A method to circumvent the lot number of activated carbon affecting the performance of activated carbon silica gel columns used for cleanup of blood samples for analysis of 29 hazardous organochlorine compounds.
We have previously described the use of a tandem simplified multilayer silica gel-activated carbon dispersed silica gel (TS-ML-AC) column for the cleanup of blood samples for the analysis of 29 hazardous organochlorine compounds (OCs)--the 17 major polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/DFs) and 4 non-ortho- and 8 mono-ortho-polychlorinated biphenyls (PCBs). We noted that the performance of the activated carbon-silica gel (ACS) column (lower column) varied with the lot number of the ACS. In this study, we compared the elution profiles of OCs eluted on 5 ACS columns, each with a different ACS lot number, and found that only mono-ortho-PCBs #114 and #123 were affected by lot number. The problem was that the 50 ml of n-hexane required to elute all the OCs from the simplified multilayer silica gel (MLS) column (upper column) into the AC column (lower column) also eluted varying amounts of PCBs #114 and #123 from the ACS column by ACS lot number. Although we could prevent PCBs #114 and #123 from being eluted from the ACS column by reducing the n-hexane volume to 10 ml, this volume was not sufficient to elute all the OCs from the MLS column. We solved this by separating the two columns; the sample solution was eluted with 50 ml of n-hexane from the MLS column, this eluate was concentrated to about 0.3 ml using a rotary evaporator, and then the concentrated solution was cleaned up on the ACS column. The recovery rates of #114 and #123 from blood samples were above 70% and the relative standard deviations of their concentration were below 10%, irrespective of the lot number, compared with recovery rates of 45-79% for #114 and 59-89% for #123, and relative standard deviations of their concentration above 15% when 50 ml of n-hexane was run through the tandem column. Our modified method affords reliable and reproducible cleanup of blood samples for analysis of 29 OCs, irrespective of the ACS lot number.
Kitamura
Kimiyoshi
K
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan. kitamura.kimiyoshi@nies.go.jp
Takazawa
Yoshikatsu
Y
Hashimoto
Shunji
S
Choi
Jae-Won
JW
Ito
Hiroyasu
H
Morita
Masatoshi
M
D002606:Charcoal; D002850:Chromatography, Gel / Q000379:methods; D004785:Environmental Pollutants / Q000097:blood*; D015386:Hazardous Substances / Q000097:blood*; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000097:blood*; D012680:Sensitivity and Specificity; D058428:Silica Gel; D012822:Silicon Dioxide
D004785:Environmental Pollutants; D015386:Hazardous Substances; D006843:Hydrocarbons, Chlorinated; D002606:Charcoal; D058428:Silica Gel; D012822:Silicon Dioxide