The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo.
Maternal and zygotic activities of the homeodomain protein PAL-1 specify the identity and maintain the development of the multipotent C blastomere lineage in the C. elegans embryo. To identify PAL-1 regulatory target genes, we used microarrays to compare transcript abundance in wild-type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomeres. pal-1-dependent C-lineage expression was verified for select candidate target genes by reporter gene analysis, though many of the target genes are expressed in additional lineages as well. The set of validated target genes includes 12 transcription factors, an uncharacterized wingless ligand and five uncharacterized genes. Phenotypic analysis demonstrates that the identified PAL-1 target genes affect specification, differentiation and morphogenesis of C-lineage cells. In particular, we show that cell fate-specific genes (or tissue identity genes) and a posterior HOX gene are activated in lineage-specific fashion. Transcription of targets is initiated in four temporal phases, which together with their spatial expression patterns leads to a model of the regulatory network specified by PAL-1.
Baugh
L Ryan
LR
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Hill
Andrew A
AA
Claggett
Julia M
JM
Hill-Harfe
Kate
K
Wen
Joanne C
JC
Slonim
Donna K
DK
Brown
Eugene L
EL
Hunter
Craig P
CP
D000704:Analysis of Variance; D000818:Animals; D001757:Blastomeres / Q000502:physiology*; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000378:metabolism*; D046228:Microarray Analysis; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D034622:RNA Interference; D015534:Trans-Activators / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; D018398:Homeodomain Proteins; D015534:Trans-Activators; C068665:pal-1 protein, C elegans