title: Setaria cervi: immunoprophylactic potential of glutathione-S-transferase against filarial parasite Brugia malayi.
abstract: Glutathione-S-transferase (GST) has been detected in the adult female Setaria cervi, a bovine filarial parasite. The role of S. cervi GST antigen in inducing immunity in the host against Brugia malayi microfilariae and infective larvae was studied by in vitro antibody dependent cell mediated reaction as well as in situ inoculation of filarial parasites within a microchamber in Mastomys. The immune sera from glutathione-S-transferase immunized Mastomys promoted the adherence of peritoneal exudate cells to B. malayi microfilariae and infective larvae in vitro inducing 80.7 and 77.6% cytotoxicity, respectively in 72 h. In the microchambers implanted in the immunized Mastomys host cells could migrate and adhere to the microfilariae and infective larvae and induced 77.8 and 75% cytotoxicity to B. malayi microfilariae and infective larvae in 72 h, respectively. These results suggest that native GST from S. cervi is effective in inducing protection against heterologous B. malayi filarial parasite and thus has potential in immunoprophylaxis.
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Sarika
authors.initials: S
authors.lastname: Gupta
authors.forename: Yashodhar Prabhakar
authors.initials: YP
authors.lastname: Bhandari
authors.forename: Maryada Venkatarami
authors.initials: MV
authors.lastname: Reddy
authors.forename: Bhaskar Chinnaiah
authors.initials: BC
authors.lastname: Harinath
authors.forename: Sushma
authors.initials: S
authors.lastname: Rathaur
mesh_terms: D000330:Aedes / Q000469:parasitology; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology; D017178:Brugia malayi / Q000276:immunology*; D002020:Buffaloes; D004605:Elephantiasis, Filarial / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007814:Larva / Q000276:immunology; D008842:Microfilariae / Q000276:immunology; D009115:Muridae; D018000:Receptors, Peptide / Q000378:metabolism; D017182:Setaria Nematode / Q000201:enzymology / Q000276:immunology*
chemical_list: D018000:Receptors, Peptide; C035560:glutathione receptor; D005982:Glutathione Transferase; D005978:Glutathione