Identification and characterization of ovalbumin gene Y in hen egg white.
Ovalbumin gene Y has been known as a member of the ovalbumin gene family since 1982, when its encoding gene was sequenced. In the present study, ovalbumin gene Y has been demonstrated as a new minor protein of hen egg white. This protein has been isolated by isoelectrofocalization and two-dimensional polyacrylamide gel electrophoresis and has been characterized using peptide mass fingerprinting. The concentration ratio of ovalbumin gene Y:ovalbumin is about 13:100. Unlike ovalbumin, ovalbumin gene Y is not phosphorylated, but like ovalbumin, this protein is glycosylated. Ovalbumin gene Y exists as a mixture of three molecular species, which differ in their isoelectric points. The polymorphism of this protein cannot be explained by various glycosylation levels.
Nau
Françoise
F
UMR INRA-Agrocampus Rennes Science et Technologie du Lait et de l'OEuf, 65 rue de Saint-Brieuc CS 84215, 35042 Rennes Cedex, France. Francoise.Nau@agrocampus-rennes.fr
Pasco
Maryvonne
M
Désert
Colette
C
Mollé
Daniel
D
Croguennec
Thomas
T
Guérin-Dubiard
Catherine
C
D000818:Animals; D002645:Chickens; D004527:Egg Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004529:Egg White / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D006031:Glycosylation; D007525:Isoelectric Focusing; D010047:Ovalbumin / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004527:Egg Proteins; D010446:Peptide Fragments; D010047:Ovalbumin