High-performance liquid chromatographic determination of sumatriptan after in vitro transdermal diffusion studies.
A simple, accurate, precise and rapid HPLC method with UV detection has been validated in order to determine the in vitro transdermal absorption of sumatriptan succinate. The HPLC method is a modification of that described by Nozal et al. [M.J. Nozal, J.L. Bernal, L. Toribio, M.T. Martin, F.J. Diez, J. Pharm. Biomed. Anal. 30 (2002) 285-291]. Separation was carried out on a 250 mm Kromasil C18 column at room temperature. The detector response, at 282.7 nm, was found to be linear in a concentration range between 0.145 and 145 microM. The limit of detection (LOD) was 0.019 microM and the limit of quantification (LOQ) was 0.145 microM.
Femenía-Font
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Departamento de Fisiología, Farmacología y Toxicología, Facultad de Ciencias Experimentales y de la Salud, Universidad Cardenal Herrera-CEU, 46113 Moncada, Spain.
Merino
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Rodilla
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López-Castellano
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D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015194:Diffusion Chambers, Culture / Q000379:methods; D066298:In Vitro Techniques; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption; D018170:Sumatriptan / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D013552:Swine
D018170:Sumatriptan