[Effects of allitridin on the expression of human cytomegalovirus immediate early antigens-IE72 and IE86 in human embryonic lung cells].
OBJECTIVE
To investigate the effect of allitridin injection on the expression of human cytomegalovirus (HCMV) immediate-early antigens (IEAs including IE72 and IE86) in human embryonic lung cells.


METHODS
HCMV AD 169 Virus strain infected cell model (MOI = 2.5 and 0.25, respectively) were established, and then treated with ICm5 and MTC doses of allitridin. Western blot was used to analyze the of IE72 and IE86 expression after the treatment, ganciclovir(GCV, IC50 and 2.3 x IC50) treatment as control.


RESULTS
No matter what kind of MOI was used, both IE86 and IE72 antigens' expression was effectively suppressed by allitridin treatment, and the inhibitory rate of IE86 was almost twice of IE72's. Compared with GCV, allitridin had stronger inhibitory effect on IE86 expressing, although its efficacy on IE72 was weaker than GCV.


CONCLUSIONS
Allitridin could suppress the expression of IE72 and IE86, especially for IE86 expressing, maybe it is ore of key role in the mechanism of allitridin against HCMV.
Zhen
Hong
H
Pediatric Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Fang
Feng
F
Liu
Zhi-Feng
ZF
Nie
Xing-Cao
XC
Cui
Wen
W
Li
Ge
G
D000498:Allyl Compounds / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005737:Garlic / Q000737:chemistry; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D007267:Injections; D008168:Lung / Q000166:cytology; D013440:Sulfides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D015534:Trans-Activators / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D000498:Allyl Compounds; D000998:Antiviral Agents; C088645:IE1 protein, cytomegalovirus; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D013440:Sulfides; D015534:Trans-Activators; D014764:Viral Proteins; C042577:diallyl trisulfide