title: Detection and isolation of winter dysentery bovine coronavirus circulated in Korea during 2002-2004.
abstract: Although winter dysentery (WD) has been suspected to occur frequently in Korea, to date the exact epidemiology of WD has remained unknown. Therefore, we investigated the causative agents of WD by using electron microscopy, ELISA, RT-PCR, and nested PCR from 97 fecal samples of 32 WD-affected herds collected from 8 provinces during 2002-2004. The bovine coronavirus (BoCV) was consistently detected in all herds with WD. Of other pathogens, only coccidian oocyts were inconsistently but concurrently detected with BoCV. Ten isolates were identified as BoCV by immune electron microscope, immunofluorescent test and ELISA with antiserum to BoCV, and RT-PCR. From these results, it is concluded that WD caused by BoCV occurred in relatively high frequency and was widespread in Korea. The results provide important epidemiological data for the control and establishment of a surveillance system for WD in Korea.
authors.affiliation: Veterinary Medical Research Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Jeong
authors.forename: Gye-Yeop
authors.initials: GY
authors.lastname: Kim
authors.forename: Soon-Seek
authors.initials: SS
authors.lastname: Yoon
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: You-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Sung
authors.forename: Oh-Jun
authors.initials: OJ
authors.lastname: Jang
authors.forename: Sung-Shik
authors.initials: SS
authors.lastname: Shin
authors.forename: Hong-Bum
authors.initials: HB
authors.lastname: Koh
authors.forename: Bong-Joo
authors.initials: BJ
authors.lastname: Lee
authors.forename: Chai-Yong
authors.initials: CY
authors.lastname: Lee
authors.forename: Mun-Il
authors.initials: MI
authors.lastname: Kang
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Nam-Yong
authors.initials: NY
authors.lastname: Park
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary*; D017938:Coronavirus, Bovine / Q000302:isolation & purification* / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007723:Korea / Q000453:epidemiology; D008854:Microscopy, Electron / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary