Spontaneously active and InsP3-activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurones.
Increases in Ca(2+) concentration in the nucleus of neurones modulate gene transcription and may be involved in activity-dependent long-term plasticity, apoptosis, and neurotoxicity. Little is currently known about the regulation of Ca(2+) in the nuclei of neurones. Investigation of neuronal nuclei is hampered by the cellular heterogeneity of the brain where neurones comprise no more than 10% of the cells. The situation is further complicated by large differences in properties of different neurones. Here we report a method for isolating nuclei from identified central neurones. We employed this technique to study nuclei from rat cerebellar Purkinje and granule neurones. Patch-clamp recording from the nuclear membrane of Purkinje neurones revealed numerous large-conductance channels selective for monovalent cations. The nuclear membrane of Purkinje neurones also contained multiple InsP(3)- activated ion channels localized exclusively in the inner nuclear membrane with their receptor loci facing the nucleoplasm. In contrast, the nuclear membrane of granule neurones contained only a small number of mainly anion channels. Nuclear InsP(3) receptors (InsP(3)Rs) were activated by InsP(3) with EC(50) = 0.67 microm and a Hill coefficient of 2.5. Ca(2+) exhibited a biphasic effect on the receptors elevating its activity at low concentrations and inhibiting it at micromolar concentrations. InsP(3) in saturating concentrations did not prevent the inhibitory effect of Ca(2+), but strongly increased InsP(3)R activity at resting Ca(2+) concentrations. These data are the first evidence for the presence of intranuclear sources of Ca(2+) in neurones. Ca(2+) release from the nuclear envelope may amplify Ca(2+) transients penetrating the nucleus from the cytoplasm or generate Ca(2+) transients in the nucleus independently of the cytoplasm.
Marchenko
Sergey M
SM
Bogomoletz Institute of Physiology, 4 Bogomoletz Street, Kiev, 01024, Ukraine. smm@serv.biph.kiev.ua
Yarotskyy
Victor V
VV
Kovalenko
Tatiana N
TN
Kostyuk
Platon G
PG
Thomas
Roger C
RC
D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009685:Nuclear Envelope / Q000502:physiology* / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D002118:Calcium