title: Mitochondrial K+ transport and cardiac protection during ischemia/reperfusion.
abstract: Mitochondrial ion transport, oxidative phosphorylation, redox balance, and physical integrity are key factors in tissue survival following potentially damaging conditions such as ischemia/reperfusion. Recent research has demonstrated that pharmacologically activated inner mitochondrial membrane ATP-sensitive K+ channels (mitoK(ATP)) are strongly cardioprotective under these conditions. Furthermore, mitoK(ATP) are physiologically activated during ischemic preconditioning, a procedure which protects against ischemic damage. In this review, we discuss mechanisms by which mitoK(ATP) may be activated during preconditioning and the mitochondrial and cellular consequences of this activation, focusing on end-effects which may promote ischemic protection. These effects include decreased loss of tissue ATP through reverse activity of ATP synthase due to increased mitochondrial matrix volumes and lower transport of adenine nucleotides into the matrix. MitoK(ATP) also decreases the release of mitochondrial reactive oxygen species by promoting mild uncoupling in concert with K+/H+ exchange. Finally, mitoK(ATP) activity may inhibit mitochondrial Ca2+ uptake during ischemia, which, together with decreased reactive oxygen release, can prevent mitochondrial permeability transition, loss of organelle function, and loss of physical integrity. We discuss how mitochondrial redox status, K+ transport, Ca2+ transport, and permeability transitions are interrelated during ischemia/reperfusion and are determinant factors regarding the extent of tissue damage.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: R S
authors.initials: RS
authors.lastname: Carreira
authors.forename: H T F
authors.initials: HT
authors.lastname: Facundo
authors.forename: A J
authors.initials: AJ
authors.lastname: Kowaltowski
mesh_terms: D001692:Biological Transport; D006801:Humans; D019157:Ischemic Preconditioning, Myocardial; D008564:Membrane Potentials / Q000502:physiology; D008929:Mitochondria, Heart / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D018384:Oxidative Stress; D010766:Phosphorylation; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology*
chemical_list: D015221:Potassium Channels; C117780:mitochondrial K(ATP) channel; D011188:Potassium