title: Particle separation using ultrasound can be used with human shed mediastinal blood.
abstract: BACKGROUND
  Shed mediastinal blood collected by cardiotomy suction has been shown to be a large contributor to lipid microemboli ending up in different organs. The aim of this study was to test the separation efficiency on human shed blood of a new separation method developed to meet this demand.
  
  
  METHODS
  Shed mediastinal blood collected from the pericardial cavity of 13 patients undergoing cardiac surgery with cardiopulmonary bypass was collected. The blood was processed in an eight-channel parallel PARSUS separator, and separation efficiency was determined.
  
  
  RESULTS
  Erythrocyte recovery, in terms of a separation ratio, varied between 68% and 91%. Minor electrolyte changes took place, where levels of sodium increased and levels of potassium and calcium decreased.
  
  
  CONCLUSIONS
  This study demonstrates that PARSUS technology can be used on human shed mediastinal blood with good separation efficiency. The technology is, thereby, suggested to have future clinical relevance.
authors.affiliation: Department of Cardiothoracic Surgery, Center for Heart and Lung Disease, Lund University Hospital, Lund, Sweden. henrik.jonsson@thorax.lu.se
authors.forename: Henrik
authors.initials: H
authors.lastname: Jönsson
authors.forename: Andreas
authors.initials: A
authors.lastname: Nilsson
authors.forename: Filip
authors.initials: F
authors.lastname: Petersson
authors.forename: Mats
authors.initials: M
authors.lastname: Allers
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
mesh_terms: D001804:Blood Transfusion, Autologous / Q000009:adverse effects / Q000295:instrumentation* / Q000379:methods; D006348:Cardiac Surgical Procedures; D002315:Cardiopulmonary Bypass / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004573:Electrolytes / Q000097:blood; D004617:Embolism / Q000209:etiology / Q000517:prevention & control; D004912:Erythrocytes; D005240:Feasibility Studies; D006400:Hematocrit; D006440:Hemofiltration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008055:Lipids; D008482:Mediastinum / Q000601:surgery*; D046210:Microfluidic Analytical Techniques; D010316:Particle Size; D014465:Ultrasonics
chemical_list: D004573:Electrolytes; D008055:Lipids