title: Structural and genetic characterization of the Shigella boydii type 10 and type 6 O antigens.
abstract: Comparison of the O antigens of Shigella boydii types 10 and 6 by chemical analysis and nuclear magnetic resonance spectroscopy showed that their structures are similar, with the only difference being the presence or absence of d-ribofuranose, which is the immunodominant sugar in S. boydii type 10. In S. boydii type 6, a residue previously reported as alpha-d-GlcpA, was shown to be beta-d-GlcpA as in S. boydii type 10. S. boydii types 10 and 6 are reported not to cross-react serologically, and the role of d-ribofuranose in the specificity of S. boydii was confirmed by making a mutant of type 10 that lacked d-ribofuranose. However, S. boydii type 11, which has a d-ribofuranose but with different linkage does show cross-reaction with type 10. The O-antigen gene loci of S. boydii types 10 and 6 were shown to be virtually identical except that orf8 (wbaM), which was confirmed as the ribofuranosyltransferase gene, is interrupted by IS629 in type 6. Therefore, it is proposed that the O-antigen gene cluster of S. boydii type 6 was derived from type 10 by an IS element insertion.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Jiansong
authors.initials: J
authors.lastname: Cheng
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Peter R
authors.initials: PR
authors.lastname: Reeves
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ye
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry / Q000235:genetics*; D012761:Shigella boydii / Q000235:genetics* / Q000276:immunology
chemical_list: D019081:O Antigens