[Effects of CD151 on migration of human tongue squamous carcinoma cell line Tca8113].
OBJECTIVE
Over-expression of CD151 gene in tumor tissues may be associated with metastasis and poor prognosis of cancer, but the mechanism is unknown. This study was designed to determine the effects of CD151 gene on migration of human tongue squamous carcinoma cell line Tca8113.


METHODS
Functional fragment of CD151 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and inserted into plasmid adeno-associated virus (pAAV) vector in sense direction and antisense direction to construct rAAV-CD151 and rAAV-antiCD151, the virus titers were determined by dot blot. Two weeks after transfection, protein level of CD151 in Tca8113 cells was detected by Western blot, the effect of CD151 on migration of Tca8113 cells was detected by Transwell chambers.


RESULTS
Titer of rAAV-CD151 was 2x10(11) pfu/ml, titer of rAAV-antiCD151 was 1x10(11) pfu/ml. Transfection of rAAV-CD151 increased expression of CD151 in Tca8113 cells by 108% of control cells, while transfection of rAAV-antiCD151 decreased expression of CD151 in Tca8113 cells by 79% of control cells. Migrated cells count was significantly higher in rAAV-CD151-transfected cells (93.6+/-11.6), and lower in rAAV-antiCD151-transfected cells (24.0+/-4.4) than in untransfected and rAAV-GFP-transfected cells (53.0+/-6.6 and 46.0+/-7.0, P<0.05).


CONCLUSIONS
It is an important molecular mechanism of tumor metastasis that over-expression of CD151 promotes migration of tumor cells. rAAV-antiCD151, a novel therapeutic tool, can specifically reduce the expression of CD151, and inhibit migration of tumor cells.
Lan
Rong-Fang
RF
Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P.R.China.
Liu
Zheng-Xiang
ZX
Song
Yu-E
YE
Zhang
Xin
X
Wang
Dao-Wen
DW
D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D016373:DNA, Antisense / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D060185:Tetraspanin 24; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
D015703:Antigens, CD; C554715:CD151 protein, human; D016373:DNA, Antisense; D060185:Tetraspanin 24