Antibodies generated by a novel DNA vaccination identify the MHC class III encoded BAT2 polypeptide.
Recombinant vaccines containing Ii sequences were employed to elicit an antibody response. Gene gun immunisation of mice with the recombinant Ii-antigen-encoding vectors induced antigen-specific antibodies. Antibody levels were substantially elevated when the DNA construct was extended by a sequence encoding the protease inhibitory domain of the invariant chain isoform Ii41. Employing this approach, we raised antibodies specific for a novel MHC class III encoded protein. The antibodies identify the 216 kDa BAT2 polypeptide. Immunostaining of embryonic tissue sections showed specific expression, especially in central nervous tissue, and suggests BAT2 as a novel differentiation marker.
Schneiders
Angelika
A
Division of Immunobiology, Institute of Molecular Physiology, University of Bonn, Römerstrasse 164, 53117 Bonn, Germany.
Thiel
Stefan
S
Winkler
Johannes
J
Möller
Peter
P
Koch
Norbert
N
D000818:Animals; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics* / Q000276:immunology; D002490:Central Nervous System / Q000737:chemistry; D005333:Fetus / Q000737:chemistry; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D051379:Mice; D023421:Models, Animal; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D011506:Proteins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C045084:invariant chain; C092008:PRRC2A protein, human