Sequential affinity chromatography miniaturized within a "lab-on-valve" system.
An automatically renewable microcolumn, subjected to operation by programmable flow, is presented and for the first time used for separation and quantification of biomolecules on Sepharose Protein A beads, with absorbance measurement at 280 nm and a detection limit of 6 ng mouse IgG microL(-1).
Erxleben
Holger
H
Innovatis AG, Im Technologiezentrum, Meisenstr 96, 33607 Bielefeld, Germany. herxleben@hotmail.com
Ruzicka
Jaromir
J
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D017022:Flow Injection Analysis; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008832:Microchemistry / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000032:analysis
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine