Phylogenetic and biochemical characterisation of a recombinant laccase from Trametes versicolor.
Laccases are important enzymes for bioremediation and the best-characterised are from the fungus Trametes versicolor. Here, we describe the cloning and characterisation of a new variant of laccase from T. versicolor and its expression in Saccharomyces cerevisiae. We have performed a sequence-based analysis of Trametes laccases that leads to a proposal for a new nomenclature of this fungus laccases according to their phylogenetic relationships since their nomenclature based on IPs is ambiguous. We also describe the kinetic properties of the recombinant enzyme.
Necochea
Rosalía
R
Departamento de Biotecnología Ambiental, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Mor. 62210, México.
Valderrama
Brenda
B
Díaz-Sandoval
Silvia
S
Folch-Mallol
Jorge Luis
JL
Vázquez-Duhalt
Rafael
R
Iturriaga
Gabriel
G
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D001673:Biodegradation, Environmental; D042845:Laccase / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D011994:Recombinant Proteins; D042845:Laccase