Periprandial changes in growth hormone release in goldfish: role of somatostatin, ghrelin, and gastrin-releasing peptide.
In goldfish, growth hormone (GH) transiently rises 30 min after meals, returning to baseline at 1 h postmeal. Somatostatin (SRIF) is the major inhibitor of GH release. Three cDNAs encoding pre-pro-SRIF (PSS) have been previously cloned from goldfish brain: PSS-I, which encodes SRIF-14; PSS-II, which is potentially processed into gSRIF-28 that has [Glu(1),Tyr(7)(,)Gly(10)]SRIF-14 at the COOH terminus; and PSS-III, which encodes [Pro(2)]SRIF-14 at its COOH terminus. In goldfish, bombesin (BBS), mimicking the endogenous gastrin-releasing peptide (GRP), acutely suppresses food intake and also stimulates GH release. Ghrelin was recently characterized in goldfish as a GH secretagogue and an orexigen. In this paper, we studied the changes in SRIF mRNA levels during feeding and analyzed the influences of BBS and ghrelin peptides on forebrain PSS expression. The results showed a 60% reduction in PSS-II mRNA after meals, but no changes in the expression of PSS-I and PSS-III were found. Intraperitoneal injections of 100 ng/g body wt of BBS increased GH secretion and decreased PSS-I and PSS-II gene expression. Intraperitoneal injection of goldfish ghrelin (100 ng/g body wt) transiently increased the serum GH levels and increased PSS-I, while decreasing PSS-II mRNA levels. Ghrelin (50 ng/g body wt) blocked the effects of BBS (100 ng/g body wt) on PSS-I but not on PSS-II expression. Coadministration of BBS and ghrelin decreased only the PSS-II gene expression. We conclude that the interactions between BBS/GRP and ghrelin can account for the postprandial variations in serum GH levels and the forebrain expression of PSS-II. Furthermore, we demonstrate that intraperitoneal administration of BBS reduces the ghrelin expression levels in the gut. Thus the inhibition of production of ghrelin in the gut may contribute to the satiety effects of BBS/GRP peptides.
Canosa
Luis Fabián
LF
Department of Biological Sciences, CW405 Biological Sciences Bldg., University of Alberta, Edmonton, AB, Canada T6G 2E9.
Unniappan
Suraj
S
Peter
Richard Ector
RE
D000818:Animals; D001839:Bombesin / Q000008:administration & dosage / Q000494:pharmacology; D004347:Drug Interactions; D019886:Gastrin-Releasing Peptide / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D054439:Ghrelin; D006054:Goldfish / Q000378:metabolism / Q000502:physiology*; D013006:Growth Hormone / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007413:Intestinal Mucosa / Q000378:metabolism; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology; D036361:Peptide Hormones / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology*; D019518:Postprandial Period; D016548:Prosencephalon / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013004:Somatostatin / Q000235:genetics / Q000502:physiology*; D013997:Time Factors
D054439:Ghrelin; D010446:Peptide Fragments; D036361:Peptide Hormones; D011498:Protein Precursors; D012333:RNA, Messenger; D013004:Somatostatin; D019886:Gastrin-Releasing Peptide; D013006:Growth Hormone; D001839:Bombesin