[Direct determination of trace elements in human serum by electrothermal vaporization ICP-AES].
A method for direct determination of trace elements in human serum by electrothermal vaporization inductively coupled plasma atomic emission spectrometry (ETV-ICP-AES) with slurry sampling was developed. A polytetrafluoroethylene (PTFE) emulsion was used as a fluorinating agent to promote the vaporization of the analytes from graphite furnace, resulting in a remarkable improvement of analytical performances for the recommended method. The main factors affecting emission signals of the interested elements were investigated. The detection limits of 1.4 ng x mL(-1) for Cu and 2.9 ng x mL(-1) for Fe were achieved, and the precisions expressed as relative standard deviation (RSD) were less than 6.0% after optimization of the operating conditions.
Chen
Shi-zhong
SZ
Key Laboratory, Wuhan Polytechnic University, Wuhan 430023, China.
D001774:Blood Chemical Analysis; D004655:Emulsions; D006801:Humans; D057230:Limit of Detection; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014131:Trace Elements / Q000097:blood*; D014835:Volatilization
D004655:Emulsions; D014131:Trace Elements