[Expression of IL-23 gene in murine colon carcinoma cells transduced with recombinant retrovirus and its anti-tumor activity].
OBJECTIVE
To obtain murine colon carcinoma cell lines stably transducted by recombinant retrovirus encoding mIL-23 gene.


METHODS
The retrovirus vector was used to transduce the mIL-23 gene into murine colon carcinoma cells (Colon26) and stable clones expressing mIL-23 (Colon26/IL-23) were obtained by screening with G418. The expression of mIL-23 gene was detected by PCR and RT-PCR. The amount of mIL-23 secreted from Colon26/IL-23 cells and IFN-gamma production by murine splenocytes induced by mIL-23 were determined by ELISA. MTT colorimetry was used to assess the in vitro growth of Colon26/IL-23 cells. BALB/c mice were injected s.c. with Colon26/IL-23 cells to observe their tumorigenicity.


RESULTS
Colon26/IL-23 cells were set up. Colon26/IL-23 could secrete mIL-23 which could induce the IFN-gamma production by murine splenocytes. The growth of Colon26/IL-23 cells was similar to that of Colon26 cells in vitro. But the tumorigenicity of Colon26/IL-23 cells was lower than that of Colon26 cells in vivo.


CONCLUSIONS
Colon26/IL-23 cells secreting mIL-23 were obtained and IL-23 had anti-tumor activity.
Hao
Jing-sheng
JS
Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China. jshhao@yahoo.com.cn
Shan
Bao-en
BE
Li
Qiao-xia
QX
Li
Qi-liang
QL
Fu
Xiao-mei
XM
D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D005818:Genetic Engineering; D016678:Genome / Q000235:genetics; D007371:Interferon-gamma / Q000378:metabolism; D053759:Interleukin-23 / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D014161:Transduction, Genetic
D053759:Interleukin-23; D012333:RNA, Messenger; D007371:Interferon-gamma