[Molecular recognitions of purines by hematoporphyrin and metalloporphyrin receptors].
The present paper studies the interactions of purines and porphyrin by fluorescence, UV, and NMR spectra. The optimal conditions (acidity and ion intensity) of the interaction of purines and hematoporphyrin and metalloporphyrin were investigated in detail. The result shows that hematoporphyrin and metalloporphyrin exhibit significant recognition of purines. The binding constants of metalloporphyrin: K(Ni(II)-HP) > K(Co(II)-HP) > K(Zn(II)-HP) > K(Cu(II)-HP) in pH 11.2 Kolthoff buffer solution. The mechanism of recognition was further discussed. Recognition is achieved by cooperative functions of three recognition groups (metalloporphyrin) and two recognition groups (hematoporphyrin).
Zhang
Yong
Y
Department of Chemistry, Shanxi University, Taiyuan 030006, China.
Lei
Ya-chun
YC
Pan
Jing-hao
JH
Liu
Dian-sheng
DS
D001665:Binding Sites; D018360:Crystallography, X-Ray; D006415:Hematoporphyrins / Q000737:chemistry*; D007700:Kinetics; D008665:Metalloporphyrins / Q000737:chemistry*; D015394:Molecular Structure; D011487:Protein Conformation; D011687:Purines / Q000378:metabolism*
D006415:Hematoporphyrins; D008665:Metalloporphyrins; D011687:Purines