title: Approaching the self-incompatibility locus Z in rye (Secale cereale L.) via comparative genetics.
abstract: Using barley and wheat expressed sequence tags as well as rice genomic sequence and mapping information, we revisited the genomic region encompassing the self-incompatibility (SI) locus Z on rye chromosome 2RL applying a comparative approach. We were able to arrange 12 novel sequence-tagged site (STS) markers around Z, spanning a genetic distance of 32.3 cM, with the closest flanking markers mapping at a distance of 0.5 cM and 1.0 cM from Z, respectively, and one marker cosegregating with Z, in a testcross population of 204 progeny. Two overlapping rice bacterial artifical chromosomes (BACs), OSJNBa0070O11 and OSJNBa0010D21, were found to carry rice orthologs of the three rye STS markers from the 1.5-cM interval encompassing Z. The STS-marker orthologs on these rice BACs span less than 125,000 bp of the rice genome. The STS marker TC116908 cosegregated with Z in a mapping population and revealed a high degree of polymorphism among a random sample of rye plants of various origin. TC116908 was shown via Southern hybridization to correspond to gene no. 10 (OSJNBa0070O11.10) on rice BAC OSJNBa0070O11. Reverse transcription-PCR with a TC116908-specific primer pair resulted in the amplification of a fragment of the expected size from the rye pistil but not from leaf cDNA. OSJNBa0070O11.10 was found to show a highly significant sequence similarity to AtUBP22, a ubiquitin-specific protease (UBP). TC116908 likely represents a putative UBP gene that is specifically expressed in rye pistils and cosegregates with Z. Given that the ubiquitination of proteins is emerging as a general mechanism involved in different SI systems of plants, TC116908 appears to be a promising target for further investigation with respect to its relation to the SI system of the grasses.
authors.affiliation: Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Rudolf-Schick-Platz 3a, 18190, Gross Lüsewitz, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hackauf
authors.forename: P
authors.initials: P
authors.lastname: Wehling
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D019295:Computational Biology; D003433:Crosses, Genetic; D017931:DNA Primers; D010450:Endopeptidases / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D012275:Oryza / Q000235:genetics; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012434:Secale / Q000235:genetics*; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics; D064570:Ubiquitin-Specific Proteases; D003313:Zea mays / Q000235:genetics
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D010450:Endopeptidases; D064570:Ubiquitin-Specific Proteases
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