A novel method for making "tissue" microarrays from small numbers of suspension cells.
Tissue microarrays (TMAs) are a highly efficient method for large-scale protein expression studies. To date most TMAs have been constructed using paraffin-embedded specimens. The authors developed a method that allows construction of TMAs from small numbers of cells in suspension. Spun pellets of 1x10 to 1x10 cells are directly processed and embedded in paraffin in an Eppendorf tube. Cylindrical cores of 0.6 mm are taken from these tubes and embedded in a recipient paraffin block to create a TMA. This relatively simple but versatile method enables very small numbers of cells in suspension to be analyzed using the TMA technology and allows for the study of hematolymphoid and related disorders of the blood and bone marrow for which solid tissue samples cannot be readily obtained. With the increasing trend toward obtaining small samples for screening and diagnostic purposes, this method provides a means to manipulate small volume samples for high-throughput immunohistochemical analysis. This method is also amenable for use for cultured cells.
Montgomery
Kelli
K
Department of Pathology, Stanford University Medical Center, Stanford, CA 94305, USA.
Zhao
Shuchun
S
van de Rijn
Matt
M
Natkunam
Yasodha
Y
D001854:Bone Marrow Cells / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D006402:Hematologic Diseases / Q000235:genetics / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008206:Lymphatic Diseases / Q000235:genetics / Q000473:pathology; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000378:metabolism*
D011506:Proteins