Expression of human antibodies in eukaryotic micro-algae.
Protein based therapeutics have enjoyed great success over the past decade. Unfortunately, with this clinical success comes a heavy price tag, owing to the inherently high costs of capitalization and production using mammalian cell fermentation. To address this problem, we have begun developing a system for the expression of recombinant proteins in the unicellular eukaryotic green algae, Chlamydomonas reinhardtii, leading to the production of human IgA single chain antibodies. The expression of human monoclonal antibodies in C. reinhardtii offers an attractive alternative to traditional mammalian based expression systems for several reasons, including an ability to rapidly obtain stable plastid and nuclear transformants, coupled with inherently low costs of capitalization and production.
Mayfield
Stephen P
SP
Department of Cell Biology, Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA. mayfield@scripps.edu
Franklin
Scott E
SE
D000906:Antibodies / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D056890:Eukaryota / Q000378:metabolism*; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis
D000906:Antibodies; D011994:Recombinant Proteins