[Application of molecular absorption spectrophotometric method to the determination of biologic macromolecular structures].
A review on recent applications of molecular absorption spectrophotometric method to the identification of the structures of biologic macromolecules, such as protein and nucleic acid, is presented. Molecular absorption spectrophotometric method is widely used in the structure determination of biologic macromolecules for its convenience and speed. Ultraviolet absorption spectrum (UV) can be used in the research on the mechanism of the interaction of anticancer drugs and DNA. UV can also be used to study the interaction of spectroscopic probe with biologic molecule and their binding mechanism. Protein secondary structure and conformation can be investigated by Fourier infrared spectroscopy (FTIR) deconvolution analysis. Molecular absorption spectrophotometric method is an important tool for structure study of biologic macromolecules.
Cao
Shu-xia
SX
Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China.
Zhao
Yu-fen
YF
D001665:Binding Sites; D002942:Circular Dichroism / Q000379:methods*; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D004063:Digestion; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman
D046911:Macromolecular Substances; D004247:DNA