The role of the 132-160 region in prion protein conformational transitions.
The native conformation of host-encoded cellular prion protein (PrP(C)) is metastable. As a result of a post-translational event, PrP(C) can convert to the scrapie form (PrP(Sc)), which emerges as the essential constituent of infectious prions. Despite thorough research, the mechanism underlying this conformational transition remains unknown. However, several studies have highlighted the importance of the N-terminal region spanning residues 90-154 in PrP folding. In order to understand why PrP folds into two different conformational states exhibiting distinct secondary and tertiary structure, and to gain insight into the involvement of this particular region in PrP transconformation, we studied the pressure-induced unfolding/ refolding of recombinant Syrian hamster PrP expanding from residues 90-231, and compared it with heat unfolding. By using two intrinsic fluorescent variants of this protein (Y150W and F141W), conformational changes confined to the 132-160 segment were monitored. Multiple conformational states of the Trp variants, characterized by their spectroscopic properties (fluorescence and UV absorbance in the fourth derivative mode), were achieved by tuning the experimental conditions of pressure and temperature. Further insight into unexplored conformational states of the prion protein, likely to mimic the in vivo structural change, was obtained from pressure-assisted cold unfolding. Furthermore, salt-induced conformational changes suggested a structural stabilizing role of Tyr150 and Phe141 residues, slowing down the conversion to a beta-sheet form.
Torrent
Joan
J
INSERM U710, CC 105, Université de Montpellier 2, Place Eugène Bataillon, F-34095 Montpellier cédex 5, France.
Alvarez-Martinez
Maria Teresa
MT
Liautard
Jean-Pierre
JP
Balny
Claude
C
Lange
Reinhard
R
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D006224:Cricetinae; D005615:Freezing; D008647:Mesocricetus; D011328:Prions / Q000737:chemistry*; D011487:Protein Conformation / Q000187:drug effects; D017510:Protein Folding; D012965:Sodium Chloride / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D011328:Prions; D012965:Sodium Chloride