[Quantification of human ermap by using real-time FQ-PCR].
To develop a real-time FQ-PCR method for quantifying human ermap, a set of primers and a fluorescent probe were designed by primer express 2.0. pBluescriptSK(+) plasmid contained ermap cDNA was transcribed to generate calibration standards for quantification. A real time FQ-PCR method was established. The results showed that when the concentrations of DNA to be amplified were ranged from 1.725 x 10(7) to 1.725 x 10(10) cps/ml, there was a good correlation between template concentration and cycle threshold, and the correlation coefficient reached to -0.999376. In conclusion, real time FQ-PCR which is specific, sensitive and accurate can be used to further research on human ermap.
Zhang
Xiao-Hong
XH
Department of Pediatrics, Conghua Central Hospital, Guangzhou 510089, China.
Ye
Tie-Zhen
TZ
Hu
Bin
B
Si
Wen-Zhang
WZ
D001789:Blood Group Antigens / Q000235:genetics*; D000071797:Butyrophilins; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
D001789:Blood Group Antigens; D000071797:Butyrophilins; D018076:DNA, Complementary; D005456:Fluorescent Dyes; C472715:scianna antigens