[Flow injection analysis of trace amounts of doxycycline with inhibited chemiluminescence detection].
A novel flow injection chemiluminescence method has been developed for the determination of doxycycline (DC) based on the inhibition of chemiluminescence reaction of Luminol-KMnO4 by using DC in sodium hydroxide medium. Using different concentrations of KMnO4, a series of good linear regression equations of decreased chemiluminescence intensity and the concentration of DC were obtained with different subsections in the concentration range of 0.005-5.0 microg x mL(-1). The detection limit (3sigma) is 2.0 x 10(-3) microg x mL(-1). The method has been successfully applied to the determination of DC in a pharmaceutical preparation.
Li
Nain-bing
NB
College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China.
Duan
Jian-ping
JP
Chen
Hong-qing
HQ
Chen
Guo-nan
GN
D004318:Doxycycline / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis; D013997:Time Factors
D004364:Pharmaceutical Preparations; D008165:Luminol; D004318:Doxycycline