title: A tetracycline-regulatable adeno-associated virus vector for double-gene transfer.
abstract: An increasing demand for polycistronic vectors that express multiple genes simultaneously has arisen in recent years to obtain an efficient gene therapy. Armed with the knowledge that the expression of transgene in mammalian cells often requires tight control, we constructed in this study a tetracycline-regulated double-gene adeno-associated virus (AAV) vector carrying green and red fluorescent protein genes and expressed it in PC12 cells. When detected by fluorescence microscope and fluorescence-activated cell sorting, gene expression was induced by 44-66-fold and could be reversibly controlled by doxycycline. This double-gene AAV vector may be useful for regulated expression of two genes or a marker to monitor transgene expression.
authors.affiliation: Neuroscience Research Institute, Peking University, Beijing 100083, PR China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Dong-bin
authors.initials: DB
authors.lastname: Niu
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Xue
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D013752:Tetracycline / Q000494:pharmacology*; D014162:Transfection / Q000379:methods; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D013752:Tetracycline; D000097573:Red Fluorescent Protein