title: The binding of copper ions to glycine-rich proteins (GRPs) from Cicer arietinum.
abstract: Cicer arietinum GRP1 and GRP2 are rich in glycine interposed with histidine and tyrosine. In order to study whether or not these proteins bind Cu(2+), circular dichroism (CD) and nuclear magnetic resonance (NMR) were measured for three synthetic peptides corresponding to sections of the protein's sequences including 1, N(1)Y(2)G(3)H(4)G(5)G(6)G(7)N(8)Y(9)G(10)N(11), where all peptides were chemically blocked with an acetyl group at the N-terminus and an -NH(2) group at the C-terminus. The visible CD spectra for 1 showed a positive peak near 590 nm not at pH 6.0 but pH 7.4 in the presence of copper ions. The Cu(2+) binding induced a drastic change in the far-UV CD spectra, showing the occurrence of large conformation changes. In the 2D TOCSY NMR spectra at pH 7.4, the addition of small amounts of CuSO(4) caused a significant broadening of proton resonances of not only His4 but also Gly5, Asn8 and Asn11. CD titration experiment suggested that NYGHGGGNYGN including one repeat unit comprises the fundamental Cu(2+) binding unit.
authors.affiliation: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Kamiya
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kumaki
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Nitta
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Kunio
authors.initials: K
authors.lastname: Hikichi
authors.forename: Norio
authors.initials: N
authors.lastname: Matsushima
mesh_terms: D000595:Amino Acid Sequence; D029863:Cicer / Q000737:chemistry*; D002942:Circular Dichroism; D003300:Copper / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D007477:Ions / Q000378:metabolism*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D007477:Ions; D010940:Plant Proteins; D003300:Copper; D005998:Glycine