Dihydropteroate synthase and novel dihydrofolate reductase gene mutations in strains of Pneumocystis jirovecii from South Africa.
Dihydropteroate synthase (DHPS) gene mutations have raised concerns about emerging sulfonamide resistance in Pneumocystis jirovecii. DHPS and dihydrofolate reductase (DHFR) gene products were amplified in clinical specimens from South African patients. One of 53 DHPS genes sequenced contained the double mutation Thr55Ala Pro57Ser. DHFR gene mutations detected were Ala67Val and the new mutations Arg59Gly and C278T.
Robberts
F J L
FJ
Department of Medical Microbiology, University of Stellenbosch, Tygerberg 7500, South Africa. lourensr@sun.ac.za
Chalkley
L J
LJ
Weyer
K
K
Goussard
P
P
Liebowitz
L D
LD
D004094:Dihydropteroate Synthase / Q000235:genetics*; D006801:Humans; D009154:Mutation; D011010:Pneumocystis / Q000201:enzymology* / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase; D004094:Dihydropteroate Synthase