[Study on cardiac gene expression microarray and recipe for activating blood circulation and supplementing qi on heart of rats with post-infarction heart failure].
OBJECTIVE
To observe the effect of recipe for activating blood circulation and supplementing Qi (RAS) on cardiac functional structure in rats with post-infarction heart failure (PIHF).


METHODS
Rat model of PIHF was established by left coronary artery ligation. Left ventricular samples of model rats from infarcted or peri-infarcted area were obtained at PIHF formation stage and stable stage (10 days and 8 weeks respectively after operation), the total RNA extracted and detected using 6 pieces of rat's 40s gene microarray (4096 genes/microarray), the data were analyzed using software as Genespring, Treeview, Clustering and SOM. Besides, RAS was used to treat the model rats beginning from 4 weeks after modeling and lasted for 4 weeks, changes of heart function and cardiac coefficient before and after treatment were observed by impedance method with Captopril as positive control.


RESULTS
(1) Genespring analysis showed thousands of genes differential expression (upper or down regulated), including 13 kinds of gene involving energy metabolism, myocardial cytoskeleton, fibrosis, etc. which, in the infarcted area at heart formation stage were 1086 genes and at the stable stage, 724 genes, while in the peri-infarcted area, formation stage 196 genes and stable stage 97 genes. (2) After RAS or Captopril treatment, the heart function improved significantly, with the stroke volume, cardiac output and cardiac index increased significantly (P < 0.01). RAS could also improve the cardiac coefficient of model rats, as compared with that in untreated model, P < 0.01, compared with that in the sham-operated rats, P < 0.05.


CONCLUSIONS
PIHF is a kind of overload heart disease with multiple genes abnormality. RAS could improve the heart function and histologic indexes, so as to treat the heart failure.
Chen
Jin-xing
JX
Key Lab of Ministry of Education on Internal Medicine of TCM, Dongzhimen Hospital of Beijing CM University, Beijing 100700.
Wang
Shuo-ren
SR
Zhu
Ling-qun
LQ
Zhao
Ming-jing
MJ
Lu
Xi-ying
XY
Qin
Ying
Y
Zhang
Zhuang
Z
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression; D006333:Heart Failure / Q000209:etiology / Q000235:genetics / Q000503:physiopathology*; D009203:Myocardial Infarction / Q000150:complications / Q000235:genetics / Q000503:physiopathology*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal