title: Narrow-bore high performance liquid chromatographic method for the determination of cetirizine in human plasma using column switching.
abstract: An improved column switching high performance liquid chromatographic (HPLC) method was developed for determination of cetirizine in human plasma. Plasma samples were prepared by liquid-liquid extraction using methylene chloride. The samples extracted were initially injected into a clean-up Capcell Pak MF C8 column and the peaks of cetirizine and internal standard were separated to an analytical C18 micro-column via column switching device. This analysis showed highly sensitive and selective results. Also, it was successfully applied to evaluate the pharmacokinetics of cetirizine in human volunteers after single oral administration.
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong Kwanak-gu, Seoul 151-742, South Korea. ckkim@plaza.snu.ac.kr
authors.forename: Chong-Kook
authors.initials: CK
authors.lastname: Kim
authors.forename: Kyu Jeong
authors.initials: KJ
authors.lastname: Yeon
authors.forename: Eunmi
authors.initials: E
authors.lastname: Ban
authors.forename: Myung-Ja
authors.initials: MJ
authors.lastname: Hyun
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Kim
authors.forename: Mee-Kyung
authors.initials: MK
authors.lastname: Kim
authors.forename: Su-Eon
authors.initials: SE
authors.lastname: Jin
authors.forename: Jeong-Sook
authors.initials: JS
authors.lastname: Park
mesh_terms: D017332:Cetirizine / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans
chemical_list: D017332:Cetirizine