title: Biosensor analysis of the interaction between drug compounds and liposomes of different properties; a two-dimensional characterization tool for estimation of membrane absorption.
abstract: The interactions between 78 drug compounds and immobilised liposomes were investigated using an assay based on surface plasmon resonance technology. The drugs were screened at a single concentration and allowed to interact simultaneously with two different types of liposomes. When the drug-liposome responses are plotted against one another they generally fall into three distinct bands: low response-low percent fraction absorbed in humans (Fa), medium response-medium Fa, and high response-high Fa. For drugs with medium to high Fa values, basic compounds could be resolved from acidic and neutral compounds to a large extent. This technique has the potential to be utilized as a screening tool for binning novel compounds into low, medium, or high Fa based on a simple experimental measurement. The assay was applied to 11 kinase inhibitors, 9 thrombin inhibitors, and 11 carbonic anhydrase inhibitors highlighting a subset that may have incomplete intestinal absorption (low to medium Fa). Assay conditions were optimized making the assay suitable for routine analysis and for compound characterization early in drug discovery where solubility may be an issue.
authors.affiliation: Biacore AB, Rapsgatan 7, S-754 50 Uppsala, Sweden. asa.frostellkarlsson@biacore.com <asa.frostellkarlsson@biacore.com>
authors.forename: Asa
authors.initials: A
authors.lastname: Frostell-Karlsson
authors.forename: Helena
authors.initials: H
authors.lastname: Widegren
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Green
authors.forename: Markku D
authors.initials: MD
authors.lastname: Hämäläinen
authors.forename: Lena
authors.initials: L
authors.lastname: Westerlund
authors.forename: Robert
authors.initials: R
authors.lastname: Karlsson
authors.forename: Katherine
authors.initials: K
authors.lastname: Fenner
authors.forename: Han
authors.initials: H
authors.lastname: van de Waterbeemd
mesh_terms: D000042:Absorption; D015374:Biosensing Techniques; D002021:Buffers; D004121:Dimethyl Sulfoxide; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008567:Membranes, Artificial; D008954:Models, Biological; D008970:Molecular Weight; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D002021:Buffers; D008081:Liposomes; D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations; D004121:Dimethyl Sulfoxide