title: Anti-inflammatory role of interleukin-15 in Crohn's disease.
abstract: BACKGROUND
  Interleukin (IL)-15 is overexpressed in intestinal tissue with active Crohn's disease (CD). However, its role in the pathogenesis of the disease remains uncertain. We studied the effects of IL-15 on colonic mucosal proinflammatory cytokine response in vitro using organ culture of human colonic explants.
  
  
  METHODS
  Colonic tissue was obtained from (1) resections in pediatric CD patients (inflamed and noninflamed) and (2) rectal biopsies in patients with CD undergoing colonoscopy (n = 31) and controls (n = 9). In preliminary experiments, explants from the resections were cultured in the presence or absence of a simulated T(H)1 stimulation using ionomycin (Io) and phorbol-myristate-acetate (PMA), with or without IL-15, or in medium alone. Rectal biopsies were cultured in the same conditions as above, with or without adding a monoclonal anti-IL-15 neutralizing antibody (mAb). Levels of interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, and IL-2R alpha were measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  IL-15, in the absence of Io + PMA, did not induce the expression of IFN-gamma, TNF-alpha, or IL-2R alpha. Only inflamed explants from resections stimulated with Io + PMA expressed IFN-gamma, TNF-alpha, and IL-2R alpha. This T(H)1 stimulatory effect was inhibited by IL-15 in a dose-dependent fashion. In rectal biopsy explants, inflamed, noninflamed CD, and control tissue responded to stimulation with Io + PMA (P < 0.05) with increased IFN-gamma and TNF-alpha (P < 0.05). This response was again inhibited by IL-15. The inhibitory effect of IL-15 was specifically reversed by anti-IL-15 mAb (P < 0.05). The data for the CD group were also analyzed according to the severity of colonic inflammation and medication use.
  
  
  CONCLUSIONS
  Our results suggest a possible anti-inflammatory role for IL-15 in CD. We postulate that its overexpression in CD potentially represents a protective mechanism against the exaggerated T(H)1 immune response.
authors.affiliation: Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Sainte-Justine Hospital Research Centre, University of Montreal, Montreal, Quebec, Canada.
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Silva
authors.forename: José
authors.initials: J
authors.lastname: Menezes
authors.forename: Colette
authors.initials: C
authors.lastname: Deslandres
authors.forename: Ernest G
authors.initials: EG
authors.lastname: Seidman
mesh_terms: D000293:Adolescent; D001706:Biopsy; D003424:Crohn Disease / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D019409:Interleukin-15 / Q000096:biosynthesis / Q000276:immunology* / Q000494:pharmacology*; D008297:Male; D009924:Organ Culture Techniques
chemical_list: D016207:Cytokines; D019409:Interleukin-15