[Effect of photodynamic therapy with metalloporphyrin compound on human prostate cancer PC-3 cells in vitro].
OBJECTIVE
To investigate the effect of the photodynamic therapy (PDT) with the new water-soluble metalloporphyrin compound on human prostate cancer PC-3 cells in vitro and the anticancer mechanism of PDT.


METHODS
The new water-soluble manganese, 5,10,15, 20-tetra (N-methyl4-pyridyl) porphinato (2-) tetraiodide salt, was synthesized. The PC-3 cells were treated with the compound of serial concentrations(0, 0.1, 1, 1.0 micromol/L) followed by irradiation of different dosages of visible light. The techniques of MTT and Annexin-V/propidium iodide double-labeled flow cytometry (FCM) were applied to measuring the inhibitory effect of the compound on the growth activity and apoptosis of the cells.


RESULTS
When the metalloporphyrin compound concentration was within 10 micromol/L and the irradiation time was within 30 min, the water-soluble metalloporphyrin compound had a significant inhibitory effect on the proliferation of PC-3 cells and induced PC-3 cell apoptosis, and the effects depended greatly on metalloporphyrin concentration and illumination dosages. Higher concentrations and dosages induced the death of the majority of PC-3 cells.


CONCLUSIONS
The PDT of the water-soluble metalloporphyrin compound followed by light irradiation has a distinctive killing effect on PC-3 cells in vitro, and the rates of proliferation inhibition and cell apoptosis are correlated with metalloporphyrin concentration and the dosages of light irradiation. The results suggest that the mechanism of metalloporphyrin PDT may be involved with the induction of apoptosis in human prostate cancer cells.
Mi
Qi-Wu
QW
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Cao
Zheng-Guo
ZG
Jia
Tao
T
Zhou
Si-Wei
SW
Liu
Ji-Hong
JH
Song
Xiao-Dong
XD
Li
Zao-Ying
ZY
Ye
Zhang-Qun
ZQ
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008297:Male; D008665:Metalloporphyrins / Q000494:pharmacology*; D010778:Photochemotherapy; D011471:Prostatic Neoplasms / Q000473:pathology*
D008665:Metalloporphyrins; C519330:manganese 5,10,15,20-tetra (N-methyl4-pyridyl) porphinato (2-) tetraiodide salt