title: Detection and genotyping of human papillomavirus in cervical samples from Italian patients.
abstract: Human papillomaviruses (HPVs) are etiological agents of cervical cancer. In order to assess the epidemiological incidence and frequency of different HPV types, we applied a polymerase chain reaction (PCR)-direct sequencing approach based on the use of MY09/MY11 primers as compared to Hybrid Capture assay. Cervical samples were taken from 1,500 women, both with normal and abnormal cytological smears, and we found an incidence of 6.6% of HPV infection in Brescia. Overall, 97 samples tested HPV-positive, yielding 18 HPV types. The four most frequent HPV types were: HPV 16, -31, -6, and -58. This approach could be used in ordinary laboratory settings for quick and reliable typing of known and novel HPVs from clinical specimens and it could also be applied to anti-cancer vaccine development.
authors.affiliation: Institute of Microbiology and Virology, Spedali Civili-University of Brescia, 1 Piazzale Spedali Civili, 25123 Brescia, Italy. defrance@med.unibs.it
authors.forename: M A
authors.initials: MA
authors.lastname: De Francesco
authors.forename: F
authors.initials: F
authors.lastname: Gargiulo
authors.forename: C
authors.initials: C
authors.lastname: Schreiber
authors.forename: G
authors.initials: G
authors.lastname: Ciravolo
authors.forename: F
authors.initials: F
authors.lastname: Salinaro
authors.forename: N
authors.initials: N
authors.lastname: Manca
mesh_terms: D000293:Adolescent; D000328:Adult; D002278:Carcinoma in Situ / Q000821:virology; D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007558:Italy; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000821:virology*