Micellar electrokinetic chromatography of bilirubin, related compounds, and selected drugs with mixtures of binary bile salts.
Micellar electrokinetic chromatography was used to study the behavior of quinine, propranolol, bilirubin, biliverdin dimethyl ester, and xanthobilirubin methyl ester in single and binary bile salt micelle systems comprised of glycocholic acid and glycodeoxycholic acid. Micelle systems studied had total bile salt concentrations in the range of 10-33 mM with molar ratios of 1:0, 2:1, 1:1, 1:2, and 0:1 glycocholic acid:glycodeoxycholic acid. A pH 8.5 phosphate-borate buffer system was used. For all analytes except bilirubin, the smallest migration factors were found in glycocholic acid solutions and the largest in glycodeoxycholic acid solutions. Intermediate migration factors were found for all compounds except bilirubin in the binary bile salt systems. Bilirubin behaved uniquely with its largest migration factors in the binary bile salt mixtures.
Olivares
Juan
J
Department of Chemistry, Trinity University, San Antonio, TX, USA.
Conner
Matthew J
MJ
Department of Chemistry, Trinity University, San Antonio, TX, USA.
Vass
Christa A
CA
Department of Chemistry, Trinity University, San Antonio, TX, USA.
Kurtin
William E
WE
Department of Chemistry, Trinity University, San Antonio, TX, USA.
Bushey
Michelle M
MM
Department of Chemistry, Trinity University, San Antonio, TX, USA.
D001647:Bile Acids and Salts; D001663:Bilirubin / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006000:Glycocholic Acid / Q000302:isolation & purification; D006002:Glycodeoxycholic Acid / Q000302:isolation & purification; D008823:Micelles
D001647:Bile Acids and Salts; D008823:Micelles; D006002:Glycodeoxycholic Acid; D006000:Glycocholic Acid; D001663:Bilirubin
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