title: Compartmentalization of TCR repertoire alteration during rejection of an intrabrain xenograft.
abstract: Xenograft rejections of embryonic pig neural cells implanted into the adult rat striatum occurs within 3-4 weeks, following a dramatic T cell infiltration. Little is known about the cross-talk between the brain and peripheral lymphoid tissues which results in this recruitment and lymphocyte homing. To better characterize the dynamics of the T cell response against xenogeneic neural cells implanted into the brain parenchyma, we used both qualitative and quantitative methods to follow the alterations of the CDR3 length distribution (CDR3-LD) of the TCR (T cell receptor) beta chain in the transplanted striatum and compared this response to that observed in the deep cervical lymph nodes, spleen, and blood. Data showed that the T cell repertoire diversity was highly altered in the recipient brain during xenograft rejection. Comparison of the alterations of the CDR3-LD between several animals revealed a single public alteration in the Vbeta20 family, and many private alterations of the CDR3-LD which differed from one infiltrated brain to another. Alterations of the T cell repertoire were also observed in lymphocytes homed into the deep cervical lymph nodes. However, they differed from the alterations detected in the infiltrated brains. Conversely, no significant alteration of the CDR3-LD was detected in the spleen or in the blood. These data suggest that the deep cervical lymph nodes play an active role in the process of xenograft recognition or/and rejection. However, they also indicate that the fate of T cells homed in the brain and deep cervical lymph nodes differs.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, 44093 Nantes, Cedex 01, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Melchior
authors.forename: Véronique
authors.initials: V
authors.lastname: Nérrière-Daguin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Degauque
authors.forename: Sophie
authors.initials: S
authors.lastname: Brouard
authors.forename: Marina
authors.initials: M
authors.lastname: Guillet
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Soulillou
authors.forename: Philippe
authors.initials: P
authors.lastname: Brachet
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000378:metabolism; D003342:Corpus Striatum / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D004622:Embryo, Mammalian; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D011917:Rats, Inbred Lew; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014183:Transplantation, Heterologous
chemical_list: D022801:Complementarity Determining Regions; D011948:Receptors, Antigen, T-Cell