title: Development of rhinovirus study model using organ culture of turbinate mucosa.
abstract: To better understand the pathophysiology of rhinovirus (RV) infection, a development of a study model using organ culture of turbinate mucosa was sought. Inferior turbinate mucosal tissues were cultured using air-liquid interface methods, on a support of gelfoam soaked in culture media. RV-16 was applied to the mucosal surface and washed off, and histological changes were evaluated. The success of RV infection was assayed by semi-nested RT-PCR of the mucosal surface fluid taken 48h after incubation. Intracellular RVs were visualized by in situ hybridization (ISH). Secretion of the cytokines, IL-6 and IL-8, into the culture media was quantitated by ELISA. After 7 days of culture, the turbinate mucosae did not show significant damage. A PCR product indicating successful RV infection was detected in 5 out of 10 mucosal tissues. ISH showed a very small number of positively stained cells focally located in the epithelial layer. In the beginning 24h after infection, secretion of IL-6 and IL-8 into the culture media of infected mucosae was significantly greater than into the media of control mucosae. Our results indicate that the air-liquid interface organ culture of turbinate mucosa could serve as an acceptable in vitro model for studying RV infection.
authors.affiliation: Department of Otolaryngolgy, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-2dong, Songpa-gu, Seoul 138-736, South Korea. jangyj@amc.seoul.kr
authors.forename: Yong Ju
authors.initials: YJ
authors.lastname: Jang
authors.forename: Si Hyeong
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyon-Ja
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Yoo-Sam
authors.initials: YS
authors.lastname: Chung
authors.forename: Bong-Jae
authors.initials: BJ
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D017403:In Situ Hybridization; D015850:Interleukin-6 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008875:Middle Aged; D009297:Nasal Mucosa / Q000254:growth & development / Q000473:pathology / Q000821:virology*; D009924:Organ Culture Techniques / Q000379:methods*; D010850:Picornaviridae Infections / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012229:Rhinovirus / Q000254:growth & development*; D014420:Turbinates / Q000473:pathology / Q000821:virology*
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D012367:RNA, Viral
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