Peptide-based candidate vaccine against respiratory syncytial virus.
We engineered a 21-mer peptide representing amino acids 170-190 of the respiratory syncytial virus (RSV) G protein as a fusion with the Alfalfa mosaic virus (AlMV) coat protein (CP), produced recombinant AlMV particles presenting this peptide (VMR-RSV) on their surfaces and tested the immunogenicity in vitro in human dendritic cells and in vivo in non-human primates. Significant pathogen-specific immune responses were generated in both systems: (i) human dendritic cells armed with VMR-RSV generated vigorous CD4+ and CD8+ T cell responses; (ii) non-human primates that received these particles responded by mounting strong cellular and humoral immune responses. This approach may validate the use of a novel RSV vaccine delivery vehicle in humans.
Yusibov
Vidadi
V
Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark, DE 19711, USA. vyusibov@fraunhofer.org
Mett
Vadim
V
Mett
Valentina
V
Davidson
Carley
C
Musiychuk
Konstantin
K
Gilliam
Suzan
S
Farese
Ann
A
Macvittie
Thomas
T
Mann
Dean
D
D017794:Alfalfa mosaic virus / Q000235:genetics; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology; D005822:Genetic Vectors; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D030821:Plants, Genetically Modified; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control; D012136:Respiratory Syncytial Viruses / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D014026:Nicotiana; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology
D000956:Antigens, Viral; D004279:DNA, Viral; D022223:Vaccines, Subunit; D014765:Viral Vaccines