Characterization of Rhodococcus wratislaviensis strain J3 that degrades 4-nitrocatechol and other nitroaromatic compounds.
The bacterial strain J3 was isolated from soil by selective enrichment on mineral medium containing 4-nitrocatechol as the sole carbon and energy source. This strain was identified as Rhodococcus wratislaviensis on the basis of morphology, biochemical, physiological and chemotaxonomic characterization and complete sequencing of the 16S rDNA gene. The isolated bacterium could utilize 4-nitrocatechol, 3-nitrophenol and 5-nitroguaiacol as sole carbon and energy sources. Stoichiometric release of nitrites was measured during degradation of 4-nitrocatechol both in growing cultures and for stationary phase cells. The J3 strain was unable to degrade 4-nitroguaiacol, 2-nitrophenol, 4-nitrophenol, 2,4-dinitrobenzoic acid, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-difluoro-6-nitrophenol. The J3 strain is deposited in the Czech Collection of Microorganisms as CCM 4930.
Navrátilová
Jitka
J
Department of Microbiology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic. jitka_n@email.cz
Tvrzová
Ludmila
L
Durnová
Eva
E
Spröer
Cathrin
C
Sedlácek
Ivo
I
Neca
Jirí
J
Nemec
Miroslav
M
D015373:Bacterial Typing Techniques; D002396:Catechols / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis / Q000302:isolation & purification; D009573:Nitrites / Q000032:analysis / Q000378:metabolism; D009574:Nitro Compounds / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D002396:Catechols; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009171:Mycolic Acids; D009573:Nitrites; D009574:Nitro Compounds; D009596:Nitrophenols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C001833:4-nitrocatechol; C049017:3-nitrophenol