Separation and characterization of alkyltrimethylbenzene derivatives in diesel exhaust particles (DEP).
In our continuing studies on estrogenic compounds in diesel exhaust particles (DEP), we have reported the systematic separation of alkyldibenzothiophenes from the estrogenic hexane fraction of DEP. In this study, another estrogenic fraction was further isolated and characterized. DEP were roughly fractionated by successive extraction with hexane, benzene, dichloromethane, methanol, 1 M ammonia and 1 M HCl. The hexane extract, which exhibited estrogenic activity, was further fractionated by column chromatography and high performance liquid chromatography (HPLC). From one of the obtained fractions that showed estrogenic activity, three alkyltrimethylbenzenes were identified by gas chromatography-mass spectrometry (GC-MS) and 1H-nuclear magnetic resonance (NMR) spectroscopy.
Taneda
Shinji
S
PM2.5/DEP Research Project, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-0053, Japan. taneda.shinji@nies.go.jp
Mori
Yoki
Y
Sakushima
Akiyo
A
Kamata
Kazuyuki
K
Hayashi
Hideyuki
H
Seki
Koh-ichi
K
Sakata
Masakatsu
M
Yoshino
Shin
S
Yamaki
Kouya
K
Sagai
Masaru
M
Suzuki
Akira K
AK
D001555:Benzene Derivatives / Q000737:chemistry* / Q000494:pharmacology*; D004784:Environmental Monitoring; D004967:Estrogens / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006586:Hexanes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D012997:Solvents / Q000737:chemistry; D001335:Vehicle Emissions / Q000032:analysis*
D001555:Benzene Derivatives; D004967:Estrogens; D006586:Hexanes; D012997:Solvents; D001335:Vehicle Emissions