Phenotypic and genetic changes in coxsackievirus B5 following repeated passage in mouse pancreas in vivo.
Common enterovirus infections appear to initiate or facilitate the pathogenetic processes leading to type 1 diabetes, and also sometimes precipitate the clinical disease. In experimental infection of mice, coxsackieviruses have shown to have a strong affinity for the exocrine tissue, while even in lethal cases, the islets remain unaffected. The virus strain most intensively studied in this respect is the diabetogenic variant E2 of coxsackievirus B4. In addition, it is known that all six serotypes of coxsackie B viruses can be made diabetogenic by repeated passages in either mouse pancreas in vivo or in cultured mouse beta-cells in vitro. However, the genetic determinants of the phenomenon have not been determined. In the present study, a laboratory strain of coxsackievirus B5 was passaged repeatedly in mouse pancreas in vivo. After 15 passages, the virus phenotype was clearly changed and infection of the variant resulted in a diabetes-like syndrome in mice characterized by chronic pancreatic inflammation together with dysregulation in glucose metabolism, loss of pancreatic acinar tissue, and mild insulitis. In order to characterize the genetic determinants involved in mouse pancreas adaptation, the passaged virus variant together with the parental virus strain was cloned for molecular characterization. The whole genome sequencing of both virus strains revealed only limited differences. Altogether, eight nucleotides were changed resulting in five amino acid substitutions, of which three were located in the capsid proteins.
Al-Hello
Haider
H
Enterovirus Laboratory, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
Davydova
Berta
B
Smura
Teemu
T
Kaialainen
Svetlana
S
Ylipaasto
Petri
P
Saario
Elise
E
Hovi
Tapani
T
Rieder
Elizabeth
E
Roivainen
Merja
M
D000818:Animals; D001786:Blood Glucose; D002908:Chronic Disease; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000276:immunology / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D005838:Genotype; D007249:Inflammation; D008297:Male; D051379:Mice; D010179:Pancreas / Q000276:immunology / Q000473:pathology / Q000821:virology*; D010641:Phenotype; D012692:Serial Passage
D001786:Blood Glucose