[Optical properties of human normal small intestine tissue with theoretical model of optics about biological tissues at Ar+ laser and 532 nm laser and their linearly polarized laser irradiation in vitro].
A double-integrating-spheres system, basic principle of measuring technology of ray radiation, and optical model of biological tissues were used for the study. Optical properties of human normal small intestine tissue at 476.5, 488, 496.5, 514.5 and 532 nm laser and their linearly polarized laser irradiation were studied. The results of measurement showed that the total attenuation coefficient and scattering coefficient of the tissue at these wavelengths of laser and their linearly polarized laser irradiation increased with decreasing wavelengths. And obviously there was a distinction at 514.5 to 532 nm wavelength between lasers and their linearly polarized laser irradiation. Absorption coefficient of tissue at these wavelengths of laser and their linearly polarized laser irradiation increased with decreasing wavelengths. Absorption coefficient of tissue at 514.5 to 532 nm wavelength of laser was obviously decreasing, which was independent of these wavelengths of laser or their linearly polarized laser irradiation. Mean cosine of scattering of tissue at these wavelengths of laser and their linearly polarized laser irradiation also increased with decreasing wavelengths. But penetration depth of tissue at these wavelengths of laser and their linearly polarized laser irradiation also increased with increasing of wavelengths. Refractive index of tissue between these wavelengths of laser was within 1.38 to 1.48. Absorption coefficient, scattering coefficient, total attenuation coefficient, effective attenuation coefficients of tissue in Kubelka-Munk two-flux model at the same wavelength of laser and their linearly polarized laser irradiation showed no prominent distinction (P>0.01). Absorption coefficient, scattering coefficient, total attenuation coefficient, effective attenuation coefficients of tissue in Kubelka-Munk two-flux model at different wavelength of laser and their linearly polarized laser irradiation showed obvious distinction. Optical properties of tissue at 514.5 to 532 nm wavelength of laser exhibited obvious distinction.
Wei
Hua-jiang
HJ
Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China.
Xing
Da
D
Wu
Guo-yong
GY
Jin
Ying
Y
Gu
Huai-min
HM
D000042:Absorption; D000465:Algorithms; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000166:cytology; D017075:Laser Coagulation; D007834:Lasers; D008027:Light; D008954:Models, Biological; D009010:Monte Carlo Method; D055095:Optics and Photonics / Q000379:methods; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation