Combining sugar biosynthesis genes for the generation of L- and D-amicetose and formation of two novel antitumor tetracenomycins.
L- and D-stereoisomers of amicetose were generated by combining sugar biosynthesis genes from four different antibiotic gene clusters and both sugars were transferred to the elloramycin aglycone by the sugar flexible ElmGT glycosyltransferase.
Pérez
María
M
Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006, Oviedo, Spain.
Lombó
Felipe
F
Zhu
Lili
L
Gibson
Miranda
M
Braña
Alfredo F
AF
Rohr
Jurgen
J
Salas
José A
JA
Méndez
Carmen
C
D003001:Cloning, Molecular; D004798:Enzymes / Q000235:genetics; D006601:Hexoses / Q000096:biosynthesis* / Q000235:genetics*; D015394:Molecular Structure; D009279:Naphthacenes / Q000737:chemistry* / Q000145:classification / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D013237:Stereoisomerism
D004798:Enzymes; D006601:Hexoses; D009279:Naphthacenes; C080889:5838 DNI; C006487:amicetose