[DMDUse of fetal specific antibody-HbF to detect fetal erythroblasts for non-invasive prenatal diagnosis of DMD].
Maternal blood was obtained at 8-26 weeks of gestation. After discontinuous density gradient centrifugation with Percoll, HbF antibody was used to identify fetal NRBC. The precise differentiation between fetal and maternal NRBC is based on the constitutional difference between fetal and adult hemoglobin (Hb). Fetal cells appear yellow cytoplasmic staining,while adult cells colorless. NRBCs were collected by micromanipulation and whole genome amplification was performed. DMD was prenatally diagnosed by using the combination of sex determination,multiplex PCR and linkage analysis of several STR sites of dystrophin. NRBCs stained with HbF were found in all of 20 maternal blood samples with gestations, and 7 fetuses with risk of DMD were diagnosed. We concluded that HbF antibody could identify fetal NRBC efficaciously, and this is a kind of more prospective application method.
Liu
Li-Ying
LY
Department of Medical Genetics, China Medical University, Shenyang, 110001, China. jjljcl@mail.sy.ln.cn
Jin
Chun-Lian
CL
Lin
Chang-Kun
CK
Wu
Ying-Yu
YY
Sun
Kai-Lai
KL
D000906:Antibodies / Q000032:analysis; D041321:Chromosomes, Human, X; D016189:Dystrophin / Q000235:genetics; D004900:Erythroblasts / Q000473:pathology*; D005091:Exons; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005319:Fetal Hemoglobin / Q000032:analysis / Q000276:immunology*; D017353:Gene Deletion; D006801:Humans; D007438:Introns; D008297:Male; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis* / Q000235:genetics; D011247:Pregnancy / Q000097:blood; D011296:Prenatal Diagnosis / Q000379:methods*; D012732:Sex Determination Analysis
D000906:Antibodies; D016189:Dystrophin; D005319:Fetal Hemoglobin