[Direct identification of Ophiogon japonicus (Thunb.)Ker-Gawl. from its confusable varieties by second derivative FTIR spectroscopy combined with statistics].
In order to establish the theory and method for the identification of Ophiogon japonicus (Thunb.)Ker-Gawl. of traditional Chinese herbal medicines and its confusable varieties, second derivative FTIR spectroscopy was used combined with statistics. Samples were collected directly by Fourier Transform Infrared (FTIR) spectra with OMNI-Sampler. Then through converting FTIR spectra of the samples into second derivative spectra by derivative spectra software, Ophiogon japonicus (Thunb.)Ker-Gawl. could be identified from the confusable varieties with statistics. The result shows that the second derivative FTIR of Ophiogon japonicus (Thunb.)Ker-Gawl. and its confusable varieties are different, which differ greatly in 2 000-650 cm(-1) range in second derivative FTIR spectroscopy. The Ophiogon japonicus (Thunb.)Ker-Gawl. and its confusable varieties can be identified by identifying the inner layer parts of the cuticles of samples by second derivative FTIR spectroscopy with statistics directly, rapidly and accurately.
Cheng
Cun-gui
CG
College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
Sun
Cui-rong
CR
Pan
Yuan-jiang
YJ
D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D008516:Medicine, Chinese Traditional / Q000592:standards*; D011420:Propiolactone / Q000737:chemistry; D012984:Software; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods* / Q000592:standards; D013057:Spectrum Analysis
D004365:Drugs, Chinese Herbal; D011420:Propiolactone