title: Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin.
abstract: The Aeromonas sobria hemolysin causes diarrhea following infection by this enteropathogenic bacterium. We previously identified the putative receptor for A. sobria hemolysin as a p66 protein on Intestine 407 cells (Microb. Pathog. 27 (1999) 215-221). Here, we have partially purified and obtained a peptide mass fingerprint of p66 which revealed its identity with placental alkaline phosphatase (PLAP). Recombinant PLAP expressed in 293T cells was also found to bind to hemolysin and the binding was found not to be dependent on the N-linked glycosylation of PLAP. By immunohistochemical analysis, PLAP expression was detected in human intestinal mucosa, the target tissue in disease. In addition to PLAP, hemolysin also binds to intestinal alkaline phosphatase (IAP), an enzyme that is also abundantly expressed in intestine. Thus, both PLAP and IAP are very likely involved in the pathogenesis of diarrhea caused by this bacterial toxin.
authors.affiliation: Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Wada
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Wang
authors.forename: Hajime
authors.initials: H
authors.lastname: Isomoto
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Satomi
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takao
authors.forename: Akira
authors.initials: A
authors.lastname: Takahashi
authors.forename: Shizuka
authors.initials: S
authors.lastname: Awata
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Nomura
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Fujii
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
authors.forename: Keinosuke
authors.initials: K
authors.lastname: Okamoto
authors.forename: Joel
authors.initials: J
authors.lastname: Moss
authors.forename: José Luis
authors.initials: JL
authors.lastname: Millán
authors.forename: Toshiya
authors.initials: T
authors.lastname: Hirayama
mesh_terms: D000333:Aeromonas / Q000378:metabolism / Q000472:pathogenicity*; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D058851:GPI-Linked Proteins; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D006460:Hemolysin Proteins; D007527:Isoenzymes; C063088:ALPI protein, human; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental