[In vitro effect on the differentiation and maturation of monocyte-derived dendritic cells by mannan-binding lectin].
OBJECTIVE
To explore the effect of mannan-binding lectin (MBL) on the differentiation and maturation of human peripheral blood monocyte-derived dendritic cells (MoDCs).


METHODS
After MoDCs was stimulated with human natural MBL, MoDC's morphology was observed under inverted microscope and the expressions of CD1a, CD83, CD40, CD80, CD86 and HLA-DR on MoDCs were analyzed by FACS. The ability of MoDCs to stimulate the proliferation of allogenic T cells was detected by (3)H-TdR incorporation. The ability of MoDCs to up-take antigens was evaluated by zymosan granule phagocytosis test. The levels of IL-12 and TNF-alpha in the culture supernatant of MoDCs were determined by ELISA.


RESULTS
The expressions of CD1a, CD83, CD40, CD80, CD86 and HLA-DR on the MoDCs were up-regulated by MBL. The ability of MoDCs to up-take zymosan granules decreased and the proliferation of native T cells induced by MoDCs was enhanced. MBL stimulated the production of IL-12 by MoDCs, but had no such effect on TNF-alpha secretion.


CONCLUSIONS
MBL can induce differentiation and maturation of DCs in vitro, suggesting that MBL possibly participate in the adaptive immune response through modulation of functions of DCs.
Chen
Yue
Y
Department of Immunology, Southern Medical University, Guangzhou 510515, China. chenyue@fimmu.com
Chen
Zheng-liang
ZL
Zuo
Da-ming
DM
Qiu
Yi-gang
YG
Zhang
Li-yun
LY
D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D037601:Mannose-Binding Lectin / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D015054:Zymosan / Q000378:metabolism
D037601:Mannose-Binding Lectin; D015054:Zymosan