title: Automated preparation of hypoxic cell marker [18F]FRP-170 by on-column hydrolysis.
abstract: An automated synthesis for the preparation of the novel hypoxic cell marker, [(18)F]FRP-170 3, [(18)F]1-[2-fluoro-1-(hydroxymethyl)ethoxy]methyl-2- nitroimidazole, was developed using an on-column basic-hydrolysis step. The (18)F-labeled protected intermediate 2 was retained on a Sep-Pak Plus C18 cartridge and, in the same cartridge at room temperature, hydrolyzed by NaOH for deacetylation to give [(18)F]FRP-170. The elution method from the cartridge was optimized for direct injection of the crude product into an HPLC column. Thus, [(18)F]FRP-170 was prepared in 20-30% decay-corrected radiochemical yield within 60 min.
authors.affiliation: CYRIC Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: R
authors.initials: R
authors.lastname: Iwata
authors.forename: S
authors.initials: S
authors.lastname: Furumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
mesh_terms: D001331:Automation; D015415:Biomarkers; D015687:Cell Hypoxia; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D006868:Hydrolysis
chemical_list: D015415:Biomarkers; D005462:Fluorine Radioisotopes