[Method of cyanogen bromide-fluorimetry determination of trace amount of selenomethionine in grain and blood].
Selenomethionine (SeMet) reacted with cyanogen bromide (BrCN) quantitatively forms CH3SeCN. After extracted with CHCl3, the Se of CH3SeCN is acid-digested to Se(IV). Then 2,3-diaminonaphthalene is used to determine the fluorescent Se value of 4,5-benzopiaselenol. The determination limit of this method was 3 ng/g SeMet. The accuracy of 10-500 ng Se in SeMet standard was 91.8%-97.6%. RSD was 1.9%-6.3%. Recoveries for grain and blood were 92.3%-96.7%. RSD was 2.7%-5.1%. The RSD for samples was 2.7%-9.0%. Selenocystine, selenocystiene selenite and methionine did not interfere with the determination.
Yang
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Institute of Nutrition & Food Hygiene, CAPM, Beijing 100050, China.
Zhou
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Ha
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Tian
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Gu
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Xia
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D003488:Cyanogen Bromide / Q000737:chemistry; D003553:Cystine / Q000031:analogs & derivatives; D002523:Edible Grain / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D008715:Methionine; D016566:Organoselenium Compounds; D012643:Selenium / Q000032:analysis* / Q000097:blood; D012645:Selenomethionine / Q000032:analysis / Q000097:blood*
D016566:Organoselenium Compounds; C009226:selenocystine; D003553:Cystine; D012645:Selenomethionine; D008715:Methionine; D012643:Selenium; D003488:Cyanogen Bromide