title: WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor.
abstract: Internalisation of the pathogenic bacterium Listeria monocytogenes involves interactions between the invasion protein InlB and the hepatocyte growth factor receptor, Met. Using colocalisation studies, dominant-negative constructs and small interfering RNA (siRNA), we demonstrate a cell-type-dependent requirement for various WASP-related proteins in Listeria entry and InlB-induced membrane ruffling. The WAVE2 isoform is essential for InlB-induced cytoskeletal rearrangements in Vero cells. In HeLa cells, WAVE1, WAVE2 and N-WASP cooperate to promote these processes. Abi1, a key component of WAVE complexes, is recruited at the entry site in both cell types and its inactivation by RNA interference impairs InlB-mediated processes. Ena/VASP proteins also play a role in Listeria internalization, and their deregulation by sequestration or overexpression, modifies actin cups beneath entering particles. Taken together, these results identify the WAVE complex, N-WASP and Ena/VASP as key effectors of the Met signalling pathway and of Listeria entry and highlight the existence of redundant and/or cooperative functions among WASP-family members.
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, INSERM U604, INRA USC2020, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Bierne
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Miki
authors.forename: Metello
authors.initials: M
authors.lastname: Innocenti
authors.forename: Giorgio
authors.initials: G
authors.lastname: Scita
authors.forename: Frank B
authors.initials: FB
authors.lastname: Gertler
authors.forename: Tadaomi
authors.initials: T
authors.lastname: Takenawa
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D014709:Vero Cells; D051300:Wiskott-Aldrich Syndrome Protein Family; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal
chemical_list: C513733:ABI1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; C092744:ENA-VASP proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C495771:WASL protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal; C095768:inlB protein, Listeria monocytogenes; D019859:Proto-Oncogene Proteins c-met