Effect of desmopressin (DDAVP) on platelet membrane glycoprotein expression in patients with von Willebrand's disease.
When patients with von Willebrand's disease were given a single injection of desmopressin (0.4 microg/kg body weight), there was a considerable increase in platelet reactivity (from 0.95 +/- 0.19 to 1.44 +/- 0.42; p = 0.0033). On flow cytometry, increased glycoprotein Ib/IX expression in the platelets was found after the desmopressin injection; when phycoerythrin-marked anti-CD62 antibodies were used, the mean fluorescence rose from 428.9 +/- 56.6 to 440.7 +/- 51.4 (p = 0.0056), and from 425.9 +/- 55.0 to 437.4 +/- 53.9 (p = 0.0018) when phycoerythrin-marked anti-thrombospondin antibodies were used. Apart from the rise in the von Willebrand factor, this could explain the increased platelet reactivity. However, the surface expression of CD62, CD63 and thrombospondin on platelets did not change following the desmopressin injection.
Gordz
S
S
Department of Clinical Haemostaseology and Transfusion Medicine, University of the Saarland, Hamburg, Germany.
Mrowietz
C
C
Pindur
G
G
Park
J W
JW
Jung
F
F
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D005260:Female; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis*; D014842:von Willebrand Diseases / Q000097:blood
D015703:Antigens, CD; D006490:Hemostatics; D010980:Platelet Membrane Glycoproteins; D003894:Deamino Arginine Vasopressin