Expression of calsenilin in neurons and astrocytes in the Alzheimer's disease brain.
Calsenilin, a multifunctional Ca2+-binding protein, has been identified as an Alzheimer's disease-associated presenilin interactor. Here, we investigated the histochemical localization of calsenilin and its expression levels in the brains of sporadic Alzheimer's disease. Both messenger RNA and protein expression of calsenilin were observed in neurons of the cerebral cortex and hippocampus of control brains, and more intense staining was in Alzheimer's disease brains. Although calsenilin is primarily expressed in neurons, its immunoreactivity was also detected in reactive astrocytes of the Alzheimer's disease brains. In Alzheimer's disease brains, the caspase-derived fragment of calsenilin was only detected in cytosolic fraction. Our findings suggest that calsenilin overexpression in both neurons and reactive astrocytes may play an important role in apoptosis and in Alzheimer's disease pathology.
Jin
Jae-Kwang
JK
Ilsong Institute of Life Science, Hallym University, Anyang, Kyonggi-do 431-060, Korea.
Choi
Jin-Kyu
JK
Wasco
Wilma
W
Buxbaum
Joseph D
JD
Kozlowski
Piotr B
PB
Carp
Richard I
RI
Kim
Yong-Sun
YS
Choi
Eun-Kyoung
EK
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D001253:Astrocytes / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D051605:Kv Channel-Interacting Proteins; D008297:Male; D009474:Neurons / Q000378:metabolism*; D011180:Postmortem Changes; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D002135:Calcium-Binding Proteins; C496749:KCNIP3 protein, human; D051605:Kv Channel-Interacting Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases