title: Fluorescence in situ hybridization and spectral imaging analysis of human oocytes and first polar bodies.
abstract: We investigated the frequencies of abnormalities involving either chromosome 1, 16, 18, or 21 in failed-fertilized human oocytes. Although abnormalities involving chromosome 16 showed an age-dependent increase, results for the other chromosomes did not show statistically significant differences among the three age groups, <35 years, 35-39 years, and >39 years. The scoring of four chromosomes is likely to underestimate the true rate of aneuploid cells. Therefore, for a pilot study investigating a more-comprehensive analysis of oocytes and their corresponding first polar bodies, we developed a novel eight-probe chromosome enumeration scheme using fluorescence in situ hybridization and spectral imaging analysis.
authors.affiliation: Life Sciences Division, MS 74-157, E.O. Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA. ulliweier@hotmail.com
authors.forename: Heinz-Ulli G
authors.initials: HU
authors.lastname: Weier
authors.forename: Jingly F
authors.initials: JF
authors.lastname: Weier
authors.forename: Maria Oter
authors.initials: MO
authors.lastname: Renom
authors.forename: Xuezhong
authors.initials: X
authors.lastname: Zheng
authors.forename: Pere
authors.initials: P
authors.lastname: Colls
authors.forename: Aida
authors.initials: A
authors.lastname: Nureddin
authors.forename: Chau D
authors.initials: CD
authors.lastname: Pham
authors.forename: Lisa W
authors.initials: LW
authors.lastname: Chu
authors.forename: Catherine
authors.initials: C
authors.lastname: Racowsky
authors.forename: Santiago
authors.initials: S
authors.lastname: Munné
mesh_terms: D000328:Adult; D000367:Age Factors; D000782:Aneuploidy; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D009865:Oocytes / Q000166:cytology* / Q000648:ultrastructure