title: Chemokine receptors in the rheumatoid synovium: upregulation of CXCR5.
abstract: In patients with rheumatoid arthritis (RA), chemokine and chemokine receptor interactions play a central role in the recruitment of leukocytes into inflamed joints. This study was undertaken to characterize the expression of chemokine receptors in the synovial tissue of RA and non-RA patients. RA synovia (n = 8) were obtained from knee joint replacement operations and control non-RA synovia (n = 9) were obtained from arthroscopic knee biopsies sampled from patients with recent meniscal or articular cartilage damage or degeneration. The mRNA expression of chemokine receptors and their ligands was determined using gene microarrays and PCR. The protein expression of these genes was demonstrated by single-label and double-label immunohistochemistry. Microarray analysis showed the mRNA for CXCR5 to be more abundant in RA than non-RA synovial tissue, and of the chemokine receptors studied CXCR5 showed the greatest upregulation. PCR experiments confirmed the differential expression of CXCR5. By immunohistochemistry we were able to detect CXCR5 in all RA and non-RA samples. In the RA samples the presence of CXCR5 was observed on B cells and T cells in the infiltrates but also on macrophages and endothelial cells. In the non-RA samples the presence of CXCR5 was limited to macrophages and endothelial cells. CXCR5 expression in synovial fluid macrophages and peripheral blood monocytes from RA patients was confirmed by PCR. The present study shows that CXCR5 is upregulated in RA synovial tissue and is expressed in a variety of cell types. This receptor may be involved in the recruitment and positioning of B cells, T cells and monocytes/macrophages in the RA synovium. More importantly, the increased level of CXCR5, a homeostatic chemokine receptor, in the RA synovium suggests that non-inflammatory receptor-ligand pairs might play an important role in the pathogenesis of RA.
authors.affiliation: Leopold Muller Arthritis Research Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, UK. Caroline.Schmutz@rjah.nhs.uk
authors.forename: Caroline
authors.initials: C
authors.lastname: Schmutz
authors.forename: Alison
authors.initials: A
authors.lastname: Hulme
authors.forename: Angela
authors.initials: A
authors.lastname: Burman
authors.forename: Mike
authors.initials: M
authors.lastname: Salmon
authors.forename: Brian
authors.initials: B
authors.lastname: Ashton
authors.forename: Christopher
authors.initials: C
authors.lastname: Buckley
authors.forename: Jim
authors.initials: J
authors.lastname: Middleton
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D018951:Antigens, CD20 / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D017252:CD3 Complex / Q000032:analysis; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007717:Knee; D016131:Lymphocyte Subsets / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000235:genetics; D018121:Receptors, Cytokine / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D018951:Antigens, CD20; D017252:CD3 Complex; C095085:CXCR5 protein, human; C513179:CXCR5 protein, mouse; D012333:RNA, Messenger; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine
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