title: ATP depletion and cell death in the neonatal lamb ductus arteriosus.
abstract: Postnatal constriction of the full-term ductus arteriosus produces cell death and remodeling of the ductus wall. Using a bioluminescence imaging technique, we found that after birth, the lamb ductus develops ATP, glucose, and glycogen depletion in addition to hypoxia. In vitro studies showed that cell death correlates best with ATP depletion and is most marked when both glucose and oxygen are severely depleted; in addition, the degree of ATP depletion found in vivo is sufficient to account for the extensive degree of cell death that occurs after birth. Under hypoxic conditions, the immature ductus is more capable of preserving its ATP supply than the mature ductus as a result of increased glucose availability, glycogen stores, and glucose utilization. However, the immature ductus is just as susceptible as the mature ductus to ATP depletion when glucose supplies are restricted. The extensive degree of cell death that occurs in the newborn ductus after birth is associated primarily with ATP depletion. The increased glycolytic capacity of the immature ductus may enable it to tolerate episodes of hypoxia and nutrient shortage, making it more resistant to developing postnatal cell death and permanent closure.
authors.affiliation: Wallenberg Laboratory for Cardiovascular Research, Göteborg University, SE-413 45 Göteborg, Sweden.
authors.forename: Max
authors.initials: M
authors.lastname: Levin
authors.forename: Seth
authors.initials: S
authors.lastname: Goldbarg
authors.forename: Anders
authors.initials: A
authors.lastname: Lindqvist
authors.forename: Karl
authors.initials: K
authors.lastname: Swärd
authors.forename: Christine
authors.initials: C
authors.lastname: Roman
authors.forename: Bao Mei
authors.initials: BM
authors.lastname: Liu
authors.forename: Lillemor Mattsson
authors.initials: LM
authors.lastname: Hultén
authors.forename: Jan
authors.initials: J
authors.lastname: Borén
authors.forename: Ronald I
authors.initials: RI
authors.lastname: Clyman
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D004373:Ductus Arteriosus / Q000166:cytology* / Q000378:metabolism*; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D007003:Hypoglycemia / Q000378:metabolism / Q000473:pathology; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology; D066298:In Vitro Techniques; D012756:Sheep
chemical_list: D000255:Adenosine Triphosphate; D005947:Glucose