The prevalence of bacterial resistance in clinical, food, water and some environmental samples in Southwest Nigeria.
The resistance pattern and mechanisms of bacterial isolates obtained from clinical origin, soil, industrial effluent, orange juice products and drinking water were studied using commonly used antibiotics. The microbial load of the water samples, industrial effluent and orange juice products were 1.0 x 10(1)-2.25 x 10(6), 2.15 x 10(5), and 3.5 x 10(4)-2.15 x 10(5) cfu mL(-1), respectively. The faecal coliform test revealed that only two out of twenty orange juice products had MPN of 2 and 20, the MPN of water ranged from 1-> or = 1800, while the effluent had MPN of > or = 1800. The bacterial isolates that were isolated include E. coli, S. aureus, P. vulgaris, S. marcescens, S. pyogenes, B. cereus, B. subtilis, Micrococcus sp., Klebsiella sp., P. aeruginosa, and Enterobacter sp. Also, clinical and soil isolates of P. aeruginosa were used in the study. Among the eight antibiotics tested for resistance on five strains of each bacterium, seven different resistance patterns were observed among the bacterial isolates obtained from water, effluent and orange juice products. Among the clinical and soil isolates of P. aeruginosa, four multiple-drug resistance patterns were obtained. Thirty strains of E. coli and S. aureus were tested for beta-lactamase production and fourteen strains, seven each of E. coli and S. aureus that had high Minimum Inhibitory Concentration values (MIC) for both Amoxycillin and Cloxacillin were positive.
Lateef
A
A
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria. agbaje72@yahoo.com
Oloke
J K
JK
Gueguimkana
E B
EB
D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D001628:Beverages; D002957:Citrus / Q000737:chemistry*; D003023:Cloxacillin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects; D005516:Food Microbiology; D008826:Microbial Sensitivity Tests; D009549:Nigeria; D013211:Staphylococcus aureus / Q000187:drug effects; D014871:Water Microbiology; D001618:beta-Lactamases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000658:Amoxicillin; D001618:beta-Lactamases; D003023:Cloxacillin
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