Dendrimer modified magnetite nanoparticles for protein immobilization.
A cascading polyamidoamine (PAMAM) dendrimer was synthesized on the surface of magnetite nanoparticles to allow enhanced immobilization of bovine serum albumin (BSA). Characterization of the synthesis revealed exponential doubling of the surface amine from generations one through four starting with an amino silane initiator. Furthermore, transmission electron microscopy (TEM) revealed clear dispersion of the dendrimer-modified magnetite nanoparticles in methanol solution. The dendrimer-modified magnetite nanoparticles were used to carry out magnetic immobilization of BSA. BSA immobilizing efficiency increased with increasing generation from one to five and BSA binding amount of magnetite nanoparticles modified with G5 dendrimer was 7.7 times as much as that of magnetite nanoparticles modified with only aminosilane. There are two major factors that improve the BSA binding capacity of dendrimer-modified magnetite nanoparticles: one is that the increased surface amine can be conjugated to BSA by a chemical bond through glutaraldehyde; the other is that the available area has increased due to the repulsion of surface positive charge.
Pan
Bi-Feng
BF
Engineering Research Center for Nano Science and Technology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, People's Republic of China.
Gao
Feng
F
Gu
Hong-Chen
HC
D050091:Dendrimers; D005290:Ferric Compounds / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000138:chemical synthesis / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry*; D011437:Propylamines / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
D050091:Dendrimers; D005290:Ferric Compounds; C104700:PAMAM Starburst; D011073:Polyamines; D011437:Propylamines; D011506:Proteins; D012821:Silanes; C088294:3-aminopropyltrimethoxysilane; C000499:ferric oxide; D012710:Serum Albumin, Bovine; D052203:Ferrosoferric Oxide