Estradiol/androgen receptors during aging: microsomal distribution in human benign prostatic hypertrophy.
Estradiol and androgen receptor levels were determined in benign prostatic hypertrophy tissue by means of dextran-coated charcoal method. 80% of the cases contained estrogen receptor, whereas 85% of cases contained the androgen receptor. In 50 year old patients, nuclear estradiol and androgen receptors were 48% and 34% higher than the cytosolic values, respectively, whereas in 70 year old patients, the levels of nuclear estradiol and androgen receptors were 6% and 57% higher than the cytosolic values, respectively. Nuclear androgen receptor in 50 year old patients was 34% higher than the estradiol receptor, and in 70 year old patients, androgen receptor was 23% higher than the estradiol receptor. Cytosolic values of estrogen receptor in 50 year old patients were diminished 50%, whereas in 70 year old patients, the ER value was increased 40% with regard to androgen receptor. On the other hand, estradiol receptor values measured in microsomal fractions, and were higher when compared to androgen receptor levels (mean percentage +/- 50%). These findings suggest that the biological effects due to the subcellular distribution of AR or ER and that their microsomal distribution would constitute a reserve mechanism and activation of receptors for the continued growth of benign prostatic hypertrophy.
Salazar
E L
EL
Medical Research Unit, Social Security Mexican Institute, Mexico, DF. edith_lsalazar@hotmail.com
Calzada
L
L
Escontria
M
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D000375:Aging / Q000502:physiology*; D006801:Humans; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology; D011944:Receptors, Androgen / Q000378:metabolism*; D011959:Receptors, Estradiol / Q000378:metabolism*
D011944:Receptors, Androgen; D011959:Receptors, Estradiol