title: Regeneration of rat auditory ossicles using recombinant human BMP-2/collagen composites.
abstract: Tympanoplasty operations to improve hearing impairment require a wide middle ear cavity and reconstruction of columellar formations. There is no specific material for use in the reconstruction of columellar formations. Tissue response to BMP has been employed as regenerative material. To our knowledge, however, there are no reports of the reconstruction of columellar formations using recombinant human bone morphogenetic protein-2/bovine collagen composites in the middle ear. The purpose of this study is to investigate whether recombinant human bone morphogenetic protein-2/bovine collagen composites (rhBMP-2 composites) are appropriate for use as regenerative material for tympanoplasty. In the form of pellets, rhBMP-2 composites were implanted as columellae into the tympanic cavity. At 2, 4, 6, 8, and 12 weeks after surgery, the middle ear of the animals (n = 4 at each week) was stained with hematoxylin-eosin for light microscopic observation. All composites were in the process of ossification or had ossified according to their developmental stages and were covered with a single layer of squamous or cuboidal epithelium. The new bone formed in these composites was persistently stable and displayed some columellar conditions assessed by histological examination. This study led to the conclusion that rhBMP-2 composites make excellent regenerative material for auditory ossicles.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine and Denistry, Okayama University, Okayama 700-8558, Japan. ytakeda@d1.dion.ne.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Takeda
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Tsujigiwa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagai
authors.forename: Junko
authors.initials: J
authors.lastname: Yoshinobu
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Okano
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Fukushima
authors.forename: Ayako
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshino
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nishizaki
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D001861:Bone Regeneration / Q000502:physiology*; D003094:Collagen; D004429:Ear Ossicles / Q000502:physiology*; D006801:Humans; D051381:Rats; D011994:Recombinant Proteins; D013997:Time Factors; D016212:Transforming Growth Factor beta; D014432:Tympanic Membrane
chemical_list: C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen