Rubredoxin acts as an electron donor for neelaredoxin in Archaeoglobus fulgidus.
Archaeoglobus fulgidus neelaredoxin (Nlr) is an electron donor:superoxide oxidoreductase. The reaction of superoxide with reduced Nlr is almost diffusion-limited, but the overall efficiency for detoxifying superoxide in vivo depends on the rate of reduction of Nlr by electron donors. Here, we report the purification and characterization of the two type I rubredoxins from A. fulgidus (AF0880 and AF1349) and show that they act as efficient electron donors for neelaredoxin, in vitro, with a second-order rate constant of 10(7)M(-1)s(-1) at 10 degrees C and pH 7.2.
Rodrigues
João V
JV
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República (EAN) Apt 127, 2781-901 Oeiras, Portugal.
Abreu
Isabel A
IA
Saraiva
Lígia M
LM
Teixeira
Miguel
M
D000595:Amino Acid Sequence; D019611:Archaeoglobus fulgidus / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins; D004579:Electron Transport; D033862:Iron-Binding Proteins / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D012416:Rubredoxins / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D013482:Superoxide Dismutase; D017186:Titrimetry
D001426:Bacterial Proteins; D033862:Iron-Binding Proteins; D012416:Rubredoxins; D010088:Oxidoreductases; D013482:Superoxide Dismutase; C414179:nlr protein, Desulfovibrio gigas; C023091:rubredoxin-NAD+ reductase; D009247:NADH, NADPH Oxidoreductases