Detection of multiple cephalosporin-resistant Escherichia coli from a cattle fecal sample in Great Britain.
We describe the isolation of multiple cephalosporin-resistant Escherichia coli from cattle feces collected from animals at slaughter in Great Britain. Six E. coli strains were isolated with distinct XbaI pulsed-field gel electrophoresis (PFGE) profiles and different mechanisms of cephalosporin resistance from a single fecal sample. Two of these strains were found to contain conjugative plasmids conferring resistance to extended-spectrum cephalosporins that were indistinguishable from each other by restriction endonuclease digestion. Sequence analysis of the plasmid-encoded ampC showed that they were identical to bla(CMY-2), previously described in multiple-drug-resistant Salmonella and E. coli from animals in other parts of the world. DNA sequence analysis of the chromosomal ampC promoter regions for three cephalosporin-resistant strains lacking CMY-2 was determined. Several mutations were detected in the isolates tested including changes at positions -42 and -32, which are known to increase promoter strength. This report represents the first isolation of E. coli containing bla(CMY-2) from cattle in Great Britain, and, also to our knowledge, the first demonstration of multiple cephalosporin-resistant strains in a single animal.
Batchelor
M
M
Food and Environmental Safety Department, Veterinary Laboratories Agency, Addlestone, Surrey, KT15 3NB, UK.
Clifton-Hadley
F A
FA
Stallwood
A D
AD
Paiba
G A
GA
Davies
R H
RH
Liebana
E
E
D000818:Animals; D002417:Cattle; D018550:Cephalosporin Resistance / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D005243:Feces / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D006113:United Kingdom
D002511:Cephalosporins