[Proliferation and ALPase activity of human dental pulp cells in response to lipopolysaccharide in vitro].
OBJECTIVE
To investigate the effects of LPS on proliferation and ALPase activity of human dental pulp cells (HPCs).


METHODS
HPCs were cultivated from connective tissue explants digested with collagenase type I. The expression of mCD14 was determined, and the effects of LPS on proliferation of HPCs at the concentrations of 0.1μg/ml,1μg/ml and 10μg/ml were observed by FCM. The effects of LPS tested on ALPase activity were examined. X(2) test and one-way ANOVA were used in statistical analysis respectively.


RESULTS
The results showed that LPS receptor mCD14 was not present in HPCs. LPS inhibited proliferation of HPCs (P<0.01) and ALPase activity in HPCs (P<0.05) at the concentrations of 0.1μg/ml,1μg/ml and 10μg/ml.


CONCLUSIONS
It is concluded that LPS tested inhibits proliferation of HPCs and ALPase activity of HPCs.
Wang
Feng-Ming
FM
Institute of Endodontology, West China College of Stomatology, Sichuan University. Chengdu 610041, Sichuan Province, China.
Hu
Tao
T
Zhou
Xue-Dong
XD
D000469:Alkaline Phosphatase / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D008070:Lipopolysaccharides
D008070:Lipopolysaccharides; D000469:Alkaline Phosphatase