title: Alpha-crystallin: an ATP-independent complete molecular chaperone toward sorbitol dehydrogenase.
abstract: alpha-Crystallin, the major component of the vertebrate lens, is known to interact with proteins undergoing denaturation and to protect them from aggregation phenomena. Bovine lens sorbitol dehydrogenase (SDH) was previously shown to be completely protected by alpha-crystallin from thermally induced aggregation and inactivation. Here we report that alpha-crystallin, in the presence of the SDH pyridine cofactor NAD(H), can exert a remarkable chaperone action by favoring the recovery of the enzyme activity from chemically denaturated SDH up to 77%. Indeed, even in the absence of the cofactor, alpha-crystallin present at a ratio with SDH of 20:1 (w:w) allows a recovery of 35% of the enzyme activity. The effect of ATP in enhancing alpha-crystallin-promoted SDH renaturation appears to be both nonspecific and to not involve hydrolysis phenomena, thus confirming that the chaperone action of alpha-crystallin is not dependent on ATP as energy donor.
authors.affiliation: Dipartimento di Fisiologia e Biochimica, Sezione di Biochimica, Università di Pisa, Via S. Zeno, 51, 56100, Pisa, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Marini
authors.forename: R
authors.initials: R
authors.lastname: Moschini
authors.forename: A
authors.initials: A
authors.lastname: Del Corso
authors.forename: U
authors.initials: U
authors.lastname: Mura
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000502:physiology; D000818:Animals; D002417:Cattle; D004789:Enzyme Activation / Q000502:physiology; D019791:Guanidine / Q000494:pharmacology; D007064:L-Iditol 2-Dehydrogenase / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000737:chemistry* / Q000502:physiology; D011489:Protein Denaturation; D017510:Protein Folding; D038201:alpha-Crystallins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D038201:alpha-Crystallins; D009243:NAD; D000255:Adenosine Triphosphate; D007064:L-Iditol 2-Dehydrogenase; D019791:Guanidine