title: Structural and serological characterization of the O-chain polysaccharide of Aeromonas salmonicida strains A449, 80204 and 80204-1.
abstract: The O-chain polysaccharide (O-PS) of Aeromonas salmonicida was studied by a combination of compositional, methylation, CE-ESMS and one- and two-dimensional NMR analyses. It was found to be a branched polymer of trisaccharide-repeating units composed of L-rhamnose (Rha), D-glucose (Glc), 2-acetamido-2-deoxy-D-mannose (ManNAc) and O-acetyl group (OAc) and having the following structure: CE-ESMS analysis of A. salmonicida cells from strains A449, 80204 and 80204-1 grown under different conditions confirmed that the O-PS structure was conserved. ELISA-based serological study with native LPS-specific antisera performed on the native O-PS and its O-deacetylated and periodate-oxidized derivatives confirmed the importance of the O-PS backbone structure as an immunodominant determinant.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Suzon
authors.initials: S
authors.lastname: Larocque
authors.forename: Blair A
authors.initials: BA
authors.lastname: Harrison
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Eleonora
authors.initials: E
authors.lastname: Altman
mesh_terms: D048409:Aeromonas salmonicida / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D008745:Methylation; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D012703:Serotyping; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D019081:O Antigens