title: Histone octamer instability under single molecule experiment conditions.
abstract: We have studied the sample concentration-dependent and external stress-dependent stability of native and reconstituted nucleosomal arrays. Whereas upon stretching a single chromatin fiber in a solution of very low chromatin concentration the statistical distribution of DNA length released upon nucleosome unfolding shows only one population centered around approximately 25 nm, in nucleosome stabilizing conditions a second population with average length of approximately 50 nm was observed. Using radioactively labeled histone H3 and H2B, we demonstrate that upon lowering the chromatin concentration to very low values, first the linker histones are released, followed by the H2A-H2B dimer, whereas the H3-H4 tetramer remains stably attached to DNA even at the lowest concentration studied. The nucleosomal arrays reconstituted on a 5 S rDNA tandem repeat exhibited similar behavior. This suggests that the 25-nm disruption length is a consequence of the histone H2A-H2B dimer dissociation from the histone octamer. In nucleosome stabilizing conditions, a full approximately 145 bp is constrained in the nucleosome. Our data demonstrate that the nucleosome stability and histone octamer integrity can be severely degraded in experiments where the sample concentration is low.
authors.affiliation: Laboratoire de Spectrometrie Physique, CNRS, UMR 5588, BP87, St. Martin d'Heres, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Claudet
authors.forename: Dimitar
authors.initials: D
authors.lastname: Angelov
authors.forename: Philippe
authors.initials: P
authors.lastname: Bouvet
authors.forename: Stefan
authors.initials: S
authors.lastname: Dimitrov
authors.forename: Jan
authors.initials: J
authors.lastname: Bednar
mesh_terms: D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004355:Drug Stability; D004548:Elasticity; D004912:Erythrocytes / Q000737:chemistry; D006657:Histones / Q000737:chemistry*; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D009707:Nucleosomes / Q000737:chemistry; D020836:Protein Structure, Quaternary; D012341:RNA, Ribosomal, 5S / Q000235:genetics
chemical_list: D002843:Chromatin; D004275:DNA, Ribosomal; D006657:Histones; D046911:Macromolecular Substances; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S