[Effects of low shear stress on expression of platelet derived growth factor (PDGF) of organ-cultured artery in vitro].
To explore the biological effects of shear stress on intact artery and the change of growth factor during stress-induced vascular remodeling, we established an artery organ-cultured system under stress in vitro, and the common carotid arteries of pigs were cultured under shear stress of 20, 5 and 0 dyn/cm2. PDGF-A synthesis of vascular smooth muscle cells (VSMCs) cultured for 1, 4 and 7 days were studied by immunohistochemical and computer image processing methods, and PDGF-B secretion of endothelial cells (ECs) cultured within 12 h were studied by ELISA. Results showed that PDGF-B increased obviously under shear stress of 5 dyn/cm2, and reached the highest point at about 3 h; PDGF-A synthesis also obviously increased under low shear stress in 7 days. Increasing of PDGF synthesis promotes phenotype switch and proliferation of VSMC. It may have important influence on artery remodeling under low shear stress.
Liu
Bo
B
Laboratory of Biomechanics, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
Zhou
Jin
J
Jiang
Zonglai
Z
Zhang
Yan
Y
Liu
Yanchun
Y
Yan
Zhiqiang
Z
Li
Yuquan
Y
D000818:Animals; D017536:Carotid Artery, Common / Q000737:chemistry* / Q000378:metabolism*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D033081:Shear Strength; D013314:Stress, Mechanical; D013552:Swine
D010982:Platelet-Derived Growth Factor