[Effect of Eucalyptus globulus oil on lipopolysaccharide-induced chronic bronchitis and mucin hypersecretion in rats].
OBJECTIVE
To study the effect of Eucalyptus globulus oil on bronchiolitis and mucin hypersecretion in chronic bronchitis induced by lipopolysaccharide in rats.


METHODS
Rat model was established by intratracheal instillation of lipopolysaccharide 0.2 mg. Pathological changes, alteration in bronchoalveolar lavage fluid (BALF) and immunohistochemistry characters were examined after 3 weeks and the effect of E. globulus oil was observed.


RESULTS
Characters of pathological manifestations of chronic bronchitis were found after instillation of LPS. Inflammatory cell infiltration and bronchiolitis severity were significantly reduced after administration of E. globulus oil. Especially in 300 mg x kg(-1) treated rats, there were significant decreases of mucin content in BALF and MUC5ac expression in trachea and bronchiole epithelium. Optical density and mucins area% detected by image analysis system were apparently lower than those in model group.


CONCLUSIONS
E. globulus oil has the anti-inflammatory effect on chronic bronchitis induced by lipopolysaccharide in rats and the inhibitio effect on hypersecretion of airway mucins.
Lu
Xiao-qin
XQ
Zhejiang Respiratory Drugs Research Laboratory of State Food and Drugs Administration of China, College of Medical Sciences, Zhejiang University, Hangzhou 310031, China. lvxiaoqing272@sohu.com
Tang
Fa-di
FD
Wang
Yan
Y
Zhao
Ting
T
Bian
Ru-lian
RL
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D029481:Bronchitis, Chronic / Q000139:chemically induced / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D005052:Eucalyptus / Q000737:chemistry; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011135:Polysaccharides, Bacterial; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009077:Mucins; D009822:Oils, Volatile; D011135:Polysaccharides, Bacterial