title: Ploidy analysis of field cancerization and cancer development in the hamster cheek pouch carcinogenesis model.
abstract: BACKGROUND
  The hamster cheek-pouch carcinogenesis model is a well-known animal system that closely mimics the development of premalignant and malignant lesions in human oral cancer. Our aim was to numerically characterize the premalignant and malignant lesions and expressions of field cancerization in this model using ploidy as the end-point.
  
  
  METHODS
  To study the DNA content and proliferation status of the cells in this model we assessed the Feulgen reaction and the immunohistochemical reaction for 5-bromo-2-deoxiuridine (BrdU) in different histological areas of serial tissue sections of the cheek pouches of animals injected with BrdU.
  
  
  RESULTS
  Ploidy values were higher in cancerized epithelia with no unusual microscopic features (NUMF), in preneoplastic and tumor areas than in control epithelia. The aneuploidy index was higher in NUMF areas than in control and differed significantly from control in preneoplastic areas and carcinoma.
  
  
  CONCLUSIONS
  The unexpected alteration in DNA content observed in NUMF epithelia is of great relevance as a biomarker of field cancerized areas.
authors.affiliation: Oral Pathology Department, Faculty of Dentistry, University of Buenos Aires, 1122 Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Raimondi
authors.forename: R
authors.initials: R
authors.lastname: Cabrini
authors.forename: M E
authors.initials: ME
authors.lastname: Itoiz
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects; D000782:Aneuploidy; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002273:Carcinogens / Q000009:adverse effects; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D002278:Carcinoma in Situ / Q000235:genetics / Q000473:pathology; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000032:analysis; D004195:Disease Models, Animal; D004848:Epithelium / Q000473:pathology; D005260:Female; D006965:Hyperplasia; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000473:pathology*; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D011003:Ploidies; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D002273:Carcinogens; D004273:DNA, Neoplasm; D015127:9,10-Dimethyl-1,2-benzanthracene