title: The effect of prolonged cold storage of eland (Taurotragus oryx) cauda epididymides on the spermatozoa: possible implications for the conservation of biodiversity.
abstract: The objectives of this study were to investigate the effects of prolonged storage of cauda epididymides at 4 degrees C on spermatozoa, and to determine the practicality of utilising epididymal sperm, harvested from testes collected during routine culling of game animals, in assisted reproductive technologies. Testes from eland (Taurotragus oryx) were collected and epididymides removed and maintained at 4 degrees C. Sperm motility, viability, morphology and membrane integrity were examined at 12 h intervals for 108 h. Sperm motility and viability were significantly lower at the end of the experiment than at the start (P < 0.05) and there was individual variation in the rate at which motility and viability declined. The total number of normal sperm decreased significantly with prolonged storage at 4 degrees C. Midpiece defects were the most common and head and tail abnormalities were rare. A significant decrease in acrosomal and nuclear membrane integrity was observed with prolonged cold storage but there was no significant change in cell membrane integrity. However, about 30% of epididymal sperm survived for 3 days at 4 degrees C and more than 10% survived for 4 days, and it should be possible to use sperm from culled animals in some assisted reproductive technologies.
authors.affiliation: Department of Zoology and Entomology, Rhodes University, Grahamstown 6139, South Africa. charlene@kwandwe.co.za
authors.forename: C
authors.initials: C
authors.lastname: Bissett
authors.forename: R T F
authors.initials: RT
authors.lastname: Bernard
mesh_terms: D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D044822:Biodiversity; D002462:Cell Membrane / Q000648:ultrastructure; D002470:Cell Survival; D003080:Cold Temperature; D004822:Epididymis / Q000166:cytology / Q000502:physiology*; D008297:Male; D009926:Organ Preservation / Q000662:veterinary*; D012418:Ruminants; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology* / Q000648:ultrastructure