title: Fast determination of sugars in Coke and Diet Coke by miniaturized capillary electrophoresis with amperometric detection.
abstract: The fast separation capability of a novel miniaturized capillary electrophoresis with amperometric detection (CE-AD) system was demonstrated by determining sugar contents in Coke and diet Coke with an estimated separation efficiency of 60,000 TP/m. Factors influencing the separation and detection processes were examined and optimized. The end-capillary 300 microm Cu wire amperometric detector offers favorable signal-to-noise characteristics at a relatively low potential (+0.50 V vs. Ag/AgCl) for detecting sugars. Three sugars (sucrose, glucose, and fructose) have been separated within 330 s in a 8.5 cm length capillary at a separation voltage of 1000 V using a 50 mM NaOH running buffer (pH 12.7). Highly linear response is obtained for the above compounds over the range of 5.0 to 2.0 x 10(2) microg/mL with low detection limit, down to 0.8 microg/mL for glucose (S/N = 3). The injection-to-injection repeatability for analytes in peak current (RSD < 3.6%) and for migration times (RSD < 1.4%) was excellent. The new miniaturized CE-AD system should find a wide range of analytical applications involving assays of carbohydrates as an alternative to conventional CE and micro-CE.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
authors.forename: Qingcui
authors.initials: Q
authors.lastname: Chu
authors.forename: Liang
authors.initials: L
authors.lastname: Fu
authors.forename: Yueqing
authors.initials: Y
authors.lastname: Guan
authors.forename: Jiannong
authors.initials: J
authors.lastname: Ye
mesh_terms: D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002253:Carbonated Beverages / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008832:Microchemistry / Q000379:methods*; D008904:Miniaturization; D013997:Time Factors
chemical_list: D002241:Carbohydrates