title: Diagnostic system for rapid and sensitive differential detection of pathogens.
abstract: Naturally emerging and deliberately released pathogens demand new detection strategies to allow early recognition and containment. We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences and report its application for detecting 22 respiratory pathogens in clinical samples.
authors.affiliation: Columbia University, New York, NY 10032, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.forename: Mark
authors.initials: M
authors.lastname: Kokoris
authors.forename: Omar
authors.initials: O
authors.lastname: Jabado
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Neil
authors.initials: N
authors.lastname: Renwick
authors.forename: Vishal
authors.initials: V
authors.lastname: Kapoor
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Casas
authors.forename: Francisco
authors.initials: F
authors.lastname: Pozo
authors.forename: Ron
authors.initials: R
authors.lastname: Limberger
authors.forename: Pilar
authors.initials: P
authors.lastname: Perez-Brena
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D001424:Bacterial Infections / Q000175:diagnosis*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology / Q000821:virology; D006801:Humans; D017817:Nasal Lavage Fluid / Q000382:microbiology / Q000821:virology; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013183:Sputum / Q000382:microbiology / Q000821:virology; D014777:Virus Diseases / Q000175:diagnosis*
chemical_list: D012329:RNA, Bacterial; D012367:RNA, Viral