[Effect of cytomegalovirus strain selection on stimulation of cytokine panel Th1/Th2].
CMV infection can undergoing as primary or secondary infection, which can be represented by reactivation of endogenous infection or by superinfection with another viral strain. Taking for consideration high prevalence of seropositive persons it may be concluded that superinfections occur quite often. For the other hand it is known, that differences among CMV strains are deep and regard as well viral structure as its biological properties. The aim of this study was investigation of immunological profile in range of cytokines production after stimulation with different CMV strains. The study was conducted with leukocytes obtained from CMV seropositive person cultivated and stimulated in vitro with AD169, Towne and Davis. After 24, 48, 72 and 96 hours incubation concentrations of IL-2, IL-4, IL-5, IL-10, INF-gamma and TNF-alpha in supernatants was measured by flow cytometry with Cytometric Bead Array technique. Obtained results allow to conclude that difference in level of stimulated cytokines and their panel is dependent on viral strain used. The experiment also allows to optimisation of stimulation conditions.
Siennicka
Joanna
J
Zakład Wirusologii PZH w Warszawie. jsiennicka@pzh.gov.pl
Gut
Włodzimierz
W
Litwińska
Bogumiła
B
D000914:Antibodies, Viral / Q000097:blood; D016207:Cytokines / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D018417:Th1 Cells / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*; D013997:Time Factors
D000914:Antibodies, Viral; D016207:Cytokines; D007074:Immunoglobulin G