title: Differential spontaneous killing of human and murine tumour cell lines by leucocyte subpopulations from carp peripheral blood leucocytes.
abstract: Cell populations from carp (Cyprinus carpio L.) peripheral blood leucocytes (PBLs) were examined for nonspecific cytotoxicities. By using monoclonal antibodies (MAbs) against carp thrombocytes (TCL-HB8) and both neutrophils and monocytes (TCL-BE8), PBLs with a density of 1.08 g ml-1 were separated into three fractions: thrombocytes, a mixture of neutrophils and monocytes, and other cells (mainly lymphocytes), and the separated cells were tested for cytotoxic activities against mammalian tumour cell lines (K562, HeLa, P815 and Yac-1 cell). Consequently, the mixture of neutrophils and monocytes exhibited cytolysis against these target cells, whereas the lymphocyte-rich and thrombocyte fractions did not show any cytolysis. To isolate only neutrophils, which do not contain monocytes, the MAb (TCL-BE8) positive cells from PBLs with a density of 1.08-1.09 g ml-1 were separated. Pure isolated neutrophils showed cytotoxic activities against K562 cells, but not P815 cells. Furthermore, analysis of the cytolytic mechanisms indicated that killing of these cells depended on H2O2 or HOCl. These results suggest that both neutrophils and monocytes are effectors for nonspecific cytotoxicity in carp PBLs, and neutrophils may be distinct from monocytes in their reactivity in cytolysis, including target cell selectivity and/or target cell sensitivity, and the cytolytic pathway. In carp, cytotoxicity of target cells can be mediated by several populations of their leucocytes which have cytotoxic capacities with various recognition and cytolytic mechanisms.
authors.affiliation: Inland Station, National Research Institute of Aquaculture, Tamaki, Mie 519-0423, Japan. cnakayas@fra.affrc.go.jp
authors.forename: Chihaya
authors.initials: C
authors.lastname: Nakayasu
authors.forename: Tomonori
authors.initials: T
authors.lastname: Somamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Yoshitomi
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000276:immunology; D002347:Carps / Q000276:immunology*; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000276:immunology; D006997:Hypochlorous Acid / Q000276:immunology; D007962:Leukocytes / Q000276:immunology* / Q000648:ultrastructure; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide