title: Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR.
abstract: One and two-dimensional solid-state NMR experiments are discussed that permit probing local structure and overall molecular conformation of membrane-embedded polypeptides under Magic Angle Spinning. The functional dependence of a series of anisotropic recoupling schemes is analyzed using theoretical and numerical methods. These studies lead to the construction of a set of polarization dephasing or transfer units that probe local backbone conformation and overall molecular orientation within the same NMR experiment. Experimental results are shown for a randomly oriented peptide and for two model membrane-peptides reconstituted into lipid bilayers and oriented on polymer films according to a method proposed by Bechinger et al.
authors.affiliation: Department of NMR-Based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: O C
authors.initials: OC
authors.lastname: Andronesi
authors.forename: J R
authors.initials: JR
authors.lastname: Pfeifer
authors.forename: L
authors.initials: L
authors.lastname: Al-Momani
authors.forename: S
authors.initials: S
authors.lastname: Ozdirekcan
authors.forename: D T S
authors.initials: DT
authors.lastname: Rijkers
authors.forename: B
authors.initials: B
authors.lastname: Angerstein
authors.forename: S
authors.initials: S
authors.lastname: Luca
authors.forename: U
authors.initials: U
authors.lastname: Koert
authors.forename: J A
authors.initials: JA
authors.lastname: Killian
authors.forename: M
authors.initials: M
authors.lastname: Baldus
mesh_terms: D016880:Anisotropy; D006096:Gramicidin / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D008051:Lipid Bilayers; D010455:Peptides; D013113:Spin Labels; D006096:Gramicidin