title: Natural killer T-cell populations in C57BL/6 and NK1.1 congenic BALB.NK mice-a novel thymic subset defined in BALB.NK mice.
abstract: Natural killer (NK) T lymphocytes are a subpopulation of T lymphocytes regarded as early regulators of immune responses. The majority of NKT cells are restricted by the CD1d molecule. NKT cells have mostly been studied in one single mouse strain, C57BL/6 (B6), because of the absence of NK1.1 in other common mouse strains, and the lack of other reliable surface markers for CD1d-restricted cells. To investigate NKT cell subsets in a mouse strain of a genetic background different from B6, we have back-crossed the NKT cell marker NK1.1 from the B6 mouse to the BALB/c mouse strain. We show that NKT cells in the congenic BALB.B6-NK1.1(b) mouse share many characteristics with their B6 counterparts, but seem to be deficient in the functional NKT cell subtype characterized by low interleukin-4 and high interferon-gamma production, and surface expression of CD49b but not CD69. Moreover, in the thymus but not the spleen of BALB.B6-NK1.1(b) mice we find a novel Valpha14-Jalpha18 invariant NKT cell subset which is devoid of a set of NK markers, suggesting that these cells represent a less differentiated NKT cell stage, and carries high levels of the T-cell receptor and uses a skewed T-cell receptor Vbeta-repertoire.
authors.affiliation: Section for Immunology, Lund University, Lund, Sweden. Martin.Stenstrom@immuno.lu.se
authors.forename: Martin
authors.initials: M
authors.lastname: Stenström
authors.forename: Markus
authors.initials: M
authors.lastname: Sköld
authors.forename: Asa
authors.initials: A
authors.lastname: Andersson
authors.forename: Susanna L
authors.initials: SL
authors.lastname: Cardell
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000032:analysis; D055610:Antigens, CD1d; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*
chemical_list: D018949:Antigens, CD1; D055610:Antigens, CD1d; D016207:Cytokines
references.citation: Annu Rev Immunol. 1999;17:297-329
references.pmid: 10358761
references.citation: J Immunol. 1999 Sep 15;163(6):3083-91
references.pmid: 10477573
references.citation: Eur J Immunol. 2000 Jan;30(1):236-44
references.pmid: 10602046
references.citation: Immunity. 2000 Feb;12(2):211-21
references.pmid: 10714687
references.citation: J Exp Med. 2000 Jun 5;191(11):1895-903
references.pmid: 10839805
references.citation: Scand J Immunol. 2000 Jul;52(1):71-9
references.pmid: 10886786
references.citation: J Exp Med. 2000 Sep 4;192(5):741-54
references.pmid: 10974039
references.citation: Semin Immunol. 2000 Dec;12(6):551-60
references.pmid: 11145861
references.citation: Int Immunol. 2001 Jul;13(7):887-96
references.pmid: 11431419
references.citation: J Immunol. 2001 Aug 1;167(3):1164-73
references.pmid: 11466330
references.citation: Curr Opin Immunol. 2002 Apr;14(2):165-71
references.pmid: 11869887
references.citation: J Exp Med. 2002 Mar 4;195(5):625-36
references.pmid: 11877485
references.citation: J Exp Med. 2002 Mar 4;195(5):637-41
references.pmid: 11877486
references.citation: J Exp Med. 2002 Apr 1;195(7):835-44
references.pmid: 11927628
references.citation: J Immunol. 2003 Jun 15;170(12):5815-9
references.pmid: 12794105
references.citation: Infect Immun. 2003 Oct;71(10):5447-55
references.pmid: 14500461
references.citation: Curr Opin Immunol. 2003 Dec;15(6):683-9
references.pmid: 14630203
references.citation: Immunology. 2003 Nov;110(3):313-21
references.pmid: 14632658
references.citation: Eur J Immunol. 2004 Jan;34(1):56-65
references.pmid: 14971030
references.citation: J Immunol. 2004 Sep 1;173(5):3112-8
references.pmid: 15322171
references.citation: J Immunol. 1991 Nov 1;147(9):3229-36
references.pmid: 1680927
references.citation: J Immunol. 1992 Sep 15;149(6):1957-63
references.pmid: 1517565
references.citation: J Exp Med. 1994 Apr 1;179(4):1285-95
references.pmid: 7908323
references.citation: Immunogenetics. 1995;41(2-3):148-51
references.pmid: 7806288
references.citation: Science. 1995 May 12;268(5212):863-5
references.pmid: 7538697
references.citation: J Exp Med. 1995 Oct 1;182(4):993-1004
references.pmid: 7561702
references.citation: J Exp Med. 1997 Apr 21;185(8):1395-401
references.pmid: 9126920
references.citation: Annu Rev Immunol. 1997;15:535-62
references.pmid: 9143699
references.citation: Science. 1997 Nov 28;278(5343):1626-9
references.pmid: 9374463
references.citation: J Immunol. 1998 Apr 1;160(7):3128-34
references.pmid: 9531267
references.citation: J Immunol. 1997 Dec 15;159(12):5931-5
references.pmid: 9550390
references.citation: J Immunol. 1999 Jan 1;162(1):161-7
references.pmid: 9886382
references.citation: Science. 2002 Apr 19;296(5567):553-5
references.pmid: 11968185
references.citation: Immunity. 2002 Jul;17(1):19-29
references.pmid: 12150888