Determination of chloramphenicol residue in fish and shrimp tissues by gas chromatography with a microcell electron capture detector.
A gas chromatography method with microcell electron capture detection was developed for the determination of chloramphenicol residue in fish and shrimp muscle tissues. The tissue samples were extracted with ethyl acetate, defatted with hexane, and derivatized with Sylon BFT [N,O-bis (trimethylsilyl) trifluoroacetamide-trimethylchlorosilane (99 + 1)]. The limit of detection was 0.04 ng/g and the limit of quantitation 0.1 ng/g. Average recoveries were 70.8-90.8% for fish and 69.9-86.3% for shrimp, respectively. The method was validated for the determination of practical samples.
Ding
Shuangyang
S
China Agriculture University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing 100094, Peoples Republic of China.
Shen
Jianzhong
J
Zhang
Suxia
S
Jiang
Haiyang
H
Sun
Zhiwen
Z
D000085:Acetates / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002701:Chloramphenicol / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D033364:Decapoda; D004350:Drug Residues; D004583:Electrons; D005399:Fishes; D005506:Food Contamination; D009132:Muscles / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry
D000085:Acetates; D000900:Anti-Bacterial Agents; D002701:Chloramphenicol; C007650:ethyl acetate; D009584:Nitrogen