title: Expression of TARSH gene in MEFs senescence and its potential implication in human lung cancer.
abstract: To reveal the molecular mechanism of cellular senescence, we have surveyed the genes that are specifically upregulated via MEFs senescence by suppression subtractive hybridization method. We show here that mTARSH was induced particularly in the relative early phase of MEFs cellular senescence. Further structural analysis of mTARSH disclosed five splicing variants shared a common reading frame whose diversity was derived from the SH3-binding motif cluster in the middle of the gene. We also show that mTARSH mRNA predominantly expressed in lung and that conspicuous expression of TARSH was drastically declined in all several lung cancer cell lines we tested. Thus, TARSH presumably represents a trigger gene for evoking cellular senescence, which has also been suggested to be involved in the prevention of tumorigenesis.
authors.affiliation: Laboratory of Experimental Animal Model Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 36-3 Gengo, Morioka-Cho, Obu-city, Aichi 474-8522, Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Uekawa
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Terauchi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Nishikimi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Shimada
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Maruyama
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D051379:Mice
chemical_list: C439569:ABI3BP protein, human; D002352:Carrier Proteins; C527603:TARSH protein, mouse