The Chinese traditional medicine 'Bushen Yinao Pian' increased the level of ageing-related gene LRPAP-1 expression in the cerebral tissue of accelerated senescence-prone mouse 8/Ta.
The molecular mechanism of the Chinese traditional medicine 'Bushen Yinao Pian' (a complex prescription used for clinical anti-ageing in China for over 20 years) is elusive. In this study, the cDNA of low-density lipoprotein related-receptor associated protein-1 (LRPAP-1), an ageing-related gene, which functions as a chaperon or escort protein in the intracellular transport of low-density lipoprotein related-receptor, a transporter of amyloid beta protein (AbetaP), had been cloned by screening cDNA library based on analyzing the gene expression in cerebral tissue between the test and the control accelerated senescence-prone mouse 8/Ta (SAMP8/Ta). The result shows that this complex prescription increased the expression level of LRPAP-1. It indicated that the Chinese traditional medicine 'Bushen Yinao Pian' plays an important role in anti-ageing by increasing LRPAP-1 expression level.
Zhang
Chong
C
The Institute of Genetics and Developmental Biology, Chinese Academy of sciences, P.O. Box 2707, Beijing 100080, China.
Yang
Ting
T
Wang
Jingang
J
Liu
Guisheng
G
Chen
Qingxuan
Q
D000284:Administration, Oral; D019588:Aging, Premature / Q000235:genetics*; D000818:Animals; D001927:Brain Diseases / Q000188:drug therapy / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D015723:Gene Library; D026563:LDL-Receptor Related Protein-Associated Protein / Q000235:genetics; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004365:Drugs, Chinese Herbal; D026563:LDL-Receptor Related Protein-Associated Protein; D018832:Molecular Chaperones; D028321:Plant Preparations; D012333:RNA, Messenger; C506673:bushen yinao pian