title: [Genetic association of apoE and apoCI gene polymorphisms with coronary heart disease].
abstract: OBJECTIVE
  To study the genetic association of apolipoprotein (apo) E and apoCI gene polymorphisms with coronary heart disease (CHD) in China.
  
  
  METHODS
  apoE genotypes were identified by multiplex amplification refractory mutation system (multi-ARMS) and the apoCI promoter polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 186 cases with CHD (age: 65.0 +/- 10.5 years) and 350 controls (age: 63.6 +/- 8.3 years). The haplotype frequencies were estimated.
  
  
  RESULTS
  The frequencies of apoE E4/3 genotype (26.9%) and epsilon4 (14.5%) in CHD group were significantly higher than that in the control group (12.6%, 7.0%), P <0.05. The significant difference was also found for the apoCI locus and the CHD group showed higher rate of both for the H2 allele and genotypes, carrying this allele. Estimation of the haplotype frequencies indicated that the association between the apoE-CI haplotype and CHD was significantly strong. The apoE-epsilon4/apoCI-H2 was estimated to be responsible for 9.86% of CHD.
  
  
  CONCLUSIONS
  When the subjects carrying both epsilon4 and H2 alleles, they would have higher risk of suffering from CHD than controls.
authors.affiliation: School of public Health, Wuhan University, Wuhan 430071, China.
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Guang-di
authors.initials: GD
authors.lastname: Zhou
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Tan
authors.forename: Ding-fen
authors.initials: DF
authors.lastname: Han
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001056:Apolipoproteins C / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D002681:China / Q000453:epidemiology; D003327:Coronary Disease / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
chemical_list: D001056:Apolipoproteins C; D001057:Apolipoproteins E