Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing.
Amino acid sequence analyses indicate that the Soilborne wheat mosaic virus (SBWMV) 19K protein is a cysteine-rich protein (CRP) and shares sequence homology with CRPs derived from furo-, hordei-, peclu- and tobraviruses. Since the hordei- and pecluvirus CRPs were shown to be pathogenesis factors and/or suppressors of RNA silencing, experiments were conducted to determine if the SBWMV 19K CRP has similar activities. The SBWMV 19K CRP was introduced into the Potato virus X (PVX) viral vector and inoculated to tobacco plants. The SBWMV 19K CRP aggravated PVX-induced symptoms and restored green fluorescent protein (GFP) expression to GFP silenced tissues. These observations indicate that the SBWMV 19K CRP is a pathogenicity determinant and a suppressor of RNA silencing.
Te
Jeannie
J
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA. te@okstate.edu
Melcher
Ulrich
U
Howard
Amanda
A
Verchot-Lubicz
Jeanmarie
J
D000595:Amino Acid Sequence; D003545:Cysteine / Q000032:analysis*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000378:metabolism*; D017863:Potexvirus / Q000472:pathogenicity; D034622:RNA Interference / Q000502:physiology*; D014026:Nicotiana / Q000821:virology; D014908:Triticum / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*
D014764:Viral Proteins; D003545:Cysteine
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