Repopulation of oligodendrocyte progenitor cell depleted tissue in a model of chronic demyelination.
Some, but not all chronically demyelinated MS lesions are depleted of oligodendrocyte progenitor cells (OPCs) suggesting that OPCs are destroyed during the process of demyelination and some factor impedes OPC repopulation of the depleted tissue. The chronically demyelinated axons in MS lie in an astrocytic environment and it has been proposed that this might impede entry of OPCs into such regions. By depleting a short length of spinal cord of its OPCs using 40 Gy of X-irradiation in both normal rats and rats with progressive myelin loss accompanied by an astrocytosis (taiep rats), we investigated whether such changes affect the ability of OPCs to repopulate OPC-depleted tissue. In both taiep and normal rats, the rate of repopulation decreases with age, but no difference was detected in the rate at which OPCs repopulated normally myelinated and chronically demyelinated and astrocytosed tissue. This indicates that, if the astrocytic environment of the taiep CNS is comparable to that found in MS lesions, then the presence of chronically demyelinated axons and astrocytosis in chronic MS lesions does not represent a barrier to repopulation of the tissue by OPCs. However, similar to the situation in the normal adult rodent CNS, the rate of repopulation by endogenous OPCs in aged taiep rats is very slow, approximately 0.2 mm per week.
Foote
A K
AK
Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Blakemore
W F
WF
D000367:Age Factors; D000818:Animals; D003711:Demyelinating Diseases / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005911:Gliosis / Q000473:pathology; D008297:Male; D009103:Multiple Sclerosis / Q000473:pathology; D009836:Oligodendroglia / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D051381:Rats; D013116:Spinal Cord / Q000473:pathology* / Q000528:radiation effects; D013234:Stem Cells / Q000502:physiology*