title: [A study on relationship between rat brain tissues hurts of the gas explosion and the expression of protein kinase CamRNA and c-fos gene.].
abstract: OBJECTIVE
  To explore relationship between rat brain tissues hurts of gas explosion and the expression of Protein Kinase C alpha mRNA.
  
  
  METHODS
  Build up rat hurt model of gas explosion. In Situ Hybridization (IDH) technique was used to test Protein Kinase C alpha mRNA. Immunohistochemical Assays (IHA) was used to determine c-fos gene protein.
  
  
  RESULTS
  Only a little a mount expression of PKC alpha mRNA and c-fos of the control group was detected. The expression of the cerebral cortex and hippocampus of PKC alpha mRNA 24 h, 48 h and the 48 h increased obviously, and the 48 h reached the peak of expression; (t = 4.12 P < 0.01). The expression of c-fos protein of the cerebral cortex and hippocampus started to increase obviously at 0.5 h and the 4 h reached the peak, then the strength lowered gradually and the expression level came back normal level on fifth day.
  
  
  CONCLUSIONS
  The anoxia of brain tissues due to the gas explosion may promote the expression of PKCamRNA, and PKCamRNA could regulate the expression of the gene of c-fos. Both PKCamRNA and the gene of c-fos are involved in harmful processes to the nerve cells.
authors.affiliation: Hospital of Pingdingshan coal industry group, Henan 467000, China.
authors.forename: Gui-Zhi
authors.initials: GZ
authors.lastname: Wang
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Ren
authors.forename: Zhu-Xuan
authors.initials: ZX
authors.lastname: Qin
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
authors.forename: Song-Yuan
authors.initials: SY
authors.lastname: Wu
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin-Hong
authors.initials: LH
authors.lastname: Liu
authors.forename: Liang-Ming
authors.initials: LM
authors.lastname: Liu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D004195:Disease Models, Animal; D005107:Explosions; D000860:Hypoxia / Q000378:metabolism; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013665:Tear Gases
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D013665:Tear Gases; D051571:Protein Kinase C-alpha