title: IgE reactivity to vaccine components in dogs that developed immediate-type allergic reactions after vaccination.
abstract: Allergic reactions after vaccination are considered as an important practical problem in dogs; however, their immunological mechanism has not been well understood. The present study was designed to investigate the relationship between IgE reactivity to the vaccines and immediate-type allergic reactions after vaccination in dogs. Sera from 10 dogs that developed immediate-type allergic reactions such as circulatory collapse, cyanosis, dyspnea, facial edema, and vomiting within 1h after vaccination with non-rabies monovalent or combined vaccines and sera from 50 dogs that did not develop allergic reactions after vaccination were collected. Serum IgE reactivity to the injected vaccines was measured by fluorometric ELISA using a mouse monoclonal anti-dog IgE antibody. Then, IgE reactivity to fetal calf serum (FCS) and stabilizer proteins (gelatin, casein, and peptone) included in the vaccines was measured in sera that had high levels of IgE to the vaccines. Levels of serum specific IgE to the vaccines in dogs with immediate-type allergic reactions (59-4173 fluorescence units [FU], mean +/- S.D.: 992.5 +/- 1181.9 FU) were significantly higher than those in control dogs (38-192 FU, 92.4 +/- 43.3 FU) (P < 0.001). Of the eight dogs that developed immediate-type allergic reactions and had high levels of serum specific IgE to the vaccines, seven had specific IgE directed to FCS. The IgE reactivity to the vaccines in sera from these dogs was almost completely inhibited by FCS. The other one dog had serum IgE directed to gelatin and casein included in the vaccine as stabilizers. The results obtained in this study suggest that immediate-type allergic reactions after vaccination in dogs were induced by type I hypersensitivity mediated by IgE directed to vaccine components. In addition, FCS, gelatin, and casein included in vaccines could be the causative allergens that induced immediate-type allergic reactions after vaccination in dogs.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. aa27150@mail.ecc.u-tokyo.ac.jp
authors.forename: Keitaro
authors.initials: K
authors.lastname: Ohmori
authors.forename: Kenichi
authors.initials: K
authors.lastname: Masuda
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kaburagi
authors.forename: Keigo
authors.initials: K
authors.lastname: Kurata
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Deboer
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D002364:Caseins / Q000009:adverse effects / Q000276:immunology; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005470:Fluorometry; D005780:Gelatin / Q000009:adverse effects / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000662:veterinary*; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D012710:Serum Albumin, Bovine / Q000009:adverse effects / Q000032:analysis / Q000276:immunology; D014611:Vaccination / Q000009:adverse effects / Q000662:veterinary*; D017778:Vaccines, Combined / Q000009:adverse effects* / Q000737:chemistry / Q000276:immunology*
chemical_list: D002364:Caseins; D007074:Immunoglobulin G; D017778:Vaccines, Combined; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D005780:Gelatin
references.citation: Aust Vet J. 1991 Oct;68(10):342-4
references.pmid: 1755787
references.citation: J Allergy Clin Immunol. 1996 Dec;98(6 Pt 1):1058-61
references.pmid: 8977505
references.citation: J Am Vet Med Assoc. 1996 Aug 1;209(3):608-11
references.pmid: 8755979
references.citation: Vet Immunol Immunopathol. 2001 Nov;83(1-2):69-77
references.pmid: 11604162
references.citation: Int Arch Allergy Immunol. 2003 Apr;130(4):275-9
references.pmid: 12740528
references.citation: J Allergy Clin Immunol. 1995 Oct;96(4):563-5
references.pmid: 7560672
references.citation: J Vet Med Sci. 2002 Sep;64(9):851-3
references.pmid: 12399614
references.citation: Adv Vet Med. 1999;41:681-700
references.pmid: 9890054
references.citation: J Allergy Clin Immunol. 1997 May;99(5):720-1
references.pmid: 9155843
references.citation: Am J Vet Res. 2002 Apr;63(4):611-6
references.pmid: 11939328
references.citation: Vet Immunol Immunopathol. 1993 Aug;37(3-4):183-99
references.pmid: 8236798
references.citation: J Allergy Clin Immunol. 1993 Apr;91(4):867-72
references.pmid: 8473675