title: Chemotaxonomic identification of single bacteria by micro-Raman spectroscopy: application to clean-room-relevant biological contaminations.
abstract: Microorganisms, such as bacteria, which might be present as contamination inside an industrial food or pharmaceutical clean room process need to be identified on short time scales in order to minimize possible health hazards as well as production downtimes causing financial deficits. Here we describe the first results of single-particle micro-Raman measurements in combination with a classification method, the so-called support vector machine technique, allowing for a fast, reliable, and nondestructive online identification method for single bacteria.
authors.affiliation: Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Rösch
authors.forename: Michaela
authors.initials: M
authors.lastname: Harz
authors.forename: Michael
authors.initials: M
authors.lastname: Schmitt
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Peschke
authors.forename: Olaf
authors.initials: O
authors.lastname: Ronneberger
authors.forename: Hans
authors.initials: H
authors.lastname: Burkhardt
authors.forename: Hans-Walter
authors.initials: HW
authors.lastname: Motzkus
authors.forename: Markus
authors.initials: M
authors.lastname: Lankers
authors.forename: Stefan
authors.initials: S
authors.lastname: Hofer
authors.forename: Hans
authors.initials: H
authors.lastname: Thiele
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000145:classification; D001419:Bacteria / Q000737:chemistry* / Q000145:classification* / Q000254:growth & development; D015373:Bacterial Typing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D003627:Data Interpretation, Statistical; D004780:Environment, Controlled; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification; D008837:Micrococcus / Q000737:chemistry / Q000145:classification; D038761:Photobleaching; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013171:Spores, Bacterial / Q000737:chemistry; D013210:Staphylococcus / Q000737:chemistry / Q000145:classification