Isolation and identification of lactic acid bacteria from soil using an enrichment procedure.
OBJECTIVE
To survey, and identify and classify the ecological distribution of lactic acid bacteria from soil in Japan and Taiwan.


RESULTS
Acid-producing bacteria were isolated from 68 soil samples, collected from Japan and Taiwan, in the rhizospheres of fruit trees, from the floor of a henhouse and around a horse farm. All isolates were identified by physiological and genetic tests. Thirty-two of the 54 isolates were identified as lactic acid bacteria (LAB), 16 as spore-forming lactic acid bacteria, five as Clostridium and one as Bacillus. These lactic acid bacteria represent five genera: Lactobacillus, Lactococcus, Enterococcus, Leuconostoc and Weissella.


CONCLUSIONS
A high rate of isolating lactic acid bacteria was obtained from soil.


CONCLUSIONS
This study suggests that soil may be a common source for the isolation of lactic acid bacteria.
Chen
Y-S
YS
Institute of Enology and Viticulture, Yamanashi University, Kitashin, Kofu, Yamanashi, Japan.
Yanagida
F
F
Shinohara
T
T
D001431:Bacteriological Techniques; D016983:Enterococcus / Q000302:isolation & purification / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism; D017045:Lactococcus / Q000302:isolation & purification / Q000378:metabolism; D007934:Leuconostoc / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid