Expression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats.
Ciliary neurotrophic factor (CNTF) is known as a neuro-survival factor in the developing and developed CNS, as well as in the CNS following injury. However, little is known about the expression of CNTF or that of its receptor (CNTFR-alpha) in cases of intracerebral hemorrhage (ICH). We investigated the temporal and spatial profiles of CNTF and CNTFR-alpha expression using a collagenase-induced ICH rat model. CNTF expression was up-regulated from the day following ICH induction and reached a peak level at 5 to 14 days, with increased expression observed in brain tissue surrounding the hematoma lesion and white matter structures in association with astroglial proliferation. Further, CNTFR-alpha was transiently expressed in the cerebral cortex surrounding the hematoma, with a peak at 5 days. Administration of exogenous CNTF into the lesion following initiation of ICH resulted in a prolonged expression of CNTFR-alpha on cortical neurons neighboring the hematoma. Our findings suggest differential regulation of CNTF and CNTFR-alpha, and the possibility of a therapeutic strategy using CNTF administration for ICH.
Yokota
Hiroshi
H
Department of Neurosurgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
Yoshikawa
Masahide
M
Hirabayashi
Hidehiro
H
Nakase
Hiroyuki
H
Uranishi
Ryunosuke
R
Nishimura
Fumihiko
F
Sugie
Yuko
Y
Ishizaka
Shigeaki
S
Sakaki
Toshisuke
T
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D020934:Ciliary Neurotrophic Factor / Q000096:biosynthesis*; D008297:Male; D021122:Protein Subunits / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020801:Receptor, Ciliary Neurotrophic Factor / Q000096:biosynthesis*; D015854:Up-Regulation / Q000502:physiology
D020934:Ciliary Neurotrophic Factor; D021122:Protein Subunits; D020801:Receptor, Ciliary Neurotrophic Factor