Outer membrane protein (OMP) based vaccine for Neisseria meningitidis serogroup B.
A family of outer membrane lipoproteins of Neisseria meningitidis, LP2086, has been shown to induce serum bactericidal activity against a broad variety of meningococcal strains. Two sub-families of serologically distinct LP2086 proteins (A and B) have been identified. In the present study, we have shown that polyclonal anti-serum against rLP2086 is protective in vivo in an infant rat passive-protection model. Additionally, the LP2086 protein is displayed on the surface of 91% meningococcal strains as measured in a whole cell ELISA using polyclonal anti-sera raised against these proteins. We also demonstrate based on the reactivity of anti-rLP2086 antibody with recombinantly expressed C- and N-terminal fragments of rLP2086 in a Western blot assay that the C-terminal fragment of LP2086 dictates sub-family specificity and the N-terminal fragment determines the family specificity. A formulation containing family A and B of LP2086 potentially would provide broad protection against a majority of Neisseria meningitidis strains.
Pillai
Subramonia
S
Wyeth Vaccine Research, 401 North Middletown Road, Pearl River, NY 10965, USA. pillaisp@wyeth.com
Howell
Alan
A
Alexander
Kristin
K
Bentley
B Erin
BE
Jiang
Han-Qian
HQ
Ambrose
Karita
K
Zhu
Duzhang
D
Zlotnick
Gary
G
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D006801:Humans; D008589:Meningococcal Infections / Q000276:immunology / Q000517:prevention & control; D051379:Mice; D038541:Neisseria meningitidis, Serogroup B / Q000235:genetics / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D014614:Vaccines, Synthetic