Purifying the masses: integrating prepurification quality control, high-throughput LC/MS purification, and compound plating to feed high-throughput screening.
In this paper we report using a parallel, four-channel HPLC/MUX/MS purification system, the Purification Factory, to purify thousands of compounds destined for high-throughput screening in a single month. The maximum sample throughput during this 20-workday month was 704 samples/day. Since this purification throughput exceeded the postpurification sample and data handling capabilities provided by commercial solutions, a custom-integrated solution was designed to address these shortcomings. In this paper we detail the key improvements in automation, solvent handling, and sample handling logistics implemented to sustain a mean throughput of 528 samples/day over a multimonth time period.
Isbell
John J
JJ
Genomics Institute of the Novartis Research Foundation (GNF), 10675 John Jay Hopkins Drive, San Diego, California 92121, USA.
Zhou
Yingyao
Y
Guintu
Christina
C
Rynd
Matthew
M
Jiang
Shumei
S
Petrov
Dimitri
D
Micklash
Ken
K
Mainquist
Jim
J
Ek
Jared
J
Chang
Jim
J
Weselak
Mark
M
Backes
Bradley J
BJ
Brailsford
Andrew
A
Shave
Darcy
D
D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000302:isolation & purification* / Q000592:standards; D011786:Quality Control; D012371:Robotics
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations