Nonomuraea kuesteri sp. nov.
A Gram-positive, aerobic, non-motile actinomycete, strain GW 14-1925(T), that formed branched substrate and aerial mycelium was studied using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain GW 14-1925(T) was shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea longicatena (97.9 %), Nonomuraea turkmeniaca (98.9 %), Nonomuraea helvata (98.6 %), Nonomuraea polychroma (98.5 %), Nonomuraea salmonaea (98.3 %), Nonomuraea roseoviolacea subsp. roseoviolacea (98.1 %) and Nonomuraea roseoviolacea subsp. carminata (97.7 %). The 16S rRNA gene sequence similarity to other Nonomuraea species was <97.5 %. Chemotaxonomic data [major menaquinones of the MK-9 series with minor amounts of MK-8(H(4)); major polar lipids of phospholipid type IV; fatty acids with major amounts of iso- and anteiso- and 10-methyl-branched fatty acids in combination with iso-branched 2-hydroxy fatty acids] supported allocation of the strain to the genus Nonomuraea. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW 14-1925(T) from closely related species; thus, GW 14-1925(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea kuesteri sp. nov. is proposed, with GW 14-1925(T) (=DSM 44753(T)=NRRL B-24325(T)) as the type strain.
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Kroppenstedt
Reiner M
RM
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-38124 Braunschweig, Germany.
Grün-Wollny
Iris
I
Labor Grün-Wollny, D-35394 Giessen, Germany.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D025282:Mycelium / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S