title: Functional variability of Rev response element in HIV-1 primary isolates.
abstract: We have previously studied sequence heterogeneity of HIV-1 Rev response element (RRE), and showed uneven variations in different stem-loops of both primary sequence and secondary structure. Here we studied the functional variation of RRE clones from a set of 10 primary isolates, and demonstrated a variation in the function of these RRE clones on the expression of Gag proteins from a truncated HIV-1 genome. The difference in Gag level was, in part, if not exclusively, resulted from the differential efficiency of RNA transport and enhancing of translation. These data suggested that variation of HIV-1 RRE may play a role in regulation of viral replication rate in HIV-1 primary isolates.
authors.affiliation: Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Angsana
authors.initials: A
authors.lastname: Phuphuakrat
authors.forename: Prasert
authors.initials: P
authors.lastname: Auewarakul
mesh_terms: D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000096:biosynthesis; D015752:Genes, env / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D034443:RNA Transport; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014779:Virus Replication
chemical_list: D015683:Gene Products, gag; D012367:RNA, Viral
references.citation: AIDS Res Hum Retroviruses. 2003 Jul;19(7):569-74
references.pmid: 12908934
references.citation: Genes Dev. 1991 May;5(5):808-19
references.pmid: 1827422
references.citation: J Biomed Sci. 1996 Apr;3(2):82-91
references.pmid: 11725088
references.citation: Annu Rev Microbiol. 1998;52:491-532
references.pmid: 9891806
references.citation: J Virol. 1993 May;67(5):2871-8
references.pmid: 8474177
references.citation: Science. 1989 Dec 22;246(4937):1625-9
references.pmid: 2688093
references.citation: Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):683-7
references.pmid: 1992459
references.citation: J Mol Biol. 1996 May 24;258(5):813-26
references.pmid: 8637012
references.citation: Cell. 1990 Feb 23;60(4):675-83
references.pmid: 2406030
references.citation: Oncogene. 1997 Oct;15(18):2145-50
references.pmid: 9393972
references.citation: J Virol. 1992 Jan;66(1):150-9
references.pmid: 1727477
references.citation: Nucleic Acids Res. 1999 Nov 1;27(21):4208-17
references.pmid: 10518612
references.citation: Nat Med. 1999 Jun;5(6):635-42
references.pmid: 10371501
references.citation: J Mol Biol. 1994 Aug 12;241(2):193-207
references.pmid: 8057359
references.citation: J Virol. 1989 Nov;63(11):4875-81
references.pmid: 2677405
references.citation: Adv Pharmacol. 2000;48:251-98
references.pmid: 10987094
references.citation: Nature. 1989 Mar 16;338(6212):254-7
references.pmid: 2784194
references.citation: Mol Cell Biol. 1992 Mar;12(3):1375-86
references.pmid: 1545819
references.citation: Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):973-8
references.pmid: 9023367
references.citation: Proc Natl Acad Sci U S A. 1989 Mar;86(5):1495-9
references.pmid: 2784208
references.citation: AIDS Res Hum Retroviruses. 1996 Oct 10;12(15):1443-56
references.pmid: 8893052
references.citation: J Virol. 1990 Dec;64(12):5966-75
references.pmid: 2243382
references.citation: Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):709-14
references.pmid: 9892698
references.citation: Genes Dev. 1990 Aug;4(8):1357-64
references.pmid: 2227413