Sol i 1, the phospholipase allergen of imported fire ant venom.
BACKGROUND
Sol i 1, the venom phospholipase of imported fire ant venom is an important allergen and exhibits some cross-reactivity with IgE antibodies from patients sensitized to other Hymenoptera venoms.


OBJECTIVE
To determine the primary structure of Sol i 1 and evaluate the roles of protein and carbohydrate epitopes in its cross-reactivity.


METHODS
Sol i 1 was purified from venom, proteolytic peptides prepared and amino acid sequences obtained. The cDNA for Sol i 1 was cloned, sequenced, and compared with sequences of other wasp venom phospholipases. The role of carbohydrate epitopes in the cross-reactivity with other Hymenoptera venoms was studied by RAST inhibition.


RESULTS
The sequence identified Sol i 1 as a lipase of the GX class, lipoprotein lipase superfamily, pancreatic lipase homologous family and RP2 subgroup phospholipases as are the vespid venom phospholipases. The 148 residues identified by amino acid sequencing represent about 48% of the translated cDNA sequence. Sol i 1 was 31-32% identical to yellow jacket phospholipases. The identical regions of sequence were clustered in the domain which forms the serine hydrolase active site. Mannosylated N-glycans could completely inhibit binding of IgE from honeybee venom sensitized patients to Sol i 1. Inhibition by glycan of IgE binding from yellow jacket venom sensitized patients was low or absent for three of eight sera and substantial, but not complete for five sera.


CONCLUSIONS
Sol i 1 is related to wasp venom phospholipases. Cross-reactivity with honeybee venom is caused by carbohydrate, whereas cross-reactivity with yellow jacket venom involves reactivity with both carbohydrate determinants of hyaluronidase and high molecular weight proteins and phospholipase protein determinants.
Hoffman
Donald R
DR
Department of Pathology and Laboratory Medicine, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA. hoffmand@mail.ecu.edu
Sakell
Rhonda H
RH
Schmidt
Margit
M
D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000862:Ant Venoms / Q000737:chemistry*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D010740:Phospholipases / Q000737:chemistry* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011852:Radioallergosorbent Test; D017386:Sequence Homology, Amino Acid; D014862:Wasp Venoms / Q000737:chemistry
D000485:Allergens; D000862:Ant Venoms; D019476:Insect Proteins; D014862:Wasp Venoms; D010740:Phospholipases