Functional characterizations of novWUS involved in novobiocin biosynthesis from Streptomyces spheroides.
NovW, novU, and novS gene products represent dTDP-4-keto-6-deoxy-D-glucose 3,5 epimarase, C-methyltransferase and dTDP-glucose-4-ketoreductase involved in noviose biosynthetic pathway, respectively. We have expressed three genes to elucidate the functions of NovW, NovU, and NovS in Escherichia coli. NovW and NovU catalyze the formation of dTDP-4-keto-6-deoxy-5-C-methyl-L-lyxo-hexose from dTDP-4-keto-6-deoxy-D-glucose. NovS reduces the product formed from the reaction of NovW with dTDP-4-keto-6-deoxy-D-glucose in the presence of NADH to result in dTDP-l-rhamnose. Furthermore, a pathway for the biosynthesis of noviose is proposed.
Thuy
Ta Thi Thu
TT
Institute of Biomolecule Reconstruction (iBR), Department of Chemistry, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.
Lee
Hei Chan
HC
Kim
Chun-Gyu
CG
Heide
Lutz
L
Sohng
Jae Kyung
JK
D001483:Base Sequence; D002238:Carbohydrate Epimerases / Q000145:classification / Q000378:metabolism / Q000494:pharmacology*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003837:Deoxy Sugars / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D008956:Models, Chemical; D005810:Multigene Family; D009243:NAD / Q000494:pharmacology; D009675:Novobiocin / Q000096:biosynthesis* / Q000737:chemistry; D009702:Nucleoside Diphosphate Sugars / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000737:chemistry / Q000378:metabolism
D003837:Deoxy Sugars; D009702:Nucleoside Diphosphate Sugars; D013942:Thymine Nucleotides; D009243:NAD; D009675:Novobiocin; C073293:thymidine diphosphate rhamnose; D002238:Carbohydrate Epimerases; C483836:NovW Carbohydrate Epimerase