Suppression of anti-TROSY lines in a sensitivity enhanced gradient selection TROSY scheme.
A simple modification of the TROSY pulse transfer scheme, suggested by Yang and Kay [J. Biomol. NMR 13 (1999) 3-10], is proposed which results in the suppression of unwanted anti-TROSY lines without any extra loss in sensitivity. The higher sensitivity of this TROSY transfer scheme therefore becomes available for 2D [15N, 1H] TROSY correlation and 3D/4D 15N separated NOESY type experiments where complete suppression of the broad anti-TROSY lines is essential.
Nietlispach
Daniel
D
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1 GA, UK. dn0@bio.cam.ac.uk
D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
D011506:Proteins