title: Gene expression profiling identifies a set of transcripts that are up-regulated inhuman testicular seminoma.
abstract: Seminoma constitutes one subtype of human testicular germ cell tumors and is uniformly composed of cells that are morphologically similar to the primordial germ cells and/or the cells in the carcinoma in situ. We performed a genome-wide exploration of the genes that are specifically up-regulated in seminoma by oligonucleotide-based microarray analysis. This revealed 106 genes that are significantly and consistently up-regulated in the seminomas compared to the adjacent normal tissues of the testes. The microarray data were validated by semi-quantitative RT-PCR analysis. Of the 106 genes, 42 mapped to a small number of specific chromosomal regions, namely, 1q21, 2p23, 6p21-22, 7p14-15, 12pll, 12p13, 12q13-14 and 22q12-13. This list of up-regulated genes may be useful in identifying the causative oncogene(s) and/or the origin of seminoma. Furthermore, immunohistochemical analysis revealed that the seminoma cells specifically expressed the six gene products that were selected randomly from the list. These proteins include CCND2 and DNMT3A and may be useful as molecular pathological markers of seminoma.
authors.affiliation: Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi 4-1, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yamada
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kohu
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Ishii
authors.forename: Shigeto
authors.initials: S
authors.lastname: Ishidoya
authors.forename: Shigeru
authors.initials: S
authors.lastname: Ishidoya
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: Satoru
authors.initials: S
authors.lastname: Kanto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukuzaki
authors.forename: Yutsu
authors.initials: Y
authors.lastname: Adachi
authors.forename: Mareyuki
authors.initials: M
authors.lastname: Endoh
authors.forename: Takuya
authors.initials: T
authors.lastname: Moriya
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Masanobu
authors.initials: M
authors.lastname: Satake
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Arai
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009857:Oncogenes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018239:Seminoma / Q000235:genetics*; D013736:Testicular Neoplasms / Q000235:genetics*; D015854:Up-Regulation