IgE-binding abilities of pentapeptides, QQPFP and PQQPF, in wheat gliadin.
Wheat gliadin is known mainly as the causative substance for enteropathy accompanied by diarrhea (celiac disease) and food-dependent exercise-induced anaphylaxis. However, little is known in regards to the allergenicity of gliadin in atopic dermatitis. In this study, the allergenicity of gliadin was demonstrated using sera of wheat-allergic patients with atopic dermatitis. Since there are many repeated sequences in gliadin, peptides containing each motif were synthesized to evaluate their IgE-binding abilities. As a result, QQPFP and PQQPF were found to be epitopes (minimum structures for the IgE-binding). Gliadin might crosslink patient's IgE-antibodies via these epitopes and trigger subsequent allergic reactions.
Tanabe
Soichi
S
Faculty of Education, Tokyo Gakugei University, Koganei 184-8501, Japan. stanabe@hiroshima-u.ac.jp
D000485:Allergens / Q000737:chemistry / Q000276:immunology; D000595:Amino Acid Sequence; D003876:Dermatitis, Atopic / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology; D005903:Gliadin / Q000737:chemistry / Q000276:immunology*; D005983:Glutens / Q000031:analogs & derivatives* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D014908:Triticum / Q000276:immunology*
D000485:Allergens; D000939:Epitopes; D009842:Oligopeptides; D010446:Peptide Fragments; D007073:Immunoglobulin E; D005983:Glutens; D005903:Gliadin; C037302:glutenin