title: Acute pulmonary inflammation induced by lung overloading with selenium particles: leukocyte response and in situ detection of selenium at high resolution.
abstract: The kinetics of the acute inflammatory response of the lung was triggered in CD-1 mice by a single intratracheal instillation of a large amount of Se (10 mg); it was studied by quantitative cytology of bronchoalveolar lavage samples, light microscopy, and scanning electron microscopy coupled with x-ray elemental microanalysis. Bronchoalveolar lavage leukocytes were mostly neutrophils and increased from 12 to 24 h of Se treatment and decreased at 72 h. Only less than half of the granulocytes showed ingested Se particles; in contrast, virtually all BAL macrophages contained Se particles. Scanning electron microscopy coupled with X-ray elemental microanalysis revealed that the intracellular Se particles were heterogeneous in size (diameters from 0.4 and up to 14 microm), and that Se inclusions were sometimes accumulated at a pole of the cell. At 72 h after instillation of the particles, Se-loaded alveolar macrophages were migrated in the interstitial space of the alveoli. Se-positive regions had a focal distribution in the lung; accumulation of inflammatory cells erased the alveolar architecture of these areas of the deep lung. Our data indicates that Se overloading of the lung results in: (1) an acute inflammatory response that is dominated by neutrophils; (2) early removal of Se done mostly by alveolar macrophages, and (3) formation of focal areas of invasion of the lung parenchyma by inflammatory infiltrates.
authors.affiliation: ICBAS, Department of Anatomy and UMIB, Abel Salazar Institute for Biomedical Sciences, University of Porto, Porto, Portugal, European Union. duangrud@yahoo.com
authors.forename: Duangrudee
authors.initials: D
authors.lastname: Cherdwongcharoensuk
authors.forename: Suchart
authors.initials: S
authors.lastname: Upatham
authors.forename: António Sousa
authors.initials: AS
authors.lastname: Pereira
authors.forename: Artur P
authors.initials: AP
authors.lastname: Aguas
mesh_terms: D000208:Acute Disease; D000818:Animals; D018893:Bronchoalveolar Lavage; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D004577:Electron Probe Microanalysis / Q000379:methods; D005260:Female; D002479:Inclusion Bodies / Q000737:chemistry / Q000648:ultrastructure; D007442:Intubation, Intratracheal; D008168:Lung / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D010316:Particle Size; D011014:Pneumonia / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D012643:Selenium / Q000008:administration & dosage / Q000032:analysis* / Q000633:toxicity*
chemical_list: D012643:Selenium