Application of synthetic 8-kD and recombinant GP50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay.
The gold standard serodiagnostic assay for cysticercosis and neurocysticercosis, diseases caused by the metacestode of Taenia solium, uses lentil lectin-purified glycoprotein (LLGP) in a Western blot assay. We tested two antigens derived from LLGP, synthetic TS18var1 (sTS18var1) and recombinant GP50 antigen (rGP50), in an enzyme-linked immunosorbent assay (ELISA) using serum and cerebrospinal fluid (CSF) samples. The sensitivity for serum and CSF was 94.7% and 100% for rGP50 and 90.4% and 90.2% for sTS18var1, respectively. The specificity for serum and CSF samples was 93.8% and 100% for rGP50 and 90.3% and 98.0% for sTS18var1, respectively. The use of these antigens individually or combined as a diagnostic antigen cocktail eliminates the need for purification of antigens from parasite material and offers the advantage of using a simple and quantitative ELISA format.
Bueno
Edneia C
EC
Faculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil. ecbueno@ccs.univali.br
Scheel
Christina M
CM
Vaz
Adelaide J
AJ
Machado
Luis R
LR
Livramento
José A
JA
Takayanagui
Osvaldo M
OM
Tsang
Victor C W
VC
Hancock
Kathy
K
D000818:Animals; D000906:Antibodies / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009419:Nerve Tissue Proteins / Q000097:blood* / Q000235:genetics; D020019:Neurocysticercosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D011994:Recombinant Proteins / Q000097:blood; D012016:Reference Values; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000906:Antibodies; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C052914:brain synaptic membrane glycoprotein gp 50