Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling.
Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2C12 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-to-adipocyte differentiation significantly, concurrent with an enhanced expression of Wnt10b mRNA. Inhibition of the Wnt signaling by incubation of the myoblasts with a soluble Wnt ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Wnt signaling.
Akimoto
Takayuki
T
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan. takayuki.akimoto@duke.edu
Ushida
Takashi
T
Miyaki
Shigeru
S
Akaogi
Hiroshi
H
Tsuchiya
Kohei
K
Yan
Zhen
Z
Williams
R Sanders
RS
Tateishi
Tetsuya
T
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D002462:Cell Membrane / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D032446:Myoblasts / Q000166:cytology*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013314:Stress, Mechanical; D051153:Wnt Proteins
D001391:Azo Compounds; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D012333:RNA, Messenger; D051153:Wnt Proteins; D012313:RNA; C011049:oil red O