A role for alphabeta T cells in the resistant phase of the Brown Norway rat model of vasculitis.
Administration of mercuric chloride (HgCl(2)) to Brown Norway rats causes Th2 dominated autoimmunity including a caecal vasculitis. Disease peaks 14 days after starting HgCl(2) after which animals immunoregulate spontaneously. In a third phase, if animals are rechallenged with HgCl(2) 6 weeks later they appear resistant, developing only attenuated disease. Previous studies suggested a role for CD8(+) cells as partial mediators of resistance but no groups had studied the role of alphabeta T cells, gammadelta T cells or natural killer (NK) cells in resistance. We used adoptive transfer and in vitro cell depletion to show that alphabeta T cells are also partially responsible for resistance. Donor animals were treated with HgCl(2) or saline and killed 21 days later. Cells from donor spleens were transferred into recipient animals which were challenged with HgCl(2) and killed 14 days later. Test recipients received spleen cells from HgCl(2)-treated donors after in vitro depletion of one subset of cells. Recipients receiving spleen cells from saline-treated donors remained susceptible to HgCl(2)-induced vasculitis; those receiving spleen cells from HgCl(2)-treated donors were resistant. Animals receiving alphabeta T-cell-depleted spleen cells from HgCl(2)-treated donors showed partial reversal of resistance. Our results suggest a role for alphabeta T cells in the resistant phase of the Brown Norway rat model of vasculitis.
Vinen
C S
CS
Department of Renal Medicine, St George's Hospital Medical School, London, UK.
Turner
D R
DR
Oliveira
D B G
DB
D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002429:Cecal Diseases / Q000276:immunology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D007108:Immune Tolerance / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008627:Mercuric Chloride / Q000494:pharmacology; D051381:Rats; D011914:Rats, Inbred BN; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014657:Vasculitis / Q000276:immunology*; D015431:Weight Loss / Q000187:drug effects
D000891:Anti-Infective Agents, Local; D008627:Mercuric Chloride