Evaluation of the EVIGENE VRE detection kit for detection of vanA and vanB genes in vancomycin-resistant enterococci.
The aim of this study was to evaluate the performance of the EVIGENE VRE Detection kit and compare it with PCR, considered the gold standard for detection of vancomycin-resistant enterococci (VRE). The correlation between the MIC values of vancomycin and teicoplanin using the epsilon test was also determined. In the EVIGENE VRE Detection kit, DNA probes specific for bacterial target DNA sequences are bound to microwell plates. A hundred and ten VRE (104 Enterococcus faecium and six Enterococcus faecalis) and 45 vancomycin-susceptible E. faecium were tested. All VRE strains were found to be positive for the vanA genotype using the EVIGENE VRE Detection kit. All results obtained with the EVIGENE VRE Detection kit were confirmed by PCR. MIC results for the strains also correlated highly with the PCR and kit results. The EVIGENE VRE Detection kit should be used in preference to other methods for detecting resistance genes in all strains, since it is less time-consuming, does not require the handling of hazardous chemicals and has the same specificity as PCR.
Kilic
Abdullah
A
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Baysallar
Mehmet
M
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Bahar
Gul
G
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Kucukkaraaslan
Ayten
A
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Cilli
Feriha
F
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
Doganci
Levent
L
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D020713:Vancomycin Resistance
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases