title: Comparative proteome analysis of breast cancer and normal breast.
abstract: Breast cancer is a leading cause of death for women. The underlying molecular mechanism is still not well understood. In this study, two-dimensional gel electrophoresis combined with mass spectrometry was used to analyze changes in the proteome of infiltrating ductal carcinoma compared to normal breast tissue. Ten sets of two-dimensional gels per experimental condition were analyzed and more than 500 spots each were detected. This revealed 39 spots for which expression in breast cancer cells were reproducibly altered more than twofold compared to normal controls (p < 0.01). These spots represented 25 different proteins after identification using the database search after mass spectrometry, comprising cell defense proteins, enzymes involved in glycolytic energy metabolism and homeostasis, protein folding and structural proteins, proteins involved in cytoskeleton and cell motility, and proteins involved in other functions. In addition, 28 nondifferentially expressed proteins with different functions were also mapped and identified, which might help to establish a two-dimensional gel electrophoresis reference map of human breast cancer. Our study shows that proteomics offers a powerful methodology to detect the proteins that show different expression patterns in breast cancer tissue and may provide an accurate molecular classification. The differentially expressed proteins may be used as potential candidate markers for diagnostic purposes or for determination of tumor sensitivity to therapy. The functional implications of the identified proteins are discussed.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Med Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Luo
authors.forename: Jindan
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Liu
authors.forename: Allan Christian
authors.initials: AC
authors.lastname: Shaw
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Wang
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Wu
authors.forename: Xuan
authors.initials: X
authors.lastname: Zeng
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Youhe
authors.initials: Y
authors.lastname: Gao
authors.forename: Dexian
authors.initials: D
authors.lastname: Zheng
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020543:Proteome / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020543:Proteome