[In vitro study of the effect of 11 kinds of natural drugs on the growth and acid production of Lactobacillus].
OBJECTIVE
To study the effect of 11 different natural drugs on the growth and acid production of Lactobacillus, as a preparation for screening an effective agent to mediate the balance of oral microflora.


METHODS
Lactobacillus AC413 was chosen as the experimental bacterium. Eleven kinds of drugs, such as Rhizoma Chuanxiong, Surgentodoxa cuneata and Galla Chinensis were extracted by means of maceration, percolation and reflux extraction. The values of MIC of various extracts were measured. Then, different experimental media containing various extracts were prepared. The concentration of the extracts was lower than the MIC of the drug and the initial pH of the medium was 7.4. Lactobacillus was cultured in the medium for 48 hours, and finally the rest pH was measured. One-way ANOVA was used for statistical analysis.


RESULTS
When the concentration of the drugs was lower than 8.000mg/ml, Tea polyphenols, Catechu, Galla Chinensis, Radix et Rhizoma Rhei and Nidus Vespae can inhibit the growth of Lactobacillus effectively. Tea polyphenols, Nidus Vespae, Radix Scuteilariae, Galla Chinensis and Surgentodoxa cuneata can inhibit the acid production of Lactobacillus effectively, Rhizoma Chuanxiong, Radix et Rhizoma Rhei, Semen Arecae and Catechu have no preliminary effect on it, but Surgentodoxa Cuneata and Radix Angelicae Pubescentis can increase it.


CONCLUSIONS
Tea polyphenols, Catechu, Galla Chinensis, Radix et Rhizoma Rhei and Nidus Vespae can inhibit the growth of Lactobacillus effectively, and Tea polyphenols, Radix et Rhizoma Rhei, Nidus Vespae, Radix Scuteilariae, Galla Chinensis and Surgentodoxa Cuneata can inhibit the acid production of Lactobacillus effectively.
Huang
Zheng-Wei
ZW
Department of Endodontics, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University. Shanghai 200011, China. zhoumxd@yahoo.com
Zhou
Xue-Dong
XD
Xiao
Yue
Y
Liu
Tian-Jia
TJ
Li
Ji-Yao
JY
D000143:Acids / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008826:Microbial Sensitivity Tests
D000143:Acids; D004365:Drugs, Chinese Herbal