title: The addition of fenugreek extract (Trigonella foenum-graecum) to glucose feeding increases muscle glycogen resynthesis after exercise.
abstract: The purpose of this study was to determine the effects of ingesting an oral supplement containing 4-Hydroxyisoleucine (4-OH-Ile, isolated from fenugreek seeds [Trigonella foenum-graecum]) with a glucose beverage on rates of post-exercise muscle glycogen resynthesis in trained male cyclists. Following an overnight fast (12 hr), subjects completed a 90-minute glycogen depletion ride after which a muscle biopsy was obtained from the vastus lateralis. Immediately and 2 hours after the muscle biopsy, subjects ingested either an oral dose of dextrose (Glu) (1.8 g.kg BW(-1)) or 4-OH-Ile supplement (Glu+4-OH-Ile, including 2.0 mg.kg(-1) 4-OH-Ile with the same oral dose of dextrose) with a second muscle biopsy 4 hours after exercise. Post exercise muscle glycogen concentration was similar for both trials. Overall, there was a significant increase in glucose and insulin concentrations from time 0 throughout the majority of the 4-hour recovery period, with no significant differences between the two trials at any time point. Although muscle glycogen concentration significantly increased from immediately post exercise to 4 hr of recovery for both trials, the net rate of muscle glycogen resynthesis was 63% greater during Glu+4-OH-Ile (10.6+/-3.3 vs. 6.5+/-2.6 g.kg wet wt.(-1).hr.(-1) for the Glu+4-OH-Ile and Glu trials, respectively). These data demonstrate that when the fenugreek extract supplement (4-OH-Ile) is added to a high oral dose of dextrose, rates of post-exercise glycogen resynthesis are enhanced above dextrose alone.
authors.affiliation: Department of Health and Human Performance, The University of Montana, Missoula, Montana 59812-1825, USA. brent.ruby@mso.umt.edu
authors.forename: B C
authors.initials: BC
authors.lastname: Ruby
authors.forename: S E
authors.initials: SE
authors.lastname: Gaskill
authors.forename: D
authors.initials: D
authors.lastname: Slivka
authors.forename: S G
authors.initials: SG
authors.lastname: Harger
mesh_terms: D000328:Adult; D001642:Bicycling; D001786:Blood Glucose / Q000032:analysis; D019587:Dietary Supplements; D015444:Exercise; D005947:Glucose / Q000494:pharmacology*; D006003:Glycogen / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000097:blood; D007532:Isoleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D029910:Trigonella / Q000737:chemistry*
chemical_list: C104672:4-hydroxyisoleucine; D001786:Blood Glucose; D007328:Insulin; D010936:Plant Extracts; D007532:Isoleucine; D006003:Glycogen; D005947:Glucose