Bovine BMP osteoinductive potential enhanced by functionalized dextran-derived hydrogels.
This study evaluated functionalized dextran-derived hydrogels as BMP carriers using both in vitro and in vivo models. In vitro release kinetics indicated that dextran-derived hydrogels could retain rhBMP-2 growth factor in a variable manner depending on their functionalization ratio. The potential of these hydrogels when combined with extracted bovine BMP to enhance the bone formation was evaluated in a rat ectopic model. The largest osteoinduction was found when using hydrogels exhibiting the highest growth factor retention capacity. In addition, some implanted hydrogels demonstrated a capacity to induce an in-vivo calcification certainly related to their chemical composition. These properties make these materials interesting osteoconductive BMP carriers, allowing to decrease the amount of implanted factor required for bone regeneration.
Maire
Marion
M
Laboratoire de Recherches Orthopédiques, UMR 7052 CNRS-SPI, Faculté de Médecine Lariboisière-Saint-Louis, Université Paris 7, 10 Avenue de Verdun, 75010 Paris, France.
Chaubet
Frederic
F
Mary
Pierrer
P
Blanchat
Cinderella
C
Meunier
Alain
A
Logeart-Avramoglou
Delphine
D
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage* / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D004343:Drug Implants / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000737:chemistry
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D003911:Dextrans; D004343:Drug Implants; D020100:Hydrogels; D016212:Transforming Growth Factor beta