title: Mutation frequencies and AID activation state in B-cell lymphomas from Ung-deficient mice.
abstract: B-cell lymphomas arising in lymph nodes and spleen of aging mice deficient in the Ung DNA glycosylase were recovered, dispersed, grown in short-term culture, and CD19-positive B-cells retrieved and analysed. Several tumors as well as controls only expressed detectable amounts of the Aid deaminase after mitogenic stimulation, as estimated by real-time PCR of transcripts. However, one unusually large lymph node tumor expressed a high level of Aid constitutively. This particular tumor also showed a substantially increased mutation frequency in the Aid gene itself as well as in the bcl-6 and c-myc genes, but not in the p53 gene, consistent with aberrant somatic hypermutation. Other B-cell lymphomas from Ung(-/-) mice exhibited a modest increase in mutation frequency.
authors.affiliation: Clare Hall Laboratories, CR-UK London Research Institute, South Mimms, Hertfordshire EN6 3LD, UK.
authors.forename: Hilde
authors.initials: H
authors.lastname: Nilsen
authors.forename: Qian
authors.initials: Q
authors.lastname: An
authors.forename: Tomas
authors.initials: T
authors.lastname: Lindahl
mesh_terms: D000818:Animals; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: D012333:RNA, Messenger; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase