Glucose-dependent insulinotropic polypeptide is expressed in adult hippocampus and induces progenitor cell proliferation.
The hippocampal dentate gyrus (DG) is an area of active proliferation and neurogenesis within the adult brain. The molecular events controlling adult cell genesis in the hippocampus essentially remain unknown. It has been reported previously that adult male and female rats from the strains Sprague Dawley (SD) and spontaneously hypertensive (SHR) have a marked difference in proliferation rates of cells in the hippocampal DG. To exploit this natural variability and identify potential regulators of cell genesis in the hippocampus, hippocampal gene expression from male SHR as well as male and female SD rats was analyzed using a cDNA array strategy. Hippocampal expression of the gene-encoding glucose-dependent insulinotropic polypeptide (GIP) varied strongly in parallel with cell-proliferation rates in the adult rat DG. Moreover, robust GIP immunoreactivity could be detected in the DG. The GIP receptor is expressed by cultured adult hippocampal progenitors and throughout the granule cell layer of the DG, including progenitor cells. Thus, these cells have the ability to respond to GIP. Indeed, exogenously delivered GIP induced proliferation of adult-derived hippocampal progenitors in vivo as well as in vitro, and adult GIP receptor knock-out mice exhibit a significantly lower number of newborn cells in the hippocampal DG compared with wild-type mice. This investigation demonstrates the presence of GIP in the brain for the first time and provides evidence for a regulatory function for GIP in progenitor cell proliferation.
Nyberg
Jenny
J
The Arvid Carlsson Institute for Neuroscience at the Institute of Clinical Neuroscience, Göteborg University, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden. jenny.nyberg@neuro.gu.se
Anderson
Michelle F
MF
Meister
Björn
B
Alborn
Ann-Marie
AM
Ström
Anna-Karin
AK
Brederlau
Anke
A
Illerskog
Ann-Christin
AC
Nilsson
Ola
O
Kieffer
Timothy J
TJ
Hietala
Max Albert
MA
Ricksten
Anne
A
Eriksson
Peter S
PS
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005749:Gastric Inhibitory Polypeptide / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D020869:Gene Expression Profiling; D006973:Hypertension / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D011918:Rats, Inbred SHR; D017207:Rats, Sprague-Dawley; D011964:Receptors, Gastrointestinal Hormone / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D013234:Stem Cells / Q000166:cytology*
D011964:Receptors, Gastrointestinal Hormone; D005749:Gastric Inhibitory Polypeptide; C041086:gastric inhibitory polypeptide receptor
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9415-20
10430957
Cell Tissue Res. 1999 Nov;298(2):287-93
10571117
Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14843-7
10611300
Life Sci. 2000;66(2):91-103
10666005
Endocrinology. 2000 Mar;141(3):1228-35
10698200
J Neurosci. 2000 Apr 15;20(8):2896-903
10751442
Endocrinology. 2000 Oct;141(10):3710-6
11014226
Neurosci Lett. 2000 Oct 27;293(2):91-4
11027841
Endocr Rev. 2000 Dec;21(6):619-70
11133067
Nat Neurosci. 2001 Feb;4(2):123-4
11175869
Mol Cell Neurosci. 2001 Mar;17(3):426-43
11273640
J Cereb Blood Flow Metab. 2001 Mar;21(3):211-7
11295875
Diabetes. 2001 May;50(5):1004-11
11334402
Mol Endocrinol. 2001 Sep;15(9):1559-70
11518806
Neurosci Lett. 2002 Jan 25;318(2):53-6
11796184
Nature. 2002 Feb 28;415(6875):1030-4
11875571
Exp Eye Res. 2002 Feb;74(2):231-6
11950233
Nat Med. 2002 Jul;8(7):738-42
12068290
J Neurotrauma. 2002 Jun;19(6):693-703
12165131
J Comp Neurol. 2002 Nov 25;453(4):389-417
12389210
J Diabetes Complications. 2003 Jan-Feb;17(1):29-33
12505754
Am J Physiol Endocrinol Metab. 2003 Aug;285(2):E390-6
12721154
Peptides. 2003 Apr;24(4):611-6
12860206
Proc Natl Acad Sci U S A. 2003 Sep 30;100 Suppl 1:11866-72
12923297
Neuroscientist. 2003 Aug;9(4):261-72
12934709
Nature. 1992 Dec 24-31;360(6406):765-8
1334535
Biochim Biophys Acta. 1992 Aug 17;1132(1):72-4
1380834
Dig Dis Sci. 1992 Nov;37(11):1666-70
1425064
Histochemistry. 1992 Aug;98(1):39-49
1429016
Mol Cell Neurosci. 2003 Sep;24(1):23-40
14550766
J Comp Neurol. 2003 Dec 1;467(1):1-10
14574675
J Mol Endocrinol. 1992 Dec;9(3):265-72
1476614
Neurosci Lett. 2004 Oct 7;369(1):24-7
15380301
Acta Neurol Scand. 2004 Nov;110(5):275-80
15476455
Biochim Biophys Acta. 1990 Aug 27;1050(1-3):69-73
2207170
Anal Biochem. 1987 Apr;162(1):156-9
2440339
Exp Brain Res. 1988;72(2):399-406
2465172
Ann Nutr Metab. 1988;32(5-6):282-8
3076052
J Comp Neurol. 1988 May 22;271(4):519-32
3385016
Histochemistry. 1978 Jun 2;56(1):37-44
350814
Can J Physiol Pharmacol. 1969 Jan;47(1):113-4
5761841
J Comp Neurol. 1965 Jun;124(3):319-35
5861717
Histochemistry. 1982;76(3):341-9
6186646
J Histochem Cytochem. 1983 Jun;31(6):811-7
6188783
Brain Res. 1980 Mar 17;186(1):165-83
6986955
Dev Neurosci. 1995;17(1):1-7
7621745
Brain Res. 1994 Nov 7;663(1):51-60
7850470
Peptides. 1994;15(2):297-302
8008635
Brain Res. 1993 Jan 29;602(1):57-63
8095427
Endocrinology. 1993 Dec;133(6):2861-70
8243312
Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1992-6
8446620
J Neurosci. 1996 Mar 15;16(6):2027-33
8604047
Diabetes. 1996 Sep;45(9):1259-66
8772732
J Clin Invest. 1996 Dec 1;98(11):2440-5
8958204
Science. 1997 Apr 4;276(5309):66-71
9082987
Mol Cell Neurosci. 1997;8(6):389-404
9143557
J Neurosci Res. 1998 Sep 15;53(6):651-62
9753193
Nat Med. 1998 Nov;4(11):1313-7
9809557
Endocr Res. 1998 Aug-Nov;24(3-4):851-6
9888586