Production of transgenetic sugarbeet (Beta vulgaris L.) plants resistant to phosphinothricin.
A method of Agrobacterium-mediated genetic transformation of sugarbeet (Beta vulgaris L.) with vacuum infiltration has been developed. Aseptic 3-weeks old etiolated seedlings of two diploid O-type sugarbeet lines (KS3 and KS7) have been used for genetic transformation. Transgenic sugarbeet plants carrying the reporter beta-glucuronidase gene have been selected for their resistance to glufosinate ammonium herbicide. Integration of transgenes into sugarbeet genome was confirmed with GUS assay and PCR using primers for bar and gusA genes.
Kishchenko
E M
EM
Institute of Cell Biology & Genetic Engineering Natl. Acad. Sci. of Ukraine, 148 Zabolotnogo Str., Kyiv 03143, Ukraine. iicb@iicb.kiev.ua
Komarnitskii
I K
IK
Kuchuk
N V
NV
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000613:Aminobutyrates / Q000494:pharmacology*; D027461:Beta vulgaris / Q000187:drug effects / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000378:metabolism; D012038:Regeneration; D014170:Transformation, Genetic
D000613:Aminobutyrates; D006540:Herbicides; C003121:phosphinothricin; D005966:Glucuronidase