Removal of contaminating DNA from commercial nucleic acid extraction kit reagents.
Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained.
Mohammadi
Tamimount
T
Sanquin Blood Bank North West Region, Amsterdam, The Netherlands.
Reesink
Henk W
HW
Vandenbroucke-Grauls
Christina M J E
CM
Savelkoul
Paul H M
PH
D001792:Blood Platelets / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005189:False Positive Reactions; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic