Highly sensitive near-infrared fluorescent probes for nitric oxide and their application to isolated organs.
Novel near-infrared (NIR) fluorescent probes for nitric oxide (NO) have been designed, synthesized, and evaluated. Their NIR fluorescence was increased in an NO concentration-dependent manner under physiological conditions, and their reaction efficiency with NO was at least 53 times higher than that of a widely used NO probe, DAF-2. They were confirmed to function in isolated intact rat kidneys. Because NIR light can penetrate deeply into tissues, these probes may have potential for in vivo NO imaging.
Sasaki
Eita
E
Graduate School of Pharmaceutical Sciences, and Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Kojima
Hirotatsu
H
Nishimatsu
Hiroaki
H
Urano
Yasuteru
Y
Kikuchi
Kazuya
K
Hirata
Yasunobu
Y
Nagano
Tetsuo
T
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D010655:Phenylenediamines / Q000737:chemistry*; D051381:Rats; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000379:methods
C111205:4,5-diaminofluorescein; D002232:Carbocyanines; D005456:Fluorescent Dyes; D010655:Phenylenediamines; D009569:Nitric Oxide; C034193:1,2-diaminobenzene; D019793:Fluorescein