title: [Does colorectal cancer clinical advancement and surgical treatment affect platelet factor 4 concentration].
abstract: The aim of the present study was to determine platelet factor 4 (PF 4) concentration, as a marker of platelet activation, in patients with colorectal cancer (CRC), depending on the clinical advancement of carcinoma and surgical treatment.
  
  
  METHODS
  We investigated 21 patients with CRC before the surgery (A0) and three days (A1) and twelve days (A2) after the surgery. The patients were divided into three groups, according the clinical advancement--I, II, III (Hutter's classification). The control group (C) consisted 20 healthy subjects. PF 4 concentration was determined using the immunoenzymatic method (ELISA).
  
  
  RESULTS
  Patients with CRC before the surgery had a statistically significantly increased PF 4 concentration versus the control group (p<0.001). The highest PF 4 concentration was observed in patients without metastases (I degree advanced disease). Three days after the surgery PF 4 concentration decreased slightly in patients with III degree advanced disease. Twelve days after surgery (A2) it was again observed an increase of PF 4 concentration in patients with II and III stage of advancement.
  
  
  CONCLUSIONS
  Results of the study confirm that platelets are involved in development of cancer and indicate significantly that surgical treatment applied in this patients affects platelet activation and morphological parameters. The lower PF 4 concentration in patients with CRC with metastases (II and III group) confirm that more activity platelets play an important role in the metastases.
authors.affiliation: Zakład Laboratoryjnej Diagnostyki Klinicznej, Akademii Medycznej w Białymstoku. vpiekarska@yahoo.com
authors.forename: Violetta
authors.initials: V
authors.lastname: Dymicka-Piekarska
authors.forename: Anna
authors.initials: A
authors.lastname: Butkiewicz
authors.forename: Halina
authors.initials: H
authors.lastname: Kemona
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gryko
authors.forename: Maria
authors.initials: M
authors.lastname: Mantur
mesh_terms: D015179:Colorectal Neoplasms / Q000097:blood / Q000473:pathology / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D015539:Platelet Activation / Q000502:physiology*; D010978:Platelet Factor 4 / Q000502:physiology*; D011182:Postoperative Care; D011300:Preoperative Care; D016896:Treatment Outcome
chemical_list: D010978:Platelet Factor 4