title: Helicobacter pylori-dependent NF-kappa B activation in newly established Mongolian gerbil gastric cancer cell lines.
abstract: Mongolian gerbils are an ideal animal model to explore the role of H. pylori on cancer development. However, there have been no established adenocarcinoma cell lines from this model animal. In the present study, we have established cancer cell lines from a primary gastric cancer tissue of a Mongolian gerbil. The derived cells could be stably attached with H. pylori, revealed under a scanning electron microscope, and easily transplanted to the nude mice. Rapid phosphorylation of IkappaB, Erk1/2, and AKT of these cells was observed by Interleukin-1 beta stimulation, and luciferase reporter gene assay on transcriptional activation of Nuclear Factor kappa B after challenging with either H. pylori NCTC11637 or its isogenic cagE-knockout mutant, H. pylori revealed the cagE-dependent NF-kappaB transcriptional activation. The newly established cancer cell lines from the in vivo gastric carcinogenesis model animal, the Mongolian gerbil, can be used to develop effective therapeutic strategies against gastric cancer, especially in exploring the effect of H. pylori, and thus might greatly contribute to gastric cancer prevention and treatment in humans.
authors.affiliation: Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya, Aichi 464-8681, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nozaki
authors.forename: Harunari
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ikehara
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Cao
authors.forename: Hayao
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Azuma
authors.forename: Shiho
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shimizu
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Mafune
authors.forename: Michio
authors.initials: M
authors.lastname: Kaminishi
authors.forename: Masae
authors.initials: M
authors.lastname: Tatematsu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000472:pathogenicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology* / Q000662:veterinary; D014407:Tumor Cells, Cultured
chemical_list: D016328:NF-kappa B