title: [Extracellular-superoxide dismutase production in mesangial cell growing in extracellular matrix].
abstract: To study the protective function against oxygen radicals in the mesangial area, we assessed extracellular superoxide dismutase (EC-SOD) production in mesangial cells (MCs) in vitro. These cells have a major protective function against oxygen radicals in the extracellular space. In two different kinds of culture conditions: "growth medium" with fetal cow serum, and "differentiation medium" with reduced growth factor, and four extracellular matrixes; type I collagen, type IV collagen, laminin and fibronectin, were added to the MC culture. With the difference in the culture media, differentiation medium induced EC-SOD hyper-production associated with the both of the slowing down of cell proliferation and the suppression of IL-6 and IL-8 production. With difference in the extracellular matrix, the presence of type VI collagen and laminin promoted higher production of EC-SOD than fibronectin and type I collagen. Type IV collagen and laminin associated with the physiological condition of the glomeruli promoted EC-SOD production compared with the presence of type I collagen and fibronectin dominantly located in pathological condition. Suppression of EC-SOD production in growth medium along with MC proliferation and chemokine hyper-production compared with production in differentiation medium might mimic reduction of the protective capacity against oxygen radical toxity during mesangial proliferation in the glomerular nephritis. MC proliferation with type I collagen and fibronectin might enhance oxygen radical toxity in the glomeruli, and accelerate glomerular sclerosis through the suppression of EC-SOD production.
authors.affiliation: Nephrology and Rheumatology Division of Internal Medicine, Aichi Medical University, Aichi, Japan.
authors.forename: Harutaka
authors.initials: H
authors.lastname: Yamada
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Adachi
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yamada
authors.forename: Sachiko
authors.initials: S
authors.lastname: Misao
authors.forename: Keisuke
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Wataru
authors.initials: W
authors.lastname: Kitagawa
authors.forename: Naoto
authors.initials: N
authors.lastname: Miura
authors.forename: Cyouryu
authors.initials: C
authors.lastname: You
authors.forename: Masahito
authors.initials: M
authors.lastname: Sakuma
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Arao
authors.initials: A
authors.lastname: Futenma
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Imai
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D018925:Chemokines / Q000032:analysis; D003470:Culture Media; D005109:Extracellular Matrix / Q000201:enzymology*; D005920:Glomerular Mesangium / Q000166:cytology* / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000096:biosynthesis*
chemical_list: D018925:Chemokines; D003470:Culture Media; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase