Impact of taurolidin and octreotide on liver metastasis and lipid peroxidation after laparoscopy in chemical induced ductal pancreatic cancer.
BACKGROUND
There is controversial discussion whether metastasis initiated by laparoscopy with carbon dioxide might be prevented by instillation of taurolidin or radical scavengers like the somatostatin analogue Octreotide. Therefore we evaluated the effects of laparoscopic lavage with taurolidin and Octreotide on liver metastasis after staging laparoscopy in ductal pancreatic cancer.


METHODS
In 60 Syrian hamsters pancreatic adenocarcinoma was induced by weekly subcutanous injection of 10 mg N-nitrosobis-2-oxopropylamin/kg body weight for 10 weeks. In the 16th week laparoscopic staging biopsy by use of carbon dioxide was performed. Finally animals underwent abdominal irrigation with saline (gr.1, n = 20), taurolidin (0.5%) (gr.2, n = 20) or Octreotide (gr.3, n = 20). In week 25 animals were sacrificed, pancreas and liver were analysed.


RESULTS
Size of pancreatic carcinomas was decreased in the taurolidin gr. compared to the other two groups. Furthermore the number of liver metastasis per animal was reduced after lavage with taurolidin (2 +/- 2) and Octreotide (2.5 +/- 2) compared to saline irrigation (4 +/- 4) (p < 0.05). Additionally the incidence of port site metastases was significantly reduced in the taurolidin group. Activity of antioxidative enzyme superoxide dismutase (SOD) was increased while concentration of products of lipidperoxidation was decreased in non-metastatic liver after taurolidin irrigation compared to saline or Octreotide irrigation.


CONCLUSIONS
Taurolidin irrigation during laparoscopy might be a new concept to reduce the number of liver metastasis and port site metastases in pancreatic cancer.
Kilian
Maik
M
Clinic of General, Visceral, Vascular and Thoracic Surgery, Humboldt-University, Schumannstr. 2/21, 10117 Berlin, Germany.
Gregor
Jan Ilga
JI
Heukamp
Ina
I
Braumann
Chris
C
Guski
Hans
H
Schimke
Ingolf
I
Walz
Martin Karl
MK
Jacobi
Christoph Andreas
CA
Wenger
Frank Axel
FA
D000230:Adenocarcinoma / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D021441:Carcinoma, Pancreatic Ductal / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D006224:Cricetinae; D010535:Laparoscopy / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008113:Liver Neoplasms / Q000473:pathology / Q000517:prevention & control* / Q000556:secondary; D008297:Male; D008647:Mesocricetus; D009362:Neoplasm Metastasis / Q000517:prevention & control; D015282:Octreotide / Q000627:therapeutic use*; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D013654:Taurine / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D013829:Thiadiazines / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D013829:Thiadiazines; D013654:Taurine; C012566:taurolidine; D015282:Octreotide
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