Formation of compound I by photo-oxidation of compound II.
[reaction: see text] Compound I is the heme-iron(IV)-oxo porphyrin radical cation formed in peroxidase and catalase enzymes by reaction with hydrogen peroxide. As an alternative to chemical oxidations of porphyrin-iron(III) species, various compound I species were produced by 355 nm laser flash photolysis photooxidation of the corresponding compound II species, porphyrin-iron(IV)-oxo derivatives. The method is demonstrated by production and kinetic studies of the compound I species from 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin-iron, from horseradish peroxidase, and from wild-type horse skeletal myoglobin.
Zhang
Rui
R
Department of Chemistry, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607, USA.
Chandrasena
R Esala P
RE
Martinez
Enrique
E
Horner
John H
JH
Newcomb
Martin
M
D000818:Animals; D006735:Horseradish Peroxidase / Q000378:metabolism; D006736:Horses; D007501:Iron / Q000737:chemistry*; D007697:Kinesics; D008665:Metalloporphyrins / Q000138:chemical synthesis* / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry; D010084:Oxidation-Reduction; D010777:Photochemistry / Q000379:methods
D008665:Metalloporphyrins; D009211:Myoglobin; C066317:ferryl iron; D007501:Iron; D006735:Horseradish Peroxidase