[RT-PCR detecting NUP98-HOX fusion gene in leukemia].
To investigate whether there are NUP98-HOXA, NUP98-HOXB, NUP98-HOXC, NUP98-HOXD fusion genes in leukemia patients in Xinjiang, cellular total RNA was extracted from the bone marrow mononuclear cells, the formaldehyde-agarose gel electrophoresis was used to judge whether RNA was intact, the 17 RT-PCR primers were designed to amplify the predicted fusion junctions and 412 bp GAPDH was used as an internal control, NUP98-HOXA fusion genes were amplified by nested-PCR following reverse transcription. One-step PCR was performed to amplify the other predicted fusion genes. The results showed that RNA was proved to be intact and expression of GAPDH was found in every sample. However, no predicted fusion transcripts were detected in leukemia patients. In conclusion, no NUP98-HOX fusion genes were detected in the samples from Xinjiang.
Zhang
Yan
Y
Medical Research Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
Li
Ling
L
Wen
Bing-Zhao
BZ
Lin
Ren-Yong
RY
Cao
Xu
X
Wang
Ning
N
Ha Li Da
Ya Seng
YS
Jiang
Ming
M
Wen
Hao
H
Lu
Xiao-Mei
XM
Feng
Xiao-Hui
XH
Wang
Xin
X
D000328:Adult; D001854:Bone Marrow Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic
C470262:NUP98-HOXC13 fusion protein, human; D015514:Oncogene Proteins, Fusion