Characterization of a naturally-occurring polymorphism in the UHR-1 gene encoding the putative rat prolactin-releasing peptide receptor.
The rat orphan receptor UHR-1 and its human orthologue, GPR10, were first isolated in 1995. The ligand for this receptor, prolactin-releasing peptide (PrRP), was identified in 1998 by reverse pharmacology and has subsequently been implicated in a number of physiological processes. As supported by its localization and regulation in the hypothalamus and brainstem, we have shown previously that PrRP is involved in energy homeostasis. Here we describe a naturally occurring polymorphism in the UHR-1 gene that results in an ATG to ATA change at the putative translational initiation site. The presence of the polymorphism abolished the binding of 125I PrRP in rat brain slices but did not affect the ability of PrRP to reduce fast-induced food intake. Together this data suggest that PrRP may be exerting its feeding effects through a receptor other than UHR-1.
Ellacott
Kate L J
KL
Faculty of Life Sciences, University of Manchester, 1.124 Stopford Building, Oxford Rd, Manchester M13 9PT, UK.
Donald
Emma L
EL
Clarkson
Paul
P
Morten
John
J
Masters
Dave
D
Brennand
John
J
Luckman
Simon M
SM
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007028:Hypothalamic Hormones / Q000378:metabolism; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D056690:Prolactin-Releasing Hormone; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
D017931:DNA Primers; D007028:Hypothalamic Hormones; D009479:Neuropeptides; C534377:PRLH protein, human; C534379:Prlh protein, rat; D056690:Prolactin-Releasing Hormone; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; C400801:receptor, prolactin-releasing peptide