[Study of preparation methods of polyclonal antibody of chymopapain].
OBJECTIVE
To study the preparation methods of polyclonal antibody of chymopapain with higher titre.


METHODS
Three types of antigens-native chymopapain, chymopapain inactivated by H2O2 and inactivated by iodoacetic acid were injected into different male rabbits by hypodermic injections and by intravenous injection. The blood samples were obtained from veins in ear to test antisera after the 3rd injection, the used methods were Western blotting and ELISA. The data was analyzed by the SAS system.


RESULTS
The antisera titre of two inactivated antigens were obviously higher than that of nature antigen (P < 0.05), while there was no significant difference between the two inactivated antigens. Intravenous injection can obviously increase the ability of immunoresponse and raise antisera titre rapidly.


CONCLUSIONS
The inactivated antigens have better immunogenicity than native chymopapain. As compared with iodoacetic acid, using H2O2 was an economical and effective method. The combination method of hypodermic injections and intravenous injection can raise antisera titre effectively and shorten the period of antibody preparation.
Shu
Wei
W
College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
Guo
Yong
Y
Fang
Shisong
S
Cui
Tangbing
T
D000818:Animals; D000917:Antibody Formation; D015153:Blotting, Western; D002917:Chymopapain / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007106:Immune Sera; D007271:Injections, Intradermal; D007275:Injections, Intravenous; D019807:Iodoacetic Acid / Q000494:pharmacology; D008297:Male; D011817:Rabbits
D007106:Immune Sera; D006861:Hydrogen Peroxide; D002917:Chymopapain; D019807:Iodoacetic Acid