[Molecular cloning and character analysis of the mouse zinc finger protein gene Zfp474 exclusively expressed in testis and ovary].
A novel mouse zinc finger protein gene that contains C2HC/C3H domain was first isolated by using a data-mining tool called Digital Differential Display (DDD) from the National Center for Biotechnology Information. The full-length cDNA of this transcript was deduced and further confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Total four exons of the mouse gene spaning a 29 869 bp genomic DNA sequence was mapped to chromosome 18D1. The cDNA encodes a novel protein of 347 amino acids and the protein contains four C2HC/C3H domains. Northern blot analyses revealed that Zfp474 mRNA was exclusively expressed in testis and ovary and had one transcript. We hypothesize that Zfp474 functions as a germ cell specific transcription factor that plays important roles in spermatid differentiation and oocyte development.
Zhou
Chang
C
Institude of Reproductive &Stem Cell Engineering, Central South University, Changsha 410078, China.
Li
Lu-Yun
LY
Lu
Guang-Xiu
GX
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D016335:Zinc Fingers / Q000502:physiology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D014157:Transcription Factors; C523348:Zfp474 protein, mouse