Scavenging of reactive oxygen species by novel indolin-2-one and indoline-2-thione derivatives.
The antioxidant behavior of a series of new synthesized substituted indoline-2-ones and indolin-2-thiones was investigated in this study using an oxygen radical absorbance capacity assay (ORAC(ROO*-) and 2,2'-azobis(2-amidino-propane) dihydrochloride (AAPH) as the radical generator; system generating superoxide anion radical, O2*- (18-crown-6/KO(2)/DMSO), and the Fenton-like reaction [Co(II) + H(2)O(2) --> Co(III) + HO(*) + HO(-)]. Measurements were done using fluorescence, chemiluminescence methods, and a deoxyribose assay based on the spectrophotometry method, respectively. The results obtained indicated that the examined indoline derivatives had effective activities as radical scavengers and may be considered as an effective source for combating oxidative damage.
Aboul-Enein
Hassan Y
HY
Bioanalytical and Drug Development Laboratory, Biological & Research Centre, P. O. Box 3354, Riyadh 11211, Saudi Arabia. enien@kfshrc.edu.sa
Kładna
Aleksandra
A
Kruk
Irena
I
Lichszteld
Krzysztof
K
Michalska
Teresa
T
Olgen
Sureyya
S
D000975:Antioxidants / Q000737:chemistry*; D002384:Catalysis; D003855:Deoxyribose / Q000032:analysis / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism*; D008163:Luminescent Measurements; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000378:metabolism; D013871:Thiones / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D007211:Indoles; D017382:Reactive Oxygen Species; D013871:Thiones; C456900:indolin-2-one; D013481:Superoxides; D017665:Hydroxyl Radical; D003855:Deoxyribose; C057812:indoline; D010100:Oxygen