[The inhibitory effect of deoxynivalenol on TAP-1 expression in human peripheral blood mononuclear cells in vitro].
OBJECTIVE
To explore the effects of deoxynivalenol (DON) on transporter associated with antigen processing-1 (TAP-1) expression in human peripheral blood mononuclear cells (PBMCs).


METHODS
Effects of various concentrations of DON on TAP-1 expression of in vitro cultured human PBMCs and the dose-effect relationship were analyzed by flow cytometry (FCM) and semi-quantitative RT-PCR at the protein and mRNA levels.


RESULTS
FCM analysis indicated that treatment with various concentrations of DON could inhibit TAP-1 expressions in PBMCs, showing a negative correlation between DON concentration and TAP-1 expression. Semi-quantitative RT-PCR detection indicated that high concentrations of DON (1,000 and 2,000 microg/L) could distinctly inhibit TAP-1 mRNA expression.


CONCLUSIONS
DON can down-regulate TAP-1 expression in human PBMCs in a dose-dependent manner in vitro, which suggests DON may interfere with host immunosurveillance, and this may account for the correlation between DON-contaminated grain and esophagus cancer in high risk area.
Li
Yue-hong
YH
Lab.of Experiment Pathology, Hebei Medical University, Shijiazhuang 050017, China.
Zhang
Xiang-hong
XH
Xing
Ling-xiao
LX
Yan
Xia
X
Wang
Jun-ling
JL
Wang
Feng-rong
FR
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000328:Adult; D004305:Dose-Response Relationship, Drug; D004938:Esophageal Neoplasms / Q000139:chemically induced; D005434:Flow Cytometry; D005506:Food Contamination; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012306:Risk; D014255:Trichothecenes / Q000633:toxicity*
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D012333:RNA, Messenger; C073119:TAP1 protein, human; D014255:Trichothecenes; C007262:deoxynivalenol