Long-term bone marrow culture data are the most powerful predictor of peripheral blood progenitor cell mobilization in healthy donors.
OBJECTIVE
There is wide interindividual variation in progenitor cell mobilization. The present study was aimed to analyze steady state hematopoiesis in healthy donors and its influence on hematopoietic progenitor cell (HPC) mobilization.


METHODS
Bone marrow (BM) was aspirated from 72 healthy donors prior to administration of recombinant human granulocyte colony-stimulating factor (G-CSF). Analyses of CD34+ cells and semisolid cultures as well as long-term cultures were performed from BM or leukapheresis products.


RESULTS
Male donors showed a higher number of BFU-E (p=0.007) and committed progenitors (p=0.05), a better stromal layer (p=0.02), and higher long-term bone marrow culture (LT-BMC) counts (p<0.05) when compared to those in female donors. When correlating the culture pattern of the BM with the data from the leukapheresis products, we observed that the number of the immature progenitors in BM correlated significantly with both the number of CD34 + cells and CFU-GM in the first leukapheresis. Univariate analysis revealed that the following variables had a beneficial impact on the number of CD34+ cells: male sex, body weight >73 Kg, G-CSF schedule and results of LT-BMC, although in the multivariate analysis only the number of CFU-GM obtained after LT-BMC showed a significant influence (p<0.001).


CONCLUSIONS
These results confirm the interindividual variation in HPC mobilization among healthy subjects, with LT-BMC counts being the most reliable predictor, expressing the behavior of the immature progenitors and their relationship with the microenvironment.
López-Holgado
Natalia
N
Department of Hematology, Hospital Clínico Universitario de Salamanca, Salamanca, Spain.
Pata
Carmen
C
Villarón
Eva
E
Sánchez-Guijo
Fermín
F
Alberca
Mercedes
M
Martín
Alejandro
A
Corral
Mercedes
M
Sánchez-Abarca
Ignacio
I
Pérez-Simón
Josè Antonio
JA
San Miguel
Jesús Fernando
JF
del Cañizo
Maria Consuelo
MC
D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002648:Child; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006410:Hematopoiesis; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods / Q000592:standards*; D006801:Humans; D007937:Leukapheresis / Q000592:standards; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D011446:Prospective Studies; D014019:Tissue Donors
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor