Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa.
A cell line of murine osteogenic progenitor cells, Kusa, was established from femoral bone marrow stromal cells with other types of mesenchymal progenitor cells. We characterized two sublines of Kusa (Kusa-A1 and Kusa-O) from several aspects, including the use of an expression profiling system, a cDNA microarray. The original Kusa subline (Kusa-A1) had high alkaline phosphatase activity and high accumulation of calcium deposits in a condition inducing mineralization, with ascorbic acid and beta-glycerophosphate. Kusa-O, a low osteogenic subline of Kusa, had high alkaline phosphatase activity but slow accumulation of calcium deposits even in the inducing condition. These two Kusa sublines differed in the expression of the osteogenic marker genes, osteocalcin and osteopontin, during mineralization. A type of cDNA microarray revealed marked downregulation of gene expression in the inducing condition in both Kusa-A1 and Kusa-O. Another type of high-throughput microarray was performed to examine the difference in gene expression patterns between Kusa-A1 and Kusa-O. By this analysis, periostin, which would be involved in a stage of osteogenesis, was low in Kusa-A1. On the contrary, Myocyte enhancer factor 2C (MEF2C), a myogenic transcriptional factor, was high in Kusa-A1, although no expression of any other myogenic genes was shown.
Kawashima
Nobuyuki
N
Pulp Biology and Endodontics, Graduate School of Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan. kawashima.n.endo@tmd.ac.jp
Shindo
Kentaro
K
Sakamoto
Kei
K
Kondo
Hisatomo
H
Umezawa
Akihiro
A
Kasugai
Shohei
S
Perbal
Bernard
B
Suda
Hideaki
H
Takagi
Minoru
M
Katsube
Ken-ichi
K
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002460:Cell Line; D020869:Gene Expression Profiling; D064326:MEF2 Transcription Factors; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D018006:Myogenic Regulatory Factors / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000502:physiology*; D053495:Osteopontin; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; D064326:MEF2 Transcription Factors; C578041:Mef2c protein, mouse; D018006:Myogenic Regulatory Factors; C497762:Notch1 protein, mouse; C082838:Postn protein, mouse; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D014157:Transcription Factors; D015675:Osteocalcin; D053495:Osteopontin; D000469:Alkaline Phosphatase; D002118:Calcium