title: Expression profiling of the estrogen responsive genes in response to phytoestrogens using a customized DNA microarray.
abstract: Here, we examined phytoestrogens, isoflavones (genistein, daidzein, glycitein, biochanin A and ipriflavone), flavones (chrysin, luteolin and apigenin), flavonols (kaempferol and quercetin), and a coumestan, a flavanone and a chalcone (coumestrol, naringenin and phloretin, respectively) by means of a DNA microarray assay. A total of 172 estrogen responsive genes were monitored with a customized DNA microarray and their expression profiles for the above phytoestrogens were compared with that for 17beta-estradiol (E2) using correlation coefficients, or R values, after a correlation analysis by linear regression. While R values indicate the similarity of the response by the genes, we also examined the genes by cluster analysis and by their specificity to phytoestrogens (specific to genistein, daidzein or glycitein) or gene functions. Several genes were selected from p53-related genes (CDKN1A, TP53I11 and CDC14), Akt2-related genes (PRKCD, BRCA1, TRIB3 and APPL), mitogen-activated protein kinase-related genes (RSK and SH3BP5), Ras superfamily genes (RAP1GA1, RHOC and ARHGDIA) and AP-1 family and related genes (RIP140, FOS, ATF3, JUN and FRA2). We further examined the extracts from two local crops of soy beans (Kuro-daizu or Mochi-daizu) by comparing the gene expression profiles with those of E2 or phytoestrogens as a first step in utilizing the expression profiles for various applications.
authors.affiliation: InfoGenes Co., Ltd., Tsukuba, Ibaraki 305-0047, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Ise
authors.forename: Dalho
authors.initials: D
authors.lastname: Han
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Shunichi
authors.initials: S
authors.lastname: Terasaka
authors.forename: Akio
authors.initials: A
authors.lastname: Inoue
authors.forename: Masao
authors.initials: M
authors.lastname: Tanji
authors.forename: Ryoiti
authors.initials: R
authors.lastname: Kiyama
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation; D004958:Estradiol / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D048789:Phytoestrogens / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D013025:Glycine max / Q000737:chemistry; D015854:Up-Regulation
chemical_list: D048789:Phytoestrogens; D010936:Plant Extracts; D004958:Estradiol