Stage-dependent strategies of host invasion in the egg-larval parasitoid Chelonus inanitus.
Chelonus inanitus (Braconidae) is a solitary egg-larval parasitoid which lays its eggs into eggs of Spodoptera littoralis (Noctuidae); the parasitoid larva then develops in the haemocoel of the host larva. Host embryonic development lasts approx. 3.5 days while parasitoid embryonic development lasts approx. 16 h. All stages of host eggs can be successfully parasitized, and we show here that either the parasitoid larva or the wasp assures that the larva eventually is located in the host's haemocoel. (1) When freshly laid eggs, up to almost 1-day-old, are parasitized, the parasitoid hatches while still in the yolk and enters the host either after waiting or immediately through the dorsal opening. (2) When 1-2-day-old eggs are parasitized, the host embryo has accomplished final dorsal closure and is covered by an embryonic cuticle when the parasitoid hatches; in this case the parasitoid larva bores with its moving abdominal tip into the host. (3) When 2.5-3.5-day-old eggs are parasitized, the wasp oviposits directly into the haemocoel of the host embryo; from day 2 to 2.5 the embryo is still very small and the wasps, after probing, often restrain from oviposition for a few hours.
Kaeslin
Martha
M
Institute of Cell Biology, University of Berne, Baltzerstrasse 4, CH-3012 Bern, Switzerland.
Wehrle
Irene
I
Grossniklaus-Bürgin
Christa
C
Wyler
Toni
T
Guggisberg
Ursula
U
Schittny
Johannes C
JC
Lanzrein
Beatrice
B
D000818:Animals; D006790:Host-Parasite Interactions; D007814:Larva / Q000469:parasitology / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010058:Oviposition / Q000502:physiology*; D010063:Ovum / Q000166:cytology / Q000469:parasitology; D018411:Spodoptera / Q000196:embryology / Q000469:parasitology* / Q000648:ultrastructure; D014863:Wasps / Q000196:embryology / Q000502:physiology* / Q000648:ultrastructure