title: Immunohistochemical study of cyclosporin-induced gingival overgrowth in renal transplant recipients.
abstract: BACKGROUND
  Cyclosporin A (CsA) is an immunosuppressant widely used to treat transplant patients and various systemic diseases with immunological components. Gingival overgrowth (GO) is a common side effect of CsA administration; however, the pathogenesis of drug-induced GO is poorly understood. The aim of this study was to evaluate the expression of Ki-67, activation molecules (CD71, CD98), leukocytes activation antigens (CD45, CD45RA, CD50, CD11a, CD162, CD227, CD231), neurothelin (CD147), and novel endothelial cell antigens (B-F45, SCF87, B-D46, B-C44, VJ1/6) in gingival tissue in renal transplant recipients treated with CsA.
  
  
  METHODS
  Tissues from 15 renal transplant patients with significant GO and 10 systemically healthy control subjects with gingivitis were studied. Frozen-section biopsies were stained with monoclonal antibodies specific for the above-mentioned antigens using an indirect immunoperoxidase technique.
  
  
  RESULTS
  Comparison of the CsA-treated and control groups revealed no significant differences with respect to expression of Ki-67; CD50; activation molecules; neurothelin; or novel endothelial cell antigens B-D46, B-C44, and VJ1/6. However, expression patterns of CD45, CD45RA, CD11a, CD162, CD227, CD231, B-F45, and SCF87 were significantly different in CsA and control groups.
  
  
  CONCLUSIONS
  Leukocyte activation antigens play an important role in CsA-induced gingival overgrowth.
authors.affiliation: Hacettepe University, Faculty of Medicine, Department of Histology, Ankara, Turkey.
authors.forename: Omer Engin
authors.initials: OE
authors.lastname: Bulut
authors.forename: Lale Karakoç
authors.initials: LK
authors.lastname: Sökmensüer
authors.forename: Sule
authors.initials: S
authors.lastname: Bulut
authors.forename: Fügen
authors.initials: F
authors.lastname: Tasman
authors.forename: Sevda
authors.initials: S
authors.lastname: Müftüoğlu
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D016022:Case-Control Studies; D016572:Cyclosporine / Q000009:adverse effects*; D005260:Female; D005881:Gingiva / Q000276:immunology; D019214:Gingival Overgrowth / Q000139:chemically induced / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D019394:Ki-67 Antigen / Q000096:biosynthesis; D016030:Kidney Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D018709:Statistics, Nonparametric
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D007166:Immunosuppressive Agents; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; C055591:endothelial cell-monocyte antigens; D016572:Cyclosporine