[uPA expression of gastric cancer cell lines and its correlation with peritoneal seeding].
OBJECTIVE
To study the correlation between expression of urokinase-type plasminogen activator (uPA) and capability of tumor cell seeding to the peritoneal membrane by different gastric cancer lines.


METHODS
Expression of uPA in 4 human gastric cancer cell lines was examined by semi-quantitative RT-PCR, ELISA and Western blot. uPA activity was determined by an assay kit. After ip inoculation of cancer cells to nude mice, tumors on peritoneal membrane was grossly examined for tumor cell seedings.


RESULTS
SGC7901 was the highest in uPA expression among human gastric cancer cell lines AGS, SGC7901, MKN45, and MKN28. MKN45 had the strongest uPA activity, while AGS was lowest in both uPA expression and activity. Peritoneal seeding tumors of various sizes were observed in mice inoculated with SGC7901 and MKN45 cells. In addition to peritoneal seedings, bloody ascites was present in mice inoculated with MKN28. The MKN45-inoculated mice took the least time to develop tumors and had the shortest surviving period. No peritoneal seeding was seen in mice inoculated with AGS cells.


CONCLUSIONS
Three of 4 human gastric cancer cell lines studied express uPA mRNA and activity, which correlate with their peritoneal seeding potentials.
Ding
You-Cheng
YC
Department of General Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhu
Zheng-Gang
ZG
Liu
Bing-Ya
BY
Ji
Yu-Bao
YB
Chen
Xue-Hua
XH
Zhang
Yi
Y
Yu
Ying-Yan
YY
Lin
Yan-Zhen
YZ
D000230:Adenocarcinoma / Q000201:enzymology* / Q000556:secondary; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009366:Neoplasm Seeding; D010534:Peritoneal Neoplasms / Q000556:secondary*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator