title: Dual regulatory effects of orexins on sympathetic nerve activity innervating brown adipose tissue in rats.
abstract: This study examined how orexin regulates the activity of the sympathetic nerves that innervate brown adipose tissue (BAT) in rats. Infusion of orexin A at a dose of 0.3 nmol into the third cerebral ventricle decreased BAT sympathetic nerve activity, compared with the effect of PBS (P < 0.05), whereas infusion of orexin B at the same dose caused a significant increase (P < 0.05). Pretreatment with a third cerebral ventricle injection of 2.24 micromol/kg alpha-fluoromethylhistidine, an irreversible inhibitor of the histamine-synthesizing enzyme histidine decarboxylase, attenuated the orexin B-induced response of BAT sympathetic nerve activity, but not that induced by orexin A. These results indicate that orexins may regulate both BAT energy expenditure and thermogenesis through their dual effects on sympathetic nerve activity. In particular, orexin B regulates BAT sympathetic nerve activity via neuronal histamine in the hypothalamus.
authors.affiliation: Department of Internal Medicine I, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama, Oita 879-5593, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Yasuda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Masaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kakuma
authors.forename: Masahide
authors.initials: M
authors.lastname: Hara
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nawata
authors.forename: Isao
authors.initials: I
authors.lastname: Katsuragi
authors.forename: Hironobu
authors.initials: H
authors.lastname: Yoshimatsu
mesh_terms: D002001:Adipose Tissue, Brown / Q000294:innervation*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects* / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007276:Injections, Intraventricular; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D008762:Methylhistidines / Q000494:pharmacology; D009479:Neuropeptides / Q000494:pharmacology*; D000068797:Orexins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013564:Sympathetic Nervous System / Q000187:drug effects*; D013566:Sympathomimetics / Q000494:pharmacology; D020542:Third Ventricle
chemical_list: D004791:Enzyme Inhibitors; D047908:Intracellular Signaling Peptides and Proteins; D008762:Methylhistidines; D009479:Neuropeptides; D000068797:Orexins; D013566:Sympathomimetics; C028309:alpha-fluoromethylhistidine