title: [Determination of deoxyribonuceic acids by resonance light scattering technique with benzidine in the presence of the sensitizing sodium dodecyl sulfate].
abstract: The resonance light scattering (RLS) spectra of benzidine with deoxyribonucleic acid (ctDNA) in the presence of Sodium Dodecyl Sulfate had been studied. RLS intensity of SDS-benzidine system was enhanced by trace ctDNA and was proportional to the concentrationof ctDNA. Under optima conditions, the linear ranges of the calibration were 0.3-4 mg x L(-1), the linear regression equation was I = 37.17c (ctDNA, mg x L(-1)) + 208.9, r = 0.9992. The limit of detection were 0.17 mg x L(-1) (3delta), RSD% = 3.5%. The method had been applied to the determination of DNA in synthetic samples and gene tobacco sample, and the results were satisfactory.
authors.affiliation: College of Science, China Agricultural University, Beijing 100094, China.
authors.forename: Zhen-hong
authors.initials: ZH
authors.lastname: Rao
authors.forename: Yan-mei
authors.initials: YM
authors.lastname: Xiong
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Ren
authors.forename: Shu-ren
authors.initials: SR
authors.lastname: Jiang
authors.forename: Chun-rong
authors.initials: CR
authors.lastname: Zhang
mesh_terms: D001560:Benzidines / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D008027:Light; D057230:Limit of Detection; D012542:Scattering, Radiation; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D001560:Benzidines; C029876:benzidine; D012967:Sodium Dodecyl Sulfate; D004247:DNA