title: The metal permease ZupT from Escherichia coli is a transporter with a broad substrate spectrum.
abstract: The Escherichia coli zupT (formerly ygiE) gene encodes a cytoplasmic membrane protein (ZupT) related to members of the eukaryotic ZIP family of divalent metal ion transporters. Previously, ZupT was shown to be responsible for uptake of zinc. In this study, we show that ZupT is a divalent metal cation transporter of broad substrate specificity. An E. coli strain with a disruption in all known iron uptake systems could grow in the presence of chelators only if zupT was expressed. Heterologous expression of Arabidopsis thaliana ZIP1 could also alleviate iron deficiency in this E. coli strain, as could expression of indigenous mntH or feoABC. Transport studies with intact cells showed that ZupT facilitates uptake of 55Fe2+ similarly to uptake of MntH or Feo. Other divalent cations were also taken up by ZupT, as shown using 57Co2+. Expression of zupT rendered E. coli cells hypersensitive to Co2+ and sensitive to Mn2+. ZupT did not appear to be metal regulated: expression of a Phi(zupT-lacZ) operon fusion indicated that zupT is expressed constitutively at a low level.
authors.affiliation: Institut für Mikrobiologie, Martin-Luther-Universität, Halle, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Grass
authors.forename: Sylvia
authors.initials: S
authors.lastname: Franke
authors.forename: Nadine
authors.initials: N
authors.lastname: Taudte
authors.forename: Dietrich H
authors.initials: DH
authors.lastname: Nies
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Kucharski
authors.forename: Michael E
authors.initials: ME
authors.lastname: Maguire
authors.forename: Christopher
authors.initials: C
authors.lastname: Rensing
mesh_terms: D003035:Cobalt / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D019216:Metals, Heavy / Q000378:metabolism*; D009154:Mutation; D013379:Substrate Specificity / Q000502:physiology; D015032:Zinc / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D019216:Metals, Heavy; C497880:ZupT protein, E coli; D003035:Cobalt; D008345:Manganese; D007501:Iron; D015032:Zinc
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