[Testing of some grapevine cultivars for the presence of crown gall disease agent and fanleaf and leafroll viruses].
Some rootstocks and scion cultivars grown in Ukraine have been tested for the presence of tumorigenic agrobacteria, grapevine fanleaf and grapevine leafroll viruses of the 1st and 3rd serotypes by polymerase chain reaction and enzyme-linked immunosorbent assay. The conditions of polymerase chain reaction were modified in the course of investigations. It was shown that the certified material as well as regular planting material may be infected by the agent of crown gall disease. Certified grapevine material was free from grapevine fanleaf and grapevine leafroll viruses; at the same time the regular planting material was infected by both viruses. Our data indicate the necessity of including Agrobacterium vitis in the list of pathogens that should be tested in the process of certified planting material production.
Milkus
B N
BN
Konup
L O
LO
Zhun'ko
I D
ID
Limans'ka
N V
NV
D010941:Plant Tumors / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification*; D027843:Vitis / Q000821:virology*