Generation of a floxed allele of the mouse BMP type II receptor gene.
Bone morphogenetic proteins (BMPs) regulate a wide range of cellular functions that contribute to embryonic development from mesoderm formation to organogenesis. BMP type II receptor (BMPR-II) transduces BMP signals by forming heteromeric complexes with and phosphorylating BMP type I receptors. Heterozygous germline mutations of BMPR-II gene have been identified in patients with familial and sporadic primary pulmonary hypertension, indicating that BMPR-II may contribute to the maintenance of normal pulmonary vascular structure and function. Since embryos homozygous for a null BMPR-II allele died during gastrulation, precluding further studies of BMPR-II function in organ formation and in adult tissues, we generated mice carrying a conditional mutant BMPR-II allele in which exons 4 and 5 were flanked by loxP sequences. We anticipate that studies of mice carrying a floxed BMPR-II allele and a Cre transgene (under the control of a tissue-specific promoter) will enable characterization of the role of BMPR-II in specific cell types during development and in the pathogenesis of cardiovascular diseases.
Beppu
Hideyuki
H
Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.
Lei
Hong
H
Bloch
Kenneth D
KD
Li
En
E
D000483:Alleles; D000818:Animals; D052006:Bone Morphogenetic Protein Receptors, Type II; D002678:Chimera / Q000235:genetics; D018274:Electroporation; D047108:Embryonic Development / Q000235:genetics; D005091:Exons; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D005838:Genotype; D019426:Integrases / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology; D019076:Transgenes
D011956:Receptors, Cell Surface; D017346:Protein Serine-Threonine Kinases; C498219:Bmpr2 protein, mouse; D052006:Bone Morphogenetic Protein Receptors, Type II; C045073:Cre recombinase; D019426:Integrases