title: Selective and validated spectrophotometric methods for the determination of nicorandil in pharmaceutical formulations.
abstract: Two simple and sensitive validated spectrophotometric methods have been described for the assay of nicorandil in drug formulations. Method A is based on the reaction of the drug with phloroglucinol-sulfanilic acid reagent in sulfuric acid medium to give yellow-colored product, which absorbs maximally at 425 nm. Method B uses the oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) with DL- 3,4 - dihydroxyphenylalanine (DL-dopa) in the presence of nicorandil as oxidant in sulfuric acid medium to form an intensely colored product having maximum absorbance at 530 nm. Beer's law is obeyed in the concentration range 2.5 to 50.0 and 1.0 to 15.0 microg mL(-1) with methods A and B, respectively. Both methods have been successfully applied for the analysis of drug in pharmaceutical formulations. The reliability and the performance of the proposed methods are established by point and interval hypothesis and through recovery studies. The experimental true bias of all samples is smaller than +/-2%.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, India. cht17nr@yahoo.co.in
authors.forename: Nafisur
authors.initials: N
authors.lastname: Rahman
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Ahmad
authors.forename: Syed Najmul Hejaz
authors.initials: SN
authors.lastname: Azmi
mesh_terms: D052160:Benzothiazoles; D002138:Calibration; D004295:Dihydroxyphenylalanine / Q000737:chemistry; D006358:Hot Temperature; D006835:Hydrazones; D007202:Indicators and Reagents; D020108:Nicorandil / Q000032:analysis*; D010696:Phloroglucinol / Q000737:chemistry; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods* / Q000592:standards; D013425:Sulfanilic Acids / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D013997:Time Factors; D014665:Vasodilator Agents / Q000032:analysis*
chemical_list: D052160:Benzothiazoles; D006835:Hydrazones; D007202:Indicators and Reagents; D013425:Sulfanilic Acids; D013464:Sulfuric Acids; D013844:Thiazoles; D014665:Vasodilator Agents; D020108:Nicorandil; D004295:Dihydroxyphenylalanine; D010696:Phloroglucinol; C033158:sulfuric acid; C002536:3-methyl-2-benzothiazolone hydrazone