title: Genetic variability of 16 Y-chromosome STRs in a sample from Equatorial Guinea (Central Africa).
abstract: Nine Y-STR loci from the "minimal haplotype" included in Y-STR Haplotype Reference Databases (YHRD) together with eight additional Y-STRs (DYS437, DYS438, DYS439, DYS460, DYS461, GATA C4, GATA H4 and GATA A10) were analyzed in a sample of 101 males from Equatorial Guinea living in Madrid. Haplotype and allelic frequencies were calculated and genetic diversities were estimated for each genetic system as well as for the whole haplotype. An unexpected high frequency (6%) of intermediate alleles (13.2 and 14.2) was found in DYS385. For DYS19, two alleles were found in one sample. Another sample failed to amplify with DYS393 primers using either PowerPlex Y System (Promega Corporation) or the Y-PLEXtrade mark 12 (Reliagene, New Orleans, LA) commercial kits. Comparison between Equatorial Guinea and another African population (Mozambique; South East Coast) revealed a significant pairwise Phi(st) value between them (Phi(st)=0.03309; P=0.00000).
authors.affiliation: Departamento de Toxicología y Legislación Sanitaria, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. earroyop@med.ucm.es
authors.forename: Eduardo
authors.initials: E
authors.lastname: Arroyo-Pardo
authors.forename: Leonor
authors.initials: L
authors.lastname: Gusmão
authors.forename: Ana María
authors.initials: AM
authors.lastname: López-Parra
authors.forename: Carlos
authors.initials: C
authors.lastname: Baeza
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Mesa
authors.forename: António
authors.initials: A
authors.lastname: Amorim
mesh_terms: D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000379:methods; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D016701:Guinea / Q000208:ethnology; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013030:Spain; D020080:Tandem Repeat Sequences