title: Relationship between physical, biochemical and biomechanical properties of tissue-engineered cartilage-carrier-constructs.
abstract: Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. Physical parameters (height and weight) correlated very well with total DNA content (r2 = 0.86, re. 0.94). The relation between DNA content and glycosaminoglycan content was less but still significant. No significant relationship was found between the elasticity module and the DNA content, even if the elasticity module increased slightly at higher DNA content. With respect to later implantation, selection of a construct for implantation based on the weight, which can be determined non-invasive and under sterile conditions, seems to be justifiable.
authors.affiliation: Bioprocess and Biochemical Engineering, Hamburg, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Nagel-Heyer
authors.forename: Christiane
authors.initials: C
authors.lastname: Goepfert
authors.forename: Michael M
authors.initials: MM
authors.lastname: Morlock
authors.forename: Ralf
authors.initials: R
authors.lastname: Pörtner
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002356:Cartilage / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000166:cytology; D004247:DNA / Q000032:analysis; D004548:Elasticity; D006025:Glycosaminoglycans / Q000032:analysis; D012044:Regression Analysis; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D006025:Glycosaminoglycans; D004247:DNA