title: Metal induced selectivity in phosphate ion binding in E9 DNase.
abstract: Mass spectrometric and calorimetric data reveal that phosphate ion binding to the active site of colicin E9 DNase is delicately regulated by concomitant binding of specific transition metal ions.
authors.affiliation: Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research & Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
authors.forename: Ewald T J
authors.initials: ET
authors.lastname: van den Bremer
authors.forename: Anthony H
authors.initials: AH
authors.lastname: Keeble
authors.forename: Colin
authors.initials: C
authors.lastname: Kleanthous
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
mesh_terms: D001665:Binding Sites; D002151:Calorimetry; D018360:Crystallography, X-Ray; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D008670:Metals / Q000737:chemistry*; D015394:Molecular Structure; D010710:Phosphates / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D029968:Escherichia coli Proteins; D008670:Metals; D010710:Phosphates; D003851:Deoxyribonucleases