title: Structural analysis of the oligosaccharide of Histophilus somni (Haemophilus somnus) strain 2336 and identification of several lipooligosaccharide biosynthesis gene homologues.
abstract: The structure of the core oligosaccharide from a pneumonic Histophilus somni (Haemophilus somnus) strain 2336 was elucidated. The lipooligosaccharide (LOS) was subjected to a variety of degradative procedures. The structures of the purified products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. The following structure for the core oligosaccharide was determined on the basis of the combined data from these experiments: [formula-see text]. The structural elucidation was intriguing as it suggested several differences in the LOS structures between strain 2336 and the related strain 738. Strain 738 originated following passaging of strain 2336 through a calf. The differences between the two structures are a different linkage between Gal II and GlcNAc (1-->4 here; 1-->3 in 738), the absence of phosphocholine (PCho) from 2336 and the presence of two phosphoethanolamine (PEtn) residues and Gal III (at the 2-position) of Hep II in 2336. Although pulse-field gel electrophoresis data following digest with only one restriction enzyme showed identical profiles suggesting that strains 738 and 2336 are the same strain, the structural data does suggest that, if strain 738 is indeed a phase variant of strain 2336, considerable variation occurred on calf passaging and could therefore be an intriguing example of how broadly this bacterium can adapt itself in the host.
authors.affiliation: Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ontario, Canada K1A 0R6.
authors.forename: Frank
authors.initials: F
authors.lastname: St Michael
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Michael D
authors.initials: MD
authors.lastname: Howard
authors.forename: A Jane
authors.initials: AJ
authors.lastname: Duncan
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Inzana
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Cox
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D014644:Genetic Variation; D044138:Haemophilus somnus / Q000737:chemistry* / Q000235:genetics*; D013058:Mass Spectrometry; D008745:Methylation; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D009844:Oligosaccharides