[Determination of germanium in A. arborescens by spectrophotometric method].
A new spectrophotometric method for the determination of germanium in A. arborescens was developed. The method was based on the fact that germanium forms a stable complex with phenylfluorone in the acidified solution with CTMAB as solubilization agent. The maximum absorption of association complex is at 530 nm, the linear range is 0.1-0.7 microg x mL(-1), the regression equation is A = 0.0817 + 0.8677c530, (microg x mL(-1)) the correlation coefficient r=0.9177, and the limit of detection is 0.65 microg x mL(-1). The leaves and stems of A. arborescens were determined by the proposed method. The total amount of germanium is 697.0-1219.5 ng x g(-1), and the content of organic Ge is 95.45%-98.99%. The method is suitable for the analysis of samples which contain both organic Ge and inorganic Ge.
Hui
Rui-hua
RH
Analytical and Testing Research Center, Anshan Normal University, Anshan 114005, China.
Hou
Dong-yan
DY
Guan
Chong-xin
CX
D005452:Fluoresceins / Q000737:chemistry; D005857:Germanium / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D005452:Fluoresceins; D004364:Pharmaceutical Preparations; D005857:Germanium; C010159:fluorone Black