title: Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil.
abstract: The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.
authors.affiliation: Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, ICC Sul, 70919-900 Brasília, DF, Brazil.
authors.forename: Regina M S
authors.initials: RM
authors.lastname: Amorim
authors.forename: Claudiner P
authors.initials: CP
authors.lastname: Oliveira
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Wyant
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Cerqueira
authors.forename: Geni N L
authors.initials: GN
authors.lastname: Câmara
authors.forename: Luciano S
authors.initials: LS
authors.lastname: Flores
authors.forename: Regina M B
authors.initials: RM
authors.lastname: Martins
authors.forename: Cláudia R F
authors.initials: CR
authors.lastname: Martins
mesh_terms: D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000276:immunology; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018937:Hepatitis C Antibodies