title: SEB-induced T cell apoptosis in atopic patients--correlation to clinical status and skin colonization by Staphylococcus aureus.
abstract: BACKGROUND
  We asked whether in atopic dermatitis (AD) increased T cell apoptosis in staphylococcal enterotoxin B (SEB)-activated cultures of peripheral blood mononuclear cells (PBMCs) is characteristic of the exacerbation of the disease or connected with skin colonization by Staphylococcus aureus.
  
  
  METHODS
  The clinical status of the patients was evaluated using the SCORAD index. The number of bacteria colonizing patients' skin lesions was determined by the cfu method. Mononuclear cells isolated from peripheral blood were stimulated by SEB and the apoptosis of CD3+ cells in culture was determined by flow cytometry using the monoclonal antibody APO2.7. The cytokine production in the culture supernatants was determined by ELISA and Cytometric Bead Array kits.
  
  
  RESULTS
  T cell apoptosis was increased, while the production of interferon (IFN)-gamma was reduced in cultures of PBMCs of AD patients during exacerbation. The proportion of CD3+ APO2.7+ cells positively correlated with the density of S. aureus recovered from skin lesions, but not with SCORAD index. By contrast, SCORAD index, but not S. aureus density, negatively correlated with IFN- gamma production. Furthermore it was found that the presence of S. aureus on uninvolved skin distinguishes a group of severe cases with high serum IgE level, increased T cell apoptosis, and reduced production of tumor necrosis factor alpha in SEB- -stimulated cultures.
  
  
  CONCLUSIONS
  Among AD patients the increased activation-induced T cell apoptosis observed in SEB- -stimulated cultures is related to skin colonization by S. aureus. The presence of bacteria on uninvolved skin is a feature of a distinct group of AD patients.
authors.affiliation: Department of Microbiology, Institute of Pediatrics, Cracow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kedzierska
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kaszuba-Zwoińska
authors.forename: Zofia
authors.initials: Z
authors.lastname: Słodowska-Hajduk
authors.forename: Monika
authors.initials: M
authors.lastname: Kapińska-Mrowiecka
authors.forename: Marzena
authors.initials: M
authors.lastname: Czubak
authors.forename: Piotr
authors.initials: P
authors.lastname: Thor
authors.forename: Kinga
authors.initials: K
authors.lastname: Wójcik
authors.forename: Juliusz
authors.initials: J
authors.lastname: Pryjma
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis; D003876:Dermatitis, Atopic / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D004768:Enterotoxins / Q000633:toxicity*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D012867:Skin / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000472:pathogenicity*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology*
chemical_list: D016207:Cytokines; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal