Detection and location of Helicobacter pylori in human gastric carcinomas.
OBJECTIVE
To define the infection status of Helicobacter pylori in 109 patients with gastric cancers and H pylori localization in gastric carcinoma tissues in South China.


METHODS
The incidence of H pylori infection in gastric carcinomas was estimated by polymerase chain reaction (PCR), simultaneously; both morphological features and the localization of H pylori in gastric carcinomas were demonstrated by Warthin-Starry (WS) staining. The relationships between H pylori infection and the clinical-pathologic factors of gastric carcinomas were analyzed by software SPSS10.0.


RESULTS
H pylori was found in 42 (39.03%) and 58 (53.21%) cases of 109 patients with gastric carcinomas by PCR and WS, respectively. H pylori infection rate detected in gastric carcinomas by WS was higher than that by PCR (chi2 = 9.735, P < 0.005 < 0.01). WS stain showed that H pylori existed in the gastric antrum mucus, mucosal gland of normal tissues adjacent to gastric carcinomas and the gland, mucus pool of cancer tissues. The positive rate of H pylori in normal tissues adjacent to carcinomas was higher than that in cancer tissues (chi2 = 15.750, P < 0.005 < 0.01). No significant differences in age, sex, site, histological types and lymph node metastasis were found between H pylori-positive gastric carcinomas and H pylori-negative cases by both methods, but there were statistically significant differences of H pylori positive rate between early and advanced stage of gastric carcinomas (chi2 = 4.548 or 5.922, P = 0.033 or 0.015 < 0.05).


CONCLUSIONS
These results suggested that H pylori infection might play a certain role in the early stage of carcinogenesis of human gastric mucosa epithelia.
Tang
Yun-Lian
YL
Department of Pathology, Medical School, Nanhua University, Hengyang 421001, Hunan Province, China.
Gan
Run-Liang
RL
Dong
Bi-Hua
BH
Jiang
Ri-Chen
RC
Tang
Rong-Jun
RJ
D000230:Adenocarcinoma / Q000453:epidemiology* / Q000473:pathology; D018279:Carcinoma, Signet Ring Cell / Q000453:epidemiology / Q000473:pathology; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000453:epidemiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000473:pathology
D004269:DNA, Bacterial