A decade of "chromosome painting" in Lolium and Festuca.
GISH has been a particularly useful technique for studying the Lolium-Festuca species complex of forage grasses. The reason for this utility is two-fold: (i) the complex is unique amongst crop plants in which fertile hybrids, and backcross progenies, can be produced which recombine genomes and promiscuously exchange their genes through homoeologous recombination; (ii) dispersed repetitive DNAs differ between species, and this allows tracking of the identity of chromosomes and chromosome segments. This tracking property has enabled several fruitful lines of research to produce a harvest of new information for both fundamental and practical purposes. We review this first decade of GISH (genomic in situ hybridization) in Lolium-Festuca, and discuss and summarize the achievements which have accrued.
Pasakinskiene
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Lithuanian Institute of Agriculture, Dotnuva-Akademija, Kedainiai, Lithuania. izolda@lzi.lt
Jones
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D020223:Chromosome Painting / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics / Q000648:ultrastructure; D019143:Evolution, Molecular; D031751:Festuca / Q000235:genetics*; D018745:Genome, Plant; D017403:In Situ Hybridization; D008129:Lolium / Q000235:genetics*