Liquid-liquid extraction of commercial glucose oxidase by reversed micelles.
Aim of this work was to establish the optimum operating conditions for the extraction and recovery by cationic reversed micelles of commercial glucose oxidase (GOX) from Aspergillus niger, in view of possible application to raw cell homogenates. The influence of pH, temperature, electric conductivity and solvent/co-solvents ratio on the extraction was investigated by a fractional factorial design of 2(3-1) type, conjugated with a mixture experimental design, using the residual enzyme activity to evaluate the results. The best conditions for GOX extraction were ensured using isooctane as solvent and hexanol and butanol as co-solvents at 76/6/18 volume ratio, pH 6.0, 0.2 M cetyl trimethylammonium bromide (CTAB) as cationic surfactant, and electrical conductivity (kappa) of 4.8 mS cm-1. The highest yield of GOX activity recovery (about 90%) was in fair accordance with the value predicted by the model.
Ferreira
Luis Fernando Peffi
LF
Biotechnology Interunit Post-graduation Program IPT/Butantã/USP, P.O. Box 66083, São Paulo, SP, Brazil.
Taqueda
Maria Elena
ME
Vitolo
Michele
M
Converti
Attilio
A
Pessoa
Adalberto
A
D001234:Aspergillus niger / Q000201:enzymology*; D002498:Centrifugation / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D003102:Colloids / Q000737:chemistry* / Q000302:isolation & purification*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003198:Computer Simulation; D005949:Glucose Oxidase / Q000302:isolation & purification*; D008823:Micelles; D008956:Models, Chemical; D012996:Solutions
D003102:Colloids; D008823:Micelles; D012996:Solutions; D005949:Glucose Oxidase