title: Formalin hindpaw injection induces changes in the [3H]prazosin binding to alpha1-adrenoceptors in specific regions of the mouse brain and spinal cord.
abstract: Involvement of the alpha1-adrenoceptor subtypes in early and late phases of formalin pain was investigated by quantitative in vitro autoradiography in the spinal cord and brain structures of CD-1 mice. Total alpha1-adrenoceptors binding (including all alpha1-adrenoceptor subtypes) was assessed with [3H]prazosin; alpha(1B)-adrenoceptor was assessed with [3H]prazosin in the presence of 10 nM WB4101 to mask remaining alpha1-adrenoceptor subtypes. Early after formalin injection the alpha1-adrenoceptors (mainly alpha1B receptor) binding was reduced in the contralateral hind limb area of the somatosensory cortex and in the secondary motor cortex. A reduction occurred also in the ipsilateral laminae I-III of the spinal cord (both alpha1B- and non-alpha1B-adrenoceptors). Lately an increase of alpha1-adrenoceptors binding (mostly subtypes other than alpha1B) appeared in discrete amygdaloid and thalamic nuclei. These results provide the first description of changes at the level of central alpha1-adrenoceptors' binding during the formalin-induced pain in mice. Their distribution suggests that they may have a functional meaning.
authors.affiliation: Department of Biochemistry, Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland. nfnalepa@cyf-kr.edu.pl
authors.forename: I
authors.initials: I
authors.lastname: Nalepa
authors.forename: J
authors.initials: J
authors.lastname: Vetulani
authors.forename: V
authors.initials: V
authors.lastname: Borghi
authors.forename: M
authors.initials: M
authors.lastname: Kowalska
authors.forename: B
authors.initials: B
authors.lastname: Przewłocka
authors.forename: F
authors.initials: F
authors.lastname: Pavone
mesh_terms: D000679:Amygdala / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002490:Central Nervous System / Q000378:metabolism*; D006614:Hindlimb; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009044:Motor Cortex / Q000378:metabolism; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D010147:Pain Measurement; D011224:Prazosin / Q000378:metabolism* / Q000494:pharmacology; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D013003:Somatosensory Cortex / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D013788:Thalamus / Q000378:metabolism; D014316:Tritium
chemical_list: D018340:Receptors, Adrenergic, alpha-1; D014316:Tritium; D011224:Prazosin
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