Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis.
To confer the ability to ferment cello-oligosaccharides on the ethanol-producing bacterium, Zymomonas mobilis, the beta-glucosidase gene from Ruminococcus albus, tagged at its N-terminal with the 53-amino acid Tat signal peptide from the periplasmic enzyme glucose-fructose oxidoreductase from Z. mobilis, was introduced into the strain. The tag enabled 61% of the beta-glucosidase activity to be transported through the cytoplasmic membrane of the recombinant strain which then produced 0.49 g ethanol/g cellobiose.
Yanase
Hideshi
H
Department of Biotechnology, Tottori University, Tottori, Tottori, 680-8552, Japan. yanase@bio.tottori-u.ac.jp
Nozaki
Koji
K
Okamoto
Kenji
K
D002475:Cellobiose / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D045857:Ruminococcus / Q000235:genetics / Q000378:metabolism*; D017355:Zymomonas / Q000235:genetics / Q000378:metabolism*; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D002475:Cellobiose; D000431:Ethanol; D002482:Cellulose; D001617:beta-Glucosidase