title: Role for A kinase-anchoring proteins (AKAPS) in glutamate receptor trafficking and long term synaptic depression.
abstract: Expression of N-methyl d-aspartate (NMDA) receptor-dependent homosynaptic long term depression at synapses in the hippocampus and neocortex requires the persistent dephosphorylation of postsynaptic protein kinase A substrates. An attractive mechanism for expression of long term depression is the loss of surface AMPA (alpha-amino-3-hydroxy-5-methylisoxazale-4-propionate) receptors at synapses. Here we show that a threshold level of NMDA receptor activation must be exceeded to trigger a stable loss of AMPA receptors from the surface of cultured hippocampal neurons. NMDA also causes displacement of protein kinase A from the synapse, and inhibiting protein kinase A (PKA) activity mimics the NMDA-induced loss of surface AMPA receptors. PKA is targeted to the synapse by an interaction with the A kinase-anchoring protein, AKAP79/150. Disruption of the PKA-AKAP interaction is sufficient to cause a long-lasting reduction in synaptic AMPA receptors in cultured neurons. In addition, we demonstrate in hippocampal slices that displacement of PKA from AKADs occludes synaptically induced long term depression. These data indicate that synaptic anchoring of PKA through association with AKAPs plays an important role in the regulation of AMPA receptor surface expression and synaptic plasticity.
authors.affiliation: Howard Hughes Medical Institute, The Picower Center for Learning and Memory and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Snyder
authors.affiliation: Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239.
authors.forename: Marcie
authors.initials: M
authors.lastname: Colledge
authors.affiliation: Howard Hughes Medical Institute, The Picower Center for Learning and Memory and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Crozier
authors.affiliation: Howard Hughes Medical Institute, The Picower Center for Learning and Memory and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
authors.forename: Wendy S
authors.initials: WS
authors.lastname: Chen
authors.affiliation: Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239.
authors.forename: John D
authors.initials: JD
authors.lastname: Scott
authors.affiliation: Howard Hughes Medical Institute, The Picower Center for Learning and Memory and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
authors.forename: Mark F
authors.initials: MF
authors.lastname: Bear
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000502:physiology*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004594:Electrophysiology; D006624:Hippocampus / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D036881:Long-Term Synaptic Depression; D019579:Neocortex / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats; D020318:Rats, Long-Evans; D018091:Receptors, AMPA / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism*; D013997:Time Factors
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017868:Cyclic AMP-Dependent Protein Kinases
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