title: Actin depolymerization transduces the strength of B-cell receptor stimulation.
abstract: Polymerization of the actin cytoskeleton has been found to be essential for B-cell activation. We show here, however, that stimulation of BCR induces a rapid global actin depolymerization in a BCR signal strength-dependent manner, followed by polarized actin repolymerization. Depolymerization of actin enhances and blocking actin depolymerization inhibits BCR signaling, leading to altered BCR and lipid raft clustering, ERK activation, and transcription factor activation. Furthermore actin depolymerization by itself induces altered lipid raft clustering and ERK activation, suggesting that F-actin may play a role in separating lipid rafts and in setting the threshold for cellular activation.
authors.affiliation: Immunology Research Laboratories, Department of Veterinary Science, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Shengli
authors.initials: S
authors.lastname: Hao
authors.forename: Avery
authors.initials: A
authors.lastname: August
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D020013:Calcium Signaling; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D008213:Lymphocyte Activation; D020935:MAP Kinase Signaling System; D021962:Membrane Microdomains / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000199:Actins; D001704:Biopolymers; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D011947:Receptors, Antigen, B-Cell; D014157:Transcription Factors
references.citation: Cell. 1999 Jan 8;96(1):9-12
references.pmid: 9989492
references.citation: Science. 1983 Feb 4;219(4584):493-5
references.pmid: 6681676
references.citation: Cell. 1999 Jul 23;98(2):159-69
references.pmid: 10428028
references.citation: J Cell Biol. 1999 Oct 18;147(2):447-61
references.pmid: 10525547
references.citation: J Exp Med. 1999 Nov 1;190(9):1329-42
references.pmid: 10544204
references.citation: J Exp Med. 1999 Dec 6;190(11):1549-60
references.pmid: 10587346
references.citation: J Exp Med. 2000 May 1;191(9):1591-604
references.pmid: 10790433
references.citation: Nature. 2000 Apr 27;404(6781):999-1003
references.pmid: 10801129
references.citation: Eur J Biochem. 2000 Jul;267(14):4339-45
references.pmid: 10880956
references.citation: J Immunol. 2000 Aug 1;165(3):1220-7
references.pmid: 10903719
references.citation: J Biol Chem. 2000 Sep 22;275(38):29183-6
references.pmid: 10918051
references.citation: J Immunol. 2000 Dec 1;165(11):6020-3
references.pmid: 11086032
references.citation: Cell Motil Cytoskeleton. 2001 Jan;48(1):37-51
references.pmid: 11124709
references.citation: Eur J Immunol. 2001 Feb;31(2):345-9
references.pmid: 11180097
references.citation: J Biol Chem. 2001 Jan 19;276(3):1715-9
references.pmid: 11042193
references.citation: J Immunol. 2001 Mar 15;166(6):3855-64
references.pmid: 11238629
references.citation: FEBS Lett. 1987 Mar 23;213(2):316-8
references.pmid: 3556584
references.citation: J Cell Biol. 1987 Oct;105(4):1473-8
references.pmid: 3312229
references.citation: Cell Motil Cytoskeleton. 1989;13(3):127-44
references.pmid: 2776221
references.citation: J Biol Chem. 1994 May 27;269(21):14869-71
references.pmid: 8195116
references.citation: Science. 1994 Jun 24;264(5167):1948-51
references.pmid: 7516582
references.citation: Cell. 1994 Aug 26;78(4):635-44
references.pmid: 8069912
references.citation: J Exp Med. 1995 Feb 1;181(2):577-84
references.pmid: 7836913
references.citation: Cell. 1996 Mar 8;84(5):723-34
references.pmid: 8625410
references.citation: Immunity. 1996 Jul;5(1):41-52
references.pmid: 8758893
references.citation: Mol Biol Cell. 1997 Feb;8(2):341-51
references.pmid: 9190212
references.citation: Immunity. 1997 Apr;6(4):419-28
references.pmid: 9133421
references.citation: J Cell Biol. 1998 Feb 9;140(3):699-707
references.pmid: 9456328
references.citation: Eur J Immunol. 1998 Feb;28(2):716-29
references.pmid: 9521082
references.citation: Biophys J. 1998 Dec;75(6):3101-9
references.pmid: 9826630
references.citation: J Biol Chem. 1998 Dec 11;273(50):33588-94
references.pmid: 9837942
references.citation: Science. 1999 Jan 29;283(5402):680-2
references.pmid: 9924026
references.citation: J Immunol. 1999 Feb 15;162(4):2243-50
references.pmid: 9973500
references.citation: Nat Immunol. 2000 Jul;1(1):23-9
references.pmid: 10881170
references.citation: Nat Immunol. 2000 Oct;1(4):348-52
references.pmid: 11017108
references.citation: Nat Immunol. 2001 Jun;2(6):548-55
references.pmid: 11376343
references.citation: Traffic. 2001 Jun;2(6):414-27
references.pmid: 11389769
references.citation: J Biol Chem. 2001 Aug 3;276(31):29313-8
references.pmid: 11390365
references.citation: J Immunol. 2002 Jan 15;168(2):541-4
references.pmid: 11777944
references.citation: Eur J Immunol. 2002 Feb;32(2):435-46
references.pmid: 11813162
references.citation: Br J Pharmacol. 2002 Jul;136(6):837-46
references.pmid: 12110608
references.citation: EMBO J. 2002 Nov 1;21(21):5611-22
references.pmid: 12411479
references.citation: Mol Biol Cell. 2003 Feb;14(2):432-44
references.pmid: 12589045
references.citation: Curr Opin Immunol. 2003 Jun;15(3):255-60
references.pmid: 12787749
references.citation: EMBO J. 2003 Aug 15;22(16):4166-77
references.pmid: 12912915
references.citation: Science. 2003 Oct 17;302(5644):445-9
references.pmid: 14564009
references.citation: Science. 2003 Oct 17;302(5644):459-62
references.pmid: 14564011
references.citation: Exp Cell Res. 2003 Nov 15;291(1):11-24
references.pmid: 14597404
references.citation: Mol Cell Biol. 2004 Feb;24(4):1628-39
references.pmid: 14749378
references.citation: Science. 1999 Jul 9;285(5425):221-7
references.pmid: 10398592