Messenger RNA fingerprinting analysis using arbitrarily primed PCR (RAP) of genes expressed during rat C6 glioma cell differentiation.
To identify differentially expressed genes involved in rat C6 glioma cell differentiation induced by cyclic AMP, we adopted mRNA fingerprinting using arbitrarily primed polymerase chain reaction (PCR) (RAP). Four complementary DNA (cDNA) fragments differentially expressed during differentiation were isolated, and they appeared to contain coding regions of corresponding mRNAs. RAP can be used to efficiently identify cDNA fragments by comparing nucleotide and deduced amino acid sequences with those in databases, and is thus a useful method to search for and identify important genes involved in complex cellular processes such as glioma cell differentiation.
Sakai
H
H
Department of Neurosurgery, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan. nohgeka@cc.gifu-u.ac.jp
Nakashima
S
S
Nakatani
K
K
Yoshimura
S
S
Nishimura
Y
Y
Sakai
N
N
Nozawa
Y
Y
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D048430:Cell Shape; D016543:Central Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology*; D016172:DNA Fingerprinting / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013806:Theophylline / Q000494:pharmacology; D015854:Up-Regulation
D018076:DNA, Complementary; D012333:RNA, Messenger; D003994:Bucladesine; D013806:Theophylline