Quantitative determination of vigabatrin and gabapentin in human serum by gas chromatography-mass spectrometry.
BACKGROUND
Published methods for routine clinical monitoring of vigabatrin and gabapentin are often very laborious. A simple GC-MS method was developed for the simultaneous quantitative determination of vigabatrin and gabapentin in human serum.


METHODS
After protein precipitation, the compounds are derivatized by methylation and analysed on a polydimethylsiloxane column using splitless injection. Cyclobarbital is used as the internal standard. To attain maximal sensitivity, detection is performed in selected ion monitoring mode.


RESULTS
The method was fully validated and linear calibration curves were obtained in the concentration ranges from 5 to 80 microg/mL for vigabatrin and from 5 to 30 microg/mL for gabapentin. The within-day and day-to-day relative standard deviations at three different concentration levels were <10% and <15%, respectively. The limit of quantitation was 2 mug/mL for both compounds.


CONCLUSIONS
The presented method provides high chromatographic resolution, good sensitivity and unequivocal identification potential and can be used for simultaneous analysis of both antiepileptics.
Borrey
Daniëlle C R
DC
Toxicology Unit, Department of Clinical Chemistry, AZ Sint-Jan AV, Ruddershove 10, B-8000 Brugge, Belgium. danielle.borrey@azbrugge.be
Godderis
Kristof O
KO
Engelrelst
Veronique I L
VI
Bernard
Dirk R
DR
Langlois
Michel R
MR
D000588:Amines / Q000097:blood*; D002138:Calibration; D003509:Cyclohexanecarboxylic Acids / Q000097:blood*; D000077206:Gabapentin; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020888:Vigabatrin / Q000097:blood*; D005680:gamma-Aminobutyric Acid / Q000097:blood*
D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D020888:Vigabatrin