Transfer of transformed chloroplasts from Nicotiana tabacum to the Lycium barbarum plants.
Plastid transformation is an attractive technology for obtaining crop plants with new useful characteristics and for fundamental researches of plastid functioning and nuclear-plastid interaction. The aim of our experiments was to obtain plants with Lycium barbarum nucleus and transformed Nicotiana tabacum plastids. Plastome of previously engineered transplastomic tobacco plants contains reporter uidA gene and selective aadA gene that confers resistance to antibiotics spectinomycin and streptomycin. Asymmetric somatic hybridization was performed for transferring transformed tobacco plastids from transplastomic tobacco plants into recipient L. barbarum wild type plants. Hybrid L. barbarum plants containing transformed tobacco plastome with active aadA and uidA genes were obtained as a result of the experiments. The work shows the possibility of obtaining transplastomic plants by transferring the transformed plastids to remote species by using somatic hybridization technology. The developed technique is especially effective for obtaining transplastomic plants that have low regeneration and transformation ability.
Sytnik
Ekaterina
E
Institute of Cell Biology & Genetic Engineering, Zabolotnoho street 148, Kyiv 03143, Ukraine. iicb@iicb.kiev.ua
Komarnytsky
Igor
I
Gleba
Yuri
Y
Kuchuk
Nikolay
N
D002736:Chloroplasts / Q000235:genetics* / Q000648:ultrastructure*; D018744:DNA, Plant / Q000737:chemistry; D006824:Hybridization, Genetic; D032305:Lycium / Q000235:genetics* / Q000648:ultrastructure*; D030821:Plants, Genetically Modified / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D014026:Nicotiana / Q000235:genetics* / Q000648:ultrastructure*; D014170:Transformation, Genetic
D018744:DNA, Plant