title: Agglutination study of poly(allyl-alpha-D-glucopyranose/styrene) latex particles in the presence of Concanavalin A.
abstract: Latex agglutination of the glucose-modified latex, which were synthesized by emulsion copolymerization of allyl-modified glucose and styrene monomers by specific binding interactions between Concanavalin A (Con A) and allyl-alpha-d-glucopyranose, were investigated. The surface of the glucose-modified latex was characterized by dye-partition method and the number of glucose was 1517 per latex particle. The average particle size and the polydispersity index of the latex were 78.3 and 1.005, respectively. Time-evolution adsorption behavior of various concentrations of the Con A and the consequent latex agglutination were studied by UV spectrophotometer at 540 nm and zeta-potential analyzer at the fixed latex concentration of 0.02 wt%. Specific binding between Con A and allyl-alpha-D-glucopyranose leaded the latex particles to coagulate by decreasing the electrostatic repulsion between the particles and mobility.
authors.affiliation: Nanosphere Process and Technology Laboratory, Department of Chemical Engineering, Yonsei University, 134 Shinchon-Dong, Seodaemoon-Ku, Seoul 120-749, South Korea.
authors.forename: Jung Yul
authors.initials: JY
authors.lastname: Lee
authors.forename: In Woo
authors.initials: IW
authors.lastname: Cheong
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000371:Agglutination; D003208:Concanavalin A / Q000737:chemistry*; D004355:Drug Stability; D005947:Glucose; D005960:Glucosides / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008968:Molecular Conformation; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D005960:Glucosides; D011137:Polystyrenes; C503400:poly(allyl glucopyranose-styrene); D003208:Concanavalin A; D005947:Glucose
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