Effects of dioxin on gene expression in female reproductive system in the rat.
We analyzed the effects of a low dose of dioxin on gene expression in reproductive tissues or cells by microrepresentation differential analysis (micro-RDA) and DNA microarray analysis, and identified more than one hundred dioxin-sensitive genes in cultured rat granulosa cells, rat placentas and rat ovaries. It is well known that dioxin exerts effects mainly through Ah receptors which recognize the xenobiotic responsive element (XRE) in the promoter region of target genes. The present data, however, indicate that a low dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces or suppresses the expression of various genes, which have not been known as dioxin-sensitive. These data suggest that a low dose of dioxin has profound effects on the reproductive system.
Miyamoto
Kaoru
K
Department of Biochemistry, Fukui Medical University, Shimoaizuki 23, Matsuoka, Fukui 910-1193, Japan. kmiyamoto@fmsrsa.fukui-med.ac.jp
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D010920:Placenta / Q000187:drug effects*; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats
D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger