Efficacy of H5 influenza vaccines produced by reverse genetics in a lethal mouse model.
We studied the efficacy, in mice, of 2 H5 influenza vaccine viruses produced by reverse genetics. Mice were immunized with inactivated viruses and then inoculated with a human H5N1 1997 or 2003 virus or an avian H5N1 2001 virus. Vaccine viruses that we tested raised high levels of hemagglutination-inhibiting (1:160-1:1280) and virus-neutralizing (1:900-1:1900) antibodies on day 21 after a single dose of vaccine and decreased or prevented virus replication in mouse lungs; 54.5%-100% of immunized mice survived, whereas all control mice died. Protection was achieved despite antigenic differences and incomplete matching of the vaccine strain and the challenge virus. Therefore, high levels of cross-protection are predicted in the mouse model.
Lipatov
Aleksandr S
AS
Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-2794, USA.
Webby
Richard J
RJ
Govorkova
Elena A
EA
Krauss
Scott
S
Webster
Robert G
RG
D000818:Animals; D003429:Cross Reactions / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D009975:Orthomyxoviridae / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology* / Q000401:mortality / Q000821:virology*; D016896:Treatment Outcome; D014611:Vaccination; D014779:Virus Replication
D007252:Influenza Vaccines