The C terminus of MINT forms homodimers and abrogates MINT-mediated transcriptional repression.
Notch signaling plays a pivotal role in numerous cell fate determination events during development, and therefore its regulation has been studied intensively. MSX2-interacting nuclear target protein (MINT) modifies the Notch signaling by interacting with and inhibiting the downstream transcription factor RBP-J/CBF-1 of Notch. In this study, by a yeast two hybrid screening, we found that the C terminal fragment of MINT interacted with each other. We confirmed the interaction between two MINT C terminal fragments both in vitro and in vivo. We further demonstrated that the overexpression of the C terminal fragment of MINT cancelled its inhibitory effect on the transactivation of an RBP-J-dependent promoter by Notch. These results suggest that MINT may form a dimer or multimer in cells through its C terminus, and that the C terminal fragment of MINT may work as its dominant-negative version.
Li
Junlin
J
Department of Medical Genetics and Developmental Biology, State Key Laboratory of GI Cancer Biology, Fourth Military Medical University, Xian 710032, China.
Li
Junfeng
J
Yang
Xi
X
Qin
Hongyan
H
Zhou
Peng
P
Liang
Yingmin
Y
Han
Hua
H
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D008565:Membrane Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D016601:RNA-Binding Proteins; D051880:Receptors, Notch; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
C079091:APBA1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; C084119:MSX2 protein; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C497570:RBPJ protein, human; D016601:RNA-Binding Proteins; D051880:Receptors, Notch; C121253:Spen protein, mouse