Application of a new chemiluminescence method for the determination of glucose-6-phosphate dehydrogenase activity in healthy and enzyme-deficient individuals.
BACKGROUND
A chemiluminescence (CL) technique, which determines the glucose-6-phosphate dehydrogenase (G-6-PD) activities in healthy, heterozygous, and completely enzyme-deficient individuals was applied.


METHODS
CL intensities were detected for 4 h at 15-min intervals in each sample with or without addition of G-6-PD substrates into the reaction mixture.


RESULTS
The results revealed an inverse correlation to the reference UV method (Zinkham method; r=-0.80). Furthermore, the CL assay was able to detect G-6-PD activities as low as 0.2 IU/gHb, which was not possible by the UV method.


CONCLUSIONS
In conclusion, we believe that this method offers a new diagnostic tool for the detection of G-6-PD activities in enzyme-deficient individuals and, because of its increased sensitivity, makes it amenable for determining the effects of different pharmaceutical agents on G-6-PD activity in tissue or cell cultures.
Gumuslu
Saadet
S
Akdeniz University, School of Medicine, Department of Biochemistry, 07070, Antalya, Turkey.
Yucel
Gultekin
G
Sarikcioglu
Sureyya Bilmen
SB
Serteser
Mustafa
M
D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood* / Q000235:genetics*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood* / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005954:Glucosephosphate Dehydrogenase