title: The effects of cage design on airborne allergens and endotoxin in animal rooms: high-volume measurements with an ion-charging device.
abstract: Respiratory symptoms related to both endotoxins and animal allergens continue to be an important cause of occupational disease for animal technicians and scientists working with rodents. Better sampling methods for airborne allergens and endotoxin are needed to help standardize compliance with federal occupational health regulations. Using an ion-charging device, we sampled 20 mouse rooms and four rat rooms at the University of Virginia, along with 43 domestic living rooms in houses in the Charlottesville area with at least one cat or dog. The use of filter tops on cages corresponds to a 50-fold reduction in mean levels of both airborne allergens (P < 0.001) and endotoxin (P < 0.001). The use of vented cages with filtered exhaust ports was associated with additional reductions. However, the mean airborne endotoxin level in all rooms using filter tops without a filtered exhaust port on the cages was significantly lower (P = 0.003) than the level in domestic living rooms. Our results for maximum airborne allergens or endotoxin are comparable with previous reports. However, the sensitivity of the technique allows an accurate assessment of low-level exposure, which makes it possible to evaluate the effect of cage designs. In addition, this approach allows direct comparison with results for airborne allergen and endotoxin in domestic homes. The results could allow a more consistent approach to the application of occupational health guidelines.
authors.affiliation: Department of Internal Medicine, University of Virginia, Charlottesville, Virginia 22908, USA.
authors.forename: James
authors.initials: J
authors.lastname: Platts-Mills
authors.forename: Natalie
authors.initials: N
authors.lastname: Custis
authors.forename: Alice
authors.initials: A
authors.lastname: Kenney
authors.forename: Amy
authors.initials: A
authors.lastname: Tsay
authors.forename: Martin
authors.initials: M
authors.lastname: Chapman
authors.forename: Sanford
authors.initials: S
authors.lastname: Feldman
authors.forename: Thomas
authors.initials: T
authors.lastname: Platts-Mills
mesh_terms: D016902:Air Pollution, Indoor / Q000032:analysis*; D000485:Allergens / Q000032:analysis*; D000818:Animals; D000830:Animals, Laboratory; D004731:Endotoxins / Q000032:analysis*; D004784:Environmental Monitoring / Q000295:instrumentation*; D006799:Housing, Animal; D007756:Laboratory Animal Science / Q000379:methods*; D051379:Mice; D016272:Occupational Health / Q000331:legislation & jurisprudence; D051381:Rats; D014768:Virginia
chemical_list: D000485:Allergens; D004731:Endotoxins