A mutation in dVps28 reveals a link between a subunit of the endosomal sorting complex required for transport-I complex and the actin cytoskeleton in Drosophila.
Proteins that constitute the endosomal sorting complex required for transport (ESCRT) are necessary for the sorting of proteins into multivesicular bodies (MVBs) and the budding of several enveloped viruses, including HIV-1. The first of these complexes, ESCRT-I, consists of three proteins: Vps28p, Vps37p, and Vps23p or Tsg101 in mammals. Here, we characterize a mutation in the Drosophila homolog of vps28. The dVps28 gene is essential: homozygous mutants die at the transition from the first to second instar. Removal of maternally contributed dVps28 causes early embryonic lethality. In such embryos lacking dVps28, several processes that require the actin cytoskeleton are perturbed, including axial migration of nuclei, formation of transient furrows during cortical divisions in syncytial embryos, and the subsequent cellularization. Defects in actin cytoskeleton organization also become apparent during sperm individualization in dVps28 mutant testis. Because dVps28 mutant cells contained MVBs, these defects are unlikely to be a secondary consequence of disrupted MVB formation and suggest an interaction between the actin cytoskeleton and endosomal membranes in Drosophila embryos earlier than previously appreciated.
Sevrioukov
Evgueni A
EA
Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9111, USA.
Moghrabi
Nabil
N
Kuhn
Mary
M
Krämer
Helmut
H
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D005260:Female; D017344:Genes, Insect; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D013091:Spermatogenesis; D033921:Vesicular Transport Proteins / Q000235:genetics* / Q000378:metabolism*
D000199:Actins; D029721:Drosophila Proteins; D033921:Vesicular Transport Proteins
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7626-31
12802020
Mech Dev. 1995 Apr;50(2-3):201-16
7619731
Curr Opin Cell Biol. 1998 Aug;10(4):513-22
9719873
Nat Med. 2003 Oct;9(10):1262-3
14520374
Cell. 1991 Dec 20;67(6):1145-55
1760842
Semin Cell Dev Biol. 2002 Apr;13(2):91-7
12127141
Curr Biol. 2003 Oct 14;13(20):R813-4
14561424
Mech Dev. 2000 Mar 1;91(1-2):451-4
10704882
Genetics. 1999 Feb;151(2):675-84
9927460
Development. 2002 Jan;129(2):517-26
11807042
Mol Biol Cell. 2003 Jul;14(7):2908-20
12857874
Dev Biol. 1998 Dec 1;204(1):151-64
9851849
Genetics. 1996 Dec;144(4):1673-9
8978054
J Virol. 2003 Apr;77(8):4794-804
12663786
Genome Biol. 2002;3(12):RESEARCH0080
12537569
Cell. 2001 Oct 5;107(1):55-65
11595185
Cell. 2001 Jul 27;106(2):145-55
11511343
Traffic. 2004 Mar;5(3):194-210
15086794
J Cell Biol. 2002 Nov 11;159(3):477-87
12427870
J Biol Chem. 2004 Aug 20;279(34):36059-71
15218037
Mol Biol Cell. 2002 Jul;13(7):2486-501
12134085
Methods Cell Biol. 1994;44:489-505
7707968
Curr Opin Neurol. 1997 Oct;10(5):436-42
9330892
Development. 1998 May;125(10):1833-43
9550716
Cell. 1987 Oct 9;51(1):151-8
3115594
Genetics. 1999 Sep;153(1):135-77
10471706
J Virol. 2003 Jun;77(11):6507-19
12743307
Development. 1998 Apr;125(7):1295-303
9477328
