Genes differentially expressed in human lung fibroblast cells transformed by glycidyl methacrylate.
OBJECTIVE
To define the differences in gene expression patterns between glycidyl methacrylate (GMA)-transformed human lung fibroblast cells (2BS cells) and controls.


METHODS
The mRNA differential display polymerase chain reaction (DD-PCR) technique was used. cDNAs were synthesized by reverse transcription and amplified by PCR using 30 primer combinations. After being screened by dot blot analysis, differentially expressed cDNAs were cloned, sequenced and confirmed by Northern blot analysis.


RESULTS
Eighteen differentially expressed cDNAs were cloned and sequenced, of which 17 were highly homologous to known genes (homology = 89%-100%) and one was an unknown gene. Northern blot analysis confirmed that eight genes encoding human zinc finger protein 217 (ZNF217), mixed-lineage kinase 3 (MLK-3), ribosomal protein (RP) L15, RPL41, RPS 16, TBX3, stanniocalcin 2 (STC2) and mouse ubiquitin conjugating enzyme (UBC), respectively, were up-regulated, and three genes including human transforming growth factor beta inducible gene (Betaig-h3), alpha-1,2-mannosidase 1A2 (MAN 1A2) gene and an unknown gene were down-regulated in the GMA-transformed cells.


CONCLUSIONS
Analysis of the potential function of these genes suggest that they may be possibly linked to a variety of cellular processes such as transcription, signal transduction, protein synthesis and growth, and that their differential expression could contribute to the GMA-induced neoplastic transformation.
Yin
Xue-Jun
XJ
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China. xyin@hsc.wvu.edu
Xu
Jian-Ning
JN
Zou
Chang-Qi
CQ
He
Feng-Sheng
FS
Fang
Fu-De
FD
D000395:Air Pollutants, Occupational / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D002461:Cell Line, Transformed; D004852:Epoxy Compounds / Q000633:toxicity*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology*; D008297:Male; D008361:Mannosidases / Q000187:drug effects / Q000378:metabolism; D008689:Methacrylates / Q000633:toxicity*; D048052:Mitogen-Activated Protein Kinase 3 / Q000187:drug effects / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012269:Ribosomal Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism; D016335:Zinc Fingers / Q000187:drug effects / Q000502:physiology
D000395:Air Pollutants, Occupational; D004852:Epoxy Compounds; D006023:Glycoproteins; D008689:Methacrylates; D012269:Ribosomal Proteins; D016212:Transforming Growth Factor beta; D014452:Ubiquitins; C051795:teleocalcin; D048052:Mitogen-Activated Protein Kinase 3; D008361:Mannosidases; C007870:glycidyl methacrylate