[The antimicrobial effects of chitosans on oral pathogenic microbes in vitro].
OBJECTIVE
To study the antimicrobial activities of chitosans of different molecular weights and concentrations on oral pathogenic microbes under pH 6.5 in vitro.


METHODS
The inhibition effects of chitosans of different molecular weights and different concentrations (2.0%, 1.5%, 1.0%, 0.5%) on Staphylococcus aureus, Candida albicans, Helicobacter pylori, Streptococcus mutans and Porphyromonas gingivalis in culture were investigated, and the differences of their antimicrobial activities under high temperature and after filtering were compared. One way analysis of variance, randomized block design analysis of variance and t test were used for statistical analysis.


RESULTS
Chitosans of different molecular weights all showed bacteriostatic effects on Staphylococcus aureus, Candida albicans, Helicobacter pylori, Streptococcus mutans and Porphyromonas gingivalis. Low molecular weight chitosans showed the strongest effects, but the high molecular weight ones had strong effects on Streptococcus mutans (P<0.05,0.01). The bacteriostatic effects of chitosans were not affected by high temperatures (P>0.05).


CONCLUSIONS
Chitosans have inhibitory effects on oral pathogenic microbes under pH 6.5.
Deng
Jing
J
Department of Stomotology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China. dengjing6666@yahoo.com.cn
Fu
Da-Yong
DY
Song
Wen-Bin
WB
Yuan
Chang-Qing
CQ
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects; D008970:Molecular Weight; D016966:Porphyromonas gingivalis / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013295:Streptococcus mutans / Q000187:drug effects
D000890:Anti-Infective Agents; D048271:Chitosan