title: [The study of the pro-nucleating activity of bacteria identified in cholesterol gallstones in model bile systems].
abstract: OBJECTIVE
  To explore the relationship of bacteria identified in cholesterol gallstones and gallstone formation.
  
  
  METHODS
  Observe the bacteria activity in model bile and the influence of bacteria on the cholesterol nucleation time (NT).
  
  
  RESULTS
  (1) Model bile were suitable for the growth of E. coli, Pseudomonas aeruginosa, staphylococcus aureus, enterococcus faecalis, clostridium difficile and Clostridium. Propionibacterium acne grew weakly and the growth of Bacteroides fragilis was restrained in model bile. (2) Only pseudomonas aeruginosa and enTerococcus faecalis could ly shorten the cholesterol nucleation time. (3) With pseudomonas aeruginosa or enTerococcus faecalis added in model bile, the formation of cholesterol crystals presented a progressive course of evolution.
  
  
  CONCLUSIONS
  Pseudomonas aeruginosa and enterococcus faecalis, not propionibacterium acne, have pro-nucleating ability in model bile.
authors.affiliation: Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Lei-ming
authors.initials: LM
authors.lastname: Zhu
authors.forename: Duan
authors.initials: D
authors.lastname: Cai
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lü
authors.forename: Wei-huan
authors.initials: WH
authors.lastname: Chen
authors.forename: Wen-feng
authors.initials: WF
authors.lastname: Wang
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Zhang
mesh_terms: D001646:Bile / Q000378:metabolism / Q000382:microbiology*; D002769:Cholelithiasis / Q000382:microbiology*; D002784:Cholesterol / Q000378:metabolism*; D003460:Crystallization; D013293:Enterococcus faecalis / Q000254:growth & development; D008954:Models, Biological; D011425:Propionibacterium acnes / Q000254:growth & development; D011550:Pseudomonas aeruginosa / Q000254:growth & development
chemical_list: D002784:Cholesterol