[A novel catalysis kinetics fluorimetry system for the determination of eliminating ratio of Chinese traditional medicine for *OH].
Benzoic acid with weak fluorescence may react on *OH, and then products with intense fluorescence are made. Extractives of Chinese traditional medicine may eliminate *OH in solution, and reduce the amounts of the products. Then, the increased level of fluorescence of the products in solution will be lowered. Based on this principle, a novel catalysis kinetics fluorimetry system for the determination of eliminating ratio of Chinese traditional medicine for *OH was developed. When the concentration of traditional Chinese herbal drugs was 4.0 mg (dry weight) x mL(-1), the eliminating ratios of Viola yedoensis, Atrctylodes chinensis and Paeonia veitchii were 60.8%, 40.1% and 94.3%, respectively. The results obtained by this method are in good agreement with those obtained by spectrophotometry.
Qu
Peng
P
Department of Chemistry, Shangqiu Normal College, Shangqiu 476000, China.
Li
Guan-liang
GL
Xu
Mao-tian
MT
D019817:Benzoic Acid / Q000737:chemistry*; D002384:Catalysis; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D005470:Fluorometry / Q000379:methods*; D005609:Free Radicals; D007700:Kinetics; D008516:Medicine, Chinese Traditional
D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D019817:Benzoic Acid