The genome of the Pseudomonas aeruginosa generalized transducing bacteriophage F116.
F116 is a temperate, pilus-specific, generalized transducing phage belonging to the Podoviridae virus family. Its genome is linear, ds, TR, and CP DNA with a GC content of 63.2%. The 65 195-bp genome contains 70 putative ORFs, only 16 of which showed sequence similarity to Pseudomonas genomic or phage genes. While the current literature suggests that F116 is a non-integrating phage that maintains itself as a plasmid during the lysogenic life cycle, a putative int gene was identified. Of the phage structural genes, only the portal protein could be identified by homology. Analysis of F116 structural protein by one-dimensional SDS-PAGE revealed approximately 15 bands. MALDI-TOF MS analysis identified the gene encoding the major capsid protein. This protein appears to undergo posttranslational cleavage giving rise to a smaller capsid protein.
Byrne
Michaela
M
Queen's University, Department of Microbiology and Immunology, Kingston, Ontario, Canada K7L 3N6.
Kropinski
Andrew M
AM
D001435:Bacteriophages / Q000235:genetics*; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011995:Recombination, Genetic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization