title: Use of diagnostic microarrays for determination of virulence gene patterns of Escherichia coli K1, a major cause of neonatal meningitis.
abstract: Forty Escherichia coli strains isolated primarily from neonatal meningitis, urinary tract infections and feces were screened for the presence of virulence genes with a newly developed microarray on the array tube format. A total of 32 gene probes specific for extraintestinal as well as intestinal E. coli pathotypes were included. Eighty-eight percent of the analyzed strains were positive for the K1-specific probe on the microarray and could be confirmed with a specific antiserum against the K1 capsular polysaccharide. The gene for the hemin receptor ChuA was predominantly found in 95% of strains. Other virulence genes associated with K1 and related strains were P, S, and F1C fimbriae specific for extraintestinal E. coli, the genes for aerobactin, the alpha-hemolysin and the cytotoxic necrotizing factor. In two strains, the O157-specific catalase gene and the gene for the low-molecular-weight heat-stable toxin AstA were detected, respectively. A total of 19 different virulence gene patterns were observed. No correlation was observed between specific virulence gene patterns and a clinical outcome. The data indicate that virulence genes typical of extraintestinal E. coli are predominantly present in K1 strains. Nevertheless, some of them can carry virulence genes known to be characteristic of intestinal E. coli. The distribution and combination of virulence genes show that K1 isolates constitute a heterogeneous group of E. coli.
authors.affiliation: Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland.
authors.forename: Bozena
authors.initials: B
authors.lastname: Korczak
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
authors.forename: Gerd
authors.initials: G
authors.lastname: Pluschke
authors.forename: Riccardo
authors.initials: R
authors.lastname: Pfister
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D020869:Gene Expression Profiling; D006801:Humans; D007231:Infant, Newborn; D016920:Meningitis, Bacterial / Q000209:etiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D014774:Virulence