[Convolution spectrometric determination of the effect of hydroxyl radical scavenger on UV radiation-induced DNA aberrance].
Convolution spectrometry was applied to determining the DNA aberrance induced by UV-A, UV-B and UV-C radiation, and the extent of minute aberrance was quantitatively expressed by the differential value of convolution spectra. The differential values of DNA solutions irradiated by UV-A, UV-B and UV-C increase in turn, indicating an increscent DNA aberrance. The addition of dimethylsulfoxide or mannitol to DNA solution decreases the differential value in respective way under UV-A, UV-B and UV-C radiation, representing different inhibitory effects. Dimethylsulfoxide decreases the UV-A-induced DNA aberrance by 60% (approximate value), UV-C-induced aberrance by 35% (approximate value), and UV-B-induced aberrance by 20% (approximate value), while mannitol shows similar inhibitory effects (40%, approximate value) for UV-A, UV-B and UV-C-induced DNA aberrance. Although the extent of DNA aberrance varies, the distribution of differential points of convolution spectra reveals that the types of UV radiation-induced DNA aberrance are similar, and the inhibitory mechanism of two hydroxyl radical scavengers is possibly the same.
Lu
Feng
F
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Xiao
Jie
J
Lin
Pei-ying
PY
Yang
Gen-jin
GJ
D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000633:toxicity*; D008027:Light; D009690:Nucleic Acid Conformation / Q000528:radiation effects*; D013057:Spectrum Analysis; D014466:Ultraviolet Rays / Q000009:adverse effects*
D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D004247:DNA