title: A new polymorphism in the type II deiodinase gene is associated with circulating thyroid hormone parameters.
abstract: Type II deiodinase (D2) is important in the regulation of local thyroid hormone bioactivity in certain tissues. D2 in skeletal muscle may also play a role in serum triiodothyronine (T(3)) production. In this study, we identified a polymorphism in the 5'-UTR of the D2 gene (D2-ORFa-Gly3Asp). We investigated the association of D2-ORFa-Gly3Asp, and of the previously identified D2-Thr92Ala polymorphism, with serum iodothyronine levels. D2-ORFa-Gly3Asp was identified by sequencing the 5'-UTR of 15 randomly selected individuals. Genotypes for D2-ORFa-Gly3Asp were determined in 156 healthy blood donors (age 46.3 +/- 12.2 yr) and 349 ambulant elderly men (age 77.7 +/- 3.5 yr) and related to serum iodothyronine and TSH levels. D2-ORFa-Asp(3) had an allele frequency of 33.9% in blood bank donors and was associated with serum thyroxine (T(4); Gly/Gly vs. Gly/Asp vs. Asp/Asp = 7.06 +/- 0.14 vs. 6.74 +/- 0.15 vs. 6.29 +/- 0.27 microg/dl, P = 0.01), free T(4) (1.22 +/- 0.02 vs. 1.16 +/- 0.02 vs. 1.06 +/- 0.04 ng/dl, P = 0.001), reverse T(3) (P = 0.01), and T(3)/T(4) ratio (P = 0.002) in a dose-dependent manner, but not with serum T(3) (P = 0.59). In elderly men, D2-ORFa-Asp(3) had a similar frequency but was not associated with serum iodothyronine levels. This new polymorphism in the 5'-UTR of D2 is associated with iodothyronine levels in blood donors but not in elderly men. We hypothesize that this might be explained by the decline in skeletal muscle size during aging, resulting in a relative decrease in the contribution of D2 to serum T(3) production.
authors.affiliation: Department of Internal Medicine, Rm. Ee 502, Erasmus University Medical Center, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. r.peeters@erasmusmc.nl
authors.forename: Robin P
authors.initials: RP
authors.lastname: Peeters
authors.forename: Annewieke W
authors.initials: AW
authors.lastname: van den Beld
authors.forename: Hayat
authors.initials: H
authors.lastname: Attalki
authors.forename: Hans van
authors.initials: Hv
authors.lastname: Toor
authors.forename: Yolanda B
authors.initials: YB
authors.lastname: de Rijke
authors.forename: George G J M
authors.initials: GG
authors.lastname: Kuiper
authors.forename: Steven W J
authors.initials: SW
authors.lastname: Lamberts
authors.forename: Joop A M J L
authors.initials: JA
authors.lastname: Janssen
authors.forename: Andre G
authors.initials: AG
authors.lastname: Uitterlinden
authors.forename: Theo J
authors.initials: TJ
authors.lastname: Visser
mesh_terms: D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics / Q000378:metabolism; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D015994:Incidence; D007453:Iodide Peroxidase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009426:Netherlands / Q000453:epidemiology; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D013223:Statistics as Topic; D013963:Thyroid Hormones / Q000097:blood*; D000097564:Iodothyronine Deiodinase Type II
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; D007453:Iodide Peroxidase; D013963:Thyroid Hormones; D000097564:Iodothyronine Deiodinase Type II; C414206:CYP2A13 protein, human