title: Sequence, conservation, and quantitative expression of rainbow trout Myf5.
abstract: The success of rainbow trout as an aquaculture species is dependent on the ability to produce fish with large amounts of high-quality lean muscle. It is therefore important to understand not only the best conditions under which to raise the fish but also the molecular control of muscle growth. Vertebrate muscle growth is initiated by the specification of myogenic precursor cells into myoblasts. The myoblasts proliferate and fuse to form multinucleated myotubes, which mature into myofibers. A family of basic helix-loop-helix (bHLH) transcription factors, the Myogenic Regulatory Factors (MRFs), controls these events. In trout, two MRF-encoding genes, TMyoD (of which there are two) and Tmyogenin, have been identified. However, the primary MRF-encoding Myf5 is not yet sequenced. Here, using degenerate PCR and 5' and 3' RACE, the cDNA sequence of trout Myf5 (TMyf5) is identified. Translation of the cDNA reveals that TMyf5 is a bHLH protein with homology to Myf5 and MRFs in other organisms. It is expressed mainly in red and white muscle, suggesting that it shares functional homology to Myf5 in other species. The molecular control of muscle growth has been well-characterized in mammals, but there are differences in the growth of fish muscle, highlighting the need for characterization of MRFs in fish species, particularly those in which understanding muscle growth will have a positive impact on the economic potential of the species.
authors.affiliation: USDA-ARS, Hagerman Fish Culture Experiment Station, 3059F National Fish Hatchery Rd., Hagerman, ID 83332, USA.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Johansen
authors.forename: Ken
authors.initials: K
authors.lastname: Overturf
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000737:chemistry; D051787:Myogenic Regulatory Factor 5; D017686:Oncorhynchus mykiss / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D051787:Myogenic Regulatory Factor 5; D012333:RNA, Messenger; D015534:Trans-Activators