Induction of immune responses and partial protection in mice after skin immunization with rotavirus VP6 protein and the adjuvant LT(R192G).
Oral or intranasal administration of mice with rotavirus VP6/LT(R192G) vaccine induces between 95 and 99% protection against fecal shedding of rotavirus after challenge. However, mucosal administration of LT(R192G) is controversial. Subcutaneous, intradermal or Biojector injection induced high titers of serum VP6-specific IgG, eliciting only partial to no protection (73, 0 and 26%, respectively), while transcutaneous delivery using gauze pad induced both poor immune responses and no protection (13%). A mixture of VP6-derived synthetic peptides induced >97, 48 and 33% protection after intranasal, gauze pad or Biojector administration, respectively. For needle-free delivery methods to be viable, improvements to these methods must be made to enhance the efficacy of the VP6 vaccine.
Choi
Anthony H-C
AH
Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229, USA. anthony.choi@cchmc.org
Smiley
Kristi
K
Basu
Mitali
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000279:Administration, Cutaneous; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D001427:Bacterial Toxins / Q000008:administration & dosage*; D036022:Capsid Proteins / Q000276:immunology*; D004768:Enterotoxins / Q000008:administration & dosage*; D029968:Escherichia coli Proteins / Q000008:administration & dosage*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007277:Injections, Jet; D051379:Mice; D008807:Mice, Inbred BALB C; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology* / Q000517:prevention & control*; D014765:Viral Vaccines / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D001427:Bacterial Toxins; D036022:Capsid Proteins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; C061332:VP6 protein, Rotavirus; D014765:Viral Vaccines; C027538:heat-labile enterotoxin, E coli