title: Vibrio cholerae persistence in aquatic environments and colonization of intestinal cells: involvement of a common adhesion mechanism.
abstract: Forty-one Tnpho A mutants of Vibrio cholerae O1 classical strain CD81 were analyzed for their ability to interact with chitin particles, Tigriopus fulvus copepods and the Intestine 407 cell line compared to the parent strain. Thirteen mutants were less adhesive than CD81; in particular, T21, T33 and T87 were less adhesive towards all substrates and insensitive to inhibition by N-acetyl glucosamine (GlcNAc). By SDS-PAGE analysis of sarkosyl-insoluble membrane proteins (siMPs) isolated from mutants and parent, it was found that a 53 kDa siMP is missing in T21, T33 and T87 mutants. It is hypothesized that this protein might have the function to mediate adherence to GlcNAc-containing substrates both in the aquatic environment and in human intestine.
authors.affiliation: Istituto di Microbiologia e Scienze Biomediche, Università Politecnica delle Marche, Ancona 60131, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Zampini
authors.forename: Carla
authors.initials: C
authors.lastname: Pruzzo
authors.forename: Vijay P
authors.initials: VP
authors.lastname: Bondre
authors.forename: Renato
authors.initials: R
authors.lastname: Tarsi
authors.forename: Mariangela
authors.initials: M
authors.lastname: Cosmo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bacciaglia
authors.forename: Arvind
authors.initials: A
authors.lastname: Chhabra
authors.forename: Renjana
authors.initials: R
authors.lastname: Srivastava
authors.forename: Brahm S
authors.initials: BS
authors.lastname: Srivastava
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002686:Chitin / Q000502:physiology*; D033342:Copepoda / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D014734:Vibrio cholerae / Q000502:physiology*
chemical_list: D002686:Chitin