Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes.
The important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in Chlamydomonas reinhardtii suggests that transcriptional profiling of such cells would reveal new flagella-related genes. We have conducted a genome-wide analysis of RNA transcript levels during flagellar regeneration in Chlamydomonas by using maskless photolithography method-produced DNA oligonucleotide microarrays with unique probe sequences for all exons of the 19,803 predicted genes. This analysis represents previously uncharacterized whole-genome transcriptional activity profiling study in this important model organism. Analysis of strongly induced genes reveals a large set of known flagellar components and also identifies a number of important disease-related proteins as being involved with cilia and flagella, including the zebrafish polycystic kidney genes Qilin, Reptin, and Pontin, as well as the testis-expressed tubby-like protein TULP2.
Stolc
Viktor
V
Genome Research Facility, National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035, USA. vstolc@mail.arc.nasa.gov
Samanta
Manoj Pratim
MP
Tongprasit
Waraporn
W
Marshall
Wallace F
WF
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D016680:Genome, Bacterial; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics; D007690:Polycystic Kidney Diseases / Q000235:genetics*; D012038:Regeneration; D014158:Transcription, Genetic; D029961:Zebrafish Proteins / Q000235:genetics
D005136:Eye Proteins; D009687:Nuclear Proteins; C484551:Tulp2 protein, mouse; D029961:Zebrafish Proteins; C471327:ruvbl2 protein, zebrafish
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