[Molecular cloning and characterization of the mouse mak-v/Hunk gene promoter].
MAK-V/Hunk is a recently isolated MARK/Par-1-related mammalian protein kinase with yet unknown function. To investigate transcriptional regulation of the mouse mak-v/Hunk gene, we isolated genomic fragment of the mouse mak-v/Hunk promoter region. The mak-v/Hunk promoter has no typical TATA box or CAAT box, is GC-rich and contains CpG-island. Amplification of cDNA ends suggested that transcription initiation site is 156 nt upstream translation initiation site. The 5'-flanking region of the mak-v/Hunk gene was ligated to luciferase reporter gene and possessed functional promoter activity. Luciferase assay with a series of truncated 5'-flanking regions demonstrated the region between nt -508 and -347 has a pronounced stimulating effect on transcription activity. In addition, our data suggest that mak-v/Hunk promoter region might be a target for CpG methylation.
Korobko
E V
EV
Chupikova
N I
NI
Kisilev
S L
SL
Korobko
I V
IV
D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017930:Genes, Reporter; D015698:Genomic Library; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D051381:Rats
D018076:DNA, Complementary; D008156:Luciferases; D011494:Protein Kinases; C417729:Hunk protein, mouse; D017346:Protein Serine-Threonine Kinases