[Optimization of RT-PCR for the identification of VP4 gene of group A rotaviruses and evaluation of its diagnostic efficiency].
A method is suggested for the detection of the human rotavirus VP4 gene by RT-PCR. The method is universal for all [P] types of group A rotavirus in clinical samples. As against RNA electrophoresis and ELISA, it provides for a more accurate detection of rotaviruses by 18.8 and 26.5%, respectively. It was established by RT-PCR in February, 2002, in Nizhny Novgorod, that rotaviruses were responsible for 67% of acute gastroenteritis in hospitalized children.
Fedorova
O F
OF
Novikova
N A
NA
Epifanova
N V
NV
Lukovnikova
L B
LB
Kniagina
O N
ON
Gracheva
E V
EV
Bogacheva
E M
EM
D036022:Capsid Proteins / Q000032:analysis / Q000235:genetics*; D002648:Child; D017931:DNA Primers; D003937:Diagnosis, Differential; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D036022:Capsid Proteins; D017931:DNA Primers; C060339:VP4 protein, Rotavirus