[The effect of gemcitabine on proliferation and telomerase activity of Tca8113 cell lines].
OBJECTIVE
To investigate the effect of gemcitabine on the proliferation and telomerase activity of Tca8113 cell line cells.


METHODS
MTT assay, flow cytometry were used to examine the effect of gemcitabine on cell proliferation and cell cycle respectively, telomerase activity was detected with telomeric repeat amplification protocol (TRAP) and polyacrylamide gel electrophoresis (PAGE). The data were analyzed by independent samples t test and one-way ANOVA of SPSS10.0 for windows.


RESULTS
Cell proliferations were significantly inhibited in these cells after exposure to gemcitabine as well as cell cycles were arrested. Furthermore, the effect was closely associated with the concentration and time of gemcitabine. The average rate of cell proliferating inhibition in group 5.0 microg/ml at 72 h was 72.2%.The number of cells in S phase was decreased,the average rate of S phase in group 5.0 microg/ml at 72 h was 18.1%,which was lower than that in group 0 microg/ml(38.5%) (t=5.801,P<0.05).Longer exposure time and higher concentration to gemcitabine, much telomerase activity was inhibited. After 72h, the A values of group 0 and 5.0 microg/ml were 1.32+/-0.12, 0.28+/-0.02, respectively (F=811.208, P<0.05).


CONCLUSIONS
Gemcitabine could significantly inhibit Tca8113 proliferation and decrease its telomerase activity. Gemcitabine may be applied in clinical therapy of oral cancer.
Yao
Hua
H
Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University. Hangzhou 310003, Zhejiang Province, China. yaha@mail.hz.zj.cn
Wu
Qiu-Liang
QL
Wu
Bao-Xuan
BX
D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D019098:Telomerase / Q000378:metabolism*; D000093542:Gemcitabine
D003841:Deoxycytidine; D019098:Telomerase; D000093542:Gemcitabine