Epidemiology of human sporotrichosis investigated by amplified fragment length polymorphism.
Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of Peruvian strains of Sporothrix schenckii and to compare them to a panel of non-Peruvian strains. AFLP analysis suggests that the Peruvian strains can be divided into two homogeneous clusters with no reference to geographical origin or the clinical form of sporotrichosis. The strains from abroad present heterogeneous profiles, with the Bolivian strain and the Colombian strains related to one of the Peruvian population. Sequencing of internal transcribed spacer 2, used to examine the relationships over a longer distance, confirmed the division of Peruvian strains into two populations that can be identified on the basis of a single but specific sequence divergence. This paper introduces automated AFLP analysis as a valuable tool for further investigation of the epidemiology and ecology of S. schenckii.
Neyra
Edgar
E
Scientific Institute of Public Health, Mycology Section, Rue J. Wytsman 14, 1050 Brussels, Belgium.
Fonteyne
Pierre-Alain
PA
Swinne
Danielle
D
Fauche
Frederic
F
Bustamante
Beatriz
B
Nolard
Nicole
N
D001483:Base Sequence; D004463:Ecology; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013175:Sporothrix / Q000235:genetics*; D013174:Sporotrichosis / Q000453:epidemiology*