Transporter and ion channel gene expression after Caco-2 cell differentiation using 2 different microarray technologies.
mRNA expression profiles had previously been measured in Caco-2 cells (human colonic carcinoma cells) using either custom-designed spotted oligonucleotide arrays or Affymetrix GeneChip oligonucleotide arrays. The Caco-2 cells used were from different clones and were examined under slightly different culture conditions commonly encountered when Caco-2 cells are used as a model tissue for studying intestinal transport and metabolism in different laboratories. In this study, we compared gene expression profiles of Caco-2 cells generated with different arrays to assess the validity of conclusions derived from the 2 independent studies, with a focus on changes in transporter and ion channel mRNA expression levels on Caco-2 cell differentiation. Significant changes in expression levels upon differentiation were observed with 78 genes, with probes common to both arrays. Of these, 18 genes were upregulated and 36 genes were downregulated. The 2 arrays yielded discrepant results for 24 genes, showing significant changes upon differentiation. The results from the 2 arrays correlated well for genes expressed above average levels (r = 0.75, P < 0.01, n = 25) and poorly for genes expressed at low levels (r = 0.08, P > 0.05, n = 25). Overall correlation across the 2 platforms was r = 0.45 (P < 0.01) for the 78 genes, with similar results from both arrays. Despite differences in experimental conditions and array technology, similar results were obtained for most genes.
Landowski
Christopher P
CP
Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
Anderle
Pascale
P
Sun
Duxin
D
Sadee
Wolfgang
W
Amidon
Gordon L
GL
D018938:Caco-2 Cells / Q000166:cytology*; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods*
D007473:Ion Channels; D026901:Membrane Transport Proteins