title: Acceptable performance of VITEK 2 system to detect extended-spectrum beta-lactamases in clinical isolates of Escherichia coli: a comparative study of phenotypic commercial methods and NCCLS guidelines.
abstract: The disk approximation method, Etest (AB Biodisk, Solna, Sweden), and VITEK 2 system (bioMérieux, Marcy l'Etoile, France) were used to study 399 extended-spectrum beta-lactamase (ESBL)-producing (115 strains) and non-ESBL-producing (284 strains) clinical isolates of Escherichia coli after recommended procedures. Comparative study of the phenotypic findings yielded data on the sensitivity, specificity, and positive and negative predictive values and performance of each method. The sensitivity (100% using 2 substrates), specificity (99.3-100%), and predictive values of the disk approximation, Etest, and VITEK 2 methods were similar.
authors.affiliation: Department of Microbiology, School of Medicine, San Cecilio University Hospital, University of Granada, E-18012 Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Sorlózano
authors.forename: José
authors.initials: J
authors.lastname: Gutiérrez
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Piédrola
authors.forename: María José
authors.initials: MJ
authors.lastname: Soto
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D010641:Phenotype; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases