title: Simultaneous infection with dengue 2 and 3 viruses in a Chinese patient return from Sri Lanka.
abstract: Dengue is an acute viral disease transmitted by the Aedes aegypti and Aedes albopictus mosquito, which are present in most tropical urban areas of the world. There are four antigenically distinct serotypes, designated dengue-1 (DEN-1), dengue-2 (DEN-2), dengue-3 (DEN-3) and dengue-4 (DEN-4). Dengue outbreaks have occurred in several regions in Asia, involving four serotypes of dengue 1, 2, 3 and 4. In review of the few cases of dual infection documented in the literature, we report here a case of simultaneous infection with DEN-2 and DEN-3 in a Chinese patient return from Sri Lanka. The dual infection was identified by type-specific indirect immunofluorescence assay and confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence determination. This is the first documented case of simultaneous infection with serotype of DEN-2 and DEN-3 in China.
authors.affiliation: Laboratory of virology, Institute of Microbiology and Epidemiology, 20 DongDa Street, Beijing 100071, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wenming
authors.forename: Yu
authors.initials: Y
authors.lastname: Man
authors.forename: Fan
authors.initials: F
authors.lastname: Baochang
authors.forename: Deng
authors.initials: D
authors.lastname: Yongqiang
authors.forename: Jiang
authors.initials: J
authors.lastname: Tao
authors.forename: Duan
authors.initials: D
authors.lastname: Hongyuan
authors.forename: Qin
authors.initials: Q
authors.lastname: Ede
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002681:China; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000302:isolation & purification; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013188:Sri Lanka; D014195:Travel
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral