Production and characterization of monoclonal antibodies against human ceruloplasmin.
Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57 %. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.
Eum
Won Sik
WS
Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
Choi
Hee Soon
HS
Kim
Dae Won
DW
Jang
Sang Ho
SH
Choi
Soo Hyun
SH
Kim
So Young
SY
Park
Jinseu
J
Kang
Jung Hoon
JH
Cho
Sung-Woo
SW
Kwon
Oh-Shin
OS
Hwang
In Koo
IK
Yoo
Ki-Yeon
KY
Kang
Tae-Cheon
TC
Won
Moo Ho
MH
Choi
Soo Young
SY
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D001921:Brain / Q000276:immunology / Q000378:metabolism; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002570:Ceruloplasmin / Q000276:immunology* / Q000378:metabolism; D002645:Chickens; D003429:Cross Reactions; D004285:Dogs; D018604:Epitope Mapping; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D002570:Ceruloplasmin