title: Surface triggering receptor expressed on myeloid cells 1 expression patterns in septic shock.
abstract: OBJECTIVE
  To analyze the pattern of cell-surface expression of the triggering receptor expressed on myeloid cells (TREM) 1 during septic shock.
  
  
  METHODS
  Prospective clinical study in an adult 16-bed medical ICU.
  
  
  METHODS
  25 septic shock patients, 15 patients with shock of noninfectious origin and 7 healthy volunteers. Arterial blood was drawn within 12 h of admission and subjected to flow cytometry analysis after staining with anti-TREM-1 and anti-CD14 antibodies. Repeated sampling was performed on days 2, 3, 5, 7, and 14 in septic shock patients.
  
  
  RESULTS
  Monocytic TREM-1 expression was significantly higher in septic shock patients (mean fluorescence intensity 2.3+/-0.2) than in nonseptic patients (1.0+/-0.1), and healthy volunteers (1.0+/-0.1). There was no difference in monocytic TREM-1 expression between nonseptic patients and healthy volunteers or between any of the three groups with respect to TREM-1 expression on neutrophils. The time course of TREM-1 expression on monocytes diverged significantly by day 3 between survivors and ns.
  
  
  CONCLUSIONS
  The specificity of TREM-1 regulation by infection is highlighted. Moreover, surface TREM-1 expression on monocytes may prove useful in allowing the follow-up of septic patients during the course of the disease.
authors.affiliation: Réanimation Médicale, Hôpital Central, 54000 Nancy, France. s.gibot@chu-nancy.fr
authors.forename: Sébastien
authors.initials: S
authors.lastname: Gibot
authors.forename: Pol-Edern
authors.initials: PE
authors.lastname: Le Renard
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Bollaert
authors.forename: Marie-Nathalie
authors.initials: MN
authors.lastname: Kolopp-Sarda
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Béné
authors.forename: Gilbert C
authors.initials: GC
authors.lastname: Faure
authors.forename: Bruno
authors.initials: B
authors.lastname: Lévy
mesh_terms: D000368:Aged; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007362:Intensive Care Units; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology*; D011446:Prospective Studies; D011971:Receptors, Immunologic / Q000276:immunology*; D012772:Shock, Septic / Q000276:immunology*; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
chemical_list: D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1