Pilot study of a breath test for volatile organic compounds associated with oral malodor: evidence for the role of oxidative stress.
BACKGROUND
We performed a pilot study of a new method to identify the volatile organic compounds (VOCs) in breath associated with oral malodor, using gas chromatography and mass spectroscopy (GC/MS).


METHODS
Oral cavity breath was collected from seven patients with oral malodor. Breath samples (150 ml) were concentrated onto sorbent traps and analyzed by GC/MS.


RESULTS
Organoleptic scores ranged from 3.0 to 4.0 (mean = 3.3) on a scale of 0-5. Twenty-four of 30 (80.0%) of the most abundant oral malodor volatile organic compounds (OMVOCs) were alkanes and methylated alkanes. These VOCs are products of oxidative stress, generated by lipid peroxidation of polyunsaturated fatty acids in cell membranes.


CONCLUSIONS
Increased oxidative stress in the oral cavity of patients with oral malodor may account for the increased risk of atherosclerosis, coronary heart disease and stroke associated with periodontal disease. The breath test for OMVOCs could potentially provide an objective new test for the assessment of oral malodor.
Phillips
M
M
Menssana Research Inc., Fort Lee, NJ 07024, USA. mphillips@menssanaresearch.com
Cataneo
R N
RN
Greenberg
J
J
Munawar
Mi
M
Nachnani
S
S
Samtani
S
S
D000473:Alkanes / Q000032:analysis*; D001944:Breath Tests / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006209:Halitosis / Q000175:diagnosis* / Q000378:metabolism*; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010865:Pilot Projects
D000473:Alkanes