title: [The inhibitory effect of deoxynivalenol on TAP-1 expression in human peripheral blood mononuclear cells in vitro].
abstract: OBJECTIVE
  To explore the effects of deoxynivalenol (DON) on transporter associated with antigen processing-1 (TAP-1) expression in human peripheral blood mononuclear cells (PBMCs).
  
  
  METHODS
  Effects of various concentrations of DON on TAP-1 expression of in vitro cultured human PBMCs and the dose-effect relationship were analyzed by flow cytometry (FCM) and semi-quantitative RT-PCR at the protein and mRNA levels.
  
  
  RESULTS
  FCM analysis indicated that treatment with various concentrations of DON could inhibit TAP-1 expressions in PBMCs, showing a negative correlation between DON concentration and TAP-1 expression. Semi-quantitative RT-PCR detection indicated that high concentrations of DON (1,000 and 2,000 microg/L) could distinctly inhibit TAP-1 mRNA expression.
  
  
  CONCLUSIONS
  DON can down-regulate TAP-1 expression in human PBMCs in a dose-dependent manner in vitro, which suggests DON may interfere with host immunosurveillance, and this may account for the correlation between DON-contaminated grain and esophagus cancer in high risk area.
authors.affiliation: Lab.of Experiment Pathology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Yue-hong
authors.initials: YH
authors.lastname: Li
authors.forename: Xiang-hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Ling-xiao
authors.initials: LX
authors.lastname: Xing
authors.forename: Xia
authors.initials: X
authors.lastname: Yan
authors.forename: Jun-ling
authors.initials: JL
authors.lastname: Wang
authors.forename: Feng-rong
authors.initials: FR
authors.lastname: Wang
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000328:Adult; D004305:Dose-Response Relationship, Drug; D004938:Esophageal Neoplasms / Q000139:chemically induced; D005434:Flow Cytometry; D005506:Food Contamination; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012306:Risk; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D012333:RNA, Messenger; C073119:TAP1 protein, human; D014255:Trichothecenes; C007262:deoxynivalenol