Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay.
Exposure to indoor air pollution (IAP) from the combustion of biomass fuels is an important cause of morbidity and mortality in developing countries. In the work discussed in this paper we evaluated the endocrine activity of soot particles from biomass fuels by using yeast bioassay. These pollutants could have beta-galactosidase activity with a relative potency (RP) about 10(-7)-10(-9) that of estradiol. Soot particles from wood and straw combustion only partially induced beta-galactosidase activity whereas others produced fully inductive activity in the yeast assay system. These pollutants did not have estrogen antagonist and progesterone agonist activity within the defined concentration range. However, these pollutants require 2-4 orders of magnitude higher IC50 to inhibit the activity of progesterone in a similar dose-response manner to mifepristone. We therefore propose that the endocrine activity of some environmental pollutants may be because of inhibition of the progesterone receptor (hPR). GC-MS results showed that substituted polycyclic aromatic hydrocarbon (PAH) compounds, substituted phenolic compounds and derivatives, aromatic carbonyl compounds, and phytosteroids in these soot particles may be mimicking endogenous hormones.
Wang
Jingxian
J
Donghu Experimental Station of the Lake Ecosystems, The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, PR China.
Xie
Ping
P
Kettrup
Antonius
A
Schramm
Karl-Werner
KW
D000393:Air Pollutants / Q000737:chemistry / Q000494:pharmacology*; D001681:Biological Assay; D002244:Carbon / Q000737:chemistry / Q000494:pharmacology*; D002681:China; D004736:Energy-Generating Resources; D004965:Estrogen Antagonists / Q000032:analysis / Q000494:pharmacology*; D004967:Estrogens / Q000819:agonists* / Q000032:analysis; D018547:Plant Stems; D006109:Poaceae; D011374:Progesterone / Q000819:agonists* / Q000032:analysis / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae; D014934:Wood
D000393:Air Pollutants; D004965:Estrogen Antagonists; D004967:Estrogens; D011374:Progesterone; D002244:Carbon