[Characteristics of municipal sewage chem-bioflocculation treatment process by using PCR-DGGE technology].
It is reported that without cultivation, DNA could be directly extracted from environmental samples with molecular biological methods, such as polymerase chain reaction (PCR) and denaturting gradient gel electrophoresis (DGGE). To analyze the community diversity of activated sludge and bio-film in the municipal sewage, work was done to directly extrude crude DNA from activated sludge and bio-film samples, separate and amplify 16S rDNA by PCR and sequence it with DGGE. The results show the significant microbe community difference between cultivated and uncultivated activated sludge. Further research on the community diversity of two different sewage treatment processes was done and initial discussion on the microbial distribution in the same reactor and microbial structure in different experimental conditions was carried out. The sequences of several 16S rDNA DGGE fragments were determined and some possible bacteria were confirmed in comparision in GeneBank (NCBI). The results show that the PCR-DGGE technology combined with sequences determination is a feasible and efficient method for microorganism analysis in environmental sample.
Wang
Feng
F
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Fu
Yi-gang
YG
Xia
Si-qing
SQ
Yang
Dian-hai
DH
D001673:Biodegradation, Environmental; D019149:Bioreactors; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005427:Flocculation; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012722:Sewage; D014865:Waste Disposal, Fluid / Q000379:methods*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage