Hepatoprotective effects of the water extract from adzuki bean hulls on acetaminophen-induced damage in rat liver.
We examined the hepatoprotective effect of water-extract from adzuki bean (Vigna angularis) hulls on acetaminophen (AAP)-induced damage in rat liver. F344/DuCrj rats of 8 weeks of age were fed diets without and with 0.5% AAP or besides it 5% adzuki extract (lyophilized) on a daily basis over a period of 4 wk. At that time, serum aspartate aminotransferase activity in only AAP-treated group was higher than in both control and AAP plus adzuki extract (AAPA)-treated groups, while hepatic glutathione content and hepatic glutathione reductase and catalase activities in the AAP-treated group were lower than in the control group in contrast to the reverse in the AAPA-treated group. Hepatic phosphatidylcholine hydroperoxide and phosphatidylethanolamine hydroperoxide concentrations were higher in the AAP-treated group than in the control group, and were lower in the AAPA-treated group than in the AAP-treated group. Hepatic glutathione peroxidase activity was higher in the AAP-treated group than in the control group, although there was no significant difference between both AAP- and AAPA-treated groups in this respect. These findings suggest that the adzuki extract will serve as a prophylactic against oxidative damage to the liver.
Han
Kyu-Ho
KH
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Fukushima
Michihiro
M
Ohba
Kiyoshi
K
Shimada
Ken-ichiro
K
Sekikawa
Mitsuo
M
Chiji
Hideyuki
H
Lee
Chi-Ho
CH
Nakano
Masuo
M
D000082:Acetaminophen / Q000633:toxicity*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury; D007887:Fabaceae / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D014867:Water
D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010936:Plant Extracts; C057590:phosphatidylcholine hydroperoxide; C027850:phosphatidylethanolamine hydroperoxide; D014867:Water; D000082:Acetaminophen; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D001219:Aspartate Aminotransferases; D005978:Glutathione