Elicitation of diosgenin production in Dioscorea floribunda by ethylene-generating agent.
Dioscorea floribunda cells aggregates were cultured in liquid modified MS medium supplemented with 2,4-D (2 mg/l) and kinetine (0.1 mg/l). The cells were treated with different concentrations of ethylene-generating-agent 2-chloroethylphosphonic acid (2-CEPA). 2-CEPA at concentrations of 50 mg/l, 100 mg/l elicited production of diosgenin. The level of diosgenin increased to 72-fold higher than those of non-treated cells with 100 mg/l of 2-CEPA.
De
Debjani
D
Pharmacognosy Research Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Calcutta 700019, India.
De
Bratati
B
D046508:Culture Techniques; D027742:Dioscorea; D004144:Diosgenin / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism; D006801:Humans; D009943:Organophosphorus Compounds / Q000494:pharmacology; D008517:Phytotherapy; D010937:Plant Growth Regulators / Q000494:pharmacology
D005030:Ethylenes; D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; C036216:ethylene; D004144:Diosgenin; C005073:ethephon