Changes in pH increase perfusion pressure of coronary arteries in the rat.
Stricture of coronary arteries is closely related to ischemic heart disease. The purpose of this study was to examine whether changes in pH caused contraction of rat coronary arteries, as determined using Langendorff perfused hearts. Changing the pH of the perfusate increased perfusion pressure as an indication of the contractile state of coronary arteries. Alkaline pH-induced increase of perfusion pressure in Wistar Kyoto rats (WKY) was almost identical to that of spontaneously hypertensive rats (SHR), whereas acidic pH-induced increase in SHR was much greater than that in WKY. Acidic pH-induced increase in perfusion pressure was inhibited by verapamil, cromakalim, and adenosine. Feeding WKY with N(G)-nitro-L-arginine resulted in hypertension followed by enhanced acidic pH-induced increase in perfusion pressure. These results suggest that acidic-pH induced contraction of rat coronary arteries is caused by Ca(2+) influx through voltage-dependent Ca(2+) channels and the contraction is enhanced by hypertension.
Horai
Yasushi
Y
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai.
Furukawa
Ken-Ichi
K
Iwata
Shingo
S
Ohizumi
Yasushi
Y
D000138:Acidosis / Q000139:chemically induced / Q000503:physiopathology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D003331:Coronary Vessels / Q000187:drug effects*; D019806:Cromakalim / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007537:Isometric Contraction / Q000187:drug effects / Q000235:genetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000503:physiopathology; D009200:Myocardial Contraction / Q000187:drug effects / Q000235:genetics; D010477:Perfusion; D010646:Phentolamine / Q000494:pharmacology; D011312:Pressure; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017208:Rats, Wistar; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D014665:Vasodilator Agents; D019806:Cromakalim; D006493:Heparin; D014700:Verapamil; D010646:Phentolamine