Gelatin blending improves the performance of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) films for biomedical application.
To improve the performance of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), gelatin was blended with PHBHHx at different ratios. With increasing gelatin content, the weight loss of gelatin/PHBHHx blend in simulated body fluid at 37 degrees C was accelerated. After 2 months, there was about 15% weight loss in PHBHHx blending with 30% gelatin. Scanning electron microscopy and X-ray diffraction results showed that gelatin blending increased the surface porosity and decreased the crystallinity, which may be responsible for the acceleration of the weight loss. Second harmonic generation results indicated that 10% gelatin blending had less disruption to PHBHHx spatial structure, resulting in better tensile mechanical properties. At the same time, increased surface porosity and decreased crystallinity caused by gelatin incorporation may be beneficial for cell growth compared with pure PHBHHx. All these indicated that gelatin incorporation may improve the performances of PHBHHx to meet the need of different situations during medical implantation.
Wang
Ya-Wu
YW
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
Wu
Qiong
Q
Chen
Guo-Qiang
GQ
D020155:3-Hydroxybutyric Acid / Q000737:chemistry* / Q000627:therapeutic use; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D001826:Body Fluids / Q000378:metabolism; D002208:Caproates / Q000737:chemistry* / Q000627:therapeutic use; D002460:Cell Line; D049109:Cell Proliferation; D003460:Crystallization; D005780:Gelatin / Q000737:chemistry* / Q000627:therapeutic use; D051379:Mice; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology; D016062:Porosity; D013718:Tensile Strength; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D005780:Gelatin; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates