Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9.
Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5alpha. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.
Huang
Chien-Jui
CJ
Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan, Republic of China. cychen@ntu.edu.tw.
Wang
Tang-Kai
TK
Chung
Shu-Chun
SC
Chen
Chao-Ying
CY
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D002688:Chitinases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D027762:Lilium / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D013624:Taiwan
D000935:Antifungal Agents; D002688:Chitinases