title: Cooperation of metal-ion fixation and target-site activation for efficient site-selective RNA scission.
abstract: Iminodiacetate-DNA conjugates and acridine-DNA conjugates were synthesized and combined for site-selective RNA hydrolysis by Lu(III). When these conjugates form a ternary complex with complementary RNA, the Lu(III)-iminodiacetate complex is placed near the target phosphodiester linkage of RNA which is in front of the acridine and is activated by noncovalent interactions. The site-selective hydrolysis by these combinations is several times as fast as that achieved by combining unmodified DNA (without iminodiacetate) and the acridine-DNA conjugate.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo, 153-8904, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Kuzuya
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.forename: Takuro
authors.initials: T
authors.lastname: Sasayama
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D007477:Ions / Q000737:chemistry; D008187:Lutetium / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D007477:Ions; D008187:Lutetium; D012313:RNA