Reproductive status in female perch (Perca fluviatilis) outside a sewage treatment plant processing leachate from a refuse dump.
Reproductive failure and endocrine disruption in female perch (Perca fluviatilis) and female brook trout (Salvelinus fontinalis), naturally exposed to toxic leachate from a municipal refuse dump, has raised concerns about the reproductive status in female fish outside the sewage treatment plant (STP), which now treats the same leachate. Female perch from the receiving water, Lake Siljan, were therefore sampled in a gradient from the STP in Fornby and Osterviken and compared with reference females from Isunda or Solleron. A high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS) technique enabled the analysis of circulating steroids in blood plasma: the sex steroids progesterone (P), 17alpha-hydroxyprogesterone (17alpha-OHP), androstenedione (A), testosterone (T), estrone (E1), and 17beta-oestradiol (E2), the maturation-inducing hormone (MIH) 17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha,20beta-P), and the glucocorticoids 11-deoxycortisol (11-DC) and cortisol (C). Body and organ measurements, ratio of sexually mature (SM) females, and frequencies of body lesions, were also recorded. The percentages of SM females found in Fornby on Oct 23 (83.8%) and in Osterviken on May 14 (54.0%) were lower compared with Isunda (100%). The lack of differences in gonadosomatic index (GSI) and liver-somatic index (LSI) indicated a normal vitellogenesis in SM females from Fornby and Osterviken. No differences in circulating levels of steroids were observed between the sites, except at the time of spawning, when elevated P and 17alpha-OHP levels in females from Osterviken indicated a steroidogenic shift from the synthesis of sex steroids towards the production of the MIH 17alpha,20beta-P. The significant peak-levels of both 17alpha,20beta-P and 11-DC in female perch from Osterviken at the same time suggest that both are involved in controlling final oocyte maturation in perch. The elevated levels of androgens (A and T) in females caught on the same occasion in Fornby implied that these females were at an earlier stage of oocyte maturation, prior to the steroidogenic shift towards MIH-production. This study confirms the results of an earlier study revealing low numbers of SM female perch outside the STP in Fornby. The lack of associated effects on organ indexes and circulating levels of steroids does not indicate exposure to endocrine disrupting substances (EDSs) from the treated sewage. The consistently high numbers of SM females in the northern part of Siljan suggest, however, that natural factors are not responsible for the reproductive failures in the southern part of the lake.
Noaksson
Erik
E
Laboratory for Aquatic Ecotoxicology, Institute of Applied Environmental Research (ITM), Stockholm University, SE-106 91 Stockholm, Sweden. erik.noaksson@jll.se
Linderoth
Maria
M
Gustavsson
Bodil
B
Zebühr
Yngve
Y
Balk
Lennart
L
D000728:Androgens / Q000032:analysis; D000818:Animals; D004703:Endocrine System / Q000187:drug effects; D005260:Female; D012739:Gonadal Steroid Hormones / Q000032:analysis; D007247:Infertility, Female / Q000139:chemically induced / Q000662:veterinary*; D009865:Oocytes / Q000254:growth & development; D010471:Perches / Q000502:physiology*; D011157:Population Dynamics; D012722:Sewage / Q000737:chemistry*; D012995:Solubility; D014865:Waste Disposal, Fluid; D014873:Water Pollutants / Q000506:poisoning*
D000728:Androgens; D012739:Gonadal Steroid Hormones; D012722:Sewage; D014873:Water Pollutants