title: Isolation of virus from a SARS patient and genome-wide analysis of genetic mutations related to pathogenesis and epidemiology from 47 SARS-CoV isolates.
abstract: Severe acute respiratory syndrome (SARS) caused by SARS-associated coronavirus (SARS-CoV) is a fatal disease. Prevention of future outbreaks is essential and requires understanding pathogenesis and evolution of the virus. We have isolated a SARS-CoV in China and analyzed 47 SARS-CoV genomes with the aims to reveal the evolution trends of the virus and provide insights into understanding pathogenesis and SARS epidemic. Specimen from a SARS patient was inoculated into cell culture. The presence of SARS-CoV was determined by RT-PCR and confirmed by electron microscopy. Virus was isolated followed by the determination of its genome sequences, which were then analyzed by comparing with other 46 SARS-CoV genomes. Genetic mutations with potential implications to pathogenesis and the epidemic were characterized. This viral genome consists of 29,728 nucleotides with overall organization in agreement with that of published isolates. A total of 348 positions were mutated on 47 viral genomes. Among them 22 had mutations in more than three genomes. Hot spots of nucleotide variations and unique trends of mutations were identified on the viral genomes. Mutation rates were different from gene to gene and were correlated well with periodical or geographic characteristics of the epidemic.
authors.affiliation: Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Mo
authors.initials: M
authors.lastname: Liu
authors.forename: Weiguang
authors.initials: W
authors.lastname: Zhao
authors.forename: Jianlin
authors.initials: J
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Chunfang
authors.initials: C
authors.lastname: Gu
authors.forename: Kailang
authors.initials: K
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Congyi
authors.initials: C
authors.lastname: Zheng
authors.forename: Gengfu
authors.initials: G
authors.lastname: Xiao
authors.forename: Huimin
authors.initials: H
authors.lastname: Yan
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
authors.forename: Deyin
authors.initials: D
authors.lastname: Guo
authors.forename: Po
authors.initials: P
authors.lastname: Tien
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D002681:China / Q000453:epidemiology; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D019143:Evolution, Molecular; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009154:Mutation; D020125:Mutation, Missense; D017354:Point Mutation; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D017421:Sequence Analysis; D045169:Severe Acute Respiratory Syndrome / Q000453:epidemiology / Q000821:virology*; D014774:Virulence / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral
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