CD13/aminopeptidase N and murine cytomegalovirus infection.
CD13/aminopeptidase N is a membrane-bound metalloproteinase implicated in human cytomegalovirus (HCMV) infection and pathogenesis. Anti-CD13 antibodies can neutralize HCMV infectivity, and HCMV viremia after bone marrow transplantation induces anti-CD13 autoantibodies which correlate with development of chronic graft vs. host disease. We examined whether murine CD13/APN was similarly implicated in murine cytomegalovirus (MCMV) disease. MCMV infection did induce anti-CD13 antibodies in mice in a strain-specific manner. ICR and 129S mice developed high titers of anti-CD13 antibodies and anti-MCMV antibodies after MCMV infection, whereas CBA and CBAxC57BL/6 f1 hybrid mice produced antibodies against MCMV only. Unlike HCMV, no evidence was found for a correlation between host cell CD13/APN expression and infection, or for the presence of CD13/APN on MCMV particles, although APN inhibitors decreased MCMV plaque formation. Reproduction of CD13/APN autoantibody production in the murine system should make it possible to determine if these antibodies contribute to CMV pathogenesis.
Kasman
Laura M
LM
Department of Microbiology and Immunology, Medical University of South Carolina, BSB-201, PO Box 250504, 173 Ashley Avenue, Charleston, SC 29425, USA. kasmanl@musc.edu
D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis; D018826:CD13 Antigens / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000201:enzymology / Q000209:etiology / Q000276:immunology; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006566:Herpesviridae Infections / Q000201:enzymology* / Q000209:etiology / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D018146:Muromegalovirus; D009500:Neutralization Tests; D010618:Phenanthrolines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D013045:Species Specificity
D001323:Autoantibodies; D010618:Phenanthrolines; D011480:Protease Inhibitors; D004492:Edetic Acid; D018826:CD13 Antigens; C025205:1,10-phenanthroline