title: An indirect quantitative method for the assessment of angiogenesis in human tissues.
abstract: We set up an ELISA that measures the concentration of von Willebrand factor (vWF) in human endometrial tissue and found a significant correlation with the mean vessel counts in vWF- and CD31-immunostained tissue sections. This ELISA allows an objective and quantitative evaluation of the vascular state in the endometrium and could be used as a method to evaluate the angiogenic state in other tissues.
authors.affiliation: Laboratoire d'Endocrinologie de la Reproduction, Centre de Recherche, Hôpital Saint-François d'Assise, Université Laval, Québec, Québec, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Lawson
authors.forename: Catherine
authors.initials: C
authors.lastname: Herrmann-Lavoie
authors.forename: André
authors.initials: A
authors.lastname: Lemay
authors.forename: Walid
authors.initials: W
authors.lastname: Mourad
authors.forename: Ali
authors.initials: A
authors.lastname: Akoum
mesh_terms: D004717:Endometrium / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014841:von Willebrand Factor