[Experimental study on repairing segmental bone defects with three bio-bone derived materials].
OBJECTIVE
To evaluate the osteogenesis of three bio-bone derived materials in repairing segmental bone defects.


METHODS
Sixty Japanese rabbits were made 10 mm radius segmental defects and divided into 5 groups (groups A, B, C, D and E, n=12). Composite fully deproteinised bone (CFDB, group A), partially deproteinised bone (PDPB, group B), partially decalcified bone (PDCB, group C), autogenous iliac bone graft (group D) and no implant (group E) were implanted into the radius segmental bone defects of rabbits. The specimens were examined after 4, 8, 12 and 24 weeks; the osteogenesis was evaluated through X-ray radiograph and undecalcified solid tissue histological examination.


RESULTS
The border between the material and host's bone was distinct after 4 weeks and blurred after 8 weeks; the density of partial edge of the material was similar to that of radii after 12 weeks. The medullary cavity of bone reopened in group B; the density of most defect area was similar to that of the host bone and there was a few high density shadow in group C; the density of most defect area was higher than that of host bone in group A after 24 weeks. There was no significant difference in radiograph scoring between groups A, B and C after 4 weeks and 8 weeks (P>0.05); the scores of group B and C were higher than that of group A after 12 weeks (P<0.05); and the scores were arranged as follow: group D > group B > group C > group A after 24 weeks (P<0.05). Bone callus grew toward defect area and new bone adhered to the material after 4 weeks and 8 weeks; more new bone formed, and the materials were absorbed and degraded with time. The quantity of bone formation was more in group D than in group B and in group B than in group C and in group C than in group A after 24 weeks (P<0.05).


CONCLUSIONS
PDPB had good osteogenesis in repairing the segmental bone defect, PDCB was inferior to it, both PDPB and PDCB are fit to repair segmental bone defect. Both of them were inferior to autogenous bone.
Li
Yanlin
Y
Department of Orthopedic Surgery, the First Affiliated Hospital of Kunming Medical Colledge, Kunming Yunnan, 650032, PR China. liyanlinhan@yahoo.com.cn
Yang
Hao
H
Han
Rui
R
Li
Linzhi
L
Li
Xiuqun
X
Li
Shihe
S
Zhao
Xiaoxia
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000295:instrumentation* / Q000379:methods; D005260:Female; D008297:Male; D010012:Osteogenesis; D011817:Rabbits; D011884:Radius / Q000293:injuries / Q000503:physiopathology / Q000601:surgery*; D011897:Random Allocation; D023822:Tissue Engineering
D001672:Biocompatible Materials; D018786:Bone Substitutes