title: Antibabesial activity of protoberberine alkaloids and 20-hydroxyecdysone from Arcangelisia flava against Babesia gibsoni in culture.
abstract: Bioassay-guided fractionation of the boiled extract from the stems of Arcangelisia flava led to the isolation of palmatine (1), berberine (2), jatrorrhizine (3), dihydroberberine (4) and 20-hydroxyecdysone (5). The chemical structures of these compounds were elucidated on the basis of their chemical and spectral evidence. The isolated compounds were evaluated for their growth inhibiting effects on Babesia gibsoni in culture for a week. Compounds (1-4) showed significant inhibitions at concentrations from 100 to 1.0 microg/ml, while compound 5 at a concentration of 100 microg/ml, only.
authors.affiliation: Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.lastname: Subeki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.forename: Kosaku
authors.initials: K
authors.lastname: Takahashi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamato
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Maede
authors.forename: Ken
authors.initials: K
authors.lastname: Katakura
authors.forename: Mamoru
authors.initials: M
authors.lastname: Suzuki
authors.lastname: Trimurningsih
authors.lastname: Chairul
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Yoshihara
mesh_terms: D000818:Animals; D001403:Babesia / Q000166:cytology / Q000187:drug effects*; D001600:Berberine Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004441:Ecdysterone / Q000737:chemistry / Q000494:pharmacology*; D029583:Menispermaceae / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001600:Berberine Alkaloids; D004441:Ecdysterone