title: Chronological changes of neurofilament 200 kDa immunoreactivity in the lateral olfactory tract after transient forebrain ischemia in gerbils.
abstract: This study was carried out to investigate the transient ischemia-induced changes of neurofilament 200 kDa (NF-200) immunoreactivity and protein content in the gerbil lateral olfactory tract (LOT) after 5 min of transient forebrain ischemia. Weak NF-200 immunoreactivity was detectable in the LOT in the sham-operated group. In this group, a few somata of mitral cells showed weak NF-200 immunoreactivity. One day after transient ischemia, NF-200 immunoreactivity in the LOT was increased significantly. NF-200 immunoreactivity in the LOT by 15 days after ischemia was similar to that in the 1 day post-ischemic group. In this time period, strong NF-200 immunoreactivity was expressed in the mitral cell processes, but the immunoreactivity in the mitral cell somata was significantly decreased. Thereafter, NF-200 immunoreactivity in the LOT was decreased significantly by 30 days after ischemic insult. At this time after ischemia, NF-200 immunoreactivity in the mitral cell dendrites was significantly decreased. The result of Western blot study showed that the pattern of NF-200 expression was similar to that of immunohistochemistry after ischemia-reperfusion. Our result suggests that changes of NF-200 protein in the gerbil LOT may be related to response to ischemic damage and that the axonal transport followed transient ischemia may be disturbed.
authors.affiliation: School of Biotechnology and Bionengineering, Kangwon National University, Chunchon 200-701, South Korea.
authors.forename: Hyeon Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Un San
authors.initials: US
authors.lastname: Koh
authors.forename: Jae Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Dae Ho
authors.initials: DH
authors.lastname: Kim
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Baek
authors.initials: B
authors.lastname: Hwang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001369:Axons / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D004195:Disease Models, Animal; D005849:Gerbillinae; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism / Q000503:physiopathology; D009833:Olfactory Pathways / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
chemical_list: D016900:Neurofilament Proteins; C051774:neurofilament protein H