[Immunopathological and histopathological aspects of ectopic implantation with possible effect on fertility disorders].
OBJECTIVE
To evaluate leucocytic infiltration of fetomaternal interface in ectopic pregnancy and to evaluate the changes in cell immunity against trophoblast (AT-CMI) in women with extrauterine pregnancy (GEU) in their medical history. To assess the effect of these factors on possible fertility disorders in a woman.


METHODS
A retrospective study.


METHODS
Mother and Child Care Institute, Prague.


METHODS
In most of the patients, we addressed GEU through laparoscopy. The tube was extirpated in toto and immediately fixed in Baker's solution. Thereafter, it was prepared in a dissection microscope and then processed in a standard way. In order to identify the intensity of AT-CMI, we used the leucocyte migration inhibition test. The cytotrophoblastic cell line JAR was used as an antigen. The degree of inhibition of the migration was monitored by means of a computer image analyser. Inhibition of migration below 75% was rated as favourable.


RESULTS
We monitored the presence of inflammatory infiltrate in the place of implantation and correlated the findings with the hCG levels and the presence of the foetal ovum or its part in the tube. In 28 patients (23.5%) of the total number of 119 patients in the group, we observed an inflammatory infiltrate in the place of implantation. In these patients, the hCG levels were lower and in 17 of them (60.7%) we did not prove the presence of a foetal ovum or its parts. In women with GEU in their medical history, the AT-CMI positivity was established in 61.1% of the women 1 year after surgery, in 56.8% of the women 1-3 years after surgery and in 41.2% of the women 3 years after surgery.


CONCLUSIONS
Ectopic pregnancy involves a pathological fetomaternal interface. The leucocytic infiltrate in the area of implantation may be of secondary character and may cause gradual destruction of the ectopically positioned product of conception. The results of our study indicate a possible participation of the increased AT-CMI in the destruction of the ectopically located trophoblast. Persisting anti-trophoblast immunity may influence the occurrence and course of further gravidities.
Kucera
E
E
Ustav pro péci o matku a díte, Praha. ed_kuc@seznam.cz
Madar
J
J
Jirásek
J E
JE
Chaloupková
A
A
D002464:Cell Migration Inhibition; D006063:Chorionic Gonadotropin / Q000097:blood; D010064:Embryo Implantation; D005187:Fallopian Tubes / Q000473:pathology*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000209:etiology*; D007249:Inflammation; D007962:Leukocytes / Q000473:pathology*; D011247:Pregnancy; D011274:Pregnancy, Tubal / Q000150:complications / Q000276:immunology* / Q000473:pathology*; D014327:Trophoblasts / Q000276:immunology*
D006063:Chorionic Gonadotropin