Early protective effects of iloprost, a stable prostacyclin analog, during spinal cord ischemia in a rabbit model.
Spinal cord ischemia may develop into paraplegia in some cases during operation of the thoracoabdominal aorta. This is attributable to the vulnerability of spinal motor neurons to ischemia. In this study, iloprost was used as an agent to decrease the severity of ischemia and reperfusion injury to the spinal cord motor neurons. Twenty-one rabbits were randomized into three groups of seven animals each: group A (iloprost not administered), group B (25 ng/kg per minute iloprost), and group S (sham-operated). The spinal cord ischemia model was created by a 15-min occlusion of the aorta just caudal to the renal artery with a balloon catheter. Administration of iloprost began 10 min before occlusion of the aorta, and continued thereafter for 60 min. The pre- and postocclusion arterial pressure and heart rate recordings, results of blood gas analyses, and hematocrit and glucose levels were recorded. The spinal cords were removed after 8-h monitoring of neurologic function. Viable and nonviable motor neurons in the anterior horn of the spinal cord were counted under light microscopy. Any significant alteration in hemodynamics, blood gases, and other physiologic parameters could not be detected within the groups. Iloprost had a moderately hypotensive effect. Neurologic function in terms of Johnson scoring was significantly better in the iloprost group (P<0.05). The number of viable cells was higher, whereas the number of nonviable cells was lower in iloprost group, when compared with the control group (P<0.05). Higher numbers of viable motor neurons were consistent with the neurological findings. As a result of this study we concluded that iloprost infused during clamping of the aorta mitigates the spinal cord injury due to ischemia and reperfusion, and has a significant protective effect.
Silistreli
Erdem
E
Department of Cardiovascular Surgery, School of Medicine, University of Dokuz Eylul, Izmir, Turkey. silistreli@yahoo.com
Kabakci
Birol
B
Yilmaz
Evren
E
Sonmez
Atac
A
Erdal
Cenk
C
Karabay
Ozalp
O
Catalyurek
Hudai
H
Canda
Serafettin
S
Acikel
Unal
U
D000818:Animals; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D006339:Heart Rate / Q000187:drug effects; D016285:Iloprost / Q000494:pharmacology*; D009046:Motor Neurons / Q000187:drug effects* / Q000473:pathology; D009460:Neurologic Examination; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011817:Rabbits; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D013116:Spinal Cord / Q000187:drug effects* / Q000473:pathology; D020760:Spinal Cord Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
D018696:Neuroprotective Agents; D016285:Iloprost