Sol-gel-based solid-phase microextraction and gas chromatography-mass spectrometry determination of dextromethorphan and dextrorphan in human plasma.
A novel solid-phase microextraction (SPME) method was developed for isolation of dextromethorphan (DM) and its main metabolite dextrorphan (DP) from human plasma followed by GC-MS determination. Three different polymers, poly(dimethylsiloxane) (PDMS), poly(ethylenepropyleneglycol) monobutyl ether (Ucon) and polyethylene glycol (PEG) were synthesized as coated fibers using sol-gel methodologies. DP was converted to its acetyl-derivative prior to extraction and subsequent determination. The porosity of coated fibers was examined by SEM technique. Effects of different parameters such as fiber coating type, extraction mode, agitation method, sample volume, extraction time, and desorption condition, were investigated and optimized. The method is rapid, simple, easy and inexpensive and offers high sensitivity and reproducibility. The limits of detection are 0.010 and 0.015 ng/ml for DM and DP, respectively. The precisions for both analytes are below 5% (n=5). The correlation coefficient was satisfactory (r(2)>0.99) for both DM and DP. Linear ranges were obtained from 0.03 ng/ml to 2 microg/ml for DM and from 0.05 ng/ml to 2 microg/ml for DP.
Bagheri
Habib
H
Department of Chemistry, Sharif University of Technology, Azadi Av., P.O. Box 11365-9516, Tehran, Iran. bagheri@sharif.edu
Es-haghi
Ali
A
Rouini
Mohammad-Reza
MR
D003915:Dextromethorphan / Q000097:blood* / Q000302:isolation & purification*; D003917:Dextrorphan / Q000097:blood* / Q000302:isolation & purification*; D004129:Dimethylpolysiloxanes / Q000138:chemical synthesis; D004987:Ethers / Q000138:chemical synthesis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000138:chemical synthesis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012828:Silicones / Q000138:chemical synthesis; D012996:Solutions / Q000138:chemical synthesis; D013696:Temperature
D004129:Dimethylpolysiloxanes; D004987:Ethers; D012828:Silicones; D012996:Solutions; C501377:poly(ethylenepropyleneglycol) monobutyl ether; D003917:Dextrorphan; D011092:Polyethylene Glycols; C013830:baysilon; D003915:Dextromethorphan