title: Attenuation of lipid peroxidation and hyperlipidemia by quercetin glucoside in the aorta of high cholesterol-fed rabbit.
abstract: Antioxidative activity of dietary flavonoids is suggested to be, at least partly, responsible for a wide variety of their biological effects relating to anti-atherosclerosis. However, it is not known whether dietary flavonoids reach to the target site and act as antioxidants. In this study, we tried to evaluate the antioxidative effect of quercetin 3-O-beta-D-glucoside (Q3G), a typical flavonoid present in vegetables, in rabbit aorta. New Zealand White rabbits were fed a control diet (control group), 2.0% cholesterol diet (HC group) and 2.0% cholesterol plus 0.1% Q3G (HC + Q3G group) for one month. The amounts of total cholesterol, triacylglycerol and total fatty acids in both the plasma and aorta were significantly lower in the HC + Q3G group as compared with the HC group. Quercetin was detected in the aorta of the HC + Q3G group after enzymatic deconjugation, indicating that quercetin accumulated as conjugated metabolites in the aorta. The contents of TBA-reacting substances (TBARS) and cholesteryl ester hydroperoxides (CEOOH) in the aorta of the HC + Q3G group were significantly lower than those in the HC group. The aorta of HC + Q3G group was more resistant than that of HC group in copper ion-induced lipid peroxidation ex vivo. HC + Q3G group accumulated a higher amount of vitamin E per total cholesterol than HC group in the aorta. These results strongly suggest that quercetin glucosides accumulate in the aorta as their metabolites and attenuate lipid peroxidation occurring in the aorta, along with the attenuation of hyperlipidemia.
authors.affiliation: Department of Food Science, Graduate School of Nutrition and Biosciences, The University of Tokushima, Kuramoto-cho, 3-18-15 Tokushima, 770-8503 Japan.
authors.forename: Chiemi
authors.initials: C
authors.lastname: Kamada
authors.forename: Edson L
authors.initials: EL
authors.lastname: da Silva
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ohnishi-Kameyama
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Moon
authors.forename: Junji
authors.initials: J
authors.lastname: Terao
mesh_terms: D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000187:drug effects*; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D003300:Copper / Q000494:pharmacology; D004435:Eating; D005227:Fatty Acids / Q000097:blood; D006128:Growth; D006949:Hyperlipidemias / Q000139:chemically induced / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D024502:alpha-Tocopherol / Q000378:metabolism
chemical_list: D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D005227:Fatty Acids; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; C081459:cholesteryl ester hydroperoxide; D003300:Copper; D002784:Cholesterol; D011794:Quercetin; D024502:alpha-Tocopherol