title: Herpes simplex virus entry mediator associates in infected cells in a complex with viral proteins gD and at least gH.
abstract: We examined herpes simplex virus (HSV)-infected human HEp-2 cells or porcine cells that express herpes virus entry mediator (HVEM) for virus and receptor protein interactions. Antibody to HVEM, or its viral ligand gD, coimmunoprecipitated several similar proteins. A prominent 110-kDa protein that coprecipitated was identified as gH. The HVEM/gD/gH complex was detected with mild or stringent cell lysis conditions. It did not form in cells infected with HSV-1(KOS)Rid1 virus or with null virus lacking gD, gH, or gL. Thus, in cells a complex forms through physical associations of HVEM, gD, and at least gH.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109-0620, USA.
authors.forename: Pilar
authors.initials: P
authors.lastname: Perez-Romero
authors.forename: Aleida
authors.initials: A
authors.lastname: Perez
authors.forename: Althea
authors.initials: A
authors.lastname: Capul
authors.forename: Rebecca
authors.initials: R
authors.lastname: Montgomery
authors.forename: A Oveta
authors.initials: AO
authors.lastname: Fuller
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus / Q000378:metabolism*; D013552:Swine; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; C044200:glycoprotein H, herpes simplex virus type 1