Beta-chemokine receptor expression in idiopathic inflammatory myopathies.
Beta-chemokines attract and activate T cells and monocytes and have a key role in chronic inflammation. Certain beta-chemokines, such as monocyte chemoattractant protein-1 (MCP-1), have been reported to be upregulated in the idiopathic inflammatory myopathies (IIM). We studied the distribution of beta-chemokine receptors in polymyositis (PM), sporadic inclusion-body myositis (sIBM), dermatomyositis (DM), and control samples. CCR1-5 were localized to blood vessels in all samples. In addition, increased endothelial expression of CCR2A was observed in IIM. Subsets of inflammatory cells, identified as macrophages and T cells, in all three types of IIM expressed CCR2A, CCR2B, CCR3, CCR4, and CCR5. In contrast to an earlier report, we found CCR2B to be the most prominent MCP-1 receptor on inflammatory cells in IIM, especially in PM and sIBM. Strong CCR4 expression was present on myonuclei of regenerating muscle fibers. The prominence of the CCR2 receptors further underlines the importance of the interaction with their ligand MCP-1 in the immunopathogenesis of IIM and puts CCR2B forward as a potential target for future therapeutic intervention.
De Paepe
Boel
B
Department of Neurology, Neuromuscular Laboratory, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
De Bleecker
Jan L
JL
D001808:Blood Vessels / Q000276:immunology* / Q000473:pathology; D015153:Blotting, Western; D018932:Chemokine CCL2 / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D042783:Endothelial Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology; D018482:Muscle, Skeletal / Q000098:blood supply / Q000276:immunology* / Q000473:pathology*; D009220:Myositis / Q000276:immunology* / Q000473:pathology*; D054390:Receptors, CCR2; D054398:Receptors, CCR4; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
C111835:CCL2 protein, human; C515334:CCR2 protein, human; C515354:CCR4 protein, human; D018932:Chemokine CCL2; D054390:Receptors, CCR2; D054398:Receptors, CCR4; D019707:Receptors, Chemokine