Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxin B1.
Preparation of an antibody-colloidal gold probe (conjugate) specific to aflatoxin B1 (AFB1) and its use in developing a rapid AFB1 diagnostic method was presented in this paper. Monodispersional nanogold colloid was synthesized and preparation of nanogold-labeled polyclonal antibody probe to aflatoxin B1 under friendly and optimal condition. Combination of antibody with nanogold particles was also characterized by UV-visible (UV-vis) light absorption spectra, transmission electron microscopy (TEM), fluorescence spectroscopy, titers, cross reactivity and stability measurements. Furthermore, nanogold-labeled probe was used to develop an immunochromatographic (IC) method for aflatoxin B1 analysis. With this method, analysis could be completed in less than 10 min. Detection time was reduced 6-10 times comparative with ELISA. With visual observation, lower test limit was found to be around 2.5 ng/ml aflatoxin B1 standard solution, which was increased to two times of ELISA.
Xiulan
Sun
S
School of Food Science, Southern Yangtze University, 170, Huihe Road, Wuxi, Jiangsu 214036, PR China. sxlzzz@yahoo.com.cn
Xiaolian
Zhao
Z
Jian
Tang
T
Zhou
Jun
J
Chu
F S
FS
D016604:Aflatoxin B1 / Q000276:immunology / Q000302:isolation & purification*; D000907:Antibodies, Bacterial / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D003102:Colloids; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006046:Gold; D007159:Immunologic Tests / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D000907:Antibodies, Bacterial; D003102:Colloids; D006046:Gold; D016604:Aflatoxin B1