title: First systematic CGH-based analyses of ancient DNA samples of malformed fetuses preserved in the Meckel Anatomical Collection in Halle/Saale (Germany).
abstract: We present the first data on our comparative genomic hybridization (CGH)-based strategy for the analysis of ancient DNA (aDNA) samples extracted from fetuses preserved in the Meckel Anatomical Collection in Halle, Germany. The collection contains numerous differently fixed ancient samples of fetal malformations collected from the middle of the 18th to the early 19th century. The main objective of this study is to establish a "standard" aDNA extraction and amplification protocol as a prerequisite for successful CGH analyses to detect or exclude chromosomal imbalances possibly causative for the malformations described for the fetuses.
authors.affiliation: Institut für Humangenetik, Campus Virchow Klinikum, Charité, Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. holger.toennies@charite.de
authors.forename: H
authors.initials: H
authors.lastname: Tönnies
authors.forename: A
authors.initials: A
authors.lastname: Gerlach
authors.forename: R
authors.initials: R
authors.lastname: Klunker
authors.forename: R
authors.initials: R
authors.lastname: Schultka
authors.forename: L
authors.initials: L
authors.lastname: Göbbel
mesh_terms: D000013:Congenital Abnormalities / Q000235:genetics; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D005333:Fetus / Q000002:abnormalities*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009144:Museums; D009693:Nucleic Acid Hybridization; D013048:Specimen Handling
chemical_list: D004247:DNA