title: Reproductive effects in German cockroaches by ecdysteroid agonist RH-0345, juvenile hormone analogue methoprene and carbamate benfuracarb.
abstract: Blatta germanica is the more prevalent cockroach species in Algeria. In the present study, we tested the effect on reproduction in B. germanica of two insect growth regulators, RH-0345, a benzoylhydrazine analogue that mimics the action of 20-hydroxyecdysone, and methoprene, one of the most commercially important juvenile hormone analogues, and a novel carbamate insecticide, benfuracarb. The compounds were applied topically (10 and 20 microg/insect for RH-0345, and 1 and 10 microg/insect for methoprene) or orally administrated (at 2% for benfuracarb) on newly emerged females and evaluated on reproductive events during the adult life (2, 4 and 6 days). Treatment with RH-0345 and benfuracarb reduced significantly the number of oocytes, the size and the volume of the basal oocyte during the experimental period. Methoprene distorted the ovarian development since it caused a significant reduction in the number of oocytes at 2, 4 and 6 days for the two tested doses, and an increase in oocyte size at 2, 4 and 6 days with 1 microg and a decrease with 10 microg. In a second series of experiments, the effects of these compounds were assayed on the ovarian proteins. Data from biochemical analysis revealed that RH-0345 and benfuracarb reduced the ovarian amounts of proteins, while treatment with methoprene increased it during the sexual maturation.
authors.affiliation: Laboratoire de Biologie Animale Appliquée, Universite d'Annaba, 23000 Annaba, Algérie.
authors.forename: A
authors.initials: A
authors.lastname: Maiza
authors.forename: S
authors.initials: S
authors.lastname: Kilani
authors.forename: J P
authors.initials: JP
authors.lastname: Farine
authors.forename: G
authors.initials: G
authors.lastname: Smagghe
authors.forename: N
authors.initials: N
authors.lastname: Aribi
authors.forename: N
authors.initials: N
authors.lastname: Soltani
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001565:Benzoates / Q000633:toxicity*; D001572:Benzofurans / Q000633:toxicity*; D020048:Blattellidae / Q000187:drug effects / Q000254:growth & development*; D005260:Female; D006834:Hydrazines / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007605:Juvenile Hormones; D008726:Methoprene / Q000633:toxicity*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects; D010053:Ovary / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000633:toxicity*
chemical_list: D001565:Benzoates; D001572:Benzofurans; D006834:Hydrazines; D007306:Insecticides; D007605:Juvenile Hormones; D015091:beta-Alanine; C096128:benfuracarb; D008726:Methoprene; C437546:N-4-chlorobenzoyl-N'-benzoyl-N'-tert-butylhydrazine