Culture of neural cells on polymers coated surfaces for biosensor applications.
We focused our study on the olfactory cells growth on biocompatible polymer films electrodeposited on a silicon microsystem. Several substrates such as polyethyleneimine (PEI), polypropyleneimine (PPI), and polypyrrole (PPy), acting as potentially good candidates for cell culture, were tested in order to allow cells to adhere and proliferate. During their growth, the evolution of their morphology was monitored using both confocal microscope and immunohistochemistry, leading to the conclusion of a normal development. An estimation of the adhesion and proliferation rates of rat neuronal cell cultures indicated that PEI and PPI were the best substrates for cultivating olfactory cells.
Lakard
S
S
Laboratory of Chemistry, Materials and Interfaces, University of Franche-Comté, 16 route de Gray, 25030 Besançon, France. sophie.lakard@univ-fcomte.fr
Herlem
G
G
Valles-Villareal
N
N
Michel
G
G
Propper
A
A
Gharbi
T
T
Fahys
B
B
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology*; D011108:Polymers / Q000737:chemistry*; D051381:Rats
D020099:Coated Materials, Biocompatible; D011108:Polymers