[Evaluation study on determination of ZPP in blood with hematofluorometer].
The authors studied the normalization and evaluation of the method detecting the zinc protoporphyrin (ZPP) by direct displaying blood fluorescent. The results revealed that this method was highly accurate and precise. The relative humidity not more than 90% had no influence to the results. The detection limit of this method was 0.1 microg/g. The linear range of standard curve was 0-15 microg/g, and the precision in batch RSD=0.27%-1.93%. On the accuracy side, the three concentration curving values of ZPP standard material in blood were all within the range of indefinition of given values. If the samples were not detected rapidly and the blood was diluted by heparin, it might be preserved at 4 degrees C for 3 days, at -8 degrees C for 8 days. The blank fluorescent value of glassy cover made in China is less than 3.5 microg/g, Hb can substitute KLI glassy cover. This method can analyse one sample per minute, has advantage of rapidity, convenience, accuracy and reliablity.
He
B
B
Qingdao Public Health and Anti-epidemic Station, Qingdao 266001, China.
Wang
S
S
Hao
W
W
D002681:China; D004396:Coloring Agents; D005470:Fluorometry / Q000379:methods*; D057230:Limit of Detection; D010710:Phosphates; D011524:Protoporphyrins / Q000097:blood*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D004396:Coloring Agents; D010710:Phosphates; D011524:Protoporphyrins; C017803:zinc protoporphyrin; C009285:sodium polymetaphosphate