[Specific humoral immune response in Rhesus monkeys vaccinated immune response with Abeta42 peptide vaccine].
OBJECTIVE
To observe the humoral immuneresponse in Rhesus monkey induced by Abeta42 peptide vaccination.


METHODS
Five male Rhesus monkeys were received intramuscular injection of Abeta42 peptide vaccine at 0, 2nd, 6th, 10th, 14th, 18th, 22th week. The titers and Ig subclasses of the serum anti-Abeta42 antibody were measured by ELISA. The specificity of the anti-Abeta42 antibody was determined by Western blot. The recognition of Abeta plaques in Tg2576-transgenic mouse brain tissue by anti-Abeta42 serum were detected by immunohistochemical staining.


RESULTS
At the 8th week after the vaccination, the serum anti-Abeta42 antibody was detected. The titers of the antibody increased with times of booster inoculation and reached 1:4,320 at the 24th week, then decreased. The produced antibodies were mainly IgG1 and IgG2(IgG2/IgG1>1). The anti-Abeta42 antibody had high specificity. The Abeta plaques in Tg2576-transgenic mouse brain tissue were recognized by the antisera.


CONCLUSIONS
Abeta42 peptide vaccine can induce effectively specific humoral immuneresponse in Rhesus monkey.
Li
Shao-bing
SB
Department of Anatomy, Zhongshan Medical College, SUN Yat-Sen University, Guangzhou 510080, China.
Wang
Hua-qiao
HQ
Lin
Xian
X
Xu
Jie
J
Yao
Zhi-bin
ZB
D000544:Alzheimer Disease / Q000276:immunology; D023582:Alzheimer Vaccines / Q000276:immunology*; D016229:Amyloid beta-Peptides / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D007700:Kinetics; D008253:Macaca mulatta / Q000276:immunology*; D008297:Male; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology*; D014611:Vaccination
D023582:Alzheimer Vaccines; D016229:Amyloid beta-Peptides; D000906:Antibodies; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)