Evaluation of insulin resistance linkage to rat chromosome 4 in SHR of a Japanese colony.
The spontaneously hypertensive rat (SHR) is a model of human insulin resistance syndrome. Quantitative trait loci for cellular defects in glucose and fatty acid metabolism have been mapped to an overlapping region of rat chromosome (RNO) RNO4 in SHR of the National Institute of Health colony, where a deletion in the Cd36 gene has been implicated as the causative mutation of insulin resistance. The present study has examined the potential presence of RNO4 linkage to a series of metabolic phenotypes in F(2) progeny derived from SHR of a Japanese colony (SHR/Izm) without the Cd36 mutation. Our data demonstrate that 'major' insulin resistance gene(s) are unlikely to exist on RNO4 in SHR/Izm and in vitro phenotypes measured in isolated adipocytes do not cosegregate in the F(2) population studied. Thus, it seems to be difficult to explain the underlying genetic mechanisms of insulin resistance by a single major gene on RNO4.
Watanabe
Takehiro
T
Department of Gene Diagnostics and Therapeutics, Research Institute, International Medical Center of Japan, Japan.
Iizuka
Yoko
Y
Liang
Yi-Qiang
YQ
Inomata
Hyoe
H
Gotoda
Takanari
T
Yanai
Kazuyuki
K
Isobe
Mitsuaki
M
Kato
Norihiro
N
D000818:Animals; D018955:CD36 Antigens / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D007333:Insulin Resistance / Q000235:genetics*; D007564:Japan; D040641:Quantitative Trait Loci / Q000235:genetics*; D051381:Rats; D011918:Rats, Inbred SHR
D018955:CD36 Antigens