Mitochondrial cytochrome b DNA sequence variations: an approach to fish species identification in processed fish products.
The identification of fish species in food products is problematic because morphological features of the fish are partially or completely lost during processing. It is important to determine fish origin because of the increasing international seafood trade and because European Community Regulation 104/2000 requires that the products be labeled correctly. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. This method allowed the identification of fish species in all samples. Fish in all of the examined products belonged to these two families, with the exception of one sample of smoked baccalà (salt cod), which was not included in the Gadidae cluster.
Pepe
Tiziana
T
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Sezione Ispezione, Universitd degli Studi di Napoli, Federico 11, Via Delpino 1, Napoli 80137, Italy. tiziana.pepe@unina.it
Trotta
Michele
M
di Marco
Isolina
I
Cennamo
Paola
P
Anastasio
Aniello
A
Cortesi
Maria Luisa
ML
D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005396:Fish Products / Q000032:analysis / Q000145:classification; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D005516:Food Microbiology; D005784:Gene Amplification; D010802:Phylogeny; D013045:Species Specificity
D004272:DNA, Mitochondrial; D045303:Cytochromes b