Involvement of Disabled-2 protein in the central nervous system inflammation following experimental cryoinjury of rat brains.
Disabled-2 (Dab-2) functions in the mitogenic signal transduction pathway, and is expressed in a variety of tissues. We investigated the roles of Dab-2 expression in the rat brain following experimental cryoinjury in relation to central nervous system (CNS) inflammation. Western blot analysis showed that Dab-2 expression increased significantly (p < 0.001) in the frontal cortex 4-14 days after cryoinjury, and declined slightly thereafter. Immunohistochemistry showed that Dab-2 immunostaining occurred in most of the vessels in the control cerebral cortex. After cryoinjury, Dab-2 was localized in the majority of inflammatory cells (especially in ED1-positive macrophages) in the core and periphery, as well as in vessels. These findings suggest that Dab-2 is involved in the inflammation that follows CNS injury through the migration of activated inflammatory cells in the rat brain.
Moon
Changjong
C
Department of Veterinary Medicine, Cheju National University, Jeju 690-756, South Korea.
Lee
Jeeyoung
J
Ahn
Meejung
M
Shin
Taekyun
T
D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001930:Brain Injuries / Q000150:complications / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D003080:Cold Temperature / Q000009:adverse effects; D004195:Disease Models, Animal; D053331:Ectodysplasins; D004660:Encephalitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005625:Frontal Lobe / Q000378:metabolism / Q000473:pathology; D007839:Functional Laterality; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D033942:Adaptor Proteins, Vesicular Transport; C501404:Dab2 protein, rat; D053331:Ectodysplasins; D005904:Glial Fibrillary Acidic Protein; D008565:Membrane Proteins