Glutathione-S-transferase and p53 polymorphisms in cervical carcinogenesis.
OBJECTIVE
To investigate the clinical significance of glutathione-S-transferase GSTM1, GSTT1 and p53 codon 72 polymorphisms in cervical carcinogenesis.


METHODS
GSTM1, GSTT1 and p53 codon 72 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 198 cervical smear samples using multiplex polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism (RFLP) techniques.


RESULTS
Forty-two patients with high-grade squamous intraepithelial lesion (HSIL) had higher frequency of high-risk HPV and null GSTT1 genotype than 102 with low-grade SIL (LSIL) and 54 controls. Thirty-one patients with HSIL had also statistically higher frequency of null GSTT1 genotype than 28 with LSIL among 69 patients with high-risk HPV. There was no statistical difference in p53 Arg, Arg/Pro and Pro genotypes between SILs and controls with or without high-risk HPV.


CONCLUSIONS
GSTT1 null genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population. The p53 codon 72 polymorphism is unlikely to be related to HPV status and the onset of cervical cancer.
Ueda
Masatsugu
M
Department of Obstetrics and Gynecology, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan. gyn017@poh.osaka-med.ac.jp
Hung
Yao-Ching
YC
Terai
Yoshito
Y
Saito
Junko
J
Nunobiki
Osamu
O
Noda
Sadamu
S
Ueki
Minoru
M
D002471:Cell Transformation, Neoplastic / Q000235:genetics; D003062:Codon / Q000235:genetics; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014412:Tumor Virus Infections / Q000150:complications / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000201:enzymology / Q000235:genetics* / Q000821:virology
D003062:Codon; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1