title: Immunohistochemical study on distribution of mast cell phenotypes in human laryngeal mucosa: evidence for laryngeal type I allergy.
abstract: It is controversial whether or not type I allergic reactions can occur in the human laryngeal mucosa. To clarify this issue, we studied the distribution of mast cell phenotypes in the human laryngeal mucosa using the immunohistochemical staining method with antihuman tryptase and chymase antibodies. A large number of mast cells are present in the superficial layer of subepithelial connective tissue (SECT) of the epiglottis, arytenoid, and subglottis. Although mast cells containing both tryptase and chymase are predominant in the deep layer of the SECT, the majority of mast cells containing tryptase alone are located in both the epithelial layer and the superficial layer of the SECT. We conclude that the human laryngeal mucosa has the potential to induce type I allergic reaction.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Ishida
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Shigemichi
authors.initials: S
authors.lastname: Iwae
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Amatsu
mesh_terms: D001193:Arytenoid Cartilage / Q000378:metabolism; D053818:Chymases; D004825:Epiglottis / Q000378:metabolism; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D007830:Larynx / Q000276:immunology / Q000378:metabolism*; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D009061:Mouth Mucosa / Q000276:immunology / Q000378:metabolism*; D010641:Phenotype; D012697:Serine Endopeptidases / Q000378:metabolism; D053802:Tryptases
chemical_list: D012697:Serine Endopeptidases; D053818:Chymases; D053802:Tryptases