[Analysis of contents of flavonoids in Mongolian medicine zhenbao pills II and III].
Both zhenbao pills II and III are Mongolian medicine of a kind. Zhenbao pill II was the artificial syntheses and zhenbao pill III was made from natural materials. In this paper, the flavonoids of Mongolian medicine zhenbao pills II and III were extracted by Soxhlet apparatus with methyl alcohol as extracting agent, the colorimetric method was applied to the determination of flavonoids, and the experimental procedure was studied with zhenbao pill II as the test sample. The result showed that the linear range of quantitative determination was 8.05-48.28 microg x mg(-1). The standard addition recovery (SAR) was 99.49%-100.50%. The RSD (n = 3) was 0.54%. The range of contents of flavonoids was 1.47-1.55 mg x g(-1) in zhenbao pill II and was 2.88-3.00 mg x g(-1) in zhenbao pill III. This method was simple and accurate with good reproducibility, and is suitable for the determination of flavonoids in all kinds of pills. The contents of flavonoids can be used to prove whether zhenbao pill is artificial syntheses or natural material.
Yun
Xue-ying
XY
Department of Pharmacy, Nei Mongol Medical College, Hohhot, China.
Chen
Qi-xiu
QX
Tong
Jie
J
Han
Yan-ping
YP
D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019411:Clinical Laboratory Techniques; D019281:Dimerization; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D019075:Electrophoresis, Capillary / Q000379:methods; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D056785:Medicine, Mongolian Traditional / Q000009:adverse effects; D000432:Methanol / Q000737:chemistry; D011786:Quality Control; D012015:Reference Standards; D012106:Research; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014466:Ultraviolet Rays / Q000009:adverse effects
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D000432:Methanol