Rapid toluidine blue stain for Mohs' micrographic surgery.
BACKGROUND
Toluidine blue is a useful stain for detecting basal cell carcinoma during Mohs' micrographic surgery.


OBJECTIVE
To demonstrate the efficacy of alkalinization on the toluidine blue stain.


METHODS
A 1% aqueous toluidine blue-1% aqueous sodium borate solution was used to stain microscope slides for basal cell carcinoma during Mohs' micrographic surgery.


RESULTS
Total toluidine blue staining time was reduced to less than 2.5 minutes, without compromising the quality of the stain.


CONCLUSIONS
The rapid toluidine blue stain reduces staining time while maintaining staining quality, including the advantages specific to the toluidine blue stain.
Todd
Michael M
MM
Department of Dermatology, Geisinger Medical Center, Danville, Pennsylvania 17822, USA. mmtodd@geisinger.edu
Lee
James W
JW
Marks
Victor J
VJ
D002280:Basal Cell Carcinoma / Q000473:pathology / Q000601:surgery*; D004396:Coloring Agents; D006801:Humans; D015580:Mohs Surgery / Q000379:methods*; D012878:Skin Neoplasms / Q000473:pathology / Q000601:surgery*; D013194:Staining and Labeling / Q000379:methods*; D014048:Tolonium Chloride
D004396:Coloring Agents; D014048:Tolonium Chloride