title: The core lipopolysaccharide of Escherichia coli is a ligand for the dendritic-cell-specific intercellular adhesion molecule nonintegrin CD209 receptor.
abstract: The dendritic-cell-specific intercellular adhesion molecule nonintegrin (DC-SIGN) CD209 is a receptor for Escherichia coli K-12 that promotes bacterial adherence and phagocytosis. However, the ligand of E. coli for DC-SIGN has not yet been identified. In this study, we found that DC-SIGN did not mediate the phagocytosis of several pathogenic strains of E. coli, including enteropathogenic E. coli, enterohemorrhagic E. coli, enterotoxigenic E. coli, and uropathogenic E. coli, in dendritic cells or HeLa cells expressing human DC-SIGN antigen. However, we showed that an outer core lipopolysaccharide (LPS) (rough) mutant, unlike an inner core LPS (deep rough) mutant or O-antigen-expressing recombinant of E. coli K-12 was phagocytosed. These results demonstrate that the host cells expressing DC-SIGN can phagocytose E. coli in part by interacting with the complete core region of the LPS molecule. These results provide a mechanism for how O antigen acts as an antiphagocytic factor.
authors.affiliation: School of Molecular Biosciences, Washington State University, Pullman, Washington, USA.
authors.forename: John
authors.initials: J
authors.lastname: Klena
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Olivier
authors.initials: O
authors.lastname: Schwartz
authors.forename: Sheila
authors.initials: S
authors.lastname: Hull
authors.forename: Tie
authors.initials: T
authors.lastname: Chen
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry / Q000502:physiology*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000502:physiology; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000502:physiology*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
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