[Study on the resonance scattering light spectra of atrazine-RNA and its analytical application].
The Resonance Light Scattering (RLS) spectra of atrazine with yeast ribonucleic acid (yRNA) had been studied. At pH 1.50, the enhanced RLS of atrazine-yRNA system is characterized by the maximum scattering peaks at 320 nm and the RLS intensity is proportional to the concentration of yRNA. Under optimal conditions, the linear ranges of the calibration were 0.6-5.0 microg x mL(-1), the linear regression equation was I = 25.88 + 140.0 c(yRNA, microg x mL(-1)), r = 0.9975, with the limit of detection 20.7 ng x mL(-1) (3delta). The method was applied to the determination of yRNA in synthetic samples and RNA in salt cress (Thellungiella halophila) sample satisfactorily. Mechanism studies show that there are two interaction modes between atrazine and RNA: electrostatic mode and intercalative mode.
Ren
Li-ping
LP
College of Science, China Agricultural University, Beijing 100094, China.
Jiang
Shu-ren
SR
Rao
Zhen-hong
ZH
Xiong
Yan-mei
YM
Zhang
Chun-rong
CR
Wang
Dong-dong
DD
D001280:Atrazine / Q000737:chemistry* / Q000633:toxicity; D002138:Calibration; D005506:Food Contamination; D006540:Herbicides / Q000737:chemistry* / Q000633:toxicity; D008027:Light; D057230:Limit of Detection; D012313:RNA / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012542:Scattering, Radiation; D013057:Spectrum Analysis; D014732:Vibration
D006540:Herbicides; D012313:RNA; D001280:Atrazine