title: Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling.
abstract: The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (T(H)1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse T(H)1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.
authors.affiliation: Max Planck Institute of Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Gutjahr
authors.forename: Derek
authors.initials: D
authors.lastname: Murphy
authors.forename: Angelika
authors.initials: A
authors.lastname: Lueking
authors.forename: Andrea
authors.initials: A
authors.lastname: Koenig
authors.forename: Michal
authors.initials: M
authors.lastname: Janitz
authors.forename: John
authors.initials: J
authors.lastname: O'Brien
authors.forename: Bernhard
authors.initials: B
authors.lastname: Korn
authors.forename: Sabine
authors.initials: S
authors.lastname: Horn
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Dolores J
authors.initials: DJ
authors.lastname: Cahill
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling; D051379:Mice; D008822:Mice, Transgenic; D018417:Th1 Cells / Q000276:immunology*
chemical_list: D000906:Antibodies; D017931:DNA Primers; D018076:DNA, Complementary