Increased cyclin E expression may obviate the role of cyclin D1 during brain development in cyclin D1 knockout mice.
Cyclins D and E play critical roles during the G1 phase of mammalian cell division. Cyclin D1 expression is high and expected to play an important role during mouse brain development. However, in the present study, we found no difference in CNS morphology between cyclin D1 knockout (KO) and control wild-type mice at the ages of 1, 4 and 12 months. Analysis of protein expression in embryonic brains revealed that cyclin E is obviously increased in cyclin D1 KO mice at 13.5 days post coitum. At the same age a high level of cyclin D1 expression is detected in the embryonic brain of wild-type mice. The data indicate that enhanced cyclin E protein expression in cyclin D1 KO mice may obviate the role of cyclin D1 and contribute to the normal brain development of cyclin D1 KO mice.
Chen
Zhiguo
Z
Division of Experimental Geriatrics, Department of Neurotec, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden.
Duan
Rui-Sheng
RS
Zhu
Yu
Y
Folkesson
Ronnie
R
Albanese
Chris
C
Winblad
Bengt
B
Zhu
Jie
J
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D019938:Cyclin D1 / Q000235:genetics / Q000502:physiology*; D056742:Cyclin D2; D056743:Cyclin D3; D019927:Cyclin E / Q000378:metabolism*; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005838:Genotype; D047468:Immunoprecipitation / Q000379:methods; D051379:Mice; D018345:Mice, Knockout / Q000502:physiology
C486196:Ccnd2 protein, mouse; C534697:Ccnd3 protein, mouse; D056742:Cyclin D2; D056743:Cyclin D3; D019927:Cyclin E; D016213:Cyclins; D019938:Cyclin D1