Identification of intelectin overexpression in malignant pleural mesothelioma by serial analysis of gene expression (SAGE).
Malignant pleural mesothelioma (MPM) is a fatal neoplasm with no acceptable curative approaches. We used serial analysis of gene expression (SAGE) to compare the gene expression pattern of a surgically resected MPM to the autologous normal mesothelium. Intelectin gene overexpression (>139-fold) was found in the tumor. Online SAGE datasets revealed intelectin to be consistently present in mesothelioma(s), ovarian cancer, and colon cancer. Intelectin mRNA expression was found by RT-PCR in 4 of 5 resected MPM tumors, and Intelectin protein expression was confirmed by immunohistochemistry in 28 of 53 MPM tumors, and in 4 of 4 mesothelioma cell lines studied by Western blot. A marked induction in intelectin gene expression was observed among human primary mesothelial cells as a consequence of crocidolite asbestos exposure and simian virus 40 infection. Intelectin overexpression in mesothelioma could have potential screening, and therapeutic implications.
Wali
Anil
A
Thoracic Oncology Program, Karmanos Cancer Institute, and John D. Dingell VA Medical Center, Wayne State University, 3990 John R, Suite 2102, Detroit, MI 48201, USA.
Morin
Patrice J
PJ
Hough
Colleen D
CD
Lonardo
Fulvio
F
Seya
Tsukasa
T
Carbone
Michele
M
Pass
Harvey I
HI
D017638:Asbestos, Crocidolite / Q000009:adverse effects; D015153:Blotting, Western; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000235:genetics; D016207:Cytokines; D030541:Databases, Genetic; D005260:Female; D058851:GPI-Linked Proteins; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000096:biosynthesis* / Q000235:genetics; D008654:Mesothelioma / Q000235:genetics* / Q000601:surgery; D010051:Ovarian Neoplasms / Q000235:genetics; D010997:Pleural Neoplasms / Q000235:genetics* / Q000601:surgery; D027601:Polyomavirus Infections / Q000150:complications; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000150:complications
D016207:Cytokines; D058851:GPI-Linked Proteins; C432397:ITLN1 protein, human; D037102:Lectins; D012333:RNA, Messenger; D017638:Asbestos, Crocidolite