Gas holdup, foaming and oxygen transfer in a jet loop bioreactor with artificial foaming media and yeast culture.
A concentric draft tube jet loop bioreactor (10.5 m3) was used to study the influence of aerated liquid height (above the draft tube) on the amount of surfactant addition allowable without foaming. Sodium lauryl sulfate (SLS) and defatted soybean flour in tap water were used as model artificial media. The amount of surfactant required to develop foaming and the maximum gas holdup achieved prior to foaming were notably influenced by aerated liquid height. Decreasing the aerated liquid height from 1.50 to 0.05 m increased the amount of SLS allowed without foaming from 2.2 to 12.1 g, the gas holdup in the riser from 0.18 to 0.31 and the gas holdup in the downcomer from 0.12 to 0.25. Similar behavior was observed for soybean flour. Decreasing the aerated liquid height from 1.45 to 0.05 m increased the amount of soybean flour allowed without foaming from 822 to 3200 g, the gas holdup in the riser from 0.17 to 0.26 and the gas holdup in the downcomer from 0.10 to 0.19. Data from a representative continuous yeast culture are reported to show that operation at low aerated liquid heights (0.5 m) can also be used to produce a culture broth with large gas holdup and oxygen transfer but without foaming.
Flores-Cotera
Luis B
LB
Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, México, D.F. 07360, Mexico. lfcotera@cinvestav.mx
García-Salas
Sergio
S
D019149:Bioreactors / Q000382:microbiology*; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D005740:Gases / Q000737:chemistry / Q000378:metabolism; D044085:Microfluidics / Q000379:methods; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*
D003470:Culture Media; D005740:Gases; D010100:Oxygen