Transcriptional regulation of FKLF-2 (KLF13) gene in erythroid cells.
FKLF-2 (KLF13) was cloned from fetal globin-expressing tissues and has been shown to be abundantly expressed in erythroid cells. In this study we examined the transcriptional regulation of the KLF13 gene. A 5.5 kb 5' flanking region cloned from mouse erythroleukemia (MEL) cell genomic DNA showed that major cis regulatory activities exist in the 550 bp sequence to the unique transcription start site, and that the promoter is more active in K562 cells than in COS-7 cells. The promoter was trans-activated by co-expressed GATA-1 through the sequence containing two CCAAT motifs, suggesting that GATA-1 is involved in the abundant expression of KLF13 mRNA in the erythroid tissue. Dual action, i.e. activating effect in COS-7 and repressive effect in K562 cell, was observed on its own promoter, suggesting a feedback mechanism for the transcriptional control of the KLF13 gene in the erythroid environment. These findings provide an insight on the mechanism of inducible mRNA expression of the KLF13 gene in erythroid cells.
Mitsuma
Ayako
A
Department of Hematology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Asano
Haruhiko
H
Kinoshita
Tomohiro
T
Murate
Takashi
T
Saito
Hidehiko
H
Stamatoyannopoulos
George
G
Naoe
Tomoki
T
D000818:Animals; D019556:COS Cells; D018797:Cell Cycle Proteins / Q000235:genetics*; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020014:K562 Cells; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
D018797:Cell Cycle Proteins; C486423:KLF13 protein, human; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D012097:Repressor Proteins
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