Expression of Ndrg2 in the rat frontal cortex after antidepressant and electroconvulsive treatment.
Although the therapeutic action of antidepressants most likely involves the regulation of serotonergic and noradrenergic signal transduction, no consensus has been reached concerning their precise molecular or cellular mechanisms of action. In the present study, we demonstrated that chronic treatment with a tricyclic antidepressant (imipramine) and a selective serotonin reuptake inhibitor (sertraline) reduced the expression of Ndrg2 mRNA and protein in the rat frontal cortex. Ndrg2 is a member of the N-Myc downstream-regulated genes. Interestingly, repeated ECT also significantly decreased Ndrg2 expression in this region of the brain. These data suggest that Ndrg2 may be a common functional molecule that is decreased after antidepressant treatment and ECT. Although, the functional role of Ndrg2 in the central nervous system remains unclear, our findings suggest that Ndrg2 may be associated with treatment-induced adaptive neural plasticity in the brain, a chronic target of antidepressant action. In conclusion, we have identified Ndrg2 as a candidate target molecule of antidepressants and ECT.
Takahashi
Kou
K
Department of Pharmacology, Showa University School of Pharmaceutical Sciences, Tokyo, Japan.
Yamada
Misa
M
Ohata
Hisayuki
H
Momose
Kazutaka
K
Higuchi
Teruhiko
T
Honda
Kazuo
K
Yamada
Mitsuhiko
M
D000704:Analysis of Variance; D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage*; D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D004334:Drug Administration Schedule; D004597:Electroshock / Q000379:methods*; D005625:Frontal Lobe / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D015870:Gene Expression / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014162:Transfection / Q000379:methods
D000928:Antidepressive Agents; C507213:Ndrg2 protein, rat; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger