Capacitative Ca2+ entries and mRNA expression for TRPC1 and TRPC5 channels in human epidermoid carcinoma A431 cells.
In human epidermoid carcinoma A431 cells, capacitative Ca2+ entries in response to intracellular Ca2+ store depletion with thapsigargin, an endoplasmic reticulum Ca(2+)-ATPase inhibitor, and uridine 5'-triphosphate, a phospholipase C-linked agonist, were inhibited by trivalent cations such as Gd3+ and La3+, and by the store-operated Ca2+ channel inhibitor, 2-aminoethoxydiphenyl borate. Of the seven types of canonical transient receptor potential (TRPC) channels as molecular candidates for store-operated Ca2+ channels, mRNAs for TRPC1 and TRPC5 were detected in the cells with the reverse transcription-polymerase chain reaction. Western blotting confirmed the protein expressions of TRPC1 and TRPC5 in A431 cells. The present results suggest that TRPC1 and/or TRPC5 channels serve as store-operated Ca2+ channels in A431 cells, and may function as regulators for intracellular Ca2+ signaling.
Yoshida
Junko
J
Department of Pharmacology, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan. yayuyo@kanazawa-med.ac.jp
Ishibashi
Takaharu
T
Imaizumi
Noriko
N
Takegami
Tsutomu
T
Nishio
Matomo
M
D001483:Base Sequence; D001896:Boron Compounds / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005682:Gadolinium / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D007811:Lanthanum / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050052:TRPC Cation Channels; D019284:Thapsigargin / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology
D001896:Boron Compounds; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D027682:Cation Transport Proteins; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D050052:TRPC Cation Channels; C491780:TRPC5 protein, human; C096351:transient receptor potential cation channel, subfamily C, member 1; D019284:Thapsigargin; D007811:Lanthanum; D005682:Gadolinium; C109986:2-aminoethoxydiphenyl borate; D000252:Calcium-Transporting ATPases; D002118:Calcium; D014544:Uridine Triphosphate