title: Distribution of C-->T and T-->C polymorphisms of the urokinase-type plasminogen activator gene in children with type 1 diabetes mellitus and insulin resistance.
abstract: We analysed the distribution of genotypes and frequency of alleles of two polymorphisms in the urokinase-type plasminogen activator (uPA) gene: a C-->T substitution in exon 6 and a T-->C substitution in intron 7 in 89 children with type 1 diabetes mellitus and insulin resistance compared with 120 non-diabetic control subjects. All genotypes were determined by the allele-specific polymerase chain reaction. We found that the frequency of the T/T homozygote (15%) in the patient group was significantly (P<0.05) higher than in the controls (7%). There were no differences in the distribution of the T-->C polymorphism between patients and controls, which suggests that this genetic change is probably phenotypically silent. In conclusion, our results indicate that the higher percentage of T/T homozygotes in patients might be associated with T1DM coexisting with insulin resistance.
authors.affiliation: Department of Molecular Genetics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Majsterek
authors.forename: K
authors.initials: K
authors.lastname: Przybylowska
authors.forename: W
authors.initials: W
authors.lastname: Mlynarski
authors.forename: J
authors.initials: J
authors.lastname: Drzewoski
authors.forename: J
authors.initials: J
authors.lastname: Blasiak
mesh_terms: D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
chemical_list: D014568:Urokinase-Type Plasminogen Activator