title: Analytical relationships among Biosite, Bayer, and Roche methods for BNP and NT-proBNP.
abstract: This study determined whether, for patient monitoring, it is feasible to convert B-type natriuretic peptide (BNP) results obtained using Triage (BNP, Biosite, San Diego, CA), Centaur (BNP, Bayer Diagnostics, Tarrytown, NY), and Elecsys 2010 (N-terminal proBNP; Roche, Indianapolis, IN) assays. Concordance between assays and effects of renal impairment also were assessed. Samples were primarily from emergency center patients. Biosite testing was performed immediately; Bayer and Roche testing was performed later on plasma stored frozen (-30 degrees C). Logistic regression relationships were as follows: Bayer = 0.57 Biosite + 23.1, n = 121, R2 = 0.85; Roche = 6.09 Biosite -220.4 + 1,131.6 (if female), n = 131, R2 = 0.57; and Roche = 15.34 Bayer + 2,400.8, n = 150, R2 = 0.23. An increased serum creatinine level (>/=2 mg/dL [>/=177 micromol/L]) influenced the Roche results. We conclude the following from this preliminary study: (1) Results from one method cannot be converted reliably to another using regression relationships. (2) When using manufacturers'cutoff values, concordance between assays was acceptable. (3) Renal impairment affected Roche results.
authors.affiliation: Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, MI 48073, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sykes
authors.forename: Raymond E
authors.initials: RE
authors.lastname: Karcher
authors.forename: Janice
authors.initials: J
authors.lastname: Eisenstadt
authors.forename: David A
authors.initials: DA
authors.lastname: Tushman
authors.forename: Mamtha
authors.initials: M
authors.lastname: Balasubramaniam
authors.forename: John
authors.initials: J
authors.lastname: Gusway
authors.forename: Valerie J
authors.initials: VJ
authors.lastname: Perason
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001322:Autoanalysis; D015415:Biomarkers / Q000032:analysis; D002624:Chemistry, Clinical / Q000379:methods; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000175:diagnosis; D006801:Humans; D008297:Male; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain; D045265:Natriuretic Peptides / Q000097:blood*; D009419:Nerve Tissue Proteins / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D045265:Natriuretic Peptides; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain