title: Congenital diaphragmatic hernia, tracheal occlusion, thyroid transcription factor-1, and fetal pulmonary epithelial maturation.
abstract: Congenital diaphragmatic hernia (CDH) occurs in approximately 1:2,500 human births and has high morbidity and mortality rates, primarily due to pulmonary hypoplasia and pulmonary hypertension. Tracheal occlusion (TO), in experimental animals, distends lungs and increases lung growth and alveolar type I cell maturation but decreases surfactant components and reduces alveolar type II cell density. We examined effects of CDH and CDH+TO on lung growth and maturation in fetal rats. To induce CDH, we administered nitrofen (100 mg) to dams at 9.5 days of gestation. We compared lungs from fetuses with CDH, CDH+TO, and those exposed to nitrofen without CDH. CDH decreased lung wet weight bilaterally (P < 0.0001) and DNA content in lung ipsilateral to CDH (P < 0.05). CDH+TO significantly increased lung wet weights bilaterally; DNA content was intermediate between CDH and NC. To evaluate effects on the distal pulmonary epithelium, we examined surfactant mRNA and protein levels, type I and II cell-specific markers (RTI(40) and RTII(70), respectively), and transcriptional regulator thyroid transcription factor-1 (TTF-1). Decreased lung distension (due to CDH) increased SP-C mRNA and TTF-1 protein expression and reduced RTI(40) (P < 0.05 for all). Increased lung distension (due to CDH+TO) reduced expression of SP mRNAs and pro-SP-C and TTF-1 proteins and enhanced expression of RTI(40) (mRNA and protein; P < 0.05 for all). We conclude that CDH+TO partially reverses effects of CDH; it corrects the pulmonary hypoplasia and restores type I cell differentiation but adversely affects SP expression in type II cells. These effects may be mediated through changes in TTF-1 expression.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, 94143, USA. cheri@itsa.ucsf.edu
authors.forename: Cheryl J
authors.initials: CJ
authors.lastname: Chapin
authors.forename: Robert
authors.initials: R
authors.lastname: Ertsey
authors.forename: Jyoji
authors.initials: J
authors.lastname: Yoshizawa
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hara
authors.forename: Lourenco
authors.initials: L
authors.lastname: Sbragia
authors.forename: John J
authors.initials: JJ
authors.lastname: Greer
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Kitterman
mesh_terms: D000402:Airway Obstruction / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005325:Fetal Organ Maturity / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006548:Hernia, Diaphragmatic / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000196:embryology* / Q000473:pathology; D008297:Male; D009687:Nuclear Proteins / Q000096:biosynthesis*; D010575:Pesticides / Q000633:toxicity*; D010647:Phenyl Ethers / Q000633:toxicity*; D011247:Pregnancy; D011270:Pregnancy, Animal; D037721:Pulmonary Surfactant-Associated Protein C / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D000074482:Thyroid Nuclear Factor 1; D014132:Trachea / Q000196:embryology / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: C000614412:NKX2-1 protein, human; C000614416:Nkx2-1 protein, rat; D009687:Nuclear Proteins; D010575:Pesticides; D010647:Phenyl Ethers; D037721:Pulmonary Surfactant-Associated Protein C; D012333:RNA, Messenger; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; C007350:nitrofen