title: [Expression of Interleukin-8 in KB cells infected with Candida albicans].
abstract: OBJECTIVE
  To investigate the change of interleukin-8 (IL-8) in oral epithelial cells (KB cells) infected with Candida albicans.
  
  
  METHODS
  Two-site ELISA and RT-PCR were applied to determine the levels of IL-8 protein or mRNA expression in the KB cells induced by hyphae, yeast, killed Candida albicans and medium respectively. The unpaired Student's t test was used to analyse the data.
  
  
  RESULTS
  Candida albicans induced the KB cells secrete more IL-8. The hyphae, yeast, killed Candida albicans and control group marks of RT-PCR were 180.23, 186.36, 143.41 and 85.57 respectively, calculated using a two-tailed test, P<0.05, was considered to be significant, but the medium group (mark 80.87) had no significant change. The tendency of the IL-8 protein secreted by KB cells increased with the time but reduced when Candida albicans was more.
  
  
  CONCLUSIONS
  Candida albicans is responsible for the production of IL-8 secreted by oral epithelial cells which can be expected to play an important role in the infection and defection with the local oral mucosa.
authors.affiliation: College of Stomatology, China Medical University. Shenyang 110002,Liaoning Province, China. zhanglingab@yahoo.com
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhao-Yuan
authors.initials: ZY
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Sun
mesh_terms: D002176:Candida albicans / Q000472:pathogenicity*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D007624:KB Cells
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8