title: Scanning electrochemical microscopy (SECM) studies of oxygen transfer across phospholipid monolayers under surface pressure control: comparison of monolayers at air/water and oil/water interfaces.
abstract: Scanning electrochemical microscopy has been used in combination with a specially designed Langmuir trough to compare the kinetics of oxygen transfer across an L-alpha-phosphatidylethanolamine, distearoyl monolayer spread at three different interfaces: air/water, air/water in contact with an oil lens, and oil/water. The monolayer is shown to reduce the kinetics of interfacial transport, and rate constants for the transport of oxygen across each interface, at different surface pressures, have been evaluated. The results obtained for each interface are compared, and the implications for studies of passive diffusion across cell membranes are discussed.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.
authors.forename: Susan
authors.initials: S
authors.lastname: Cannan
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Frank
authors.initials: F
authors.lastname: Grunfeld
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Unwin
mesh_terms: D000388:Air; D002462:Cell Membrane; D004058:Diffusion; D004563:Electrochemistry / Q000379:methods; D007700:Kinetics; D020527:Microscopy, Scanning Probe / Q000379:methods; D009821:Oils / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013500:Surface Tension; D014867:Water / Q000737:chemistry*
chemical_list: D009821:Oils; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D014867:Water; C038089:1,2-distearoylphosphatidylethanolamine; D010100:Oxygen