title: [Determination of ligustilide in volatile oil from rhizome of ligusticum chuanxiong by RP-HPLC].
abstract: OBJECTIVE
  To determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC.
  
  
  METHODS
  ODS2 column (4.6 mm x 200 mm, 5 microm) was used and nitrendipine was used as internal standard. The mobil phase consisted methanol, acetontrile and water (33:21:46). The ligustilide was at 275 nm.
  
  
  RESULTS
  The linear range was 2.92-29.2 mg x L(-1) for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%.
  
  
  CONCLUSIONS
  This method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Yan-zhi
authors.initials: YZ
authors.lastname: Lin
authors.forename: Xing
authors.initials: X
authors.lastname: Tang
authors.forename: Kai-shun
authors.initials: KS
authors.lastname: Bi
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030004:Ligusticum / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
chemical_list: D009822:Oils, Volatile; C027820:ligustilide; D015107:4-Butyrolactone