Coexpression and regulation of the FGF-2 and FGF antisense genes in leukemic cells.
Fibroblast growth factor-2 (FGF-2) is a growth and survival factor whose expression is elevated in many hematopoietic malignancies. A natural antisense RNA (FGF-AS) has been implicated in the posttranscriptional regulation of FGF-2 mRNA expression. We demonstrate for the first time that FGF sense and antisense RNAs are coordinately expressed and translated in hematopoietic cells and tissues. Cytokine stimulation of growth-arrested K562 cells elicited a rapid transient increase in FGF-AS mRNA expression followed by a slower but sustained increase in FGF-2 mRNA. This was accompanied by a marked increase in the expression and nuclear translocation of FGF-2 and the FGF-AS encoded protein, GFG/NUDT6. These findings suggest a role for both FGF-2 and GFG proteins in the cell survival and proliferation of lymphoid and myeloid tumor cells.
Baguma-Nibasheka
Mark
M
Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, 5850 College Street, Halifax, Nova Scotia, Canada B3H 1X5.
Li
Audrey W
AW
Osman
Mohammed S
MS
Geldenhuys
Laurette
L
Casson
Alan G
AG
Too
Catherine K L
CK
Murphy
Paul R
PR
D017398:Alternative Splicing; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D017931:DNA Primers; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D005346:Fibroblast Growth Factors / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D011388:Prolactin / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D011388:Prolactin