Peptide 19 in the rat vagal and glossopharyngeal sensory ganglia.
Peptide 19 (PEP 19) is a 7.6-kDa polypeptide which binds to calmodulin and inhibits calcium-calmodulin signaling. In this study, PEP 19-immunoreactivity (PEP 19-IR) was examined in the rat vagal and glossopharyngeal sensory ganglia. Twenty-nine percent, 59%, and 41% of sensory neurons contained PEP 19-IR in the jugular, petrosal, and nodose ganglia, respectively. These neurons were of various sizes (jugular, mean +/- SD = 635.8 +/- 392.6 microm2, range = 105.9-1695.9 microm2; petrosal, mean +/- SD = 370.9 +/- 228.5 microm2, range = 57.7-1662.7 microm2; nodose, mean +/- SD = 380.5 +/- 157 microm2, range = 87.5-950.4 microm2) and scattered throughout these ganglia. Double immunofluorescence method revealed that PEP 19-IR neurons which had parvalbumin-IR were rare in the ganglia (jugular, 4%; petrosal, 10%; nodose, 8%). PEP 19-IR neurons which contained calbindin D-28k were abundant in the petrosal (20%) and nodose (22%) ganglia but not in the jugular ganglion (8%). Retrograde tracing method indicated that many PEP 19-IR neurons projected to the circumvallate papilla and soft palate. In the soft palate, taste buds were innervated by PEP 19-IR nerve fibers. The present study suggests that PEP 19-IR neurons include chemoreceptors in the vagal and glossopharyngeal sensory ganglia.
Ichikawa
H
H
Department of Oral Function and Anatomy and Biodental Research Center, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. hiroichi@md.okayama-u.ac.jp
Sugimoto
T
T
D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002628:Chemoreceptor Cells / Q000166:cytology / Q000378:metabolism; D017950:Ganglia, Sensory / Q000166:cytology / Q000378:metabolism*; D005930:Glossopharyngeal Nerve / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009620:Nodose Ganglion / Q000166:cytology / Q000378:metabolism; D010160:Palate, Soft / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D010455:Peptides / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism; D014059:Tongue / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D014630:Vagus Nerve / Q000166:cytology / Q000378:metabolism*
D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D010455:Peptides; C119195:Edf1 protein, rat