Establishment of a genetically marked insect-derived symbiont in multiple host plants.
Alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (Homalodisca coagulata), was transformed using the Himar1 transposition system to express EGFP. Seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time PCR using primers designed to amplify the gene insert. The largest colony of 3,591,427 cells/2 cm of A. xylosoxidans subsp. denitrificans was found in Citrus limon, with almost all plants testing positive in both trials. The amount of colonization decreased in the other plants tested in the following order: orange (Citrus sinensis "sweet orange") > chrysanthemum (Chrysanthemum grandiflora cv. "White Diamond") > periwinkle (Vinca rosea) > crepe myrtle (Lagerstroemia indica) > grapevine (Vitis vinifera cv. Chardonnay). The bacterium's preference for citrus paralleled the host insect's preference for this same plant. Additional tests determined that A. xylosoxidans subsp. denitrificans thrives as a nonpathogenic, xylem-associated endophyte.
Bextine
Blake
B
Department of Entomology, University of California, Riverside, CA 92507, USA. blake.bextine@ucr.edu
Lampe
David
D
Lauzon
Carol
C
Jackson
Brian
B
Miller
Thomas A
TA
D000421:Alcaligenes / Q000235:genetics*; D000818:Animals; D006430:Hemiptera / Q000382:microbiology*; D007303:Insect Vectors / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013559:Symbiosis
Curr Microbiol. 1995 Dec;31(6):377-81
8528010
Appl Environ Microbiol. 1977 Feb;33(2):392-9
848958
Phytopathology. 1999 Jan;89(1):53-8
18944803
Appl Environ Microbiol. 2002 Oct;68(10):4906-14
12324338
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11428-33
10500193
Res Microbiol. 2003 Mar;154(2):105-14
12648725
Arch Microbiol. 2001 Apr;175(4):259-62
11382221
Arthropod Struct Dev. 2003 Oct;32(2-3):189-99
18089004
J Med Entomol. 2000 Nov;37(6):924-8
11126551
Nature. 1973 Aug 31;244(5418):577-80
4582514
Science. 2000 Aug 25;289(5483):1352-5
10958782
J Bacteriol. 1988 Jun;170(6):2575-83
2836362
Infect Immun. 2000 Nov;68(11):6391-7
11035750
Curr Microbiol. 2004 May;48(5):327-31
15060727
J Bacteriol. 1978 Jun;134(3):1141-56
149110
J Bacteriol. 2000 Oct;182(19):5391-8
10986241
Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11672-7
12972635
Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1645-50
9990078
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
10829079
Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3274-8
9096383