title: Human coxsackie adenovirus receptor (CAR) expression in transgenic mouse prostate tumors enhances adenoviral delivery of genes.
abstract: BACKGROUND
  Transgenic mice that recapitulate the progression of human diseases are potentially useful models for testing the effectiveness of new therapeutic strategies. Their use in pre-clinical testing of adenovirally-delivered gene therapies, however, is limited because of restricted cell surface expression of Coxsackie adenovirus receptor (CAR) in mice.
  
  
  METHODS
  To develop a more suitable transgenic mouse model for testing adenoviral-based gene therapies for prostate cancer, we generated prostate specific antigen/human CAR (PSA/hCAR) transgenic mice in which a chimeric enhancer/promoter sequence of the human PSA gene drives expression of a functional hCAR coding sequence.
  
  
  RESULTS
  Expression of an adenovirally-delivered luciferase reporter gene in prostate tumor cells in bigenic mice (PSA/hCAR + TRAMP) was enhanced compared to the level in tumor cells lacking the PSA/hCAR transgene.
  
  
  CONCLUSIONS
  Breeding PSA/hCAR mice to existing transgenic mouse models for prostate cancer (e.g., TRAMP) results in improved mouse models for testing adenovirally-delivered therapeutic genes.
authors.affiliation: Lankenau Institute for Medical Research, Wynnewood, Pennsylvania 19096, USA.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Bao
authors.forename: Weidan
authors.initials: W
authors.lastname: Peng
authors.forename: Amy
authors.initials: A
authors.lastname: Verbitsky
authors.forename: Jiping
authors.initials: J
authors.lastname: Chen
authors.forename: Lily
authors.initials: L
authors.lastname: Wu
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Rauen
authors.forename: Janet A
authors.initials: JA
authors.lastname: Sawicki
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D004195:Disease Models, Animal; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011471:Prostatic Neoplasms / Q000503:physiopathology / Q000628:therapy*; D011991:Receptors, Virus / Q000235:genetics*
chemical_list: C568771:CLMP protein, human; C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus