title: [Expression of survivin in activated T cells and its significance].
abstract: OBJECTIVE
  To investigate the expression of survivin in activated T cells in vitro and in vivo.
  
  
  METHODS
  Expressions of survivin and proliferating cell nuclear antigen (PCNA) in splenocytes stimulated with ConA or alloantigen were detected by immunohistochemical straining. Flow cytometry was used to analyze CD3, CD25 and survivin expressions and apoptosis of cultured splenocytes. Splenocytes from C57BL/6(H-2b) mice were infused into BALB/c(H-2d) nude mice to observe the survivin expression in vivo after alloantigen stimulation.
  
  
  RESULTS
  Expressions of survivin and PCNA were detected in stimulated cells. Survivin+ cells were CD3+ T cells, and lymphoblasts could simultaneously express CD3, CD25 and survivin. However, not all survivin+ cells expressed CD25. Five days after culture, apoptotic rate of ConA-stimulated splenocytes increased markedly (P<0.001). From day 4 to 7 after infusion, the alloantigen-activated T cell infiltrating in livers of nude mice expressed survivin.
  
  
  CONCLUSIONS
  Survivin can be detected in T cells after in vivo and in vitro stimulation, and thus may be used as a marker of activated T cells. Continuous expression of survivin in T cells needs continuous stimulation with alloantigen in vivo.
authors.affiliation: Key Laboratory of Organ Transplantation Ministry of Education, Institute of Organ Transplantation Tongji Hospital, Tongji Medical College Huazhong University of Sciences and Technology, Wuhan 430030, China.
authors.forename: Bi-cheng
authors.initials: BC
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Sheng
authors.initials: S
authors.lastname: Chang
authors.forename: Dun-feng
authors.initials: DF
authors.lastname: Du
authors.forename: Zhong-hua
authors.initials: ZH
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002453:Cell Cycle / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007700:Kinetics; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D013154:Spleen / Q000166:cytology; D000077022:Survivin; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D013997:Time Factors
chemical_list: C117749:Birc5 protein, mouse; D017252:CD3 Complex; D051034:Inhibitor of Apoptosis Proteins; D053645:Interleukin-2 Receptor alpha Subunit; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D000077022:Survivin