title: Cupin-type phosphoglucose isomerases (Cupin-PGIs) constitute a novel metal-dependent PGI family representing a convergent line of PGI evolution.
abstract: Cupin-type phosphoglucose isomerases (cPGIs) were identified in some archaeal and bacterial genomes and the respective coding function of cpgi's from the euryarchaeota Archaeoglobus fulgidus and Methanosarcina mazei, as well as the bacteria Salmonella enterica serovar Typhimurium and Ensifer meliloti, was proven by functional overexpression. These cPGIs and the cPGIs from Pyrococcus and Thermococcus spp. represent the cPGI family and were compared with respect to kinetic, inhibitory, thermophilic, and metal-binding properties. cPGIs showed a high specificity for the substrates fructose-6-phosphate and glucose-6-phosphate and were inhibited by millimolar concentrations of sorbitol-6-phosphate, erythrose-4-phosphate, and 6-phosphogluconate. Treatment of cPGIs with EDTA resulted in a complete loss of catalytic activity, which could be regained by the addition of some divalent cations, most effectively by Fe2+ and Ni2+, indicating a metal dependence of cPGI activity. The motifs TX3PX3GXEX3TXGHXHX6-11EXY and PPX3HX3N were deduced as the two signature patterns of the novel cPGI family. Phylogenetic analysis suggests lateral gene transfer for the bacterial cPGIs from euryarchaeota.
authors.affiliation: Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität Kiel, Am Botanischen Garten 1-9, D-24118 Kiel, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hansen
authors.forename: Bettina
authors.initials: B
authors.lastname: Schlichting
authors.forename: Martina
authors.initials: M
authors.lastname: Felgendreher
authors.forename: Peter
authors.initials: P
authors.lastname: Schönheit
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D019605:Euryarchaeota / Q000201:enzymology* / Q000235:genetics; D019143:Evolution, Molecular; D005956:Glucose-6-Phosphate Isomerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013696:Temperature
chemical_list: D008670:Metals; D005956:Glucose-6-Phosphate Isomerase
references.citation: Eur J Biochem. 2001 Dec;268(24):6625-36
references.pmid: 11737217
references.citation: Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):453-65
references.pmid: 12595702
references.citation: Genome Res. 2002 Apr;12(4):532-42
references.pmid: 11932238
references.citation: Biochemistry. 2002 Jun 18;41(24):7659-69
references.pmid: 12056897
references.citation: Biochemistry. 2000 Feb 8;39(5):955-64
references.pmid: 10653639
references.citation: Microbiol Mol Biol Rev. 2000 Mar;64(1):153-79
references.pmid: 10704478
references.citation: Arch Microbiol. 2000 Feb;173(2):103-9
references.pmid: 10795681
references.citation: Nat Struct Biol. 2000 Jul;7(7):542-6
references.pmid: 10876237
references.citation: J Biol Chem. 2000 Aug 11;275(32):24608-12
references.pmid: 10827167
references.citation: J Biol Chem. 2000 Jul 28;275(30):23154-60
references.pmid: 10770936
references.citation: Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1111-3
references.pmid: 12777791
references.citation: J Biol Chem. 2003 Aug 29;278(35):33290-7
references.pmid: 12796486
references.citation: Biochem J. 2003 Oct 15;375(Pt 2):231-46
references.pmid: 12921536
references.citation: J Biol Chem. 2003 Nov 21;278(47):47261-8
references.pmid: 12970347
references.citation: Phytochemistry. 2004 Jan;65(1):7-17
references.pmid: 14697267
references.citation: Arch Microbiol. 2004 Jan;181(1):82-7
references.pmid: 14655001
references.citation: J Biol Chem. 2004 Jan 16;279(3):2262-72
references.pmid: 14551194
references.citation: Eur J Biochem. 2004 Mar;271(6):1106-16
references.pmid: 15009189
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7
references.pmid: 271968
references.citation: Arch Microbiol. 1980 Oct;127(3):289-98
references.pmid: 7004378
references.citation: EMBO J. 1990 Jan;9(1):9-15
references.pmid: 2295315
references.citation: J Bacteriol. 1991 May;173(10):3215-23
references.pmid: 1708765
references.citation: Comput Appl Biosci. 1993 Apr;9(2):197-9
references.pmid: 8481823
references.citation: Nat Struct Biol. 1996 May;3(5):470-9
references.pmid: 8612079
references.citation: J Mol Evol. 1996 Nov;43(5):453-9
references.pmid: 8875859
references.citation: Nature. 1997 Jun 19;387(6635):827-30
references.pmid: 9194566
references.citation: J Mol Biol. 1997 Oct 17;273(1):226-37
references.pmid: 9367758
references.citation: Nature. 1997 Nov 27;390(6658):364-70
references.pmid: 9389475
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: Microb Comp Genomics. 1998;3(2):141-8
references.pmid: 9697098
references.citation: Extremophiles. 2004 Dec;8(6):507-12
references.pmid: 15290326
references.citation: Nat Struct Biol. 2000 Nov;7(11):1036-40
references.pmid: 11062559
references.citation: FEMS Microbiol Lett. 2001 Jan 15;194(2):221-7
references.pmid: 11164312
references.citation: Biochemistry. 2001 Feb 13;40(6):1560-6
references.pmid: 11327814
references.citation: J Bacteriol. 2001 Jun;183(11):3428-35
references.pmid: 11344151
references.citation: J Mol Biol. 2001 Jun 1;309(2):447-63
references.pmid: 11371164
references.citation: Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):829-39
references.pmid: 11375502
references.citation: Biochemistry. 2001 Jul 3;40(26):7799-805
references.pmid: 11425306
references.citation: Science. 2001 Jul 27;293(5530):668-72
references.pmid: 11474104
references.citation: Nature. 2001 Oct 25;413(6858):852-6
references.pmid: 11677609
references.citation: J Biol Chem. 2001 Nov 2;276(44):40926-32
references.pmid: 11533028
references.citation: Cell Mol Life Sci. 2001 Oct;58(11):1650-65
references.pmid: 11706991
references.citation: J Mol Microbiol Biotechnol. 2002 Jul;4(4):453-61
references.pmid: 12125824
references.citation: J Mol Biol. 2002 Oct 11;323(1):77-84
references.pmid: 12368100
references.citation: Proteins. 2002 Dec 1;49(4):577-9
references.pmid: 12402366
references.citation: FEMS Microbiol Lett. 2002 Nov 5;216(2):249-53
references.pmid: 12435510
references.citation: FEBS Lett. 2003 Jan 30;535(1-3):200-4
references.pmid: 12560104
references.citation: Biochim Biophys Acta. 2003 Feb 21;1645(2):117-22
references.pmid: 12573240
references.citation: Trends Biochem Sci. 2001 Dec;26(12):740-6
references.pmid: 11738598