[Identification of Rad51 protein from Chlamydomonas reinhardtii: recombinational characteristics].
The unicellular green microalga Chlamydomonas reinhardtii is a perspective model object for basic and applied research. However, its homologous recombination (HR) system which lies in the basis of double-strand DNA break repair have still not been studied. Last years the program of C. reinhardtii nuclear genome sequence is realized and different nucleotide repeats in the genome structure have been revealed that can explain a low level of HR relative to nonhomologous recombination events. Analyses of the C. reinhardtii EST (Expressed Sequence Tag)--and genome libraries permitted us to reconstruct and clone cDNA of the RAD51 gene. In present work, the cDNA was expressed, its product was purified and some basal biochemical activities were studied. The results show that Rad51C protein from lower eukaryote C. reinhardtii is identified as typical representative of the Rad51C-like subfamily of higher eukaryotes.
Shalguev
V I
VI
Kaboev
O K
OK
Sizova
I A
IA
Hegemann
P
P
Lantsov
V A
VA
D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics; D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020224:Expressed Sequence Tags; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D051135:Rad51 Recombinase; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic
D048028:DNA, Algal; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D051135:Rad51 Recombinase