title: Molecular epidemiology of adenovirus infection among pediatric population with diarrhea in Asia.
abstract: A total of 3,577 fecal specimens from infants and children with acute gastroenteritis in Japan, Korea and Vietnam during 1998 and 2001 were tested for adenovirus by the ELISA method. Of these, adenovirus was detected in 158 (4.4%). The detection rate of adenovirus was highest in Korea (8.7%, 20/231) followed by 5.0% (100/1,991) in Japan and 2.8% (38/1,355) in Vietnam. All adenoviruses were further serotyped by PCR-RFLP. The diversity of adenovirus serotypes including Ad2, 3, 5, 8, 31, 40 and 41 was demonstrated. Worth of note was a decrease in the rate of isolation of Ad40 (7.6%, 12/158) and a concomitant increase of Ad41 (63.9%, 101/158) to become the predominant serotype. Another interesting feature of the study was the presence of Ad3 (4.0% and 30%) and Ad8 (8.0% and 20%) in Japan and Korea, respectively, which is mainly associated with keratoconjunctivitis worldwide. Our result underscored the importance of adenovirus in association with acute gastroenteritis in Asian countries.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Japan.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.forename: Tuan Anh
authors.initials: TA
authors.lastname: Nguyen
authors.forename: Kyo Sun
authors.initials: KS
authors.lastname: Kim
authors.forename: Jeong Kee
authors.initials: JK
authors.lastname: Seo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Eiko
authors.initials: E
authors.lastname: Suzuki
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000235:genetics*; D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000821:virology; D001208:Asia / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007223:Infant; D012150:Polymorphism, Restriction Fragment Length; D012621:Seasons; D012703:Serotyping
chemical_list: D004279:DNA, Viral