Identification of two fungicide degrading Pseudomonas species by gas chromatography of cellular fatty acids.
Microorganisms can be identified in many ways. Conventional methods rely on the expression of certain properties that are usually mediated directly by enzyme activity. Extension of this approach to include numerical identification or automated systems to analyze results often strengthens conclusions. Identification of bacterial species using morphological characters and biochemical tests is often difficult and time consuming. Immunodiagnostic and nucleotide hybridization techniques have improved sensitivity, specify, precision, and ease of testing. Chemotaxonomy is also precise and can result in the definition of highly discriminatory properties. Cellular Fatty Acids (CFA) analyses fall into this category. One of the most convenient methods for the identification of fatty acids in bacterial cells is by gas-liquid chromatography of their methyl esters prepared from phospholipids, total lipids, or other lipid fractions. Two bacterial strains from Bahar Yossof. Al-Fayiurn governorates, Egypt tentatively identified as a species of pseudomonas by virtue of its physiological and biochemical characteristics. Confirmation of this identification was carried out using fatty acids profile analysis.
Zayed
M E
ME
Botanic & Microbiological Department, Faculty of Science, Al-Azhar University, Madient Nasr, Cairo, Egypt.
D001673:Biodegradation, Environmental; D002849:Chromatography, Gas / Q000379:methods; D005227:Fatty Acids / Q000032:analysis*; D005659:Fungicides, Industrial / Q000493:pharmacokinetics*; D007202:Indicators and Reagents; D008745:Methylation; D011549:Pseudomonas / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure
D005227:Fatty Acids; D005659:Fungicides, Industrial; D007202:Indicators and Reagents