title: Genomic organization, promoter characterization and roles of Sp1 and AP-2 in the basal transcription of mouse PDIP1 gene.
abstract: The mouse polymerase delta-interacting protein 1 gene, PDIP1, is mapped to chromosome 7F3 region, spans approximately 16.7kb, and is organized into six exons. The transcription start site (TSS) was determined to be G, corresponding to position of 162-bp upstream of the translation start codon. The promoter region was found to lack TATA box or CCAAT box, instead, a CpG island was detected surrounding TSS. The region from -162 to +114 is required for basal transcriptional regulation of mouse PDIP1 gene, contains two AP-2 and two Sp1 binding sites. The Sp1 site upstream of TSS activates, while the other Sp1 site and two AP-2 sites suppress the transcription activity of mouse PDIP1 gene.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Zhou
authors.forename: Changzheng
authors.initials: C
authors.lastname: Fan
authors.forename: Yingli
authors.initials: Y
authors.lastname: Zhong
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Liu
authors.forename: Mingjun
authors.initials: M
authors.lastname: Liu
authors.forename: Aidong
authors.initials: A
authors.lastname: Zhou
authors.forename: Kaiqun
authors.initials: K
authors.lastname: Ren
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation; D005091:Exons / Q000235:genetics; D040461:Gene Components; D016678:Genome; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000502:physiology*; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D043743:Ubiquitin-Protein Ligase Complexes; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D050656:Transcription Factor AP-2; D014157:Transcription Factors; C499862:PDIP1 protein, mouse; D043743:Ubiquitin-Protein Ligase Complexes