Micromonospora mirobrigensis sp. nov.
An actinomycete strain was recovered from a pond where radon is known to be dissolved. A polyphasic study was undertaken to identify the new isolate. The 16S rRNA gene sequence of strain WA201(T) showed closest similarity to the type strains of Micromonospora carbonacea (98.5 %) and Micromonospora matsumotoense (98.1 %). The chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Micromonospora. DNA-DNA relatedness values supported the classification of this isolate as a novel species. A number of physiological and biochemical tests were able to distinguish strain WA201(T) from its closest phylogenetic neighbours. Therefore, it is proposed that isolate WA201(T) (=DSM 44830(T)=LMG 22229(T)) be considered the type strain representing a novel species, Micromonospora mirobrigensis sp. nov.
Trujillo
Martha E
ME
Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Fernández-Molinero
Carmen
C
Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Velázquez
Encarna
E
Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Kroppenstedt
Reiner M
RM
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
Mateos
Pedro F
PF
Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Martínez-Molina
Eustoquio
E
Departamento de Microbiología y Genética, Edificio Departamental, Lab 209, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D008847:Micromonospora / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011886:Radon; D017422:Sequence Analysis, DNA; D014875:Water Pollutants, Radioactive
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014875:Water Pollutants, Radioactive; D011886:Radon