Cell-based gene therapy experiments in murine experimental autoimmune encephalomyelitis.
With the ultimate goal of developing a novel treatment for multiple sclerosis (MS), we have developed a cell-based gene therapy protocol for the treatment of murine experimental autoimmune encephalomyelitis (EAE), a powerful animal model for MS. We have determined that transduced fibroblasts secreting encephalogenic epitopes, when injected into mice with EAE, cause a striking abrogation of disease. Both myelin basic protein (MBP) and proteolipid protein mini-gene constructs expressed in syngeneic fibroblast cells were capable of ameliorating ongoing EAE induced by MBP protein. These experiments are crucial since they suggest that not all encephalogenic epitopes need be secreted for the control of disease. We also demonstrate the success of this protocol when transduced syngeneic, and most importantly, allogeneic cells are sequestered within an implantable chamber. Furthermore, we find that through modifying antigen expression by changing the signal sequence of the mini-gene construct, we were able to significantly reduce the dose of cells required for treatment. These improvements to the mini-gene delivery system are critical for the eventual translation of our protocol to the clinic.
Louie
K A
KA
Department of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Weiner
L P
LP
Du
J
J
Kochounian
H H
HH
Fling
S P
SP
Wei
W
W
McMillan
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015194:Diffusion Chambers, Culture; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000628:therapy*; D005260:Female; D005347:Fibroblasts / Q000637:transplantation; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000628:therapy; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D011510:Proteolipids / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D014161:Transduction, Genetic
D004676:Myelin Basic Protein; D011510:Proteolipids