title: Electrodeposited nonconducting polytyramine for the development of glucose biosensors.
abstract: Biosensors with the composition of carbon/Prussian blue/(glucose oxidase+glutaraldehyde+polytyramine) were constructed. Before tyramine monomers were electropolymerized, glucose oxidase and tyramine monomers were cross-linked with glutaraldehyde onto the surface of Prussian-blue-modified electrodes. The constructed biosensors produced highly reproducible and stable devices. The biosensors exhibited neglectable decrease in current response after 10 repeated uses or after 1 month of dry storage. The resultant biosensors had a linear range of 0.1-1 mM glucose and a detection limit of 0.05 mM. Since the following electrocatalytic process proceeds at a low electrode potential (ca. -0.3 V vs Ag/AgCl), ascorbate and uric acid do not produce observable interfering signal for the determination of glucose.
authors.affiliation: Lab of Bioanalysis and Biosensor, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. biosensors@zjnu.cn
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Miao
authors.forename: Jianrong
authors.initials: J
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D014439:Tyramine / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose; D014439:Tyramine