title: Ischemia-related changes in galanin expression in the dentate hilar region after transient forebrain ischemia in gerbils.
abstract: Although galanin (GAL) protects hippocampal neurons from ischemic damage, no study has examined ischemia-related changes in endogenous GAL in the hippocampal dentate gyrus. We investigated the chronological changes of GAL, well-known as the potassium channel opener, expression in the dentate gyrus at various times after 5 min of transient forebrain ischemia in gerbils. A few GAL-immunoreactive (IR) neurons were found in the polymorphic layer of the sham-operated group. Three hours after ischemia-reperfusion, the pattern of GAL immunoreactivity was similar to that of the sham-operated group and the number of GAL-IR neurons and immunoreactivity were highest 12 h after ischemic insult. At this time, GAL-IR neurons in the polymorphic layer showed strong GAL immunoreactivity. Thereafter, GAL-IR neurons and immunoreactivity significantly decreased in the dentate hilar region. Four days after ischemic insult, GAL-IR neurons were not detectable. In addition, the results of a Western blot study showed a pattern of GAL expression similar to the immunohistochemical changes. GAL protein content also was highest 12 h after ischemia. In conclusion, the increased expression of endogenous GAL in the dentate gyrus after ischemia is related to response to the ischemic damage.
authors.affiliation: School of Biotechnology and Bioengineering, Kangwon National University, Chunchon, South Korea.
authors.forename: Hyeon Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Dae Ho
authors.initials: DH
authors.lastname: Kim
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Beong Ou
authors.initials: BO
authors.lastname: Lim
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Kyung Hwan
authors.initials: KH
authors.lastname: Bang
authors.forename: Nak Sul
authors.initials: NS
authors.lastname: Seong
authors.forename: Hak Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jong Dai
authors.initials: JD
authors.lastname: Kim
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism*; D019004:Galanin / Q000378:metabolism* / Q000502:physiology; D005849:Gerbillinae; D007150:Immunohistochemistry; D015640:Ion Channel Gating; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D015221:Potassium Channels; D013997:Time Factors
chemical_list: D015221:Potassium Channels; D019004:Galanin