[Development of a new type soybean germplasm with null lipoxygenase isozymes].
Soybean protein is a kind of high-quality protein composed of balanced amino acids. It contains all kinds of amino acids, especially eight amino acids necessary for human, but also contains some components that are not good for human and affect food quality, such as Lipoxygenase (Lox) and Trypsin inhibitor (Ti). Nutritional value and processing quality of soybean can be improved by means of development of new variety with null Lox and Ti. In this paper, a new type soybean germplasm with null lipoxygenase isozymes was developed by Institute of Crop Sciences, Chinese Academy of Agricultural Sciences through years of biochemical marker assistant selection for null lipoxygenase by means of isoelectric focusing-polyacrylamid gel electrophoresis (IEF-PAGE) in the hybrid progenies of "96P17" (Female parent, a null lipoxygenase 2.3 line) and "93704" (Male parent, a null lipoxygenase 1.3 line). It is the first new soybean germplasm with null Lox1.2.3 genes in our country, which will contribute to soybean breeding for high quality, soybean production and utilization. In this paper, the development process of new type soybean germplasm is described.
Han
Fen-Xia
FX
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Key Lab for Crop Genetics and Breeding of Ministry of Agriculture, Beijing 100081, China. hanfx@mail.caas.net.cn
Ding
An-Lin
AL
Sun
Jun-Ming
JM
Li
Gui-Ying
GY
D001947:Breeding / Q000379:methods*; D003433:Crosses, Genetic; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008084:Lipoxygenase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D013025:Glycine max / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
D007527:Isoenzymes; D008084:Lipoxygenase