title: Low immunogenicity of in vitro-expanded human neural cells despite high MHC expression.
abstract: The ability to expand human neural precursor cells in vitro offers new possibilities for future cell therapies. However, concern over immunologically based rejection of in vitro-expanded human neural cells confounds their use as donor cells. Here, we demonstrate that the expression of human leukocyte antigen (HLA) class I and II molecules, but not the co-stimulatory proteins CD40, CD80 and CD86, substantially increase during expansion of neurospheres. Furthermore, peripheral lymphocytes were unresponsive when co-cultured with in vitro-expanded neural cells. Taken together, these results suggest a low immunogenicity of these cultured human neural cells despite HLA incompatibility and high HLA expression.
authors.affiliation: Neurotec Department, Division of Experimental Geriatrics, Karolinska Institutet, Karolinska University Hospital, KFC, 4th floor, Novum, SE-141 86 Stockholm, Sweden.
authors.forename: Jenny
authors.initials: J
authors.lastname: Odeberg
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Piao
authors.forename: Eva-Britt
authors.initials: EB
authors.lastname: Samuelsson
authors.forename: Scott
authors.initials: S
authors.lastname: Falci
authors.forename: Elisabet
authors.initials: E
authors.lastname: Akesson
mesh_terms: D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D005434:Flow Cytometry / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008213:Lymphocyte Activation / Q000502:physiology; D008285:Major Histocompatibility Complex / Q000502:physiology*; D009474:Neurons / Q000276:immunology*; D011247:Pregnancy; D016548:Prosencephalon / Q000166:cytology* / Q000196:embryology; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors
chemical_list: D015703:Antigens, CD