title: Singlet oxygen quenching activity of human serum.
abstract: Singlet oxygen is regarded as contributing to the pathogenesis of various diseases including light-induced skin disorders and inflammatory response. In this study, the correlation between singlet oxygen quenching activity (SOQA) of human serum and blood biochemistry or life-style was evaluated. Healthy volunteers were recruited and carried out a measurement of SOQA by using electron paramagnetic resonance (EPR) and a questionnaire survey about a smoking. It was demonstrated that major quenchers of singlet oxygen in serum are proteins, and small molecular anti-oxidants relatively play a minor role. SOQA of whole sera showed no correlation with protein concentration, but positively correlated with SOQA of small molecular fraction. In vitro studies demonstrated that the decrease of sulfhydryl groups by NO or superoxide significantly attenuated SOQA of albumin. Together, these results may imply that the underlying oxidative condition in each individual influences both small molecular antioxidant states and the sulfhydryl content of serum proteins. SOQA of sera from women with a smoking history was significantly lower compared to non-smoking women, suggesting that the smoking habit impaired the defense mechanism against singlet oxygen.
authors.affiliation: Central Research Laboratory, FANCL Corporation, Kanagawa, Yokohama, Japan. kon@fancl.co.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kon
authors.forename: Toru
authors.initials: T
authors.lastname: Tanigawa
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Hayamizu
authors.forename: Manzhen
authors.initials: M
authors.lastname: Shen
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tsuji
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000032:analysis / Q000502:physiology*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D044967:Serum; D012709:Serum Albumin / Q000032:analysis; D026082:Singlet Oxygen / Q000097:blood* / Q000502:physiology*; D012907:Smoking / Q000097:blood; D013438:Sulfhydryl Compounds / Q000097:blood; D005719:gamma-Globulins / Q000032:analysis
chemical_list: D001798:Blood Proteins; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D005719:gamma-Globulins; D026082:Singlet Oxygen