Trypanosoma cruzi: molecular characterization of TcPUF6, a Pumilio protein.
In trypanosomes regulation of gene expression occurs mainly at the post-transcriptional level. Pumilio proteins are RNA-binding proteins that modulate gene expression in lower and higher eukaryotes. Here we present the characterization of TcPUF6, a member of the Pumilio family in Trypanosoma cruzi. TcPUF6 is expressed in the different life cycle forms of the parasite showing no clear stage specific regulation and it is localized to multiple discrete foci in the cytoplasm of epimastigotes. The recombinant TcPUF6 fusion protein specifically binds to the Drosophila hunchback NRE (nanos response element). TcPUF6 conserves functional properties that characterize the Pumilio family throughout evolution.
Dallagiovanna
Bruno
B
Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay.
Pérez
Leticia
L
Sotelo-Silveira
José
J
Smircich
Pablo
P
Duhagon
María Ana
MA
Garat
Beatriz
B
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D016053:RNA, Protozoan / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000235:genetics; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000235:genetics*
D015800:Protozoan Proteins; D016053:RNA, Protozoan; D016601:RNA-Binding Proteins