Expression of the human FUSED protein in Drosophila.
The Drosophila segment polarity gene fused, which encodes a serine threonine kinase, is required to transmit the Hedgehog (Hh) signal in imaginal discs. To explore the functional homology between the human protein FUSED (hFU) and the Drosophila protein fused (dFu), we have subjected hFU to a precise and well-defined Hh signalling assay of Drosophila wing development. In the wildtype, hFU affects the expression of Hh target genes leading thus to defects in adult wings. In fu mutants, overexpression of hFU cannot rescue the fu phenotype. These results suggest that hFU in Drosophila interferes with endogenous Hh signalling probably by competing with endogenous dFu when binding its partners but cannot perform the normal Fu function.
Daoud
Fatma
F
Laboratoire de Génétique du Développement et Evolution, Institut J. Monod, CNRS UMR 7592, Université Pierre et Marie Curie, 75251 Paris cedex 05, France.
Blanchet-Tournier
Marie-Françoise
MF
D000818:Animals; D019521:Body Patterning; D002478:Cells, Cultured; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009154:Mutation; D041681:NIH 3T3 Cells; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D014162:Transfection; D019076:Transgenes; D014921:Wings, Animal / Q000378:metabolism
D019476:Insect Proteins; D011994:Recombinant Proteins; D017346:Protein Serine-Threonine Kinases; C496588:STK36 protein, human