title: A neuronal cell line that does not express either prion or doppel proteins.
abstract: Prions have been extensively studied since they represent a new class of infectious agents, the pathogenic prion protein (PrPSc). However, a central question on the physiological function of the normal prion protein (PrPC) remains unresolved. A cell model which was previously established from Rikn mice (PrP-/-) remains problematic because of its ectopic expression of the doppel (Dpl) which may have a neurotoxic effect. Here we established neuronal cell lines from Zürich I (PrP-/-) which do not express Dpl protein and ICR mice (PrP+/+) by transfecting with plasmid encoding for the large T antigen of SV40. The transformed cells have shown neuronal characteristics and, thus, these cell lines may provide a useful model to explore the function of neuronal PrPC.
authors.affiliation: Ilsong Institute of Life Science, College of Medicine, Hallym University, Ilsong Building, Anyang, Kyonggi-do 431-060, South Korea.
authors.forename: Boe-Hyun
authors.initials: BH
authors.lastname: Kim
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Choi
authors.forename: Richard I
authors.initials: RI
authors.lastname: Carp
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count / Q000379:methods; D002453:Cell Cycle / Q000502:physiology; D002461:Cell Line, Transformed / Q000378:metabolism; D048429:Cell Size; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique / Q000379:methods; D058851:GPI-Linked Proteins; D006624:Hippocampus / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D018621:PrPC Proteins / Q000235:genetics / Q000378:metabolism*; D011328:Prions / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
chemical_list: D058851:GPI-Linked Proteins; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D018621:PrPC Proteins; D011328:Prions; C122036:Prnd protein, mouse; D012333:RNA, Messenger; D010751:Phosphopyruvate Hydratase