Embryonic disc development and subsequent viability of cattle embryos following culture in two media under two oxygen concentrations.
Bovine embryos were produced in vitro using a 2 x 2 design of modified medium (KSOM or SOF) and oxygen concentration (5% or 20%). Day 7 blastocysts were transferred in bulk (n = 11, on average) to recipient heifers and recovered non-surgically at Day 14. In two replications of a Latin square, eight heifers received embryos from each combination of factors. Recovered embryos were evaluated for trophoblast length and width, as well as the presence and diameter of an embryonic disc (ED). An ED was detected in a higher percentage of embryos that had been cultured in KSOM than SOF (72% v. 46%, respectively; P < 0.05). The aim of a second series of experiments was to associate Day 14 morphology with subsequent developmental capacity. In vitro-produced blastocysts were transferred (n = 17-20) on Day 7 to each of eight heifers and recovered at Day 14. Thirty-eight blastocysts were retransferred to heifers following morphological evaluation. Embryos in which an ED with no signs of degeneration had been detected maintained more pregnancies than other embryos in which an ED had either shown signs of degeneration or had not been detected (5/8 v. 2/30, respectively; P < 0.01). Further investigation into ED integrity at the elongating stage may contribute to our understanding of pregnancy establishment and maintenance.
Fischer-Brown
A E
AE
Department of Animal Sciences, University of Wisconsin, Madison, WI 53706, USA. afischer@ansci.wisc.edu
Lindsey
B R
BR
Ireland
F A
FA
Northey
D L
DL
Monson
R L
RL
Clark
S G
SG
Wheeler
M B
MB
Kesler
D J
DJ
Lane
S J
SJ
Weigel
K A
KA
Rutledge
J J
JJ
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D002470:Cell Survival; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000502:physiology; D047108:Embryonic Development; D005260:Female; D010100:Oxygen / Q000032:analysis / Q000494:pharmacology*; D011247:Pregnancy
D003470:Culture Media; D010100:Oxygen