title: Molecular diagnosis of Fusobacterium necrophorum infection (Lemierre's syndrome).
abstract: Presented here is the case of a 27-year-old male with atypical features of Lemierre's syndrome in which a definitive diagnosis was achieved using molecular methods. While routine investigations, including bacterial cultures, were unhelpful, two real-time PCR assays demonstrated Fusobacterium necrophorum-specific DNA in aspirates from brain and renal abscesses. This is the first report demonstrating that a laboratory diagnosis can be made using molecular methods in suspected cases of Lemierre's syndrome. Use of these methods can thus resolve diagnostic confusion, prevent unnecessary investigation, and direct specific antimicrobial treatment.
authors.affiliation: Health Protection Agency, Clinical Microbiology and Public Health Laboratory, Box 236, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 2QW, UK. sani.aliyu@addenbrookes.nhs.uk
authors.forename: S H
authors.initials: SH
authors.lastname: Aliyu
authors.forename: P F K
authors.initials: PF
authors.lastname: Yong
authors.forename: M J
authors.initials: MJ
authors.lastname: Newport
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: R K
authors.initials: RK
authors.lastname: Marriott
authors.forename: M D
authors.initials: MD
authors.lastname: Curran
authors.forename: H
authors.initials: H
authors.lastname: Ludlam
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis; D005674:Fusobacterium Infections / Q000097:blood / Q000175:diagnosis*; D005675:Fusobacterium necrophorum / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D013577:Syndrome
chemical_list: D004269:DNA, Bacterial