Involvement of cyclic GMP-dependent protein kinase in nitrous oxide-induced anxiolytic-like behavior in the mouse light/dark exploration test.
The second messenger cyclic GMP (cGMP) plays a role in the anxiolytic-like behavioral response of mice to nitrous oxide (N2O). This study was conducted to determine whether this behavioral effect of N2O is affected by inhibition of cGMP-dependent protein kinase (PKG). N2O-induced behavior in the light/dark exploration test was significantly attenuated by the PKG inhibitors H-8 and Rp-8-pCPT-cGMPS but not Rp-8-pCPT-cAMPS, an inhibitor of cAMP-dependent protein kinase. These findings implicate PKG in the mediation or modulation of the anxiolytic-like behavioral response to N2O.
Li
Shuang
S
Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA 99164-6534, USA.
Doss
Jason C
JC
Hardee
Elizabeth J
EJ
Quock
Raymond M
RM
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001007:Anxiety / Q000139:chemically induced / Q000201:enzymology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000502:physiology; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D017869:Cyclic GMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D003624:Darkness; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology*; D007546:Isoquinolines / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009609:Nitrous Oxide; D013873:Thionucleotides / Q000494:pharmacology
C090860:8-(4-chlorophenylthio)guanosine 3',5'-cyclic monophosphorothioate; C105593:8-bromoadenosine-3',5'-cyclic monophosphorothioate; D004791:Enzyme Inhibitors; D007546:Isoquinolines; D013873:Thionucleotides; D015124:8-Bromo Cyclic Adenosine Monophosphate; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D017868:Cyclic AMP-Dependent Protein Kinases; D017869:Cyclic GMP-Dependent Protein Kinases; D006152:Cyclic GMP; D009609:Nitrous Oxide