title: [Determination of deoxyribonucleic acid with nitro-tetrazolium chloride blue by resonance light-scattering technique].
abstract: Characteristics of the interaction of nitro-tetrazolium chloride blue and Deoxyribonucleic Acid (DNA) were studied in the of pH 1.8 buffer solution by Resonance Light-Scattering (RLS) technique. The effects of interference substances and the optimization of the procedure were investigated, and a new method for the determination of the DNA was established based on RLS. The linear range of the DNA is 0-7 mg x L(-1) with a correlation coefficient of 0.9958. The detection limit is 30.04 microg x L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.
authors.affiliation: Institute of Chemistry, Xiangtan University, Xiangtan 411105, China.
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Long
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Chen
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Cai
mesh_terms: D002712:Chlorides / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008027:Light; D057230:Limit of Detection; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts / Q000737:chemistry*
chemical_list: D002712:Chlorides; D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue; D004247:DNA