HPLC-DAD determination of plasma levels of the antipsychotic risperidone and its main metabolite for toxicological purposes.
A new, rapid analytical method, based on liquid chromatography with diode array detection, has been developed and applied to the determination of risperidone and its main active metabolite 9-hydroxyrisperidone in human plasma. The chromatographic separation was obtained on a C8 (150 x 4.6 mm, 5 microm) column, using a mobile phase composed of acetonitrile (27%) and a pH 3.0 phosphate buffer (73%). A sample clean-up procedure was carried out by using C8 cartridges and eluting the analytes with methanol. The extraction yield was highly satisfactory for both analytes, with average absolute recovery values of about 95%. The experimental conditions permitted the quantitative determination of risperidone and 9-hydroxyrisperidone with high precision (RSD < 3.6%) and satisfactory sensitivity (LOQ = 4 ng mL(-1)). The method was applied to plasma samples from a patient who had tried to poison himself with 150 mg of risperidone, and was undergoing polypharmacy.
Raggi
Maria Augusta
MA
Dipartimento di Scienze Farmaceutiche, Facoltà di Farmacia, Alma Mater Studiorum--Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy. mariaaugusta.raggi@unibo.it
Bugamelli
Francesca
F
Sabbioni
Cesare
C
Saracino
Maria Addolorata
MA
Petio
Carmine
C
D014150:Antipsychotic Agents / Q000009:adverse effects / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D018967:Risperidone / Q000009:adverse effects / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*; D014116:Toxicology / Q000379:methods*
D014150:Antipsychotic Agents; D018967:Risperidone