Localization of poly-L-lysine-photosensitizer conjugate  in nucleus.
The cellular uptake and photocytotoxity of poly-l-lysine (pL)-chlorin e6 (Ce6) conjugate were investigated. The cellularuptake of pL-Ce6 conjugate for HeLa cells was much higher than that of Ce6, and pL-Ce6 conjugate had high binding affinityfor HeLa cells. pL-Ce6 conjugate was accumulated in the nucleus of HeLa cells, and the effective photocytotoxity wasobserved by the irradiation.
Ogura
Shun-ichiro
S
Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Yazaki
Keiko
K
Yamaguchi
Kazuki
K
Kamachi
Toshiaki
T
Okura
Ichiro
I
D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002735:Chlorophyllides; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D017319:Photosensitizing Agents / Q000032:analysis / Q000378:metabolism*; D011107:Polylysine / Q000032:analysis / Q000378:metabolism*; D011166:Porphyrins / Q000032:analysis / Q000378:metabolism
D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; D011107:Polylysine; C062985:phytochlorin