title: Application of phage-displayed single chain antibodies in western blot.
abstract: A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Ding
authors.forename: Mei-Yun
authors.initials: MY
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D000936:Antigen-Antibody Complex / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007128:Immunoglobulin Fragments / Q000276:immunology* / Q000302:isolation & purification*; D051379:Mice; D019151:Peptide Library
chemical_list: D000936:Antigen-Antibody Complex; D007128:Immunoglobulin Fragments; D019151:Peptide Library