(5345):1963-5
9395402
Curr Opin Plant Biol. 1999 Aug;2(4):312-9
10458998
Curr Opin Plant Biol. 2003 Aug;6(4):312-9
12873524
Mol Microbiol. 2003 Jul;49(2):377-87
12828636
J Bacteriol. 1993 Aug;175(15):4859-69
8335641
Plant Cell. 1990 May;2(5):437-45
2152169
Curr Opin Struct Biol. 2001 Dec;11(6):725-32
11751054
Curr Opin Microbiol. 2002 Feb;5(1):44-50
11834368
Trends Biochem Sci. 1998 Dec;23(12):454-6
9868361
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15849-54
9861059
Plant Cell. 2002 Nov;14(11):2929-39
12417711