The effect of the 5-chloro-2-methylthio-6-(1-naphtyloxy)-1H-benzimidazole on the tegument of immature Fasciola hepatica in their natural host.
The damage to the tegument of 3-week-old Fasciola hepatica was evaluated by scanning electron microscopy (SEM) following treatment with the 5-chloro-2-methylthio-6-(1-naphtyloxy)-1H-benzimidazole (called compound alpha) in its natural host. For the present study, flukes were raised in pelibuey sheep infected orally with metacercariae of F. hepatica; the parasites were recovered from the liver of the sacrificed sheep after 6, 12 and 24 h of treatment with compound alpha. At 6 h of treatment, the flukes showed some lesions on the ventral surface of the anterior region, such as a swollen tegument and blebs. At 12 h after treatment, the specimens showed structural disorganization and spine loss in the ventral anterior region. The tegument of the flukes treated for 24 h was completely lost in some areas of the ventral surface, leaving an exposed basal lamina. The tegument of immature F. hepatica might be a target organ for compound alpha to exert its fasciolicide effect.
Rivera
Norma
N
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de Mexico, 04510, Mexico City, Mexico.
Ibarra
Froylán
F
Zepeda
Armando
A
Fortoul
Teresa
T
Cantó
Germinal
G
Hernández
Alicia
A
Castillo
Rafael
R
D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004817:Epidermis / Q000187:drug effects* / Q000648:ultrastructure; D005210:Fasciola hepatica / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D005211:Fascioliasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary; D007093:Imidazoles / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008855:Microscopy, Electron, Scanning; D009281:Naphthalenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology*; D013997:Time Factors
C468032:5-chloro-2-methylthio-6-(1-naphthyloxy)-1H-benzimidazole; D000871:Anthelmintics; D007093:Imidazoles; D009281:Naphthalenes
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