title: Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination.
abstract: The simple and rapid RP-HPLC method, for the simultaneous determination of lidocaine and cetrimonium bromide in the presence of pellet color corrigent, was developed. Separations were performed on a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5 microm particle column at 40 degrees C. The mobile phase consisted of water phase and acetonitrile (72:28 V/V), pH value of the mobile phase was adjusted to 2.0 with 85% ortophosphoric acid. Bisacodil was used as an internal standard. The flow rate was 1 ml/min and UV detection was performed at 208 nm. The proposed RP-HPLC method was validated and all the parameters for the validation of the method are given. According to the obtained results, the developed method was found to be suitable and accurate for the determination of these drugs in commercial formulations.
authors.affiliation: Department of Drug Analysis, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia and Montenegro.
authors.forename: A
authors.initials: A
authors.lastname: Malenovic
authors.forename: M
authors.initials: M
authors.lastname: Medenica
authors.forename: D
authors.initials: D
authors.lastname: Ivanovic
authors.forename: B
authors.initials: B
authors.lastname: Jancic
authors.forename: S
authors.initials: S
authors.lastname: Markovic
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008012:Lidocaine / Q000032:analysis*; D004364:Pharmaceutical Preparations; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000097:Acetonitriles; D002593:Cetrimonium Compounds; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D008012:Lidocaine; C032159:acetonitrile; D000077286:Cetrimonium