title: Proteome analysis of maternal serum samples for trisomy 21 pregnancies using ProteinChip arrays and bioinformatics.
abstract: A surface-enhanced laser desorption/ionization time of flight (SELDI-TOF)-based ProteinChip System was used as a tool for rapid discovery and identification of protein patterns in serum that discriminate between trisomy 21 and unaffected pregnancies. We analyzed 24 serum samples from women carrying a trisomy 21 pregnancy and 32 with an unaffected pregnancy, ranging from 10.0 to 14.0 weeks of gestation. The resulting protein profiles were submitted to a clustering algorithm, a rule extraction, a rating, and a rule base construction step. For the generated combined rule base, the specificity and sensitivity for the prediction of a trisomy 21 pregnancy reach 97% and 91%, respectively.
authors.affiliation: Core Unit Chip Application (CUCA), Inst. of Human Genetics and Anthropology, Friedrich Schiller University, D-07740 Jena, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Busch
authors.forename: Susanne
authors.initials: S
authors.lastname: Michel
authors.forename: Constance
authors.initials: C
authors.lastname: Hoppe
authors.forename: Dominik
authors.initials: D
authors.lastname: Driesch
authors.forename: Uwe
authors.initials: U
authors.lastname: Claussen
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
mesh_terms: D019295:Computational Biology; D004314:Down Syndrome / Q000097:blood / Q000175:diagnosis*; D005260:Female; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D044967:Serum; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D020543:Proteome