Dimethylsulfoxide induces upregulation of tumor suppressor protein PTEN through nuclear factor-kappaB activation in HL-60 cells.
Dimethylsulfoxide (DMSO) has been known to differentiate HL60 cells into neutrophil like cells. Here, we provide an evidence for the involvement of tumor suppressor PTEN, an antagonist of phosphatidylinositol 3-kinase (PI3K) in the DMSO-induced differentiation of HL60 cells. DMSO upregulated PTEN with unaffecting the expression of PI3K. The upregulation of PTEN by DMSO lead to the decrease of Akt phosphorylation, a downstream of PI3K. The DMSO-induced upregulation of PTEN might be mediated by NF-kappaB activation, which was evidenced by the blockage of DMSO-induced PTEN upregulation with an NF-kappaB inhibitor, pyrrolidine dithiocarbamate (PDTC).
Lee
Young-Rae
YR
Department of Biochemistry, Chonbuk National University Medical School, Chonju 560-182, Republic of Korea.
Shim
Hyun-Jaung
HJ
Yu
Hong-Nu
HN
Song
Eun-Kyung
EK
Park
Jinny
J
Kwon
Kang-Beom
KB
Park
Jin-Woo
JW
Rho
Hye-Won
HW
Park
Byung-Hyun
BH
Han
Myung-Kwan
MK
Kim
Jong-Suk
JS
D001483:Base Sequence; D017931:DNA Primers; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016147:Genes, Tumor Suppressor / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D016328:NF-kappa B / Q000378:metabolism*; D051059:PTEN Phosphohydrolase; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D017931:DNA Primers; D016328:NF-kappa B; D000081082:Phosphoinositide-3 Kinase Inhibitors; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D004121:Dimethyl Sulfoxide