title: Gene expression profiles in human gastric cancer: expression of maspin correlates with lymph node metastasis.
abstract: To seek for a candidate gene that would regulate tumour progression and metastasis in gastric cancer, we investigated gene expression profiles by using DNA microarray. Tumour tissue and adjacent normal tissue were obtained from 21 patients with gastric cancer and then examined for their gene expression profiles by the Gene Chip Human U95Av2 array, which includes 12 000 human genes and EST sequences. A total of 25 genes were upregulated and two genes were downregulated by at least four-fold in the tumour tissue. In a further analysis according to lymph node metastasis, the expressed levels of maspin, as well as carcinoembryonic antigen and nonspecific crossreacting antigen were significantly higher in tumours with lymph node metastasis than in those without it. Maspin expression in 85 gastric cancer patients was further investigated by using immunohistochemistry. Maspin expression was not observed in normal gastric epithelia without intestinal metaplasia. In contrast, maspin was expressed in 74 of 85 tumour tissues. There was a significant correlation between the incidence of maspin-positive tumour staining and lymph node metastasis. These results suggest that maspin has a potential role for tumour metastasis in gastric cancer.
authors.affiliation: Department of Surgery 1, Fukushima Medical University, Fukushima 960-1295, Japan. mterashi@fmu.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Terashima
authors.forename: C
authors.initials: C
authors.lastname: Maesawa
authors.forename: K
authors.initials: K
authors.lastname: Oyama
authors.forename: S
authors.initials: S
authors.lastname: Ohtani
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: S
authors.initials: S
authors.lastname: Ogasawara
authors.forename: A
authors.initials: A
authors.lastname: Takagane
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: N
authors.initials: N
authors.lastname: Kanzaki
authors.forename: S
authors.initials: S
authors.lastname: Matsuyama
authors.forename: Y
authors.initials: Y
authors.lastname: Hoshino
authors.forename: M
authors.initials: M
authors.lastname: Kogure
authors.forename: M
authors.initials: M
authors.lastname: Gotoh
authors.forename: M
authors.initials: M
authors.lastname: Shirane
authors.forename: K
authors.initials: K
authors.lastname: Mori
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation; D005260:Female; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D015843:Serpins / Q000032:analysis / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C085344:SERPIN-B5; D015843:Serpins