title: Defining antigen-dependent stages of T cell migration from the blood to the central nervous system parenchyma.
abstract: In experimental autoimmune encephalomyelitis (EAE), intravenous transfer of activated CD4(+) myelin-specific T cells is sufficient to induce disease. Transferred T cells access the CNS parenchyma by trafficking across the blood brain barrier (BBB) vascular endothelium into the perivascular space, and then across the glial limitans that is made up of astrocytes and microglia. Flow cytometry analysis of cells isolated from CNS tissue does not distinguish between T cell populations at the various stages of migration. In this study, we have used GK1.5 (anti-CD4) treatment along with immunohistochemistry to distinguish between populations of T cells that are associated with the vasculature, T cells that have migrated into the perivascular space, and T cells in the parenchyma. We have also re-evaluated antigen specificity requirements of T cells as they are recruited to the CNS parenchyma. Activated myelin-specific T cells are restricted to the CNS vasculature for at least 24 h post transfer. MHC class II expression on the recipient is required for cells to traffic across the CNS vascular endothelium. Further, Con A-stimulated or non-CNS-specific (ovalbumin-specific) T cells fail to migrate into the perivascular space, and only enter the CNS parenchyma when co-transferred with myelin-specific T cells. Our results indicate that Th1 populations cannot accumulate in the perivascular (subarachnoid, Virchow-Robbins) space without a CNS antigen-specific signal.
authors.affiliation: Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Angela S
authors.initials: AS
authors.lastname: Archambault
authors.forename: Julia
authors.initials: J
authors.lastname: Sim
authors.forename: Mary Ann T
authors.initials: MA
authors.lastname: Gimenez
authors.forename: John H
authors.initials: JH
authors.lastname: Russell
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001769:Blood / Q000276:immunology*; D001812:Blood-Brain Barrier / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D002490:Central Nervous System / Q000276:immunology*; D004730:Endothelium, Vascular / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000941:Antigens; D000949:Histocompatibility Antigens Class II