Conservation of the deadenylase activity of proteins of the Caf1 family in human.
The yeast Pop2 protein, belonging to the eukaryotic Caf1 family, is required for mRNA deadenylation in vivo. It also catalyzes poly(A) degradation in vitro, even though this property has been questioned. Caf1 proteins are related to RNase D, a feature supported by the recently published structure of Pop2. Yeast Pop2 contains, however, a divergent active site while its human homologs harbor consensus catalytic residues. Given these differences, we tested whether its deadenylase activity is conserved in the human homologs Caf1 and Pop2. Our data demonstrate that both human factors degrade poly(A) tails indicating their involvement in mRNA metabolism.
Bianchin
Claire
C
Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, CNRS UPR2167 Associée à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, France.
Mauxion
Fabienne
F
Sentis
Stéphanie
S
Séraphin
Bertrand
B
Corbo
Laura
L
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D016415:Sequence Alignment; D014157:Transcription Factors
C489780:CNOT8 protein, human; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D011061:Poly A; D012260:Ribonucleases; C079009:POP2 protein, S cerevisiae