[Determination of ligustilide in volatile oil from rhizome of ligusticum chuanxiong by RP-HPLC].
OBJECTIVE
To determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC.


METHODS
ODS2 column (4.6 mm x 200 mm, 5 microm) was used and nitrendipine was used as internal standard. The mobil phase consisted methanol, acetontrile and water (33:21:46). The ligustilide was at 275 nm.


RESULTS
The linear range was 2.92-29.2 mg x L(-1) for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%.


CONCLUSIONS
This method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility.
Lin
Yan-zhi
YZ
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Tang
Xing
X
Bi
Kai-shun
KS
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030004:Ligusticum / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
D009822:Oils, Volatile; C027820:ligustilide; D015107:4-Butyrolactone