[Fluorescence fluctuation spectroscopy with strong background fluorescence--a Monte Carlo approach].
Fluorescence fluctuation spectroscopy is a method in which fluorescence fluctuations arising from a very small sample volume are analysed to obtain brightness, diffusion coefficient and concentration of particles. Using Monte Carlo simulation, the influences of background autofluorescence and noises on fluorescence fluctuation spectroscopy are studied. Results show that a two-component photon counting histogram can effectively remove the effects due to background autofluorescence from low brightness and high concentration components and uniform noise. The result will help to bring applications of fluorescence fluctuation spectroscopy to intracellular environment.
Liu
Guang
G
Key Laboratory for Atomic and Molecular NanoSciences of Education Ministry, Department of Physics, Tsinghua University, Beijing 100084, China.
Chen
Bo
B
Meng
Fan-bo
FB
Ma
Hui
H
Chen
Die-yan
DY