title: Vitamin E and gene expression in immune cells.
abstract: Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two-fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.
authors.affiliation: Nutritional Immunology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111, USA. sungnim.han@tufts.edu
authors.forename: Sung Nim
authors.initials: SN
authors.lastname: Han
authors.forename: Oskar
authors.initials: O
authors.lastname: Adolfsson
authors.forename: Cheol-Koo
authors.initials: CK
authors.lastname: Lee
authors.forename: Tomas A
authors.initials: TA
authors.lastname: Prolla
authors.forename: Jose
authors.initials: J
authors.lastname: Ordovas
authors.forename: Simin Nikbin
authors.initials: SN
authors.lastname: Meydani
mesh_terms: D000375:Aging; D000818:Animals; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007109:Immunity; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D014810:Vitamin E / Q000502:physiology*
chemical_list: D014810:Vitamin E