Transcriptional profiling of human herpesvirus type B (HHV-6B) in an adult T cell leukemia cell line as in vitro model for persistent infection.
Human herpesvirus 6 (HHV-6), which is present in more than 90% of the human, is known to cause infectious diseases in immuno-compromised patients, e.g., transplant patients. To clarify the possible role of the pattern of expression of HHV-6 genes in various types of HHV-6B infection, we sought to determine whether or not viral DNA microarray could be used for detailed characterization of viral transcription using a HHV-6B DNA microarray that contains 97 known open reading frames of HHV-6B. A subset of genes are preferentially expressed in persistent infection: U16 (IE-B, transactivator, US22 gene family), U18 (IE-B, homolog to HCMV IE glycoprotein), U20 (glycoprotein), U27 (DNA polymerase processivity transactivator), U82 (gL, gH accessory protein), U83 (chemokine), U85 (OX-2 homology, glycoprotein), U90 (IE-A), and U94 (transactivator), respectively. Although the function of each HHV-6B is not fully understood, our study suggests that comprehensive analysis of HHV-6B transcription is useful not only to clarify the pathogenesis of the virus but also to develop new strategies for anti-viral drugs.
Ohyashiki
Junko H
JH
Intractable Immune System Disease Research Center, Tokyo Medical University, Tokyo, Japan. junko@hh.iij4u.or.jp
Takaku
Tomoiku
T
Ojima
Tomoko
T
Abe
Kenji
K
Yamamoto
Kohtaro
K
Zhang
Yu
Y
Ohyashiki
Kazuma
K
D045744:Cell Line, Tumor; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry; D016366:Open Reading Frames; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004279:DNA, Viral; D009841:Oligonucleotides; D012333:RNA, Messenger; D012313:RNA; D004259:DNA-Directed DNA Polymerase