Gene expression in the salivary complexes from Haementeria depressa leech through the generation of expressed sequence tags.
A survey of the transcriptional profile of Haementeria depressa Ringuelet, 1972 (Annelida, Hirudinea) salivary complexes was produced through expressed sequence tag (EST). Sequences from 898 independent clones were assembled in 555 clusters, representing the transcript profile of this tissue. The repertoire of possible proteins involved in feeding and host interaction processes of the leech corresponded to 10.6% of all identified transcripts (67 clusters), being the carbonic anhydrases (30%), several coagulation inhibitors (25%) and hemerythrin-like molecules (19%), the major components. Among the 387 clusters matching cellular proteins, the majority represents molecules involved in gene and protein expression, reflecting a high specialization of this tissue for protein synthesis. Our H. depressa dbEST was also compared to those from other blood-feeding organisms, providing evidences that among the secreted proteins, the coagulation inhibitors present a profile very characteristic of this animal class.
Faria
Fernanda
F
Laboratório de Bioquímica e Biofísica, Instituto Butantan, Av. Vital Brazil, 1500, CEP: 05503-900, São Paulo, Brazil.
Junqueira-de-Azevedo
Inácio de L M
Ide L
Ho
Paulo L
PL
Sampaio
Misako U
MU
Chudzinski-Tavassi
Ana M
AM
D000595:Amino Acid Sequence; D000818:Animals; D016000:Cluster Analysis; D003029:Coagulants / Q000037:antagonists & inhibitors; D017124:Conserved Sequence; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D015723:Gene Library; D007865:Leeches / Q000235:genetics*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D003029:Coagulants; D018076:DNA, Complementary; D012471:Salivary Proteins and Peptides