[Selection of TLC solvent system for separating substituted ferrocyclopentadienyl borane using orthogonal array method].
A series of substituted ferro-cyclopentadienyl borane compounds were separated by using thin-layer chromatographic technique. The optimum mobile phase was acetone-cyclohexane-tetraethyl ammonium halide (0.59:8.24:1.18). The orthogonal array method was used to get the optimum chromatographic condition. The types and the concentrations of different kinds of ion-pair reagents, the contents of acetone and cyclohexane were considered as factors which affected the results of separation. The operating conditions for each experiment was adopted based on the L9 (3(4)) design. A statistical method of variance analysis was used to evaluate the overall degree of separation (D(S)), a criterion which reflects the degree of optimization. As a result, we found the most important factors that affected the separation and the optimum chromatographic condition for 8 compounds of this sort.
Yang
X
X
Chemistry Department of Nankai University, Tianjin, 300071.
Wu
L
L
Pei
Y
Y
Hu
P
P