[Viability of the spleen in rats after ligation of the splenic vessels: effects of hyperbaric oxygen therapy].
OBJECTIVE
To investigate the effects of splenic artery and vein ligation and the influence of hyperbaric oxygen after the double vascular ligation on the viability of spleen tissue.


METHODS
Sixty nine adult male Wistar rats (285-375 g) were randomly separated in three groups: group 1, four rats, sham operated, group 2, 34 rats, submitted to simultaneous splenic artery and vein ligation and group 3, 31 rats, submitted to hyperbaric oxygen during 11 days, after double vascular ligation. All animals were killed on day 12 after surgery. The spleen was removed and paraffin embedded for microscopic examination.


RESULTS
In the groups submitted to vascular ligation, the spleen was normal in 8.82% of rats not treated with hyperbaric oxygen and in 45.16% of rats that received hyperbaric oxygen after vascular ligation (p=0.01). In the spleens with white infarct, the mass of preserved splenic tissue in relation to the total body mass did not differ between the groups treated or not with hyperbaric oxygen. The preserved splenic tissue had normal histology in both groups. The healing process was more accelerated in the group of rats treated with hyperbaric oxygen.


CONCLUSIONS
Results demonstrate that exposure to hyperbaric oxygen increased the frequency of total spleen mass preservation after simultaneous ligation of the splenic artery and vein but did not alter the percentage of the spleen's viable area, however the healing process in necrotic areas was accelerated.
Paulo
Danilo Nagib Salomão
DN
Laboratório de Técnica Operatória e Cururgia Experimental da Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória, ES.
Kalil
Mitre
M
Grillo Junior
Luiz Sérgio Pereira
LS
Borges
Erick Barcelos
EB
Cintra
Luiz Cálice
LC
Pereira
Fausto Edmundo Lima
FE
da Silva
Alcino Lázaro
AL
D000818:Animals; D006931:Hyperbaric Oxygenation; D008026:Ligation / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000098:blood supply* / Q000473:pathology; D013157:Splenic Artery; D013159:Splenic Infarction / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D013162:Splenic Vein