[Phylogenetic relationship between tufted deer(Elaphodus cephalophus) and Muntiacus deer is revealed by the exon and intron of K+ channel gene].
In this study, partial fragments of potassium ion channel gene were amplified using the genomic DNA of muntjak, reevesi, crinifrons, and Elaphodus cephalophus. The PCR products were ligated to the plasmid of pMD18-T Vector by the method of direct T-A cloning. The positive clones were identified by colony PCR. The sequences of the recombinant clones were determined using M13-47/RV-M universal primers and aligned by the software CLUSTALW. The nucleotide divergences of exon were 0.90%-1.44% among three species of Muntiacus, 0.90%-1.26% between E. cephalophus and each of Muntiacus deer. In the nucleotide of intron there is 0%-1.22% difference among these muntjac deers, and the divergene reached about 1.83% between E. cephalophus and the three species of Muntiacus. Using the software of MEGA to analyse molecular phylogeny, Phylogenetic trees were constructed with neighbor-joining method and maximum parsimony method. The result showed Muntiacus, crinifrons is most closely related to muntjak, with reevesi as their sister species. E. cephalophus is in the other genus.
Dai
Jun-Yong
JY
College of Life Science, Nanjing Normal University, Nanjing 210097, China. daijunyong@njnu.edu.cn
Cao
Xiang-Rong
XR
Shi
Lei
L
Zhang
Xi-Ran
XR
Xu
Chun-Mao
CM
Hu
Jun
J
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D003670:Deer / Q000145:classification* / Q000235:genetics; D005091:Exons; D007438:Introns; D018668:Muntjacs / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D015221:Potassium Channels / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015221:Potassium Channels; D004247:DNA