title: Dengue virus detection by using reverse transcription-polymerase chain reaction in saliva and progeny of experimentally infected Aedes albopictus from Brazil.
abstract: Oral susceptibility and vertical transmission of dengue virus type 2 (DENV-2) in an Aedes albopictus sample from Rio de Janeiro was estimated. The infection (36.7%) and transmission (83.3%) rates for Ae. albopictus were higher than those of an Ae. aegypti colony used as control, 32.8 and 60%, respectively. Fourth instar larvae and females descendants of 48.5 and 39.1% of experimentally infected Ae. albopictus showed to harbor the virus. The oral susceptibility and the high capacity to assure vertical transmission exhibited by Ae. albopictus from Brazil reinforce that this species may play a role in the maintenance of the virus in nature and be a threat for dengue control in the country.
authors.affiliation: Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. mcastro@ioc.fiocruz.br
authors.forename: Márcia Gonçalves de
authors.initials: MG
authors.lastname: Castro
authors.forename: Rita Maria Ribeiro
authors.initials: RM
authors.lastname: Nogueira
authors.forename: Hermann Gonçalves
authors.initials: HG
authors.lastname: Schatzmayr
authors.forename: Marize Pereira
authors.initials: MP
authors.lastname: Miagostovich
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lourenço-de-Oliveira
mesh_terms: D000330:Aedes / Q000235:genetics / Q000821:virology*; D000818:Animals; D001938:Brazil; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007303:Insect Vectors / Q000235:genetics / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva / Q000821:virology*; D013045:Species Specificity
chemical_list: D012367:RNA, Viral