title: YidC-dependent translocation of green fluorescence protein fused to the FliP cleavable signal peptide.
abstract: Escherichia coli FliP is a rare bacterial polytopic membrane protein synthesized with a cleavable, highly hydrophobic signal peptide. More hydrophilic Tat-dependent or Sec-dependent signal peptide is functionally capable of substituting for the FliP signal peptide, but a signal anchor of inner membrane protein fails to do so. To assess the intrinsic characteristics of the FliP signal peptide in mediating protein translocation, we fused it to green fluorescence protein and observed that the translocation of the chimera (FliPss-GFP) was dependent of Ffh, SecA, SecY and SecD. In addition, we showed for the first time the involvement of YidC in protein translocation across the inner membrane.
authors.affiliation: Laboratoire de Chimie Bactérienne, UPR9043, IBSM, CNRS, 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pradel
authors.forename: Alexandre
authors.initials: A
authors.lastname: Decorps
authors.forename: Changyun
authors.initials: C
authors.lastname: Ye
authors.forename: Claire-Lise
authors.initials: CL
authors.lastname: Santini
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Wu
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; C503156:FliP protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; C412443:YIDC protein, E coli; D049452:Green Fluorescent Proteins