Expression pattern of intracellular leukocyte-associated proteins in primary mediastinal B cell lymphoma.
Two microarray studies of mediastinal B cell lymphoma have shown that this disease has a distinct gene expression profile, and also that this is closest to the pattern seen in classical Hodgkin's disease. We reported previously an immunohistologic study in which the loss of intracellular B cell-associated signaling molecules in Reed-Sternberg cells was demonstrated, and in this study we have investigated the expression of the same components in more than 60 mediastinal B cell lymphomas. We report that these signaling molecules are frequently present, and in particular that Syk, BLNK and PLC-gamma2 (absent from Reed-Sternberg cells) are present in the majority of mediastinal B cell lymphomas. The overall pattern of B cell signaling molecules in this disease is therefore closer to that of diffuse large B cell lymphoma than to Hodgkin's disease, and is consistent with a common cell of origin as an explanation of the similar gene expression profiles.
Marafioti
T
T
Leukaemia Research Fund Immunodiagnostics Unit, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, UK. teresa.marafioti@ndcls.ox.ac.uk
Pozzobon
M
M
Hansmann
M-L
ML
Gaulard
P
P
Barth
T F
TF
Copie-Bergman
C
C
Roberton
H
H
Ventura
R
R
Martín-Subero
J I
JI
Gascoyne
R D
RD
Pileri
S A
SA
Siebert
R
R
Hsi
E D
ED
Natkunam
Y
Y
Möller
P
P
Mason
D Y
DY
D048868:Adaptor Proteins, Signal Transducing; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis; D004792:Enzyme Precursors / Q000032:analysis / Q000096:biosynthesis*; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008479:Mediastinal Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis; D051966:Phospholipase C gamma; D010750:Phosphoproteins / Q000032:analysis / Q000096:biosynthesis*; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis*; D015398:Signal Transduction; D000072377:Syk Kinase; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis; D010738:Type C Phospholipases / Q000032:analysis / Q000096:biosynthesis*; D019061:src-Family Kinases / Q000032:analysis / Q000096:biosynthesis
D048868:Adaptor Proteins, Signal Transducing; C113939:B cell linker protein; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases; D010738:Type C Phospholipases; D051966:Phospholipase C gamma