title: Magnetic field inhibits isolated lymphocytes' proliferative response to mitogen stimulation.
abstract: We aimed to find out how the exposure of isolated lymphocytes to a pulsed magnetic field (MF) affected their in vitro proliferative response to mitogenic stimulation. Cells were exposed to MF of various intensities (0.3, 0.6, and 1.2 T) at a constant frequency of 30 Hz, for a period of 60, 180, and 330 s. Then, the proliferative response of splenocytes was induced by optimal concentrations of concanavalin A (Con A; mitogenic toward T cells), bacterial lipopolysaccharide (LPS; mitogenic toward B cells), or pokeweed mitogen (PWM; mitogenic toward both populations). We found that the exposure of lymphocytes to the MF profoundly inhibited their proliferative response to mitogens. The suppressive action of the MF on B and T cell proliferation was intensified when a cooperative response of those two lymphocyte populations was simultaneously induced by PWM. The inhibitory effect of MF depended on the exposure time and MF intensity. Prolonged exposure and/or a stronger intensity of the MF weakened its inhibitory influence on the response of lymphocyte to mitogenic stimulation. The data show that an exposure to MF may influence the activity of lymphocytes in their response to mitogenic stimuli.
authors.affiliation: Laboratory of Intracellular Signalling, Department of Biochemistry, Institute of Pharmacology, Polish Academy of Sciences, 31-343 Kraków, Poland. roman@if-pan.krakow.pl
authors.forename: Adam
authors.initials: A
authors.lastname: Roman
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zyss
authors.forename: Irena
authors.initials: I
authors.lastname: Nalepa
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D006133:Growth Substances / Q000008:administration & dosage*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology / Q000528:radiation effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects*; D008297:Male; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006133:Growth Substances