title: [In vitro effect on the differentiation and maturation of monocyte-derived dendritic cells by mannan-binding lectin].
abstract: OBJECTIVE
  To explore the effect of mannan-binding lectin (MBL) on the differentiation and maturation of human peripheral blood monocyte-derived dendritic cells (MoDCs).
  
  
  METHODS
  After MoDCs was stimulated with human natural MBL, MoDC's morphology was observed under inverted microscope and the expressions of CD1a, CD83, CD40, CD80, CD86 and HLA-DR on MoDCs were analyzed by FACS. The ability of MoDCs to stimulate the proliferation of allogenic T cells was detected by (3)H-TdR incorporation. The ability of MoDCs to up-take antigens was evaluated by zymosan granule phagocytosis test. The levels of IL-12 and TNF-alpha in the culture supernatant of MoDCs were determined by ELISA.
  
  
  RESULTS
  The expressions of CD1a, CD83, CD40, CD80, CD86 and HLA-DR on the MoDCs were up-regulated by MBL. The ability of MoDCs to up-take zymosan granules decreased and the proliferation of native T cells induced by MoDCs was enhanced. MBL stimulated the production of IL-12 by MoDCs, but had no such effect on TNF-alpha secretion.
  
  
  CONCLUSIONS
  MBL can induce differentiation and maturation of DCs in vitro, suggesting that MBL possibly participate in the adaptive immune response through modulation of functions of DCs.
authors.affiliation: Department of Immunology, Southern Medical University, Guangzhou 510515, China. chenyue@fimmu.com
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Zheng-liang
authors.initials: ZL
authors.lastname: Chen
authors.forename: Da-ming
authors.initials: DM
authors.lastname: Zuo
authors.forename: Yi-gang
authors.initials: YG
authors.lastname: Qiu
authors.forename: Li-yun
authors.initials: LY
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D037601:Mannose-Binding Lectin / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D015054:Zymosan / Q000378:metabolism
chemical_list: D037601:Mannose-Binding Lectin; D015054:Zymosan