Thermostable cellulases from Streptomyces sp.: scale-up production in a 50-l fermenter.
Thermostable cellulase was produced by Streptomyces sp. T3-1 grown in a 50-l fermenter. Maximum cellulase activity was attained on the fourth day when agitation speeds and aeration rates were controlled at 300 rpm and 0.75 vvm, respectively. Maximum enzyme activities were: 148 IU CMCase ml(-1), 45 IU Avicelase ml(-1), and 137 IU beta-glucosidase ml(-1) with productivity of 326 IU l(-1) h(-1), which were 10-32% higher than the values obtained in shake-flask cultures.
Jang
Hung-Der
HD
Department of Food Science, Yuanpei University of Science and Technology, 300, Hsinchu, Taiwan. hungder@mail.yust.edu.tw
Chang
Ku-Shang
KS
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D044602:Cellulases / Q000096:biosynthesis* / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D010865:Pilot Projects; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development*
D044602:Cellulases