PDGF-D contributes to neointimal hyperplasia in rat model of vessel injury.
In this study, we determined the role of PDGF-D, a new member of the PDGF family, in a rat model of balloon injured artery made with a 2F catheter in Sprague-Dawley male rats. PDGF-D expression was studied in the injured and control segments of abdominal aorta. The function of PDGF-D was evaluated in rat vascular smooth muscle cells stably transfected with PDGF-D gene. We found that in normal abdominal aorta, PDGF-D was highly expressed in adventia, moderate in endothelia, and unidentified in media. Stable transfection of PDGF-D gene into vascular smooth muscle cells increased the cell migration by 2.2-fold, and the proliferation by 2.3-fold, respectively, and MMP-2 production and activity as well. These results support the fact that PDGF-D is involved in the formation of neointimal hyperplasia induced by balloon catheter injury and may serve as a target in preventing vascular restenosis after coronary angioplasty.
Chen
Jingzhou
J
Sino-German Laboratory for Molecular Medicine and Hypertension Division, Fuwai Hospital, Chinese Academy of Medical Sciences and National Genome Center, Beijing, PR China.
Han
Yu
Y
Lin
Chunxia
C
Zhen
Yisong
Y
Song
Xiaodong
X
Teng
Siyong
S
Chen
Chen
C
Chen
Yu
Y
Zhang
Yinhui
Y
Hui
Rutai
R
D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006083:Graft Occlusion, Vascular / Q000378:metabolism* / Q000473:pathology*; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D008222:Lymphokines / Q000378:metabolism*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017539:Tunica Intima / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology
D008222:Lymphokines; C498952:Pdgfd protein, rat; D010982:Platelet-Derived Growth Factor