title: Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis.
abstract: Prenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis.
authors.affiliation: Danek Gertner Institute of Human Genetics, Sheba Medical Center, Tel-Hashomer, Israel 52621. Esther.Guetta@sheba.health.gov.il
authors.forename: Esther
authors.initials: E
authors.lastname: Guetta
authors.forename: Liat
authors.initials: L
authors.lastname: Gutstein-Abo
authors.forename: Gad
authors.initials: G
authors.lastname: Barkai
mesh_terms: D002499:Centrifugation, Density Gradient; D005260:Female; D006680:HLA Antigens / Q000378:metabolism; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D018189:Immunomagnetic Separation; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D012732:Sex Determination Analysis / Q000379:methods; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I