title: Trichloroethylene (TCE) removal in a single pulse suspension bioreactor.
abstract: This work describes TCE biotic removal in a single-pulse bioreactor under aerobic conditions. Activated sludge from a wastewater-treatment plant was used for inoculation of the cultivator. The experiment focused on a more detailed verification of microbial composition of mixed heterotrophic culture during pulsed phenol dosage. Attention was given to suppressing eucaryotic organisms, particularly yeasts and fungi, by the addition of cycloheximide. The TCE-removal capacity of the heterotrophic culture, described by kinetic tests, was dependent on pulsed phenol injection and on cyclic addition of phenol and TCE. Maximum TCE degradation was determined in a batch test. It was found that the addition of cycloheximide (an antibiotic against propagation and growth of fungi and yeast) increased the TCE degradation activity of the mixed microbial suspension. A certain residual amount of TCE remained in some of the experiments.
authors.affiliation: Faculty of Technology, Department of Physics and Material Engineering, Tomas Bata University in Zlín, namesti T.G. Masaryka 275, 762 72 Zlín, Czech Republic. volcik@ft.utb.cz
authors.forename: Viktor
authors.initials: V
authors.lastname: Volcík
authors.forename: Jaromír
authors.initials: J
authors.lastname: Hoffmann
authors.forename: Jan
authors.initials: J
authors.lastname: Růzicka
authors.forename: Magda
authors.initials: M
authors.lastname: Sergejevová
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism; D001673:Biodegradation, Environmental; D019149:Bioreactors; D015169:Colony Count, Microbial; D003513:Cycloheximide / Q000378:metabolism; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D014241:Trichloroethylene / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D010636:Phenols; D012722:Sewage; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D003513:Cycloheximide; D010100:Oxygen