[In vivo ectopic osteogenesis of nacre/polylactic acid artificial bone combined with allogenic osteoblasts].
OBJECTIVE
To study the mechanism of ectopic osteogenesis of nacre/Polylactic acid (N/P) artificial bone combined with allogenic osteoblasts, and to explore the possibility as a scaffold material of bone tissue engineering.


METHODS
The allogenic osteoblasts seeded onto N/P artificial bone were co-cultured in vivo 1 week. The N/P artificial bone with allogenic osteoblasts were implanted subcutaneously into the left back sites of the New Zealand white rabbits in the experimental group and the simple N/P artificial bone into the right ones in the control group. The complexes were harvested and examined by gross observation, histologic analysis and immunohistochemical investigation 2, 4 and 8 weeks after implantation respectively.


RESULTS
In experimental group, the osteoid formed after 4 weeks, and the mature bone tissue with bone medullary cavities formed after 8 weeks; but in control group there was no new bone formation instead of abundant fibrous tissue after 4 weeks, and more fibrous tissue after 8 weeks.


CONCLUSIONS
N/P artificial bone can be used as an optical scaffold material of bone tissue engineering.
Tang
Huanzhang
H
Department of Orthopaedic Surgery, Nanfang Hospital, the First Military Medical University, Guangzhou Guangdong, 510515, PR China. tanghuanzhang@sina.com
Chen
Jianting
J
Jin
Dadi
D
Yang
Chunlu
C
Tan
Xiaoyun
X
D000818:Animals; D018786:Bone Substitutes; D016025:Bone Transplantation; D002119:Calcium Carbonate; D002478:Cells, Cultured; D005240:Feasibility Studies; D019344:Lactic Acid; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000637:transplantation*; D010012:Osteogenesis; D011091:Polyesters; D011108:Polymers; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous
D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D002119:Calcium Carbonate