[Expression of survivin in activated T cells and its significance].
OBJECTIVE
To investigate the expression of survivin in activated T cells in vitro and in vivo.


METHODS
Expressions of survivin and proliferating cell nuclear antigen (PCNA) in splenocytes stimulated with ConA or alloantigen were detected by immunohistochemical straining. Flow cytometry was used to analyze CD3, CD25 and survivin expressions and apoptosis of cultured splenocytes. Splenocytes from C57BL/6(H-2b) mice were infused into BALB/c(H-2d) nude mice to observe the survivin expression in vivo after alloantigen stimulation.


RESULTS
Expressions of survivin and PCNA were detected in stimulated cells. Survivin+ cells were CD3+ T cells, and lymphoblasts could simultaneously express CD3, CD25 and survivin. However, not all survivin+ cells expressed CD25. Five days after culture, apoptotic rate of ConA-stimulated splenocytes increased markedly (P<0.001). From day 4 to 7 after infusion, the alloantigen-activated T cell infiltrating in livers of nude mice expressed survivin.


CONCLUSIONS
Survivin can be detected in T cells after in vivo and in vitro stimulation, and thus may be used as a marker of activated T cells. Continuous expression of survivin in T cells needs continuous stimulation with alloantigen in vivo.
Chen
Bi-cheng
BC
Key Laboratory of Organ Transplantation Ministry of Education, Institute of Organ Transplantation Tongji Hospital, Tongji Medical College Huazhong University of Sciences and Technology, Wuhan 430030, China.
Tang
Li
L
Zhang
Xin
X
Guo
Hui
H
Chang
Sheng
S
Du
Dun-feng
DF
Chen
Zhong-hua
ZH
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002453:Cell Cycle / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007700:Kinetics; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D013154:Spleen / Q000166:cytology; D000077022:Survivin; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D013997:Time Factors
C117749:Birc5 protein, mouse; D017252:CD3 Complex; D051034:Inhibitor of Apoptosis Proteins; D053645:Interleukin-2 Receptor alpha Subunit; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D000077022:Survivin