title: The beta subunit determines the ligand binding properties of synaptic glycine receptors.
abstract: Inhibitory glycine receptors (GlyRs) regulate motor coordination and sensory signal processing in spinal cord and other brain regions. GlyRs are pentameric proteins composed of membrane-spanning alpha and beta subunits. Here, site-directed mutagenesis combined with homology modeling based on the crystal structure of the acetylcholine binding protein identified key ligand binding residues of recombinant homooligomeric alpha1 and heterooligomeric alpha1beta GlyRs. This disclosed two highly conserved, oppositely charged residues located on adjacent subunit interfaces as being crucial for agonist binding. In addition, the beta subunit was found to determine the ligand binding properties of heterooligomeric GlyRs. Expression of an alpha1beta tandem construct and affinity purification of metabolically labeled GlyRs confirmed a subunit stoichiometry of 2alpha3beta. Because the beta subunit anchors GlyRs at synaptic sites, our results have important implications for the biosynthesis, clustering, and pharmacology of synaptic GlyRs.
authors.affiliation: Department of Neurochemistry, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, D-60528 Frankfurt am Main, Germany.
authors.forename: Joanna
authors.initials: J
authors.lastname: Grudzinska
authors.forename: Rudolf
authors.initials: R
authors.lastname: Schemm
authors.forename: Svenja
authors.initials: S
authors.lastname: Haeger
authors.forename: Annette
authors.initials: A
authors.lastname: Nicke
authors.forename: Guenther
authors.initials: G
authors.lastname: Schmalzing
authors.forename: Heinrich
authors.initials: H
authors.lastname: Betz
authors.forename: Bodo
authors.initials: B
authors.lastname: Laube
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D018009:Receptors, Glycine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013331:Strychnine / Q000378:metabolism / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: C114561:GLRB protein, human; D008024:Ligands; D018009:Receptors, Glycine; D013331:Strychnine; D005998:Glycine