Analysis of polyamine metabolism in soybean seedlings using 15N-labelled putrescine.
The translocation and metabolism of polyamines during soybean germination were studied using 15N-labelled putrescine as a precursor. Both 15N-labelled and unlabelled polyamines were simultaneously detected using a novel application of ionspray ionization-mass spectrometry. 15N-putrescine was rapidly transported to the shoots and roots, where it was converted to spermidine and spermine. The main 15N-polyamine that accumulated in the root was 15N-spermine. It was found that there were differences in the way endogenous putrescine and exogenous 15N-putrescine were metabolized in soybean seedlings.
Ohe
Masato
M
Department of Biochemistry, Dokkyo University School of Medicine, Mibu, Tochigi 321 0293, Japan. masatooe@dokkyomed.ac.jp
Kobayashi
Masaki
M
Niitsu
Masaru
M
Bagni
Nello
N
Matsuzaki
Shigeru
S
D001692:Biological Transport; D018548:Cotyledon / Q000378:metabolism; D003624:Darkness; D018525:Germination; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D008027:Light; D009587:Nitrogen Isotopes; D018517:Plant Roots / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*; D013025:Glycine max / Q000502:physiology*
D009587:Nitrogen Isotopes; D011073:Polyamines; D011700:Putrescine