title: Candida digboiensis sp. nov., a novel anamorphic yeast species from an acidic tar sludge-contaminated oilfield.
abstract: Two strains (TERI-6(T) and TERI-7) of a novel yeast species were isolated from acidic tar sludge-contaminated soil samples collected from Digboi Refinery, Assam, India. These two yeast strains were morphologically, physiologically and phylogenetically identical to each other. No sexual reproduction was observed on corn meal, malt, Gorodkowa, YM or V8 agars. Physiologically, the novel isolates were most closely related to Candida blankii, but differed in eight physiological tests. The prominent differences were the ability of the isolates to assimilate melibiose and inulin and their inability to assimilate d-glucuronate, succinate and citrate. Phylogenetic analysis using the D1/D2 variable domain showed that the closest relative of these strains is C. blankii (2.8 % divergence). Other related species are Zygoascus hellenicus and Candida bituminiphila. The isolates differed from C. blankii by 11 base substitutions in the 18S rRNA gene sequence and by 58 base substitutions in the internal transcribed spacer sequences. The physiological, biochemical and molecular data support the contention that strains TERI-6(T) and TERI-7 represent a novel species, for which the name Candida digboiensis sp. nov. is proposed. The type strain is TERI-6(T) (=MTCC 4371(T)=CBS 9800(T)=JCM 12300(T)).
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector 39A, Chandigarh 160036, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Prasad
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector 39A, Chandigarh 160036, India.
authors.forename: S
authors.initials: S
authors.lastname: Mayilraj
authors.affiliation: TERI School of Advanced Studies, The Energy and Resources Institute (TERI), Darbari Seth Block, India Habitat Centre, Lodhi Road, New Delhi 110003, India.
authors.forename: Nitu
authors.initials: N
authors.lastname: Sood
authors.affiliation: TERI School of Advanced Studies, The Energy and Resources Institute (TERI), Darbari Seth Block, India Habitat Centre, Lodhi Road, New Delhi 110003, India.
authors.forename: Vijeyta
authors.initials: V
authors.lastname: Singh
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector 39A, Chandigarh 160036, India.
authors.forename: Kakoli
authors.initials: K
authors.lastname: Biswas
authors.affiliation: TERI, Darbari Seth Block, India Habitat Centre, Lodhi Road, New Delhi 110003, India.
authors.forename: Banwari
authors.initials: B
authors.lastname: Lal
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D019652:Extraction and Processing Industry; D006838:Hydrocarbons; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010578:Petroleum; D010641:Phenotype; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D006838:Hydrocarbons; D010578:Petroleum; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C065096:RNA, ribosomal, 26S; D012989:Soil Pollutants; C006647:asphalt