title: Micromonospora mirobrigensis sp. nov.
abstract: An actinomycete strain was recovered from a pond where radon is known to be dissolved. A polyphasic study was undertaken to identify the new isolate. The 16S rRNA gene sequence of strain WA201(T) showed closest similarity to the type strains of Micromonospora carbonacea (98.5 %) and Micromonospora matsumotoense (98.1 %). The chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Micromonospora. DNA-DNA relatedness values supported the classification of this isolate as a novel species. A number of physiological and biochemical tests were able to distinguish strain WA201(T) from its closest phylogenetic neighbours. Therefore, it is proposed that isolate WA201(T) (=DSM 44830(T)=LMG 22229(T)) be considered the type strain representing a novel species, Micromonospora mirobrigensis sp. nov.
authors.affiliation: Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
authors.forename: Martha E
authors.initials: ME
authors.lastname: Trujillo
authors.affiliation: Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernández-Molinero
authors.affiliation: Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Mateos
authors.affiliation: Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
authors.forename: Eustoquio
authors.initials: E
authors.lastname: Martínez-Molina
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D008847:Micromonospora / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011886:Radon; D017422:Sequence Analysis, DNA; D014875:Water Pollutants, Radioactive
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014875:Water Pollutants, Radioactive; D011886:Radon