title: Crystal structure of the N-terminal domain of the group B streptococcus alpha C protein.
abstract: Group B Streptococcus (GBS) is the leading cause of bacterial pneumonia, sepsis, and meningitis among neonates and an important cause of morbidity among pregnant women and immunocompromised adults. Invasive diseases due to GBS are attributed to the ability of the pathogen to translocate across human epithelial surfaces. The alpha C protein (ACP) has been identified as an invasin that plays a role in internalization and translocation of GBS across epithelial cells. The soluble N-terminal domain of ACP (NtACP) blocks the internalization of GBS. We determined the 1.86-A resolution crystal structure of NtACP comprising residues Ser(52) through Leu(225) of the full-length ACP. NtACP has two domains, an N-terminal beta-sandwich and a C-terminal three-helix bundle. Structural and topological alignments reveal that the beta-sandwich shares structural elements with the type III fibronectin fold (FnIII), but includes structural elaborations that make it unique. We have identified a potential integrin-binding motif consisting of Lys-Thr-Asp(146), Arg(110), and Asp(118). A similar arrangement of charged residues has been described in other invasins. ACP shows a heparin binding activity that requires NtACP. We propose a possible heparin-binding site, including one surface of the three-helix bundle, and nearby portions of the sandwich and repeat domains. We have validated this prediction using assays of the heparin binding and cell-adhesion properties of engineered fragments of ACP. This is the first crystal structure of a member of the highly conserved Gram-positive surface alpha-like protein family, and it will enable the internalization mechanism of GBS to be dissected at the atomic level.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Thierry C
authors.initials: TC
authors.lastname: Aupérin
authors.forename: Gilles R
authors.initials: GR
authors.lastname: Bolduc
authors.forename: Miriam J
authors.initials: MJ
authors.lastname: Baron
authors.forename: Annie
authors.initials: A
authors.lastname: Heroux
authors.forename: David J
authors.initials: DJ
authors.lastname: Filman
authors.forename: Lawrence C
authors.initials: LC
authors.lastname: Madoff
authors.forename: James M
authors.initials: JM
authors.lastname: Hogle
mesh_terms: D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002478:Cells, Cultured; D003460:Crystallization; D018360:Crystallography, X-Ray; D004847:Epithelial Cells / Q000201:enzymology / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D013292:Streptococcus agalactiae / Q000201:enzymology* / Q000235:genetics
chemical_list: D000954:Antigens, Surface; D001426:Bacterial Proteins; D010446:Peptide Fragments; C077961:alpha C protein, group B streptococci