Effect of GRP75/mthsp70/PBP74/mortalin overexpression on intracellular ATP level, mitochondrial membrane potential and ROS accumulation following glucose deprivation in PC12 cells.
Glucose regulated protein 75 (GRP75) is an important molecular chaperon belonged to the heat shock protein (HSP) family. To evaluate the effect of GRP75 overexpression on PC12 cells under glucose deprivation, cell viability and mitochondrial function of GRP75-overexpressing PC12 cells and the vector transfected control PC12 cells were monitored during glucose deprivation. Upon exposure to glucose deprivation, GRP75-overexpressing PC12 cells exhibited more moderate cell damage than control PC12 cells. Both of the two groups of cells showed a decreased ATP level following an early increase in the condition of glucose deprivation, and the mitochondrial potential were also reduced in the similar manner in the two groups of cells. Control PC12 cells showed an immediate and rapid increase in ROS accumulation after the onset of GD treatment, and this accumulation was slowed and reduced in GRP75-overexpressing PC12 cells. These findings suggested that GRP75 could inhibit the ROS accumulation, and it may be associated with the cytoprotective effect of GRP75 overexpression upon glucose deprivation.
Liu
Yan
Y
Department of Cellular and Genetic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
Liu
Wen
W
Song
Xiao-Dong
XD
Zuo
Ji
J
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D019610:Cytoprotection / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D005947:Glucose / Q000172:deficiency* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D008564:Membrane Potentials / Q000235:genetics / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; D017382:Reactive Oxygen Species; C080352:mortalin; D000255:Adenosine Triphosphate; D005947:Glucose
J Biol Chem. 1997 Jan 3;272(1):657-62
8995309
J Clin Invest. 2003 Feb;111(3):303-12
12569152
Biochem Biophys Res Commun. 1984 Sep 17;123(2):764-70
6487312
DNA Cell Biol. 1994 Dec;13(12):1213-20
7811387
Cell. 2003 Feb 21;112(4):481-90
12600312
FEBS Lett. 2000 Jun 2;474(2-3):159-64
10838077
Biochim Biophys Acta. 1998 Sep 16;1404(3):393-404
9739168
Mol Cell Biol. 2001 Oct;21(20):7097-104
11564892
J Biol Chem. 1993 Mar 25;268(9):6615-21
8454632
Trends Biochem Sci. 2001 Aug;26(8):504-10
11504627
Exp Gerontol. 2002 Oct-Nov;37(10-11):1157-64
12470827
Biochem Biophys Res Commun. 2000 Aug 18;275(1):174-9
10944461
Free Radic Biol Med. 1999 Sep;27(5-6):612-6
10490282
Mol Cell Biol. 2000 Aug;20(16):5879-87
10913171
FEBS Lett. 1996 Nov 11;397(1):7-10
8941703
J Immunol Methods. 1983 Dec 16;65(1-2):55-63
6606682
Ann N Y Acad Sci. 2000;899:349-62
10863552
Yi Chuan Xue Bao. 2000;27(8):666-71
11055118
Apoptosis. 2000 Apr;5(2):99-105
11232248
Int J Biochem Cell Biol. 2002 Sep;34(9):1059-70
12009301
Int J Radiat Biol. 2002 Mar;78(3):183-90
11869473
J Cell Mol Med. 2003 Jan-Mar;7(1):49-56
12767261
Anal Biochem. 1976 May 7;72:248-54
942051
Arch Biochem Biophys. 1993 Jun;303(2):474-82
8390225
Free Radic Biol Med. 1999 Feb;26(3-4):419-30
9895234
J Neurosci Res. 1995 Apr 15;40(6):807-19
7629893
Oncol Res. 1999;11(9):429-35
10821537