Determination of beta-agonists in liver and retina by liquid chromatography-tandem mass spectrometry.
A liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the determination of bromobuterol, cimaterol, clenbuterol, clenpenterol, hydroxymethylclenbuterol, isoxsuprine, mabuterol, ractopamine, ritrodrine, salbutamol, terbutaline, and tulobuterol residues in bovine liver and retina is reported. This procedure uses enzymatic digestion, liquid-liquid extraction, and cleanup on Oasis HLB solid-phase extraction cartridges, followed by determination of the residues by LC-tandem quadrupole MS using atmospheric pressure chemical ionization in the positive ion mode. Overall average recoveries ranged from 23 to 76% for liver and 34 to 77% for retina. The mean values for samples fortified at levels between 0.5-2.0 microg/kg (liver) and 5-20 microg/kg (retina) agreed within 98-118% of the spiked levels, with coefficients of variation ranging from 6 to 20%. The decision limits, CCalpha, ranged from 0.1 to 0.3 microg/kg for liver, 1-3 microg/kg for retina, and detection capabilities, CCbeta, from 0.2-0.5 microg/kg for liver and 2-5 microg/kg for retina.
Fesser
Adrian C E
AC
Centre for Veterinary Drug Residues, Canadian Food Inspection Agency, Saskatoon Laboratory, 116 Veterinary Rd, Saskatoon, S7N 2R3, Canada. afesser@inspection.gc.ca
Dickson
Leslie C
LC
MacNeil
James D
JD
Patterson
John R
JR
Lee
Stephen
S
Gedir
Ronald
R
D000318:Adrenergic beta-Agonists / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D007477:Ions; D008099:Liver / Q000378:metabolism*; D008110:Liver Extracts / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D009584:Nitrogen / Q000737:chemistry; D011312:Pressure; D012160:Retina / Q000378:metabolism*; D013997:Time Factors; D014867:Water
D000318:Adrenergic beta-Agonists; D007477:Ions; D008110:Liver Extracts; D014867:Water; D009584:Nitrogen