Sp-family of transcription factors regulates human SHIP2 gene expression.
We have characterized the regulation of human SH-2 containing inositol 5'-phosphatase 2 (SHIP2) gene expression. First, the transcription initiation sites and the sequence of the 5' upstream region of human SHIP2 gene were elucidated. Next, the minimal promoter of the human SHIP2 gene was identified by reporter gene assays in HL60 cells and differentiated human subcutaneous white adipocytes. An Sp1 element proximal to the transcription initiation site was indispensable for full promoter activity and bound specifically by Sp1 and Sp3 proteins. These findings suggest that human SHIP2 gene expression, like other housekeeping genes, is controlled by the Sp-family of transcription factors.
Ishida
Satoru
S
Institute for Clinical Research, National Kyushu Cancer Center, Fukuoka 811-1395, Japan.
Funakoshi
Akihiro
A
Miyasaka
Kyoko
K
Iguchi
Haruo
H
Takiguchi
Soichi
S
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D051705:Sp3 Transcription Factor; D014157:Transcription Factors / Q000378:metabolism*; D024363:Transcription Initiation Site; D014162:Transfection
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009841:Oligonucleotides; D011993:Recombinant Fusion Proteins; C497121:SP3 protein, human; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D051705:Sp3 Transcription Factor; D008156:Luciferases; D010744:Phosphoric Monoester Hydrolases; C098706:INPPL1 protein, human; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases