title: Conformational substates of calmodulin revealed by single-pair fluorescence resonance energy transfer: influence of solution conditions and oxidative modification.
abstract: A calmodulin (CaM) mutant (T34,110C-CaM) doubly labeled with fluorescence probes AlexaFluor 488 and Texas Red in opposing domains (CaM-DA) has been used to examine conformational heterogeneity in CaM by single-pair fluorescence resonance energy transfer (spFRET). Burst-integrated FRET efficiencies of freely diffusing CaM-DA single molecules yielded distributions of distance between domains of CaM-DA. We recently reported distinct conformational substates of Ca(2+)-CaM-DA and apoCaM-DA, with peaks in the distance distributions centered at approximately 28 A, 34-38 A, and 55 A [Slaughter et al. (2004) J. Phys. Chem. B 108, 10388-10397]. In the present study, shifts in the amplitudes and center distances of the conformational substates were detected with variation in solution conditions. The amplitude of an extended conformation was observed to change as a function of Ca(2+) over a free Ca(2+) range that is consistent with binding to the high affinity, C-terminal Ca(2+) binding sites, suggesting the existence of communication between lobes of CaM. Lowering pH shifted the relative amplitudes of the conformations, with a marked increase in the presence of the compact conformations and an almost complete absence of the extended conformation. In addition, the single-molecule distance distribution of apoCaM-DA at reduced ionic strength was shifted to longer distance and showed evidence of an increase in conformational heterogeneity relative to apoCaM-DA at physiological ionic strength. Oxidation of methionine residues in CaM-DA produced a substantial increase in the amplitude of the extended conformation relative to the more compact conformation. The results are considered in light of a hypothesis that suggests that electrostatic interactions between charged amino acid side chains play an important role in determining the most stable CaM conformation under varying solution conditions.
authors.affiliation: Department of Chemistry, 1251 Wescoe Hall Drive, University of Kansas, Lawrence, Kansas 66045-7582, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Slaughter
authors.forename: Jay R
authors.initials: JR
authors.lastname: Unruh
authors.forename: Michael W
authors.initials: MW
authors.lastname: Allen
authors.forename: Ramona J
authors.initials: RJ
authors.lastname: Bieber Urbauer
authors.forename: Carey K
authors.initials: CK
authors.lastname: Johnson
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D002147:Calmodulin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008956:Models, Chemical; D010084:Oxidation-Reduction; D011487:Protein Conformation; D017434:Protein Structure, Tertiary / Q000235:genetics; D012996:Solutions; D055672:Static Electricity; D013388:Succinimides / Q000378:metabolism; D013816:Thermodynamics; D014966:Xanthenes / Q000378:metabolism
chemical_list: C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; D002147:Calmodulin; D005456:Fluorescent Dyes; D012996:Solutions; D013388:Succinimides; D014966:Xanthenes; C034657:Texas red; D008715:Methionine