Antigens recognized by autologous antibodies of patients with soft tissue sarcoma.
Serological analysis of recombinant cDNA libraries (SEREX) uses high titer IgGs to identify antigens expressed by autologous cancers. In order to identify tumor antigens in soft tissue sarcoma (STS), sera from four patients with malignant fibrous histiocytoma (MFH), gastrointestinal stromal tumor (GIST), pleomorphic liposarcoma, and dedifferentiated liposarcoma were screened against cDNA libraries derived from autologous tumor. We identified 18 antigens encoded by 15 different genes, including DLG7, which is located on chromosome 14q22, a locus previously found to be altered in STS, and the proto-oncogene JUN. Ten of fourteen antigens (71%) do not react with sera from healthy donors, suggesting that antibody recognition takes place during cancer progression. Using oligonucleotide microarray technology, most genes were variably expressed across a panel of 16 benign specimens and 41 STSs of different histologies. DLG7, however, showed restricted expression in testes and cancer, similarly to known germ cell cancer-testis antigens (or germ cell antigens, GCAs). Thus, the current study identified several antigens, including molecules implicated in tumorigenesis that were recognized by high titer IgGs in STS patients. Further studies of these selected novel STS antigens are warranted to identify and characterize potential antigen targets expressed by STS.
Segal
Neil H
NH
Swim Across America Laboratory, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. a-houghton@ski.mskcc.org
Blachere
Nathalie E
NE
Shiu
Ho Yin A
HY
Leejee
Suh
S
Antonescu
Cristina R
CR
Lewis
Jonathan J
JJ
Wolchok
Jedd D
JD
Houghton
Alan N
AN
D000912:Antibodies, Neoplasm / Q000097:blood / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D000090063:Proto-Oncogene Mas; D012509:Sarcoma / Q000235:genetics / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007074:Immunoglobulin G; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas