Culture of feline corneal epithelial cells and infection with feline herpesvirus-1 as an investigative tool.
OBJECTIVE
To isolate and characterize pure cultures of feline corneal epithelial cells and to assess the extent and nature of feline herpesvirus (FHV)-1 infection in these cells.


METHODS
Healthy eyes from 23 recently euthanatized cats.


METHODS
Stroma and epithelium of the rostral portion of the cornea were surgically isolated, and epithelial cells were detached from the stroma by enzymatic incubation. Epithelial cells were cultured in hormone-supplemented media. Cells were passaged, and cytokeratin expression was assessed. Cells were then infected with FHV-1, and cytopathic effects were determined.


RESULTS
Cell cultures were readily established from samples obtained from each eye and could be maintained through 6 passages. Cultured cells expressed cytokeratins 3 and 12 but not other cytokeratins. Infection with FHV-1 was rapid and caused widespread cytopathic effects.


CONCLUSIONS
Feline corneal cells cultured in vitro during multiple passages maintain consistent morphologic characteristics and intermediate filament expression. They are susceptible to infection with FHV-1 and may provide a useful in vitro model for investigation of ocular drugs.
Sandmeyer
Lynne S
LS
Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Keller
Charlotte B
CB
Bienzle
Dorothee
D
D000818:Animals; D002415:Cats; D018929:Cell Culture Techniques / Q000662:veterinary; D003588:Cytopathogenic Effect, Viral / Q000502:physiology; D019573:Epithelium, Corneal / Q000166:cytology* / Q000378:metabolism / Q000821:virology*; D006564:Herpesviridae / Q000502:physiology*; D007150:Immunohistochemistry / Q000662:veterinary; D053551:Keratin-12; D053558:Keratin-3; D007633:Keratins / Q000378:metabolism
C507494:KRT12 protein, human; C479360:KRT3 protein, human; D053551:Keratin-12; D053558:Keratin-3; D007633:Keratins