Arabidopsis ADC genes involved in polyamine biosynthesis are essential for seed development.
Arginine decarboxylase (ADC) is a rate-limiting enzyme that catalyzes the first step of polyamine (PA) biosynthesis in Arabidopsis thaliana. We generated a double mutant deficient in Arabidopsis two ADC genes (ADC1-/- ADC2-/-) and examined their roles in seed development. None of the F2 seedlings from crosses of adc1-1 and adc2-2 had the ADC1-/- ADC2-/- genotype. In addition, some abnormal seeds were observed among the ADC1+/- ADC2-/- and ADC1-/- ADC2+/- siliques. Viable offspring with the ADC1-/- ADC2-/- genotype could not be obtained from the ADC1+/- ADC2-/- and ADC1-/- ADC2+/- plants. These results indicate that AtADC genes are required for production of polyamines that are essential for normal seed development in Arabidopsis.
Urano
Kaoru
K
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 350-0074, Japan.
Hobo
Tokunori
T
Shinozaki
Kazuo
K
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D002262:Carboxy-Lyases / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D009154:Mutation / Q000235:genetics; D011073:Polyamines / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D013997:Time Factors
D004269:DNA, Bacterial; D011073:Polyamines; C036483:T-DNA; D002262:Carboxy-Lyases; C019722:arginine decarboxylase