Biosensor signal amplification of vesicles functionalized with glycolipid for colorimetric detection of Escherichia coli.
A supramolecular assembly of phospholipid-polymerized diacetylene vesicles functionalized with glycolipid can provide a molecular recognition function. The Escherichia coli-glycolipid binding event leads to a visible color change from blue to red, readily seen with the naked eye and quantified by absorption spectroscopy. The biosensor signal is amplified through a suitable increase of phospholipid content in the mixed lipid vesicles and pH of aqueous solutions.
Su
Yan-Lei
YL
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, People's Republic of China.
Li
Jin-Ru
JR
Jiang
Long
L
Cao
Jie
J
D000114:Acetylene / Q000031:analogs & derivatives / Q000737:chemistry; D001431:Bacteriological Techniques / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004926:Escherichia coli / Q000302:isolation & purification*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D009844:Oligosaccharides / Q000737:chemistry; D010777:Photochemistry; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry; D053279:Polyynes; D013053:Spectrophotometry; D014466:Ultraviolet Rays
C493048:10, 12-pentacosadiynoic acid; D005231:Fatty Acids, Unsaturated; D006017:Glycolipids; D008081:Liposomes; D009844:Oligosaccharides; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D000114:Acetylene; D008358:Mannose; D004134:Dimyristoylphosphatidylcholine