Gene expression systems in corynebacteria.
Corynebacterium belongs to a group of gram-positive bacteria having moderate to high G+C content, the other members being Mycobacterium, Nocardia, and Rhodococcus. Considerable information is now available on the plasmids, gene regulatory elements, and gene expression in corynebacteria, especially in soil corynebacteria such as Corynebacterium glutamicum. These bacteria are non-pathogenic and, unlike Bacillus and Streptomyces, are low in proteolytic activity and thus have the potential of becoming attractive systems for expression of heterologous proteins. This review discusses recent advances in our understanding of the organization of various regulatory elements, such as promoters, transcription terminators, and development of vectors for cloning and gene expression.
Srivastava
Preeti
P
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, New Delhi 110 016, India.
Deb
J K
JK
D000192:Actinomycetales / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D012321:DNA-Directed RNA Polymerases; D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
D012321:DNA-Directed RNA Polymerases