HISTONE DEACETYLASE19 is involved in jasmonic acid and ethylene signaling of pathogen response in Arabidopsis.
Histone acetylation is modulated through the action of histone acetyltransferases and deacetylases, which play key roles in the regulation of eukaryotic gene expression. Previously, we have identified a yeast histone deacetylase REDUCED POTASSIUM DEPENDENCY3 (RPD3) homolog, HISTONE DEACETYLASE19 (HDA19) (AtRPD3A), in Arabidopsis thaliana. Here, we report further study of the expression and function of HDA19. Analysis of Arabidopsis plants containing the HDA19:beta-glucuronidase fusion gene revealed that HDA19 was expressed throughout the life of the plant and in most plant organs examined. In addition, the expression of HDA19 was induced by wounding, the pathogen Alternaria brassicicola, and the plant hormones jasmonic acid and ethylene. Using green fluorescent protein fusion, we demonstrated that HDA19 accumulated in the nuclei of Arabidopsis cells. Overexpression of HDA19 in 35S:HDA19 plants decreased histone acetylation levels, whereas downregulation of HDA19 in HDA19-RNA interference (RNAi) plants increased histone acetylation levels. In comparison with wild-type plants, 35S:HDA19 transgenic plants had increased expression of ETHYLENE RESPONSE FACTOR1 and were more resistant to the pathogen A. brassicicola. The expression of jasmonic acid and ethylene regulated PATHOGENESIS-RELATED genes, Basic Chitinase and beta-1,3-Glucanase, was upregulated in 35S:HDA19 plants but downregulated in HDA19-RNAi plants. Our studies provide evidence that HDA19 may regulate gene expression involved in jasmonic acid and ethylene signaling of pathogen response in Arabidopsis.
Zhou
Changhe
C
Department of Biology, West Virginia University, Morgantown, West Virginia 26506-6057, USA.
Zhang
Lin
L
Duan
Jun
J
Miki
Brian
B
Wu
Keqiang
K
D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D004268:DNA-Binding Proteins; D015536:Down-Regulation / Q000502:physiology; D005030:Ethylenes / Q000378:metabolism* / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism; D006655:Histone Deacetylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007113:Immunity, Innate / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins; D034622:RNA Interference / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D005030:Ethylenes; D009687:Nuclear Proteins; D054883:Oxylipins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C093680:ethylene-responsive element binding protein; C011006:jasmonic acid; C036216:ethylene; D002688:Chitinases; C489849:BGL2 protein, Arabidopsis; D004693:Glucan Endo-1,3-beta-D-Glucosidase; C507152:HDA19 protein, Arabidopsis; D006655:Histone Deacetylases