title: [Oligonucleotide microarray for human immunodeficiency virus detection].
abstract: OBJECTIVE
  To develop an oligonucleotide microarray for fast detection of human immunodeficiency virus (HIV).
  
  
  METHODS
  With complete genome sequence of HIV-1 subtype B (U26942) as the target sequence and bioinformatics software such as DNAClub, Oligo6.0, BLAST, Alignment, oligonucleotide probes of high specificity with identical length and similar melting temperature (T(m)) were designed and synthesized. Oligonucleotide microarray was prepared using Cartesian Microarrayer. Using the plasmids of HIV-1 subtype B(U26942), C(U46016), F(AF075703), G(AF061640) and restriction display technique, Cy3-labeled HIV DNA fragments were amplified and the hybridization results were scanned and analyzed with Array-Pro.
  
  
  CONCLUSIONS
  Twenty-two optimized oligonucleotide microarray probes were obtained and used to prepare the oligonucleotide microarray for further screening studies. The microarray prepared significantly enhanced the sensitivity, reliability and speed of DNA assay, and possesses the potential for application in clinical setting.
authors.affiliation: Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. wenwen97@fimmu.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Wen
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Wu
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Xu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhang
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D001483:Base Sequence; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity