Optimum dose of LH-RH analogue Fertirelin Acetate for the induction of superovulation in mice.
The optimum dose for establishing superovulation in mice of Fertirelin Acetate (FA), an LH-RH analogue, was examined. Mice were subcutaneously injected with 5 IU of hCG at 17:00 (Day 0), and with various doses of FA (0.001 to 1.0 microg) five times at 4 h intervals on and after 22:00 on Day 0. To induce ovulation, 5 IU of hCG was again injected subcutaneously at 17:00 on Day 2. In the groups administered with doses ranging from 0.01 to 0.5 microg of FA, the number of ovulated eggs was significantly (p<0.05) larger than in the control group (12.9 +/- 5.9). The greatest number of ovulated eggs (22.6 +/- 7.3) was obtained in the group administered with 0.025 microg of FA. The results indicate that the effective dose of LH-RH analogue, FA, is between 0.1 and 0.5 microg for superovulation induction in mice.
Nariai
Koichi
K
Laboratory Animal Facilities, Research Center for Medical Sciences, The Jikei University School of Medicine, Kashiwa, Chiba, Japan.
Ishinazaka
Tsuyoshi
T
Suzuki
Kazuyosi
K
Uchiyama
Hiroshi
H
Sato
Kahei
K
Asano
Ryuji
R
Tsumagari
Shigehisa
S
Yukawa
Masayoshi
M
Kanayama
Kiichi
K
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D010062:Ovulation Induction / Q000379:methods*; D013480:Superovulation / Q000187:drug effects
D007987:Gonadotropin-Releasing Hormone; C009936:fertirelin