Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.
Qiu
Maofeng
M
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, People's Republic of China.
Wang
Jin
J
Wang
Hongxia
H
Chen
Zeliang
Z
Dai
Erhei
E
Guo
Zhaobiao
Z
Wang
Xiaoyi
X
Pang
Xin
X
Fan
Baoxing
B
Wen
Jie
J
Wang
Jian
J
Yang
Ruifu
R
D000914:Antibodies, Viral / Q000032:analysis; D001665:Binding Sites; D017934:Coronavirus / Q000737:chemistry / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D006801:Humans; D019251:Nucleocapsid / Q000032:analysis*; D012128:Respiratory Distress Syndrome / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
D000914:Antibodies, Viral