Increased expression of osteopontin gene in atypical teratoid/rhabdoid tumor of the central nervous system.
The atypical teratoid/rhabdoid tumor, primary to the central nervous system, is a highly malignant and aggressive neoplasm of infancy and childhood. Although having distinct biological features and clinical outcomes, it is frequently misdiagnosed as primitive neuroectodermal tumor/medulloblastoma. To further distinguish the underlying pathogenesis and to identify biological markers for clinical use, an atypical teratoid/rhabdoid tumor-derived cell line was established and its gene expression pattern analyzed in comparison to the human astrocyte SVG12 cell line and the human DAOY medulloblastoma cell line using a complementary DNA microarray method. The osteopontin gene was found specifically upregulated in atypical teratoid/rhabdoid tumor cells. This specificity was confirmed by immunohistochemistry in pathological sections of tissues from atypical teratoid/rhabdoid tumor patients. Even though the role of osteopontin in the cytopathogenesis of atypical teratoid/rhabdoid tumor still needs to be determined, our data support that overexpressed osteopontin is a potential diagnostic marker for atypical teratoid/rhabdoid tumor.
Kao
Chung-Lan
CL
Department of Physical Medicine & Rehabilitation, Taipei Veterans General Hospital, Taiwan, ROC.
Chiou
Shih-Hwa
SH
Chen
Yann-Jang
YJ
Singh
Sher
S
Lin
Han-Tso
HT
Liu
Ren-Shyan
RS
Lo
Chih-Wen
CW
Yang
Chi-Chang
CC
Chi
Chin-Wen
CW
Lee
Chen-hsen
CH
Wong
Tai-Tong
TT
D002460:Cell Line; D045744:Cell Line, Tumor; D016543:Central Nervous System Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008527:Medulloblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D053495:Osteopontin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018335:Rhabdoid Tumor / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012795:Sialoglycoproteins / Q000032:analysis / Q000235:genetics*; D013724:Teratoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin