An improved formulation of the zirconyl hematoxylin stain for acidic mucins.
Zirconyl hematoxylin stains acidic mucins darkly and specifically using a solution of 100 mg hematoxylin, 5 ml ethanol, 5 ml 0.5% sodium iodate, 400 mg zirconyl chloride octahydrate, and 30 ml 25% aqueous glycerol. The stain is especially advantageous for studying goblet cells and Paget cells.
McNulty
J M
JM
Barry University School of Graduate Medical Sciences, Miami Shores, Florida 33161, USA.
Smith
A A
AA
D000818:Animals; D004396:Coloring Agents / Q000737:chemistry; D020397:Goblet Cells / Q000166:cytology*; D006416:Hematoxylin / Q000031:analogs & derivatives* / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009077:Mucins / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D012756:Sheep; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; D009077:Mucins; C418048:zirconyl hematoxylin; D006416:Hematoxylin