Inhibition assay of biomolecules based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles.
An inhibition assay method was developed based on the modulation in the FRET efficiency between quantum dots (QDs) and gold nanoparticles (AuNPs) in the presence of the molecules which inhibit the interactions between QD- and AuNP-conjugated biomolecules. For the functionalization, AuNPs were first stabilized by chemisorption of n-alkanethiols and then capped with the first generation polyamidoamine (G1 PAMAM) dendrimers. By employing a streptavidin-biotin couple as a model system, avidin was quantitatively analyzed as an inhibitor by sensing the change in photoluminescence (PL) quenching of SA-QDs by biotin-AuNPs. The detection limit for avidin was about 10 nM. It is anticipated that the PL quenching-based sensing system can be used for the quantitative analysis and high throughput screening of molecules which inhibit the specific biomolecular interactions.
Oh
Eunkeu
E
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejon 305-701, Korea.
Hong
Mi-Young
MY
Lee
Dohoon
D
Nam
Sung-Hun
SH
Yoon
Hyun C
HC
Kim
Hak-Sung
HS
D001360:Avidin / Q000032:analysis; D001710:Biotin / Q000737:chemistry*; D050091:Dendrimers; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D045663:Quantum Dots; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry*
D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D001360:Avidin; D001710:Biotin; D006046:Gold; D019809:Streptavidin