title: Potential of preliminary test methods to predict biodegradation performance of petroleum hydrocarbons in soil.
abstract: Preliminary tests at different scales such as degradation experiments (laboratory) in shaking flasks, soil columns and lysimeters as well as in situ respiration tests (field) were performed with soil from two hydrocarbon contaminated sites. Tests have been evaluated in terms of their potential to provide information on feasibility, degradation rates and residual concentration of bioremediation in the vadose zone. Sample size, costs and duration increased with experimental scale in the order shaking flasks - soil columns - lysimeter - in situ respiration tests, only time demand of respiration tests was relatively low. First-order rate constants observed in degradation experiments exhibited significant differences between both, different experimental sizes and different soils. Rates were in line with type and history of contamination at the sites, but somewhat overestimated field rates particularly in small scale experiments. All laboratory experiments allowed an estimation of residual concentrations after remediation. In situ respiration tests were found to be an appropriate pre-testing and monitoring tool for bioventing although residual concentrations cannot be predicted from in situ respiration tests. Moreover, this method does not account for potential limitations that might hamper biodegradation in the longer term but only reflects the actual degradation potential when the test is performed.
authors.affiliation: Department of Environmental Biotechnology, Institute for Agrobiotechnology Konrad Lorenz Strasse 20, 3430 Tulln, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Aichberger
authors.forename: Marion
authors.initials: M
authors.lastname: Hasinger
authors.forename: Rudolf
authors.initials: R
authors.lastname: Braun
authors.forename: Andreas P
authors.initials: AP
authors.lastname: Loibner
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D005240:Feasibility Studies; D006838:Hydrocarbons / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D010100:Oxygen / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism*; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D006838:Hydrocarbons; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D010100:Oxygen