[Effect of natural killer cell on hematopoiesis and immunity recovery in mouse allogeneic bone marrow transplantation].
OBJECTIVE
To study the effect of natural killer (NK)-cell on reconstitution of hematopoiesis and immunity in mouse allogeneic bone marrow transplantation (allo-BMT).


METHODS
Lethally irradiated BALB/c (H-2(d)) mice were transplanted with C57BL/6 (H-2(b)) bone marrow plus peripheral T cells and/or NK cells. Recipients CD34(+) cells and H-2K(b+), CD3(+) and CD19(+) cells were detected by flow cytometry, peripheral white blood cell (WBC) by auto-cytometry, and the survival rates, engraftment, hematopoietic and immune recovery were observed.


RESULTS
In the transplantation with NK cell infusion group, the survival rates, the WBC and CD34(+) cell counts, and the H-2(b+) and CD19(+) cells were significantly higher than that in without NK cell infusion group (P < 0.01). Twenty-eight days after transplantation, the CD3(+) cells in the NK cell infusion group [(33.69 +/- 3.36)%] were lower than that in without [(50.4 +/- 5.06)%] (P < 0.01), and there was no longer difference between these groups 60 days after transplantation (P > 0.05).


CONCLUSIONS
In mouse allo-BMT, alloreactive NK cell enhances engraftment, promotes reconstitution of hematopoiesis and immunity and increases survival rates.
Yang
Zhi-gang
ZG
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
Zeng
Yao-ying
YY
He
Xian-hui
XH
Wang
Qing
Q
Jiang
Xun
X
D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival / Q000276:immunology*; D006410:Hematopoiesis / Q000276:immunology*; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D017710:Lymphocyte Transfusion / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014184:Transplantation, Homologous
D018941:Antigens, CD19; D018952:Antigens, CD34