CD8alpha-11b+ dendritic cells but not CD8alpha+ dendritic cells mediate cross-tolerance toward intestinal antigens.
Cross-presentation is a critical process by which antigen is displayed to CD8 T cells to induce tolerance. It is believed that CD8alpha+ dendritic cells (DCs) are responsible for cross-presentation, suggesting that the CD8alpha+ DC population is capable of inducing both cross-priming and cross-tolerance to antigen. We found that cross-tolerance against intestinal soluble antigen was abrogated in C57BL/6 mice lacking mesenteric lymph nodes (MLNs) and Peyer patches (PPs), whereas mice lacking PPs alone were capable of developing CD8 T-cell tolerance. CD8alpha-CD11b+ DCs but not CD8alpha+ DCs in the MLNs present intestinal antigens to relevant CD8 T cells, while CD8alpha+ DCs but not CD8alpha-CD11b+ DCs in the spleen exclusively cross-present intravenous soluble antigen. Thus, CD8alpha-CD11b+ DCs in the MLNs play a critical role for induction of cross-tolerance to dietary proteins.
Chung
Yeonseok
Y
Laboratory of Immunology, Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Shillimdong, Kwanak-Gu, Seoul 151-742, Korea.
Chang
Jae-Hoon
JH
Kweon
Mi-Na
MN
Rennert
Paul D
PD
Kang
Chang-Yuil
CY
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D039481:CD11b Antigen / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004044:Dietary Proteins / Q000276:immunology; D004750:Enteral Nutrition; D005260:Female; D007108:Immune Tolerance / Q000276:immunology*; D007275:Injections, Intravenous; D007422:Intestines / Q000166:cytology / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D010581:Peyer's Patches / Q000276:immunology / Q000473:pathology; D012995:Solubility
D039481:CD11b Antigen; D016827:CD8 Antigens; D004044:Dietary Proteins; D010047:Ovalbumin