title: Production and characterization of monoclonal antibodies against human ceruloplasmin.
abstract: Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57 %. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.
authors.affiliation: Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Choi
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Choi
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kang
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D001921:Brain / Q000276:immunology / Q000378:metabolism; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002570:Ceruloplasmin / Q000276:immunology* / Q000378:metabolism; D002645:Chickens; D003429:Cross Reactions; D004285:Dogs; D018604:Epitope Mapping; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D002570:Ceruloplasmin