title: Simultaneous determination of active ingredients in Erigeron breviscapus (Vant.) Hand-Mazz. by capillary electrophoresis with electrochemical detection.
abstract: A high-performance capillary electrophoresis (CE) with electrochemical detection (ED) method was developed for the determination of the pharmacologically active ingredients in Erigeron breviscapus (Vant.) Hand-Mazz. and its extract phytopharmaceuticals in this work. Under the optimum conditions, nine analytes, baicalein, naringenin, scopoletin, kaempferol, apigenin, scutellarin, luteolin, caffeic acid and protocatechuic acid were separated within 24 min in a borax buffer (pH 8.7). Notably, excellent linearity was obtained over two orders of magnitude with detection limits (S/N=3) ranged from 1.0 x 10(-7) g/mL to 5.6 x 10(-7) g/mL for all nine analytes. This method was successfully used in the analysis of E. breviscapus (Vant.) Hand-Mazz. and its phytopharmaceuticals with a relatively simple extraction procedure, and the assay results were satisfactory.
authors.affiliation: Department of Chemistry, East China Normal University, 3663 Zhongshan Rd.N, Shanghai 200062, China.
authors.forename: Qingcui
authors.initials: Q
authors.lastname: Chu
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.forename: Liang
authors.initials: L
authors.lastname: Fu
authors.forename: Jiannong
authors.initials: J
authors.lastname: Ye
mesh_terms: D019659:Asteraceae; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D010936:Plant Extracts