Kinetics and distribution of bovine gammadelta T-lymphocyte in the intestine: gammadelta T cells accumulate in the dome region of Peyer's patch during prenatal development.
The kinetics and distribution of gammadelta T cells in bovine intestine including jejunal and ileal Peyer's patch were examined. The number of gammadelta T cells increased significantly in the dome region during prenatal development, but decreased notably after birth. The number of some gammadelta T cells, CD4+ cells, and CD8+ cells in the intestinal villi remained constant during prenatal development, but increased significantly after birth. The kinetics of the gammadelta T cells in the dome region during prenatal development were quite distinct from those of the gammadelta T cells, CD4+ cells, and CD8+ cells in the intestinal villi. In the fetal ileum at full-term gestation, the frequencies of expression of the T-cell receptor gamma variable region (TCR Vgamma) family were TCR Vgamma1 (48%), Vgamma2 (4%), and Vgamma5 (48%). However, in 2-month-old calf ileum, TCR Vgamma5 (90%) was dominant. We speculate that functional differences exist between gammadelta T cells in the dome region during prenatal development and in the intestinal villi after birth.
Yasuda
Masahiro
M
Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan. yasudaja@cc.miyazaki-u.ac.jp
Ogawa
Daisuke
D
Nasu
Tetsuo
T
Yamaguchi
Takahiro
T
Murakami
Takayuki
T
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002417:Cattle; D005260:Female; D047109:Fetal Development / Q000276:immunology*; D005333:Fetus / Q000276:immunology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007135:Immunoglobulin Variable Region / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D007422:Intestines / Q000166:cytology / Q000276:immunology*; D007700:Kinetics; D010581:Peyer's Patches / Q000166:cytology / Q000196:embryology* / Q000276:immunology*; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D007135:Immunoglobulin Variable Region; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012313:RNA