title: Mechanical and electrophysiological effects of isoprostanes on bronchial arterial smooth muscle.
abstract: We investigated the mechanical and electrophysiological responses of human and porcine bronchial arterial smooth muscle to isoprostanes (metabolites of membrane lipid peroxidation). These evoked a constrictor response which was sensitive to blockade of thromboxane receptors, as well as to a non-specific tyrosine kinase inhibitor and an inhibitor of Rho-kinase. The patch clamp technique was used to characterize the K+ and Ca2+ currents in these tissues, and to show that isoprostanes caused a brief enhancement of K+ currents followed by prolonged and marked suppression of the same.
authors.affiliation: Father Sean O'Sullivan Research Centre, Firestone Institute for Respiratory Health, St. Joseph's Hospital, Department of Medicine, McMaster University, Hamilton, Ontario, Canada. janssenl@mcmaster.ca
authors.forename: L J
authors.initials: LJ
authors.lastname: Janssen
authors.forename: T
authors.initials: T
authors.lastname: Tazzeo
authors.forename: J
authors.initials: J
authors.lastname: Miller
mesh_terms: D000818:Animals; D001981:Bronchial Arteries / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D004594:Electrophysiology; D006801:Humans; D028421:Isoprostanes / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D013552:Swine
chemical_list: D028421:Isoprostanes; D011188:Potassium; D002118:Calcium