title: Effects of polyunsaturated fatty acids on the proliferation of mitogen stimulated bovine peripheral blood mononuclear cells.
abstract: The present study aimed at analysis of the effects of polyunsaturated fatty acid (PUFA), linoleic acid (LA, C18:2n - 6) and linolenic acid (LNA, C18:3n - 3) on bovine peripheral blood mononuclear cells (PBMC) in vitro. Both mitogen (ConA)-induced proliferative lymphocyte responsiveness during 4 days of culture and eicosanoid (prostaglandin E(2) (PGE(2)) and leukotriene B(4) (LTB(4))) production during 36 h were determined in relation to the absence or presence of various concentrations of LA and LNA (0, 1, 5, 25, 125 and 250 microM). Mitogen-driven proliferative responses of lymphocytes tended to be uninfluenced in the presence of lower concentrations of LA, whereas significant inhibition was observed at the higher concentrations of LA (125 and 250 microM). However, increasing amounts of LNA did not affect the proliferation. ConA stimulation induced a clear PGE(2) response, which significantly decreased in the presence of 250 microM of LA. In addition, increasing amounts of LNA, but not LA, led to a significant decrease in LTB(4) levels. However, The production of LTB(4) did not alter due to mitogenic stimulation. In conclusion, the present study shows that bovine mononuclear cells may functionally be influenced by the presence of PUFA in their environment. Further studies need to be conducted to clarify in vivo consequences of these findings in a situation of PUFA enriched rations in ruminants.
authors.affiliation: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, Utrecht, The Netherlands. jitkamol@yahoo.com
authors.forename: J
authors.initials: J
authors.lastname: Thanasak
authors.forename: K E
authors.initials: KE
authors.lastname: Müller
authors.forename: S J
authors.initials: SJ
authors.lastname: Dieleman
authors.forename: A
authors.initials: A
authors.lastname: Hoek
authors.forename: J P T M
authors.initials: JP
authors.lastname: Noordhuizen
authors.forename: V P M G
authors.initials: VP
authors.lastname: Rutten
mesh_terms: D000818:Animals; D002417:Cattle; D003208:Concanavalin A / Q000276:immunology; D015232:Dinoprostone / Q000032:analysis / Q000276:immunology; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D007975:Leukotriene B4 / Q000032:analysis / Q000276:immunology; D019787:Linoleic Acid / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D012588:Scintillation Counting / Q000662:veterinary; D013936:Thymidine / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000276:immunology / Q000494:pharmacology*
chemical_list: D017962:alpha-Linolenic Acid; D003208:Concanavalin A; D007975:Leukotriene B4; D019787:Linoleic Acid; D015232:Dinoprostone; D013936:Thymidine