title: LmxMPK9, a mitogen-activated protein kinase homologue affects flagellar length in Leishmania mexicana.
abstract: Components of mitogen-activated signal transduction pathways have been shown to be involved in flagellum biogenesis and maintenance. A mitogen-activated protein kinase homologue, designated LmxMPK9 from Leishmania mexicana, has been recently identified in a homology screen and its mRNA found to be present in all life stages. Three different splice-addition sites were used for mRNA maturation in trans-splicing in the different life stages. However, here we show that LmxMPK9 protein is exclusively found in the promastigote stage. Recombinant expression of LmxMPK9 in Escherichia coli and kinase assays revealed a temperature optimum at 27 degrees C, the optimal growth temperature for L. mexicana promastigotes, and a preference for manganese to promote substrate phosphorylation of myelin basic protein. A deletion mutant for the single-copy gene revealed significantly elongated flagella, whereas overexpression led to a subpopulation with rather short to no flagella suggesting a role for LmxMPK9 in flagellar morphogenesis.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Parasitology Section, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Bengs
authors.forename: Anne
authors.initials: A
authors.lastname: Scholz
authors.forename: Daniela
authors.initials: D
authors.lastname: Kuhn
authors.forename: Martin
authors.initials: M
authors.lastname: Wiese
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics; D005407:Flagella / Q000502:physiology* / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000235:genetics; D007894:Leishmania mexicana / Q000201:enzymology* / Q000648:ultrastructure; D020928:Mitogen-Activated Protein Kinases / Q000172:deficiency / Q000502:physiology*; D013696:Temperature
chemical_list: D020928:Mitogen-Activated Protein Kinases