title: The differential tissue distribution of the citrus flavanone naringenin following gastric instillation.
abstract: Citrus flavonoids have been investigated for their biological activity, with both anti-inflammatory and -carcinogenic effects being reported. However, little information is known on the bioavailability of these compounds in vivo. The objectives of this study were to determine the tissue distribution of naringenin after gastric gavage of [3H]-naringenin to rats. Unlabelled naringenin was also used to quantify the levels of naringenin and its major metabolites in tissues and eliminated in the urine and faeces. Significant radioactivity was detected in the plasma as well as all tissues examined 2h post-gavage. After 18h, higher levels of radioactivity were retained in plasma and tissues (55% of the administered radioactivity). Investigation of the nature of metabolites, using unlabelled naringenin, revealed that the glucuronides were the major components in plasma, tissues and urine, in addition to the colonic metabolite 3-(4-hydroxyphenyl) propionic acid, detected in the urine. The aglycone was the form extensively retained in tissues after 18h post-gavage. Total identified metabolites detected after 18h in most tissues were only 1-5% of the levels detected after 2h. However, the brain, lungs and heart retained 27, 20 and 11%, respectively, relative to the total metabolites detected at 2h. While radioactive detection suggests increased levels of breakdown products of naringenin after 18 h versus 2 h, the products identified using unlabelled naringenin are not consistent with this, suggesting that a predominant proportion of the naringenin breakdown products at 18 h are retained as smaller decomposition molecules which cannot yet be identified.
authors.affiliation: Antioxidant Research Group, Wolfson Centre for Age-Related Diseases, GKT School of Biomedical Sciences, King's College, London, SE1 9RT UK.
authors.forename: Manal Abd
authors.initials: MA
authors.lastname: El Mohsen
authors.forename: Joanne
authors.initials: J
authors.lastname: Marks
authors.forename: Gunter
authors.initials: G
authors.lastname: Kuhnle
authors.forename: Catherine
authors.initials: C
authors.lastname: Rice-Evans
authors.forename: Kevin
authors.initials: K
authors.lastname: Moore
authors.forename: Glenn
authors.initials: G
authors.lastname: Gibson
authors.forename: Edward
authors.initials: E
authors.lastname: Debnam
authors.forename: S Kaila
authors.initials: SK
authors.lastname: Srai
mesh_terms: D000818:Animals; D001682:Biological Availability; D002957:Citrus / Q000737:chemistry; D044950:Flavanones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D007322:Instillation, Drug; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D013270:Stomach; D014018:Tissue Distribution
chemical_list: D044950:Flavanones; C005273:naringenin