Lung cancer in smoking patients inversely alters the activity of hOGG1 and hNTH1.
N-Glycosylases excise the damaged adducts from DNA. 7,8-Dihydro-8-oxoguanine in human cells is repaired by OGG1 and hNTH1. The activities of hOGG1 and hNTH1 were measured, using modified and 32P labelled oligonucleotides, in bronchial biopsy samples of smoking patients with non-small cell lung carcinoma. The activity of hOOG1 was significantly higher in biopsies from tumour tissues compared with intra-individual control samples. On the contrary, the activity of endonuclease III homologue, hNTH1, was lower in tumours compared to controls. These opposing alterations in DNA repair enzymes may affect cancer growth due to the increased formation of AP sites.
Radak
Zsolt
Z
Laboratory of Exercise Physiology, School of Sport Science, Semmelweis University, Budapest, Hungary. radak@mail.hupe.hu
Goto
Sataro
S
Nakamoto
Hideko
H
Udud
Katalin
K
Papai
Zsolt
Z
Horvath
Ildiko
I
D000368:Aged; D001706:Biopsy; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045647:DNA Glycosylases / Q000378:metabolism*; D004260:DNA Repair; D043224:Deoxyribonuclease (Pyrimidine Dimer) / Q000378:metabolism*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000009:adverse effects*
D043224:Deoxyribonuclease (Pyrimidine Dimer); C468347:NTHL1 protein, human; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human