[Raman spectroscopic study on the influence of ultraviolet radiation on calf thymus DNA in aqueous solution].
The Raman spectra of calf thymus DNA in aqueous solution and at 9, 20 and 40 min after ultraviolet radiation were reported. The experimental results proved that though the ultraviolet radiation time was not long, it had a serious influence on the DNA. Only through 9 min of ultraviolet radiation, the peak of 1094 cm(-1) changed into a few weaker peaks, and it was proved that the molecular conformation was changed and the hydrogen bonds were damaged. Some kinds of nucleotides came into being because of the damage to the DNA molecular. Ultraviolet radiation also had influence on adenine, guanine, cytosine and thymine. Among them the purine and pyrimidine were badly damaged. At the same time, our experiment proved that DNA in aqueous solution possesses both A-type structure and B-type structure.
Zhou
Dian-feng
DF
Jiangsu Provincial Key Lab for Optoelectric Technology, Nanjing Normal University, Nanjing 210097, China.
Ke
Wei-zhong
WZ
D000225:Adenine / Q000737:chemistry / Q000528:radiation effects; D000818:Animals; D002417:Cattle; D003596:Cytosine / Q000737:chemistry / Q000528:radiation effects; D004247:DNA / Q000737:chemistry / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D006147:Guanine / Q000737:chemistry / Q000528:radiation effects; D006860:Hydrogen Bonding / Q000528:radiation effects*; D010777:Photochemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013941:Thymine / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays
D006147:Guanine; D003596:Cytosine; D004247:DNA; C427203:calf thymus DNA; D000225:Adenine; D013941:Thymine