Rejuvenation of aged progenitor cells by exposure to a young systemic environment.
The decline of tissue regenerative potential is a hallmark of ageing and may be due to age-related changes in tissue-specific stem cells. A decline in skeletal muscle stem cell (satellite cell) activity due to a loss of Notch signalling results in impaired regeneration of aged muscle. The decline in hepatic progenitor cell proliferation owing to the formation of a complex involving cEBP-alpha and the chromatin remodelling factor brahma (Brm) inhibits the regenerative capacity of aged liver. To examine the influence of systemic factors on aged progenitor cells from these tissues, we established parabiotic pairings (that is, a shared circulatory system) between young and old mice (heterochronic parabioses), exposing old mice to factors present in young serum. Notably, heterochronic parabiosis restored the activation of Notch signalling as well as the proliferation and regenerative capacity of aged satellite cells. The exposure of satellite cells from old mice to young serum enhanced the expression of the Notch ligand (Delta), increased Notch activation, and enhanced proliferation in vitro. Furthermore, heterochronic parabiosis increased aged hepatocyte proliferation and restored the cEBP-alpha complex to levels seen in young animals. These results suggest that the age-related decline of progenitor cell activity can be modulated by systemic factors that change with age.
Conboy
Irina M
IM
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California 94305, USA.
Conboy
Michael J
MJ
Wagers
Amy J
AJ
Girma
Eric R
ER
Weissman
Irving L
IL
Rando
Thomas A
TA
D000375:Aging / Q000502:physiology*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000502:physiology; D029721:Drosophila Proteins; D005110:Extracellular Space / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000502:physiology; D008115:Liver Regeneration / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000166:cytology / Q000293:injuries / Q000473:pathology / Q000502:physiology; D009928:Organ Specificity; D051880:Receptors, Notch; D012038:Regeneration / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism
D022763:CCAAT-Enhancer-Binding Protein-alpha; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; D051880:Receptors, Notch; D015534:Trans-Activators; C086469:brm protein, Drosophila