Effect of calcium sulfate-chitosan composite: pellet on bone formation in bone defect.
The purpose of this experiment was to study the effects of chitosan, calcium sulfate, and calcium sulfate-chitosan composite pellet on the osteogenesis of defective tibia in rabbits. Eighty New Zealand white rabbits, each weighing approximately 3 to 3.5 kg, were used for this study. A 1-cm ostectomy was made on the middle of the tibia of each rabbit with the periosteum preserved. Nothing was implanted in the control group (group 1), and five chitosan pellets (60 mg/pellet) were implanted in group 1, three OsteoSet pellets (100 mg/pellet) in group 3, and four calcium sulfate-chitosan composite pellets (1 pellet, 80 mg; calcium sulfate 40 mg/pellet, chitosan 40 mg/pellet) in group 4. For each group, a radiographic study, bone mineral density test, three-point bending test, and histologic examination were performed in the second, fourth, and sixth weeks. In the radiologic study, in group 1, cortical bone was not formed even at 6 weeks. In group 2, it was observed at 6 weeks. In groups 3 and 4, cortical bone was partially seen around the fourth week. At 6 weeks, it was clearly observed on both sides, and the projection of the marrow cavity became distinctive, so bone consolidation was considered to be much progressed. The bone mineral density test and three-point bending test results appeared to be highly similar in groups 3 and 4 and in groups 2 and 1. Particularly at 6 weeks, the measures for groups 3 and 4 were statistically significant compared with those for groups 1 and 2 (P < 0.05). In histologic examination, new bone formation began to be seen at 2 weeks in all groups, but it was more active and faster in groups 3 and 4. At 6 weeks, fibrous connective tissue still remained at the center in groups 1 and 2; however, the fibrous connective tissue at the center was replaced with callus, the bony bridge was obvious, and lamellation of callus was observed more in groups 3 and 4. The results indicate that chitosan pellets, OsteoSet, and chitosan-calcium sulfate composite pellets facilitate new bone formation on defected bone, and that particularly OsteoSet and chitosan-calcium sulfate composite pellets are more effective than chitosan.
Cho
Byung Chae
BC
Departments of Plastic and Reconstructive Surgery, Institute of Cell and Matrix Biology, School of Medicine, Kyungpook National University, Samduk 2 ga 50, 700-721 Daegu, Korea. bccho@knu.ac.kr
Kim
Tae Gyu
TG
Yang
Jung Duk
JD
Chung
Ho Yun
HY
Park
Jae Woo
JW
Kwon
Ick Chan
IC
Roh
Kyung Ho
KH
Chung
Hye Sun
HS
Lee
Dong Sin
DS
Park
Nang Un
NU
Kim
In San
IS
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000627:therapeutic use*; D015519:Bone Density / Q000187:drug effects; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000601:surgery*; D001853:Bone Marrow / Q000187:drug effects; D018786:Bone Substitutes / Q000008:administration & dosage / Q000627:therapeutic use*; D002146:Bony Callus / Q000187:drug effects; D002133:Calcium Sulfate / Q000008:administration & dosage / Q000627:therapeutic use*; D048271:Chitosan / Q000008:administration & dosage / Q000627:therapeutic use*; D003238:Connective Tissue / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D018583:Pliability; D011817:Rabbits; D011859:Radiography; D013314:Stress, Mechanical; D013977:Tibia / Q000000981:diagnostic imaging / Q000601:surgery*; D013997:Time Factors
D001672:Biocompatible Materials; D018786:Bone Substitutes; D048271:Chitosan; D002133:Calcium Sulfate