[Study of collections of Mycobacterium non-tuberculosis by a restriction test of the amplified fragment spacer sequence 16S-23S of ribosomal DNA].
Fifty mycobacterial cultures were studied by restriction analysis of the amplified fragment of spacer sequence 16S-23S of ribosomal DNA. The following types were identified: M. tuberculosis complex, M. avium, M. intracellulare, M. scrofulaceum, M. kansasii, M. gordonae, M. ulcerans, M. fortuitum, M. smegmatis. The genetic heterogeneity of M. fortuitum was detected. The strains under study belong to 5 patterns: M. fortuitum I, M. fortuitum II, M. fortuitum X, M. fortuitum Y, M. fortuitum Z. The pattern characteristics for M. ulcerans (the size of a CPR product was 370 pn; the characteristics of the enzyme HaeIII-induced restriction profile was 214/155 pn) were obtained.
Maĭorova
A A
AA
Stepanshina
V N
VN
Shemiakin
I G
IG
Korobova
O V
OV
D017931:DNA Primers; D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases