title: The effect of the 5-chloro-2-methylthio-6-(1-naphtyloxy)-1H-benzimidazole on the tegument of immature Fasciola hepatica in their natural host.
abstract: The damage to the tegument of 3-week-old Fasciola hepatica was evaluated by scanning electron microscopy (SEM) following treatment with the 5-chloro-2-methylthio-6-(1-naphtyloxy)-1H-benzimidazole (called compound alpha) in its natural host. For the present study, flukes were raised in pelibuey sheep infected orally with metacercariae of F. hepatica; the parasites were recovered from the liver of the sacrificed sheep after 6, 12 and 24 h of treatment with compound alpha. At 6 h of treatment, the flukes showed some lesions on the ventral surface of the anterior region, such as a swollen tegument and blebs. At 12 h after treatment, the specimens showed structural disorganization and spine loss in the ventral anterior region. The tegument of the flukes treated for 24 h was completely lost in some areas of the ventral surface, leaving an exposed basal lamina. The tegument of immature F. hepatica might be a target organ for compound alpha to exert its fasciolicide effect.
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de Mexico, 04510, Mexico City, Mexico.
authors.forename: Norma
authors.initials: N
authors.lastname: Rivera
authors.forename: Froylán
authors.initials: F
authors.lastname: Ibarra
authors.forename: Armando
authors.initials: A
authors.lastname: Zepeda
authors.forename: Teresa
authors.initials: T
authors.lastname: Fortoul
authors.forename: Germinal
authors.initials: G
authors.lastname: Cantó
authors.forename: Alicia
authors.initials: A
authors.lastname: Hernández
authors.forename: Rafael
authors.initials: R
authors.lastname: Castillo
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004817:Epidermis / Q000187:drug effects* / Q000648:ultrastructure; D005210:Fasciola hepatica / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D005211:Fascioliasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary; D007093:Imidazoles / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008855:Microscopy, Electron, Scanning; D009281:Naphthalenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology*; D013997:Time Factors
chemical_list: C468032:5-chloro-2-methylthio-6-(1-naphthyloxy)-1H-benzimidazole; D000871:Anthelmintics; D007093:Imidazoles; D009281:Naphthalenes
references.citation: Vet Parasitol. 2004 Feb 26;120(1-2):65-74
references.pmid: 15019144
references.citation: Vet Parasitol. 1998 Jun 30;77(4):229-36
references.pmid: 9763313
references.citation: Vet J. 1999 Sep;158(2):81-112
references.pmid: 10489266
references.citation: Chem Pharm Bull (Tokyo). 2002 May;50(5):649-52
references.pmid: 12036021
references.citation: Exp Parasitol. 1987 Feb;63(1):49-57
references.pmid: 3542548
references.citation: Parasitology. 1994 Jun;108 ( Pt 5):555-67
references.pmid: 8052511
references.citation: Parasitol Res. 1987;73(2):99-106
references.pmid: 3575297
references.citation: Parasitol Res. 2004 Jul;93(4):283-6
references.pmid: 15160287
references.citation: Parasitol Res. 2003 Oct;91(3):238-50
references.pmid: 12937959