Microelectrochemical approach to induce local cell adhesion and growth on substrates.
The nature of an albumin-coated substrate that blocks protein adsorption and cell adhesion was rapidly switched to cell-adhesive by exposure to an oxidizing agent such as HBrO. This finding has enabled cellular pattern drawing even on a single-cell level by closely scanning a microelectrode above the substrate and electrochemically producing the agent at the tip of the electrode. The present microelectrochemical cell patterning is applicable even for a previously cell-patterned substrate and for a grooved substrate. These unique technical features will have impacts on a variety of cell-based studies that require the analysis of heterotypic cell-cell interactions and cellular arrangement on an uneven surface such as semiconductor devices.
Kaji
Hirokazu
H
Kanada
Masamitsu
M
Oyamatsu
Daisuke
D
Matsue
Tomokazu
T
Nishizawa
Matsuhiko
M
D001959:Bromates / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D004563:Electrochemistry; D006367:HeLa Cells; D006801:Humans; D008839:Microelectrodes; D012666:Semiconductors; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000187:drug effects*; D013499:Surface Properties
D001959:Bromates; D012710:Serum Albumin, Bovine; C027664:hypobromous acid