The structure of the O-polysaccharide from the lipopolysaccharide of Providencia stuartii O57 containing an amide of D-galacturonic acid with L-alanine.
The O-polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O57:H29. Studies by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC, and HMBC experiments, showed that the polysaccharide contains an amide of D-galacturonic acid with L-alanine and has the following pentasaccharide repeating unit: [formula: see text]
Kocharova
Nina A
NA
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, RUS-119991 Moscow, Russian Federation.
Ovchinnikova
Olga G
OG
Bushmarinov
Ivan S
IS
Toukach
Filip V
FV
Torzewska
Agnieszka
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D000409:Alanine / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006603:Hexuronic Acids / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D011532:Providencia / Q000737:chemistry* / Q000276:immunology
D006603:Hexuronic Acids; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; C007819:galacturonic acid; D000409:Alanine