Growth characteristics of canine distemper virus in a new cell line CCT cells originated from canine malignant histiocytosis.
Canine distemper virus (CDV) growth and the morphological characterization were examined in a cell line established from a canine malignant histiocytosis (CCT cell line). The susceptibility of the CCT cells to 3 CDV strains, FXNO, YSA-TC and MD-77 was shown by detection of the antigen in the indirect fluorescent assay. After passaging 4 and 9 times through the CCT cells, only FXNO strain could produce the syncytia where demonstrated the antigens. Titers of 9 passaged viruses through the CCT cells showed slightly higher in the CCT cells than those in Vero cells. Morphological characterization of karyorrhexis and specific DNA ladder by extracted DNA electrophoresis indicated apoptosis in the CDV infected CCT cells.
Yamaguchi
Ryoji
R
Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Kojimoto
Atsuko
A
Sakai
Hiroki
H
Uchida
Kazuyuki
K
Sugano
Sumio
S
Tateyama
Susumu
S
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053938:DNA Fragmentation / Q000235:genetics; D004217:Distemper Virus, Canine / Q000254:growth & development*; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006644:Histiocytes / Q000166:cytology* / Q000821:virology; D013045:Species Specificity; D014407:Tumor Cells, Cultured