Harmonic phase-dispersion microscope with a Mach-Zehnder interferometer.
Harmonic phase-dispersion microscopy (PDM) is a new imaging technique in which contrast is provided by differences in refractive index at two harmonically related wavelengths. We report a new configuration of the harmonic phase-dispersion microscope in a Mach-Zehnder geometry as an instrument for imaging biological samples. Several improvements on the earlier design are demonstrated, including a single-pass configuration and acousto-optic modulators for generating the heterodyne signals without mechanical arm scanning. We demonstrate quantitative phase-dispersion images of test structures and biological samples.
Ahn
Andrew
A
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Yang
Changhuei
C
Wax
Adam
A
Popescu
Gabriel
G
Fang-Yen
Christopher
C
Badizadegan
Kamran
K
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D004867:Equipment Design; D019544:Equipment Failure Analysis; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D019697:Onions / Q000166:cytology*; D019047:Phantoms, Imaging