Hypoglycaemic and antidiabetic activity of acetonic extract of Vernonia colorata leaves in normoglycaemic and alloxan-induced diabetic rats.
The aqueous extract of Vernonia colorata (Willd.) Drake (Composeae) leaves is used by African traditional medicine practitioners as a remedy for the treatment of diabetes. Our previous studies have shown the hypoglycaemic activity of the aqueous extract of Vernonia colorata leaves (300 mg/kg, per os) in normoglycaemic rats. The aim of the present study was to investigate the hypoglycaemic and antidiabetic activity of acetonic and hexanic extracts of the leaves of Vernonia colorata in order to further discriminate the type of extract which provides a better antidiabetic activity. Experiments were performed in normoglycaemic and alloxan-induced diabetic rats. The acetonic extract of the leaves of Vernonia colorata (AELVC) (100 mg/kg, per os) induced a significant decrease of blood glucose in normoglycaemic rats. The glycaemia varied from 4.72+/-0.11 to 3.72+/-0.22 mmol/l (p<0.05, n=5) 3 h after AELVC administration per os. In contrast, the hexanic extract of the leaves of Vernonia colorata (HELVC) increased significantly the glycaemia in normoglycaemic rats. Like glibenclamide, AELVC has an antihyperglycaemic effect in oral glucose tolerance test (OGTT) and alloxan-induced diabetic rats. These results have shown that: (i) AELVC and HELVC have an opposite effect on basal blood glucose in normoglycaemic rats, suggesting that the mechanisms of action of both above-mentioned extracts are different; (ii) AELVC has also an antidiabetic activity in hyperglycaemic rat models.
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Laboratoire de Pharmacologie et de Physiologie, Faculté de Médecine et Pharmacie, Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal. guatayoro_sy@yahoo.fr
Cissé
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Nongonierma
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Sarr
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Mbodj
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Faye
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D000096:Acetone / Q000737:chemistry; D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D005951:Glucose Tolerance Test; D005905:Glyburide / Q000494:pharmacology / Q000627:therapeutic use; D006586:Hexanes / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification; D007003:Hypoglycemia / Q000139:chemically induced*; D016488:Medicine, African Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D035741:Vernonia / Q000737:chemistry*
D001786:Blood Glucose; D006586:Hexanes; D010936:Plant Extracts; D000096:Acetone; D005947:Glucose; D005905:Glyburide