title: Tobacco-specific nitrosamines in smokeless tobacco products marketed in India.
abstract: Smokeless tobacco products are a known cause of oral cancer in India. Carcinogenic tobacco-specific nitrosamines in these products are believed to be at least partially responsible for cancer induction, but there have been no recent analyses of their amounts. We quantified levels of 4 tobacco-specific nitrosamines, N'-nitrosonornicotine (NNN), N'-nitrosoanatabine (NAT), N'-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), in 32 products marketed currently in India. Levels of nitrate, nitrite and nicotine were also determined. The highest levels of tobacco-specific nitrosamines were found in certain brands of khaini, zarda and other smokeless tobacco products. Concentrations of NNN and NNK in these products ranged from 1.74-76.9 and 0.08-28.4 microg/g, respectively. Levels of tobacco-specific nitrosamines in gutka were generally somewhat lower than in these products, but still considerably higher than nitrosamine levels in food. Tobacco-specific nitrosamines were rarely detected in supari, which does not contain tobacco, or in tooth powders. The results of our study demonstrate that exposure to substantial amounts of carcinogenic tobacco-specific nitrosamines through use of smokeless tobacco products remains a major problem in India.
authors.affiliation: The Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Irina
authors.initials: I
authors.lastname: Stepanov
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Hecht
authors.forename: Sreevidya
authors.initials: S
authors.lastname: Ramakrishnan
authors.forename: Prakash C
authors.initials: PC
authors.lastname: Gupta
mesh_terms: D002273:Carcinogens; D006801:Humans; D007194:India; D009538:Nicotine / Q000032:analysis; D009566:Nitrates / Q000032:analysis; D009573:Nitrites / Q000032:analysis; D009602:Nitrosamines / Q000032:analysis*; D014030:Tobacco, Smokeless / Q000737:chemistry*
chemical_list: D002273:Carcinogens; D009566:Nitrates; D009573:Nitrites; D009602:Nitrosamines; D009538:Nicotine; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; C008655:N'-nitrosonornicotine