title: [Study on the differentially expressed genes in bone tissue of ovariectomized rats after intervention with Chinese herbs].
abstract: OBJECTIVE
  To explore the therapeutic effect and mechanism of jiangu granule (JGG) on postmenopausal osteoporosis.
  
  
  METHODS
  Differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.
  
  
  RESULTS
  Ten differentially expressed gene fragments were found, 9 of them, after cloning, sequencing and BLAST searching, were proved to be matched with highly homologous sequences in Genebank. Among them, two were known proteins: hyaluronan-mediated motility receptor (RHAMM) and ATPase, Na+, K+ transporting beta 3 polypeptide (ATP1b3). It was confirmed by semi-quantitative RT-PCR that these two gene fragments could be down- and up-regulated by JGG respectively.
  
  
  CONCLUSIONS
  The therapeutic effect of JGG in treating postmenopausal osteoporosis might be related with its regulation on the two kinds of protein, RHAMM and ATP1b3.
authors.affiliation: Institute of Integrated Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040.
authors.forename: Yu-ying
authors.initials: YY
authors.lastname: Ma
authors.forename: Xin-min
authors.initials: XM
authors.lastname: Zhang
authors.forename: Shi-feng
authors.initials: SF
authors.lastname: Li
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Gu
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Chen
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D010024:Osteoporosis / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D010052:Ovariectomy; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal