Evaluation of a commercial test based on ligase chain reaction for direct detection of Mycobacterium tuberculosis in respiratory specimens.
A ligase chain reaction DNA amplification method for direct detection of Mycobacterium tuberculosis (Abbott LCx MTB) in respiratory specimens was evaluated. Results from LCx MTB Assay were compared with those from acid fast bacilli smear, culture, and final clinical diagnosis for each patient. A total of 297 respiratory specimens (sputum and bronchial lavage) from 193 patients were tested. The sensitivity, specificity, positive predictive value and negative predictive value of LC vs culture were 92.7%, 93%, 67.8% and 98.7%, respectively. When compared to the clinical final diagnosis, the sensitivity, specificity, PPV and NPV for LCx were 88.9%, 96.8%, 86.5% and 97.4%, respectively. The sensitivity of LCx MTB assay was 75% for smear-negative, culture positive samples. The results indicate that LCx MTB assay is a rapid, simple and valuable technique as a complementary tool for the diagnosis of tuberculosis.
Ribeiro
Fabíola Karla Corrêa
FK
Núcleo de Doenças Infecciosas do Centro Biomédico da Universidade Federal do Espirito Santo, Vitória, ES, Brazil.
Dettoni
Valdério do Valle
Vdo V
Peres
Renata Lyrio
RL
Vinhas
Solange Alves
SA
Có
Tatiana Resende
TR
Dietze
Reynaldo
R
Palaci
Moisés
M
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D005784:Gene Amplification; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D003470:Culture Media; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic