An easy approach for the purification of native TFIIH.
Transcriptional regulation depends on the appropriate set of positive and negative regulating signals in order to provide the correct gene expression. In vitro studies in eukaryotic gene expression over the last few years have provided a wealth of information about new factors involved in the regulation of genes. However, the dissection of this mechanism requires the addition of well-characterized general transcription factors; with the exception of TFIID and TFIIH, all others can easily be expressed in a recombinant form. Here we provide a simple methodology to obtain partially purified transcriptionally active TFIIH free from other general transcription factors and active in transcription.
Castaño
Enrique
E
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, México. enriquec@cicy.mx
Flores
Ruben Dario
RD
Zapata
Luis Carlos Rodriguez
LC
D002852:Chromatography, Ion Exchange; D006367:HeLa Cells; D006801:Humans; D011489:Protein Denaturation; D051758:Transcription Factor TFIIH; D019473:Transcription Factors, TFII / Q000737:chemistry / Q000302:isolation & purification*
D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH