Expression of adhesion molecules and RANTES in kidney transplant from nonheart-beating donors.
The main difference between cadaveric kidneys from donors with a heartbeat (HBD) and kidneys from nonheart-beating donors (NHBD) is related to warm ischemia/reperfusion time which constitutes an acute inflammatory process. On the contrary, brain death induces in HBD expression of pro-inflammatory adhesion molecules, making it important to evaluate this kind of molecules in both types of donors. Human renal biopsies from NHBD, HBD and normal kidneys (ischemia time = 0) were taken and frozen just before transplant. A semi-quantitative RT-PCR method was used to determine intracellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), lymphocyte function associated antigen (LFA-1), LFA-3, CD40, CD40 ligand (CD40L) and RANTES (regulated upon activation, normal T-cell expressed and secreted) gene expression. We have detected an elevated relative gene expression of ICAM-1, VCAM-1 and RANTES in NHBD biopsies compared with normal kidneys. In the case of RANTES, the gene expression from NHBD biopsies was higher than observed in HBD biopsies. The rest of genes were not augmented in any group. Preliminary data about early outcome of transplants indicates a correlation between pretransplant RANTES high gene expression levels and early post-transplant acute rejection. The gene expression of pro-inflammatory molecules like adhesion molecules and RANTES is augmented in kidneys from cadaveric NBD just before transplant. The expression is higher probably because of the prolonged warm ischemia period. A larger clinical study is necessary to clarify the effects of these variable expressions on the transplant outcome.
Gomez del Moral
Manuel
M
Department of Cell Biology, Faculty of Medicine, Complutense University, Avda. Complutense S/N 28040 Madrid, Spain.
Aviles
Beatriz
B
Colberger
Ingrid K
IK
Campos-Martin
Yolanda
Y
Suela
Javier
J
Alvarez
Joaquin
J
Perez-Contin
M Jesús
MJ
Sánchez-Fructuoso
Ana
A
Barrientos
Alberto
A
Martinez-Naves
Eduardo
E
D000328:Adult; D018946:Chemokine CCL5 / Q000235:genetics*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D016030:Kidney Transplantation; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014019:Tissue Donors; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics*
D018946:Chemokine CCL5; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1