[Determination of deoxyribonuceic acids by resonance light scattering technique with benzidine in the presence of the sensitizing sodium dodecyl sulfate].
The resonance light scattering (RLS) spectra of benzidine with deoxyribonucleic acid (ctDNA) in the presence of Sodium Dodecyl Sulfate had been studied. RLS intensity of SDS-benzidine system was enhanced by trace ctDNA and was proportional to the concentrationof ctDNA. Under optima conditions, the linear ranges of the calibration were 0.3-4 mg x L(-1), the linear regression equation was I = 37.17c (ctDNA, mg x L(-1)) + 208.9, r = 0.9992. The limit of detection were 0.17 mg x L(-1) (3delta), RSD% = 3.5%. The method had been applied to the determination of DNA in synthetic samples and gene tobacco sample, and the results were satisfactory.
Rao
Zhen-hong
ZH
College of Science, China Agricultural University, Beijing 100094, China.
Xiong
Yan-mei
YM
Ren
Li-ping
LP
Jiang
Shu-ren
SR
Zhang
Chun-rong
CR
D001560:Benzidines / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D008027:Light; D057230:Limit of Detection; D012542:Scattering, Radiation; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001560:Benzidines; C029876:benzidine; D012967:Sodium Dodecyl Sulfate; D004247:DNA