title: Long-term bone marrow culture data are the most powerful predictor of peripheral blood progenitor cell mobilization in healthy donors.
abstract: OBJECTIVE
  There is wide interindividual variation in progenitor cell mobilization. The present study was aimed to analyze steady state hematopoiesis in healthy donors and its influence on hematopoietic progenitor cell (HPC) mobilization.
  
  
  METHODS
  Bone marrow (BM) was aspirated from 72 healthy donors prior to administration of recombinant human granulocyte colony-stimulating factor (G-CSF). Analyses of CD34+ cells and semisolid cultures as well as long-term cultures were performed from BM or leukapheresis products.
  
  
  RESULTS
  Male donors showed a higher number of BFU-E (p=0.007) and committed progenitors (p=0.05), a better stromal layer (p=0.02), and higher long-term bone marrow culture (LT-BMC) counts (p<0.05) when compared to those in female donors. When correlating the culture pattern of the BM with the data from the leukapheresis products, we observed that the number of the immature progenitors in BM correlated significantly with both the number of CD34 + cells and CFU-GM in the first leukapheresis. Univariate analysis revealed that the following variables had a beneficial impact on the number of CD34+ cells: male sex, body weight >73 Kg, G-CSF schedule and results of LT-BMC, although in the multivariate analysis only the number of CFU-GM obtained after LT-BMC showed a significant influence (p<0.001).
  
  
  CONCLUSIONS
  These results confirm the interindividual variation in HPC mobilization among healthy subjects, with LT-BMC counts being the most reliable predictor, expressing the behavior of the immature progenitors and their relationship with the microenvironment.
authors.affiliation: Department of Hematology, Hospital Clínico Universitario de Salamanca, Salamanca, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: López-Holgado
authors.forename: Carmen
authors.initials: C
authors.lastname: Pata
authors.forename: Eva
authors.initials: E
authors.lastname: Villarón
authors.forename: Fermín
authors.initials: F
authors.lastname: Sánchez-Guijo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Alberca
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martín
authors.forename: Mercedes
authors.initials: M
authors.lastname: Corral
authors.forename: Ignacio
authors.initials: I
authors.lastname: Sánchez-Abarca
authors.forename: Josè Antonio
authors.initials: JA
authors.lastname: Pérez-Simón
authors.forename: Jesús Fernando
authors.initials: JF
authors.lastname: San Miguel
authors.forename: Maria Consuelo
authors.initials: MC
authors.lastname: del Cañizo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002648:Child; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006410:Hematopoiesis; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods / Q000592:standards*; D006801:Humans; D007937:Leukapheresis / Q000592:standards; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D011446:Prospective Studies; D014019:Tissue Donors
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor