Comparison of classical and derivative UV-spectrophotometric methods for the quantification of diltiazem and mexiletine.
A first order derivative UV-spectrophotometric method for the determination of diltiazem hydrochloride and mexiletine hydrochloride has been developed and validated. In the assay, the first- and second-order measurements with the use of the "peak-zero" and "peak-peak" techniques were applied. The linear correlation (r < 0.9999) between the amplitude of the peak and the concentration of the examined drugs in the range of 3.0-8.0 microg mL(-1) for diltiazem and 50-100 microg mL(-1) for mexiletine was obtained. The proposed method was successfully applied for accurate (mean recovery about 100%), precise (RSD about 1%) and selective determination of the studied drug in the pure and dosage forms.
Pietraś
Rafal
R
Department of Medicinal Chemistry, Medical University of Lublin, 6 Chodźki Str., 20-093 Lublin, Poland.
Kowalczuk
Dorota
D
Hopkała
Hanna
H
D000889:Anti-Arrhythmia Agents / Q000032:analysis* / Q000737:chemistry; D002214:Capsules; D004110:Diltiazem / Q000032:analysis* / Q000737:chemistry; D008801:Mexiletine / Q000032:analysis* / Q000737:chemistry; D019999:Pharmaceutical Solutions; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013607:Tablets
D000889:Anti-Arrhythmia Agents; D002214:Capsules; D019999:Pharmaceutical Solutions; D013607:Tablets; D008801:Mexiletine; D004110:Diltiazem