title: Prevalence of genosubtypes (PorA types) of serogroup B invasive meningococcus in the north of Spain from 2000 to 2003.
abstract: The composition of new vaccines against Neisseria meningitidis serogroup B is based on differences in the variable regions VR1 and VR2 of the class 1 outer-membrane protein (PorA) of meningococci. Genosubtyping of 96 N. meningitidis B isolates from blood or cerebrospinal fluid from 2000 to 2003 in the north of Spain allowed characterization of all the strains. Twenty-six genosubtypes or distinct PorA types were obtained. The most prevalent were P1.5-1, 10-8 (20 strains), P1.19, 15 (14 strains), P1.22, 9 (11 strains) and P1.5, 2 (nine strains), while 17 genosubtypes were represented by only one or two strains. The wide diversity of genosubtypes observed and their differences compared with those found in other regions reveals the difficulty in designing a useful outer-membrane vesicle vaccine applicable to different regions of the world.
authors.affiliation: Microbiology Department, Hospital Donostia and Basque Country Meningococcal Reference Centre, Paseo Dr Beguiristain s/n, 20014 San Sebastián (Gipuzkoa), Spain 2Microbiology Laboratory, Hospital Txagorritxu, Vitoria, Spain 3Microbiology Laboratory, Hospital Galdácano, Galdácano, Spain 4Microbiology Department, Hospital Basurto, Bilbao, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Vicente
authors.affiliation: Microbiology Department, Hospital Donostia and Basque Country Meningococcal Reference Centre, Paseo Dr Beguiristain s/n, 20014 San Sebastián (Gipuzkoa), Spain 2Microbiology Laboratory, Hospital Txagorritxu, Vitoria, Spain 3Microbiology Laboratory, Hospital Galdácano, Galdácano, Spain 4Microbiology Department, Hospital Basurto, Bilbao, Spain.
authors.forename: Olatz
authors.initials: O
authors.lastname: Esnal
authors.affiliation: Microbiology Department, Hospital Donostia and Basque Country Meningococcal Reference Centre, Paseo Dr Beguiristain s/n, 20014 San Sebastián (Gipuzkoa), Spain 2Microbiology Laboratory, Hospital Txagorritxu, Vitoria, Spain 3Microbiology Laboratory, Hospital Galdácano, Galdácano, Spain 4Microbiology Department, Hospital Basurto, Bilbao, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Michaus
authors.affiliation: Microbiology Department, Hospital Donostia and Basque Country Meningococcal Reference Centre, Paseo Dr Beguiristain s/n, 20014 San Sebastián (Gipuzkoa), Spain 2Microbiology Laboratory, Hospital Txagorritxu, Vitoria, Spain 3Microbiology Laboratory, Hospital Galdácano, Galdácano, Spain 4Microbiology Department, Hospital Basurto, Bilbao, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: López de Goicoechea
authors.affiliation: Microbiology Department, Hospital Donostia and Basque Country Meningococcal Reference Centre, Paseo Dr Beguiristain s/n, 20014 San Sebastián (Gipuzkoa), Spain 2Microbiology Laboratory, Hospital Txagorritxu, Vitoria, Spain 3Microbiology Laboratory, Hospital Galdácano, Galdácano, Spain 4Microbiology Department, Hospital Basurto, Bilbao, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Cisterna
authors.affiliation: Microbiology Department, Hospital Donostia and Basque Country Meningococcal Reference Centre, Paseo Dr Beguiristain s/n, 20014 San Sebastián (Gipuzkoa), Spain 2Microbiology Laboratory, Hospital Txagorritxu, Vitoria, Spain 3Microbiology Laboratory, Hospital Galdácano, Galdácano, Spain 4Microbiology Department, Hospital Basurto, Bilbao, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Pérez-Trallero
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006801:Humans; D015994:Incidence; D008585:Meningitis, Meningococcal / Q000175:diagnosis* / Q000453:epidemiology; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D018272:Porins / Q000235:genetics*; D013030:Spain / Q000453:epidemiology
chemical_list: D018272:Porins; C057699:porin protein, Neisseria