title: Altered expression of type I insulin-like growth factor receptor in Crohn's disease.
abstract: The fibrotic and antiapoptotic effects of insulin-like growth factors (IGF) are mediated by type I IGF receptor (IGF-1R). IGFs could play a role in intestinal stricturing and in the maintenance of inflammation in Crohn's disease (CD). We aimed to describe IGF-1R expression in CD intestinal lesions, to compare it to other intestinal inflammatory diseases and to correlate it with fibrosis and apoptosis. IGF-1R expression and apoptosis (active caspase-3) were studied by immunohistochemistry. Surgical intestinal specimens [17 CD, nine controls, six diverticulitis and four ulcerative colitis (UC)] were used. IGF-1R was expressed transmurally mainly by inflammatory cells (IC) and smooth muscle cells, both in diseased intestine and controls. IGF-1R positive IC were increased in the mucosa and the submucosa of CD (P < 0.007), and in involved areas compared to uninvolved areas (P = 0.03). In UC, the number of IGF-1R positive IC was only increased in the mucosa, and was not different from controls in the submucosa. In diverticulitis, the number of IGF-1R positive IC did not differ from controls. In CD submucosa, IGF-1R expression in IC was inversely correlated with apoptosis in uninvolved areas (P = 0.01). Expression of IGF-1R in submucosal fibroblast-like cells, subserosal adipocytes and hypertrophic nervous plexi was specific for CD. We have shown a transmural altered expression of IGF-1R in CD. This may suggest a role for IGF-1R in the maintenance of chronic inflammation and stricture formation in CD.
authors.affiliation: Department of Gastroenterology, CHU of Liège, Liège, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: El Yafi
authors.forename: R
authors.initials: R
authors.lastname: Winkler
authors.forename: P
authors.initials: P
authors.lastname: Delvenne
authors.forename: N
authors.initials: N
authors.lastname: Boussif
authors.forename: J
authors.initials: J
authors.lastname: Belaiche
authors.forename: E
authors.initials: E
authors.lastname: Louis
mesh_terms: D017209:Apoptosis; D016022:Case-Control Studies; D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D003106:Colon / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D003424:Crohn Disease / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005355:Fibrosis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017526:Receptor, IGF Type 1 / Q000032:analysis* / Q000378:metabolism
chemical_list: D017526:Receptor, IGF Type 1; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases
references.citation: Am J Respir Crit Care Med. 2003 Jan 1;167(1):83-90
references.pmid: 12502480
references.citation: Immunology. 2002 Dec;107(4):461-71
references.pmid: 12460191
references.citation: Anat Rec A Discov Mol Cell Evol Biol. 2003 Jun;272(2):503-13
references.pmid: 12740944
references.citation: World J Gastroenterol. 2003 Jun;9(6):1287-91
references.pmid: 12800242
references.citation: Gastroenterology. 1988 Feb;94(2):257-65
references.pmid: 3335305
references.citation: Am J Respir Cell Mol Biol. 1991 May;4(5):432-9
references.pmid: 1850606
references.citation: Endocrinology. 1991 Oct;129(4):1769-78
references.pmid: 1655382
references.citation: J Immunol. 1992 Jan 1;148(1):63-71
references.pmid: 1345791
references.citation: Gastroenterology. 1992 Jun;102(6):1920-9
references.pmid: 1587410
references.citation: Br J Surg. 1992 Sep;79(9):955-8
references.pmid: 1422768
references.citation: Cell. 1993 Oct 8;75(1):59-72
references.pmid: 8402901
references.citation: Endocr Rev. 1995 Feb;16(1):3-34
references.pmid: 7758431
references.citation: J Biol Chem. 1996 May 10;271(19):11330-8
references.pmid: 8626686
references.citation: Clin Exp Immunol. 1996 May;104(2):280-5
references.pmid: 8625521
references.citation: Baillieres Clin Gastroenterol. 1996 Mar;10(1):83-96
references.pmid: 8732302
references.citation: Int Arch Occup Environ Health. 1997;69(3):157-64
references.pmid: 9049666
references.citation: Eur J Gastroenterol Hepatol. 1997 May;9(5):505-8
references.pmid: 9187885
references.citation: Gastroenterology. 1997 Sep;113(3):817-24
references.pmid: 9287973
references.citation: J Clin Invest. 1997 Sep 15;100(6):1425-39
references.pmid: 9294108
references.citation: Am J Physiol. 1997 Oct;273(4 Pt 1):G875-82
references.pmid: 9357830
references.citation: Gastroenterology. 1999 Mar;116(3):557-65
references.pmid: 10029614
references.citation: Oncogene. 1999 Mar 11;18(10):1827-36
references.pmid: 10086337
references.citation: Am J Physiol. 1999 Apr;276(4 Pt 1):G817-27
references.pmid: 10198323
references.citation: Lab Invest. 1999 Apr;79(4):395-405
references.pmid: 10211992
references.citation: Gastroenterology. 1999 Jul;117(1):73-81
references.pmid: 10381912
references.citation: Eur J Immunol. 2000 Apr;30(4):1010-8
references.pmid: 10760788
references.citation: Cancer Res. 2000 Apr 1;60(7):2007-17
references.pmid: 10766192
references.citation: Shock. 2000 May;13(5):361-6
references.pmid: 10807010
references.citation: Am J Physiol Gastrointest Liver Physiol. 2000 Dec;279(6):G1307-22
references.pmid: 11093955
references.citation: Virchows Arch. 2000 Dec;437(6):611-7
references.pmid: 11193472
references.citation: Inflamm Bowel Dis. 2001 Feb;7(1):16-26
references.pmid: 11233656
references.citation: Am J Physiol Gastrointest Liver Physiol. 2001 May;280(5):G1022-9
references.pmid: 11292612
references.citation: J Biol Chem. 2001 Mar 9;276(10):7187-94
references.pmid: 11120746
references.citation: Inflamm Bowel Dis. 2001 Aug;7(3):226-36
references.pmid: 11515849
references.citation: J Mol Cell Cardiol. 2001 Oct;33(10):1777-89
references.pmid: 11603921
references.citation: J Endocrinol Invest. 2001 Dec;24(11):856-64
references.pmid: 11817709
references.citation: Growth Horm IGF Res. 2002 Aug;12(4):193-7
references.pmid: 12175651
references.citation: Am J Physiol Gastrointest Liver Physiol. 2002 Oct;283(4):G875-85
references.pmid: 12223347
references.citation: Neuropeptides. 2002 Apr-Jun;36(2-3):209-20
references.pmid: 12359511
references.citation: Cell. 2003 Apr 18;113(2):147-58
references.pmid: 12705864