[Apoptosis of laryngeal carcinoma cell line Hep2 induced by tumor-specific expression of TRAIL gene].
OBJECTIVE
To explore targeted gene therapy of tumor by using the combination of TRAIL gene with the telomerase promoter.


METHODS
TRAIL gene with an IL-2 signal peptide was constructed by PCR and cloned into vector pGL3-181hTERT downstream of hTERT promoter to form an eukaryotic expressing vector. Hep2 cells were transfected by the recombinant vector and apoptosis of the transfected cells was evaluated by trypan-blue exclusion and the agarosegel electrophoresis of DNA.


RESULTS
We successfully constructed a recombinant eukaryotic expression vector for TRAIL gene.The expressed product significantly induced the apoptosis of Hep2 cells.


CONCLUSIONS
The recombinant eukaryotic expression vector pGL3-181hTERT/TRAIL was successfully constructed, which provides the possibility for gene therapy of tumor.
Chen
Jian-qiu
JQ
Department of Otorhinolaryngology,Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Cheng
Shi-yin
SY
Zhang
Hui-zhong
HZ
Han
Ming-kun
MK
Zhang
Li
L
D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics*; D019098:Telomerase / Q000235:genetics; D014162:Transfection
D018076:DNA, Complementary; D053221:TNF-Related Apoptosis-Inducing Ligand; D019098:Telomerase