[Anti-proliferation effect of genistein on salivary adenoid cystic carcinoma cell line SACC-83 in vitro].
OBJECTIVE
To investigate the anti-proliferation effect of tyrosine protein kinase inhibitor, genistein (4'-5,7-trihydroxyisoflavone), on human salivary adenoid cystic carcinoma cell line SACC-83 in vitro, and its effect on cell cycle.


METHODS
SACC-83 cells cultured in vitro were treated with genistein,cell survival rate was calculated with MTT assay, phase contrast microscope was used to observe the status of cell growth and the morphological changes in each group, and cell cycle was detected with flow cytometry. Apoptosis was detected with Annexin V/PI staining. The results were statistically analyzed by SPSS11.5 software.


RESULTS
When treated with genistein of certain concentration for certain time, SACC-83 cell growths were significantly inhibited. With the increase of concentration of genistein and elongation of acting time, the inhibitory effects increased. Cells treated with genistein showed changes in morphology, cells' volume was decreased ,and suspended cells increased. Treated with 220 micromol/L genistein for 72 hours, SACC-83 cell growths were significantly inhibited, cell cycle was arrested in G2/M phase, and cell apoptosis was induced(P<0.01).


CONCLUSIONS
Genistein inhibits growth of human salivary adenoid cystic carcinoma cell line SACC-83, arrests cell cycle in G2/M phase, and induces cell apoptosis; these suggest that tyrosine protein kinase has important effect on the neoplasia and development of salivary adenoid cystic carcinoma.
Ma
Jie
J
Department of Oral Medicine,the Second Hospital of Chinese Medical University.Shenyang 110004, Liaoning Province, China. zgbl_2003@163.com
Zhong
Ming
M
Wang
Zhao-Yuan
ZY
D017209:Apoptosis / Q000187:drug effects; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D012468:Salivary Gland Neoplasms / Q000473:pathology*
D019833:Genistein