Detection and isolation of winter dysentery bovine coronavirus circulated in Korea during 2002-2004.
Although winter dysentery (WD) has been suspected to occur frequently in Korea, to date the exact epidemiology of WD has remained unknown. Therefore, we investigated the causative agents of WD by using electron microscopy, ELISA, RT-PCR, and nested PCR from 97 fecal samples of 32 WD-affected herds collected from 8 provinces during 2002-2004. The bovine coronavirus (BoCV) was consistently detected in all herds with WD. Of other pathogens, only coccidian oocyts were inconsistently but concurrently detected with BoCV. Ten isolates were identified as BoCV by immune electron microscope, immunofluorescent test and ELISA with antiserum to BoCV, and RT-PCR. From these results, it is concluded that WD caused by BoCV occurred in relatively high frequency and was widespread in Korea. The results provide important epidemiological data for the control and establishment of a surveillance system for WD in Korea.
Jeong
Jae-Ho
JH
Veterinary Medical Research Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Kim
Gye-Yeop
GY
Yoon
Soon-Seek
SS
Park
Su-Jin
SJ
Kim
You-Jung
YJ
Sung
Chang-Min
CM
Jang
Oh-Jun
OJ
Shin
Sung-Shik
SS
Koh
Hong-Bum
HB
Lee
Bong-Joo
BJ
Lee
Chai-Yong
CY
Kang
Mun-Il
MI
Kim
Hyun-Jin
HJ
Park
Nam-Yong
NY
Cho
Kyoung-Oh
KO
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary*; D017938:Coronavirus, Bovine / Q000302:isolation & purification* / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007723:Korea / Q000453:epidemiology; D008854:Microscopy, Electron / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary