[Isolation of classical swine pest virus from homologous and heterologic cell lines].
This study was devoted to the choice of cell line for isolation of CSPV with high sensitivity. For this purpose the homologous transplantable cell lines from the collection of European References Laboratory of CSF (National Veterinary Research Institute, Pulawy, Poland) and heterological primary cell culture from the collection "Biotest-Laboratory". Cell cultures were cultivated as a monolayer in 96-hole microtitration plates. Antigen of CSFV was detected in peroxides-linked assay (PLA). Cell culture SK-6 proved to be the best line for isolation of CSFV from organ samples and samples of infected cell culture. The cell line--SK-6 is recommended for cultivation of CSFV other lines (LT, PK-15) are not fit for this purpose.
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D000818:Animals; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques / Q000662:veterinary*; D013552:Swine