title: Radiolabelling of egg meals for gastric emptying studies: a comparison of 99mTc sulfur and 99mTc stannous colloids.
abstract: OBJECTIVE
  To establish whether 99mTc stannous (Tc-Sn) colloid is a suitable alternative to 99mTc sulfur (Tc-S) colloid for gastrointestinal studies, we compared the per cent binding to egg solids (%BS) and radiochemical purity (RCP) of both colloids in digesting media.
  
  
  METHODS
  Egg white and yolk containing colloids were cooked separately and mashed. Samples of 4-5 g were digested over 2-3 h (37 degrees C) in excess simulated gastric juice (SGJ: 15 ml of 0.1 M HCl and 0.5 g.l-1 pepsin) or water, centrifuged, imaged with a gamma camera and the %BS computed. RCP was determined in aspirates taken from these preparations and from solutions of colloid added directly to SGJ.
  
  
  RESULTS
  The %BS in egg white after 3 h in SGJ for both colloids were similar: Tc-Sn, 62+/-8 (n=12); Tc-S, 61+/-6 (n=8), but markedly lower than 95% (the often quoted literature value). Egg yolk was digested more rapidly than egg white: %BS after 2 h in SGJ for Tc-Sn colloid was 55+/-10 (n=5) in the yolk, compared to 77+/-4 (n=5) in the white (P<0.01). The RCP for Tc-S colloid alone in SGJ was >94% over 3 h but for Tc-Sn colloid was as low as 14%. For egg white, the RCP in SGJ was 91-96% for Tc-S and 80-91% for Tc-Sn. For egg yolk the RCPs in SGJ were similar for both colloids (>90%). The RCP in water digesting egg white or yolk containing either colloid was always lower than in the corresponding SGJ aspirates, indicating a leakage of small amounts of non-colloidal 99mTc.
  
  
  CONCLUSIONS
  Although 99mTc-Sn almost completely dissociates in SGJ, once cooked in egg it is digested similarly to 99mTc-S. Variations in the size of digesting egg fragments and in SGJ composition can reconcile the lower %BS values obtained with previously reported higher results. 99mTc-Sn colloid cooked in egg appears suitable for gastrointestinal studies.
authors.affiliation: Department of Diagnostic Imaging, National University Hospital, Singapore. dnrsb@nus.edu.sg
authors.forename: Borys
authors.initials: B
authors.lastname: Shuter
authors.forename: Siok-Mei
authors.initials: SM
authors.lastname: Ng
mesh_terms: D004531:Eggs / Q000032:analysis*; D005746:Gastric Emptying / Q000502:physiology; D005766:Gastrointestinal Contents / Q000737:chemistry* / Q000000981:diagnostic imaging*; D006207:Half-Life; D007553:Isotope Labeling / Q000379:methods*; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000032:analysis / Q000737:chemistry; D017556:Technetium Compounds / Q000032:analysis / Q000737:chemistry*; D013671:Technetium Tc 99m Sulfur Colloid / Q000032:analysis / Q000737:chemistry*; D017971:Tin Compounds / Q000032:analysis / Q000737:chemistry*
chemical_list: D019275:Radiopharmaceuticals; D017556:Technetium Compounds; D017971:Tin Compounds; C033493:technetium Tc 99m tin colloid; D013671:Technetium Tc 99m Sulfur Colloid