[Genetic polymorphism of human immunodeficiency virus coreceptor CCR5Delta32 and CCR2-64I alleles in Chinese Yi Ethnic group in Sichuan].
OBJECTIVE
To explore genetic polymorphisms CCR5 of HIV coreceptor and CCR2 in Chinese Yi Ethnic group in Sichuan.


METHODS
Genomic DNA samples were obtained from 119 healthy individuals and 88 HIV-1 infected individuals of Chinese Yi Ethnic group in Sichuan. Polymerase chain reaction (PCR), cloning and gene sequencing techniques were employed to identify the genotype of CCR5Delta32; PCR-restriction fragment length polymorphism (RFLP) and gene sequencing were employed to identify the CCR2-64I alleles.


RESULTS
At CCR5 locus, 2 heterozygotes (CCR5-wt/Delta32) and none homozygote (CCR5-Delta32/Delta32) were observed in 119 healthy individuals, allelic frequency of CCR5-Delta32 was 0.84%; No mutant was found in 88 HIV-1 infected individuals. At CCR2 locus, 26 heterozygotes (CCR2-64V/64I) and two homozygotes (CCR2-64I/64I) were observed in healthy individuals but the allelic frequency CCR2-64I was 12.61%. Among infected individuals, 12 heterozygotes (CCR2-64V/64I) and 7 homozygotes (CCR2-64I/64I) were observed and the allelic frequency CCR2-64I was 13.27%. Statistical analysis revealed that the differences of both loci between healthy and infected individuals were insignificant. Both loci were consistent with the Hardy-Weinberg equilibrium in the two different groups.


CONCLUSIONS
The polymorphism of CCR5Delta32 and CCR2-64I alleles from Chinese Yi Ethnic group was detected which was of significance for the evaluation of genetic resistance to HIV-1 infection in Chinese population.
Deng
Xiao-Ling
XL
National Center for Sexually Transmitted Disease and Acquired Immune Deficiency Syndrome Control and Prevention, Chinese Center for Disease Centrol and Prevention, Beijing 100050, China.
Hong
Kun-Xue
KX
Chen
Jian-Ping
JP
Ruan
Yu-Hua
YH
Xu
Ming-Yan
MY
Qin
Guang-Ming
GM
Li
Ke
K
Xing
Hui
H
Shao
Yi-Ming
YM
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D002681:China / Q000208:ethnology; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D019713:Receptors, CCR5 / Q000235:genetics*; D015728:Receptors, HIV / Q000235:genetics*
D019713:Receptors, CCR5; D015728:Receptors, HIV