title: Short-term very low calorie diet reduces oxidative stress in obese type 2 diabetic patients.
abstract: Oxidative stress is higher in obese diabetic than in non-diabetic subjects. This pilot study evaluates oxidative stress during short-term administration of a very low calorie diet in obese persons. Nine obese Type 2 diabetic patients (age 55+/-5 years, BMI 35.9+/-1.9 kg/m2) and nine obese non-diabetic control subjects (age 52+/-6 years, BMI 37.3+/-2.1 kg/m2) were treated by a very low calorie diet (600 kcal daily) during 8 days stay in the hospital. Serum cholesterol, triglycerides, non-esterified fatty acids (NEFA), beta-hydroxybutyrate (B-HB), ascorbic acid (AA), alpha-tocopherol (AT), plasma malondialdehyde (MDA) and superoxide dismutase (SOD) activity in erythrocytes were measured before and on day 3 and 8 of very low calorie diet administration. A decrease of serum cholesterol and triglyceride concentrations on day 8 was associated with a significant increase of NEFA (0.30+/-0.13 vs. 0.47+/-0.11 micromol/l, p<0.001) and B-HB (0.36+/-.13 vs. 2.23+/-1.00 mmol/l, p<0.001) in controls but only of B-HB (1.11+/-0.72 vs. 3.02+/-1.95 mmol/l, p<0.001) in diabetic patients. A significant decrease of plasma MDA and serum AT together with an increase of SOD activity and AA concentration (p<0.01) was observed in control persons, whereas an increase of SOD activity (p<0.01) was only found in diabetic patients after one week of the very low calorie diet. There was a significant correlation between NEFA or B-HB and SOD activity (p<0.01). We conclude that one week of a very low calorie diet administration decreases oxidative stress in obese non-diabetic but only partly in diabetic persons. Diabetes mellitus causes a greater resistance to the effects of a low calorie diet on oxidative stress.
authors.affiliation: Third Department of Internal Medicine, First Faculty of Medicine, Charles University, U nemocnice 1, 128 08 Prague 2, Czech Republic. jan.skrha@lf1.cuni.cz
authors.forename: J
authors.initials: J
authors.lastname: Skrha
authors.forename: M
authors.initials: M
authors.lastname: Kunesová
authors.forename: J
authors.initials: J
authors.lastname: Hilgertová
authors.forename: H
authors.initials: H
authors.lastname: Weiserová
authors.forename: J
authors.initials: J
authors.lastname: Krízová
authors.forename: E
authors.initials: E
authors.lastname: Kotrlíková
mesh_terms: D000328:Adult; D000368:Aged; D001205:Ascorbic Acid / Q000097:blood; D015415:Biomarkers; D031204:Caloric Restriction; D003924:Diabetes Mellitus, Type 2 / Q000178:diet therapy* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy* / Q000378:metabolism*; D018384:Oxidative Stress; D010865:Pilot Projects; D013482:Superoxide Dismutase / Q000097:blood; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol; D001205:Ascorbic Acid