[Fluorescence characteristics of Tb3+ -pefeoxacin and determination of pefeoxacin].
Terbium (III) can react with Pefeoxacin (PEFX) to form 1:2 coordination compound in a near neutralmedium. Based on this reaction, we proposed a simple, rapid, and sensitive new method for the fluorimetric determination of Pefeoxacin and investigated the reaction conditions in detail; meanwhile we discussed the efficiency and mechanism of the intramolecular energy transfer. The results show that in the range of 0.01-1.67 microg x mL(-1), there is a linear relationship between the concentration of PEFX and its fluorescence intensity, and the regression equation is F = 120.01 C + 2.608, with the detection limit of 0.01 microg x mL(-1), the recovery within the range of 95.2%-101.7%, and the RSD of 2.9%-3.0%. This method has been successfully applied to the determination of Pefeoxacin in untreated human serum and gelatine capsule.
Zhou
Jing
J
Analytical and Testing Center, Shanxi Normal University, Linfen 041004, China.
Du
Li-Ming
LM
Chen
Cai-Ping
CP
D004735:Energy Transfer; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D057230:Limit of Detection; D008956:Models, Chemical; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013725:Terbium / Q000032:analysis / Q000737:chemistry*
D005456:Fluorescent Dyes; D013725:Terbium