[A study on relationship between rat brain tissues hurts of the gas explosion and the expression of protein kinase CamRNA and c-fos gene.].
OBJECTIVE
To explore relationship between rat brain tissues hurts of gas explosion and the expression of Protein Kinase C alpha mRNA.


METHODS
Build up rat hurt model of gas explosion. In Situ Hybridization (IDH) technique was used to test Protein Kinase C alpha mRNA. Immunohistochemical Assays (IHA) was used to determine c-fos gene protein.


RESULTS
Only a little a mount expression of PKC alpha mRNA and c-fos of the control group was detected. The expression of the cerebral cortex and hippocampus of PKC alpha mRNA 24 h, 48 h and the 48 h increased obviously, and the 48 h reached the peak of expression; (t = 4.12 P < 0.01). The expression of c-fos protein of the cerebral cortex and hippocampus started to increase obviously at 0.5 h and the 4 h reached the peak, then the strength lowered gradually and the expression level came back normal level on fifth day.


CONCLUSIONS
The anoxia of brain tissues due to the gas explosion may promote the expression of PKCamRNA, and PKCamRNA could regulate the expression of the gene of c-fos. Both PKCamRNA and the gene of c-fos are involved in harmful processes to the nerve cells.
Wang
Gui-Zhi
GZ
Hospital of Pingdingshan coal industry group, Henan 467000, China.
Ren
Wen-Jie
WJ
Qin
Zhu-Xuan
ZX
Wang
Xiao-Min
XM
Wu
Song-Yuan
SY
Liu
Ya
Y
Liu
Lin-Hong
LH
Liu
Liang-Ming
LM
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D004195:Disease Models, Animal; D005107:Explosions; D000860:Hypoxia / Q000378:metabolism; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013665:Tear Gases
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D013665:Tear Gases; D051571:Protein Kinase C-alpha