title: Highly sensitive near-infrared fluorescent probes for nitric oxide and their application to isolated organs.
abstract: Novel near-infrared (NIR) fluorescent probes for nitric oxide (NO) have been designed, synthesized, and evaluated. Their NIR fluorescence was increased in an NO concentration-dependent manner under physiological conditions, and their reaction efficiency with NO was at least 53 times higher than that of a widely used NO probe, DAF-2. They were confirmed to function in isolated intact rat kidneys. Because NIR light can penetrate deeply into tissues, these probes may have potential for in vivo NO imaging.
authors.affiliation: Graduate School of Pharmaceutical Sciences, and Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Eita
authors.initials: E
authors.lastname: Sasaki
authors.forename: Hirotatsu
authors.initials: H
authors.lastname: Kojima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nishimatsu
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Hirata
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D010655:Phenylenediamines / Q000737:chemistry*; D051381:Rats; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000379:methods
chemical_list: C111205:4,5-diaminofluorescein; D002232:Carbocyanines; D005456:Fluorescent Dyes; D010655:Phenylenediamines; D009569:Nitric Oxide; C034193:1,2-diaminobenzene; D019793:Fluorescein