In vitro toxicity of selected pesticides on RTG-2 and RTL-W1 fish cell lines.
The rainbow trout fish cell lines RTG-2 and RTL-W1 were used to determine the cytotoxic effects of the pesticides bifenthrin, cypermethrin, cyhalothrin, lambda-cyhalothrin, quinalphos and chlorpyrifos. Cytotoxicity was measured by EROD and beta-Gal enzymatic activities, the neutral red (NR) uptake assay, and the FRAME KB protein (KBP) assay. The beta-Gal activity was unaffected by the pesticide exposure. The EROD activity was induced by cyhalothrin and lambda-cyhalothrin (RTG-2 and RTL-W1) and by bifenthrin (RTL-W1). Dose dependent inhibition responses were observed for EROD activity in cells exposed to quinalphos (RTL-W1) and chlorpyrifos (RTG-2 and RTL-W1). RTL-W1 offered a better response for EROD induction. The EC50 values on EROD endpoint were more sensitive than NR and KBP. The acute fish toxicity of chlorpyrifos and quinalphos depends highly on the species; the species sensitivity distributions cover several orders of magnitude and the values obtained for EROS were within the lowest part of the reported ranges.
Babín
M M
MM
Laboratory for Ecotoxicology, Department of the Environment, INIA, Crta. de La Coruña Km 7, 28040 Madrid, Spain. babin@inia.es
Tarazona
J V
JV
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D009499:Neutral Red; D010575:Pesticides / Q000633:toxicity*; D013045:Species Specificity; D023382:Toxicity Tests, Acute / Q000379:methods; D014337:Trout / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D001616:beta-Galactosidase / Q000378:metabolism
D010575:Pesticides; D014874:Water Pollutants, Chemical; D009499:Neutral Red; D019363:Cytochrome P-450 CYP1A1; D001616:beta-Galactosidase