Ecdysis of Oesophagostomum: possible involvement of eicosanoids and development of a bioassay.
Bioassays were developed and applied to test the role of eicosanoids and pH changes in ecdysis of Oesophagostomum dentatum. Exsheathment (80-100%) was achieved by subjecting third-stage larvae (L3) either to chlorine (hypochlorite assay) for 5 min or by incubating them in HCl followed by addition of NaHCO3 (pH-change assay) with subsequent cultivation at 38.5 degrees C/10% CO2 for 1 week. Addition of the lipoxygenase (LOX) inhibitor diethylcarbamacine (DEC) to the larvae resulted in a reduction of the exsheathment rates which could be restored by the addition of leukotrienes (LT)B4, LTC4, LTD4 and LTE4. Addition of the cyclooxygenase (COX) inhibitor acetylsalicylic acid (ASA) also resulted in decreased exsheathment rates both in the hypochlorite and in the pH-change assays in a dose-dependent manner. However, the primary COX products (prostaglandins) were not able to reverse this effect, in contrast to LTC4. It was concluded that: (1) both tests are suitable for bioassaying the effect of substances on exsheathment, and (2) eicosanoids involved in the control of exsheathment of L3 of O. dentatum are primarily LT.
Joachim
A
A
Institute of Parasitology and Zoology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria. Anja.Joachim@vu-wien.ac.at
Ruttkowski
B
B
Daugschies
A
A
D000818:Animals; D001681:Biological Assay; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015777:Eicosanoids / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D015289:Leukotrienes / Q000378:metabolism / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D018978:Molting / Q000187:drug effects* / Q000502:physiology; D009814:Oesophagostomiasis / Q000469:parasitology / Q000662:veterinary; D009815:Oesophagostomum / Q000187:drug effects / Q000254:growth & development*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology
D016861:Cyclooxygenase Inhibitors; D015777:Eicosanoids; D015289:Leukotrienes; D016859:Lipoxygenase Inhibitors; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases
Mol Biochem Parasitol. 2002 Apr 9;120(2):291-6
11897134
J Parasitol. 1996 Feb;82(1):100-2
8627476
Mol Biochem Parasitol. 1989 Feb;33(1):49-58
2651914
Int J Parasitol. 1976 Aug;6(4):315-9
821874
Adv Parasitol. 2000;46:181-240
10761556
J Parasitol. 1970 Feb;56(1):138-43
5413837
Parasitology. 2002 Jul;125(Pt 1):65-70
12166522
Exp Parasitol. 1964 Aug;15:314-24
14201362
Z Parasitenkd. 1967;29(3):242-74
5603180
J Parasitol. 1973 Jun;59(3):417-24
4123105
Int J Parasitol. 1982 Dec;12(6):495-502
6298132
Parasitol Res. 1997;83(6):563-8
9211507
Int J Parasitol. 1998 Feb;28(2):355-62
9513001
Exp Parasitol. 1995 Dec;81(4):574-83
8542999
Parasitol Res. 1996;82(5):416-22
8738280
J Parasitol. 1997 Oct;83(5):971-3
9379314