Shiquandabutangjiaweibang inhibits tumor metastasis and angiogenesis via regulation of topoisomerase-1.
Shiquandabutangjiaweibang (SDJ) is a traditional medicine prescription used for increasing body resistance against cancer. In the present study, the effect of SDJ extract on tumor metastasis and angiogenesis was evaluated. SDJ showed cytotoxicity against P388 (leukemia cells) and B16-F10 (murine melanoma cells) to 60% of control at 1 mg. SDJ significantly inhibited lung metastasis and also restored the number of platelets in C57BL/6 mice with thrombocytopenia induced by intravenous injection of B16-F10 cells. SDJ significantly disrupted chick embryonic angiogenesis in the chorioallantoic membrane (CAM). Interestingly, SDJ suppressed DNA topoisomerase I in a concentration-dependent manner. These results suggest that SDJ can be a potent inhibitor of metastasis and angiogenesis, at least in part, via regulation of topoisomerase I.
Kim
Sung Hoon
SH
Department of Oncology, Graduate School of East-West Medical Science, Kyung Hee University, 1 Seochunri, Yongin 449-701, South Korea. sungkim7@khu.ac.kr
Lee
Tae-Hyung
TH
Yang
Deok-Chun
DC
Kim
Hyung-Min
HM
Kim
Jeung-Beum
JB
Park
Mi-Kyung
MK
Bae
Yong-Soo
YS
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D049033:Chorioallantoic Membrane / Q000187:drug effects; D004264:DNA Topoisomerases, Type I / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D007941:Leukemia P388 / Q000473:pathology / Q000517:prevention & control; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008297:Male; D008518:Medicine, East Asian Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009368:Neoplasm Transplantation / Q000379:methods; D009374:Neoplasms, Experimental / Q000188:drug therapy; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D028321:Plant Preparations / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D013194:Staining and Labeling; D013921:Thrombocytopenia / Q000139:chemically induced; D013922:Thrombocytosis / Q000139:chemically induced; D014407:Tumor Cells, Cultured
D028321:Plant Preparations; C506674:shiquandabutangjiaweibang; D004264:DNA Topoisomerases, Type I