GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis.
We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.
Comer
Jason E
JE
Department of Microbiology and Immunology, Medical Research Building, 301 University Blvd., Galveston, TX 77555-1070, USA.
Galindo
Cristi L
CL
Chopra
Ashok K
AK
Peterson
Johnny W
JW
D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology* / Q000633:toxicity; D001408:Bacillus anthracis / Q000378:metabolism*; D001427:Bacterial Toxins / Q000494:pharmacology* / Q000633:toxicity; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D011506:Proteins; C030325:anthrax toxin; D020929:Mitogen-Activated Protein Kinase Kinases
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