title: General strategies for efficient adjuvant incorporation of recombinant subunit immunogens.
abstract: We have previously reported strategies for Escherichia coli production of recombinant immunogens fused to hydrophobic peptides or lipid tags to improve their capacity to be incorporated into an adjuvant formulation, e.g., immunostimulating complexes (iscoms). Recently, we also explored the strong interaction between biotin and streptavidin to achieve iscom association of recombinant immunogens. Plasmodium falciparum,Toxoplasma gondii and Neospora caninum antigens have served as model immunogens in the different studies. Generated fusion proteins have been found to be successfully incorporated into iscoms and high-titer antigen-specific antibody responses have been obtained upon immunization of mice. We believe that the different concepts presented, utilizing either hydrophobic peptide or lipid tags, or the recently explored biotin-streptavidin principle, offer convenient methods to achieve efficient adjuvant incorporation of recombinant immunogens.
authors.affiliation: Department of Biotechnology, AlbaNova University Center, Kungl Tekniska Högskolan (KTH), SE-10691 Stockholm, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Wikman
authors.forename: Mikaela
authors.initials: M
authors.lastname: Friedman
authors.forename: Sunan
authors.initials: S
authors.lastname: Pinitkiatisakul
authors.forename: Christin
authors.initials: C
authors.lastname: Andersson
authors.forename: Andrew
authors.initials: A
authors.lastname: Hemphill
authors.forename: Karin
authors.initials: K
authors.lastname: Lövgren-Bengtsson
authors.forename: Anna
authors.initials: A
authors.lastname: Lundén
authors.forename: Stefan
authors.initials: S
authors.lastname: Ståhl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D001710:Biotin; D004926:Escherichia coli / Q000235:genetics; D016882:ISCOMs; D007114:Immunization; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D019809:Streptavidin; D022223:Vaccines, Subunit / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics
chemical_list: D000941:Antigens; D016882:ISCOMs; D007155:Immunologic Factors; D008055:Lipids; D011993:Recombinant Fusion Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D001710:Biotin; D019809:Streptavidin