[The study of absorption spectrum for cell substrate].
The authors collected the absorption spectrum of RPMI 1640 and DMEM substrates that cultivated Hela and CNE by UV-3101 spectrophotometer and analysed the absorbability of proteins in the substrate. The absorption peaks of the RPMI 1 640 culture medium that cultivated cells for different times shifted from 227 to 222 or 218 nm and from 278 to 280 nm respectively; while during growing course of cultivated cells, one of the absorption peaks of DMEM culture medium shifted from 224 nm to one near 221 nm, and the absorption peak 278 nm almost had no shift. All of these shifts show that the content of each amino acid such as tryptophan and casein has already changed. That is, during the growing course of cultivating cancer cells, the tryptophan and casein were not depleted equivalently. In the growth period of Hela and CNE, they consumed different amino acid. So they need different component proportion for amino acid.
Zhao
Yuan-Li
YL
Anhui Institute of Optics and Mechanics, Chinese Academy of Sciences, Hefei 230031, China.
Zhang
Feng-Qiu
FQ
Ge
Xiang-Hong
XH
Yao
Shu-Xia
SX
Liang
Er-jun
EJ
D000042:Absorption; D000818:Animals; D045744:Cell Line, Tumor / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D022082:Cellular Structures; D006801:Humans; D008027:Light; D018625:Microscopy, Atomic Force / Q000379:methods; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods / Q000706:statistics & numerical data; D020349:Surface Plasmon Resonance; D014466:Ultraviolet Rays / Q000009:adverse effects