title: Use of a biphasic graft constructed with chondrocytes overlying a beta-tricalcium phosphate block in the treatment of rabbit osteochondral defects.
abstract: To evaluate the ability of a biphasic construct to repair osteochondral defects in articular cartilage, plugs made of chondrocytes in collagen gel overlying a resorbable porous beta-tricalcium phosphate (TCP) block were implanted into defects in rabbit knees. The repair tissue was evaluated at 8, 12, and 30 weeks. Eight weeks after implantation of the biphasic construct, histologic examination showed hyaline-like cartilage formation that was positive for safranin O and type II collagen. At 12 weeks, most of the beta-TCP was replaced by bone, with a small amount remaining in the underlying cartilage. In the cell-seeded layer, the newly formed middle and deep cartilage adjacent to the subchondral bone stained with safranin O, but no staining was observed in the superficial layer. In addition, cell morphology was distinctly different from the deep levels of the reparative cartilage, with hypertrophic cells at the bottom of the cartilaginous layer. At 30 weeks, beta-TCP had completely resorbed and a tidemark was observed in some areas. In contrast, controls (defects filled with a beta-TCP block alone) showed no cartilage formation but instead had subchondral bone formation. These findings indicate that beta-TCP-supported chondrocytes in collagen gel can partially repair isolated articular cartilage osteochondral defects.
authors.affiliation: Department of Orthopedic Surgery, NHO Utsunomiya National Hospital, Tochigi, Japan. tanakat@e-utunomiya.hosp.go.jp
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Komaki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Chazono
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Fujii
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001842:Bone and Bones / Q000502:physiology; D002130:Calcium Phosphates / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000473:pathology / Q000637:transplantation*; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D002516:Ceramics; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000637:transplantation*; D003094:Collagen / Q000737:chemistry; D024043:Collagen Type II / Q000378:metabolism; D005782:Gels / Q000737:chemistry; D020007:Implants, Experimental; D007592:Joint Diseases / Q000473:pathology / Q000601:surgery*; D007719:Knee Joint / Q000473:pathology / Q000601:surgery*; D011817:Rabbits; D013997:Time Factors; D023822:Tissue Engineering; D014184:Transplantation, Homologous
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D024043:Collagen Type II; D005782:Gels; D003094:Collagen; C018392:tricalcium phosphate