Asanoa iriomotensis sp. nov., isolated from mangrove soil.
During a study of the distribution of actinomycetes in a mangrove zone, a strain forming spore chains borne on the tip of short sporophores arising directly from the agar surface was isolated from soil. The isolate contained glutamic acid, glycine, alanine and meso-diaminopimelic acid as cell-wall amino acids, menaquinone MK-10(H(6), H(8)), fatty acid type 2d and xylose in the whole-cell hydrolysate. The 16S rRNA gene sequence of the isolate formed a monophyletic cluster with the members of the genus Asanoa in the family Micromonosporaceae. On the basis of morphological and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA hybridization, a novel species of the genus Asanoa is proposed for strain TT 97-02(T) (=NBRC 100142(T)=DSM 44745(T)), Asanoa iriomotensis sp. nov.
Tamura
Tomohiko
T
Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0812, Japan.
Sakane
Takeshi
T
Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0812, Japan.
D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D007564:Japan; D008848:Micromonosporaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D043982:Rhizophoraceae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S