The development of an algorithm for the mass spectral interpretation of phosphoproteins.
Extended from the peptide mapping method (Fenyo, D., Curr. Opin. Biotechnol. 2000, 11, 391-395) the proposed algorithm takes the mass information of a precursor ion to reconstruct all possible phosphorylated peptide sequences. The mass spectra of product ions from the corresponding precursor ion is then used and compared to the reconstructed sequences to deduce the most probable phosphoprotein. The proposed algorithm also predicts all possible combinations of phosphopeptides, which may serve as a clue for designing proper phosphorylation experiments to validate the existence of these peptides and the corresponding proteins.
Zhao
Yupeng
Y
Department of Chemical Engineering, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Lin
Yen-Han
YH
D000465:Algorithms; D030562:Databases, Protein; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D011499:Protein Processing, Post-Translational
D007477:Ions; D010455:Peptides; D010748:Phosphopeptides; D010750:Phosphoproteins