The core lipopolysaccharide of Escherichia coli is a ligand for the dendritic-cell-specific intercellular adhesion molecule nonintegrin CD209 receptor.
The dendritic-cell-specific intercellular adhesion molecule nonintegrin (DC-SIGN) CD209 is a receptor for Escherichia coli K-12 that promotes bacterial adherence and phagocytosis. However, the ligand of E. coli for DC-SIGN has not yet been identified. In this study, we found that DC-SIGN did not mediate the phagocytosis of several pathogenic strains of E. coli, including enteropathogenic E. coli, enterohemorrhagic E. coli, enterotoxigenic E. coli, and uropathogenic E. coli, in dendritic cells or HeLa cells expressing human DC-SIGN antigen. However, we showed that an outer core lipopolysaccharide (LPS) (rough) mutant, unlike an inner core LPS (deep rough) mutant or O-antigen-expressing recombinant of E. coli K-12 was phagocytosed. These results demonstrate that the host cells expressing DC-SIGN can phagocytose E. coli in part by interacting with the complete core region of the LPS molecule. These results provide a mechanism for how O antigen acts as an antiphagocytic factor.
Klena
John
J
School of Molecular Biosciences, Washington State University, Pullman, Washington, USA.
Zhang
Pei
P
Schwartz
Olivier
O
Hull
Sheila
S
Chen
Tie
T
D001422:Bacterial Adhesion / Q000502:physiology; D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry / Q000502:physiology*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000502:physiology; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000502:physiology*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
Cell. 2000 Mar 3;100(5):587-97
10721995
J Bacteriol. 1992 Apr;174(8):2525-38
1348243
Infect Immun. 2001 Nov;69(11):6923-30
11598067
Immunity. 2002 Jan;16(1):145-55
11825573
Infect Immun. 2002 May;70(5):2583-90
11953399
Blood. 2002 Sep 1;100(5):1780-6
12176900
J Bacteriol. 1992 Jul;174(14):4736-45
1624461
J Bacteriol. 1992 Jul;174(14):4746-52
1624462
J Bacteriol. 1992 Nov;174(22):7297-307
1385388
Mol Microbiol. 1993 Jul;9(2):393-402
7692219
Microbiol Rev. 1993 Sep;57(3):655-82
7504166
J Immunol. 1995 Apr 15;154(8):3821-35
7706722
Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2588-92
8637918
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14851-6
8962144
J Exp Med. 1997 May 5;185(9):1557-64
9151893
Infect Immun. 1998 Sep;66(9):4244-53
9712774
Mol Microbiol. 1998 Oct;30(2):221-32
9791168
Microbes Infect. 2002 Sep;4(11):1125-32
12361912
J Biol Chem. 2002 Nov 1;277(44):41613-23
12196537
J Exp Med. 2003 Jan 6;197(1):7-17
12515809
J Exp Med. 2003 Jan 6;197(1):121-7
12515819
J Biol Chem. 2003 Feb 21;278(8):5513-6
12496255
Mol Microbiol. 2003 Mar;47(5):1395-406
12603743
Microbiology. 2003 Apr;149(Pt 4):925-39
12686635
J Virol. 2003 May;77(9):5313-23
12692233
Science. 2003 May 23;300(5623):1295-7
12730499
J Immunol. 2003 Jul 15;171(2):669-77
12847232
Nature. 2003 Jul 17;424(6946):329-34
12867985
Nat Med. 2003 Aug;9(8):996-7
12894159
J Endotoxin Res. 2003;9(4):244-9
12935355
Nat Rev Immunol. 2003 Sep;3(9):697-709
12949494
FEMS Microbiol Lett. 2003 Sep 26;226(2):355-61
14553933
Mol Microbiol. 2004 Jan;51(1):215-25
14651623
Infect Immun. 2004 Sep;72(9):5080-8
15322001
FEMS Immunol Med Microbiol. 2004 Oct 1;42(2):261-6
15364113
Prog Food Nutr Sci. 1983;7(3-4):75-89
6140705
J Bacteriol. 1991 Oct;173(20):6428-31
1655711
J Bacteriol. 1992 Feb;174(3):930-4
1732225
J Biol Chem. 2001 May 18;276(20):17413-9
11278708