Early effects of transplatin on oncogene activation in vivo.
The aim of the study was to investigate the early effect of Transplatin (the stereo-isomer of Cisplatin) on oncogenes in inbred CBA/Ca mice. Cisplatin is commonly used for the treatment of squamous cell carcinomas of the head and neck. Cisplatin has a strong oncogene activation effect compared to the structural analogue Transplatin. Body weight equivalent amounts of a human dose of Transplatin were administered intra-peritoneally to 6- to 8-week-old, inbred, female CBA/Ca mice. Twenty-four, 48 and 72 hours after the treatment, RNA was isolated from the target organs and the expressions of c-myc, Ha-ras and p53 genes were examined. Investigation of early changes showed no significant overexpression compared to Cisplatin, which had a significant effect on oncogene expression in the "short-term" in vivo test system.
Németh
A
A
Department of Public Health, Faculty of Medicine, University of Pécs, Hungary. arpadnemeth@freemail.hu
Nádasi
E
E
Beró
A
A
Olasz
L
L
Ember
A
A
Kvarda
A
A
Bujdosó
L
L
Arany
I
I
Csejtei
A
A
Faluhelyi
Zs
Z
Ember
I
I
D000818:Animals; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D016259:Genes, myc / Q000187:drug effects* / Q000235:genetics; D016158:Genes, p53 / Q000187:drug effects* / Q000235:genetics; D011905:Genes, ras / Q000187:drug effects* / Q000235:genetics; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000502:physiology; D008168:Lung / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000187:drug effects / Q000502:physiology
C034697:transplatin; D002945:Cisplatin