[Expression of interleukin 2 and IL-2 receptor after implanted tissue engineered bones constructed with allogeneic marrow stromal stem cells and bio-derived materials in rhesus monkeys].
OBJECTIVE
To explore the feasibility of allogeneic marrow stromal stem cells (MSCs) as seed cells to construct tissue engineered bone by detecting the expressions of interleukin 2 (IL-2) and IL-2 receptor in rhesus monkeys after implanting these tissue engineered bones.


METHODS
Engineered bones were constructed with osteoblasts which derived from allogeneic MSCs and bio-derived materials in vitro, and then were implanted to bridge 2.5 cm segmental bone defects of left radius in 15 rhesus monkeys as experimental group, bio-derived materials only were implanted to bridge same size defects of right radius as control group. Every 3 monkeys were sacrificed in the 1st, the 2nd, the 3rd, the 6th and the 12th weeks postoperatively and the expressions of IL-2 and IL-2 receptor in blood and graft samples were detected quantitatively by enzyme-linked immunosorbent assay (ELISA).


RESULTS
There was no significant difference in the contents of IL-2 and its receptor between 2 groups (P>0.05). The contents of IL-2 and its receptor increased from the 2nd week and maintained high level from the 2nd to the 6th week, but decreased after 6 weeks.


CONCLUSIONS
Tissue engineered bones constructed with allogeneic MSCs and bio-derived materials show low immunogenicity. Allogeneic MSCs may be used as seed cells to construct tissue engineered bone.
Yang
Fuchun
F
Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China.
Yang
Zhiming
Z
Li
Xiuqun
X
Zhou
Yueting
Y
Lin
Fan
F
Qin
Tingwu
T
Luo
Jingcong
J
Xie
Huiqi
H
D000818:Animals; D001672:Biocompatible Materials; D016026:Bone Marrow Transplantation; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007376:Interleukin-2 / Q000097:blood / Q000378:metabolism*; D008253:Macaca mulatta; D011884:Radius / Q000293:injuries / Q000601:surgery; D015375:Receptors, Interleukin-2 / Q000097:blood / Q000378:metabolism*; D017154:Stromal Cells / Q000637:transplantation; D023822:Tissue Engineering / Q000379:methods; D014184:Transplantation, Homologous
D001672:Biocompatible Materials; D018786:Bone Substitutes; D007376:Interleukin-2; D015375:Receptors, Interleukin-2