Continuous two-dimensional field-flow fractionation: a novel technique for continuous separation and collection of macromolecules and particles.
Instrumental techniques to analyse macromolecular and particulate materials have undergone rapid development in response to the need for high resolution, precise identification and characterization, and enrichment and collection for further analysis. Continuous two-dimensional field-flow fractionation (2D-FFF), which is described in this article, is a novel technique for separation and collection of macromolecules and particles. 2D-FFF is based on the conventional field-flow fractionation principle but with carrier flow in two-dimensions. This overview discusses the principle of the technique, describes the instrumentation and suggests potential applications and further extensions. An overview of the basic field-flow fractionation principle is presented.
Vastamaki
Pertti
P
Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, FIN-00014, Finland.
Jussila
Matti
M
Riekkola
Marja-Liisa
ML
D005591:Chemical Fractionation; D031521:Fractionation, Field Flow / Q000379:methods*; D046911:Macromolecular Substances; D010316:Particle Size
D046911:Macromolecular Substances