Antegrade iliac artery stent implantation for the temporal and spatial examination of stent-induced neointimal hyperplasia and alterations in regional fluid dynamics.
BACKGROUND
Neointimal hyperplasia remains an important problem after stent implantation. Previous investigations examining vascular responses to stent implantation and effects of drugs have used a retrograde deployment approach that may inadvertently alter the local fluid dynamics surrounding the stent. We present a model of antegrade iliac artery stent implantation that facilitates the analysis of stent-induced alterations in neointimal hyperplasia and wall shear stress in vivo.


METHODS
Stent delivery catheters were inserted through the left carotid artery in anesthetized rabbits (n=37). Catheters were advanced under fluoroscopic guidance to the distal iliac arteries, where the stent was deployed. Hemotoxylin and eosin (H&E) staining of unstented and stented vascular sections was performed 21 days after implantation.


RESULTS
Selective unilateral stent implantation was successful in 32 of 37 rabbits. No histological abnormalities were observed in the aorta, contralateral unstented iliac, or distal femoral arteries. Neointimal hyperplasia was localized to the stented region.


CONCLUSIONS
The model of stent implantation was relatively easy to perform and produced selective neointimal hyperplasia within the stented region without evidence of damage, cellular proliferation, or flow disruption in the surrounding normal arterial vessels. The model will allow detailed examination of the influence of stent implantation on indices of wall shear stress, neointimal hyperplasia, the mechanisms of cellular proliferation in vivo, and their modification by drugs.
LaDisa
John F
JF
Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, USA.
Meier
Heidi T
HT
Olson
Lars E
LE
Kersten
Judy R
JR
Warltier
David C
DC
Pagel
Paul S
PS
D000818:Animals; D019917:Blood Vessel Prosthesis Implantation; D006965:Hyperplasia; D007083:Iliac Artery / Q000000981:diagnostic imaging / Q000473:pathology* / Q000502:physiology*; D008297:Male; D008955:Models, Cardiovascular; D011817:Rabbits; D011859:Radiography; D012039:Regional Blood Flow; D015607:Stents; D017539:Tunica Intima / Q000473:pathology*