[Detection of organic autotrophic particles by FCM (flow cytometry) in an oligotrophic lake].
Organic autotrophic particles were detected by flow cytometry (FCM) in an oligotrophic lake. Utilizing scattering signals and fluorescence of their natural pigments, different populations could be rapidly discriminated and enumerated. We found that they belonged to three populations. R1 were the smallest population, while R3 were the largest one. R1 contained phycoerythrin but lacked chlorophyll. On the contrary, R3 contained a large amount of chlorophyll but lacked phycoerythrin. R2 contained not only chlorophyll but also a great deal of phycoerythrin. Organic autotrophic particles account for the most proportion of the total particles that were smaller than 50 microm. They represented 70.24% of the total particles. In this study, we found that flow cytometry is particularly suited to the analysis of organic autotrophic particles that are difficult to study with traditional methods.
Guo
Pei-yong
PY
Institute of Environmental Science, Zhejiang University, Hangzhou 310029, China.
Zhu
Yin-mei
YM
D052818:Autotrophic Processes; D015169:Colony Count, Microbial; D005434:Flow Cytometry / Q000379:methods*; D005618:Fresh Water; D008856:Microscopy, Fluorescence; D010316:Particle Size; D014871:Water Microbiology