title: Leptin concentrations in the abdominal subcutaneous adipose tissue of patients with anorexia nervosa assessed by in vivo microdialysis.
abstract: OBJECTIVE
  The adipocyte-derived hormone leptin is involved in energy metabolism and body weight regulation. Plasma leptin concentrations are significantly reduced in patients with anorexia nervosa (AN) and with severe malnutrition. Whether reduced plasma leptin is reflected by its decreased production by the adipose tissue is unknown.
  
  
  METHODS
  In the present study we measured leptin concentrations locally in the abdominal subcutaneous adipose tissue of 9 female AN patients and 11 healthy controls by in vivo microdialysis.
  
  
  RESULTS
  Adipose tissue free leptin levels were not different in patients with AN compared to controls (2.59+/-1.99 vs 2.36+/-0.25 ng/ml, P>0.05). Plasma leptin soluble receptor (sOb-R) levels were significantly higher in patients with AN than in healthy subjects (58.05+/-38.69 vs 12.79+/-5.08 U/ml, P<0.01). The area of adipocyte in AN was considerably smaller than in the controls (183+/-104.01 microm2 compared to 2145.8+/-1003.41).
  
  
  CONCLUSIONS
  We conclude that decreased plasma leptin levels in patients with AN are not directly related to dialysate leptin levels in the abdominal subcutaneous adipose tissue.
authors.affiliation: Institute of Endocrinology, Laboratory of Clinical and Experimental Neuroendocrinology, Narodni 8, Z116 94, Prague 1, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Dostálová
authors.forename: Vojtech
authors.initials: V
authors.lastname: Kopský
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Dusková
authors.forename: Hana
authors.initials: H
authors.lastname: Papezová
authors.forename: Karel
authors.initials: K
authors.lastname: Pacák
authors.forename: Jara
authors.initials: J
authors.lastname: Nedvídková
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000856:Anorexia Nervosa / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D017551:Microdialysis; D011956:Receptors, Cell Surface / Q000378:metabolism; D054411:Receptors, Leptin
chemical_list: C515448:LEPR protein, human; D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin