title: Intimate relationship between the genes of two transcriptional coactivators, ADA2a and PIMT, of Drosophila.
abstract: PIMT, a transcriptional coactivator which interacts with and enhances nuclear receptor coactivator PRIP function, was identified recently in mammalian cells and suggested to function as a link between two major multiprotein complexes anchored by CBP/p300 and PBP. Here we describe that the gene of the Drosophila homologue of PIMT, designated as Dtl, is closely associated and has an overlapping promoter with a gene encoding another transcriptional coactivator, ADA2a, which in turn participates in GCN5 HAT-containing complexes. Ada2a also produces an RNA polII subunit, RPB4, via alternative splicing; consequently, an overlapping regulatory region serves for the production of three proteins, each involved in transcription. By studying expression of reporter gene fusions in tissue culture cells and transgenic animals we have demonstrated that the regulatory regions of Ada2a/Rpb4 and Dtl overlap and the Dtl promoter is partly within the Ada2a/Rpb4 coding region. The shared regulatory region contains a DRE element, binding site of DREF, the protein factor involved in the regulation of a number of genes which play a role in DNA replication and cell proliferation. Despite the perfectly symmetrical DRE, DREF seems to have a more decisive role in Ada2a/Rpb4 transcription than in the transcription of Dtl.
authors.affiliation: Institute of Biochemistry, Biological Research Center Temesvari krt.62, Szeged, 6726, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Pápai
authors.forename: Orbán
authors.initials: O
authors.lastname: Komonyi
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Tóth
authors.forename: Tibor
authors.initials: T
authors.lastname: Pankotai
authors.forename: Selen
authors.initials: S
authors.lastname: Muratoglu
authors.forename: Andor
authors.initials: A
authors.lastname: Udvardy
authors.forename: Imre
authors.initials: I
authors.lastname: Boros
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004330:Drosophila / Q000166:cytology / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D023061:Gene Order; D008040:Genetic Linkage; D051548:Histone Acetyltransferases; D007763:Lac Operon / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics; D014162:Transfection; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C470558:Ada2a protein, Drosophila; D029721:Drosophila Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D008156:Luciferases; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D001616:beta-Galactosidase