title: LC determination of citral in Cymbopogon citratus volatile oil.
abstract: It was the aim of this study to develop and validate a HPLC method for the quantitative determination of citral in Cymbopogon citratus volatile oil. The HPLC assay was performed using a Spherisorb CN column (250 mm x 4.6 mm, 5 microm), a n-hexane:ethanol (85:15) mobile phase and an UV detector (set at 233 nm). The following parameters were evaluated: linearity, precision, accuracy, specificity, quantification and detection limits. The method showed linearity in the range of 10.0-30.0 microg ml(-1). Precision and accuracy were determined at the concentration of 20 microg ml(-1). The concentration of citral in C. citratus volatile oil obtained with this assay was 75%. The HPLC method developed in this study showed an excellent performance (linearity, precision, accuracy and specificity) and can be applied to assay citral in volatile oil.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, CEP 90160-000 RS, Brazil. cristie@farmacia.ufrgs.br
authors.forename: Cristiane da S
authors.initials: Cda S
authors.lastname: Rauber
authors.forename: Sílvia S
authors.initials: SS
authors.lastname: Guterres
authors.forename: Elfrides E S
authors.initials: EE
authors.lastname: Schapoval
mesh_terms: D000080462:Acyclic Monoterpenes; D002853:Chromatography, Liquid / Q000379:methods; D031743:Cymbopogon; D039821:Monoterpenes / Q000032:analysis*; D009822:Oils, Volatile / Q000032:analysis*
chemical_list: D000080462:Acyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; C007076:citral