title: Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV.
abstract: A HPLC-UV method for the quantification of six major isoflavonoids, calycosin 7-O-beta-D-glucoside (1), formononetin 7-O-beta-D-glucoside (2), (6alphaR, 11alphaR) 3-hydroxy-9,10-dimethoxypterocarpan-3-O-beta-D-glucoside (3), 7,2'-dihydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside (4), calycosin (5) and formononetin (6), in Radix Astragali (Huangqi) was developed and validated. The method was proven to be sensitive, specific, accurate and precise, as well as effective and easy.
authors.affiliation: Shanghai R&D Center for Standardization of Traditional Chinese Medicines, Shanghai, 201203, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: S W
authors.initials: SW
authors.lastname: Annie Bligh
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Gu
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: He-ping
authors.initials: HP
authors.lastname: Liu
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Cheng
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Branford-White
authors.forename: Zhi-bi
authors.initials: ZB
authors.lastname: Hu
mesh_terms: D027883:Astragalus Plant; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007529:Isoflavones / Q000737:chemistry*; D008517:Phytotherapy; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007529:Isoflavones