[Determination of iron(III) in Chinese herbal medicine and tea based on fluorescence quenching of 2,4-dichro-phenylfluorone].
A new spectrofluorimetric method for the determination of trace amount of Fe(III) has been developed. This method is based on the fluorescence quenching of 2,4-dichro-phenylfluorone due to the formation of complex Fe(III)-DCIPF. In pH 5.2-5.9 buffer solution, Fe (III) and DCIPF react on each other to form a red complex. Its composition was established by method of continuous variations and molar-ratio as Fe(III): DCIPF = 1:4, the excitation and emission wavelengths were found to be 282 and 560 nm, respectively. There is a linear relationship in the range 4-24 ng x mL(-1) for Fe(III). This method is simple and rapid, and has been applied to the determination of trace iron in Chinese herbal medicine and tea samples with satisfactory results.
Dai
Gang
G
Institute of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China.
Aodeng
Gao-wa
GW
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005290:Ferric Compounds / Q000032:analysis / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D005453:Fluorescence; D007501:Iron / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013059:Spectrum Analysis, Raman; D013662:Tea
D004365:Drugs, Chinese Herbal; D005290:Ferric Compounds; D005452:Fluoresceins; D013662:Tea; C010159:fluorone Black; D007501:Iron