title: Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach.
abstract: Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.
authors.affiliation: Marine Environmental Quality Section, Institute of Ocean Sciences, Fisheries and Oceans Canada, P.O. Box 6000, Sidney, BC, Canada V8L 4B2.
authors.forename: Kelsey A
authors.initials: KA
authors.lastname: Miller
authors.forename: Marta G L
authors.initials: MG
authors.lastname: Assunção
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Dangerfield
authors.forename: Stelvio M
authors.initials: SM
authors.lastname: Bandiera
authors.forename: Peter S
authors.initials: PS
authors.lastname: Ross
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods; D001955:British Columbia; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D015151:Immunoblotting; D008099:Liver / Q000473:pathology; D046049:Phoca / Q000378:metabolism*; D012867:Skin / Q000473:pathology; D019324:beta-Naphthoflavone
chemical_list: D004785:Environmental Pollutants; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1