title: Loss of heterozygosity by mitotic recombination in diploid strain of Aspergillus nidulans in response to castor oil plant detergent.
abstract: Somatic recombination in heterozygous diploid cells may be a promotional agent of neoplasms by inducing homozygosity of defective genes. Tumor suppressor genes may in this way be completely suppressed in recombinant cells. In this work, the genotoxic effects of detergent derived from the castor oil plant (Ricinus communis) in heterozygous diploid cells of Aspergillus nidulans are evaluated. Previous studies have evaluated the application of this substance in endodontic treatments as an irrigating solution. The recombinogenic potential of the compound has been studied through the production of homozygous cells for nutritional markers riboA1, pabaA124, biA1, methA17, and pyroA4. Detergent was diluted to 1:10, 1:20, and 1:40, and morphologic alterations, delay in conidiophore development, and mitotic recombination occurrence were reported for the three dilutions. Although past studies have demonstrated the antimicrobial action of the detergent under analysis, our results revealed its cytotoxic effects and recombinogenic potential.
authors.affiliation: Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, CEP 87020-900, Rio de Janeiro, RJ, Brazil.
authors.forename: S A
authors.initials: SA
authors.lastname: Souza-Júnior
authors.forename: E A L
authors.initials: EA
authors.lastname: Gonçalves
authors.forename: S A
authors.initials: SA
authors.lastname: Catanzaro-Guimarães
authors.forename: M A A
authors.initials: MA
authors.lastname: Castro-Prado
mesh_terms: D001233:Aspergillus nidulans / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D002368:Castor Oil / Q000633:toxicity*; D003434:Crossing Over, Genetic / Q000187:drug effects* / Q000235:genetics; D003902:Detergents / Q000633:toxicity*; D004171:Diploidy; D019656:Loss of Heterozygosity / Q000187:drug effects* / Q000235:genetics; D008938:Mitosis / Q000187:drug effects / Q000235:genetics; D009152:Mutagenicity Tests / Q000379:methods
chemical_list: D003902:Detergents; D002368:Castor Oil