Effect of tamoxifen on pituitary vasopressin level.
BACKGROUND
Estrogens modulate the release of Arginine Vasopressin (AVP). There is an increased level of AVP in plasma and pituitary under osterogen hormones.


METHODS
Ten rats were experimental and ten were controls. The ten experimental rats were injected tamoxifen daily subcutaneously for three days in does of 1 mg/kg body weight in 0.1 ml vegetable oil base. The ten control rats were given 0.1 ml of vegetable oil vehicle only subcutaneously. On the morning of the fourth day all the twenty rats were decapitated. Blood was centrifuged at 2500 rev/min for 15 min at 4 degrees C. Plasma was stored at -20 degrees C for assay of AVP. Pituitary gland was removed and homogenized in a test tube containing 1 ml of 0.2 molar acetic acid & stored at -20 degrees C for analysis of pituitary AVP. Uteri of all rats were removed by careful dissection & weighed.


RESULTS
Uterine weight and plasma AVP levels were decreased but the pituitary AVP level was unaffected.


CONCLUSIONS
Under Tamoxifen (anti-estrogen) effect the plasma vasopressin decreased but the level in the posterior was not affected. The effect of osterogen on Hypothalamo Pituitary axis (HPA is measurable in the hypothalamus therefore probably anti-oestrogenic effect would operate more at hypothalamic level rather than posterior pituitary. This needs further investigation at the Hypothalamus level.
Kundi
Salma Aslam
SA
Ayub Medical College, Abbottabad. salmakundi@hotmail.com
Forsling
Mary
M
Kleimestur
Halluck
H
Wazir
Saleem
S
Khan
Taj Muhammad
TM
D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis / Q000378:metabolism*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D007279:Injections, Subcutaneous; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism; D011336:Probability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D013629:Tamoxifen / Q000494:pharmacology*
D013629:Tamoxifen; D001127:Arginine Vasopressin