Diagnostic system for rapid and sensitive differential detection of pathogens.
Naturally emerging and deliberately released pathogens demand new detection strategies to allow early recognition and containment. We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences and report its application for detecting 22 respiratory pathogens in clinical samples.
Briese
Thomas
T
Columbia University, New York, NY 10032, USA.
Palacios
Gustavo
G
Kokoris
Mark
M
Jabado
Omar
O
Liu
Zhiqiang
Z
Renwick
Neil
N
Kapoor
Vishal
V
Casas
Inmaculada
I
Pozo
Francisco
F
Limberger
Ron
R
Perez-Brena
Pilar
P
Ju
Jingyue
J
Lipkin
W Ian
WI
D001424:Bacterial Infections / Q000175:diagnosis*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology / Q000821:virology; D006801:Humans; D017817:Nasal Lavage Fluid / Q000382:microbiology / Q000821:virology; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013183:Sputum / Q000382:microbiology / Q000821:virology; D014777:Virus Diseases / Q000175:diagnosis*
D012329:RNA, Bacterial; D012367:RNA, Viral