title: Stem cell factor has a suppressive activity to IgE-mediated chemotaxis of mast cells.
abstract: Stem cell factor (SCF), which is well known as a cytokine capable of amplifying development and functions of mast cells, is mainly released from fibroblasts in the peripheral tissue. To investigate whether SCF controlled chemotactic migration of mast cells induced by IgE-specific Ag, murine bone marrow-derived cultured mast cells (BMCMC) and human cord blood-derived cultured mast cells (HuCMC) were preincubated with SCF. Although BMCMC and HuCMC sensitized with IgE directly moved toward specific Ag, preincubation for even 1 h with an optimal dose of SCF suppressed the IgE-mediated chemotactic movement. No or little inhibitory effect of SCF was detected in BMCMC derived from c-kit receptor-defect WBB6F1-W/Wv mice. In contrast, preincubation of BMCMC and HuCMC with SCF enhanced beta-hexosaminidase release and Ca2+ mobilization in response to Ag after sensitization with IgE. Using the real-time record of chemotactic migration, BMCMC preincubated with SCF manifested motionless without degranulation. These results suggest that locally produced SCF may have an inhibitory effect on chemotaxis of mast cells, contributing to their accumulation and enhancement of functions at the peripheral site in allergic and nonallergic conditions.
authors.affiliation: Laboratory of Molecular Pathology and Therapeutics, Division of Animal Life Science, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Sawada
authors.forename: Shinya
authors.initials: S
authors.lastname: Shimizu
authors.forename: Takuya
authors.initials: T
authors.lastname: Tamatani
authors.forename: Shiro
authors.initials: S
authors.lastname: Kanegasaki
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Saito
authors.forename: Akane
authors.initials: A
authors.lastname: Tanaka
authors.forename: Naotomo
authors.initials: N
authors.lastname: Kambe
authors.forename: Tatsutoshi
authors.initials: T
authors.lastname: Nakahata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D020013:Calcium Signaling / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects*; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D007231:Infant, Newborn; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D020932:Nerve Growth Factor / Q000494:pharmacology; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D017455:Receptors, IgE / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis
chemical_list: D000941:Antigens; D004136:Dinitrobenzenes; D007166:Immunosuppressive Agents; D017455:Receptors, IgE; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D007073:Immunoglobulin E; D020932:Nerve Growth Factor; D019009:Proto-Oncogene Proteins c-kit; D001619:beta-N-Acetylhexosaminidases