(13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion.
(13)C-(13)C NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, (15)N, (13C) and 70% (2)H labeled). (13)C-(13)C couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time tau(m) of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C' and C(beta). The interpretation was confirmed by simulations. This approach broadens the range of applicability of (13)C-(13)C NOESY spectroscopy.
Bertini
Ivano
I
Magnetic Resonance Center and Department of Chemistry, University of Florence, Italy. bertini@cerm.unifi.it
Felli
Isabella C
IC
Kümmerle
Rainer
R
Luchinat
Claudio
C
Pierattelli
Roberta
R
D000465:Algorithms; D002247:Carbon Isotopes / Q000737:chemistry; D004058:Diffusion; D006801:Humans; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D013113:Spin Labels; D013482:Superoxide Dismutase