[Freeze-drying of silymarin-loaded solid lipid nanoparticles (SM-SLN)].
OBJECTIVE
To investigate lyophilization of SM-SLN.


METHODS
The parameters of lyophilization process was optimized. In addition, the protective effect of various types and concentrations of cryoprotectants were tested by shape, colour and disparity.


RESULTS
The mixture of 2% lactose and 2% glucose could better prevent nanoparticles from aggregating, the optimal lyophilization process was followed: precooled at -45 degrees C for 10 hr; primary drying at -25 degrees C for 5 hr; secondary drying at 10 degrees C for 3 hr; finally drying at 30 degrees C for 6 hr.


CONCLUSIONS
Changes in particle size distribution during lyophilization could be minimized by optimizing the parameters of the lyophilization process and adding supporting agent.
He
Jun
J
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Feng
Jian-fang
JF
Zhang
Le-le
LL
Lu
Wei-gen
WG
Hou
Shi-xiang
SX
D004337:Drug Carriers / Q000737:chemistry*; D005612:Freeze Drying / Q000379:methods; D005947:Glucose / Q000737:chemistry; D007785:Lactose / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D020944:Silybum marianum / Q000737:chemistry; D036103:Nanotechnology; D010316:Particle Size; D010946:Plants, Medicinal / Q000737:chemistry; D012838:Silymarin / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D013678:Technology, Pharmaceutical / Q000379:methods*
D004337:Drug Carriers; D008055:Lipids; D012838:Silymarin; D005947:Glucose; D007785:Lactose