title: Human mesenchymal stem cells in rodent whole-embryo culture are reprogrammed to contribute to kidney tissues.
abstract: The use of stem cells has enabled the successful generation of simple organs. However, anatomically complicated organs such as the kidney have proven more refractory to stem-cell-based regenerative techniques. Given the limits of allogenic organ transplantation, an ultimate therapeutic solution is to establish self-organs from autologous stem cells and transplant them as syngrafts back into donor patients. To this end, we have striven to establish an in vitro organ factory to build up complex organ structures from autologous adult stem cells by using the kidney as a target organ. Cultivation of human mesenchymal stem cells in growing rodent embryos enables their differentiation within a spatially and temporally appropriate developmental milieu, facilitating the first step of nephrogenesis. We show that a combination of whole-embryo culture, followed by organ culture, encourages exogenous human mesenchymal stem cells to differentiate and contribute to functional complex structures of the new kidney.
authors.affiliation: Department of Internal Medicine and Gene Therapy and Pediatrics, Institute of DNA Medicine, Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan. tyokoo@jikei.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoo
authors.forename: Toya
authors.initials: T
authors.lastname: Ohashi
authors.forename: Jin Song
authors.initials: JS
authors.lastname: Shen
authors.forename: Ken
authors.initials: K
authors.lastname: Sakurai
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Utsunomiya
authors.forename: Masanori
authors.initials: M
authors.lastname: Takahashi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Terada
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Eto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kawamura
authors.forename: Noriko
authors.initials: N
authors.lastname: Osumi
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Hosoya
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008648:Mesoderm / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*
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