title: [Separation of soybean agglutinin by hydrophobic-interaction chromatography].
abstract: The soybean agglutinin (SBA) was separated with hydrophobic-interaction chromatography (HIC) in this work. HIC of the crude lectin extract of SBA was performed on Phenyl-Sepharose CL-4B column, 10 cm x 2.5 cm i.d., at a flow-rate of 1.0 mL/min. A 20 mL sample of the crude extract was applied to the column previously equilibrated with the buffer, 0.01 mol/L PBS containing ammonium sulfate (60% of saturation concentration). The column was eluted with the starting buffer, then with the buffer containing 30% saturation of ammonium sulfate, and finally with 0.01 mol/L PBS. Fractions were detected by UV absorbance at 280 nm and assayed for hemagglutinating activity. The results show that the purification-fold was as high as 35 and the recovery of activity was 100%.
authors.affiliation: School of Chemical Engineering, Dalian University of Technology, Dalian, 116012.
authors.forename: Y
authors.initials: Y
authors.lastname: Cheng
authors.forename: H
authors.initials: H
authors.lastname: Hu
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Zhao
authors.forename: Z
authors.initials: Z
authors.lastname: Su
mesh_terms: D002845:Chromatography / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification*; D030262:Soybean Proteins / Q000737:chemistry / Q000302:isolation & purification*; D010835:Phytohemagglutinins
chemical_list: D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins