[HPLC determination of diltiazem in human serum and its pharmacokinetic parameters].
A simple and sensitive reversed-phase liquid chromatographic method has been developed and validated for the analysis of diltiazem in human serum and the study of pharmacokinetics of the drug in human body. Diltiazem and diazepam (internal standard) in serum were extracted with hexane-chloroform-isopropanol (60:40:5, V/V), and then measured by HPLC using a Spherisorb C18 column as stationary phase and a methanol-water triethylamine as mobile phase. Diltiazem was quantified by ultraviolet absorbance at 239 nm. The method proved to be linear in the clinical range of 15-300 microg/L with a regression coefficient of 0.9997. The lower limit of detection of diltiazem in serum was 3 microg/L. Intra-day and inter-day coefficients of variation of assay for diltiazem in serum were 3.5%-6.8% (n=7) and 6.2%-8.4% (n=5), respectively. The recoveries of diltiazem were 91%-104% for serum. The method has been used to determine diltiazem in serum samples from eight volunteers and provided data on the pharmacokinetics of the drug. The results inferred that diltiazem is absorbed rapidly and had a relatively short half-life time in healthy individuals. The data obtained was fitted with PKBP-N1 program on computer to study the pharmacokinetics. The results showed that the peak level in serum averaged 118.5 microg/L +/- 14.3 microg/L at 3.1 h +/- 0.4 h, and the areas under the drug concentration curves (AUC) was 793.1 microg x h/L +/- 83.1 microg x h/L.
Li
K
K
Department of Instrumental Analysis, General Hospital of Nanjing Command, PLA, Nanjing, 210002.
Zhang
X
X
Yuan
Y
Y
Luo
N
N
Tan
L
L
D000959:Antihypertensive Agents / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004110:Diltiazem / Q000097:blood* / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D055815:Young Adult
D000959:Antihypertensive Agents; D004110:Diltiazem