A synthetic divalent cholera toxin glycocalix[4]arene ligand having higher affinity than natural GM1 oligosaccharide.
A high-affinity ligand of cholera toxin, the divalent glycocalix[4]arene 1, was obtained by exploiting a combination of structure-based design of glycomimetic monovalent ligands and affinity enhancements by multivalent presentation through a calix[4]arene scaffold. It exhibits a slightly higher affinity for the toxin than its natural ligand, the GM1 oligosaccharide.
Arosio
Daniela
D
Università di Milano, Dipartimento di Chimica Organica e Industriale and Centro di Eccellenza CISI, via Venezian 21, 20133 Milano, Italy.
Fontanella
Marco
M
Baldini
Laura
L
Mauri
Laura
L
Bernardi
Anna
A
Casnati
Alessandro
A
Sansone
Francesco
F
Ungaro
Rocco
R
D047250:Calixarenes / Q000737:chemistry* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002772:Cholera Toxin / Q000737:chemistry* / Q000378:metabolism; D019276:Glycocalyx / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism
D008024:Ligands; D009844:Oligosaccharides; D010636:Phenols; C121325:calix(4)arene; D047250:Calixarenes; C037563:G(M1)-oligosaccharide; D002772:Cholera Toxin