[Enzyme-linked immunosorbent assay biotin/avidin method standardization, for identification of Lutzomyia (Lutzomyia) longipalpis bloodmeals (Lutz & Neiva, 1912)].
Bloodmeals taken by insects constitute an important parameter for clarifying aspects of the transmission of zoonoses, including leishmaniases. Immunological assays can be used to investigate the attraction of vectors to animals, which may be hosts of these parasitoses. The objective of this study was to standardize a sandwich enzyme-linked immunosorbent assay and titer samples with different time periods of digestion, in laboratory-bred Lutzomyia longipalpis fed on rats. In the light of the high sensitivity that the biotin-avidin method permits, the technique provided at least ninety repeat tests for each sample and identified recent bloodmeals taken by these insects. Bloodmeals were detectable up to 12 and 24 h after blood ingestion, and a significant difference between these titers was observed.
Marassá
Ana Maria
AM
Instituto Adolfo Lutz, São Paulo, SP.
Consales
Cleide Aschenbrenner
CA
Galati
Eunice Aparecida Bianchi
EA
D000818:Animals; D001360:Avidin; D001710:Biotin; D001769:Blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005260:Female; D007303:Insect Vectors; D008297:Male; D011233:Precipitin Tests / Q000379:methods; D011576:Psychodidae; D051381:Rats; D012680:Sensitivity and Specificity; D013997:Time Factors
D001360:Avidin; D001710:Biotin