Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA.
Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method--Random Amplification of Polymorphic DNA (RAPD)--to identify genetic markers in B. anthracis strains. Twenty-five differential genetic markers were identified which divided the strains into five different groups. Three selected RAPD-markers were cloned and sequenced. The five RAPD-derived genotypes could be defined by integration of these three markers. This system offers a simple non-expensive method to classify B. anthracis strains in laboratories involved in the research of this bacterium.
Levy
Haim
H
Department of Infectious Diseases, Israel Institute for Biological Research, PO Box 19, Ness-Ziona, Israel. levy@iibr.gov.il
Fisher
Morly
M
Ariel
Naomi
N
Altboum
Zeev
Z
Kobiler
David
D
D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005819:Genetic Markers; D005838:Genotype; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D004269:DNA, Bacterial; D005819:Genetic Markers