Role of CC chemokine CCL6/C10 as a monocyte chemoattractant in a murine acute peritonitis.
The aim of this study was to determine the role of CC chemokine CCL6/C10 in acute inflammation. Intraperitoneal injection of thioglycollate increased peritoneal CCL6, which peaked at 4 h and remained elevated at 48 h. Neutralization of CCL6 significantly inhibited the macrophage infiltration (34-48% reduction), but not other cell types, without decreasing the other CC chemokines known to attract monocytes/macrophages. CCL6 was expressed in peripheral eosinophils and elicited macrophages, but not in elicited neutrophils. Peritoneal CCL6 level was not decreased in granulocyte-depleted mice where eosinophil influx was significantly impaired. Thus, CCL6 appears to contribute to the macrophage infiltration that is independent of other CC chemokines. Eosinophils pre-store CCL6, but do not release CCL6 in the peritoneum in this model of inflammation.
LaFleur
Andrew M
AM
Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Lukacs
Nicholas W
NW
Kunkel
Steven L
SL
Matsukawa
Akihiro
A
D000208:Acute Disease; D000818:Animals; D001483:Base Sequence; D019742:Chemokines, CC; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D004195:Disease Models, Animal; D004804:Eosinophils / Q000502:physiology; D005260:Female; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000502:physiology; D051379:Mice; D009000:Monocytes / Q000502:physiology*; D009500:Neutralization Tests; D010538:Peritonitis / Q000209:etiology / Q000503:physiopathology*; D013864:Thioglycolates / Q000633:toxicity
D019742:Chemokines, CC; D016207:Cytokines; D018076:DNA, Complementary; D018836:Inflammation Mediators; D013864:Thioglycolates; C070959:Ccl6 protein, mouse