title: Mouse ovary developmental RNA and protein markers from gene expression profiling.
abstract: To identify genes involved in morphogenetic events during mouse ovary development, we started with microarray analyses of whole organ RNA. Transcripts for 60% of the 15,000 gene NIA panel were detected, and about 2000 were differentially expressed in nascent newborn compared to adult ovary. Highly differentially expressed transcripts included noncoding RNAs and newly detected genes involved in transcription regulation and signal transduction. The phased pattern of newborn mouse ovary differentiation allowed us to (1) extend information on activity and stage specificity of cell type-specific genes; and (2) generate a list of candidate genes involved in primordial follicle formation, including podocalyxin (Podxl), PDGFR-beta, and a follistatin-domain-encoding gene Flst1. Oocyte-specific transcripts included many (e.g., Deltex2, Bicd2, and Zfp37) enriched in growing oocytes, as well as a novel family of untranslated RNA's (RLTR10) that is selectively expressed in early stage follicles. The results indicate that global expression profiling of whole organ RNA provides sensitive first-line information about ovarian histogenesis for which no in vitro cell models are currently available.
authors.affiliation: Laboratory of Genetics, Gerentalogy Research Centre, National Institute on Aging, Suite 3000, 333 Cassell Drive, Baltimore, MD 21224, USA.
authors.forename: Luisa
authors.initials: L
authors.lastname: Herrera
authors.forename: Chris
authors.initials: C
authors.lastname: Ottolenghi
authors.forename: J Elias
authors.initials: JE
authors.lastname: Garcia-Ortiz
authors.forename: Massimo
authors.initials: M
authors.lastname: Pellegrini
authors.forename: Francesca
authors.initials: F
authors.lastname: Manini
authors.forename: Minoru S H
authors.initials: MS
authors.lastname: Ko
authors.forename: Ramaiah
authors.initials: R
authors.lastname: Nagaraja
authors.forename: Antonino
authors.initials: A
authors.lastname: Forabosco
authors.forename: David
authors.initials: D
authors.lastname: Schlessinger
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010053:Ovary / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D012313:RNA / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D012313:RNA