Multiquantum EPR spectroscopy of spin-labeled arrestin K267C at 35 GHz.
Three- and five-quantum absorption and dispersion multiquantum electron paramagnetic resonance spectra of a spin-labeled protein have been obtained for the first time at Q-band (35 GHz). Spectra of arrestin spin-labeled at site 267 were recorded at room temperature as a function of microwave power. The separation of irradiating microwave frequencies, Deltaf, was 10 kHz, and a newly-designed multiquantum Q-band electron paramagnetic resonance bridge was utilized, operating in a superheterodyne detection mode. The sample volume was 30 nL using a 3-loop-2-gap resonator. Most spectra were obtained at a 300 microM concentration in single, 2-min scans, but spectra were also successfully obtained at 30 microM, corresponding to one picomole of protein. Enhanced sensitivity to T(1) and T(2) was evident in the spectra, and linewidths varied considerably across the spectra. The pure absorption displays are beneficial relative to field modulation methods for spectral characterization. The presence of two states of the nitroxide spin-label with different relaxation times is evident, particularly in the dispersion spectra, which are expected to exhibit enhanced sensitivity to lineshape variation relative to absorption. Feasibility has been established for the use of this technique for site-directed spin-labeling studies of biologically relevant samples, particularly the study of protein structure and dynamics.
Klug
Candice S
CS
Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA. candice@mcw.edu
Camenisch
Theodore G
TG
Hubbell
Wayne L
WL
Hyde
James S
JS
D000042:Absorption; D000818:Animals; D019390:Arrestins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001703:Biophysics / Q000379:methods*; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D008872:Microwaves; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry; D011789:Quantum Theory; D017948:Retinal Rod Photoreceptor Cells; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D013113:Spin Labels; D019097:Spin Trapping; D013696:Temperature; D013997:Time Factors
D019390:Arrestins; D011506:Proteins; D013113:Spin Labels