Effect of antibodies against intercellular adhesion molecule-1, B7, and CD40 on interleukin-18-treated human mixed lymphocyte reaction.
The interleukin (IL)-18 level in plasma is elevated during the acute rejection after organ transplantation. IL-18 elicits adhesion molecule expression as well as interferon-gamma/IL-12 production and T-cell proliferation in the human mixed lymphocyte reaction, an in vitro model of acute rejection. We examined whether antibodies (Abs) against intercellular adhesion molecule (ICAM)-1, B7, CD40, and CD40, ligand (CD40L) affect the cytokine production and T-cell proliferation. Anti-ICAM-1 and B7 Abs suppressed the cytokine production, while all Abs inhibited T-cell proliferation. ICAM-1 and B7 as well as CD40 may play different roles in the acute rejection.
Takahashi
Hideo Kohka
HK
Department of Pharmacology, Okayama University Graduate School of Medicine and Dentistry, Okayama City.
Iwagaki
Hiromi
H
Tamura
Ryuji
R
Yagi
Takahito
T
Yoshino
Tadashi
T
Mori
Shuji
S
Tanaka
Noriaki
N
Nishibori
Masahiro
M
D000906:Antibodies / Q000378:metabolism*; D018122:B7-1 Antigen / Q000187:drug effects / Q000378:metabolism*; D019013:CD40 Antigens / Q000187:drug effects / Q000378:metabolism*; D023201:CD40 Ligand / Q000187:drug effects / Q000378:metabolism; D015815:Cell Adhesion Molecules; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000187:drug effects; D018664:Interleukin-12 / Q000096:biosynthesis; D020382:Interleukin-18 / Q000494:pharmacology*; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000187:drug effects
D000906:Antibodies; D018122:B7-1 Antigen; D019013:CD40 Antigens; D015815:Cell Adhesion Molecules; D020382:Interleukin-18; D018799:Intercellular Adhesion Molecule-1; D023201:CD40 Ligand; D018664:Interleukin-12; D007371:Interferon-gamma