Identification of cell lineages involved by t(15;17) in acute promyelocytic leukemia by combined fluorescence activated cell sorting and FISH.
Bone marrow cells from five patients with acute promyelocytic leukemia (APL) with t(15;17) were studied by a combination of fluorescence activated cell sorting and fluorescence in situ hybridization (FISH) to establish the cell lineage involvement of t(15;17). Interphase FISH demonstrated that the fusion gene (PML/RARA) was present in almost all abnormal promyelocytes. In one case, the translocation was demonstrated in both CD34+ and CD34- APL cells. The t(15;17) abnormality was not detectable in erythroblasts nor in T- or B-lymphoid cells. These results suggest that lymphocytes and erythroblasts are not clonally involved in APL, and that malignant transformation in some cases of APL may occur at the level of CD34+ cells.
Chang
Hong
H
Department of Laboratory Hematology, Princess Margaret Hospital/University Health Network, University of Toronto, 610 University Avenue, 4-320, Toronto, Ontario M5G-2M9, Canada. hong.chang@uhn.on.ca
Qi
Xiao Ying
XY
Sutherland
D Robert
DR
D000328:Adult; D019070:Cell Lineage; D002469:Cell Separation; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014178:Translocation, Genetic