[Protective effects of total flavones of metasequosia on cerebral ischemia-reperfusion injury in rats].
OBJECTIVE
To observe the protective effects of total flavones of metasequosia (TFM) on cerebral ischemia-reperfusion injury in rats.


METHODS
The bilateral vertebral arteries of rats were occluded under anesthesia, and 4-5 h later the carotid arteries of rats in the conscious condition were occluded for 30 min followed by reperfusion for 90 min. The effects of TFM on the contents of water, Na+, Ca2+ in cortex, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in brain hemispheres, as well as the EEG activities were observed.


RESULTS
After 30 min ischemia and 90 min reperfusion, the contents of water, Na+ , Ca2+ and MDA were increased, and the SOD activity was reduced with abnormal EEG activity and ischemic injury in the brain tissues. TFM 25-100 mg x kg(-1) ip 30 min before the carotid arteries were occluded, decreased the elevated water, Na+, Ca2+ and MDA contents, increased the SOD activity, reduced the ischemic injury of brain tissue, and promoted the recovery of EEG activities.


CONCLUSIONS
TFM has a protective effect on cerebral ischemia-reperfusion injury in rats.
Wang
Fang
F
Dept of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, China. yunianlin@sohu.com
Yu
En-xin
EX
Liu
Wei-wan
WW
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D002545:Brain Ischemia / Q000150:complications; D029779:Cupressaceae / Q000737:chemistry; D004569:Electroencephalography / Q000187:drug effects; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology
D047309:Flavones; D018696:Neuroprotective Agents