Particle separation using ultrasound can be used with human shed mediastinal blood.
BACKGROUND
Shed mediastinal blood collected by cardiotomy suction has been shown to be a large contributor to lipid microemboli ending up in different organs. The aim of this study was to test the separation efficiency on human shed blood of a new separation method developed to meet this demand.


METHODS
Shed mediastinal blood collected from the pericardial cavity of 13 patients undergoing cardiac surgery with cardiopulmonary bypass was collected. The blood was processed in an eight-channel parallel PARSUS separator, and separation efficiency was determined.


RESULTS
Erythrocyte recovery, in terms of a separation ratio, varied between 68% and 91%. Minor electrolyte changes took place, where levels of sodium increased and levels of potassium and calcium decreased.


CONCLUSIONS
This study demonstrates that PARSUS technology can be used on human shed mediastinal blood with good separation efficiency. The technology is, thereby, suggested to have future clinical relevance.
Jönsson
Henrik
H
Department of Cardiothoracic Surgery, Center for Heart and Lung Disease, Lund University Hospital, Lund, Sweden. henrik.jonsson@thorax.lu.se
Nilsson
Andreas
A
Petersson
Filip
F
Allers
Mats
M
Laurell
Thomas
T
D001804:Blood Transfusion, Autologous / Q000009:adverse effects / Q000295:instrumentation* / Q000379:methods; D006348:Cardiac Surgical Procedures; D002315:Cardiopulmonary Bypass / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004573:Electrolytes / Q000097:blood; D004617:Embolism / Q000209:etiology / Q000517:prevention & control; D004912:Erythrocytes; D005240:Feasibility Studies; D006400:Hematocrit; D006440:Hemofiltration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008055:Lipids; D008482:Mediastinum / Q000601:surgery*; D046210:Microfluidic Analytical Techniques; D010316:Particle Size; D014465:Ultrasonics
D004573:Electrolytes; D008055:Lipids