title: Expression of Ndrg2 in the rat frontal cortex after antidepressant and electroconvulsive treatment.
abstract: Although the therapeutic action of antidepressants most likely involves the regulation of serotonergic and noradrenergic signal transduction, no consensus has been reached concerning their precise molecular or cellular mechanisms of action. In the present study, we demonstrated that chronic treatment with a tricyclic antidepressant (imipramine) and a selective serotonin reuptake inhibitor (sertraline) reduced the expression of Ndrg2 mRNA and protein in the rat frontal cortex. Ndrg2 is a member of the N-Myc downstream-regulated genes. Interestingly, repeated ECT also significantly decreased Ndrg2 expression in this region of the brain. These data suggest that Ndrg2 may be a common functional molecule that is decreased after antidepressant treatment and ECT. Although, the functional role of Ndrg2 in the central nervous system remains unclear, our findings suggest that Ndrg2 may be associated with treatment-induced adaptive neural plasticity in the brain, a chronic target of antidepressant action. In conclusion, we have identified Ndrg2 as a candidate target molecule of antidepressants and ECT.
authors.affiliation: Department of Pharmacology, Showa University School of Pharmaceutical Sciences, Tokyo, Japan.
authors.forename: Kou
authors.initials: K
authors.lastname: Takahashi
authors.forename: Misa
authors.initials: M
authors.lastname: Yamada
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Ohata
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Momose
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Higuchi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Honda
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage*; D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D004334:Drug Administration Schedule; D004597:Electroshock / Q000379:methods*; D005625:Frontal Lobe / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D015870:Gene Expression / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014162:Transfection / Q000379:methods
chemical_list: D000928:Antidepressive Agents; C507213:Ndrg2 protein, rat; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger