Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil.
The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.
Amorim
Regina M S
RM
Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, ICC Sul, 70919-900 Brasília, DF, Brazil.
Oliveira
Claudiner P
CP
Wyant
Patrícia S
PS
Cerqueira
Daniela M
DM
Câmara
Geni N L
GN
Flores
Luciano S
LS
Martins
Regina M B
RM
Martins
Cláudia R F
CR
D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000276:immunology; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018937:Hepatitis C Antibodies