The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation.
It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547-1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous "precentrioles" become morphologically recognizable centrioles before mitosis. De novo-assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo-formed centrioles do not mature if they are assembled in S phase-arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway.
La Terra
Sabrina
S
Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA.
English
Christopher N
CN
Hergert
Polla
P
McEwen
Bruce F
BF
Sluder
Greenfield
G
Khodjakov
Alexey
A
D002453:Cell Cycle / Q000502:physiology*; D002502:Centrioles / Q000502:physiology*; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D016196:S Phase / Q000502:physiology
Biochim Biophys Acta. 1996 Oct 9;1288(2):F55-78
8876633
J Cell Biol. 1982 Jun;93(3):938-49
7119006
Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9141-6
9256449
Cell Motil Cytoskeleton. 1997;38(4):311-7
9415373
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9295-300
9689074
Cancer Res. 1998 Sep 1;58(17):3974-85
9731511
J Cell Biol. 1998 Dec 14;143(6):1575-89
9852152
Cell Motil Cytoskeleton. 1998;41(4):281-8
9858153
Methods Cell Biol. 1999;61:81-111
9891310
Methods Cell Biol. 1999;61:297-315
9891321
Biol Cell. 1999 Jul;91(6):451-60
10519005
Curr Biol. 2000 Jan 27;10(2):59-67
10662665
J Cell Biol. 2000 Apr 17;149(2):317-30
10769025
Trends Cell Biol. 2001 Jan;11(1):18-21
11146294
Science. 2001 Feb 23;291(5508):1547-50
11222860
Science. 2001 Feb 23;291(5508):1550-3
11222861
Curr Biol. 2001 Mar 6;11(5):308-17
11267867
J Cell Biol. 2001 Apr 2;153(1):237-42
11285289
Trends Cell Biol. 2001 Oct;11(10):413-9
11567874
Cell Motil Cytoskeleton. 2002 Jun;52(2):66-81
12112149
Oncogene. 2002 Sep 9;21(40):6234-40
12214254
J Cell Biol. 2002 Sep 30;158(7):1171-81
12356862
Nat Rev Cancer. 2002 Nov;2(11):815-25
12415252
J Cell Biol. 2002 Dec 9;159(5):731-7
12473683
Nat Cell Biol. 2003 Jun;5(6):539-44
12766773
Dev Biol. 2003 Aug 15;260(2):298-313
12921733
J Cell Biol. 2003 Sep 29;162(7):1211-21
14504268
Eur J Cell Biol. 1982 Aug;28(1):68-76
7128618
Cell Motil Cytoskeleton. 1986;6(4):355-62
2875802
Int Rev Cytol. 1987;100:49-92
3549609
Science. 1989 Nov 3;246(4930):629-34
2683079
Cell. 1991 Nov 1;67(3):495-504
1934057
Biol Cell. 1991;72(1-2):61-6
1756312
Science. 1992 Apr 10;256(5054):219-21
1566068
Nature. 1995 Dec 7;378(6557):578-83
8524390
Nature. 1995 Dec 7;378(6557):638-40
8524401
J Cell Sci. 1972 Sep;11(2):521-41
5076360
J Cell Sci. 1996 Dec;109 ( Pt 13):3089-102
9004043