Macrophage colony-stimulating factor (M-CSF), as well as granulocyte colony-stimulating factor (G-CSF), accelerates neovascularization.
It has been reported that bone marrow cells (BMCs) differentiate into endothelial cells of blood vessels, and that granulocyte colony-stimulating factor (G-CSF) mobilizes progenitors in the BMCs to the peripheral blood, while macrophage colony-stimulating factor (M-CSF) augments the production of monocytes. We examined whether M-CSF augments the differentiation of BMCs into endothelial cells of blood vessels using a hindlimb-ischemic model. Either G-CSF or M-CSF, or both, was administered to the hindlimb-ischemic mice for 3 days. Both M-CSF and G-CSF augmented the differentiation of BMCs into endothelial cells of blood vessels through vascular endothelial cell growth factor (VEGF), resulting in early recovery of blood flow in the ischemic limbs.
Minamino
Keizo
K
First Department of Pathology, Kansai Medical University, Moriguchi, Osaka 570-8507, Japan.
Adachi
Yasushi
Y
Okigaki
Mitsuhiko
M
Ito
Hidefumi
H
Togawa
Yoshimi
Y
Fujita
Kengo
K
Tomita
Minoru
M
Suzuki
Yasuhiro
Y
Zhang
Yuming
Y
Iwasaki
Masayoshi
M
Nakano
Keiji
K
Koike
Yasushi
Y
Matsubara
Hiroaki
H
Iwasaka
Toshiji
T
Matsumura
Miyo
M
Ikehara
Susumu
S
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004727:Endothelium / Q000737:chemistry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006183:H-2 Antigens / Q000032:analysis; D006614:Hindlimb / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D007511:Ischemia / Q000503:physiopathology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000098:blood supply; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D012039:Regional Blood Flow / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D006183:H-2 Antigens; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D016179:Granulocyte Colony-Stimulating Factor; D049452:Green Fluorescent Proteins; D016173:Macrophage Colony-Stimulating Factor