Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs.
Five strains of an unusual Gram-negative, catalase-positive, oxidase-positive, coccobacillus-shaped bacterium isolated from the lungs and heart of pigs with pneumonia and pericarditis were characterized by phenotypic and molecular genetic methods. On the basis of cellular morphology and biochemical criteria, the isolates were tentatively assigned to the family Neisseriaceae, although they did not appear to correspond to any recognized genus or species. Comparative 16S rRNA gene sequencing showed that the five unidentified strains were phylogenetically highly related to each other and represent a hitherto unknown subline within the family Neisseriaceae. On the basis of both phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from pigs be classified as a novel genus and species within the family Neisseriaceae, for which the name Uruburuella suis gen. nov., sp. nov. is proposed. The type strain of U. suis is 1258/02(T) (=CCUG 47806(T)=CECT 5685(T)).
Vela
A I
AI
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Collins
M D
MD
School of Food Biosciences, University of Reading, Reading RG6 6AP, UK.
Lawson
P A
PA
School of Food Biosciences, University of Reading, Reading RG6 6AP, UK.
García
N
N
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Domínguez
L
L
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Fernández-Garayzábal
J F
JF
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D006321:Heart / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016870:Neisseriaceae Infections / Q000382:microbiology / Q000662:veterinary; D010493:Pericarditis / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D010802:Phylogeny; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S