title: [The interaction of berberine and human serum albumin].
abstract: The interaction of berberine and Human Serum Albumin (HSA) has been studied by fluorescence and UV-Vis absorption spectroscopy. With fluorescence quenching method, the binding constant K was found to be 1.168 x 10(5) L x mol(-1) and the number of binding site n was 5.26. The binding distance (R = 3.44 nm) and energy transfer efficiency (E = 0.303) were also obtained according to Förster's non-radiation energy transfer theory. The energy transfer path between tyrosine residual and tryptophan residual was cut by berberine in the site of HSA and weak fluorescence berberine-HSA complex was also observed.
authors.affiliation: Department of Chemistry, Xuzhou Normal University, Xuzhou 221009, China.
authors.forename: Chang-chun
authors.initials: CC
authors.lastname: Zhao
authors.forename: Wei-fa
authors.initials: WF
authors.lastname: Zheng
authors.forename: Meng-qiu
authors.initials: MQ
authors.lastname: Li
mesh_terms: D001599:Berberine / Q000493:pharmacokinetics* / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects*; D004347:Drug Interactions; D004735:Energy Transfer / Q000187:drug effects*; D005453:Fluorescence; D006801:Humans; D008956:Models, Chemical; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D001599:Berberine; D014443:Tyrosine; D014364:Tryptophan