title: Identification of volatile basic components in tobacco by headspace liquid-phase microextraction coupled to matrix-assisted laser desorption/ionization with Fourier transform mass spectrometry.
abstract: A method incorporating headspace liquid-phase microextraction (HS-LPME) coupled to matrix-assisted laser desorption/ionization (MALDI) with Fourier transform mass spectrometry (FTMS) was established to analyze volatile basic components in tobacco. The sample preparation volume for MALDI-MS was compatible with the volume of the solvent microdrop in the HS-LPME procedure. The pH and the polarity of the solvent for HS-LPME were adjusted by choice of the MALDI matrix and matrix additive. Based on the elemental composition and tandem mass spectrometry information, 25 volatile nitrogenous compounds in tobacco were detected and identified. The approach is fast and sensitive, and has the potential for automation for high-throughput analysis. This approach offers an alternative method for analysis of trace volatile organic compounds in complex samples.
authors.affiliation: Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Science, Shanghai, 200032, China.
authors.forename: Shihao
authors.initials: S
authors.lastname: Sun
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cheng
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
authors.forename: Jianxun
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuanxi
authors.initials: Y
authors.lastname: Liao
authors.forename: Haoyang
authors.initials: H
authors.lastname: Wang
authors.forename: Yinlong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014026:Nicotiana / Q000737:chemistry*; D014835:Volatilization
chemical_list: D009930:Organic Chemicals