[Cultivation of the vaccine strain of the virus of porcine classic plague].
Several cell cultures were compared and examined for their capability to support reproduction of Classical Swine Fever Virus (CSFV). Experimental conditions of virus cultivation were optimized. The subculture of primary lamb testicular (LT) cells was selected for large cell cultivation of CSFV. In contrast to many cell lines, these cells were free of Bovine Viral Diarrhea Virus (BVDV) contamination partly due to liquid nitrogen storage and gamma-ray sterilization of serum used in the growth media. The vaccine strain CS of CSFV was obtained in the titers of 6.7-7.7 TCID50/ml under the optimal conditions of virus reproduction in the LT cells.
Koritskaia
M A
MA
Demkina
M M
MM
Sergeev
V A
VA
D000818:Animals; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000254:growth & development*; D012756:Sheep; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D014776:Virus Cultivation
D014613:Vaccines, Attenuated; D014765:Viral Vaccines