title: C-terminus of p47(phox) is required for interaction with leukocyte type rat 12-lipoxygenase.
abstract: In yeast two-hybrid system, rat 12-lipoxygenase (12-LO) bound to complete (390 amino acids) or the N-terminus truncated form of human p47 (phox), but not to the C-terminus truncated form (residues 1-286). When glutathione S-transferase fused human p47(phox) was added to an in vitro 12-LO enzyme activity assay, formation of 12-hydroperoxyeicosatetraenoic acid was reduced significantly compared to the C-terminus truncated form. These results indicate that C-terminus of p47(phox) is important for its interaction to rat 12-LO.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Instituet, Stockholm, Sweden.
authors.forename: Michiharu
authors.initials: M
authors.lastname: Tanabe
authors.forename: Hideki
authors.initials: H
authors.lastname: Kamitani
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Olof
authors.initials: O
authors.lastname: Rådmark
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D019255:NADPH Oxidases; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D001092:Arachidonate 12-Lipoxygenase; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1