Dev Cell. 2002 Aug;3(2):271-82
12194857
Development. 2003 Aug;130(16):3725-33
12835389
Development. 2003 May;130(9):1805-16
12642486
Development. 2001 Mar;128(6):815-26
11222137
Traffic. 2001 Sep;2(9):612-21
11555415
Development. 1995 Jun;121(6):1937-46
7601006
J Cell Biol. 2003 Oct 13;163(1):143-54
14530382
Cell. 2003 Sep 19;114(6):701-13
14505570
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1859-64
11172041
J Cell Biol. 1996 Mar;132(6):1011-23
8601581
Nat Rev Mol Cell Biol. 2004 Apr;5(4):317-23
15071556
Dev Biol. 2001 Sep 15;237(2):282-94
11543614
Mol Biol Cell. 2004 Sep;15(9):4337-46
15240819
Genetics. 1999 Aug;152(4):1631-9
10430588
J Cell Biol. 2003 Aug 4;162(3):435-42
12900395
Dev Cell. 2002 Aug;3(2):283-9
12194858
Nat Cell Biol. 1999 May;1(1):72-4
10559868
Mol Biol Cell. 2004 Jan;15(1):345-58
14617814
Development. 1993 Apr;117(4):1223-37
8404527
Mol Biol Cell. 2000 Mar;11(3):1011-22
10712516
J Cell Biol. 2000 May 15;149(4):901-14
10811830
J Cell Biol. 1996 Jul;134(1):133-48
8698810
Annu Rev Cell Dev Biol. 2003;19:287-332
14570572
Development. 1991 Apr;111(4):909-20
1879360
J Cell Biol. 1996 Aug;134(4):923-34
8769417
J Biol Chem. 2001 Apr 13;276(15):11735-42
11134028
J Cell Sci. 1999 Sep;112 ( Pt 17):2885-93
10444383
J Cell Biol. 1994 Dec;127(6 Pt 1):1637-53
7798318
Nat Rev Mol Cell Biol. 2002 Dec;3(12):893-905
12461556
J Cell Biol. 2002 Aug 19;158(4):669-79
12177043
Curr Biol. 2003 Oct 28;13(21):1848-57
14588240
Nucleic Acids Res. 1988 Feb 11;16(3):1043-61
3125519
EMBO J. 1993 Jul;12(7):2855-62
8335001
Mol Cell Biol. 2003 Jan;23(1):150-62
12482969
J Cell Biol. 2003 Aug 4;162(3):425-34
12900394
Nature. 1991 Jul 18;352(6332):207-12
1857416
Mol Biol Cell. 1996 Jun;7(6):985-99
8817003
Cell. 1997 Oct 3;91(1):109-18
9335339
Genes Dev. 2002 Jun 1;16(11):1314-36
12050111
Mol Biol Cell. 1998 Apr;9(4):809-16
9529379
Dev Cell. 2003 Jul;5(1):139-48
12852858
Mol Cell. 1999 Oct;4(4):479-86
10549280
Curr Opin Cell Biol. 2003 Aug;15(4):446-55
12892785
Mol Biol Cell. 2004 Feb;15(2):838-50
14657248
Cell. 2003 Nov 14;115(4):475-87
14622601
J Cell Sci. 2002 Dec 15;115(Pt 24):4855-65
12432073
Genetics. 1988 Mar;118(3):461-70
2835286
J Cell Biol. 2003 Aug 4;162(3):413-23
12900393
Science. 2004 Jan 23;303(5657):531-4
14739459
Nature. 1996 Oct 17;383(6601):627-30
8857539
Cell. 2002 Jan 25;108(2):261-9
11832215
Development. 1993 Jun;118(2):401-15
8223268
Traffic. 2002 Feb;3(2):87-91
11929598
J Cell Biol. 2002 Apr 1;157(1):91-101
11916981
Science. 2000 Mar 24;287(5461):2185-95
10731132
Genes Dev. 2000 Jul 1;14(13):1651-63
10887159
Mech Dev. 1991 Jun;34(2-3):101-11
1911395
Mol Biol Cell. 1993 Sep;4(9):885-96
8257792
Mol Gen Genet. 2000 Jul;263(6):1003-14
10954086
Mol Biol Cell. 2001 Nov;12(11):3451-64
11694580
EMBO J. 1997 Aug 1;16(15):4508-18
9303295
Curr Biol. 2002 Jan 22;12(2):R74-8
11818085
Traffic. 2000 Mar;1(3):248-58
11208108
Dev Biol. 1999 Jan 1;205(1):129-44
9882502
J Biol Chem. 2002 Nov 8;277(45):43216-23
12205095
J Cell Biol. 1996 Jun;133(6):1205-15
8682859
Trends Cell Biol. 1998 Jul;8(7):257-9
9714595
Mol Biol Cell. 1999 Apr;10(4):847-59
10198042
Cell. 1998 Dec 11;95(6):847-58
9865702