Functional analysis of three plasmids from Lactobacillus plantarum.
Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the pWCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers.
van Kranenburg
Richard
R
Wageningen Centre for Food Sciences, Wageningen, The Netherlands.
Golic
Natasa
N
Bongers
Roger
R
Leer
Rob J
RJ
de Vos
Willem M
WM
Siezen
Roland J
RJ
Kleerebezem
Michiel
M
D001149:Arsenates / Q000494:pharmacology; D018053:Arsenites / Q000494:pharmacology; D001483:Base Sequence; D002874:Chromosome Mapping; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D018628:Gene Dosage; D005798:Genes, Bacterial; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012093:Replicon; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D001149:Arsenates; D018053:Arsenites; D004269:DNA, Bacterial; C015001:arsenite; C025657:arsenic acid