Expression of cGMP-dependent protein kinases (I and II) and neuronal nitric oxide synthase in the developing rat cerebellum.
The expression of neuronal nitric oxide synthase (nNOS) and the cGMP-dependent protein kinases cGKI and cGKII in rat cerebellum was evaluated at different developmental stages by quantitative RT-PCR and Western blotting. mRNAs coding for these proteins were detected in the cerebella of rats aged 7, 14 and 21 days. Expression levels, nevertheless, varied significantly at each of these developmental stages. While nNOS and cGKI mRNA levels steadily increased during development, cGKII mRNA showed a different behaviour pattern, with similar levels observed on postnatal days 7 and 14 and increased levels noted on postnatal day 21. Moreover, protein expression profiles for nNOS and cGKI showed similar patterns to the mRNAs encoding these proteins. Our results reveal the developmental regulation of the expression of these proteins in the cerebellum, giving rise to higher levels as the cerebellum matures.
Jurado
Sandra
S
Department de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
Sánchez-Prieto
José
J
Torres
Magdalena
M
D000375:Aging / Q000378:metabolism; D000818:Animals; D002531:Cerebellum / Q000201:enzymology* / Q000254:growth & development*; D006152:Cyclic GMP / Q000378:metabolism; D062965:Cyclic GMP-Dependent Protein Kinase Type I; D062967:Cyclic GMP-Dependent Protein Kinase Type II; D017869:Cyclic GMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D062965:Cyclic GMP-Dependent Protein Kinase Type I; D062967:Cyclic GMP-Dependent Protein Kinase Type II; D017869:Cyclic GMP-Dependent Protein Kinases; C569732:Prkg2 protein, rat; D006152:Cyclic GMP