The effects of in utero and lactational exposure to chloroform on postnatal growth and glucose tolerance in male Wistar rats.
Water chlorination results in the formation of trihalomethanes (THMs) including chloroform. In human stud-ies, fetal growth restriction has been associated with exposure to THMs during pregnancy and impaired fetal growth has been associated with an increased risk of type 2 diabetes. Therefore, the objective of this study was to determine the effect of in utero and lactational exposure to chloroform on birthweight and postnatal indicators of type 2 diabetes. Female Wistar rats were given chloroform (0 microg/L, 75 microg/L) in their drinking water for 2 wk prior to mating until parturition (in utero exposure only) or until weaning (in utero+lactational exposure). At postnatal d 1 (PND1) pups of dams exposed to chloroform had significantly higher serum glucose levels and lower insulin levels, but this effect was not due to b<FONT "Optima">-cell depletion in the neonatal pancreas. Glucose homeostasis in response to a glucose challenge was not changed by chloroform treatment. Chloroform exposure did not affect birthweight; however, offspring of dams exposed to chloroform had significantly impaired postnatal growth. Although fetal and neonatal exposure to chloroform did not elicit physiological changes associated with the onset of type 2 diabetes, there were physiological changes resulting in impaired postnatal growth.
Lim
Gareth E
GE
Reproductive Biology Division, Department of Obstetrics & Gynecology, RM HSC 3N52, McMaster University, Hamilton, Ontario, Canada, L8N 3Z5.
Stals
Sandra I
SI
Petrik
James J
JJ
Foster
Warren G
WG
Holloway
Alison C
AC
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D002725:Chloroform / Q000633:toxicity*; D005260:Female; D005951:Glucose Tolerance Test; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018811:Maternal Exposure; D008892:Milk / Q000737:chemistry; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D001786:Blood Glucose; D012997:Solvents; D014874:Water Pollutants, Chemical; D002725:Chloroform