[ABO genotyping by duplex amplification and oligonucleotide arrays assay].
OBJECTIVE
ABO genotyping for forensic identification by oligonucleotide chip.


METHODS
Oligonucleotide microarrays which could detect 3 different SNPs in exon 6 and exon 7 for ABO genotyping were used. Population studies on ABO was carried out in a sample of 115 unrelated Chinese Han individuals. The method was also applied to cases.


RESULTS
The technique could identify 6 genotypes of ABO system. According to the results of population studies, no significant deviations from Hardy-Weinberg equilibrium could be found. The observed and expected heterozygosities were 0.591 and 0.616 respectively. The polymorphic information content was 0.544. The average exclusion probabilities in buos and trios was 0.188 and 0.344 respectively. The discrimination power is 0.777.


CONCLUSIONS
The data and case application demonstrated that ABO typing by oligonucleotide probe arrays was a useful technique for paternity testing and individual identification.
Li
Li
L
Institute of Forensic Sciences, Ministry of Justice, PRChina, Shanghai 200063, China.
Li
Cheng-tao
CT
Li
Rong-yu
RY
Sun
Meiqian
M
Liu
Yan
Y
Li
Yao
Y
Lin
Yuan
Y
Que
Tingzhi
T
Cheng
Dalin
D
Yan
Pinhua
P
Fang
Jianxin
J
Zhao
Zhenmin
Z
Shen
Min
M
Du
Zhichun
Z
D000017:ABO Blood-Group System / Q000235:genetics*; D001801:Blood Stains; D004247:DNA / Q000097:blood; D017931:DNA Primers; D005260:Female; D005554:Forensic Medicine; D005838:Genotype; D006197:Hair / Q000737:chemistry; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
D000017:ABO Blood-Group System; D017931:DNA Primers; D004247:DNA