Secretory expression of thermostable T1 lipase through bacteriocin release protein.
The extracellular production of T1 lipase was performed by co-expression of pJL3 vector encoding bacteriocin release protein in prokaryotic system. Secretory expression was optimized by considering several parameters, including host strains, inducer (IPTG) concentration, media, induction at A(600 nm), temperature, and time of induction. Among the host strains tested, Origami B excreted out 18,100 U/ml of lipase activity into culture medium when induced with 50 microM IPTG for 12 h. The Origami B harboring recombinant plasmid pGEX/T1S and pJL3 vector was chosen for further study. IPTG at 0.05 mM, YT medium, induction at A(600 nm) of 1.25, 30 degrees C, and 32 h of induction time were best condition for T1 lipase secretion with Origami B as a host.
Rahman
Raja Noor Zaliha Raja Abdul
RN
Faculty of Biotechnology and Biomolecular Sciences, Enzyme and Microbial Technology Research Group, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia. rnzaliha@fsas.upm.edu.my
Leow
Thean Chor
TC
Basri
Mahiran
M
Salleh
Abu Bakar
AB
D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D005822:Genetic Vectors; D007544:Isopropyl Thiogalactoside; D008049:Lipase / Q000235:genetics*; D010957:Plasmids; D013696:Temperature; D013995:Time
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C052368:cloacin DF13-encoded bacteriocin release protein, E coli; D007544:Isopropyl Thiogalactoside; D008049:Lipase; C428386:thermostable lipase