[A study of the expression of Fas after fluid percussion brain injury in rats].
OBJECTIVE
To study the pathologic diagnosis and the injury time estimation in light closed encephalon injury.


METHODS
Mice were hurt by fluid percussion, and were killed at 15, 30 min, 1, 3 , 6, 12 h, 1, 4, 7, 14 d respectively after injury. The expression of Fas-L in the cerebral cortex, thalamus, and hippocampi was detected by immunohistochemistry and the results were assessed by image analysis system.


RESULTS
It is showed that the expression of Fas-L could be detected in 1 h after injury, and increased significantly in three hours, and it reached apex 12 h after injury, and decreased gradually four days after injury, and returned normal 14 days after injury.


CONCLUSIONS
This research demonstrated that Fas-L mediated apoptosis appeared not only around brain trauma but also in the brain tissue far away from the traumatic area. It indicted that the expression of Fas-L is a useful target for diagnosis of early brain injury; the regularity of Fas-L expression could be used as one of indication to date the time of brain injury.
Pan
Hong-fu
HF
West China College of Basic Medicine and Forensic Medicine of Sichuan University, Chengdu 610041, China.
Wang
Ye
Y
Chen
Xiao-gang
XG
Yang
Jing
J
Li
Leibo
L
Peng
Qiyi
Q
Wang
Wendong
W
Liu
Min
M
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D053222:Fas Ligand Protein; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D048069:Tumor Necrosis Factors / Q000096:biosynthesis*
D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins; D048069:Tumor Necrosis Factors