The effect of cooling on the acetylcholine-induced current of identified Helix pomatia Br neuron.
The Br neuron of the snail Helix pomatia, involved in neuronal regulation of various homeostatic and adaptive mechanisms, represents an interesting model for studying effects of temperature changes on neuronal activity of poikilotherms. The acetylcholine (ACh) induces a transient, inward dose-dependent current in the identified Br neuron. In the work presented, we analyses the effects of cooling on the ACh-induced inward current. The amplitude of ACh-induced inward current was markedly decreased after cooling and the speed of the decay of ACh response was decreased. Sensitivity to cooling of Ach-activated current on the Br neuron is mediated by a mechanism that does not involve change in the apparent receptor affinity or the cooperativity of binding.
Nedeljkovic
Miodrag
M
Department of Neurophysiology, Institute for Biological Research, Belgrade University, Belgrade, Serbia and Montenegro.
Kartelija
Gordana
G
Radenovic
Lidija
L
Todorovic
Natasa
N
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D017952:Ganglia, Invertebrate / Q000187:drug effects / Q000502:physiology*; D006372:Helix, Snails / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D014403:Tubocurarine / Q000494:pharmacology
D000109:Acetylcholine; D014403:Tubocurarine