Helicobacter pylori-dependent NF-kappa B activation in newly established Mongolian gerbil gastric cancer cell lines.
Mongolian gerbils are an ideal animal model to explore the role of H. pylori on cancer development. However, there have been no established adenocarcinoma cell lines from this model animal. In the present study, we have established cancer cell lines from a primary gastric cancer tissue of a Mongolian gerbil. The derived cells could be stably attached with H. pylori, revealed under a scanning electron microscope, and easily transplanted to the nude mice. Rapid phosphorylation of IkappaB, Erk1/2, and AKT of these cells was observed by Interleukin-1 beta stimulation, and luciferase reporter gene assay on transcriptional activation of Nuclear Factor kappa B after challenging with either H. pylori NCTC11637 or its isogenic cagE-knockout mutant, H. pylori revealed the cagE-dependent NF-kappaB transcriptional activation. The newly established cancer cell lines from the in vivo gastric carcinogenesis model animal, the Mongolian gerbil, can be used to develop effective therapeutic strategies against gastric cancer, especially in exploring the effect of H. pylori, and thus might greatly contribute to gastric cancer prevention and treatment in humans.
Nozaki
Koji
K
Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya, Aichi 464-8681, Japan.
Tanaka
Harunari
H
Ikehara
Yuzuru
Y
Cao
Xueyuan
X
Nakanishi
Hayao
H
Azuma
Takeshi
T
Yamazaki
Shiho
S
Yamaoka
Yoshio
Y
Shimizu
Nobuyuki
N
Mafune
Ken-ichi
K
Kaminishi
Michio
M
Tatematsu
Masae
M
D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000472:pathogenicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology* / Q000662:veterinary; D014407:Tumor Cells, Cultured
D016328:NF-kappa B