title: Phylogenetic relationships of methionine aminopeptidase 2 among Encephalitozoon species and genotypes of microsporidia.
abstract: This report describes the characterization and phylogenetic analysis of the deduced amino acid sequences of methionine aminopeptidase 2 (MetAP-2) enzymes from microsporidian species and genotypes of the genus Encephalitozoon. Fragments of DNA encoding 318 to 335 amino acid residues of the MetAP-2 genes were isolated from genomic DNA prepared from cultured spores of Encephalitozoon hellem, Encephalitozoon intestinalis, and Encephalitozoon cuniculi genotypes I-III. Sequence comparisons of the deduced amino acid residues indicated that the microsporidian sequences are MetAP-2-like rather than MetAP-1-like. Alignments demonstrated that the new Encephalitozoon sequences included sequences and structures conserved in eukaryotic MetAP-2s, including the five conserved, active site residues, Asp, Asp, His, Glu, and His, considered to be critical for catalysis and for coordinating the cation (e.g., cobalt) co-factor, and included residues known to interact with the antibiotic, fumagillin. The primary structure of the Encephalitozoon MetAP-2s, however, showed some dissimilarity with human and yeast MetAP-2s, including the absence of the NH2-terminal polylysine tract. Phylogenetic comparison of these Encephalitozoon MetAP-2s with orthologues from related species and from other informative taxa confirmed that the MetAP-2s of these Encephalitozoon species and strains are closely related to each other and cluster with MetAP-2s.
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA.
authors.forename: Ivona
authors.initials: I
authors.lastname: Pandrea
authors.forename: Derek
authors.initials: D
authors.lastname: Mittleider
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Brindley
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Didier
authors.forename: David L
authors.initials: DL
authors.lastname: Robertson
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D017124:Conserved Sequence; D003510:Cyclohexanes; D016819:Encephalitozoon / Q000235:genetics*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005838:Genotype; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012717:Sesquiterpenes; D033761:Spores, Protozoan / Q000235:genetics
chemical_list: D003510:Cyclohexanes; D005231:Fatty Acids, Unsaturated; D012717:Sesquiterpenes; C026211:fumagillin; D000626:Aminopeptidases; C106541:methionine aminopeptidase 2; D008666:Metalloendopeptidases