title: [Utility of random amplified polymorphic DNA in the discrimination between Candida albicans and Candida dubliniensis].
abstract: Candida dubliniensisis a recently described species closely related to Candida albicans. Since the discrimination between both species by conventional mycological methods is not easy, many researchers have been trying DNA-related techniques in order to identify C. dubliniensis correctly. In this study, we propose the use of the random amplification of polymorphic DNA (RAPD) with a commercialized short primer which discriminates between both species. This oligonucleotide, AB1-12, allowed also separating C. albicans isolates into four different genotypes. These genotypes were different from the unique genotype observed in C. dubliniensis.
authors.affiliation: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco, Apartado 699, E-48080 Bilbao, España. oipquang@lg.ehu.es
authors.forename: G
authors.initials: G
authors.lastname: Quindós
authors.forename: R
authors.initials: R
authors.lastname: Alonso-Vargas
authors.forename: J
authors.initials: J
authors.lastname: Garaizar
authors.forename: J
authors.initials: J
authors.lastname: Pontón