Transient RNAi induction against endogenous genes in Arabidopsis protoplasts using in vitro-prepared double-stranded RNA.
RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes.
An
Chung-Il
CI
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
Sawada
Aki
A
Kawaguchi
Yoshiaki
Y
Fukusaki
Ei-ichiro
E
Kobayashi
Akio
A
D017360:Arabidopsis / Q000235:genetics*; D002478:Cells, Cultured; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D011523:Protoplasts / Q000502:physiology*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000502:physiology*; D013997:Time Factors
D012330:RNA, Double-Stranded