Enhanced nisin production by increasing genes involved in nisin Z biosynthesis in Lactococcus lactis subsp. lactis A164.
Nisin Z production in Lactococcus lactis subsp. lactis A164 was improved by introducing multicopy genes, nisZ, nisRK, or nisFEG, involved in nisin biosynthesis into A164 strain. A similar growth profile was obtained from all strains tested. However, the cells expressing nisRK produced 25,000 AU nisin Z ml(-1) compared to 16,000 AU ml(-1) by the control strain. Northern blot analysis revealed that over-expression of nisRK promoted the transcription of the nisZ gene. The A164 strain expressing multicopy nisFEG also had an increased nisin Z production (25,000 AU ml (-1)) but produced the nisin more slowly than the cells expressing multicopy nisRK.
Cheigh
Chan-Ick
CI
Department of Biotechnology, Yonsei University, Seoul 120-749, South Korea.
Park
Hoon
H
Choi
Hak-Jong
HJ
Pyun
Yu-Ryang
YR
D015152:Blotting, Northern; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D009561:Nisin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D013696:Temperature
D009841:Oligonucleotides; D012329:RNA, Bacterial; D011994:Recombinant Proteins; D009561:Nisin; C071364:nisin Z