title: Preparation of single cell detritus from Laminaria saccharina as a hatchery diet for bivalve mollusks.
abstract: A high-yield technique is described for the elaboration of single cell detritus (SCD) from Laminaria saccharina, based on the sequential action of C1H, enzymes (endoglucanases and cellulases) and 2 bacteria showing a high degree of cellobiotic, proteolytic, and alginolytic activity (CECT 5255 and CECT 5256). Over 85% of dried particles of L. saccharina were transformed into a suspension of free cell and bacterial and detrital particles after 24 hours of bacterial activity with this technique. These particles were less than 20 mum in diameter, constituting a suitable diet for bivalve mollusks. After 72 hours 99% of the total particulate volume consisted of particles less than 20 mum in diameter. Tests of hatchery diets for the seed of clam Ruditapes decussatus revealed increases of 54% and 68% for live weight and length, respectively, when SCD from L. saccharina was used as the sole dietary component compared with a live phytoplankton diet. However, SCD from L. saccharina is not a suitable food for the larvae of R. decussatus.
authors.affiliation: Instituto Español de Oceanografía, Muelle de Animas s/n, 15001 La Coruña, Spain. alejandro.perez@co.es
authors.forename: Pérez
authors.initials: P
authors.lastname: Camacho
authors.forename: J M
authors.initials: JM
authors.lastname: Salinas
authors.forename: C
authors.initials: C
authors.lastname: Fuertes
authors.forename: M
authors.initials: M
authors.lastname: Delgado
mesh_terms: D000704:Analysis of Variance; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D002480:Cellulase / Q000378:metabolism; D004032:Diet; D007795:Laminaria / Q000302:isolation & purification*; D008974:Mollusca / Q000254:growth & development*
chemical_list: D002480:Cellulase