Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17.
Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
Kim
Dockyu
D
Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Republic of Korea.
Chae
Jong-Chan
JC
Zylstra
Gerben J
GJ
Sohn
Ho-Yong
HY
Kwon
Gi-Seok
GS
Kim
Eungbin
E
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001673:Biodegradation, Environmental; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D012240:Rhodococcus / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics; D014992:Xylenes / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014992:Xylenes; C026114:2-xylene