Immunogenicity of major cell surface protein(s) of Brucella melitensis Rev 1.
Brucella melitensis Rev 1 organisms were salt-extracted and the cell surface proteins (BCSPs) were found to be mainly 39-42 kDa (group 2 porin proteins) in addition to 31.6, 32.5, 58.5 and 14.7 kDa proteins. DEAE-Sephadex anion-exchange column chromatography of BCSPs yielded fraction 1, which contained one major protein (39.8-42.0 kDa) and a minor protein (31.6 kDa). All these proteins were found to be immunogenic by Western blotting. Fraction 1 along with monophosphoryl lipid A and trehalose dicorynomycolate adjuvants as well as BCSPs alone induced significant (p < or = 0.05) protection in BALB/c mice. Both these immunizing agents produced almost equivalent protection to live B. melitensis Rev 1 vaccine at 15 and 30 days post challenge. Lymphocyte stimulation test as well as delayed-type hypersensitivity reaction revealed that both these preparations induced cell-mediated immune response. These preparations also induced humoral immune response as indicated by indirect ELISA. Neither of the immune responses was significantly less (p < or = 0.05) than that with live B. melitensis Rev 1 vaccine, except that their duration was short.
Mahajan
N K
NK
Department of Veterinary Epidemiology and Preventive Medicine, CCS Haryana Agricultural University, Hisar-125 004, India. mahajannk@hau.nic.in
Kulshreshtha
R C
RC
Malik
G
G
Dahiya
J P
JP
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D017347:Brucella melitensis / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control / Q000662:veterinary; D002852:Chromatography, Ion Exchange / Q000662:veterinary; D003637:DEAE-Dextran; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C471349:Rev.1 vaccine, Brucella melitensis; D003637:DEAE-Dextran