Action and efficacy of p-[131I]iodo-L-phenylalanine on primary human glioma cell cultures and rats with C6-gliomas.
BACKGROUND
Recently we demonstrated high selectivity and long retention of p-[123I]iodo-L-phenylalanine (IPA-123) for diagnostic purposes in gliomas. Here we describe the potency of the iodine-131-labelled analogue p-[131I]iodo-L-phenylalanine (IPA-131) for experimental treatment of gliomas.


METHODS
Two human glioma cell cultures and 15/20 rats with C6-gliomas were exposed to IPA-131.


RESULTS
After 24-h-exposure the cell number of the glioma cell cultures was reduced by 77% and 79%, respectively. Four out of five untreated rats died 8-24 days after implantation and had verifiable tumours. Seven animals died despite therapy, while 8 were soon healthier, gained body weight and were sacrificed 81 days after implantation, at which time survival of the treated animals was significantly prolonged (p < 0.05). At autopsy, instead of tumours there were substance defects, surrounded by pre-existing parenchyma infiltrated by probable tumour cells that were not discernible from reactive astrocytes, lymphocytes, monocytes and multinuclear giant cells.


CONCLUSIONS
IPA-131 has promising activity against human glioma cells in vitro and experimental gliomas in vivo.
Romeike
Bernd F M
BF
Institute for Neuropathology, University of the Saarland, School of Medicine, D-66421 Homburg, Germany. pabrom@uniklinik-saarland.de
Hellwig
Dirk
D
Heimann
Axel
A
Kempski
Oliver
O
Feiden
Wolfgang
W
Kirsch
Carl-Martin
CM
Samnick
Samuel
S
D000818:Animals; D001932:Brain Neoplasms / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000532:radiotherapy; D005910:Glioma / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D007457:Iodine Radioisotopes / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D008297:Male; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D019275:Radiopharmaceuticals / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D010649:Phenylalanine; C049181:4-iodophenylalanine