[Study on the molecular epidemiology of beta-lactamase TEM gene in isolated Streptococcus pneumoniae].
OBJECTIVE
To investigate the beta-lactamase TEM gene of isolated Streptococcus pneumoniae (Sp) in Suzhou area.


METHODS
Twenty-three strains of Sp were collected from respiratory tract secretions of children with respiratory diseases in Nov 2002 to Apr 2003 at Children's Hospital of Suzhou University (reference strain ATCC49619) to build TEM polymerase chain reaction (PCR) system (reference strain E. coli. 9-j53R1 with TEM gene) TEM gene of 23 strains was detected to comparo the sequences with published TEM gene sequences in GenBank for analyzing TEM gene model.


RESULTS
Twenty-one strains had TEM gene with a positive rate of 91.3% (21/23). TEM-129 gene were confirmed from No.17 (SR017, penicillin resistance) TEM sequence. New discovered TEM-129 sequence had a modification (ATG[M]-->ATA[I]) at No.182 code and published (GenBank: www.ncbi.nlm.nih.gov/nucleotide, AY452662). TEM-1 genes were confirmed from other TEM sequences. New discovered TEM-1 gene of isolated Sp had been published (GenBank: www.ncbi.nlm.nih.gov/nucleotide, AY392531) too.


CONCLUSIONS
Isolated Sp had TEM gene (TEM-129, EM-1 genotype) with a positive rate of 91.3%. The result enriched the understanding of isolated Sp with penicillin resistance.
Ding
Yun-fang
YF
Affiliated Children's Hospital of Suzhou University, Suzhou 215003, China.
Zhang
Jian-hua
JH
Mi
Zu-huang
ZH
Qin
Ling
L
Tao
Yun-zhen
YZ
Qi
Xiao
X
D001483:Base Sequence; D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D018410:Pneumonia, Bacterial / Q000453:epidemiology* / Q000235:genetics / Q000382:microbiology; D017354:Point Mutation; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000235:genetics; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases; C034413:beta-lactamase TEM-1