Modulation of cell proliferation and gene expression by alpha-tocopheryl phosphates: relevance to atherosclerosis and inflammation.
The effect of a mixture of alpha-tocopheryl phosphate plus di-alpha-tocopheryl phosphate (TPm) was studied in vitro on two cell lines, RASMC (from rat aortic smooth muscle) and human THP-1 monocytic leukemia cells. Inhibition of cell proliferation by TPm was shown in both lines and occurred with TPm at concentrations lower than those at which alpha-tocopherol was equally inhibitory. TPm led in nonstimulated THP-1 cells to inhibition of CD36 mRNA and protein expression, to inhibition of oxidized low-density lipoprotein surface binding and oxLDL uptake. In nonstimulated THP-1 cells, alpha-tocopherol had only very weak effects on these events.
Ogru
Esra
E
Department of Biochemistry and Molecular Biology, University of Bern, Buhlstrasse 28, Bern 3012, Switzerland. angelo.azzi@mci.unibe.ch
Libinaki
Roksan
R
Gianello
Robert
R
West
Simon
S
Munteanu
Adelina
A
Zingg
Jean-Marc
JM
Azzi
Angelo
A
D000818:Animals; D001011:Aorta; D017209:Apoptosis / Q000187:drug effects; D001161:Arteriosclerosis; D018955:CD36 Antigens / Q000235:genetics; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007249:Inflammation; D007938:Leukemia / Q000473:pathology; D008077:Lipoproteins, LDL / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D051381:Rats; D014407:Tumor Cells, Cultured; D024502:alpha-Tocopherol / Q000031:analogs & derivatives* / Q000494:pharmacology*
D018955:CD36 Antigens; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C012552:alpha-tocopherol phosphate; D024502:alpha-Tocopherol