title: High-rate acidophilic ferrous iron oxidation in a biofilm airlift reactor and the role of the carrier material.
abstract: In this study, the feasibility and engineering aspects of acidophilic ferrous iron oxidation in a continuous biofilm airlift reactor inoculated with a mixed culture of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans bacteria were investigated. Specific attention was paid to biofilm formation, competition between both types of bacteria, ferrous iron oxidation rate, and gas liquid mass transfer limitations. The reactor was operated at a constant temperature of 30 degrees C and at pH values of 0-1.8. Startup of the reactor was performed with basalt carrier material. During the experiments the basalt was slowly removed and the ferric iron precipitates formed served as a biofilm carrier. These precipitates have highly suitable characteristics as a carrier material for the immobilization of ferrous iron-oxidizing bacteria and dense conglomerates were observed. Lowering the pH (0.6-1) resulted in dissolution of the ferric precipitates and induced granular sludge formation. The maximum ferrous iron oxidation rate achieved in this study was about 145 molFe(2+)/m(3).h at a hydraulic residence time of 0.25 h. Optimal treatment performance was obtained at a loading rate of 100 mol/m(3).h at a conversion efficiency as high as 98%. Fluorescent in situ hybridization (FISH) studies showed that when the reactor was operated at high ferrous iron conversion (>85%) for 1 month, the desirable L. ferrooxidans species could out-compete A. ferrooxidans due to the low Fe(2+) and high Fe(3+) concentrations.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands. S.Ebrahim@TNW.TUDelft.nl
authors.forename: S
authors.initials: S
authors.lastname: Ebrahimi
authors.forename: F J
authors.initials: FJ
authors.lastname: Fernández Morales
authors.forename: R
authors.initials: R
authors.lastname: Kleerebezem
authors.forename: J J
authors.initials: JJ
authors.lastname: Heijnen
authors.forename: M C M
authors.initials: MC
authors.lastname: van Loosdrecht
mesh_terms: D042763:Acidithiobacillus / Q000378:metabolism*; D018441:Biofilms; D019149:Bioreactors; D005296:Ferrous Compounds / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D010084:Oxidation-Reduction
chemical_list: D005296:Ferrous Compounds