Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections.
Evaluation of estrogen and progesterone receptors in breast cancer is widely used for the prediction of the response to endocrine therapy and as a biologic parameter closely related to disease prognosis. Immunohistochemistry is considered a specific, sensitive, and economic method for the determination of estrogen receptor/progesterone receptor status. The authors developed the first rabbit antiestrogen receptor monoclonal antibody (clone SP1) used in immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections especially from breast carcinomas. This new antibody, compared with currently available antiestrogen receptor antibodies, has important advantages, including its reactivity even without heat-based antigen retrieval of fixed, embedded tissue sections in immunohistochemistry, and the predominance of nuclear immunostaining with only a very low cytoplasmic signal. A comparative study of immunohistochemistry on 61 histologic specimens from breast cancer cases showed that SP1 yields the same results as the well-known, standardized mouse monoclonal antibody to estrogen receptor (clone 1D5). Antibody affinity of SP1 is 8 times higher than that of 1D5. Thus, SP1 may prove of great value in the assessment of estrogen receptor status in human breast cancer.
Huang
Zhida
Z
Spring Bioscience Corporation, Fremont, CA 94538, USA.
Zhu
Weimin
W
Szekeres
George
G
Xia
Haiying
H
D000818:Animals; D000911:Antibodies, Monoclonal; D005404:Fixatives; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding / Q000379:methods*; D011817:Rabbits; D011960:Receptors, Estrogen / Q000032:analysis*; D016707:Tissue Fixation / Q000379:methods*
D000911:Antibodies, Monoclonal; D005404:Fixatives; D011960:Receptors, Estrogen; D005557:Formaldehyde