Changes of calcitonin gene-related peptide in primary sensory neurons and their central branch after nerve root compression of the dog.
OBJECTIVE
To investigate changes in axonal flow after nerve root compression by using immunohistochemical techniques to detect calcitonin gene-related peptide (CGRP), which is thought to be involved in pain sensation.


METHODS
Experimental, controlled study.


METHODS
University medical school in Japan.


METHODS
Forty adult mongrel dogs (weight, 7-15kg).


METHODS
In dogs, the lumbar nerve roots were compressed using 4 types of clips with different pressures. Changes of CGRP levels in the spinal dorsal horn, dorsal root, and dorsal root ganglia (DRG) were examined immunohistochemically after compression for 24 hours or for 1 week.


METHODS
CGRP-positive neurons and CGRP-positive fibers.


RESULTS
After compression, axonal flow in the dorsal root was impaired, accumulation of CGRP was observed distal to the site of compression, and the number of DRG cells showing positively for CGRP decreased. Compression for 1 week resulted in a decrease in the number of CGRP-positive fibers in the spinal dorsal horn.


CONCLUSIONS
These findings indicate that CGRP dynamics are modified by nerve compression.
Kobayashi
Shigeru
S
Department of Rehabilitation Medicine, Fukui University School of Medicine, Matsuoka, Fukui, Japan. kshigeru@fmsrsa.fukui-med.ac.jp
Sasaki
Shinichi
S
Shimada
Seiichiro
S
Kaneyasu
Makoto
M
Mizukami
Yasutaka
Y
Kitade
Ippei
I
Ogawa
Mayumi
M
Kawahara
Hideo
H
Baba
Hisatoshi
H
Yoshizawa
Hidezo
H
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D004285:Dogs; D005727:Ganglia, Spinal / Q000473:pathology; D009475:Neurons, Afferent / Q000473:pathology*; D011843:Radiculopathy / Q000473:pathology*
D015740:Calcitonin Gene-Related Peptide