Is angiotensin II made inside or outside of the cell?
Angiotensin synthesis at tissue sites is well-established, and depends largely, if not completely, on kidney-derived renin. The exact tissue site of angiotensin generation (extracellular fluid, cell surface, intracellular compartment) is still being debated. In this review, we discuss the various possibilities, taking into consideration the intracellular occurrence/absence of prorenin, renin, angiotensinogen, angiotensin-converting enzyme, and angiotensin receptors; the local activation of prorenin to renin; the differences between in vivo and in vitro studies; and the methodologic difficulties related to angiotensin measurements. It is eventually concluded that angiotensin generation at tissue sites occurs extracellularly, most likely on the cell surface.
Chai
Wenxia
W
Department of Pharmacology, Room EE1418b, Erasmus MC, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.
Danser
A H Jan
AH
D000804:Angiotensin II / Q000096:biosynthesis*; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D005110:Extracellular Space / Q000378:metabolism*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D012084:Renin-Angiotensin System / Q000502:physiology*
D000804:Angiotensin II