Human mesenchymal stem cells in rodent whole-embryo culture are reprogrammed to contribute to kidney tissues.
The use of stem cells has enabled the successful generation of simple organs. However, anatomically complicated organs such as the kidney have proven more refractory to stem-cell-based regenerative techniques. Given the limits of allogenic organ transplantation, an ultimate therapeutic solution is to establish self-organs from autologous stem cells and transplant them as syngrafts back into donor patients. To this end, we have striven to establish an in vitro organ factory to build up complex organ structures from autologous adult stem cells by using the kidney as a target organ. Cultivation of human mesenchymal stem cells in growing rodent embryos enables their differentiation within a spatially and temporally appropriate developmental milieu, facilitating the first step of nephrogenesis. We show that a combination of whole-embryo culture, followed by organ culture, encourages exogenous human mesenchymal stem cells to differentiate and contribute to functional complex structures of the new kidney.
Yokoo
Takashi
T
Department of Internal Medicine and Gene Therapy and Pediatrics, Institute of DNA Medicine, Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan. tyokoo@jikei.ac.jp
Ohashi
Toya
T
Shen
Jin Song
JS
Sakurai
Ken
K
Miyazaki
Yoichi
Y
Utsunomiya
Yasunori
Y
Takahashi
Masanori
M
Terada
Yoshio
Y
Eto
Yoshikatsu
Y
Kawamura
Tetsuya
T
Osumi
Noriko
N
Hosoya
Tatsuo
T
D000818:Animals; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008648:Mesoderm / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*
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