Efficient derivation of new human embryonic stem cell lines.
Human embryonic stem (hES) cells, unlike most cells derived from adult or fetal human tissues, represent a potentially unlimited source of various cell types for basic clinical research. To meet the increased demand for characterized hES cell lines, we established and characterized nine new lines obtained from frozen-thawed pronucleus-stage embryos. In addition, we improved the derivation efficiency from inner cell masses (to 47.4%) and optimized culture conditions for undifferentiated hES cells. After these cell lines had been maintained for over a year in vitro, they were characterized comprehensively for expression of markers of undifferentiated hES cells, karyotype, and in vitro/in vivo differentiation capacity. All of the cell lines were pluripotent, and one cell line was trisomic for chromosome 3. Improved culture techniques for hES cells should make them a good source for diverse applications in regenerative medicine, but further investigation is needed of their basic biology.
Kim
Sun Jong
SJ
Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Seoul 157-280, Korea.
Lee
Jeoung Eun
JE
Park
Jong Hyuk
JH
Lee
Jung Bok
JB
Kim
Jin Mee
JM
Yoon
Byung Sun
BS
Song
Ji Min
JM
Roh
Sung Il
SI
Kim
Chul Geun
CG
Yoon
Hyun Soo
HS
D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques / Q000379:methods; D015925:Cryopreservation; D016172:DNA Fingerprinting; D033041:Embryo Research; D005347:Fibroblasts; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D016513:Mice, SCID; D039904:Pluripotent Stem Cells / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D013724:Teratoma / Q000473:pathology; D013736:Testicular Neoplasms / Q000473:pathology