[Research on discrimination of 3D fluorescence spectra of phytoplanktons].
Six phytoplankton species belonging to four divisions, which may cause red tide, were selected and cultivated under different temperature (20 degrees C and 15 degrees C) and illumination (7000, 4100 and 1100 Lux) in lab. 3D fluorescence spectra were measured in each growth period. Discrimination of these phytoplanktons' 3D fluorescence spectra was explored using discriminant analyses. The result showed that discriminant analyses based on the principal component analysis of spectra data was an efficient method. Isochrysis galbana, Platymonas helgolanidica and Skeletonema costatuma can be discriminated effectively. Skeletonema costatuma, which is an diatom, can be distinguished from Alexandrium tamarense and Prorocentrum micans which belong to Dinophyta.
Zhang
Qian-qian
QQ
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China.
Lei
Shu-he
SH
Wang
Xiu-lin
XL
Wang
Lei
L
Yu
Ping
P
D017377:Diatoms / Q000737:chemistry / Q000145:classification; D004141:Dinoflagellida / Q000737:chemistry / Q000145:classification; D056890:Eukaryota / Q000737:chemistry / Q000145:classification; D005453:Fluorescence; D057097:Harmful Algal Bloom / Q000502:physiology; D008386:Marine Biology; D008856:Microscopy, Fluorescence / Q000379:methods; D010839:Phytoplankton / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D025341:Principal Component Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*