Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip.
OBJECTIVE
In order to obtain lymphogenous metastasis-associated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis potential.


METHODS
Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip(r) MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by bioinformatics.


RESULTS
Out of the 14 500 known genes investigated, 110 (0.8%) were up regulated at least 2(3) fold. Among the total 4 371 ESTs, 17 ESTs (0.4%) (data were not presented) were up regulated at least 2(3) fold. According to the Gene Ontology and TreeView analysis, the 110 genes were further classified into two groups: differential biological process profile and molecular function profile.


CONCLUSIONS
Using high-throughput gene chip method, a large number of genes and their cellular functions about angiogenesis, cell adhesion, signal transduction, cell motility, transport, microtubule-based process, cytoskeleton organization and biogenesis, cell cycle, transcription, chaperone activity, motor activity, protein kinase activity, receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further investigation. Cyclin D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later experiments. ESTs (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic metastasis, which might be used as the diagnostic markers and the therapeutic targets for lymphatic metastasis.
Song
Bo
B
Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China.
Tang
Jian-Wu
JW
Wang
Bo
B
Cui
Xiao-Nan
XN
Hou
Li
L
Sun
Lu
L
Mao
Li-Min
LM
Zhou
Chun-Hui
CH
Du
Yue
Y
Wang
Li-Hui
LH
Wang
Hua-Xin
HX
Zheng
Ren-Shu
RS
Sun
Lei
L
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D008113:Liver Neoplasms / Q000235:genetics*; D008207:Lymphatic Metastasis / Q000235:genetics*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis