Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway.
The substrates for Bacillus subtilis PLP synthase (YaaD and YaaE) are identified, and the first reconstitution of PLP biosynthesis using this pathway is described. Three partial reactions catalyzed by YaaD are also identified.
Burns
Kristin E
KE
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Xiang
Yun
Y
Kinsland
Cynthia L
CL
McLafferty
Fred W
FW
Begley
Tadhg P
TP
D000878:Anthranilate Synthase / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D005583:Fourier Analysis; D005972:Glutaminase / Q000378:metabolism; D019884:Nitrogenous Group Transferases / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000096:biosynthesis*; D021241:Spectrometry, Mass, Electrospray Ionization
D001426:Bacterial Proteins; D011732:Pyridoxal Phosphate; D019884:Nitrogenous Group Transferases; D005972:Glutaminase; D000878:Anthranilate Synthase; C020655:anthranilate synthase, glutamine amidotransferase subunit