-374T/A polymorphism of the RAGE gene promoter in relation to severity of coronary atherosclerosis.
BACKGROUND
We have recently reported that homozygosity for the minor A-allele of RAGE (receptor for advanced glycation end products) -374T/A polymorphism may exert a protective effect toward the development of angiographic coronary artery disease (CAD). Here we focused on the putative involvement of this functional RAGE polymorphism on the severity of coronary atherosclerosis as assessed by angiography.


METHODS
In a total of 234 consecutive Caucasian patients with angiographically proven CAD, the severity of coronary atherosclerosis was assessed by the number of diseased vessels (greater than 50% stenosis). Genotyping for the -374T/A variant was performed by means of PCR-RFLPs.


RESULTS
The mean number of diseased vessels was significantly lower in patients with the AA genotype (1.47+/-0.68) than in those with the AT or TT genotype (1.88+/-0.82, p=0.029). After confounding variables were controlled for, the number of diseased vessels remained significantly different in the AA genotype carriers from that in the AT or TT carriers (p=0.041, ANCOVA).


CONCLUSIONS
Our data suggest that the RAGE -374T/A polymorphism is one of the likely candidate determinants for the genetic variance of disease phenotype in coronary atherosclerosis.
Falcone
Colomba
C
Department of Hematological, Pneumological and Cardiovascular Sciences, Division of Cardiology, University Hospital IRCCS Policlinico San Matteo, University of Pavia, Piazzale Golgi 2, 27100 Pavia, Italy. c.falcone@smatteo.pv.it
Campo
Ilaria
I
Emanuele
Enzo
E
Buzzi
Maria P
MP
Geroldi
Diego
D
Belvito
Chiara
C
Zorzetto
Michele
M
Sbarsi
Ilaria
I
Cuccia
Mariaclara
M
D000241:Adenosine / Q000235:genetics*; D000483:Alleles; D003324:Coronary Artery Disease / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics*; D012720:Severity of Illness Index; D013936:Thymidine / Q000235:genetics*; D044465:White People / Q000235:genetics
D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D000241:Adenosine; D013936:Thymidine