title: The promoter region rather than its downstream inverted repeat sequence is responsible for low-level transcription of the thermostable direct hemolysin-related hemolysin (trh) gene of Vibrio parahaemolyticus.
abstract: We determined the transcriptional start site of the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by using a PCR-based method and identified the promoter. Mutagenic analysis indicated that the promoter-bearing region rather than its downstream inverted repeat sequence was responsible for the low-revel of trh transcription.
authors.affiliation: Graduate School of Medicine, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Nakaguchi
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Nishibuchi
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000502:physiology*; D011993:Recombinant Fusion Proteins; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000502:physiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D011993:Recombinant Fusion Proteins; C088609:thermostable direct hemolysin-related hemolysin protein, Vibrio parahaemolyticus
references.citation: Infect Immun. 2000 Oct;68(10):5742-8
references.pmid: 10992480
references.citation: Mol Microbiol. 1998 Nov;30(3):499-511
references.pmid: 9822816
references.citation: Lancet. 2003 Mar 1;361(9359):743-9
references.pmid: 12620739
references.citation: Microbiology. 2003 May;149(Pt 5):1217-25
references.pmid: 12724383
references.citation: Microbiol Immunol. 2003;47(3):233-9
references.pmid: 12725294
references.citation: J Mol Biol. 1980 Apr;138(2):179-207
references.pmid: 6997493
references.citation: Nucleic Acids Res. 1988 Aug 25;16(16):8186
references.pmid: 3047679
references.citation: Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002
references.pmid: 2461560
references.citation: Genetics. 1988 Nov;120(3):621-3
references.pmid: 2852134
references.citation: Infect Immun. 1989 Sep;57(9):2691-7
references.pmid: 2759706
references.citation: Mol Microbiol. 1990 Jan;4(1):87-99
references.pmid: 2319944
references.citation: Infect Immun. 1990 Nov;58(11):3568-73
references.pmid: 2228229
references.citation: J Bacteriol. 1990 Nov;172(11):6568-72
references.pmid: 2172217
references.citation: Proc Natl Acad Sci U S A. 1990 Dec;87(24):9898-902
references.pmid: 2124707
references.citation: Nucleic Acids Res. 1991 May 25;19(10):2785
references.pmid: 1645866
references.citation: Microbiol Immunol. 1991;35(3):253-8
references.pmid: 1908042
references.citation: Microb Pathog. 1991 Dec;11(6):453-60
references.pmid: 1795634
references.citation: Appl Environ Microbiol. 1992 Aug;58(8):2449-57
references.pmid: 1514791
references.citation: J Bacteriol. 1993 Jun;175(12):3844-55
references.pmid: 8509337
references.citation: Infect Immun. 1995 Jun;63(6):2093-9
references.pmid: 7768586
references.citation: Biotechniques. 1995 May;18(5):746-50
references.pmid: 7619468
references.citation: Nucleic Acids Res. 1995 Sep 25;23(18):3796-7
references.pmid: 7479016
references.citation: J Bacteriol. 2002 Feb;184(4):936-46
references.pmid: 11807053