title: Characterisation of myofibroblasts in fibrovascular tissues of primary and recurrent pterygia.
abstract: OBJECTIVE
  To determine the presence and origin of myofibroblasts in pterygia.
  
  
  METHODS
  86 specimens including head, body, and fibrovascular tissue from 52 primary and 34 recurrent pterygia and five exenterated eyes without pterygia were searched for the origin of myofibroblasts. All tissues were subjected to haematoxylin and eosin staining, immunohistochemistry using antibodies against alpha smooth muscle actin (alpha-SMA), desmin, vimentin, and caldesmon, and transmission electron microscopy (TEM). The phenotype of fibroblasts subcultured in a serum free medium from pterygium fibrovascular tissues was characterised by the above antibodies. Bundles of dense fibrous tissues were noted in 86% of the fibrovascular tissue specimens evaluated. Cells within these bundles were characterised as myofibroblasts based on positive staining to alpha-SMA, but negative to desmin and caldesmon, markers for smooth muscle cells. Interestingly, positive alpha-SMA staining was also found in the periorbital fibroadipose tissue posterior to Tenon's capsule near the nasal conjunctiva in all exenterated specimens. All first passage fibroblasts expressed vimentin, some were positive to alpha-SMA, but all were negative to desmin or caldesmon. Cells in pterygium fibrovascular tissues showed ultrastructural features of intracytoplasmic bundles of microfilaments, consistent with myofibroblastic differentiation.
  
  
  CONCLUSIONS
  These studies collectively demonstrate the presence of contractile myofibroblasts bundle in pterygia and in the periorbital fibroadipose tissue posterior to Tenon's capsule of exenterated eyes without pterygium.
authors.affiliation: Ocular Surface Center, 7000 SW 97 Avenue, Miami, FL 33173, USA.
authors.forename: A
authors.initials: A
authors.lastname: Touhami
authors.forename: M A
authors.initials: MA
authors.lastname: Di Pascuale
authors.forename: T
authors.initials: T
authors.lastname: Kawatika
authors.forename: M
authors.initials: M
authors.lastname: Del Valle
authors.forename: R H
authors.initials: RH
authors.lastname: Rosa
authors.forename: S
authors.initials: S
authors.lastname: Dubovy
authors.forename: S C G
authors.initials: SC
authors.lastname: Tseng
mesh_terms: D000199:Actins / Q000032:analysis; D000273:Adipose Tissue / Q000473:pathology; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D003893:Desmin / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D005355:Fibrosis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009132:Muscles / Q000473:pathology*; D011625:Pterygium / Q000473:pathology*; D012008:Recurrence; D014746:Vimentin / Q000032:analysis
chemical_list: D000199:Actins; D015415:Biomarkers; D002148:Calmodulin-Binding Proteins; D003893:Desmin; D014746:Vimentin
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