title: On the role of Cro in lambda prophage induction.
abstract: The lysogenic state of bacteriophage lambda is exceptionally stable yet the prophage is readily induced in response to DNA damage. This delicate epigenetic switch is believed to be regulated by two proteins; the lysogenic maintenance promoting protein CI and the early lytic protein Cro. First, we confirm, in the native configuration, the previous observation that the DNA loop mediated by oligomerization of CI bound to two distinct operator regions (O(L) and O(R)), increases repression of the early lytic promoters and is important for stable maintenance of lysogeny. Second, we show that the presence of the cro gene might be unimportant for the lysogenic to lytic switch during induction of the lambda prophage. We revisit the idea that Cro's primary role in induction is instead to mediate weak repression of the early lytic promoters.
authors.affiliation: National Cancer Institute, Bethesda, MD 20892-4255, USA.
authors.forename: Sine L
authors.initials: SL
authors.lastname: Svenningsen
authors.forename: Nina
authors.initials: N
authors.lastname: Costantino
authors.forename: Donald L
authors.initials: DL
authors.lastname: Court
authors.forename: Sankar
authors.initials: S
authors.lastname: Adhya
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics / Q000502:physiology*; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D044127:Epigenesis, Genetic; D048168:Escherichia coli K12 / Q000821:virology; D017930:Genes, Reporter; D005814:Genes, Viral; D008242:Lysogeny / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D054334:Viral Regulatory and Accessory Proteins; D014775:Virus Activation / Q000235:genetics / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins
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