title: Characterization of ionic currents in human mesenchymal stem cells from bone marrow.
abstract: This study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca(2+)-activated K(+) channel (I(KCa)) blocker iberiotoxin, a transient outward K(+) current (I(to)) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K(+) current (IK(DR))-like ether-à-go-go (eag) K(+) channel. In addition, tetrodotoxin-sensitive sodium current (I(Na.TTX)) and nifedipine-sensitive L-type Ca(2+) current (I(Ca.L)) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for I(KCa), Kv4.2 and Kv1.4 for I(to), heag1 for IK(DR), hNE-Na for I(Na.TTX), and CACNAIC for I(Ca.L). These results demonstrate that multiple functional ion channel currents--that is, I(KCa), I(to), heag1, I(Na.TTX), and I(Ca.L)--are expressed in hMSCs from bone marrow.
authors.affiliation: Department of Medicine, Research Centre on Heart, Brain, Hormones and Healthy Aging, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. grli@hkucc.hku.hk
authors.forename: Gui-Rong
authors.initials: GR
authors.lastname: Li
authors.forename: Haiying
authors.initials: H
authors.lastname: Sun
authors.forename: Xiuling
authors.initials: X
authors.lastname: Deng
authors.forename: Chu-Pak
authors.initials: CP
authors.lastname: Lau
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D051638:Ether-A-Go-Go Potassium Channels; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000502:physiology*; D051665:Kv1.4 Potassium Channel; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D051036:Large-Conductance Calcium-Activated Potassium Channels; D008564:Membrane Potentials / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D062556:NAV1.7 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins / Q000235:genetics; D009543:Nifedipine / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000494:pharmacology; D015221:Potassium Channels / Q000235:genetics; D024681:Potassium Channels, Calcium-Activated / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051672:Shal Potassium Channels; D015222:Sodium Channels / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: C079146:CACNA1C protein, human; D020746:Calcium Channels, L-Type; D051638:Ether-A-Go-Go Potassium Channels; D007473:Ion Channels; C496977:KCNA4 protein, human; C497018:KCND2 protein, human; C494841:KCNMA1 protein, human; D051665:Kv1.4 Potassium Channel; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D051036:Large-Conductance Calcium-Activated Potassium Channels; D062556:NAV1.7 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; D010455:Peptides; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; C486866:SCN9A protein, human; D051672:Shal Potassium Channels; D015222:Sodium Channels; D013779:Tetrodotoxin; C064719:iberiotoxin; D015761:4-Aminopyridine; D009543:Nifedipine