Serotonin transport kinetics correlated between human platelets and brain synaptosomes.
BACKGROUND
Blood platelets have been used extensively as a model system for investigating the role of the serotonin transporter (SERT) in various psychiatric disorders, especially depression. However, to date, it is not known whether platelet serotonin (5-HT) transport would be related to that in brain.


OBJECTIVE
We examined 5-HT transport kinetics simultaneously in human blood platelets and human cortical brain synaptosomes to determine whether they were correlated.


METHODS
Blood platelets and synaptosomes were obtained from 25 patients undergoing epileptic surgery. Synaptosomes were obtained from normal margins of surgical neuropathology specimens of anterotemporal cortex.


RESULTS
Platelet SERT V(max) was significantly correlated with brain SERT V(max) on linear regression (r=0.58, p<0.005), after controlling for the confounding effects of gender (t=-2.4, p=0.025) and time of day (t=2.1, p<0.05). Consistent with previous observations, there was a negative correlation between the maximum velocity (V(max)) of platelet 5-HT transport and pO2 (r=-0.52, p<0.01). Females had a significantly higher pO2 than males (F=4.9, p<0.05). After accounting for gender differences, addition of pO2 did not add further strength to the regression, given the aforementioned gender differences in pO2. The correlation between unadjusted values for platelet vs brain SERT V(max) was r=0.3, p=0.06.


