A mitotic septin scaffold required for Mammalian chromosome congression and segregation.
Coordination of cytokinesis with chromosome congression and segregation is critical for proper cell division, but the mechanism is unknown. Here, septins, a conserved family of polymerizing guanosine triphosphate-binding proteins, localized to the metaphase plate during mitosis. Septin depletion resulted in chromosome loss from the metaphase plate, lack of chromosome segregation and spindle elongation, and incomplete cytokinesis upon delayed mitotic exit. These defects correlated with loss of the mitotic motor and the checkpoint regulator centromere-associated protein E (CENP-E) from the kinetochores of congressing chromosomes. Mammalian septins may thus form a mitotic scaffold for CENP-E and other effectors to coordinate cytokinesis with chromosome congression and segregation.
Spiliotis
Elias T
ET
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305-5435, USA.
Kinoshita
Makoto
M
Nelson
W James
WJ
D000818:Animals; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation; D002877:Chromosomes, Human / Q000502:physiology* / Q000648:ultrastructure; D033481:Chromosomes, Mammalian / Q000502:physiology* / Q000648:ultrastructure; D048749:Cytokinesis; D003598:Cytoskeletal Proteins; D004285:Dogs; D019204:GTP-Binding Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D018386:Kinetochores / Q000378:metabolism; D008677:Metaphase; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D010744:Phosphoric Monoester Hydrolases / Q000276:immunology / Q000378:metabolism*; D034622:RNA Interference; D058112:Septins; D008941:Spindle Apparatus / Q000378:metabolism / Q000648:ultrastructure
D002868:Chromosomal Proteins, Non-Histone; D003598:Cytoskeletal Proteins; C091489:KIF2C protein, human; C068688:centromere protein E; D010744:Phosphoric Monoester Hydrolases; D019204:GTP-Binding Proteins; C436485:SEPTIN6 protein, human; D058112:Septins; D016547:Kinesins