title: Structural characterization and transcriptional regulation of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide in the cotton bollworm, Helicoverpa armigera.
abstract: We have cloned the gene encoding the diapause hormone and the pheromone biosynthesis activating neuropeptide in Helicoverpa armigera (Har-DH-PBAN). The Har-DH-PBAN gene contains six exons and five introns that fall in the same positions as in the Bombyx mori DH-PBAN gene (Bom-DH-PBAN). The transcription initiation site lays 29 bp upstream of the translation initiation site. Southern blot analysis suggests that a single copy of this gene is present per haploid genome. A structural comparison of DH-PBAN promoters between H. armigera and B. mori show similarities in the TATA box and in a potential binding site for a POU family transcription factor, POU-M2. However, testing of these DNA regions for factor binding in vitro and transcription assays in cell culture highlight significant differences in their regulation particularly in reference to the POU-M2 sites. Our results uncover common and different regulatory mechanisms at work in the control of DH-PBAN gene expression in H. armigera and B. mori.
authors.affiliation: Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Zhang
authors.forename: Jiu-Song
authors.initials: JS
authors.lastname: Sun
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Liu
authors.forename: Le
authors.initials: L
authors.lastname: Kang
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Shen
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D024202:Electrophoretic Mobility Shift Assay; D040461:Gene Components; D005786:Gene Expression Regulation; D015698:Genomic Library; D008156:Luciferases; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D009479:Neuropeptides; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea; C075027:diapause hormone; D008156:Luciferases