title: Extractable bone morphogenetic protein and correlation with induced new bone formation in an in vivo assay in the athymic mouse model.
abstract: A correlation between extractable bone morphogenetic proteins (BMPs) in demineralized bone matrix (DBM) and osteoinduction has been suggested. Extractable BMP-4 and osteoinductivity of DBM from 40 donors were assessed using enzyme-linked immunosorbent assay (ELISA) and in vivo athymic mouse assay, respectively. Extractable BMP-4 level averaged 3.7 +/- 0.21 ng/g of DBM and correlated with osteoinductivity of the DBM in an in vivo assessment of induced newbone formation.
authors.affiliation: LifeNet, 5809 Ward Court, Virginia Beach, VA 23455, USA.
authors.forename: Sittisak
authors.initials: S
authors.lastname: Honsawek
authors.forename: Ralph M
authors.initials: RM
authors.lastname: Powers
authors.forename: Lloyd
authors.initials: L
authors.lastname: Wolfinbarger
mesh_terms: D000818:Animals; D001681:Biological Assay; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000737:chemistry; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000302:isolation & purification* / Q000502:physiology; D016025:Bone Transplantation; D002118:Calcium / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis / Q000502:physiology*; D010316:Particle Size; D014183:Transplantation, Heterologous
chemical_list: C525809:BMP4 protein, human; C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D002118:Calcium