title: Mechanical deformation inhibits IL-10 and stimulates IL-12 production by mouse calvarial osteoblasts in vitro.
abstract: The skeleton is continuously remodelled throughout life, a process that is orchestrated by cells of the osteoblast lineage. Remodelling involves a complex network of cell-cell signalling involving systemic hormones, locally produced cytokines, growth factors and the mechanical environment of the cells. Here, we report on the effect of mechanically-induced strain on the synthesis by mouse calvarial osteoblasts in monolayer culture of IL-10 and IL-12, two cytokines that inhibit osteoclast formation in bone marrow cultures; IL-10 also suppresses osteoblast differentiation suggesting a role for both cytokines in bone physiology. A tensile strain was applied to the cells intermittently for 6s, every 90s, for 2-96h. After 2-h culture, supernatants from deformed cells contained significantly less IL-10 than control cultures. In contrast, mechanical deformation had a stimulatory effect on IL-12 synthesis; however, by 48h both had returned to control levels. These data suggest that IL-10 and IL-12 can be added to the growing list of mechanical stress-responsive genes. The down-regulation of IL-10 and stimulation of IL-12 further suggests that the initial response of the cells to mechanical deformation was an osteogenic one.
authors.affiliation: Health Science Department/ Building H-104, Universidad Autonóma Metropolitana, Calzada del Hueso 1100, Villa Quietud 04960 Mexico City, Mexico. salgarlop@hotmail.com
authors.forename: S
authors.initials: S
authors.lastname: García-López
authors.forename: M C
authors.initials: MC
authors.lastname: Meikle
authors.forename: R E
authors.initials: RE
authors.lastname: Villanueva
authors.forename: L
authors.initials: L
authors.lastname: Montaño
authors.forename: F
authors.initials: F
authors.lastname: Massó
authors.forename: V
authors.initials: V
authors.lastname: Ramírez-Amador
authors.forename: R
authors.initials: R
authors.lastname: Bojalil
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016723:Bone Remodeling; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D016753:Interleukin-10 / Q000032:analysis / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000276:immunology / Q000502:physiology*; D012212:Rheology; D012886:Skull; D013314:Stress, Mechanical
chemical_list: D016753:Interleukin-10; D018664:Interleukin-12