title: Histological study of the effect of some irrigating solutions on bacterial endotoxin in dogs.
abstract: The aim of this study was to evaluate, histopathologically, the effectiveness of mechanical preparation of root canals using different irrigating solutions in dog teeth filled with LPS after pulpectomy. A total of 120 root canals of 6 mongrel dogs were filled with a solution of LPS after pulpectomy. The irrigating solutions used were saline, 1, 2.5, and 5% sodium hypochlorite, and 2% chlorhexidine. No irrigation was used in the control group. The animals were sacrificed after 60 days and the teeth were fixed and demineralized. Subsequently, serial 6-microm sections were stained with hematoxylin and eosin and Mallory's trichrome for histopathological analysis and Brown-Brenn for verification of bacterial contamination. Analysis showed that the inflammatory infiltrate was statistically less intense in the groups in which the root canals were irrigated with 5% sodium hypochlorite and 2% chlorhexidine. However, none of the irrigating solutions completely inactivated the harmful effects of LPS. Mechanical preparation associated with different irrigating solutions did not completely inactivate LPS.
authors.affiliation: Department of Clinical Pediatrics, School of Dentistry of Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil. msantos@forp.usp.br
authors.forename: Léa Assed Bezerra da
authors.initials: LA
authors.lastname: Silva
authors.forename: Mario Roberto
authors.initials: MR
authors.lastname: Leonardo
authors.forename: Sada
authors.initials: S
authors.lastname: Assed
authors.forename: Mário
authors.initials: M
authors.lastname: Tanomaru Filho
mesh_terms: D000818:Animals; D002710:Chlorhexidine / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000187:drug effects; D004285:Dogs; D005260:Female; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008297:Male; D010486:Periapical Tissue / Q000187:drug effects; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation; D012965:Sodium Chloride / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric
chemical_list: D008070:Lipopolysaccharides; D012388:Root Canal Irrigants; D012965:Sodium Chloride; D012973:Sodium Hypochlorite; D002710:Chlorhexidine