[TLC-FT-SERS study on a pair of optic isomers in ephedra].
A new method for analyzing the ingredients of a pair of optic isomers in ephedra, nor-ephedrine and nor-pseudo-ephedrine, using hyphenated high-efficiency thin layer chromatography (TLC) and surface-enhanced Raman spectroscopy (SERS) techniques, is reported. The results show that the characteristic spectral bands of nor-ephedrine and nor-pseudo-ephedrine can be obtained from the TLC spot with 8 microg sample of about 2.0 mm in diameter. The difference between the SERS and solid spectra was found. Spectral bands at 1004 cm(-1) and 1605 cm(-1) were found greatly enhanced. Molecule was absorbed in surface silver sol by pi electrons in ring. Under similar experimental conditions the spectral information of Levo-nor-ephedrine ramifications TLC-SERS is rich with strong credibility, whereas dextral-nor-ephedrine ramifications show a relatively strong fluorescence backdrop with less spectral information and weak credibility. The effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.
Wang
Yuan
Y
Department of Chemistry, Capital Normal University, Beijing 100037, China.
Zhang
Jin-zhi
JZ
Ma
Xin-yong
XY
D018663:Adrenergic Agents / Q000737:chemistry / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D029789:Ephedra / Q000737:chemistry*; D004809:Ephedrine / Q000737:chemistry* / Q000302:isolation & purification; D007536:Isomerism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D018663:Adrenergic Agents; D004365:Drugs, Chinese Herbal; D004809:Ephedrine