title: Bacillus bogoriensis sp. nov., a novel alkaliphilic, halotolerant bacterium isolated from a Kenyan soda lake.
abstract: Strain LBB3(T) isolated from Bogoria soda lake in Kenya is an alkaliphilic, Gram-positive, strictly aerobic, non-motile, spore-forming bacterium. It was identified as a member of the genus Bacillus on the basis of phenotypic and phylogenetic analyses. The organism grows optimally at 37 degrees C and pH 10. The G+C content of the genomic DNA is 37.5 mol%. 16S rRNA gene sequence analysis showed 95 and 96 % sequence similarity with Bacillus pseudofirmus (DSM 8715(T)) and Bacillus alcalophilus (DSM 485(T)), respectively. Furthermore, DNA-DNA hybridization against these two Bacillus species showed 39.0 and 55.5 % similarity, respectively. Based on our observations, strain LBB3(T) is proposed to represent a novel species of the genus Bacillus, for which the name Bacillus bogoriensis sp. nov. is proposed. The type strain of B. bogoriensis is LBB3(T) (=ATCC BAA-922(T)=LMG 22234(T)).
authors.affiliation: Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia.|Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-22 100 Lund, Sweden.
authors.forename: Virginia A
authors.initials: VA
authors.lastname: Vargas
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-22 100 Lund, Sweden.
authors.forename: Osvaldo D
authors.initials: OD
authors.lastname: Delgado
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-22 100 Lund, Sweden.
authors.forename: Rajni
authors.initials: R
authors.lastname: Hatti-Kaul
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-22 100 Lund, Sweden.
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007630:Kenya; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride