Effects of growth hormone on the differentiation of mouse B-lymphoid precursors.
Growth hormone (GH) has been known to enhance immune responses directly or through insulin-like growth factor-I (IGF-I). The present study aimed to clarify the roles of GH in the differentiation of B-lineage precursors. In short-term bone marrow cultures, which contained stem cells and early B-lineage cells, GH (10 mug/L) treatment for one day decreased the percentages of stem cells (0.5-fold) and increased those of B-lineage cells (1.4-fold). Furthermore, GH changed the expressions of transcription factors for B cell progenitors differentiation such as paired box gene-5 (Pax-5), immunoglobulin-associated-alpha (Ig-alpha)/CD79a, Ig-beta/CD79b, and IGF-I. Thus, a physiological concentration of GH stimulated the differentiation of B-lymphoid precursors from bone marrow stem cells. Since mRNAs of both GH and GH receptor were present in stem cells and B-cell precursors in bone marrow, GH may modulate B-lymphoid precursors development in an autocrine or paracrine manner in bone marrows.
Sumita
Kiminobu
K
Department of Pharmacology, Kansai Medical University, Osaka. sumitak@takii.kmu.ac.jp
Hattori
Naoki
N
Inagaki
Chiyoko
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018952:Antigens, CD34 / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000187:drug effects; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D002478:Cells, Cultured; D005434:Flow Cytometry; D005819:Genetic Markers; D013006:Growth Hormone / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010799:Phycoerythrin / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors / Q000187:drug effects
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D005819:Genetic Markers; D012333:RNA, Messenger; D014157:Transcription Factors; D010799:Phycoerythrin; D013006:Growth Hormone