Preparation and characterization of C16 monolithic columns for capillary electrochromatography.
A series of methacrylamide-based C16 monolithic columns were prepared and characterized to determine how their porous structural properties and chromatographic behavior are affected by the percentages of functional monomer, base monomer, and cross-linker in the polymerization solution. Baseline separation of 6 neutral compounds can be readily obtained in an optimized column. Furthermore, the effects of organic additive in the mobile phase, operating voltages, and temperature on retention behaviors and separation efficiencies were also studied. The separation mechanism is also discussed. High column efficiency and good reproducibility indicate that the monolithic columns hold considerable promise.
Zhang
Kai
K
State Key Laboratory of Elemento-Organic Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China. zhangkai730@yahoo.com.cn
Yan
Chao
C
Yang
Junjiao
J
Zhang
Zhichao
Z
Wang
Qinsun
Q
Gao
Ruyu
R