title: Characterisation of a cluster of TRIM-B30.2 genes in the chicken MHC B locus.
abstract: We have identified and characterised a cluster of six TRIM-B30.2 genes flanking the chicken BF/BL region of the B complex. The TRIM-B30.2 proteins are a subgroup of the TRIM protein family containing the tripartite motif (TRIM), consisting of a RING domain, a B-box and a coiled coil region, and a B30.2-like domain. In humans, a cluster of seven TRIM-B30.2 genes has been characterised within the MHC on Chromosome 6p21.33. Among the six chicken TRIM-B30.2 genes two are orthologous to those of the human MHC, and two (TRIM41 and TRIM7) are orthologous to human genes located on Chromosome 5. In humans, these last two genes are adjacent to GNB2L1, a guanine nucleotide-binding protein gene, the ortholog of the chicken c12.3 gene situated in the vicinity of the TRIM-B30.2 genes. This suggests that breakpoints specific to mammals have occurred and led to the remodelling of their MHC structure. In terms of structure, like their mammalian counterparts, each chicken gene consists of five coding exons; exon 1 encodes the RING domain and the B-box, exons 2, 3 and 4 form the coiled-coil region, and the last exon represents the B30.2-like domain. Phylogenetic analysis led us to assume that this extended BF/BL region may be similar to the human extended class I region, because it contains a cluster of BG genes sharing an Ig-V like domain with the BTN genes (Henry et al. 1997a) and six TRIM-B30.2 genes containing the B30.2-like domain, shared with the TRIM-B30.2 members and the BTN genes.
authors.affiliation: UPR 1983, CNRS, 7 rue Guy Môquet, 94801, Villejuif Cedex, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ruby
authors.forename: Bertrand
authors.initials: B
authors.lastname: Bed'Hom
authors.forename: Hakan
authors.initials: H
authors.lastname: Wittzell
authors.forename: Véronique
authors.initials: V
authors.lastname: Morin
authors.forename: Anne
authors.initials: A
authors.lastname: Oudin
authors.forename: Rima
authors.initials: R
authors.lastname: Zoorob
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000235:genetics*; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017433:Protein Structure, Secondary; D016415:Sequence Alignment
chemical_list: D015395:Histocompatibility Antigens Class I
references.citation: J Mol Evol. 1993 Dec;37(6):600-12
references.pmid: 8114113
references.citation: Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3958-62
references.pmid: 8632997
references.citation: Trends Biochem Sci. 1992 Sep;17(9):344-5
references.pmid: 1412709
references.citation: Nature. 2004 Feb 26;427(6977):848-53
references.pmid: 14985764
references.citation: Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8
references.pmid: 3162770
references.citation: Nat Genet. 2000 Jul;25(3):298-301
references.pmid: 10888877
references.citation: Scand J Immunol. 1981 Dec;14(6):607-16
references.pmid: 7043723
references.citation: J Hered. 1999 Jan-Feb;90(1):152-9
references.pmid: 9987924
references.citation: Mol Biol Evol. 1998 Dec;15(12):1696-705
references.pmid: 9866204
references.citation: Science. 1981 Sep 25;213(4515):1469-74
references.pmid: 6792704
references.citation: Immunol Rev. 1999 Feb;167:33-44
references.pmid: 10319249
references.citation: Proc Natl Acad Sci U S A. 1989 Jun;86(12):4594-8
references.pmid: 2499885
references.citation: Genomics. 2001 Feb 1;71(3):351-62
references.pmid: 11170752
references.citation: Immunol Rev. 1995 Feb;143:63-88
references.pmid: 7558083
references.citation: Immunogenetics. 2000 Apr;51(4-5):373-82
references.pmid: 10803852
references.citation: Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1941-4
references.pmid: 8700863
references.citation: Biochem Cell Biol. 1998;76(2-3):351-8
references.pmid: 9923704
references.citation: Immunogenetics. 1993;37(6):408-14
references.pmid: 8436415
references.citation: Nat Genet. 1997 Nov;17(3):285-91
references.pmid: 9354791
references.citation: Immunogenetics. 1997;46(5):383-95
references.pmid: 9271628
references.citation: Biochem Biophys Res Commun. 1997 Jun 9;235(1):162-5
references.pmid: 9196055
references.citation: Avian Dis. 1992 Apr-Jun;36(2):378-85
references.pmid: 1320867
references.citation: Tissue Antigens. 2003 Jan;61(1):63-71
references.pmid: 12622776
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Biochem Biophys Res Commun. 1998 Oct 20;251(2):515-9
references.pmid: 9792805
references.citation: Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1359-63
references.pmid: 1996336
references.citation: Prog Clin Biol Res. 1987;238:219-33
references.pmid: 3037561
references.citation: Cytogenet Cell Genet. 1996;75(1):7-9
references.pmid: 8995478
references.citation: Genetics. 1948 Nov;33(6):605
references.pmid: 18208304
references.citation: Proc Natl Acad Sci U S A. 1991 May 15;88(10):4377-81
references.pmid: 1903541
references.citation: Bioinformatics. 2001 Dec;17(12):1244-5
references.pmid: 11751241
references.citation: Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2550-4
references.pmid: 1826056
references.citation: J Virol. 2002 Apr;76(8):3810-8
references.pmid: 11907221
references.citation: EMBO J. 1988 Sep;7(9):2775-85
references.pmid: 3141149
references.citation: Immunogenetics. 1990;32(1):45-50
references.pmid: 2373526
references.citation: Poult Sci. 1982 Feb;61(2):205-11
references.pmid: 7088788
references.citation: J Virol. 2003 Jun;77(12 ):7101-5
references.pmid: 12768029
references.citation: Science. 1977 Jan 14;195(4274):193-5
references.pmid: 831269
references.citation: Oncogene. 1999 Feb 4;18(5):1209-17
references.pmid: 10022127
references.citation: Mol Cell Biol. 1988 Apr;8(4):1853-6
references.pmid: 3380101
references.citation: Biochem Biophys Res Commun. 2000 Sep 16;276(1):45-51
references.pmid: 11006080
references.citation: EMBO J. 2001 May 1;20(9):2140-51
references.pmid: 11331580
references.citation: Nature. 1999 Oct 28;401(6756):923-5
references.pmid: 10553909
references.citation: Nature. 2003 Oct 23;425(6960):805-11
references.pmid: 14574404