Nitric oxide-producing polyurethanes.
Thrombus formation and eventual intimal hyperplasia are the leading causes of small-diameter synthetic vascular graft failure. To combat these issues, we have incorporated a diazeniumdiolate-modified nitric oxide (NO)-producing peptide into a polyurethane to improve the thromboresistance of this biocompatible polymer. NO production by polyurethane films occurred for approximately 2 months under physiological conditions, and mechanical properties of the material were suitable for vascular graft applications. Platelet adhesion to NO-releasing polyurethane was dramatically decreased compared to control polyurethane. Furthermore, endothelial cell growth was stimulated in the presence of the NO-releasing polyurethane, while smooth muscle cell growth was greatly inhibited. The ability of this bioactive material to inhibit platelet adhesion and smooth muscle cell proliferation while encouraging endothelialization suggests that this NO-generating polyurethane may be suitable as a candidate material for small-diameter vascular grafts.
Jun
Ho-Wook
HW
Department of Bioengineering, Rice University, P.O. Box 1892, MS 142, Houston, Texas 77251-1892, USA.
Taite
Lakeshia J
LJ
West
Jennifer L
JL
D000818:Animals; D001011:Aorta; D001391:Azo Compounds; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000627:therapeutic use; D004730:Endothelium, Vascular / Q000166:cytology; D006083:Graft Occlusion, Vascular / Q000517:prevention & control*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D009569:Nitric Oxide / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D020030:Nitric Oxide Donors / Q000138:chemical synthesis / Q000494:pharmacology / Q000627:therapeutic use; D010973:Platelet Adhesiveness / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000209:etiology
D001391:Azo Compounds; D020099:Coated Materials, Biocompatible; D020030:Nitric Oxide Donors; D011140:Polyurethanes; C452539:diazeniumdiolate; D009569:Nitric Oxide