In vitro and in vivo analysis of the interaction between 5.8S rRNA and ARF protein reveal a new difference between murine p19ARF and human p14ARF.
Both human and murine ARF proteins have been recently reported to impair rRNA maturation and ribosomes biogenesis through a p53-independent pathway. A specific interaction has been established between 5.8S rRNA and the murine p19ARF specie. We report here, by use of both in vitro and ChIP-RNA assays, the absence of any interaction between the human p14ARF and the homologous 5.8S rRNA. Our data are not consistent with the involvement of a 5.8S-p14ARF complex in ribosome biogenesis in man. Rather they suggest that the human protein does not require such an interaction to achieve a similar function. This result is a new argument in favour of functional differences between human and murine ARF proteins.
Andrique
Laëtitia
L
EA 3805, Laboratoire d'Oncologie Moléculaire, Pôle Biologie-Santé, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Ayrault
Olivier
O
Larsen
Christian-Jacques
CJ
Séité
Paule
P
D000818:Animals; D019941:Cyclin-Dependent Kinase Inhibitor p16; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000378:metabolism*; D025803:Tumor Suppressor Protein p14ARF / Q000494:pharmacology*
C417743:Cdkn2a protein, mouse; D019941:Cyclin-Dependent Kinase Inhibitor p16; D012340:RNA, Ribosomal, 5.8S; D025803:Tumor Suppressor Protein p14ARF