title: Detection and identification of spirolides in norwegian shellfish and plankton.
abstract: Mussels sampled in the spring of 2002 and 2003 from Skjer, a location in Sognefjord, Norway, tested positive in the mouse bioassay for lipophilic toxins. The symptoms, which included cramps, jumping, and short survival times (as low as 4 min), were not characteristic of toxins previously observed in Norway. A survey of the algae present at the aquaculture sites showed that the toxicity correlated with blooms of Alexandrium ostenfeldii. Up to 2200 cells/L were found at the peak of one bloom. In Canadian waters, this alga is known to be a producer of the cyclic imine toxins, spirolides. Analysis of mussel extracts from Skjer in the spring of 2002 and 2003, using liquid chromatography tandem mass spectrometry, revealed the presence of several new spirolides. The same compounds were also found in algal samples dominated by A. ostenfeldii, which had been sampled from Skjer in February 2003. A large-scale extraction of mussel digestive glands and chromatographic fractionation of the extracts allowed the isolation and structure elucidation of the main spirolide, 20-methyl spirolide G, with a molecular weight of 705.5. This is the first confirmed occurrence of spirolides in mussels and plankton from Norway.
authors.affiliation: Norwegian School of Veterinary Science, Department of Food Safety and Infection Biology, P.O. Box 8146 Dep., 0033 Oslo, Norway. john.aasen@veths.no
authors.forename: John
authors.initials: J
authors.lastname: Aasen
authors.forename: Shawna L
authors.initials: SL
authors.lastname: MacKinnon
authors.forename: Patricia
authors.initials: P
authors.lastname: LeBlanc
authors.forename: John A
authors.initials: JA
authors.lastname: Walter
authors.forename: Peter
authors.initials: P
authors.lastname: Hovgaard
authors.forename: Tore
authors.initials: T
authors.lastname: Aune
authors.forename: Michael A
authors.initials: MA
authors.lastname: Quilliam
mesh_terms: D000080:Acetals / Q000302:isolation & purification*; D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D004141:Dinoflagellida / Q000737:chemistry; D005260:Female; D007783:Lactones / Q000302:isolation & purification*; D008387:Marine Toxins / Q000302:isolation & purification*; D051379:Mice; D009664:Norway; D010933:Plankton / Q000737:chemistry*; D012758:Shellfish / Q000032:analysis*; D013141:Spiro Compounds / Q000302:isolation & purification*
chemical_list: C503463:20-methyl spirolide G; D000080:Acetals; D007783:Lactones; D008387:Marine Toxins; D013141:Spiro Compounds