[Spectroscopic evaluation of time-resolved fluoroimmunoassay].
The lanthanide trivalence ion and its chelates are used for marking substance in Time-Resolved Fluorescence Immunoassay (TRFIA), marking protein, hormone, antibody, nucleic acid probe or biologic alive cell, to measure the concentration of the analysis substance inside the reaction system with time-resolved fluorometry after the reaction system occurred, and attain the quantitative analysis's purpose. TRFIA has therefore become a kind of new and more sensitive measurement method after radioisotope marking, enzymatic marking, chemiluminescence, electrochemiluminescence, primarily depending on the special physics and chemistry characteristics of lanthanide trivalence ion and its chelates. In this paper, the result of spectroscopic evaluation of europium trivalence ion and its chelate, and the principle of time-resolved technology and fluorescence-enhanced technology are reported. At the same time, the experiment shows that the excitation wavelength chosen between 336 and 337 nm benefits the excitation and the energy transfer of chelate diketone of europium trivalence ion.
Guo
Zhou-yi
ZY
Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China.
Tian
Zhen
Z
Jia
Ya-li
YL
D005063:Europium / Q000737:chemistry*; D015200:Fluoroimmunoassay / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D049449:Luminescence; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013057:Spectrum Analysis; D013997:Time Factors
D007074:Immunoglobulin G; D005063:Europium