Fastidiosipila sanguinis gen. nov., sp. nov., a new Gram-positive, coccus-shaped organism from human blood.
Phenotypic and phylogenetic studies were performed on two strains of an unidentified Gram-positive, fastidious, non-spore-forming, coccus-shaped bacterium recovered from human blood. The organism was catalase-negative and grew under strictly anaerobic conditions and in the presence of 2 and 6 % O(2). Comparative 16S rRNA gene sequencing demonstrated that the unidentified bacterium was, phylogenetically, far removed from peptostreptococci and related Gram-positive coccus-shaped organisms, but exhibited a phylogenetic association with Clostridium rRNA cluster III [as defined by Collins et al., Int J Syst Bacteriol 44 (1994), 812-826]. Sequence divergence values of 15 % or more were observed between the unidentified bacterium and all other recognized species within this and related rRNA clostridial clusters. Treeing analysis showed that the unknown bacterium formed a deep line branching at the periphery of rRNA cluster III and represents a hitherto unknown genus within this supra-generic grouping. On the basis of both phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from blood be classified in a new genus, Fastidiosipila gen. nov., as Fastidiosipila sanguinis sp. nov. The type strain of Fastidiosipila sanguinis is CCUG 47711(T) (=CIP 108292(T)).
Falsen
Enevold
E
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Sweden.
Collins
Matthew D
MD
School of Food Biosciences, University of Reading, Reading, UK.
Welinder-Olsson
Christina
C
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Sweden.
Song
Yuli
Y
Infectious Diseases Section, VA Medical Center, West Los Angeles, CA 90073, USA.
Finegold
Sydney M
SM
Department of Medicine, UCLA School of Medicine, CA 90073, USA.|Infectious Diseases Section, VA Medical Center, West Los Angeles, CA 90073, USA.
Lawson
Paul A
PA
School of Food Biosciences, University of Reading, Reading, UK.
D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006095:Gram-Positive Cocci / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S