The fabrication of a colloidal gold-carbon nanotubes composite film on a gold electrode and its application for the determination of cytochrome c.
Colloid Au (Au(nano)) with a diameter of about 20 nm was prepared and used in combination with the multi-wall carbon nanotubes (MWNTs) to modify a gold electrode. Dihexadecylphosphate (DHP) dispersed in Au(nano) aqueous solution was used to solubilize MWNTs. Deposition of Au(nano) on MWNTs was realized as illustrated by TEM micrographs. The DHP formed a network that connected Au(nano) and MWNTs to the gold electrode surface. The Au(nano)-MWNTs-DHP composite film on the gold electrode surface was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammmetry (CV). The composite film modified gold electrode was used to detect cytochrome c and a pair of well-defined redox waves was obtained. It was found that the composite film promoted the redox of horse heart cytochrome c and its effect was developed for the determination of cytochrome c.
Wu
Yunhua
Y
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Hu
Shengshui
S
D015374:Biosensing Techniques; D045304:Cytochromes c / Q000032:analysis*; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D017979:Gold Colloid / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010755:Organophosphates / Q000737:chemistry; D011199:Potentiometry
D017979:Gold Colloid; D008081:Liposomes; D037742:Nanotubes, Carbon; D010755:Organophosphates; C002290:dicetylphosphate; D045304:Cytochromes c