Culture system for embryos of blue-breasted quail from the blastoderm stage to hatching.
The blue-breasted quail (Coturnix chinensis), the smallest species in the order Galliforms, is a candidate model animal for avian developmental engineering because it is precocious and prolific. This species requires 17 days to hatch and 8 to 9 weeks to mature to an adult body weight of about 50 g, whereas the Japanese quail (Coturnix japonica) requires 16 days to hatch and 6 to 8 weeks to mature to an adult body weight of 100 to 150 g. The early embryo is the most challenging embryonic stage in terms of culture and manipulation for avian biotechnology. We have evaluated various conditions for the culture of blue-breasted quail embryos from the blastoderm stage to hatching. A hatchability rate of 26% (10/39) is among the best of the various culture conditions examined in the present study and the embryo culture system should facilitate advances in avian biotechnology.
Ono
Tamao
T
Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan.
Nakane
Yoshifumi
Y
Wadayama
Takahiro
T
Tsudzuki
Masaoki
M
Arisawa
Kenjiro
K
Ninomiya
Shoko
S
Suzuki
Toshihiko
T
Mizutani
Makoto
M
Kagami
Hiroshi
H
D000818:Animals; D001709:Biotechnology; D001756:Blastoderm; D003370:Coturnix; D046149:Embryo Culture Techniques / Q000379:methods*; D023421:Models, Animal; D011784:Quail / Q000196:embryology*