Proteomic applications of surface plasmon resonance biosensors: analysis of protein arrays.
Proteomics is one of the most important issues in the post-genomic area, because it can greatly contribute to identifying protein biomarkers for disease diagnosis and drug screening. Protein array is a key technology for proteome researches and has been analyzed by various methods including fluorescence, mass spectrometry, atomic force microscopy and surface plasmon resonance (SPR). SPR biosensor is a promising technology in proteomics, since it has various advantages including real-time measurement of biomolecular interactions without labeling and the simple optical system for the device. SPR biosensors have a strong potential for analyzing proteomes by SPR imaging and SPR spectroscopic imaging, even though the challenge is to produce proteins on a proteomic scale.
Yuk
Jong Seol
JS
Department of Molecular and Cellular Biochemistry, Nano-bio Sensor Research Center, Kangwon National University School of Medicine, Chuncheon, Gangwon-do 200-701, South Korea.
Ha
Kwon-Soo
KS
D000818:Animals; D015374:Biosensing Techniques; D006801:Humans; D040081:Protein Array Analysis; D040901:Proteomics; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods