[Transient cytosolic free calcium changes in cultured neonatal rat cardiac myocytes in response to advanced glycation end-product treatment].
OBJECTIVE
To investigate the effects of advanced glycation end-products (AGEs) on transient cytosolic free calcium in neonatal rat cardiac myocytes (CMs) cultured in vitro.


METHODS
CMs cultured for 3 to 5 days in vitro were incubated with Ca(2+)-sensitive fluorescent indicator Fluo-3AM with light screening at 37 degrees celsius; with 5% CO(2) for 60 min. Changes of the fluorescence signal of free calcium caused by AGEs were measured under laser scanning confocal microscope (LSCM).


RESULTS
Compared with the control cells, AGEs caused an increase in the concentration of cytosolic free calcium in a dose-dependent manner.


CONCLUSIONS
AGEs may impair neonatal rat CMs by altering cytosolic free calcium concentration.
Shao
Ya-Hui
YH
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Xu
Ding-Li
DL
Zeng
Ping
P
Lai
Wen-Yan
WY
Ren
Hao
H
Jiang
Rong-Yan
RY
D000814:Aniline Compounds; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D007202:Indicators and Reagents; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014966:Xanthenes
D000814:Aniline Compounds; D015220:Calcium Channels; D017127:Glycation End Products, Advanced; D007202:Indicators and Reagents; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium