[Spectroscopic analysis of the interaction of ethanol and acid phosphatase from wheat germ].
Conformational and activity changes of acid phosphatase from wheat germ in ethanol solutions of different concentrations were measured by fluorescence spectra and differential UV-absorption spectra. The effect of ethanol on kinetics of acid phosphatase was determined by using the double reciprocal plot. The results indicate the ethanol has a significant effect on the activity and conformation of acid phosphatase. The activity of acid phosphatase decreased linearly with increasing the concentration of ethanol. Differential UV-absorption spectra of the enzyme denatured in ethanol solutions showed two positive peaks at 213 and 234 nm, respectively. The peaks on the differential UV-absorption spectra suggested that the conformation of enzyme molecule changed from orderly structure to out-of-order crispation. The fluorescence emission peak intensity of the enzyme gradually strengthened with increasing ethanol concentration, which is in concordance with the conformational change of the microenvironments of tyrosine and tryptophan residues. The results indicate that the expression of the enzyme activity correlates with the stability and integrity of the enzyme conformation to a great degree. Ethanol is uncompetitive inhibitor of acid phosphatase.
Xu
Dong-mei
DM
Environmental Science Institute of Zhejiang University, Hangzhou 310029, China.
Liu
Guang-shen
GS
Wang
Li-ming
LM
Liu
Wei-ping
WP
D000135:Acid Phosphatase / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013057:Spectrum Analysis; D014908:Triticum / Q000737:chemistry* / Q000201:enzymology
D010938:Plant Oils; C050055:wheat germ oil; D000431:Ethanol; D000135:Acid Phosphatase