title: Elevation of macrophage-derived chemokine in eosinophilic pneumonia: a role of alveolar macrophages.
abstract: Macrophage-derived chemokine (MDC/CCL22) and thymus-and activation-regulated chemokine (TARC/CCL17) are ligands for CC chemokine receptor 4. Recently, TARC has been reported to play a role in the pathogenesis of idiopathic eosinophilic pneumonia (IEP). The purpose of this study was to evaluate the role of MDC in IEP and other interstitial lung diseases (ILDs). MDC and TARC in the bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay in patients with ILDs and healthy volunteers (HV). We also examined the expression of MDC mRNA in alveolar macrophages (AM) by real-time quantitative reverse transcriptase-polymerase chain reaction. Both MDC and TARC were detected only in BALF obtained from IEP patients. The concentration of MDC was higher than that of TARC in all cases. The level of MDC in IEP correlated with that of TARC. AM from IEP patients expressed a significantly higher amount of MDC than that from HV at the levels of protein and mRNA. MDC in BALF from IEP dramatically decreased when patients achieved remission. These findings suggest that MDC, in addition to TARC, might be involved in the pathogenesis of IEP, and AM play a role in the elevation of MDC in IEP.
authors.affiliation: Department of Internal Medicine and Molecular Therapeutics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Manabe
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Nishioka
authors.forename: Jun
authors.initials: J
authors.lastname: Kishi
authors.forename: Mami
authors.initials: M
authors.lastname: Inayama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Aono
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Ogushi
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Bando
authors.forename: Kenji
authors.initials: K
authors.lastname: Tani
authors.forename: Saburo
authors.initials: S
authors.lastname: Sone
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016022:Case-Control Studies; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011657:Pulmonary Eosinophilia / Q000209:etiology / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C106230:CCL17 protein, human; C515482:CCL22 protein, human; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D019742:Chemokines, CC; D012333:RNA, Messenger