title: Differential protein expression in human gliomas and molecular insights.
abstract: Gliomas are the most common of the primary intracranial tumors with astrocytomas constituting about 40%. Using clinically and histologically assessed astrocytomas, we have studied their protein profiles using a two-dimensional gel electrophoresis-mass spectrometry approach and identified differentially expressed proteins which may be useful molecular indicators to understand these tumors. Examination of the protein profiles of 27 astrocytoma samples of different grades revealed 72 distinct, differentially expressed proteins belonging to various functional groups such as cytoskeleton and intermediate filament proteins, heat shock proteins (HSPs), enzymes and regulatory proteins. Based on the consistency of their differential expression, 29 distinct proteins could be short-listed and may have a role in the pathology of astrocytomas. Some were found to be differentially expressed in both Grade III and IV astrocytomas while others were associated with a particular grade. A notable observation was underexpression of Prohibitin, a potential tumor suppressor protein, Rho-GDP dissociation inhibitor, Rho-GDI, a regulator of Rho GTPases and HSPs as well as destabilization of glial fibrillary acidic protein, GFAP, major protein of the glial filaments, in Grade III malignant tumors. We attempt to explain glioma malignancy and progression in terms of their combined role.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: Vaibhav C
authors.initials: VC
authors.lastname: Chumbalkar
authors.forename: Chanumolu
authors.initials: C
authors.lastname: Subhashini
authors.forename: Vishnu M
authors.initials: VM
authors.lastname: Dhople
authors.forename: Curam S
authors.initials: CS
authors.lastname: Sundaram
authors.forename: Medicharla V
authors.initials: MV
authors.lastname: Jagannadham
authors.forename: Kafnam N
authors.initials: KN
authors.lastname: Kumar
authors.forename: Pasumarti N B S
authors.initials: PN
authors.lastname: Srinivas
authors.forename: Ravinutala
authors.initials: R
authors.lastname: Mythili
authors.forename: Mulla K
authors.initials: MK
authors.lastname: Rao
authors.forename: Mahesh J
authors.initials: MJ
authors.lastname: Kulkarni
authors.forename: Sridevi
authors.initials: S
authors.lastname: Hegde
authors.forename: Alangar S
authors.initials: AS
authors.lastname: Hegde
authors.forename: Cini
authors.initials: C
authors.lastname: Samual
authors.forename: Vani
authors.initials: V
authors.lastname: Santosh
authors.forename: Lalji
authors.initials: L
authors.lastname: Singh
authors.forename: Ravi
authors.initials: R
authors.lastname: Sirdeshmukh
mesh_terms: D001254:Astrocytoma / Q000378:metabolism*; D015153:Blotting, Western; D049109:Cell Proliferation; D018450:Disease Progression; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D013058:Mass Spectrometry; D018832:Molecular Chaperones; D009369:Neoplasms / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D014357:Trypsin / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D018832:Molecular Chaperones; D014357:Trypsin