Plasmodium vivax: allele variants of the mdr1 gene do not associate with chloroquine resistance among isolates from Brazil, Papua, and monkey-adapted strains.
We describe here the sequence of the Plasmodium vivax mdr1 gene from 10 different isolates differing in chloroquine sensitivity. The deduced amino acid sequence of PvMDR1 shares more than 70% similarity with other malarial MDR proteins and it displays consensus motifs of an ABC family transporter including two transmembrane domains and two ATP binding cassettes. Similarity and dendrogram analyses revealed that sequences could be grouped according to their geographical origin. Within each geographical group however, no correlation was found between chloroquine resistance and specific mutations.
Sá
Juliana Martha
JM
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Lineu Prestes 1374, São Paulo SP, 05508-900, Brazil.
Nomura
Takashi
T
Neves
Joana d'Arc
Jd
Baird
J Kevin
JK
Wellems
Thomas E
TE
del Portillo
Hernando A
HA
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000737:chemistry / Q000235:genetics*; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D001938:Brazil; D002738:Chloroquine / Q000494:pharmacology*; D016384:Consensus Sequence; D017124:Conserved Sequence; D004351:Drug Resistance / Q000235:genetics; D019450:Genes, MDR / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D000882:Haplorhini; D006801:Humans; D008969:Molecular Sequence Data; D010219:Papua New Guinea; D010802:Phylogeny; D010966:Plasmodium vivax / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D017354:Point Mutation; D016415:Sequence Alignment
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000962:Antimalarials; D002738:Chloroquine