Characterisation of a cluster of TRIM-B30.2 genes in the chicken MHC B locus.
We have identified and characterised a cluster of six TRIM-B30.2 genes flanking the chicken BF/BL region of the B complex. The TRIM-B30.2 proteins are a subgroup of the TRIM protein family containing the tripartite motif (TRIM), consisting of a RING domain, a B-box and a coiled coil region, and a B30.2-like domain. In humans, a cluster of seven TRIM-B30.2 genes has been characterised within the MHC on Chromosome 6p21.33. Among the six chicken TRIM-B30.2 genes two are orthologous to those of the human MHC, and two (TRIM41 and TRIM7) are orthologous to human genes located on Chromosome 5. In humans, these last two genes are adjacent to GNB2L1, a guanine nucleotide-binding protein gene, the ortholog of the chicken c12.3 gene situated in the vicinity of the TRIM-B30.2 genes. This suggests that breakpoints specific to mammals have occurred and led to the remodelling of their MHC structure. In terms of structure, like their mammalian counterparts, each chicken gene consists of five coding exons; exon 1 encodes the RING domain and the B-box, exons 2, 3 and 4 form the coiled-coil region, and the last exon represents the B30.2-like domain. Phylogenetic analysis led us to assume that this extended BF/BL region may be similar to the human extended class I region, because it contains a cluster of BG genes sharing an Ig-V like domain with the BTN genes (Henry et al. 1997a) and six TRIM-B30.2 genes containing the B30.2-like domain, shared with the TRIM-B30.2 members and the BTN genes.
Ruby
Thomas
T
UPR 1983, CNRS, 7 rue Guy Môquet, 94801, Villejuif Cedex, France.
Bed'Hom
Bertrand
B
Wittzell
Hakan
H
Morin
Véronique
V
Oudin
Anne
A
Zoorob
Rima
R
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000235:genetics*; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017433:Protein Structure, Secondary; D016415:Sequence Alignment
D015395:Histocompatibility Antigens Class I
J Mol Evol. 1993 Dec;37(6):600-12
8114113
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3958-62
8632997
Trends Biochem Sci. 1992 Sep;17(9):344-5
1412709
Nature. 2004 Feb 26;427(6977):848-53
14985764
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8
3162770
Nat Genet. 2000 Jul;25(3):298-301
10888877
Scand J Immunol. 1981 Dec;14(6):607-16
7043723
J Hered. 1999 Jan-Feb;90(1):152-9
9987924
Mol Biol Evol. 1998 Dec;15(12):1696-705
9866204
Science. 1981 Sep 25;213(4515):1469-74
6792704
Immunol Rev. 1999 Feb;167:33-44
10319249
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4594-8
2499885
Genomics. 2001 Feb 1;71(3):351-62
11170752
Immunol Rev. 1995 Feb;143:63-88
7558083
Immunogenetics. 2000 Apr;51(4-5):373-82
10803852
Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1941-4
8700863
Biochem Cell Biol. 1998;76(2-3):351-8
9923704
Immunogenetics. 1993;37(6):408-14
8436415
Nat Genet. 1997 Nov;17(3):285-91
9354791
Immunogenetics. 1997;46(5):383-95
9271628
Biochem Biophys Res Commun. 1997 Jun 9;235(1):162-5
9196055
Avian Dis. 1992 Apr-Jun;36(2):378-85
1320867
Tissue Antigens. 2003 Jan;61(1):63-71
12622776
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Biochem Biophys Res Commun. 1998 Oct 20;251(2):515-9
9792805
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1359-63
1996336
Prog Clin Biol Res. 1987;238:219-33
3037561
Cytogenet Cell Genet. 1996;75(1):7-9
8995478
Genetics. 1948 Nov;33(6):605
18208304
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4377-81
1903541
Bioinformatics. 2001 Dec;17(12):1244-5
11751241
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2550-4
1826056
J Virol. 2002 Apr;76(8):3810-8
11907221
EMBO J. 1988 Sep;7(9):2775-85
3141149
Immunogenetics. 1990;32(1):45-50
2373526
Poult Sci. 1982 Feb;61(2):205-11
7088788
J Virol. 2003 Jun;77(12 ):7101-5
12768029
Science. 1977 Jan 14;195(4274):193-5
831269
Oncogene. 1999 Feb 4;18(5):1209-17
10022127
Mol Cell Biol. 1988 Apr;8(4):1853-6
3380101
Biochem Biophys Res Commun. 2000 Sep 16;276(1):45-51
11006080
EMBO J. 2001 May 1;20(9):2140-51
11331580
Nature. 1999 Oct 28;401(6756):923-5
10553909
Nature. 2003 Oct 23;425(6960):805-11
14574404