title: Effects of ICI 182780 on estrogen receptor expression, fluid absorption and sperm motility in the epididymis of the bonnet monkey.
abstract: BACKGROUND
  The importance of estrogen in regulation of fluid absorption and sperm maturation in the rodent epididymis has been established from studies on estrogen receptor-alpha knockout mice. However, functional studies on the role of estrogen in primate epididymis have been few. The main objective of this study was therefore to extend these observations and systematically analyze the presence and function of estrogen receptors in modulating the function of the primate epididymis, using the bonnet monkey (Macaca radiata) as a model system.
  
  
  METHODS
  A steroidal estrogen receptor (ER) antagonist, ICI 182780 (ICI), was administered to adult male bonnet monkeys via mini-osmotic pumps for a duration of 30 to 180 days. The expression of key estrogen-regulated genes (ER-alpha, Na-K ATPase alpha-1 and Aquaporin-1) was examined at specific time points. Further, the effect of ICI in modulating fluid reabsorption in efferent ductules was monitored, and critical sperm-maturation parameters were also analyzed.
  
  
  RESULTS
  Our studies in the bonnet monkey revealed that both ER-alpha and ER-beta were expressed in all the three regions of the epididymis. We observed an increase in ER-alpha mRNA and protein in the caput of ICI-treated monkeys. Steady state mRNA levels of the water-channel protein, Aquaporin-1, was significantly lower in the caput of ICI-treated monkeys compared to controls, whereas the mRNA levels of Na-K ATPase alpha-1 remained unchanged. In vitro incubation of efferent ductules with ICI resulted in two-fold increase in tubular diameter, indicating affected fluid reabsorption capacity. Furthermore, sperm from ICI-treated monkeys were immotile.
  
  
  CONCLUSIONS
  Taken together, our results point to an integral role for estrogen in modulating the functions of the bonnet monkey epididymis. This study also demonstrates possible differences in the epididymal physiology of rodents and non-human primates, and thus underscores the significance of reports such as these, that examine the physiology of non-human primates (as opposed to rodents), in an attempt to understand similar events in the human.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore- 560012, India. shayu@biochem.iisc.ernet.in <shayu@biochem.iisc.ernet.in>
authors.forename: Deshpande
authors.initials: D
authors.lastname: Shayu
authors.forename: C S
authors.initials: CS
authors.lastname: ChennaKesava
authors.forename: Rama
authors.initials: R
authors.lastname: Soundarajan
authors.forename: A Jagannadha
authors.initials: AJ
authors.lastname: Rao
mesh_terms: D000042:Absorption / Q000502:physiology; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism; D004822:Epididymis / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000032:analysis / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000032:analysis / Q000378:metabolism*; D004967:Estrogens / Q000502:physiology*; D000077267:Fulvestrant; D008255:Macaca radiata; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013076:Sperm Count; D013079:Sperm Maturation / Q000187:drug effects / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects*; D013739:Testosterone / Q000097:blood
chemical_list: D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D012333:RNA, Messenger; D051398:Aquaporin 1; D000077267:Fulvestrant; D013739:Testosterone; D004958:Estradiol; D000254:Sodium-Potassium-Exchanging ATPase
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