Experimental gingivitis in type 1 diabetics: a controlled clinical and microbiological study.
OBJECTIVE
To monitor clinical and microbiological changes during experimental gingivitis in type 1 diabetics and non-diabetics.


METHODS
Nine type 1 diabetics with good/moderate metabolic control and nine age-gender matched non-diabetics were recruited. Probing pocket depths in all subjects did not exceed 4 mm and none were affected by attachment loss. According to the original model, an experimental 3-week plaque accumulation resulting in experimental gingivitis development and a subsequent 2-week period of optimal plaque control were staged. Subgingival plaque samples were collected at days 0, 21 and 35 from one site per quadrant, pooled and analysed using checkerboard DNA-DNA hybridization.


RESULTS
Diabetics (mean age 25.6+/-5.8 standard deviation (SD), range 16-35 years) had a mean HbA1c level of 8.1+/-0.7% (SD), while non-diabetics (mean age 24.8+/-5.7 (SD), range 15-36 years) were metabolically controlled (HbA1c< or =6.5%). Between Days 0, 21 and 35, no statistically significant differences in mean plaque and gingival index scores were observed between diabetics and non-diabetics. At days 7 and 21, however, diabetics showed statistically significantly higher percentages of sites with gingival index scores > or =2 compared with non-diabetics. Mean DNA probe counts of the red and orange complex species increased significantly (p<0.05) between days 0 and 21 and decreased significantly (p<0.05) between days 21 and 35 in both groups.


CONCLUSIONS
Both diabetics and non-diabetics react to experimental plaque accumulation with gingival inflammation. Type 1 diabetics, however, develop an earlier and higher inflammatory response to a comparable bacterial challenge.
Salvi
Giovanni E
GE
School of Dental Medicine, University of Berne, Berne, Switzerland. giovanni.salvi@zmk.unibe.ch
Kandylaki
Maria
M
Troendle
Amineh
A
Persson
G Rutger
GR
Lang
Niklaus P
NP
D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000145:classification / Q000254:growth & development; D016022:Case-Control Studies; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000150:complications / Q000382:microbiology; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000150:complications*; D005260:Female; D005500:Follow-Up Studies; D005888:Gingival Pocket / Q000150:complications; D005891:Gingivitis / Q000209:etiology*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D010512:Periodontal Index
D006442:Glycated Hemoglobin