title: The expression of the SWI/SNF ATPase subunits BRG1 and BRM in normal human tissues.
abstract: SWI/SNF is a chromatin-remodeling complex important in gene regulation, cytokine responses, tumorigenesis, differentiation, and development. As a multitude of signaling pathways require SWI/SNF, loss of SWI/SNF function is expected to have an impact on cellular phenotypes. The SWI/SNF ATPase subunits, BRG1 and BRM, have been shown to be lost in a subset of human cancer cell lines and human primary cancers and may represent tumor suppressor proteins. To better understand the biology of these proteins, the authors examined the expression pattern of BRG1 and BRM in a variety of normal tissues. BRG1 expression was predominantly seen in cell types that constantly undergo proliferation or self-renewal; in contrast, BRM was preferentially expressed in brain, liver, fibromuscular stroma, and endothelial cell types, cell types not constantly engaged in proliferation or self-renewal. This differential expression suggests that these proteins serve distinct functions in human tissues.
authors.affiliation: Division of Hematology/Oncology, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: David N
authors.initials: DN
authors.lastname: Reisman
authors.forename: Janiece
authors.initials: J
authors.lastname: Sciarrotta
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Bouldin
authors.forename: Bernard E
authors.initials: BE
authors.lastname: Weissman
authors.forename: William K
authors.initials: WK
authors.lastname: Funkhouser
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D004265:DNA Helicases; D029721:Drosophila Proteins / Q000378:metabolism*; D004475:Ectoderm / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008648:Mesoderm / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism*; D014018:Tissue Distribution; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D021122:Protein Subunits; D015534:Trans-Activators; D014157:Transcription Factors; C086469:brm protein, Drosophila; D000251:Adenosine Triphosphatases; C084108:SMARCA4 protein, human; D004265:DNA Helicases