[X chromosome inactivation pattern in elderly women over 70 years of age].
In the present study we have analyzed X chromosome inactivation patterns in 40 women aged from 74 to 85 years (mean age 78 years). The control group was 36 women (mean age 30 years). The most common AR-assay was used to determine X-inactivation patterns (the study of methylation patterns of HpaII site in human androgen receptor gene (HUMARA) by quantative PCR). The age dependence of X-inactivation was not observed. We have detected skewed X-inactivation in three women among 40 (7.5%) elderly women comparing to two women among 36 (5.5%) women from control group. The difference was not found to be statistically significant. We made a suggestion that higher incidence of skewed X-inactivation in elderly women revealed by previous studies could occur due to some experimental ambiguities as heterogeneity of the group studied; inclusion of women having relatives with genetic abnormalities associated with skewed X-inactivation patterns; the difference of X chromosome inactivation skewing determination. We conclude that present study does not show X chromosome inactivation to be age dependent.
Iurov
I Iu
IIu
Willard
L
L
Vorsanova
S G
SG
Demidova
I A
IA
Goĭko
E A
EA
Shal'nova
S A
SA
Shkol'nikova
M A
MA
Olfer'ev
A M
AM
Iurov
Iu B
IuB
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics*; D002648:Child; D041321:Chromosomes, Human, X / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004303:Dosage Compensation, Genetic; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D004247:DNA