Inhibitory activity of a ceramide library on interleukin-4 production from activated T cells.
Allergic diseases are hypersensitivity disorders associated with the production of specific immunoglobulin E (IgE) to environmental allergens. Interleukin (IL)-4, produced primarily by CD4(+) T cells, is an important stimulus for the switch of the antibody isotype to IgE in both mice and humans. In this study we investigated the inhibitory activity of IL-4 production in activated T cells by screening ceramide derivatives prepared by solid phase combinatorial chemistry. Many ceramide derivatives significantly inhibited IL-4 production in T cells. In particular, ceramide derivatives with a lauroyl group showed strong inhibitory activities on IL-4 production in both phorbol 12-myristate 13-acetate (PMA)-activated EL4 T cells and antigen-primed cells, suggesting that they can be used as compounds for the development of anti-allergic agents.
Park
Jin
J
Department of Pharmacy, College of Pharmacy, Chonnam National University, Gwangju 500-757, Republic of Korea.
Li
Qian
Q
Chang
Young-Tae
YT
Kim
Tae Sung
TS
D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002518:Ceramides / Q000737:chemistry / Q000494:pharmacology*; D020650:Combinatorial Chemistry Techniques; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D015847:Interleukin-4 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D018926:Anti-Allergic Agents; D002518:Ceramides; D012333:RNA, Messenger; D015847:Interleukin-4; C016658:phorbolol myristate acetate; D013755:Tetradecanoylphorbol Acetate