The common insertional polymorphism in the APOC1 promoter is associated with serum apolipoprotein C-I levels in Hispanic children.
We examined the effect of APOC1-317insCGTT allele status (HpaI RFLP, deletion [H1] and insertion [H2] alleles) on serum apolipoprotein (apo) C-I level in 362 Hispanic children in the Columbia University BioMarkers Study. The H2 allele was present in 147 subjects (40.6%). Serum apoC-I was 20% lower in the presence of the H2 allele in APOE epsilon3/epsilon3 homozygotes (P=0.003) but did not differ by H2 status in epsilon4 carriers. Insufficient numbers of epsilon2 carriers (N=45) were present for analysis. In multivariate analysis in the epsilon3/epsilon3 context, after adjusting for potential covariate effects and familial aggregation, the mean effect of H2/* versus H1/H1 on apoC-I level, was estimated to be 2.15+/-0.55mg/dl (P<0.0025). Plasma triglyceride level was weakly correlated with serum apoC-I level (Pearson's r=0.17, P<0.001) but was highly correlated with serum apoC-III (Pearson's r=0.74, P<0.0001). Nevertheless, presence of the H2 allele was not significantly associated with serum apoC-III level. Thus, the effect of APOC1 genotype on serum apoC-I level was not due to apoC-I level serving as a surrogate for triglyceride level. The APOC1-317insCGTT allele is a commonly polymorphic genetic marker that is associated with serum apoC-I level in the APOE epsilon3/epsilon3 context. These findings suggest that the mechanism of the previously described association with plasma TG is, at least in part, related to the correlation of the polymorphism with the level of expression of apoC-I.
Shachter
Neil S
NS
Department of Medicine, Columbia University, 630 West 168th Street, New York, NY 10032, USA. nss5@columbia.edu
Rabinowitz
Daniel
D
Stohl
Sheldon
S
Conde-Knape
Karin
K
Cohn
Jeffrey S
JS
Deckelbaum
Richard J
RJ
Berglund
Lars
L
Shea
Steven
S
D000293:Adolescent; D053302:Apolipoprotein C-I; D001056:Apolipoproteins C / Q000097:blood* / Q000235:genetics*; D002648:Child; D003430:Cross-Sectional Studies; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006630:Hispanic or Latino; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D014280:Triglycerides / Q000097:blood*
D053302:Apolipoprotein C-I; D001056:Apolipoproteins C; D005819:Genetic Markers; D014280:Triglycerides