title: The past, present and future of cell-free protein synthesis.
abstract: Recent technical advances have revitalized cell-free expression systems to meet the increasing demands for protein synthesis. Cell-free systems offer several advantages over traditional cell-based expression methods, including the easy modification of reaction conditions to favor protein folding, decreased sensitivity to product toxicity and suitability for high-throughput strategies because of reduced reaction volumes and process time. Moreover, improvements in translation efficiency have resulted in yields that exceed a milligram of protein per milliliter of reaction mix. We review the advances on this expanding technology and highlight the growing list of associated applications.
authors.affiliation: Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008, USA.
authors.forename: Federico
authors.initials: F
authors.lastname: Katzen
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Chang
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Kudlicki
mesh_terms: D002474:Cell-Free System; D019020:Directed Molecular Evolution / Q000379:methods / Q000639:trends; D014176:Protein Biosynthesis; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D011994:Recombinant Proteins