Setagin and secretagogin-R22: Posttranscriptional modification products of the secretagogin gene.
We describe two new variants of the recently identified hexa-EF-hand calcium binding protein secretagogin. The first variant (secretagogin-R22) is characterized by one single amino acid exchange (Q/R) at codon 22, most likely due to RNA editing. The second variant of secretagogin (setagin) consists of 49 amino acids. Due to a frame shift, only the first 27 amino acids are identical to secretagogin. We demonstrate that this protein truncation results in complete loss of the calcium binding capacity. Setagin expression was found in considerable amounts in the pancreas whereas secretagogin and secretagogin-R22 were also found in the central nervous system and organs containing neurendocrine cells.
Zierhut
Barbara
B
Department of Medicine III, University of Vienna, Vienna A-1090, Austria.
Daneva
Teodora
T
Gartner
Wolfgang
W
Brunnmaier
Barbara
B
Mineva
Ivelina
I
Berggård
Tord
T
Wagner
Ludwig
L
D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D064046:Secretagogins; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000235:genetics
D000906:Antibodies; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; C576556:SCGN protein, human; D064046:Secretagogins; D002118:Calcium