Transcriptional expression of RPMS1 in nasopharyngeal carcinoma and its oncogenic potential.
The Epstein-Barr virus (EBV) BamHI A rightward transcripts (BARTs) were originally identified in C15 xenograft of nasopharyngeal carcinoma (NPC) and easily detected in a wide variety of EBV latent infection and EBV-associated tumors. It had been reported that p31 cosmid containing BARTs immortalized monkey epithelial cells, but which particular gene among BARTs family participates in the transformation procedure remains to be identified. RPMS1 is the only full-length cDNA confirmed so far and one of the most abundant spliced forms in BARTs family. To investigate the involvement of RPMS1 gene in NPC, we examined the expression of RPMS1 transcripts in NPC biopsies from Guangdong and its oncogenic potential. Our results revealed that RPMS1 mRNA preferentially expressed in primary NPC to non-carcinoma tissue of nasopharynx and peripheral blood lymphocytes (PBLs) of NPC patients. Furthermore, by introducing RPMS1 ORF into HEK293 cells, these transfectants enhanced the anchorage-independent growth and produced tumors in nude mice. These data imply that RPMS1 gene might play an important role in the development of NPC.
Li
Ang
A
Cancer Center, Sun Yat-sen University, Guangzho, China.
Zhang
Xiao-Shi
XS
Jiang
Ju-Hong
JH
Wang
Hong-He
HH
Liu
Xiao-Qiong
XQ
Pan
Zhi-Gang
ZG
Zeng
Yi-Xin
YX
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D002681:China / Q000453:epidemiology; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology
D009363:Neoplasm Proteins; D012333:RNA, Messenger; C423082:RPMS protein, Human herpesvirus 4; D014764:Viral Proteins