title: Optimized synthesis of functionalized fluorescent oligodeoxynucleotides for protein labeling.
abstract: Phthalimido-alkanol solid supports were rapidly prepared from solid supported phthalic anhydride and amino alcohol condensation induced by microwaves. These supports were used to synthesize 5'-fluorescent 3'-aminoalkyl oligodeoxynucleotides allowing a two-step deprotection necessary to avoid aminolink alkylation. After conversion into an NHS derivative using dissuccinimidyl suberate and an optimized isolation, they were conjugated with a protein.
authors.affiliation: Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM II, Université de Montpellier II, CC008, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Goussu
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
authors.forename: Hervé
authors.initials: H
authors.lastname: Bazin
authors.forename: Eric
authors.initials: E
authors.lastname: Trinquet
authors.forename: Fabrice
authors.initials: F
authors.lastname: Maurin
authors.forename: François
authors.initials: F
authors.lastname: Morvan
mesh_terms: D002232:Carbocyanines; D005456:Fluorescent Dyes; D018796:Immunoconjugates / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D009838:Oligodeoxyribonucleotides; D011506:Proteins; C085321:cyanine dye 5