Evaluation of a soybean lectin-based method for the enrichment of erythroblasts.
We performed a comparative study of the enrichment of erythroblasts by a soybean agglutinin galactose-specific lectin method and a standardized magnetic cell-sorting (MACS) protocol. Blood samples, obtained from 11 pregnant women at between 11 and 40 weeks of gestation, were split and examined by each method in parallel. The number of erythroblasts recovered by the lectin method was approximately eightfold higher than the number obtained by MACS. Our data suggest that the lectin-based method may provide a better approach for the enrichment of rare fetal erythroblasts from maternal blood.
Babochkina
Tatiana
T
Laboratory for Prenatal Medicine, University Women's Hospital/Department of Research, Spitalstr. 21, CH 4031 Basel, Switzerland.
Mergenthaler
Susanne
S
Lapaire
Olaf
O
Kiefer
Vivian
V
Yura
Hirofumi
H
Koike
Kimie
K
Holzgreve
Wolfgang
W
Hahn
Sinuhe
S
D002469:Cell Separation / Q000379:methods*; D004900:Erythroblasts / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D018189:Immunomagnetic Separation; D007202:Indicators and Reagents; D037121:Plant Lectins; D011247:Pregnancy; D030262:Soybean Proteins; D013025:Glycine max; D010835:Phytohemagglutinins
D007202:Indicators and Reagents; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins