title: Localization of poly-L-lysine-photosensitizer conjugate  in nucleus.
abstract: The cellular uptake and photocytotoxity of poly-l-lysine (pL)-chlorin e6 (Ce6) conjugate were investigated. The cellularuptake of pL-Ce6 conjugate for HeLa cells was much higher than that of Ce6, and pL-Ce6 conjugate had high binding affinityfor HeLa cells. pL-Ce6 conjugate was accumulated in the nucleus of HeLa cells, and the effective photocytotoxity wasobserved by the irradiation.
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Shun-ichiro
authors.initials: S
authors.lastname: Ogura
authors.forename: Keiko
authors.initials: K
authors.lastname: Yazaki
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kamachi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Okura
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002735:Chlorophyllides; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D017319:Photosensitizing Agents / Q000032:analysis / Q000378:metabolism*; D011107:Polylysine / Q000032:analysis / Q000378:metabolism*; D011166:Porphyrins / Q000032:analysis / Q000378:metabolism
chemical_list: D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; D011107:Polylysine; C062985:phytochlorin