title: Evaluation of the antioxidant and free radical scavenging properties of Secamone afzelii Rhoem.
abstract: The antioxidant activity of a methanol extract of Secamone afzelii stems was tested using the DPPH assay and the active compound was identified as alpha-tocopherol. HPLC determination showed that 0.12% w/w alpha-tocopherol was present in the plant. The total extract also showed effective free radical scavenging activity in the assay for non-enzymatic lipid peroxidation in liposomes with an IC50 value of 90 microg/mL, with alpha-tocopherol isolated from the plant having an IC50 of 15 microg/mL in the same system, thus demonstrating the presence of other antioxidants.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: A Y
authors.initials: AY
authors.lastname: Mensah
authors.forename: P J
authors.initials: PJ
authors.lastname: Houghton
authors.forename: G N A
authors.initials: GN
authors.lastname: Akyirem
authors.forename: T C
authors.initials: TC
authors.lastname: Fleischer
authors.forename: M L K
authors.initials: ML
authors.lastname: Mensah
authors.forename: K
authors.initials: K
authors.lastname: Sarpong
authors.forename: R
authors.initials: R
authors.lastname: Adosraku
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D029062:Apocynaceae; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000378:metabolism; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018547:Plant Stems
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D008081:Liposomes; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl