title: Amsacta moorei Entomopoxvirus inhibitor of apoptosis suppresses cell death by binding Grim and Hid.
abstract: Inhibitor of apoptosis (iap) genes have been identified in the genomes of two independent families of insect viruses, the Baculoviridae and the Entomopoxvirinae. In this report, we examined the functional attributes of the Amsacta moorei entomopoxvirus-encoded IAP protein (AMV-IAP). The binding specificity of the individual baculoviral IAP repeat (BIR) domains of AMV-IAP was investigated by using a random-peptide, phage display library, and sequences similar to the amino termini of proapoptotic Drosophila proteins in the Reaper/Hid/Grim family were identified. Furthermore, the BIR domains of AMV-IAP protein were demonstrated to bind the mammalian IAP inhibitor Smac through the AVPI tetrapeptide sequence, suggesting that the peptide binding pocket and groove found in the insect and mammalian IAPs is conserved in this viral protein. Interaction analysis implicated BIR1 as the high-affinity site for Grim, while BIR2 interacted more strongly with Hid. Both Grim and Hid were demonstrated to interact with AMV-IAP in vivo, and Grim- or Hid-induced cell death was suppressed when AMV-IAP was coexpressed.
authors.affiliation: Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Box 100266, Gainesville, FL 32610-0266, USA.
authors.forename: Qianjun
authors.initials: Q
authors.lastname: Li
authors.forename: Peter
authors.initials: P
authors.lastname: Liston
authors.forename: Natasha
authors.initials: N
authors.lastname: Schokman
authors.forename: Jenny Mei
authors.initials: JM
authors.lastname: Ho
authors.forename: Richard W
authors.initials: RW
authors.lastname: Moyer
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D017996:Entomopoxvirinae / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D047908:Intracellular Signaling Peptides and Proteins; D024101:Mitochondrial Proteins / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017385:Sequence Homology; D014162:Transfection; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; C412403:DIABLO protein, human; D029721:Drosophila Proteins; C108880:HID protein, Drosophila; D051034:Inhibitor of Apoptosis Proteins; D047908:Intracellular Signaling Peptides and Proteins; D024101:Mitochondrial Proteins; D009479:Neuropeptides; D019151:Peptide Library; D011506:Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C108881:grim protein, Drosophila
references.citation: J Biol Chem. 2002 Sep 27;277(39):36611-6
references.pmid: 12121969
references.citation: EMBO J. 2002 Oct 1;21(19):5130-40
references.pmid: 12356729
references.citation: J Biol Chem. 2002 Sep 20;277(38):35097-104
references.pmid: 12122017
references.citation: FEBS Lett. 2004 Jan 30;558(1-3):57-62
references.pmid: 14759516
references.citation: Methods Enzymol. 2000;327:578-93
references.pmid: 11045010
references.citation: EMBO J. 2002 Oct 1;21(19):5118-29
references.pmid: 12356728
references.citation: Nat Immunol. 2002 Nov;3(11):1013-8
references.pmid: 12407409
references.citation: J Biol Chem. 2003 Feb 28;278(9):7494-9
references.pmid: 12511567
references.citation: Nat Cell Biol. 2003 May;5(5):467-73
references.pmid: 12692559
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8662-7
references.pmid: 11447297
references.citation: Mol Pharmacol. 2003 Aug;64(2):334-45
references.pmid: 12869638
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2885-90
references.pmid: 10077606
references.citation: J Virol. 2001 Mar;75(6):2535-43
references.pmid: 11222676
references.citation: EMBO J. 1998 Dec 15;17(24):7199-208
references.pmid: 9857177
references.citation: J Biol Chem. 2002 Dec 13;277(50):48677-84
references.pmid: 12324475
references.citation: Curr Opin Cell Biol. 2003 Dec;15(6):717-24
references.pmid: 14644196
references.citation: EMBO J. 2003 Dec 15;22(24):6642-52
references.pmid: 14657035
references.citation: Cell. 2001 Mar 9;104(5):769-80
references.pmid: 11257230
references.citation: Science. 1997 Jan 24;275(5299):536-40
references.pmid: 8999799
references.citation: J Virol. 2005 Feb;79(4):2335-45
references.pmid: 15681434
references.citation: J Biol Chem. 2003 Dec 5;278(49):49517-22
references.pmid: 14512414
references.citation: Biochemistry. 2002 Nov 19;41(46):13663-71
references.pmid: 12427028
references.citation: Mol Cell. 2003 Feb;11(2):519-27
references.pmid: 12620238
references.citation: Virology. 2000 Aug 15;274(1):120-39
references.pmid: 10936094
references.citation: EMBO J. 2000 Feb 15;19(4):598-611
references.pmid: 10675329
references.citation: Biochem J. 2003 Aug 1;373(Pt 3):965-71
references.pmid: 12747801
references.citation: Cell. 2001 Mar 9;104(5):791-800
references.pmid: 11257232
references.citation: J Virol. 1999 Jan;73(1):533-52
references.pmid: 9847359
references.citation: Nat Cell Biol. 2002 Jun;4(6):445-50
references.pmid: 12021771
references.citation: EMBO J. 1997 May 15;16(10):2805-13
references.pmid: 9184225
references.citation: Science. 2000 May 5;288(5467):874-7
references.pmid: 10797013
references.citation: Cell Death Differ. 2001 Feb;8(2):137-43
references.pmid: 11313715
references.citation: Infect Genet Evol. 2002 Oct;2(1):3-10
references.pmid: 12797996
references.citation: J Virol. 1994 Apr;68(4):2521-8
references.pmid: 8139034
references.citation: J Virol. 1993 Apr;67(4):2168-74
references.pmid: 8445726
references.citation: Annu Rev Immunol. 2003;21:377-423
references.pmid: 12543935
references.citation: Immunol Cell Biol. 1999 Jun;77(3):236-41
references.pmid: 10361255
references.citation: Biochemistry. 2003 Jul 15;42(27):8223-31
references.pmid: 12846571
references.citation: Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4936-41
references.pmid: 10220397
references.citation: Oncogene. 2003 Nov 24;22(53):8568-80
references.pmid: 14634619
references.citation: FEBS Lett. 1998 Nov 27;440(1-2):243-8
references.pmid: 9862464
references.citation: Biochem Biophys Res Commun. 2003 May 9;304(3):499-504
references.pmid: 12729584
references.citation: Trends Biochem Sci. 2002 Feb;27(2):94-101
references.pmid: 11852247