title: Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch.
abstract: Phosphoenolpyruvate (PEP) is a phosphorylated glycolytic intermediate that can penetrate the RBC membrane and be metabolized to 2,3-DPG and ATP. In this study, we evaluated the effects of PEP treatment on canine red blood cells (RBCs) cryopreserved with 12.5% (w/v) HES. RBCs were incubated for 30, 60, and 90 min at 37 degrees C with PEP solution containing 60 mM mannitol, 30 mM sodium chloride, 25 mM glucose, 1 mM adenine and 50 mM PEP (340 m osm/kg), pH 6.0 and then cryopreserved in liquid nitrogen with 12.5% (w/v) HES for 2 weeks. 2,3-DPG and saline stabilities of the PEP treated groups were increased and osmotic fragility indices were significantly decreased compared to the untreated control group. There were no differences in 2,3-DPG levels within the PEP treated groups with different PEP incubation times. These results suggest that PEP treatment may be beneficial for the cryopreservation of canine RBCs with HES.
authors.affiliation: The United Graduate School of Veterinary Sciences, Yamaguchi University, Yoshida, Yamaguchi, Japan. b3646@yamaguchi-u.ac.jp
authors.forename: Heejaung
authors.initials: H
authors.lastname: Kim
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Itamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Une
authors.forename: Munekazu
authors.initials: M
authors.lastname: Nakaichi
authors.forename: Yasuho
authors.initials: Y
authors.lastname: Taura
authors.forename: Sajio
authors.initials: S
authors.lastname: Sumida
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000378:metabolism; D000818:Animals; D001793:Blood Preservation / Q000295:instrumentation* / Q000379:methods*; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism / Q000494:pharmacology; D004285:Dogs; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D009996:Osmotic Fragility; D010728:Phosphoenolpyruvate / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D003451:Cryoprotective Agents; D006895:Hydroxyethyl Starch Derivatives; D019794:2,3-Diphosphoglycerate; D010728:Phosphoenolpyruvate