title: [Viability of the spleen in rats after ligation of the splenic vessels: effects of hyperbaric oxygen therapy].
abstract: OBJECTIVE
  To investigate the effects of splenic artery and vein ligation and the influence of hyperbaric oxygen after the double vascular ligation on the viability of spleen tissue.
  
  
  METHODS
  Sixty nine adult male Wistar rats (285-375 g) were randomly separated in three groups: group 1, four rats, sham operated, group 2, 34 rats, submitted to simultaneous splenic artery and vein ligation and group 3, 31 rats, submitted to hyperbaric oxygen during 11 days, after double vascular ligation. All animals were killed on day 12 after surgery. The spleen was removed and paraffin embedded for microscopic examination.
  
  
  RESULTS
  In the groups submitted to vascular ligation, the spleen was normal in 8.82% of rats not treated with hyperbaric oxygen and in 45.16% of rats that received hyperbaric oxygen after vascular ligation (p=0.01). In the spleens with white infarct, the mass of preserved splenic tissue in relation to the total body mass did not differ between the groups treated or not with hyperbaric oxygen. The preserved splenic tissue had normal histology in both groups. The healing process was more accelerated in the group of rats treated with hyperbaric oxygen.
  
  
  CONCLUSIONS
  Results demonstrate that exposure to hyperbaric oxygen increased the frequency of total spleen mass preservation after simultaneous ligation of the splenic artery and vein but did not alter the percentage of the spleen's viable area, however the healing process in necrotic areas was accelerated.
authors.affiliation: Laboratório de Técnica Operatória e Cururgia Experimental da Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória, ES.
authors.forename: Danilo Nagib Salomão
authors.initials: DN
authors.lastname: Paulo
authors.forename: Mitre
authors.initials: M
authors.lastname: Kalil
authors.forename: Luiz Sérgio Pereira
authors.initials: LS
authors.lastname: Grillo Junior
authors.forename: Erick Barcelos
authors.initials: EB
authors.lastname: Borges
authors.forename: Luiz Cálice
authors.initials: LC
authors.lastname: Cintra
authors.forename: Fausto Edmundo Lima
authors.initials: FE
authors.lastname: Pereira
authors.forename: Alcino Lázaro
authors.initials: AL
authors.lastname: da Silva
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation; D008026:Ligation / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000098:blood supply* / Q000473:pathology; D013157:Splenic Artery; D013159:Splenic Infarction / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D013162:Splenic Vein