Cooperation of metal-ion fixation and target-site activation for efficient site-selective RNA scission.
Iminodiacetate-DNA conjugates and acridine-DNA conjugates were synthesized and combined for site-selective RNA hydrolysis by Lu(III). When these conjugates form a ternary complex with complementary RNA, the Lu(III)-iminodiacetate complex is placed near the target phosphodiester linkage of RNA which is in front of the acridine and is activated by noncovalent interactions. The site-selective hydrolysis by these combinations is several times as fast as that achieved by combining unmodified DNA (without iminodiacetate) and the acridine-DNA conjugate.
Kuzuya
Akinori
A
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo, 153-8904, Japan.
Shi
Yun
Y
Sasayama
Takuro
T
Komiyama
Makoto
M
D001483:Base Sequence; D001665:Binding Sites; D007477:Ions / Q000737:chemistry; D008187:Lutetium / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D007477:Ions; D008187:Lutetium; D012313:RNA