[Effect of adenosine A1-receptors on the respiratory rhythmical discharge activity in neonatal rat brainstem slice in vitro].
OBJECTIVE
To explore the role of adenosine A(1)-receptors in the generation and regulation of central respiratory rhythm.


METHODS
Neonatal rat brainstem slices including the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve rootlets retained were prepared in which the respiratory activity was recorded from the hypoglossal nerve rootlets by suction electrode. The possible role of adenosine A(1)-receptors on rhythmical respiration was investigated by administration of specific antagonist of adenosine A(1)-receptor R-phenylisopropyl-adenosine (R-PIA) and its antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) in modified Kreb's solution (MKS) for perfusion of the brainstem slices.


RESULTS
DPCPX induced a significant decrease in the expiratory time and respiratory cycles. However, R-PIA significantly prolonged the respiratory cycle and decreased the inspiratory time and the integral amplitude. The effect of R-PIA on the rhythmical discharges could be partially reversed by additional application of DPCPX.


CONCLUSIONS
Adenosine A(1)-receptors are involved in the modulation of rhythmical respiratory in neonate rat brainstem.
Wang
Jian-Li
JL
Department of Physiology, Southern Medical University, Guangzhou 510515, China. icestove79@163.com
Wu
Zhong-Hai
ZH
D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000502:physiology*; D004566:Electrodes; D004594:Electrophysiology; D066298:In Vitro Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D043682:Receptor, Adenosine A1 / Q000502:physiology*; D012119:Respiration; D012125:Respiratory Center / Q000502:physiology
D043682:Receptor, Adenosine A1