title: Hydrogen sulphide is a mediator of carrageenan-induced hindpaw oedema in the rat.
abstract: Hydrogen sulphide (H(2)S) is a naturally occurring gas, with potent vasodilator activity. In this report, we identify a role for H(2)S in carrageenan-induced hindpaw oedema in the rat. Intraplantar injection of carrageenan (150 microl, 2% (w v(-1))) resulted in an increase in hindpaw H(2)S synthesising enzyme activity and increased myeloperoxidase (MPO) activity. Pretreatment (i.p. 60 min before carrageenan) with DL-propargylglycine (PAG, 25-75 mg kg(-1)), an inhibitor of the H(2)S synthesising enzyme cystathionine-gamma-lyase (CSE), significantly reduced carrageenan-induced hindpaw oedema in a dose-dependent manner (e.g. increase in hindpaw weight at 3 h, saline: 0.12+/-0.017 g; carrageenan, 1.39+/-0.037 g; PAG, 50 mg kg(-1), 1.11+/-0.06 g, n=10) and MPO activity (fold increase) in the hindpaw (saline: 1.0+/-0.12; carrageenan, 2.92+/-0.45 g; PAG, 50 mg kg(-1), 1.1+/-0.22, n=10); PAG (50 mg kg(-1)) also inhibited H(2)S synthesising enzyme activity (nmol microg DNA(-1)) in the hindpaw in a dose-dependent manner (saline, 0.46+/-0.05; carrageenan, 0.71+/-0.08 g; PAG, 50 mg kg(-1), 0.17+/-0.05, n=10).
authors.affiliation: Department of Pharmacology, Cardiovascular Biology Research Group, National University of Singapore, 18 Medical Drive, Singapore 117597, Singapore. mbhatia@nus.edu.sg
authors.forename: Madhav
authors.initials: M
authors.lastname: Bhatia
authors.forename: Jenab
authors.initials: J
authors.lastname: Sidhapuriwala
authors.forename: Shabbir M
authors.initials: SM
authors.lastname: Moochhala
authors.forename: Philip K
authors.initials: PK
authors.lastname: Moore
mesh_terms: D000480:Alkynes / Q000494:pharmacology; D000818:Animals; D002351:Carrageenan; D003542:Cystathionine gamma-Lyase / Q000037:antagonists & inhibitors; D004487:Edema / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006614:Hindlimb; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D009195:Peroxidase / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000480:Alkynes; C009055:propargylglycine; D002351:Carrageenan; D009195:Peroxidase; D003542:Cystathionine gamma-Lyase; D005998:Glycine; D006862:Hydrogen Sulfide