title: A change in gastric mucosal ascorbic acid status with the formation, progression, and recovery of compound 48/80-induced acute gastric mucosal lesions in rats.
abstract: We examined whether gastric mucosal ascorbic acid status changes with the formation, progression, and recovery of acute gastric mucosal lesions in rats treated with compound 48/80, a mast cell degranulator. Fasted Wistar rats received a single intraperitoneal injection of compound 48/80 (0.75 mg/kg). Apparent gastric mucosal lesions occurred 0.5 h after compound 48/80 treatment, progressed gastric mucosal lesions were observed at 3 h, and a partial recovery of the progressed lesions was found at 6 h. The gastric mucosal concentrations of total and reduced ascorbic acids in compound 48/80-treated rats decreased to approximately 60% of the levels of untreated rats at 3 h after the treatment but the decreased concentrations of total and reduced ascorbic acids were almost completely returned to the levels of untreated rats at 6 h. The gastric mucosal concentration of oxidized ascorbic acid in compound 48/80-treated rats showed little change. The serum concentrations of total and reduced ascorbic acids in compound 48/80-treated rats increased at 0.5 h after the treatment and further increased at 3 h but the increased concentrations of total and reduced ascorbic acids were almost completely returned to the levels of untreated rats at 6 h. The serum concentration of oxidized ascorbic acid in compound 48/80-treated rats increased transiently at 0.5 h after the treatment. The hepatic concentrations of total. reduced, and oxidized ascorbic acids in compound 48/80-treated rats increased 3 h after the treatment, but these increases were not observed at 6 h. These results indicate that gastric mucosal ascorbic acid status is disrupted with the progression of acute gastric mucosal lesions in rats treated with compound 48/80.
authors.affiliation: Department of Chemistry, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan. yohta@fujita-hu.ac.jp
authors.forename: Yoshiji
authors.initials: Y
authors.lastname: Ohta
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Imai
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Arisawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakano
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis* / Q000097:blood; D005753:Gastric Mucosa / Q000737:chemistry* / Q000473:pathology; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013272:Stomach Diseases / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D003189:p-Methoxy-N-methylphenethylamine; D001205:Ascorbic Acid