Function and expression of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli.
The Escherichia coli yjhA (renamed nanC) gene encodes a protein of the KdgM family of outer membrane-specific channels. It is transcribed divergently from fimB, a gene involved in the site-specific inversion of the region controlling transcription of the fimbrial structural genes but is separated from it by one of the largest intergenic regions in E. coli. We show that nanC expression is induced by N-acetylneuraminic acid and modulated by N-acetylglucosamine. This regulation occurs via the NanR and NagC regulators, which also control fimB expression. nanC expression is also activated by the regulators cyclic AMP-catabolite activator protein, OmpR, and CpxR. When the NanC protein was reconstituted into liposomes, it formed channels with a conductance of 450 pS at positive potential and 300 to 400 pS at negative potential in 800 mM KCl. The channels had a weak anionic selectivity. In an ompR background, where the general porins OmpF and OmpC are absent, NanC is required for growth of E. coli on N-acetylneuraminic acid as the sole carbon source. All these results suggest that NanC is an N-acetylneuraminic acid outer membrane channel protein.
Condemine
Guy
G
Unité Microbiologie et Génétique, UMR-CNRS-INSA-UCB 5122, Villeurbanne, France. guy.condemine@insa-lyon.fr
Berrier
Catherine
C
Plumbridge
Jacqueline
J
Ghazi
Alexandre
A
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D018272:Porins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000502:physiology
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D018272:Porins; D019158:N-Acetylneuraminic Acid
Microbiol Mol Biol Rev. 2004 Mar;68(1):132-53
15007099
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656
14665678
Science. 2004 Jun 4;304(5676):1506-9
15178802
J Bacteriol. 1983 Oct;156(1):62-9
6311806
J Biol Chem. 1987 Jan 25;262(3):1412-9
3027089
J Biol Chem. 1988 Feb 15;263(5):2493-9
3276691
FEBS Lett. 1989 Dec 18;259(1):27-32
2480919
Mol Microbiol. 1991 Aug;5(8):2053-62
1766379
Nature. 1992 Aug 27;358(6389):727-33
1380671
Science. 1995 Jan 27;267(5197):512-4
7824948
FEBS Lett. 1996 Feb 26;381(1-2):111-4
8641415
J Bacteriol. 1997 Oct;179(20):6228-37
9335267
Mol Microbiol. 1997 Dec;26(5):1141-3
9426150
J Bacteriol. 1999 Jan;181(1):47-54
9864311
Microbiology. 2004 Nov;150(Pt 11):3571-90
15528647
Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16322-7
15534208
J Biol Chem. 2000 Oct 20;275(42):33068-76
10913115
EMBO J. 2000 Nov 15;19(22):5980-8
11080145
EMBO J. 2001 Apr 2;20(7):1508-18
11285215
J Bacteriol. 2001 Dec;183(24):7213-23
11717281
Infect Immun. 2002 Mar;70(3):1391-402
11854225
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2287-92
11830644
J Biol Chem. 2002 Mar 8;277(10):7936-44
11773048
Mol Microbiol. 2002 Oct;46(1):281-91
12366850
Biochem J. 2003 Jun 1;372(Pt 2):329-34
12603200
Mol Microbiol. 2003 Aug;49(4):1109-18
12890032
J Bacteriol. 2003 Aug;185(16):4806-15
12897000
Proc Natl Acad Sci U S A. 2004 May 11;101(19):7427-32
15123798