Performance of new clean-up column for the determination of ochratoxin A in cereals and foodstuffs by HPLC-FLD.
The performance of the newly developed Mycosep 229 Ochra and Multisep 229 Ochra clean-up columns for ochratoxin A (OTA) determination was evaluated. OTA was subsequently analysed using RP-HPLC with fluorescence detection. Recoveries for frequently contaminated commodities, like cereals, red wine, raisins and green coffee, were estimated. The recoveries obtained for the Mycosep 229 Ochra column were in the range from 87.9 +/- 12.5% (n = 6) for wheat to 99.4 +/- 2.7% (n = 24) for raisins. For Multisep 229 Ochra, recoveries from 76.5 +/- 8.0% (n = 6) for barley to 86.4 +/- 1.4% (n = 24) for raisins were achieved. Limits of detection for all matrices investigated (maize, wheat, rice, barley, raisins, green coffee beans, red wine) were in the range 0.4-2.4 microg kg(-1). The trueness of the method was tested using a certified reference material.
Buttinger
G
G
Konrad Lorenz Str. 20 A-3430 Tulln Austria.
Fuchs
E
E
Knapp
H
H
Berthiller
F
F
Schuhmacher
R
R
Binder
E-M
EM
Krska
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis / Q000302:isolation & purification; D009793:Ochratoxins / Q000032:analysis / Q000302:isolation & purification*
D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A