Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV.
A HPLC-UV method for the quantification of six major isoflavonoids, calycosin 7-O-beta-D-glucoside (1), formononetin 7-O-beta-D-glucoside (2), (6alphaR, 11alphaR) 3-hydroxy-9,10-dimethoxypterocarpan-3-O-beta-D-glucoside (3), 7,2'-dihydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside (4), calycosin (5) and formononetin (6), in Radix Astragali (Huangqi) was developed and validated. The method was proven to be sensitive, specific, accurate and precise, as well as effective and easy.
Wu
Tao
T
Shanghai R&D Center for Standardization of Traditional Chinese Medicines, Shanghai, 201203, China.
Annie Bligh
S W
SW
Gu
Li-hua
LH
Wang
Zheng-tao
ZT
Liu
He-ping
HP
Cheng
Xue-mei
XM
Branford-White
Chris J
CJ
Hu
Zhi-bi
ZB
D027883:Astragalus Plant; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007529:Isoflavones / Q000737:chemistry*; D008517:Phytotherapy; D013056:Spectrophotometry, Ultraviolet
D007529:Isoflavones