title: [A sort of meliorative method for testing DNA from sperm smear].
abstract: OBJECTIVE
  To establish an effective method for testing DNA from the sperm smear.
  
  
  METHODS
  We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA. The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit.
  
  
  RESULTS
  Although there were a few sperm cells on the slide, it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction.
  
  
  CONCLUSIONS
  The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case.
authors.affiliation: Institute of Criminal Science,Public Security Bureau of Yancheng City, Yancheng 224002, China.
authors.forename: Yao-dong
authors.initials: YD
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.forename: Yue-yong
authors.initials: YY
authors.lastname: Chen
mesh_terms: D000328:Adult; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011902:Rape; D013048:Specimen Handling; D013094:Spermatozoa / Q000737:chemistry*; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D004247:DNA