[Studies on application and mechanism of energy transfer system of acridine orange (AO)-rhodamine B (RB) dimer in the determination of DNA].
Not only dye monomer is the form of energy transfer but also dye dimer is the effective form of energy transfer. In this work, the energy transfer system of acridine orange (AO)-rhodamine B (RB) dimer was built for the determination of DNA as fluorescence probe. At the same time, the mechanism was also considered. Under the optimum experimental conditions, the linear range of this assay was 0.33-1.33 mg x L(-1) for fish sperm DNA and 0.33-3.33 mg x L(-1) for calf thymus DNA, the detection limit was 1.63 x 10(-3) mg x L(-1) for fish sperm DNA and 1.52 x 10(-3) mg x L(-1) for calf thymus DNA, respectively. The RSD for the determination of 1.00 mg x L(-1) DNA was 2.40% for fsDNA and 2.00% for ctDNA, respectively. The method was used for the determination of DNA in synthetic samples with satisfied results.
Gao
Feng
F
College of Chemistry and Material Science, Anhui Normal University, Wuhu 241000, China.
Zhu
Chang-qing
CQ
Wang
Lun
L
D000165:Acridine Orange / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D057230:Limit of Detection; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D055672:Static Electricity
D005456:Fluorescent Dyes; D012235:Rhodamines; D004247:DNA; C427203:calf thymus DNA; D000165:Acridine Orange; C029773:rhodamine B