title: Surface modification of poly(L: -lactic acid) affects initial cell attachment, cell morphology, and cell growth.
abstract: The object of this study was to develop a highly porous scaffold to be used in regeneration of blood vessels, nerves, and other hollow tissues with small openings. Using the phase-inversion method and a mixture of water and methanol as a coagulating agent, we prepared highly porous flat membranes from poly(L: -lactic acid) (PLLA) with numerous pores both on the surface and in the interior of the membranes. Chinese hamster ovary (CHO) cells were cultured on the membranes to evaluate initial cell adhesion, cell proliferation, and cell morphology. Adhesion of CHO cells to PLLA was poor: the cells adhered at approximately half the rate observed with a tissue culture polystyrene dish (TCPS). In contrast, adhesion of cells to PLLA treated with a low-temperature oxygen plasma was good; the adhesion rate was the same as that on TCPS. The rate of cell proliferation on the treated membranes was no different from that on the nontreated membranes, but cell morphologies were quite different. The cells on the nontreated membranes were small and round and proliferated separately from one another. In contrast, the cells on the plasma-treated membranes proliferated in close contact with other cells, spreading out extensively in sheet-like formations. Since the plasma treatment not only accelerated cell adhesion but also enabled cells to proliferate in the form of sheets resembling biological tissue, we believe that oxygen-plasma treatment is extremely effective for modifying surfaces of materials used for tissue regeneration.
authors.affiliation: Bio-Nanomaterials Team, Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Manae
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Toshio
authors.initials: T
authors.lastname: Shinbo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori
authors.forename: Pi-chao
authors.initials: PC
authors.lastname: Wang
authors.forename: Motohiro
authors.initials: M
authors.lastname: Niwa
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Kawakami
authors.forename: Shoji
authors.initials: S
authors.lastname: Nagaoka
authors.forename: Kohichiro
authors.initials: K
authors.lastname: Hirakawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kamiya
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects*; D002462:Cell Membrane / Q000648:ultrastructure; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D005260:Female; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D023421:Models, Animal; D010053:Ovary / Q000166:cytology*; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D016062:Porosity; D011336:Probability; D012680:Sensitivity and Specificity; D013499:Surface Properties / Q000187:drug effects; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)