Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov.
An aerobic, motile, Gram-positive strain, DX3-12(T), was isolated from coastal sediment of the Eastern China Sea in Fujian Province, China, and was identified by means of polyphasic taxonomy. On the basis of 16S rRNA gene sequence similarity, strain DX3-12(T) was closely related to members of the genera Planomicrobium and Planococcus. 16S rRNA gene sequence similarities between strain DX3-12(T) and Planomicrobium mcmeekinii, Planomicrobium koreense, Planomicrobium okeanokoites, Planococcus psychrophilus and Planococcus alkanoclasticus were 97.8, 97.8, 97.1, 97.5 and 96.4 %, respectively. Chemotaxonomic and physiological properties of strain DX3-12(T) supported its affiliation to the genus Planomicrobium. Strain DX3-12(T) and Planomicrobium mcmeekinii exhibited common phenotypic properties such as the ability to reduce nitrate, but they could be distinguished from each other by physiological and biochemical tests, e.g. acid production from glucose, growth temperature and NaCl concentration requirements. DNA-DNA hybridization further distinguished strain DX3-12(T) from Planomicrobium mcmeekinii at the species level. Therefore, it is proposed that strain DX3-12(T) (=AS 1.3454(T)=JCM 12466(T)) represents a novel species, Planomicrobium chinense sp. nov. Based on 16S rRNA gene sequence similarities, signature nucleotides, cell morphology and physiological and biochemical properties, Planococcus psychrophilus and Planococcus alkanoclasticus have been transferred to the genus Planomicrobium as Planomicrobium psychrophilum comb. nov. (type strain DSM 14507(T)=MTCC 3812(T)) and Planomicrobium alkanoclasticum comb. nov. (type strain NCIMB 13489(T)=CIP 107718(T)), respectively.
Dai
Xin
X
State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, Zhong-Guan-Cun, Haidian, Beijing 100080, PR China.
Wang
Ya-Nan
YN
State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, Zhong-Guan-Cun, Haidian, Beijing 100080, PR China.
Wang
Bao-Jun
BJ
State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, Zhong-Guan-Cun, Haidian, Beijing 100080, PR China.
Liu
Shuang-Jiang
SJ
State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, Zhong-Guan-Cun, Haidian, Beijing 100080, PR China.
Zhou
Yu-Guang
YG
State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, Zhong-Guan-Cun, Haidian, Beijing 100080, PR China.
D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S