Expression of mRNA encoding interleukin (IL)-10, IL-12p35 and IL-12p40 in lungs from pigs experimentally infected with Actinobacillus pleuropneumoniae.
The expression of mRNA encoding interleukin (IL)-10, IL-12p35 and IL-12p40 was studied, by reverse transcription-polymerase chain reaction and by in situ hybridization with a non-radioactive digoxigenin-labelled cDNA probe, in formalin-fixed, paraffin-wax-embedded lung tissue from pigs experimentally infected with Actinobacillus pleuropneumoniae. Forty-eight 7-week-old colostrum-deprived pigs were randomly allocated to infected (n = 24) or control (n = 24) groups. Three pigs from each group were euthanized at 3, 6, 9, 12, 24, 36, 48 and 60 h post inoculation (hpi). IL-10 mRNA was detected in the lung at 3 hpi, numbers of cells positive for IL-10 increasing at 36 hpi. IL-12p35 mRNA was detected in the lung at 3 hpi, numbers of cells positive for IL-12p35 increasing at 36 and 48 hpi and rapidly decreasing thereafter whereas IL-12p40 mRNA was constitutively expressed at low levels during the experiment. Hybridization signals for IL-10, IL-12p35 and IL-12p40 were always associated with inflammation, in particular with macrophages and neutrophils within alveolar spaces. Expression of these cytokines was minimal in non-lesional lung of A. pleuropneumoniae-infected pigs and in normal lung from control pigs. In situ hybridization of A. pleuropneumoniae and these cytokines in serial sections of lung tissues indicated close co-localization of A. pleuropneumoniae and these cytokines in pleuropneumonia. The results suggest that the expression of IL-10 and IL-12 play a role in pathogenesis of A. pleuropneumoniae infection.
Cho
W S
WS
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Kwanak-Gu 151-742, Seoul, Republic of Korea.
Jung
K
K
Kim
J
J
Ha
Y
Y
Chae
C
C
D000189:Actinobacillus Infections / Q000276:immunology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae; D000818:Animals; D015870:Gene Expression; D017403:In Situ Hybridization / Q000662:veterinary; D016753:Interleukin-10 / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis*; D053765:Interleukin-12 Subunit p35; D053762:Interleukin-12 Subunit p40; D008168:Lung / Q000276:immunology; D011001:Pleuropneumonia / Q000276:immunology / Q000662:veterinary*; D021122:Protein Subunits / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D013997:Time Factors
D053765:Interleukin-12 Subunit p35; D053762:Interleukin-12 Subunit p40; D021122:Protein Subunits; D012333:RNA, Messenger; D016753:Interleukin-10; D018664:Interleukin-12
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