Enhancement of photorespiration in immobilized Chlamydomonas reinhardtii cells.
Immobilization of Chlamydomonas reinhardtii in alginate increases its photorespiration rate. In the immobilized cells, the photorespiratory enzyme, phosphoglycolate phosphatase, was 75% higher than in freely suspended cells. Thus, the immobilized cells produced glycolate at twice the rate than in freely suspended cells when treated with aminooxyacetate (a transaminase inhibitor). With immobilized cells in a batch reactor, 270 micromol glycolate mg(-1) Chl was produced after 12 h.
Garbayo
I
I
Departamento de Química y CC.MM (Area de Bioquímica), Facultad de Ciencias Experimentales, Campus El Carmen, Universidad de Huelva, 21017, Huelva, Spain. garbayo@uhu.es
Forján
E
E
Salguero
A
A
Cuaresma
M
M
Vega
J M
JM
Vílchez
C
C
D000818:Animals; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D019069:Cell Respiration / Q000502:physiology* / Q000528:radiation effects; D018914:Cells, Immobilized / Q000502:physiology; D016825:Chlamydomonas reinhardtii / Q000502:physiology*; D006016:Glycolates / Q000378:metabolism*; D008027:Light; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis* / Q000528:radiation effects; D010788:Photosynthesis / Q000502:physiology*
D006016:Glycolates; C031149:glycolic acid; C022408:phosphoglycolate phosphatase; D010744:Phosphoric Monoester Hydrolases