A Vibrio vulnificus type IV pilin contributes to biofilm formation, adherence to epithelial cells, and virulence.
Vibrio vulnificus expresses a multitude of cell-associated and secreted factors that potentially contribute to pathogenicity, although the specific roles of most of these factors have been difficult to define. Previously we have shown that a mutation in pilD (originally designated vvpD), which encodes a type IV prepilin peptidase/N-methyltransferase, abolishes expression of surface pili, suggesting that they belong to the type IV class. In addition, a pilD mutant exhibits reduced adherence to HEp-2 cells, a block in secretion of several exoenzymes that follow the type II secretion pathway, and decreased virulence. In this study, we have cloned and characterized a V. vulnificus type IV pilin (PilA) that shares extensive homology to group A type IV pilins expressed by many pathogens, including Vibrio cholerae (PilA), Pseudomonas aeruginosa (PilA), and Aeromonas hydrophila (TapA). The V. vulnificus pilA gene is part of an operon and is clustered with three other pilus biogenesis genes, pilBCD. Inactivation of pilA reduces the ability of V. vulnificus to form biofilms and significantly decreases adherence to HEp-2 cells and virulence in iron dextran-treated mice. Southern blot analysis demonstrates the widespread presence of both pilA and pilD in clinical as well as environmental strains of V. vulnificus.
Paranjpye
Rohinee N
RN
Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric, Seattle, WA 98112, USA.
Strom
Mark S
MS
D000595:Amino Acid Sequence; D000818:Animals; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009876:Operon; D010125:Ostreidae / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012758:Shellfish / Q000382:microbiology; D014735:Vibrio Infections / Q000382:microbiology; D041261:Vibrio vulnificus / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence
D036781:Fimbriae Proteins
Mol Microbiol. 1998 Sep;29(6):1481-92
9781884
Mol Microbiol. 1998 Oct;30(2):295-304
9791175
Infect Immun. 1998 Dec;66(12):5659-68
9826339
Infect Immun. 1999 Mar;67(3):1393-404
10024587
J Bacteriol. 1999 Jun;181(11):3606-9
10348878
Infect Immun. 1999 Oct;67(10):5447-54
10496928
Appl Environ Microbiol. 1987 Jan;53(1):193-5
3827248
Vaccine. 1987 Dec;5(4):283-8
2448968
Infect Immun. 1988 Jun;56(6):1641-6
2897336
J Exp Med. 1988 Sep 1;168(3):983-1002
2902184
J Infect Dis. 1990 Mar;161(3):541-8
1968936
Infect Immun. 1990 Jun;58(6):1769-73
2160432
Methods Enzymol. 1999;310:91-109
10547784
Mol Microbiol. 1999 Nov;34(3):586-95
10564499
Microbes Infect. 2000 Feb;2(2):177-88
10742690
Infect Immun. 2000 Jun;68(6):3569-73
10816513
J Mol Biol. 2000 Jun 16;299(4):1005-17
10843854
J Bacteriol. 2000 Jun;182(12):3405-15
10852871
Infect Immun. 2000 Sep;68(9):5096-106
10948131
J Bacteriol. 2001 Jan;183(2):758-62
11133972
Cell Microbiol. 2001 Mar;3(3):133-43
11260136
Infect Immun. 2001 Sep;69(9):5943-8
11500479
Infect Immun. 2001 Dec;69(12):7695-702
11705950
Dis Aquat Organ. 2002 Aug 15;51(1):13-25
12240967
Mol Microbiol. 2003 Jun;48(6):1511-24
12791135
Mol Microbiol. 2003 Jun;48(6):1647-64
12791145
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8508-13
12826608
Infect Immun. 2003 Oct;71(10):5461-71
14500463
Appl Environ Microbiol. 2003 Oct;69(10):6114-20
14532069
Genome Res. 2003 Dec;13(12):2577-87
14656965
J Bacteriol. 2004 Feb;186(3):889-93
14729720
Nature. 1970 Aug 15;227(5259):680-5
5432063
J Exp Med. 1973 Mar 1;137(3):571-89
4631989
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52
377280
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4
388439
Infect Immun. 1980 Sep;29(3):1146-51
6107276
J Infect Dis. 1981 Apr;143(4):525-32
6113259
Infect Immun. 1981 Nov;34(2):503-7
7309236
Infect Immun. 1984 Apr;44(1):38-40
6142863
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3471-5
6374658
EMBO J. 1982;1(8):945-51
6329717
Infect Immun. 1984 Aug;45(2):458-63
6746100
Gene. 1984 Sep;29(3):303-13
6237955
Infect Immun. 1985 Feb;47(2):446-51
2578434
J Bacteriol. 1986 Feb;165(2):367-72
2867989
J Mol Biol. 1986 May 5;189(1):113-30
3537305
Appl Environ Microbiol. 2003 Sep;69(9):5711-5
12957968
J Bacteriol. 1990 Jun;172(6):2911-9
1971619
Infect Immun. 1990 Aug;58(8):2706-9
2370116
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Infect Immun. 1991 Jan;59(1):192-7
1846124
J Biol Chem. 1991 Jan 25;266(3):1656-64
1671038
J Bacteriol. 1991 Feb;173(3):1175-80
1671384
Infect Immun. 1991 Mar;59(3):822-8
1671777
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3281-5
1901657
Gene. 1991 Mar 15;99(2):255-9
2022336
J Mol Biol. 1991 May 5;219(1):37-44
2023259
Mol Microbiol. 1992 May;6(10):1351-61
1640836
Infect Immun. 1993 Apr;61(4):1371-7
7681046
Annu Rev Microbiol. 1993;47:565-96
7903032
Gene. 1993 Dec 22;136(1-2):271-5
8294016
Gene. 1994 Dec 2;150(1):9-15
7959070
J Bacteriol. 1995 Sep;177(18):5294-302
7665518
Appl Environ Microbiol. 1996 Feb;62(2):717-24
8593075
Infect Immun. 1996 Jul;64(7):2834-8
8698519
Diagn Microbiol Infect Dis. 1996 Mar;24(3):165-7
8724403
Mol Microbiol. 1996 Feb;19(4):857-69
8820654
Gene. 1997 Jun 11;192(1):99-108
9224879
Mol Microbiol. 1997 Jul;25(2):319-28
9282744
Science. 1998 Jun 26;280(5372):2114-8
9641917