Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process.
A polychlorophenol-degrading strain, designated MT1(T), and three MT1-like strains, MT101, MT103 and MT104, were isolated from a cold (4-8 degrees C) fluidized-bed process treating chlorophenol-contaminated groundwater in southern Finland. The organisms were Gram-negative, rod-shaped, catalase-positive, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belonged to the alpha-4 subclass of the Proteobacteria and were members of the genus Novosphingobium. The highest 16S rRNA gene sequence similarity observed for these strains was 96.5 % with the type strains of Novosphingobium hassiacum, Novosphingobium aromaticivorans and Novosphingobium subterraneum. Chemotaxonomic data (major ubiquinone: Q-10; major polyamine: spermidine; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; major fatty acids: 18 : 1omega7c, 16 : 1omega7c and 2-OH 14 : 0) as well as the ability to reduce nitrate supported the affiliation of the strains to the genus Novosphingobium. Based on the phylogenetic analysis, whole-cell fatty acid composition as well as biochemical and physiological characteristics, the MT1-like strains were highly similar and could be separated from all recognized Novosphingobium species. The novel species Novosphingobium lentum sp. nov. is proposed to accommodate strains MT1(T) (=DSM 13663(T)=CCUG 45847(T)), MT101 (=CCUG 45849), MT103 (=CCUG 45850) and MT104 (=CCUG 45851).
Tiirola
Marja A
MA
Department of Biological and Environmental Science, University of Jyväskylä, FIN-40014 Jyväskylä, Finland.
Busse
Hans-Jürgen
HJ
Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Vienna, Austria.|Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Vienna, Austria.
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Männistö
Minna K
MK
Finnish Forest Research Institute, Eteläranta 55, FIN-96300 Rovaniemi, Finland.
D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism*; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005387:Finland; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D002733:Chlorophenols; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical