title: The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice.
abstract: We have investigated to develop novel vaccines against SARS CoV using cDNA constructs encoding the structural antigen; spike protein (S), membrane protein (M), envelope protein (E), or nucleocapsid (N) protein, derived from SARS CoV. Mice vaccinated with SARS-N or -M DNA using pcDNA 3.1(+) plasmid vector showed T cell immune responses (CTL induction and proliferation) against N or M protein, respectively. CTL responses were also detected to SARS DNA-transfected type II alveolar epithelial cells (T7 cell clone), which are thought to be initial target cells for SARS virus infection in human. To determine whether these DNA vaccines could induce T cell immune responses in humans as well as in mice, SCID-PBL/hu mice was immunized with these DNA vaccines. As expected, virus-specific CTL responses and T cell proliferation were induced from human T cells. SARS-N and SARS-M DNA vaccines and SCID-PBL/hu mouse model will be important in the development of protective vaccines.
authors.affiliation: Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone, Sakai, Osaka 591-8555, Japan. okm@kch.hosp.go.jp
authors.forename: Masaji
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authors.lastname: Okada
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authors.lastname: Takemoto
authors.forename: Yoshinobu
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authors.forename: Satomi
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authors.lastname: Hashimoto
authors.forename: Shigeto
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authors.lastname: Yoshida
authors.forename: Yukari
authors.initials: Y
authors.lastname: Fukunaga
authors.forename: Takao
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authors.forename: Yoko
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authors.lastname: Kita
authors.forename: Sachiko
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authors.lastname: Kuwayama
authors.forename: Yumiko
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authors.lastname: Muraki
authors.forename: Noriko
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authors.lastname: Takai
authors.forename: Chika
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authors.lastname: Okada
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authors.lastname: Sakaguchi
authors.forename: Izumi
authors.initials: I
authors.lastname: Furukawa
authors.forename: Kyoko
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authors.lastname: Yamada
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Tetsuo
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authors.lastname: Kase
authors.forename: Daphne E
authors.initials: DE
authors.lastname: Demello
authors.forename: J S M
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authors.lastname: Peiris
authors.forename: Pei-Jer
authors.initials: PJ
authors.lastname: Chen
authors.forename: Naoki
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authors.lastname: Yamamoto
authors.forename: Yoshiyuki
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authors.lastname: Yoshinaka
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nomura
authors.forename: Isao
authors.initials: I
authors.lastname: Ishida
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Sakatani
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014183:Transplantation, Heterologous; D019444:Vaccines, DNA / Q000235:genetics / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines