JNK signaling pathway required for wound healing in regenerating Drosophila wing imaginal discs.
We have examined wound healing during regeneration of Drosophila wing imaginal discs fragments by confocal microscopy and assessed the role of components of the JNK pathway in this process. After cutting, columnar and peripodial epithelia cells at the wound edge start to close the wound through formation and contraction of an actin cable. This is followed by a zipping process through filopodial protrusions from both epithelia knitting the wound edges from proximal to distal areas of the disc. Activation of the JNK pathway is involved in such process. puckered (puc) expression is induced in several rows of cells at the edge of the wound, whereas absence of JNK pathway activity brought about by hemipterous, basket, and Dfos mutants impair wound healing. These defects are accompanied by lowered or loss of expression of puc. In support of a role of puc in wound healing, hep mutant phenotypes are rescued by reducing puc function, whereas overexpression of puc inhibits wound healing. Altogether, these results demonstrate a role for the JNK pathway in imaginal disc wound healing, similar to that reported for other healing processes such as embryonic dorsal closure, thoracic closure, and adult epithelial wound healing in Drosophila. Differences with such processes are also highlighted.
Bosch
Manel
M
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain.
Serras
Florenci
F
Martín-Blanco
Enrique
E
Baguñà
Jaume
J
D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000502:physiology; D004628:Embryonic Structures / Q000473:pathology* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*; D014921:Wings, Animal / Q000473:pathology / Q000502:physiology
D029721:Drosophila Proteins; C435749:kay protein, Drosophila; D048031:JNK Mitogen-Activated Protein Kinases; C117694:Hep protein, Drosophila; D020929:Mitogen-Activated Protein Kinase Kinases; C110854:puc protein, Drosophila; D010749:Phosphoprotein Phosphatases