Purkinje cell loss by OX7-saporin impairs excitatory and inhibitory eyeblink conditioning.
Cerebellar cortical contributions to eyeblink conditioned excitation have been examined extensively. In contrast, very little evidence exists concerning the role of the cerebellar cortex in eyeblink conditioned inhibition. In the current study, rats were given intraventricular infusions of the immunotoxin OX7-saporin to selectively destroy Purkinje cells throughout the cerebellar cortex following excitatory conditioning. After a 2-week postinfusion period, the rats were given reacquisition training. After reacquiring excitatory conditioning, the rats were trained in a feature-negative discrimination procedure to establish conditioned inhibition. Rats treated with OX7-saporin showed impaired reacquisition of excitatory conditioning and acquisition of conditioned inhibition. The results suggest that Purkinje cells play important, but different, roles in conditioned excitation and inhibition in rats.
Nolan
Brian C
BC
Department of Psychology, University of Iowa, Iowa City, IA 52242, USA.
Freeman
John H
JH
D000818:Animals; D000911:Antibodies, Monoclonal; D001767:Blinking / Q000502:physiology*; D002531:Cerebellum / Q000293:injuries* / Q000502:physiology*; D003214:Conditioning, Classical; D018796:Immunoconjugates; D000922:Immunotoxins / Q000633:toxicity; D007266:Inhibition, Psychological; D008297:Male; D009699:N-Glycosyl Hydrolases; D051381:Rats; D020318:Rats, Long-Evans; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins
D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D000922:Immunotoxins; C085346:OX7-saporin; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins
J Neurochem. 1983 Mar;40(3):637-44
6131102
Behav Brain Res. 2000 Apr;109(1):37-47
10699656
J Neurosci. 2000 Jul 15;20(14):5516-25
10884335
Brain Res. 1994 Jul 25;652(1):98-106
7953727
Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1592-7
11805298
Science. 1986 Aug 29;233(4767):941-7
3738519
Brain Res. 1991 May 10;548(1-2):18-22
1868333
J Neurosci. 1993 Apr;13(4):1708-18
8463846
J Comp Physiol Psychol. 1977 Apr;91(2):407-17
404342
Neuroreport. 1994 Jan 31;5(5):649-52
8025262
Behav Brain Res. 1989 Jun 1;33(2):113-64
2765164
J Physiol. 1994 Apr 15;476(2):229-44
8046640
Behav Neurosci. 1995 Oct;109(5):893-902
8554713
Physiol Behav. 1980 Nov;25(5):667-73
7443828
Proc Natl Acad Sci U S A. 1987 Apr;84(7):2101-5
3470780
J Neurosci. 2001 Aug 1;21(15):5715-22
11466443
Neuropharmacology. 1998 Apr-May;37(4-5):471-80
9704988
Brain Res. 1995 Jun 19;683(2):228-41
7552359
J Neurosci. 2002 Oct 15;22(20):8808-18
12388587
Behav Brain Res. 1980 Oct;1(5):397-409
7236350
Behav Brain Res. 1983 Dec;10(2-3):383-92
6661284
J Neurosci. 2005 Jan 26;25(4):889-95
15673669
J Neurosci. 1998 Jul 15;18(14):5498-507
9651230
J Neurocytol. 1985 Jun;14(3):427-52
2864401
J Neurosci. 1996 Apr 15;16(8):2829-38
8786457
J Neurophysiol. 1998 Dec;80(6):3148-62
9862913
Learn Mem. 1997 May-Jun;4(1):130-58
10456059
Neuroscience. 2001;107(3):433-45
11718998
Science. 1984 Jan 20;223(4633):296-9
6701513
J Exp Psychol. 1972 Oct;95(2):408-11
5071916
Integr Physiol Behav Sci. 2002 Oct-Dec;37(4):293-310
12645845
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2731-5
6953427
Brain Res. 1993 Nov 5;627(1):129-40
8293293
J Neurophysiol. 1997 Jan;77(1):86-92
9120599
J Neurosci. 2001 Jun 1;21(11):4081-9
11356896
Learn Mem. 2003 Nov-Dec;10(6):427-55
14657256
Brain Res. 1989 Dec 18;504(2):216-22
2574621
Learn Mem. 1997 May-Jun;4(1):116-29
10456058
Neuron. 2002 Jun 13;34(6):1011-20
12086647
Neurobiol Learn Mem. 1996 Jan;65(1):17-34
8673404
Exp Brain Res. 1987;67(3):569-93
3653317
Brain Res. 1999 Jul 3;833(2):225-33
10375698
J Neurosci. 1993 Apr;13(4):1624-35
8463840
Neurobiol Learn Mem. 1999 May;71(3):325-52
10196110
J Neurosci. 1999 Dec 15;19(24):10940-7
10594075
Exp Brain Res. 1992;88(3):623-38
1587320
Behav Neurosci. 1999 Feb;113(1):204-10
10197920
Neurotoxicology. 2000 Jun;21(3):395-403
10894129
J Neurophysiol. 1994 Oct;72(4):1717-33
7823097
Behav Brain Res. 2003 Jun 16;142(1-2):115-24
12798272
J Neurosci. 1999 Dec 15;19(24):RC45
10594089
Science. 1985 Apr 19;228(4697):346-8
2580350
Behav Neurosci. 2002 Feb;116(1):22-36
11895180
Exp Eye Res. 1992 May;54(5):637-44
1623950
Exp Brain Res. 1986;63(2):341-50
3758251
Behav Brain Res. 1991 Dec 13;46(1):71-81
1786114
Exp Brain Res. 1985;60(1):99-113
4043286
Brain Res. 1993 Apr 9;608(1):67-77
8495350
J Neurosci. 1984 Nov;4(11):2811-22
6502205