title: Bone tissue induction, using a COLLOSS-filled titanium fibre mesh-scaffolding material.
abstract: Scaffold materials for bone tissue engineering often are supplemented with bone morphogenetic proteins (BMPs). In the current study we aimed to investigate COLLOSS, a bovine extracellular matrix product containing native BMPs. Hollow cylindrical implants were made, with a length of 10 mm, a 3 mm inner diameter, and a 5 mm outer diameter, from titanium fibre mesh. The central space of the tube was filled with 20 mg COLLOSS. Subsequently, these implants, as well as non-loaded controls, were implanted subcutaneously into the back of Wistar rats, with n=6 for all study groups. After implantation periods of 2, 8, and 12 weeks, tissue-covered implants were retrieved, and sections were made, perpendicular to the long axis of the tube. Histology showed, that all implants were surrounded by a thin fibrous tissue capsule. After 2 weeks of implantation, the COLLOSS material was reduced in size inside the loaded implants, but no bone-like tissue formation was evident. After 8 weeks, in two out of six loaded specimens, new-formed bone- and bone marrow-like tissues could be observed. After 12 weeks, this had increased to five out of six COLLOSS-loaded samples. The amount of bone-like tissue did not differ between 8 and 12 weeks, and on average occupied 15% of the central space of the tube. In the non-loaded control samples, only connective tissue ingrowth was observed. In conclusion, we can say that COLLOSS material loaded in a titanium fibre mesh tube, showed bone-inducing properties. The final efficacy of these osteo-inductive properties has to be confirmed in future large animal studies.
authors.affiliation: Department of Periodontology and Biomaterials, University Medical Center, P.O. Box 9101, THK 117 PB, 6500 HB Nijmegen, The Netherlands.
authors.forename: X Frank
authors.initials: XF
authors.lastname: Walboomers
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D020007:Implants, Experimental; D008422:Materials Testing; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; C487179:Colloss; D014025:Titanium