title: Dolomite supplementation improves bone metabolism through modulation of calcium-regulating hormone secretion in ovariectomized rats.
abstract: Dolomite, a mineral composed of calcium magnesium carbonate (CaMg (CO3)2), is used as a food supplement that supplies calcium and magnesium. However, the effect of magnesium supplementation on bone metabolism in patients with osteoporosis is a matter of controversy. We examined the effects of daily supplementation with dolomite on calcium metabolism in ovariectomized (OVX) rats. Dolomite was administered daily to OVX rats for 9 weeks. The same amount of magnesium chloride as that supplied by the dolomite was given to OVX rats as a positive control. Histological examination revealed that ovariectomy decreased trabecular bone and increased adipose tissues in the femoral metaphysis. Dolomite or magnesium supplementation failed to improve these bone histological features. Calcium content in the femora was decreased in OVX rats. Neither calcium nor magnesium content in the femora in OVX rats was significantly increased by dolomite or magnesium administration. Urinary deoxypyridinoline excretion was significantly increased in OVX rats, and was not affected by the magnesium supplementation. Serum concentrations of magnesium were increased, and those of calcium were decreased, in OVX rats supplemented with dolomite or magnesium. However, there was a tendency toward decreased parathyroid hormone secretion and increased calcitonin secretion in OVX rats supplemented with dolomite or magnesium. Serum 1,25-dihydroxyvitamin D(3) and osteocalcin levels were significantly increased in the supplemented OVX rats. These results suggest that increased magnesium intake improves calcium metabolism in favor of increasing bone formation, through the modulation of calcium-regulating hormone secretion.
authors.affiliation: Division of Hard Tissue Research, Institute for Oral Science, Matsumoto Dental University, 1780 Gobara, Hiro-oka, Shiojiri, 399-0781, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Mizoguchi
authors.forename: Sakae
authors.initials: S
authors.lastname: Nagasawa
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yagasaki
authors.forename: Michio
authors.initials: M
authors.lastname: Ito
mesh_terms: D000596:Amino Acids / Q000652:urine; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002117:Calcitriol / Q000097:blood; D002118:Calcium / Q000097:blood / Q000502:physiology*; D002119:Calcium Carbonate / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D005260:Female; D005269:Femur / Q000033:anatomy & histology / Q000737:chemistry; D008274:Magnesium / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D015675:Osteocalcin / Q000097:blood; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D010281:Parathyroid Hormone / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D010281:Parathyroid Hormone; D015675:Osteocalcin; C036020:deoxypyridinoline; D002117:Calcitriol; D002119:Calcium Carbonate; D008274:Magnesium; D002118:Calcium; C028042:calcium magnesium carbonate