[A sort of meliorative method for testing DNA from sperm smear].
OBJECTIVE
To establish an effective method for testing DNA from the sperm smear.


METHODS
We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA. The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit.


RESULTS
Although there were a few sperm cells on the slide, it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction.


CONCLUSIONS
The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case.
Sun
Yao-dong
YD
Institute of Criminal Science,Public Security Bureau of Yancheng City, Yancheng 224002, China.
Ren
Hong
H
Chen
Yue-yong
YY
D000328:Adult; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011902:Rape; D013048:Specimen Handling; D013094:Spermatozoa / Q000737:chemistry*; D020080:Tandem Repeat Sequences / Q000235:genetics
D004247:DNA