title: Protective effects of Rheum tanguticum polysaccharide against hydrogen peroxide-induced intestinal epithelial cell injury.
abstract: OBJECTIVE
  To describe the effect of Rheum tanguticum polysaccharide (RTP) on hydrogen peroxide-induced human intestinal epithelial cell injury.
  
  
  METHODS
  Hydrogen peroxide (100 micromol/L) was introduced to induce human intestinal epithelial cell injury. Cells were pretreated with RTP (30,100,300 microg/mL) for 24 h before exposure to hydrogen peroxide. Cell viability was detected by MTT assay and morphological observation. Acridine orange staining and flow cytometry were performed to assess cell apoptosis. Lactate dehydrogenase (LDH) activity, production of malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured by spectrophotometry with corresponding assay kits.
  
  
  RESULTS
  Following exposure to H(2)O(2), a marked decrease in cell survival and SOD activity, increased production of MDA, LDH leakage and cell apoptosis were found. Pretreatment of the cells with RTP could significantly elevate cell survival, SOD activity and decrease the level of MDA, LDH activity and cell apoptosis.
  
  
  CONCLUSIONS
  RTP may have cytoprotective and anti-oxidant effects against H(2)O(2)-induced intestinal epithelial cell injury by inhibiting cell apoptosis and necrosis. This might be one of the possible mechanisms of RTP for the treatment of ulcerative colitis in rats.
authors.affiliation: Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: Lin-Na
authors.initials: LN
authors.lastname: Liu
authors.forename: Qi-Bing
authors.initials: QB
authors.lastname: Mei
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Liu
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Wang
authors.forename: Ru-Tao
authors.initials: RT
authors.lastname: Wang
mesh_terms: D002460:Cell Line; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D012250:Rheum / Q000737:chemistry*
chemical_list: D016877:Oxidants; D010936:Plant Extracts; D011134:Polysaccharides; D020011:Protective Agents; D006861:Hydrogen Peroxide