title: Separation of aceclofenac and diclofenac in human plasma by free zone capillary electrophoresis using N-methyl-D-glucamine as an effective electrolyte additive.
abstract: Aceclofenac (A) and diclofenac (D) are effective non-steroidal anti-inflammatory drugs (NSAIDs) derived from the phenylacetic acid with pronounced antirheumatic, anti-inflammatory, analgesic and antipyretic properties. Our work proposes a new, fast-free zone capillary electrophoresis method for the simultaneous determination of aceclofenac and diclofenac in human plasma. The effect of increasing concentrations of N-methyl-D-glucamine organic base on borate run buffer was investigated. A good separation was achieved using a 40 cm x 75 microm uncoated silica capillary, 300 mmol/l sodium borate buffer, 200 mmol/l N-methyl-D-glucamine, pH 8.9, in about 3 min. Moreover, the plasma sample pre-treatment procedure was examined: acidic precipitants such as trichloroacetic acid (TCA), metaphosphoric acid (MPA), perchloric acid (PCA) or 5-sulphosalicylic acid (SSA) cause a total loss of analytes while acetonitrile allows a recovery of 97-98% of both compounds. Its simplicity and rapidity and the low analysis costs demonstrate that our method is a reliable and efficient mean for the comprehensive determination of aceclofenac and diclofenac in human plasma when pharmacokinetics studies are required.
authors.affiliation: Clinical Biochemistry, University of Sassari, Viale San Pietro 43/B, I-07100 Sassari, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sotgia
authors.forename: Emanuela
authors.initials: E
authors.lastname: Porqueddu
authors.forename: Paolo
authors.initials: P
authors.lastname: Enrico
authors.forename: Luca
authors.initials: L
authors.lastname: Deiana
mesh_terms: D000328:Adult; D004008:Diclofenac / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D005942:Gluconates / Q000737:chemistry*; D006801:Humans; D008297:Male; D008536:Meglumine / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D004573:Electrolytes; D005942:Gluconates; C051643:N-methylglucamine gluconate; D004008:Diclofenac; D008536:Meglumine; C056498:aceclofenac