[Antitumor effect and apoptosis induction in human cancer cell lines by BRM-SJS].
OBJECTIVE
It had been observed that BRM-SJS had antitumor effect in our clinical practice. This study was designed to investigate the antitumor activity of BRM-SJS, and mechanism of its action.


METHODS
In vitro antitumor experiments with MTT method, meanwhile cell morphology, flow cytometry, and agarose gel electrophoresis were performed for determining apoptosis in several tumor cell lines.


RESULTS
BRM-SJS had antitumor effects on human Suzhou human glioma (SHG-44), breast carcinoma (MCF-7), and human pancreas carcinoma (PANC1) in vitro, the IC50 values of BRM-SJS were 0. 299 mg/ml, 1.853 mg/ml and 9.416 mg/ml respectively. At the 2. 5 mg of BRM-SJS on SHG-44 and MCF-7, marked morphological changes, including cell shrinkage and condensation of chromosomes, were observed with electric microscope. The increase of apoptosis in SHG-44 and MCF-7 cells treated with BRM-SJS extracts 0.625 -2.5 mg for 14 -48 h was observed by Annexin-V/PI flow cytometry analysis. Agarose gel electrophoresis of DNA from SHG-44 and MCF-7 cells treated with BRM-SJS extracts 1.25 -5 mg for 24 h or 48 h showed marked DNA Ladder pattern.


CONCLUSIONS
Antitumor activity of BRM-SJS may be related with inducement of apoptosis of tumor cells.
Liu
Zhao-Yang
ZY
Cancer Institute of Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R.China.
Wang
Ze-Guang
ZG
Liu
Xing-Jing
XJ
Tian
Hai-Mei
HM
Surapol
Na
Di-Ya
DY
Zhang
Yi-Fei
YF
Zhang
Wei
W
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology / Q000648:ultrastructure; D002214:Capsules; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005910:Glioma / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000648:ultrastructure; D010946:Plants, Medicinal / Q000737:chemistry; D031363:Scutellaria / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal