[Determination of deoxyribonucleic acid with nitro-tetrazolium chloride blue by resonance light-scattering technique].
Characteristics of the interaction of nitro-tetrazolium chloride blue and Deoxyribonucleic Acid (DNA) were studied in the of pH 1.8 buffer solution by Resonance Light-Scattering (RLS) technique. The effects of interference substances and the optimization of the procedure were investigated, and a new method for the determination of the DNA was established based on RLS. The linear range of the DNA is 0-7 mg x L(-1) with a correlation coefficient of 0.9958. The detection limit is 30.04 microg x L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.
Long
Yun-fei
YF
Institute of Chemistry, Xiangtan University, Xiangtan 411105, China.
Chen
Xiao-ming
XM
Cai
Tie-jun
TJ
D002712:Chlorides / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008027:Light; D057230:Limit of Detection; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts / Q000737:chemistry*
D002712:Chlorides; D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue; D004247:DNA