title: Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis.
abstract: * Intergeneric hybrids between Lolium multiflorum and Festuca pratensis (Lm/Fp) and their derivatives exhibit a unique combination of genetic and cytogenetic characteristics: chromosomes undergo a high frequency of homoeologous recombination at meiosis; the chromosomes of the two species can easily be discriminated by genomic in situ hybridization (GISH); recombination occurs along the entire length of homoeologous bivalents; a high frequency of marker polymorphism is observed between the two species. * This combination of characters has been used to transfer and isolate a F. pratensis chromosome segment carrying a mutant 'stay-green' gene conferring a disrupted leaf senescence phenotype into L. multiflorum. * The genetic location within the introgressed F. pratensis segment of the senescence gene has been mapped using amplified fragment length polymorphisms (AFLPs), and F. pratensis-specific AFLP markers closely flanking the green gene have been cloned. * The use of these cloned sequences as markers for the stay-green locus in marker-assisted selection programmes has been tested. The potential application of Lm/Fp introgressions as a tool for the map-based cloning of introgressed Fp genes is discussed.
authors.affiliation: Molecular and Applied Genetics Team, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, SY23 3EB, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Moore
authors.forename: I S
authors.initials: IS
authors.lastname: Donnison
authors.forename: J A
authors.initials: JA
authors.lastname: Harper
authors.forename: I P
authors.initials: IP
authors.lastname: Armstead
authors.forename: J
authors.initials: J
authors.lastname: King
authors.forename: H
authors.initials: H
authors.lastname: Thomas
authors.forename: R N
authors.initials: RN
authors.lastname: Jones
authors.forename: T H
authors.initials: TH
authors.lastname: Jones
authors.forename: H M
authors.initials: HM
authors.lastname: Thomas
authors.forename: W G
authors.initials: WG
authors.lastname: Morgan
authors.forename: A
authors.initials: A
authors.lastname: Thomas
authors.forename: H J
authors.initials: HJ
authors.lastname: Ougham
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Fentem
authors.forename: L A
authors.initials: LA
authors.lastname: Roberts
authors.forename: I P
authors.initials: IP
authors.lastname: King
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D031751:Festuca / Q000235:genetics* / Q000502:physiology; D017343:Genes, Plant; D008040:Genetic Linkage; D008129:Lolium / Q000235:genetics; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D010641:Phenotype; D011110:Polymorphism, Genetic