title: Maintenance of spermatogenesis requires TAF4b, a gonad-specific subunit of TFIID.
abstract: The establishment and maintenance of spermatogenesis in mammals requires specialized networks of gene expression programs in the testis. The gonad-specific TAF4b component of TFIID (formerly TAF(II)105) is a transcriptional regulator enriched in the mouse testis. Herein we show that TAF4b is required for maintenance of spermatogenesis in the mouse. While young Taf4b-null males are initially fertile, Taf4b-null males become infertile by 3 mo of age and eventually exhibit seminiferous tubules devoid of germ cells. At birth, testes of Taf4b-null males appear histologically normal; however, at post-natal day 3 gonocyte proliferation is impaired and expression of spermatogonial stem cell markers c-Ret, Plzf, and Stra8 is reduced. Together, these data indicate that TAF4b is required for the precise expression of gene products essential for germ cell proliferation and suggest that TAF4b may be required for the regulation of spermatogonial stem cell specification and proliferation that is obligatory for normal spermatogenic maintenance in the adult.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Allison E
authors.initials: AE
authors.lastname: Falender
authors.forename: Richard N
authors.initials: RN
authors.lastname: Freiman
authors.forename: Kenneth G
authors.initials: KG
authors.lastname: Geles
authors.forename: Kirk C
authors.initials: KC
authors.lastname: Lo
authors.forename: KeumSil
authors.initials: K
authors.lastname: Hwang
authors.forename: Dolores J
authors.initials: DJ
authors.lastname: Lamb
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Morris
authors.forename: Robert
authors.initials: R
authors.lastname: Tjian
authors.forename: JoAnne S
authors.initials: JS
authors.lastname: Richards
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D002455:Cell Division / Q000502:physiology; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D051379:Mice; D013087:Spermatids / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D035381:TATA-Binding Protein Associated Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D035381:TATA-Binding Protein Associated Factors; C491103:Taf4b protein, mouse; D035362:Transcription Factor TFIID