title: Detection of human metapneumovirus RNA sequences in nasopharyngeal aspirates of young French children with acute bronchiolitis by real-time reverse transcriptase PCR and phylogenetic analysis.
abstract: Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, after respiratory syncytial virus and rhinovirus (35 [37%] and 21 [22%] of 94 cases, respectively). Phylogenetic analysis of F-gene sequences demonstrated the cocirculation of distinct HMPV genotypes during this study. These findings highlight the need to implement a rapid HMPV RT-PCR detection assay for the clinical diagnosis of respiratory infections in pediatric patients with bronchiolitis.
authors.affiliation: Laboratoire de Virologie, Service de Microbiologie, Hôpital Robert Debré, Avenue du Général Koenig, 51092 REIMS Cedex, France.
authors.forename: Maude
authors.initials: M
authors.lastname: Bouscambert-Duchamp
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
authors.forename: Aurélien
authors.initials: A
authors.lastname: Trompette
authors.forename: Hélène
authors.initials: H
authors.lastname: Moret
authors.forename: Jacques
authors.initials: J
authors.lastname: Motte
authors.forename: Laurent
authors.initials: L
authors.lastname: Andréoletti
mesh_terms: D000208:Acute Disease; D001483:Base Sequence; D001988:Bronchiolitis / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012621:Seasons
chemical_list: D012367:RNA, Viral