Ornithine decarboxylase and arginine decarboxylase gene transcripts are co-localized in developing tissues of Glycine max etiolated seedlings.
Unlike other eukaryotes, which synthesize polyamines (PA) only from ornithine, plants possess an additional pathway utilizing arginine as a precursor. In this study, we have identified cDNA clones coding for a Glycine max ornithine decarboxylase (ODC, EC 4.1.1.7) and an arginine decarboxylase (ADC, EC 4.1.1.19). Expression analysis using semi-quantitative RT-PCR approach revealed that both genes coding for enzymes involved in putrescine biosynthesis (ODC and ADC) were found in most plant organs examined. Significant expression levels of both genes were detected in root tips and hypocotyls. The spatial distribution of GmODC and GmADC transcripts in primary and lateral roots and hypocotyls revealed that these genes are co-expressed in expanding cells of cortex parenchyma, expanding cells of central cylinder in main roots and in developing tissues and expanding cells of soybean hypocotyls. The data point out a correlation of the expression patterns of GmODC and GmADC gene to certain physiological roles such as organ development and cell expansion.
Delis
Costas
C
Laboratory of Molecular Biology, Agricultural University of Athens, Georgios Aivalakis, Athens, Greece.
Dimou
Maria
M
Efrose
Rodica Catalina
RC
Flemetakis
Emmanouil
E
Aivalakis
Georgios
G
Katinakis
Panagiotis
P
D000595:Amino Acid Sequence; D002262:Carboxy-Lyases / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018546:Hypocotyl / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D011073:Polyamines / Q000378:metabolism; D011700:Putrescine; D036226:Seedlings / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D018076:DNA, Complementary; D018744:DNA, Plant; D011073:Polyamines; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; C019722:arginine decarboxylase; D011700:Putrescine