title: Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis.
abstract: Intraspecies variation of Chromobacterium violaceum was examined by comparative sequence - and by restriction fragment length polymorphism analysis of the recombinase A gene (recA-PCR-RFLP). Primers deduced from the known recA gene sequence of the type strain C. violaceum ATCC 12472(T) allowed the specific amplification of a 1040bp recA fragment from each of the 13 C. violaceum strains investigated, whereas other closely related organisms tested negative. HindII-PstI-recA RFLP analysis generated from 13 representative C. violaceum strains enabled us to identify at least three different genospecies. In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level.
authors.affiliation: Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany. holger1scholz@bundeswehr.org
authors.forename: Holger Christian
authors.initials: HC
authors.lastname: Scholz
authors.forename: Angela
authors.initials: A
authors.lastname: Witte
authors.forename: Herbert
authors.initials: H
authors.lastname: Tomaso
authors.forename: Sascha
authors.initials: S
authors.lastname: Al Dahouk
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
mesh_terms: D015373:Bacterial Typing Techniques; D002861:Chromobacterium / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011938:Rec A Recombinases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D011938:Rec A Recombinases