title: The mouse CD1d cytoplasmic tail mediates CD1d trafficking and antigen presentation by adaptor protein 3-dependent and -independent mechanisms.
abstract: The short cytoplasmic tail of mouse CD1d (mCD1d) is required for its endosomal localization, for the presentation of some glycolipid Ags, and for the development of Valpha14i NKT cells. This tail has a four-amino acid Tyr-containing motif, Tyr-Gln-Asp-Ile (YQDI), similar to those sequences known to be important for the interaction with adaptor protein complexes (AP) that mediate the endosomal localization of many different proteins. In fact, mCD1d has been shown previously to interact with the AP-3 adaptor complex. In the present study, we mutated each amino acid in the YQDI motif to determine the importance of the entire motif sequence in influencing mCD1d trafficking, its interaction with adaptors, and its intracellular localization. The results indicate that the Y, D, and I amino acids are significant functionally because mutations at each of these positions altered the intracellular distribution of mCD1d and reduced its ability to present glycosphingolipids to NKT cells. However, the three amino acids are not all acting in the same way because they differ with regard to how they influence the intracellular distribution of CD1d, its rate of internalization, and its ability to interact with the mu subunit of AP-3. Our results emphasize that multiple steps, including interactions with the adaptors AP-2 and AP-3, are required for normal trafficking of mCD1d and that these different steps are mediated by only a few cytoplasmic amino acids.
authors.affiliation: Division of Developmental Immunology, Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA.
authors.forename: Anna P
authors.initials: AP
authors.lastname: Lawton
authors.forename: Theodore I
authors.initials: TI
authors.lastname: Prigozy
authors.forename: Laurent
authors.initials: L
authors.lastname: Brossay
authors.forename: Bo
authors.initials: B
authors.lastname: Pei
authors.forename: Archana
authors.initials: A
authors.lastname: Khurana
authors.forename: Donald
authors.initials: D
authors.lastname: Martin
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Zhu
authors.forename: Kira
authors.initials: K
authors.lastname: Späte
authors.forename: Megda
authors.initials: M
authors.lastname: Ozga
authors.forename: Stefan
authors.initials: S
authors.lastname: Höning
authors.forename: Oddmund
authors.initials: O
authors.lastname: Bakke
authors.forename: Mitchell
authors.initials: M
authors.lastname: Kronenberg
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000378:metabolism; D033963:Adaptor Protein Complex 3 / Q000378:metabolism*; D033983:Adaptor Protein Complex mu Subunits / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D018949:Antigens, CD1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D055610:Antigens, CD1d; D002460:Cell Line; D006028:Glycosphingolipids / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
chemical_list: D033962:Adaptor Protein Complex 2; D033963:Adaptor Protein Complex 3; D033983:Adaptor Protein Complex mu Subunits; D018949:Antigens, CD1; D055610:Antigens, CD1d; D006028:Glycosphingolipids; D011994:Recombinant Proteins