Accumulation of miR-155 and BIC RNA in human B cell lymphomas.
We show that the microRNA miR-155 can be processed from sequences present in BIC RNA, a spliced and polyadenylated but non-protein-coding RNA that accumulates in lymphoma cells. The precursor of miR-155 is likely a transient spliced or unspliced nuclear BIC transcript rather than accumulated BIC RNA, which is primarily cytoplasmic. By using a sensitive and quantitative assay, we find that clinical isolates of several types of B cell lymphomas, including diffuse large B cell lymphoma (DLBCL), have 10- to 30-fold higher copy numbers of miR-155 than do normal circulating B cells. Similarly, the quantities of BIC RNA are elevated in lymphoma cells, but ratios of the amounts of the two RNAs are not constant, suggesting that the level of miR-155 is controlled by transcription and processing. Significantly higher levels of miR-155 are present in DLBCLs with an activated B cell phenotype than with the germinal center phenotype. Because patients with activated B cell-type DLBCL have a poorer clinical prognosis, quantification of this microRNA may be diagnostically useful.
Eis
Peggy S
PS
Department of Biomolecular Chemistry, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706, USA.
Tam
Wayne
W
Sun
Liping
L
Chadburn
Amy
A
Li
Zongdong
Z
Gomez
Mario F
MF
Lund
Elsebet
E
Dahlberg
James E
JE
D045744:Cell Line, Tumor; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D035683:MicroRNAs / Q000032:analysis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000032:analysis / Q000378:metabolism*
D035683:MicroRNAs; D012333:RNA, Messenger; D022661:RNA, Untranslated
Mol Cell. 2004 Jul 23;15(2):185-97
15260970
Curr Biol. 2002 Apr 30;12(9):735-9
12007417
Mol Cancer Res. 2003 Oct;1(12):882-91
14573789
N Engl J Med. 2004 Apr 29;350(18):1828-37
15115829
Genome Res. 2004 Oct;14(10A):1902-10
15364901
RNA. 2004 Oct;10(10):1586-94
15337850
Science. 2004 Jan 2;303(5654):83-6
14657504
Gene. 2001 Aug 22;274(1-2):157-67
11675008
Cell. 2004 May 28;117(5):663-76
15163413
Mol Cell. 2004 Dec 22;16(6):861-5
15610730
Cell. 2004 Jan 23;116(2):281-97
14744438
Semin Cancer Biol. 2003 Aug;13(4):253-8
14563119
RNA. 2003 Jan;9(1):112-23
12554881
Genome Biol. 2003;4(7):403
12844357
PLoS Biol. 2004 Nov;2(11):e363
15502875
Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11755-60
15284443
Curr Opin Oncol. 2004 Sep;16(5):436-41
15314511
RNA. 2004 Aug;10(8):1174-7
15272117
Mol Cell Biol. 1997 Mar;17(3):1490-502
9032277
J Pathol. 2004 Jan;202(1):60-9
14694522
Exp Cell Res. 2004 May 15;296(1):12-20
15120988
Am J Surg Pathol. 2004 Apr;28(4):464-70
15087665
Oncogene. 2002 Jul 25;21(32):4908-20
12118370
Cancer Res. 2004 Jun 1;64(11):3753-6
15172979
Trends Genet. 2004 Dec;20(12):617-24
15522457
Blood. 2002 Apr 15;99(8):3060-2
11929801
Trends Genet. 2002 Apr;18(4):171-3
11932009
Nat Biotechnol. 2001 Jul;19(7):673-6
11433281
Mol Cell Biol. 1989 Jun;9(6):2657-64
2548084
J Virol. 2002 May;76(9):4275-86
11932393
Nat Rev Genet. 2004 Jul;5(7):522-31
15211354
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15524-9
12434020
Dev Biol. 2004 Jun 15;270(2):488-98
15183728
Genes Chromosomes Cancer. 2004 Feb;39(2):167-9
14695998
RNA. 2004 Jul;10(7):1153-61
15208450
Cell Immunol. 2002 May-Jun;217(1-2):78-86
12426003
Genes Chromosomes Cancer. 2003 May;37(1):20-8
12661002
Anal Biochem. 1987 Apr;162(1):156-9
2440339