[Study on the reaction of Lys-ZCN-CTMAB complex].
The interaction of lysozyme (Lys), O-{2-[alpha-(2-Hydroxy-5-Sulphophenlylazo)-benzylidene] hydrazino} benzoic acid (ZCN) and cetyltrimethy ammonicumbromide (CTMAB) was investigated by UV spectrophotometric method. The effect of temperature, time, and ion intensity in the buffer solution (pH 4.46) on reaction system was determined. Linear range of the reaction was 0-10 microg x mL(-1). The molar absorptivity was epsilon = 4.6164 x 10(5) L x cm(-1) x mol(-1). The reaction mechanism and interference of inorganic substance, biological substance and surface active agent to the reaction was investigated. Study system was established.
Bai
Jin-wei
JW
College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621002, China.
Chi
Yan-hua
YH
Zhuang
Jia
J
Li
Ke-an
KA