Expression pattern of NOGO and NgR genes during human development.
Nogo protein has been identified as the component of central nervous system (CNS) myelin that limits axonal regeneration. We investigated the expression of the genes encoding Nogo and its receptor, NgR, between weeks eight and 23 of human embryonic development, by quantitative radioactive in situ hybridization. At 8 weeks, we detected NOGO and NgR transcripts in developing neuronal and non-neuronal structures. We focused on two different structures: the brain and the dental germs. During this period of development, NOGO and NgR transcripts colocalized in the cortical and ventricular zones of the brain, with expression strongest for these two genes in the postmitotic cells of the cortical plate. In developing dental germs, NgR was more strongly expressed than NOGO at 16 and 21 weeks. NOGO and NgR were expressed in zones of epithelium-mesenchyme interaction, which induce the differentiation of ameloblasts/odontoblasts. These genes were expressed most strongly in differentiated cells.
Al Halabiah
Hassan
H
Neurogénétique, INSERM E9935, Hôpital Robert Debré, AP-HP, 48 Boulevard Sérurier, 75019, Paris, France.
Delezoide
Anne-Lise
AL
Cardona
Ana
A
Moalic
Jean-Marie
JM
Simonneau
Michel
M
D001921:Brain / Q000196:embryology*; D047108:Embryonic Development; D047109:Fetal Development; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D008334:Mandible / Q000196:embryology; D009185:Myelin Proteins / Q000235:genetics*; D000070798:Nogo Proteins; D000070816:Nogo Receptor 1; D009805:Odontogenesis; D011956:Receptors, Cell Surface / Q000235:genetics*; D014158:Transcription, Genetic
D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070798:Nogo Proteins; D000070816:Nogo Receptor 1; C000603199:RTN4 protein, human; C486580:RTN4R protein, human; D011956:Receptors, Cell Surface