Weighting by heritability for detection of quantitative trait loci with microarray estimates of gene expression.
Heritable differences in transcribed RNA levels can be mapped as quantitative trait loci (QTLs). Transcribed RNA levels are often measured by hybridization to microarrays of oligonucleotide probes, in which each transcript is represented by multiple probes. The use of recombinant inbred lines allows an estimate of the heritability of expression measured by individual probes. This heritability varies greatly. We have tested heritability-weighted averages to define expression of a transcript and found that these allow detection of more QTLs than previously described methods.
Manly
Kenneth F
KF
Department of Pathology, University of Tennessee Health Science Center, 855 Monroe Avenue, Memphis, TN 38163, USA. kmanly@tennessee.edu
Wang
Jintao
J
Williams
Robert W
RW
D000818:Animals; D001921:Brain / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D040641:Quantitative Trait Loci / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger