[In vitro inhibition of the cytopathic action of herpes simplex virus, type 1, with a natural cytokine complex].
The antiviral action of a natural cytokine complex (NCC)--the preparation Superlymph and its peptide antimicrobial fraction (AMF)--in the culture of Vero cells infected with type 1 herpes simplex virus (HSV-1), strain VR-3, was studied. The NCC preparation did not alter the morphology of the cells for 6 days and was not toxic for the culture of Vero cells. The NCC and AMF produced a protective antiviral effect, which was manifested by the inhibition of the cytopathic action (CPA) of the virus. In the presence of the preparation, the CPA of HSV-1 was equal to 10(-4.67) ICPD50, while in the control CPA was equal to 10(-5.60). The fraction containing antimicrobial peptides (protegrins) and isolated from NCC, characterized by the method of mass spectrometry, produced the maximum antiviral effect on the cell strain Vero (10(-4.58) ICPD50). Thus Superlymph, an immunomodulator with antiviral activity, could be regarded as an effective preparation for the treatment of HSV infection. The action of such preparation was aimed at the inhibition of the CPA of the virus and the stimulation of the antiviral protective mechanisms of the cell.
Koval'chuk
L V
LV
Lavrov
V F
VF
Gankovskaia
L V
LV
Ebralidze
L K
LK
Barkevich
O A
OA
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D002850:Chromatography, Gel; D016207:Cytokines / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013552:Swine; D014709:Vero Cells
D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents; D016207:Cytokines; D011506:Proteins; C094880:protegrin-1