title: Miniaturization of the structure elucidation of novel natural products--two trace antibacterial acylated caprylic alcohol glycosides from Arctostaphylos pumila.
abstract: High-throughput isolation, purification and analysis methods applied to natural products libraries from plants gave rise to the discovery of two novel acylated caprylic alcohol glycosides (1, 2) produced by Arctostaphylos pumila. The NMR spectra were acquired using the CapNMR probe and performed on mass-limited samples, which enabled us to elucidate the structures of 2,6-diacetyl-3,4-diisobutyl-1- O-octylglucopyranoside (1, 200 microg) and 2,6-diacetyl-3,4-dimethylbutyl-1- O-octylglucopyranosid (2, 70 microg). Compounds 1 and 2 exhibited antibacterial activity against Gram-positive methicillin-resistant Staphylococcus aureus with an MIC of 128 microg/mL and 64 microg/mL, respectively.
authors.affiliation: Lead Discovery and Rapid Structure Elucidation Group, Sequoia Sciences, Inc., San Diego, CA 92121, USA. jhu@sequoiasciences.com
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Hu
authors.forename: Hye-Dong
authors.initials: HD
authors.lastname: Yoo
authors.forename: Caroline T
authors.initials: CT
authors.lastname: Williams
authors.forename: Eliane
authors.initials: E
authors.lastname: Garo
authors.forename: Peadar A
authors.initials: PA
authors.lastname: Cremin
authors.forename: Lu
authors.initials: L
authors.lastname: Zeng
authors.forename: Helene C
authors.initials: HC
authors.lastname: Vervoort
authors.forename: Chris M
authors.initials: CM
authors.lastname: Lee
authors.forename: Shane M
authors.initials: SM
authors.lastname: Hart
authors.forename: Matt G
authors.initials: MG
authors.lastname: Goering
authors.forename: Mark
authors.initials: M
authors.lastname: O'Neil-Johnson
authors.forename: Gary R
authors.initials: GR
authors.lastname: Eldridge
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D029792:Arctostaphylos; D006027:Glycosides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D006027:Glycosides; D010936:Plant Extracts