title: [Antitumor effect and apoptosis induction in human cancer cell lines by BRM-SJS].
abstract: OBJECTIVE
  It had been observed that BRM-SJS had antitumor effect in our clinical practice. This study was designed to investigate the antitumor activity of BRM-SJS, and mechanism of its action.
  
  
  METHODS
  In vitro antitumor experiments with MTT method, meanwhile cell morphology, flow cytometry, and agarose gel electrophoresis were performed for determining apoptosis in several tumor cell lines.
  
  
  RESULTS
  BRM-SJS had antitumor effects on human Suzhou human glioma (SHG-44), breast carcinoma (MCF-7), and human pancreas carcinoma (PANC1) in vitro, the IC50 values of BRM-SJS were 0. 299 mg/ml, 1.853 mg/ml and 9.416 mg/ml respectively. At the 2. 5 mg of BRM-SJS on SHG-44 and MCF-7, marked morphological changes, including cell shrinkage and condensation of chromosomes, were observed with electric microscope. The increase of apoptosis in SHG-44 and MCF-7 cells treated with BRM-SJS extracts 0.625 -2.5 mg for 14 -48 h was observed by Annexin-V/PI flow cytometry analysis. Agarose gel electrophoresis of DNA from SHG-44 and MCF-7 cells treated with BRM-SJS extracts 1.25 -5 mg for 24 h or 48 h showed marked DNA Ladder pattern.
  
  
  CONCLUSIONS
  Antitumor activity of BRM-SJS may be related with inducement of apoptosis of tumor cells.
authors.affiliation: Cancer Institute of Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R.China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Liu
authors.forename: Ze-Guang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Xing-Jing
authors.initials: XJ
authors.lastname: Liu
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Tian
authors.lastname: Surapol
authors.forename: Di-Ya
authors.initials: DY
authors.lastname: Na
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology / Q000648:ultrastructure; D002214:Capsules; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005910:Glioma / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000648:ultrastructure; D010946:Plants, Medicinal / Q000737:chemistry; D031363:Scutellaria / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal