[Inactivation and sensitization of the tumor cells by the gene Bax transfection].
After the transfection of the gene Bax into the cultured tumor cells of human ovary adenocarcinoma SKOV3 and uterus carcinoma HeLa in vitro the high sensitivity of the cells SKOV3 to the protein Bax produced after the gene Bax transfection was found. The sensitivity of the cells HeLa to the gene Bax transfection was much smaller. The hyperexpression of gene Bax and hypersensitivity to doxorubicin were seen in HeLa cells received as a result of the gene Bax transfection and subsequent selection. All cells of the line SKOV3 with the increased expression of the transfected gene Bax died. In the cell line SKOV3 the mutation in a gene Bax was found which has a genotype G7/G9 against a native type of a gene Bax--G8/G8. It was concluded that the found in the exone 3 of the gene Bax mutation G7/G9 in cells SKOV3 results in an inactivation of proapoptotic activity of the protein Bax.
Rodina
A V
AV
Sladkova
L V
LV
Obukhova
V V
VV
Vezirkhanova
T Z
TZ
Moskaleva
E Iu
EIu
Prusakova
O V
OV
Beletskiĭ
I P
IP
Belushkina
N N
NN
Strel'nikov
V V
VV
Ivanov
M A
MA
Severin
S E
SE
Severin
E S
ES
D000230:Adenocarcinoma; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis; D002470:Cell Survival; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005260:Female; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008081:Liposomes; D009154:Mutation; D010051:Ovarian Neoplasms; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms; D051028:bcl-2-Associated X Protein
D000903:Antibiotics, Antineoplastic; C494826:BAX protein, human; D008081:Liposomes; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D049452:Green Fluorescent Proteins; D004317:Doxorubicin