title: Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
abstract: Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.
authors.affiliation: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, People's Republic of China.
authors.forename: Maofeng
authors.initials: M
authors.lastname: Qiu
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.forename: Zeliang
authors.initials: Z
authors.lastname: Chen
authors.forename: Erhei
authors.initials: E
authors.lastname: Dai
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Pang
authors.forename: Baoxing
authors.initials: B
authors.lastname: Fan
authors.forename: Jie
authors.initials: J
authors.lastname: Wen
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis; D001665:Binding Sites; D017934:Coronavirus / Q000737:chemistry / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D006801:Humans; D019251:Nucleocapsid / Q000032:analysis*; D012128:Respiratory Distress Syndrome / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D000914:Antibodies, Viral