Protection of trocar sites from gallbladder cancer implantation in a murine model by physically covering the sites.
Laparoscopic surgery for malignant diseases is associated with a risk for port site recurrence (PSR), and this problem remains to be resolved. This study was performed to evaluate the role that Seprafilm and Interseed (TC7) play in the prevention of PSR, using a nude mouse model of PSR. Nude mice with 2-mm port sites were created. The resultant peritoneal defects were covered with either Seprafilm or Interseed (TC7). The protective effect of Seprafilm and Interseed (TC7) on GB-dl (a human gallbladder cancer cell line) implantation at port sites was assessed after 7 days. In addition, the effects of Seprafilm and Interseed (TC7) on wound healing at the port sites were also observed. The Seprafilm and Interseed (TC7) groups showed a significantly lower incidence of PSR than the control group. Both Seprafilm and Interseed (TC7) changed into a gel form and covered the ruptured muscle layer and injured peritoneum for 3-5 days. When the peritoneal defect, produced by trocar insertion, was physically covered with either Seprafilm or Interseed (TC7), the incidence of PSR was observed to significantly decrease. These materials seem to be promising modalities for the prevention of PSR.
Sasaki
Takamitsu
T
First Department of Surgery, Fukuoka University School of Medicine, Fukuoka, Japan. takamitu@fc4.so-net.ne.jp
Shimura
Hideo
H
Tanaka
Toshihiro
T
Nakashima
Keisuke
K
Matsuo
Katsuichi
K
Ikeda
Seiyo
S
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002483:Cellulose, Oxidized / Q000627:therapeutic use*; D005706:Gallbladder Neoplasms / Q000473:pathology / Q000601:surgery*; D005782:Gels; D006820:Hyaluronic Acid; D020007:Implants, Experimental; D010535:Laparoscopy; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008819:Mice, Nude; D009366:Neoplasm Seeding; D010534:Peritoneal Neoplasms / Q000209:etiology / Q000517:prevention & control*; D018709:Statistics, Nonparametric; D013525:Surgical Instruments; D014945:Wound Healing / Q000187:drug effects
D001672:Biocompatible Materials; D002483:Cellulose, Oxidized; D005782:Gels; C060002:INTERCEED; D008567:Membranes, Artificial; C118198:Seprafilm; D006820:Hyaluronic Acid