CODEHOP-mediated PCR - a powerful technique for the identification and characterization of viral genomes.
Consensus-Degenerate Hybrid Oligonucleotide Primer (CODEHOP) PCR primers derived from amino acid sequence motifs which are highly conserved between members of a protein family have proven to be highly effective in the identification and characterization of distantly related family members. Here, the use of the CODEHOP strategy to identify novel viruses and obtain sequence information for phylogenetic characterization, gene structure determination and genome analysis is reviewed. While this review describes techniques for the identification of members of the herpesvirus family of DNA viruses, the same methodology and approach is applicable to other virus families.
Rose
Timothy M
TM
Department of Pathobiology, Box 357238, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA. trose@u.washington.edu
D020816:Amino Acid Motifs; D005075:Biological Evolution; D016679:Genome, Viral; D006564:Herpesviridae / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics*
D014764:Viral Proteins