Low immunogenicity of in vitro-expanded human neural cells despite high MHC expression.
The ability to expand human neural precursor cells in vitro offers new possibilities for future cell therapies. However, concern over immunologically based rejection of in vitro-expanded human neural cells confounds their use as donor cells. Here, we demonstrate that the expression of human leukocyte antigen (HLA) class I and II molecules, but not the co-stimulatory proteins CD40, CD80 and CD86, substantially increase during expansion of neurospheres. Furthermore, peripheral lymphocytes were unresponsive when co-cultured with in vitro-expanded neural cells. Taken together, these results suggest a low immunogenicity of these cultured human neural cells despite HLA incompatibility and high HLA expression.
Odeberg
Jenny
J
Neurotec Department, Division of Experimental Geriatrics, Karolinska Institutet, Karolinska University Hospital, KFC, 4th floor, Novum, SE-141 86 Stockholm, Sweden.
Piao
Jing-Hua
JH
Samuelsson
Eva-Britt
EB
Falci
Scott
S
Akesson
Elisabet
E
D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D005434:Flow Cytometry / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008213:Lymphocyte Activation / Q000502:physiology; D008285:Major Histocompatibility Complex / Q000502:physiology*; D009474:Neurons / Q000276:immunology*; D011247:Pregnancy; D016548:Prosencephalon / Q000166:cytology* / Q000196:embryology; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors
D015703:Antigens, CD