Separation of etodolac enantiomers by capillary electrophoresis. Validation and application of the chiral method to the analysis of commercial formulations.
Separation of etodolac enantiomers, which exhibit different biological activity and pharmacokinetic profiles, has been achieved using the randomly substituted (2-hydroxy)propyl-beta-cyclodextrin (HP-beta-CD) as chiral selector in capillary electrophoresis. The selection of this CD was made after screening of different CD derivatives of neutral and anionic nature. The effect on the enantioresolution of the buffer concentration and of the degree of substitution (DS) and concentration of the CD as well as of instrumental parameters, such as the capillary temperature and the separation voltage, were studied. The highest resolution of etodolac enantiomers was around 2.5 using 100 mM phosphate buffer (pH 7.0) with 20 mM HP-beta-CD (DS approximately 4.2) and UV detection at 225 (10) nm with a reference wavelength at 360 (50) nm. Validation of the chiral method in terms of selectivity, linearity, precision (instrumental repeatability, method repeatability, intermediate precision), and the limits of detection and quantitation allowed to evaluate its quality to the analysis of etodolac enantiomers in different pharmaceutical preparations containing racemic etodolac.
de Pablos
Raquel Rodríguez
RR
Departamento de Química Analítica, Facultad de Química, Universidad de Alcalá, Ctra. Madrid Barcelona Km. 33.600, E-28871 Alcalá de Henares (Madrid), Spain.
García-Ruiz
Carmen
C
Crego
Antonio L
AL
Marina
M Luisa
ML
D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D017308:Etodolac / Q000032:analysis / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D047392:beta-Cyclodextrins; D017308:Etodolac