title: TOM software toolbox: acquisition and analysis for electron tomography.
abstract: Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. "TOM software toolbox" integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.
authors.affiliation: Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany. Nickell@biochem.mpg.de
authors.forename: Stephan
authors.initials: S
authors.lastname: Nickell
authors.forename: Friedrich
authors.initials: F
authors.lastname: Förster
authors.forename: Alexandros
authors.initials: A
authors.lastname: Linaroudis
authors.forename: William Del
authors.initials: WD
authors.lastname: Net
authors.forename: Florian
authors.initials: F
authors.lastname: Beck
authors.forename: Reiner
authors.initials: R
authors.lastname: Hegerl
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumeister
authors.forename: Jürgen M
authors.initials: JM
authors.lastname: Plitzko
mesh_terms: D000465:Algorithms; D006801:Humans; D007091:Image Processing, Computer-Assisted; D012984:Software; D014054:Tomography / Q000295:instrumentation / Q000379:methods*; D014584:User-Computer Interface