Glucagon-like peptide 2 is an endogenous mediator of postresection intestinal adaptation.
BACKGROUND
After massive small bowel resection, the remnant intestine undergoes compensatory adaptation. We tested the hypothesis that glucagon-like peptide-2 (GLP-2) is an endogenous mediator of postresection intestinal adaptation.


METHODS
Rats were allocated to 1 of 4 groups: groups 1 and 2 rats underwent mid-small bowel transection and reanastomosis; groups 3 and 4 rats underwent 75% mid-small bowel resection and reanastomosis. Groups 2 and 4 rats were administered 1.8 mg of antirat GLP-2 antibody twice daily beginning immediately after the surgical procedure; groups 1 and 3 rats were administered rabbit serum (control). Ileal specimens were harvested on postoperative day 7.


RESULTS
Ileal mucosa from group 3 animals displayed morphologic and proliferative indices of adaptation. Each of these indices of adaptation was inhibited by GLP-2 immunoneutralization (group 4). Morphologic and proliferative parameters in the ileum from animals that had undergone transection with reanastomosis were unaffected by GLP-2 immunoneutralization.


CONCLUSIONS
These results suggest that GLP-2 is an endogenous mediator of postresection intestinal adaptation.
Perez
Alexander
A
Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA.
Duxbury
Mark
M
Rocha
Flavio G
FG
Ramsanahie
Anthony P
AP
Farivar
Robert S
RS
Varnholt
Heike
H
Ito
Hiromichi
H
Wong
Helen
H
Rounds
Jan
J
Zinner
Michael J
MJ
Whang
Edward E
EE
Ashley
Stanley W
SW
D000222:Adaptation, Physiological; D000714:Anastomosis, Surgical; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D004195:Disease Models, Animal; D053767:Glucagon-Like Peptide 2; D004763:Glucagon-Like Peptides; D007082:Ileum / Q000254:growth & development / Q000601:surgery*; D007413:Intestinal Mucosa / Q000254:growth & development* / Q000378:metabolism; D007421:Intestine, Small / Q000254:growth & development / Q000601:surgery; D008297:Male; D010455:Peptides / Q000276:immunology / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D053767:Glucagon-Like Peptide 2; D010455:Peptides; D004763:Glucagon-Like Peptides