title: [Expression of interleukin 2 and IL-2 receptor after implanted tissue engineered bones constructed with allogeneic marrow stromal stem cells and bio-derived materials in rhesus monkeys].
abstract: OBJECTIVE
  To explore the feasibility of allogeneic marrow stromal stem cells (MSCs) as seed cells to construct tissue engineered bone by detecting the expressions of interleukin 2 (IL-2) and IL-2 receptor in rhesus monkeys after implanting these tissue engineered bones.
  
  
  METHODS
  Engineered bones were constructed with osteoblasts which derived from allogeneic MSCs and bio-derived materials in vitro, and then were implanted to bridge 2.5 cm segmental bone defects of left radius in 15 rhesus monkeys as experimental group, bio-derived materials only were implanted to bridge same size defects of right radius as control group. Every 3 monkeys were sacrificed in the 1st, the 2nd, the 3rd, the 6th and the 12th weeks postoperatively and the expressions of IL-2 and IL-2 receptor in blood and graft samples were detected quantitatively by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  There was no significant difference in the contents of IL-2 and its receptor between 2 groups (P>0.05). The contents of IL-2 and its receptor increased from the 2nd week and maintained high level from the 2nd to the 6th week, but decreased after 6 weeks.
  
  
  CONCLUSIONS
  Tissue engineered bones constructed with allogeneic MSCs and bio-derived materials show low immunogenicity. Allogeneic MSCs may be used as seed cells to construct tissue engineered bone.
authors.affiliation: Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Yang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yang
authors.forename: Xiuqun
authors.initials: X
authors.lastname: Li
authors.forename: Yueting
authors.initials: Y
authors.lastname: Zhou
authors.forename: Fan
authors.initials: F
authors.lastname: Lin
authors.forename: Tingwu
authors.initials: T
authors.lastname: Qin
authors.forename: Jingcong
authors.initials: J
authors.lastname: Luo
authors.forename: Huiqi
authors.initials: H
authors.lastname: Xie
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D016026:Bone Marrow Transplantation; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007376:Interleukin-2 / Q000097:blood / Q000378:metabolism*; D008253:Macaca mulatta; D011884:Radius / Q000293:injuries / Q000601:surgery; D015375:Receptors, Interleukin-2 / Q000097:blood / Q000378:metabolism*; D017154:Stromal Cells / Q000637:transplantation; D023822:Tissue Engineering / Q000379:methods; D014184:Transplantation, Homologous
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D007376:Interleukin-2; D015375:Receptors, Interleukin-2