title: Identifying relapsing fever Borrelia, Senegal.
abstract: We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers. This technique, based on single nucleotide polymorphisms of the 16S ribosomal RNA gene, was used to test blood samples from 7,750 patients, 33 of whom were diagnosed with spirochete infections. Borrelia crocidurae was the only species identified.
authors.affiliation: Institut Pasteur, Dakar, Senegal.
authors.forename: Hamoud
authors.initials: H
authors.lastname: Brahim
authors.forename: Jean David
authors.initials: JD
authors.lastname: Perrier-Gros-Claude
authors.forename: Danielle
authors.initials: D
authors.lastname: Postic
authors.forename: Guy
authors.initials: G
authors.lastname: Baranton
authors.forename: Ronan
authors.initials: R
authors.lastname: Jambou
mesh_terms: D000328:Adult; D001898:Borrelia / Q000145:classification / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012061:Relapsing Fever / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D012675:Senegal / Q000453:epidemiology; D013045:Species Specificity