Molecular profiling of Bacteroides spp. in human feces by PCR-temperature gradient gel electrophoresis.
A group-specific PCR-based temperature gradient gel electrophoresis (TGGE) method was developed to study the population composition of genus Bacteroides in human gut. Highly reproducible and well-separated bands in TGGE fingerprints of ten unrelated human fecal samples showed complex and host-specific Bacteroides species composition. Dynamic monitoring over 22 months of samples from one healthy 10-year-old boy indicated a relatively stable population profile of Bacteroides. The species identity of each band in TGGE gel of this boy was also resolved via comigration analysis of sequenced inserts in a Bacteroides group-specific clone library. This work provides a rapid and effective technique for analyzing the species composition of Bacteroides in human gut.
Pang
Xiaoyan
X
Laboratory of Molecular Microbial Ecology and Ecogenomics, Department of Bioscience and Biotechnology, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Ding
Dezhong
D
Wei
Guifang
G
Zhang
Meiling
M
Wang
Linghua
L
Zhao
Liping
L
D015373:Bacterial Typing Techniques; D001439:Bacteroides / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D002648:Child; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D004269:DNA, Bacterial