Capillary electrophoresis direct enantioseparation of aromatic amino acids based on mixed chelate-inclusion complexation of aminoethylamino-beta-cyclodextrin.
6(A)-(2-Aminoethylamino)-6(A)-deoxy-beta-cyclodextrin (CDen) was synthesized and formed a binary complex with Cu(II) which was shown to be an effective chiral selector for separation of underivatized amino acid enantiomers in capillary electrophoresis (CE). Moreover, the chiral resolution was greatly enhanced by the presence of polyethyl glycol (PEG) and tert-butyl alcohol in the running buffer. The optimum experimental conditions were 20 mmol/L CDen, 20 mmol/L CuSO(4).5H(2)O, 5.0 mg/mL PEG20000 and 1.0% v/v tert-butyl alcohol, pH 5.80. With the proposed method, the four selected aromatic chiral amino acid pairs were separated in less than 15 min.
Wu
Bidong
B
Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Wang
Qingqing
Q
Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Liu
Qin
Q
Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Xie
Jianwei
J
Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Yun
Liuhong
L
Beijing Institute of Pharmacology and Toxicology, Beijing, China.
D024322:Amino Acids, Aromatic / Q000302:isolation & purification*; D002021:Buffers; D003300:Copper / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*; D020002:tert-Butyl Alcohol
C502270:6(A)-(2-aminoethylamino)-6(A)-deoxy-beta-cyclodextrin; D024322:Amino Acids, Aromatic; D002021:Buffers; D047392:beta-Cyclodextrins; D011092:Polyethylene Glycols; D003300:Copper; D020002:tert-Butyl Alcohol
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