Molecular characterization and chromosomal localization of female-specific genes from the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae).
We report here the molecular characterization of the female-specific FST (female-specific transcript) genes from the Mediterranean fruit fly (medfly) Ceratitis capitata. A genomic clone was isolated, containing a sequence coding for FST. Nucleotide analysis of the clone showed that the gene contains a putative unique intron located in the region encoding the signal peptide. Southern blotting and in situ hybridization analysis on polytene chromosomes suggested the presence of additional genes similar to FST in the genome of the medfly. A novel cDNA clone was isolated from an accessory gland cDNA library, encoding a product that shares 98% identity with the hypothetical translational product of the previously isolated FST cDNA. The novel cDNA was therefore named FST2. The analysis of mitotic and polytene chromosomes by in situ hybridization showed that FST genes map on the left arm of the 4th chromosome of C. capitata.
Ciolfi
Silvia
S
Department of Evolutionary Biology, University of Siena, Italy.
de Filippis
Tiziana
T
Torti
Cristina
C
Malacrida
Anna R
AR
Dallai
Romano
R
D000818:Animals; D001483:Base Sequence; D033622:Ceratitis capitata / Q000235:genetics*; D005260:Female; D015723:Gene Library; D017344:Genes, Insect / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012737:Sex Factors