title: How many functional transport pathways does Plasmodium falciparum induce in the membrane of its host erythrocyte?
abstract: Intraerythrocytic malaria parasites induce considerable change in the permeability of the membrane of their host cell. Using classical techniques of radiolabel uptake and iso-osmotic lysis, the permeability characteristics of the host-cell membrane have been determined. In a recent analysis of these results, we concluded that there are at least two types of channel that conform to the data: a low copy number (four channels per cell) type that mediates the transport of cations, anions and most other osmolytes that were tested, and a high copy number (300-400 channels per cell) type that is an anion channel that could also mediate the translocation of purine nucleosides. Patch-clamping experiments using cells infected with Plasmodium falciparum reveal 200-1000 anion channels of more than one type that are of host-cell endogenous provenance. Recent reports show that parasites can grow normally in erythrocytes that lack these endogenous agencies and in which the anion channels are not expressed, although their osmolyte permeability is present. We suggest that only the latter type of channel is important for normal development of the parasite.
authors.affiliation: Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. hagai@vms.huji.ac.il
authors.forename: Hagai
authors.initials: H
authors.lastname: Ginsburg
authors.forename: Wilfred D
authors.initials: WD
authors.lastname: Stein
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002463:Cell Membrane Permeability; D004594:Electrophysiology; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000469:parasitology*; D006790:Host-Parasite Interactions; D007473:Ion Channels / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000502:physiology*
chemical_list: D007473:Ion Channels