title: Effects of a laminin peptide (YIGSR) immobilized on crab-tendon chitosan tubes on nerve regeneration.
abstract: Thiolated and nonthiolated hydroxyapatite-coated crab-tendon chitosan (t-chitosan/HAp-SH and t-chitosan/HAp, respectively) tubes, both alone and conjugated with CDPGYIGSR (YIGSR) peptide, were compared, in order to determine their biocompatibility and efficacy as nerve conduits. YIGSR peptide was adsorbed on the t-chitosan/HAp (HAp) tubes, and covalently bound on the t-chitosan/HAp-SH (HAp-SH) tubes (Y/HAp and Y/HAp-SH tubes, respectively). HAp, HAp-SH, Y/HAp, or Y/HAp-SH tubes measuring 15 mm were bridge grafted into the sciatic nerve of SD rats. Grafting of 15-mm-long Type I atelocollagen tubes and isografting of sciatic nerves were also carried out (N = 6 in each group). After 12 weeks, evoked muscle action potentials were recorded to calculate the terminal latency quotient. Histological observation and analysis of myelinated axons were also carried out. Nerve-tissue regeneration did not occur directly on the tubes' surfaces in the YIGSR peptide-unconjugated groups. Transplantation of YIGSR-conjugated tubes, however, gave rise to regenerated nerve tissue attached to thin layers of epineurium-like structure formed on the inner-tube surface. Histological and electrophysiological analyses suggested that although thiolation retards nerve-tissue regeneration, adsorbed YIGSR, and, to a lesser extent, peptide that had been covalently bound onto the tube surfaces, enhance nerve regeneration, promoting sprouting from the proximal nerve stump and bridging of regenerated axons throughout the tube.
authors.affiliation: Division of Molecular Tissue Engineering, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-ku, Tokyo 113-8510, Japan. itoso.gene@cmn.tmd.ac.jp
authors.forename: Soichiro
authors.initials: S
authors.lastname: Itoh
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsuda
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ichinose
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shinomiya
authors.forename: Junzo
authors.initials: J
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003386:Brachyura; D048271:Chitosan; D017886:Durapatite / Q000494:pharmacology; D007797:Laminin / Q000737:chemistry; D008422:Materials Testing; D008854:Microscopy, Electron; D009416:Nerve Regeneration / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology*; D019736:Prostheses and Implants; D051381:Rats; D013438:Sulfhydryl Compounds / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D007797:Laminin; D009842:Oligopeptides; D013438:Sulfhydryl Compounds; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; D048271:Chitosan; D017886:Durapatite