Endothelial cell--interactions with polyelectrolyte multilayer films.
The seeding of endothelial cells (ECs) on biomaterial surfaces became a major challenge, allowing to improve the non-thrombogenic properties of these surfaces. Recently, the use of polyelectrolyte films has been suggested as a new versatile technique of surface modification aimed at tissue engineering. In this study, we evaluate the adhesion properties of ECs on two types of polyelectrolyte films ending either by poly(D-lysine) (PDL), or poly(allylamine hydrochloride) (PAH), and compared them to data obtained on PDL or PAH monolayers, glass and fibronectin (Fn)-coated glass. ECs seeded on polyelectrolyte films showed a good morphology, allowing ECs to resist physiological shear stress better compared to ECs seeded on glass or Fn. The expression of beta1 integrins was slightly lower on polyelectrolyte films than on control surfaces. However, the phosphorylation of focal adhesion kinase, involved in the transduction of adhesion signal, was not modified on PAH ending films compared to control surfaces; whereas it became lower on PDL ending films. Finally, PAH ending films improve strongly ECs adhesion without disturbing the adhesion mechanism, necessary for the development of a new endothelium. These types of films or similar build-ups could thus be used in the future as a way to modify surfaces for vascular tissue engineering.
Boura
Cédric
C
Laboratoire de Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR CNRS 7563, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy, France.
Muller
Sylvaine
S
Vautier
Dominique
D
Dumas
Dominique
D
Schaaf
Pierre
P
Claude Voegel
Jean
J
François Stoltz
Jean
J
Menu
Patrick
P
D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D004573:Electrolytes; D004730:Endothelium, Vascular / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006801:Humans; D016023:Integrins / Q000378:metabolism; D018625:Microscopy, Atomic Force; D011505:Protein-Tyrosine Kinases / Q000378:metabolism
D000199:Actins; D004573:Electrolytes; D016023:Integrins; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human