title: Golgin tethers define subpopulations of COPI vesicles.
abstract: Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transport through tethering coat protein complex I (COPI) vesicles. The p115-golgin tether is the best studied, and here we characterize the golgin-84-CASP tether. The vesicles bound by this tether were strikingly different from those bound by the p115-golgin tether in that they lacked members of the p24 family of putative cargo receptors and contained enzymes instead of anterograde cargo. Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport to the endoplasmic reticulum, further implicating this tether in retrograde transport. These and other golgins may modulate the flow patterns within the Golgi stack.
authors.affiliation: Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520-8002, USA.
authors.forename: Jörg
authors.initials: J
authors.lastname: Malsam
authors.forename: Ayano
authors.initials: A
authors.lastname: Satoh
authors.forename: Laurence
authors.initials: L
authors.lastname: Pelletier
authors.forename: Graham
authors.initials: G
authors.lastname: Warren
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D001667:Binding, Competitive; D022181:COP-Coated Vesicles / Q000378:metabolism*; D002458:Cell Fractionation; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D006801:Humans; D047468:Immunoprecipitation; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008854:Microscopy, Electron; D021381:Protein Transport; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014157:Transcription Factors; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D001324:Autoantigens; C088424:Cux1 protein, rat; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; C028391:G protein, vesicular stomatitis virus; D000077614:Golgi Matrix Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014759:Viral Envelope Proteins; C496921:golgin-84, rat; C087574:macrogolgin