Electrical inhibition of lens epithelial cell proliferation: an additional factor in secondary cataract?
Cataract is the most common cause of blindness but is at least curable by surgery. Unfortunately, many patients gradually develop the complication of posterior capsule opacification (PCO) or secondary cataract. This arises from stimulated cell growth within the lens capsule and can greatly impair vision. It is not fully understood why residual lens epithelial cell growth occurs after surgery. We propose and show that cataract surgery might remove an important inhibitory factor for lens cell growth, namely electric fields. The lens generates a unique pattern of electric currents constantly flowing out from the equator and entering the anterior and posterior poles. We show here that cutting and removing part of the anterior capsule as in cataract surgery significantly decreases the equatorial outward electric currents. Application of electric fields in culture inhibits proliferation of human lens epithelial cells. This inhibitory effect is likely to be mediated through a cell cycle control mechanism that decreases entry of cells into S phase from G1 phase by decreasing the G1-specific cell cycle protein cyclin E and increasing the cyclin-Cdk complex inhibitor p27kip1. Capsulorrhexis in vivo, which reduced endogenous lens electric fields, significantly increased LEC growth. This, together with our previous findings that electric fields have significant effects on the direction of lens cell migration, points to a controlling mechanism for the aberrant cell growth in posterior capsule opacification. A novel approach to control growth of lens epithelial cells using electric fields combined with other controlling mechanisms may be more effective in the prevention and treatment of this common complication of cataract surgery.
Wang
Entong
E
Department of Biomedical Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
Reid
Brian
B
Lois
Noemi
N
Forrester
John V
JV
McCaig
Colin D
CD
Zhao
Min
M
D000818:Animals; D002386:Cataract / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D002387:Cataract Extraction / Q000009:adverse effects*; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D004553:Electric Conductivity; D004560:Electricity; D004847:Epithelial Cells / Q000473:pathology* / Q000502:physiology; D007903:Lens Capsule, Crystalline / Q000473:pathology* / Q000503:physiopathology; D008839:Microelectrodes; D011183:Postoperative Complications / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley