Determination of ochratoxin A in wine by liquid chromatography tandem mass spectrometry after combined anion-exchange/reversed-phase clean-up.
Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus ochraceus and Penicillium verrucosum. It has been found and analysed in several foods and feeds. Owing to its toxicity and occurrence in food and feed, the European Community has issued directives and some countries have their own regulations for OTA contents in food, feed and beverages. This work describes a method for the determination of OTA in mulled and red wine. It is based on combined anion exchange/reversed-phase clean-up and was analysed by liquid chromatography coupled with tandem mass spectrometry (multiple reaction monitoring). The method was validated with natural contaminated and spiked wine samples with OTA contents from 1.34 to 3.48 microg kg(-1). Owing to its accuracy, good reproducibility and repeatability, this easy method is a good alternative to liquid chromatography-fluorescence detection methods.
Reinsch
M
M
Department of Analytical Chemistry, Federal Institute for Materials Research and Testing, Richard-Willstätter-Strasse 11, 12489 Berlin, Germany. martin.reinsch@bam.de
Töpfer
A
A
Lehmann
A
A
Nehls
I
I
D002853:Chromatography, Liquid / Q000379:methods*; D007474:Ion Exchange; D013058:Mass Spectrometry / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
D009793:Ochratoxins; C025589:ochratoxin A