Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation.
An increase in the level of polycythemia rubra vera -1 (PRV-1) mRNA has been reported in some myeloproliferative disorders. We have studied the effects of PRV-1 on cell proliferation and cell survival. In cell growth assays, the number of heterologous cells expressing PRV-1 increased faster than sham-transfected cells, a difference that was more pronounced in serum-free media. Even after 5 days of exposure to serum-free media, cells expressing PRV-1 continued to proliferate, whereas the control cells ceased to proliferate. We conclude that PRV-1 is a pro-proliferation molecule, and hypothesize that its overexpression may have a role in the pathogenesis of myeloproliferative disorders.
Mnjoyan
Zakar
Z
Li
Jun
J
Afshar-Kharghan
Vahid
V
D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation; D002470:Cell Survival; D006224:Cricetinae; D058851:GPI-Linked Proteins; D006133:Growth Substances / Q000494:pharmacology; D007519:Isoantigens / Q000235:genetics / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D014162:Transfection
C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D006133:Growth Substances; D007519:Isoantigens; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface