Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography.
A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai was successfully established by using chloroform-methanol-water (4:3.5:2, v/v) as the two-phase solvent system. The method yielded 11.4 mg of epimedokoreanoside I, 46.5 mg of icariin and 17.7 mg of icariside II from 200 mg of the crude sample in one-step separation with the purity of 98.2%, 99.7% and 98.5%, respectively, as determined by high-performance liquid chromatography (HPLC). The structures of the flavonoids were identified by 1H NMR and 13C NMR.
Liu
Renmin
R
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Li
Aifeng
A
Sun
Ailing
A
Cui
Jichun
J
Kong
Lingyi
L
D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy
D004365:Drugs, Chinese Herbal; D005419:Flavonoids