title: An atomic resolution view of ICAM recognition in a complex between the binding domains of ICAM-3 and integrin alphaLbeta2.
abstract: Within the Ig superfamily (IgSF), intercellular adhesion molecules (ICAMs) form a subfamily that binds the leukocyte integrin alphaLbeta2. We report a 1.65-A-resolution crystal structure of the ICAM-3 N-terminal domain (D1) in complex with the inserted domain, the ligand-binding domain of alphaLbeta2. This high-resolution structure and comparisons among ICAM subfamily members establish that the binding of ICAM-3 D1 onto the inserted domain represents a common docking mode for ICAM subfamily members. The markedly different off-rates of ICAM-1, -2, and -3 appear to be determined by the hydrophobicity of residues that surround a metal coordination bond in the alphaLbeta2-binding interfaces. Variation in composition of glycans on the periphery of the interfaces influences on-rate.
authors.affiliation: CBR Institute for Biomedical Research, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Song
authors.forename: Yuting
authors.initials: Y
authors.lastname: Yang
authors.forename: Jin-Huan
authors.initials: JH
authors.lastname: Liu
authors.forename: Jose M
authors.initials: JM
authors.lastname: Casasnovas
authors.forename: Motomu
authors.initials: M
authors.lastname: Shimaoka
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Springer
authors.forename: Jia-Huai
authors.initials: JH
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1 / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D020349:Surface Plasmon Resonance
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; C483616:ICAM3 protein, human; D016169:Lymphocyte Function-Associated Antigen-1
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