Surface triggering receptor expressed on myeloid cells 1 expression patterns in septic shock.
OBJECTIVE
To analyze the pattern of cell-surface expression of the triggering receptor expressed on myeloid cells (TREM) 1 during septic shock.


METHODS
Prospective clinical study in an adult 16-bed medical ICU.


METHODS
25 septic shock patients, 15 patients with shock of noninfectious origin and 7 healthy volunteers. Arterial blood was drawn within 12 h of admission and subjected to flow cytometry analysis after staining with anti-TREM-1 and anti-CD14 antibodies. Repeated sampling was performed on days 2, 3, 5, 7, and 14 in septic shock patients.


RESULTS
Monocytic TREM-1 expression was significantly higher in septic shock patients (mean fluorescence intensity 2.3+/-0.2) than in nonseptic patients (1.0+/-0.1), and healthy volunteers (1.0+/-0.1). There was no difference in monocytic TREM-1 expression between nonseptic patients and healthy volunteers or between any of the three groups with respect to TREM-1 expression on neutrophils. The time course of TREM-1 expression on monocytes diverged significantly by day 3 between survivors and ns.


CONCLUSIONS
The specificity of TREM-1 regulation by infection is highlighted. Moreover, surface TREM-1 expression on monocytes may prove useful in allowing the follow-up of septic patients during the course of the disease.
Gibot
Sébastien
S
Réanimation Médicale, Hôpital Central, 54000 Nancy, France. s.gibot@chu-nancy.fr
Le Renard
Pol-Edern
PE
Bollaert
Pierre-Edouard
PE
Kolopp-Sarda
Marie-Nathalie
MN
Béné
Marie-Christine
MC
Faure
Gilbert C
GC
Lévy
Bruno
B
D000368:Aged; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007362:Intensive Care Units; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology*; D011446:Prospective Studies; D011971:Receptors, Immunologic / Q000276:immunology*; D012772:Shock, Septic / Q000276:immunology*; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1