Identification of the nonA and nonB loci of Bacillus subtilis Marburg permitting the growth of SP10 phage.
Mutational inactivation of both nonA and nonB genes are required for the permissiveness of Bacillus subtilis Marburg cells to infection by phage SP10. By transformational analysis of the nonA strain with DNAs from gently lysed protoplasts carrying the integrative plasmid pMUTIN (em) insertions in every 20 kb along the whole chromosome, we have identified the nonA to be the cured state of endogenous prophage SPbeta. Direct DNA sequencing, on the other hand, revealed one nonsense mutation of nonB in ydiR, which is a component gene of the intrinsic restriction system BsuMR of B.subtilis Marburg. Introduction of the wild type ydiR into the nonB strain at aprE locus resulted in complementation of nonB. Furthermore, as the SP10 genome was found to possess multiple BsuM target sites, it is considered that SP10 can infect and multiply in B.subtilis cells, which are SPbeta free and possess a defective BsuMR restriction system.
Matsuoka
Satoshi
S
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama 338-8570, Japan.
Arai
Tomomi
T
Murayama
Rikinori
R
Kawamura
Fujio
F
Asai
Kei
K
Sadaie
Yoshito
Y
D001412:Bacillus subtilis / Q000235:genetics* / Q000821:virology*; D001435:Bacteriophages / Q000472:pathogenicity*; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics; D039002:Prophages / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial