[Reversed-phase high performance liquid chromatographic (RP-HPLC) analysis of taxol and related taxanes extracted from Taxus wallichiana zucc].
A method for determination of taxol, 10-deacetyl-7-epitaxol, cephalomannine, 10-deacetyl taxol, baccatin III and 10-deacetylbaccatin III, is reported and their retention mechanism is also discussed. These compounds were separated well by RP-HPLC on a Resolvepak C18 column with methanol-water-phosphoric acid (60:40:0.5) as mobile phase and UV detector at 228nm. The powdered stems were extracted with 95% ethanol, refluxed for 8 hrs and filtered and then the residue was partitioned three times between water and chloroform. The organic phase was evaporated to dryness and the residue dissolved in reagent methanol and was submitted to HPLC analysis. The results showed that there is a linear relationship between the peak area and the amount of these compounds over the range of 0.1-2.0microg. The lowest detectable concentration of these compounds was below 0.1microg.
Wang
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Research Institute of Forest Ecology and Environment, Chinese Academy of Forestry, Beijing, 100091.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017239:Paclitaxel / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D043823:Taxoids / Q000032:analysis*; D020946:Taxus / Q000737:chemistry*
D010936:Plant Extracts; D043823:Taxoids; D017239:Paclitaxel