title: The signal recognition particle and its interactions during protein targeting.
abstract: The synthesis of secretory or integral membrane proteins can be directly coupled to their translocation across or insertion into membranes. In co-translational targeting, the translation machine, the ribosome, is transferred to the respective membrane by the signal recognition particle (SRP) and its receptor (SR) as soon as a signal sequence emerges. Protein synthesis can continue at the membrane, with the nascent peptide chain directly inserting into the ribosome-bound protein-conducting channel, the Sec61 complex. During the past two years, several structures have been solved by crystallography and cryo-electron microscopy that represent distinct functional states of the SRP cycle. On this basis, the first structure-based models can be suggested that explain important aspects of protein targeting, such as the SRP-ribosome and SRP-SR interactions.
authors.affiliation: Institut für Biochemie, Charité, Universitätsmedizin Berlin, Monbijoustrasse 2, D-10117 Berlin, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Halic
authors.forename: Roland
authors.initials: R
authors.lastname: Beckmann
mesh_terms: D001665:Binding Sites; D002462:Cell Membrane / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D021382:Protein Sorting Signals; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry*; D018000:Receptors, Peptide / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry*; D018271:Signal Recognition Particle / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D021382:Protein Sorting Signals; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D018271:Signal Recognition Particle; C036318:signal peptide receptor