title: Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway.
abstract: The substrates for Bacillus subtilis PLP synthase (YaaD and YaaE) are identified, and the first reconstitution of PLP biosynthesis using this pathway is described. Three partial reactions catalyzed by YaaD are also identified.
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Burns
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Kinsland
authors.forename: Fred W
authors.initials: FW
authors.lastname: McLafferty
authors.forename: Tadhg P
authors.initials: TP
authors.lastname: Begley
mesh_terms: D000878:Anthranilate Synthase / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D005583:Fourier Analysis; D005972:Glutaminase / Q000378:metabolism; D019884:Nitrogenous Group Transferases / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000096:biosynthesis*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001426:Bacterial Proteins; D011732:Pyridoxal Phosphate; D019884:Nitrogenous Group Transferases; D005972:Glutaminase; D000878:Anthranilate Synthase; C020655:anthranilate synthase, glutamine amidotransferase subunit