[Determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC].
OBJECTIVE
To establish methods for the determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC.


METHODS
The determination of phillyrin was carried out on YWG-C18 column (4.6 mm x 250 mm, 10 microm), using acetonitrile-water (25:75) as mobile phase at a flow rate of 0.8 mL x min(-1) and detected at the wavelength 277 nm. The determination of forsythoside A was carried out with YWG-C18 column(4.6 mm x 250 mm,10 microm), using acetonitrile-water-Acetic acid (17:83:0.4) as mobile phase at a flow rate of 1.0 mL x min(-1) and detected at the wavelength 280 nm.


RESULTS
The average recovery of phillyrin was 99.6%, RSD = 1.9% (n = 5). The average recovery of forsythoside A was 101.3%, RSD = 2.5% ( n = 5).


CONCLUSIONS
The methods were simple and accurate and could be used to control the quality of the Lianqiao Bingdu Qing capsule.
Zhao
Wen-Hua
WH
Capital University of Medical Sciences, Beijing 100054, China.
Shi
Ren-Bing
RB
Liu
Bin
B
Zhang
Lan-Zhen
LZ
D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D031657:Forsythia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
D002214:Capsules; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; C058049:forsythiaside; C075528:phillyrin