title: Characterization and expression analysis of CD3varepsilon and CD3gamma/delta in fugu, Takifugu rubripes.
abstract: CD3 is an essential component of the CD3-TCR complex. In this report, we describe the cloning, characterization, and expression analysis of the CD3varepsilon and CD3gamma/delta chain genes from fugu, Takifugu rubripes. Two distinct CD3varepsilon homologue cDNAs, designated as CD3varepsilon-1 and CD3varepsilon-2, and a CD3gamma/delta homologue cDNA were isolated from the fugu thymus. The deduced amino acid sequences of these cDNAs exhibit conserved essential CD3 chain motifs and overall structures. RT-PCR analysis demonstrated that the CD3varepsilon and CD3gamma/delta genes were expressed in lymphoid organs (e.g. thymus, head kidney, trunk kidney and spleen), mucosal tissues (gill, skin, and intestine), and peripheral blood leucocytes (PBL). The CD3 and TCRalpha genes were expressed only in the surface IgM- population, which were separated from PBL using an anti-fugu IgM monoclonal antibody. In addition, in situ hybridization confirmed that CD3-expressing cells were distributed randomly in the head kidney, trunk kidney, and spleen, but in the thymus were restricted to the lymphoid outer zone and epithelioid inner zone only. Collectively, these results suggest that CD3 molecules are useful markers for the identification of T cells in teleost fish. The present study thus provides a critical step in identifying T cells in this model organism.
authors.affiliation: Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 2971-4 Maisaka, Shizuoka, 431-0211, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Araki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suetake
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001483:Base Sequence; D017252:CD3 Complex / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D023741:Takifugu / Q000235:genetics / Q000276:immunology*
chemical_list: D017252:CD3 Complex; C452842:CD3 antigen, gamma chain; C472562:CD3delta antigen; D011948:Receptors, Antigen, T-Cell