Linkage of Ii-Key segment to gp100(46-58) epitope enhances the production of epitope-specific antibodies.
Linkage of the Ii-Key segment of the Ii protein to MHC class II epitope gp100(46-58) using a polymethylene linker significantly enhances the production of epitope-specific antibodies in HLA-DR4-IE transgenic mice. This enhancement is not restricted by the spacer length in between the Ii-Key and epitope. The use of either IFA or CFA induced only epitope-specific IgG1. In contrast, CpG adjuvant induced both IgG1 and IgG2a isotypes. These results indicate that the Ii-Key hybrid technology is a novel and potent method to increase the immunogenicity of a MHC class II epitope. It can also be used to more efficiently generate epitope-specific antibodies.
Kallinteris
Nikoletta L
NL
Antigen Express, Inc., 100 Barber St., Worcester, MA 01606-2478, USA.
Wu
Shuzhen
S
Lu
Xueqing
X
von Hofe
Eric
E
Humphreys
Robert E
RE
Xu
Minzhen
M
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000944:Antigens, Differentiation, B-Lymphocyte / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D005260:Female; D015804:HLA-DR4 Antigen / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008822:Mice, Transgenic; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage
D000944:Antigens, Differentiation, B-Lymphocyte; C408982:CPG-oligonucleotide; D000939:Epitopes; D015804:HLA-DR4 Antigen; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; C045084:invariant chain