Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis.
Intraspecies variation of Chromobacterium violaceum was examined by comparative sequence - and by restriction fragment length polymorphism analysis of the recombinase A gene (recA-PCR-RFLP). Primers deduced from the known recA gene sequence of the type strain C. violaceum ATCC 12472(T) allowed the specific amplification of a 1040bp recA fragment from each of the 13 C. violaceum strains investigated, whereas other closely related organisms tested negative. HindII-PstI-recA RFLP analysis generated from 13 representative C. violaceum strains enabled us to identify at least three different genospecies. In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level.
Scholz
Holger Christian
HC
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany. holger1scholz@bundeswehr.org
Witte
Angela
A
Tomaso
Herbert
H
Al Dahouk
Sascha
S
Neubauer
Heinrich
H
D015373:Bacterial Typing Techniques; D002861:Chromobacterium / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011938:Rec A Recombinases / Q000235:genetics*
D004269:DNA, Bacterial; D011938:Rec A Recombinases