Immunodetection of anti-MAG IgM antibody by cross-reactivity to LA-N-1 neuroblastoma cells.
Demyelinating polyneuropathiy associated with IgM paraproteinemia and high titers of anti-MAG IgM antibodies (MAG-PN) is considered different from chronic inflammatory demyelinating polyneuropathy, particularly because of the poorer response to treatment of MAG-PN patients. Therefore, anti-MAG anitbodies may have relevant prognostic value. Available anti-MAG antibody assays require central nervous system myelin proteins from autopsied human brains. This study investigated the feasibility of detecting anti-MAG antibody by immunofluorescence and flow cytometry using a panel of human neuroblastoma cell lines as targets. We report here on the evaluation of the LA-N-1 cell line as an appropriate substrate for the detection of anti-MAG antibody by indirect immunoflourescence.
Isoardo
Gianluca
G
SOC Neurologia, Ospedale Civile di Asti, 14100 Asti, Italy. gianlucaisoardo@yahoo.it
Deaglio
Silvia
S
Cocito
Dario
D
Migliaretti
Giuseppe
G
Ferrero
Enza
E
Cavallo
Franco
F
Durelli
Luca
L
Malavasi
Fabio
F
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000378:metabolism*; D001667:Binding, Competitive / Q000276:immunology; D018959:CD57 Antigens / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D003710:Demography; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008297:Male; D008875:Middle Aged; D018992:Myelin-Associated Glycoprotein / Q000276:immunology* / Q000378:metabolism; D009447:Neuroblastoma / Q000276:immunology / Q000378:metabolism*; D020277:Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / Q000097:blood / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D018959:CD57 Antigens; D018992:Myelin-Associated Glycoprotein; C044203:anti-IgM