Isolation of palm tocols using supercritical fluid chromatography.
Crude palm oil contains 600 to 1000 ppm of tocols in the form of tocopherols and tocotrienols. These palm tocols have been isolated and analyzed in the past by various chromatographic techniques such as open column chromatography, high-performance liquid chromatography, as well as thin-layer chromatography. Supercritical fluid chromatography (SFC) has emerged as a more advanced chromatographic technique in recent years. The tocols present in palm oil are successfully isolated using SFC. Identification of these tocols is supported by various spectroscopic techniques such as 1H NMR, 13C NMR, and mass spectrometry.
Han
Ng Mei
NM
Malaysian Palm Oil Board, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.
May
Choo Yuen
CY
Ngan
Ma Ah
MA
Hock
Chuah Cheng
CC
Ali Hashim
Mohd
M
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D012015:Reference Standards; D013261:Sterols / Q000302:isolation & purification*; D024505:Tocopherols / Q000302:isolation & purification*
D010938:Plant Oils; D013261:Sterols; D000073878:Palm Oil; D024505:Tocopherols