[Histological location of DMP1, OPN and BSP in cementum formation of mouse].
OBJECTIVE
To study the distribution of dentin matrix protein 1, osteopontin and bone sialoprotein during cementum formation and cementoblasts differentiation in mouse.


METHODS
Thirty six BALB/c postnatal mice were divided into four groups according to the developing stages, nine in each was subdivided into three parts randomly to examine the location of DMP1, OPN and BSP, a tooth developing study model was built and examined using histological methods correspondingly. PV two steps immunohistochemical assay was used to localize the distribution of the three regulatory factors timely and spatially. Repeated measures ANOVA and statistical analysis software of CMIAS were used to analyze the intensity of the dyed images.


RESULTS
There was a series expression of the three regulatory proteins. DMP1 was expressed in the dental follicle cells in day 5 of postnatal stages and negatively expressed when the cell began to differentiate; meanwhile, since day 10 and on OPN was positively located in cementoblasts until the root developed completely; but BSP was expressed in the same cell in day 15 to 20, and then decreased to be negative. There were significant differences statistically among the three factors in each stage observed in the study except OPN between day 15 and 25.


CONCLUSIONS
There are different locations timely and spatially among the three proteins, it hints that they play important different roles during the differentiation of cementoblasts and formation of cementum.
Li
Shu
S
Department of Periodontology, School of Stomatology, Shandong University. Jinan 250012, Shandong Province, China. yangps@sdu.edu.cn
Pan
Ke-Qing
KQ
Ge
Shao-Hua
SH
Xie
Rui-Yue
RY
Duan
Xue-Jing
XJ
Yang
Pi-Shan
PS
D000818:Animals; D002454:Cell Differentiation; D003739:Dental Cementum / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D053495:Osteopontin / Q000378:metabolism*
C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; C506896:Spp1 protein, mouse; D053495:Osteopontin