title: Polymorphisms in GSTT1 and p53 and urinary transitional cell carcinoma in south-western Taiwan: a preliminary study.
abstract: Little is known about the relevance of genetic polymorphisms to arsenic-related bladder cancer. A preliminary case-control study was conducted to explore the association between genetic polymorphisms of GSTT1, p53 codon 72 and bladder cancer in southern Taiwan, a former high arsenic exposure area. Fifty-nine urinary transitional cell carcinoma (TCC) patients from a referral centre in south-western Taiwan and 81 community controls matched on residence were recruited from 1996 to 1999. A questionnaire was administered to obtain arsenic exposure and general health information. Genotypes of p53 codon 72 and GSTT1 were analysed by polymerase chain reaction-restriction fragment length polymerase. The combined variant genotypes (heterozygous or homozygous variant) of p53 codon 72 and GSTT1 null were observed in 29% of cases and in 44% of controls, respectively. In this preliminary study, bladder cancer risk was slightly elevated for subjects carrying the variant genotype of p53 codon 72 or in subjects carrying the GSTT1 null genotype. Variants in p53 codon 72 increased the risk of bladder cancer among smokers. However, the results were not statistically significant and larger confirmatory studies are needed to clarify the role of candidate gene polymorphisms and bladder cancer risk in arsenic exposed populations.
authors.affiliation: Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, MA 02115, Boston, USA. karen.chen@channing.harvard.edu
authors.forename: Y-C
authors.initials: YC
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: Y-L L
authors.initials: YL
authors.lastname: Guo
authors.forename: H-J J
authors.initials: HJ
authors.lastname: Su
authors.forename: T J
authors.initials: TJ
authors.lastname: Smith
authors.forename: L M
authors.initials: LM
authors.lastname: Ryan
authors.forename: M-S
authors.initials: MS
authors.lastname: Lee
authors.forename: D C
authors.initials: DC
authors.lastname: Christiani
mesh_terms: D000328:Adult; D000368:Aged; D020261:Arsenic Poisoning / Q000150:complications*; D002295:Carcinoma, Transitional Cell / Q000209:etiology* / Q000235:genetics; D016022:Case-Control Studies; D003062:Codon; D005260:Female; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking; D013624:Taiwan; D001749:Urinary Bladder Neoplasms / Q000209:etiology* / Q000235:genetics
chemical_list: D003062:Codon; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase