Identifying relapsing fever Borrelia, Senegal.
We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers. This technique, based on single nucleotide polymorphisms of the 16S ribosomal RNA gene, was used to test blood samples from 7,750 patients, 33 of whom were diagnosed with spirochete infections. Borrelia crocidurae was the only species identified.
Brahim
Hamoud
H
Institut Pasteur, Dakar, Senegal.
Perrier-Gros-Claude
Jean David
JD
Postic
Danielle
D
Baranton
Guy
G
Jambou
Ronan
R
D000328:Adult; D001898:Borrelia / Q000145:classification / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012061:Relapsing Fever / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D012675:Senegal / Q000453:epidemiology; D013045:Species Specificity