title: Oxidative state in platelets and erythrocytes in aging and Alzheimer's disease.
abstract: Several studies have shown involvement of peroxynitrite anion, a potent oxidative agent, in Alzheimer's disease (AD) neuropathology. Herein, we assessed in platelets and erythrocytes of AD patients, age-matched and young adults controls: thiobarbituric acid-reactive substances (TBARS) production; superoxide dismutase (SOD), nitric oxide synthase (NOS) and Na,K-ATPase activities; cyclic GMP (cGMP) content, both basal and after sodium nitroprusside (SNP) stimulation. Aging was associated with an increase in TBARS production and NOS activity, a decrease in basal cGMP content and no change in SOD and Na,K-ATPase activities. AD patients, compared to aged controls, have: increase in TBARS production and in NOS, SOD and Na,K-ATPase activities but no alteration in basal cGMP content. SNP increased cGMP platelets production in all groups. In conclusion, we demonstrated in platelets and erythrocytes a disruption in systemic modulation of oxidative stress in aging and with more intensity in AD.
authors.affiliation: Department of Pharmacology, School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Elisa Mitiko
authors.initials: EM
authors.lastname: Kawamoto
authors.forename: Carolina Demarchi
authors.initials: CD
authors.lastname: Munhoz
authors.forename: Isaias
authors.initials: I
authors.lastname: Glezer
authors.forename: Valéria Santoro
authors.initials: VS
authors.lastname: Bahia
authors.forename: Paulo
authors.initials: P
authors.lastname: Caramelli
authors.forename: Ricardo
authors.initials: R
authors.lastname: Nitrini
authors.forename: Renata
authors.initials: R
authors.lastname: Gorjão
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
authors.forename: Cristoforo
authors.initials: C
authors.lastname: Scavone
authors.forename: Tania
authors.initials: T
authors.lastname: Marcourakis
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000544:Alzheimer Disease / Q000097:blood* / Q000473:pathology; D001792:Blood Platelets / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010100:Oxygen / Q000097:blood*; D017382:Reactive Oxygen Species / Q000097:blood*
chemical_list: D017382:Reactive Oxygen Species; D010100:Oxygen