Study of stem cell function using microarray experiments.
DNA Microarrays are used to simultaneously measure the levels of thousands of mRNAs in a sample. We illustrate here that a collection of such measurements in different cell types and states is a sound source of functional predictions, provided the microarray experiments are analogous and the cell samples are appropriately diverse. We have used this approach to study stem cells, whose identity and mechanisms of control are not well understood, generating Affymetrix microarray data from more than 200 samples, including stem cells and their derivatives, from human and mouse. The data can be accessed online (StemBase; http://www.scgp.ca:8080/StemBase/).
Perez-Iratxeta
Carolina
C
Ontario Genomics Innovation Centre, Ottawa Health Research Institute, Molecular Medicine Program, 501 Smyth Road, Ottawa, Canada K1H 8L6.
Palidwor
Gareth
G
Porter
Christopher J
CJ
Sanche
Neal A
NA
Huska
Matthew R
MR
Suomela
Brian P
BP
Muro
Enrique M
EM
Krzyzanowski
Paul M
PM
Hughes
Evan
E
Campbell
Pearl A
PA
Rudnicki
Michael A
MA
Andrade
Miguel A
MA
D000818:Animals; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells / Q000502:physiology*