title: [Determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC].
abstract: OBJECTIVE
  To establish methods for the determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC.
  
  
  METHODS
  The determination of phillyrin was carried out on YWG-C18 column (4.6 mm x 250 mm, 10 microm), using acetonitrile-water (25:75) as mobile phase at a flow rate of 0.8 mL x min(-1) and detected at the wavelength 277 nm. The determination of forsythoside A was carried out with YWG-C18 column(4.6 mm x 250 mm,10 microm), using acetonitrile-water-Acetic acid (17:83:0.4) as mobile phase at a flow rate of 1.0 mL x min(-1) and detected at the wavelength 280 nm.
  
  
  RESULTS
  The average recovery of phillyrin was 99.6%, RSD = 1.9% (n = 5). The average recovery of forsythoside A was 101.3%, RSD = 2.5% ( n = 5).
  
  
  CONCLUSIONS
  The methods were simple and accurate and could be used to control the quality of the Lianqiao Bingdu Qing capsule.
authors.affiliation: Capital University of Medical Sciences, Beijing 100054, China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Zhao
authors.forename: Ren-Bing
authors.initials: RB
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Lan-Zhen
authors.initials: LZ
authors.lastname: Zhang
mesh_terms: D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D031657:Forsythia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; C058049:forsythiaside; C075528:phillyrin