title: Genetic and antigenic analyses of bovine respiratory syncytial virus detected in Japan.
abstract: Genetic and antigenic analyses of bovine respiratory syncytial virus were conducted on 12 field strains from Tohoku and Hokuriku districts in Japan during from 2002 to 2004. On the phylogenetic tree of the nucleotide sequences of the glycoprotein region, the examined strains fell in the same cluster as the strain isolated in Nebraska and were classified as the subgroup III. The examined strains were subdivided into 2 lineages (A, B). Isoleucine 200 of the epitope domain was replaced by threonine as a feature of the lineage B strains. The examined strains showed the nucleotide sequence homologies of 88.3-93.3% with the known Japanese strains classified as the subgroup II and of 86.1-96.6% with those in the subgroup III. No significant difference was found on the neutralization index between the examined strain and the 52-163-13 phylogenetically similar to the Japanese vaccine one. The results suggest that the subgroup III strains have existed in Japan and that epidemics of the strains could be protected due to the present vaccination.
authors.affiliation: Iwate Prefecture Central Livestock Hygiene Service Center, 390-5 Sunagome, Takizawa-mura.iwate 020-0173, Japan.
authors.forename: Gakuji
authors.initials: G
authors.lastname: Yaegashi
authors.forename: Yukio M
authors.initials: YM
authors.lastname: Seimiya
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Seki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D016000:Cluster Analysis; D000939:Epitopes / Q000378:metabolism; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D009500:Neutralization Tests / Q000662:veterinary; D010802:Phylogeny; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000939:Epitopes; D014760:Viral Fusion Proteins