Potential for improving bioavailable zinc in wheat grain (Triticum species) through plant breeding.
A "whole-body" radioassay procedure was used to assess retention and absorption by rats of Zn in mature kernels of whole grain wheat harvested from 28 genotypes (Triticum spp.) grown in nutrient solution supplied with 2 microM ZnSO4 radiolabeled with 65Zn. Grain-Zn concentration differed among genotypes and ranged from 33 to 149 microg g(-1) of dry weight (DW); similarly, grain-Fe concentration varied approximately 4-fold, from 80 to 368 microg g(-1) of DW. Concentrations of Zn and Fe in the grain were positively correlated. Therefore, selecting genotypes high in grain-Zn also tends to increase grain-Fe concentration. Concentrations of myo-inositolhexaphosphate (phytate) in the wheat grain varied from 8.6 to 26.1 micromol g(-1) of DW. Grain intrinsically labeled with 65Zn was incorporated into test meals fed to Zn-depleted rats. All rats readily ate the test meals, so that Zn intake varied directly with grain-Zn concentration. As determined by the percentage of 65Zn absorbed from the test meal, the bioavailability to rats of Zn in the wheat genotypes ranged from about 60 to 82%. The amount of bioavailable Zn (micrograms) in the grain was positively correlated to the amount of Zn accumulated in the grain. There was a significant negative correlation between grain-phytate levels and percentage of Zn absorbed from the wheat grain, but the effect was not large. These results demonstrate that concentrations of Zn in whole-wheat grain, as well as amounts of bioavailable Zn in the grain, can be increased significantly by using traditional plant-breeding programs to select genotypes with high grain-Zn levels. Increasing the amount of Zn in wheat grain through plant-breeding contrivances may contribute significantly to improving the Zn status of individuals dependent on whole grain wheat as a staple food.
Welch
Ross M
RM
U.S. Plant, Soil and Nutrition Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Cornell University, Ithaca, New York 14853, USA. rmw1@cornell.edu
House
William A
WA
Ortiz-Monasterio
Ivan
I
Cheng
Z
Z
D000818:Animals; D001682:Biological Availability; D001947:Breeding; D005838:Genotype; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D014908:Triticum / Q000737:chemistry* / Q000235:genetics*; D015032:Zinc / Q000032:analysis / Q000493:pharmacokinetics*
D015032:Zinc