[Analyzing genetic quality of BALB/c mouse strains in China by microsatellite marking].
Eleven BALB/c mouse strains of Beijing, Shanghai, Shenyang, Haerbin, Guangzhou, Chongqing and Changchun were monitored in order to assure the genetic quality of inbred BALB/c mouse strains in China and to estimate the credibility of microsatllite markers. Fourteen microsatellites loci on different chromosomes were investigated by PCR analysis. It showed that all these microsatellites DNA loci displayed single allelic gene band in mouse strains of Beijing, Shanghai and Haerbin. But the mice came from Shenyang, Guangzhou, Chongqing and Changchun had polymorphisms or heterozygosis, among which the Shenyang and Changchun strains showed polymorphisms and heterozygosis at two separate loci. Four loci showed polymorphisms or heterozygosis in one of the Guangzhou mouse strains. The Chongqing strains showed polymorphisms and heterozygosis at seven loci, including the D10Mit180 locus as compared with the Shanghai strains.
Chen
Zhen-Wen
ZW
Department of Laboratory Animal Science, Capital University of Medical Sciences, Beijing, China. czwen@sohu.com
Ouyang
Zhao-He
ZH
Dong
Gang
G
Li
Rui-Sheng
RS
D000483:Alleles; D000818:Animals; D002681:China; D005260:Female; D005828:Genetics, Population / Q000379:methods / Q000592:standards; D006579:Heterozygote; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011786:Quality Control; D015203:Reproducibility of Results