title: Polymerase chain reaction amplification as a diagnostic tool in culture-negative multiple-valve endocarditis.
abstract: We report a case of culture-negative infectious endocarditis in a 17-year-old boy in which the etiologic diagnosis could only be provided by polymerase chain reaction amplification and sequencing of the bacterial 16S rRNA gene from valve tissue.
authors.affiliation: Department of Cardiothoracic and Vascular Surgery, Friedrich-Schiller University Jena, Jena, Germany.
authors.forename: Navid
authors.initials: N
authors.lastname: Madershahian
authors.forename: Justus T
authors.initials: JT
authors.lastname: Strauch
authors.forename: Martin
authors.initials: M
authors.lastname: Breuer
authors.forename: Raimund
authors.initials: R
authors.lastname: Bruhin
authors.forename: Eberhard
authors.initials: E
authors.lastname: Straube
authors.forename: Thorsten
authors.initials: T
authors.lastname: Wahlers
mesh_terms: D000293:Adolescent; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology*; D005188:False Negative Reactions; D006349:Heart Valve Diseases / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D015994:Incidence; D008297:Male; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013290:Streptococcal Infections / Q000175:diagnosis*; D034363:Viridans Streptococci / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S