title: Modulation of mRNA stability participates in stationary-phase-specific expression of ribosome modulation factor.
abstract: The expression of ribosome modulation factor (RMF) is induced during stationary phase in Escherichia coli. RMF participates in the dimerization of 70S ribosomes to form the 100S ribosome, which is the translationally inactive form of the ribosome. To elucidate the involvement of the control of mRNA stability in growth-phase-specific rmf expression, we investigated rmf mRNA stability in stationary-phase cells and cells inoculated into fresh medium. The rmf mRNA was found to have an extremely long half-life during stationary phase, whereas destabilization of this mRNA took place after the culture was inoculated into fresh medium. RMF and 100S ribosomes disappeared from cells 1 min after inoculation. In addition to control by ppGpp-dependent transcription, these results indicate that the modulation of rmf mRNA stability is also involved in the regulation of growth-phase-specific rmf expression. Unexpectedly, the postinoculation degradation of rmf mRNA was suppressed by the addition of rifampin, suggesting that de novo RNA synthesis is necessary for degradation. This degradation was also suppressed in both a poly(A) polymerase-deficient and an rne-131 mutant strain. We cloned and sequenced the 3'-proximal regions of rmf mRNAs and found that most of these 3' ends terminated at the rho-independent terminator with the addition of a one- to five-A oligo(A) tail in either stationary-phase or inoculated cells. No difference was observed in the length of the poly(A) tail between stationary-phase and inoculated cells. These results suggest that a certain postinoculation-specific regulatory factor participates in the destabilization of rmf mRNA and is dependent on polyadenylation.
authors.affiliation: Department of Molecular Biology, School of Health Sciences, Kyorin University, Hachioji, Tokyo, Japan.
authors.forename: Toshiko
authors.initials: T
authors.lastname: Aiso
authors.forename: Hideji
authors.initials: H
authors.lastname: Yoshida
authors.forename: Akira
authors.initials: A
authors.lastname: Wada
authors.forename: Reiko
authors.initials: R
authors.lastname: Ohki
mesh_terms: D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D009690:Nucleic Acid Conformation; D026723:Polyadenylation / Q000502:physiology; D020871:RNA Stability / Q000502:physiology*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D012270:Ribosomes / Q000502:physiology; D012293:Rifampin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D004791:Enzyme Inhibitors; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C063545:ribosome modulation factor, E coli; D012293:Rifampin
references.citation: J Mol Biol. 1988 Jul 5;202(1):45-58
references.pmid: 3050121
references.citation: EMBO J. 1993 Feb;12(2):625-30
references.pmid: 8440252
references.citation: Proc Natl Acad Sci U S A. 1990 Apr;87(7):2657-61
references.pmid: 2181444
references.citation: J Bacteriol. 1993 Dec;175(24):7982-9
references.pmid: 8253685
references.citation: Annu Rev Genet. 1999;33:193-227
references.pmid: 10690408
references.citation: Biochem Biophys Res Commun. 1997 Oct 9;239(1):46-50
references.pmid: 9345267
references.citation: J Biol Chem. 1956 Jan;218(1):97-106
references.pmid: 13278318
references.citation: J Bacteriol. 1998 Mar;180(6):1389-95
references.pmid: 9515904
references.citation: J Bacteriol. 1988 Oct;170(10):4625-33
references.pmid: 2459106
references.citation: Genes Dev. 1998 Sep 1;12(17):2770-81
references.pmid: 9732274
references.citation: Nature. 1984 Nov 1-7;312(5989):75-7
references.pmid: 6387508
references.citation: J Biol Chem. 1981 Nov 25;256(22):11905-10
references.pmid: 6271763
references.citation: Genes Cells. 1998 Apr;3(4):203-8
references.pmid: 9663655
references.citation: J Bacteriol. 1993 Jan;175(1):229-39
references.pmid: 8416898
references.citation: Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1807-11
references.pmid: 7534403
references.citation: J Biochem. 2002 Dec;132(6):983-9
references.pmid: 12473202
references.citation: J Bacteriol. 1997 Dec;179(23):7476-87
references.pmid: 9393714
references.citation: J Biol Chem. 1999 Nov 12;274(46):33105-13
references.pmid: 10551881
references.citation: Nucleic Acids Res. 2003 Jul 15;31(14):4017-23
references.pmid: 12853618
references.citation: J Bacteriol. 1995 Dec;177(23):6832-5
references.pmid: 7592475
references.citation: Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11637-42
references.pmid: 9751718
references.citation: J Bacteriol. 1996 Jul;178(13):3917-25
references.pmid: 8682798
references.citation: Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1501-5
references.pmid: 10677490
references.citation: Genes Cells. 2004 Apr;9(4):271-8
references.pmid: 15066119
references.citation: Biochem Biophys Res Commun. 1999 Oct 22;264(2):525-9
references.pmid: 10529396
references.citation: J Bacteriol. 1991 Aug;173(15):4578-86
references.pmid: 1713205
references.citation: Genes Cells. 2001 Aug;6(8):665-76
references.pmid: 11532026
references.citation: Genes Cells. 2003 Feb;8(2):179-87
references.pmid: 12581159
references.citation: Mol Microbiol. 2003 Jan;47(1):75-88
references.pmid: 12492855
references.citation: Biochem Biophys Res Commun. 1995 Sep 14;214(2):410-7
references.pmid: 7677746
references.citation: Cell. 2002 Nov 27;111(5):611-3
references.pmid: 12464173
references.citation: Proc Natl Acad Sci U S A. 1997 May 27;94(11):5628-33
references.pmid: 9159123
references.citation: J Mol Biol. 1973 Apr 5;75(2):281-94
references.pmid: 4580677
references.citation: EMBO Rep. 2003 Feb;4(2):172-7
references.pmid: 12612607
references.citation: Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14118-23
references.pmid: 9826663
references.citation: Prog Nucleic Acid Res Mol Biol. 1996;52:153-216
references.pmid: 8821261
references.citation: Genes Genet Syst. 2002 Aug;77(4):219-25
references.pmid: 12419894
references.citation: J Biochem. 1981 Aug;90(2):449-61
references.pmid: 6170632
references.citation: FEBS Lett. 1969 Oct 21;5(2):104-106
references.pmid: 11947251