title: Analysis of gene expression induced by diethylstilbestrol (DES) in human primitive Mullerian duct cells using microarray.
abstract: The Mullerian ducts are strongly influenced by natural estrogen, estradiol (E2) and diethylstilbestrol (DES) in their development. We screened E2 and DES responsive genes using a microarray analysis in human primitive Mullerian duct cell line, EMTOKA cells expressed estrogen receptor (ER) beta. c-myc oncogene and other target genes expression was detected in cells treated by high-dose DES, but ER antagonist ICI 182,780 could not prevent c-myc induction above. Results of our present study suggested the presence of ER independent pathway in oncogenes induction process by high-dose DES treatment in a human primitive Mullerian duct cell line.
authors.affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi Aoba-ku, Sendai, Miyagi 980-8575, Japan. miki@patholo2.med.tohoku.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Miki
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Chika
authors.initials: C
authors.lastname: Tazawa
authors.forename: Masato
authors.initials: M
authors.lastname: Ishizuka
authors.forename: Shuho
authors.initials: S
authors.lastname: Semba
authors.forename: Itsuo
authors.initials: I
authors.lastname: Gorai
authors.forename: Hironobu
authors.initials: H
authors.lastname: Sasano
mesh_terms: D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D018929:Cell Culture Techniques; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D020869:Gene Expression Profiling; D016259:Genes, myc; D006801:Humans; D009095:Mullerian Ducts / Q000187:drug effects* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014594:Uterine Neoplasms / Q000139:chemically induced / Q000235:genetics; D014625:Vaginal Neoplasms / Q000139:chemically induced / Q000235:genetics
chemical_list: D018931:Antineoplastic Agents, Hormonal; D011960:Receptors, Estrogen; D004958:Estradiol; D004054:Diethylstilbestrol