title: [Examination of the RHD gene by non-invasive method in plasma of alloimmunized mother allowed to abandon invasive procedures and to deliver a healthy child].
abstract: RHD gen of the fetus was examined by a non-invasive method in pregnant woman with a high titre of anti-D antibody, developed during previous pregnancy, which resulted in haemolytic disease of the newborn. For RHD gene evaluation, the DNA from maternal plasma was examined, by the use of real-time PCR. RHD gene was not detected. Additional detection of gen GSTM1 in maternal plasma,which was present in the father but absent in the mother, assured us that we examined the fetal, not maternal RHD gene. Thus, the cordocentesis was not performed. The mother delivered healthy RhD negative daughter.
authors.affiliation: Instytutu Hematologii i Transfuzjologii, Warszawie.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Orzińska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Guz
authors.forename: Ewa
authors.initials: E
authors.lastname: Brojer
authors.forename: Barbara
authors.initials: B
authors.lastname: Zupańska
mesh_terms: D000328:Adult; D001788:Blood Grouping and Crossmatching / Q000379:methods; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000097:blood / Q000276:immunology*; D011256:Pregnancy Outcome; D011296:Prenatal Diagnosis / Q000379:methods; D012203:Rh Isoimmunization / Q000097:blood / Q000235:genetics*; D018029:Rho(D) Immune Globulin / Q000097:blood / Q000235:genetics*; D012307:Risk Factors; D012680:Sensitivity and Specificity
chemical_list: D018029:Rho(D) Immune Globulin