title: Preoperative detection of laryngeal cancer in mucosal swabs by slide-based cytometry.
abstract: The aim of our study was to evaluate slide-based cytometry in screening for laryngeal cancer using swabs a minimally invasive approach. Laser scanning cytometry (LSC) was used for the multiparametric analysis of cells stained for cytokeratin and DNA to determine the DNA-index (DI) of the tumour cells. Histograms with DI < 0.95, 1.05 < DI < 1.9, and 2.1 < DI were defined as DNA aneuploid. After subsequent haemotoxylin-eosin (HE)-staining, single cells were re-localised and an analysis by conventional cytology was performed. Additionally, routine histopathology of parallel biopsies was obtained in all cases. Fifty one swabs from 49 lesions were analyzed. Seven and 17 swabs, were classified as insufficient for LSC and cytology, respectively. One and two benign lesions, were misclassified as malignant, respectively. Out of 34 malignant lesions, LSC detected 25 and cytology 14. LSC was superior to cytology in all of the statistical parameters tested. This pilot study demonstrates the validity of LSC for the preoperative detection of malignancy in laryngeal tumours using swabs.
authors.affiliation: Department of Ororhinolaryngology/Surgery, University of Bonn, D-53105 Bonn, Germany. gerstaoh@web.de
authors.forename: Andreas O H
authors.initials: AO
authors.lastname: Gerstner
authors.forename: Alexander
authors.initials: A
authors.lastname: Thiele
authors.forename: Attila
authors.initials: A
authors.lastname: Tárnok
authors.forename: Julia
authors.initials: J
authors.lastname: Machlitt
authors.forename: Jens
authors.initials: J
authors.lastname: Oeken
authors.forename: Andrea
authors.initials: A
authors.lastname: Tannapfel
authors.forename: Anette
authors.initials: A
authors.lastname: Weber
authors.forename: Friedrich
authors.initials: F
authors.lastname: Bootz
mesh_terms: D000465:Algorithms; D001707:Biopsy, Needle / Q000592:standards; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000601:surgery; D047569:Laser Scanning Cytometry / Q000592:standards; D010865:Pilot Projects; D011237:Predictive Value of Tests; D011300:Preoperative Care / Q000592:standards; D013048:Specimen Handling
chemical_list: D004273:DNA, Neoplasm