Direct in vivo protein transduction into a specific restricted brain area in rats.
Attempts at protein transduction into specific restricted brain areas have remained unsuccessful. We attempted targeted, direct in vivo protein transduction by microinjecting beta-galactosidase (beta-gal) with hemagglutinating virus of Japan envelope (HVJ-E) vector into the rat nucleus tractus solitarius (NTS). The medulla oblongata including the NTS was removed 6h post-injection and cryostat sections were histochemically stained to detect beta-gal enzymatic activity. beta-gal-positive cells were present in these sections as was beta-gal activity determined by colorimetric analysis. beta-gal-positive cells were not present in the rats microinjected only beta-gal protein without HVJ-E vector. Our findings suggest that direct in vivo protein transduction into specific restricted brain areas is possible. The type of targeted delivery system we present may have wide applications in the administration of therapeutic proteins to the central nervous system.
Owada-Makabe
Kyoko
K
Department of Physiology, Wakayama Medical University School of Medicine, Wakayama, Japan.
Tsubota
Yuji
Y
Yukawa
Kazunori
K
Kakimoto
Nobuyuki
N
Liang
Xiang-Miang
XM
Ichinose
Masakazu
M
Maeda
Masanobu
M
D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D007150:Immunohistochemistry; D008297:Male; D008845:Microinjections; D051381:Rats; D029082:Sendai virus; D017552:Solitary Nucleus / Q000187:drug effects* / Q000378:metabolism; D001616:beta-Galactosidase / Q000008:administration & dosage* / Q000096:biosynthesis
D001616:beta-Galactosidase