title: [Pseudolaric acid B induces human melanoma A375-S2 cell apoptosis in vitro].
abstract: OBJECTIVE
  To study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells.
  
  
  METHODS
  MTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused.
  
  
  RESULTS
  Pseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased.
  
  
  CONCLUSIONS
  The major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Gong
authors.forename: Min-Wei
authors.initials: MW
authors.lastname: Wang
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tashiro
authors.forename: Satoshi
authors.initials: S
authors.lastname: Onodera
authors.forename: Takashil
authors.initials: T
authors.lastname: Ikejima
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D028201:Pinaceae / Q000737:chemistry; D011506:Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
chemical_list: D051017:Apoptosis Regulatory Proteins; C494826:BAX protein, human; C494811:BCL2L1 protein, human; D004224:Diterpenes; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C109664:caspase-activated DNase inhibitor; C058391:pseudolaric acid B