title: A high throughput method for the detection of metalloproteins on a microgram scale.
abstract: Proteins that bind transition metals make up a substantial portion of the proteome, and the identification of a metal cofactor in a protein can greatly facilitate its functional assignment and help place it in the context of known cellular pathways. Existing methods for the detection of metalloproteins generally consume large amounts of protein, require expensive equipment, or are very labor intensive, rendering them unsuitable for use in high throughput proteomic initiatives. Here we present a method for the identification of metalloproteins that contain iron, copper, manganese, cobalt, nickel, and/or zinc that is sensitive, quick, robust, inexpensive, and can be performed with standard laboratory equipment. The assay is based on a combination of chemiluminescence and colorimetric detection methods, it typically consumes only 10 microg of protein, and most common chemical components of protein solutions do not interfere with metal detection. Analysis of 52 protein samples was compared with the results from inductively coupled plasma-atomic emission spectrometry to verify the accuracy and sensitivity of the method. The assay is conducted in a 384-well format and requires about 3 h for completion, including a 2-h wait; so whole proteomes can be assayed for metal content in a matter of days.
authors.affiliation: Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, SE-75124 Uppsala, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Högbom
authors.forename: Ulrika B
authors.initials: UB
authors.lastname: Ericsson
authors.forename: Robert
authors.initials: R
authors.lastname: Lam
authors.forename: M Amin
authors.initials: MA
authors.lastname: Bakali H
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Kuznetsova
authors.forename: Pär
authors.initials: P
authors.lastname: Nordlund
authors.forename: Deborah B
authors.initials: DB
authors.lastname: Zamble
mesh_terms: D003124:Colorimetry; D008163:Luminescent Measurements; D008667:Metalloproteins / Q000032:analysis*; D008670:Metals / Q000032:analysis*; D020543:Proteome; D013054:Spectrophotometry, Atomic
chemical_list: D008667:Metalloproteins; D008670:Metals; D020543:Proteome