Production and evaluation of a monoclonal antibody to Clostridium perfringens type D epsilon toxin.
A monoclonal antibody (mAb) against C. perfringens type D epsilon toxin was produced in hybridoma tissue culture supernatant and in BALB/C mice ascitic fluid. The mAb reacted with single band of the purified epsilon toxin and efficiently neutralized the lethal effect of epsilon toxin in mice. A competitive ELISA (C-ELISA) was developed using this mAb and used to evaluate C. perfringens type D vaccines. Individual serum samples from vaccinated rabbits were tested using C-ELISA and mouse neutralization test (MNT). The mAb based C-ELISA correlated with the conventional MNT for estimation of antitoxin level.
El-Enbaawy
M I
MI
Microbiology Department, Faculty of Veterinary Medicine, Cairo University Abbasia, Cairo, Egypt.
Abdalla
Y A
YA
Hussein
A Z
AZ
Osman
Roukaya M
RM
Selim
S A
SA
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000276:immunology* / Q000633:toxicity; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology; D003016:Clostridium perfringens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011817:Rabbits
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; C039811:Clostridium perfringens epsilon-toxin