Central vagal stimulation activates enteric cholinergic neurons in the stomach and VIP neurons in the duodenum in conscious rats.
The influence of central vagal stimulation induced by 2h cold exposure or intracisternal injection of thyrotropin-releasing hormone (TRH) analog, RX-77368, on gastro-duodenal enteric cholinergic neuronal activity was assessed in conscious rats with Fos and peripheral choline acetyltransferase (pChAT) immunoreactivity (IR). pChAT-IR was detected in 68%, 70% and 73% of corpus, antrum and duodenum submucosal neurons, respectively, and in 65% of gastric and 46% of duodenal myenteric neurons. Cold and RX-77368 induced Fos-IR in over 90% of gastric submucosal and myenteric neurons, while in duodenum only 25-27% of submucosal and 50-51% myenteric duodenal neurons were Fos positive. In the stomach, cold induced Fos-IR in 93% of submucosal and 97% of myenteric pChAT-IR neurons, while in the duodenum only 7% submucosal and 5% myenteric pChAT-IR neurons were Fos positive. In the duodenum, cold induced Fos in 91% of submucosal and 99% of myenteric VIP-IR neurons. RX-77368 induces similar percentages of Fos/pChAT-IR and Fos/VIP-IR neurons. These results indicate that increased central vagal outflow activates cholinergic neurons in the stomach while in the duodenum, VIP neurons are preferentially stimulated.
Yuan
Pu-Qing
PQ
CURE: Digestive Diseases Research Center, VA Greater Los Angeles Healthcare System, Digestive Diseases Division, Department of Medicine and Brain Research Institute, University of California Los Angeles, Los Angeles, CA 90073, USA. pqyuan@ucla.edu
Kimura
Hiroshi
H
Million
Mulugeta
M
Bellier
Jean-Pierre
JP
Wang
Lixin
L
Ohning
Gordon V
GV
Taché
Yvette
Y
D000818:Animals; D003243:Consciousness; D004386:Duodenum / Q000294:innervation*; D005753:Gastric Mucosa / Q000187:drug effects / Q000294:innervation*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000294:innervation*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000294:innervation*; D013972:Thyrotropin / Q000494:pharmacology; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology*
D013972:Thyrotropin