title: [Comparative analysis of the major protective antigens production in Vibrio cholerae recombinant and producer strains of the classical biovar].
abstract: The comparative study of 4 constructed protective antigen producing strains of the classical biovar and V. cholerae strains 569 B Inaba and M41 Ogawa, used in manufacturing the cholera chemical vaccine "cholerogen-toxoid", was carried out. The study revealed that V. cholerae plasmid strains 2414 Ogawa, 2415 Inaba and nonplasmid strains 2416 Ogawa, 2417 Inaba had a higher level of production of the main protective antrigens in comparison with producer strains. They also synthesized much more (4-5 fold) cholera toxin, toxin co-regulated adhesion pili, contained protein OmpU in their outer membrane, exceeded 2- to 3-fold in the synthesis of pathogenicity enzymes (proteases, phospholipases) and synthesized the same amounts of 01 antigen, serovars Inaba and Ogawa. The use of the newly created protective-antigen producing strains in vaccine manufacturing could facilitate the preparation of a more effective cholera chemical vaccine "cholerogen-toxoid".
authors.forename: A V
authors.initials: AV
authors.lastname: Toporkov
authors.forename: S P
authors.initials: SP
authors.lastname: Zadnova
authors.forename: N I
authors.initials: NI
authors.lastname: Smirnova
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000096:biosynthesis*; D001422:Bacterial Adhesion / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D002772:Cholera Toxin / Q000096:biosynthesis; D022121:Cholera Vaccines / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000276:immunology; D010447:Peptide Hydrolases / Q000096:biosynthesis; D010740:Phospholipases / Q000096:biosynthesis; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D014121:Toxoids / Q000096:biosynthesis; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology; D014734:Vibrio cholerae / Q000201:enzymology / Q000235:genetics / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D022121:Cholera Vaccines; C096349:OmpU protein, Vibrio cholerae; D011994:Recombinant Proteins; D014121:Toxoids; D014614:Vaccines, Synthetic; C038458:cholera toxoid; D002772:Cholera Toxin; D010740:Phospholipases; D010447:Peptide Hydrolases