Gradient ion-pair chromatographic method for the determination of iron N,N'-ethylenediamine-di-(2-hydroxy-5-sulfophenylacetate) by high performance liquid chromatography-atmospheric pressure ionization electrospray mass spectrometry.
The most effective remedy for iron deficiency is the use of synthetic iron chelates, specifically chelates derived from polyaminecarboxylic acids as EDDHSA (N,N'-ethylenediamine-di-(2-hidroxy-5-sulfophenylacetic) acid). A gradient ion-pair chromatographic method was developed to quantify the total amount of chelated iron in EDDHSA/Fe3+ fertilizers. Two mobile phases were used containing, respectively, 35 and 75% acetonitrile in a 0.005 M tetrabutylammonium hydroxide aqueous solution at pH 6.0. The stationary phase was a reverse phase C-18 column (150mm x 3.9mm i.d., dp = 5 microm). Two chromatographic peaks appeared and were identified by Electrospray Mass Spectrometry. The first peak corresponds to the monomer of EDDHSA/Fe3+ and the second peak has been assigned to condensation molecules. Quality parameters indicate that the method is suitable for the quantification of iron chelate by EDDHSA fertilizers.
García-Marco
Sonia
S
Sección de Química Agrícola, Facultad de Ciencias C-VII, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Cremonini
Mauro A
MA
Esteban
Pedro
P
Yunta
Felipe
F
Hernández-Apaolaza
Lourdes
L
Placucci
Giuseppe
G
Lucena
Juan J
JJ
D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010648:Phenylacetates / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
C501683:N,N'-ethylenediamine-di-(2-hidroxy-5-sulfophenylacetic) acid; D010648:Phenylacetates