Mechanical and electrophysiological effects of isoprostanes on bronchial arterial smooth muscle.
We investigated the mechanical and electrophysiological responses of human and porcine bronchial arterial smooth muscle to isoprostanes (metabolites of membrane lipid peroxidation). These evoked a constrictor response which was sensitive to blockade of thromboxane receptors, as well as to a non-specific tyrosine kinase inhibitor and an inhibitor of Rho-kinase. The patch clamp technique was used to characterize the K+ and Ca2+ currents in these tissues, and to show that isoprostanes caused a brief enhancement of K+ currents followed by prolonged and marked suppression of the same.
Janssen
L J
LJ
Father Sean O'Sullivan Research Centre, Firestone Institute for Respiratory Health, St. Joseph's Hospital, Department of Medicine, McMaster University, Hamilton, Ontario, Canada. janssenl@mcmaster.ca
Tazzeo
T
T
Miller
J
J
D000818:Animals; D001981:Bronchial Arteries / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D004594:Electrophysiology; D006801:Humans; D028421:Isoprostanes / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D013552:Swine
D028421:Isoprostanes; D011188:Potassium; D002118:Calcium