[Resonance Raman spectra of single red-cell from human blood].
Resonance Raman spectra of single red-cell from human blood are presented by different laser sources. It is reported that there is 1002 cm(-1) line of insensitive conformation of phenylalanine aromatic ring stretching and 1620 cm(-1) line of C-N breathing stretching band of pyrrole ring, which cause strong and sharp resonance lines when excited by 782 nm laser source. They are weaker and the intensity of other lines of high wave number is large and clear when excited by 514 nm laser source. But the intensity of lines of low wave number is strong and clear when excited by 782 nm laser source. At the same time, the authors got Raman spectra lines at different times after taking blood under the same laser source. When using 782 nm laser source, there is no difference except for 1601 cm(-1). There are a lot of weakened lines and lines shifting about 4-10 cm(-1) toward low wave number. The results indicate that Raman spectra may offer the experimental basis for studying structure, function and variability of single red-cell.
Yan
Xun-ling
XL
Physics Department of Liaocheng University, Liaocheng 252059, China.
Dong
Rui-xin
RX
Wang
Qiu-guo
QG
D002478:Cells, Cultured; D004867:Equipment Design; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007834:Lasers; D052898:Optical Tweezers / Q000706:statistics & numerical data; D013055:Spectrophotometry, Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014732:Vibration