title: Association of the HLA region with multiple sclerosis as confirmed by a genome screen using >10,000 SNPs on DNA chips.
abstract: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system, with a complex genetic background. Here, we present a genome screen for association in small scale, employing 11,555 single nucleotide polymorphisms (SNPs) on DNA chips for genotyping 100 MS patients stratified for HLA-DR2+ and 100 controls. More than 500 SNPs revealed significant differences between cases and controls before Bonferroni correction. A fraction of these SNPs was reanalysed in two additional cohorts of patients and controls, using high-throughput genotyping methods. A marker on chromosome 6p21.32 (rs2395182) yielded the highest significance level, validating the established HLA-DR association.
authors.affiliation: Department of Human Genetics, Ruhr-University, 44780 Bochum, Germany. rene.goedde@rub.de
authors.forename: René
authors.initials: R
authors.lastname: Gödde
authors.forename: Klaus
authors.initials: K
authors.lastname: Rohde
authors.forename: Christian
authors.initials: C
authors.lastname: Becker
authors.forename: Mahammad R
authors.initials: MR
authors.lastname: Toliat
authors.forename: Patricia
authors.initials: P
authors.lastname: Entz
authors.forename: Anita
authors.initials: A
authors.lastname: Suk
authors.forename: Norbert
authors.initials: N
authors.lastname: Müller
authors.forename: Eckhart
authors.initials: E
authors.lastname: Sindern
authors.forename: Michael
authors.initials: M
authors.lastname: Haupts
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schimrigk
authors.forename: Peter
authors.initials: P
authors.lastname: Nürnberg
authors.forename: Jörg T
authors.initials: JT
authors.lastname: Epplen
mesh_terms: D008040:Genetic Linkage; D005819:Genetic Markers; D005820:Genetic Testing; D016678:Genome; D006684:HLA-DR Antigens / Q000235:genetics*; D015802:HLA-DR2 Antigen / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D009103:Multiple Sclerosis / Q000209:etiology / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: D005819:Genetic Markers; D006684:HLA-DR Antigens; D015802:HLA-DR2 Antigen; D059811:HLA-DRB1 Chains