title: Ultrasonic extraction followed by a novel filtration and clean-up device for screening of some polyphenols in tobaccos.
abstract: A simple and efficient HPLC method using a diode array detector (DAD) was developed for simultaneous determination of six polyphenols in tobaccos. Ultrasonic extraction was employed at 25 degrees C to extract the polyphenols present in tobaccos into anhydrous methanol. A novel filtration device linked to a clean-up cartridge was designed for simultaneous extract filtration and clean-up. Optimized HPLC-DAD analysis, with multi-wavelength detection, was used for determination of the polyphenols. Because the content of some of the polyphenols is too low to be quantified directly, a concentration step was necessary. Anhydrous methanol was employed for extraction of the polyphenols because of its high extraction efficiency and its low boiling point in the concentration step. Using the proposed method, six polyphenols were quantified in tobaccos (Nicotina tobaccum L.).
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.
authors.forename: Xungang
authors.initials: X
authors.lastname: Gu
authors.forename: Jibao
authors.initials: J
authors.lastname: Cai
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Qingde
authors.initials: Q
authors.lastname: Su
mesh_terms: D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D005374:Filtration; D005419:Flavonoids / Q000032:analysis*; D000432:Methanol / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000378:metabolism*; D014465:Ultrasonics
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D000432:Methanol