Vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage.
Agrobacterium tumefaciens was used to deliver the vhb gene into cabbage (Brassica oleracea var. Cabitata) cv. Xiaguang's parent line, 103. Using hypocotyls and cotyledon petioles as explants for infection, we obtained a transformation efficiency of 3-5% based on the number of transgenic shoots produced from the number of explants used for infection. Molecular analysis indicated that the vhb gene was stably integrated into the cabbage genome and that the vhb gene was expressed at the RNA level. Characterization of the vhb over-expressing transgenic plants revealed that transgenic seeds germinated faster than the wildtype controls. More importantly, the transgenic plants showed tolerance to a prolonged submergence treatment, suggesting that the vhb gene may provide an excellent tool for creation of submergence/flooding-tolerant cultivars of agriculturally important crops.
Li
Xian
X
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agri-biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China. lix17101@yahoo.com.cn
Peng
Ri-He
RH
Fan
Hui-Qin
HQ
Xiong
Ai-Sheng
AS
Yao
Quan-Hong
QH
Cheng
Zong-Ming
ZM
Li
Yi
Y
D000222:Adaptation, Physiological / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics* / Q000502:physiology; D002734:Chlorophyll / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D018525:Germination; D006454:Hemoglobins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012038:Regeneration; D012639:Seeds; D014170:Transformation, Genetic; D054793:Truncated Hemoglobins; D020589:Vitreoscilla / Q000235:genetics*
D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D002734:Chlorophyll