Layer-by-layer assembly of human serum albumin and phospholipid nanotubes based on a template.
The preparation of nanotubes from human serum albumin (HSA) and mixtures of L-alpha-dimyristoylphosphatidic acid (DMPA)/HSA is described. The nanotubes were prepared via alternate adsorption of HSA of different/opposite charges (by variation of the pH) or by sequential adsorption of DMPA and HSA, respectively, onto the inner surfaces of porous anodic alumina templates. This simple layer-by-layer assembly results in a monodisperse size distribution and a uniform orientation. The nanotubes allow the specific incorporation of lipophilic components such as channels or receptors and may thus serve as probes or sensors for biological systems.
Lu
Gang
G
International Joint Lab, Key Lab of Colloid and Interface Science, The Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Zhong Guan Cun, Beijing 100080, China.
Ai
Sufen
S
Li
Junbai
J
D002942:Circular Dichroism; D020404:Glycerophospholipids / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D012709:Serum Albumin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D020404:Glycerophospholipids; D012709:Serum Albumin; C025602:dimyristoylphosphatidic acid