title: Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, including confirmation with N. gonorrhoeae-specific 16S rRNA PCR, with traditional culture.
abstract: A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.
authors.affiliation: Regional Public Health Laboratory, van Ketwich Verschuurlaan 92, 9721 SW Groningen, The Netherlands. j.schirm@slgd.nl.
authors.forename: Dirk S
authors.initials: DS
authors.lastname: Luijt
authors.forename: Petra A J
authors.initials: PA
authors.lastname: Bos
authors.forename: Anton A
authors.initials: AA
authors.lastname: van Zwet
authors.forename: Pieter C
authors.initials: PC
authors.lastname: van Voorst Vader
authors.forename: Jurjen
authors.initials: J
authors.lastname: Schirm
mesh_terms: D003470:Culture Media; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D012336:RNA, Ribosomal, 16S