title: Translocation of nuclear factor-kappaB on corneal epithelial cells induced by ultraviolet B irradiation.
abstract: OBJECTIVE
  This study was performed to elucidate the role of nuclear factor-kappaB (NF-kappaB) in the death of corneal epithelial cells after ultraviolet (UV) irradiation.
  
  
  METHODS
  Simian virus 40-transfected human corneal epithelial cells (T-HCECs) were used in this study. Cell cultures were irradiated with a UVB (312 nm) source located 10 cm from the bottom of the slides for 10, 20, 30, or 40 s. Cytotoxicity was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Translocation of NF-kappaB was examined by immunocytochemistry using anti-NF-kappaB p65 antibody and electrophoretic mobility shift assay (EMSA). Sulfasalazine and SN-50, specific NF-kappaB inhibitors, were used to confirm the role of NF-kappaB by pretreating samples for 30 min before UV irradiation, after which cytotoxicity and NF-kappaB translocation were evaluated.
  
  
  RESULTS
  When T-HCECs were irradiated with UVB, translocation of NF-kappaB was observed with immunocytochemistry. These translocations peaked 2 h after UV irradiation during EMSA. When pretreated with sulfasalazine or SN-50, the translocation of NF-kappaB was blocked. Cellular death after UV irradiation was also markedly blocked by sulfasalazine.
  
  
  CONCLUSIONS
  These findings suggest that NF-kappaB plays an important role in cellular death after UV irradiation.
authors.affiliation: Department of Ophthalmology, Ilsan Paik Hospital, Inje University Medical College, 2240 Dae Wha-dong, Ilsan-ku, Koyang, Kyunggyi-do 411-706, Korea. dhlee@ilsanpaik.ac.kr
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Lee
authors.forename: Jung Kweon
authors.initials: JK
authors.lastname: Kim
authors.forename: Choun-Ki
authors.initials: CK
authors.lastname: Joo
mesh_terms: D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D002472:Cell Transformation, Viral; D024202:Electrophoretic Mobility Shift Assay; D019573:Epithelium, Corneal / Q000378:metabolism / Q000528:radiation effects*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D013539:Simian virus 40; D014178:Translocation, Genetic; D014466:Ultraviolet Rays
chemical_list: D016328:NF-kappa B