Fibrillogenesis of apomyoglobin facilitated by aggregation sequence of yeast Sup35 in various regions.
To examine the effect of aggregation sequence QGGYQQQYNP from yeast Sup35 on fibril formation of sperm whale apomyoglobin (apoMb), we constructed several mutants via substitution. Urea-induced unfolding of apoMb confirms that the substitution of the aggregation sequence does not significantly affect the stability of the mutants compared to wild type (WT) at pH 4.2. Under this condition, however, despite the difference in rate most apoMb mutants form fibrils more readily than WT with distinct morphology. These results suggest that the aggregation sequence facilitates fibril assembly of apoMb at acidic pH in vitro and this facilitation depends on the regions replaced.
He
Yingbo
Y
Laboratory of Protein Chemistry, MOE Laboratory of Protein Science, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China.
Tang
Huadong
H
Yi
Zhiwei
Z
Zhou
Hao
H
Luo
Yongzhang
Y
D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D052160:Benzothiazoles; D006863:Hydrogen-Ion Concentration; D008297:Male; D008854:Microscopy, Electron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009211:Myoglobin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D010454:Peptide Termination Factors; D011328:Prions / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011489:Protein Denaturation / Q000187:drug effects; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013844:Thiazoles; D014508:Urea / Q000494:pharmacology; D014907:Whales
D001059:Apoproteins; D052160:Benzothiazoles; D009211:Myoglobin; D010454:Peptide Termination Factors; D011328:Prions; D011993:Recombinant Fusion Proteins; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013844:Thiazoles; C010857:apomyoglobin; C009462:thioflavin T; D014508:Urea