title: Electrophysiological and molecular identification of hepatocellular volume-activated K+ channels.
abstract: Although K+ channels are essential for hepatocellular function, it is not known which channels are involved in the regulatory volume decrease (RVD) in these cells. We have used a combination of electrophysiological and molecular approaches to describe the potential candidates for these channels. The dialysis of short-term cultured rat hepatocytes with a hypotonic solution containing high K+ and low Cl- concentration caused the slow activation of an outward, time-independent current under whole-cell configuration of the patch electrode voltage clamp. The reversal potential of this current suggested that K+ was the primary charge carrier. The swelling-induced K+ current (IKvol) occurred in the absence of Ca2+ and was inhibited with 1 microM Ca2+ in the pipette solution. The activation of IKvol required both Mg2+ and ATP and an increasing concentration of Mg-ATP from 0.25 through 0.5 to 0.9 mM activated IKvol increasingly faster and to a larger extent. The KCNQ1 inhibitor chromanol 293B reversibly depressed IKvol with an IC50 of 26 microM. RT-PCR detected the expression of members of the KCNQ family from KCNQ1 to KCNQ5 and of the accessory proteins KCNE1 to KCNE3 in the rat hepatocytes, but not KCNQ2 and KCNE2 in human liver. Western blotting showed KCNE3 expression in a plasma membrane-enriched fraction from rat hepatocytes. The results suggest that KCNQ1, probably with KCNE2 or KCNE3 as its accessory unit, provides a significant fraction of IKvol in rat hepatocytes.
authors.affiliation: GI Diseases Research Unit, Hotel Dieu Hospital and Queen's University, Kingston, Ontario, Canada K7L 5G2.
authors.forename: W-Z
authors.initials: WZ
authors.lastname: Lan
authors.forename: H
authors.initials: H
authors.lastname: Abbas
authors.forename: A-M
authors.initials: AM
authors.lastname: Lemay
authors.forename: M M
authors.initials: MM
authors.lastname: Briggs
authors.forename: C E
authors.initials: CE
authors.lastname: Hill
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D022781:Hepatocytes / Q000502:physiology*; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D008099:Liver / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology*; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D011188:Potassium / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000737:chemistry* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D024642:Potassium Channels, Voltage-Gated; D011188:Potassium