Application of a newly developed ARB software-integrated tool for in silico terminal restriction fragment length polymorphism analysis reveals the dominance of a novel pmoA cluster in a forest soil.
TRF-CUT, an ARB-implemented tool, was developed to predict in silico the terminal restriction fragments of aligned small-subunit rRNA gene or functional gene sequences. Application of this new tool to perform directed terminal restriction fragment length polymorphism analysis of pmoA products obtained from a forest soil revealed that novel cluster I methanotrophic bacteria were dominant.
Ricke
Peter
P
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany.
Kolb
Steffen
S
Braker
Gesche
G
D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D017753:Ecosystem; D005798:Genes, Bacterial; D005858:Germany; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012984:Software; D012988:Soil Microbiology