title: [A study on quality standard for Herba Siegesbeckidae].
abstract: OBJECTIVE
  To establish the qualitative and quantitative methods of Herba Siegesbeckiae.
  
  
  METHODS
  A TLC method was used for qualitative identification and a HPLC analysis was applied for quantitative determination of Herba Siegesbeckiae with kirenol as the reference substances.
  
  
  RESULTS
  Chloroform-methanol-formic acid (25:5:1) as a mobile phase of TLC, the spot of kirenol can be easily detected; Methanol extracts of Herba Siegebeckiae were separated on a Polaris C18 column with acetonitrile-water (25:75) as mobile phase and kirenol was separated well. The average content of kirenol in Herba Siegebeckiae was 0.14%. A good linear relationship between the peak areas and injected amounts of kirenol in the range of 0.19-14.9 microg and the average recovery was 100.0% (RSD = 2.4%).
  
  
  CONCLUSIONS
  The method can be used for qualitative identification and quantitation determination of Herba Siegesbeckiae.
authors.affiliation: Institute of Chinese Materia Medica of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Cheng
authors.forename: Gui-xin
authors.initials: GX
authors.lastname: Chou
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010598:Pharmacognosy / Q000592:standards; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal