title: Facilitated production of secretory IgA against Shiga toxin B subunits by intranasal application of antigen-coated polystyrene microspheres.
abstract: We examined the effects of microspheres as antigen carriers in mucosal immunization. Shiga toxin B subunits (Stx1B) were adsorbed on 6 mum polystyrene microspheres, which were then intranasally administered to mice together with cholera toxin (CT). Stx1B-specific serum IgG production and secretory IgA production at local mucosal sites were enhanced by the use of microspheres. When OVA was used as a model antigen, secretory IgA production but not serum IgG production was enhanced on the use of microspheres. These results indicated that microspheres provide a useful means of potentiating the immune response against Stx1B with weak immunogenicity.
authors.affiliation: Department of Microbiology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Japan.
authors.forename: Kohta
authors.initials: K
authors.lastname: Kurohane
authors.forename: Chie
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Imai
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D004337:Drug Carriers / Q000008:administration & dosage*; D004927:Escherichia coli Infections / Q000517:prevention & control; D019453:Escherichia coli O157; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000032:analysis / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009297:Nasal Mucosa / Q000276:immunology*; D010316:Particle Size; D011137:Polystyrenes; D022603:Shiga Toxins / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D004337:Drug Carriers; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D011137:Polystyrenes; D022603:Shiga Toxins; C077239:stxB toxin