Anticoagulant and antifibrinogenolytic properties of the aqueous extract from Bauhinia forficata against snake venoms.
The aqueous extract from aerial parts of Bauhinia forficata was able to neutralize the clotting activity induced by Bothrops and Crotalus crude venoms. The clotting time, upon human plasma, induced by B. moojeni venom was significantly prolonged. Clotting and fibrinogenolytic activities induced by isolated thrombin-like enzyme from Bothrops jararacussu were totally inhibited after incubation at different ratios. The extract was not able to neutralize the hemorrhagic activity induced by an Bothrops venoms, but it efficiently inhibited the edema induced by Crotalus durissus terrificus venom and isolated PLA2s. In addition, it did not inhibited the phospholipase A2 activity of Bothrops snake venoms. Interaction studies between Bauhinia forficata extract and snake venoms, when analyzed by SDS-PAGE, did not reveal any apparent degradation of the venom proteins. This extract is a promising source of natural inhibitors of serine-proteases involved in blood clotting disturbances induced by snake venoms.
Oliveira
Clayton Z
CZ
Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP, 14096-900 Ribeirão Preto, SP, Brazil.
Maiorano
Victor A
VA
Marcussi
Silvana
S
Sant'ana
Carolina D
CD
Januário
Ana H
AH
Lourenço
Miriam V
MV
Sampaio
Suely V
SV
França
Suzelei C
SC
Pereira
Paulo S
PS
Soares
Andreimar M
AM
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D000933:Antifibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D036481:Bauhinia / Q000737:chemistry / Q000378:metabolism*; D003435:Crotalid Venoms / Q000009:adverse effects / Q000037:antagonists & inhibitors; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012910:Snake Venoms / Q000009:adverse effects / Q000037:antagonists & inhibitors* / Q000737:chemistry; D014867:Water / Q000737:chemistry / Q000494:pharmacology
D000925:Anticoagulants; D000933:Antifibrinolytic Agents; D003435:Crotalid Venoms; D010936:Plant Extracts; D012910:Snake Venoms; D014867:Water; C030961:botrocetin