title: Isolation and characterization of a new simian retrovirus type D subtype from monkeys at the Tsukuba Primate Center, Japan.
abstract: Exogenous type D simian retroviruses (SRV/D) are prevalent in captive and feral populations of various macaque monkeys. Thus far, five subtypes of SRV/Ds have been reported, three of which (SRV-1, -2 and -3) have been molecularly characterized. Two SRV/D strains (N27 and T150) were isolated from seropositive cynomolgus macaques at the Tsukuba Primate Center (TPC) in Japan, showing clinical signs of SRV/D infection, including anemia and persistent unresponsive diarrhea. Electron microscopy demonstrated that both SRV/D isolates have a virion morphology typical of type D retrovirus. The SRV/D N27 and T150 isolates were essentially the same based on sequence analysis. From homology analysis of the entire gag sequence, the N27 isolate is closely related to the other known SRV/Ds but is distinct from the three molecularly characterized SRV/Ds. Thus, we have tentatively designated the N27 and T150 viruses isolated from TPC cynomolgus macaques as SRV/D-Tsukuba (SRV/D-T).
authors.affiliation: Tsukuba Primate Center for Medical Science, National Institute Infectious Diseases, 1 Hachimanndai, Tsukuba 305-0843, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakajima
authors.forename: Akihiko
authors.initials: A
authors.lastname: Uda
authors.forename: Koji
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Tadashi
authors.initials: T
authors.lastname: Baba
authors.forename: Ryozaburo
authors.initials: R
authors.lastname: Mukai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030121:Betaretrovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D015750:Genes, gag; D007564:Japan; D008252:Macaca fascicularis / Q000821:virology*; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D012192:Retroviridae Infections / Q000473:pathology / Q000662:veterinary*; D015301:Retroviruses, Simian / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000473:pathology / Q000662:veterinary*