Membrane sensors for the selective determination of thiopental.
Novel miniaturized polyurethane (PU) membrane sensors in an all-solid state graphite support were developed, electrochemically evaluated and used for the assay of thiopental drug. The thiopental (T) sensors are based on the formation of ion-association complexes of thiopental with copper(II) and cobalt(II)-bathophenanthroline (bphen) counter anions as electroactive materials dispersed in a polyurethane matrix. The sensors show a linear response for thiopental over the range of 1 x 10(-1) - 5 x 10(-5) M thiopental at 25 degrees C over the pH range 6 - 11 with anionic slopes of -28.7 and -28.3 mV decade(-1) with Cu- and Co-bphen thiopental membrane sensors, respectively. These sensors exhibit a fast response time (25 - 45 s), a low detection limit (5 x 10(-6) M), a long lifetime (7 weeks) and good stability. The selectivity coefficients for thiopental sensors relative to the number of interfering anions, were investigated. These sensors were used for the direct potentiometry of thiopental in a pharmaceutical formulation and human serum. Results with mean accuracy of 99.8 +/- 0.5% of nominal were obtained, which compare well with data obtained using spectrophotometric (UV-Vis) and British Pharmacopoeia (BP) methods.
Rizk
Nashwa M H
NM
Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Sadat City, Egypt.
Othman
Abdel-Hameed M
AH
D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis* / Q000097:blood / Q000737:chemistry*; D008567:Membranes, Artificial; D008904:Miniaturization; D015394:Molecular Structure; D011199:Potentiometry; D012680:Sensitivity and Specificity; D013874:Thiopental / Q000032:analysis* / Q000097:blood / Q000737:chemistry*
D006993:Hypnotics and Sedatives; D008567:Membranes, Artificial; D013874:Thiopental