General strategies for efficient adjuvant incorporation of recombinant subunit immunogens.
We have previously reported strategies for Escherichia coli production of recombinant immunogens fused to hydrophobic peptides or lipid tags to improve their capacity to be incorporated into an adjuvant formulation, e.g., immunostimulating complexes (iscoms). Recently, we also explored the strong interaction between biotin and streptavidin to achieve iscom association of recombinant immunogens. Plasmodium falciparum,Toxoplasma gondii and Neospora caninum antigens have served as model immunogens in the different studies. Generated fusion proteins have been found to be successfully incorporated into iscoms and high-titer antigen-specific antibody responses have been obtained upon immunization of mice. We believe that the different concepts presented, utilizing either hydrophobic peptide or lipid tags, or the recently explored biotin-streptavidin principle, offer convenient methods to achieve efficient adjuvant incorporation of recombinant immunogens.
Wikman
Maria
M
Department of Biotechnology, AlbaNova University Center, Kungl Tekniska Högskolan (KTH), SE-10691 Stockholm, Sweden.
Friedman
Mikaela
M
Pinitkiatisakul
Sunan
S
Andersson
Christin
C
Hemphill
Andrew
A
Lövgren-Bengtsson
Karin
K
Lundén
Anna
A
Ståhl
Stefan
S
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D001710:Biotin; D004926:Escherichia coli / Q000235:genetics; D016882:ISCOMs; D007114:Immunization; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D019809:Streptavidin; D022223:Vaccines, Subunit / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics
D000941:Antigens; D016882:ISCOMs; D007155:Immunologic Factors; D008055:Lipids; D011993:Recombinant Fusion Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D001710:Biotin; D019809:Streptavidin