title: An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast.
abstract: Three groups of fungicides (phenylpyrroles, dicarboximides, aromatic hydrocarbons) are effective against filamentous fungi. The target of these fungicides is the osmotic stress signal transduction pathway, which is dependent on the Os-1 family of two-component histidine kinases. These fungicides usually have no fungicidal effect on the yeast Saccharomyces cerevisiae. In this report, we found that expression of Hik1, an Os-1 orthologue from rice blast fungus, can confer fungicide-sensitivity to yeast. This requires both the histidine kinase and the response regulator domains of Hik1. Analysis of yeast mutants indicated that this sensitivity is Hog1- and Ssk1-dependent. In addition, our studies revealed an interaction between Hik1 and Ypd1. These observations suggest that Hik1 is a direct target of the fungicides or is a mediator of fungicide action and that the fungicidal effect is transmitted to the Hog1 pathway via Ypd1.
authors.affiliation: RIKEN (Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan. tmotoyam@riken.jp
authors.forename: Takayuki
authors.initials: T
authors.lastname: Motoyama
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ohira
authors.forename: Kaori
authors.initials: K
authors.lastname: Kadokura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ichiishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimura
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kudo
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D000071677:Histidine Kinase; D047908:Intracellular Signaling Peptides and Proteins; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000235:genetics; D020082:Magnaporthe / Q000201:enzymology* / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D005659:Fungicides, Industrial; D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C102035:YPD1 protein, S cerevisiae; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases; D000071677:Histidine Kinase