Photodynamic therapy using a novel photosensitizer, ATX-S10(Na): comparative effect with 5-aminolevulinic acid on squamous cell carcinoma cell line, SCC15, ultraviolet B-induced skin tumor, and phorbol ester-induced hyperproliferative skin.
Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) is available for the treatment of actinic keratosis (AK). Recently, we developed a new PDT photosensitizer, ATX-S10(Na), and have shown that ATX-S10(Na) PDT is effective for the treatment of various human skin diseases, such as squamous cell carcinoma, Bowen's disease, basal cell carcinoma, and psoriasis. In the present study, we compared the effects of ATX-S10(Na) PDT and ALA PDT on hyperproliferative skin induced by 12-O-tetradecanoylphorbol-13-acetate (TPA), on the squamous cell carcinoma cell line, SCC15, in vitro, and on UVB-induced skin tumors in vivo. TPA treatment induced epidermal acanthosis, which was more markedly suppressed by ATX-S10(Na) PDT than by ALA PDT. ATX-S10(Na) PDT more effectively eliminated UVB-induced AK and squamous cell carcinoma (SCC) than ALA PDT. Furthermore, both ATX-S10(Na) PDT and ALA PDT induced the death of SCC15 cells, and the effect of ATX-S10(Na) PDT was greater than that of ALA PDT. Our results indicate that ATX-S10(Na) PDT might be more effective than ALA PDT for the treatment of various skin diseases.
Takahashi
Hidetoshi
H
Department of Dermatology, Asahikawa Medical College, 2-1-1-1 Midorigaokahigashi, Asahikawa 078-8510, Japan. ht@asahikawa-med.ac.jp
Nakajima
Susumu
S
Sakata
Isao
I
Ishida-Yamamoto
Akemi
A
Iizuka
Hajime
H
D000622:Aminolevulinic Acid / Q000627:therapeutic use*; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000188:drug therapy; D018307:Neoplasms, Squamous Cell / Q000139:chemically induced / Q000188:drug therapy*; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000627:therapeutic use*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000188:drug therapy*; D013755:Tetradecanoylphorbol Acetate; D014466:Ultraviolet Rays
C117772:ATX S10Na(II); D011166:Porphyrins; D000622:Aminolevulinic Acid; D013755:Tetradecanoylphorbol Acetate