title: [RT-PCR detecting NUP98-HOX fusion gene in leukemia].
abstract: To investigate whether there are NUP98-HOXA, NUP98-HOXB, NUP98-HOXC, NUP98-HOXD fusion genes in leukemia patients in Xinjiang, cellular total RNA was extracted from the bone marrow mononuclear cells, the formaldehyde-agarose gel electrophoresis was used to judge whether RNA was intact, the 17 RT-PCR primers were designed to amplify the predicted fusion junctions and 412 bp GAPDH was used as an internal control, NUP98-HOXA fusion genes were amplified by nested-PCR following reverse transcription. One-step PCR was performed to amplify the other predicted fusion genes. The results showed that RNA was proved to be intact and expression of GAPDH was found in every sample. However, no predicted fusion transcripts were detected in leukemia patients. In conclusion, no NUP98-HOX fusion genes were detected in the samples from Xinjiang.
authors.affiliation: Medical Research Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Bing-Zhao
authors.initials: BZ
authors.lastname: Wen
authors.forename: Ren-Yong
authors.initials: RY
authors.lastname: Lin
authors.forename: Xu
authors.initials: X
authors.lastname: Cao
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Ya Seng
authors.initials: YS
authors.lastname: Ha Li Da
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Lu
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Feng
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: C470262:NUP98-HOXC13 fusion protein, human; D015514:Oncogene Proteins, Fusion