Strategies for the study of meiosis in rye.
We describe how we are furthering our understanding of meiosis in rye (Secale cereale L.) using a combination of cytogenetic and molecular biological approaches. Fluorescent in situ hybridisation, electron microscopy of synaptonemal complexes, sequencing of meiosis-specific genes, and the immunolocalisation of recombinogenic proteins are being combined to build up phenotypic "identikits" of wild type, asynaptic mutants sy1 and sy9, and desynaptic mutant sy10. From this information, we review the status of our current understanding of the genetic control of meiosis in rye, and consider strategies for determining more precisely the interrelationships between meiosis-specific genes and their products.
Jenkins
G
G
Institute of Biological Sciences, University of Wales Aberystwyth, Aberystwyth, UK. gmj@aber.ac.uk
Mikhailova
E I
EI
Langdon
T
T
Tikholiz
O A
OA
Sosnikhina
S P
SP
Jones
R N
RN
D018744:DNA, Plant / Q000235:genetics; D005456:Fluorescent Dyes; D017343:Genes, Plant; D006580:Genetic Carrier Screening; D008540:Meiosis / Q000235:genetics*; D018613:Microscopy, Confocal; D010940:Plant Proteins / Q000235:genetics*; D012434:Secale / Q000166:cytology / Q000235:genetics* / Q000648:ultrastructure; D014908:Triticum / Q000235:genetics
D018744:DNA, Plant; D005456:Fluorescent Dyes; D010940:Plant Proteins