[Study on transplantation of marrow stromal stem cells into acellular extra cellular matrix in mice [corrected]].
OBJECTIVE
To study the integration of mice [corrected] marrow stromal stem cells (MSCs) after transplantation into acellular extracellular matrix (AECM).


METHODS
We got 16 femurs from 8 Kunming mice [corrected], the femurs were treated by Triton X-100 to get AECM, MSCs were collected from femoral marrow of 20 Kunming rats about a mouth old by PBS 4 ml, centrifuged and primary cultured in bottles, then the mice [corrected] MSCs were transplanted into AECM at a concentration of 5 x 10(6)/ml and cultured for 7 days. The integration of the donor cells was observed using one phase contrast microscope, a light microscope and a scanning electron microscope (SEM).


RESULTS
In AECM bone lacunas there were MSCs nuclei stained blue. The nuclei were unevenly distributed in AECM with more in the peripheral AECM than in the central AECM and with more in the layer near culture medium than in the layer far away from culture medium. AECM possessed a good spatial scaffold structure, the marrow stromal stem cells were well integrated into AECM.


CONCLUSIONS
AECM can be used as a good scaffold material for tissue engineered bone construction.
Dong
Hongming
H
Institute of Tissue Engineering, China Medical University, Shenyang Liaoning, 110001, PR China.
Bai
Shuling
S
D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods; D001857:Bone Matrix / Q000166:cytology / Q000601:surgery* / Q000648:ultrastructure; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D033581:Stem Cell Transplantation / Q000379:methods*; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation* / Q000648:ultrastructure; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous