title: Dynamic response of plant genome to ultraviolet radiation and other genotoxic stresses.
abstract: Oligonucleotide microarray technology was used to identify genes, which are responding after exposure to UV-C radiation and to other agents causing genotoxic stress. The effect of these conditions on recombinational DNA repair was monitored in parallel. Global changes in gene expression were investigated in Arabidopsis wild-type plants challenged with UV-C, bleomycin, another abiotic agent and xylanase, a biotic factor, all leading to elevated homologous recombination frequencies. The comparison of the expression profile of each treatment allowed defining genes specifically involved in the dynamic response to UV. In the future, the potential roles of such genes in the different forms of stress recognition, signal transduction, and their roles in DNA repair processes will be assessed by using reverse genetic tools available for Arabidopsis thaliana.
authors.affiliation: Friedrich Miescher-Institute for Biomedical Research, Maulbeerstrasse 66, Basel CH-4058, Switzerland. jemolini@univ-bpclermont.fr
authors.forename: Jean
authors.initials: J
authors.lastname: Molinier
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Oakeley
authors.forename: Olivier
authors.initials: O
authors.lastname: Niederhauser
authors.forename: Igor
authors.initials: I
authors.lastname: Kovalchuk
authors.forename: Barbara
authors.initials: B
authors.lastname: Hohn
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D001761:Bleomycin / Q000633:toxicity; D043364:Endo-1,4-beta Xylanases / Q000633:toxicity; D020869:Gene Expression Profiling; D018745:Genome, Plant; D009153:Mutagens / Q000633:toxicity*; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
chemical_list: D009153:Mutagens; D001761:Bleomycin; D043364:Endo-1,4-beta Xylanases