Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.).
Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a search of rice sequence databases. Profile analyses revealed that the cloned genes are expressed in unique patterns in various organs. For example, transcripts of OsCIN1, OsCIN2, OsCIN4, and OsCIN7 were detected in immature seeds whereas OsCIN3 gene expression was flower-specific. Further transcript analysis of these genes expressed in developing seeds indicated that OsCIN1, OsCIN2, and OsCIN7 might play an important role involving sucrose partitioning to the embryo and endosperm. Sucrose, a substrate of CINs, induced the accumulation of OsCIN1 transcripts in excised leaves and OsCIN2 in immature seeds, while the level of OsCIN5 was significantly down-regulated in excised leaves treated with sucrose. Infecting the tissues with rice blast (Magnaporthe grisea) as a biotic stressor increased the expression of OsCIN1, OsCIN4, and OsCIN5, suggesting that these genes may participate in a switch in metabolism to resist pathogen invasion. These results demonstrate that OsCIN genes play diverse roles involving the regulation of metabolism, growth, development, and stress responses.
Cho
Jung-Il
JI
Plant Metabolism Research Centre & Graduate School of Biotechnology, Kyung Hee University, Suwon 449-701, Korea. birdlife@khu.ac.kr
Lee
Sang-Kyu
SK
Ko
Seho
S
Kim
He-Kyung
HK
Jun
Sung-Hoon
SH
Lee
Youn-Hyung
YH
Bhoo
Seong Hee
SH
Lee
Kwang-Woong
KW
An
Gynheung
G
Hahn
Tae-Ryong
TR
Jeon
Jong-Seong
JS
D002473:Cell Wall / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D030562:Databases, Protein; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D035264:Flowers / Q000201:enzymology / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D013395:Sucrose; D043324:beta-Fructofuranosidase