[The effects of culture supernatant of bladder tumor cells on formation and function of dendritic cells].
OBJECTIVE
To explore the effects of culture supernatant of bladder tumor cell lines BIU-87 on the development and function of peripheral blood mononuclear cell (PBMC)-derived dendritic cells.


METHODS
The culture supernatant of BIU-87 cells was collected and used as the conditional culture medium for PBMC-derived DCs culture in experiment group. Normal cultured DCs were used as control group. The phenotypes of DCs in experiment group and in control group were analyzed by FACS. The capacity and difference to stimulate allogenetic T cells of PBMC-derived DCs from the two groups were evaluated by MTT colourimetry.


RESULTS
Phenotypic analysis showed that DCs co-cultured with culture supernatant of BIU-87 cells expressed lower levels of CD1a, CD83 and CD86. Moreover, as compared with control group, the maturation of DCs was inhibited, and the capacity to stimulate allgentic T cell proliferation by DCs descended(P<0.05).


CONCLUSIONS
The culture supernatant of BIU-87 cells can inhibit the development and function of DCs. It is a possible reason for the decrease of the number of DCs and the descent of its functional in the patients with bladder tumor.
Sun
Kai
K
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences & Technology, Wuhan 430030, China. kk502716@yahoo.com.cn
Zhou
Si-wei
SW
Cao
Zheng-guo
ZG
Luo
Gang
G
Lu
Xiong-bin
XB
D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000378:metabolism*
D015703:Antigens, CD; D000954:Antigens, Surface; D017077:Culture Media, Conditioned