Molecular characterization of a strain of cucumber mosaic virus based on coat protein and movement protein genes.
Cucumber mosaic virus (CMV) A strain (CMV-A) isolated from Amaranthus tricolor was partially characterized at molecular level. Complete coat protein (CP) and movement protein (MP) ORFs were cloned and sequenced. The 657 bp region of CP gene and the 840 bp region of MP gene encode 218 and 276 amino acids, respectively. CP, at nucleotide level, showed 90-98% sequence identity with the CMV subgroup I and less than 80% with the CMV subgroup II, it showed at amino acid level 92-96% identity with the subgroup I and 74-87% with the subgroup II. The nucleotide and amino acid sequence identities of MP ranged in 91-94% and 92-96%, respectively with the subgroup I but in 81-83% with the subgroup II. Phylogenetic trees generated from nucleotide and amino acid sequences of both CP and MP genes identified the virus strain as a member of the subgroup IB. CMV-A CP also displayed a remarkably higher homology with Indian strains of CMV than with other CMV strains and formed a separate cluster within the subgroup IB.
Srivastava
A
A
Molecular Virology Division, National Botanical Research Institute, Lucknow-220 001, India.
Chandra
G
G
Raj
S K
SK
D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000235:genetics*; D017799:Cucumovirus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins / Q000235:genetics*
D036022:Capsid Proteins; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins