Induction and characterization of streptomycin-resistant mutants in Capsicum praetermissum.
Streptomycin-resistant mutants were isolated from mutagenised cotyledon explants of Capsicum praetermissum Heiser & Smith. The explants were mutagenised with N-ethyl-N-nitrosourea, which resulted in a high frequency of streptomycin-resistant mutants (18.0%) and a low frequency of chlorophyll-deficient (albino) mutants (8.0%). Complete streptomycin-resistant plantlets were obtained after rooting of the regenerated green shoots on rooting medium containing 1.0 mg L-1 IAA and 500 mg L-1 streptomycin sulphate. Leaf-segment assay of these plantlets revealed that they were resistant to streptomycin but sensitive to chloramphenicol, kanamycin, lincomycin, and spectinomycin. Reciprocal crosses between streptomycin-resistant and -sensitive plants showed a non-Mendelian transmission of resistance by female parents.
Venkataiah
Peddaboina
P
Plant Biotechnology Laboratory, Department of Botany, Kakatiya University, Warangal-506 009 A P, India. peddaboina@hotmail.com
Christopher
Thamidala
T
Subhash
Karampuri
K
D002212:Capsicum / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics*; D009154:Mutation; D013307:Streptomycin / Q000494:pharmacology*
D013307:Streptomycin