CD4- plasmacytoid dendritic cells (pDCs) migrate in lymph nodes by CpG inoculation and represent a potent functional subset of pDCs.
We have recently identified two groups of plasmacytoid dendritic cells (pDCs) isolated from murine liver based on the expression of CD4 and other cell surface markers uniquely expressed by pDCs. Herein, we describe the identification of both CD4+ and CD4- pDCs that clearly exist in lymph nodes (LNs), spleen, liver, thymus, bone marrow, and lung. Normally, CD4+ pDCs are enriched in LNs. However, after in vivo systemic injection with bacterial CpG, a larger number of CD4- pDCs are recruited to the LNs and local inoculation by CpG drives CD4- pDCs migrating into local sentinel LNs, suggesting that CD4- pDCs are the main subpopulation migrating to the peripheral LNs. Furthermore, although both freshly isolated CD4+ pDCs and CD4- pDCs appear as an immature plasmacytoid cell and develop into a DC morphology following activation, the two subsets have strikingly different immune features, including differences in the production pattern of cytokines stimulated with CpG and in T cell activation.
Yang
Guo-Xiang
GX
Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, CA 95616, USA.
Lian
Zhe-Xiong
ZX
Kikuchi
Kentaro
K
Liu
Yong-Jun
YJ
Ansari
Aftab A
AA
Ikehara
Susumu
S
Gershwin
M Eric
ME
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000145:classification / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D015870:Gene Expression; D066298:In Vitro Techniques; D007519:Isoantigens; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D010950:Plasma Cells / Q000145:classification / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019713:Receptors, CCR5 / Q000235:genetics; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000235:genetics
C498126:CCR6 protein, mouse; D015704:CD4 Antigens; C408982:CPG-oligonucleotide; C515371:Ccr7 protein, mouse; D016207:Cytokines; D007519:Isoantigens; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D019713:Receptors, CCR5; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D019707:Receptors, Chemokine