title: Fibroblast growth factor receptor-1 expression is required for hematopoietic but not endothelial cell development.
abstract: OBJECTIVE
  The purpose of this study was to clarify the role of fibroblast growth factors (FGFs) and FGF receptors (FGFRs) in hematopoietic/endothelial development.
  
  
  RESULTS
  Using several different FGFR-1-specific antibodies and FGFR-1 promoter-driven LacZ activity, we show that FGFR-1 is expressed and active as a tyrosine kinase in a subpopulation of endothelial cells (approximately 20% of the endothelial pool) during development in embryoid bodies. In agreement, in stem cell-derived teratomas, expression of FGFR-1 was detected in some but not all vessels. The FGFR-1 expressing endothelial cells were mitogenically active in the absence and presence of vascular endothelial growth factor (VEGF). Expression of FGFR-1 in endothelial cell precursors was not required for vascular development, and vascularization was enhanced in FGFR-1-deficient embryoid bodies compared with wild-type stem cells. In contrast, hematopoietic development was severely disturbed, with reduced expression of markers for primitive and definitive hematopoiesis.
  
  
  CONCLUSIONS
  Our data show that FGFR-1 is expressed in early hematopoietic/endothelial precursor cells, as well as in a subpool of endothelial cells in tumor vessels, and that it is critical for hematopoietic but not for vascular development.
authors.affiliation: Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
authors.forename: Peetra Ulrica
authors.initials: PU
authors.lastname: Magnusson
authors.forename: Roberto
authors.initials: R
authors.lastname: Ronca
authors.forename: Patrizia
authors.initials: P
authors.lastname: Dell'Era
authors.forename: Pia
authors.initials: P
authors.lastname: Carlstedt
authors.forename: Lars
authors.initials: L
authors.lastname: Jakobsson
authors.forename: Juha
authors.initials: J
authors.lastname: Partanen
authors.forename: Anna
authors.initials: A
authors.lastname: Dimberg
authors.forename: Lena
authors.initials: L
authors.lastname: Claesson-Welsh
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000196:embryology / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007763:Lac Operon; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000502:physiology; D011401:Promoter Regions, Genetic; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics* / Q000378:metabolism; D013724:Teratoma / Q000098:blood supply
chemical_list: D051496:Receptor, Fibroblast Growth Factor, Type 1