Raising antibodies in chickens against primaquine, pyrimethamine, dapsone, tetracycline, and doxycycline.
Antibodies against primaquine, pyrimethamine, dapsone, tetracycline, and doxycycline were raised in chickens inoculated with each drug conjugated to a rabbit albumin carrier. Antibody titres against drug and carrier were highest during week 6 postinoculation. Affinity purified anti-primaquine antibodies did not recognise other drugs, but affinity purified anti-doxycycline and anti-tetracycline antibodies recognised both tetracycline and doxycycline in addition to primaquine. Primaquine was detected in urine from 6 to 12 hours after ingestion of therapeutic doses of the drug by anti-primaquine antibodies in a competitive ELISA. Affinity purified anti-primaquine antibodies detected primaquine in the cytoplasm and localised in organelles in monocytes that had been incubated with therapeutic concentrations of the drug.
Goldring
J P Dean
JP
Department of Biochemistry, University of KwaZulu-Natal (PMB), Scottsville, South Africa. Goldringd@ukzn.ac.za
Thobakgale
Christina
C
Hiltunen
Tamara
T
Coetzer
Theresa H T
TH
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D000962:Antimalarials / Q000276:immunology*; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens / Q000276:immunology*; D003593:Cytoplasm / Q000378:metabolism; D003622:Dapsone / Q000276:immunology; D004318:Doxycycline / Q000276:immunology; D005455:Fluorescent Antibody Technique; D009000:Monocytes / Q000378:metabolism; D011319:Primaquine / Q000276:immunology / Q000378:metabolism / Q000652:urine; D011739:Pyrimethamine / Q000276:immunology; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism; D013752:Tetracycline / Q000276:immunology
D000906:Antibodies; D000962:Antimalarials; D012709:Serum Albumin; D003622:Dapsone; D013752:Tetracycline; D011319:Primaquine; D004318:Doxycycline; D011739:Pyrimethamine