title: Molecularly engineered p(HEMA)-based hydrogels for implant biochip biocompatibility.
abstract: The strategy of phospholipid-based biomimicry has been used to molecularly engineer poly(2-hydroxyethyl methacrylate) [p(HEMA)]-based hydrogels for improved in vitro and potential in vivo biocompatibility. Two methacrylate-based monomers, poly(ethylene glycol) (200) monomethacrylate (PEGMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC), were incorporated at varying mole fractions of 0.0-0.5 mol% PEGMA and 0-10 mol% MPC respectively, into 3 mol% tetraethyleneglycol diacrylate (TEGDA) cross-linked p(HEMA) networks. Upon hydration of these engineered hydrogels, a reduction in receding contact angle from 22+/-1.2 degrees for p(HEMA) to 8+/-2.7 degrees for p(HEMA) containing 0.5:10 mol% PEGMA:MPC was observed, reflecting the significant increase in surface hydrophilicity with increasing PEGMA and MPC content upon prolonged hydration. Hydrogels containing MPC showed a temporal increase in hydrophilicity following continuous immersion in DI water over 5 days. Hydrogels containing 0.5 mol% PEGMA and MPC in the range of 5-10 mol% displayed reduced protein adsorption when incubated with the common extracellular matrix proteins; fibronectin, collagen or laminin, producing up to 64% less protein adsorption compared to p(HEMA). Compositional optima for cell viability and proliferation established from two-factor Central Composite design analysis of human muscle fibroblasts cultured on these hydrogels suggest that those containing PEGMA between 0.3 and 0.5 mol% and MPC levels around 5-10 mol% exhibit desirable characteristics for implant material coatings-high viability (>80%) with low proliferation (<40%), confirming a lack of cytotoxicity.
authors.affiliation: Center for Bioelectronics, Biosensors and Biochips (C3B), Virginia Commonwealth University, P.O. Box 843038, 601 West Main Street, Richmond, Virginia 23284-3038, USA.
authors.forename: Sheena
authors.initials: S
authors.lastname: Abraham
authors.forename: Sean
authors.initials: S
authors.lastname: Brahim
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.forename: Anthony
authors.initials: A
authors.lastname: Guiseppi-Elie
mesh_terms: D000327:Adsorption; D040761:Biomimetic Materials / Q000032:analysis / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D020100:Hydrogels / Q000032:analysis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D011102:Polyhydroxyethyl Methacrylate / Q000032:analysis / Q000737:chemistry*; D019736:Prostheses and Implants; D040081:Protein Array Analysis; D011485:Protein Binding; D013499:Surface Properties; D017155:Wettability
chemical_list: D020099:Coated Materials, Biocompatible; D016326:Extracellular Matrix Proteins; D020100:Hydrogels; D011102:Polyhydroxyethyl Methacrylate