[An experimental study of the effect of IL-2 on the growth of irradiated intestinal epithelial cells].
OBJECTIVE
To observe the effect of ionizing radiation on intestinal epithelial cells 6 (IEC-6) and the effect of IL-2 on the proliferation and recovery of radiation-injured IEC-6 cells.


METHODS
IEC-6 cells exposed to 4, 8 and 12 Gy gamma ray were analyzed with MTT colorimetry, light microscopy, electron microscopy, DNA gel electrophoresis and flow cytometry at 3, 6, 9, 12 hours and 1, 2, 3 days after radiation. Proliferation of 8 Gy gamma ray-irradiated IEC-6 cells which was treated with IL-2 was detected by MTT colorimetry at 3, 6, 9, 12 and 24 hours after irradiation.


RESULTS
Proliferation of IEC-6 decreased with the increased dose of gamma ray radiation. Apoptotic IEC-6 cells increased apparently at 24 hours after radiation with 8.0 Gy gamma ray, IL-2 could enhance the proliferation of irradiated IEC-6 cells (especially at concentration of 1 x 10(5) U/L) in a dose-dependent manner.


CONCLUSIONS
4-12 Gy of gamma ray radiation can reduce the proliferation and cause apoptosis of IEC-6 cells. IL-2 can accelerate proliferation of irradiated IEC-6 cells and protect IECs from ionization radiation injury.
Fu
Kai-fei
KF
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Peng
Rui-yun
RY
Gao
Ya-bing
YB
Wang
De-wen
DW
Luo
Qing-liang
QL
Yang
Yi
Y
Dong
Bo
B
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004847:Epithelial Cells / Q000187:drug effects* / Q000528:radiation effects* / Q000648:ultrastructure; D005720:Gamma Rays / Q000009:adverse effects; D007376:Interleukin-2 / Q000494:pharmacology*; D007422:Intestines / Q000166:cytology*; D009336:Necrosis; D013997:Time Factors
D007376:Interleukin-2