title: Proton binding within a membrane protein by a protonated water cluster.
abstract: Proton transfer is crucial for many enzyme reactions. Here, we show that in addition to protonatable amino acid side chains, water networks could constitute proton-binding sites in proteins. A broad IR continuum absorbance change during the proton pumping photocycle of bacteriorhodopsin (bR) indicates most likely deprotonation of a protonated water cluster at the proton release site close to the surface. We investigate the influence of several mutations on the proton release network and the continuum change, to gain information about the location and extent of the protonated water network and to reveal the participating residues necessary for its stabilization. We identify a protonated water cluster consisting in total of one proton and about five water molecules surrounded by six side chains and three backbone groups (Tyr-57, Arg-82, Tyr-83, Glu-204, Glu-194, Ser-193, Pro-77, Tyr-79, and Thr-205). The observed perturbation of proton release by many single-residue mutations is now explained by the influence of numerous side chains on the protonated H bonded network. In situ hydrogen/deuterium exchange Fourier transform IR measurements of the bR ground state, show that the proton of the release group becomes localized on Glu-204 and Asp-204 in the ground state of the mutants E194D and E204D, respectively, even though it is delocalized in the ground state of wild-type bR. Thus, the release mechanism switches between the wild-type and mutated proteins from a delocalized to a localized proton-binding site.
authors.affiliation: Lehrstuhl für Biophysik, Ruhr-Universität Bochum, D-44780 Bochum, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Garczarek
authors.forename: Leonid S
authors.initials: LS
authors.lastname: Brown
authors.forename: Janos K
authors.initials: JK
authors.lastname: Lanyi
authors.forename: Klaus
authors.initials: K
authors.lastname: Gerwert
mesh_terms: D001665:Binding Sites; D006860:Hydrogen Bonding; D008565:Membrane Proteins / Q000737:chemistry*; D011522:Protons; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins; D011522:Protons; D014867:Water
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