Transcriptional analysis of the principal cell division gene, ftsZ, of Mycobacterium tuberculosis.
Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies identified six ftsZ transcripts and their promoters in the ftsQ open reading frame and ftsQ-ftsZ intergenic region. The presence of multiple promoters reflects the requirement to maintain a high basal level of, or to differentially regulate, FtsZ expression during different growth conditions of the pathogen in vivo.
Roy
Sougata
S
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore-560012, India.
Ajitkumar
Parthasarathi
P
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002874:Chromosome Mapping; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000502:physiology*; D021901:DNA, Intergenic / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000502:physiology*; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000502:physiology*; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D021901:DNA, Intergenic; C071525:FtsZ protein, Bacteria