Does activated protein C-resistant factor V contribute to thrombin generation in hemophilic plasma?
In this study we assessed the role of factor V (FV) inactivation in hemophilic plasma with particular reference to the activated protein C (APC)-resistant variants FV-R506Q (FV Leiden) and FV-R306T (FV Cambridge). Purified recombinant full-length FV carrying these single substitutions and FV-R306T/R506Q were used in thrombin generation experiments. Plasma was first immunodepleted of FV, and subsequently of factors VIII, IX, or combinations thereof. Thrombin generation was initiated by low concentrations of recombinant tissue factor. Recombinant soluble thrombomodulin (TM) was used to trigger the APC system. Surprisingly, TM concentrations that reduced thrombin generation in normal plasma by no more than 50% virtually abolished thrombin formation in plasma deficient in the factor VIII/IX complex. This was already apparent at TM levels as low as 0.1 nmol L(-1). By varying the concentrations of purified (activated) protein C to plasma that was additionally depleted of protein C, we confirmed that impaired thrombin generation indeed was the result of the action of APC. In contrast, this did not occur when FV-depleted plasma had been reconstituted with FV-R306T/R506Q. Addition of FV-R306T or FV-R506Q partially reduced prothrombin activation, demonstrating the involvement of both APC cleavage sites. FV inactivation also occurred on the surface of human microvascular endothelial cells. Apparently, these cells express sufficient TM to down-regulate thrombin production via the APC pathway. We further conclude that in hemophilic plasma this pathway can induce a secondary defect because of premature FV inactivation. It therefore seems conceivable that APC-resistant FV has the potential of alleviating hemophilic bleeding.
Bos
M H A
MH
The Department of Plasma Proteins, Sanquin Research at CLB, Amsterdam, the Netherlands.
Meijerman
D W E
DW
van der Zwaan
C
C
Mertens
K
K
D020016:Activated Protein C Resistance; D019943:Amino Acid Substitution; D001777:Blood Coagulation; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000473:pathology; D005165:Factor V / Q000235:genetics*; D006467:Hemophilia A / Q000097:blood / Q000209:etiology* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D010949:Plasma / Q000378:metabolism / Q000502:physiology; D013917:Thrombin / Q000096:biosynthesis*; D018180:Thrombomodulin
D018180:Thrombomodulin; D005165:Factor V; D013917:Thrombin