title: Protection against group A streptococcal infection by vaccination with self-adjuvanting lipid core M protein peptides.
abstract: We have investigated the lipid polylysine core peptide (LCP) system as a self-adjuvanting group A streptococcal (GAS) vaccine delivery approach. LCP constructs were synthesised incorporating peptides from the M protein conserved carboxy terminal C-repeat region, the amino terminal type-specific region and from both of these regions. Immunisation with the constructs without adjuvant led to the induction of peptide-specific serum IgG antibody responses, heterologous opsonic antibodies, and complete protection from GAS infection. These data indicate that protective immunity to GAS infection can be evoked using the self-adjuvanting LCP system, and point to the potential application of this system in human mucosal GAS vaccine development.
authors.affiliation: Cooperative Research Centre for Vaccine Technology, The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Division of Infectious Diseases and Immunology, Herston, Brisbane, Qld 4029, Australia. colleenO@qimr.edu.au
authors.forename: Colleen
authors.initials: C
authors.lastname: Olive
authors.forename: K
authors.initials: K
authors.lastname: Hsien
authors.forename: A
authors.initials: A
authors.lastname: Horváth
authors.forename: T
authors.initials: T
authors.lastname: Clair
authors.forename: P
authors.initials: P
authors.lastname: Yarwood
authors.forename: I
authors.initials: I
authors.lastname: Toth
authors.forename: M F
authors.initials: MF
authors.lastname: Good
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D008055:Lipids / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009895:Opsonin Proteins / Q000097:blood; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D013297:Streptococcus pyogenes / Q000235:genetics / Q000276:immunology; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D007074:Immunoglobulin G; D007155:Immunologic Factors; D008055:Lipids; D009895:Opsonin Proteins; D022541:Streptococcal Vaccines; D022223:Vaccines, Subunit; C014689:streptococcal M protein