Short tandem repeat (STRs) and sex specific Amelogenin analysis of blood samples from neurosurgical female transfused patients.
Blood transfusion and its effects on DNA profiles of an individual is an important issue in the forensic context. In the present study, the effects of unfiltered whole male blood transfusion in seven female subjects were investigated by PCR-based assays on serial post-transfusion blood samples. PCR analysis of specific short tandem repeat (STR) primer pair to CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA and sex specific Amelogenin loci for banding pattern of alleles generated from the pre- and post-transfusion of seven female recipients and their corresponding donors were studied. Pre- and post-transfusion DNA profiles of all the seven female recipients were found to be consistent with no evidence of the male and female donor genetic material. It is concluded that currently used PCR-based STRs and sex specific Amelogenin loci DNA profiling techniques in forensic science are reliable and informative for paternity and identity purposes in situations of transfusion of 1 or 2 U of unfiltered whole male and female blood (> 72 h old) to female recipient up to 24 h post-transfusion.
Mukherjee
Kanchan Kumar
KK
Department of Neurosurgery, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Biswas
Rajesh
R
D000483:Alleles; D053523:Amelogenin; D001803:Blood Transfusion; D004247:DNA / Q000097:blood / Q000302:isolation & purification; D016172:DNA Fingerprinting; D017931:DNA Primers; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D019635:Neurosurgical Procedures; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis; D020080:Tandem Repeat Sequences; D014083:Tooth Germ
D053523:Amelogenin; D017931:DNA Primers; D003746:Dental Enamel Proteins; D004247:DNA