title: Autologous chondrocyte implantation at the knee using a bilayer collagen membrane with bone graft. A preliminary report.
abstract: Autologous chondrocyte implantation (ACI) is a technique used for the treatment of symptomatic osteochondral defects of the knee. A variation of the original periosteum membrane technique is the matrix-induced autologous chondrocyte implantation (MACI) technique. The MACI membrane consists of a porcine type-I/III collagen bilayer seeded with chondrocytes. Osteochondral defects deeper than 8 to 10 mm usually require bone grafting either before or at the time of transplantation of cartilage. We have used a variation of Peterson's ACI-periosteum sandwich technique using two MACI membranes with bone graft which avoids periosteal harvesting. The procedure is suture-free and requires less operating time and surgical exposure. We performed this MACI-sandwich technique on eight patients, five of whom were assessed at six months and one year post-operatively using the modified Cincinnati knee, the Stanmore functional rating and the visual analogue pain scores. All patients improved within six months with further improvement at one year. The clinical outcome was good or excellent in four after six months and one year. No significant graft-associated complications were observed. Our early results of the MACI-sandwich technique are encouraging although larger medium-term studies are required before there is widespread adoption of the technique.
authors.affiliation: Institute of Orthopaedics, The Royal National Orthopaedic Hospital, Stanmore, Middlesex, England, UK. bartlettw@hotmail.com
authors.forename: W
authors.initials: W
authors.lastname: Bartlett
authors.forename: C R
authors.initials: CR
authors.lastname: Gooding
authors.forename: R W J
authors.initials: RW
authors.lastname: Carrington
authors.forename: J A
authors.initials: JA
authors.lastname: Skinner
authors.forename: T W R
authors.initials: TW
authors.lastname: Briggs
authors.forename: G
authors.initials: G
authors.lastname: Bentley
mesh_terms: D000293:Adolescent; D000328:Adult; D016025:Bone Transplantation / Q000379:methods*; D019902:Chondrocytes / Q000637:transplantation*; D046789:Chondromalacia Patellae / Q000601:surgery*; D024042:Collagen Type I / Q000627:therapeutic use*; D024061:Collagen Type III / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007719:Knee Joint / Q000601:surgery; D008297:Male; D008566:Membranes; D008875:Middle Aged; D010008:Osteochondritis Dissecans / Q000601:surgery*; D011475:Prosthesis Failure; D014182:Transplantation, Autologous
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III