Novel dephosphotetrahydromethanopterin biosynthesis genes discovered via mutagenesis in Methylobacterium extorquens AM1.
Methylobacterium extorquens AM1 was used to explore the genetics of dephosphotetrahydromethanopterin (dH(4)MPT) biosynthesis. Strains with mutations in eight "archaeal-type" genes linked on the chromosome of M. extorquens AM1 were analyzed for the ability to synthesize dH(4)MPT, and six were found to be dH(4)MPT negative. Putative functions of these genes in dH(4)MPT biosynthesis are discussed.
Chistoserdova
Ludmila
L
231 Wilcox Hall, Box 352125, Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA. milachis@u.washington.edu
Rasche
Madeline E
ME
Lidstrom
Mary E
ME
D003067:Coenzymes / Q000096:biosynthesis* / Q000235:genetics; D020601:Methylobacterium extorquens / Q000235:genetics* / Q000378:metabolism*; D015394:Molecular Structure; D016296:Mutagenesis; D016366:Open Reading Frames; D011622:Pterins / Q000378:metabolism*
D003067:Coenzymes; D011622:Pterins; C499991:dephosphotetrahydromethanopterin