title: Anticoagulant and antifibrinogenolytic properties of the aqueous extract from Bauhinia forficata against snake venoms.
abstract: The aqueous extract from aerial parts of Bauhinia forficata was able to neutralize the clotting activity induced by Bothrops and Crotalus crude venoms. The clotting time, upon human plasma, induced by B. moojeni venom was significantly prolonged. Clotting and fibrinogenolytic activities induced by isolated thrombin-like enzyme from Bothrops jararacussu were totally inhibited after incubation at different ratios. The extract was not able to neutralize the hemorrhagic activity induced by an Bothrops venoms, but it efficiently inhibited the edema induced by Crotalus durissus terrificus venom and isolated PLA2s. In addition, it did not inhibited the phospholipase A2 activity of Bothrops snake venoms. Interaction studies between Bauhinia forficata extract and snake venoms, when analyzed by SDS-PAGE, did not reveal any apparent degradation of the venom proteins. This extract is a promising source of natural inhibitors of serine-proteases involved in blood clotting disturbances induced by snake venoms.
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP, 14096-900 Ribeirão Preto, SP, Brazil.
authors.forename: Clayton Z
authors.initials: CZ
authors.lastname: Oliveira
authors.forename: Victor A
authors.initials: VA
authors.lastname: Maiorano
authors.forename: Silvana
authors.initials: S
authors.lastname: Marcussi
authors.forename: Carolina D
authors.initials: CD
authors.lastname: Sant'ana
authors.forename: Ana H
authors.initials: AH
authors.lastname: Januário
authors.forename: Miriam V
authors.initials: MV
authors.lastname: Lourenço
authors.forename: Suely V
authors.initials: SV
authors.lastname: Sampaio
authors.forename: Suzelei C
authors.initials: SC
authors.lastname: França
authors.forename: Paulo S
authors.initials: PS
authors.lastname: Pereira
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D000933:Antifibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D036481:Bauhinia / Q000737:chemistry / Q000378:metabolism*; D003435:Crotalid Venoms / Q000009:adverse effects / Q000037:antagonists & inhibitors; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012910:Snake Venoms / Q000009:adverse effects / Q000037:antagonists & inhibitors* / Q000737:chemistry; D014867:Water / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000925:Anticoagulants; D000933:Antifibrinolytic Agents; D003435:Crotalid Venoms; D010936:Plant Extracts; D012910:Snake Venoms; D014867:Water; C030961:botrocetin