Soj antagonizes Spo0A activation of transcription in Bacillus subtilis.
The ParA family protein Soj appears to negatively regulate sporulation in Bacillus subtilis by inhibiting transcription from promoters that are activated by phosphorylated Spo0A. We tested in vitro Soj inhibition of Spo0A-independent variants of a promoter that Soj inhibited (PspoIIG). Transcription from the variants was less sensitive to Soj inhibition, suggesting that inhibition of wild-type PspoIIG was linked to transcription activation by Spo0A.
McLeod
Brett N
BN
Department of Microbiology and Immunology, University of British Columbia, 6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada. spie@interchange.ubc.ca.
Spiegelman
George B
GB
D000255:Adenosine Triphosphate / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012097:Repressor Proteins / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
D001426:Bacterial Proteins; D012097:Repressor Proteins; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors; D000255:Adenosine Triphosphate