The fibroblast response to tubes exhibiting internal nanotopography.
The use of three-dimensional scaffolds in cell and tissue engineering is widespread; however, the use of such scaffolds, which bear additional cellular cues such as nanotopography, is as yet in its infancy. This paper details the novel fabrication of nylon tubes bearing nanotopography via polymer demixing, and reports that the topography greatly influenced fibroblast adhesion, spreading, morphology and cytoskeletal organisation. The use of such frameworks that convey both the correct mechanical support for tissue formation and stimulate cells through topographical cues may pave the way for future production of intelligent materials and scaffolds.
Berry
Catherine Cecilia
CC
Centre for Cell Engineering, University of Glasgow, IBLS, Joseph Black Building University, Glasgow G12 8QQ, UK. catherine.berry@bio.gla.ac.uk
Dalby
Matthew J
MJ
McCloy
David
D
Affrossman
Stanley
S
D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D048429:Cell Size; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D009757:Nylons / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D009757:Nylons