title: Xylanases and carboxymethylcellulases of the rumen protozoa Polyplastron multivesiculatum, Eudiplodinium maggii and Entodinium sp.
abstract: Endoglucanase and xylanase activities of three rumen protozoa, Polyplastron multivesiculatum, Eudiplodinium maggii, and Entodinium sp. were compared qualitatively by zymograms and quantitatively by measuring specific activities against different polysaccharides. A set of carboxymethylcellulases and xylanases was produced by the large ciliates whereas no band of activity was observed for Entodinium sp. in zymograms. Specific activity of endoglucanases from P. multivesiculatum (1.3 micromol mg prot(-1) min(-1)) was twice that of E. maggii, whereas xylanase specific activity (4.5 micromol mg prot(-1) min(-1)) was only half. Very weak activities were observed for Entodinium sp. A new xylanase gene, xyn11D, from P. multivesiculatum was reported and its gene product compared to 33 other family 11 xylanases. Phylogenetic analysis showed that xylanase sequences from rumen protozoa are closely related to those of bacteria.
authors.affiliation: Unité de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France. cmaillet@clermont.inra.fr
authors.forename: Christel
authors.initials: C
authors.lastname: Béra-Maillet
authors.forename: Estelle
authors.initials: E
authors.lastname: Devillard
authors.forename: Magalie
authors.initials: M
authors.lastname: Cezette
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jouany
authors.forename: Evelyne
authors.initials: E
authors.lastname: Forano
mesh_terms: D000818:Animals; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D016798:Ciliophora / Q000201:enzymology* / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism*; D017125:Genes, Protozoan; D010802:Phylogeny; D012417:Rumen / Q000469:parasitology*; D012756:Sheep; D013045:Species Specificity
chemical_list: D002480:Cellulase; C019826:carboxymethylcellulase; D043364:Endo-1,4-beta Xylanases