title: Phylogenetic and biochemical characterisation of a recombinant laccase from Trametes versicolor.
abstract: Laccases are important enzymes for bioremediation and the best-characterised are from the fungus Trametes versicolor. Here, we describe the cloning and characterisation of a new variant of laccase from T. versicolor and its expression in Saccharomyces cerevisiae. We have performed a sequence-based analysis of Trametes laccases that leads to a proposal for a new nomenclature of this fungus laccases according to their phylogenetic relationships since their nomenclature based on IPs is ambiguous. We also describe the kinetic properties of the recombinant enzyme.
authors.affiliation: Departamento de Biotecnología Ambiental, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Mor. 62210, México.
authors.forename: Rosalía
authors.initials: R
authors.lastname: Necochea
authors.forename: Brenda
authors.initials: B
authors.lastname: Valderrama
authors.forename: Silvia
authors.initials: S
authors.lastname: Díaz-Sandoval
authors.forename: Jorge Luis
authors.initials: JL
authors.lastname: Folch-Mallol
authors.forename: Rafael
authors.initials: R
authors.lastname: Vázquez-Duhalt
authors.forename: Gabriel
authors.initials: G
authors.lastname: Iturriaga
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D001673:Biodegradation, Environmental; D042845:Laccase / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D011994:Recombinant Proteins; D042845:Laccase