title: Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division.
abstract: Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states. In this study, we have used chromatin immunoprecipitation analysis to show that H3.3 is found mainly at the promoters of transcriptionally active genes. We also show that H3.3 combines with H3 acetylation and K4 methylation to form a stable mark that persists during mitosis. Our results suggest that H3.3 is deposited principally through the action of chromatin-remodelling complexes associated with transcriptional initiation, with deposition mediated by RNA polymerase II elongation having only a minor role.
authors.affiliation: Gene Regulation and Chromatin Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, London W12 0NN, UK.
authors.forename: Cheok-Man
authors.initials: CM
authors.lastname: Chow
authors.forename: Andrew
authors.initials: A
authors.lastname: Georgiou
authors.forename: Henrietta
authors.initials: H
authors.lastname: Szutorisz
authors.forename: Alexandra
authors.initials: A
authors.lastname: Maia e Silva
authors.forename: Ana
authors.initials: A
authors.lastname: Pombo
authors.forename: Isabel
authors.initials: I
authors.lastname: Barahona
authors.forename: Elise
authors.initials: E
authors.lastname: Dargelos
authors.forename: Claudia
authors.initials: C
authors.lastname: Canzonetta
authors.forename: Niall
authors.initials: N
authors.lastname: Dillon
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D042002:Chromatin Assembly and Disassembly / Q000235:genetics / Q000502:physiology; D047369:Chromatin Immunoprecipitation; D019084:Fluorescent Antibody Technique, Indirect; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009707:Nucleosomes / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology*
chemical_list: D006657:Histones; D009707:Nucleosomes