[Study on the differentially expressed genes in bone tissue of ovariectomized rats after intervention with Chinese herbs].
OBJECTIVE
To explore the therapeutic effect and mechanism of jiangu granule (JGG) on postmenopausal osteoporosis.


METHODS
Differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.


RESULTS
Ten differentially expressed gene fragments were found, 9 of them, after cloning, sequencing and BLAST searching, were proved to be matched with highly homologous sequences in Genebank. Among them, two were known proteins: hyaluronan-mediated motility receptor (RHAMM) and ATPase, Na+, K+ transporting beta 3 polypeptide (ATP1b3). It was confirmed by semi-quantitative RT-PCR that these two gene fragments could be down- and up-regulated by JGG respectively.


CONCLUSIONS
The therapeutic effect of JGG in treating postmenopausal osteoporosis might be related with its regulation on the two kinds of protein, RHAMM and ATP1b3.
Ma
Yu-ying
YY
Institute of Integrated Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040.
Zhang
Xin-min
XM
Li
Shi-feng
SF
Gu
Yi-feng
YF
Chen
Wei-hua
WH
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D010024:Osteoporosis / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D010052:Ovariectomy; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal