[Observation and research on the electroporation of S-180 cell induced by electric pulse].
This is our first time report of S-180 cell handled with various voltages, capacity and pulse number. Many pores on the S-180 cell membrane can be observed under scanning electron microscope. By the stains of trypan blue, we have known the influence of electric parameters on the ratio of poration. The results show that the ratio of electroporation has positive correlation with the voltages and the pulse number while negative correlation with the capacity.
Li
Hong
H
College of Life Science, Sichuan University, Chengdu 610064, China.
Geng
Qin
Q
Yue
Bisong
B
Zou
Fangdong
F
Wang
Baoyi
B
Zhang
Hong
H
Zeng
Hao
H
D000818:Animals; D002463:Cell Membrane Permeability; D004574:Electromagnetic Fields; D018274:Electroporation / Q000379:methods; D051379:Mice; D012510:Sarcoma 180 / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured