title: Bovine BMP osteoinductive potential enhanced by functionalized dextran-derived hydrogels.
abstract: This study evaluated functionalized dextran-derived hydrogels as BMP carriers using both in vitro and in vivo models. In vitro release kinetics indicated that dextran-derived hydrogels could retain rhBMP-2 growth factor in a variable manner depending on their functionalization ratio. The potential of these hydrogels when combined with extracted bovine BMP to enhance the bone formation was evaluated in a rat ectopic model. The largest osteoinduction was found when using hydrogels exhibiting the highest growth factor retention capacity. In addition, some implanted hydrogels demonstrated a capacity to induce an in-vivo calcification certainly related to their chemical composition. These properties make these materials interesting osteoconductive BMP carriers, allowing to decrease the amount of implanted factor required for bone regeneration.
authors.affiliation: Laboratoire de Recherches Orthopédiques, UMR 7052 CNRS-SPI, Faculté de Médecine Lariboisière-Saint-Louis, Université Paris 7, 10 Avenue de Verdun, 75010 Paris, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Maire
authors.forename: Frederic
authors.initials: F
authors.lastname: Chaubet
authors.forename: Pierrer
authors.initials: P
authors.lastname: Mary
authors.forename: Cinderella
authors.initials: C
authors.lastname: Blanchat
authors.forename: Alain
authors.initials: A
authors.lastname: Meunier
authors.forename: Delphine
authors.initials: D
authors.lastname: Logeart-Avramoglou
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage* / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D004343:Drug Implants / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000737:chemistry
chemical_list: C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D003911:Dextrans; D004343:Drug Implants; D020100:Hydrogels; D016212:Transforming Growth Factor beta