Cost-effective production of Bacillus thuringiensis by solid-state fermentation.
Production of Bacillus thuringiensis (Bt) was standardized on wheat bran based media in 250 ml Erlenmeyer flasks. Scale-up of Bt production on the best medium in plastic tubs with aeration at 8 h intervals starting 16 h after incubation yielded a significant increase in spore count and toxin content of the product. Maximum lysis of Bt cells was obtained by 60 h of incubation at 30 degrees C. This protocol was suitable for production of Bt strains and local isolates. The Bt produced proved highly effective at 0.1% concentration against larvae of castor semilooper, Achaea janata L, resulting in complete mortality by three days in laboratory bioassays. In field trials, the population of castor semilooper larvae on the castor bean crop was reduced significantly by three days after application. The cost for material production of 1 kg of Bt was approximately US dollars 0.70.
Devi
P S Vimala
PS
Directorate of Oilseeds Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India. psvdevi@yahoo.co.in
Ravinder
T
T
Jaidev
C
C
D000818:Animals; D001413:Bacillus thuringiensis / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000191:economics* / Q000379:methods*; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry*; D004043:Dietary Fiber; D005285:Fermentation; D007218:Industrial Microbiology / Q000191:economics* / Q000592:standards; D008965:Molasses
D003470:Culture Media; D004043:Dietary Fiber