title: Identification and characterization of undifferentiated mast cells in mouse bone marrow.
abstract: Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34(+), CD13(+), and c-kit(+), and does not express FcepsilonRI. However, by polymerase chain reaction (PCR) the cell contains message for the alpha and beta subunits of FcepsilonRI, mast cell-specific proteases, and carboxypeptidase A. Morphologically, this cell has a large nucleus, little cytoplasm, few cytoplasmic organelles, and no cytoplasmic granules. In vitro, in the presence of interleukin-3 (IL-3) and stem cell factor (SCF) these cells differentiate only into a granulated mast cell that now expresses CD13, c-kit, mast cell-specific gangliosides, FcepsilonRI, and binds immunoglobulin E (IgE). When injected into lethally irradiated mice, these cells are able to reconstitute the mast cell population in the spleen.
authors.affiliation: Faculdade de Medicina de Ribeirão Preto, Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
authors.forename: Maria Célia
authors.initials: MC
authors.lastname: Jamur
authors.forename: Ana Cristina G
authors.initials: AC
authors.lastname: Grodzki
authors.forename: Elsa H
authors.initials: EH
authors.lastname: Berenstein
authors.forename: Majed M
authors.initials: MM
authors.lastname: Hamawy
authors.forename: Reuben P
authors.initials: RP
authors.lastname: Siraganian
authors.forename: Constance
authors.initials: C
authors.lastname: Oliver
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D018826:CD13 Antigens; D002454:Cell Differentiation; D005260:Female; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D008297:Male; D008407:Mast Cells / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D015388:Organelles / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D017455:Receptors, IgE / Q000235:genetics
chemical_list: D018952:Antigens, CD34; D012333:RNA, Messenger; D017455:Receptors, IgE; D018826:CD13 Antigens