title: Use of microsatellite markers and gene dosage to quantify gene copy numbers in Candida albicans.
abstract: With microsatellite marker typing, the number of alleles must be known for calculation of allelic frequencies in the diploid Candida albicans for a given locus. We describe a gene dosage with a double real-time PCR. Such a dosage should also be useful in exploring the loss of heterozygosity in C. albicans.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Hôpital H. Mondor, 51 avenue du Maréchal DeLattre de Tassigny, 94010 Créteil, France.
authors.forename: J-M
authors.initials: JM
authors.lastname: Costa
authors.forename: O
authors.initials: O
authors.lastname: Eloy
authors.forename: F
authors.initials: F
authors.lastname: Botterel
authors.forename: G
authors.initials: G
authors.lastname: Janbon
authors.forename: S
authors.initials: S
authors.lastname: Bretagne
mesh_terms: D002176:Candida albicans / Q000235:genetics*; D018628:Gene Dosage; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction