[Isolation and structural analysis of a new polysaccharid, Streptomyces polysaccharide].
OBJECTIVE
To isolate and purify a new polysaccharid Streptomyces polysaccharid polysaccharide (SMP ), from cultured broth of a Streptomyces sp.strain and perform its structural analysis.


METHODS
Ethanol was used to precipitate the polysaccharides and macromolecules from the broth. The proteins in the precipitate were removed by Sevage method and purification of SMP was carried out by DEAE-celluloseion-exchange chromatography and Sephadex G-25 gel filtration. The chemical structure of the SMP was determined by combined application of high performance liquid chromatography (HPLC), UV, IR and 1H-NMR spectroscopy, supplemented by periodate oxidation and Smith degradation.


RESULTS
The purified SMP was neutral by a relative molecular mass of approximately 4 855. Sugar analysis showed that SMP contained glucose and fructose residues in an approximate molar ratio of 22:1 (10.96 to 0.48). The glycosidic linkages were estimated to be (1-6)- alpha-D- pyranoside form.


CONCLUSIONS
SMP is characterized as a (1-6)- alpha-D- pyranose.
Wen
Xiao-Yun
XY
Institute of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Zhu
Zheng-Guang
ZG
Lin
Bi-Run
BR
Xie
Shuang-da
SD
Lei
Lin-Sheng
LS
Wu
Shu-Guang
SG
D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification*; D012987:Soil / Q000032:analysis; D013302:Streptomyces / Q000737:chemistry*
D011135:Polysaccharides, Bacterial; D012987:Soil