title: Relationship between single nucleotide polymorphisms in leptin, IL6 and adiponectin genes and their circulating product in morbidly obese subjects before and after gastric banding surgery.
abstract: BACKGROUND
  Certain adipose-produced signals are secreted in proportion to body fat mass and are involved in regulation of the energy metabolism of the whole body. Leptin, IL6 and adiponectin can be considered as adiposity signals. Several Single Nucleotide Polymorphisms (SNPs) in genes encoding for these molecules are known to influence their concentration in situations of stable weight. We hypothesized that polymorphism effects could be better detected in a situation of negative energy balance and that modified concentrations of adiposity signal genes could change the dynamics of weight gain in obese subjects.
  
  
  METHODS
  65 obese patients undergoing gastric banding surgery were genotyped for LEP+19A-->G, LEP-2548G-->C, IL6-174G-->C, APM1-11377C-->G and PM1-11391G-->A common SNPs. BMI and concentrations of leptin, IL6 and adiponectin were measured before surgery and after 1 year.
  
  
  RESULTS
  All SNPs except IL6-174G-->C SNP were associated with modifications of the circulating concentrations of signals produced by adipose tissue at baseline. During weight loss, variant genotype carriers of LEP -2548 and +19 SNPs were characterized by a trend towards less decrease in circulating leptin. Weight loss was associated with an increase in IL6 concentration (16.9%+/-12.2) in the IL6-174 C/C genotype carriers, whereas the C/G or G/G genotypes carriers showed a decrease in IL6 (19.9%+/-5.2, P=0.001).
  
  
  CONCLUSIONS
  We observed that the SNPs studied could modulate the concentration of adiposity signals not only at baseline but also during weight loss. Such variations may be sensed by the homeostatic feedback system that controls energy balance and may in turn contribute to some disturbances in weight regulation.
authors.affiliation: INSERM Avenir EA 3502, Paris 6 University, Nutrition Department, AP/HP, Hôtel-Dieu, Paris, France. christine.poitou@ea3502.org
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Lacorte
authors.forename: Muriel
authors.initials: M
authors.lastname: Coupaye
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bertrais
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Bedel
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lafon
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Bouillot
authors.forename: Pilar
authors.initials: P
authors.lastname: Galan
authors.forename: Françoise
authors.initials: F
authors.lastname: Borson-Chazot
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: Christiane
authors.initials: C
authors.lastname: Coussieu
authors.forename: Karine
authors.initials: K
authors.lastname: Clément
mesh_terms: D052242:Adiponectin; D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D015391:Gastroplasty / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D020738:Leptin / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery*; D020641:Polymorphism, Single Nucleotide; D011184:Postoperative Period; D011300:Preoperative Care; D011336:Probability; D011379:Prognosis; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D016896:Treatment Outcome; D015431:Weight Loss
chemical_list: D052242:Adiponectin; D005819:Genetic Markers; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D020738:Leptin