Comparative characteristics of three human embryonic stem cell lines.
Human embryonic stem (hES) cells have unique features including unlimited growth capacity, expression of specific markers, normal karyotypes and an ability to differentiate. Many investigators have tried to use hES cells for cell-based therapy, but there is little information about the properties of available hES cell lines. We compared the characteristics of three hES cell lines. The expression of SSEA-1, -3, -4, and APase, was examined by immunocytochemistry, and Oct-4 expression was analyzed by RT-PCR. Differentiation of the hES cells in vitro and in vivo led to the formation of embryoid bodies (EBs) or teratomas. We examined the expression of tissue-specific markers in the differentiated cells by semiquantitative RT-PCR, and the ability of each hES cell line to proliferate was measured by flow cytometry of DNA content and ELISA. The three hES cell lines were similar in morphology, marker expression, and teratoma formation. However there were significant differences (P < 0.05) between the differentiated cells formed by the different cell lines in levels of expression of tissue-specific markers such as renin, kallikrein, Glut-2, beta- and delta-globin, albumin, and alpha1-antitrypsin (alpha1-AT). The hES cell lines also differed in proliferative activity. Our observations should be useful in basic and clinical hES cell research.
Lee
Jung Bok
JB
Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Seoul 157-280, Korea.
Kim
Jin Mee
JM
Kim
Sung Jong
SJ
Park
Jong Hyuk
JH
Hong
Seok Ho
SH
Roh
Sung Il
SI
Kim
Moon Kyoo
MK
Yoon
Hyun Soo
HS
D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004475:Ectoderm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology*; D004707:Endoderm / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D050814:Octamer Transcription Factor-3; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D013724:Teratoma / Q000209:etiology / Q000473:pathology; D013736:Testicular Neoplasms / Q000209:etiology / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis
D015295:Antigens, Tumor-Associated, Carbohydrate; D004268:DNA-Binding Proteins; D006028:Glycosphingolipids; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C494174:Pou5f1 protein, mouse; D055746:Stage-Specific Embryonic Antigens; D014157:Transcription Factors; C052924:stage-specific embryonic antigen-3; C066584:stage-specific embryonic antigen-4