title: [TLC-FT-SERS study on a pair of optic isomers in ephedra].
abstract: A new method for analyzing the ingredients of a pair of optic isomers in ephedra, nor-ephedrine and nor-pseudo-ephedrine, using hyphenated high-efficiency thin layer chromatography (TLC) and surface-enhanced Raman spectroscopy (SERS) techniques, is reported. The results show that the characteristic spectral bands of nor-ephedrine and nor-pseudo-ephedrine can be obtained from the TLC spot with 8 microg sample of about 2.0 mm in diameter. The difference between the SERS and solid spectra was found. Spectral bands at 1004 cm(-1) and 1605 cm(-1) were found greatly enhanced. Molecule was absorbed in surface silver sol by pi electrons in ring. Under similar experimental conditions the spectral information of Levo-nor-ephedrine ramifications TLC-SERS is rich with strong credibility, whereas dextral-nor-ephedrine ramifications show a relatively strong fluorescence backdrop with less spectral information and weak credibility. The effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100037, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jin-zhi
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Xin-yong
authors.initials: XY
authors.lastname: Ma
mesh_terms: D018663:Adrenergic Agents / Q000737:chemistry / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D029789:Ephedra / Q000737:chemistry*; D004809:Ephedrine / Q000737:chemistry* / Q000302:isolation & purification; D007536:Isomerism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D018663:Adrenergic Agents; D004365:Drugs, Chinese Herbal; D004809:Ephedrine