Environmental DNA fragment conferring early and increased sporulation and antibiotic production in Streptomyces species.
Here we describe the rep gene, isolated from an environmental DNA library, which when transformed into Streptomyces species resulted in increased production of secondary metabolites and accelerated sporulation. We show that Streptomyces lividans strains bearing rep are particularly useful as expression hosts for heterologous antibiotic production.
Martinez
Asuncion
A
Sanofi-Aventis Cambridge Genomics Center, Cambridge, Massachusetts, USA. chon@mit.edu
Kolvek
Steven J
SJ
Hopke
Joern
J
Yip
Choi Lai Tiong
CL
Osburne
Marcia S
MS
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004783:Environmental Microbiology; D015723:Gene Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology*; D048372:Streptomyces coelicolor / Q000235:genetics / Q000502:physiology; D048170:Streptomyces lividans / Q000235:genetics / Q000502:physiology; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins