title: Differential expression pattern of C4 bundle sheath expression genes in rice, a C3 plant.
abstract: NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PCK) are specifically expressed in bundle sheath cells (BSCs) in NADP-ME-type and PCK-type C4 plants, respectively. Unlike the high activities of these enzymes in the green leaves of C4 plants, their low activities have been detected in the leaves of C3 plants. In order to elucidate the differences in the gene expression system between C3 and C4 plants, we have produced chimeric constructs with the beta-glucuronidase (GUS) reporter gene under the control of the maize NADP-Me (ZmMe) or Zoysia japonica Pck (ZjPck) promoter and introduced these constructs into rice. In leaves of transgenic rice, the ZmMe promoter directed GUS expression not only in mesophyll cells (MCs) but also in BSCs and vascular cells, whereas the ZjPck promoter directed GUS expression only in BSCs and vascular cells. Neither the ZjPck nor ZmMe promoters induced GUS expression due to light. In rice leaves, the endogenous NADP-Me (OsMe1) was expressed in MCs, BSCs and vascular cells, whereas the rice Pck (OsPck1) was expressed only in BSCs and vascular cells. Taken together, the results obtained from transgenic rice demonstrate that the expression pattern of ZmMe or ZjPck in transgenic rice was reflected by that of its counterpart gene in rice.
authors.affiliation: Faculty of Agriculture, Kagawa University, Miki, Kita, Kagawa, 761-0795 Japan. nomura@ag.kagawa-u.ac.jp
authors.forename: Mika
authors.initials: M
authors.lastname: Nomura
authors.forename: Tomonori
authors.initials: T
authors.lastname: Higuchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ishida
authors.forename: Shozo
authors.initials: S
authors.lastname: Ohta
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Komari
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Imaizumi
authors.forename: Mitsue
authors.initials: M
authors.lastname: Miyao-Tokutomi
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tajima
mesh_terms: D002678:Chimera / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics*; D018520:Plant Shoots / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
chemical_list: D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D005966:Glucuronidase; D010730:Phosphoenolpyruvate Carboxylase