title: Development of immunoglobulin M capture enzyme-linked immunosorbent assay to differentiate human flavivirus infections occurring in Australia.
abstract: We report the development of a flavivirus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MAC-ELISA) which improves the determination of an infecting flavivirus serotype over that by current serological methods. A panel of 165 IgM-positive sera from flavivirus patients with specific diagnostic results was tested by the flavivirus MAC-ELISA using a panel of 10 antigens. For 134 of these sera (81.2%), the highest reactivity was demonstrated against the infecting virus, which was consistent with the original diagnostic result. Specific antibody reactions inconsistent with the original diagnosis were found for six sera (3.6%). In our experience, the flavivirus-serotyping ELISA provides a rapid and accurate alternative to other serological tests, such as hemagglutination inhibition, for the specific diagnosis of flavivirus infections.
authors.affiliation: Public Health Virology Laboratory, Queensland Health Scientific Services, Cooper's Plains, Australia. Carmel_Taylor@health.qld.gov.au
authors.forename: Carmel
authors.initials: C
authors.lastname: Taylor
authors.forename: Russell
authors.initials: R
authors.lastname: Simmons
authors.forename: Ina
authors.initials: I
authors.lastname: Smith
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D001315:Australia; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018178:Flaviviridae Infections / Q000175:diagnosis*; D005416:Flavivirus / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007075:Immunoglobulin M; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M