title: Effect of platelet-rich plasma with autogenous bone graft for maxillary sinus augmentation in a rabbit model.
abstract: OBJECTIVE
  To evaluate the effect of platelet-rich plasma (PRP) on autogenous bone graft remodeling during sinus augmentation in a rabbit model.
  
  
  METHODS
  Twelve New Zealand White rabbits were divided randomly into 3 groups based on their time of sacrifice (2, 4, and 8 weeks). All animals underwent a general anesthetic and harvesting of an autogenous bone graft from the right iliac crest with subsequent bilateral maxillary sinus augmentation. PRP was prepared via standard approved technique by acquiring 21 cc of autogenous blood and performing differential centrifugation to obtain PRP. One cc of PRP was produced that was mixed with bovine topical thrombin and calcium chloride. The left maxillary sinus received only autogenous bone, while the right maxillary sinus received a mixture of PRP mixed with autogenous bone, thus each animal acted as its own control. Equal volumes of bone were inserted in each maxillary sinus. Animals were sacrificed at 2, 4, and 8 weeks and all specimens were harvested for peripheral quantitative computed tomography (pQ-CT), static, and dynamic and histomorphometric analysis.
  
  
  RESULTS
  Student t tests were performed comparing bone density via pQ-CT analysis, histomorphometric parameters of total bone area, and bone apposition rate. PRP had no statistically significant effect on bone graft healing in maxillary sinus augmentation when compared using standard pQ-CT, static, and dynamic histologic criteria.
  
  
  CONCLUSIONS
  This study fails to find a direct stimulatory effect of PRP on healing of autogenous bone grafts using pQ-CT, static, and dynamic histomorphometric analyses.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Connecticut, Farmington, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Butterfield
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Bennett
authors.forename: Gloria
authors.initials: G
authors.lastname: Gronowicz
authors.forename: Douglas
authors.initials: D
authors.lastname: Adams
mesh_terms: D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D001804:Blood Transfusion, Autologous; D015519:Bone Density / Q000502:physiology; D016723:Bone Remodeling / Q000502:physiology; D016025:Bone Transplantation / Q000473:pathology; D002417:Cattle; D005456:Fluorescent Dyes; D008437:Maxilla / Q000473:pathology / Q000601:surgery*; D008443:Maxillary Sinus / Q000473:pathology / Q000601:surgery*; D023421:Models, Animal; D010012:Osteogenesis / Q000502:physiology; D010949:Plasma; D017713:Platelet Transfusion; D011817:Rabbits; D011897:Random Allocation; D013997:Time Factors; D014057:Tomography, X-Ray Computed; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000502:physiology
chemical_list: D005456:Fluorescent Dyes