Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture-induced rearrangements and interspecies polymorphism.
An analysis of 18S-25S and 5S rRNA genes in intact plants and cultured tissues of some Rauwolfia species was performed to compare these sequences variability occurred as a result of the species evolution in nature and that induced by tissue culture. The restriction fragment length polymorphism of 18S-25S and 5S rDNA was found both in intact plants of various Rauwolfia species and in long-term Rauwolfia serpentina tissue cultures. In addition, changes in the amount of 18S-25S rRNA genes were observed in long-term R. serpentina tissue cultures. The results demonstrate that rDNA variability observed in intact plants as well as in long-term cultures is attributed to differences in the same regions of ribosomal RNA genes.
Andreev
I O
IO
Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, Academy Zabolotnogo Street, 150, Kiev-143, 03143 Ukraine. i.o.andreev@imbg.org.ua
Spiridonova
K V
KV
Solovyan
V T
VT
Kunakh
V A
VA
D004275:DNA, Ribosomal / Q000235:genetics; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015321:Gene Rearrangement / Q000235:genetics*; D017343:Genes, Plant; D020459:Genes, rRNA; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D011926:Rauwolfia / Q000235:genetics*; D013045:Species Specificity; D046509:Tissue Culture Techniques
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S; C065039:RNA, ribosomal, 25S; D015245:Deoxyribonuclease BamHI