title: Regulation of GluR1 abundance in murine hippocampal neurones by serum- and glucocorticoid-inducible kinase 3.
abstract: Phosphatidylinositol 3 kinase (PI3-kinase) is activated during and is required for hippocampal glutamate receptor-dependent long-term potentiation. It mediates the delivery of AMPA receptors to the neuronal surface. Among the downstream targets of PI3-kinase are three members of the serum- and glucocorticoid-inducible kinase family, SGK1, SGK2 and SGK3. In Xenopus oocytes expressing the AMPA subunit GluR1, we show that SGK3, and to a lesser extent SGK2, but not SGK1, increase glutamate-induced currents by increasing the abundance of GluR1 protein in the cell membrane. We further show Sgk3 mRNA expression in the hippocampus by RT-PCR and in situ hybridization. According to Western blotting, the hippocampal abundance of GluR1 is significantly lower in gene-targeted mice lacking SGK3 (Sgk3-/-) than in their wild-type littermates (Sgk3+/+). The present observations disclose a novel mechanism in the regulation of GluR1.
authors.affiliation: Department of Physiology I, University of Tuebingen, 72076 Tuebingen, Germany.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Strutz-Seebohm
authors.forename: Guiscard
authors.initials: G
authors.lastname: Seebohm
authors.forename: Andreas F
authors.initials: AF
authors.lastname: Mack
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Wagner
authors.forename: Lothar
authors.initials: L
authors.lastname: Just
authors.forename: Thomas
authors.initials: T
authors.lastname: Skutella
authors.forename: Undine E
authors.initials: UE
authors.lastname: Lang
authors.forename: Guido
authors.initials: G
authors.lastname: Henke
authors.forename: Marion
authors.initials: M
authors.lastname: Striegel
authors.forename: Michael
authors.initials: M
authors.lastname: Hollmann
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rouach
authors.forename: Roger A
authors.initials: RA
authors.lastname: Nicoll
authors.forename: James A
authors.initials: JA
authors.lastname: McCormick
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Pearce
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006624:Hippocampus / Q000166:cytology*; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009474:Neurons / Q000201:enzymology*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018091:Receptors, AMPA / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D017874:Immediate-Early Proteins; D007527:Isoenzymes; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D018091:Receptors, AMPA; D000099184:Serum-Glucocorticoid Regulated Kinases