PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.
PRDM1/Blimp-1 (in human and mouse, respectively) has a central role in determining and shaping the secretory arm of mature B-cell differentiation. In this study, a mouse monoclonal antibody that recognizes PRDM1 was used to detail its distribution in normal human lymphoid tissue and in lymphoid neoplasms that correspond to different stages of B-cell differentiation. PRDM1 was expressed in germinal centre blasts that co-express Pax5, CD19, CD20, and CD10, but not BCL6 or MTA-3. Pax5 was downregulated and full plasma cell morphology and phenotype were acquired by PRDM1+, nuclear cREL-, pre-plasma cells upon exit from the germinal centre. Activated extrafollicular B-cells (CD30+, Pax5+) were largely PRDM1-. PRDM1 was also absent in tissue histiocytes and the majority of resting T-cells and S-100+ antigen-presenting cells. PRDM1 and CD138 were expressed simultaneously in human lymphomas with plasma cell differentiation, but not in marginal zone lymphomas or chronic lymphocytic leukaemias. A minority of diffuse large B-cell lymphomas expressed PRDM1 and Hodgkin lymphomas were largely PRDM1-. Infiltrating T-cells in PRDM1- B-cell lymphomas expressed PRDM1. In conclusion, PRDM1 staining is a reliable and informative assay to define plasma cell commitment and differentiation in human normal and neoplastic B-cell lineages.
Cattoretti
Giorgio
G
Institute for Cancer Genetics, Columbia University, New York, NY 10032, USA. gc87@columbia.edu
Angelin-Duclos
Cristina
C
Shaknovich
Rita
R
Zhou
Huiping
H
Wang
Denong
D
Alobeid
Bachir
B
D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D018951:Antigens, CD20; D001402:B-Lymphocytes / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D002461:Cell Line, Transformed; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006689:Hodgkin Disease / Q000378:metabolism*; D006801:Humans; D017730:Ki-1 Antigen / Q000032:analysis; D016393:Lymphoma, B-Cell / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D015260:Neprilysin / Q000032:analysis; D051757:PAX5 Transcription Factor; D010950:Plasma Cells / Q000737:chemistry*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011509:Proteoglycans / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053668:Syndecan-1; D053667:Syndecans; D013601:T-Lymphocytes / Q000737:chemistry; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics
D018941:Antigens, CD19; D018951:Antigens, CD20; D015415:Biomarkers; D017252:CD3 Complex; D004268:DNA-Binding Proteins; D017730:Ki-1 Antigen; D008562:Membrane Glycoproteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; C497282:Pax5 protein, mouse; C513258:Prdm1 protein, mouse; D011509:Proteoglycans; D012333:RNA, Messenger; D012097:Repressor Proteins; C508241:SDC1 protein, human; C508242:Sdc1 protein, mouse; D053668:Syndecan-1; D053667:Syndecans; D014157:Transcription Factors; C087042:PRDM1 protein, human; D000074462:Positive Regulatory Domain I-Binding Factor 1; D015260:Neprilysin