Productive infection of primary murine astrocytes, lymphocytes, and macrophages by human immunodeficiency virus type 1 in culture.
A mouse model of human immunodeficiency virus type 1 (HIV-1) infection would be extremely valuable for evaluation of therapies and vaccines; however, multiple blocks to productive infection of NIH 3T3 and other mouse cell lines have been reported. The authors investigated the replication of HIV-1 in primary mouse astrocytes, lymphocytes, and macrophages in culture by infection with intact HIV-1 pseudotyped with the vesicular stomatitis virus G envelope glycoprotein (VSV-G) or with the envelope glycoprotein of amphotropic murine leukemia virus. Astrocytes, lymphocytes, and macrophages were susceptible to productive infection as variously assayed by detection of p24 and Tat proteins, viral protease-mediated processing of Gag, appropriately spliced viral RNA, and infectious progeny virus. As expected, NIH 3T3 cells were not susceptible to productive infection by VSV/NL4. Susceptibility mapped neither to the Fv locus nor to a possible polymorphism in cyclin T1. This study indicates that there are no intrinsic intracellular barriers to HIV-1 replication in primary mouse cells when virus entry is efficient.
Nitkiewicz
Jadwiga
J
Molecular Virology Division, St Luke's-Roosevelt Hospital Center, New York, New York 10019, USA.
Chao
Wei
W
Bentsman
Galina
G
Li
Jinliang
J
Kim
Seon-Young
SY
Choi
So Young
SY
Grunig
Gabrielle
G
Gelbard
Harris
H
Potash
Mary Jane
MJ
Volsky
David J
DJ
D000818:Animals; D001253:Astrocytes / Q000821:virology*; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D016655:HIV Core Protein p24 / Q000378:metabolism; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D008214:Lymphocytes / Q000821:virology*; D008264:Macrophages / Q000821:virology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D012326:RNA Splicing; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000378:metabolism; D014779:Virus Replication / Q000502:physiology
C028391:G protein, vesicular stomatitis virus; D016655:HIV Core Protein p24; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins