3(10)-Helix adjoining alpha-helix and beta-strand: sequence and structural features and their conservation.
Does the amino acid use at the terminal positions of an alpha-helix become altered depending on the context-more specifically, when there is an adjoining 3(10)-helix, and can a single helical cylinder encompass the resultant composite helix? An analysis of 138 and 107 cases of 3(10)-alpha and alpha-3(10) composite helices, respectively, found in known protein structures indicate that the secondary structural element occurring first imposes its characteristics on the sequence of the structural element coming next. Thus, when preceded by a 3(10)-helix, the preference of proline to occur at the N1 position of an alpha-helix is shifted to the N2 position, a typical characteristic of the C-terminal capping of the 3(10)-helix. When an alpha- or a 3(10)-helix leads into a helix of the other type, there is a bend at the junction, especially for the 3(10)-alpha composite, with the two junction residues facing inward and buried within the structure. Thus a single helical cylinder may not properly represent a composite helix, the bend providing a means for the tertiary structure to assume a globular shape, very much akin to what a proline-induced kink does to an alpha-helix. The tertiary structural context in which beta-3(10) and 3(10)-beta composites occurs can be different, causing the angle between the secondary structural elements in the two cases to be different. Composites of 3(10)-helices and beta-strands are much more conserved among members in families of homologous structures than those between two types of helices; in many of the former instances, the 3(10)-helix constitutes the loops in beta-hairpin or beta-beta-corner motifs. The overall fold of the chain may be more conserved than the actual identify of the secondary structure elements in a composite.
Pal
Lipika
L
Bioinformatics Centre, Bose Institute, P-1/12 CIT Scheme VIIM, Calcutta 700 054, India.
Dasgupta
Bhaskar
B
Chakrabarti
Pinak
P
D000595:Amino Acid Sequence; D019295:Computational Biology; D003198:Computer Simulation; D017124:Conserved Sequence; D019143:Evolution, Molecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary