[Expression of imprinted gene Grb10 in human oocytes and preimplantation embryos].
OBJECTIVE
To investigate the mRNA expression of imprinted gene Grb10, a candidate gene for Silver-Russell syndrome (SRS), in human oocytes and preimplantation embryos for understanding the role of Grb10 in embryogenesis and assisted reproductive technology (ART).


METHODS
Nested reverse transcriptase (RT)-PCR was used to analyze Grb10 gene expression in human oocytes and preimplantation embryos.


RESULTS
The transcripts of Grb10 were detected in human oocytes and preimplantation embryos at all stages except for the 2-cell stage.


CONCLUSIONS
Grb10 gene is expressed in preimplantation embryos with close association with embryogenesis.
Shen
Wen-Jie
WJ
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. swjywf@yahoo.com.cn
Kong
Ling-Hong
LH
Chen
Shi-Ling
SL
Li
Hong
H
Xing
Fu-Qi
FQ
D001755:Blastocyst / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D051383:GRB10 Adaptor Protein / Q000096:biosynthesis* / Q000235:genetics; D018392:Genomic Imprinting; D006801:Humans; D009865:Oocytes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051383:GRB10 Adaptor Protein