title: Isolation and expression analysis of a Pax group III gene from the crustacean Cherax destructor.
abstract: Pax genes encode transcription factors that are critical regulators of key developmental processes in evolutionarily diverse animal phyla. Here we report the first isolation of a Pax gene from a crustacean: a Pax group III gene we have termed CdpaxIII that contains highly conserved DNA-binding domains, the paired domain and homeodomain. CdpaxIII is expressed in the embryo, in adult limb muscle during both quiescence and regeneration, and during the distinct process of epimorphic limb regeneration. Interestingly, CdpaxIII is expressed as two distinct alternate transcripts, one of which is novel in lacking a large portion of its paired domain.
authors.affiliation: School of Natural Sciences, Centre for Ecosystem Management, Edith Cowan University, 100 Joondalup Drive, Joondalup, WA, 6027, Australia. rwhite@ecu.edu.au
authors.forename: Robert B
authors.initials: RB
authors.lastname: White
authors.forename: Tina M
authors.initials: TM
authors.lastname: Lamey
authors.forename: Mel
authors.initials: M
authors.lastname: Ziman
authors.forename: Annette
authors.initials: A
authors.lastname: Koenders
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003445:Crustacea / Q000235:genetics*; D005121:Extremities / Q000502:physiology; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D051761:Paired Box Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D051761:Paired Box Transcription Factors; D012333:RNA, Messenger