Functional variability of Rev response element in HIV-1 primary isolates.
We have previously studied sequence heterogeneity of HIV-1 Rev response element (RRE), and showed uneven variations in different stem-loops of both primary sequence and secondary structure. Here we studied the functional variation of RRE clones from a set of 10 primary isolates, and demonstrated a variation in the function of these RRE clones on the expression of Gag proteins from a truncated HIV-1 genome. The difference in Gag level was, in part, if not exclusively, resulted from the differential efficiency of RNA transport and enhancing of translation. These data suggested that variation of HIV-1 RRE may play a role in regulation of viral replication rate in HIV-1 primary isolates.
Phuphuakrat
Angsana
A
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Auewarakul
Prasert
P
D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000096:biosynthesis; D015752:Genes, env / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D034443:RNA Transport; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014779:Virus Replication
D015683:Gene Products, gag; D012367:RNA, Viral
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