Necrosis of infectious pancreatic necrosis virus (IPNV) infected cells rarely is preceded by apoptosis.
Infectious pancreatic necrosis virus (IPNV)-infected cells were labeled with Annexin V and propidium iodide in order to determine the proportion of cells, which developed necrosis and/or apoptosis during the time course of infection. Contrasting with earlier reports, we found that at any time during IPNV multiplication cycle, the percentage of apoptotic cells never exceeded the 12% of the whole population of the infected cells. In addition, the percentage of necrotic cells increased continuously until reaching the 75% of the infected cells at 15 h post infection. Apoptotic cells were also identified by in situ terminal deoxynucleotidyl transferase-mediated BrdUTP nick end labeling (TUNEL). Our results are in accordance with the idea that apoptosis rarely precedes necrosis.
Espinoza
Juan Carlos
JC
Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Casilla 50 30, Universidad de Valparaíso, Valparaíso, Chile.
Cortés-Gutierrez
Marcia
M
Kuznar
Juan
J
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D020287:In Situ Nick-End Labeling; D018061:Infectious pancreatic necrosis virus / Q000502:physiology*; D009336:Necrosis; D011419:Propidium / Q000378:metabolism; D012474:Salmon; D013194:Staining and Labeling; D019065:Virus Assembly
D017304:Annexin A5; D011419:Propidium