Abdominal wall repair using a biodegradable scaffold seeded with cells.
OBJECTIVE
The repair of large abdominal wall defects is still a challenge for pediatric surgeons. Synthetic materials, however, may lead to high complication rates. This study was aimed at applying tissue-engineering methods to abdominal wall repair.


METHODS
3T3 mouse fibroblasts were expanded in vitro. In the next step, a biodegradable material--polyglycolic acid (PGA)--was actively seeded with 10(7) cells/scm of PGA scaffold. Culture medium (Dulbecco's Modified Eagle's Medium with 10% fetal bovine serum) was changed every 6 hours after seeding cells on PGA fibers. Under general anaesthesia, C57BL/6J black mice underwent creation of a 2 x 3-cm abdominal wall defect (60%-70% of abdominal surface). The defect was repaired in the experimental group with the fibroblast-seeded PGA scaffold. In the first control group, the defect was covered with acellular PGA, and in the second control group, by skin closure. Animals were killed after 30 days to assess the histologic and gross findings.


RESULTS
No abdominal hernia was found in animals repaired with cell-seeded and acellular scaffolds. All animals with skin closure died within 7 days. In every case, tissue-engineered construct was thicker then in controls. Histologic and gross examination revealed a good neovascularisation in tissue-engineered abdominal walls comparing to the acellular matrix. There was no intensive scar formation between abdominal wall and skin.


CONCLUSIONS
Engineered soft tissue constructs can provide structural replacement of severe and large abdominal wall defects. Tissue engineering in the near future will possibly enter clinical practice.
Drewa
Tomasz
T
Department of Urology, The L. Rydygier Medical University, 85-094 Bydgoszcz, Poland. tomaszdrewa@wp.pl
Galazka
Przemyslaw
P
Prokurat
Andrzej
A
Wolski
Zbigniew
Z
Sir
Jan
J
Wysocka
Katarzyna
K
Czajkowski
Rafa
R
D016475:3T3 Cells; D034861:Abdominal Wall / Q000601:surgery*; D020341:Absorbable Implants; D000818:Animals; D046508:Culture Techniques; D005347:Fibroblasts / Q000637:transplantation*; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D011100:Polyglycolic Acid; D019651:Plastic Surgery Procedures; D023822:Tissue Engineering / Q000379:methods*
D011100:Polyglycolic Acid