Secondary effects of femoral instrumentation on pulmonary physiology in a standardised sheep model: what is the effect of lung contusion and reaming?
Intramedullary nailing is the treatment of choice for patients with femoral shaft fractures. However, there is an ongoing debate in multiple trauma patients with associated lung contusion when primary or secondary definitive stabilisation of the long bone fracture should be performed, as nailing is thought to play an important role in the pathogenesis of adult respiratory distress syndrome (ARDS). In a standardised sheep model, this study aimed to quantify the development of acute pulmonary endothelial changes, to assess the activation of polymorphonuclear leucocytes (PMNL) and to observe the effects on the coagulation system associated with the reamed nailing procedure. Furthermore, the effect of coexisting lung contusion in an experimental model was evaluated. The animals were randomly assigned to one of four different groups (6 animals/group). In control groups, only a sham operation (thoracotomy) was performed, whereas in study groups, lung contusion was induced prior to femoral stabilisation either by external fixation or reamed femoral nailing. Using bronchoalveolar lavage (BAL) pulmonary permeability changes were quantified and PMNL activation was assessed by chemiluminescence. Additionally PMNL diapedesis and interstitial lung oedema were determined by histological analysis. All animals were sacrificed 4 h after the start of the femoral instrumentation. Without an associated lung injury, instrumentation of the femoral canal with the reamed nailing technique induced a transient increase in pulmonary permeability. In the face of an induced lung contusion, reamed femoral nailing resulted in significant increases in PMNL activation, pulmonary permeability and interstitial lung oedema, compared with external fixation. Without pulmonary contusion, reaming of the femoral canal was associated with a transient increase in pulmonary permeability. This was exacerbated in the presence of lung contusion along with increased PMNL activation. External fixation did not provoke similar changes. The findings of this study support the view that reaming of the femoral canal should be avoided in polytrauma patients with severe chest trauma as it could act as an additional stimulus for adverse outcome. Temporary external fixation appears to be a safe method for fracture stabilisation until inflammatory and coagulatory disturbances after trauma have been normalized.
Hildebrand
Frank
F
Department of Trauma Surgery, Hanover Medical School, Carl-Neuberg-Strasse 1, 30625 Hanover, Germany. Hildebrand.Frank@mh-hannover.de
Giannoudis
Peter
P
van Griensven
Martijn
M
Chawda
Majur
M
Probst
Christian
C
Harms
Oliver
O
Harwood
Paul
P
Otto
Karl
K
Fehr
M
M
Krettek
Christian
C
Pape
Hans-Christoph
HC
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000990:Antithrombin III / Q000032:analysis; D001777:Blood Coagulation / Q000502:physiology; D002199:Capillary Permeability / Q000502:physiology; D003288:Contusions / Q000209:etiology; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000503:physiopathology; D005165:Factor V / Q000032:analysis; D005260:Female; D005264:Femoral Fractures / Q000503:physiopathology / Q000601:surgery*; D005340:Fibrinogen / Q000032:analysis; D005592:Fracture Fixation / Q000295:instrumentation / Q000379:methods*; D005594:Fracture Fixation, Intramedullary / Q000295:instrumentation / Q000379:methods; D055370:Lung Injury; D009504:Neutrophils / Q000276:immunology; D011652:Pulmonary Circulation / Q000502:physiology; D011654:Pulmonary Edema / Q000209:etiology; D011897:Random Allocation; D012756:Sheep
D000906:Antibodies; D000990:Antithrombin III; D005165:Factor V; D005340:Fibrinogen