Cholesteryl oligoethyleneglycol glycosides: fluidizing effect of their embedment into phospholipid bilayers.
Glycosides of cholesteryl oligoethyleneglycols have been synthesized and embedded in liposome bilayers. Several methods as steady-state fluorescence polarization, differential scanning calorimetry, zeta potential, and agglutination have been used to describe the physicochemical outcome of the incorporation of these synthetic glycolipids within phospholipid layers. From calorimetry and fluorescence experiments, it is apparent that the glycolipids decrease the transition temperature of the bilayers in a more important extent than cholesterol. Furthermore, the thickness of the aqueous layer fixed around the liposome is independent of the presence of glycolipids, suggesting that their hydrophilic parts (tetraethyleneglycol spacer and sugar moiety) are not completely extended towards the aqueous phase. Nevertheless, an important and specific interaction has been observed between such glycosylated liposomes and vegetal lectins.
Bardonnet
Pierre-Louis
PL
Laboratoire de Pharmacie Galénique Industrielle, EA 3741, ISPB, Université Claude Bernard, Lyon I, 8 av. Rockefeller, 69373 Lyon, France.
Faivre
Vincent
V
Pirot
Fabrice
F
Boullanger
Paul
P
Falson
Françoise
F
D002152:Calorimetry, Differential Scanning; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000737:chemistry*; D005454:Fluorescence Polarization; D006027:Glycosides / Q000737:chemistry*; D037102:Lectins / Q000494:pharmacology; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008560:Membrane Fluidity / Q000187:drug effects; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry* / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology
D006027:Glycosides; D037102:Lectins; D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; C118255:polyethylene glycol-cholesterol; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D002784:Cholesterol