HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor.
Heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120 depends on both the global nitrogen control transcription factor NtcA and the cell differentiation regulatory protein HetR, with expression of ntcA and hetR being dependent on each other. In this study we constructed strains that constitutively express the ntcA gene leading to high levels of NtcA protein irrespective of the nitrogen source, and we analyzed the effects of such NtcA levels on heterocyst differentiation. In the NtcA-overproducing strain, heterocyst differentiation, induction of NtcA-dependent heterocyst development genes or operons such as devBCA or the cox2 operon, and NtcA-dependent excision of the 11-kb nifD-intervening element only took place under nitrogen deficiency. Although functional heterocysts were produced in response to nitrogen step-down, the NtcA overproducing strain could not grow diazotrophically. Overexpression of ntcA in a hetR background promoted expression of devBCA in response to ammonium withdrawal and excision of the 11-kb element even in the presence of combined nitrogen. Our results show that some NtcA-dependent heterocyst-related genes can be expressed independently of HetR.
Olmedo-Verd
Elvira
E
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas, Universidad de Sevilla, Seville, Spain.
Flores
Enrique
E
Herrero
Antonia
A
Muro-Pastor
Alicia M
AM
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D017033:Anabaena / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D051546:Cyclooxygenase 2; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009584:Nitrogen / Q000378:metabolism; D009591:Nitrogenase / Q000235:genetics; D009876:Operon / Q000502:physiology; D010088:Oxidoreductases / Q000235:genetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C091089:DevA protein, Anabaena; D014157:Transcription Factors; C067403:HetR protein, Bacteria; D010088:Oxidoreductases; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D009591:Nitrogenase; C029130:nitrogenase reductase; D009584:Nitrogen
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