title: Menaquinone-specific prenyl reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus.
abstract: Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a distinct change in the quinone profile of the host cells, although the homologue is the most distantly related to the geranylgeranyl reductase. The new compounds found in the profile had successively longer elution times than those of ordinary quinones from E. coli, i.e., menaquinone-8 and ubiquinone-8, in high-performance liquid chromatography on a reversed-phase column. Structural analyses of the new compounds by electron impact-mass spectrometry indicated that their molecular masses progressively increase relative to the ordinary quinones at a rate of 2 U but that they still contain quinone head structures, strongly suggesting that the compounds are quinones with partially saturated prenyl side chains. In vitro assays with dithionite as the reducing agent showed that the prenyl reductase is highly specific for menaquinone-7, rather than ubiquinone-8 and prenyl diphosphates. This novel enzyme noncovalently binds flavin adenine dinucleotide, similar to geranylgeranyl reductase, but was not able to utilize NAD(P)H as the electron donor, unlike the plant homologue.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan. hhemmi@seika.che.tohoku.ac.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hemmi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Kyohei
authors.initials: K
authors.lastname: Shibuya
authors.forename: Toru
authors.initials: T
authors.lastname: Nakayama
authors.forename: Tokuzo
authors.initials: T
authors.lastname: Nishino
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019611:Archaeoglobus fulgidus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004227:Dithionite / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism; D024482:Vitamin K 2 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D013729:Terpenes; D024482:Vitamin K 2; D004227:Dithionite; D010088:Oxidoreductases; C110920:geranylgeranyl reductase; D010100:Oxygen
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