[1H NMR analysis of the complex formation of aromatic molecules of antibiotic and vitamin in aqueous solution: heteroassociation of actinomycin D and flavin mononucleotide].
The molecular mechanism of the combined action of antibiotic and vitamin was studied by NMR spectroscopy. The heteroassociation of the antitumor antibiotic actinomycin D and flavin mononucleotide was investigated as a function of concentration and temperature by 500 MHz 1H NMR spectroscopy. The equilibrium association constant, the thermodynamic parameters (deltaH, deltaS) of heteroassociation of actinomycin D with flavin mononucleotide, and the limiting values of proton chemical shifts in the heterocomplex were determined from the concentration and temperature dependences of proton chemical shifts of molecules. The most favorable structure of the 1:1 actinomycin D-flavin mononucleotide heteroassociation complex was determined using both the molecular mechanics methods (X-PLOR software) and the limiting values of proton chemical shifts of the molecules. In the calculated structure, the planes of the chromophores of actinomycin D and flavin mononucleotide molecules in the 1:1 heterocomplex are parallel and separated from each other by a distance of about 0.34 nm. At the same time, there is a probability of formation of intermolecular hydrogen bonds in the calculated structure of 1:1 actinomycin D-flavin mononucleotide complex. The analysis of the results obtained suggests that aromatic molecules of vitamins, e.g., flavin mononucleotide, can form energetically favorable heterocomplexes with aromatic antitumor antibiotics in aqueous solution, modulating thereby the efficacy of their medical and biological action.
Veselkov
A N
AN
Evstigneev
M P
MP
Rozvadovskaia
A O
AO
Mukhina
Iu V
IuV
Rybakova
K A
KA
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D003609:Dactinomycin / Q000737:chemistry*; D005486:Flavin Mononucleotide / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D012984:Software; D012996:Solutions; D013816:Thermodynamics; D014815:Vitamins / Q000737:chemistry*; D014867:Water
D000900:Anti-Bacterial Agents; D011522:Protons; D012996:Solutions; D014815:Vitamins; D014867:Water; D003609:Dactinomycin; D005486:Flavin Mononucleotide