title: Ocular inflammation alters swelling and membrane characteristics of rat Müller glial cells.
abstract: Ocular inflammation is a common cause of retinal edema that may involve swelling of Müller glial cells. In order to investigate whether endotoxin-induced ocular inflammation in rats alters the swelling and membrane characteristics of Müller cells, lipopolysaccharide (LPS; 0.5%) was intravitreally injected. At 3 and 7 days after treatment, hypotonic challenge induced swelling of Müller cell somata that was not observed in non-treated control eyes. Müller cells of LPS-treated eyes displayed a downregulation of inward K(+) currents and upregulation of A-type K(+) currents that was associated with a decreased expression of Kir4.1 protein in retinal slices. The data suggest that ocular inflammation induces alterations of both the swelling characteristics and the K(+) channel expression of Müller cells.
authors.affiliation: Paul Flechsig Institute of Brain Research, University of Leipzig Medical Faculty, D-04109 Leipzig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pannicke
authors.forename: Ortrud
authors.initials: O
authors.lastname: Uckermann
authors.forename: Ianors
authors.initials: I
authors.lastname: Iandiev
authors.forename: Peter
authors.initials: P
authors.lastname: Wiedemann
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.forename: Andreas
authors.initials: A
authors.lastname: Bringmann
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D025802:Electric Capacitance; D004558:Electric Stimulation; D018846:Excitatory Amino Acids / Q000494:pharmacology; D005128:Eye Diseases / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology; D007150:Immunohistochemistry / Q000379:methods; D008070:Lipopolysaccharides; D008564:Membrane Potentials / Q000502:physiology* / Q000528:radiation effects; D009457:Neuroglia / Q000473:pathology*; D018408:Patch-Clamp Techniques / Q000379:methods; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000473:pathology; D000098854:Kcnj10 Channel
chemical_list: D018846:Excitatory Amino Acids; D005904:Glial Fibrillary Acidic Protein; D000098854:Kcnj10 Channel; D008070:Lipopolysaccharides; D024661:Potassium Channels, Inwardly Rectifying; D018698:Glutamic Acid; D011189:Potassium Chloride