The usefulness of calyculin a for cytogenetic prenatal diagnosis.
An increased number of chromosome plates can be obtained by use of calyculin A (CLA). CLA is an inhibitor of protein phosphatases (type 1 and type 2A serine/threonine). Inactivation of these phosphatases leads to premature chromosome condensation (PCC) in all phases of the cell cycle; thus, it is possible to investigate both metaphase and G(2)-PCC chromosomes. Amniotic fluid (AF) cultures were treated with calyculin A (CLA). GTG banding was obtained. Using this method it is possible to investigate all cell cycle phases, GTG banding, chromosomal breaks, and rates of PCD on the same preparation. Analyses of AF cultures treated with CLA allow complex studies on fetal genetic material. This work presents potential usefulness of CLA for cytogenetic prenatal diagnosis.
Srebniak
Malgorzata I
MI
Silesian Medical Academy, Department of General Medical Biology, ul. Jordana 19, 41-808 Zabrze, Poland. srebna@poczta.onet.pl
Trapp
Gizela G
GG
Wawrzkiewicz
Angelika K
AK
Kaźmierczak
Wojciech
W
Wiczkowski
Andrzej K
AK
D000653:Amniotic Fluid / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D002503:Centromere / Q000648:ultrastructure; D002871:Chromosome Banding; D019457:Chromosome Breakage; D002877:Chromosomes, Human / Q000648:ultrastructure*; D020732:Cytogenetic Analysis; D016195:G2 Phase; D006801:Humans; D007621:Karyotyping; D008387:Marine Toxins; D010080:Oxazoles; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D011296:Prenatal Diagnosis / Q000379:methods*
D008387:Marine Toxins; D010080:Oxazoles; C059041:calyculin A; D010749:Phosphoprotein Phosphatases