Extrusion of cells with inappropriate Dpp signaling from Drosophila wing disc epithelia.
Decapentaplegic (Dpp) is a signaling molecule that controls growth and patterning of the developing Drosophila wing. Mutant cells lacking Dpp signal transduction have been shown to activate c-Jun amino-terminal kinase (JNK)-dependent apoptosis and to be lost from the wing disc epithelium. These observations have led to the hypothesis that Dpp promotes cell survival by preventing apoptosis. Here, we show that in the absence of JNK-dependent apoptosis, mutant cells lacking Dpp signal transduction can survive; however, they are still lost from the wing disc epithelium. This loss correlates with extensive cytoskeletal changes followed by basal epithelial extrusion. We propose that Dpp promotes cell survival within disc epithelia by affecting cytoskeletal organization.
Shen
Jie
J
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Dahmann
Christian
C
D000199:Actins / Q000032:analysis; D000818:Animals; D017209:Apoptosis; D048430:Cell Shape; D002470:Cell Survival; D002999:Clone Cells / Q000166:cytology / Q000502:physiology; D003599:Cytoskeleton / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D017344:Genes, Insect; D007365:Intercellular Junctions / Q000648:ultrastructure; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008870:Microtubules / Q000648:ultrastructure; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014921:Wings, Animal / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism
D000199:Actins; D029721:Drosophila Proteins; D011956:Receptors, Cell Surface; D016212:Transforming Growth Factor beta; C064196:dpp protein, Drosophila; C090169:tkv protein, Drosophila; D017346:Protein Serine-Threonine Kinases