Exhaustive exercise and the cellular stress response in rainbow trout, Oncorhynchus mykiss.
The goal of this study was to examine the cellular response to exhaustive exercise in male and female rainbow trout to determine if HSPs are involved in the early stages of the recovery process. Levels of HSPs and key metabolic parameters were measured in white muscle, heart plasma, and blood plasma throughout 6 h of recovery from exhaustive burst exercise. Plasma creatine kinase (CK) was also quantified as an indicator of exercise-induced tissue damage. The observed trends in ATP and lactate were consistent with established patterns of exhaustion and the beginnings of metabolic recovery. However, no upregulation of hsp70, hsp30, or hsp90 was evident in heart or muscle tissue of males or females, and plasma CK measurements suggest that tissue damage was minimal. Our results indicate that hsp70, hsp30, and hsp90 are not part of the early recovery process from burst exercise in fish, perhaps due to the maintenance of core temperatures as well as a lack of exercise-induced tissue damage.
Clarkson
Kathleen
K
Department of Biology, Mount Allison University, Sackville, NB, Canada E4L 1G7.
Kieffer
James D
JD
Currie
Suzanne
S
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003402:Creatine Kinase / Q000097:blood; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012737:Sex Factors
D001786:Blood Glucose; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D000255:Adenosine Triphosphate; D003402:Creatine Kinase