[Oligonucleotide microarray for human immunodeficiency virus detection].
OBJECTIVE
To develop an oligonucleotide microarray for fast detection of human immunodeficiency virus (HIV).


METHODS
With complete genome sequence of HIV-1 subtype B (U26942) as the target sequence and bioinformatics software such as DNAClub, Oligo6.0, BLAST, Alignment, oligonucleotide probes of high specificity with identical length and similar melting temperature (T(m)) were designed and synthesized. Oligonucleotide microarray was prepared using Cartesian Microarrayer. Using the plasmids of HIV-1 subtype B(U26942), C(U46016), F(AF075703), G(AF061640) and restriction display technique, Cy3-labeled HIV DNA fragments were amplified and the hybridization results were scanned and analyzed with Array-Pro.


CONCLUSIONS
Twenty-two optimized oligonucleotide microarray probes were obtained and used to prepare the oligonucleotide microarray for further screening studies. The microarray prepared significantly enhanced the sensitivity, reliability and speed of DNA assay, and possesses the potential for application in clinical setting.
Wen
Ying
Y
Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. wenwen97@fimmu.com
Ma
Wen-Li
WL
Li
Ling
L
Wu
Qing-Hua
QH
Xu
Qiu-Lin
QL
Zhang
Hai-Yan
HY
Zheng
Wen-Ling
WL
D001483:Base Sequence; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity