title: [Study on the resonance scattering light spectra of atrazine-RNA and its analytical application].
abstract: The Resonance Light Scattering (RLS) spectra of atrazine with yeast ribonucleic acid (yRNA) had been studied. At pH 1.50, the enhanced RLS of atrazine-yRNA system is characterized by the maximum scattering peaks at 320 nm and the RLS intensity is proportional to the concentration of yRNA. Under optimal conditions, the linear ranges of the calibration were 0.6-5.0 microg x mL(-1), the linear regression equation was I = 25.88 + 140.0 c(yRNA, microg x mL(-1)), r = 0.9975, with the limit of detection 20.7 ng x mL(-1) (3delta). The method was applied to the determination of yRNA in synthetic samples and RNA in salt cress (Thellungiella halophila) sample satisfactorily. Mechanism studies show that there are two interaction modes between atrazine and RNA: electrostatic mode and intercalative mode.
authors.affiliation: College of Science, China Agricultural University, Beijing 100094, China.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Ren
authors.forename: Shu-ren
authors.initials: SR
authors.lastname: Jiang
authors.forename: Zhen-hong
authors.initials: ZH
authors.lastname: Rao
authors.forename: Yan-mei
authors.initials: YM
authors.lastname: Xiong
authors.forename: Chun-rong
authors.initials: CR
authors.lastname: Zhang
authors.forename: Dong-dong
authors.initials: DD
authors.lastname: Wang
mesh_terms: D001280:Atrazine / Q000737:chemistry* / Q000633:toxicity; D002138:Calibration; D005506:Food Contamination; D006540:Herbicides / Q000737:chemistry* / Q000633:toxicity; D008027:Light; D057230:Limit of Detection; D012313:RNA / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012542:Scattering, Radiation; D013057:Spectrum Analysis; D014732:Vibration
chemical_list: D006540:Herbicides; D012313:RNA; D001280:Atrazine