The first crystal structure of a macromolecular assembly under high pressure: CpMV at 330 MPa.
The structure of cubic Cowpea mosaic virus crystals, compressed at 330 MPa in a diamond anvil cell, was refined at 2.8 A from data collected using ultrashort-wavelength (0.331 A) synchrotron radiation. With respect to the structure at atmospheric pressure, order is increased with lower Debye Waller factors and a larger number of ordered water molecules. Hydrogen-bond lengths are on average shorter and the cavity volume is strongly reduced. A tentative mechanistic explanation is given for the coexistence of disordered and ordered cubic crystals in crystallization drops and for the disorder-order transition observed in disordered crystals submitted to high pressure. Based on such explanation, it can be concluded that pressure would in general improve, albeit to a variable extent, the order in macromolecular crystals.
Girard
Eric
E
Synchrotron SOLEIL, Gif sur Yvette, France.
Kahn
Richard
R
Mezouar
Mohamed
M
Dhaussy
Anne-Claire
AC
Lin
Tianwei
T
Johnson
John E
JE
Fourme
Roger
R
D001703:Biophysics / Q000379:methods*; D002213:Capsid / Q000737:chemistry*; D017798:Comovirus / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D011312:Pressure; D011506:Proteins / Q000737:chemistry; D012984:Software; D013499:Surface Properties; D017356:Synchrotrons; D013696:Temperature; D014867:Water
D046911:Macromolecular Substances; D011506:Proteins; D014867:Water
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