[Genetic association of apoE and apoCI gene polymorphisms with coronary heart disease].
OBJECTIVE
To study the genetic association of apolipoprotein (apo) E and apoCI gene polymorphisms with coronary heart disease (CHD) in China.


METHODS
apoE genotypes were identified by multiplex amplification refractory mutation system (multi-ARMS) and the apoCI promoter polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 186 cases with CHD (age: 65.0 +/- 10.5 years) and 350 controls (age: 63.6 +/- 8.3 years). The haplotype frequencies were estimated.


RESULTS
The frequencies of apoE E4/3 genotype (26.9%) and epsilon4 (14.5%) in CHD group were significantly higher than that in the control group (12.6%, 7.0%), P <0.05. The significant difference was also found for the apoCI locus and the CHD group showed higher rate of both for the H2 allele and genotypes, carrying this allele. Estimation of the haplotype frequencies indicated that the association between the apoE-CI haplotype and CHD was significantly strong. The apoE-epsilon4/apoCI-H2 was estimated to be responsible for 9.86% of CHD.


CONCLUSIONS
When the subjects carrying both epsilon4 and H2 alleles, they would have higher risk of suffering from CHD than controls.
Wang
Chun-Hong
CH
School of public Health, Wuhan University, Wuhan 430071, China.
Zhou
Xin
X
Zhou
Guang-di
GD
Tan
Xiao-dong
XD
Han
Ding-fen
DF
Zheng
Fang
F
Liu
Fang
F
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001056:Apolipoproteins C / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D002681:China / Q000453:epidemiology; D003327:Coronary Disease / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
D001056:Apolipoproteins C; D001057:Apolipoproteins E