Sequential UV-biological degradation of polycyclic aromatic hydrocarbons in two-phases partitioning bioreactors.
A method based on UV-irradiation in organic solvent followed by transfer of the remaining pollutants into silicone oil for subsequent biodegradation in a biphasic system inoculated with a phenanthrene degrading Pseudomonas sp. was tested for the treatment of various mixtures of PAHs. Acetone was first selected as the most suitable solvent compared to methanol, acetonitrile and silicone oil for the removal of pyrene and phenanthrene. The sequential treatment was then applied to the treatment of a mixture of fluorene, phenanthrene, anthracene, fluoranthrene, pyrene, benzo(a)anthracene and benzo(a)pyrene in acetone. These compounds were photodegraded in the following order of initial removal rates (mg l(-1) d(-1)): benzo(a)pyrene (7.8) > anthracene (5.0) > benzo(a)anthracene (2.5) > fluoranthrene (1.8) > pyrene (1.5) > phenanthrene (1.2) > fluorene (0.2). UV-treatment allowed complete removal of, anthracene, benzo(a)anthracene and benzo(a)pyrene and removals of 63% of pyrene and 37% of fluorene after 434 h or irradiation. The subsequent biological treatment removed the remaining phenanthrene and fluorene by 100% and 90%, respectively, after 790 h of cultivation. Although less efficient due to the presence of interfering compounds, the UV-biological treatment of a soil extract allowed a 63% removal of the seven PAHs named above. Microbial growth did not occur when the pollutants were directly supplied to the microorganism showing that biphasic systems reduced the toxicity effects cause by mixtures of PAHs at high concentrations. This study demonstrates the potential of selective UV treatment of high molecular weight PAHs followed by biological treatment of the low molecular weight species in biphasic systems.
Guieysse
Benoit
B
Department of Biotechnology, Lund University, P.O. Box 124, S-221 00 Lund, Sweden. benoit.guieysse@biotek.lu.se
Viklund
Gunilla
G
D000704:Analysis of Variance; D001673:Biodegradation, Environmental / Q000528:radiation effects; D019149:Bioreactors / Q000382:microbiology*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004787:Environmental Pollution / Q000517:prevention & control*; D010782:Photolysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D012827:Silicone Oils; D014466:Ultraviolet Rays
D011084:Polycyclic Aromatic Hydrocarbons; D012827:Silicone Oils