Cloning of the Atlantic salmon (Salmo salar) IL-1 receptor associated protein.
In mammals, the pro-inflammatory cytokine interleukin-1 signals through a receptor complex containing a type I interleukin-1 receptor (IL-1RI) and a receptor associated protein (IL-1RAcP). Previously, we have described a cDNA from Atlantic salmon encoding a molecule with homology to the mammalian IL-RI. This molecule was named IL-1 receptor like protein (IL-1RLP) in the absence of functional data to support its proposed role as the salmon IL-1RI. Here, we describe the cloning and characterisation of a cDNA encoding salmon IL-1RAcP. Like other members of the IL-1R family, the salmon IL-1RAcP encodes three extracellular immunoglobulin-like domains and a cytoplasmic Toll/Interleukin-1 receptor (TIR) domain involved in signalling. Specific binding of salmon IL-1RAcP to IL-1RLP was shown by co-immunoprecipitation studies.
Stansberg
Christine
C
Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, 5008 Bergen, Norway.
Subramaniam
Sumathi
S
Collet
Bertrand
B
Secombes
Christopher J
CJ
Cunningham
Charles
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers; D015723:Gene Library; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D020317:Salmo salar / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000276:immunology
D017931:DNA Primers; D008565:Membrane Proteins; D017472:Receptors, Interleukin-1