title: Natural recombinant between equine herpesviruses 1 and 4 in the ICP4 gene.
abstract: Equine herpesvirus 1 (EHV-1) is a pathogen causing rhinopneumonia in young horses, abortion in mares, and myeloencephalitis in adult horses. Two types, EHV-1 P and EHV-1 B, have recently been dominant among 16 electropherotypes. EHV-1 P and EHV-1 B viruses were compared by long and accurate polymerase chain reaction (LA-PCR) and restriction fragment length polymorphism (RFLP) analysis. Differences in restriction sites were found to be focused in ORF64, which encodes the infected cell protein 4 (ICP4), and downstream of the ICP4 gene. The 3 ' -end and downstream of ICP4 gene of EHV-1 B were found to be replaced by the corresponding region of EHV-4, indicating that EHV-1 B is a naturally occurring recombinant virus between progenitors of EHV-1 P and EHV-4. This is the first report showing a natural interspecies recombinant in alphaherpesviruses.
authors.affiliation: Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Japan.
authors.forename: Ochir
authors.initials: O
authors.lastname: Pagamjav
authors.forename: Tohru
authors.initials: T
authors.lastname: Sakata
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Hideto
authors.initials: H
authors.lastname: Fukushi
mesh_terms: D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D006566:Herpesviridae Infections / Q000473:pathology / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000302:isolation & purification; D023141:Herpesvirus 4, Equid / Q000235:genetics* / Q000302:isolation & purification; D006736:Horses; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins