[Determination and pharmacokinetics of photosensitizer ZnPcS2P2 uptaken by K562 cells].
The di-sulfonated di-phthalimidomethyl phthalocyanine zinc (ZnPcS2P2) was an amphiphilic photosensitizer for photodynamic therapy of cancer. According to fluorescence analytical method, extracting efficiencies of 2.0% SDS (Wt%), 1.5% Triton X-100 (phi) and DMF for ZnPcS2P2 in K562 cells were compared. The results showed that 1.5% Triton X-100 was the most efficient reagent for the first time extracting. Using 1.5% Triton X-100 as extracting reagent, the kinetic curves of cellular uptake of ZnPcS2P2 as well as LDL-ZnPcS2P2 complex by K562 cells were determined. Compared with ZnPcS2P2, the maximum cellular uptake of LDL-ZnPcS2P2 by K562 was doubled, which indicated that LDL was an effective targeting delivery vehicle for ZnPcS2P2.
Xie
Bao-gang
BG
Department of Chemistry, Institute of Research on Functional Materials, Fuzhou University, Fuzhou 350002, China.
Huang
Hui-fang
HF
Huang
Jian-dong
JD
Chen
Nai-sheng
NS
Huang
Jin-ling
JL
D004247:DNA / Q000032:analysis; D016313:Genes, abl / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D020014:K562 Cells / Q000378:metabolism*; D017830:Octoxynol / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured
D007211:Indoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; C064328:zinc phthalocyanine sulfonate; C064329:zinc phthalocyanine disulfonate; D017830:Octoxynol; D004247:DNA