title: KLK5 and KLK7, two members of the human tissue kallikrein family, are differentially expressed in lung cancer.
abstract: Emerging data indicate that serine proteases of the kallikrein family (KLK) are implicated in various human diseases, including carcinoma; however, kallikrein gene expression has never been investigated in lung cancer. Using RT-PCR and Western blotting, we demonstrated the expression of both KLK5 and KLK7, and their respective proteins (hK5 and hK7) in tumoral and nontumoral lung tissues. Quantitative gene expression was then analyzed in a cohort of 56 patients with non-small cell lung cancer by real-time RT-PCR. KLK5 expression is significantly more expressed in squamous cell carcinoma than in matched nonmalignant lung tissue (P=0.02), whereas expression of KLK7 was decreased in adenocarcinoma (P=0.003). Multivariate analysis revealed diverse correlations between the KLK5 and KLK7 expression levels in nonmalignant and malignant tissues, and clinical parameters, including histotype, metastatic status, and grade. Our findings provide new insight into kallikrein gene expression in hormone-independent carcinoma. Altogether, our results suggest that variability in KLK5 and KLK7 gene expression might be involved in lung tumorigenesis and useful for clinical purposes.
authors.affiliation: INSERM, U618, Protéases et Vectorisation Pulmonaires, Université François Rabelais, Tours 37032, France.
authors.forename: Chris
authors.initials: C
authors.lastname: Planque
authors.forename: Michèle
authors.initials: M
authors.lastname: de Monte
authors.forename: Serge
authors.initials: S
authors.lastname: Guyetant
authors.forename: Jérôme
authors.initials: J
authors.lastname: Rollin
authors.forename: Claire
authors.initials: C
authors.lastname: Desmazes
authors.forename: Valentine
authors.initials: V
authors.lastname: Panel
authors.forename: Etienne
authors.initials: E
authors.lastname: Lemarié
authors.forename: Yves
authors.initials: Y
authors.lastname: Courty
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007610:Kallikreins; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C400147:KLK5 protein, human; C082472:KLK7 protein, human; D007610:Kallikreins; D012697:Serine Endopeptidases