Histological study of the effect of some irrigating solutions on bacterial endotoxin in dogs.
The aim of this study was to evaluate, histopathologically, the effectiveness of mechanical preparation of root canals using different irrigating solutions in dog teeth filled with LPS after pulpectomy. A total of 120 root canals of 6 mongrel dogs were filled with a solution of LPS after pulpectomy. The irrigating solutions used were saline, 1, 2.5, and 5% sodium hypochlorite, and 2% chlorhexidine. No irrigation was used in the control group. The animals were sacrificed after 60 days and the teeth were fixed and demineralized. Subsequently, serial 6-microm sections were stained with hematoxylin and eosin and Mallory's trichrome for histopathological analysis and Brown-Brenn for verification of bacterial contamination. Analysis showed that the inflammatory infiltrate was statistically less intense in the groups in which the root canals were irrigated with 5% sodium hypochlorite and 2% chlorhexidine. However, none of the irrigating solutions completely inactivated the harmful effects of LPS. Mechanical preparation associated with different irrigating solutions did not completely inactivate LPS.
Silva
Léa Assed Bezerra da
LA
Department of Clinical Pediatrics, School of Dentistry of Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil. msantos@forp.usp.br
Leonardo
Mario Roberto
MR
Assed
Sada
S
Tanomaru Filho
Mário
M
D000818:Animals; D002710:Chlorhexidine / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000187:drug effects; D004285:Dogs; D005260:Female; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008297:Male; D010486:Periapical Tissue / Q000187:drug effects; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation; D012965:Sodium Chloride / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric
D008070:Lipopolysaccharides; D012388:Root Canal Irrigants; D012965:Sodium Chloride; D012973:Sodium Hypochlorite; D002710:Chlorhexidine