[Study on spectral identification of methamidophos artificial antigen].
In this paper, spectral identification of methamidophos artificial antigen was reported. The results showed that UV absorbance spectrum of artificial antigens of BSAM changed compared with carrier BSA and hapten Met. The absorbance peaks which are similar to P-O-C and P=O moiety in IR spectrum of Met molecule appeared in the IR spectrum of the artificial antigens of BSAM. The chemical shift of the artificial antigens of BSAM is the same as that of standard Met in 31P NMR spectrum. Phosphor concentration of the artificial antigens of BSAM was determined by phosphor molybdate blue spectrophotometry, and then conjugating ratio was calculated, to be 11. The results obtained with the above four spectrum methods showed that the artificial antigens of BSAM was synthesized successfully.
Zhao
Su-qing
SQ
Department of Food Science, South China Agriculture University, Guangzhou 510642, China.
Cai
Yan-fei
YF
Lei
Hong-tao
HT
Sun
Yuan-ming
YM
Huang
Xiao-yu
XY
Zhang
Hou-rui
HR
Zhu
Zhen-yu
ZY
D000941:Antigens / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009946:Organothiophosphorus Compounds / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013053:Spectrophotometry / Q000379:methods*; D013055:Spectrophotometry, Infrared / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods; D014614:Vaccines, Synthetic / Q000276:immunology*
D000941:Antigens; D006241:Haptens; D009946:Organothiophosphorus Compounds; D014614:Vaccines, Synthetic; D012710:Serum Albumin, Bovine; C014655:methamidophos