Ultrasonic extraction followed by sulfuric acid silica gel cleanup for the determination of alpha-hexachlorocyclohexane enantiomers in biota samples.
Interest in the analysis of alpha-hexachlorocyclohexane (alpha-HCH) enantiomers as an alternative or complementary approach to elucidating isomer ratios of alpha/gamma-HCH has grown in recent years because it can provide useful information to evaluate the influence of different degradation and transformation processes. In this paper, a simple and rapid method for the determination of alpha-HCH enantiomers in biota samples is described. The method developed consists of ultrasonic extraction, sulfuric acid silica gel cleanup, solid-phase extraction (SPE) column fractionation, and final determination with chiral high-resolution gas chromatography. Ultrasonic extraction greatly shortens the extraction process time, and the sulfuric acid silica gel and SPE cleanup perfectly remove lipids and other interference compounds in the lipid-rich samples. The method is found to be simple, less time-consuming, and easy to operate, thus providing a useful alternative method to assess the enantioselective breakdown of alpha-HCH in biota system.
Zhao
Rusong
R
Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, P.R. China
Chu
Shaogang
S
Zhao
Rongbiao
R
Xu
Xiaobai
X
Liu
Xiufen
X
D044822:Biodiversity; D002850:Chromatography, Gel / Q000379:methods; D001556:Hexachlorocyclohexane / Q000032:analysis*; D010575:Pesticides / Q000032:analysis; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D013464:Sulfuric Acids / Q000737:chemistry* / Q000145:classification*; D014465:Ultrasonics
D010575:Pesticides; D013464:Sulfuric Acids; C040534:alpha-hexachlorocyclohexane; D001556:Hexachlorocyclohexane; D058428:Silica Gel; D012822:Silicon Dioxide; C033158:sulfuric acid