SNP typing strategies.
Single nucleotide polymorphism (SNP) typing is likely to be an adjunct forensic technology to be used when short tandem repeat (STR) typing fails to yield a result or when only a partial profile is obtained. It is anticipated that forensic SNP analyses will be applied to typing mtDNA, Y chromosome lineage analyses, characterizing highly degraded DNA samples, assessing biogeographical ancestry, and typing for determining physical characteristics. As examples, three SNP typing strategies are briefly described. They are SNaP shot, SNPstream Ultra High Throughput System, and electrospray ionization mass spectrometry. The latter approach offers certain advantages for SNP analyses, particularly so for mtDNA typing.
Budowle
Bruce
B
FBI Laboratory, Quantico, VA 22135, USA. bruce.budowle@ic.fbi.gov
D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004272:DNA, Mitochondrial