title: Development of optically pure pyrethroid-like fluorescent substrates for carboxylesterases.
abstract: Pyrethroids are now the world's most extensively used insecticides. One of the common metabolic routes of pyrethroid insecticides in living systems is hydrolysis by carboxylesterases, and this hydrolysis may be stereospecific since most pyrethroid insecticides have chiral centers. In previous studies, pyrethroid-like fluorescent substrates have been shown to be hydrolyzed in a fashion similar to actual pyrethroids. It is important to synthesize the stereoisomers of pyrethroid-like fluorescent substrates to study the stereointeraction between carboxylesterases and these substrates. In this study, an effective synthetic method for preparing optically enriched (R)- and (S)-alpha-2-hydroxy-2-(6-methoxy-2-naphthyl)acetonitrile was developed. With this alcohol, an efficient synthetic route for preparation of optically pure cypermethrin and fenvalerate analogues was provided. Identification of these stereoisomers was determined based on GC, HPLC, 1H NMR, and X-ray crystallography. In addition, stereointeraction between carboxylesterases and chiral fluorescent substrates indicated that (i) stereospecificity of recombinant mouse liver carboxylesterases (NCBI accession nos. BAC36707 and NM_133960) varied significantly (up to 300-fold difference) with different stereoisomers of cypermethrin and fenvalerate analogues; (ii) on the basis of Vmax, the sensitivity of this analytical method, using a single stereoisomer of cypermethrin analogues instead of a mixture of eight stereosiomers, could be enhanced by 4-6 times for detection of these carboxylesterases; and (iii) possible usage of these carboxylesterases for chiral synthesis is discussed.
authors.affiliation: Department of Entomology and Cancer Research Center, University of California, Davis, California 95616, USA.
authors.forename: Huazhang
authors.initials: H
authors.lastname: Huang
authors.forename: Jeanette E
authors.initials: JE
authors.lastname: Stok
authors.forename: Donald W
authors.initials: DW
authors.lastname: Stoutamire
authors.forename: Shirley J
authors.initials: SJ
authors.lastname: Gee
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Hammock
mesh_terms: D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000032:analysis / Q000138:chemical synthesis* / Q000378:metabolism; D007536:Isomerism; D008099:Liver / Q000201:enzymology; D051379:Mice; D015394:Molecular Structure; D011722:Pyrethrins / Q000032:analysis / Q000138:chemical synthesis* / Q000378:metabolism; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
chemical_list: D007306:Insecticides; D011722:Pyrethrins; D002265:Carboxylic Ester Hydrolases