Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores.
Germination of Bacillus subtilis spores via the GerA nutrient receptor was suppressed by GerAC lacking the diacylglycerylated cysteine essential for receptor function. Overexpression of the C protein of the GerB nutrient receptor also suppressed the function of both the GerA receptor and a variant GerB receptor, GerB*. These findings suggest that GerAC and GerBC interact with their respective A and B proteins in GerA or GerB receptors and that GerBC potentially interacts with GerAA-GerAB. However, GerAC did not appear to interact with GerBA-GerBB.
Igarashi
Takao
T
Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305, USA. setlow@nso2.uchc.edu
Setlow
Peter
P
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D004075:Diglycerides / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004075:Diglycerides; D008565:Membrane Proteins