Novel antibody-lectin enzyme-linked immunosorbent assay that distinguishes prion proteins in sporadic and variant cases of Creutzfeldt-Jakob disease.
We used different anti-prion protein (anti-PrP) monoclonal antibodies to capture either full-length or truncated PrP species and then used biotinylated lectin to compare the nature of the glycans on bound PrP species present in control, sporadic Creutzfeldt-Jakob disease (sCJD), or variant CJD (vCJD) brains. When full-length PrP species in these three groups were compared, no significant difference in the binding of concanavalin A or Aleuria aurantia lectin was detected. However, the binding of Ricinus communis agglutinin I (RCA) to sCJD and vCJD samples was significantly increased. In contrast, when only truncated PrP species were compared, only vCJD samples had more RCA binding activity. Therefore, while most of the RCA binding activity in sCJD is restricted to the full-length PrP species, the RCA binding activity in vCJD is associated with truncated and full-length PrP species. Furthermore, the RCA binding activity in sCJD and vCJD samples is mostly associated with proteinase K-resistant PrP species, a known signature of infectious prion. Therefore, PrP species in sCJD and vCJD have dissimilar lectin immunoreactivity, which reflects differences in their N-linked glycans. These differences may account for the distinct phenotypes of sCJD and vCJD.
Pan
Tao
T
Institute of Pathology, Biomedical Research Building, Case Western University School of Medicine, Room 933, 10900 Euclid Ave., Cleveland, OH 44120, USA.
Li
Ruliang
R
Wong
Boon-Seng
BS
Kang
Shin-Chung
SC
Ironside
James
J
Sy
Man-Sun
MS
D019921:Biotinylation; D007562:Creutzfeldt-Jakob Syndrome / Q000175:diagnosis*; D003937:Diagnosis, Differential; D019286:Endopeptidase K / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D037121:Plant Lectins / Q000276:immunology; D011134:Polysaccharides / Q000032:analysis*; D011328:Prions / Q000032:analysis*
D037121:Plant Lectins; D011134:Polysaccharides; D011328:Prions; C017707:Ricinus communis agglutinin-1; D019286:Endopeptidase K