title: [Binding and inhibition of adeno-associated virus Rep78 protein with hepatitis B virus C promoter].
abstract: OBJECTIVE
  Adeno-associated virus (AAV) Rep78 is known for its inhibitory effects on replication of several viruses and oncogenes transformations. The study was to investigate the effect of Rep78 on hepatitis B virus C (HBV-C) gene and the mechanism of it.
  
  
  METHODS
  HBV-C promoter and HBV-C gene with its promoter were amplified by PCR and labeled with 32P-ATP. Electrophoretic mobility shift assay (EMSA) and in vitro transcription were utilized to detect the binding of MBP-Rep78 with HBV-C promoter and the transcription of HBV-C gene.
  
  
  RESULTS
  EMSA showed that by increasing the amount of Rep78 protein from 0.1 microg to 1.0 microg, the binding bands got stronger in a dose-dependent manner. In addition, Rep78 antibody was used to certify the specificity of this binding. The compound of Rep78, Rep78 antibody and HBV-C promoter were seen as super shift bands in EMSA. Meanwhile, HBV-C gene transcription was significantly inhibited by in vitro transcription which meant that Rep78 could not only bind with HBV-C promoter, but also could inhibit the transcription of HBV-C gene.
  
  
  CONCLUSIONS
  AAV Rep78 could inhibit the transcription of HBV-C gene through its binding with HBV-C promoter.
authors.affiliation: Liver Disease Research Center, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.
authors.forename: Zhong-yu
authors.initials: ZY
authors.lastname: Yan
authors.forename: Min
authors.initials: M
authors.lastname: Cong
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Shu-zhen
authors.initials: SZ
authors.lastname: Tang
authors.forename: Bao-en
authors.initials: BE
authors.lastname: Wang
authors.forename: Ji-dong
authors.initials: JD
authors.lastname: Jia
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Yong
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics*; D000229:Dependovirus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins; C068734:rep proteins, Adeno-associated virus 2