title: In situ control of cell adhesion using photoresponsive culture surface.
abstract: A photoresponsive culture surface (PRCS) allowing photocontrol of cell adhesion was prepared with a novel polymer material composed of poly(N-isopropylacrylamide) having spiropyran chromophores as side chains. Cell adhesion of the surface was drastically enhanced by the irradiation with ultraviolet (UV) light (wavelength: 365 nm); after subsequent cooling and washing on ice, many cells remained in the irradiated region, whereas most cells were removed from the nonirradiated region. The cell adhesion of the PRCS, which had been enhanced by previous UV irradiation, was reset by the visible light irradiation (wavelength 400-440 nm) and the annealing at 37 degrees C for 2 h. Also it was confirmed that the regional control of cell adhesion was induced several times by repeating the same series of operations. Further, living cell patterning with the 200 microm line width was produced readily by projecting UV light along a micropattern on the PRCS on which the living cells had been seeded uniformly in advance. By using a fluorescent probe that stains living cells only, it was confirmed that the cells maintained sufficient viability even after UV light irradiation followed by cooling and washing.
authors.affiliation: Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Edahiro
authors.forename: Kimio
authors.initials: K
authors.lastname: Sumaru
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tada
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Ohi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takagi
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Kameda
authors.forename: Toshio
authors.initials: T
authors.lastname: Shinbo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshimi
mesh_terms: D000180:Acrylic Resins / Q000528:radiation effects; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D010777:Photochemistry / Q000379:methods*; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D000180:Acrylic Resins; C052970:poly-N-isopropylacrylamide