title: HIV-1-derived self-inactivating lentivirus vector induces megakaryocyte lineage-specific gene expression.
abstract: Pluripotent, self-renewing, hematopoietic stem cells are considered good targets for gene modification to treat a wide variety of disorders. However, as many genes are expressed in a stage-specific manner during the course of hematopoietic development, it is necessary to establish a lineage-specific gene expression system to ensure the proper expression of transduced genes in hematopoietic stem cells. In this study, we constructed a VSV-G-pseudotyped, human immunodeficiency virus type 1-based, self-inactivating lentivirus vector that expressed green fluorescent protein (GFP) under the control of the human CD41 (glycoprotein 2b; GP2b) promoter; this activity is restricted to megakaryocytic lineage cells. The recombinant virus was used to infect human peripheral blood CD34+ (hematopoietic stem/progenitor) cells, and lineage-specific gene expression was monitored with GFP measurements. The analysis by FACS determined that GFP expression driven by the GP2b promoter was restricted to megakaryocytic progenitors and was not present in erythrocytes. Furthermore, in the hematopoietic colony-forming assay, GFP expression was restricted to colony-forming units-megakaryocyte (CFU-Meg) colonies under the control of the GP2b promoter, whereas all myeloid colonies (burst-forming units-erythroid, colony-forming units-granulocyte-macrophage, and CFU-Meg) expressed GFP when the transgene was regulated by the cytomegalovirus promoter. These results demonstrated lineage-specific expression after gene transduction of hematopoietic stem cells. The application of this vector system should provide a useful tool for gene therapy to treat disorders associated with megakaryocyte (platelet) dysfunction.
authors.affiliation: Department of Microbiology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8506, Japan.
authors.forename: Kazuta
authors.initials: K
authors.lastname: Yasui
authors.forename: Rika A
authors.initials: RA
authors.lastname: Furuta
authors.forename: Kayoko
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Tani
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Fujisawa
mesh_terms: D001854:Bone Marrow Cells / Q000276:immunology; D002454:Cell Differentiation; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D039421:Integrin alpha2; D016086:Lentivirus / Q000235:genetics / Q000502:physiology*; D008533:Megakaryocytes / Q000378:metabolism / Q000821:virology*; D008562:Membrane Glycoproteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014162:Transfection; D019076:Transgenes
chemical_list: C056564:ITGA2B protein, human; D039421:Integrin alpha2; D008562:Membrane Glycoproteins; D049452:Green Fluorescent Proteins