title: Efficient derivation of new human embryonic stem cell lines.
abstract: Human embryonic stem (hES) cells, unlike most cells derived from adult or fetal human tissues, represent a potentially unlimited source of various cell types for basic clinical research. To meet the increased demand for characterized hES cell lines, we established and characterized nine new lines obtained from frozen-thawed pronucleus-stage embryos. In addition, we improved the derivation efficiency from inner cell masses (to 47.4%) and optimized culture conditions for undifferentiated hES cells. After these cell lines had been maintained for over a year in vitro, they were characterized comprehensively for expression of markers of undifferentiated hES cells, karyotype, and in vitro/in vivo differentiation capacity. All of the cell lines were pluripotent, and one cell line was trisomic for chromosome 3. Improved culture techniques for hES cells should make them a good source for diverse applications in regenerative medicine, but further investigation is needed of their basic biology.
authors.affiliation: Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Seoul 157-280, Korea.
authors.forename: Sun Jong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jeoung Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Jong Hyuk
authors.initials: JH
authors.lastname: Park
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Jin Mee
authors.initials: JM
authors.lastname: Kim
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Yoon
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Song
authors.forename: Sung Il
authors.initials: SI
authors.lastname: Roh
authors.forename: Chul Geun
authors.initials: CG
authors.lastname: Kim
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques / Q000379:methods; D015925:Cryopreservation; D016172:DNA Fingerprinting; D033041:Embryo Research; D005347:Fibroblasts; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D016513:Mice, SCID; D039904:Pluripotent Stem Cells / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D013724:Teratoma / Q000473:pathology; D013736:Testicular Neoplasms / Q000473:pathology