Stress-related Pseudomonas genes involved in production of bacteriocin LlpA.
Pseudomonas sp. BW11M1 produces a novel type of bacteriocin that inhibits the growth of Pseudomonas putida GR12-2R3 and some phytopathogenic fluorescent Pseudomonas. A collection of mutants was screened for altered bacteriocin production phenotypes. Strongly reduced bacteriocin production was found to be caused by inactivation of the recA gene or the spoT gene. Conversely, in a recJ mutant, the bacteriocin was constitutively overproduced. The same phenotype was observed for a mutant hit in a gene of unknown function. The predicted gene product belongs to a distinct subgroup of prokaryotic helicase-like proteins within the SWI/SNF family of regulatory proteins. One mutant that also exhibited a bacteriocin overproducer phenotype was deficient in the production of the peptidoglycan-associated lipoprotein OprL. This study shows that various environmental stress response pathways are involved in controlling expression of the Pseudomonas sp. BW11M1 bacteriocin.
de los Santos
Paulina Estrada
PE
Centre of Microbial and Plant Genetics, Faculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee Leuven, Belgium.
Parret
Annabel H A
AH
De Mot
René
R
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008074:Lipoproteins / Q000235:genetics; D009154:Mutation; D010457:Peptidoglycan; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001430:Bacteriocins; D029968:Escherichia coli Proteins; C031163:ExcC protein, E coli; D008074:Lipoproteins; D010457:Peptidoglycan