Radical species in DNA strand-cleavage caused by dihydropyrazines.
Dihydropyrazines (DHPs), which generate hydroxyl and carbon-centered radicals, cleaved DNA single-strand. It is new knowledge that DHPs were recently determined to produce 8-hydroxydeoxyguanosine. The remarkable increase in the DNA strand-cleavage activity upon the addition of Cu2+ suggests that the primary reactive species is carbon-centered radicals rather than the hydroxyl radical generated the initiation reaction. This proposal was in fair agreement with of the observed carbon-centered radical signal intensity, but an effect was not observed with the increase in the hydroxyl radical signal intensity.
Kashige
Nobuhiro
N
Faculty of Pharmaceutical Sciences, Fukuoka University, Fokuoka, Japan.
Takeuchi
Toru
T
Matsumoto
Shigenobu
S
Takechi
Shinji
S
Miake
Fumio
F
Yamaguchi
Tadatoshi
T
D004277:DNA, Single-Stranded / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism*; D011719:Pyrazines / Q000737:chemistry / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D005609:Free Radicals; D011719:Pyrazines