title: Histogenetic phenotypes of B cells in posttransplant lymphoproliferative disorders by immunohistochemical analysis correlate with transplant type: solid organ vs hematopoietic stem cell transplantation.
abstract: We immunohistochemically defined the histogenesis of posttransplantation lymphoproliferative disorders (PTLDs; B-cell phenotype) occurring after allogeneic T cell-depleted hematopoietic stem cell transplantation (HSCT; n = 15) or solid organ transplantation (SOT; n = 11) to determine whether transplantation type or morphologic subtype of PTLD affected the histogenetic subtype. Immunohistochemical stains using histogenetic markers for germinal center (GC) B cells, late GC and post-GC B cells, and post-GC B cells were performed on paraffin-embedded samples. Morphologically, 14 cases were polymorphic; 12 were monomorphic. Histogenetic marker expression was as follows: 1 monomorphic case (4%), GC phenotype expressing bcl-6 and CD10; 17 cases (65%; polymorphic, 9; monomorphic, 8), late GC-early post-GC phenotype expressing MUM1/IRF4; 8 cases (31%; polymorphic, 5; monomorphic, 3), post-GC phenotype expressing MUM1/IRF4 and CD138 but not bcl-6. PTLD cases after HSCT more frequently were post-GC phenotype than after SOT (7/15 vs 1/11, respectively; P = .040) and were independent of morphologic subclassification. Results suggest that most PTLDs are late GC-early post-GC phenotype with a minor group of post-GC phenotype and rare cases of GC phenotype. Findings also suggest a correlation between histogenetic phenotype of B-cell PTLD and type of transplantation.
authors.affiliation: Department of Pathology, Medical College of Wisconsin, Milwaukee, USA.
authors.forename: Louis
authors.initials: L
authors.lastname: Novoa-Takara
authors.forename: Sherrie L
authors.initials: SL
authors.lastname: Perkins
authors.forename: Dan
authors.initials: D
authors.lastname: Qi
authors.forename: Vinod B
authors.initials: VB
authors.lastname: Shidham
authors.forename: David H
authors.initials: DH
authors.lastname: Vesole
authors.forename: Sundaram
authors.initials: S
authors.lastname: Hariharan
authors.forename: Yamin
authors.initials: Y
authors.lastname: Luo
authors.forename: April
authors.initials: A
authors.lastname: Ewton
authors.forename: Chung-Che
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002648:Child; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008232:Lymphoproliferative Disorders / Q000145:classification / Q000276:immunology / Q000473:pathology*; D008297:Male; D016377:Organ Transplantation / Q000009:adverse effects*; D011183:Postoperative Complications / Q000145:classification / Q000276:immunology / Q000473:pathology*; D012189:Retrospective Studies