"Waste" follicular aspirate from fertility treatment--a potential source of human germline stem cells?
Fertility clinics worldwide routinely produce a large volume of 'waste' follicular aspirate, which is potentially an abundant source of immature ovarian follicles. Current attempts to cultivate these further in vitro to yield viable mature oocytes for fertility treatment have not yet achieved much success. Instead, recent lines of evidence have emerged that are suggestive of a potential stem cell niche within such immature ovarian follicles. The recent discovery of follicular renewal and putative germ-line stem cells within the postnatal mammalian ovary shook the foundations of reproductive biology by challenging the established dogma that mammalian females lose the capacity for germ cell renewal during fetal life, such that a fixed reserve of germ cells (oocytes) enclosed within follicles is endowed at birth. More intriguingly, another recent study in the Drosophila model provided compelling evidence that somatic progenies (nurse cells) of germ-line stem cells had the ability to revert back to the stem-cell-like state. This introduces the exciting possibility that within the mammalian ovarian follicle, similar somatic progenies of germ-line stem cells may also possess a greater intrinsic ability to revert back into functional stem cells. If this is the case, then a favored candidate would be the cumulus/granulosa of immature ovarian follicles, since such cells are true homologues of nurse cells found within the Drosophila ovary. The successful elucidation of a human germ-line stem cell niche within immature ovarian follicles is likely to have huge ramifications in stem cell biology and regenerative medicine.
Heng
Boon Chin
BC
Stem Cell Laboratory, Faculty of Dentistry, National University of Singapore, Singapore 119074.
Cao
Tong
T
Bested
Soren Müller
SM
Tong
Guo Qing
GQ
Ng
Soon Chye
SC
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D006080:Ovarian Follicle / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*