Effects of injecting GABAergic agents into the medullary reticular formation upon swallowing induced by the superior laryngeal nerve stimulation in decerebrate cats.
The purpose of this study was to elucidate the role of the GABAergic system in the medullary reticular formation (MRF) in the control of swallowing. In acutely decerebrated cats (n = 12), swallowing was induced by electrical stimulation (0.3-6 V at 10-20 Hz for 10-20 s every minute) applied to the superior laryngeal nerve (SLN). The stimulus intensity was adjusted so that swallowing was induced two or four times during the period of the stimulation. Bicuculline, a GABA(A) receptor antagonist, was then injected (0.10-0.15 microl, 5 mM) into the MRF through a stereotaxically placed glass micropipette. In a total of 62 injections, 19 injections (30.6%) increased the frequency of SLN-induced swallowing when it was injected into the lateral part of the MRF corresponding to the nucleus reticularis parvocellularis (NRPv). In eight of the effective injections (42.1%) which increased the frequency of SLN-induced swallowing, SLN stimulation also induced coughing. With two injections, stimulation of the SLN-induced coughing but not facilitation of swallowing. On the other hand, an injection of 0.10-0.15 microl of 5 mM muscimol, a GABA(A) receptor agonist, into the NRPv decreased the frequency of SLN-induced swallowing. These results suggest that the NRPv neurons which are responsible for evoking swallowing are under the tonic inhibitory control of the GABAergic system.
Harada
Hirofumi
H
Department of Physiology, Asahikawa Medical College, Midorigaoka-Higashi 2-1-1-1, Asahikawa 078-8510, Japan.
Takakusaki
Kaoru
K
Kita
Shinichi
S
Matsuda
Mitsuyoshi
M
Nonaka
Satoshi
S
Sakamoto
Takashi
T
D000818:Animals; D001640:Bicuculline / Q000008:administration & dosage; D001931:Brain Mapping; D002415:Cats; D003371:Cough / Q000503:physiopathology; D003655:Decerebrate State; D003679:Deglutition / Q000187:drug effects* / Q000502:physiology; D004558:Electric Stimulation; D004576:Electromyography; D018682:GABA Agents / Q000008:administration & dosage*; D005683:Gagging / Q000187:drug effects / Q000502:physiology; D007276:Injections, Intraventricular; D007823:Laryngeal Nerves / Q000502:physiology*; D007830:Larynx / Q000502:physiology; D008845:Microinjections; D009118:Muscimol / Q000008:administration & dosage; D010614:Pharynx / Q000294:innervation / Q000502:physiology; D012154:Reticular Formation / Q000187:drug effects* / Q000502:physiology
D018682:GABA Agents; D009118:Muscimol; D001640:Bicuculline