Transient expression of MIDC-8 in the normal mouse brain.
In this study, we have immunohistochemically characterized the expression of mononuclear phagocyte markers CD14, CD36, CD68, CD204 and MARCO by parenchymal microglia in the developing and adult mouse brain. We further investigated whether these cells express two well-characterized phenotypic markers of dendritic cells: CD205 (DEC-205/NLDC-145) and MIDC-8 antigen. Our results confirm the lack of expression of dendritic cell markers by microglia. We noted that these cells do not appear to express markers associated with monocytes and macrophages during the course of development, but do express CD68 and CD204 antigens in the adult. Unexpectedly, we also noted the transient expression of MIDC-8 antigen on cells within the medial ganglionic eminence and by neuroepithelial cells lining the lateral ventricles and in the medial lemniscus between E15 and E19. We discuss this finding in the context of neural and haematopoietic differentiation.
Rezaie
Payam
P
Department of Biological Sciences, Faculty of Science, The Open University, Walton Hall, Milton Keynes MK7 6AA, UK. p.rezaie@open.ac.uk
Corbisiero
Vanessa
V
Male
David
D
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D018955:CD36 Antigens / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D037181:Lectins, C-Type / Q000096:biosynthesis; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000096:biosynthesis
D015703:Antigens, CD; D018955:CD36 Antigens; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface