[Effect of Shenluotong on secretion of ECM and expression of TGF-beta1 in rats mesangial cells of rats].
OBJECTIVE
To investigate the effect of Shenluotong on the expression of transforming growth factor-beta1 (TGF-beta1) and extracellular matrix (ECM) in Ang II-induced MCs.


METHODS
Fibronectin (FN) and collagen type IV (Col IV) of extracellular matrix were detected by enzyme-linked immunosorbent assay; the expression of TGF-beta1 mRNA were measured by semi-quantitative reverse transcription-polymerase chain reaction.


RESULTS
A positive correlation between TGF-beta1 and ECM were found in the present study. FN, Col IV and TGF-beta1 mRNA were inhibited by Shenluotong significantly.


CONCLUSIONS
Shenluotong can decrease the accumulation of ECM and inhibit the expression of TGF-beta1, suggesting further that shenluotong can be used to prevent and treat various glomerular diseases and delay glomerular sclerosis.
Wang
Ya-li
YL
Traditional Chinese Medicine college, Hebei Medical University, shijiazhuang 050091, China.
Zhao
Yu-yong
YY
Chen
Zhi-qiang
ZQ
Xu
Qing-you
QY
Xu
Hua-zhou
HZ
Li
Tong-zhong
TZ
Hou
Xian-ming
XM
D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D005920:Glomerular Mesangium / Q000166:cytology*; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D009835:Oligochaeta / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1
D024141:Collagen Type IV; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D008419:Materia Medica; D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1