title: [Principle of determining the composition of complexes with the ratio of double peak values at double wavelengths by UV spectrophotometry].
abstract: In this paper, a new method for determining composition ratio of complexes was studied with ratio of double peak values at double wavelengths by UV spectrophotometry. The ratio of the maximum peak value to the minimum peak value and the composition ratio of complexes are the same. The calculation formula for the composition of complexes is deduced in accordance with beer's law and the theory of dissociation equilibrium of complexes: N = (deltaA2epsilonlambda1(MR) - deltaA1epsilonlambda2(MR)/(deltaA2epsilonlambda1(R) - deltaA1epsilonlambda2(R). The calculated value and that obtained by traditional method are agreeable. The method is reliable, simple, visual and quick.
authors.affiliation: Department of Light and Chemistry Engineering, Guangzhou University, Guangzhou 510091, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Zhang
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Zhang
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Huang
authors.forename: Wen-Jin
authors.initials: WJ
authors.lastname: Huang
mesh_terms: D000465:Algorithms; D006690:Bisbenzimidazole / Q000737:chemistry; D004563:Electrochemistry; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013057:Spectrum Analysis; D014466:Ultraviolet Rays
chemical_list: D006690:Bisbenzimidazole