[Study of rare earth bifunctional chelate BCPDA-Eu3+ labeling protein].
The authors labeled bovine serum albumin (BSA) with a new europium chelator 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) which was synthesized by solid phase time-resolved fluorescence immunoassay (TRFIA) technology. The process of BCPDA-labeling-BSA reaction was studied. Using Coomassie brilliant blue method, concentrations of BSA and BCPDA were determined in BCPDA-BSA labeled sample. The labeling reaction condition was studied. Labeling ratio and protein labeling recovery were calculated. BCPDA-BSA-Eu3+ complex has a very large Stokes shift (270 nm) and can emit very strong fluorescence band at 611.2 nm which is very narrow (10 nm). BCPDA-BSA-Eu3+ complex exhibits very long fluorescence lifetimes by the experiment demonstration. Also, BCPDA and protein-BCPDA-Eu3+ complex is relatively stable.
Pan
Li-hua
LH
National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
An
Jin-long
JL
Xie
Wen-bing
WB
Guan
Ming
M
D000818:Animals; D002417:Cattle; D005063:Europium / Q000737:chemistry*; D015200:Fluoroimmunoassay / Q000379:methods*; D010618:Phenanthrolines / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence
C519844:1,10-phenanthroline-2,9-dicarboxylic acid; D010618:Phenanthrolines; D011506:Proteins; C054206:4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid; D012710:Serum Albumin, Bovine; D005063:Europium; C025205:1,10-phenanthroline