title: Rapid determination of C6-aldehydes in tomato plant emission by gas chromatography-mass spectrometry and solid-phase microextraction with on-fiber derivatization.
abstract: A simple, rapid, sensitive, and solvent-free method was developed for determination of plant-signalling compounds, the three C6-aldehydes hexanal, (Z)-3-hexenal, and (E)-2-hexenal, in tomato plant emission by gas chromatography-mass spectrometry (GC-MS) and solid-phase microextraction (SPME) with on-fiber derivatization. In this method, O-2,3,4,5,6-(pentafluorobenzyl)hydroxylamine (PFBHA) in aqueous solution was first headspace adsorbed onto a 65 microm poly(dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber at 25 degrees C for 5 min, and then the fiber with adsorbed PFBHA was used for headspace extraction of tomato plant emission at 25 degrees C for 6 min. Finally, the resulting oximes adsorbed on the fiber were desorbed and analyzed by GC-MS. Extraction conditions and method validation were studied. The proposed method had low detection limit values for the three aldehydes from 0.1 to 0.5 ng/L and good precision (RSD less than 10%). In this work, the method was applied to investigation of tomato plant defense response to Helicoverpa armigera.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.forename: Ji
authors.initials: J
authors.lastname: Qian
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002852:Chromatography, Ion Exchange; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006591:Hexobarbital / Q000032:analysis; D006898:Hydroxylamines / Q000032:analysis; D018551:Solanum lycopersicum / Q000378:metabolism*; D009036:Moths; D010091:Oximes / Q000737:chemistry; D018514:Plant Structures / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D005466:Fluorocarbons
chemical_list: D000447:Aldehydes; D006591:Hexobarbital; D006898:Hydroxylamines; D010091:Oximes; C010277:Florox Reagent; D005466:Fluorocarbons