Magnesium and microvascular endothelial cells: a role in inflammation and angiogenesis.
Microvascular endothelial cells are protagonists in inflammation and angiogenesis. Since magnesium (Mg) deficiency promotes inflammation and impairs angiogenesis in vivo, we evaluated the effect of different concentrations of the cation on microvascular 1G11 cells. We found that low Mg inhibits endothelial growth and migration, while it increases some inflammatory markers. In particular we show that low Mg stimulates the synthesis of interleukin 1a and 6, of nitric oxide, a mediator of inflammatory responses, and of VCAM, which mediates monocyte/endothelial interactions. On the contrary, high Mg stimulates proliferation and migration and sensitizes microvascular cells to migratory signals, thus inducing crucial events in angiogenesis. Our results demonstrate a direct role of Mg in modulating microvascular functions and provide a molecular explanation to the link among Mg, angiogenesis and inflammation observed in in vivo models.
Bernardini
Daniela
D
University of Milan, Department of Preclinical Sciences-LITA Vialba, Milano, Italy.
Nasulewic
Anna
A
Mazur
Andrzej
A
Maier
Jeanette A M
JA
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007249:Inflammation / Q000503:physiopathology*; D008274:Magnesium / Q000494:pharmacology*; D051379:Mice; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000378:metabolism
D016207:Cytokines; D019001:Nitric Oxide Synthase; D008274:Magnesium