Enzymatic clipping of DNA wires coated with magnetic nanoparticles.
The study describes how DNA coated with magnetic nanoparticles remains biologically active and accessible to the BamH1 restriction enzyme. Long DNA molecules are coated with magnetic nanoparticles using electrostatic interactions. The coated, stretched, and surface-bound DNA is incubated in the restriction enzyme that specifically recognizes any strand containing the GGATCC base sequence and clips the DNA. We show that, despite the presence of the nanoparticles on the DNA, the enzyme is still able to recognize the cleavage site and effectively digest the assembly.
Kinsella
Joseph M
JM
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Ivanisevic
Albena
A
D010582:Bacteriophage lambda / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry*; D015245:Deoxyribonuclease BamHI / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D008280:Magnetics; D049329:Nanostructures / Q000737:chemistry*; D055672:Static Electricity
D004279:DNA, Viral; D005290:Ferric Compounds; C000499:ferric oxide; D015245:Deoxyribonuclease BamHI