title: The development of an algorithm for the mass spectral interpretation of phosphoproteins.
abstract: Extended from the peptide mapping method (Fenyo, D., Curr. Opin. Biotechnol. 2000, 11, 391-395) the proposed algorithm takes the mass information of a precursor ion to reconstruct all possible phosphorylated peptide sequences. The mass spectra of product ions from the corresponding precursor ion is then used and compared to the reconstructed sequences to deduce the most probable phosphoprotein. The proposed algorithm also predicts all possible combinations of phosphopeptides, which may serve as a clue for designing proper phosphorylation experiments to validate the existence of these peptides and the corresponding proteins.
authors.affiliation: Department of Chemical Engineering, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yen-Han
authors.initials: YH
authors.lastname: Lin
mesh_terms: D000465:Algorithms; D030562:Databases, Protein; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D011499:Protein Processing, Post-Translational
chemical_list: D007477:Ions; D010455:Peptides; D010748:Phosphopeptides; D010750:Phosphoproteins