A straight-forward method of optimising protein solubility for NMR.
Maximising solubility is a key step in applying solution-state NMR to proteins. The 'microbatch' crystallisation screening method can be adapted to screen for protein solubility. In this approach, drops of test solutions are placed under paraffin oil in 96-well screening plates. This requires very small amounts of protein, is easy to set up and is readily automatable.
Howe
Peter W A
PW
Analytical Sciences, Syngenta, Jealott's Hill Research Centre, Bracknell, Berkshire, RG42 6EY, UK. peter.howe@syngenta.com
D029688:Brassica napus / Q000737:chemistry*; D003460:Crystallization; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010940:Plant Proteins / Q000737:chemistry*; D012995:Solubility
D010940:Plant Proteins