title: TILLING without a plough: a new method with applications for reverse genetics.
abstract: TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful reverse genetic technique that employs a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms. Its advantages over other reverse genetic techniques include its applicability to virtually any organism, its facility for high-throughput and its independence of genome size, reproductive system or generation time. TILLING is currently being used for the detection of both induced and natural variation in several plant species.
authors.affiliation: Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, British Colombia, V6T 1Z4, Canada.
authors.forename: Erin J
authors.initials: EJ
authors.lastname: Gilchrist
authors.forename: George W
authors.initials: GW
authors.lastname: Haughn
mesh_terms: D000383:Agriculture / Q000379:methods / Q000639:trends; D020029:Base Pairing; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D016297:Mutagenesis, Site-Directed; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D018744:DNA, Plant