Investigations of the transfructosylation reaction by fructosyltransferase from B. subtilis NCIMB 11871 for the synthesis of the sucrose analogue galactosyl-fructoside.
The exo-fructosyltransferase produced from B. subtilis NCIMB 11871 strain transfers the fructose moiety from donor alpha12 linked saccharides such as sucrose, raffinose and stachyose to the acceptor d-galactose, leading to the sucrose analogue, galactosyl-fructoside. Here, we report detailed kinetic studies. The enzyme showed a remarkably high optimal temperature at 50 degrees C and was effectively immobilised on Eupergit C 250 L and Trisopor-Amino. This is also the first report about the equilibrium of the transfructosylation reaction, its activation energy determination, the structure of the product and its preparative scale isolation.
Baciu
I-E
IE
Technical Chemistry, Department for Carbohydrate Technology, Technical University Braunschweig, Langer Kamp 5, D-38106 Braunschweig, Germany.
Jördening
H-J
HJ
Seibel
J
J
Buchholz
K
K
D001412:Bacillus subtilis / Q000145:classification / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005632:Fructose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006602:Hexosyltransferases / Q000737:chemistry*; D013045:Species Specificity; D013395:Sucrose / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D013696:Temperature; D001426:Bacterial Proteins
D004800:Enzymes, Immobilized; D005632:Fructose; D005947:Glucose; D006602:Hexosyltransferases; D013395:Sucrose; C021451:inulosucrase; D001426:Bacterial Proteins