Effects of N- and C-terminal truncation of HP (2-20) from Helicobacter pylori ribosomal protein L1 (RPL1) on its anti-microbial activity.
HP (2-20) [derived from the N-terminal region of Helicobacter pylori Ribosomal Protein L1 (RPL1)], a 19-mer peptide, possesses broad-spectrum anti-microbial activity. As the N- (residues 2-3) and C-terminal (residues 14-20) residues can be deleted without affecting antimicrobial activity, we have now determined the minimum chain length necessary for the retention of antimicrobial activity, and its mode of action. The N- (residues 2-3) and C-terminal (residues 17-20) truncated fragments [HP (4-16)] induce increased antibiotic activity against several bacterial strains without hemolysis. Flow cytometric analysis, scanning electron microscopy and fluorescence confocal microscopy revealed that HP (4-16) acted rapidly on the plasma membranes of the fungal cells in a salt- and energy-independent manner.
Park
Yoonkyung
Y
Research Center for Proteineous Materials, Chosun University, 375 Seosuk-Dong, Dong-Ku, Kwangju 501-759, Korea.
Hahm
Kyung-Soo
KS
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001426:Bacterial Proteins / Q000737:chemistry* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects / Q000648:ultrastructure; D002942:Circular Dichroism; D005434:Flow Cytometry; D016480:Helicobacter pylori / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D019810:Sodium Azide / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; C433783:Hp (2-20) protein, Helicobacter pylori; D010446:Peptide Fragments; D012269:Ribosomal Proteins; C028546:ribosomal protein L1; D012965:Sodium Chloride; D019810:Sodium Azide