title: [Expression of imprinted gene Grb10 in human oocytes and preimplantation embryos].
abstract: OBJECTIVE
  To investigate the mRNA expression of imprinted gene Grb10, a candidate gene for Silver-Russell syndrome (SRS), in human oocytes and preimplantation embryos for understanding the role of Grb10 in embryogenesis and assisted reproductive technology (ART).
  
  
  METHODS
  Nested reverse transcriptase (RT)-PCR was used to analyze Grb10 gene expression in human oocytes and preimplantation embryos.
  
  
  RESULTS
  The transcripts of Grb10 were detected in human oocytes and preimplantation embryos at all stages except for the 2-cell stage.
  
  
  CONCLUSIONS
  Grb10 gene is expressed in preimplantation embryos with close association with embryogenesis.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. swjywf@yahoo.com.cn
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Shen
authors.forename: Ling-Hong
authors.initials: LH
authors.lastname: Kong
authors.forename: Shi-Ling
authors.initials: SL
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Fu-Qi
authors.initials: FQ
authors.lastname: Xing
mesh_terms: D001755:Blastocyst / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D051383:GRB10 Adaptor Protein / Q000096:biosynthesis* / Q000235:genetics; D018392:Genomic Imprinting; D006801:Humans; D009865:Oocytes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051383:GRB10 Adaptor Protein