title: Evaluation of a soybean lectin-based method for the enrichment of erythroblasts.
abstract: We performed a comparative study of the enrichment of erythroblasts by a soybean agglutinin galactose-specific lectin method and a standardized magnetic cell-sorting (MACS) protocol. Blood samples, obtained from 11 pregnant women at between 11 and 40 weeks of gestation, were split and examined by each method in parallel. The number of erythroblasts recovered by the lectin method was approximately eightfold higher than the number obtained by MACS. Our data suggest that the lectin-based method may provide a better approach for the enrichment of rare fetal erythroblasts from maternal blood.
authors.affiliation: Laboratory for Prenatal Medicine, University Women's Hospital/Department of Research, Spitalstr. 21, CH 4031 Basel, Switzerland.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Babochkina
authors.forename: Susanne
authors.initials: S
authors.lastname: Mergenthaler
authors.forename: Olaf
authors.initials: O
authors.lastname: Lapaire
authors.forename: Vivian
authors.initials: V
authors.lastname: Kiefer
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yura
authors.forename: Kimie
authors.initials: K
authors.lastname: Koike
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Holzgreve
authors.forename: Sinuhe
authors.initials: S
authors.lastname: Hahn
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004900:Erythroblasts / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D018189:Immunomagnetic Separation; D007202:Indicators and Reagents; D037121:Plant Lectins; D011247:Pregnancy; D030262:Soybean Proteins; D013025:Glycine max; D010835:Phytohemagglutinins
chemical_list: D007202:Indicators and Reagents; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins