Dengue virus detection by using reverse transcription-polymerase chain reaction in saliva and progeny of experimentally infected Aedes albopictus from Brazil.
Oral susceptibility and vertical transmission of dengue virus type 2 (DENV-2) in an Aedes albopictus sample from Rio de Janeiro was estimated. The infection (36.7%) and transmission (83.3%) rates for Ae. albopictus were higher than those of an Ae. aegypti colony used as control, 32.8 and 60%, respectively. Fourth instar larvae and females descendants of 48.5 and 39.1% of experimentally infected Ae. albopictus showed to harbor the virus. The oral susceptibility and the high capacity to assure vertical transmission exhibited by Ae. albopictus from Brazil reinforce that this species may play a role in the maintenance of the virus in nature and be a threat for dengue control in the country.
Castro
Márcia Gonçalves de
MG
Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. mcastro@ioc.fiocruz.br
Nogueira
Rita Maria Ribeiro
RM
Schatzmayr
Hermann Gonçalves
HG
Miagostovich
Marize Pereira
MP
Lourenço-de-Oliveira
Ricardo
R
D000330:Aedes / Q000235:genetics / Q000821:virology*; D000818:Animals; D001938:Brazil; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007303:Insect Vectors / Q000235:genetics / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva / Q000821:virology*; D013045:Species Specificity
D012367:RNA, Viral