Mononuclear cell variability and recruitment in non-cytokine-stimulated donors after serial 10-liter leukapheresis procedures.
BACKGROUND
We introduced monitoring of mononuclear cell (MNC) counts to obtain enhanced donor control and a stable quality of MNC products, because there are limited data available about blood donors after serial leukapheresis (LP) procedures.


METHODS
In a prospective paired study, 13 male healthy blood donors underwent 10-L LP procedures performed on two apheresis devices by use of two MNC program settings (COBE Spectra, Gambro BCT, SF 250 vs. SF 500; and AS.TEC 204, Fresenius Hemocare, CP 129 vs. CP 194). Donors' pre- and postdonation MNC counts were analyzed by fluorescence-activated cell sorting.


RESULTS
After each 10-L LP procedure, a transient decline (p < 0.05) of CD14+ monocyte and platelet counts appeared in donors. Loss of donors' CD3+ T cells, CD19+ B cells, and CD16+56+ natural killer (NK) cells during MNC collection was partly compensated by cell recruitment. The MNC recruitment factor (RF) seems to be higher with high-yield MNC program settings. Negative correlations (p < 0.01) were noticed between predonation counts and RFs of CD3+ T cells and CD16+56+ NK cells. Four serial 10-L LP procedures did not result in long lasting MNC depletion for donors.


CONCLUSIONS
MNC recruitment seems to depend on MNC program settings and collected cell yields. Low MNC counts could result in high cell recruitment that may contribute to stable collection results to some degree. Nevertheless, there seems to be a considerable individual variation of MNC recruitment in donors that should be investigated in more detail.
Strasser
Erwin F
EF
Department of Transfusion Medicine and Haemostaseology, University Hospital Erlangen, Erlangen, Germany. erwin.strasser@trans.imed.uni-erlangen.de
Zimmermann
Robert
R
Weisbach
Volker
V
Ringwald
Jürgen
J
Zingsem
Jürgen
J
Eckstein
Reinhold
R
D000328:Adult; D005500:Follow-Up Studies; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation* / Q000379:methods*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D016131:Lymphocyte Subsets / Q000166:cytology; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D011446:Prospective Studies