title: Toxicological effects of acrylamide on rat testicular gene expression profile.
abstract: Toxicological effects of acrylamide on differential gene expression profile of rat testis were evaluated. Acrylamide induced morphological sperm defects, and decreased sperm concentration in cauda epididymis. Serum testosterone level and Leydig cell viability were also decreased dose-dependently, which resulted in decreased spermatogenesis. Acrylamide-induced histopathological lesions, such as formation of multinucleated giant cells and vacuolation, and numerous apoptotic cells were observed in seminiferous tubules. cDNA microarray analysis revealed that genes related to testicular-functions, apoptosis, cellular redox, cell growth, cell cycle, and nucleic acid-binding were up/down-regulated in testes isolated from acrylamide-treated group (60 mg/kg/day). Acrylamide toxicity appears to increase Leydig cell death and perturb gene expression levels, contributing to sperm defects and various abnormal histopathological lesions including apoptosis in rat testis.
authors.affiliation: Department of Biochemistry, College of Veterinary Medicine, Seoul National University, 151-742 Seoul, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Yang
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Yong
authors.initials: Y
authors.lastname: Jin
authors.forename: Jin-Hyang
authors.initials: JH
authors.lastname: Choi
authors.forename: Dong-Un
authors.initials: DU
authors.lastname: Han
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
authors.forename: Mun-Han
authors.initials: MH
authors.lastname: Lee
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Han
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004785:Environmental Pollutants / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: D004785:Environmental Pollutants; D020106:Acrylamide