Genetic diversity of multidrug-resistant Mycobacterium tuberculosis isolates and identification of 11 novel rpoB alleles in Taiwan.
Of 162 multidrug-resistant Mycobacterium tuberculosis isolates from Taiwan, 60.5% were found to belong to the Beijing family on the basis of spoligotyping results. IS6110 restriction fragment length polymorphism fingerprinting showed genetic diversity among the multidrug-resistant isolates. Furthermore, 90.1% of the multidrug-resistant isolates had mutations in the rpoB gene, and 11 novel alleles were recognized.
Jou
Ruwen
R
Reference Laboratory of Mycobacteriology, Center for Disease Control, Department of Health, Taiwan, 161 Kun-Yang Street, Nan-Kang, Taipei 115, Taiwan. rwj@cdc.gov.tw
Chen
Huang-Yau
HY
Chiang
Chen-Yuan
CY
Yu
Ming-Chih
MC
Su
Ih-Jen
IJ
D000483:Alleles; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit