An interference-free implantable glucose microbiosensor based on use of a polymeric analyte regulating membrane.
An implantable glucose microbiosensor that utilizes an electrodeposited redox polymer/glucose oxidase (GOX) sensing membrane and a highly hydrophilic poly (4-vinyl pyridine-co-acrylic acid) (PVP-PAC) overcoating as an analyte-regulating membrane has been developed. A gold microelectrode was electrodeposited with the redox polymer/GOX composite sensing membrane. Two polymers, a hydrophobic poly (4-vinyl pyridine) (PVP) and a hydrophilic PVP-PAC were investigated as possible analyte-regulating membranes. Each was mounted on the sensing layer via chemical cross-linking with poly(ethylene glycol) diglycidyl ether (PEGDGE). High current sensitivities of approximately 220 nA/mM glucose were obtained in the presence of PVP-PAC. In the case of PVP, much lower sensitivities of approximately 30 nA/mM were observed. However, only the biosensor with PVP-PAC showed excellent selectivity to glucose against interferants like oxygen and ascorbic acid (AA). The dynamic range is from 0 to 30 mM. The response time in amperometric measurements was less than 10 sec. Clinical trials showed good correlation between the readings of the subcutaneously implanted biosensor and blood sugar levels.
Xie
Hong
H
Institute of Bioengineering and Nanotechnology, Biopolis Way, Singapore 138669.
Tan
Xiu Ling
XL
Gao
Zhiqiang
Z
D015374:Biosensing Techniques / Q000379:methods; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D011108:Polymers; D005949:Glucose Oxidase; D005947:Glucose