Molecular and electrophysiological characterization of nucleotide-sensitive chloride current-inducing protein of Fasciola hepatica.
Nucleotide-sensitive chloride current regulating proteins (ICln's) of the chloride channels have been characterized from man and animals. An ICln of Fasciola hepatica (ICln-Fh) consisting of 231 amino acids revealed high similarities to both consensus domain of ICln's and two acidic residue-abundant patches in its C-terminus. Native ICln-Fh protein was confirmed present in F. hepatica soluble extract by immunoblotting. The recombinant ICln-Fh protein expressed in collagenase-defolliculated Xenopus oocytes induced fast rising and outward rectifying Cl- currents (I(Cln-Fh)). The recombinant ICln-Fh protein, however, did not trigger cell swelling-induced Cl- currents (I(Cl-swell)). The I(Cln-Fh) currents were significantly reduced by substituting external Cl- with gluconic acid and by externally adding cAMP. Collectively, these results suggest that ICln-Fh protein is an inducer of Cl- currents in F. hepatica.
Park
Jin Bong
JB
Department of Physiology, College of Medicine, Chungnam National University, Taejeon 301-131, Republic of Korea.
Son
Sook Jin
SJ
Lee
Gyu Seung
GS
Cho
Pyo Yun
PY
Song
Ky Sun
KS
Ryu
Pan Dong
PD
Kang
Shin-Yong
SY
Hong
Sung-Jong
SJ
D000595:Amino Acid Sequence; D000818:Animals; D048429:Cell Size; D002712:Chlorides / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D016054:DNA, Protozoan / Q000096:biosynthesis; D004594:Electrophysiology; D005210:Fasciola hepatica / Q000502:physiology*; D007473:Ion Channels / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D014981:Xenopus
D002712:Chlorides; D018076:DNA, Complementary; D016054:DNA, Protozoan; C502022:ICln4 protein, Fasciola hepatica; D007473:Ion Channels; D011994:Recombinant Proteins