title: Prism-based excitation wavelength selection for multicolor fluorescence coincidence measurements.
abstract: We have designed and constructed a prism setup for multiple-color confocal fluorescence experiments. The prism setup permits easy selection of any color or any combination of colors from a multicolor light source, such as a mixed-gas argon-krypton-ion laser. The selected colors emerging from the prism setup are, by design, optimally overlapped in the focus of a high-numerical-aperture objective, such as that commonly used in single-molecule fluorescence experiments. The various excitation powers can be easily adjusted in this setup. We will exemplify the potential of this setup in single-molecule fluorescence emission spectroscopy.
authors.affiliation: Institute for Biomedical Technology, Department of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands. r.kassies@utwente.nl
authors.forename: Roel
authors.initials: R
authors.lastname: Kassies
authors.forename: Aufried
authors.initials: A
authors.lastname: Lenferink
authors.forename: Ine
authors.initials: I
authors.lastname: Segers-Nolten
authors.forename: Cees
authors.initials: C
authors.lastname: Otto