Structural and genetic characterization of the Shigella boydii type 10 and type 6 O antigens.
Comparison of the O antigens of Shigella boydii types 10 and 6 by chemical analysis and nuclear magnetic resonance spectroscopy showed that their structures are similar, with the only difference being the presence or absence of d-ribofuranose, which is the immunodominant sugar in S. boydii type 10. In S. boydii type 6, a residue previously reported as alpha-d-GlcpA, was shown to be beta-d-GlcpA as in S. boydii type 10. S. boydii types 10 and 6 are reported not to cross-react serologically, and the role of d-ribofuranose in the specificity of S. boydii was confirmed by making a mutant of type 10 that lacked d-ribofuranose. However, S. boydii type 11, which has a d-ribofuranose but with different linkage does show cross-reaction with type 10. The O-antigen gene loci of S. boydii types 10 and 6 were shown to be virtually identical except that orf8 (wbaM), which was confirmed as the ribofuranosyltransferase gene, is interrupted by IS629 in type 6. Therefore, it is proposed that the O-antigen gene cluster of S. boydii type 6 was derived from type 10 by an IS element insertion.
Senchenkova
Sof'ya N
SN
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Feng
Lu
L
Yang
Jinghua
J
Shashkov
Alexander S
AS
Cheng
Jiansong
J
Liu
Dan
D
Knirel
Yuriy A
YA
Reeves
Peter R
PR
Jin
Qi
Q
Ye
Qiang
Q
Wang
Lei
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry / Q000235:genetics*; D012761:Shigella boydii / Q000235:genetics* / Q000276:immunology
D019081:O Antigens