Analysis of gene expression induced by diethylstilbestrol (DES) in human primitive Mullerian duct cells using microarray.
The Mullerian ducts are strongly influenced by natural estrogen, estradiol (E2) and diethylstilbestrol (DES) in their development. We screened E2 and DES responsive genes using a microarray analysis in human primitive Mullerian duct cell line, EMTOKA cells expressed estrogen receptor (ER) beta. c-myc oncogene and other target genes expression was detected in cells treated by high-dose DES, but ER antagonist ICI 182,780 could not prevent c-myc induction above. Results of our present study suggested the presence of ER independent pathway in oncogenes induction process by high-dose DES treatment in a human primitive Mullerian duct cell line.
Miki
Yasuhiro
Y
Department of Pathology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi Aoba-ku, Sendai, Miyagi 980-8575, Japan. miki@patholo2.med.tohoku.ac.jp
Suzuki
Takashi
T
Tazawa
Chika
C
Ishizuka
Masato
M
Semba
Shuho
S
Gorai
Itsuo
I
Sasano
Hironobu
H
D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D018929:Cell Culture Techniques; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D020869:Gene Expression Profiling; D016259:Genes, myc; D006801:Humans; D009095:Mullerian Ducts / Q000187:drug effects* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014594:Uterine Neoplasms / Q000139:chemically induced / Q000235:genetics; D014625:Vaginal Neoplasms / Q000139:chemically induced / Q000235:genetics
D018931:Antineoplastic Agents, Hormonal; D011960:Receptors, Estrogen; D004958:Estradiol; D004054:Diethylstilbestrol