title: Effects of mGlu1 and mGlu5 receptor antagonists on negatively reinforced learning.
abstract: Effects on aversive learning of the novel highly selective mGlu5 receptor antagonist [(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine (MTEP) and mGlu1 receptor antagonist (3-ethyl-2-methyl-quinolin-6-yl)-(4-methoxy-cyclohexyl)-methanone methanesulfonate (EMQMCM) were tested, after systemic administration, in the passive avoidance (PA) and fear potentiated startle (FPS) paradigms. Both MTEP at 10 mg/kg and EMQMCM at 5 and 10 mg/kg, given 30 min before training, impaired acquisition of the passive avoidance response (PAR). Co-administration of MTEP and EMQMCM at doses ineffective when administered alone, produced anterograde amnesia when given 30 min before the acquisition phase. Neither EMQMCM (5 mg/kg) nor MTEP (10 mg/kg) impaired retention of the PAR after direct post-training injections. EMQMCM (5 mg/kg), but not MTEP (10 mg/kg) blocked the PAR when given 30 min before testing. Pre-training administration of MTEP at doses of 2.5 and 5 mg/kg inhibited fear conditioning in the FPS when tested 24 h later. In contrast, EMQMCM was ineffective. Our findings suggest diverse involvement of mGlu1 and mGlu5 receptors in negatively reinforced learning.
authors.affiliation: Preclinical R & D, Merz Pharmaceuticals, Frankfurt am Main, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Gravius
authors.forename: M
authors.initials: M
authors.lastname: Pietraszek
authors.forename: D
authors.initials: D
authors.lastname: Schäfer
authors.forename: W J
authors.initials: WJ
authors.lastname: Schmidt
authors.forename: W
authors.initials: W
authors.lastname: Danysz
mesh_terms: D020324:Amnesia, Anterograde / Q000139:chemically induced; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D005239:Fear; D008297:Male; D008568:Memory / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology*; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000037:antagonists & inhibitors* / Q000502:physiology*; D013216:Reflex, Startle; D012054:Reinforcement, Psychology; D013844:Thiazoles / Q000494:pharmacology*
chemical_list: C502015:(3-ethyl-2-methyl-quinolin-6-yl)-(4-methoxycyclohexyl)methanone methanesulfonate; C472365:3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine; D011725:Pyridines; D011804:Quinolines; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; D013844:Thiazoles; C104077:metabotropic glutamate receptor type 1