Capacity of CD34+ progenitor-derived dendritic cells to distinguish between sensitizers and irritants.
In an attempt to develop an in vitro test to identify contact sensitizers, mostly dendritic cells (DCs) derived from monocytes (Mo-DC) have been used. Less is known about the potency of DC derived from CD34+ progenitors (CD34-DC) for in vitro allergen testing. CD34+ progenitor derived DC were exposed to nine well-known allergens (one weak, three moderate and five strong allergens) and two irritants. Surface marker expression (CD86, CD83 and HLA-DR) and cytokine production (IL-6, IL-12 and TNF-alpha) were analyzed after 24 h exposure to these chemicals. All allergens tested induced a significant increase in at least one of the DC surface markers. In contrast, none of the irritants tested were able to significantly upregulate membrane marker expression in exposed DC. The level of upregulation of CD86, CD83 and HLA-DR was dependent on the nature and concentration of the chemical, but not on the classification of the allergen. Changes in cytokine production (IL-6, IL-12 and TNF-alpha) were not consistently related to exposure to an allergen. Based on these results, we conclude that the in vitro test using CD34-DC has the capacity to distinguish between allergens and irritants based on altered phenotypic characteristics.
De Smedt
Ann C A
AC
Vito (Flemish Institute for Technological Research), Boeretang 200, 2400 Mol, Belgium. adsmedt1@prdbe.jnj.com
Van Den Heuvel
Rosette L
RL
Van Tendeloo
Vigor F I
VF
Berneman
Zwi N
ZN
Schoeters
Greet E R
GE
D000485:Allergens / Q000494:pharmacology*; D018952:Antigens, CD34 / Q000096:biosynthesis*; D015415:Biomarkers / Q000032:analysis; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007509:Irritants / Q000494:pharmacology*; D010641:Phenotype
D000485:Allergens; D018952:Antigens, CD34; D015415:Biomarkers; D016207:Cytokines; D007509:Irritants