The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice.
We have investigated to develop novel vaccines against SARS CoV using cDNA constructs encoding the structural antigen; spike protein (S), membrane protein (M), envelope protein (E), or nucleocapsid (N) protein, derived from SARS CoV. Mice vaccinated with SARS-N or -M DNA using pcDNA 3.1(+) plasmid vector showed T cell immune responses (CTL induction and proliferation) against N or M protein, respectively. CTL responses were also detected to SARS DNA-transfected type II alveolar epithelial cells (T7 cell clone), which are thought to be initial target cells for SARS virus infection in human. To determine whether these DNA vaccines could induce T cell immune responses in humans as well as in mice, SCID-PBL/hu mice was immunized with these DNA vaccines. As expected, virus-specific CTL responses and T cell proliferation were induced from human T cells. SARS-N and SARS-M DNA vaccines and SCID-PBL/hu mouse model will be important in the development of protective vaccines.
Okada
Masaji
M
Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone, Sakai, Osaka 591-8555, Japan. okm@kch.hosp.go.jp
Takemoto
Yuji
Y
Okuno
Yoshinobu
Y
Hashimoto
Satomi
S
Yoshida
Shigeto
S
Fukunaga
Yukari
Y
Tanaka
Takao
T
Kita
Yoko
Y
Kuwayama
Sachiko
S
Muraki
Yumiko
Y
Kanamaru
Noriko
N
Takai
Hiroko
H
Okada
Chika
C
Sakaguchi
Yayoi
Y
Furukawa
Izumi
I
Yamada
Kyoko
K
Matsumoto
Makoto
M
Kase
Tetsuo
T
Demello
Daphne E
DE
Peiris
J S M
JS
Chen
Pei-Jer
PJ
Yamamoto
Naoki
N
Yoshinaka
Yoshiyuki
Y
Nomura
Tatsuji
T
Ishida
Isao
I
Morikawa
Shigeru
S
Tashiro
Masato
M
Sakatani
Mitsunori
M
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014183:Transplantation, Heterologous; D019444:Vaccines, DNA / Q000235:genetics / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines