title: The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation.
abstract: It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547-1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous "precentrioles" become morphologically recognizable centrioles before mitosis. De novo-assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo-formed centrioles do not mature if they are assembled in S phase-arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway.
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA.
authors.forename: Sabrina
authors.initials: S
authors.lastname: La Terra
authors.forename: Christopher N
authors.initials: CN
authors.lastname: English
authors.forename: Polla
authors.initials: P
authors.lastname: Hergert
authors.forename: Bruce F
authors.initials: BF
authors.lastname: McEwen
authors.forename: Greenfield
authors.initials: G
authors.lastname: Sluder
authors.forename: Alexey
authors.initials: A
authors.lastname: Khodjakov
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D002502:Centrioles / Q000502:physiology*; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D016196:S Phase / Q000502:physiology
references.citation: Biochim Biophys Acta. 1996 Oct 9;1288(2):F55-78
references.pmid: 8876633
references.citation: J Cell Biol. 1982 Jun;93(3):938-49
references.pmid: 7119006
references.citation: Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9141-6
references.pmid: 9256449
references.citation: Cell Motil Cytoskeleton. 1997;38(4):311-7
references.pmid: 9415373
references.citation: Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9295-300
references.pmid: 9689074
references.citation: Cancer Res. 1998 Sep 1;58(17):3974-85
references.pmid: 9731511
references.citation: J Cell Biol. 1998 Dec 14;143(6):1575-89
references.pmid: 9852152
references.citation: Cell Motil Cytoskeleton. 1998;41(4):281-8
references.pmid: 9858153
references.citation: Methods Cell Biol. 1999;61:81-111
references.pmid: 9891310
references.citation: Methods Cell Biol. 1999;61:297-315
references.pmid: 9891321
references.citation: Biol Cell. 1999 Jul;91(6):451-60
references.pmid: 10519005
references.citation: Curr Biol. 2000 Jan 27;10(2):59-67
references.pmid: 10662665
references.citation: J Cell Biol. 2000 Apr 17;149(2):317-30
references.pmid: 10769025
references.citation: Trends Cell Biol. 2001 Jan;11(1):18-21
references.pmid: 11146294
references.citation: Science. 2001 Feb 23;291(5508):1547-50
references.pmid: 11222860
references.citation: Science. 2001 Feb 23;291(5508):1550-3
references.pmid: 11222861
references.citation: Curr Biol. 2001 Mar 6;11(5):308-17
references.pmid: 11267867
references.citation: J Cell Biol. 2001 Apr 2;153(1):237-42
references.pmid: 11285289
references.citation: Trends Cell Biol. 2001 Oct;11(10):413-9
references.pmid: 11567874
references.citation: Cell Motil Cytoskeleton. 2002 Jun;52(2):66-81
references.pmid: 12112149
references.citation: Oncogene. 2002 Sep 9;21(40):6234-40
references.pmid: 12214254
references.citation: J Cell Biol. 2002 Sep 30;158(7):1171-81
references.pmid: 12356862
references.citation: Nat Rev Cancer. 2002 Nov;2(11):815-25
references.pmid: 12415252
references.citation: J Cell Biol. 2002 Dec 9;159(5):731-7
references.pmid: 12473683
references.citation: Nat Cell Biol. 2003 Jun;5(6):539-44
references.pmid: 12766773
references.citation: Dev Biol. 2003 Aug 15;260(2):298-313
references.pmid: 12921733
references.citation: J Cell Biol. 2003 Sep 29;162(7):1211-21
references.pmid: 14504268
references.citation: Eur J Cell Biol. 1982 Aug;28(1):68-76
references.pmid: 7128618
references.citation: Cell Motil Cytoskeleton. 1986;6(4):355-62
references.pmid: 2875802
references.citation: Int Rev Cytol. 1987;100:49-92
references.pmid: 3549609
references.citation: Science. 1989 Nov 3;246(4930):629-34
references.pmid: 2683079
references.citation: Cell. 1991 Nov 1;67(3):495-504
references.pmid: 1934057
references.citation: Biol Cell. 1991;72(1-2):61-6
references.pmid: 1756312
references.citation: Science. 1992 Apr 10;256(5054):219-21
references.pmid: 1566068
references.citation: Nature. 1995 Dec 7;378(6557):578-83
references.pmid: 8524390
references.citation: Nature. 1995 Dec 7;378(6557):638-40
references.pmid: 8524401
references.citation: J Cell Sci. 1972 Sep;11(2):521-41
references.pmid: 5076360
references.citation: J Cell Sci. 1996 Dec;109 ( Pt 13):3089-102
references.pmid: 9004043