title: Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A.
abstract: The bovine parvovirus (BPV) hemagglutinates human erythrocytes by binding to glycophorin A (GPA). The purpose of this study was to determine which carbohydrate on GPA binds BPV. Treatment of GPA with alpha2,3,-6,-8 neuraminidase eliminated binding of BPV to GPA. Beta-elimination of O-linked sialic acids on GPA eliminated binding, while removal of N-linked carbohydrates using the N-glycosidase PNGase F failed to eliminate binding. Treatment of GPA with a neuraminidase which specifically cleaved alpha2,3 glycosidic bonds eliminated BPV binding and, following this treatment, virus binding to GPA was restored by reconstitution of alpha2,3-linked neuraminic acids. These results indicated the O-linked alpha2,3 neuraminic acids of GPA bind BPV.
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah 84602, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Blackburn
authors.forename: S E
authors.initials: SE
authors.lastname: Cline
authors.forename: J P
authors.initials: JP
authors.lastname: Hemming
authors.forename: F B
authors.initials: FB
authors.lastname: Johnson
mesh_terms: D000818:Animals; D002417:Cattle / Q000821:virology*; D002462:Cell Membrane / Q000821:virology*; D006021:Glycophorins / Q000378:metabolism* / Q000502:physiology; D006384:Hemagglutination; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000502:physiology; D017992:Parvovirus / Q000378:metabolism*; D011991:Receptors, Virus / Q000502:physiology
chemical_list: D006021:Glycophorins; D011991:Receptors, Virus; D019158:N-Acetylneuraminic Acid