title: A qualitative study of compact bone microstructure and nuclear short tandem repeat obtained from femur of human remains found on the ground and exhumed 3 years after death.
abstract: Forensic identification of human remains is composed of anthropological study of race, sex, age, etc. By using these traditional methods, inconclusive or nonidentified cases could be subjected to DNA analysis. However, in spite of advances in human identification techniques, especially by PCR-amplified DNA, some limitations that affect the ability of obtaining DNA from human remains still persist. Light microscope sections of postmortem compact bones from human remains are presented here for the purpose of increasing a forensic examiner's prediction of successful nuclear DNA typing. Femoral compact bones were obtained from 7 human remains found on the ground, in different degrees of decomposition, and were cleaned by boiling to remove soft tissues, 8 collections of bones having undergone natural decomposition, not boiled (as no soft tissue was adhered), and 5 cadavers 12 to 16 hours postmortem. The histologic sections were stained by hematoxylin and eosin, the loci CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317, and amelogenin were amplified by PCR, and the polyacrylamide gel was stained with silver. The results presented here clarify questions concerning the viability of DNA for identification analysis, and they also may help to establish better strategies for optimization of DNA extraction and analysis in compact bones of human remains.
authors.affiliation: Departamento de Medicina Legal, Etica Médica, Medicina Social e do Trabalho, Instituto Oscar Freire, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. eiwamura@usp.br
authors.forename: Edna S Miazato
authors.initials: ES
authors.lastname: Iwamura
authors.forename: Claudia Regina G C M
authors.initials: CR
authors.lastname: Oliveira
authors.forename: José Arnaldo
authors.initials: JA
authors.lastname: Soares-Vieira
authors.forename: Sergio A B
authors.initials: SA
authors.lastname: Nascimento
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Muñoz
mesh_terms: D000328:Adult; D000368:Aged; D002102:Cadaver; D016172:DNA Fingerprinting / Q000379:methods; D049249:Exhumation; D005260:Female; D005269:Femur / Q000473:pathology*; D005554:Forensic Medicine / Q000379:methods; D005787:Gene Frequency; D049668:History, 15th Century; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D020080:Tandem Repeat Sequences