title: Analysis of cell-free fetal DNA in plasma and serum of pregnant women.
abstract: Sixty blood samples from pregnant women during gestational weeks 9-28 were investigated. Cell-free fetal DNA was extracted from maternal plasma or serum to be detected by nested PCR for determination of fetal gender. The SRY gene as a marker for fetal Y chromosome was detected in 34/36 women carrying a male fetus. In 3/24 women carrying female fetuses, the SRY sequence was also detected. Overall, fetal sex was correctly predicted in 91.7% of the cases. Therefore, the new, non-invasive method of prenatal diagnosis of fetal gender for women at risk of producing children with X-linked disorders is reliable, secure, and can substantially reduce invasive prenatal tests.
authors.affiliation: Research Centre for Medical Genetics, Prenatal Diagnosis, Russian Academy of Medical Sciences, Moskvorechje 1, Moscow 115478, Russia. tvz@medgen.ru
authors.forename: T V
authors.initials: TV
authors.lastname: Zolotukhina
authors.forename: N V
authors.initials: NV
authors.lastname: Shilova
authors.forename: E Yu
authors.initials: EY
authors.lastname: Voskoboeva
mesh_terms: D041322:Chromosomes, Human, Y / Q000235:genetics; D004247:DNA / Q000097:blood*; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012732:Sex Determination Analysis
chemical_list: D004247:DNA