title: The effectiveness of three instrumentation techniques on the elimination of Enterococcus faecalis from a root canal: an in vitro study.
abstract: The in vitro reduction of a bacterial population in a root canal by mechanical instrumentation using three techniques was evaluated. Root canals inoculated with a Enterococcus faecalis (E. faecalis) suspension were instrumented using hand Hedstroem files, Giromatic files, and Hero 642 rotary instruments. Irrigation was performed using sterile saline solution. Root canals were sampled before and after instrumentation. After serial dilutions, samples were plated onto Mitis-Salivarius agar and the colony forming units grown were counted. All instruments tested were able to significantly reduce the number of bacterial cells in the root canal, however, the results of this study indicated that Hedstroem files, Giromatic, and Hero 642 techniques were not significantly different in their ability to reduce intracanal bacteria.
authors.affiliation: Department of Endodontics of the Faculty of Dentistry at Atatürk University in Erzurum, Turkey.
authors.forename: Meltem
authors.initials: M
authors.lastname: Colak
authors.forename: Sinan
authors.initials: S
authors.lastname: Evcil
authors.forename: Y Ziya
authors.initials: YZ
authors.lastname: Bayindir
authors.forename: Nimet
authors.initials: N
authors.lastname: Yigit
mesh_terms: D001641:Bicuspid; D015169:Colony Count, Microbial; D003722:Dental Alloys; D003762:Dental Instruments; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis; D004867:Equipment Design; D006801:Humans; D009532:Nickel; D018915:Root Canal Preparation / Q000295:instrumentation* / Q000379:methods*; D013193:Stainless Steel; D014025:Titanium
chemical_list: D003722:Dental Alloys; C040654:titanium nickelide; D013193:Stainless Steel; D009532:Nickel; D014025:Titanium