title: Minimum conditions of major histocompatibility complex compatibility and recipient immune compromise required to establish donor white blood cell persistence in a murine transfusion model.
abstract: BACKGROUND
  In some patients multiply transfused to treat severe trauma, white blood cells (WBCs) from a single blood donor can persist for years, constituting up to 5 percent of all circulating WBCs. The immunologic mechanisms responsible for this are not known but, if understood, might allow manipulation of the human immune system to induce microchimerism for a variety of therapeutic purposes. To better characterize these mechanisms, a murine transfusion model was developed with a panel of immunologic knockouts as transfusion recipients. By conducting a systematic series of transfusion experiments, the purpose was to determine which recipient immune cell population, when abrogated, could lead to prolonged survival of donor cells (microchimerism).
  
  
  METHODS
  Blood was transfused from normal donors to knockout recipients in syngeneic, allogeneic, and xenogeneic settings. Donor WBC survival was evaluated by quantitative polymerase chain reaction, and recipient lymphocyte subsets by fluorescence-activated cell sorting.
  
  
  RESULTS
  In the syngeneic setting, donor WBCs persisted in C2ta, RAG-1, and TCR knockout recipients. Allogeneic donor WBCs persisted in RAG-2 and RAG-2/Common gamma knockout recipients. Xenogeneic donor WBCs required RAG-2/Common gamma and RAG-2/Pfp double knockouts to persist.
  
  
  CONCLUSIONS
  It is concluded that donor-recipient major histocompatibility complex (MHC) concordance alone is not sufficient to achieve microchimerism. Further, the degree of recipient immune compromise necessary to achieve persistent microchimerism is directly proportional to the degree of donor-recipient MHC disparity.
authors.affiliation: Department of Molecular Biology at the Blood Systems Research Institute, Blood Systems, Inc, San Francisco, California 94118, USA.
authors.forename: Tzong-Hae
authors.initials: TH
authors.lastname: Lee
authors.forename: Li
authors.initials: L
authors.lastname: Wen
authors.forename: Lani
authors.initials: L
authors.lastname: Montalvo
authors.forename: Olubukola
authors.initials: O
authors.lastname: Esho
authors.forename: Cliff
authors.initials: C
authors.lastname: Lowell
authors.forename: William
authors.initials: W
authors.lastname: Reed
authors.forename: Michael
authors.initials: M
authors.lastname: Busch
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D002678:Chimera; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics; D017708:Leukocyte Transfusion; D007962:Leukocytes / Q000166:cytology / Q000528:radiation effects; D008285:Major Histocompatibility Complex / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein