title: Structural characterization of the antigenic capsular polysaccharide and lipopolysaccharide O-chain produced by Actinobacillus pleuropneumoniae serotype 15.
abstract: The specific capsular polysaccharide produced by Actinobacillus pleuropneumoniae serotype 15 was determined to be a high-molecular-mass polymer having [alpha]D + 69 degrees (water) and composed of a linear backbone of phosphate diester linked disaccharide units of 2-acetamido-2-deoxy-D-glucose (D-GlcNAc) and 2-acetamido-2-deoxy-D-galactose (D-GalNAc) residues (1:1). Thirty percent of the D-GalNAc residues were substituted at O-4 by beta-D-galactopyranose (beta-D-Galp) residues. Through the application of chemical and NMR methods, the capsule, which defines the serotype specificity of the bacterium, was found to have the structure [structure: see text]. The O-polysaccharide (O-PS) component of the A. pleuro pneumoniae serotype 15 lipopolysaccharide (LPS) was characterized as a linear unbranched polymer of repeating pentasaccharide units composed of D-glucose (2 parts) and D-galactose (3 parts), shown to have the structure [structure: see text]. The O-PS was chemically identical with the O-antigen previously identified in the LPSs produced by A. pleuro pneumoniae serotypes 3 and 8.
authors.affiliation: Institute for Biological Science, National Research Council Canada, Ottawa, ON K1A 0R6, Canada. malcolm.perry@nrc-cnrc.gc.ca
authors.forename: Malcolm B
authors.initials: MB
authors.lastname: Perry
authors.forename: Leann L
authors.initials: LL
authors.lastname: MacLean
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000737:chemistry* / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D000942:Antigens, Bacterial / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D010759:Phosphorus Isotopes; D012703:Serotyping; D014461:Ultracentrifugation
chemical_list: D000942:Antigens, Bacterial; D002247:Carbon Isotopes; D019081:O Antigens; D010759:Phosphorus Isotopes