title: Preparation of Z-L-Phe-OH-NBD imprinted microchannel and its molecular recognition study.
abstract: An integrated microchip was presented for selective recognition of Z-L-Phe-OH-NBD, using molecular imprinting technique. Molecularly imprinted polymer (MIP) were prepared by copolymerization in the presence of template molecule Z-L-Phe-OH-NBD, in which methacrylic acid and 4-vinylpyridine were used as functional monomers and ethylene dimethacrylate used as crosslinker. Imprinted polymer particles were introduced into a microchannel fabricated with a new material i.e. poly(methylvinylsiloxane) by simply rapid prototyping method. Imprinted effects were evaluated by laser-induced fluorescence (LIF) detection where the results indicated that good selective recognition for Z-L-Phe-OH-NBD in the imprinted polymer was obtained; the adsorption percentage of Z-L-Phe-OH-NBD was 61%. In contrast to conventional molecular imprinting analysis, integration shortened overall analysis time from 4h to 10 min.
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Jian-Du
authors.initials: JD
authors.lastname: Lei
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Tong
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D018716:Molecular Mimicry; D036103:Nanotechnology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D012833:Siloxanes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008689:Methacrylates; D011108:Polymers; D011145:Polyvinyls; D011725:Pyridines; D012833:Siloxanes; C008384:methacrylic acid; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; C014766:polymethylvinylsiloxane; D009327:4-Chloro-7-nitrobenzofurazan; C029351:4-vinylpyridine