Influence of Sambucus nigra bark lectin on cell DNA under different in vitro conditions.
The biological activity of Sambucus nigra bark lectin on Chinese hamster cells in vitro was investigated by comet-assay and cytotoxicity testing. Mitogenic properties at the concentrations 0.063-0.25 microg/ml (but not higher) were found, and the induction of DNA breaks at concentrations 0.5 microg/ml and higher is demonstrated. S. nigra bark lectin at mitogenic concentrations decreased the level of nickel-induced DNA damage. The character and mechanism of this lectin protective activity was probably related to the induction of DNA reparation in the cells, decreasing nickel uptake in cells, and non-specific binding of nickel ions by protein molecules.
Macewicz
Larysa L
LL
Department of Human Genetics, Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnoho street, 150, Kyiv 03143, Ukraine.
Suchorada
Olena M
OM
Lukash
Lyubov L
LL
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D016466:CHO Cells; D020552:Comet Assay; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair; D008934:Mitogens / Q000494:pharmacology; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology; D037121:Plant Lectins / Q000494:pharmacology*; D054788:Ribosome Inactivating Proteins
D016587:Antimutagenic Agents; D008934:Mitogens; D037121:Plant Lectins; C050600:Sambucus nigra lectins; C022838:nickel chloride; D009532:Nickel; D054788:Ribosome Inactivating Proteins