Gelatinases and serine proteinase inhibitors of seminal plasma and the reproductive tract of turkey (Meleagris gallopavo).
This study examined proteolytic enzymes and serine proteinase inhibitors in turkey seminal plasma with relation to their distribution within the reproductive tract and to yellow semen syndrome (YSS). Proteases of blood plasma, extracts from the reproductive tract, and seminal plasma were analyzed by gelatin zymography. We found a clear regional distribution of proteolytic enzymes in the turkey reproductive tract. Each part was characterized by a unique profile of serine proteolytic enzymes of molecular weights ranging from 29 to 88 kDa. The ductus deferens was found to be a site of very intense proteolytic activity. Two metalloproteases of 58 and 66 kDa were detected in all parts of the reproductive tract and seminal plasma. Using electrophoretic methods for detection of anti-trypsin activity, we found three serine proteinase inhibitors in turkey seminal plasma. Two inhibitors were found in the testis and epididymis and a third in the ductus deferens and seminal plasma. Blood plasma was characterized by the presence of two metalloproteinases and one serine proteinase inhibitor (of low migration rate) that were also detected in the reproductive tract. Amidase and anti-trypsin activities (expressed per gram of protein) differed for yellow and white seminal plasma. We concluded that turkey seminal plasma contains metalloproteases, serine proteinases, and serine proteinase inhibitors. The metalloproteases and one proteinase inhibitor are related to blood proteinases but the other two inhibitors and serine proteinases seem to be unique for the reproductive tract.
Kotłowska
M
M
Department of Semen Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Tuwima 10, Poland.
Kowalski
R
R
Glogowski
J
J
Jankowski
J
J
Ciereszko
A
A
D000818:Animals; D004822:Epididymis / Q000737:chemistry; D018093:Gelatinases / Q000032:analysis*; D008297:Male; D045726:Metalloproteases / Q000032:analysis; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000032:analysis / Q000097:blood; D012098:Reproduction; D012661:Semen / Q000737:chemistry* / Q000201:enzymology; D012697:Serine Endopeptidases / Q000032:analysis; D015842:Serine Proteinase Inhibitors / Q000032:analysis*; D013737:Testis / Q000737:chemistry; D014018:Tissue Distribution; D014361:Trypsin Inhibitors / Q000032:analysis; D014422:Turkeys; D014649:Vas Deferens / Q000737:chemistry
D015842:Serine Proteinase Inhibitors; D014361:Trypsin Inhibitors; D045726:Metalloproteases; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; D018093:Gelatinases