Serial analysis of gene expression in mice with lipopolysaccharide-induced acute lung injury.
OBJECTIVE
To monitor the systemic gene expression profile in a murine model of lipopolysaccharide-induced acute lung injury.


METHODS
Acute lung injury was induced by intratracheal injection of lipopolysaccharide in 3 mice. Another 3 normal mice receiving same volume of normal saline were taken as the controls. The comprehensive gene expression profile was monitored by the recently modified long serial analysis of gene expression.


RESULTS
A total of 24,670 tags representing 12,168 transcripts in the control mice and 26,378 tags representing 13,397 transcripts in the mice with lung injury were identified respectively. There were 11 transcripts increasing and 7 transcripts decreasing more than 10 folds in the lipopolysaccharide-treated mice. The most overexpressed genes in the mice with lung injury included serum amyloid A3, metallothionein 2, lipocalin 2, cyclin-dependent kinase inhibitor 1A, lactate dehydrogenase 1, melatonin receptor, S100 calcium-binding protein A9, natriuretic peptide precursor, etc. Mitogen activated protein kinase 3, serum albumin, complement component 1 inhibitor, and ATP synthase were underexpressed in the lung injury mice.


CONCLUSIONS
Serial analysis of gene expression provides a molecular characteristic of acute lung injury.
Sun
Hai-chen
HC
Center of Emergency Medicine, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China. sun_haichen@yahoo.com.cn
Qian
Xiao-ming
XM
Nie
Shi-nan
SN
Wu
Xue-hao
XH
D000818:Animals; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors / Q000097:blood; D004268:DNA-Binding Proteins / Q000097:blood; D004195:Disease Models, Animal; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D008070:Lipopolysaccharides; D008297:Male; D008668:Metallothionein / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000097:blood; D009687:Nuclear Proteins / Q000097:blood; D000090243:Nucleophosmin; D017510:Protein Folding; D012016:Reference Values; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000235:genetics*; D009418:S100 Proteins / Q000097:blood; D000685:Serum Amyloid A Protein / Q000378:metabolism
D004268:DNA-Binding Proteins; D008070:Lipopolysaccharides; C491054:Mt2 protein, mouse; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C491644:Nupr1 protein, mouse; D009418:S100 Proteins; C076498:Saa3 protein, mouse; D000685:Serum Amyloid A Protein; D000090243:Nucleophosmin; D008668:Metallothionein; D018844:Cyclin-Dependent Kinases