Apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods.
This paper presents results from a study to develop cryopreservation procedures for apices of several strawberry genotypes. Five Fragaria x ananassa Duch. cultivars and two wild species (F. chiloensis and F. virginiana) have been screened using the encapsulation-dehydration method and/or a protocol which compromises vitrification and encapsulation-dehydration. Apices were encapsulated in an alginate gel, precultured on media containing high levels of sucrose (0.8 M, conventional protocol), or a combination of 0.4 M sucrose and 2 M glycerol. Recovery rates varied among genotypes (23-63%). The latter method reduced considerably the time needed for the cryogenic procedure by eliminating the pre-treatment with 0.8 M sucrose for 19 h prior to dehydration, as required by the conventional procedure.
Clavero-Ramírez
I
I
Centro de Investigación y Formación Agraria de Málaga, IFAPA, Consejería de Innovación, Ciencia y Empresas, Junta de Andalucía, 20140-Churriana, Málaga, Spain.
Gálvez-Farfán
J
J
López-Aranda
J M
JM
González-Benito
M E
ME
D002470:Cell Survival; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents / Q000008:administration & dosage; D003890:Desiccation / Q000379:methods; D031985:Fragaria / Q000235:genetics* / Q000254:growth & development; D005838:Genotype; D005854:Germ Cells / Q000187:drug effects / Q000254:growth & development; D018514:Plant Structures / Q000235:genetics / Q000254:growth & development
D003451:Cryoprotective Agents