title: A second field metachronous Merkel cell carcinoma of the lip and the palatine tonsil confirmed by microarray-based comparative genomic hybridisation.
abstract: Merkel cell carcinoma was diagnosed in a 79-year-old Caucasian woman. The tumour was localised to the upper lip and was in stage T2. After successful cryosurgery and a 7-year tumour-free period, a new tumour developed in her palatine tonsil. Histologically and immunohistochemically, this resembled the tumour in the lip. The regional lymph nodes were devoid of metastasis. The paraffin-embedded material of the two tumours and the unaffected lymphatic tissue were analysed with DNA microarrays for comparative genomic hybridisation to assess the genetic relationship of the tumours. In both tumours, regions on 2p and 10p were commonly over-represented, while 41 regions on chromosomes 1-4, 6, 8-9, 11 and 14-22 were commonly under-represented. Chromosomes 1, 3, 4, 16-18 and X were most frequently involved in the DNA losses. In gene copy numbers in the two tumours, 31 chromosome locations were found to be differently affected. The partly similar and partly different molecular patterns indicated a genetic relationship between the tumours and excluded the possibility that the tonsillar tumour was a metastasis. The findings suggest that a genetically altered field was the reason for the development of the tonsillar cancer; thus, it can be regarded pathogenetically as a second field tumour.
authors.affiliation: Department of Oral Medicine, University of Szeged Faculty of Medicine, Szeged, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Nagy
authors.forename: Liliána Z
authors.initials: LZ
authors.lastname: Fehér
authors.forename: István
authors.initials: I
authors.lastname: Sonkodi
authors.forename: József
authors.initials: J
authors.lastname: Lesznyák
authors.forename: Béla
authors.initials: B
authors.lastname: Iványi
authors.forename: László G
authors.initials: LG
authors.lastname: Puskás
mesh_terms: D000368:Aged; D015266:Carcinoma, Merkel Cell / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D002869:Chromosome Aberrations; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009378:Neoplasms, Multiple Primary / Q000235:genetics / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D014067:Tonsillar Neoplasms / Q000235:genetics / Q000473:pathology*
references.citation: Int J Cancer. 2002 May 20;99(3):352-60
references.pmid: 11992403
references.citation: Cancer Res. 2003 Apr 15;63(8):1727-30
references.pmid: 12702551
references.citation: Int J Cancer. 2002 Sep 10;101(2):137-45
references.pmid: 12209990
references.citation: Genomics. 1992 Jul;13(3):718-25
references.pmid: 1639399
references.citation: J Oral Maxillofac Surg. 1998 Aug;56(8):984-8
references.pmid: 9710195
references.citation: Mod Pathol. 1990 Sep;3(5):631-4
references.pmid: 2172961
references.citation: J Laryngol Otol. 1992 Dec;106(12):1100-2
references.pmid: 1487674
references.citation: Anat Rec A Discov Mol Cell Evol Biol. 2003 Mar;271(1):225-39
references.pmid: 12552639
references.citation: J Oral Pathol Med. 1999 Feb;28(2):88-91
references.pmid: 9950256
references.citation: Nat Genet. 2001 Dec;29(4):459-64
references.pmid: 11694878
references.citation: Oral Surg Oral Med Oral Pathol. 1992 Aug;74(2):193-200
references.pmid: 1508528
references.citation: Laryngorhinootologie. 2001 Nov;80(11):687-9
references.pmid: 11702286
references.citation: Genes Chromosomes Cancer. 2000 Aug;28(4):395-403
references.pmid: 10862048
references.citation: Cancer. 1972 Sep;30(3):737-41
references.pmid: 4342409
references.citation: Anal Biochem. 2002 Jun 15;305(2):279-81
references.pmid: 12054458
references.citation: J Cutan Pathol. 1996 Oct;23(5):391-7
references.pmid: 8915847
references.citation: Int J Oral Maxillofac Surg. 1998 Jun;27(3):213-4
references.pmid: 9662016
references.citation: Nat Genet. 2001 Nov;29(3):263-4
references.pmid: 11687795
references.citation: Nucleic Acids Res. 2002 Feb 15;30(4):e15
references.pmid: 11842121
references.citation: Head Neck. 2002 Feb;24(2):198-206
references.pmid: 11891950
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Mod Pathol. 2004 May;17(5):561-7
references.pmid: 15001998
references.citation: J Invest Dermatol. 1972 Jun;58(6):381-7
references.pmid: 5030658
references.citation: Eur Arch Otorhinolaryngol. 2003 May;260(5):258-60
references.pmid: 12750915
references.citation: J Oral Pathol Med. 1996 Jan;25(1):44-7
references.pmid: 8850357
references.citation: Br J Cancer. 2004 Feb 23;90(4):860-5
references.pmid: 14970865
references.citation: Cancer Res. 1996 Jun 1;56(11):2488-92
references.pmid: 8653682
references.citation: Cancer. 1953 Sep;6(5):963-8
references.pmid: 13094644
references.citation: Int J Cancer. 2001 May 1;92 (3):409-13
references.pmid: 11291079
references.citation: Oral Surg Oral Med Oral Pathol. 1988 Jan;65(1):71-5
references.pmid: 3422398
references.citation: Crit Rev Oral Biol Med. 2003;14(5):363-9
references.pmid: 14530304
references.citation: Semin Cutan Med Surg. 2004 Mar;23(1):46-53
references.pmid: 15095915
references.citation: Arch Otolaryngol Head Neck Surg. 1999 Aug;125(8):907-11
references.pmid: 10448739
references.citation: Biotechniques. 2002 Oct;33(4):898-900, 902, 904
references.pmid: 12398199
references.citation: FEBS Lett. 2003 Feb 11;536(1-3):35-40
references.pmid: 12586334