Structural basis for thermal stability of human low-density lipoprotein.
The stability of human low-density lipoprotein (LDL), the major cholesterol carrier in plasma, was analyzed by heating samples of different concentrations at a rate from 11 to 90 K/h. Correlation of the calorimetric, circular dichroism, fluorescence, turbidity, and electron microscopic data shows that thermal disruption of LDL involves irreversible changes in the particle morphology and protein conformation but no global protein unfolding. Heating to 85 degrees C induces LDL conversion into smaller and larger particles and apparent partial dissociation, but not unfolding, of its sole protein, apoB. Further heating leads to partial unfolding of the beta-sheets in apoB and to fusion of the protein-depleted LDL into large aggregated lipid droplets, resulting in a previously unidentified high-temperature calorimetric peak. These lipid droplets resemble in size and morphology the extracellular lipid deposits formed in the arterial wall in early atherosclerosis. The strong concentration dependence of LDL fusion revealed by near-UV/visible CD, turbidity, and calorimetry indicates high reaction order, and the heating rate dependence suggests high activation energy that arises from transient disruption of lipid and/or protein packing interactions in the course of particle fusion and apparent apoB dissociation. Consequently, thermal stability of LDL is modulated by kinetic barriers. Similar barriers may confer structural integrity to LDL subclasses in vivo.
Jayaraman
Shobini
S
Department of Physiology and Biophysics, W329, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA.
Gantz
Donald
D
Gursky
Olga
O
D001055:Apolipoproteins B / Q000737:chemistry; D001161:Arteriosclerosis / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D006358:Hot Temperature; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008027:Light; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D009391:Nephelometry and Turbidimetry; D010447:Peptide Hydrolases / Q000378:metabolism; D011489:Protein Denaturation; D017510:Protein Folding; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D010447:Peptide Hydrolases