Immunosensor for rapid detection of gibberellin acid in the rice grain.
A rapid, selective, sensitive, accurate, and inexpensive immunosensor for gibberellin acid detection was designed by coupling immunoassay with the square wave anodic stripping voltammetry (SWASV) technique involving copper ion labeled antigen in the competitive immunoreaction. The response signal expressed as the percentage of current reduction CR % (y) is linearly related to the concentration of GA (x) in the 1 microg/mL to approximately 150 microg/mL range with a regression equation of the form y = 0.44x + 15.59 and a correlation coefficient of 0.99. The results of the immunosensor assay were compared with those obtained by HPLC and ELISA, which show a satisfactory agreement. The immunosensor was used to determine the GA content in the hybrid rice grain samples taken in the growing period.
Li
Jin
J
State Key laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University, Changsha 410082, People's Republic of China.
Xiao
Lang-Tao
LT
Zeng
Guang-Ming
GM
Huang
Guo-He
GH
Shen
Guo-Li
GL
Yu
Ru-Qin
RQ
D000941:Antigens; D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D003300:Copper; D004797:Enzyme-Linked Immunosorbent Assay; D005875:Gibberellins / Q000032:analysis*; D007106:Immune Sera; D007118:Immunoassay / Q000379:methods*; D012275:Oryza / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D000941:Antigens; D005875:Gibberellins; D007106:Immune Sera; D003300:Copper; C007842:gibberellic acid