title: Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers.
abstract: Hepatocellular carcinoma (HCC) is a malignancy of both underdeveloped and developing countries. Proteomes of ten pairs of clinical hepatitis B virus associated HCC tissue samples were obtained by high resolution two-dimensional gel electrophoresis. Comprehensive analyses of proteins associated with B-type HCC were focused on total differentially expressed proteins (> or = two-fold increase or decrease, Student's t-test, p < 0.05) from one pair of samples. Protein identification was done by peptide mass fingerprinting with matrix assisted laser desorption/ionization-time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry. Comparative analyses of proteins associated with B-type HCC included repeat statistics in ten cases. A total of 100 protein spots, corresponding to 80 different gene products, were identified. Proteins whose expression levels were different by more than 2-fold in at least 50% of the cases (five of ten cases) were further analyzed and 45 proteins were selected out as candidates for HCC-associated proteins. Western blotting further validated up-regulated expressions of two candidate proteins in tumor tissues: proliferating cell antigen and stathmin 1. This comprehensive and comparative analyses of proteins associated with B-type HCC could provide useful molecular markers for diagnostics and prognostics and for therapeutic targets. The physiological significance of the differential expressions for several candidate proteins are discussed.
authors.affiliation: Research Center for Proteome Analysis, Key Lab of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Ye-Xiong
authors.initials: YX
authors.lastname: Tan
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
authors.forename: Shi-Jian
authors.initials: SJ
authors.lastname: Ding
authors.forename: Su-Jun
authors.initials: SJ
authors.lastname: Li
authors.forename: Dan-Jun
authors.initials: DJ
authors.lastname: Ma
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Man
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.forename: Qi-Chang
authors.initials: QC
authors.lastname: Xia
authors.forename: Jia-Rui
authors.initials: JR
authors.lastname: Wu
authors.forename: Hong-Yang
authors.initials: HY
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000150:complications* / Q000821:virology*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D019295:Computational Biology / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D006509:Hepatitis B / Q000150:complications*; D006515:Hepatitis B virus / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008868:Microtubule Proteins / Q000096:biosynthesis; D008875:Middle Aged; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D020033:Protein Isoforms; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050777:Stathmin; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D008868:Microtubule Proteins; D010750:Phosphoproteins; D018809:Proliferating Cell Nuclear Antigen; D020033:Protein Isoforms; C493995:STMN1 protein, human; D050777:Stathmin