Protein layer coating method on metal surface by electrochemical process through genetical introduced tag.
The technology of mercaptide self-assembly has previously been used to coat proteins onto metals, but is not practical for many surfaces. In this study, a short peptidic tag was employed for the electrochemical immobilization of proteins on metals (ECtag). A 6-mer histidine (alpha-amino-1H-imidazole-4-propionic acid) homopeptide was employed as an ECtag ligand, which forms coordinate bonds with Ni2+ and other divalent metal ions. Protein A was chosen as a model protein for the immobilization, and was genetically tagged with an ECtag for immobilization onto a Pt electrode surface through the reduction of ECtag:Ni2+ to ECtag:Ni by the electrode potential.
Haruyama
Tetsuya
T
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Kitakyushu, Fukuoka 808-0196, Japan. haruyama@life.kyutech.ac.jp
Sakai
Tsutomu
T
Matsuno
Kouhei
K
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006639:Histidine / Q000737:chemistry* / Q000235:genetics; D008422:Materials Testing; D008670:Metals / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000235:genetics
D020099:Coated Materials, Biocompatible; C471213:His-His-His-His-His-His; D008670:Metals; D009842:Oligopeptides; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D006639:Histidine