Identification of novel CBP interacting proteins in embryonic orofacial tissue.
cAMP response element-binding protein (CREB)-binding protein (CBP) plays an important role as a general co-integrator of multiple signaling pathways and interacts with a large number of transcription factors and co-factors, through its numerous protein-binding domains. To identify nuclear factors associated with CBP in developing orofacial tissue, a yeast two-hybrid screen of a cDNA library derived from orofacial tissue from gestational day 11 to 13 mouse embryos was conducted. Using the carboxy terminus (amino acid residues 1676-2441) of CBP as bait, several novel proteins that bind CBP were identified, including an Msx-interacting-zinc finger protein, CDC42 interaction protein 4/thyroid hormone receptor interactor 10, SH3-domain GRB2-like 1, CCR4-NOT transcription complex subunit 3, adaptor protein complex AP-1 beta1 subunit, eukaryotic translation initiation factor 2B subunit 1 (alpha), and cyclin G-associated kinase. Results of the yeast two-hybrid screen were confirmed by glutathione S-transferase pull-down assays. The identification of these proteins as novel CBP-binding partners allows exploration of new mechanisms by which CBP regulates and integrates diverse cell signaling pathways.
Yin
Xiaolong
X
Department of Molecular, Cellular and Craniofacial Biology, University of Louisville Birth Defects Center, ULSD Louisville, KY 40292, USA.
Warner
Dennis R
DR
Roberts
Emily A
EA
Pisano
M Michele
MM
Greene
Robert M
RM
D000818:Animals; D002478:Cells, Cultured; D003238:Connective Tissue / Q000196:embryology* / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D005145:Face / Q000196:embryology*; D008445:Maxillofacial Development / Q000502:physiology*; D051379:Mice; D009055:Mouth / Q000196:embryology / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014157:Transcription Factors / Q000378:metabolism*
D017362:Cyclic AMP Response Element-Binding Protein; D014157:Transcription Factors