Frequent overexpression of ETS-related gene-1 (ERG1) in prostate cancer transcriptome.
Transcription factors encoded by the ETS family of genes are central in integrating signals that regulate cell growth and differentiation, stress responses, and tumorigenesis. This study, analysing laser microdissected paired benign and malignant prostate epithelial cells from prostate cancer (CaP) patients (n=114; 228 specimen) by GeneChip and quantitative real-time RT-PCR, identifies ETS-related gene (ERG), a member of the ETS transcription factor family, as the most frequently overexpressed proto-oncogene in the transcriptome of malignant prostate epithelial cells. Combined quantitative expression analysis of ERG with two other genes commonly overexpressed in CaP, AMACR and DD3, revealed overexpression of at least one of these three genes in virtually all CaP specimen (54 of 55). Comprehensive evaluation of quantitative ERG1 expression with clinicopathological features also suggested that ERG1 expression level in prostate tumor cells relative to benign epithelial cells is indicator of disease-free survival after radical prostatectomy.
Petrovics
Gyorgy
G
Center for Prostate Disease Research (CPDR), Department of Surgery and US Military Cancer Institute, Uniformed Services University, Rockville, MD 20852, USA. gpetrovics@cpdr.org
Liu
Aijun
A
Shaheduzzaman
Syed
S
Furusato
Bungo
B
Furasato
Bungo
B
Sun
Chen
C
Chen
Yongmei
Y
Nau
Martin
M
Ravindranath
Lakshmi
L
Chen
Yidong
Y
Dobi
Albert
A
Srikantan
Vasantha
V
Sesterhenn
Isabell A
IA
McLeod
David G
DG
Vahey
Maryanne
M
Moul
Judd W
JW
Srivastava
Shiv
S
D000951:Antigens, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D019998:Racemases and Epimerases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic; D000071230:Transcriptional Regulator ERG
D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; C072312:ERG protein, human; D007527:Isoenzymes; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG; C479341:prostate cancer antigen 3, human; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase