Characteristics of trapping various organophosphorus pesticides with a ferritin reactor of shark liver (Sphyrna zygaena).
A reactor is composed of liver ferritin of Sphyrna zygaena (SZLF) and an oscillating bag. A reactive procedure for trapping various organphosphorus pesticides (OPs) with the SZLF reactor in the flowing water is described in detail, showing the maximal trapping numbers of 28 +/-1.0 dichlorovos/SZLF, 42 +/- 1.0 dimethoate/SZLF, and 55 +/- 1.0 methamido-phos/SZLF determined by a improved spectrophotometric method in 12 h. In addition, it is found that the OP numbers trapped by the reactor increase along with the incubation time and its concentration increment in the flowing water (or seawater), respectively. This trapping capacity is considered to depend on the composition of amino acids on the surface of the ferritin shell interior rather than the available volume within the shell. A novel pathway for trapping various OPs with the ferritin is suggested in reference to unstable characteristics of the protein subunits. We claim that the ferritin reactor will be employed to monitor the contamination level of various OPs in the flowing water continuously.
Huang
He-Qing
HQ
Department of Biochemistry and Biotechnology, School of Life Sciences, Key Laboratory for Chemical Biology of Fujian Province, China. hqhuang@xmu.edu.cn
Xiao
Zhi-Qun
ZQ
Lin
Qing-Mei
QM
Chen
Ping
P
D000818:Animals; D004006:Dichlorvos / Q000032:analysis; D004058:Diffusion; D005293:Ferritins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis*; D009946:Organothiophosphorus Compounds / Q000032:analysis; D010575:Pesticides / Q000032:analysis*; D012754:Sharks / Q000378:metabolism*; D014873:Water Pollutants / Q000032:analysis*
D009943:Organophosphorus Compounds; D009946:Organothiophosphorus Compounds; D010575:Pesticides; D014873:Water Pollutants; D004006:Dichlorvos; C014655:methamidophos; D005293:Ferritins