Fluorescence in situ hybridization and spectral imaging analysis of human oocytes and first polar bodies.
We investigated the frequencies of abnormalities involving either chromosome 1, 16, 18, or 21 in failed-fertilized human oocytes. Although abnormalities involving chromosome 16 showed an age-dependent increase, results for the other chromosomes did not show statistically significant differences among the three age groups, <35 years, 35-39 years, and >39 years. The scoring of four chromosomes is likely to underestimate the true rate of aneuploid cells. Therefore, for a pilot study investigating a more-comprehensive analysis of oocytes and their corresponding first polar bodies, we developed a novel eight-probe chromosome enumeration scheme using fluorescence in situ hybridization and spectral imaging analysis.
Weier
Heinz-Ulli G
HU
Life Sciences Division, MS 74-157, E.O. Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA. ulliweier@hotmail.com
Weier
Jingly F
JF
Renom
Maria Oter
MO
Zheng
Xuezhong
X
Colls
Pere
P
Nureddin
Aida
A
Pham
Chau D
CD
Chu
Lisa W
LW
Racowsky
Catherine
C
Munné
Santiago
S
D000328:Adult; D000367:Age Factors; D000782:Aneuploidy; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D009865:Oocytes / Q000166:cytology* / Q000648:ultrastructure