Multiple reporter gene assays for the assessment and estimation of chemical toxicity.
To detect chemical toxicity, we are making new bioassay systems that use promoters selected from yeast DNA microarray experiments. We performed multiple reporter gene assays using the promoters of these genes; the promoter regions were inserted upstream of green fluorescence protein (GFP). In this report, six genes (HSP26, MET17, YLL057C, FIT2, CUP1 and OYE3) were selected and assays were carried out for 55 chemicals. The promoters of these genes showed different responses to chemicals within 4 h. This result indicates that this technique enables us to predict the toxicity of chemicals in the environment and to understand toxicities of newly synthesized chemicals.
Takahashi
Junko
J
Daikin Environmental Laboratory, Ltd., 3 Banchi, Miyukigaoka, Tsukuba, Ibaraki 305-0841, Japan. junko.takahasi@daikin.co.jp
Iwahashi
Hitoshi
H
D001681:Biological Assay / Q000379:methods; D004785:Environmental Pollutants / Q000633:toxicity*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D049408:Luminescent Agents; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
D004785:Environmental Pollutants; D049408:Luminescent Agents; D049452:Green Fluorescent Proteins