title: Development of a PAN-specific, affinity-purified anti-acetylated lysine antibody for detection, identification, isolation, and intracellular localization of acetylated protein.
abstract: Acetylation on the lysine residue is an important event of posttranslational modification of proteins. In this study, we developed a simple method to produce and to affinity purify the specific anti-acetylated lysine polyclonal antibody, which is useful for the detection, identification, isolation, and intracellular localization of acetylated proteins on the lysine residues. We utilized the chemically acetylated hemocyanin of keyhole limpets (KLH) as an immunogen to raise the immune serum and to isolate the population of the acetylated lysine specific antibody using the immobilized acetylated lysine as immunoaffinity-ligand. The isolated antibody was tested to be useful for ELISA, immunoblotting detection, immunofluorescent localization, and affinity isolation of the acetylated proteins.
authors.affiliation: Department of Medicine, ImmuneChem Pharmaceuticals Inc, Burnaby, British Columbia, Canada.
authors.forename: Liping
authors.initials: L
authors.lastname: Qiang
authors.forename: Hao
authors.initials: H
authors.lastname: Xiao
authors.forename: Eric I
authors.initials: EI
authors.lastname: Campos
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Ho
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000107:Acetylation; D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D000915:Antibody Affinity; D000918:Antibody Specificity; D002417:Cattle; D002846:Chromatography, Affinity; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D006657:Histones / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008239:Lysine / Q000737:chemistry* / Q000276:immunology*; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D006657:Histones; D011506:Proteins; D012710:Serum Albumin, Bovine; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D008239:Lysine