title: Short report: neonatal screening pilot study of Toxoplasma gondii congenital infection in Mexico.
abstract: Congenital toxoplasmosis is an obstetric problem in Mexico, but its actual frequency is unknown. Using a network for screening of non-infectious disorders, we performed a pilot study to determine the frequency of IgM antibodies to Toxoplasma gondii in 1,003 infants (53.1% male, mean +/- SD age = 18.3 +/- 13.0 days, birth weight = 3.116 +/- 0.453 kg) in Mexico City from March to April 2003. Blood samples embedded in filter paper were assayed for IgM antibodies using a capture enzyme-linked immunosorbent assay and results were confirmed by Western blot. Two asymptomatic newborns, one of them premature, had IgM and IgG antibodies in a serum sample taken from both the infant and the mother and were clinically followed. Our data suggest a frequency of approximately two cases of congenital T. gondii infection per 1,000 newborns in Mexico City.
authors.affiliation: Servicio de Genética de la Nutrición, Instituto Nacional de Pediatría, Secretaría de Salud, Mexico City, Mexico. amieva@servidor.unam.mx <amieva@servidor.unam.mx>
authors.forename: Marcela
authors.initials: M
authors.lastname: Vela-Amieva
authors.forename: Irma
authors.initials: I
authors.lastname: Cañedo-Solares
authors.forename: Pedro
authors.initials: P
authors.lastname: Gutiérrez-Castrellón
authors.forename: Martha
authors.initials: M
authors.lastname: Pérez-Andrade
authors.forename: Claudia
authors.initials: C
authors.lastname: González-Contreras
authors.forename: Joel
authors.initials: J
authors.lastname: Ortíz-Cortés
authors.forename: Venancio
authors.initials: V
authors.lastname: Ortega-Velázquez
authors.forename: Maria de la Luz
authors.initials: Mde L
authors.lastname: Galván-Ramírez
authors.forename: Matilde
authors.initials: M
authors.lastname: Ruiz-García
authors.forename: Patrica
authors.initials: P
authors.lastname: Saltigeral-Simentel
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Ordaz-Favila
authors.forename: Carmen
authors.initials: C
authors.lastname: Sánchez
authors.forename: Dolores
authors.initials: D
authors.lastname: Correa
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control*; D008297:Male; D008800:Mexico / Q000453:epidemiology; D010865:Pilot Projects; D015995:Prevalence; D012307:Risk Factors; D014122:Toxoplasma / Q000276:immunology; D014125:Toxoplasmosis, Congenital / Q000453:epidemiology* / Q000209:etiology / Q000517:prevention & control* / Q000635:transmission
chemical_list: D000913:Antibodies, Protozoan