title: DNA diagnosis of single gene disorders in patients of non-European origin: experience from Kuwait.
abstract: OBJECTIVE
  The aim of this study was to establish genetic DNA-diagnostic service in Kuwait.
  
  
  METHODS
  Polymerase chain reaction, restriction fragment length polymorphisms, heteroduplex analysis and DNA sequencing were applied.
  
  
  RESULTS
  Direct testing for common mutations had variable success in Kuwaiti patients with different genetic disorders, and additional mutation analysis was required in many cases. Genetic heterogeneity, mutations of Mediterranean, African and Arabic/Middle Eastern origin, and homozygosity by descent are characteristic of patients from Kuwait.
  
  
  CONCLUSIONS
  More efforts aimed at the identification of mutations underlying genetic disorders in Kuwait as well as in other Gulf countries are warranted. This can be achieved by focusing genetic research in the academic institutions of Gulf countries towards this goal.
authors.affiliation: Kuwait Medical Genetics Center, Sulaibikhat, Kuwait. samilchuk@hotmail.com
authors.forename: E
authors.initials: E
authors.lastname: Samilchuk
mesh_terms: D018912:Arabs / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D004252:DNA Mutational Analysis; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics*; D005820:Genetic Testing; D006801:Humans; D007730:Kuwait; D016133:Polymerase Chain Reaction; D015995:Prevalence
chemical_list: D004247:DNA