title: Roxarsone and transformation products in chicken manure: determination by capillary electrophoresis-inductively coupled plasma-mass spectrometry.
abstract: The determination of the animal feed additive roxarsone (3-nitro-4-hydroxyphenylarsonic acid) and six of its possible transformation products (arsenite, arsenate, monomethylarsonate, dimethylarsinate, 3-amino-4-hydroxyphenylarsonic acid, and 4-hydroxyphenylarsonic acid) in chicken manure was investigated using capillary electrophoresis-inductively coupled plasma-mass spectrometry (CE-ICP-MS). Initial method development was conducted using ultraviolet (UV) detection for ruggedness and time efficiency. Separation of these seven arsenic species was effected using a 20 mM phosphate buffer at pH 5.7. The CE-ICP-MS limits of detection in terms of As for each of the species was in the low microg.L(-1) range, corresponding to absolute detection limits in the range 20-70 fg As (based on a 23 nL injection). Overall, the method developed in this study provides high selectivity and low limits of detection (1-3 microg.L(-1) or low-ppb, based on As), uses small sample volume (low nL), and produces minimal wastes.
authors.affiliation: U.S. Environmental Protection Agency, National Exposure Research Laboratory, Environmental Sciences Division, Las Vegas, NV 89119, USA. rosal.charlita@epa.gov
authors.forename: Charlita G
authors.initials: CG
authors.lastname: Rosal
authors.forename: Georges-Marie
authors.initials: GM
authors.lastname: Momplaisir
authors.forename: Edward M
authors.initials: EM
authors.lastname: Heithmar
mesh_terms: D000818:Animals; D002645:Chickens; D003049:Coccidiostats / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008372:Manure / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D012406:Roxarsone / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003049:Coccidiostats; D008372:Manure; D012406:Roxarsone