title: Maintenance of neuronal positions in organized ganglia by SAX-7, a Caenorhabditis elegans homologue of L1.
abstract: The L1 family of cell adhesion molecules is predominantly expressed in the nervous system. Mutations in human L1 cause neuronal diseases such as HSAS, MASA, and SPG1. Here we show that sax-7 gene encodes an L1 homologue in Caenorhabditis elegans. In sax-7 mutants, the organization of ganglia and positioning of neurons are abnormal in the adult stage, but these abnormalities are not observed in early larval stage. Misplacement of neurons in sax-7 mutants is triggered by mechanical force linked to body movement. Short and long forms of SAX-7 exhibited strong and weak homophilic adhesion activities in in vitro aggregation assay, respectively, which correlated with their different activities in vivo. SAX-7 was localized on plasma membranes of neurons in vivo. Expression of SAX-7 only in a single neuron in sax-7 mutants cell-autonomously restored its normal neuronal position. Expression of SAX-7 in two different head neurons in sax-7 mutants led to the forced attachment of these neurons. We propose that both homophilic and heterophilic interactions of SAX-7 are essential for maintenance of neuronal positions in organized ganglia.
authors.affiliation: Department of Molecular Biology, Graduate School of Science, Nagoya University, Nagoya, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasakura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Inada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuhara
authors.forename: Eri
authors.initials: E
authors.lastname: Fusaoka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takemoto
authors.forename: Kosei
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Ikue
authors.initials: I
authors.lastname: Mori
mesh_terms: D000818:Animals; D016367:Baculoviridae; D015153:Blotting, Western; D012831:Bombyx; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002449:Cell Aggregation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D040461:Gene Components; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008565:Membrane Proteins / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D039842:Neural Cell Adhesion Molecule L1 / Q000235:genetics; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; C501528:SAX-7 protein, C elegans; D049452:Green Fluorescent Proteins