Role of nociceptin/orphanin FQ (Noc/oFQ) in murine experimental colitis.
Nociceptin/orphanin (Noc/oFQ), endogenous agonist for nociceptin receptor (NOR), is thought to be a stimulator of neurogenic inflammation. We investigated the possible role of Noc/oFQ in the development of colitis using NOR-deficient mice treated with dextran sulfate sodium (DSS). Colitis was significantly improved in NOR-deficient mice against wild-type mice. Expression level of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and infiltrating cells also significantly decreased in NOR-deficient mice against wild-type mice. Nociceptin expression increased in wild-type mice after DSS treatment. These results suggest stimulation by Noc/oFQ deteriorates colonic inflammation via up-regulation of adhesion molecule.
Kato
Shingo
S
Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan.
Tsuzuki
Yoshikazu
Y
Hokari
Ryota
R
Okada
Yoshikiyo
Y
Miyazaki
Junichi
J
Matsuzaki
Koji
K
Iwai
Atsuhiro
A
Kawaguchi
Atsushi
A
Nagao
Shigeaki
S
Itoh
Kazuro
K
Suzuki
Hidekazu
H
Nabeshima
Toshitaka
T
Miura
Soichiro
S
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D007150:Immunohistochemistry / Q000379:methods; D039641:Integrin beta Chains / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009088:Mucoproteins; D018847:Opioid Peptides / Q000172:deficiency / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D000097629:Nociceptin
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016264:Dextran Sulfate; D039641:Integrin beta Chains; D009088:Mucoproteins; D018847:Opioid Peptides; D000097629:Nociceptin; C489082:Madcam1 protein, mouse; C091368:integrin beta7