title: Extended-spectrum beta-lactamase-producing Shiga toxin gene (Stx1)-positive Escherichia coli O26:H11: a new concern.
abstract: Escherichia coli strain TUM2139 was isolated from a stool sample from a 9-year-old girl on 16 June 2004. This strain was categorized as Shiga toxin-producing Escherichia coli (STEC) because the Shiga-like toxin gene stx(1) was detected by immunochromatography and PCR assay. The strain was highly resistant to cefotaxime (256 microg/ml) and was also resistant to cefepime, cefpodoxime, ceftriaxone, and aztreonam. In the presence of 4 microg of clavulanic acid per ml, the MIC of cefotaxime decreased to < or =0.12 microg/ml, indicating that this strain was an extended-spectrum beta-lactamase (ESBL) producer. Cefotaxime resistance was transferred to E. coli C600 by conjugation at a frequency of 3.0 x 10(-6). A PCR assay was performed with primer sets specific for TEM-type and SHV-type ESBLs and for the CTX-M-2 (Toho-1), CTX-M-3, and CTX-M-9 groups of ESBLs. A specific signal was observed with the primer set specific for the CTX-M-9 group of beta-lactamases. This beta-lactamase was confirmed to be the ESBL CTX-M-18 by DNA sequencing. This is the first report of an ESBL-producing STEC isolate.
authors.affiliation: Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 1438540 Tokyo, Japan. yoishii@med.toho-u.ac.jp
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ishii
authors.forename: Soichiro
authors.initials: S
authors.lastname: Kimura
authors.forename: Jimena
authors.initials: J
authors.lastname: Alba
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Shiroto
authors.forename: Masanobu
authors.initials: M
authors.lastname: Otsuka
authors.forename: Naotaka
authors.initials: N
authors.lastname: Hashizume
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Tamura
authors.forename: Keizo
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D002648:Child; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D022621:Shiga Toxin / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D022621:Shiga Toxin; D001618:beta-Lactamases