Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1).
Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.
Yamaguchi
Mineo
M
Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046 Japan.
Sasaki
Takayuki
T
Sivaguru
Mayandi
M
Yamamoto
Yoko
Y
Osawa
Hiroki
H
Ahn
Sung Ju
SJ
Matsumoto
Hideaki
H
D000535:Aluminum / Q000378:metabolism / Q000494:pharmacology*; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004351:Drug Resistance / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D049452:Green Fluorescent Proteins; D019697:Onions / Q000235:genetics / Q000378:metabolism; D027361:Organic Anion Transporters / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D027361:Organic Anion Transporters; D011993:Recombinant Fusion Proteins; C501257:malate transporter protein, Arabidopsis; D049452:Green Fluorescent Proteins; D000535:Aluminum