Electrochromatographic evaluation of a silica monolith capillary column for separation of basic pharmaceuticals.
A silica-based monolithic capillary column was prepared via a sol-gel process. The continuous skeleton and large through-pore structure were characterized by scanning electron microscopy (SEM). The native silica monolith has been successfully employed in the electrochromatographic separation of beta-blockers and alkaloids extracted from traditional Chinese medicines (TCMs). Column efficiencies greater than 250 000 plates/m for capillary electrochromatography (CEC) separation of basic compounds were obtained. It was observed that retention of basic pharmaceuticals on the silica monolith was mainly contributed by a cation-exchange mechanism. Other retention mechanisms including reversed-phase and normal-phase mechanisms and electrophoresis of basic compounds also played a role in separation. A comparison of the differences between CEC and capillary zone electrophoresis (CZE) separation was also discussed.
Xie
Chuanhui
C
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
Hu
Jiwei
J
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.|College of Chemistry & Molecular Sciences, Wuhan University, Wuhan, China.
Xiao
Hua
H
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
Su
Xingye
X
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
Dong
Jing
J
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
Tian
Ruijun
R
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
He
Zhike
Z
College of Chemistry & Molecular Sciences, Wuhan University, Wuhan, China.
Zou
Hanfa
H
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
D000319:Adrenergic beta-Antagonists / Q000302:isolation & purification; D000470:Alkaloids / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D039323:Coptis / Q000737:chemistry; D031311:Corydalis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
D000319:Adrenergic beta-Antagonists; D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D004364:Pharmaceutical Preparations; D012822:Silicon Dioxide
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