Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex.
In the present study the developmental profile of ATP-hydrolyzing activity promoted by NTPDase 1, its kinetic properties and the enzyme protein abundance associated with synaptic plasma membrane from rat cerebral cortex were characterized. NTPDase 1 activity increased from birth to day 30; afterwards it decreased and remained unchanged from adulthood (90 days) to senescence (365 days). Kinetic analysis revealed that enzyme exhibited the highest specific activity at day 30 and highest apparent affinity for ATP at day 365; however, V(max)/K(m) values remained unchanged for each age studied. Immunoblot analysis demonstrated that relative abundance of NTPDase 1 is highest at day 15 during ontogeny. The discrepancy between maximum enzyme activity and maximum enzyme protein abundance indicates that NTPDase 1 may have an additional role during development.
Nedeljkovic
N
N
Department of General Physiology and Biophysics, Faculty of Biology, University of Belgrade, Studentski trg 3, 11001 Belgrade, Yugoslavia. nnedel@bf.bio.bg.ac.yu
Banjac
A
A
Horvat
A
A
Stojiljkovic
M
M
Nikezic
G
G
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase; D015153:Blotting, Western / Q000379:methods; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013570:Synaptic Membranes / Q000201:enzymology*
D015703:Antigens, CD; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D001081:Apyrase; C067869:CD39 antigen