[Interaction of proteins from general transcription complex RNA polymerase II with oligoribonucleotides].
We have analyzed an interaction of the general transcription complex RNA polymerase II proteins (RNA polymerase II, factors TBP, TFIIB, TFIIF, TFIIE and TFIIH) S. cerevisiae with the oligoribonucleotides. With the help of method EMSA was shown that labeled 32P labeled oligoribonucleotide 5'-ACUCUCUUCCGCAUCGC-3' (r-17) binds with the proteins and generates three species of the complexes with the three major shifts. All the three species of the complexes are RNA specific because a total RNA S. cerevisiae was a competitor for all three species but the TATA-containing oligodeoxyribonucleotide (500-fold molar excess) was not a competitor for its. Complexes 32P-r-17 with the proteins belonging to the middle shift are the sequence specific because unlabeled r-17 was a competitor for its binding (100-fold molar excess) but unlabeled UA-rich oligoribonucleotide (5'-AUAUUAUGUUCAAAA-3) was not a competitor for this shift (500-fold molar excess). Complexes belonging to the upper shift are RNA specific probably. We think 32P-r-17 interaction with the proteins belonging to the under shift is nonspecific corresponding to a sorbtion of 32P-r-17 on a protein. The data presented demonstrate that oligoribonucleotide and oligodeoxyribonucleotide don't compete for the binding sites on a basal transcription complex proteins.
Drachkova
I A
IA
Lysova
M V
MV
Repkova
M N
MN
Prokuda
O V
OV
Sokolenko
A A
AA
Arshinova
T V
TV
Kobzev
V F
VF
Iamkovoĭ
V I
VI
Savinkova
L K
LK
D024202:Electrophoretic Mobility Shift Assay; D046912:Multiprotein Complexes / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry*; D010761:Phosphorus Radioisotopes; D012319:RNA Polymerase II / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D035181:TATA-Box Binding Protein / Q000737:chemistry*; D019473:Transcription Factors, TFII / Q000737:chemistry*
D046912:Multiprotein Complexes; D009843:Oligoribonucleotides; D010761:Phosphorus Radioisotopes; D035181:TATA-Box Binding Protein; D019473:Transcription Factors, TFII; D012319:RNA Polymerase II