Plasma and semen ascorbic levels in spermatogenesis.
BACKGROUND
Conflicting reports on the mechanism of action of ascorbic acid level in male reproductive system exist and very little is known about the ascorbic acid status in Nigerian males with weak fertility.


METHODS
Ascorbate that accumulates preferentially in the testis, the lipid and lipoprotein levels were determined in the plasma of Nigerian males. Twenty-seven (27) male with inadequate spermatogenesis (36+/-1.0) years, with mean value of 15.6+/-6.90 million/cm3 sperm count and fourteen (14) controls (34+/-0.6) years, with mean value of 108.0+/-25.42 million/cm3 sperm count were selected for this study. The anthropometric indices were also determined.


RESULTS
There were highly significant decreases in sperm cell count, percentage motility and percentage vitality (p<0.001) in each case, while percentage morphologically abnormal sperm cells was significantly elevated (p<0.001) compared with the control values. There were significant decreases in the seminal and plasma ascorbic acid concentrations (p<0.001) in the males who had inadequate spermatogenesis compared with the control values. The plasma total cholesterol (TC) and body mass index (BMI) were not significantly different from the corresponding control values, but the plasma low density lipoprotein (LDLC) (p<0.001) and triglyceride (TG)(p<0.01) concentrations were significantly increased in all the patients. While the plasma high density lipoprotein cholesterol (HDLC)(p<0.001) was significantly decreased compared with the controls. The plasma lipid and lipoprotein levels did not demonstrate any definite pattern with the sperm characteristics.


CONCLUSIONS
The decreased semen ascorbate level may play a significant role in the reduced sperm characteristics in these patients.
Ebesunun
M O
MO
Departments of Chemical Pathology and Surgery, University College Hospital, Ibadan, Nigeria.
Solademi
B A
BA
Shittu
O B
OB
Anetor
J I
JI
Onuegbu
J A
JA
Olisekodiaka
J M
JM
Agbedana
E O
EO
Onyeaghala
A A
AA
D000328:Adult; D000975:Antioxidants; D001205:Ascorbic Acid / Q000097:blood*; D001206:Ascorbic Acid Deficiency / Q000150:complications*; D016022:Case-Control Studies; D006801:Humans; D007248:Infertility, Male / Q000209:etiology* / Q000503:physiopathology; D008297:Male; D009549:Nigeria; D012661:Semen / Q000187:drug effects*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects
D000975:Antioxidants; D001205:Ascorbic Acid