Characterizing CD43 expression in haematogones using multicolor flow cytometric analysis.
Haematogones are precursor B cells commonly detected in small numbers in the bone marrow. Morphologically, haematogones can mimic lymphoblasts and are best distinguished using multicolour flow cytometry with antibody combinations. Haematogones show characteristic and reproducible patterns of antigen expression representing the B-cell maturation sequence. CD43 expression, widely seen in haematopoietic elements, has not been well characterized in haematogones. We demonstrate that CD43 is consistently expressed in haematogones in a reproducible pattern similar to that of CD10 when combined with CD20. We propose that in combination with other markers, CD43 can be useful in the identification of haematogones.
Tsao
Lawrence
L
Department of Pathology, Columbia University, New York, NY 10032, USA.
Colovai
Adriana I
AI
Jiang
Jie-Gen
JG
Bhagat
Govind
G
Alobeid
Bachir
B
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism*; D018941:Antigens, CD19 / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007223:Infant; D051917:Leukosialin; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism*
D015703:Antigens, CD; D018941:Antigens, CD19; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins; D015260:Neprilysin