title: Receptor-mediated basic fibroblast growth factor signaling regulates cyclic pressure-induced human endothelial cell proliferation.
abstract: Vascular endothelial cells sense and respond to pressure by molecular mechanism(s) which, to date, remain poorly understood. The present study investigated basic fibroblast growth factor (bFGF) signaling as a putative mechanotransduction pathway involved in the proliferative responses of human umbilical vein endothelia cells (HUVECs) to 60/20 mm Hg cyclic pressure at 1 Hz for 24 h. Under these conditions, the enhanced proliferative response of these HUVECs was not associated with an increased synthesis/release of bFGF, but involved rapid (within 30 min from the onset of exposure to pressure) tyrosine phosphorylation of the bFGF receptor, FGFR-2. Furthermore, monoclonal antibodies to either bFGF or FGFR-2 attenuated the increased proliferation of HUVECs exposed to 60/20 mm Hg cyclic pressure. HUVECs proliferation under 60/20 mm Hg at 1 Hz cyclic pressure is, therefore, dependent upon bFGF and involves FGFR-2 activation.
authors.affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, USA.
authors.forename: Hainsworth Y
authors.initials: HY
authors.lastname: Shin
authors.forename: Eric A
authors.initials: EA
authors.lastname: Schwartz
authors.forename: Rena
authors.initials: R
authors.lastname: Bizios
authors.forename: Mary E
authors.initials: ME
authors.lastname: Gerritsen
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002455:Cell Division / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D010766:Phosphorylation; D017468:Receptors, Fibroblast Growth Factor / Q000276:immunology / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D014443:Tyrosine