Loss of heterozygosity on chromosome 2 in Japanese patients with prostate cancer.
BACKGROUND
Loss of heterozygosity (LOH) on chromosome 2 is thought to occur only occasionally in prostate cancer (PCa), but allelic losses in this region are frequent in other types of human cancer, such as lung, thyroid, head and neck, and cervix. Here, we show a high-resolution deletion map of markers on chromosome 2 in Japanese patients with PCa.


METHODS
Tissue samples were obtained from 66 patients with PCa. DNA from normal, tumor, or metastatic tissue was used as the template for polymerase chain reaction amplification for LOH using 24 microsatellite markers on human chromosome 2.


RESULTS
Nineteen of the 66 cases (29%) showed LOH for at least one locus on chromosome 2. LOH on 2p was observed more frequently in cancer death cases than in organ confined and regional diseases (P < 0.001). Paired DNAs were available from both primary and metastatic tumors in the eight cases of cancer death; among those pairs, we detected LOH on 2p in four primary tumors, and in all metastatic foci (P < 0.05). Detailed deletion mapping in these tumors identified four distinct commonly deleted regions on 2p 16.3, 2p 12-cent, 2q 21.3, and 2q 23.1-2q 32.1.


CONCLUSIONS
These results suggest that inactivation of putative tumor suppressor genes on chromosome 2 that may play an important role in the progression of Japanese patients with PCa.
Ueda
Takeshi
T
Department of Urology, Graduate School of Medicine Chiba University, Chuo-ku, Chiba-shi, Chiba, Japan. ueda3713@yahoo.co.jp
Komiya
Akira
A
Suzuki
Hiroyoshi
H
Shimbo
Masaki
M
Sakamoto
Shinichi
S
Imamoto
Takashi
T
Akakura
Koichiro
K
Shiraishi
Taizo
T
Ichikawa
Tomohiko
T
D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D016147:Genes, Tumor Suppressor; D006801:Humans; D007564:Japan; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000556:secondary