Prism-based excitation wavelength selection for multicolor fluorescence coincidence measurements.
We have designed and constructed a prism setup for multiple-color confocal fluorescence experiments. The prism setup permits easy selection of any color or any combination of colors from a multicolor light source, such as a mixed-gas argon-krypton-ion laser. The selected colors emerging from the prism setup are, by design, optimally overlapped in the focus of a high-numerical-aperture objective, such as that commonly used in single-molecule fluorescence experiments. The various excitation powers can be easily adjusted in this setup. We will exemplify the potential of this setup in single-molecule fluorescence emission spectroscopy.
Kassies
Roel
R
Institute for Biomedical Technology, Department of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands. r.kassies@utwente.nl
Lenferink
Aufried
A
Segers-Nolten
Ine
I
Otto
Cees
C