title: Nucleotide sequence of the head assembly gene cluster of bacteriophage L and decoration protein characterization.
abstract: The temperate Salmonella enterica bacteriophage L is a close relative of the very well studied bacteriophage P22. In this study we show that the L procapsid assembly and DNA packaging genes, which encode terminase, portal, scaffold, and coat proteins, are extremely close relatives of the homologous P22 genes (96.3 to 99.1% identity in encoded amino acid sequence). However, we also identify an L gene, dec, which is not present in the P22 genome and which encodes a protein (Dec) that is present on the surface of L virions in about 150 to 180 molecules/virion. We also show that the Dec protein is a trimer in solution and that it binds to P22 virions in numbers similar to those for L virions. Its binding dramatically stabilizes P22 virions against disruption by a magnesium ion chelating agent. Dec protein binds to P22 coat protein shells that have expanded naturally in vivo or by sodium dodecyl sulfate treatment in vitro but does not bind to unexpanded procapsid shells. Finally, analysis of phage L restriction site locations and a number of patches of nucleotide sequence suggest that phages ST64T and L are extremely close relatives, perhaps the two closest relatives that have been independently isolated to date among the lambdoid phages.
authors.affiliation: Division of Cell Biology and Immunology, Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA.
authors.forename: Eddie B
authors.initials: EB
authors.lastname: Gilcrease
authors.forename: Danella A
authors.initials: DA
authors.lastname: Winn-Stapley
authors.forename: F Curtis
authors.initials: FC
authors.lastname: Hewitt
authors.forename: Lisa
authors.initials: L
authors.lastname: Joss
authors.forename: Sherwood R
authors.initials: SR
authors.lastname: Casjens
mesh_terms: D017100:Bacteriophage P22 / Q000235:genetics / Q000378:metabolism; D001435:Bacteriophages / Q000235:genetics* / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D005814:Genes, Viral / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D019779:Salmonella enterica / Q000821:virology; D014771:Virion / Q000235:genetics
chemical_list: D036022:Capsid Proteins
references.citation: Virology. 1970 Mar;40(3):505-13
references.pmid: 4908729
references.citation: Virology. 1971 Sep;45(3):638-52
references.pmid: 4942206
references.citation: Mol Gen Genet. 1971;113(4):363-6
references.pmid: 5144631
references.citation: J Mol Biol. 1973 Nov 15;80(4):669-95
references.pmid: 4773026
references.citation: J Supramol Struct. 1974;2(2-4):202-24
references.pmid: 4612247
references.citation: J Mol Biol. 1974 Sep 15;88(2):535-45
references.pmid: 4476800
references.citation: Biochemistry. 1964 Mar;3:297-317
references.pmid: 14155091
references.citation: J Bacteriol. 2005 Feb;187(3):1091-104
references.pmid: 15659686
references.citation: J Mol Biol. 1975 Jan 15;91(2):187-99
references.pmid: 1102701
references.citation: Virology. 1975 Dec;68(2):525-33
references.pmid: 1105961
references.citation: J Mol Biol. 1976 Jun 25;104(2):387-410
references.pmid: 781287
references.citation: J Mol Biol. 1977 Feb 5;109(4):487-514
references.pmid: 15127
references.citation: J Mol Biol. 1977 Feb 5;109(4):515-37
references.pmid: 845938
references.citation: J Virol. 1977 Oct;24(1):363-77
references.pmid: 409854
references.citation: J Mol Biol. 1977 Dec 15;117(3):733-59
references.pmid: 609100
references.citation: J Mol Biol. 1978 Apr 25;120(4):533-44
references.pmid: 650689
references.citation: Microbiol Rev. 1978 Jun;42(2):385-413
references.pmid: 353481
