title: Video microscopy as an alternative method for somatic cell count in milk.
abstract: This paper presents an alternative method to estimate somatic cell count (SCC) in cows' milk. SCC is an important indicator in the detection of inflammatory reactions within the udder in cows and Direct Optical Microscopy (DOM) is the present reference method for SCC but, owing to its dependence on human operators, it is extremely costly, time-consuming and potentially subjective. The industrial method of choice is Epifluorescence (EF), which has the potential for impressive throughput and acceptable precision, but requires huge inversions and handling of highly toxic reactives and waste. In this paper, an advantageous method that involves application of a low-cost Video Microscopy (VM) system is analysed and discussed, including a comparison between DOM and VM, and an example of application of both methods to evaluate EF counts. We conclude that VM is sufficiently precise and very cheap to implement and operate.
authors.affiliation: Dept CC Agroforestales, Universidad de Valladolid, 34004 Palencia, Spain. baro@agro.uva.es
authors.forename: Jesús A
authors.initials: JA
authors.lastname: Baro
authors.forename: Patricia
authors.initials: P
authors.lastname: Roldán
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Carleos
authors.forename: Gustavo J
authors.initials: GJ
authors.lastname: Grillo
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Pérez
mesh_terms: D000818:Animals; D002417:Cattle; D003612:Dairying / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D018715:Microscopy, Video / Q000379:methods; D008892:Milk / Q000166:cytology*; D008991:Monitoring, Physiologic / Q000379:methods; D015203:Reproducibility of Results