title: Sol i 1, the phospholipase allergen of imported fire ant venom.
abstract: BACKGROUND
  Sol i 1, the venom phospholipase of imported fire ant venom is an important allergen and exhibits some cross-reactivity with IgE antibodies from patients sensitized to other Hymenoptera venoms.
  
  
  OBJECTIVE
  To determine the primary structure of Sol i 1 and evaluate the roles of protein and carbohydrate epitopes in its cross-reactivity.
  
  
  METHODS
  Sol i 1 was purified from venom, proteolytic peptides prepared and amino acid sequences obtained. The cDNA for Sol i 1 was cloned, sequenced, and compared with sequences of other wasp venom phospholipases. The role of carbohydrate epitopes in the cross-reactivity with other Hymenoptera venoms was studied by RAST inhibition.
  
  
  RESULTS
  The sequence identified Sol i 1 as a lipase of the GX class, lipoprotein lipase superfamily, pancreatic lipase homologous family and RP2 subgroup phospholipases as are the vespid venom phospholipases. The 148 residues identified by amino acid sequencing represent about 48% of the translated cDNA sequence. Sol i 1 was 31-32% identical to yellow jacket phospholipases. The identical regions of sequence were clustered in the domain which forms the serine hydrolase active site. Mannosylated N-glycans could completely inhibit binding of IgE from honeybee venom sensitized patients to Sol i 1. Inhibition by glycan of IgE binding from yellow jacket venom sensitized patients was low or absent for three of eight sera and substantial, but not complete for five sera.
  
  
  CONCLUSIONS
  Sol i 1 is related to wasp venom phospholipases. Cross-reactivity with honeybee venom is caused by carbohydrate, whereas cross-reactivity with yellow jacket venom involves reactivity with both carbohydrate determinants of hyaluronidase and high molecular weight proteins and phospholipase protein determinants.
authors.affiliation: Department of Pathology and Laboratory Medicine, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA. hoffmand@mail.ecu.edu
authors.forename: Donald R
authors.initials: DR
authors.lastname: Hoffman
authors.forename: Rhonda H
authors.initials: RH
authors.lastname: Sakell
authors.forename: Margit
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000862:Ant Venoms / Q000737:chemistry*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D010740:Phospholipases / Q000737:chemistry* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011852:Radioallergosorbent Test; D017386:Sequence Homology, Amino Acid; D014862:Wasp Venoms / Q000737:chemistry
chemical_list: D000485:Allergens; D000862:Ant Venoms; D019476:Insect Proteins; D014862:Wasp Venoms; D010740:Phospholipases