Optimized synthesis of functionalized fluorescent oligodeoxynucleotides for protein labeling.
Phthalimido-alkanol solid supports were rapidly prepared from solid supported phthalic anhydride and amino alcohol condensation induced by microwaves. These supports were used to synthesize 5'-fluorescent 3'-aminoalkyl oligodeoxynucleotides allowing a two-step deprotection necessary to avoid aminolink alkylation. After conversion into an NHS derivative using dissuccinimidyl suberate and an optimized isolation, they were conjugated with a protein.
Goussu
Charles
C
Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM II, Université de Montpellier II, CC008, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
Vasseur
Jean-Jacques
JJ
Bazin
Hervé
H
Trinquet
Eric
E
Maurin
Fabrice
F
Morvan
François
F
D002232:Carbocyanines; D005456:Fluorescent Dyes; D018796:Immunoconjugates / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D002232:Carbocyanines; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D009838:Oligodeoxyribonucleotides; D011506:Proteins; C085321:cyanine dye 5