[Anti-measles DNA-immunization in experiment: immunogenicity and safety].
The plasmid DNA pCDNA3.1-H encoding the N-terminal sequence of the measles hemagglutinin (H) protein was constructed. Virus-specific particles (VSP) containing the plasmid DNA pCDNA3.1-H coated by the spermidine-polyglucin complex. The mice were immunized by VSP. ELISA, HAIT and immunoblot showed a shaping specific humoral response. The sera of immunized animals were proven to neutralize the wild strain of the NOV96 measles virus. The formation of the specific cell immunity was confirmed by erythrocyte proliferation assay and ELISpot. PCR was used to detect the presence of the plasmid DNA in different intestines and tissues of animals after a single immunization. It was not detected at any time interval in the brain, liver, thymus and blood. And it was present on days 7 and 14 in the red bone marrow, spleen, muscular tissue, lungs and fatty tissue. On day 21 the plasmid DNA was not detected in any of the investigated organs.
Maksimov
N L
NL
Bukin
E K
EK
Agafonov
A P
AP
Neverov
A A
AA
Karpenko
L I
LI
Il'ichev
A A
AA
Ignat'ev
G M
GM
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001853:Bone Marrow / Q000378:metabolism; D002455:Cell Division; D018076:DNA, Complementary / Q000032:analysis; D004912:Erythrocytes / Q000166:cytology; D006389:Hemagglutinins, Viral / Q000235:genetics; D007273:Injections, Intramuscular; D008168:Lung / Q000378:metabolism; D008457:Measles / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D018103:Morbillivirus / Q000235:genetics / Q000276:immunology*; D018482:Muscle, Skeletal / Q000378:metabolism; D010957:Plasmids / Q000008:administration & dosage; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology
D000914:Antibodies, Viral; D018076:DNA, Complementary; D006389:Hemagglutinins, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines