title: [Transient cytosolic free calcium changes in cultured neonatal rat cardiac myocytes in response to advanced glycation end-product treatment].
abstract: OBJECTIVE
  To investigate the effects of advanced glycation end-products (AGEs) on transient cytosolic free calcium in neonatal rat cardiac myocytes (CMs) cultured in vitro.
  
  
  METHODS
  CMs cultured for 3 to 5 days in vitro were incubated with Ca(2+)-sensitive fluorescent indicator Fluo-3AM with light screening at 37 degrees celsius; with 5% CO(2) for 60 min. Changes of the fluorescence signal of free calcium caused by AGEs were measured under laser scanning confocal microscope (LSCM).
  
  
  RESULTS
  Compared with the control cells, AGEs caused an increase in the concentration of cytosolic free calcium in a dose-dependent manner.
  
  
  CONCLUSIONS
  AGEs may impair neonatal rat CMs by altering cytosolic free calcium concentration.
authors.affiliation: Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Shao
authors.forename: Ding-Li
authors.initials: DL
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Lai
authors.forename: Hao
authors.initials: H
authors.lastname: Ren
authors.forename: Rong-Yan
authors.initials: RY
authors.lastname: Jiang
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D007202:Indicators and Reagents; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D015220:Calcium Channels; D017127:Glycation End Products, Advanced; D007202:Indicators and Reagents; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium