[Azoic color reaction of flavonoids and their analytical application].
In NH4Cl-NH3 H2O buffer solution of pH 10.4, diazo salt of p-sulfodiazoben-zene chloride reacts with rutin, morin, genistin, quercetin, hesperidin, baicalin and daidzin to form color azo-compounds. These azo-compounds have an absorption maximum at 430 nm except for baicalin azo-compound, which has an absorption maximum at 380 nm. The sensitivity of azoic color reaction is reductive in the order of above flavonoids and the molar absorptivity is maximum for rutin and is minimal for daidzin among these azo-compounds, i. e. 3.28 x 10(4) and 8.3 x 10(2) L x mol(-1) x cm(-1) respectively. The proposed spectrophotometry has been applied to th determination of flavonoids in sophora seed and soybean cake. The RSDs of the method were found to be in the range of 0.75-4.48%, and the recoveries were in the range of 99.3%-105.0%.
Yu
Qian
Q
Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090, China.
Chen
Xiao-Kang
XK
Luo
Zong-Ming
ZM
Yang
Chi
C
Mai
Hua-Kun
HK
D001391:Azo Compounds / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D003116:Color; D005419:Flavonoids / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry; D012431:Rutin / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D047309:Flavones
D001391:Azo Compounds; D005419:Flavonoids; D011794:Quercetin; D012431:Rutin; C038044:baicalin; C008548:morin; D047309:Flavones