Administration of myo-inositol plus ethanolamine elevates phosphatidylethanolamine plasmalogen in the rat cerebellum.
Plasmalogens are ether-linked phospholipids highly abundant in nervous tissue. Previously we demonstrated that acute administration of myo-inositol (myo-Ins) + [2-(13)C] ethanolamine ([2-(13)C]Etn) significantly elevated phosphatidylethanolamine plasmalogen (PlsEtn) in rat whole brain. Current experiments investigated the effects of acute myo-Ins+[2-(13)C]Etn administration on [PlsEtn] and the biosynthesis of new Etn lipids using NMR spectroscopy in rat cerebral cortex, hippocampus, brainstem, midbrain and cerebellum. Treated rats received a single dose of myo-Ins + [2-(13)C]Etn and controls received saline rather than myoIns. Data reveal that the cerebellum is the brain region most affected by treatment, which resulted in a 22% increase in [PlsEtn] and 89% increase in newly synthesized Etn lipids relative to controls (P < 0.05). Furthermore, the cerebellar PlsEtn/phosphatidylethanolamine ratio and molar percentage of PlsEtn were significantly elevated by 12% and 8%, respectively (P < 0.05). These data suggest that myo-Ins influences Etn lipid metabolism in brain, particularly in the cerebellum where there is a stimulation in the biosynthesis of new Etn lipids with a preference towards PlsEtn.
Hoffman-Kuczynski
Beth
B
Department of Biochemistry and Molecular Biology, Wright State University School of Medicine, WSU Magnetic Resonance Laboratory, Cox Institute, Dayton, Ohio 45429, USA.
Reo
Nicholas V
NV
D000818:Animals; D002531:Cerebellum / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004338:Drug Combinations; D019856:Ethanolamine / Q000008:administration & dosage*; D007294:Inositol / Q000008:administration & dosage*; D008297:Male; D010955:Plasmalogens / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D011916:Rats, Inbred F344
D004338:Drug Combinations; D010955:Plasmalogens; C020791:phosphatidal ethanolamines; D007294:Inositol; D019856:Ethanolamine