Levels of L-selectin (CD62L) on human leukocytes in disseminated cryptococcosis with and without associated HIV-1 infection.
Patients with disseminated cryptococcosis typically have measurable levels of cryptococcal polysaccharide in serum samples but minimal leukocyte infiltration into infected tissues. In vitro data have shown that cryptococcal polysaccharide induces L-selectin (CD62L) shedding from leukocytes. To assess shedding in vivo, we compared leukocyte L-selectin levels in human immunodeficiency virus (HIV) type 1-negative and -positive subjects with and without circulating cryptococcal polysaccharide. Results showed that subjects with cryptococcal polysaccharide in serum samples have significantly lower percentages of neutrophils, monocytes, and CD3+ T cells with L-selectin on their surfaces than do healthy subjects, regardless of HIV status. There was significantly more soluble L-selectin in serum samples from subjects with cryptococcosis than in those from uninfected subjects. Reduced L-selectin levels on leukocytes in subjects with circulating cryptococcal polysaccharide and increased serum levels of soluble L-selectin indicates that surface L-selectin shedding is a mechanism that likely explains reduced leukocyte extravasation into infected tissues of patients with disseminated cryptococcosis.
Jackson
Lydgia A
LA
University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Drevets
Douglas A
DA
Dong
Zhao-Ming
ZM
Greenfield
Ronald A
RA
Murphy
Juneann W
JW
D000328:Adult; D015331:Cohort Studies; D003453:Cryptococcosis / Q000097:blood / Q000150:complications* / Q000378:metabolism*; D015658:HIV Infections / Q000097:blood / Q000150:complications* / Q000821:virology; D006801:Humans; D019041:L-Selectin / Q000032:analysis* / Q000097:blood; D007962:Leukocytes / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000378:metabolism
D019041:L-Selectin