Diurnal rhythms in plasma cortisol, insulin, glucose, lactate and urea in pigs fed identical meals at 12-hourly intervals.
Diurnal rhythms in plasma cortisol, insulin, glucose, lactate and urea concentrations were investigated in eight catheterized pigs of approximately 35 kg BW. Pigs were fed isoenergetic/isoproteinic diets at a restricted level (2.5 x maintenance requirement for energy) in two daily rations (06:00 and 18:00 hours) in order to obtain equal intervals between feed intake. Preprandial plasma cortisol concentration was 22+/-3 ng/mL in the morning and 14+/-2 ng/mL in the evening (p<0.025), whereas the concentrations of insulin, glucose, lactate, and urea were similar. In the postprandial period in the morning (06:00-09:00 hours) plasma cortisol, insulin and lactate concentrations (expressed as the total area under the curve) were greater (p<0.001) compared to the evening (18:00-21:00 hours) by 100%, 42%, and 24%, respectively, while postprandial plasma glucose and urea concentrations were not affected by time of the meal. When postprandial plasma concentrations were expressed as a response over preprandial concentrations (decremental or incremental area under the curve), the diurnal rhythm was not observed for cortisol and glucose, persisted for insulin and lactate, and appeared for urea with a smaller postprandial urea response (p<0.05) in the morning compared to the evening. We conclude that the diurnal rhythm in plasma cortisol is independent of feeding whereas the diurnal rhythms in plasma insulin, lactate and urea are unveiled by the morning/evening meals in pigs. At equal 12-h intervals between meals, the postprandial responses of lactate and urea show diurnal variations, each in a specific manner, which suggest decreased postprandial efficiency of carbohydrate metabolism and increased postprandial efficiency of protein metabolism in the morning compared to the evening.
Koopmans
Sietse J
SJ
Division of Nutrition and Food, Edelhertweg 15, P.O. Box 65, 8200 AB Lelystad, The Netherlands. sietse-jan.koopmans@wur.nl
van der Meulen
Jan
J
Dekker
Ruud
R
Corbijn
Henk
H
Mroz
Zdzislaw
Z
D000818:Animals; D001341:Autonomic Nervous System / Q000502:physiology; D001786:Blood Glucose / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology*; D004435:Eating / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood*; D007328:Insulin / Q000097:blood*; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D010913:Pituitary-Adrenal System / Q000502:physiology; D019518:Postprandial Period; D013552:Swine / Q000097:blood*; D014508:Urea / Q000097:blood*
D001786:Blood Glucose; D007328:Insulin; D019344:Lactic Acid; D014508:Urea; D006854:Hydrocortisone