title: [High cell density fed-batch culture of E.coli expressing rhVEGF121].
abstract: OBJECTIVE
  To investigate the optimal high cell density fermentation conditions of recombinant E.coli BL21/pET- 24a/hVEGF(121) expressing recombinant human vascular endothelial growth factor (rhVEGF(121)).
  
  
  METHODS
  The effects of the composition of the fermentation medium, induction time and fed-batch carbon sources on the expression level of rhVEGF(121) and cell output were analyzed.
  
  
  CONCLUSIONS
  When cultured in modified M9 medium and induced for 4 h in the presence of 0.5 mmol/L IPTG at 37 degrees celsius; with glycerol as the carbon sources by continuous fed-batch mode, the recombinant E.coli expressed rhVEGF(121) at the level up to 23% of the total proteins and the yield reached 68 g/L. The optimized fermentation condition for recombinant E.coli enables high expression level of rhVEGF(121).
authors.affiliation: Institute of Molecular Immunology, Southern Medical University, Guangzhou 510515, China. hzm@fimmu.com
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Ming-Qian
authors.initials: MQ
authors.lastname: Zhou
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Meng
authors.forename: Jie-Zuan
authors.initials: JZ
authors.lastname: Yang
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A