[Temperature dependence of collagen by Raman spectra].
Raman spectra of collagen I at different temperature were obtained. There was no change at 1003 cm(-1) line, 1302 cm(-1) line moved to a higher wave number, but other lines moved to lower wave number when temperature increased. In addition, the authors observed the temperature dependence of Raman intensity and four denaturation points at 0, 40, 68 and 90 degrees C respectively. The points at 40 and 68 degrees C are in agreement with the experimental data by DSC and SHG. The point at 0 degrees C might be frozen transition; the point at 90 degrees C might be related to the damage of secondary structure. When heated to 150 degrees C, the Raman intensity of all bands decreased rapidly and many lines disappeared.
Dong
Rui-xin
RX
Physics Department of Liaocheng University, Liaocheng 252059, China.
Yan
Xun-ling
XL
Jiang
Shan
S
Liu
Mei-jie
MJ
Liu
Sheng-gang
SG
D000818:Animals; D024042:Collagen Type I / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013696:Temperature
D024042:Collagen Type I