The influence of gellan gum on the transfer of fluorescein dextran across rat nasal epithelium in vivo.
The nasal uptake of a 3000 Da fluorescein dextran (FD3) was investigated in rats, using fluorescence microscopy. The uptake from a formulation containing deacetylated gellan gum, an in situ gelling agent, was compared to that from a mannitol solution. Additionally, the rheological behavior of the gellan gum in water and saline was studied. It was shown that the gellan gum solution was easily administered owing to its low viscosity, and upon contact with the mucosa, a gel was formed. The epithelial uptake and transfer of FD3 appeared to be increased and prolonged using the gellan gum formulation. This increase was not accompanied by qualitative changes of the epithelial FD3 distribution or any visible harmful effects.
Jansson
Björn
B
Department of Pharmacy, Uppsala University, Uppsala, Sweden.
Hägerström
Helene
H
Fransén
Nelly
N
Edsman
Katarina
K
Björk
Erik
E
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D003911:Dextrans / Q000737:chemistry / Q000493:pharmacokinetics*; D005452:Fluoresceins / Q000737:chemistry / Q000493:pharmacokinetics*; D007552:Isotonic Solutions; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology / Q000379:methods; D014867:Water / Q000378:metabolism
D003911:Dextrans; D005452:Fluoresceins; D007552:Isotonic Solutions; D011135:Polysaccharides, Bacterial; C052526:fluorescein-dextran; D014867:Water; C048288:gellan gum