title: Impact of MHC class II incompatibility on localization of mononuclear cell infiltrates to the bronchiolar compartment of orthotopic lung allografts.
abstract: Chronic pathological changes in transplanted lungs are unique because they center on the airways. We examined the relative role of MHC class I and II antigens in causing bronchial pathology in orthotopic lung transplants to rats maintained on cyclosporin A (CsA). Transplants mismatched for MHC class II antigens had significantly more peri-bronchiolar infiltrates than MHC class I incompatible transplants. No significant increase in infiltrates was found in lung transplants incompatible for MHC class I plus II antigens compared to MHC class II antigens alone. Immunohistochemistry demonstrated that MHC class II antigen expression was confined to macrophages in MHC class I incompatible transplants, but was upregulated on bronchial epithelium in transplants with MHC class II incompatibilities. Vascular endothelium was notably devoid of MHC class II antigen expression in all transplants. However, both peri-bronchial and peri-vascular infiltrates were frequently cuffed by alveolar macrophages and type II pneumocytes that expressed MHC class II antigens. PCR analysis demonstrated that IFN-gamma and regulated on activation, normal T cells expressed and secreted (RANTES) were upregulated in MHC class II incompatible transplants. Thus, MHC class II incompatible orthotopic lung transplants in rats maintained on CsA immunosuppression undergo a bronchiolcentric upregulation of alloantigens.
authors.affiliation: Transplantation Laboratory, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Shinji
authors.initials: S
authors.lastname: Nakashima
authors.forename: T Rinda
authors.initials: TR
authors.lastname: Soong
authors.forename: Karen
authors.initials: K
authors.lastname: Fox-Talbot
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Qian
authors.forename: Salma
authors.initials: S
authors.lastname: Rahimi
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Wasowska
authors.forename: Charles A
authors.initials: CA
authors.lastname: Rohde
authors.forename: Sabrina
authors.initials: S
authors.lastname: Chen
authors.forename: Joe G N
authors.initials: JG
authors.lastname: Garcia
authors.forename: William M
authors.initials: WM
authors.lastname: Baldwin
mesh_terms: D000818:Animals; D020296:Animals, Congenic; D001980:Bronchi / Q000276:immunology* / Q000473:pathology; D018946:Chemokine CCL5 / Q000378:metabolism; D005260:Female; D006648:Histocompatibility / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008168:Lung / Q000378:metabolism; D016040:Lung Transplantation; D008297:Male; D051381:Rats; D014184:Transplantation, Homologous
chemical_list: D018946:Chemokine CCL5; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma