title: Osmotic stress stimulates the organic osmolyte channel in Xenopus laevis oocytes expressing skate (Raja erinacea) AE1.
abstract: The aim of this study was to determine whether hypo-osmolarity, which activates taurine transport through the volume-sensitive organic osmolyte channel in skate (Raja erinacea) erythrocytes, also activates the organic osmolyte channel activity of skate AE1 (skAE1) expressed in oocytes. When Xenopus laevis oocytes expressing skAE1 were incubated in hypo-osmotic ND 96 (210 mOsm) media, taurine was transported at a significantly higher rate than when incubated in ND 96 (235 mOsm), which is iso-osmotic to Xenopus plasma. Therefore, hypo-osmotic stress is part of the activation mechanism of the organic osmolyte channel in skAE1 expressing oocytes.
authors.affiliation: Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912, USA.
authors.forename: Dana-Lynn T
authors.initials: DL
authors.lastname: Koomoa
authors.forename: Mark W
authors.initials: MW
authors.lastname: Musch
authors.forename: Leon
authors.initials: L
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D015153:Blotting, Western; D005260:Female; D007038:Hypotonic Solutions; D009865:Oocytes / Q000378:metabolism*; D012860:Skates, Fish / Q000235:genetics / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000502:physiology*; D014982:Xenopus laevis / Q000378:metabolism* / Q000502:physiology
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D007038:Hypotonic Solutions; D013654:Taurine