title: Variety of TEM-, SHV-, and CTX-M-type beta-lactamases present in recent clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae from Taiwan.
abstract: A total of 171 hospitals' isolates of E. coli, K. pneumoniae, and E. cloacae with a minimum inhibitory concentration (MIC) of > or =2 microg/ml for ceftazidime or cefotaxime were evaluated for the production of beta-lactamases. PCR amplification with specific primers for the bla (SHV), bla (TEM), and bla (CTX) genes revealed that a total of 53, 81, and 43 of these genes were amplified, respectively. Sequencing results confirmed that TEM-1, CTX-M-3 and -14, SHV-1, -5, -11, -12, and -33, OXY-1a, and LEN-1 were presented among these isolates. No specific large cluster of isolates carried the same beta-lactamases, indicating the wide diversity of the collected strains. Plasmid spread between E. coli and K. pneumoniae was identified in few isolates. Combinations of TEM, SHV, and CTX beta-lactamase genes, including extended-spectrum beta-lactamase genes, were observed in all three species.
authors.affiliation: Division of Clinical Research, National Health Research Institute, Taipei 11529, Taiwan.
authors.forename: Ling
authors.initials: L
authors.lastname: Ma
authors.forename: Feng-Yee
authors.initials: FY
authors.lastname: Chang
authors.forename: Chang-Phone
authors.initials: CP
authors.lastname: Fung
authors.forename: Te-Li
authors.initials: TL
authors.lastname: Chen
authors.forename: Jung-Chung
authors.initials: JC
authors.lastname: Lin
authors.forename: Po-Liang
authors.initials: PL
authors.lastname: Lu
authors.forename: Li-Yueh
authors.initials: LY
authors.lastname: Huang
authors.forename: Jen-Chang
authors.initials: JC
authors.lastname: Chang
authors.forename: L K
authors.initials: LK
authors.lastname: Siu
mesh_terms: D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D013624:Taiwan; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000302:isolation & purification*
chemical_list: C425114:beta-lactamase CTX-M-10; C062760:beta-lactamase SHV-2; C061036:beta-lactamase TEM-4; D001618:beta-Lactamases