Toxicological effects of acrylamide on rat testicular gene expression profile.
Toxicological effects of acrylamide on differential gene expression profile of rat testis were evaluated. Acrylamide induced morphological sperm defects, and decreased sperm concentration in cauda epididymis. Serum testosterone level and Leydig cell viability were also decreased dose-dependently, which resulted in decreased spermatogenesis. Acrylamide-induced histopathological lesions, such as formation of multinucleated giant cells and vacuolation, and numerous apoptotic cells were observed in seminiferous tubules. cDNA microarray analysis revealed that genes related to testicular-functions, apoptosis, cellular redox, cell growth, cell cycle, and nucleic acid-binding were up/down-regulated in testes isolated from acrylamide-treated group (60 mg/kg/day). Acrylamide toxicity appears to increase Leydig cell death and perturb gene expression levels, contributing to sperm defects and various abnormal histopathological lesions including apoptosis in rat testis.
Yang
Hye-Jin
HJ
Department of Biochemistry, College of Veterinary Medicine, Seoul National University, 151-742 Seoul, Republic of Korea.
Lee
Sang-Hyun
SH
Jin
Yong
Y
Choi
Jin-Hyang
JH
Han
Dong-Un
DU
Chae
Chanhee
C
Lee
Mun-Han
MH
Han
Chang-Hoon
CH
D020106:Acrylamide / Q000633:toxicity*; D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004785:Environmental Pollutants / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
D004785:Environmental Pollutants; D020106:Acrylamide