Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray.
We describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-gamma at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format.
Kim
Min-Gon
MG
Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea.
Shin
Yong-Beom
YB
Jung
Jin-Mi
JM
Ro
Hyeon-Su
HS
Chung
Bong Hyun
BH
D002384:Catalysis; D011232:Chemical Precipitation; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000032:analysis; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D007371:Interferon-gamma; D006735:Horseradish Peroxidase