title: Sequential affinity chromatography miniaturized within a "lab-on-valve" system.
abstract: An automatically renewable microcolumn, subjected to operation by programmable flow, is presented and for the first time used for separation and quantification of biomolecules on Sepharose Protein A beads, with absorbance measurement at 280 nm and a detection limit of 6 ng mouse IgG microL(-1).
authors.affiliation: Innovatis AG, Im Technologiezentrum, Meisenstr 96, 33607 Bielefeld, Germany. herxleben@hotmail.com
authors.forename: Holger
authors.initials: H
authors.lastname: Erxleben
authors.forename: Jaromir
authors.initials: J
authors.lastname: Ruzicka
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D017022:Flow Injection Analysis; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008832:Microchemistry / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine