title: Serotonin transporter mRNA expression in the dorsal raphe nucleus of a tumor bearing mouse.
abstract: This study was conducted to determine if an oral squamous cell carcinoma alters mRNA expression of serotonin transporter (5-HTT) in the central nervous system. KB cell line derived from a human oral squamous cell carcinoma was inoculated into nude mice, and mRNA expression level of 5-HTT in the dorsal raphe nucleus (DRN) was examined by in situ hybridization when the tumor mass reached to -10% of total body weight. Plasma leptin levels were determined by radioimmunoassay method using a commercial kit. 5-HTT mRNA level was significantly decreased in the DRN of tumor bearing mice, compared to the age-matching non-tumor control. Plasma leptin level decreased concomitantly in tumor bearing mice. These results suggest that oral carcinoma may suppress 5-HTT gene expression in the central nervous system, perhaps in relation with decreased plasma leptin level.
authors.affiliation: Department of Pharmacology, BK21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Jahng
authors.forename: Si Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Mi Joo
authors.initials: MJ
authors.lastname: Cha
authors.forename: Nam Youl
authors.initials: NY
authors.lastname: Kim
authors.forename: Soon Jeong
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001835:Body Weight; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D018076:DNA, Complementary; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020738:Leptin / Q000097:blood; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011863:Radioimmunoassay; D011903:Raphe Nuclei / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins
chemical_list: D018076:DNA, Complementary; D020738:Leptin; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse; D012701:Serotonin