title: Detection of immune-complexed 9-O-acetylated sialoglycoconjugates in the sera of patients with pediatric acute lymphoblastic leukemia.
abstract: Although childhood acute lymphoblastic leukemia (ALL) is highly responsive to chemotherapy, reliable techniques are needed to determine treatment outcome. Over expression of 9-O-acetylated sialoglycoconjugates (9-OAcSGs) on lymphoblasts and concomitant anti-9-OAcSGs was found to have a diagnostic and prognostic potential. However, the presence of circulatory immune-complexed antigens remains unknown. The present study was aimed to evaluate whether immune-complexed 9-OAcSGs can be harnessed for better disease management. Immune-complexed antigens were evaluated in ALL sera (n=262) by a Dot-blot using a 9-OAcSAalpha2-6GalNAc-specific lectin, Achatinin-H. Using three serum samples, the inter- and intra-assay imprecision was evaluated as 11-13% and 7-11%, respectively. The recovery of spiked 9-OAcSGs was 84.2-95.4%. The central 95% reference interval for immune-complexed 9-OAcSGs in normal human sera (NHS, n=144) was 2.9-3.4 mug/ml irrespective of sex and age. At disease presentation, the immune-complexed 9-OAcSGs were fivefold higher than NHS, decreased with remission induction and importantly, reappeared with clinical relapse. Sera from patients with other hematological disorders (n=86) showed negligible levels. The Dot-blot demonstrated the potential application of immune-complexed antigen as a disease-specific marker and its efficacy as a sensitive and specific method that could serve as an economical yet effective index for monitoring disease status.
authors.affiliation: Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata-700 032, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: Kankana
authors.initials: K
authors.lastname: Mukherjee
authors.forename: Mitali
authors.initials: M
authors.lastname: Chatterjee
authors.forename: Dilip Kumar
authors.initials: DK
authors.lastname: Bhattacharya
authors.forename: Chitra
authors.initials: C
authors.lastname: Mandal
mesh_terms: D000293:Adolescent; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006001:Glycoconjugates / Q000097:blood*; D000949:Histocompatibility Antigens Class II / Q000097:blood; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D037102:Lectins / Q000097:blood / Q000276:immunology; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis*; D011379:Prognosis; D012794:Sialic Acids / Q000097:blood*; D016896:Treatment Outcome
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D006001:Glycoconjugates; D000949:Histocompatibility Antigens Class II; D037102:Lectins; D012794:Sialic Acids; C050704:achatinin(H)