title: Rearrangements of large-insert T-DNAs in transgenic rice.
abstract: Introduction of large-DNA fragments into cereals by Agrobacterium-mediated transformation is a useful technique for map-based cloning and molecular breeding. However, little is known about the organization and stability of large fragments of foreign DNA introduced into plant genomes. In this study, we produced transgenic rice plants by Agrobacterium-mediated transformation with a large-insert T-DNA containing a 92-kb region of the wheat genome. The structures of the T-DNA in four independent transgenic lines were visualized by fluorescence in situ hybridization on extended DNA fibers (fiber FISH). By using this cytogenetic technique, we showed that rearrangements of the large-insert T-DNA, involving duplication, deletion and insertion, had occurred in all four lines. Deletion of long stretches of the large-insert DNA was also observed in Agrobacterium.
authors.affiliation: Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka, 582-8582, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakano
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.forename: Maki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Kym
authors.initials: K
authors.lastname: Turnbull
authors.forename: Sadequr
authors.initials: S
authors.lastname: Rahman
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Mukai
mesh_terms: D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D015321:Gene Rearrangement / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012231:Rhizobium; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; C036483:T-DNA
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