Structural characterization and transcriptional regulation of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide in the cotton bollworm, Helicoverpa armigera.
We have cloned the gene encoding the diapause hormone and the pheromone biosynthesis activating neuropeptide in Helicoverpa armigera (Har-DH-PBAN). The Har-DH-PBAN gene contains six exons and five introns that fall in the same positions as in the Bombyx mori DH-PBAN gene (Bom-DH-PBAN). The transcription initiation site lays 29 bp upstream of the translation initiation site. Southern blot analysis suggests that a single copy of this gene is present per haploid genome. A structural comparison of DH-PBAN promoters between H. armigera and B. mori show similarities in the TATA box and in a potential binding site for a POU family transcription factor, POU-M2. However, testing of these DNA regions for factor binding in vitro and transcription assays in cell culture highlight significant differences in their regulation particularly in reference to the POU-M2 sites. Our results uncover common and different regulatory mechanisms at work in the control of DH-PBAN gene expression in H. armigera and B. mori.
Zhang
Tian-Yi
TY
Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Sun
Jiu-Song
JS
Liu
Wen-Yu
WY
Kang
Le
L
Shen
Jin-Liang
JL
Xu
Wei-Hua
WH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D024202:Electrophoretic Mobility Shift Assay; D040461:Gene Components; D005786:Gene Expression Regulation; D015698:Genomic Library; D008156:Luciferases; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D009479:Neuropeptides; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea; C075027:diapause hormone; D008156:Luciferases