[Effect of high performance liquid chromatographic instrument system on the analysis of erythromycin A oxime].
A HPLC chromatography for the determination of erythromycin A oxime and relative compounds was studied, and the effect of chromatography systems including a HITACHI L-7100, a Shimadzu LC-6A, a Waters 474 and relative columns was analyzed. It was revealed that different HPLC apparatus and columns have obvious impact on the peak separation and retention time under the general chromatographic condition. The suitable chromatographic conditions for several different chromatography systems were summarized with good linear relationship, which is very significant to the quality control of erythromycin A oxime and relative compounds.
Sun
Jing-gu
JG
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.
Yao
Guo-wei
GW
Ou
Yu-xiang
YX
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004864:Equipment and Supplies; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis
D010091:Oximes; D004364:Pharmaceutical Preparations; C063102:erythromycin A oxime 11,12-carbonate; D004917:Erythromycin