title: Design and performance of a sheathless capillary electrophoresis/mass spectrometry interface by combining fused-silica capillaries with gold-coated nanoelectrospray tips.
abstract: A simple sheathless capillary electrophoresis (CE)/mass spectrometry (MS) interface was constructed by combining widely used nanospray needles with fused-silica capillaries and it was successfully applied for the separation of peptides. The end of the CE capillary was pulled to a taper, etched and then fitted into the metal-coated nanospray borosilicate capillary. The nanospray needle can be used for several CE runs, but it can be easily and rapidly changed in the case of accidental breakage or evaporation of the coating. A fast capillary electrochromatographic method was also developed for MS analysis of peptides containing numerous basic amino acids.
authors.affiliation: Department of Medical Chemistry, University of Szeged, 6720 Szeged, Dóm tér 8, Hungary. kele@chem.u-szeged.hu
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Kele
authors.forename: Györgyi
authors.initials: G
authors.lastname: Ferenc
authors.forename: Eva
authors.initials: E
authors.lastname: Klement
authors.forename: Gábor K
authors.initials: GK
authors.lastname: Tóth
authors.forename: Tamás
authors.initials: T
authors.lastname: Janáky
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D036103:Nanotechnology; D010455:Peptides / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D010455:Peptides; D006046:Gold; D012822:Silicon Dioxide