Modified cellular immune responses in dogs infected with Echinococcus multilocularis.
Parasite-specific antigen responses and lymphocyte blastogenesis in dogs orally inoculated with Echinococcus multilocuralis metacestodes were examined. Serum IgG1 (Th2-oriented) and IgG2 (Th 1-oriented) levels against somatic and excretory-secretory (ES) antigens of protoscoleces and adult worms increased from 7 days post-infection (DPI), with the highest responses against protoscolex excretory-secretory antigen (PES). Specific blastogenesis of peripheral blood mononuclear cells (PBMC) against the parasite antigens was not observed during the 21-day infection period, but Peyer's patches cells from one out of two dogs at 21 DPI showed blastogenesis against PES (stimulation index: 4.65). Interestingly, only at 7 DPI were concanavalin A (ConA)-induce proliferative responses of PBMC reduced. Moreover, ConA-induced proliferative responses of lymphocytes from various origins were suppressed by the addition of parasite antigens, especially with PES. These data suggest that although both Th1- and Th2-oriented humoral immune responses were observed in E. multilocularis infected dogs, the parasite antigens, especially PES, may have incompletely suppressed lymphocyte responses in these dogs.
Kato
Naoko
N
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, 060-0818 Sapporo, Japan. nkato@dokkyomed.ac.jp
Nonaka
Nariaki
N
Oku
Yuzaburo
Y
Kamiya
Masao
M
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D004283:Dog Diseases / Q000276:immunology* / Q000469:parasitology; D004285:Dogs; D004443:Echinococcosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D048210:Echinococcus multilocularis / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D003208:Concanavalin A
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