The Rok protein of Bacillus subtilis represses genes for cell surface and extracellular functions.
Rok is a repressor of the transcriptional activator ComK and is therefore an important regulator of competence in Bacillus subtilis (T. T. Hoa, P. Tortosa, M. Albano, and D. Dubnau, Mol. Microbiol. 43:15-26, 2002). To address the wider role of Rok in the physiology of B. subtilis, we have used a combination of transcriptional profiling, gel shift experiments, and the analysis of lacZ fusions. We demonstrate that Rok is a repressor of a family of genes that specify membrane-localized and secreted proteins, including a number of genes that encode products with antibiotic activity. We present evidence for the recent introduction of rok into the B. subtilis-Bacillus licheniformis-Bacilllus amyloliquefaciens group by horizontal transmission.
Albano
Mark
M
Public Health Research Institute, Newark, NJ 07103, USA.
Smits
Wiep Klaas
WK
Ho
Linh T Y
LT
Kraigher
Barbara
B
Mandic-Mulec
Ines
I
Kuipers
Oscar P
OP
Dubnau
David
D
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D008565:Membrane Proteins / Q000235:genetics; D010455:Peptides / Q000235:genetics; D010456:Peptides, Cyclic; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D001430:Bacteriocins; D008565:Membrane Proteins; D010455:Peptides; D010456:Peptides, Cyclic; D012097:Repressor Proteins; D014157:Transcription Factors; C087449:comK protein, Bacillus subtilis; C047828:sboA protein, Bacillus subtilis
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