[Quantitative determination of 7-ketocholesterol in human fetal liver cell supernatant and lysate by high performance liquid chromatography].
7-Ketocholesterol (7-KC), which is a major oxidation product of cholesterol and is selective cytotoxic to tumour cells, was isolated from human fetal liver. A fast, sensitive method of high performance liquid chromatography for the quantitation of 7-KC has been developed and applied to the determination of 7-KC in human fetal liver cell suspension. In this procedure a mixture of 2 : 1 chloroform-methanol (V/V) was used as extraction solvent. The extract was washed with distilled water and then evaporated to dryness under N2. An adsorption liquid chromatographic system used included mu-Porasil SiO2 column, hexane : iso-propanol (91 : 9) mobile phase and UV detector at 233nm. It was found that the level of 7-KC in human fetal liver cell supernatant was higher than in liver lysate.
Zhang
Q
Q
Beijing Institute of Radiation Medicine, Beijing, 100850.
Wu
C
C
Cao
J
J
Xue
H
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005333:Fetus / Q000737:chemistry*; D006801:Humans; D007653:Ketocholesterols / Q000032:analysis*; D008099:Liver / Q000737:chemistry*
D007653:Ketocholesterols; C003001:7-ketocholesterol