title: Brominated flame retardants: activities in a crustacean development test and in an ecdysteroid screening assay.
abstract: Brominated flame retardants (BFRs) were investigated for toxic effects both in vivo and in vitro in two invertebrate bioassays. Subchronic effects of tetrabromobisphenol A (TBBPA), tribromophenol (TBP), and four polybrominated diphenyl ethers ([PBDEs]; BDE-28, BDE-47, BDE-99, and BDE-100) on larval development of the marine copepod Acartia tonsa were studied. For TBBPA and TBP 5-d effective median concentration (EC50) values for inhibition of the larval development rate were 125 and 810 microg/L, respectively, whereas the PBDEs were much more potent with 5-d EC50 in the low microg/L range (1.2 microg/L for BDE-100; 4.2 microg/L for BDE-99; 13 microg/L for BDE-28; and 13 microg/L for BDE-47). These concentrations were up to two orders of magnitude below the 48-h LC50 for acute adult toxicity (108 microg/L for BDE-28; 400 microg/L for TBBPA; 520 microg/L for BDE-100; 705 microg/L for BDE-99; 1,500 microg/L for TBP; and 2,370 microg/L for BDE-47). To distinguish between general toxicological and endocrine-mediated toxic effects, the BFRs were assessed in vitro for ecdysteroid agonistic/antagonistic activity with the ecdysteroid-responsive Drosophila melanogaster B(II)-cell line. The pentabrominated diphenyl ethers BDE-99 and BDE-100 showed weak ecdysteroid antagonistic activity. Thus, these PBDEs may be regarded as potential endocrine disrupters in invertebrates. The combination of in vitro assays and subchronic biotests with ecologically important crustacean species is a rapid and cost-effective tool when screening for sublethal effects of BFRs and other chemicals.
authors.affiliation: Environment & Resources, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark. lew@er.dtu.dk
authors.forename: Leah
authors.initials: L
authors.lastname: Wollenberger
authors.forename: Laurence
authors.initials: L
authors.lastname: Dinan
authors.forename: Magnus
authors.initials: M
authors.lastname: Breitholtz
mesh_terms: D000818:Animals; D002460:Cell Line; D003445:Crustacea / Q000187:drug effects* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology; D004441:Ecdysterone / Q000819:agonists / Q000032:analysis* / Q000037:antagonists & inhibitors; D005411:Flame Retardants / Q000633:toxicity*; D006842:Hydrocarbons, Brominated / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D007814:Larva / Q000187:drug effects* / Q000378:metabolism; D010647:Phenyl Ethers / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D011075:Polybrominated Biphenyls / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D018570:Risk Assessment; D014018:Tissue Distribution
chemical_list: D005411:Flame Retardants; D006842:Hydrocarbons, Brominated; D010647:Phenyl Ethers; D011075:Polybrominated Biphenyls; D004441:Ecdysterone; C020806:tetrabromobisphenol A