Electroacupuncture stimulates hypothalamic aromatization.
We have previously reported that the repeated electroacupuncture (EA) stimulation significantly increased the concentrations of circulating estradiol and restored the depressed function of the hypothalamus-pituitary-ovary axis (HPOA) in ovariectomized (OVX) rats. We hypothesize that extragonadal aromatization in specific brain areas might be responsible for these changes. Thus, various assays, including radiometric assay, Western blot, and reverse transcriptase polymerase chain reaction (RT-PCR), were employed to determine the aromatization in the hypothalamus of rats that received both OVX and electroacupuncture (OVX + EA). The results showed that EA significantly increased the aromatase activity as well as the expressions of its mRNA and protein (P < 0.05) in the OVX rats. These results suggest that EA enhances brain aromatization, which might contribute to influence the function of gonadotropin-releasing hormone (GnRH) neurons and promote the hypofunction of the HPOA in the ovariectomized rats.
Zhao
Hong
H
Department of Neurobiology and Integrative Medicine, Institute of Acupuncture Research, Shanghai Medical Center of Fudan University, PO Box 291, 138 Yi-Xue-Yuan Road, 200032 Shanghai, P.R. China.
Tian
Zhan-Zhuang
ZZ
Chen
Bo-Ying
BY
D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D015153:Blotting, Western; D015671:Electroacupuncture; D004958:Estradiol / Q000378:metabolism; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007031:Hypothalamus / Q000201:enzymology / Q000378:metabolism*; D010052:Ovariectomy; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014621:Vagina / Q000166:cytology
D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D012313:RNA; D001141:Aromatase