title: [Preparation of porcine acellular dermal matrix by low concentration of trypsin digestion and repeated freeze-thaw cycles].
abstract: OBJECTIVE
  To establish a new method for the preparation of porcine acellular dermal matrix.
  
  
  METHODS
  The antigenicity of the porcine dermis was weakened by removing epidermal and dermal cells from the porcine skin through the digestion with low-concentration trypsin and repeated freeze-thaw cycles. Split thickness porcine skin was treated with 0.05% trypsin to remove the cells from the epidermis and dermis. Repeated freeze-thaw cycles were employed to further weed out the residual cells within the dermis. The prepared acellular dermis was then examined grossly, as well as histologically, and also by immunohistochemical method.
  
  
  RESULTS
  No cell could be identified in the prepared porcine acellular dermal matrix. The integral basement membrane was preserved on the surface of dermal matrix with compact dermal matrix collagen structure.
  
  
  CONCLUSIONS
  Low concentration trypsinization and repeated freeze-thaw cycles seemed to be a simple and effective method for the preparation of xenogeneic acellular dermal matrix.
authors.affiliation: Department of Plastic Surgery, Gulou Hospital, The Medical School of Nanjing University, Nanjing 210008, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tan
authors.forename: Zhong-tao
authors.initials: ZT
authors.lastname: Zou
authors.forename: Guan-sen
authors.initials: GS
authors.lastname: Ning
authors.forename: Zi-hao
authors.initials: ZH
authors.lastname: Lin
authors.forename: Hong-reng
authors.initials: HR
authors.lastname: Zhou
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Liang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Wu
mesh_terms: D000818:Animals; D020405:Dermis / Q000166:cytology* / Q000637:transplantation; D005109:Extracellular Matrix / Q000637:transplantation / Q000648:ultrastructure*; D005615:Freezing; D016038:Skin Transplantation; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014357:Trypsin / Q000008:administration & dosage*
chemical_list: D014357:Trypsin