title: Cross-reactivity to highly pathogenic avian influenza H5N1 viruses after vaccination with nonadjuvanted and MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine: a potential priming strategy.
abstract: Antigenically well-matched vaccines against highly pathogenic avian influenza H5N1 viruses are urgently required. Human serum samples after immunization with MF59 or nonadjuvanted A/duck/Singapore/97 (H5N3) vaccine were tested for antibody to 1997-2004 human H5N1 viruses. Antibody responses to 3 doses of nonadjuvanted vaccine were poor and were higher after MF59-adjuvanted vaccine, with seroconversion rates to A/HongKong/156/97, A/HongKong/213/03, A/Thailand/16/04, and A/Vietnam/1203/04 of 100% (P < .0001), 100% (P < .0001), 71% (P = .0004), and 43% (P = .0128) in 14 subjects, respectively, compared with 27%, 27%, 0%, and 0% in 11 who received nonadjuvanted vaccine. These findings have implications for the rational design of pandemic vaccines against influenza H5.
authors.affiliation: Influenza Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. iain.stephenson@uhl-tr-nhs.uk
authors.forename: Iain
authors.initials: I
authors.lastname: Stephenson
authors.forename: Roberto
authors.initials: R
authors.lastname: Bugarini
authors.forename: Karl G
authors.initials: KG
authors.lastname: Nicholson
authors.forename: Audino
authors.initials: A
authors.lastname: Podda
authors.forename: John M
authors.initials: JM
authors.lastname: Wood
authors.forename: Maria C
authors.initials: MC
authors.lastname: Zambon
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Katz
mesh_terms: D000276:Adjuvants, Immunologic; D000293:Adolescent; D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology*; D004372:Ducks / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006723:Hong Kong / Q000453:epidemiology; D006801:Humans; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000600:supply & distribution; D005585:Influenza in Birds / Q000453:epidemiology / Q000276:immunology* / Q000517:prevention & control / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D009975:Orthomyxoviridae / Q000145:classification* / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D012846:Singapore / Q000453:epidemiology; D013785:Thailand / Q000453:epidemiology; D014611:Vaccination; D014744:Vietnam / Q000453:epidemiology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007252:Influenza Vaccines