Use of microsatellite markers and gene dosage to quantify gene copy numbers in Candida albicans.
With microsatellite marker typing, the number of alleles must be known for calculation of allelic frequencies in the diploid Candida albicans for a given locus. We describe a gene dosage with a double real-time PCR. Such a dosage should also be useful in exploring the loss of heterozygosity in C. albicans.
Costa
J-M
JM
Laboratoire de Parasitologie-Mycologie, Hôpital H. Mondor, 51 avenue du Maréchal DeLattre de Tassigny, 94010 Créteil, France.
Eloy
O
O
Botterel
F
F
Janbon
G
G
Bretagne
S
S
D002176:Candida albicans / Q000235:genetics*; D018628:Gene Dosage; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction