title: Transduced Tat-SOD fusion protein protects against ischemic brain injury.
abstract: Reactive oxygen species (ROS) are implicated in reperfusion injury after transient focal cerebral ischemia. The antioxidant enzyme, Cu,Zn-superoxide dismutase (SOD), is one of the major means by which cells counteract the deleterious effects of ROS after ischemia. Recently, we reported that when Tat-SOD fusion protein is transduced into pancreatic beta cells it protects the beta cells from destruction by relieving oxidative stress in ROS-implicated diabetes (Eum et al., 2004). In the present study, we investigated the protective effects of Tat-SOD fusion protein against neuronal cell death and ischemic insults. When Tat-SOD was added to the culture medium of neuronal cells, it rapidly entered the cells and protected them against paraquat-induced cell death. Immunohistochemical analysis revealed that Tat-SOD injected intraperitoneally (i.p.) into mice has access to various tissues including brain neurons. When i.p. injected into gerbils, Tat-SOD prevented neuronal cell death in the hippocampus in response to transient fore-brain ischemia. These results suggest that Tat-SOD provides a strategy for therapeutic delivery in various hu-man diseases, including stroke, related to this anti-oxidant enzyme or to ROS.
authors.affiliation: Department of Genetic Engineering and Research, Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Choi
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Choi
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: An
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Kyuhyung
authors.initials: K
authors.lastname: Han
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kang
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Tae Yoon
authors.initials: TY
authors.lastname: Kim
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D015696:Gene Products, tat / Q000627:therapeutic use; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D014161:Transduction, Genetic
chemical_list: D000975:Antioxidants; D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D013482:Superoxide Dismutase