title: Study of stem cell function using microarray experiments.
abstract: DNA Microarrays are used to simultaneously measure the levels of thousands of mRNAs in a sample. We illustrate here that a collection of such measurements in different cell types and states is a sound source of functional predictions, provided the microarray experiments are analogous and the cell samples are appropriately diverse. We have used this approach to study stem cells, whose identity and mechanisms of control are not well understood, generating Affymetrix microarray data from more than 200 samples, including stem cells and their derivatives, from human and mouse. The data can be accessed online (StemBase; http://www.scgp.ca:8080/StemBase/).
authors.affiliation: Ontario Genomics Innovation Centre, Ottawa Health Research Institute, Molecular Medicine Program, 501 Smyth Road, Ottawa, Canada K1H 8L6.
authors.forename: Carolina
authors.initials: C
authors.lastname: Perez-Iratxeta
authors.forename: Gareth
authors.initials: G
authors.lastname: Palidwor
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Porter
authors.forename: Neal A
authors.initials: NA
authors.lastname: Sanche
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Huska
authors.forename: Brian P
authors.initials: BP
authors.lastname: Suomela
authors.forename: Enrique M
authors.initials: EM
authors.lastname: Muro
authors.forename: Paul M
authors.initials: PM
authors.lastname: Krzyzanowski
authors.forename: Evan
authors.initials: E
authors.lastname: Hughes
authors.forename: Pearl A
authors.initials: PA
authors.lastname: Campbell
authors.forename: Michael A
authors.initials: MA
authors.lastname: Rudnicki
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Andrade
mesh_terms: D000818:Animals; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells / Q000502:physiology*