Color coding cancer cells with fluorescent proteins to visualize in vivo cellular interaction in metastatic colonies.
We report here in vivo visualization of cancer cell interaction using stable sublines of green fluorescent protein (GFP)- and red fluorescent protein (RFP)-expressing HT-1080 human fibrosarcoma cells. These color-coded fibrosarcoma cell lines show similar cell proliferation and lung metastasis potential. The color-coded cells were mixed at a ratio of 1:1 and were injected into the tail vein of severe combined immunodeficient (SCID) mice. The resulting experimental lung metastases were simultaneously imaged and their ratio was determined by color-pixel analysis. Fluorescence color coding of cancer cells enables visualization of the interaction of cancer cells and can be used to distinguish cancer cells of any genotype or phenotype.
Yamamoto
Norio
N
AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA 92111, USA.
Yang
Meng
M
Jiang
Ping
P
Xu
Mingxu
M
Yamauchi
Kensuke
K
Tsuchiya
Hiroyuki
H
Tomita
Katsuro
K
Moossa
A R
AR
Hoffman
Robert M
RM
D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D005354:Fibrosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D014161:Transduction, Genetic; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein