Association of the HLA region with multiple sclerosis as confirmed by a genome screen using >10,000 SNPs on DNA chips.
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system, with a complex genetic background. Here, we present a genome screen for association in small scale, employing 11,555 single nucleotide polymorphisms (SNPs) on DNA chips for genotyping 100 MS patients stratified for HLA-DR2+ and 100 controls. More than 500 SNPs revealed significant differences between cases and controls before Bonferroni correction. A fraction of these SNPs was reanalysed in two additional cohorts of patients and controls, using high-throughput genotyping methods. A marker on chromosome 6p21.32 (rs2395182) yielded the highest significance level, validating the established HLA-DR association.
Gödde
René
R
Department of Human Genetics, Ruhr-University, 44780 Bochum, Germany. rene.goedde@rub.de
Rohde
Klaus
K
Becker
Christian
C
Toliat
Mahammad R
MR
Entz
Patricia
P
Suk
Anita
A
Müller
Norbert
N
Sindern
Eckhart
E
Haupts
Michael
M
Schimrigk
Sebastian
S
Nürnberg
Peter
P
Epplen
Jörg T
JT
D008040:Genetic Linkage; D005819:Genetic Markers; D005820:Genetic Testing; D016678:Genome; D006684:HLA-DR Antigens / Q000235:genetics*; D015802:HLA-DR2 Antigen / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D009103:Multiple Sclerosis / Q000209:etiology / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
D005819:Genetic Markers; D006684:HLA-DR Antigens; D015802:HLA-DR2 Antigen; D059811:HLA-DRB1 Chains