title: Automated nanospray using chip-based emitters for the quantitative analysis of pharmaceutical compounds.
abstract: An automated nanospray system based on chip technology (the NanoMate) was successfully interfaced to a modified Particle Discriminator Interface on a triple quadrupole mass spectrometer. A number of the interface parameters were optimized to improve the sampling efficiency for ions from the chip-based system. Analytical performance was assessed using a number of biochemicals as well as via a methodology for a pharmaceutical that passed validation as required by Good Laboratory Practices. Infusion analyses in flow rates <1 microL/min provided advantages in terms of throughput and sample consumption when compared to other methodologies based on liquid chromatography.
authors.affiliation: Eli Lilly Canada Inc., Toronto, Ontario, Canada.
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Corkery
authors.forename: Henrianna
authors.initials: H
authors.lastname: Pang
authors.forename: Bradley B
authors.initials: BB
authors.lastname: Schneider
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Covey
authors.forename: K W Michael
authors.initials: KW
authors.lastname: Siu
mesh_terms: D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis; D046208:Microchip Analytical Procedures / Q000379:methods*; D036103:Nanotechnology; D004364:Pharmaceutical Preparations / Q000032:analysis*; D043562:Receptors, G-Protein-Coupled / Q000032:analysis; D054440:Receptors, Ghrelin; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D000093542:Gemcitabine
chemical_list: D004364:Pharmaceutical Preparations; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin; D003841:Deoxycytidine; D000093542:Gemcitabine
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