A tetracycline-regulatable adeno-associated virus vector for double-gene transfer.
An increasing demand for polycistronic vectors that express multiple genes simultaneously has arisen in recent years to obtain an efficient gene therapy. Armed with the knowledge that the expression of transgene in mammalian cells often requires tight control, we constructed in this study a tetracycline-regulated double-gene adeno-associated virus (AAV) vector carrying green and red fluorescent protein genes and expressed it in PC12 cells. When detected by fluorescence microscope and fluorescence-activated cell sorting, gene expression was induced by 44-66-fold and could be reversibly controlled by doxycycline. This double-gene AAV vector may be useful for regulated expression of two genes or a marker to monitor transgene expression.
Wang
Jian-jun
JJ
Neuroscience Research Institute, Peking University, Beijing 100083, PR China.
Niu
Dong-bin
DB
Zhang
Ting
T
Wang
Kun
K
Xue
Bing
B
Wang
Xiao-min
XM
D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D013752:Tetracycline / Q000494:pharmacology*; D014162:Transfection / Q000379:methods; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D013752:Tetracycline; D000097573:Red Fluorescent Protein