The revelation of expressing region in the processed ceruloplasmin gene in human genome by biocomputational and biochemical methods.
ANNOTATION: Translation in all open reading frames (ORF) of human ceruloplasmin (Cp) pseudogene revealed the only translating sequence of 984 nucleotides. The amino acid sequence contains a signal peptide for mitochondrial protein import at N-terminus. The predicted protein without taking the signal peptide into consideration has 92% identity to the corresponding Cp fragment. It contains 20 amino acid substitutions, 8 of them are significant. There is His-X-His motif in the center of a molecule that is typical for copper containing oxidases. Potential copper-binding site appears as a result of the substitution P923H along human Cp sequence. Cp pseudogene transcription product was found in the cultured human cell lines HepG2 and HuTu 80 using RT-PCR strategy. Cp polypeptides with molecular weight of nearly 30 kDa were found in mitochondria of HuTu 80 cells. The possible biological role of mitochondrial Cp is under discussion.
Platonova
N A
NA
Research Institute of Experimental Medicine, 197376, Ac. Pavlov str. 12, St. Petersburg, Russia. nataliap2003@mail.ru
Vasin
A V
AV
Klotchenko
S A
SA
Tsymbalenko
N V
NV
Puchkova
L V
LV
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002570:Ceruloplasmin / Q000737:chemistry* / Q000235:genetics*; D019295:Computational Biology; D003201:Computers; D015894:Genome, Human; D006801:Humans; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011544:Pseudogenes / Q000235:genetics*; D016415:Sequence Alignment
D002570:Ceruloplasmin