references.citation: Virology. 1978 Sep;89(2):618-22
references.pmid: 716221
references.citation: J Bacteriol. 1979 Jan;137(1):433-9
references.pmid: 368021
references.citation: J Mol Biol. 1979 Jun 15;131(1):1-14
references.pmid: 385887
references.citation: J Virol. 1979 Oct;32(1):1-7
references.pmid: 396381
references.citation: Cell. 1980 Sep;21(2):319-31
references.pmid: 6447542
references.citation: Virology. 1981 Oct 15;114(1):161-74
references.pmid: 6456593
references.citation: Biophys J. 1981 Dec;36(3):575-88
references.pmid: 7326325
references.citation: Adv Biophys. 1984;17:97-146
references.pmid: 6399817
references.citation: J Virol. 1985 Dec;56(3):1034-6
references.pmid: 4068141
references.citation: Virology. 1985 Dec;147(2):431-40
references.pmid: 3000073
references.citation: Eur J Biochem. 1986 Jan 2;154(1):193-6
references.pmid: 3080313
references.citation: J Mol Biol. 1988 Feb 5;199(3):467-74
references.pmid: 3280806
references.citation: Mol Biol Evol. 1985 Nov;2(6):539-56
references.pmid: 3870876
references.citation: Virology. 1989 Aug;171(2):588-98
references.pmid: 2763468
references.citation: Mol Biol Evol. 1989 Sep;6(5):526-38
references.pmid: 2677599
references.citation: EMBO J. 1989 Dec 1;8(12):3563-70
references.pmid: 2583109
references.citation: Methods Enzymol. 1990;185:60-89
references.pmid: 2199796
references.citation: Virology. 1991 Aug;183(2):519-38
references.pmid: 1853558
references.citation: J Mol Biol. 1993 May 5;231(1):65-74
references.pmid: 8496966
references.citation: J Mol Biol. 1993 Oct 20;233(4):682-94
references.pmid: 8411174
references.citation: Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5652-6
references.pmid: 8202543
references.citation: Methods Mol Biol. 1994;25:181-94
references.pmid: 8004164
references.citation: Methods Enzymol. 1995;259:427-52
references.pmid: 8538465
references.citation: J Mol Biol. 1996 Nov 1;263(3):447-62
references.pmid: 8918600
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Gene. 1997 Aug 11;195(1):93-100
references.pmid: 9300826
references.citation: J Virol. 1998 May;72(5):3944-51
references.pmid: 9557680
references.citation: Gene. 1998 Jul 30;215(2):439-44
references.pmid: 9714843
references.citation: Cell. 1998 Oct 30;95(3):431-7
references.pmid: 9814712
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2192-7
references.pmid: 10051617
references.citation: J Virol. 2000 Feb;74(4):1663-73
references.pmid: 10644336
references.citation: Nat Struct Biol. 2000 Mar;7(3):230-7
references.pmid: 10700283
references.citation: Virology. 2000 Jun 5;271(2):321-33
references.pmid: 10860886
references.citation: Virology. 2001 Jan 20;279(2):385-91
references.pmid: 11162794
references.citation: J Virol. 2001 Aug;75(15):7131-41
references.pmid: 11435594
references.citation: Nature. 2001 Oct 25;413(6858):852-6
references.pmid: 11677609
references.citation: J Bacteriol. 2002 Nov;184(22):6235-49
references.pmid: 12399494
references.citation: J Bacteriol. 2003 Jun;185(11):3473-5
references.pmid: 12754248
references.citation: Mol Ther. 2004 Apr;9(4):617-24
references.pmid: 15093192
references.citation: Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6003-8
references.pmid: 15071181
references.citation: J Mol Biol. 2004 May 28;339(2):379-94
references.pmid: 15136040
references.citation: Virology. 2004 Aug 15;326(1):41-6
references.pmid: 15262493
references.citation: Virology. 1967 Feb;31(2):272-8
references.pmid: 5336337
references.citation: Virology. 1968 Jun;35(2):238-47
references.pmid: 4875829
references.citation: J Virol. 1999 Aug;73(8):6821-30
references.pmid: 10400780