title: Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus.
abstract: Analysis of the genome of the human pathogen, Aspergillus fumigatus, revealed the presence of several putative glutathione transferase (GST) open reading frames. Three A. fumigatus GST genes, termed gstA, B, and C, were cloned and recombinant proteins expressed in Escherichia coli. Functional analysis of recombinant gstA-C confirms that the enzymes exhibit GST activity and glutathione peroxidase activity. RT-PCR confirmed low basal expression of gstA and gstC which was markedly up-regulated (at least 4x-10x) in the presence of either H2O2 or 1-chloro-2,4-dinitrobenzene (CDNB). GstB expression was only observed in the presence of CDNB. These results demonstrate for the first time the existence of three functional GSTs in A. fumigatus and strongly suggest a role for these enzymes in the response of the organism to both oxidative stress and xenobiotic presence.
authors.affiliation: National Institute for Cellular Biotechnology, Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland.
authors.forename: Claire
authors.initials: C
authors.lastname: Burns
authors.forename: Rachel
authors.initials: R
authors.lastname: Geraghty
authors.forename: Claire
authors.initials: C
authors.lastname: Neville
authors.forename: Alan
authors.initials: A
authors.lastname: Murphy
authors.forename: Kevin
authors.initials: K
authors.lastname: Kavanagh
authors.forename: Sean
authors.initials: S
authors.lastname: Doyle
mesh_terms: D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004137:Dinitrochlorobenzene / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D004137:Dinitrochlorobenzene; D005656:Fungal Proteins; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide; D005982:Glutathione Transferase