title: [uPA expression of gastric cancer cell lines and its correlation with peritoneal seeding].
abstract: OBJECTIVE
  To study the correlation between expression of urokinase-type plasminogen activator (uPA) and capability of tumor cell seeding to the peritoneal membrane by different gastric cancer lines.
  
  
  METHODS
  Expression of uPA in 4 human gastric cancer cell lines was examined by semi-quantitative RT-PCR, ELISA and Western blot. uPA activity was determined by an assay kit. After ip inoculation of cancer cells to nude mice, tumors on peritoneal membrane was grossly examined for tumor cell seedings.
  
  
  RESULTS
  SGC7901 was the highest in uPA expression among human gastric cancer cell lines AGS, SGC7901, MKN45, and MKN28. MKN45 had the strongest uPA activity, while AGS was lowest in both uPA expression and activity. Peritoneal seeding tumors of various sizes were observed in mice inoculated with SGC7901 and MKN45 cells. In addition to peritoneal seedings, bloody ascites was present in mice inoculated with MKN28. The MKN45-inoculated mice took the least time to develop tumors and had the shortest surviving period. No peritoneal seeding was seen in mice inoculated with AGS cells.
  
  
  CONCLUSIONS
  Three of 4 human gastric cancer cell lines studied express uPA mRNA and activity, which correlate with their peritoneal seeding potentials.
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: You-Cheng
authors.initials: YC
authors.lastname: Ding
authors.forename: Zheng-Gang
authors.initials: ZG
authors.lastname: Zhu
authors.forename: Bing-Ya
authors.initials: BY
authors.lastname: Liu
authors.forename: Yu-Bao
authors.initials: YB
authors.lastname: Ji
authors.forename: Xue-Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying-Yan
authors.initials: YY
authors.lastname: Yu
authors.forename: Yan-Zhen
authors.initials: YZ
authors.lastname: Lin
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000556:secondary; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009366:Neoplasm Seeding; D010534:Peritoneal Neoplasms / Q000556:secondary*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator