[Resonance light scattering study on the interaction of Victory blue-B with deoxyribonucleic acid and application to DNA assay].
The enhancement of the Resonance Light Scattering (RLS) due to Victory blue-B on the molecular surface of DNA in alkalescence solution was studied. A method of resonance light scattering was developed to determine DNA. Deoxyribonucleic acid (DNA) was studied in the range of pH 9.0-10.5. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of DNA at nanogram level based on RLS measurement. The linear range of the DNA is 0-3.0 mg x L(-1) with a correlation coefficient of 0.9978. The detection limit is 21.5 microg x L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.
Su
Jie-shu
JS
Institute of Chemistry, Xiangtan University, Xiangtan 411105, China.
Chen
Xiao-ming
XM
Long
Yun-fei
YF
Li
Wei
W
Luo
Hai-tang
HT
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008027:Light; D057230:Limit of Detection; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014732:Vibration
D004247:DNA