CONCLUSIONS
These results suggest that a relationship may exist between 5-HT transport in platelets and cortical synaptosomes, when appropriate controls for confounding factors are employed.
Rausch
Jeffrey L
JL
Veterans Administration Hospital, Augusta, Augusta, GA 30912, USA. jeffreyr@mcg.edu
Johnson
Maria E
ME
Li
Junqing
J
Hutcheson
Julian
J
Carr
Benjamin M
BM
Corley
Katina M
KM
Gowans
Amanda B
AB
Smith
Joseph
J
D000328:Adult; D000704:Analysis of Variance; D001692:Biological Transport; D001792:Blood Platelets / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism; D012701:Serotonin / Q000493:pharmacokinetics*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin; D010100:Oxygen
Life Sci. 1982 Aug 9;31(6):583-8
7132568
Toxicol Appl Pharmacol. 1984 Oct;76(1):1-8
6435284
Acta Psychiatr Scand. 1980 Mar;61(S280):97-110
28678363
Biochem Pharmacol. 1973 Feb 1;22(3):311-22
4687129
Crit Care Med. 1996 May;24(5):862-9
8706466
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2542-6
7681602
Prog Neuropsychopharmacol Biol Psychiatry. 1988;12(5):585-94
3222448
Neuropsychopharmacology. 1993 Jun;8(4):315-36
8512620
Life Sci. 1977 Dec 1;21(11):1665-73
600018
Science. 1982 Jun 18;216(4552):1337-9
7079769
J Appl Physiol Respir Environ Exerc Physiol. 1977 Jan;42(1):33-8
833074
Life Sci. 2000 Jun 8;67(3):301-15
10983874
Am J Med Genet. 1999 Feb 5;88(1):83-7
10050973
J Pharmacol Exp Ther. 1987 Apr;241(1):196-203
3572782
Arch Gen Psychiatry. 2000 Aug;57(8):729-38
10920459
Psychiatry Res. 1989 Nov;30(2):125-35
2559421
Psychopharmacology (Berl). 1979 Jan 31;60(2):131-5
154682
Biol Psychiatry. 1982 Jan;17(1):121-3
7059634
Psychopharmacology (Berl). 1982;77(4):332-5
6813892
Brain Res. 1995 Aug 7;688(1-2):121-33
8542298
Brain Res. 1978 May 12;146(2):400-3
647398
Biol Psychiatry. 1997 Jun 15;41(12 ):1156-64
9171906
Neuroscience. 2002;114(3):807-15
12220580
Biol Psychiatry. 2000 Apr 1;47(7):643-9
10745057
Exp Brain Res. 2002 Mar;143(2):191-7
11880895
Br J Psychiatry. 1983 Feb;142:188-92
6839075
Psychiatry Res. 1991 Aug;38(2):97-104
1836639
Brain Res. 1986 Jan 1;362(1):185-8
2417665
Neurosci Lett. 1999 Feb 19;261(3):139-42
10081968
Am J Psychiatry. 1983 Jan;140(1):26-30
6401198
Pharmacol Biochem Behav. 1982 Jul;17(1):159-63
6289359
J Appl Physiol Respir Environ Exerc Physiol. 1981 Jun;50(6):1212-9
7263381
Brain Res Mol Brain Res. 1997 Apr;45(1):13-23
9105666
Arch Gen Psychiatry. 1981 Dec;38(12):1322-6
7316677
J Affect Disord. 1998 Jul;50(1):11-5
9716273
Psychiatry Res. 1998 Jun 30;82(3):161-70
9754440
Brain Res Bull. 1986 Jul;17(1):67-73
3756546
Biol Psychiatry. 1994 Sep 1;36(5):292-9
7993955
Arch Gen Psychiatry. 1993 Oct;50(10):810-8
8215804
Clin Physiol. 2001 Jan;21(1):39-43
11168295
J Clin Psychopharmacol. 2001 Apr;21(2):139-42
11270909
Prog Brain Res. 1986;68:25-31
2882555
Psychopharmacology (Berl). 1979 Oct;65(2):141-7
117482
Psychopharmacology (Berl). 1980 Jan;67(1):1-7
6768073
Arch Gen Psychiatry. 1987 Apr;44(4):320-7
2436589
Nature. 1976 Aug 12;262(5569):596-8
958421
Biol Psychiatry. 1986 Mar;21(3):283-92
3004612
Adv Enzymol Relat Areas Mol Biol. 1967;29:1-32
4881884
J Affect Disord. 1984 Dec;7(3-4):223-33
6151956
Life Sci. 1986 Jun 16;38(24):2193-8
3713444
Biol Psychiatry. 1996 Oct 1;40(7):576-84
8886290
Biol Psychiatry. 1998 Dec 1;44(11):1090-8
9836013
Psychiatry Res. 1988 Jun;24(3):263-9
3406242
Psychiatry Res. 1999 Feb 22;85(2):189-98
10220009
Neuropsychobiology. 2001;44(2):78-83
11490175
Brain Res Mol Brain Res. 1999 Jan 8;63(2):241-7
9878762
Brain Res. 1988 Jul 12;455(2):360-5
2969767
J Pharmacol Exp Ther. 1978 Apr;205(1):118-32
633077
Toxicol Appl Pharmacol. 1983 Sep 15;70(2):188-94
6312629
Am J Psychiatry. 1998 Feb;155(2):207-13
9464199
Life Sci. 1983 Aug 1;33(5):437-42
6877030
Psychiatry Res. 1987 Aug;21(4):355-6
2442787
J Neurochem. 1993 Jun;60(6):2319-22
7684072
J Affect Disord. 1986 May-Jun;10(3):233-9
2943778
Life Sci. 1983 Sep 19;33(12):1149-56
6888169
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Brain Res. 1990 Aug 27;526(1):17-22
2150340
Psychiatry Res. 1986 Oct;19(2):105-12
3786602
Brain Res. 1993 Jun 18;614(1-2):37-44
8348328
Clin Physiol. 1999 Mar;19(2):153-60
10200897
J Neural Transm Gen Sect. 1993;93(1):27-35
8373554
J Am Acad Child Adolesc Psychiatry. 1999 Nov;38(11):1396-402
10560226
Clin Chim Acta. 1978 Jul 1;87(1):165-8
668137
Acta Pharmacol Toxicol (Copenh). 1983 Aug;53(2):166-74
6624484
Environ Health Perspect. 1980 Apr;35:191-8
7408797
Life Sci. 1981 Dec 21;29(25):2649-58
7321781
Biol Psychiatry. 1997 Jan 15;41(2):172-83
9018387
Arch Gen Psychiatry. 1989 Mar;46(3):237-41
2537613
Toxicol Lett. 1984 Aug;22(2):255-9
6474516
J Psychiatry Neurosci. 1995 Jan;20(1):11-9
7865496