Preparation of a gradient biotinylated polyethylene surface to bind streptavidin-FITC.
A novel biotinylated gradient surface was designed to bind a streptavidin-FITC. The gradient polyethylene (PE) surface was prepared by a corona treatment with a power increase along the PE length, and successively, biotinylated gradient PE surface was prepared by the reaction of polyethylenimine (PEI) and biotin. Surface properties of the gradient PE surface changed according to PE length and chemical modification. The biotinylated gradient PE surface provided a continuous gradient of streptavidin-FITC through binding of avidin-biotin. The luminosity of fluorescence that corresponds to a streptavidin-bound FITC on the biotinylated gradient PE surface increased gradually on the surface along the PE length.
Kim
Moon Suk
MS
Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Korea.
Seo
Kwang Su
KS
Khang
Gilson
G
Lee
Hai Bang
HB
D019921:Biotinylation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D005453:Fluorescence; D020959:Polyethylene / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties
D020959:Polyethylene; D019809:Streptavidin; D016650:Fluorescein-5-isothiocyanate