Topology of chromosomes 18 and X in human blastomeres from 3- to 4-day-old embryos.
The positions of chromosomes 18 and X fluorescence in situ hybridization signals were analyzed in blastomeres generated from human in vitro fertilization 3- to 4-day-old embryos after preimplantation screening of aneuploidy of chromosomes 13, 16, 18, 21, 22, X, and Y. Fluorescent signal localization compared with a three-dimensional sphere model of random signal distribution revealed significant differences, providing evidence of peripheral localization of chromosome 18 in aneuploid (p=0.0013) and aneuploid/euploid blastomeres (p=0.0011). No differences were found in localization of chromosome 18 in euploid and in chromosome X in euploid and aneuploid blastomeres.
Diblík
Jan
J
Institute of Biology and Medical Genetics, University Hospital Motol, V Uvalu 84, Prague 5 CZ-150 06, Czech Republic.
Macek
Milan
M
Magli
Maria-Cristina
MC
Krejcí
Roman
R
Gianaroli
Luca
L
D000328:Adult; D000782:Aneuploidy; D001755:Blastocyst / Q000648:ultrastructure; D001757:Blastomeres / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure; D002887:Chromosomes, Human, Pair 18; D041321:Chromosomes, Human, X; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019836:Preimplantation Diagnosis