title: Human erythrocytes are affected by the organochloride insecticide chlordane.
abstract: Chlordane is a widely used organochlorine insecticide. In order to evaluate its perturbing effect upon the morphology of human erythrocytes it was caused to interact with human red cells and molecular models of cell membranes. These consisted in bilayers of dimyristoylphosphatidylethanolamine (DMPE) and of dimyristoylphosphatidylcholine (DMPC), representative of phospholipid classes located in the inner and outer monolayers of the erythrocyte membrane, respectively. Scanning electron microscopy (SEM) observations indicated that this pesticide induced a significant alteration in the shape of the erythrocytes as they changed their discoid shape to spherocytes. According to the bilayer couple hypothesis, the shape changes induced in erythrocytes by foreign molecules are due to differential expansion of their two monolayers. The fact that chlordane produced spherocytes would indicate that the pesticide was equally located in the outer and the inner moieties of the red cell membrane. This conclusion was supported by the results obtained from X-ray diffraction studies. These showed that the hydrophobic and polar head regions of DMPC bilayers were perturbed when the insecticide was in a 1:10 molar ratio with respect to the lipid. These results were confirmed by the fluorescence experiments performed in DMPC large unilamellar vesicles (LUV). Chlordane produced a sharp decrease in the anisotropy and general polarization parameters in the 0-0.1 mM range, implying an increase in the fluidity at the acyl chain and polar region of DMPC. On the other hand, the bilayer structure of DMPE was perturbed in a fashion similar to that observed by X-ray diffraction in DMPC, a fact that explains the morphological change induced by chlordane to the human erythrocytes.
authors.affiliation: Faculty of Chemical Sciences, Department of Polymers, University of Concepcion, Casilla 160-C, Concepcion, Chile. msuwalsk@udec.cl
authors.forename: M
authors.initials: M
authors.lastname: Suwalsky
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez
authors.forename: F
authors.initials: F
authors.lastname: Villena
authors.forename: C P
authors.initials: CP
authors.lastname: Sotomayor
mesh_terms: D000328:Adult; D002706:Chlordan / Q000009:adverse effects / Q000633:toxicity*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D007306:Insecticides / Q000009:adverse effects / Q000633:toxicity*; D008051:Lipid Bilayers / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010714:Phosphatidylethanolamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014961:X-Ray Diffraction
chemical_list: D007306:Insecticides; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D002706:Chlordan; D004134:Dimyristoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine