title: Purifying the masses: integrating prepurification quality control, high-throughput LC/MS purification, and compound plating to feed high-throughput screening.
abstract: In this paper we report using a parallel, four-channel HPLC/MUX/MS purification system, the Purification Factory, to purify thousands of compounds destined for high-throughput screening in a single month. The maximum sample throughput during this 20-workday month was 704 samples/day. Since this purification throughput exceeded the postpurification sample and data handling capabilities provided by commercial solutions, a custom-integrated solution was designed to address these shortcomings. In this paper we detail the key improvements in automation, solvent handling, and sample handling logistics implemented to sustain a mean throughput of 528 samples/day over a multimonth time period.
authors.affiliation: Genomics Institute of the Novartis Research Foundation (GNF), 10675 John Jay Hopkins Drive, San Diego, California 92121, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Isbell
authors.forename: Yingyao
authors.initials: Y
authors.lastname: Zhou
authors.forename: Christina
authors.initials: C
authors.lastname: Guintu
authors.forename: Matthew
authors.initials: M
authors.lastname: Rynd
authors.forename: Shumei
authors.initials: S
authors.lastname: Jiang
authors.forename: Dimitri
authors.initials: D
authors.lastname: Petrov
authors.forename: Ken
authors.initials: K
authors.lastname: Micklash
authors.forename: Jim
authors.initials: J
authors.lastname: Mainquist
authors.forename: Jared
authors.initials: J
authors.lastname: Ek
authors.forename: Jim
authors.initials: J
authors.lastname: Chang
authors.forename: Mark
authors.initials: M
authors.lastname: Weselak
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Backes
authors.forename: Andrew
authors.initials: A
authors.lastname: Brailsford
authors.forename: Darcy
authors.initials: D
authors.lastname: Shave
mesh_terms: D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000302:isolation & purification* / Q000592:standards; D011786:Quality Control; D012371:Robotics
chemical_list: D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations