Treatment with dehydroepiandrosterone prevents oxidative stress induced by 3-nitropropionic acid in synaptosomes.
This study evaluates the antioxidative effect of dehydroepiandrosterone (DHEA) treatment on 3-nitropropionic acid (3-NPA)-induced oxidative stress in striatal and brain cortex synaptosomes. The oxidative derangement was confirmed by a high level of lipid peroxidation products and protein carbonyls, as well as by an enhanced superoxide dismutase activity (p < 0.001). These changes were partially prevented by DHEA. Moreover, 3-NPA induced a drop in succinate dehydrogenase activity, while DHEA treatment restored the succinate dehydrogenase activity. These results show that DHEA reduces oxidative stress in synaptosomes isolated from the brain of 3-NPA-treated rats, and they suggest this neurosteroid may protect mitochondrial and maintain synaptic integrity against damage induced by this acid.
Túnez
Isaac
I
Department of Biochemistry and Molecular Biology, School of Medicine, University of Cordoba, Cordoba, Spain.
Muñoz
M Carmen
MC
Montilla
Pedro
P
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009574:Nitro Compounds; D018384:Oxidative Stress / Q000187:drug effects*; D011422:Propionates; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000096:biosynthesis; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D009574:Nitro Compounds; D011422:Propionates; D003687:Dehydroepiandrosterone; D013482:Superoxide Dismutase; D013385:Succinate Dehydrogenase; C015392:3-nitropropionic acid