An indirect quantitative method for the assessment of angiogenesis in human tissues.
We set up an ELISA that measures the concentration of von Willebrand factor (vWF) in human endometrial tissue and found a significant correlation with the mean vessel counts in vWF- and CD31-immunostained tissue sections. This ELISA allows an objective and quantitative evaluation of the vascular state in the endometrium and could be used as a method to evaluate the angiogenic state in other tissues.
Lawson
Christine
C
Laboratoire d'Endocrinologie de la Reproduction, Centre de Recherche, Hôpital Saint-François d'Assise, Université Laval, Québec, Québec, Canada.
Herrmann-Lavoie
Catherine
C
Lemay
André
A
Mourad
Walid
W
Akoum
Ali
A
D004717:Endometrium / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism*
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014841:von Willebrand Factor