title: Polymorphism in Nucleotide Sequence of Mitochondrial Intergenic Region in Scleractinian Coral (Galaxea fascicularis).
abstract: A region of 826 bp that is unlikely to code for a protein, ribosomal RNA, or transfer RNA was identified between the cytochrome b and NADH-ubiquinone oxidoreductase chain 2 loci in the mitochondrial DNA of the scleractinian reef coral Galaxea fascicularis. Nucleotide sequences were determined in a part (625 bp) of this intergenic region in 95 individuals collected at 9 sites in the Ryukyu Archipelago in southwestern Japan. A total of 8 haplotypes were found, and a deletion of 290 bp was found in 3 of them. Significant differences were found in frequencies of the haplotypes at 3 sampling sites. The presence or absence of the deletion was highly correlated with the hard or soft morphotype. The deletion was found in the majority of hard-type colonies, but in a small fraction of soft-type individuals.
authors.affiliation: Division of Marine Biosciences, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan. toshi@ori.u-tokyo.ac.jp
authors.forename: Toshiki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Nishida
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Defny S
authors.initials: DS
authors.lastname: Wewengkang
authors.forename: Michio
authors.initials: M
authors.lastname: Hidaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D037421:Anthozoa / Q000033:anatomy & histology / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D021901:DNA, Intergenic / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D007564:Japan; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial