title: Characterization of presenilin-amyloid precursor interaction using bacterial expression and two-hybrid systems for human membrane proteins.
abstract: An Escherichia coli system was used to produce the human membrane proteins presenilin 1 and amyloid precursor protein and to analyse their interaction. Our data indicate that the main binding site for amyloid precursor protein is located in the N-terminal three-transmembrane segments of presenilin and not in the proposed active site containing the two conserved aspartate residues. The data also suggest the presence of an additional segment of sufficient hydrophobicity at the C-terminus of PS1 to act potentially as a transmembrane segment. The implications of these findings for the function of gamma-secretase are discussed.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3US, UK.
authors.forename: Mona
authors.initials: M
authors.lastname: Harnasch
authors.forename: Sandra
authors.initials: S
authors.lastname: Grau
authors.forename: Christian
authors.initials: C
authors.lastname: Behrends
authors.forename: Simon L
authors.initials: SL
authors.lastname: Dove
authors.forename: Ann
authors.initials: A
authors.lastname: Hochschild
authors.forename: Maria-Karnina
authors.initials: MK
authors.lastname: Iskandar
authors.forename: Weiming
authors.initials: W
authors.lastname: Xia
authors.forename: Michael
authors.initials: M
authors.lastname: Ehrmann
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D053764:Presenilin-1; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D011994:Recombinant Proteins