[Protective effect of Armillaria mellea polysaccharide on mice bone marrow cell damage caused by cyclophosphamide].
OBJECTIVE
To observe the effect of Armillaria mellea polysaccharide on mice bone marrow cells damage caused by Cyclophosphamide.


METHODS
Kunming purebred mice were used and stochastic divided into 5 groups: normal control group, positive control group (rhG-CSF 20 microg x kg(-1) x d(-1)), damage group of Cyclophosphamide (150 mg x kg(-1) x d(-1)), the protective group with A. mellea polysaccharide, low dose (25.0 mg x kg(-1) x d(-1)) and high dose (50.0 mg x kg(-1) x d(-1)). Positive control was s.c. rhGCSF 6 d and i.p. Cyclophosphamide 3 d. A. mellea polysaccharide was i.p. 8 d. and Cyclophosphamide i.p. 3 d. WBC, RBC, PLT, BMNC were counted in peripheral blood and bone marrow cells. The myelogram were analyzed in bone marrow.


RESULTS
The WBC, RBC, PLT, BMNC of protective group and positive control group were higher than damage group (P < 0.01) significantly high dose group increaser than low dose group in protective group the numbed of Promyelocytic and lobulation nuclear of marrow.


CONCLUSIONS
A. mellea polysaccharide has preferably protective effect on damaging mice bone marrow cell caused by Cyclophosphamide.
Li
Yan-ping
YP
Guangdong Key Laboratory for Research Development of Natural Drug, Zhanjiang 524023, China. liyp0406@yahoo.com.cn
Wu
Ke-feng
KF
Liu
Yi
Y
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D010976:Platelet Count; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*
D011134:Polysaccharides; D020011:Protective Agents; D003520:Cyclophosphamide