DNA polymerase lambda mediates a back-up base excision repair activity in extracts of mouse embryonic fibroblasts.
Mammalian DNA polymerase (pol) lambda is a member of the X-family of DNA polymerases and has striking enzymatic and structural similarities to mammalian DNA pol beta. Because pol beta provides two important enzymatic activities for base excision repair (BER), we examined whether pol lambda might also contribute to BER. We used extracts from mouse embryonic fibroblasts representing wild-type and null genotypes for pol beta and pol lambda. In combination with neutralizing antibodies against pol beta and pol lambda, our results show a BER deficiency in the pol lambda -/- cell extract compared with extract from isogenic wild-type cells. In addition, the pol lambda antibody strongly reduced in vitro BER in the pol beta -/- cell extract. These data indicate that pol lambda is able to contribute to BER in mouse fibroblast cell extract.
Braithwaite
Elena K
EK
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
Prasad
Rajendra
R
Shock
David D
DD
Hou
Esther W
EW
Beard
William A
WA
Wilson
Samuel H
SH
D000818:Animals; D002460:Cell Line; D019951:DNA Polymerase beta / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D004260:DNA Repair / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology*; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout
C415367:DNA polymerase beta2; D019951:DNA Polymerase beta