Identification and characterization of murine DNAM-1 (CD226) and its poliovirus receptor family ligands.
The leukocyte adhesion molecule DNAM-1 (CD226) is a member of the immunoglobulin superfamily and constitutively expressed on the majority of CD4+ and CD8+ T lymphocytes, natural killer (NK) cells, monocytes/macrophages, and a subset of B lymphocytes. The poliovirus receptor (PVR; CD155) and its family member nectin 2 (CD112) have recently been identified as the ligands for DNAM-1. Interaction of DNAM-1 with the ligands induces NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. However, in vivo function of the receptor-ligand interaction has remained unclear. Here, we identified murine DNAM-1 and PVR homologues that physically and functionally bind each other. We demonstrated that ligand binding of murine DNAM-1 induced a costimulatory signal in antigen-specific CD8+ T cells. These results should provide a useful animal model to explore a role of DNAM-1 in immune responses in vivo.
Tahara-Hanaoka
Satoko
S
Department of Immunology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, Center for TARA, University of Tsukuba, Ibaraki 305-8575, Japan.
Miyamoto
Akitomo
A
Hara
Ayumi
A
Honda
Shin-ichiro
S
Shibuya
Kazuko
K
Shibuya
Akira
A
D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology*; D001665:Binding Sites; D002478:Cells, Cultured; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011991:Receptors, Virus / Q000737:chemistry* / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology*; D000098862:T Lineage-Specific Activation Antigen 1
D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D008024:Ligands; D008565:Membrane Proteins; D011991:Receptors, Virus; C066100:poliovirus receptor