[Effects of selenium on benzo[a] pyrene-induce DNA damage in mouse lung cells].
OBJECTIVE
To explore the effects of selenium on DNA damage induced by benzo[a] pyrene (BaP) in mouse lung cells.


METHODS
Sodium selenite was given to Kunming male mice by i.p. and BaP was given by oral gavage. The control group was given solvent only with the same method. DNA damage was detected by single cell gel electrophoresis (or comet assay).


RESULTS
The damage degrees in mice treated with 125, 250 and 500 mg/kg of BaP were more severe than that of control (P < 0.01). The rates of comet cells in the BaP-treated groups (43.50%, 84.00%, 95.63%) were significantly higher than that of control (9.75%, P < 0.01), and there was obvious dose-response relationship. 0.75, 1.50 and 3.00 mg/kg of sodium selenite presented antagonistic effects against DNA damage induced by 250 mg/kg of BaP in mouse's lung cells. The antagonistic effect of sodium selenite at the dose of 1.50 mg/kg was better than those of sodium selenite at the doses of 0.75, 3.00 mg/kg.


CONCLUSIONS
BaP at the doses of 125 approximately 500 mg/kg could significantly induce DNA damage of lung cells in mice. 0.75 approximately 3.00 mg/kg of sodium selenite could inhibit DNA damage of lung cells in mice induced by 250 mg/kg of BaP.
Yu
Ri-An
RA
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Li
Xiao-Yan
XY
Lu
Wen-Qing
WQ
Mei
Yun-Hua
YH
Zhu
Jian-Lin
JL
Chen
Xiu-Na
XN
Chen
Xue-Min
XM
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012643:Selenium / Q000494:pharmacology*
D001564:Benzo(a)pyrene; D012643:Selenium