[High cell density fed-batch culture of E.coli expressing rhVEGF121].
OBJECTIVE
To investigate the optimal high cell density fermentation conditions of recombinant E.coli BL21/pET- 24a/hVEGF(121) expressing recombinant human vascular endothelial growth factor (rhVEGF(121)).


METHODS
The effects of the composition of the fermentation medium, induction time and fed-batch carbon sources on the expression level of rhVEGF(121) and cell output were analyzed.


CONCLUSIONS
When cultured in modified M9 medium and induced for 4 h in the presence of 0.5 mmol/L IPTG at 37 degrees celsius; with glycerol as the carbon sources by continuous fed-batch mode, the recombinant E.coli expressed rhVEGF(121) at the level up to 23% of the total proteins and the yield reached 68 g/L. The optimized fermentation condition for recombinant E.coli enables high expression level of rhVEGF(121).
Hu
Zhi-Ming
ZM
Institute of Molecular Immunology, Southern Medical University, Guangzhou 510515, China. hzm@fimmu.com
Ma
Li
L
Zhou
Ming-Qian
MQ
Meng
Min-Jie
MJ
Yang
Jie-Zuan
JZ
Wang
Xiao-Ning
XN
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A