Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation.
Recent studies suggest that neutrophils may play a role in antigen presentation. In support of this hypothesis it has been shown that these cells appear to contain cytoplasmic stores of molecules required for this function, i.e. major histocompatibility complex class II (DR) antigen, CD80 and CD86. In this study we have considered a mechanism for the translocation of these preformed molecules onto the cell surface which does not require active synthesis. Cross-linking of the Mac-1 molecule (CD18 + CD11b) was shown to result in rapid cell surface expression of CD80, CD86 and DR antigen on the surface of normal human peripheral blood neutrophils. A distinct subpopulation (approximately 20%) of neutrophils appeared to be enlarged and were found to express significantly elevated levels of these molecules on the cell surface following cross-linking of CD11b when compared with control cells. The level of expression of CD80, CD86 and DR antigen on these large cells was comparable to, and in some cases greater than, the levels found expressed on the surface of monocytes obtained from the same donors. In addition, these cytoplasmic molecules were shown by confocal laser microscopy and by immunoelectron microscopy to be located within secretory vesicles. Following rapid translocation onto the cell surface, CD80 and CD86 appeared to be colocalized within large clusters reminiscent of the supramolecular antigen clusters previously found on conventional antigen-presenting cells. These findings therefore lend further support for the hypothesis that neutrophils may have a role to play in antigen presentation and/or T-cell activation.
Sandilands
Gavin P
GP
University Department of Pathology, Western Infirmary, Glasgow, UK. gps1z@clinmed.gla.ac.uk
Ahmed
Zubir
Z
Perry
Nicole
N
Davison
Martin
M
Lupton
Alison
A
Young
Barbara
B
D017951:Antigen Presentation / Q000276:immunology*; D015703:Antigens, CD / Q000097:blood; D018122:B7-1 Antigen / Q000097:blood; D051940:B7-2 Antigen; D039481:CD11b Antigen / Q000097:blood*; D003593:Cytoplasm / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000097:blood; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D009504:Neutrophils / Q000276:immunology* / Q000648:ultrastructure; D022142:Secretory Vesicles / Q000276:immunology / Q000648:ultrastructure; D014178:Translocation, Genetic / Q000276:immunology
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D039481:CD11b Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins
Blood. 1997 Jun 1;89(11):4128-35
9166855
Physiol Rev. 2003 Apr;83(2):581-632
12663867
J Leukoc Biol. 1997 Sep;62(3):356-62
9307074
Infect Immun. 1997 Nov;65(11):4747-53
9353060
Blood. 1999 Feb 15;93(4):1413-21
9949186
J Immunol. 1999 Nov 15;163(10):5592-8
10553088
J Immunol Methods. 1999 Dec 17;232(1-2):153-68
10618517
J Immunol. 2000 Apr 15;164(8):4257-64
10754323
Cell Biol Int. 2000;24(3):163-74
10772777
Cell Biochem Funct. 2000 Jun;18(2):127-32
10814971
Int J Hematol. 2000 Jul;72(1):48-54
10979209
Immunology. 2000 Dec;101(4):521-30
11122456
Annu Rev Immunol. 2001;19:225-52
11244036
Annu Rev Immunol. 2001;19:375-96
11244041
J Immunol. 2003 Aug 1;171(3):1128-32
12874197
Trends Immunol. 2003 Sep;24(9):485-90
12967672
Arthritis Rheum. 2003 Oct;48(10):2796-806
14558085
Microbes Infect. 2003 Nov;5(14):1317-27
14613775
Nat Cell Biol. 2004 Aug;6(8):709-20
15258588
J Trauma. 2004 Aug;57(2):347-51
15345984
J Lab Clin Med. 1984 Nov;104(5):698-710
6208302
J Clin Invest. 1987 Aug;80(2):535-44
3038962
Am J Physiol. 1991 Dec;261(6 Pt 1):C1154-61
1767817
J Immunol. 1993 Aug 1;151(3):1482-90
8335942
Biochem J. 1994 Apr 15;299 ( Pt 2):473-9
8172608
Am J Physiol. 1994 Dec;267(6 Pt 1):C1623-32
7810604
Immunol Today. 1995 Jan;16(1):21-6
7880385
Immunity. 1994 Dec;1(9):793-801
7534620
J Immunol. 1995 Apr 15;154(8):4157-65
7535822
J Immunol. 1995 Dec 15;155(12):5750-9
7499863
Cell Immunol. 1996 Jul 10;171(1):120-5
8660846
Blood. 1997 May 15;89(10):3503-21
9160655
Trends Immunol. 2001 Apr;22(4):187-91
11274922
J Immunol. 2001 Apr 15;166(8):5201-7
11290804
Kidney Int. 2001 Dec;60(6):2247-62
11737598
Immunology. 2003 Mar;108(3):329-37
12603599
J Cell Sci. 1997 Jul;110 ( Pt 13):1453-63
9224763