Kurarinone isolated from Sophora flavescens Ait inhibited MCP-1-induced chemotaxis.
The accumulation of circulating monocytes in the arterial wall is an early in atherosclerotic plaque formation. Monocyte chemoattractant protein-1 (MCP-1) promotes the migration of monocytes and would play a role in the development of atherosclerotic lesions. Searching for inhibitors of MCP-1-induced cell migration from natural sources, we isolated one active compound through active-guided fractionations from the MeOH extracts of Sophora flavescens Ait (Leguminosae). On the basis of spectral evidence, the structure of active compound was identified as kurarinone. It inhibited the migration of THP-1 cells induced by MCP-1 with IC50 value of 19.2 microg/mL. In addition, it inhibited the binding of MCP-1 to THP-1 cells and phosphorylation of p42/44 MARK.
Lee
Seung Woong
SW
Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology, 52 Eoun-dong, Taejeon 305-333, Republic of Korea.
Lee
Hyun Sun
HS
Nam
Jung Yeon
JY
Kwon
Oh Eok
OE
Baek
Jin Ah
JA
Chang
Jong Sun
JS
Rho
Mun-Chual
MC
Kim
Young Kook
YK
D002460:Cell Line; D002464:Cell Migration Inhibition; D018932:Chemokine CCL2 / Q000037:antagonists & inhibitors* / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D029909:Sophora
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D005419:Flavonoids; D010936:Plant Extracts; C411319:kurarinone