Detection of the enterotoxins A, B, and C genes in Staphylococcus aureus from goat and bovine mastitis in Brazilian dairy herds.
To determine the distribution of genes that encode enterotoxins A, B and C, 36 strains of Staphylococcus aureus isolated from goat mastitis and 64 isolated from bovine mastitis were analyzed by Multiplex PCR. Of the total strains studied, 37 (37%) were detected to have some of the SEs genes. From the bovine mastitis strains, 4 (6.3%) co-amplified the sea and seb genes and 2 (3.1%) were positive for the sec gene. From the goat mastitis strains, 31 (86%) tested positive to the Multiplex, and the sec gene was detected in all of them. The production of SE was detected in all strains harboring the corresponding gene. The results demonstrated that S. aureus isolated from goat mastitis had a higher enterotoxigenic potential than those isolated from bovine mastitis. Additionally, the presence of the sec gene in the majority of goat mastitis strains suggests a possible involvement of SEC in goat mastitis pathogenesis.
da Silva
Elizabete Rodrigues
ER
Laboratory of Mastitis Control, Veterinary School - Federal University of Minas Gerais/UFMG, Av. Antônio Carlos, 6627, Campus Pampulha, CEP 30161-970, Belo Horizonte, Minas Gerais, Brazil. beteers@hotmail.com
do Carmo
Luiz Simeão
LS
da Silva
Nivaldo
N
D000818:Animals; D001938:Brazil; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005798:Genes, Bacterial; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D008414:Mastitis, Bovine / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D004269:DNA, Bacterial; D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; C034959:enterotoxin C, staphylococcal