Observations on the microcirculation of the human burn wound using orthogonal polarization spectral imaging.
Orthogonal polarization spectral imaging (OPS) utilizes the illumination of the tissue with polarized light within the haemoglobin spectrum. We report here on OPS for the assessment of the skin microcirculation non-invasively through the surface of the human burn wound. This allows inspection of individual capillaries of the cutaneous microcirculation and flow through these vessels in real time. Two distinct microcirculatory patterns were seen. Superficial burns had small visible dermal capillaries studied throughout the field of view. The flow of individual erythrocytes through these capillaries was clearly visible in real-time. Conversely, deep burns showed large thrombosed vessel coursing in a criss-cross fashion. There was marked difference between the mean optical densities for normal skin and superficial burns (65.8+/-15.6 and 64+/-14.6, respectively) and deep burns (131.2+/-31.1). These findings indicate that OPS may have utility in the assessment of cutaneous microcirculation in burns.
Milner
Stephen M
SM
The Plastic Surgery Institute, Southern Illinois University School of Medicine, P.O. Box 19653, Springfield, IL 62794-9653, USA. smilner@siumed.edu
Bhat
Satyanarayan
S
Gulati
Salil
S
Gherardini
Giulio
G
Smith
Christopher E
CE
Bick
Roger J
RJ
D002056:Burns / Q000473:pathology / Q000503:physiopathology*; D002196:Capillaries / Q000473:pathology / Q000503:physiopathology; D004912:Erythrocytes / Q000502:physiology; D006801:Humans; D008833:Microcirculation; D008859:Microscopy, Polarization; D055095:Optics and Photonics; D012867:Skin / Q000098:blood supply*; D013927:Thrombosis / Q000209:etiology / Q000473:pathology