title: Reproducibility of gemifloxacin and comparison fluoroquinolone MIC results using Sensititre commercial dry-form panels.
abstract: This study examined the ability of commercial dry-form broth microdilution panels (Sensititre, TREK Diagnostics, Cleveland, OH) to produce accurate minimum inhibitory concentration (MIC) results for gemifloxacin, a new fluoroquinolone. Reproducibility for gemifloxacin was 100.0% for on-scale values within +/-1 log2 dilution for same- and between-day comparisons. Newer comparator fluoroquinolones (sparfloxacin, sitafloxacin, and moxifloxacin) also showed excellent reproducibility (100.0%) for on-scale results analyzed at +/-1 log2 dilution for same- and between-day comparisons. Newer and investigational fluoroquinolone agents, including gemifloxacin, can be tested with confidence using these dry-form MIC panels.
authors.affiliation: The JONES Group/JMI Laboratories, North Liberty, IA 52317, USA. jim-ross@jmilabs.com
authors.forename: James E
authors.initials: JE
authors.lastname: Ross
authors.forename: Helio S
authors.initials: HS
authors.lastname: Sader
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Fritsche
authors.forename: Ronald N
authors.initials: RN
authors.lastname: Jones
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D000077735:Gemifloxacin; D008826:Microbial Sensitivity Tests / Q000295:instrumentation*; D009287:Naphthyridines / Q000494:pharmacology*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D009287:Naphthyridines; D011933:Reagent Kits, Diagnostic; D000077735:Gemifloxacin