title: Effects of Vitamin E on airway responses and biochemical parameters in guinea pigs sensitized to ovalbumin.
abstract: The effect of dietary supplementation with Vitamin E was studied in sensitized guinea pigs. After measurement of baseline airway reactivity and sensitization with ovalbumin, the animals were randomized into two groups: Group A, on a commercial feed and Group B, on dietary supplementation with oral Vitamin E (0.7 IU/kg). These were challenged with inhaled ovalbumin after 4 weeks. The following outcomes were studied: airway responses to ovalbumin inhalation, airway reactivity, sodium and calcium ion influx in isolated tracheal cells, Na+ K+ ATPase and Ca2+ ATPase activity in tracheal homogenate and plasma malonaldehyde. Sensitization increased airway reactivity in Group A but not in Group B. The tracheal cells of animals in Group B showed significantly lower rates of 45Ca and 22Na influx and lower activities of tracheal Na+ K+ ATPase and Ca2+ ATPase as compared to Group A. Plasma malonaldehyde was similar between two groups. We concluded that Vitamin E suppresses the increase in airway reactivity following sensitization and has membrane stabilizing actions.
authors.affiliation: Department of Medicine, Pulmonary Allergy and Critical Care Division, University Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Deepika
authors.initials: D
authors.lastname: Jain
authors.forename: S K
authors.initials: SK
authors.lastname: Chhabra
authors.forename: H G
authors.initials: HG
authors.lastname: Raj
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004035:Diet Therapy / Q000379:methods; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D007554:Isotopes / Q000493:pharmacokinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010047:Ovalbumin; D011897:Random Allocation; D013997:Time Factors; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D007554:Isotopes; D014810:Vitamin E; D006632:Histamine; D010047:Ovalbumin; D000251:Adenosine Triphosphatases; D002118:Calcium