[Molecular reasons for the regulation of the enzymatic activity of bovine pancreatic deoxyribonuclease I as studied by dynamic light scattering and fluorescent spectroscopy].
The effect of catalytic bivalent and inhibitory monovalent cations on the interactions of bovine pancreatic deoxyribonuclease I with the circle single-stranded DNA of M13 phase was studied. It was found that monovalent cations affect the site of binding to DNA and the active center of the enzyme; in their presence, a break of the formation of the enzyme-substrate complex occurs. The results provide evidence that conformational changes of the protein molecule in all cases are more substantial that it could be expected from X-ray data published earlier.
Danilova
I G
IG
Shevelev
I V
IV
Isaev-Ivanov
V V
VV
Noskin
L A
LA
D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics; D002417:Cattle; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D007834:Lasers; D008027:Light; D010179:Pancreas / Q000201:enzymology*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003850:Deoxyribonuclease I