title: Haemopoietic mechanisms in murine allergic upper and lower airway inflammation.
abstract: Eosinophil recruitment to the airways, including involvement of haemopoietic eosinophil-basophil progenitors (Eo/B-CFU), is primarily regulated by interleukin-5 (IL-5) and eotaxin. In this study, we investigated the haemopoietic mechanisms in upper and lower airway eosinophilic inflammation. Ovalbumin (OVA) sensitized and challenged BALB/c mice were used to establish isolated upper (UAC), isolated lower (LAC), or combined upper and lower airway (ULAC) inflammation. Airway, blood and bone marrow responses were evaluated in each model. Numbers of airway eosinophils and CD4(+) cells were increased significantly in the nasal mucosa in UAC and ULAC mice, and in the lung tissue in LAC and ULAC groups. Levels of IL-5 and eotaxin were increased significantly in the nasal lavage fluid (NL) in UAC and ULAC mice, and in the bronchoalveolar lavage fluid (BAL) in LAC and ULAC groups. The proportion of IL-5-responsive bone marrow Eo/B-CFU was significantly higher than the control in all treatment groups, but peaked much earlier in the ULAC group. Kinetic studies revealed that IL-5 and eotaxin in NL, BAL and serum peaked between 2 and 12 hr after OVA challenge in ULAC mice, and at 24 hr in UAC mice, related to the timing of maximal progenitor responses. These data support the concept that the systemic mechanisms linking rhinitis to asthma depend on the location and extent of airway allergen exposure.
authors.affiliation: Department of Medicine, Division of Clinical Immunology and Allergy, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Hiroko
authors.initials: H
authors.lastname: Saito
authors.forename: Lynn
authors.initials: L
authors.lastname: Crawford
authors.forename: Mark D
authors.initials: MD
authors.lastname: Inman
authors.forename: Michael M
authors.initials: MM
authors.lastname: Cyr
authors.forename: Judah A
authors.initials: JA
authors.lastname: Denburg
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001249:Asthma / Q000276:immunology* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000032:analysis; D004195:Disease Models, Animal; D004804:Eosinophils / Q000473:pathology; D005260:Female; D006410:Hematopoiesis / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D018928:Immunity, Mucosal; D015848:Interleukin-5 / Q000032:analysis; D007958:Leukocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D017817:Nasal Lavage Fluid / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D012220:Rhinitis / Q000276:immunology* / Q000473:pathology
chemical_list: D018952:Antigens, CD34; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D019742:Chemokines, CC; D015848:Interleukin-5; D010047:Ovalbumin
references.citation: J Allergy Clin Immunol. 2000 Nov;106(5 Suppl):S235-41
references.pmid: 11080737
references.citation: J Allergy Clin Immunol. 1999 Oct;104(4 Pt 1):786-90
references.pmid: 10518822
references.citation: Clin Exp Allergy. 2001 May;31(5):782-90
references.pmid: 11422139
references.citation: Am J Respir Crit Care Med. 2001 Sep 1;164(5):858-65
references.pmid: 11549546
references.citation: Immunology. 2001 Oct;104(2):226-34
references.pmid: 11683963
references.citation: J Allergy Clin Immunol. 2001 Nov;108(5 Suppl):S147-334
references.pmid: 11707753
references.citation: Am J Respir Crit Care Med. 2002 Jul 15;166(2):146-53
references.pmid: 12119225
references.citation: Am J Respir Crit Care Med. 2002 Sep 15;166(6):883-9
references.pmid: 12231502
references.citation: J Allergy Clin Immunol. 2003 Jan;111(1):79-86
references.pmid: 12532100
references.citation: Clin Exp Allergy. 2003 May;33(5):579-87
references.pmid: 12752585
references.citation: Immunology. 2003 Aug;109(4):536-46
references.pmid: 12871220
references.citation: Am J Respir Crit Care Med. 2004 Mar 1;169(5):565-72
references.pmid: 14656753
references.citation: J Allergy Clin Immunol. 1985 Sep;76(3):466-72
references.pmid: 4031318
references.citation: J Allergy Clin Immunol. 1986 Aug;78(2):365-71
references.pmid: 3734290
references.citation: J Allergy Clin Immunol. 1988 May;81(5 Pt 1):782-90
references.pmid: 3286718
references.citation: Am Rev Respir Dis. 1989 Mar;139(3):806-17
references.pmid: 2923380
references.citation: Int J Cell Cloning. 1990 Jan;8 Suppl 1:147-53; discussion 153-4
references.pmid: 2182734
references.citation: Am Rev Respir Dis. 1991 Feb;143(2):331-5
references.pmid: 1990948
references.citation: J Allergy Clin Immunol. 1992 Feb;89(2):611-8
references.pmid: 1740589
references.citation: Br Med Bull. 1992 Jan;48(1):72-84
references.pmid: 1352170
references.citation: J Allergy Clin Immunol. 1994 Apr;93(4):725-34
references.pmid: 8163782
references.citation: J Allergy Clin Immunol. 1994 Dec;94(6 Pt 2):1135-41
references.pmid: 7528232
references.citation: Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12290-4
references.pmid: 8618887
references.citation: J Exp Med. 1996 Oct 1;184(4):1461-9
references.pmid: 8879217
references.citation: J Appl Physiol (1985). 1996 Oct;81(4):1739-43
references.pmid: 8904594
references.citation: Am J Respir Cell Mol Biol. 1996 Nov;15(5):645-55
references.pmid: 8918371
references.citation: Am J Respir Cell Mol Biol. 1997 May;16(5):510-20
references.pmid: 9160833
references.citation: Am J Respir Cell Mol Biol. 1997 Oct;17(4):404-13
references.pmid: 9376115
references.citation: J Allergy Clin Immunol. 1997 Nov;100(5):632-41
references.pmid: 9389293
references.citation: J Clin Invest. 1997 Nov 15;100(10):2466-75
references.pmid: 9366561
references.citation: Am J Respir Crit Care Med. 1998 Jan;157(1):99-105
references.pmid: 9445285
references.citation: Blood. 1998 Apr 1;91(7):2240-8
references.pmid: 9516121
references.citation: Proc Natl Acad Sci U S A. 1998 May 26;95(11):6273-8
references.pmid: 9600955
references.citation: J Allergy Clin Immunol. 1998 Jul;102(1):65-74
references.pmid: 9679849
references.citation: Am J Respir Crit Care Med. 1998 Sep;158(3):951-7
references.pmid: 9731030
references.citation: J Clin Invest. 1998 Sep 15;102(6):1132-41
references.pmid: 9739047
references.citation: Am J Respir Cell Mol Biol. 1999 Oct;21(4):473-9
references.pmid: 10502557
references.citation: J Allergy Clin Immunol. 2001 Mar;107(3):469-76
references.pmid: 11240947