Protective effects of Rheum tanguticum polysaccharide against hydrogen peroxide-induced intestinal epithelial cell injury.
OBJECTIVE
To describe the effect of Rheum tanguticum polysaccharide (RTP) on hydrogen peroxide-induced human intestinal epithelial cell injury.


METHODS
Hydrogen peroxide (100 micromol/L) was introduced to induce human intestinal epithelial cell injury. Cells were pretreated with RTP (30,100,300 microg/mL) for 24 h before exposure to hydrogen peroxide. Cell viability was detected by MTT assay and morphological observation. Acridine orange staining and flow cytometry were performed to assess cell apoptosis. Lactate dehydrogenase (LDH) activity, production of malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured by spectrophotometry with corresponding assay kits.


RESULTS
Following exposure to H(2)O(2), a marked decrease in cell survival and SOD activity, increased production of MDA, LDH leakage and cell apoptosis were found. Pretreatment of the cells with RTP could significantly elevate cell survival, SOD activity and decrease the level of MDA, LDH activity and cell apoptosis.


CONCLUSIONS
RTP may have cytoprotective and anti-oxidant effects against H(2)O(2)-induced intestinal epithelial cell injury by inhibiting cell apoptosis and necrosis. This might be one of the possible mechanisms of RTP for the treatment of ulcerative colitis in rats.
Liu
Lin-Na
LN
Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
Mei
Qi-Bing
QB
Liu
Li
L
Zhang
Feng
F
Liu
Zhen-Guo
ZG
Wang
Zhi-Peng
ZP
Wang
Ru-Tao
RT
D002460:Cell Line; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D012250:Rheum / Q000737:chemistry*
D016877:Oxidants; D010936:Plant Extracts; D011134:Polysaccharides; D020011:Protective Agents; D006861:Hydrogen Peroxide