Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin.
The Aeromonas sobria hemolysin causes diarrhea following infection by this enteropathogenic bacterium. We previously identified the putative receptor for A. sobria hemolysin as a p66 protein on Intestine 407 cells (Microb. Pathog. 27 (1999) 215-221). Here, we have partially purified and obtained a peptide mass fingerprint of p66 which revealed its identity with placental alkaline phosphatase (PLAP). Recombinant PLAP expressed in 293T cells was also found to bind to hemolysin and the binding was found not to be dependent on the N-linked glycosylation of PLAP. By immunohistochemical analysis, PLAP expression was detected in human intestinal mucosa, the target tissue in disease. In addition to PLAP, hemolysin also binds to intestinal alkaline phosphatase (IAP), an enzyme that is also abundantly expressed in intestine. Thus, both PLAP and IAP are very likely involved in the pathogenesis of diarrhea caused by this bacterial toxin.
Wada
Akihiro
A
Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki 852-8523, Japan.
Wang
Ai-Ping
AP
Isomoto
Hajime
H
Satomi
Yoshinori
Y
Takao
Toshifumi
T
Takahashi
Akira
A
Awata
Shizuka
S
Nomura
Tomohiko
T
Fujii
Yoshio
Y
Kohno
Shigeru
S
Okamoto
Keinosuke
K
Moss
Joel
J
Millán
José Luis
JL
Hirayama
Toshiya
T
D000333:Aeromonas / Q000378:metabolism / Q000472:pathogenicity*; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D058851:GPI-Linked Proteins; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism
D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D006460:Hemolysin Proteins; D007527:Isoenzymes; C063088:ALPI protein, human; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental