Negative correlation between poly-ADP-ribosylation of spleen cell histone proteins and initial duration of dimethylnitrosamine exposure to mice in vivo measured by Western blot immunoprobe assay: a possible biomarker for cancer detection.
Improved cancer detection involving suitable biomarkers with easy applicability is a challenge to our fight against cancer. Poly-ADP-ribosylation (PAR) of proteins is a likely candidate biomarker for this purpose because it meets the criterion well. This report is a step towards testing suitability of PAR as a biomarker for cancer detection. Swiss albino mice were exposed to hepatocarcinogen, dimethylnitrosamine (DMN), at a chronic dose, which is known to induce carcinogenesis in liver. PAR was monitored by a Western blot immunoprobe assay in spleen, a lymphoid organ, to find a correlation between PAR of spleen histone proteins and duration of DMN exposure. A negative, non-linear correlation was found for most histone proteins. The inhibition of PAR of histones was significant from 4 weeks onwards until the end of the observation. The inhibition was potentiated when 3-aminobenzamide was simultaneously administered. The results open up the possibility of PAR of cellular proteins being used as biomarker for cancer detection.
Devi
Brahmacharimayum J
BJ
Radiation and Molecular Biology Unit, Department of Biochemistry, North Eastern Hill University, Shillong 793022, India.
Schneeweiss
Frank H A
FH
Sharan
Rajeshwar N
RN
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D004128:Dimethylnitrosamine / Q000008:administration & dosage / Q000633:toxicity; D006657:Histones / Q000032:analysis; D007118:Immunoassay / Q000379:methods; D008113:Liver Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental; D011064:Poly Adenosine Diphosphate Ribose / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013154:Spleen / Q000737:chemistry / Q000166:cytology*
D014408:Biomarkers, Tumor; D006657:Histones; D011506:Proteins; C024857:poly(ADP)-ribosylated proteins; D011064:Poly Adenosine Diphosphate Ribose; D004128:Dimethylnitrosamine